LOCUS       DAZUMM010000001        83895 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000001 DAZUMM010000000
VERSION     DAZUMM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 83895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.001"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(188..2374)
                     /locus_tag="ACG8E6_000001"
     CDS             complement(188..2374)
                     /locus_tag="ACG8E6_000001"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFS5060462.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2592..3275
                     /locus_tag="ACG8E6_000002"
     CDS             2592..3275
                     /locus_tag="ACG8E6_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFS5060463.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3356..4075)
                     /locus_tag="ACG8E6_000003"
     CDS             complement(3356..4075)
                     /locus_tag="ACG8E6_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS5060464.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4387..4593)
                     /locus_tag="ACG8E6_000004"
     CDS             complement(4387..4593)
                     /locus_tag="ACG8E6_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060465.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4838..5440
                     /locus_tag="ACG8E6_000005"
     CDS             4838..5440
                     /locus_tag="ACG8E6_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS5060466.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5581..6861
                     /locus_tag="ACG8E6_000006"
     CDS             5581..6861
                     /locus_tag="ACG8E6_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS5060467.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            6874..7602
                     /locus_tag="ACG8E6_000007"
     CDS             6874..7602
                     /locus_tag="ACG8E6_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS5060468.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(7671..9194)
                     /locus_tag="ACG8E6_000008"
     CDS             complement(7671..9194)
                     /locus_tag="ACG8E6_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS5060469.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            9446..10627
                     /locus_tag="ACG8E6_000009"
     CDS             9446..10627
                     /locus_tag="ACG8E6_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5060470.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(10687..12315)
                     /gene="treC"
                     /locus_tag="ACG8E6_000010"
     CDS             complement(10687..12315)
                     /gene="treC"
                     /locus_tag="ACG8E6_000010"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFS5060471.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12766..15477)
                     /locus_tag="ACG8E6_000011"
     CDS             complement(12766..15477)
                     /locus_tag="ACG8E6_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFS5060472.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(15487..16188)
                     /locus_tag="ACG8E6_000012"
     CDS             complement(15487..16188)
                     /locus_tag="ACG8E6_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5060473.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16374..16973
                     /locus_tag="ACG8E6_000013"
     CDS             16374..16973
                     /locus_tag="ACG8E6_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS5060474.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            17054..18067
                     /locus_tag="ACG8E6_000014"
     CDS             17054..18067
                     /locus_tag="ACG8E6_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFS5060475.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            18175..19092
                     /gene="rbsK"
                     /locus_tag="ACG8E6_000015"
     CDS             18175..19092
                     /gene="rbsK"
                     /locus_tag="ACG8E6_000015"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFS5060476.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            19120..19515
                     /gene="rbsD"
                     /locus_tag="ACG8E6_000016"
     CDS             19120..19515
                     /gene="rbsD"
                     /locus_tag="ACG8E6_000016"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFS5060477.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            19515..20888
                     /locus_tag="ACG8E6_000017"
     CDS             19515..20888
                     /locus_tag="ACG8E6_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5060478.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            21245..23389
                     /locus_tag="ACG8E6_000018"
     CDS             21245..23389
                     /locus_tag="ACG8E6_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5060479.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(23441..24481)
                     /locus_tag="ACG8E6_000019"
     CDS             complement(23441..24481)
                     /locus_tag="ACG8E6_000019"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS5060480.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(24758..26560)
                     /locus_tag="ACG8E6_000020"
     CDS             complement(24758..26560)
                     /locus_tag="ACG8E6_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5060481.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(26574..27326)
                     /locus_tag="ACG8E6_000021"
     CDS             complement(26574..27326)
                     /locus_tag="ACG8E6_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5060482.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(27335..27697)
                     /locus_tag="ACG8E6_000022"
     CDS             complement(27335..27697)
                     /locus_tag="ACG8E6_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5060483.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(27873..28745)
                     /locus_tag="ACG8E6_000023"
     CDS             complement(27873..28745)
                     /locus_tag="ACG8E6_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS5060484.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(28796..29284)
                     /locus_tag="ACG8E6_000024"
     CDS             complement(28796..29284)
                     /locus_tag="ACG8E6_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS5060485.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(29422..30213)
                     /locus_tag="ACG8E6_000025"
     CDS             complement(29422..30213)
                     /locus_tag="ACG8E6_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFS5060486.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(30268..30876)
                     /locus_tag="ACG8E6_000026"
     CDS             complement(30268..30876)
                     /locus_tag="ACG8E6_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFS5060487.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(30869..31186)
                     /locus_tag="ACG8E6_000027"
     CDS             complement(30869..31186)
                     /locus_tag="ACG8E6_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5060488.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(31342..32070)
                     /locus_tag="ACG8E6_000028"
     CDS             complement(31342..32070)
                     /locus_tag="ACG8E6_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFS5060489.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32229..33110
                     /locus_tag="ACG8E6_000029"
     CDS             32229..33110
                     /locus_tag="ACG8E6_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5060490.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(33347..34390)
                     /locus_tag="ACG8E6_000030"
     CDS             complement(33347..34390)
                     /locus_tag="ACG8E6_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5060491.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(34393..35061)
                     /locus_tag="ACG8E6_000031"
     CDS             complement(34393..35061)
                     /locus_tag="ACG8E6_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5060492.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            35203..37539
                     /locus_tag="ACG8E6_000032"
     CDS             35203..37539
                     /locus_tag="ACG8E6_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFS5060493.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(37654..37965)
                     /locus_tag="ACG8E6_000033"
     CDS             complement(37654..37965)
                     /locus_tag="ACG8E6_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060494.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            38146..39621
                     /locus_tag="ACG8E6_000034"
     CDS             38146..39621
                     /locus_tag="ACG8E6_000034"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFS5060495.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            39668..40252
                     /locus_tag="ACG8E6_000035"
     CDS             39668..40252
                     /locus_tag="ACG8E6_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFS5060496.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            40254..40673
                     /locus_tag="ACG8E6_000036"
     CDS             40254..40673
                     /locus_tag="ACG8E6_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFS5060497.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(40954..41238)
                     /locus_tag="ACG8E6_000037"
     CDS             complement(40954..41238)
                     /locus_tag="ACG8E6_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFS5060498.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(41590..42300)
                     /locus_tag="ACG8E6_000038"
     CDS             complement(41590..42300)
                     /locus_tag="ACG8E6_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFS5060499.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(42297..43769)
                     /locus_tag="ACG8E6_000039"
     CDS             complement(42297..43769)
                     /locus_tag="ACG8E6_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS5060500.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            43907..44380
                     /locus_tag="ACG8E6_000040"
     CDS             43907..44380
                     /locus_tag="ACG8E6_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5060501.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(44533..45084)
                     /locus_tag="ACG8E6_000041"
     CDS             complement(44533..45084)
                     /locus_tag="ACG8E6_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5060502.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            45189..45719
                     /locus_tag="ACG8E6_000042"
     CDS             45189..45719
                     /locus_tag="ACG8E6_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFS5060503.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(46202..46645)
                     /locus_tag="ACG8E6_000043"
     CDS             complement(46202..46645)
                     /locus_tag="ACG8E6_000043"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5060504.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(46661..47821)
                     /locus_tag="ACG8E6_000044"
     CDS             complement(46661..47821)
                     /locus_tag="ACG8E6_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5060505.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            48328..50028
                     /locus_tag="ACG8E6_000045"
     CDS             48328..50028
                     /locus_tag="ACG8E6_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060506.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(50130..50963)
                     /locus_tag="ACG8E6_000046"
     CDS             complement(50130..50963)
                     /locus_tag="ACG8E6_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS5060507.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(51267..53432)
                     /locus_tag="ACG8E6_000047"
     CDS             complement(51267..53432)
                     /locus_tag="ACG8E6_000047"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFS5060508.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            53692..55986
                     /locus_tag="ACG8E6_000048"
     CDS             53692..55986
                     /locus_tag="ACG8E6_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFS5060509.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            55979..56644
                     /gene="pgmB"
                     /locus_tag="ACG8E6_000049"
     CDS             55979..56644
                     /gene="pgmB"
                     /locus_tag="ACG8E6_000049"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS5060510.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            56637..57644
                     /locus_tag="ACG8E6_000050"
     CDS             56637..57644
                     /locus_tag="ACG8E6_000050"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS5060511.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            57666..58685
                     /locus_tag="ACG8E6_000051"
     CDS             57666..58685
                     /locus_tag="ACG8E6_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5060512.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(58966..60333)
                     /gene="nhaC"
                     /locus_tag="ACG8E6_000052"
     CDS             complement(58966..60333)
                     /gene="nhaC"
                     /locus_tag="ACG8E6_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS5060513.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(60442..61863)
                     /gene="tyrP"
                     /locus_tag="ACG8E6_000053"
     CDS             complement(60442..61863)
                     /gene="tyrP"
                     /locus_tag="ACG8E6_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFS5060514.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(62106..63983)
                     /gene="tdc"
                     /locus_tag="ACG8E6_000054"
     CDS             complement(62106..63983)
                     /gene="tdc"
                     /locus_tag="ACG8E6_000054"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS5060515.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(64274..65530)
                     /gene="tyrS"
                     /locus_tag="ACG8E6_000055"
     CDS             complement(64274..65530)
                     /gene="tyrS"
                     /locus_tag="ACG8E6_000055"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFS5060516.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(65642..65847)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            66110..67411
                     /locus_tag="ACG8E6_000056"
     CDS             66110..67411
                     /locus_tag="ACG8E6_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS5060517.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(67480..68079)
                     /locus_tag="ACG8E6_000057"
     CDS             complement(67480..68079)
                     /locus_tag="ACG8E6_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5060518.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            68333..69214
                     /locus_tag="ACG8E6_000058"
     CDS             68333..69214
                     /locus_tag="ACG8E6_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5060519.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            69227..70837
                     /locus_tag="ACG8E6_000059"
     CDS             69227..70837
                     /locus_tag="ACG8E6_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5060520.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(70914..71573)
                     /locus_tag="ACG8E6_000060"
     CDS             complement(70914..71573)
                     /locus_tag="ACG8E6_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5060521.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(71856..72521)
                     /locus_tag="ACG8E6_000061"
     CDS             complement(71856..72521)
                     /locus_tag="ACG8E6_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFS5060522.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(72668..73273)
                     /gene="dhaL"
                     /locus_tag="ACG8E6_000062"
     CDS             complement(72668..73273)
                     /gene="dhaL"
                     /locus_tag="ACG8E6_000062"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFS5060523.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(73301..74311)
                     /gene="dhaK"
                     /locus_tag="ACG8E6_000063"
     CDS             complement(73301..74311)
                     /gene="dhaK"
                     /locus_tag="ACG8E6_000063"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFS5060524.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(74286..74681)
                     /gene="dhaM"
                     /locus_tag="ACG8E6_000064"
     CDS             complement(74286..74681)
                     /gene="dhaM"
                     /locus_tag="ACG8E6_000064"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFS5060525.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            74835..75155
                     /locus_tag="ACG8E6_000065"
     CDS             74835..75155
                     /locus_tag="ACG8E6_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFS5060526.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(75267..76124)
                     /locus_tag="ACG8E6_000066"
     CDS             complement(75267..76124)
                     /locus_tag="ACG8E6_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS5060527.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(76232..77536)
                     /locus_tag="ACG8E6_000067"
     CDS             complement(76232..77536)
                     /locus_tag="ACG8E6_000067"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5060528.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(77533..78042)
                     /locus_tag="ACG8E6_000068"
     CDS             complement(77533..78042)
                     /locus_tag="ACG8E6_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060529.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(78217..80352)
                     /locus_tag="ACG8E6_000069"
     CDS             complement(78217..80352)
                     /locus_tag="ACG8E6_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS5060530.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(80435..82090)
                     /locus_tag="ACG8E6_000070"
     CDS             complement(80435..82090)
                     /locus_tag="ACG8E6_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5060531.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(82092..82406)
                     /locus_tag="ACG8E6_000071"
     CDS             complement(82092..82406)
                     /locus_tag="ACG8E6_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS5060532.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            82576..82836
                     /locus_tag="ACG8E6_000072"
     CDS             82576..82836
                     /locus_tag="ACG8E6_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFS5060533.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            82850..83164
                     /locus_tag="ACG8E6_000073"
     CDS             82850..83164
                     /locus_tag="ACG8E6_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFS5060534.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT        24797 a        17039 c        15011 g        27048 t
ORIGIN      
        1 tcattttcaa aataaataag ggaggtgtga caaaattctt gtcacacctc ccttttccga
       61 ataaactgtg ttccagaaat agcttcttcg aaaataagct gaaatttcac aaaaatttga
      121 gaagcaattt tcgtacattc cttcttcttt cttgaaactg accacttctg tcacaccctc
      181 tcttctatca ctttcctttt ttcacacgac tcccggtttc tttgataaat ctcgatactt
      241 tcaattttct tgaatttttc atttgaggta cacatagata cagacgattt cttgctctag
      301 tcatcgcaac ataaaatagt cttcgttctt cttctatcgt cgttactggt tctgctttta
      361 tttcattaaa gtcgattact ttcttttctg tcgctccgtt ataaggcaaa cttccttcgt
      421 tacaaccaat caaaaagaca tttcggtaac taagaccttt acttttgtga atgctctgga
      481 tacatagtgc atcaagtttt ccttgtctgt aagcaatcaa gcgggttttt aattctttag
      541 ctgtcgcttg ggtttcttgc gataaggctt ggagtcttct gaaagtagca tgctgcttta
      601 cttcttctcg tatccattct tgatcactct ggtcgatttc cttagctata cgtggcagat
      661 gaagatagct ttgaacaagc gaatagattt cttgatgctg gtctgtttta cgcaacgctt
      721 cctcaaactc tcggatttct gcagatttcg attttaaatt ggggcttatc gacaaaatat
      781 aatctgcaac ggatatattt tgatatttag gagaaaagta ttgatcgcat tcactttttt
      841 tcaggtagca gtctgccatc agctgccgga gcaattgctg gtaggcctga tttttctttt
      901 gcagattcag tgctgtttgc caatgaacaa ttttttgata gaaatgttgg aaagacttga
      961 taccaaagat accttcatat ggactatctg cttccagtac aaaaggaata ttcgttttca
     1021 gcagagattg gacaagctgc tgcgtttgtg aagctgatcg gtataatatg attgattcct
     1081 tatatttccc atttttttga ataaatgtct gaatttgttt gattagccac tgactttctg
     1141 ctttagtatc agaaaacaaa taggcttcca taacacctgc ttctttttgt gctggaatca
     1201 tcgcctttgg tatccgatca tttttattat agctgatcaa tctatttgct ccttggacaa
     1261 tagctgcccg agaacgataa ttagttaata agaaaagagt tttggtagga tattcttttt
     1321 caaagttctt gatgtatgct ggctctgatc ctctgaatcc atagatactc tgatcatcat
     1381 ctccaacaac aatcaacttg atcctgtttt cctttgtcat ccgtttcacg atttcccatt
     1441 gaagtggatt agtatcttga aattcatcaa tgaatagata atcatatttt tggccgatat
     1501 aatgctggat ctcctcattc tccaaagcgc ttttcagatt ttggagtaca tcattgaact
     1561 ctagcaattg ctggcttctt ttttgttctt ggagttccgt gagcaatcca tacagtcgtt
     1621 ctgatatcgg ctgtcgtgca ttcttatcaa tggaatcata tttttcttta actaactgaa
     1681 ccatttcttc tactgaaagg ttcatattct gccaatagtc taaccgagaa gcaatcgttt
     1741 tttctccata ttgatcaaat atataaagat tatatttacg tatcagctgt tgcaaaacag
     1801 cattcacttt ttgtgcagta tcctttgaaa agtctccttg tattccttgc attgctactt
     1861 caggaaattt tcgacaaatt tcattgtata tcctaaaaaa gatactatgg aatgttccga
     1921 tatggacagc ggtttttctc tggctgaaat tatctactcg tttttttagt tcatttgcag
     1981 cgttccttgt aaaagtgatc attagtatgc gttcttcgtc tacttgtttt tgcgtcatca
     2041 gataaacagc ttttgcacag attgtttgcg ttttgccgct cccagctcct gctatgattc
     2101 gaagatgttt cttcatatca tatgtgatag ctgctttttg ttcttgattg aagcgattat
     2161 aggaacgttt ctccatcatt ttaaagaaag agctgtttcc ttcactttct tttacttgta
     2221 cttcttcctc aataagtccg ggtgcttcgc cataagccgc ttcataggta gcaatgattt
     2281 cttcttgtag ccgaaagact tgttgaagct ttttatactt gttctcccag cgtttcaatt
     2341 ctttttgtaa tcggagaatt tctttttcat tcaactgatt gatttccttc ccatctttct
     2401 tctttgaatt tgtttatttc aagttggtta tttaaagttt cgttgtctat tttttcttta
     2461 tactaacaga cctttctaaa aatgaaaatg ggaagcgata tgattttagt tttctgaaaa
     2521 tttttccttt ttttcaatcg tttatgctaa gataaggaag acaaaaatat tcataaaagg
     2581 tgatgaaaaa aatgcagact tcctttcacg aggggcgtac aataggactt ttgctgacat
     2641 ttatcggagg agccatggac agttatactt atatccaata tggtgctttt gcctctgctc
     2701 aaactggcaa tatcattcta tcgatcatcc aagcatttga cggagaatgg ctaagtgttg
     2761 gtaaaaaagt attgtctacc cttttctttt tcttagggat tcttctaaca aagtatctaa
     2821 ttgattattt tagaaaaaaa gaaaaacatt tctggcgctt gtttgttcta tattatgaag
     2881 cagtgatttt tttctttgtt agtttgacac ccgtccatga gcatcccgca ttagtaacga
     2941 ttttgattgc ttttactgca tcgatccagt gggtagcttt tgataaaatc aacgggcgtg
     3001 cctacactaa tttatttaca actggaaatt taaaaggggt agcaacgaac ttatatgatt
     3061 attgtatttc taaagaaaaa gcagattttg atcgattttt ccactatcta agtgtcgttc
     3121 ttgcctttat cgcaggagct attgtccttg ttttctgtca tcatctgttt ggcgcccatt
     3181 cgattttgat cgtttccatt ttatttttga tcatggccct cactcagaca tttcatttat
     3241 ggcaatttta tcatacgaat cggttcacta tttgatctca atgaatcaaa aaaactaagc
     3301 aagatgctgc tcacagagaa atatctgcga gtgagcaatt tgcttagttt ttatattatt
     3361 tagttgtaaa gattcccttt gctttcgggt tatttgtagt gtagcggatc gactgggcat
     3421 ctgcctcttc tgtcattttc tttgcccgca tcttgatttc ttgtacgatt tgttctccac
     3481 atgccggatc ggctacgaaa tcatatttgt ccccatcgat cactattttg ggacatatat
     3541 caaactgttc ataccactct tcgtatcgtc gattgagttc tttatagtag gaatagagtt
     3601 caggatcttg ttctagctgt tcatattccc ggcctctttt tttgattctc gctaacatag
     3661 tttcaaagga tacacgaata taaaccagta gatctggttt cttttgtggc gaaatttctt
     3721 ctaactcctt cagcatcgtt tctaaaagac cttcatactg ctttacttct acttcagaaa
     3781 cacgccctaa atctgcattc aaatgaaaca gcatactatc ttcgtagatg gagcggtcta
     3841 gtacgttgtc atcatggctg aaagcatctt tgattgccaa aaagcgtttg ttcaaaaaga
     3901 aaatctgtaa taaaaaagta tacttttctg gattagaata aaatagcggc aatacttcat
     3961 tatcttccac gttctcataa aaaggttttg tttctaaatc ttgggcaagc atttcagtta
     4021 gtgtgctttt ccctgcaccg atcgttcctg caacaatgat cacaattatc ggcatccttc
     4081 ctgtaataaa atctgttctt cattttacta cttcatttat agaaataaaa gccttgactt
     4141 tatctgctcc ttaaaagcta cttctctaaa agtagccaaa agtaactcct attcatgaaa
     4201 ataaaggctg tgacaaaagt cttgtcgcag cccttttttc aaataaacag tattctcctg
     4261 ccaacacctc ggtcacaagg caatattcat aaaacatgaa aagctgtttt ttaacattat
     4321 tccttgtaac tcagagctaa acgggagact cacaacctct ttatgctctc aacattttat
     4381 ctgtttttat tcttctattg tacttgattt gcgtacaact aactgccagt gcagattatg
     4441 agcggtatca ttgtcttcta ctgcgcattt catgacacgt atcatcacac tattttcata
     4501 gattttttct atttttcctt ttattggtga ttcaaataaa tcagatttaa atgcatatgt
     4561 ttcgccgact tcaaacaatt gttcattttc cataagaatc cctccatcac cttttataag
     4621 gtcagtaatt ttttaacata aatcactcga aaaagcacaa acttgtcata aaaagtactt
     4681 ttcgtatcaa aactgtcact ttttttggta aggttcttgg aaaacgaaga tttttttagg
     4741 agatcatgac ggttttgatg tcaaaaccac tttttatgaa acaaaagacg ttttttattt
     4801 tttttagcat aaaatttgtg gtggaggtac gtatgagatg aaagataaag ttgtgaacaa
     4861 attattagaa gccttattca gcggagacaa atcggaagaa gatatggtgt atgtccaagt
     4921 aaaatttgtc ttagaagttt tattgaataa tcttggaaaa ctagcggtcg ttctagtctt
     4981 ttctctggtg acaggctcat gggcggaaac aggaatcacc tttctttctt atatctgcat
     5041 ccgcagatat gcttatggat tgcattccga ctctgaattc gtttgtctgc tgtggacact
     5101 actttattta tggggcgttc ccttggtgat gaaacatctg caattgacta tctccttccc
     5161 aatgatggtc tttttgttaa tcagctgctt tttgttactg cttcgctatg gatcaagagg
     5221 tacagcaatc aatcccattg aacccgagaa acgtccccca ttattaaaga aggcgatcag
     5281 catgtttcta attttttctc tcattacgtt atttttctca gcttcttact tttctaccta
     5341 tttgctactt gggattgttt tagaaatagc tacgttgctt cctattacaa actacttgat
     5401 gaattttgga ggaatctttc atgaaaaaaa caatcattaa acaattaact aacttatctg
     5461 acttttttgt atctggctgc tgctttggta tgagttctga attcaaaatc cctgaaaaat
     5521 taaaaaaata atacgccaat tgtgctataa tttggtaaaa atcaatggga gctgtacttt
     5581 atgtttttat ggtccatagc gttgcaatat ttgatttaca caatttgtat cttattgatt
     5641 gaccatcaca tccgatacaa atatctgttt gcgggagtca ttctgatttc ttgtattttg
     5701 attggatggg tctattcgat tttagggatt tttactgctc taattgctat aggaatgctg
     5761 atgcttactt ttttcttctt ttcgacttcg cgtcaacaat tggtttacgc gcttcctttt
     5821 gggttattga cgtcgctttt aggagatcac ttaacaagca tcggcgattt ttttgttttg
     5881 aaatcttcgg ttattgaacc aggagattca ttgcagtatt ttcacattgt cttatccgct
     5941 tttttatctg ctttattagc tttttgttta aaaaaatcgt tagagacttg gtttcacact
     6001 gtggaccgcc gtatgatcgg tacgatcggg acattgatat tatttacata ctatatcatt
     6061 attttttata cccgtttttc tggtgaaaca cctgatgtct tagctttgaa tacgagtttt
     6121 ttcattctat atctgtgtgt tggcctgatc atcttggttt attattggaa aatctctaat
     6181 aagaaattag ctgatcagtt attagagcaa caagtcagga tgcaacagga ttatatcaaa
     6241 gatctagaaa aaaactatca agacttgaga gaattcaaac atgattacca aaatctgctt
     6301 ttttctcttc acagctatat ttcagaaggc gacttagaag gattacggtc ttattatgat
     6361 caaacgatat tacctacacg ccagatgatg aacctttttc cggccaatct aagtatgttg
     6421 gataatatga aagttcctga gatccgtagt ttactgtcat taaaactcat gatggcacag
     6481 gaaaaagggt tgatcgtcca gcttcatttt cctgagccaa tcgaactgga tacgaaacat
     6541 acggtaaacc ttgtacgtat gcttggtatt atcttggaca atgcgattga aggagctagt
     6601 gctgcaaaga agaaaaaaat cgaattgacc atactcaaga agaatcattc cgtgatgatt
     6661 cgtgtgatca atacaacaac aaatacatta ccgttaaacc agttgaaaca aaaagggttc
     6721 tcaacaaaaa caaatccaaa aaacgaaggc cttggcttgt ttatcttgga tgaattgaca
     6781 aagaccaatc cttatctttt ccttgaaaca tcaattgaaa accagcgttt ttctcaaacg
     6841 atttatatac aaataaaata aaggagtaat gatatgattc ccatttatat ttgtgaagac
     6901 gatccaaaac aattagaagt tttatcaacg gtgatcaaaa atagaatcat gattgaaaac
     6961 ttagacagtt atgtccatat ttctacctct gatccggaag aacttctcaa tgcagccaag
     7021 atcagaactt cttcttttgg tatttacttt ttagatatcg aactagaaga cagtgaaatg
     7081 gatggtgtag cgatcggtaa attgatccgt gaattagatc ctttaggaaa aataatctat
     7141 gtgacttccc atacggaaat gtcgatgaaa atcttaaaaa gcaacatcga accgactgat
     7201 tatatcgtca aagaagatct ttatgattta aaagaaaatg tcgagcatat cctgagcaaa
     7261 attttcgaag attttcaaat gtcccaaatc caaaaagata tcttcaaaat agagtttaat
     7321 gatgagatca aatttttacc gatcaaagac atccaatatt tttctacttc gctaggagcg
     7381 ccacataaac tggaagtcca tttgactcat gcccaattac aattctatga gaagatcaaa
     7441 gaaatcgaga aaatgcatcg gaattttatc cgttgtcata aatcctatgt tatcaataca
     7501 caaaatgtgc ggtcgattaa taaaaaaacc agagaagtca cgttggcaaa cggcgagacc
     7561 atcccagcct ctatccgtgg attaaagaaa ttgatttcat aaaaagaacc gaatagtttg
     7621 gaccgactaa atgaccagcc gatccaaacc cttcggttct tttttgccta tcagtgcaaa
     7681 agcatttcct gtgcaatttc acgtccatcc attgtatctg gataaaatgt tggccaattc
     7741 tccatttctt tcagcaatac ttcttgactt tcatttcctg catataaatg aaaatgttcg
     7801 gagtcagccg gtgcaatcgt gtgatcactg aattgaatat atctaaaagc gccactttcc
     7861 ttatcttctg cttcgaatag ataccttacg ccacggtttc ctgcctcgta ggtcaagatt
     7921 tctttcccta cataacgata atttgaacgt ttcgttgttc cattcttaaa cgtaaatgcc
     7981 atctctttct cactgatctt gattgaagca atatctgttt gatagccctt cgtataatat
     8041 tctttatatt cttgtgctgt cttatcctga ttcaattttt ctttatattc aaaaacagga
     8101 tcaaaagtcc catcttctac atacgtatct actgtttgcc aagttcctgc ccaatctgaa
     8161 agagagcgat cttgtacatc ctcgtcttca aaataaccgt tgtagacgct cttttctttt
     8221 ttgggggtca aagtatcttc tagcgggttt tctgtctctg tcgttttttt caacgcttcg
     8281 aggtttgctt ccatgataga aagatagttt tctccatttt ctatttgttc attcgtcaat
     8341 ccttccaaag gattcaatac ttctaaagat acacctgctt cgtttgctaa cgttctggca
     8401 atactatctt tggcgttttc ttcaaaataa atatagttga ttccatgttc tttaacaaag
     8461 tgtttcagtt cagcaattct cgccgcagac ggttcttcac tagaagaaag accagcaatc
     8521 gatacttgat tcaatccata ttctaacgct agatagttga atgcagcatg ttgcgtgaca
     8581 aaagatcttt gttttgcttg cttaaaggtt tcttgataaa gctgatggag tgcttgaagt
     8641 ttctcggtat aggctgctgc attttttgtc catacttcct gtttttctgg gtaagcttca
     8701 atcagctgat cacgtatgtt tgctacctgc ttgattgcta aacttggagc tagccataca
     8761 tgtgggtcta gctcatgtgt atggccttct tccccgtgtt catggtcatg atcatgtcct
     8821 tcttcacttc ccggaagtaa aagcatacct tctgtcgctt ttatcgtatg gacattgcct
     8881 tcttgtaatg ttttttggat tgcggggacc catgtttcca tgttttcatc attataaaca
     8941 aagatatctg tatcttggat ctttgccatg tctttagcag aaggttcata atcgtgggaa
     9001 tcagtccctg ctggaatcaa taactctact tctccttcat ccccgacgat tgcttttgta
     9061 aaatcataca tgggataaaa ggtcgtcatc acgctcaatt tttctttact gtcctcggct
     9121 ttcccacttg ccccgcaagc agttaacata cctgctaaaa ccagcatggc caatatacct
     9181 atcaatcgtt tcatttatga cactcctctt ctcatcttct ttcatttgaa tgttccgatt
     9241 cgcttttcgc aaaccgtaat agttacgttt tagcaacagg atttaattta tcatatggtt
     9301 aaaagaactg tcaagcgttt tcttatttta attgatgaga aagtttatat gaatgattga
     9361 cgatattttt gcaatcaacc tctatcattt tccttttata cccgcttttg tctataatga
     9421 agttatagat aaaggaggaa aaagagtgaa aaaagaaaca gatacaacga atcgtttgat
     9481 ccgcttttta ggaggaaaaa cctcttacta tgttttagga atagttatct tacttgcgct
     9541 gatcatttat tttttccatc agatctcgtt tatattcaag cctgtcggaa tcattatcgc
     9601 tactatcttg cccccattat tgtttgcact gatcttgtat tatcttttgg aaccagtcat
     9661 caaacgtttg agcaagcacc tttcgaggaa cttgtcagtc attatcgttt atgtgattat
     9721 tttattttta ttggggcttg gaagcatatg gcttattcct tttcttgaag aacaggcaaa
     9781 gacattgatc agtagtttgc cagatttgtt tcaagatttt caagagagtc tccgacaatt
     9841 tttggagaaa acaccttttg ccgattcatt taatcaattt ttcgcttcta ttgacgacgt
     9901 gacgtctgat atagctggat ttattggaaa ttattgggaa agtggcgcac aacggatcag
     9961 caacattttt tcaactgtta cagctatttt tattacttta tttactggtc caattgtcgc
    10021 ttttttcctt ttgagagatc ctcaaaaatt ttatcagtca gttttagcga ttgtcccacc
    10081 agcatttcgt acagatttta aaaatttgac aaagattgct aatcaacaat tgggttcatt
    10141 tctaaagggg caaatcattg cttcatttat ccttggagca gtctactggg tttgtttctt
    10201 actgatcgga ttagaatttg ctagtgtttt agctatttct gctgggttgt tatgtatcat
    10261 tccatatatt gggccgttta tcgccttctt tccaggtttg atcattgcat tccaagactc
    10321 tacttttatg gctgtcaaat ttgtcattgt ctggtttgcc gtacaacttc tccacggtga
    10381 cttggtgatt cctcgagtaa tgggtgatcg tttacagatc catccgatta cgatcttaat
    10441 cgttttactt gtcatgggtg atctaatggg aattgtgggc gttatttttg gtattcctat
    10501 ctatacgtta gtcaaattac tcgttacttt tattttccgt aaattcaaac aacgctacaa
    10561 taaattttat ggcgataaag gagagtatga aaactcttca ttttctgaag aagattattt
    10621 taaataaaac acaaaacgtc gatcttacga gaatagctcg taggaacgac gttttgtgtt
    10681 tttgttttaa tttttcagca aagcaattgc ctggtatggt tttagctcca gactttgatg
    10741 aagaacagta tcggaataat tatcgataag aatattggct tctgtgaatc cttctgggat
    10801 ggcgatcctt gtatctttat catagaaatt tgttaataca agcagctgct gtccttcaaa
    10861 ttggcgaaga aagccataaa cttccggatg atttgcttca taagcatgat aatctccttg
    10921 tgcaacgatt ggatatgttt ttctcagccg aatcaatttc ttgtaatagg acaaaataga
    10981 atcttcagaa gatagttctt tttctacatt aatctcttct agcttgcctc caacttttaa
    11041 ccaaggtttc cctgtagtaa atcctgcttg ttctgtgtct gaccattgca tgggtgttcg
    11101 agagttatcg cgagattttg ctttgatccg acgaaacgct tctttttctg tgaatccttg
    11161 ttcaaggagt aacttataag cattcaaact ttctacgtca atatagtcgg aaatcgatga
    11221 atagtctggg tcgatcatgc cgatttcttc tcccatataa atatagggcg tcccacggtt
    11281 caaatggatc atggttgcta gcatctctgc tccttgtttt cgatactgat cgatagctac
    11341 aaatcgattg agcgcacgtg gctgatcatg attattaaaa aatagagcat tccagccgtt
    11401 accctcactc atcttttctc cccatgtatg aaatagtcgc tttaattctg caaaatcaaa
    11461 tggaggagta gaccatttat tcccattttc ataatcgact ttcaaatgat ggaaattaaa
    11521 gaccattgat aattcttctc tactaggttc tgtatacaag atacaatttt cgatcgtcgt
    11581 agagctcatt tcaccgactg tgatgatagt ggaatccgtc ccaaacgttg cttggttcaa
    11641 ttcttttaga tacgtatggg tgattggttt gtccgtatat tcaggtttgc catcattttc
    11701 tttattattt ttcaattgtt cgtctttacc gataacattg ataacatcga aacggaatcc
    11761 ttttacccct ttttccatcc aaaaacgaac aactttgaat agttcctctc ttactgcttg
    11821 gttccgccag ttcaagtcag cttgtgtccg atcatataag tgcaaataat atttccccgt
    11881 atctccaaat ggcgaccaag cacttccacc aaattttgat acccaatccg ttggctcttc
    11941 tcttaaaatg aagaagtcct gataatgctg gtcccctgct agtgcttttt tgaaccattc
    12001 atgttctgtc gaaacgtgat tcagcaccat atcgagcatg atttctatcc ggtatttctt
    12061 tgcttcggct attagctgtt cgaattcctc catcgtacca aagataggat cgatggctgt
    12121 gtaatcagag atatcataac cgttgtcttt ttgagggctt ggataaatag ggtttagcca
    12181 aatcatgtcg atgcctagat cattcagata cgggagtttt tctttgattc cttttaaatc
    12241 tccaattcca tcaccattac tgtctttgta agatttggga tagatttggt aaatgacttt
    12301 gtctttaaat gtcatggata tttacctcca ttcaactgat ttattaccta ccacaactta
    12361 tatatacaag ttatcattta ttattataac tgaaccgaaa gcgtttgtaa aatttatctt
    12421 tatacattct aagaggtctg ttgcccaata aaaaacaaga gatcagacat gtatttgtct
    12481 atcctcttgt ttaaaatagc aaaatattct tgtttcttct ataaatggtg ttcaaaagct
    12541 tttatctacg gcaatcagtc ttctcaaatg taaaacatga ggagctaatt ttatttgagt
    12601 attctttttg ctcgatgcag gacggctttt ttacaacctc tgattcatgg tgttcaatca
    12661 gctgtccttc ggtattagct caaaaaacgg caaaatatga aaaactattt tgccgtttgt
    12721 ttctctaata ctcaggtcaa accgctgatt tcacaacctc tgtttttatt cattcgattt
    12781 caacgcatca atcatatcga tatgctttaa tttgaaatgg gtaacaatca tcacaataac
    12841 cgtaaatacg atagttaatc ctgctgataa gacataagcc caccatgtga tcactaatgg
    12901 aaagatgaca ttctccatag acgcttgttg caagatgaag tcagtcaata tatatcctac
    12961 accaaatcct ccgatgattc ctaaaagagt aaagatgata ttttctcgaa caatgtacat
    13021 cgtgacttct ttatcaaaaa atccaaggac ttttattgta gataattcgc gtacacgctc
    13081 ggaaatattg atattagtta gattatacaa gacgataaaa gccagcagtc ctgataatac
    13141 gacaaagatt aagacaaccg agtctaagtt cgccattgat tcttcttgcg tttcgatttg
    13201 tttggacatg aaagacgtat ttgtcacagc gtctgtggca agcagtttct cggaaagcat
    13261 ttcttcttct tttgttgtca tcttatctgt acgcagcaaa aatgcattat ccatcatcga
    13321 ttgatcagta actgaagcat aatattcagg ggacatataa ataaagctac ctagatagtt
    13381 tttcacgatt tcttttactt cgactttcca ttctttttga tcttcatcat aaagcgaaat
    13441 atgatctcct gtagataagt caaatagttc tgctactttt tgcgtaatag cgattcctgt
    13501 tttaggcaag gagcgtgggt tctcttcaag atcagttaaa tggataaaac gatccaaatc
    13561 agctgtttta cttggtacga tcactgtcag actttgcgaa gcatgtcctt tttttctcaa
    13621 ttctacggtt tgagtatgaa tagctaggat ctcatttact ttttcttgct tctgcagcat
    13681 atccgctgct ttttgacgac tatcttccgt ggatgaatcc aatgtaacga ttgcttgata
    13741 gtcaatgatc gttccaaatt gtttggctga aacagaactt agagaatctt tcaatcccac
    13801 gcctgcaacc atcagcccgg cacatccagc aattccgata atcgccataa gcattcttga
    13861 cttatatcgg aagagatttc ggtaactgat tttttgatta aagctaaggc gtgaccacag
    13921 cggagtgatg tattcaagaa aaatgcgttt ccctggcttc ggtgctttcg gttgaagcaa
    13981 tgcagcaggc cgttctctta aatctttgaa taaaacgatc attgcagcgc caaatgcggc
    14041 aatgaagaat gcggcagctg cttgtaaaat cggcggccag tcataaaaaa taagcgcact
    14101 cccaatatca taccgctcgt tagacagctc aaaaatgatc cttggcaaaa gattcgttcc
    14161 taatatcgtt cctaaaacaa tacctatccc tgaggctaga cttgcataga ttgcatattt
    14221 ttttgcgatt tcaactttcg tataacctaa cgcctttaat gtcccgattt ccctgcggtt
    14281 ttcttctacc atacgtgtca ttgtcgtaaa ggtgatcaat gcggcaataa agaaaaagaa
    14341 aacaggaaaa acattggcaa tcgcagcgat acgatcagac agatcaccaa attcttgaaa
    14401 accgacattt tctgaacgtt cattgtatag gtatgatggt ttagttaaat tttcaatttt
    14461 ttctgcattt cgattcaatt cttcctctgc agaggccagt tcacgttctt tctcaactaa
    14521 ttgcctttct tgttcagcaa gttgagcttc ttgttgagat aatgtttgaa cagcgattgt
    14581 tcgttgcgct tcaggaagct gctggatagt tgctgactgt tgcgctaact gttttttagc
    14641 atcttctaac cgtgttcttg cttgcgttgt ttgtgctttg ccatccgcta ttttttgctg
    14701 tgccggttct aattcttcgt tggccgattt tttcagctct tctaagcgtt cttcgggtcg
    14761 atcttgtaaa tacttttctg ccttttcttg attttgctcc attttatctt tgtaagcttt
    14821 gctgtacgta ttcaaattct ttacgtttgt aaaatcaaga tataagacag aataaatatc
    14881 tgttttaaaa acggattcag gtaaatagac aaaataatcg acgctaccat ttccgacgtt
    14941 cgcgtatcct ctctccaaat tgttgatgaa caatggagaa cggacaaagc ccgtgatggt
    15001 atactctttt tctttcaatt catctgaatc aatctgataa atatcaccga ttttataccc
    15061 ttcatcttcc gctttttcat ccaatacaag ctggtttggt ttttccggga gttttccatc
    15121 agttaattgt agcttgttct gcttgttatt tttgttataa gaaaatattt gaatcacttc
    15181 gtttttgcca gtattggaat aatagatctg ttttcctgtt tctacttttg catctgggat
    15241 cttttctgct tgagcaatat ctttttgtgt caagccacca gtagaaacga tttgcaaatc
    15301 agaaagtcct gctgtaccaa catagtgatc catcgttttt tgcatgacag gtccagcagt
    15361 tttgacacct acaaataaaa gtgttcctag catgacaata aggatgattg caatgaatcg
    15421 tcctttagat tgccgaattt cgcgaaaacc tgctttaaga tatgttcttg ttttcataaa
    15481 atccccctac catacaatct catcaatgga tgaaggatga tcatttaatt caacagacct
    15541 tactttagca tcattaatgt gaatgactct atcagcgatg ggcgctaaag cagaattatg
    15601 tgtaatgatc aaaacggtat tgccgtgttg tctgctagcg ttttgcaata atttcaatac
    15661 ttgcttgccc gtttcaaaat ccagcgcgcc tgtcggttca tcacataaaa gaagtttggg
    15721 atttttagct agtgcacgag caattgaaac cctctgctgt tcgcctcctg ataattggga
    15781 cggaaagtta ttcaatcgat gagctaggcc cacttgcctc aatacctcta caggatcaag
    15841 cgcatcagga gaaacttccg ttgccaattc cacattttct tttgctgtta gattaggaac
    15901 gagattgtag aattggaaaa cgaaccctac atctgttcgt cggtaagcag taagctgttt
    15961 atctgaaaat tgcgcaatat ctgtgtcatc aataatgatc tgaccttcat caggtgtatc
    16021 catcccacct aaaatattaa gaatcgttga ctttcctgct ccacttggac caagtataat
    16081 cactaattcg cctttttcaa ttgaaaaaga aatatcgtca tttgctgtaa tggtcgtttc
    16141 tcccattttg tatcgtttgt attcattttt tacttcgaca tacttcactc tgattcctcc
    16201 attttcacga tatttctcgc tatatctatg catcgaatgc gttttctcac acttatttat
    16261 tttaccactg aatttctcta gactaaagaa aggaatcctt taatttagtt gacaaataaa
    16321 cgatttatga tttacttaca taaaaaacat tgttatttga aagaaggaac atcatgaatg
    16381 atttaaagga aaatatgatt tttgatttat caaaaactgc ttttgtcgcg atcgacatgc
    16441 aaaaagagat cgtaaataac ggtacactct ctccacacac tgcagcatct attctgacaa
    16501 acaatgattt gttagtaaga acattgaaac gtaccgctgc cttgaaagtt ttagtgaacg
    16561 tagacatctc cactttccct tatttgagtc aacaaactga tatgggtgga atgactgctt
    16621 ctgtacctcc agaattcact gatttacttt tgaaggattc tttgaaagat acagataata
    16681 tgttgactat cacaaaatac aatccaagtg ctttttttgg tacttctctt gatcttcaac
    16741 ttcgccggcg tggaatcgaa actatcattt taagcggtgt agcgacaaca aacggtgtat
    16801 atgctactgc attagatgcc tttcaacacg ggtaccatat cgtgcttgct gaagatgctt
    16861 gcagtgatcg tgacaaagaa tcccatcaat tgttcataaa aaagatattt ccgaaaactg
    16921 cacgagtacg ctcaacgaaa caaataatcg aagctatcca acaaagcaaa taagtactgg
    16981 cacacgtgat tttcatttac ttctttttga tttcatggga aaataaccac aggatttaga
    17041 taggagtatg aacatggtga aaaaaaagat cacaatcaaa gaagtcgcta aactttccgg
    17101 cgtttcaatc acaactgtct ccttgatatt aaatggaaat acggagaaat tcagtccgaa
    17161 aactgttcag aaagttttag ctgccaaaga agaattaggc tatcagcctg actattttgc
    17221 tagacaaatg attaccaagg aaacgaaaac aattggtgta ctcgttcctg atatcacgaa
    17281 tccattcttc agtacactga tgcgaggaat tgaggatatc ttgtataagc agaatttcgt
    17341 cacgatatta tgtaatgctg attctgatca tcaaaaagaa atcgaatatc tagccgagct
    17401 cacacgtcga ggtgtggatg gattcattat cgcgacttct gctgtctcta cagatgcaat
    17461 caacgaaaat ttgaaaaaac aaggccggcc cttcattgtt ttggatcaga aaaaatctga
    17521 aggatttagt gatgccgtac gaacagacga ttttcgcggt ggttacctag cgggcatgca
    17581 cttgcttagt ctaggacatc aaacgatcgc ccttgtctat cctgaaaatc caccggaaaa
    17641 tgtccacgct cggattgagg ggtttaaatc tgctcttgat gtttaccaaa tccctcacga
    17701 ccaactgatc ctcttaccta cacagttctc aaaacaaggt ggctaccaga tcacagcaga
    17761 acttcttgat tctgctgcaa ctggtgtgtt tgctttaaat gatgaattag cttttggatt
    17821 atatcgcgga ttagaggaag ctggaaaaag catccctgaa gactacagca ttattggcta
    17881 tgataatata gatatgtgcg agtatatcaa gccgaaactg acaacgatcg cacaaccaat
    17941 cttcgaattg ggacaaacat ctgccaagtt attgttagat cggatccaat tcccagaaaa
    18001 agaatgggaa gaaaaaagat tacctgtacg attagaaaaa agattttcaa ctgcaccctt
    18061 gaaatagctt tcaaggtgtg ctatattttt atcgaactat taaaacgttt tactaaaacg
    18121 ttttaaaaaa taattttccc atcaaaataa atgagatagt tggaggtttg taaaatgaac
    18181 acggttacag taattggaag tattaatatg gacactaccc ttcgtttaac caacatgccc
    18241 aagccaggcg aaacgatgca tgcgcatgag atttttcacg ctggcggagg taaaggtgcc
    18301 aaccaagcag tggcagccaa acgttcaggc gcacggacat cttttattgg aggcgttggg
    18361 gctgacagcg aaggacaaca gttattagat ctactgacaa aagaaaacat cgatacatct
    18421 gggatagctg aaatccaagg cgccacaacc ggacaagcaa tgattatggt agacgcagca
    18481 ggagaaaata gtatcttgat ccatgcaggg gcaaataatg ctttccatga acaagaagtc
    18541 ctaaaaaaca agcaactgat tacaaacagt gactttatta ttgctcagtt tgaaagtagc
    18601 ttagatgcga cgatcctagc tttttctatt gcaaaagacg caggaaaaac aacgatcttg
    18661 aatcctgcac cagctcgtga aacgattcct actgaactac ttgaaaagac agatatcatt
    18721 ataccaaatg aaacagaaac agaaatcatt acaggaatca gagtgacaga tcataacagt
    18781 ttggtcgcag cagccgaaaa actccatgaa ttaggtattg gaacagtaat cattacacta
    18841 ggcagtgccg gcgctttcta tcatacagaa aaagaacacg gtatcgtccc tgcttttaaa
    18901 gtggatgccg ttgacacaac agccgcaggt gatacattta ttggcgcatt aagcagcaca
    18961 cttcagccag accttagtaa cttgaaagaa gcgatccttt atggaaatct agcctcttct
    19021 gttgctgtgc agtcttatgg tgcgcaacct tctatccctt accgcgaagc tttagaacaa
    19081 tcagcagtct aaaattaaca atgagaggaa gaaacaaaca tgaaaaaagg aaaagtaatc
    19141 aattcagata tttcacgagt aatcgcacaa atggggcact ttgataaatt aagtatcggg
    19201 gatgcaggca tgcctgtccc ctttggtact gaaaaaatag atttggctgt ctcaaacggt
    19261 cttccaagtt ttttatctgt tttagaaaac gttttagaag aacttgaggt acaatcgatt
    19321 gaattggcgg aagaaataaa gacaatgaac ccggaaatat tggaaaaaat caaattattg
    19381 ttaccagata caccgattag ctttatccca catgaagaaa tgaaaaaaga gttgaataac
    19441 tgtcatgcat ttattcgtac aggcgaaatg acaccttacg ccaatattat attagtgagt
    19501 ggagtggtct tctaatggaa acgacaatga atcctgaaca gatggtactg tccgagcaga
    19561 ttcatatgct gattgaacag ttgggaggaa aagacaatat tgattctgtc cttccatgtg
    19621 cgactcggct tcgtatcaaa ttgaaaaaac agccagtagc caatgcagaa aaattaaaac
    19681 agcaaatccc ttccattaag tatttcattt tactcgatag atatgcagaa ataatcatgg
    19741 atcagggatt ggctcctgct tacaaggaat tgacgcgtca aatcgatcat tcagctccga
    19801 aagaaaagct aaatccgatc gaaaaactat ttggtttgat atcggaaatc tttacgccga
    19861 tcattccgct gtttgccggt tctggtatat taaaaggtct ggttgtttta gcgactacga
    19921 ttggctggct tagtgaaaca agcggaactt atcatatttt gtctgctgcg tcgaatgccg
    19981 tcttttattt tctgccaatc tttcttgctt tttctgctgc caaaacgttt aaagtgaatg
    20041 gatatattgc ggctgtcatt gctgcagcac tgattgaacc gaatctgacc tctttacttt
    20101 cagacagtgg caaagcagct actgattttc tggggattcc tgtagtactg atgcagtata
    20161 cgtctactgt catgccggcg attttaggta ttttctttta ttcttttgtt gaaaaattcc
    20221 tcaaaaaata tattcgcgaa aatatgcaat tagtatttgt acctttactt tcattagtta
    20281 tcacggttcc tttgacgtta atggtattcg ggcctgcggg tgtttacctt ggcgaaggtt
    20341 tagctgccgc aattacttgg atgatggata ttaacggacc gatcagtggc gcaatcatcg
    20401 ctggtggttg gaatattttg gtaatatttg gtatccaatg ggctgtaaac cctgtcatga
    20461 tcagtaatat ctctgcttat gggttcgacc gaatcgttcc gttgacagga gcagccaatt
    20521 ttggaatggc tggtgcagct ttaggtgtat ttctgcgaag taaacgttca aaaacccgtt
    20581 cgatctccgg ttctgctttt gcttcgattt tacttgcggg agtgaccgaa ccaactgttt
    20641 acggaattgc gatcccattg aaaaaaccat ttgttgctgc ttgcattggt gcagctgctg
    20701 gtggagctgt aatgggattt gcccaagtga aagcgatcgc ttttgtattt ggtagtctga
    20761 ccacattgcc tgcattcatt tccggtactt ttttctggta cttagcagga ctggctgtta
    20821 gtctagtcgt agcaatgatc actactctcg ttagcgggtt tgatgaagac ctaatgtctt
    20881 atgaatagtt aatgaaaaag actgaagtat gagatgttta tcacatattt cagtcttttt
    20941 ctttttagga tcattatttt tataaatttt tgtgatactt tccaaacttt ggtataattt
    21001 acatatgcat aaccattttt atccccactt gtttatcccc aaacaaaggt ggtctatgca
    21061 ataaaatgaa cgtcaaagaa aaacgaatga atgctcgttg cttatttaca aattgtttta
    21121 tttagaatac acagacagat ttatgaaaag aaaaagcttt tcgacacgat tatctgttcc
    21181 aaaaaatgtc taatgaatgc tgaataagac agaaaatcaa tgaaaggaaa aggactagtt
    21241 tattatgaaa aataaaaaaa cgatacgttt gctagctgct acttggctga ttgctccgtc
    21301 tgttttgtcg accaataccg tatttgccgt agataatcaa atagctgatc aaacagtcac
    21361 aacagatacg actacatcca gctcagctac tagtgaaaca acggattctt ttacagaaga
    21421 cccaactgga acaactaact caacaacagg atcgaccgag ccaactgaaa ttgatccggc
    21481 tgatccatca gaaaatgaaa agccagccga gacatattcc cttacaattg ctccttcttt
    21541 gattggtaag atccaactga ctgcttcgac agcggattcc cctgaacagc aacctttgga
    21601 tattacggaa agtggaacgg tagaaggttt attagcggaa acgaaggtaa cttacactat
    21661 tacgccagct gaaggtgaac aaattgcttc agtcgctgtt aatggtgcca aagaagtaaa
    21721 ttatgatgca aagaccttta cagtaggtac gaacaattta tctattactg cagaatttaa
    21781 agctggaccg gatacttcaa cagagaaacc aactgatcct agtgaagaag tccctagtac
    21841 accagatccg gaaacgccaa acaaaccaga tgtgggtaat aacaacaata ataatggttc
    21901 aaataattca aacaataata acaataattc gaacagcggg aattcttcga atcaaacaaa
    21961 cccatctgga tcgaataaca atggaaatac tcagagagaa ccaaaccatc caaacaatgg
    22021 ttcatcgaat ggcggaacga acaatgcttc atctcgtcca aatacagcaa atcaaagcgg
    22081 tatagagaat ccatcttctg cttcttctga ttttgttgta aaatcaccaa ttgatgctgt
    22141 attgcctaca aatacaacga atgtccaaca agcaatcgtt cgagaagcat tcaagcatct
    22201 tggaaaacca tatgtatggg gagcaaaagg tccaaatact tttgactgtt ctggtttgac
    22261 ttattacgta tatatgaaag caaccggtca ttatatcggt ggctggacag gcgaacagca
    22321 atatgcaggt acgcagattc cagtaagtca ggcccaacct ggcgacctcg tcttctgggg
    22381 accttcttcc ggtgttactc accatgtagg tatctatatt gggaacggac aatttatcca
    22441 tgcgcctcaa ccaggagata aagttcgagt aacttctatt tcagatttca cgcctgattt
    22501 tgctgtccga gtaaacttag caggtttacc tgctgcttca ggttcgctga caggcggctc
    22561 atctatttta gatggattag atggctcgtt ccatttcagt caaaaccaaa caactgatca
    22621 gttcttgaaa aagattgcag atgatgcaca ggaaatcggg caaaaagaag gaatctacgc
    22681 atcagtcatg atggctcaag ctatccttga aagtggatct ggaaacagct tattatcaag
    22741 cgaaccaaat cataaccttt ttggtatcaa aggatcatat aaaggaagca gcgtgacctt
    22801 caatacactt gaacaagata gttccggaca aagctaccaa atccgcgcac aattccgtaa
    22861 atatccttct tacaaggaaa gtttggaaga ctatgccgac ttgatcaaaa atggactgac
    22921 tggcaatcct gatttctaca aaccaacttg gaagtcggaa acaaaagact acaaagaagc
    22981 aaccaaatat ttggaaggac gttatgctac agatcgccaa tattcacaaa aattgaacgc
    23041 gatcatcgaa gcgtacgacc tgacaaaata tgatgaaccg aaaaaagaag cagcaacaac
    23101 tgaagaagca aacgctgaaa tcctcaatat gtctaagcga ttcgatgtgc caatcaaatg
    23161 gacaacagat ggcgtgccaa tcacaggatt agaattttca acgataaaac gcgtcaacaa
    23221 cgcttcttcc atcttccgtg tcgcttcaat ctgggatttg tggaacaatt ttacggcaag
    23281 acagatacca gaaacaacta ctcgtacagt taaagctgca actgttcctg tactaacctt
    23341 gttaagttta gacaaatcat tctggcttga tctgccttta aagaaataga ataaattaaa
    23401 aaggggatgt gacactgtca cactcccttt ttaatttatt ctatttcttt atatttattt
    23461 tgtattccgt tacaaactcg cgtacttggt taggttccag agtgacatct ccaaaacttt
    23521 caagtcgctc cgctcctggc agttcttgag tctcaaatgt gataccgcca tgatttacta
    23581 atttattccc gcgcatttcc ggtccatctt ctccaaaatt tgctgtaaaa atcacgattc
    23641 ctgaagcatc tgtttttact tggatcgata ctttttcatc tgggcttgtc aattccgcag
    23701 catttagtaa accagattgt tttttcaaca aaaatggatg atcgatgccg cccactatca
    23761 ttgattggcg aaaatcactg gcaaagacct cacctaattt tttaggacgt tggaagtcaa
    23821 atgctgtgcc acttactgaa actacttctc ctgtaggcgt tgtatcttta ttgagcgtag
    23881 caaataatcg gctattcaac caaagtatat gttcgtcgat tgactgtgct ggatcaccgg
    23941 ttaaattgaa atagacatga tttgtaggat tgaatactgt tttgcggtca ctgatccctt
    24001 ggaccgtcac tcgccatgta tcatcgttcg tcagcgtata ccgtacttct acatctaaag
    24061 ttccaggaaa tccgttttcc atatctgggc tagtctgata gaaaatgacc gatgcttcgt
    24121 tttcaccatt gatgactgta taatgccact gcttggcttc aaagctaggt gcaccaccat
    24181 gtaaacaatg accagtgtcc gggtctgttt ttacttcgaa cgtttcatca tctaatttga
    24241 atgtgccatt cgtaatccgt ccagccatac gtccgattga agcaccgata taagggtctt
    24301 tttctagata ttcttttgct tcatcaaaac ctaagatcaa ctctcgtttt tcattatcga
    24361 ttattgtttg aaaatgaaca acccgtgccc caagatcact gacagacaaa gatgtcccat
    24421 tttgattcgt gatcgtaatc aagtgatagt tttcgccaaa attttgttca ttgatttcca
    24481 tgttccttgc accctctttt ctttcatttg cactgattcc agcatacatg gaaaccgttt
    24541 tcttaacagc ttattccgtg agtaacgaga aaaaatttac tatattctaa taaaagaccg
    24601 tgaaaaaaag gttgtgacaa aaatattgtc acaacctctt tttccgaaca aacggtgttc
    24661 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagga gctatttttg
    24721 cttggctgtt cttattactt gatgcaggac agctttttca caatctcttt ttcaatacga
    24781 ttccagtata taaccatgtc gtcagtacat agtagatgaa atacaatacg aagaatatcg
    24841 taaacggaac ccaaatatct ttgtatggat tttgcatgaa tagaccaaac atttgtaatc
    24901 cgaataagac atggactaca cctaagattc ctggtgctaa gaataacacg cctacttctc
    24961 tacggatcga ttgtttaagc aacccgcgtc gtgttcctat cttttcaagc atttcataac
    25021 gagcaatgtc actcttgctt cctgacaaga tcttgaacat caaacagcta gcaagcatgg
    25081 tcaagaaagc taaacctagg aagaatccca taaattcaaa accagagaat agaccattat
    25141 acacttcata catttcatat ttctggttgc tgtactctac tttttgcaaa gatggattgt
    25201 tcattttatt ttgagcaaca agtgtttcga ttggtttcag gtttgcttgg aagtctttta
    25261 cctgaactag ctgtaaagtg gattgtggca agttcagttg atcaaattct gattgactga
    25321 ctaccttgat ttctttttct tgctgttccg gcaattcata gcttcgaagt tcctcttgta
    25381 cagtcagttc attcattttt ttagcaggaa ctttttcttt cgtgatcttc ggcggtgttc
    25441 catcttcatg ttttatcatg atcaatggtt gagccgcaaa ttcacttgca ttatagtaaa
    25501 tcgttcccgc atcttctttt tgagtataag aagcagaaag tgttggtgaa agttctttga
    25561 ttttttgctg atcttctgcc cgaggattat tcatcactag atcatatcca gaattgtttt
    25621 ctgtcaagtc tgcaatttca tttttaaaac ctaatcctac cgtcaaagcg cccagtgcca
    25681 aggcaaacag catagcgacc attgagagca tttgtgtata ttcacgaata cgaaagttta
    25741 actgtgacaa tgtgaaattg ttcaattttt tcaatgaaat ctgttctgat ttctttagta
    25801 atgataaaaa gaaaatgatc acagaatgga aaatgaaata agttcctaat acgatcgtaa
    25861 ctaaggctac ccctatacca aagacttgat aggtccccac gtgtttcatc atatagtaac
    25921 cgcagcctag aaaagcaatt cccagtacta cttcaaggaa aaacaagaat ttatgctgct
    25981 tgattcttgt tggtgtctga tccgcacgaa tcaatgctaa aatcggtttt ttgacaatcg
    26041 aaaaagcatt gacaacagca gctaataaaa acaaaacagc aaaaaacaaa acagtgatca
    26101 acaacccgtg aatactaaaa ggtgtaaaat gcgaaatatt gagatctaac tgatccacta
    26161 ataatcggtg gacgatactt gtcaatccta caccgacagc tgaacccact aaagtagctg
    26221 cgatccctac taagaatgtt tcagaaaaaa tcatctgtgc gatttttctg ccttttgctc
    26281 ccagcatcat aaacattgca tagtcttttt gccgcatcgt catcaaaaag gaatttgcat
    26341 ataaaatata aacgaatgtg atgatcccta gtaaaacaga accgaatcgg aaaatgataa
    26401 ctgtagccgc aacgatcgta ttacttttca aaaaagcttc gttgcttgcc attgattcaa
    26461 acatgtaaaa aatagcagat gccataacta atcctgagaa taacaccaag tagtctctta
    26521 aacggctctt gatcccagta agtgataatt tccaaagcat agtcaatctc cccctactgt
    26581 tccaagtttc caagaataga tagtatttct cgatagaagg aatcacggct ttgatctcca
    26641 cgtttcactt cttgatggat accgccatcc ttgatgaata aaatacgttg gcaataactt
    26701 gcagaaaacg gatcatgcgt cacaagaaga atcgatacat gatcttttgt attcaattcg
    26761 tccatcgtat ctaaaagatc ccgggcactt ttggaatcca gcgcacctgt aggctcatcc
    26821 cctaatagaa tcgttggttg tgtgatcaat gcacgtgcgg cggcgacccg ttgtttttgt
    26881 ccaccggaaa tctcagaagg atagctttcc aaaatatgag agatggataa acgttctgca
    26941 attttttgga cacgttgctt gatttctttt ggtctgacgc cttgtaaaga aagaggaaca
    27001 gcgatatttt cttgtgctgt caagctttct aataaattga aatcttggaa aataaagcca
    27061 atctcattcg cacgaaaatc tgctagttga tttcctttta atgtggtcac atctttttga
    27121 ttgattcgga tatgcccatc tgttggttta tctaatgtgg ataaaatatt caaaagtgtc
    27181 gacttaccag aaccagaagc gcccatgatc ccaataaatt cgcctttttc tacatcgaag
    27241 ctgatgccgc ttaatgcttg cgtctttttc tcattgtttt ttccgtatac ttttgttaca
    27301 ttctgtactt cgactatttt gttcatttct ttccctaccc tttaattagt ttttccagtg
    27361 ctgttttcgg cacgtctttt ttttcgactt cttcatacga agtgacacct ttatttttgt
    27421 tccaggtgat ttttaaatat gctcctttac gaagcggtcg agtcaattgt cccttgaagt
    27481 ccagttcttt ttccttaccc tctttcgtat agccaggcag gatatatcta tagttgactg
    27541 tttcttgtcc agagtctgat tttgaaactg tttttgtacc atctgtcgta atttgtacat
    27601 agtattcttc tccacccata actacgtaat ctgcgatttt cacccctgca agtaccacaa
    27661 cgatgaaaac aagtagaccg atcagaagtt ttttcacttt ttccccttct ctcttacttt
    27721 atatactgat tatagaaaaa agcgaacaat tctgccttcg aatcaactat cattcttctt
    27781 tcaattttgt cattttcttt aaaacaacta aaaaaagagt tcaagacaat aatccgtcct
    27841 aaactccttc ttcctatctt acaaaaagta tgtcacttac ggtctttcat gtcgcctgta
    27901 ttgataacag gttgcgttcc tttgtctgta atgattgaga caagtaaatt attgatcaat
    27961 tggactttcc gatcatcggt gaaattgatt tcttgtccat cttcgatttg ctctaaggcc
    28021 atttgagtga ttgtgacagc tccttcaaca atggtttgtc gagcagacaa aatggctttt
    28081 gcttgttgac gttggagcat ggcactagcg atttctgttg cataggctaa atggttcaag
    28141 cgtgtttcga ttacttcgac acctgctact gctaaacgct cttgcagctc tttttgcaac
    28201 tcgtcagaca ctgccgtcgt gtttcctcga agcgtcaaat cattctcatt gaatgtatca
    28261 tacgggtatt ggctagcgat atgccgaatg gctgtctcac tttggatttc tacaaaatct
    28321 tgataatacg ctacatcgaa taatgccttt gctgtatcaa cgactttaaa gactactact
    28381 gctgagattt cgatggggtt tccatccaaa tcattgactt ttaaaacaga actattgaaa
    28441 ttccgaactt tcaacgaaac agtcattttt tgtgccaaag gtatcgtaag gaaaaagcca
    28501 ttttctcgaa tcgtacctaa atattgtcca aaaaacaaaa ttacttttgc ttgattagga
    28561 ctgactacag ttgccgaact taacaacaat aacgtgatca accaaagaaa aatacttaaa
    28621 aaaagtgccc aaactttcgc ttgccacata cctaaataga agaaaaacaa tccaactaaa
    28681 gctaatacga ccaatccgac aatccctgca taaccattga tataaaatgc tcttttttct
    28741 ctcatgctac ttcctgcgct cctttatcct tcaataaata tctaatgtta cttttttaat
    28801 ttttttttcg aatacgcgcc attacacctt ctagttctaa ctctttgtct tcagtgatgt
    28861 accaaccgct tgcctgtgtc cctccggctg cctgcggtaa ataatccaat tttgttcgaa
    28921 cccagtgctt ttcttctgta aaatagtctt ctaccaagac ctctagatcc atccctgctg
    28981 aaagaggcaa atcttcccga acagatttaa agataaatcg ttccttcttc ccatcatact
    29041 tcaatattcc tgtatcgata atcggtaaag aataataatc cagctctttc ttcaccgtca
    29101 ataaatgttt gaaaacttca tggtacactt gctctaattg gcgaccttct gaattgttcg
    29161 ggtaattaac catcactttt tctttgtctg gatcatagaa ggtttggctt agttgagtga
    29221 tataagtatg taatctttcc atttctgttt ttaacttttc tctttgacct tcttctacct
    29281 tcatatactc tttcaccctg ttttcttttt agatattttc tttcttattt tactatagtt
    29341 atgtgcgtta agcgatgaaa aacagcaaag attggaagga tctttgctgt tttttcttaa
    29401 aatatccagt tgagtacagg atcaatctgt ttcattgtcc tcttgatccg tcgtaatatt
    29461 gatcgatgtg tctttagcag tgatctttag ctttttctgt ccttgttcta acaaagtatt
    29521 tgttattggc tggtagtctt tttcaatata agattcacca gacgtttgga tagtatagcc
    29581 actgttttcc atctcatgca aagataaatt gccaccgttt aggatgactt ttccttcttt
    29641 gctgatcaaa ctgtcttcta gatggaagtt gctgttttca cctgtgacga tcaagttatc
    29701 cacattggcc tcatctaatc gaagtgagcc taattcatct ttcgcagtta ttgttcctcc
    29761 tgtaaattca ttgaacgata cccagccatc atgtgtctta gtattcactt ttttgattga
    29821 tacttcgttg atatcaattg cactattatt cccttcgatt gtcaattcag ataattcatg
    29881 atctgcaggg acaacaatag tgatttgtgc acttgtgtct tctaaactaa tgattgcacc
    29941 tgttgatttt ttgactgcat tcacagtcaa cacgccattt ttctccttta tttctggctt
    30001 ttctgtctta tcataagtcg tttttatctc aaaatccaat ccgcgtatga ccgaaactgt
    30061 ttgattttct gcatggatga cgattttgtt tgcatccttg attttttttg tctcagtaac
    30121 catatcgatc aaccgaggac cattatccca agtcagactc ttttgagcgc ctaatgtata
    30181 agttgcacct gctaggaggc caccagtaag catcagacca ataccggtaa tcaaaaaatg
    30241 tttcaatctc aatttatgtg tcctccttca aacgcccgct gaatgcagat ctctttttgt
    30301 aataaaacgt cgtcccatcc atttaacaaa gttcatcatg acataaaaaa gcccttttgt
    30361 tcctttcagc aacagtggcg aaataaatac tgcgcttcct gtggcgaaca gaccgacacc
    30421 gataaagaat aatccactca gaatcgattg tccaaacaca ctgattcctg agatgatcat
    30481 gaataggcca acacctaaga tactgataca taaaacatag atactaaaaa tgagcgctag
    30541 taaagcaacc aatagtcccc caataaccaa tataaacccc agtgctagtg gaatgaggac
    30601 aggagaagca aataaaccga taatgattaa ccaaatcagg cgaatttgtc tttttggatt
    30661 tttcactaac tcgttttctg cttgttcttc ttctgaaaag taactgattt ttaattttaa
    30721 ggcaaactgc tttggtgtac catattcatc aatgatctga tctaatgttt ttccagaatc
    30781 taaaaaatat tcctcataat actggatcaa atcaaaacga tcgtcttcag gaatttcttg
    30841 aagatgatcc acaacttcat tcaaatattc attcatttgt gctctcctcc aataaccggt
    30901 ctaaagaatc tcgaaaaaca atccattcat caataatttc ttctaaatga cgacttccca
    30961 ttggtgtcaa cgagtaatat cgacggtttc tgccttcaaa aggttcatca taggtctcaa
    31021 gatatccttc ttttttcaat cttctcaaaa ccggatatag tgtagattca ctgacagaga
    31081 gactttcttg tacgattttt gttaaggcat acccatacat atcttccttg cttaatgctg
    31141 ctagcacagt accatcaaga agaacagttg gaatctggat gtgcattacg tcaccttcta
    31201 ttctcaagtg taatatttta tttcaatcta tattatacaa catataatat agtttcaaaa
    31261 tcagtttgtc aaatacaaaa aaactgacta aaagatttga tcctttttag tcagcaaaaa
    31321 atggcttctc tttgattttt attataacag tggcataatc caatttgtcc acatccagct
    31381 cagtggcatg ataacgatca ttgcaggtat catgtcagct actgggaaca ttttcacgtt
    31441 gatcattcta aaaccggtag cgaccatcaa gaatccgccg caagctttaa aatctaagat
    31501 catgtctggt gtagtcaatg gataaataaa ttttgccaat aagaacaaaa tataaaaaat
    31561 aatgaattgg ggaattgcta tcacagaaac gacataccct aaattacacg caaaaatagc
    31621 tgctgtgaaa aaatcaagga cagatttaga aatcaaaatc gtactgtctc ctgtcatccc
    31681 agaatctaaa cttccataga tccccgtacc acttgcacaa aatagaacaa tgaccgtcac
    31741 taatgtgctg ataaattcat cgtgggtcat accaagcttt tcactaggga agattttaga
    31801 aataggtact tgcatcagcg cagccccttt atttatccca ttacctaaat gaacaatcaa
    31861 accaatcgct gttcctatca ctaaagcaaa aataacagcc ggcatatttt tcatcggcgc
    31921 aatggaatag attcccatcc ccatcgaaca cacaccaaaa accatcgtca gctgtgcttt
    31981 aaaatgttca cttaatttat ttcctactaa tccgccaatt agtccgccaa acaaaacgga
    32041 acctacattg atcaaaactc cagttggcat caaaaaactc ctctcttttt ctacgaagcg
    32101 aaaaaaatca gaaaaatttc tgtaattatt gctttcgctg caagaaagat tgtaacagac
    32161 agagaagatt acaccaattc attttttgtg ttaaagtatt ctttattgga ataagtagga
    32221 ggaatatgat ggatatccat aaattcgaag tttttcttga tttagccgaa acaatgaatt
    32281 tcaccaaaac tgctgatcga caattcacca cccaaggaaa tatttctaaa cagattcttt
    32341 cgttagaaac agaattagac gtcaaattat tcgaacgcgc ccatagaaaa atcgaattga
    32401 ccgaagctgg aagcttattg ctcccatatg taaaaaatgt cgtagaacaa taccatgcta
    32461 tgcaagaagt gcttgaaacc tatacgaaag acaacaatct ttcattaaat atcttaacga
    32521 taccgacaat gatcaattat aaaggttttt ccaaaatcac cgagtttttg aaacgccatc
    32581 ctgaatttac agtccagttg aaagaagtgg aaagtgtcga attaacttac aatgacgcca
    32641 atgaacatag tgacacgatc cactttgcta gaagtttcca ggccccacca agttcgattg
    32701 agtggttgcc tactgaaaca gacgattttg tagcagttct acctaaaaat catcctttag
    32761 ctagtaaaaa gaaattggat ctaagcgaac tgaaaaagga gaactttttg ctccttggcc
    32821 ccactacgaa cttgtatcaa cctgtactag atctgtgtcg cgaagctgga tttgaaccaa
    32881 aaatctctta taaaggagca agaatcgact tgatcatcaa tatgattgca aatgacatgg
    32941 ggattgctat tgttatggaa aaaacagtca agaatctatt aaaagaaaat actgtgattc
    33001 tccctatttc tccaacaaca gagagttatc ttgcatttac tcgtaaagtc ggtgagcatt
    33061 ctttggcttc agatacattt tgggagtatt taaatcaaga agaaaactaa gccctacatt
    33121 ttctgtccat ctatccaccc aaaaaaagag agcgacgcat gactttcgct ctctttcttg
    33181 catcatgtcc ttgtttcaat gacacctcta acatttctcg tagttcaata agaaagcagc
    33241 gatttttatg ccaaaagaac cgtaagaaca tttccctaat cacaaaaaaa cgactaaaaa
    33301 cccctaaaaa ccttcaatag aatttttagg ggttttattc tttttcctat tgtttactta
    33361 accgtatcat tgctgtcgtt cctactcctt cgatactgct ataatcaata cgtccatcat
    33421 gcagttctag aattttttta accagtgaaa gtcctagccc attcccagaa atttgacgac
    33481 ttgtatctcc ttgataaaat ttttcaaaca ttcgatctct ggtctcttca ttcattccta
    33541 cacctgaatc ggtcacttcc agtacaatct ctgtggctgt ctcaaatagt ttgatatgaa
    33601 tctgtccatt gatttgattg tactttatgg cgttatccat gatattcagc cacacttggt
    33661 acaaaaactc ttcattgcct gtgtaattta ctgcagcaag ttcgatatcc aattctagct
    33721 gttctttctc ccatttaggc tgaaaaaaaa cgattacttc tctgatttgt tcatctaatc
    33781 gatactcttt cttttccagt tggatacgct ggttttccag tttgttcaat tttaagatgt
    33841 tctctgttaa ttgtgaaagc tgcgtgatac tttcgataat ccgatataaa aatatttggc
    33901 gttcttcgtc agagagattt ggggcttgta acaattgaac atatccttgg attgttgcta
    33961 gtggtgtttt gaattcatgg gatacactgg acacaaagtc attcctcagt gtctcgatac
    34021 tattgagttc ttggaccata acgttgaaat ctttatacag ttgctgtact tctgctactt
    34081 tctgctgttc atccattcga atcgaaaaat ctcccgttgc taccagactc atatttgtcc
    34141 ctagcgcact gattggatgc aggatacgcc gcccaacaaa aatcgatatt cctgtaccca
    34201 cgaccatact gaaccccgca aagacgaata acgggtataa cgttggtttt ccagccgatt
    34261 cagcaggcga gatagaatgt cgagacaata agaaaataaa accgagaaaa cacagcagtg
    34321 tcactaagat cgaaatgaag acaatactag caaaatagat ccataattgt gaaaatagct
    34381 tttttttcat ctttaaataa ccgccttata gcctagtccc cgtactgtga tgatttgaaa
    34441 atcttccgtc ttttcaaaac gggtccttaa acgtttgata tgtacatcga tcgttcgttc
    34501 ttctgtattc gtatctaatc cccaaatatc atccatcaac tgttggcgag tgaagatctt
    34561 gttgggataa gacagtaatt tatacagaag cagaaattct ttttgaggca gaatcaacga
    34621 atgctgatct tgtaacactg tataagcatc tagctctaat tttgtttgtc cgattatcaa
    34681 gctactgctg taattgatct ttgaacgtcg aagcaatgct tccacacgta ggatcatctc
    34741 attgacatcg atgggttttg tcatataatc atctgctcct aatttgaacc cgcgtttctt
    34801 atcctcaaaa tcggcctttg ccgtaatcat tagaacaggt gtgtcaaatc tggtgctacg
    34861 taatgagtct agcaagtcat agccatccat tttcggcatc atgatatctg taatgattaa
    34921 ttctatacgt gtcgcttcta gttttttcag cgcatcctct ccattcaatg cggtgatgac
    34981 tgaaaagccc gctcgttcca acacagaacg gtaaagcaac tgtaattgcg gatcatcttc
    35041 tactactaaa atacgattca ttgatctccc tctgtttttt gaaaaatttc tagatttttt
    35101 cttgtagttc atcttcagtt aacatttatt tcgtatacta cgatcattgt tctgatatac
    35161 ttattagtat accgaatatt tcaatacggg agaagggtga ttatgcttca attaaaagat
    35221 ataaaaaaat attataaagt cggcgaaacc accaccaaag cattggatgg tgtgtctgtc
    35281 gctttcagac aaaaagaatt cgtagcgatt ttaggtccta gtggttctgg taaaacgact
    35341 atgctgaatg tgattggtgg actagataat tatgattccg gtgatatggt aatcaacgga
    35401 aagtctacta aagactttaa ggacagtgac tgggatgcct accgaaacaa ctctatcggt
    35461 ttcatttttc aaagttacaa cttgattggt catctaggca ttattgaaaa cgttgaactt
    35521 gggatgacat taagtggtgt ttctaaagac gaaaaacgaa aacgggcaga agatgcattg
    35581 caccgagtcg gcttgacaaa tcatatgcat aaaaagccaa atcagttatc tggtggacaa
    35641 atgcagcgag tggccatcgc tcgagcacta gccaatgacc cagatatcct tttgtgcgat
    35701 gaaccaaccg gagcgttaga tacagaaaca agtatccaga tcatggaatt gatccaagag
    35761 ttatcgaaag aaaaattagt gatcatggtc acacataatc ctgaactggc ccatcaatac
    35821 gcagatcgaa tcattgaatt ttctgatggt aagatcttaa cagattccca tcctcacatc
    35881 gaacgaccaa aagatgatca atttaactta cgcagaacaa agatgagttt ttggacagcg
    35941 ttaaagcttt ccttcaacaa tatccgaaca aaaaaaggac gtactttctt gacctccttt
    36001 gcttccagta ttgggatcat cggaatcgcg atcgttctgt ccctttcttc tggattccaa
    36061 aaacaaatcg ataacacgca agctgaaacg atggcgaaat ttccgattac tatttctaag
    36121 gtaacgacta accagactcg tgacgatgcc ggattgggtg caagcaaagc ggattatcca
    36181 gacagcaaaa cgattactgc aaaagtcagc gaagaagata aagcacagca cacgaataag
    36241 attgatcaaa catacgtcga ttacgtaaca gacatcgatc ctaacttaag taataatatc
    36301 ggatttaccc gtactacagg aatcaatctt ttacgtgatg taaatggcaa agtacagcct
    36361 gtcagcttct cgaatcaaaa tcctgatacg gaatctcttt ctttatctag tacgatgtca
    36421 gcaatgacag gtgtgggagt gtcaagtttc cctacacagc ttgacactag caaagaaaac
    36481 tttttaaaag acaattattc tttacttgcc ggaagttatc cagcctcagc aacagatgtc
    36541 gtgctgatcg tagacggcaa taataatact aatatcaacg cattgaagaa tcttggattt
    36601 gatgtgaaag aagacgaaaa acttgatttt gatgatatcg ttggtacaac attcaaatta
    36661 gtaaacaaca atacttacta tacaaaactt ccaactggga attttatccc taatacagat
    36721 tatgatgcga tgtatcagaa tgcttctgat gaattgaaga tctcggggat cctacgcgtg
    36781 aaatcttctt ctacaatgaa tctcttatct ccaggaatcg cttatagcga tcaattaacg
    36841 actcaaatcg tcaacgaaaa caaagaatca gaaatcgtca aagctcaaag agacagtggt
    36901 gtaaatgtct taactactga aaaagttgac gaaaatgcca aacaaacatt gcttagttat
    36961 ttaggtggag acagcttgcc ttcaagtatc atgatttatc cgaacaattt tgaagacaaa
    37021 gaaaagatct tggattatct agatgattac aataaaggca aatcagatga agataaaatc
    37081 atttatactg atttagctgg aacgatgact gaactgactg gcggattgat ggatgctatc
    37141 acttatgtcc ttattgcttt tgcagggatc tctttagtaa caagtatgat catgatcagt
    37201 atcattactt atacatctgt gattgaacgg acaaaagaaa tcggtgtact aaaagcttta
    37261 ggtgcacgta aaaaagacat cactcgcgta tttgacgcag aaacgtgtat tttaggtatt
    37321 tcttctggga tattaggtgt gtttattgca tggctagcta cgttccctat caatagcatc
    37381 ttatacaaca tgacggatct aaaaaatgtt gctcaactaa atcctgtcca tgcgattatt
    37441 ctagtgatcg tctctactat attgacgatg cttggcggtc atcttcctgc acgaatggca
    37501 gcgaaaaaag acgccgctat cgctttgcgt gcagaataag gttgtgacaa ccacatttag
    37561 atttgagtca taagagcgct gaaatagtag aaaacagcaa gaaacgagga agtaatatgt
    37621 tccttgtttc gcgctgtttt ttttaattat tttttataat tctggtatac ttttctttcc
    37681 tttgtaccac aactcttctg gcgtatacct gtcttttcca tttatgaata ctgttccatc
    37741 tgcttcaatc gttacttcag tcccaatcca catattacga gaaagtgcga gaagattttt
    37801 tctttttcct cttccttttt ttccaaattg caaggcatcg gttatctctt ctgtccaatg
    37861 agatttcacg tattttactt ctccgttttc aaatgtaact ttcaaaatat tcgctatatt
    37921 ttccactttt actgctttag gaaatgactc ctccattttt ttcactttcc ttcctccaac
    37981 actaagtatg cgctttcttt tagtgataag tatatcatag tttattctat tatcaaaaaa
    38041 acgcatccat tccattgtag aatactttac cgaacaagtt gaattgtttt cctctttttc
    38101 accttttact atagataggt aaagacaact aaggaggaat ctctcatgac agatcaaccg
    38161 tatgatttaa gaaaagtcat cgaagaacta aaaacacttc caggtcaata tcacgaaaca
    38221 gatattgaaa tcgatcctga agcagaatta tctggtgttt accgttatat cggtgctggc
    38281 ggtacagtaa aacgtccgac tcaagaagga ccaactatga ctttcaacaa tatcaaaggt
    38341 ttccccggca cacgtgtgaa catcggaaca atggctagtc gtaaacgtgt aggtgaaatt
    38401 ttgcaccatg attatcgaac acttggtcgt ttcttgaaag acgctgtaga aaatccaatc
    38461 aaacctgtga aagtagacaa agcagacgct ccagcacaag aagtcgttca tctagctacc
    38521 gatgcagact tcgatattcg caacattttg cctgcaccta caaatactcc tgaagatgct
    38581 ggtccataca tcacaatggg tgtagtctta ggatcagatc cagaaaaaac aatgagcgat
    38641 gtaacgatcc atcgtatggt tctagaagat aaagatacga tcggtatgta catcatgcct
    38701 ggcggacgcc acattggtca cttccaaaaa caattcgaag ccttgaataa accaatgcct
    38761 atcactatca atattggttt agatccagca attgccattg gcacgacttt cgaaccacct
    38821 accactccac ttggctacaa tgaactatgg tgtgcaggag ctttgcgtaa cgaacctatt
    38881 caaatcgttg atggtatagc agtcgatgaa gtgggtattg ctcgtgctga attcatcatt
    38941 gaagcagaaa tcatgccaaa tgaaacaatg caagaagata tcaacacgaa ctcaggaaaa
    39001 gcaatgccag aatttcctgg ctatactggg gatgctaatc cagctgtaca agtagtgaaa
    39061 gtcaaagcag ttactcaccg aaaagacaat ccaatcatgc aaacaacgat tggtcccagt
    39121 gaagaacacg tatctatggc cggtattcct acagaagcaa gtattcttgc cttggtcgat
    39181 aaagctattc caggaaaagt agtaaatgtc tacaatcctc ctgcaggtgg tggtaaattg
    39241 atgaccatca tgcaaatccg taaagaaaac gaagcggatg aaggtattca gcgtcaagca
    39301 gctattcttg ctttatcagc cttcaaagaa ttgaagacag tcattttagt cgatgaagat
    39361 gttgatattt tcgacatgaa cgatgtagtt tggactttca acactcgttt ccaaggagat
    39421 aaagatatca tcgtgatccc aggaatgagg aaccatccat tggacccatc agaattacca
    39481 gaatataatc ctggctctat tcgtttccgt gggataagtg caaaaactat ctttgacggt
    39541 acagttcctt tcgatatgaa agaacatttt gtacgtgcaa aattccaaga agtagaagat
    39601 tgggaaaaat atttgaaata atgaatgatc tgcaactgtt ttgacatcat aaaaaggagt
    39661 gcggaaaatg gaacaattgg cacaacggaa gaaaaaaatc gtgattggtg taagtggcgc
    39721 ttcaggcacc atttatgcca tcaacctagt gaagaaacta aaagaatatc cgatgataga
    39781 aacacacgga atcatcagtc catgggcaaa acagaactta aagctggaat ccgacttatc
    39841 actaactgaa ttcgaaagcc tgtttgatta ccattattca aacaaagatt taggtgctgc
    39901 tgtcgccagc ggatcatttt tgaccgatgg aatgatcatc gtaccggcaa gtatgaaaac
    39961 agtcgcaggt atctctgttg gtttaggcga caacctgatt gcacgtgctg ctgatgtaac
    40021 aatgaaggag caacgaaagt tagtcattgt tcctcgtgaa acgccattaa atgccattca
    40081 cttggaaaac ttgactaaat tagcacgact aggtgtgcaa atcataccac caattcctgc
    40141 tttttataat cagcctcaaa cgatccaaga tcttgttaat caccatacaa tgaaactttt
    40201 ggatcaattt gaaattgtaa atgagcattc aaaacgttgg cagggtgatt aatatggaaa
    40261 aagaaacgat agttgaagta accaaactag gtttcacgat ccaagctgct gttaaagtaa
    40321 tcggtaaaga cttattgatc actttaacag gcggagatac accacatatc ggaacagtca
    40381 ctacattttc tcgtgataca gagatgcaaa ctgtacgttt ccctagccac gatggtcggt
    40441 tccataaaga cgatgtctta gccgaaaaaa tagctgtgat catcaaaact gccgttccgg
    40501 gaagttgtac gatcacttcc ggtatccatg ttgaccatat ctccaatgca caaatcaacg
    40561 cctcttttcc gatgtcagaa gagttaggtt acaagatttt agattggttg gaaaaaatag
    40621 atttccatat caaagaacca gtttattata aagatggtga aaaaccatta tgaaagaaga
    40681 ggttgtgaaa tcagcgttat gacctgagta tgagagaaaa aaatggaaaa atagcttctc
    40741 atattttgcc gtttttgagc taataccgag ggacagctga ttgaacgccg tttatcaaga
    40801 ggttgtgata aagccgcttc gtttcgagta gtaagaaaaa ttttgtaaac atgggtttcc
    40861 acatttttgc ataaatttga cttactaccg agaaactggc ttttgaacac cgtttatcaa
    40921 gaggttgtga cacgtctttt gtcacaacct cttttatttt tgcttcactg taactgctac
    40981 aatatcaccg atttcttttc cgatgttttt acgaatatct tttcgtagtc caaggatgta
    41041 gcagactgaa ccatctggat ttttcacgcc catattcacg atactgcctt catatggcac
    41101 attgtcaaaa gttgcttgga ctttaactct tccagcaccg aattcttttc ttatatcata
    41161 cggaaaaata atatatgccc cgcccttgcc gacttcggaa gcatagattt ttgattcaaa
    41221 agaataaatt tccctcattt tatttctcct attttttaca tctagtatag catagcaaaa
    41281 aaaagaagga actgtgacaa aagtcttgtc gcagttcctt ttttcgaata aacggtgttc
    41341 tcctgccaac tcctcggtca caagtcacaa tagtcaacaa acatgagaag ctgttttttg
    41401 aaattgctcc ttgtgactca gagttaaacg gcaggctcac aacctcttcc ttaaacggtg
    41461 ttcaaaagct tgcatctacg gcactaagcc caatcagatg aaaaatatgg agtgctattt
    41521 ttatctgatt gttcttactg cttgatgcag agcagctttt tcacaacctc ctacatggtg
    41581 ttcaaaatat tattcgcctt ttgcttcttt cactaatttt gcgaatgctt gttgttggaa
    41641 ttcagcagct aaggctttcg tacgttgttc ttgctctgct ttgctcattt ttggattttc
    41701 agtcattgct tttaccatac cgtcttgaac ataagctttt gcttcacttt ccatcgtact
    41761 ttgctgtttc ttcactaatt cagctaactt ggctgcttgg tccgtactta attctaccca
    41821 tgtctctggg attttgaatg tattcactcg gctgtcatat tcgtgattgt tatcagcaat
    41881 attgaaccag aatttataca tgtcgttttt gtattcccaa taagtagttt tcgtcacttt
    41941 ttcagcatcg ccgtctgtct tttctacttt atttgtttcg ttatccgtac cagttgcttt
    42001 tggtttttct tgtttttcat ttgttcggta aagatacact ttttcttttc cattccctaa
    42061 agctttatac aaaagcatat ctgctccttg ggaatcagca ctggaaacaa ttttttctgt
    42121 ttttgtttcc gttacttttt ccattccaaa gtgattggac aaattagcga cgatcaaact
    42181 tagtgaagcg acgaagatta gtccgaaaat caaagataaa aaggtttgcc atgttttttt
    42241 agcgaacacg ttcgttaaag caaaagcaag caagctgaca atcaaaactc caataatcat
    42301 gcattatccc ccgctttcat tagaacaggt tcttttttct tcaataaaaa ggcaataata
    42361 aagccaagaa tcccaaagat gatcgctacg ataaacgcgg catggtaacc tgacagtgtg
    42421 gcattgattg cctgatcttt ataagctaat ggcgtattat ccaatacaga tttgcttggg
    42481 atatttgctt ttgtcacatt cgtcaacaca ctgatcaaaa tagctgtacc aatcgagctt
    42541 gcgatttgac ggaatgtatt atttaccgct gtcccatgac tgatcaagtt atttggcaat
    42601 gcattcatac cagaagtcgt taccggcatc atgaccatgg agatcccaaa catacggatc
    42661 gcataaagga tcacgatata taaaactgga gtcgtttttg tcaagaaagc aaatggtgca
    42721 gttgccgctg tcaaaatgaa catccctgtg atggctaaac gtttcgcgcc gtatttatcg
    42781 aaaatcgcac ccgtcacagg catcatagcc cccatgatca atgcacccgg caacaacatc
    42841 aagccagaat ggaatgctga ttctccacga atattttgaa tatacaatgg tacgaccatt
    42901 tctgccccta ccatcgccat atttgttaca ccagctaaaa cagctgcaat cgtgaatgta
    42961 gatgatttga atacacgaag ttccaagaat ggatgttcca aatgtaattg acgccatgag
    43021 aacaaggcga tgaaaatcac accaacaact aagaaaccaa ttactttcaa gcttccccag
    43081 ccgtcatctc ctacactaga aaatccgtaa agtaaactac cgaatccaat cgttgataaa
    43141 atagcggata aaatatcaat gctaggattt gacaatggaa taacactttt cattaggaag
    43201 aatgctaaaa ccaagacaag aacaacgatt ggcagtacca taccaaacaa gtcacgccaa
    43261 tggtagttat caatgatata accagataat gtagggccta aagcaggagc aagtccaata
    43321 acgatccctg acatccccat tgctgatcct cttttttctg gcgggaaaat agatagcatg
    43381 atattttgca ataatggcat cgatactccc acaccgcaag cttggatcaa acgaccgatt
    43441 agtaatgtac taaaattagg tgctgtaaag caagtaatcg ttccgatcaa aaagatcgtc
    43501 atggcagaaa gatataaatt tcttgaactg aatttattga tcagccatgc tgttattggg
    43561 atcatgatcc cgttgactag caagaaacca gtcgttaacc attgcacatc agacgctgaa
    43621 atatcgaaag ctttcatcaa tgtaggaaaa gctgtcgtca ataaggtctg attcaaaacg
    43681 gtacaaaatg taccaattaa taagacaaca actaaaagtg tccgattata tggttttccg
    43741 taaatatcta cgggttggtt gttactcata tttctccttc tttctgaatt ttttctgaaa
    43801 ctactagaat actttagtcc taaattcgct atttgtcaac ctacttgaca cataagttaa
    43861 tacggatata attaagggaa gcaagaggac aaaaggagtg acaaaaatgg ttggggcttc
    43921 ccttaaacgt ttgttggaaa atgatcaatt ggttgttggc atcagtgaat taagtgatat
    43981 cgtaggtgtg tctccacgtc aattaaggta ttgggaacaa aagggattca ttcgttcgat
    44041 cgaagctgat gcgaattgtc ctagaaaata tcgtttacct acagtcataa aagtagaact
    44101 gatcaaaaaa tatttagatg acggatttac tttgtcaaaa gctgcagaaa aggcagaaat
    44161 ccgccaaaag aaaatgcacc atgtccgtaa agttttttca aaagcaatca aagatatcga
    44221 attgatcgac gaacgctata cgattttaca aatcggtaac ttcaaagaag agaatgctaa
    44281 gatgtacatc attcatgaca acgagacaga cgaattaact tacaagctcc ttcctgtcga
    44341 tgaaaaagct gactacgaac agttgttaaa agaactctaa acgccactaa tctcctaagt
    44401 accaagcttt ttaataagta gatgctacat aaatacttga aaaaaggcct gtttgaacat
    44461 aagaatatcc gaacggtgat ctgcgtttct ttcatataga aaggaattct ccatcactgt
    44521 tcggatattt ttttattttt ttcggttact ttcttctaat acatctgtta gccaatttgc
    44581 atattctgtt tcccgattga tcgcatgcgc taaaatcagg tagtgtccgt aattttcatg
    44641 tttttctttt tcagtaggaa agatcgtttt ttttcgctct aatagatgaa gtcgtttttc
    44701 ttcatgaagg tcacgctgct gctgaataat ttctactaat gccggatcat tcacgtcttt
    44761 gacgaaatac aatttcagaa caaattcgtc tcgattaacc ggtagttcat tggtgggtgt
    44821 atgaatccac tctgccaatt gttccttccc ttcttctgtt aattcataaa ctttcttttc
    44881 caatttttca cctgtgatct ctacttgatg ctgaatgatt ccttgctctt ccatttttgc
    44941 aagttcagga taaatctgac tatgttttgc ttgccaaaat tctcctattt catttttgaa
    45001 cgattgattc aattcatatc ctgtcatctt cttcttactt aataacccta acaaaatata
    45061 tcgaagtgta ttttttcttg ccatcagttt tccctccttg acaaatcttt aaatccattc
    45121 tatcataaat gagtaatcga aaacatgtag tttattacat gttttaattt ataaggagat
    45181 gcttaaacat gaaagaattt aaagaattgg aagattttat tggaactcac tttatctata
    45241 cgtatgacaa tggttgggaa tatgaatggt atgccaaaaa tgaccatact gttgattacc
    45301 gtatccatgg cggaatggtc aaaggccgtt gggtaaaaga ccaagaagct gatatcgtta
    45361 aattgacgga cggcgtattc aaaatcactt ggacagagcc aacagggaca gacgtggcat
    45421 tggactttat gccaaatgaa aagaaacttc acggaatgat ctttttccct aaatgggttg
    45481 aagaacatcc agaaatcact gttactttcc aaaacgaaca tatcgcagaa atggaagcag
    45541 ctcgtgaaaa atatgaaact tatccaaaat tacttgttcc tgaatttgct acgatcactt
    45601 atatgggtga tgcaggtata gacaatgaag atgttattag cgaagaacca tacgatggaa
    45661 tgccagatga tatccgtagc ggaaaatact tcgatgaaaa ttataaacga gtaaaataag
    45721 gttgtgaaag agaggttgtg aaatcagcgt tctgacctga gtattagaga aagaaacgga
    45781 aaaatagttc ctcatgtttt gccgtttttt gagctaatac cgagggtcag ctgattgaac
    45841 accgtttatc aagaggttgt gaaatcagcg ttatgctttg agtaataaga acgctagtta
    45901 gaaaatagct tttcatattt tctaggctag tgggcttatt accgagaagc agccgtttga
    45961 acaccgttta tcaagaggtt gtgaaatcag cgttttgacc tgagtattag agaaacaaat
    46021 ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt cagctgattg
    46081 aacaccgttt atcaaagatt gtaacaactg catattcaca cagttaaatt agtgacttca
    46141 aaatgaaata atgctcccag caaaaaatta ggctgggagc attatttcgt tattctgttc
    46201 atcaaaaatc agcgttttga ttccatttca tcacaagttc ctcctcattt actgcttcat
    46261 ctttttgctg tgtttgaatt gtaaaacctt gtgaaagata gaaatgaata gctgattgat
    46321 ttttttgata gactgtcaaa gtcagtgttt tattggtttc ttttattttt ttcattagtt
    46381 gcgtaccgat tctttgttgc cggtattccc ttttcacaaa gattcctgcg ataaactgat
    46441 caatcacccc agcaaaacca ataatttctt gatctatttc tgctaagtaa atcactgcat
    46501 ctggtaaagc tttcctaaca gattctactt gattctccca ataagttgat ggaatgaatt
    46561 catgagaatc caagttagca tgcagccaaa tcgatagaac agattccagt tctttggcag
    46621 acaattttgt tgttgtgcgg atcatttttt ctctccttac ttattcattg attttaaaat
    46681 aaaaaccatt tttttgaagc tcttttacaa gttttcgccc ctcaaaaaag tatatcttct
    46741 cttgttttac ttttctaata ccaaaagaaa cagcaacttt aggatcatca gctgaaacga
    46801 ttcgataagt tccaccaggc aattgatcca gcttgacttt cccatgatgg tcgctttgta
    46861 ctgaaaacgg cacaccattt ctagtaagtg tttttttctt atgtttcaaa atgaatgaac
    46921 taccatttac cggtttccct gacgaatcac taataataaa tgaaaaatca agcacttttt
    46981 tacttaatct atactgatag attagtcgtc cgataaacac taacagtatg actacagcta
    47041 ttatcgttcc tgcaagaata aaccattgcc gcatggttct ttcctgattt ccttttcctt
    47101 cttctttttt taccgattcg ttataaggaa tccgatgtcc tgtcactagc aaacgatgcg
    47161 tgttgatcat atacggcgta catgtaagca aggtaactaa gtcttctcct tctgaaatcg
    47221 ttagaacaga cgtgtcatct ggcagtacca cttgtatatt tttaacttga taagccaatt
    47281 tctcccccaa aacagtcaaa ataaacgtgt ctccgttttc taaacgatct aattcggtaa
    47341 ataaccgtcg attaggtaaa ccgctgtgag cagagatcac tgaatgcgtc gaatttccac
    47401 caagcgggaa agaggttcct tgcaagacgc ctgctccatt ttcaaggatc ctaccattga
    47461 gccgatcata aagtgaaata tttaattgga tcttaggtat attgatactt ccgatcagcc
    47521 attcctctaa ttggctggat gttgtacttt catttctgtt cttttcattg aaaggatcag
    47581 taccaggatt gaatccttgg tcagttaatc gttggttttt ctcctccatt ttctttcgct
    47641 gcacttcaat ttcagctgcg gtccgttttt gtacttgttc gatacgtttt tgatcaataa
    47701 aactatttaa agaatccaca taaaatggat ataaagcgat cagtaaccct acgacgatac
    47761 atattaataa aaacaaacga aacaaaagtc gacgagactg tttttgctta ctctgcttca
    47821 catgtgtcct cccttgcaag tttttttgtt ctttctttta ttatatgggt tcaggaaaaa
    47881 aatagaaaat gaaagccgat gaggttgtga aaaagctgtc ctgcatcaag taataagtac
    47941 agcccaaaaa aatagctact catatttttt gttgggttgg gcttattacc gcagatgcaa
    48001 gcttttgaac accgtttaag gaagaggttg tgaaaactgc gttatgcttt gagtaataag
    48061 agcgctagtc agaaaatagc ttctcatatt ttctaggcta gtgagcttat taccgagaag
    48121 cagctgattg aacaccgttt accgagaggt catgaaaaag ctactttgtt tcgagtatta
    48181 agaaaaattc tgtttcaaaa caaggagatg caacaaaact tatgtcgcat cctctctcct
    48241 tagaactttc acttatgttt ttcttgtaaa attcataaat attttcatga attttctaat
    48301 ttcttgccta cctctcgcat aggatatatg atgaatttat ctaaagtttg caaaggagtt
    48361 cgacaattga aaaaatatgg cccattttta gcaatttttt tcattgcttt ggcatttctc
    48421 atgcctcatt tattcacaca taaactaatt ttgggtgctg atgccccatt ccattacaat
    48481 cggttctatg atgccgcgca acaaattaaa tatggggatt tccattattt tccttccctt
    48541 tatggattcc agcaaagtgg caggatcgtg aatgccgttt acggcccatt tttcgcttat
    48601 ttacaaggat tgatcgtgtt gcttgcaggt agctggtttc attatcagat tctttcaaat
    48661 ttcattcttt atcttatttc aggattttct atgctgattt tactgagata tgtcaatgtt
    48721 cgaaattggg taagtgtacc agtagccgtt ttattcatga gtacgtattc tattcaatac
    48781 tggtctatca accaaggatt cactagctgg gggacttgtt tttatccttt atgtatgatc
    48841 cctattgctg atttcgtcct tcataaaaag ttccctgtta tcaaagtcgc tgtctcagtg
    48901 ggacttatga cacagatcca tttactgtct tcgatcatgt tagctttgat gtatatccct
    48961 ttttatcttt attacttttt taaccagact gaaaaaggaa aagcactgct tcaactgatt
    49021 aagtcggtct tgctttattt tgttctgaca gcaaatattt gggcaggatt agctattgtt
    49081 tacagtggga ataagattct tcctcccttt gttaacagag aaatgagtca aaaggcgata
    49141 gatctggatg gcagttattg gctccattac cctaaggcct ttcctatatt gatattatca
    49201 ggttttttgc tttttatttt ctttcaacaa aagaacacct tattccaaaa aatcgtgttg
    49261 tacacaagtc ttgttttctt ttttctttct actagcttga ttccatggac gacattgatc
    49321 gaaaaaaatg tgccttttat tagtttgatc cagtttccat ttcgattttt cgtgccattt
    49381 acgctattgc ttctacttta ccttgccctt tcactgaacc agtggtctga acaaaagtgg
    49441 ttccgtcttt taagtatatt tggcattctt attttttcag cacagacgat ccagaactta
    49501 taccagcatc tggaaaagtg ggaaaatgaa acatttgtca gtcgacatac ttatctattc
    49561 gatacgccgg aagaagcaag gaaaacattc ttcagcaaag atatgtctac cagcttgttc
    49621 gttttccaaa aaaccacacc tgactatctg ccaatctata aggagacaaa agacaataaa
    49681 tacgaccggt ataacgaaga aatactagaa aacgaaacaa cttttataaa aacacatcca
    49741 ggcggtcttt tgacaatcaa gtggaaaaac actggggaca aaactgttca tatccctgtt
    49801 atcgtctacg atcggactgt gctacagcaa aatggaaaaa cattgactga ttatgaagta
    49861 acagatatcg ggaccccaat cgtgaagcag caaaaaggaa tcaacgagct cacgcttcat
    49921 tatcaaacgc caatctattt ctatttcatt ctttctctta ctttgattgg ctggtttacc
    49981 ttgctatgtt tatttattta tcatagatac aagcttttac gtgcttaggc aaaagggata
    50041 aatacatccg atcaaaaaac tctccaaacg gtataatcgt ttggagagtt ttattttaat
    50101 cagttttttt atgttatatg ttatattttt tattcgttca catctaattc gatttccacc
    50161 ccgtagtgat cactgatgat tggactatcg atcccatcaa aaatccgttg atatttttta
    50221 acctgcattc cttcagggac gaaaataaaa tctatacgca atttttctgt attttgttcc
    50281 caaccatcta ttttcttttc cacagtagct gcttttcctt tttcattcgc gacaacatac
    50341 gaatcttgaa gaagaaggca actttctctt acagtataat aaccttgaga gtctactgga
    50401 ttattgaaat cacctaggaa aaaaagtggt acgcgagttt ccaaaaagta gttttccaag
    50461 tttttccact caaaataaaa gccacttttt ttatctgtcc accaagaaaa gtggcagctc
    50521 gagacaaaaa tgtcctgatc atctatcttt gttttcccaa tcaagatctt gcgtgtctga
    50581 taatcttctt tcttttgaaa ttgggagacg gtaaggacct gactttccaa tgtttttttc
    50641 gtaagcagtg catttccttc ttcatattta tcataaccga tgtggctcat ttcccaactc
    50701 caaaaatact cttttccgtg ttcctttaaa agctgaacta aacggtaggc aaaattatct
    50761 ttacggatag gtacttgatt ctttaccgga cagaacttca ttagcttcgc aggttctact
    50821 tcttcagatt ccagtaattg gttgatttct tgtaaagcaa tcacttcata atcttctaac
    50881 aggatcttat ctgctaattc ttgcaatttt ttctctggtt ctggctcaag ccagctatgc
    50941 gtgttgagtg tcatgaattt cattggtttt ctccttgtct tcatttcgaa acaaaagggt
    51001 tgtgacaaaa tttttgtcac aacctctcca gcaaatagtg ttctcctgct aactcctcag
    51061 taataagctc aatggaccag gagctatttt ttgtttggct gtttttatca cttggtgcag
    51121 ggcagctttt tcacgacctc tccactaaac agtattcaaa ctgctgcttc tcggtaataa
    51181 gcccaaccaa acgaaaaata ggaggagcta tttttgtttg gctgttctta tgacttgatg
    51241 caggacagct ttttcacaac ctctccttac attttaatgg agccgatcac tttaccagct
    51301 gtcactgtac ctaattgctt gatcactact tcttctactt tatcgtttgt aaatacgatc
    51361 atgatcgttg gatctttttc ttcgttttgg atcacatcaa gatccatctc agctaatttc
    51421 gttcctgttc ctacttcttg gccttctttg acagcaatct caaaagctgg ctgattcatt
    51481 tggactgtat cgattcccat atggatcaaa acttcgatac catctttcgt ttccagccca
    51541 attgcatgtt tgctagggaa aatacttgtc acttttccag cgatcggcgc aacaacttct
    51601 cttgatgttg gaataacagc aaagccgtca cccatcattt tttgtgagaa taccggatct
    51661 tttacttcag ataaaggaat cacttctcca gaagctgcgg aaactagttc ttgttctata
    51721 cctaagtatt gcttactacc tgcttgtcca tttcctgtag aaaccatttc tggctctgga
    51781 atctctgctc ctgattgcag cagatcttcg atatctgatt tcaagacatc tgcttttggt
    51841 ccataaactg cttggactcc attgtctttg acgatcaatc ccatcgcccc tgctcgtttc
    51901 caggcttctt cagtccctac tttttctcga tcatttacac ttacgcgtaa ccgagtcata
    51961 caagcatcga catctttgat attttctttt cctcccagca aatgaatgat ctggaccact
    52021 tgctgatctg ctgttcctgt tccgctgtta gatacaacgg tttcttcact gtcattgtcg
    52081 taatttccgt tacgtcccgg tgtagcaaag ttgaattttt tgatcaagaa atttgctaag
    52141 aaatacgtaa caacaccaaa agcgatcaca cacaacacga agttgataag atctccacct
    52201 agacccgctt tgatagctaa cggtgtccga gtcagcaatt cgatattccc aaatgaatgg
    52261 acccgcaatg gcaagatatc tgccatcgca aatgcagccc cttggatcac tgaataaatg
    52321 acatataatg gcacagcagc aaacataaac atgaactcca atggttctgt tacacctgtt
    52381 agaaagacag ccaacgctgc tgaaaaatac attgatttat atcttgcttt cttatcagga
    52441 tcgacattac gatacatcgc aaaagccatt cccatcaaga tacctgaaga accaatcatt
    52501 tgtcctactt tgaaacgagc cggcgtccag ttatgcaaca caaactcata ttgcgacata
    52561 tcccctgcac ctttaagatt cactaaatca gtggcccaag ccaaccataa cggatcttgc
    52621 ccaaatactt gtgtccccgc ctgtgcgcct gataaaatct catacgatcc gccaagctgt
    52681 gtatagttga taggaatcgt cagcatatga tgcaatccaa atggcaatag taaacgttct
    52741 aatgttccaa ataaaaacgg tgctagaatc ggtgcgcttt cttgtgattc tgcaatccat
    52801 aaaccaaaat tattgattcc agcctgtacg tttggccaca ccacagcaag aatcaatgcc
    52861 acgattgttg accataagat aacaacaaac ggaacaaagc gctttccatt gaaaaatgac
    52921 aaagcatccg gcaattttct ataattatag tacttattat aagccattgc cccgacaaat
    52981 ccagcgatga tccctacgaa tactcccata ttcaaagctg gtgcttccaa tacacttgta
    53041 aaaaatcctt taaccatgat cttcgtaccg aacagagtgt gggtaaatgc atcttcattc
    53101 gcaagcatgt cacttgtgac accaaagata gccccagtga tccgattgat taaaataaat
    53161 gagatcccag ctgcaaatgc tccgcctgct ctttcttttg cccaacttcc tcctattgct
    53221 aaagcaaata aaatatgaag attaccaata attgcccaac caatattttc aattactccg
    53281 cccgttgtga caagcggcgt ccaatcagga ttgatcaagg gaattgattt ccctatactg
    53341 atcatcaacc cagctgccgg cataactgct accacaacca ttaaagcctt tccaaatttt
    53401 tgccaaaact caaaactaaa tatctttttc attccgttta gctcctttac gcaatcggtt
    53461 tcgttaaacg tattatagcg tgcgttttct tttttgtaaa tgctttattt tttatttttc
    53521 tgtctatacc attctataaa acctataata cgtgtgttcc cttctataat tttctcctaa
    53581 aaatttattt ttttaagctt ttttctatga aatattttct tttgtctatt gacttgtgca
    53641 atcggttgcg tttattatga acgtataaaa cagctcatta ggaggagaaa aatgaaacag
    53701 atcaaacgac tatttgctat tgatccatgg aaaatcagca cgacacaatt agataaaaaa
    53761 aatcttcgac tgcaagaatc tttaacaagt attggaaacg gttatatggg gatgcgcggg
    53821 aactttgaag aaacttactc aggggaccac catcaaggta cttatttggc gggtgtctgg
    53881 tatcccgata agacacgtgt cggctggtgg aaaaatggtt atccagaata ctttggaaaa
    53941 gtgatcaacg ccttgaactt tatcgcgata gatatttcga tcaacgatca gctgatcgac
    54001 ttggcttctt tggaaccaga agacttttac tgggaattag atatgaaaaa tggtgtatta
    54061 tctcgtacat tcacaatcac tacttctact aataaagtac gcatttcctt cgaacgtttt
    54121 ctgagcattg tcaaaaaaga atcagcttat atctatttaa ctatagaaat gcttgaaggt
    54181 agcggaacag tcaaactcgt atcaaaattg gatggaaatg ttcaaaacga agacagcaac
    54241 tatgatgaac atttttggaa tgagattgat cgtggaacta tagaagaaat cggctatttg
    54301 acgactaaaa ctattccaaa caactttggg attgaacaat ttactgttac tgcagcaatg
    54361 cgccatttcg ttaatggaaa atcagtcatc cctgactatc acgaagatac tttagcatta
    54421 catggttgct tgtctgcaga attatctcaa ggtgagacat ggtcagtgga aaaagaaatt
    54481 atcgttctta ctagtagaga tattccagaa gcgcagcaag cagaaaaagc tgtctcagaa
    54541 ttaacgaacc agacaaacaa ttatatccaa gcaaaagaag agcaaacagc ggcttgggca
    54601 gaacgatgga agcttgccga tgtggtcatc gaaggagacg aagaagcaca acaaggtatt
    54661 cgattcaatt tgttccaact attctctact tactacggcg aagatgaacg attgaatatc
    54721 ggccccaaag gattcacagg agaaaaatac ggcggagcca cttactggga tacagaagcg
    54781 tatgctgtcc ctctttatct tgctttagcc aaacctgaag taactaagaa tctgctgaaa
    54841 tatcggcata atcaactgcc gcaagcaatc cacaacgctc agcaacaagg attaaaaggc
    54901 gcgctttatc ctatggtcac cttcactggt gtcgaatgcc ataacgagtg ggaaatcaca
    54961 ttcgaggaga ttcaccgaaa tggcgcgatt gcctatgcaa tctacaatta tacgaattac
    55021 accggcgata cggaatattt gaaaaatgaa ggactggaag ttttagcaga aatcgcacgc
    55081 ttctgggcag accgtgtcca tttttcaaaa cgagcaaata aatacatgat ccacggtgtg
    55141 accggcccaa acgaatatga gaacaatatc aataacaatt ggtatacaaa tacgatcgca
    55201 gcttgggtct tgcgttacac gcgagaaaat tatttaactt atcaaaaaga agctgctatc
    55261 actatcagcg aagaagaact agccaaatgg aaagatatcg cagagaacat gtacttccca
    55321 gttgacgaag aattaggaat ctttgtccag catgacacat tcttagataa agatctcatg
    55381 cctgtatccg atattcccac aagcgatctt cctctgaatc agcattggtc ttgggacaaa
    55441 atccttagat cttgcttcat caagcaggca gatgtactac aggggattta tttcttcaat
    55501 gatactttta caaaagaaga aaagcaaaaa aactttgatt tctatgaacc aatgactgtc
    55561 catgagtctt ccctttcgcc aagtatccat tcgattttag cagcagaatt aggaatggaa
    55621 agcaaagctg tagaaatgta ccagcgaaca gctcgcttgg atttggataa ttacaataac
    55681 gacacagaag acggcttgca catcacttcc atgactggta gctggttgtc gattgtccaa
    55741 ggatttgctc aaatgaaaac tgcccatgat cagttgagat tcgcaccatt tctgccaaaa
    55801 acttggaaag cttattcttt ccatatcaat tatcgcggac gtttattgtt catctcagtc
    55861 acagatgaac aagttacttt cgagttatta gaaggttctc ctctagaaat ttacgtctat
    55921 gaacagtctt attctttagc ggatcgtcta gaaataacgc taagaaagga ggaagcaaat
    55981 gtttaaaggg gtattgtttg atttagacgg tgtcattacc gatactgctg aattccatta
    56041 tcgtgcttgg cgaaagcttg gtcaggaaat cggcattacg atcgaccaca cattcaacga
    56101 acagctaaaa ggcgtcagcc gagaagactc actctctttg cttttagctt atggcggaaa
    56161 agaacactct ttttcaaagg aagaatttgc agaattagcg aaaagaaaaa acgattatta
    56221 tttagagatg atccagacga tcgaaccaaa agatgtcttc cccggggtcg tgcctttact
    56281 ggattcttta aaagaagaaa aaatcaagat tgctcttgct tctgcaagta aaaacgggcc
    56341 attcctactt gaaaaaatgg gtcttacacc ttactttgat gctattgccg acccggcaga
    56401 agcaacaaac ggaaaacctg cgccagatat cttcctcctt gcagcaaaag ctgtcggact
    56461 aaaagcagaa gaatgtatcg ggatcgaaga cgcgcaagcc ggtatccaag ccatcctctc
    56521 aagtggcgct caaccaattg gcgtgggacg tgctgaagac cttggagagg agatcccgct
    56581 tgtccctgat acgacttttc ttacgatcga ttacttgaaa gaaaagtggc atgaccatgg
    56641 ataatccaga gatctccgtc gtaccagttc cagagactga aataaagaaa atcacattga
    56701 agaacgacta cttagaagtc gttcttctca attacggtgc ccgcctgcac caaatcttcg
    56761 caccagacaa agaaggaaaa tcagaaaatg ttttattgtc ttacgatcgt ttcgaagatg
    56821 ttctaacaga caaaagcttt tttggagcaa ctgtcggacc tgtagccgga aggatccggg
    56881 atgcttcttg gggtgagcat agattggaga aaaactgcgg atcacaccat atccacggtg
    56941 gcacgaaagg ctggtctttc caattttggg aagtagaagt tttcaaaact ccatcatcaa
    57001 taggtgtggt cttttactta aaagatgaat tttctaccta tccaggacca atcactgcta
    57061 cgatcactta ccgtttaaca aaaaacgaat tagagatgac aacctctgct agtagccttg
    57121 ttgaaaccat ctgcaatccg accaaccatg cctacttcaa cctttcagga aatggaacaa
    57181 gagacatcta tgaacatcag ttaacggtct ttttagatgg tgtgcttgaa ttagatcaag
    57241 aaaaactgcc tactggaaat tggacaaaaa aagaaaaact gccaatcgac tttagaaaat
    57301 ccccaacact gcaagagatc cttgcatgtt atccagatgg actagatgat gtatttcttt
    57361 tgcatcatcc taggctttcc aaaacttcct tacaattatt cgagaagcat tctggtcgcc
    57421 aaatgactat agcaacttct aataaaagta tggtattatt ttctacgact ggattcgaag
    57481 ctgatttctc tgtcaatgga aaacagatgc attcaaacta cggattagca atcgaaccgc
    57541 aagaaatccc tgatatcgtt catttcccca aatttggatc gatcaacctc catcctggtc
    57601 aagaacggat cagtcagacg atttatcggt tctctgcaca ataacaagag aaaggtgttt
    57661 ttatcatggc tattaccgta aaagatgtgg cgaaaaaagc cggtgttgcc acctcgaccg
    57721 tctctcgtgt catcaacgat catcctagta tttccgagac aacaaagaag aaagtccgaa
    57781 aaattatgga tgaactaggt tatgttccta acatgactgc ccgcaatcta ggaaaacgca
    57841 tttccagtgc catcggcgta atcttacccc ctcttgattc aaaagagcgg atcggtaacc
    57901 cattttatct tgagatcatg gaagcaatca acgaagaagc gcggaattat gatatgacta
    57961 cagcaatcgc tactgctaaa aattttgaat tgctgttaga gaacgtccag cgtatgcatt
    58021 tacaaaaaca agtagatggt tttattctag tgtattcaga tagtcaagat actgtcattg
    58081 attatttata ccaaaaccac atccctttca ctttgatcgg acagccttat catcatgaga
    58141 acgaaatcgt ttatgtagat aatgataacc agcttctagg taaacaagca acagaatttt
    58201 tgatccagaa tggacataag aatatcctgt ttgtgacaaa cacgactcat gaaagtttgt
    58261 atttcgaacg atatttcggt tatcaaaaag caatgatgat ggctggtctt cctgcgttat
    58321 ccagtgtagt actagagcat ccggaggatt atctgtcctt tgagaaaacg ttgaaagaaa
    58381 acgaagccac agcattggtg gtcattgacg atatcttcgc ccttcgcacg atgcagttgg
    58441 cgcaaatgta tggctatgtc attccagaga cactctccgt cattagcttc aacaactcga
    58501 tcttctcaac attaacacac ccttacctta caagtatcga tatcgatatc tccgaattag
    58561 gacgtgtcgc tatgcaaaag ctgcgcgaat tgatccatga agaattgagc agtggcgtac
    58621 gtttggtcat ccctcatcgc ttgatcaaaa gagagacagt cgttccttta gaaaacccat
    58681 actaaatagc tgactcacta aatacgctga ccatttatcc tatggtcagc tttttatgta
    58741 aaagaagata ctacttctaa cacattgttt atctgtaaaa aagagcgtgg gacaaaagta
    58801 aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaagcaga agcaactcct
    58861 tcggaaataa gctgaaattc acaaaaattt aaaaagcaat tttcttgagt ttcttcttat
    58921 ttcttggagt taaacgcttc tgtcccagcc tcttttttcc ttccttcaat tttttacacg
    58981 tcgacgattc aataaaaaac cgcctaatat tgtaaagaca ggtgcaagaa tcgagaaaaa
    59041 ggcgaatggc aagtaatgca acgcgctaac tttcatcgtt cccataatga atgtgccgct
    59101 gactccccac ggtacgagag aattgacggt cgcacctgaa tccgctagtg ttcgtgtcaa
    59161 ataggttttt gataagccaa cgtcatcaaa agaacgcttg aatgtttctc ctggaagaat
    59221 gatggacaga tactgttctc caacgatcag attgattccg atacagctca aggcagtaaa
    59281 cccgactaaa cgagaaggtc ggtccatctt ttcttttaat tcttgaacga tggtatcaac
    59341 gatcagatat ttgatcagta acccacctaa tgccaatgca agtaaaatca aactagcaga
    59401 acctaacata ctcatgatcc cgcctcgaga aagcagttca tctagctttg gatcaggtgt
    59461 atttgcgaca taaccattca tcaagatgtc tgctgctttt ccgaccgtca aacttttctc
    59521 atggataaaa gaaagaacga gagcagctgt cgatcccgct agtaatgtag ggatagccgg
    59581 aatttttttg attgcccccc caaataaaat aactagtgga atcaatgtcc aaggagagat
    59641 ccaaaagctc gctttcatgt tttggatcat ttcattgata ctagctagat cggctgaatg
    59701 actgttatgt cctaatatcg tataaaaaat caacgaaagg acgaatgctg ggaaatccgt
    59761 ataacgcata ccagcgatat gttcgaatag attaactcct ccaatacccg ctgccaagtt
    59821 ggtcgtatct gataaaggcg agcagttatt ccctaaaaag gcaccggaaa cgactgcgcc
    59881 tgtagtcatt gcatcattga atccaagtaa atgaccgatc cccaaaaaag caattccaat
    59941 cgtcgaaatc gttgtaaatg aactgccgac agtcactccg acaattgcac aaacaagaaa
    60001 tgctgtcggt aaaaagaaac gcacagaaac aagtttaaat ccataaacca taatcgtagg
    60061 gatggttcca gataaaatcc atgaagcaac taagacacca atcaaaagaa aaatcaggat
    60121 tggaatgatc cctggagtga ttcctttgat gatcccgtca tggatctcat cccatgtgaa
    60181 ctttttaaat ctgccataaa acaacaaaca catgaaagct gcaaggatcg ggatatgtgg
    60241 cgagagctgg aatccgatga tcagtactcc taggagtgcc aacaacacga tcaatacagc
    60301 gatactttct tttaaagaaa tatttttatt catcgtcgat ctcctatttc aaatgaaaag
    60361 gccctgccat gaaaaaacta ttcacttggc aggacctcct actaaatcag cgacaaaacg
    60421 tcggctgaaa gacttatttt tttatttttc ttctaaactt ttttctacct caatccccca
    60481 agaatctttc ttgaagtgat tgatgatcaa tggaataacg aacatgacca ctgtgatagc
    60541 tgctactaat aatgcatcac tcaatgtcgc tgtacctaat gtagcaaata cgacaacgat
    60601 gatagagaag atcagcatgc agctgactac ccaagctaat ccgtttccgt ttttaccaat
    60661 tcggtaagga cgttcgaatt ctggatgttt cttacgcatt ttaagcaatg aaacagcaat
    60721 caaaatgtac acgatacaat aaataacagt tgtagcattt gtaagtgtca agaatacagc
    60781 acttacgtca tccattacac cataaagcaa agcaaataat gaaataacga ttgattgtgt
    60841 tagaacgaca tttctagata cgccgtattt gttttcttta tggaatccga attttggtgg
    60901 aaggaatcct tcacgagcta cttggatgat tgtttttgat ggtccagtaa cccaagctga
    60961 taattgtaaa agcacaccaa taaataccat gaagctgaag atatttccga tcacttctgg
    61021 taagcctagg atttgacagt caatcaagat aggctgcgtg atatttgcta attccatttt
    61081 tccatctggt acgacatttg ctacaagcat cgcattgatg acattcaata aaactaatcc
    61141 gatcaaagcg ataaatacgc cttttgtata attttttgtt gcgtctttca aacgaggcat
    61201 gtaaacagaa ctcatttcga tcccaacaaa aataaatgtg ataccagcta agtatttcaa
    61261 tgaatcaata tgttccaagt cagggaaagc ttttgaccat gaaaagtcac ctagatagcc
    61321 gtttgagact agtccgactt tgaaggctgc caaaacacct aaaacgaaca tgatgactac
    61381 tgggatatat acccctagcc atacaccgat gttcccgccg acttttgcca tatcgaattt
    61441 caagttcaag atcgtgatga tccagtagat aactaaaata caacctaaaa cgaaccagtg
    61501 attattgcca agttctacat taccaaatgt gtttcctaac aatggtccta aagtagaagc
    61561 gaccattacc atacctggga acatttgcac ccataatagc catgcaacga caaatcccca
    61621 tttgctgccc agtgctgttt ttacccataa ctgaggaccg ccttcttgag gaagcatcgt
    61681 ggaaagctca ccggaaacca ttgatatcgg accggcaaaa aacaaaactg caaaaattga
    61741 ataaaaaatc aatgtccaac cgactgcggc taacgtagga atactccgaa ccgttgcaca
    61801 cagtgccatt gtcagaccga taaaggtccc aagacccatt ttacttactt cagatttact
    61861 caccgttgac actctccttg attttttgga taaaataacg gtgtctttga agtagtttca
    61921 gcaataaacg aaacaattca gcgaatattt gaggggattc tttgagttta ttgcttgaag
    61981 ggtttcttca aagacaacct caaaacaaag ggatcgaaga gcttaggtct ccccttgctc
    62041 ttcagatcca aacatgcgtc agaaacagtt agtattcaag ctgttttcta acatgattac
    62101 cttgcttatt ttgcttcgct tgccaataat tggtctgcgt agattttttc caatttttct
    62161 tgaagagctg ctttgatttt ttcagcatat tcttcaaagt gttcttcttt gttcatgtat
    62221 ggtgtcatta cgctggcacg caatacagtt acttttccgg cacggttcca ttcttcatct
    62281 gagaatccta gttggttcac aaattgtaat gggctgttgc catagtctgg gatagcaaag
    62341 tcagtatgtg aagttaagaa ctcattaccg taaatgcttc ctttcacgta tgaagaataa
    62401 tcatatacgt catggttcaa tttattcatt gcaactaagt catcgttgcc tttttctttg
    62461 aatacatagt ctaccatatt gaaatctgga taagttaatg gatgaacttc gatttctttg
    62521 tctcctactt taaatgacaa gtcattcaag aagttgtaga aacgatgtgc accttcgatt
    62581 gatgcaccca ttaatttacc atatcctgtg acattcaaag gtaagacatg gtgtgctgcc
    62641 caaacgctgg ctgctgttgc ccctgctttt gaaccttcca agatataagc cccaagtaat
    62701 gctgggatat ctgcgccttt ttcaaataca tatgtcgcaa agtaagagat aacgtctctc
    62761 atgcggatat ctttgatgac gataccaccg gcagaatatg gaacatagcc cattttatgt
    62821 gggtcaatcg ttacagattc tgcttcttcg attgctttat atgcactgtg tacttcttct
    62881 aagatataat ctttgttttc tgtaaagaca ttgtatttgt aatgaacatc ttttaaatct
    62941 tcaaatggaa tgaagttgtt gtcttcgtct aagaaaatcg cacggccgta tccgccataa
    63001 gcagcatcta cgtgtaagta gaagtaaata ccgtcttttt ctaatacgcg acgcaatgca
    63061 acgattttat cgataccgtc gattgctcct tcttctgttg atcctacaac accgactaca
    63121 cctaggattg gtgttttttc agctgctaga ccacgaacga ttttttcaag ttcgttgatg
    63181 tccatgcgat agttgtggtc aactggcact ggaatcactt ggtctaaacc gataccgatg
    63241 atgtcggcag cttttagcca tgaatagtgt ttagtttgtg gaaccaacca ttttcctaat
    63301 ttttctaaat gttttccgct acgtgctgag tgagctttga tttcgtcgat tttttctggg
    63361 acgctgtcta acagattcat gatttcttct gttgacaagt tcattaattc ccaatcgctt
    63421 tttccagcaa ctagttcagg tgtcacttct ttcattgcaa gcggcaatga tttgatgttg
    63481 cgggcatacc aaagtccttc taagtttgct aaagaaccat cagcaacgat gtggccccag
    63541 ccatctttat agctcattaa ttttgcaaat tccattccta cttcttcttc catttggctt
    63601 gttgcaggag aagattcata ggcaacgtta ttaccattcc atagcatagc aaaattgtac
    63661 gctagtaaag atggcatcaa tgtttcagag ttcatatgac cccagtaacg tccggcatta
    63721 tgccaaggaa cggaatgggt acgcatccgc gctgaaattt ctgatagaac atcctttgtt
    63781 ttattcacag tgtgttcaaa actagcactg cttttttctt ctggcgtaat aataggcatg
    63841 tcttgaggca tgtagttttg acgccagcct aaatgttcat ccaccaattc attcaacaaa
    63901 gctttataga tttggccgtt ctcagctttg tccccaatga atagggcatt taggtttaga
    63961 tctttcgaca atgattcact catagtgaaa ccctcctaaa aaaatattta aattttgtcg
    64021 agccctacat tacacctgtt cttctattct gtaaactaag ttttggcctg atttttaatc
    64081 atattttaat atgtatcaat tatccgaatt gttcacactt ttgaaataat taaaaaaaac
    64141 gagggaattt acaaatgtaa actccctcgc aaagattgta ttaacgacaa ccagcgttct
    64201 attttaggat gggtttcaaa attggaatgt tgtaatagta tttccctttt tcaaggtaaa
    64261 aacttactaa attctaatca attactttgg ccaaaaagta atttttctta ccttttcgaa
    64321 taaccacaaa cttaccatcg aattcatcgg atggatttat gacaaaattt aaatcggtaa
    64381 cgcggtcacc gttaatactt atagctccgt ttgaaacatc ttcacgtgct tgacgtttag
    64441 atggttcaat ttttgtggaa acaagcagtt ccacgatatt ttcaggtgtg cttgaaattt
    64501 caacgttcgg cattttaccg aatccttgcg cgatttcttc cgcatttagt tctttaatat
    64561 ttccagagaa caatgcttgc gttatttttt gcgcttcttt caagtcttct tcactatgca
    64621 caaatcttgt aacttcttct gccaaacggc gttgtgcttc acgtttctca ggttctgtct
    64681 ctactttttt cgctaagtct tcgatttctt cttgagacaa gaaagtaaag aatttcaagt
    64741 atttcactac atcgcgatca tcttgattca accagaattg gtagaactca aatggtgaag
    64801 ttttcttcgg atcaagccaa acggcaccgc ctgcagtttt tccgaatttc gttccgtcag
    64861 ccttcagcat caatgggatc gttaaaccga acgcttctgc ttctggtcct tctaattttc
    64921 ggattaggtc taatccagct gtgatatttc cccattggtc tgctccaccg atttgcaatt
    64981 gcacatcgta tgttttatgc agatgcaaaa agtcgataga ttgcaggatt tggtaagtaa
    65041 actctgtaaa tgagattcct acttccaaac ggctggcaac gatatctttt gctaacatcg
    65101 tgttgatgtt gaagttttta ccgtagtctc tcaaaaattc aagtaaagag atttttgaca
    65161 accagtcgta gttgttcaca aatgtgatat ttgcatcttt accaaacaat tttctcattt
    65221 gattggaaag tgcgtccacg ttatgttgca cttgttccat cgtttgcaac acacgttctg
    65281 ttttgcggcc gcttgggtcg ccgatcgttc ctgttccgcc gccaatcaga atatatggat
    65341 gatggcctgc caattggaat cttttcatca tcataaatgg aatcaaatgt ccgatatgca
    65401 tcgaatcacc agttggatca acgccgcaat aaagtgaaat gctcttttct tcaacgagct
    65461 cgcgaagtct ttcttcgttt gtttgctgat taatggcgtc gcgccaagtt aattcatcaa
    65521 taatattcat tttactgacc tccgtgtagt ttttttatga aagcaggaac ctgctagcat
    65581 gtgtaattaa ttatttcttc aggataggat aggaaataaa aaaagtccct tggcatataa
    65641 gccaagggac gaaatctcgc ggtaccaccc aatttgtatg tctagatatc caacatacct
    65701 ctcgaacatt taacgctgtc gcgtggcaat cactgccaaa gactcctgaa atgtaattcg
    65761 ctttaaagca tgtactggtt tgcagcgacc accagctttc tgaaacattg gctttaacaa
    65821 ctacttcgtt tcgatcttcg tcttgttatt attaatatgt gtctatcata gagcgcaact
    65881 ttttctttgt caacagaaaa aggatatttt ttttaaattc ctcatttttt cttttttgaa
    65941 tccgctctaa ttccgaacat tttattttca attattgtta aaagaaataa ctttttctgt
    66001 attttttctg caatttatcg agaagtcact ttactttgcc tcttattctt tcttcgttac
    66061 aataaggaat gggacaagta aaaaaatgaa aaagcaaggg acgaggtata tggaaaaaaa
    66121 gcaaaaaatt attttaggag ccacttctgc tttaactatc gttattttaa ttctaagttt
    66181 gttttttgtc atccgatcat ctacaaaaaa agaaccaatc attcctgaaa aaaaaataac
    66241 agaaaaagca aaagaaccga cagatgataa gcaacaacta gttactcaag caaaaaatct
    66301 ggctcaaagc tacgattatg accaagcgat tgcgttattg gaaaaagata gtaagaacgg
    66361 atctactgaa acacaacagc ttcttcaaac actaaaaaag gagaaagaac aactgatcga
    66421 ctggtctgat cctactcaga tctctcatgt ctttttccac agcttgatcg tggacccgca
    66481 aaaagccttc cactctcaac aggcacaagg atacaaagat tatatggtga ctgttgaaga
    66541 attcaaccgt tcgatcgaac aactttatca aaatgatttt gttttagtta atttgaatga
    66601 tttgatccaa aaagacgaac aaggaaatct ttcctttaac gggcttgcgc tacctgaagg
    66661 aaaaaaacca ttggttcttt cacaagatga tgtcagctat tatgaatata tggataattc
    66721 cggttttcct agccgattgg tcgtatcaaa taacaatgaa atcaaaaatg tctataaaga
    66781 gaaagataaa gaagaaatcg gtgactttga catggttcct ctgatcgatg cctttgtcaa
    66841 gaaacatcct gatttttctt atcgaggcgc aaaaggtacc ttggccctga caggttacaa
    66901 cggagtttta ggttatcgaa catcaaaatc tcaatatgga gacaatgaaa aaacaaaaaa
    66961 agagattaaa gaagctaaaa aagtggcgga acagttgaag aaagacggct ggagctttgc
    67021 ttcccataca tggggtcact tgaatatgac tcaagcttca ctagaagatg taaaaaaaga
    67081 caatgaattg tggcagcaag aagttgcacc tatcctagga aaaacaaata ttctgattta
    67141 tccatttggt gcagacatca gcgactggca gccttattca gcagaaaatc aaaaatttgc
    67201 ttacttgaag agtcaaggtt tcgatatttt ctgtaatgtc gatgcttcta caccagcttg
    67261 gggacaatta ggtacggatt tctatcgaaa tgctcggatc aatatcgatg gcattcgttt
    67321 tcaatctgat ttagacggaa aaaatcctat tcttgatcca ttcatcaatg tccatgatgt
    67381 ctatgacaaa gaagcacgcg aatctgcttg attttacgtc atttatgaac caaaaaagcc
    67441 gaactgacat tttatcgtca gtttcggctt ttttatgctt tatttcttct tcggtgcgat
    67501 tcccttgatc aggacttctg tccattctgc tgtatatgga agcagttttg cgtatatctc
    67561 cggcttagaa attccttcta ccattgcttg aaaatagagg atcaagaact catccgaata
    67621 tttcaaatcc acttttcctt ctttccgccc tcgatggaaa agatcaagca tgatactgaa
    67681 actttcttgc gtatacgcat ccatcgcgtc agaaaattct tttttttctt tttttgtata
    67741 gtatgtcatc aaatcgatat aaaattgttt gttcactcga ttgagataac ggattttgtt
    67801 ttcactcatg gcaatcagtg tttcttcaaa tggaagattt tccgcaatca acgctttcgc
    67861 ttcttgccct aaatcattca taaatgcaaa aaatacttca taaatcagcc cttccttgct
    67921 gccaaaatat ttaaaaatcg tcgctttact tacctgagcg ttctcagcta ccttatccat
    67981 tgtcactcca gcggttccta tgtctgtgtt catcaagtca aaagctgctt gtaaaatctc
    68041 tttctttttc tcttgtgctc tcttttcaaa accgttcatt tgtagagcct cccgtaattt
    68101 taaactttta taatatatat agttcaaatt tattctgagg tttattataa ctatctattg
    68161 agaaagtatc aagctagcaa ttccttgttt atgaattcat tgacagaaat ttataaaaag
    68221 aatattatgt actaaaataa taaaaaaagt tttataaaaa ggtataaaac ctttttatta
    68281 aactatttaa tcttgtttag tatatatttt tagaaaaagg agtggggttt ttatggcaga
    68341 aatcgtcaaa gtcacacatt tgaaaaagag ctttggtcgt ttcgaagctt taaaagatgt
    68401 ctctttcacc gtacattcag gtgaagtcgt tggatttatc ggaccaaatg gttctggaaa
    68461 atccacgacg attcggacac ttttagggat tataaaaaaa gacggcggaa cggcaacgat
    68521 ttttgggcaa gatgtatggg aagatagttt atctatccat gaacgtcttt cttatgttcc
    68581 aggcgatatc tctttgtggg gaaatttgac tggaggagcg atcatcgatc tcttcatgaa
    68641 gcttcatgga aaagggagtg ctcaaaaaaa agatgaactg attcaacgtt tctctcttga
    68701 tcctaagaaa aaagcaaaaa actattcaaa aggaaatcgc caaaaagtgg gcttgattgc
    68761 tgctttggct gttgattcgg atctctattt atttgatgag cctacttctg gtttagatcc
    68821 tttgatggaa acgattttcc aagaagaagt tgaaaaattg aaagaacagg gcaaatccat
    68881 cctactttct tctcatattc tcagtgaagt cgagcgacta gcggataaag tcgtgatcat
    68941 acgcgaagga acactcgatg aagaaggaac actcgatgaa ttgcgtcatt tgactcgttc
    69001 gaccatcact atagaaacag aagcagagaa tagtcactta gcacaattga acggtgtcca
    69061 tgactttgtg caaaaaggtc acctcgctca atttgctgct gataacgaag ctttcgatgt
    69121 gattttagct gaagcgtcca aattaggtgt gaaaaaattt gaatctattc caccgactct
    69181 tgaggattta ttcatgagac attatgaagg atgaggagga agcgaaatga ctgataaatt
    69241 ttcacgctgg tccacccttt tttctcaaaa ccttagccgt gactggaaaa agatcctgat
    69301 atggctagtc ggtgtcactg ctttttctgg tggttttgtc cctgcgtttg aagaattgac
    69361 ttccggacaa ggattagctg gattatacga aaccttgcaa aacccggcaa tgattgcaat
    69421 ggtcggacca actccagctt cgaatgctgc tgagtatact ttaggagcga tgtatgcaaa
    69481 tgaaatgttg cttttttgca gtttgtttgc tgctgtgatc tctgttttac acgtggtgag
    69541 ccaaacgaga aaaaaagaag aacttggtct gattgaactt attcgttcct ttcgagtcgg
    69601 acgtcaagca aactctttag ctgtcatcgc tgaaagtatt ctgatcaatt tgcttttagc
    69661 aatatcgatc agttgtacga tgattttttt ccaagcggat tcgatcactg cagaaggtgc
    69721 ttggcttttt gggctatctg taggtagtat ggggattttg gggagtgccg ttgcactgtt
    69781 tgctgcacaa ctgatgccaa gttcagccag ttcgaatggt ctgtctttag cgtttttagg
    69841 tggactttac cttctacgag caataacaga tgtagctgat tcctcccttt cgatgtggaa
    69901 tcctttaggc tggtcttatt tgacctatcc atttacaact aacaactgga ttcctttgct
    69961 ttatttgttt ttgttcagtc taatcttgat aatcagttca tttgttttag aggaacatcg
    70021 agatttgaac accggctata tccccgaacg tgaaggaaaa ccgtatgcta aacgttctct
    70081 tttatccttg cgtggtttat tattctatct aaataaaggg atgacaatca gttggcttat
    70141 cacttttggt ttgatggggg cggcatacgg ctctatctac gggaatatgc aacagttttt
    70201 agaaagtaac gacttaatga aacagatgtt tatccaatct ggcgtctcaa ttgaaaaaaa
    70261 ctttacaagc acgatcatgg tcgtcttgat ttgtctagct gctattttac caattgctag
    70321 catcaatcgt ttatttacag aagaaaccca cggacgtatg aatcagctgt tcgcaacaaa
    70381 aatttctagg aaccaacttt attggaccaa tatgttacta gctattctat ttgctatatt
    70441 ggcaatcctg attagttctg gtgcactagg gctttctgct ttggcttcga tgaaggatgc
    70501 gaaagacatg gatctattga tgtttcttaa agccgggttc aatttcctgc ctgccgtttt
    70561 agtgtttatc ggaattagtg gctttctatt cggatggctt cctcgaatcg gtaaacttgc
    70621 ttatatctac ttagcttact cctttgcact caattacttt ggcggaattt tggatttacc
    70681 agaatggttt tccaaaacag ctgtccctag ctgggttcca cggatgccaa acgattcttt
    70741 tgatctttcc gtgttcctta cgttatcatg tatcagcctt gcactgttcg tccttggttc
    70801 tattggttat aggcgaagag acttactaga aggataataa aaaagcatgt acaatttcat
    70861 gctagcctcc aaatctgggt caaaaatcca acatttggag gtcatttttt tatttacaag
    70921 tcttctaact gctctttatc aatgatccag acttctcgat tctttctttt tacataacca
    70981 ttctcctcta ataatttaaa ttttctagat aaagtttcgg gggtcgtacc gataaatgca
    71041 gcaagttctt tcattttcat tggcaggaaa aagtgatcct tccctgtcgc tttagctaag
    71101 tcaagcagat agacagcgac tcgttcttct actttttcca tcatcaaaaa agttgtctgc
    71161 tgctgtactt ttgttaattt ctcaacgttg attgctaaaa gtttcagact aagggcggga
    71221 taatgcagta agagttccga gaaatctttt tgtctcaata tgcaaatttc cgttttttct
    71281 agtgcttcgc caaataaaga ctcatttgga acgcctagca actggctctc tccctcatat
    71341 ccgcccggct ctattactcg taaaagctgt tcctttccgc tagcagataa ttgatacact
    71401 ttcatactgc cggaggccac gatcactaat tgaggttctc cattcggcgc aagaatctgt
    71461 tctccttttt ccacatagtg atgtttgaca agtgcgtgta ctttttcttg atcttgctgt
    71521 actaagtgat tgaacaaagg aacaaatgaa acacataaat gatcatgact cataatctct
    71581 cctccttcta aacagctatt tctaataaag aaacataact tcccttattc caactgataa
    71641 ttttctttta tcatagcata aacgtcaagc gaaccagata ccagcaaata gagtggattt
    71701 ctttttgtcc cttgctttat tttccttctt ttgagacctt aagtaattga ttcctatcaa
    71761 ttttttaaat atttactttt tgaataatca aaaacaaacc ttcactcgca agccgagagt
    71821 aaggtttgtt tttcgcattg cttatttcgt gttgtctatt gaaatttttc cgcacctagc
    71881 ttttgatata gtggtgctag ttcttgtaag gtttcagcgg ttatttgctc aacgtgttcg
    71941 atttcttgcg gatagaagaa tagtttaccc atttgaaagg ttcggtctct catcgctttt
    72001 ttcaactctt cttgtttctt tgtatcggac aaatagtcag acaaagctaa gtggtcgtca
    72061 agttcatgtt ctggttgcgg ccatatgtaa taatccttga tatcgactgt tttgctccac
    72121 tctttcagta tcgcaagcaa ctggttatac gctgcaatgc taccatatcg ttcttcgctt
    72181 ttataatgtt ggtacatcaa atagatttgg tattgttttt gattcaacaa aactgctaga
    72241 caggcattct cgtcttgacg agattcgctt cggtacgcgc tccaaaaatg actgcgtaaa
    72301 ttccagccat tcgtccagct ttcgatttta ggtttgctca tgccgtacat atcaggcaaa
    72361 agtgcagctg tctggagctg aatctctttc catttttgcc agtgttcttt atattctttc
    72421 ttgatttgtt caatcttatc tgttggatgg ctctctttga gctgtttgaa tgcgtagatt
    72481 ggtcgatcaa acaactgata atctgatttt ccaagttcca ttgtttcgcc tctttccgtt
    72541 atcttttgta ttctacctga attttgcgat atggacaaat agaggttgtg aaaaagctgt
    72601 tctgcatcaa gtcataagaa cagccaaaca aaaaactgcg atgagcatca ccgcagtttt
    72661 attgaactta aggttgattg atcacatcta aaagcgttga aaataaaatg gcactagaag
    72721 cagctcctgg gtcgatatgt ccgattgcac gctctcctag ataggaagct cgtccttttt
    72781 tagctaccaa gactttggtg tgttcttttg cctggtcaat gatttctttt gttaaattcc
    72841 ctgccttcaa tgcctctgtg actggcagcc aaacatctaa catcgtttta tcgtcagctt
    72901 ctgcctttcc tcggctttga atacccgctg ttccctgctc gatcacttcg cctaattttt
    72961 cttgagaatc aatagaatcg acctcttttg tcgctttcat catgttcatg aatgcagatc
    73021 cgtataacgg accagaagcc ccaccgactt tcgatatcat cgccatagag aatactttga
    73081 atgtatcgct gattgtttca ggatgatcgg cttgaaaagc ttcttcataa gctgaaaccc
    73141 ctcttcccat gttatttcca tggtctccat cgccaatcgg ggtatctaga tcactgagat
    73201 attgtttgtt ctgattgatt gcctctgcaa attgcgtgag ccattcttgg atttcttgaa
    73261 cggttagttt catattatca cccttttatc ttgagatttt ttaccatgaa atcgtctgta
    73321 cattgctttc taaagaatct ttccattgtg tctccgtcaa gtcgatcagt gttagagaaa
    73381 gaccttgcat atctagtgag gtcataaaat taccgacttt tcggaaaact acattgacgc
    73441 ctctttcttc caacagagct aaaacatcat tcatgaacac gaactgttcc atcaaaggcg
    73501 tcccgcccat tccgttgact agtacacctg cttcttttgg taaattcgag taatcatcta
    73561 aaatcttagt aacaagttcc tttgccagta ctttagaagg ctgcatcttt tctcgacgat
    73621 agcctggctc accgtggata ccaacaccat actcgatctc atcttctcct agttcgaatc
    73681 ctggtttccc tacttctggt acagttgctg ctcgaagcgc tagaccgatc gtcttagttg
    73741 cctgtacgac cttttctccc agttctttca actcagctag ggaagcaccg tttcttgctg
    73801 catcgcccac gattttgtgt acaaggacag tacctgcaac tccgcgtttt ccagcagtat
    73861 acgtactatt ttcaacggcg atatcatcat ctacgatcac actttctact tcaatatcgt
    73921 ccatagcagc taattccttt gccatatcga agttcaatgc atcccctgta taattcttaa
    73981 cgatcagcaa aactcctttt ccactatcgg cttccttaat agccgtctga atctgatctg
    74041 gagttggaga agtaaacaca tcaccaagca ccgctgcact taacatccct tcccctacaa
    74101 acccggcatg ggatggttca tgtccgcttc ctccaccaga aaccaaacca acttgttccg
    74161 ctttctgatt cttggctaca acacgggaag attctacacg atgaacaagt tctggatagc
    74221 tttttactaa tccttccagc atttcttcga caatattgct tggatcattc atgatttttt
    74281 tcaatttatt cccatccttt ctttccggca ttttgtgctt ctgttaagat ttgctgagta
    74341 tcatctgtta taccggcagt gatggctgct gcaaaagctc cttccactaa aggcgcatcc
    74401 accagatgat aacgttgctg ctgctcttct tccaacatgt cttttgccag ttccgagttc
    74461 aataccgcgc taccgatatc cgcaaaaaca aaataactag ctgcatcgct tgattcattg
    74521 actgcttcta cgattttcat cgggtctgat ccgatctcgc catttattcc gccgagagaa
    74581 aaaatttgca cctcttcgga tttttgcatc tgctcaatca tttctttaat accatctgtt
    74641 actttttgac tatgagaaat aagcaaaata ctttttttca caagaatctt cctttctttt
    74701 acatagttgc taacgattgg tttcgctttc attaatgaac aattcaagta tataataaat
    74761 ttctaatttt gggaaaatat gtgtagtgct tcttgaagtt tgttatactt taattaaaag
    74821 agaggagtga tcgaatgatt acaacaacaa cgaacagtgt agagggaaaa gccgtagtag
    74881 cttataaagg gatcgttttt ggtgaggtca ttactgggat ccatgcattt aaagacttgg
    74941 aggcggggtt gcgaaatatc tttggcggcc gctcaaaaag ctatgaaaac gaactaatgg
    75001 gtgcaagaga agaagcactt gccgaaatgg ccgaacgtgc aaaagcatta ggagctgatg
    75061 cgatcattgg cgtaaaaatg gactatgaag tattaggcag cgacaatggc atgctgatgg
    75121 tcacatgtag tggaacagct gtctccacga gataagtagg ggacatctgt tttaataact
    75181 aaagagagat gggacatgta gtggcactca agaattagat ttttcagtct aacttcgagg
    75241 tactctacct tcccatcctc tcttttttat ttctttttgc ggatgattcc gtaagctgct
    75301 gcgactaaca cacccgcccc tgctaaggaa taactgacag aagctgtttt ttcatttgtt
    75361 tttggcaaag tctgacttgt tgatttggat ggctctgtcg tttgctgaac tgtttctgtc
    75421 gtgcttccag aggtcactga tttttctcct gccgaatgca aaatatcttt tattcgttgg
    75481 aaactagctg ctaagtcagc tttttttgct tcaaaatcat tagcaactgt ttgtgcttcg
    75541 ctgatctttc cgtggattgc ttgactggcc gctgtgatat ccgcttgagt ttgacttagc
    75601 tttccagaag ctgtttgagc aacctgattt aattgctgat tgaattcttt tgcttgttgg
    75661 atcttcccgt caagatctgt ttggacagct tccgcttttg ctttcgcttc ggcaagcttt
    75721 ttctcaagtt gtgcacggtc atttcccact ttatttttaa gatcagctaa atcttgatcg
    75781 attgctgctt ggtcttcttt caatttgttt tttgcagctt ctaatgcttc ttcgagttct
    75841 tttcttttat tttccaagtc ttgcaaatga tcgatttggt cagaagaggt agaactattt
    75901 attgatgtat cttctgaaga tgaaatcgtt tctcctgaat tatctgtcgg tacattggaa
    75961 gtcatttctg tttccgaggc accgttttca gcaccagaag agcttatggt tgttgaaggt
    76021 tctgtttgag caggttcaga agtttccgca aaagctgtct gtcccaataa gaaaatactg
    76081 gaaacagcaa ttggccagat taacggcttc attctttttt tcatcacttt tcccatcctt
    76141 actacttttt aactaatttc ttattataag caaaaatgtt tggaagaacc ataaacacaa
    76201 caggctgttt gtctaaaaaa cggtgcatct tttatttttt tgtacgttcc gggcttttcc
    76261 atgtcccgct tccataacct aaaatcgttt tgactgctgg aatgaaagtc gtcacgatcg
    76321 ttatcgcgtt cactatccag aacaaaagca tatatagtgg tgcgaaaaga aggtatttca
    76381 attttcttcg tcgatcatct acaatcagcg aagctagcaa ctggaaaaat cctgcgacca
    76441 tttcaaagca gatgaacaca aaactgaatg taaaagtaat ataaatctgt tcgtagtttc
    76501 cttgatatac ataatgcccg attaatccag caaaaagaac tacggaaata caaaagaata
    76561 ttgaccagac aatactcaat gtttgatcaa tgaagatgat cgttcttcca atatgtttaa
    76621 aaggatggag catcactttt ttgaaattcg tcagccagac ttctgttcca ccttttgccc
    76681 aacgttttct gtgtcgatat agcatcttca acgtcacagg tacttccatg aaaaagataa
    76741 tctcaggagc aaaaacagat aaccagtcat ttaactgatg atcccaagca atactgatgt
    76801 cttctgtcgc tcgatcttgt ctgaatccgc ccacatcgat caaggcttct tttcgataca
    76861 tcgtatttgc tccactgtac gcataaagac caccgaaaac agctgactgt gttcgtttaa
    76921 tgatgcccac gatacttgag aattcaaccg tttgagattt tgcaatcaat ttggtacgat
    76981 tttggacatc catatttgca gtgaccgcag caatatgtct tgcaccaggg cgaatgaaat
    77041 aattcacata cctaatcagt gcatctggct ccggtaccgt gtctgcatca ttgcttaaaa
    77101 tcaatttccc ttttgcaaag gcaagtccga tattaaacgc gtgggctttt cctttatttt
    77161 tttcgatccg aaccacacga agattagcgt attttttcat cagtctagct aatatttctg
    77221 gtgtttggtc cgttgaacca tcgtctgtca ctaatacctc atagttgtga taattcaatt
    77281 tcgtcatcaa atactcgatt gtatattcga tcacaatttc ttcattatgg gctggcacca
    77341 tgatcgtgat gaacggttcg acactcaagg gaacatctac ccattctttt tgcttatgtt
    77401 tgtataaaaa cacataacac cagacaccaa taaaccatac aaatccgcct aaaatcggat
    77461 aagaaacgag aaaaaaatac ataatagaca aacaagtatc taagaataaa ttacctgtat
    77521 gggtgattga gatcatttca gttccacccc attttcttta tacaaatctg caatgaatgt
    77581 cgtatctaga tttttctctt cggagacaga ataaaaacgg accgtttctc tctcttcttt
    77641 tgttccaaaa cgttccgtat acacttcttc aagtaattct tgtctttttt tcaatttttc
    77701 atcttcatat tcaaaagatt tatttacttt tttgtataga tttcgattgt tccacctagt
    77761 caatacaaat gaggtaatca ggaaactgaa aaaaatcaat acaaaaaagc cgattaagaa
    77821 atacagcatt ttcttttctg ataaaaaagc gtacaaggaa aaatccttgg ctctttctgg
    77881 tctcgttaat gggaaaaaga cccatataaa aggaagaaca acgatcatcc atcctaaaag
    77941 agccactaac gtttgccaaa tctttaacca gtaattacct tttgaaaaat acttatcttt
    78001 caccaaaatt gatgcaactt cttctcttgt tacttttttc atatcctcac ctcacgtttt
    78061 tttgttacga cgtaggagtt taccatacat ttcatttctt ttcacataaa gtgttcctaa
    78121 atttttccta agaaaaagaa caaaacattt ctcatcagca aatgaaaaat attttgttct
    78181 ttttatcagt tgttcttttt attttttatc tcttaattat gttgctcttt ccaatacgac
    78241 aatgcttttc attcctctag atatcgccgt gtacatttgt ctgcgtcttc tttgctcatc
    78301 tccatcgttt tcaaatggat ataagattac atgatcaaat tccagtcctt ttgctagact
    78361 caaaggaatg atttggcagt tttctaatcc atatgaattt tttttaatcg attcttgtag
    78421 cgtttgggtt tcctcttctg tttgggtgat aactgccgct ttttctcctt ttagagaaac
    78481 caaaatctca gctagtattt tcaacaattc ttcagaggat cgaaaagagc ggtattcggg
    78541 ttcttttcct tgcggtcgga tcgatacgat gtcgatcttc tcttgatcca cagcataggt
    78601 tttgaaaagc tctgtgattg ctccgctaga acggtaacta tttctaagat cataccgatg
    78661 gatcggcaga tggtagtcgt cgaaacaacg cataatatca gcaaaagtag tggaagaatt
    78721 aaagattgcc tgattctcgt cacctactaa agtaaagtga gtttttggaa ataattcgat
    78781 caatagacct aattgtgcca gggtataatc ttgtacttca tcaatcaata gatagcgaaa
    78841 tgctggtaca tttacctttt caacaagtaa atgacgaata gctaatataa tgactgcttc
    78901 atcaacagta cgtgtgggtt gccaagcata agctgagcca tatcgttttt cataaattct
    78961 ttcaaacaac tgatgctcat ccacccatgc catttcttcg atttgtctgg ttatttttcg
    79021 atattttttt tgtaaaagtt tccttgcata agctacaata ctttgttccg aatcatcaga
    79081 aatcaattta ccgaataatt ccaactgcct atcttctgtc aacgagagta cctgattctg
    79141 cacagcagga ctttttgctt gtttcaaaag atgattcttc cattcgctca tcagtgcctt
    79201 tttcgttgct tgaagcctat cgatcaattt aggatgagca ggaactttct catatatttt
    79261 ttcgatcgtt tttttagaaa ggactgtttt tccattttta gtaagtccac ggataaaatt
    79321 cggatgatct aaaaacaaag gatctgattg ttttagatta tcgataaact ttttgctacg
    79381 caaacgttct gtctgctccg atacattttc tcccgtgata cgtttgaaat aagcctcttc
    79441 accttctatt tcctctgctg aaagctgtga aaccagctgt atgatggtta gattcagtgg
    79501 atttctttca cctaatgatg gtaggacatt cgaaatatag tcgataaacc gatgattagg
    79561 tgatagaatc agcaagtcat cggatgtcat cgtatctcga tataaataga gcagatacgc
    79621 aatacgctgc atgatagcag aggttttacc acttcctgct acgccattta ccaagatatt
    79681 ttgacttttc acatcccgaa tgattgtgtt ttgttccgat tggatcgtag cagtgatagc
    79741 cttcatttca ttggtctcat cttggcctaa gacttctaat aatacgtcat cttgaatagc
    79801 tacagacgta tcaaaaaact tgaccaaacg atctctttcc aaaagaaatt gtcgtctttc
    79861 tttaataacc gtatcgattt tattttgacg aaccgtataa gaagatggac caatcatgtt
    79921 attgtaaaaa agttcggcaa ttggtgaacg ccaatcataa acaagatttt catctgcatc
    79981 atctgtgaaa tttgctgtac cgatataaaa ggattctttt ggctcattat ccagaaagtc
    80041 tacaacgact tttccaaagt agggactttt caataaacgt tcgatctttg ctagctgatt
    80101 ttctgcgttt ttctgtttga tattcatctg atcgatttct ctatttttca tttcaatcat
    80161 cgaaaaagta tccaggttat ctaagtaact gtcaaaattc aatcgaagct cgccagccat
    80221 ttctttcaac gactggtttc cttctatttt tgccgtatcc atcgattccc gcagttcgtt
    80281 ttttttatca attagctttt gatacacgtt cttcagatat gcttcttcta actgtttttg
    80341 tgtgttgacc atacacattc tcctcttttt ttaaagtggt ggaaccgatt agtttacctc
    80401 ttttaagagt cgaatgcaag ctttttaacg agtatcattt gttaggatat atcagttctt
    80461 caggtcggac agtttgatat aaagaaaatg caccatcaag gttttttacg tggtagccag
    80521 actgcttcaa gattctttcc gctagatagc tacgtaatcc gctatgacag ctgacgatat
    80581 attcttgttg gctgtctaat tcatttaatc ggtcccgaag ttcattcaaa gggatatgtt
    80641 tggcttcctt gaatttgccc ttttccaatt cttctgctgt tcgcacatct aataaaattt
    80701 ttccttttgc tagttctgtc ggcaattcat gccattgtac ggtttcacta agcccttctg
    80761 caatgttcat tgctgcatat cctagcatgt ttactggatc tttggctgag ccaaatggcg
    80821 gtgcatacgt caattctaat tccggtaaat cgaagatcgt caaatgccct ttgattgctg
    80881 ttgctaaaat atcgatccgt ttatccacac cttttgcccc aacaccttgt gcaccataaa
    80941 tctcgccagt ctctggatgg aagatcaact tcagtaaaat atctgttgct tctggataat
    81001 agcttgcatg atcttttcct gatacatgaa tgacttcaaa agacagttcg ttttctttgg
    81061 ctgttctttc acttaaaccg gtggatgcgg cagtcattcc aaacgttcgt ataattgctg
    81121 ttccaatacc gccttgattt cttcgagaaa ttccggaaat cgtgtccgca acttgtctac
    81181 cttgacgatt ggctggtgaa gcaagagaaa taagtgcatc ttttccagtg atttgctgtt
    81241 ttacaacgat agcatccccc accgcataaa tatcaggaat attcgtttga taacgttcgt
    81301 ctactaggat acccccgcgt atgcctaatt cgattccagc ggtcactgct agctggttat
    81361 caggacgaac accaatggat aaaataacta aatcagcagg gatggtacgc ccatcatcca
    81421 gtcgcaatcg tttcccgtgg tcttcaaatc cagcgacagc atgagaagta atcacttcta
    81481 ctccttgatg agacaattct ttttctataa aagctgccat ttcttcatcc agtgttggaa
    81541 gaatatgtgg tgcttgttcg atcaccatga cttctaaacc tcttcttttt aggttttctg
    81601 ccatttccaa tccgatgaat cctgcaccaa cgatcacggc acgctttggc tgtttttcta
    81661 atgcatgcat cactttatcg atatctggta catttctaat agaaaaaaca ttgtcggctt
    81721 ctgttatccc tggaatcgat ggaacaactg gttttgcacc aggagataaa accaatgcat
    81781 cgtactgttc aatgtgtttt tgaccctcat gtacgacttc taccgttttg ttttcgggaa
    81841 agatggctgt cacttcatga tttggacgca catctaaaca aaaacgagcg gataatgatt
    81901 ctggcgtttg gacaagaaga ttttcccgtt cactgatttc tcccgaaaga taataaggca
    81961 agccgcaatt tgcaaaagaa acatacggtc ctttttcaaa aacaacgatt tctgcatctt
    82021 ccattaatcg gcgaagccgt gtagcagcag acattccacc tgctacacct ccgacaataa
    82081 cgattttcat cttatttccc tcctactgtt cgatcaaacc attgattcat gccaccacgt
    82141 acattcacta catcataacc agatttcttt aattctttag ctgcttgttt actcctcatc
    82201 cctgattgac aaatcacata gacggtcttt tctttgtctc catcatatct gtttatactt
    82261 tgtaatggaa catttttagc tccttttata tgcccgcccc ggtattctgc aggtgttcta
    82321 acatcaagta acgctggatt ttccaaaagc ttttccttta attctgggac tgttatcgat
    82381 tttattgatt gaaatagcaa attcattttc tgcacctcat ttacattttt ataaatacct
    82441 catggggtat attaaacagg agctttatta atatgtcaac caaaatgact cttcattttt
    82501 ttcactactt tttatatttg cataaaatac cgataagggg tattatctaa ttataaaaat
    82561 agaggagaat gtgttatgga gattgataat aaaaatatcc tgaaccgttt gaaacgtaca
    82621 gagggacaaa ttcgtggtat ccaaaaaatg atcgaagacg aaaaagaatg tatggatgtc
    82681 atcactcaat taagcgctgt tcgttctagt attgatcgcg taatgggcat gatcgttgca
    82741 gataatctga aaaattgctt tgagaaacct gatagtaatc cagaagaaca agcggctaaa
    82801 ctagaacaag cgatcaagat gattattaaa aagtaaagga atgatttcaa tgaaagtcgt
    82861 tttccatatt gatgaattag aaaaatggtc agaaacaggg aaaaacgtaa aaaacctgat
    82921 aaaagcttct ccagaaacga cgattgtcgt atctgtaaat ggcatcgcga ttaccggtta
    82981 tctagactct gccaatgcag aattcttaga tttgcaagga gtcgtttttc acgcttgcgc
    83041 caatgctatg cgtgctaatc atatttcaga aagttcttta cctgaacaag taatcgttgt
    83101 accagcaggt gtccttgatt tagttgaact gcaatcccaa ggttatgctt atatcaagcc
    83161 ttagtcagta gaggttgcga cagaagtgat cagcttcaag aaatttcggc ttatttttcg
    83221 aagaagctac ttctggaaaa ccgtgaatca aaggttgtga aaacgctgtc ctgcatcaag
    83281 taataagaac agccaaacaa aaatagcttc tcatattttt cgtttggttg ggcttattac
    83341 cgcagatgca agcttttgaa caccgtttaa ggaagaggtt gtgaaatcag cggtttgacc
    83401 taagtattag agaaaaaaac gggaaaatag ctcctcatat tttgccgttt tttgagctaa
    83461 taccgaaggt cagctgattg aacaccgttt aaggaagaga ttgtgaaaaa gtcgcttagt
    83521 ttcgagtatt aagaaaaatt ttgtgaaaat ggatttctac atttttgcat aaatttggct
    83581 taataccgag aaactggctt ttgaataccg tttaaggaag aggttgtaaa atcagcggtt
    83641 tgacctgagt attagagaaa caaacggaaa aatagctgct catattttgc cgttttttga
    83701 gctaataccg aaggtcagct gattgaacac cgtttaccaa gaggttgtga gcctaccatt
    83761 taactccgaa tcacaagaaa taatttcaaa aacaacttct cctgtttttg gttcggttgg
    83821 cttattatcg agaagtagct actgggagtc tatttatcaa aaaaaggaat gcgacatagg
    83881 tcacacccct ttttt
//
LOCUS       DAZUMM010000002        78983 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000002 DAZUMM010000000
VERSION     DAZUMM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.002"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..1391
                     /locus_tag="ACG8E6_000074"
     CDS             51..1391
                     /locus_tag="ACG8E6_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5060535.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1609..2052
                     /locus_tag="ACG8E6_000075"
     CDS             1609..2052
                     /locus_tag="ACG8E6_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFS5060536.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2317..3735)
                     /gene="nhaC"
                     /locus_tag="ACG8E6_000076"
     CDS             complement(2317..3735)
                     /gene="nhaC"
                     /locus_tag="ACG8E6_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS5060537.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4138..5361)
                     /locus_tag="ACG8E6_000077"
     CDS             complement(4138..5361)
                     /locus_tag="ACG8E6_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS5060538.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVEQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5392..6231)
                     /locus_tag="ACG8E6_000078"
     CDS             complement(5392..6231)
                     /locus_tag="ACG8E6_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5060539.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETTVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDEKNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6248..6928)
                     /locus_tag="ACG8E6_000079"
     CDS             complement(6248..6928)
                     /locus_tag="ACG8E6_000079"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS5060540.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(6930..8003)
                     /locus_tag="ACG8E6_000080"
     CDS             complement(6930..8003)
                     /locus_tag="ACG8E6_000080"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS5060541.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IIEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKINILQKNELDSRIKVIEGGR"
     misc_binding    complement(8059..8297)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8339..8572)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8649..9320)
                     /locus_tag="ACG8E6_000081"
     CDS             complement(8649..9320)
                     /locus_tag="ACG8E6_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5060542.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYFDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(9407..12055)
                     /gene="mgtA"
                     /locus_tag="ACG8E6_000082"
     CDS             complement(9407..12055)
                     /gene="mgtA"
                     /locus_tag="ACG8E6_000082"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFS5060543.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLTFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASMFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGKFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNVTFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIHRFQELL"
     regulatory      complement(12397..12571)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            12744..13181
                     /gene="ndk"
                     /locus_tag="ACG8E6_000083"
     CDS             12744..13181
                     /gene="ndk"
                     /locus_tag="ACG8E6_000083"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFS5060544.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAVDAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13203..14069)
                     /locus_tag="ACG8E6_000084"
     CDS             complement(13203..14069)
                     /locus_tag="ACG8E6_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5060545.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICSKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLSLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEKKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14229..16322)
                     /locus_tag="ACG8E6_000085"
     CDS             complement(14229..16322)
                     /locus_tag="ACG8E6_000085"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5060546.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWMKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVLYWLDIATFIGILVWLTIKKIPIKSQPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFSESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSAIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWNEFDN
                     AQMQRVPLMIHIPGYTKGSVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSPQHD
                     QVVAFRNGNFVTPKYTILGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKSALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNIAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKDNQ"
     gene            complement(16798..17421)
                     /locus_tag="ACG8E6_000086"
     CDS             complement(16798..17421)
                     /locus_tag="ACG8E6_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060547.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(17580..18977)
                     /locus_tag="ACG8E6_000087"
     CDS             complement(17580..18977)
                     /locus_tag="ACG8E6_000087"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5060548.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHPEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIKF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19009..19980)
                     /locus_tag="ACG8E6_000088"
     CDS             complement(19009..19980)
                     /locus_tag="ACG8E6_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5060549.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKELLEKGVTAIFVSNYNMSIGAIECFNQEGIRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGEQAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20354..20779
                     /locus_tag="ACG8E6_000089"
     CDS             20354..20779
                     /locus_tag="ACG8E6_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060550.1"
                     /translation="MKKLIVLFILSAGLLTLTGCNLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQETDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATITFTKIGNYRLKDEDGNIYSL"
     gene            complement(21267..21968)
                     /locus_tag="ACG8E6_000090"
     CDS             complement(21267..21968)
                     /locus_tag="ACG8E6_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS5060551.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(21984..22424)
                     /locus_tag="ACG8E6_000091"
     CDS             complement(21984..22424)
                     /locus_tag="ACG8E6_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS5060552.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(22424..22999)
                     /locus_tag="ACG8E6_000092"
     CDS             complement(22424..22999)
                     /locus_tag="ACG8E6_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFS5060553.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            23239..23448
                     /locus_tag="ACG8E6_000093"
     CDS             23239..23448
                     /locus_tag="ACG8E6_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFS5060554.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            complement(23846..24358)
                     /locus_tag="ACG8E6_000094"
                     /pseudo
     CDS             complement(23846..24358)
                     /locus_tag="ACG8E6_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(24484..25185)
                     /locus_tag="ACG8E6_000095"
     CDS             complement(24484..25185)
                     /locus_tag="ACG8E6_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5060555.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(25293..26736)
                     /locus_tag="ACG8E6_000096"
                     /pseudo
     CDS             complement(25293..26736)
                     /locus_tag="ACG8E6_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(26750..28615)
                     /locus_tag="ACG8E6_000097"
     CDS             complement(26750..28615)
                     /locus_tag="ACG8E6_000097"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5060556.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHRGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(28736..29587)
                     /gene="licT"
                     /locus_tag="ACG8E6_000098"
     CDS             complement(28736..29587)
                     /gene="licT"
                     /locus_tag="ACG8E6_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS5060557.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(29897..30610)
                     /locus_tag="ACG8E6_000099"
     CDS             complement(29897..30610)
                     /locus_tag="ACG8E6_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS5060558.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(30678..31004)
                     /locus_tag="ACG8E6_000100"
     CDS             complement(30678..31004)
                     /locus_tag="ACG8E6_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS5060559.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(31127..31354)
                     /locus_tag="ACG8E6_000101"
     CDS             complement(31127..31354)
                     /locus_tag="ACG8E6_000101"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS5060560.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(31676..32929)
                     /locus_tag="ACG8E6_000102"
     CDS             complement(31676..32929)
                     /locus_tag="ACG8E6_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS5060561.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            32990..33730
                     /locus_tag="ACG8E6_000103"
     CDS             32990..33730
                     /locus_tag="ACG8E6_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS5060562.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(33816..34574)
                     /locus_tag="ACG8E6_000104"
     CDS             complement(33816..34574)
                     /locus_tag="ACG8E6_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS5060563.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(34571..35035)
                     /locus_tag="ACG8E6_000105"
                     /pseudo
     CDS             complement(34571..35035)
                     /locus_tag="ACG8E6_000105"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            complement(35054..35551)
                     /locus_tag="ACG8E6_000106"
     CDS             complement(35054..35551)
                     /locus_tag="ACG8E6_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFS5060564.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            complement(35619..38786)
                     /locus_tag="ACG8E6_000107"
     CDS             complement(35619..38786)
                     /locus_tag="ACG8E6_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFS5060565.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(39010..39894)
                     /locus_tag="ACG8E6_000108"
     CDS             complement(39010..39894)
                     /locus_tag="ACG8E6_000108"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5060566.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(39989..40426)
                     /locus_tag="ACG8E6_000109"
     CDS             complement(39989..40426)
                     /locus_tag="ACG8E6_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060567.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(40643..41554)
                     /locus_tag="ACG8E6_000110"
     CDS             complement(40643..41554)
                     /locus_tag="ACG8E6_000110"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5060568.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(42011..42439)
                     /locus_tag="ACG8E6_000111"
     CDS             complement(42011..42439)
                     /locus_tag="ACG8E6_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060569.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(42467..43105)
                     /locus_tag="ACG8E6_000112"
     CDS             complement(42467..43105)
                     /locus_tag="ACG8E6_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5060570.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(43109..45091)
                     /locus_tag="ACG8E6_000113"
     CDS             complement(43109..45091)
                     /locus_tag="ACG8E6_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFS5060571.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            45271..45936
                     /locus_tag="ACG8E6_000114"
     CDS             45271..45936
                     /locus_tag="ACG8E6_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5060572.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(47871..51020)
                     /locus_tag="ACG8E6_000115"
     CDS             complement(47871..51020)
                     /locus_tag="ACG8E6_000115"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFS5060573.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(51033..52232)
                     /locus_tag="ACG8E6_000116"
     CDS             complement(51033..52232)
                     /locus_tag="ACG8E6_000116"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS5060574.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(52222..53817)
                     /locus_tag="ACG8E6_000117"
     CDS             complement(52222..53817)
                     /locus_tag="ACG8E6_000117"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFS5060575.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(54326..55108)
                     /locus_tag="ACG8E6_000118"
                     /pseudo
     CDS             complement(54326..55108)
                     /locus_tag="ACG8E6_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(55173..56009)
                     /locus_tag="ACG8E6_000119"
     CDS             complement(55173..56009)
                     /locus_tag="ACG8E6_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5060576.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(56027..57436)
                     /locus_tag="ACG8E6_000120"
     CDS             complement(56027..57436)
                     /locus_tag="ACG8E6_000120"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5060577.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYRKVIARNGLA"
     gene            complement(57450..59273)
                     /locus_tag="ACG8E6_000121"
     CDS             complement(57450..59273)
                     /locus_tag="ACG8E6_000121"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5060578.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(59553..59780)
                     /locus_tag="ACG8E6_000122"
     CDS             complement(59553..59780)
                     /locus_tag="ACG8E6_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060579.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
     gene            complement(60116..60442)
                     /locus_tag="ACG8E6_000123"
     CDS             complement(60116..60442)
                     /locus_tag="ACG8E6_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060580.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(60488..60850)
                     /locus_tag="ACG8E6_000124"
     CDS             complement(60488..60850)
                     /locus_tag="ACG8E6_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFS5060581.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(60887..61102)
                     /locus_tag="ACG8E6_000125"
     CDS             complement(60887..61102)
                     /locus_tag="ACG8E6_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5060582.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            61229..61564
                     /locus_tag="ACG8E6_000126"
     CDS             61229..61564
                     /locus_tag="ACG8E6_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5060583.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(61640..63637)
                     /locus_tag="ACG8E6_000127"
     CDS             complement(61640..63637)
                     /locus_tag="ACG8E6_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060584.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(63735..64010)
                     /locus_tag="ACG8E6_000128"
     CDS             complement(63735..64010)
                     /locus_tag="ACG8E6_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5060585.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            64242..64976
                     /locus_tag="ACG8E6_000129"
     CDS             64242..64976
                     /locus_tag="ACG8E6_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5060586.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            65015..66157
                     /locus_tag="ACG8E6_000130"
     CDS             65015..66157
                     /locus_tag="ACG8E6_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5060587.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(66252..67643)
                     /gene="sufB"
                     /locus_tag="ACG8E6_000131"
     CDS             complement(66252..67643)
                     /gene="sufB"
                     /locus_tag="ACG8E6_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFS5060588.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(67648..68118)
                     /gene="sufU"
                     /locus_tag="ACG8E6_000132"
     CDS             complement(67648..68118)
                     /gene="sufU"
                     /locus_tag="ACG8E6_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFS5060589.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(68105..69340)
                     /locus_tag="ACG8E6_000133"
     CDS             complement(68105..69340)
                     /locus_tag="ACG8E6_000133"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFS5060590.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(69337..70623)
                     /gene="sufD"
                     /locus_tag="ACG8E6_000134"
     CDS             complement(69337..70623)
                     /gene="sufD"
                     /locus_tag="ACG8E6_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFS5060591.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(70636..71409)
                     /gene="sufC"
                     /locus_tag="ACG8E6_000135"
     CDS             complement(70636..71409)
                     /gene="sufC"
                     /locus_tag="ACG8E6_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFS5060592.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(71606..72454)
                     /locus_tag="ACG8E6_000136"
     CDS             complement(71606..72454)
                     /locus_tag="ACG8E6_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5060593.1"
                     /translation="MKKKIFGLAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(72478..73164)
                     /locus_tag="ACG8E6_000137"
     CDS             complement(72478..73164)
                     /locus_tag="ACG8E6_000137"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS5060594.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(73157..74191)
                     /locus_tag="ACG8E6_000138"
     CDS             complement(73157..74191)
                     /locus_tag="ACG8E6_000138"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS5060595.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(74254..74510)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(74586..74924)
                     /locus_tag="ACG8E6_000139"
     CDS             complement(74586..74924)
                     /locus_tag="ACG8E6_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFS5060596.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(74926..75282)
                     /locus_tag="ACG8E6_000140"
     CDS             complement(74926..75282)
                     /locus_tag="ACG8E6_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFS5060597.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(75288..76475)
                     /locus_tag="ACG8E6_000141"
     CDS             complement(75288..76475)
                     /locus_tag="ACG8E6_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS5060598.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYFYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVVGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(76666..77511)
                     /locus_tag="ACG8E6_000142"
     CDS             complement(76666..77511)
                     /locus_tag="ACG8E6_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5060599.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YPLPQLMKHLIKRFPKVHFEIEIANSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            77745..78710
                     /locus_tag="ACG8E6_000143"
     CDS             77745..78710
                     /locus_tag="ACG8E6_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFS5060600.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT        22962 a        16379 c        13224 g        26418 t
ORIGIN      
        1 ttattcttaa tataaatttt gattgtgaat ttaaaagaaa gaggtcattt atggaagata
       61 aaaaattgaa aaaacaactt agctccagac atatcaccat gttagcttta ggtggagcaa
      121 tcggtgcagg tctatttaaa ggaagtggcg aagcgattgg gatcgccggc ccttctgtat
      181 tgattgcttt tttgattggc gggctgattt tatatatcgt gatgaaaggt ctgggaaaaa
      241 tcgttttaag cggtggcgac acgcatcacg gattatctgg tttgatacgc ccttatctag
      301 gtgctcattc tgctgatttt actgattggg tctactggtc tatgtggatt atcaatatca
      361 tcgctgaagc tgttgcagct gcttcctttt tgcagttgtg gttcccgcac attccagcct
      421 gggtatttgt ttttatgttg gctgttttaa ctacgatcat caatctttat tcggtacggt
      481 tattcgcaga aacagaatat tggctggctt ttgctaaaat cagtgtcatt atcttactaa
      541 tcatttttgc tacttatctt gtcggtcagc aaatgttggg cacaggcgtt ttccctaccc
      601 ttcagcaatt gactgaccat ggtgggttta ctcctcatgg tatgaaggga atcatcagtt
      661 ctttgcttgt agttatctat tcttacggcg gttctgaact gattgctatc acggtaagcg
      721 aagcagatga tcctaaacgt gcgattccta aagccatcaa aggtgtcatg ggaagaatca
      781 tttcctttta catcattcct ttgtttttgc ttttgattct ttttccttgg aatactttag
      841 caggcacgaa tgtcagtcct tttgtgatgg tatttgaaaa aatgaatatc ccatttgcag
      901 cggatatcgt caattttgtt attgtccttg ctttgttctc atctatcaat tcgggtgtgt
      961 atgcatcttc aagaattttg ttcttccgtt tgaaggatcg aaaaggttcc tctagaaaac
     1021 tagctgtttt aaacaagcat caagtaccgc aacgtgctgt tttattttgt accagtgttc
     1081 tttatcttgg tgtagctctt tcttattttg tcggtgacaa attatttggc tatctggcag
     1141 gttccctttc gtatacagta ttattgatct ggatcattat cagtgcggca tcctttgtac
     1201 tggcactaag aaaaggaaca ttatgggata aaggaatcag cttttttgca ttagctgtct
     1261 taggattgat tttccttggt atcctgttca cgaactccat tggggtaact gtattgactg
     1321 gcctgcttta cctgttcatt tatttcagtt atcaaaaaaa gaatgatgcg ttcgtattga
     1381 cgaatgaatg aaatcgatag aaaattttat tttatttatg tggaatagat ggaactattg
     1441 aagaaaggag ttgtgacaaa agtcttgttg caactccttt ttccgaataa acagtctcat
     1501 gacctcttgg taaacggtgt tcaaaaaccc tctgtgcaaa ataattgatt gttctccatg
     1561 ctcatgacat gatagaagaa aggaatgaaa aaaaggagtt tgttttaaat gaaaaagtat
     1621 tggaagtttt tgttgatgat cggtgtttca acggtagtta tgtttggtat gatgtacttg
     1681 aatgtctatg cgctcgatca tatcttcttc agtcgtacac gagtattcat ggcgttgatg
     1741 atgggtgcag tcatggcagt catcatgctg ttgttcatgt ggaaaatgta tgaaaacaaa
     1801 aagataaatg ttatgatctt agccgctagt cttcttgttt ttgccggttc cttgtttatg
     1861 gtacgaagcc aaactactgt caacgacaca gcttggatga aggccatgat tcctcatcat
     1921 tctatcgcta tcctgacaag taaacgagct gatttaaaag atcctgaagt aaaaaaatta
     1981 gcggaagaaa tcatccaggc acaagaagaa gagatcgaca aaatgaaaaa aatgatcaaa
     2041 gaaatggaat gaccattatc cgtccaaata aaaaaagagg atgttccaga agtgatcagc
     2101 ttcaagaaat aagaagaagc tacttctgga acaccgtgaa taaggggttg tgaaaaagct
     2161 gccctgcatc aagtaataag aacagccaaa caaaaatagc ttctcctatt tttcgtttgg
     2221 ttaggcttat taccgcagat gcaagctttt gaacaccgtt tattcgtaaa agggattgtg
     2281 acaaaaaaag tcttgtcaca atcccttttt atgtttttat tgttctaatt gcgctgtctt
     2341 gcttactgct tcttgcggtt tcacaaattt ttttgagatt ccccaacctg tgaaggcact
     2401 gaccatcgaa aacacaggag aaagcaaact gaaaaatacg aatggcaagt aagtgagtgt
     2461 aggaatacca aatgtacttg ctacgaaact tccggcaatt ccccaaggca ctaagtagtt
     2521 gattactgtt ccaccatctt ctagcgttct acctaataca gttggatcaa atcctgcttt
     2581 ttcataagtt tctttaaatg cattaccagg cagaataaca gataggaatt gttcaccaat
     2641 aaaaatattt actccgatac ttgtcatcaa tgttgtaaaa atcaattttg gtgtgttcgt
     2701 catctttctt gatactcgtt ccatcacggt tgagatcaag ccaaattcga tcaacaatcc
     2761 acctaatgac aaagttagaa taatcaatga cacagtaggc atcatacttt cgattcctcc
     2821 gcgactaagc agtgaatcga tttgttggtt tccagtattt gaaacaaacc ctgattcgat
     2881 gaccccagcc aacttcgtta aggaagtttg tgggttttga ataaaaatca ttcctactgc
     2941 tacagcgata ttcaacaaaa tcgtcaaaat tgccggcacc tttttccatg cacacagtaa
     3001 cattaaaaat aaaggaatca gtgaccagat tgaaatagta aagtttgctt ctagaacatc
     3061 agtcacttca cgaatagatg aaagacttgc ggctttactt gtgtgcccta aaatcatgaa
     3121 taaaaagaaa gaaacaacaa atgccggaac agttgaccac atcatatgct tgatgtgttt
     3181 gaacaaatcg gcatcaacaa tagctgctgc tagatttgtt gattcggata atggagacat
     3241 cttatctcca aaaacggcac ctgaaacaat agctccaaca accagcgctg gatttaggcc
     3301 tagtgttgtc ccaataccga aaaatgcaat cccaatcgtt gacataacag taaaggcact
     3361 ccctacagcg ctacctacga ttgcacacac aacaaataca gaagggacaa accactggac
     3421 actgatcaat ttgaacccaa tgaccattag agttggaatg atccctgctt gtatccatac
     3481 agcaatcaat gaacctacta aaataaaaat aaagattggg atgattcctg gtttgatgcc
     3541 attgatgatt ccttcgtgca gccaatctgt cgggtatctt tttatcactg catacaacat
     3601 gaccattccg atcgctgcta agatcgtgat attaggtgag acctttaatc cgacaacgcc
     3661 aatcgcaatg cctaaaacaa taaccattaa tacaagtgtt gcttctaaaa aacttggtgt
     3721 tccttttttt cccatgataa tctctccttt tttagttgtt cttatgttct aaaaaagtta
     3781 agatatcgga ttactccgcc gcaattcatc cgctattcct cctttataaa aataaaaaga
     3841 cctgttttaa actaaggacg aatgactccg cggtaccacc ttaattaaaa acagatctgt
     3901 ttttcacttt caaactttta ttcaaatgta tggactttcc atgtaattcg tcaattaggc
     3961 ttgatcggtt cacagcgacc accgactttc tgaacaaaac acctaaccgc tactgcgtga
     4021 aaagttgact tgtatgaagt ttaccttcca taagatcatt tgtcaatgaa aaagaaaata
     4081 ttttttcatt tttctgaaaa ttatttttat aactttcaca cacaagttta ttttttacta
     4141 gaaagtagca ccctcttgtt tttttcttgc tactatttct tgatgttctt gcttgattct
     4201 ttctaatagc tttggctctt tcaacagatc aagagctgta ttggcgagta gttctgctgc
     4261 aatcgcaata gaagcaaggc ctttgtcact tttagcagca gctttgaatt cttctgtgtg
     4321 tccggcaata tatgtctcag agattgctac cgtaggctgg attgttggta tgacttggct
     4381 gacatttcct acatcagaag aaccgagact tgttctttgt tccgtttcta tttcttcatc
     4441 tggaacacct gcttcttttg catgctggaa gaataagtca tcaaaagaag gagtaacgat
     4501 gacatcatca actgcatttt ggaacaatcc aaactcaaaa gtcgtgcccg tacttatcgc
     4561 agcacctcgt acgatatttt ccactttttc atatacttta tctaatgttt gccgatttcc
     4621 tgcacgtaaa tagaaacgtc cagcagcgta atctggaatg acgttggcag ctactcctcc
     4681 gtctgtaatg atcccatgga tattgacatc ttttggcaat tgcaagcgta gagcgttgat
     4741 tccattaaac acttggatca atgcttccaa agcatttact cctttttcag gtgctgctgc
     4801 tgcatgggaa gcgacaccgt aaaatttgat gtcgactgga tcattcgcta aagattctgt
     4861 cgttttccca taacgaaaag ctggatggac gcataacgct acatcgacat cctcaaaaaa
     4921 accttctcga acaaagctgc ctttagctga tccgttctcg cccccttctt cccctggggt
     4981 gccatataca cggatttctc caccagtttc atcaataaat tgctccacta tgctggcggc
     5041 taatacggag taagtaccaa acaaattatg cccgcatgca tgaccaatac ctggtaaagc
     5101 atcgaattct gccaaaaagg caattgtggg tccgggttta ttggaaatat aacgtgcatc
     5161 aaaggctgtt cggtgtccag ctacttcttt ttctacccaa aacccttctt tttctaattg
     5221 tttaatcaac gctgctgagg aataaaactc atagttactt acttcaggat gttcgtggat
     5281 atctagagca agtgcttgat attctggtaa acgatattgg attagatcct tcaattggtt
     5341 tgttgagatt gttgttgtca taaaaaattc ctcctatgct agtcattttt cttattttct
     5401 cccgaatgtt tcccatgcag gcacgcttga tccttttgat gttttctcga ttactttgac
     5461 tgtgtcttct gtttgataag cgtctactat tttttgatac gttttatttt tttcatcttc
     5521 ttttcttgca acgatgatat tcacataagg acgtgcatgg tcatccactg gttctaagaa
     5581 gatggcatca gaagcaggat taaatccagc atctacagct acaccgctat tgatcactga
     5641 aatgtctaca tctgacaaag cacgtgctgt ttgggaagca tctaattcag aaatgtctag
     5701 attttttcta ttctcggtaa tatcagaaac agtgggcgct tgtccttttg ccttatccac
     5761 tttgatcaat ccagcagttt gcaataacag aagagctctg ccgccatttg taacatcatt
     5821 agggattgct acttttgctc cgtctttgat atcagctaca tccttcaaag tgttagaata
     5881 aattcccagc ggtgcattga cagtatttcc gatcgatact aaatcagttc cgtgttcttt
     5941 attgtaattg tctaaaaaga attgatgttg gaaagaattc aaatcaatcg atccatctgc
     6001 taaagccgca tttggttgat tgtaatctgt aaatttcacg tattccaaat taatgttctc
     6061 ctttttcaaa cggtctttca cgttgtccca tacatctgta tcttctccaa taattcctag
     6121 ttttacggta gtttcttttt tgtcagttgt atctgctttt ccttggctac accctgccaa
     6181 ccaaataatt gctacaaatg taatgactac aaaacgaata atttttttca ttttttctcc
     6241 tccactttca atgtgtaatt tttttaatca gttgattact gataaattga cttaaaaaaa
     6301 ctaagattag aatcaataat gtagccacaa tcgttacatc tgtttggaat cgattatacc
     6361 ctctcgatat agcaagattc cctaatccgc ctgcacctac tgctccagcc atggtagtca
     6421 gtccgatcac gttgatgata gtcaacgccg agacccgaat gataccaggc aaaccttcta
     6481 acaaatacac acgtaaaata ataccgattg gactggttcc catcgcttct gccgcttcga
     6541 tcaccccagg atcaacttct agcagtgcgt tctcgatttg acgagcaaaa aacggaacaa
     6601 ctccaaaaat caatggcact aaagctgctt tcgcaccaat cgtcgttcca actaagaaac
     6661 gagtagtcaa tgctaaaaga gaaagcaaga taatgaaagg gatggaacga atgatattga
     6721 tcagcttatc aagtatttca aatagcagac ggttagctag taatccatta gggcgtgtca
     6781 ctactaacgt cacacctaag atgattccga tcatcccgcc aatgaatgct gtccaaaaag
     6841 acatatatag tgtttgcttc gtcgcttcga caaattcatc cggaatcaaa taaacattgg
     6901 gcaaatatgt tttaaagaaa tcagtcattt tatcttcctc cttcgatcac tttgatacga
     6961 ctatctaatt cgtttttttg taaaatattt atttttacac cagatttgat caaataatcc
     7021 aatgcttttt gccgctgttt ggtttctcca gctaaagtaa cgatcaaact ccctaacggc
     7081 gtttcttgta ataactctac attgccatat aaaatattcg ctgttacttg atatttgctg
     7141 tacagatgag cgattagagg ttcgttcgtc tgatttccaa tataacttaa ttccaccagc
     7201 catttattgg cgatctgatc ggcaaaagca gaatgggcaa tgatcgtttc taatgcttgg
     7261 tcaagatgag tagctgttcg aataaaatct ttggtcaatt cttcttcagg attgctgaag
     7321 atctggatgc tactaccttt ttcaatgatt cttccatctt ccataaccgc taccttgttg
     7381 cagatttctt tgacaacttg catctcatga gtgatcagga cgattgttaa acctaactgg
     7441 cgattcaatt tttttaacaa tgcaaggatc tgaagggtcg ttttcggatc aagtgcactg
     7501 gttgcttcat cacataataa taccttaggt tcattcgcca aagctcgtgc gattgctact
     7561 cgttgttttt gtccccccga cagttgttta ggataagcag atgctttttc ttccaaaccg
     7621 acaagttcca gtagttcatt cactttttga cgacgttcct gtttcgattt tccagaatac
     7681 tttaaagaaa aatcaacgtt gtcaaaaata gttcggcttt ccattagatt gaaatgctga
     7741 aagatcatac cgatggcctt tctttctttt cgcaattctt tgggagagag ctgactcaaa
     7801 ttcgtctgat tgacaatgac ttctcctgct gttggtttct gcagcaaatt catcacacga
     7861 actaacgtac ttttccctgc acctgagtaa ccgacgattc cataaacatc gcctttttca
     7921 atcgttaaat ctacagaatc tactgcctgt atctcttttt ctttttgttt gaaacataca
     7981 gaaacatctc gcaattcaat cattaaaatt cctccttatc taaaaaaagc gtcctcaaga
     8041 aaatgaaaat gtcattttct tgaggacgct tttttgcgtg ttaccacctc atgttcaagc
     8101 tgtgttcaca cacaactcct cttcaagtac gatcatactc tagcaagata tcggtcgcag
     8161 ccgtcatcgc cttacatcta tgctcgggat gaattgctcg atggccattt tccaatagtc
     8221 gttccttgct tcttctcagc taccgaagct ctctgtcaaa ggttcttcta tcgtacttac
     8281 catttcaatg caaataatat tcaaaaaaaa atccttttac taacataaac tatgtcagta
     8341 aaaggacgac gtatcgcggt accacctttt ttcggaagtt tccttccctc aagcccaaca
     8401 tctcaaaaag agagattggc gttcgtttta acggtcgaac agttccgaat ttgcctactt
     8461 atcgacaaat tattctccaa ggccattttc agcttttctt acttgactct tctcaccagc
     8521 cagagcctct ctgcaaagtt tccaaactta ctttcctttt caacgaaatc gatattttgt
     8581 tgttgggatt aatctacatg aggaaaacta aaaagtcaag cacgaaattc aaattatttt
     8641 ttctatattt atttttctgt ttttttatct aacatttcaa taacagttcg aagatctttt
     8701 ccttcagcaa gaagtttcaa tttttccttt tcttcgacgg tataatttcg gacaaaactg
     8761 gaatcaggta tcaaaacaac gcctaatcct gctcgtgaag cagctgttgc tcctactaaa
     8821 gaatcttcca ctgcaaaggc tgaactagct tctaatcctg ttcgttccaa agctgtcaga
     8881 taaggaatcg gtgaaggctt gttctccgac acttcgtctc cgaatgtaaa cgtgtcaaag
     8941 taatcagcta atttaaagtg atccaagata tctgttgctc ttttcgtaac agtggtcgta
     9001 gcaatgccta gtttcatatg acgttctttt aagtctgcca atatttcttt tgcatatggt
     9061 tttaagtgga tggctccgct tgtcagctgt tgataaaaat attcttcacg cttcgctcgg
     9121 acttcctgta ctttttcagg gctacctgct gctttcacta aaatatcaaa actttgtttc
     9181 cagttttttc cgccccaagc gtcaacgatt tcttttggga tatggatatc aaattgctga
     9241 aatccccaaa gccagccatc acgatacgtt ttttctgtgt caactaacaa accatctaaa
     9301 tcaaatattg cacctttcat ctatacatta atcctttcca aaacaataca catagtttag
     9361 cacgactggt ccaaaaataa cagaagcaaa gaaccagcct tctcgtttat aagagttctt
     9421 ggaatcgatg gatatagatt ttcttcacag ctgtcacaag cgccagataa gcgatgactg
     9481 tagcaaataa ccataaccag taattgccag gaagtggcat caagcctaat tcagcaccaa
     9541 aacttgtaaa aggaaggaca gaaccgacaa taattcctaa tgtagtgata acaaacatgg
     9601 caaatgttac gttgctttgg ataaatggta ctttcggtgt acgcagtgca tggagcacta
     9661 aggtttgtgt ccatagtgat tcgacaaacc atcctgcatg gaataatgcg atgaaaatca
     9721 cttgctgtga actatccaat gtatggaaac ttccgccaac tacagcagga caaataacaa
     9781 aatacatcaa caagtaagta gtaatatcaa atatcgaact tgttgggcca aagtaaacca
     9841 tgaatttccc aatactagat gcattccatt tttttggctc gtccaaatat tctttatcca
     9901 tccgatccca tggcaaggaa atgcaggaaa catcatagat tagattcaaa aacagaagtt
     9961 gtaaaggcag cattggtaaa aatggcagga acatacttgc aatcagcacg gaaaacatat
    10021 tgccaaaatt agaacttgct gtagccttga tatatttcat gatattccca aaagtttctc
    10081 gtccagaaag aaggcctctt tccaaaatcg tcaaatcttt ttctaaaagg atcacgtctg
    10141 cagattcttt cgcaatatcc acagctgtat ctacagaaat accgatatca gccactttca
    10201 ttgcaggagc gtcattgata ccatctccta aaaatccgac agtatgacca tttttacgta
    10261 aagctgtcgt caaacgtgct ttttgctgag gattcaattt tacgaatact tcatggtctt
    10321 cagctacttg tgctaattca tgatcactca tttccgagat tttctcaccg gtaaccaatt
    10381 cattgatctc tagacctact tgacggcaca ctgaacgtgt aactaattcg ttgtccccag
    10441 tcagaacttt tacagctaca ccatgttctt tcagagcttt caacgcttgt ttcgttgttt
    10501 cttttggtgg atcaaggaaa gtaagataac caatcaaaac catgtctgac tcatctttga
    10561 ctgaaaattc tccaacgacg cttggattgg tcttttgtgc gacaccaatc actcgtaagc
    10621 catcttcatt aagtgactgt acttttgcaa gtacggcttc ttttcgttct tttgttaatg
    10681 gtgtaacagt accgccaaga tcaataaatt tagaaatgtt cagcatttct tcaatcgcgc
    10741 cttttgtgat catttgtgtt ttaccagctg gatcagcaac cacaacactc atacgacgac
    10801 gttcaaaatc aaaaggaatt tcatccactt tttgataatt gatcttatct gtatctaaag
    10861 tttctgttgc ttcatcaatg atagccacat ctaacaaatt tttcagccct gtttgatagt
    10921 agctatttag atacgcatgg cgcaaaacac gatcatcttc atttccttca caatccatat
    10981 gatattcaag aatgatttta tcttgtgtca atgtccccgt tttatccgtg cataacacat
    11041 cgattgcacc aaagttttgg atggcattca aatttttgat cactgttcct tttttggcca
    11101 tcacacttgc tcctttgacc aaattcgtcg taacgatcat tggaagcatt tcaggtgtta
    11161 gaccaactgc tacagatagt ccaaaaagaa aagcttctag ccaatcccct tttgtaagcc
    11221 cattgatgac gataactgct ggagccatca atgacataaa tttgatcagt agcatggatg
    11281 ttttatggat gcctaattca aagcttgatt gaattggttt ttcagatagc gtttctgcta
    11341 catgaccaaa tagtgtttca tttccaacag aaacaatgat tccttccgca cttccactta
    11401 tgacattact tcccataaag acaagatttt cgtaacttgt ttcgctttgc tgatcgtctt
    11461 cacatacttt cgcatgcttt tctactggat agctttcccc tgtcaatgct gcttgggaaa
    11521 tgaataaatc ctttgcggac aataaacgca tatccgctgg tatcatatcc cctgctgaaa
    11581 gccgaacaag atctccacag acgatttctt ccataggaat ctctgtgtac tttccattac
    11641 gttttacagc tgccgttact ttcacaagtg atttcagttt ttcagccgct tgattggaac
    11701 gtacggactg aatcaacgtc attgtcccgc tgacgatgac catagtgaag ataatggcta
    11761 caccgaaaag atctttatct ccaggagctg ctaatacata ttccgtgatg aatgaaataa
    11821 ttcctaatgc gatcaacact aatgtaaatg gcgtgatgta tgctttcaat atttctacga
    11881 tgaatggtgt ttttttgcca tatgaaatcg cattttcacc gtaattttct cttgaacgtt
    11941 cgatttgttc ttctgtcaat ccatttgctg atgcgtggaa tagttcaaat aattcttgtt
    12001 tcgtttttcc agcaaaagct gcatagattt tttgtgtatc tttctttttt tccatctcaa
    12061 tctctccttt gtgtccaaaa gacagctgtt ctcgctgctt ttggtcttta gctattcatc
    12121 ggatcgatag agaaaaaact gaaaggggct gagaacagat agataatagc cttggccttt
    12181 cctctagatc cttgtttttc gaatgactgt catccatttg ttctcatctc ctttactaag
    12241 taattttaat tcatgacttc caatcatttt tcgaatagtt attttcccaa ttagaccacc
    12301 tcctttttat ttttcaccga tggggatttg cggcaacaaa aaaacctctc gccaaatact
    12361 cagcgaaagg tttgaaaaag catattaaaa aaagcttctc agatctcatc gttgagtttt
    12421 gacactgtac aacgtaaaaa gcagatacct gcttttagct accttatgaa aagccttatc
    12481 tcgacaattc ctgttctacc cattggcgtc tctcgacgtt tttgggcagt agcctatgtt
    12541 tgtataggag cctcacctaa caaatcttat tcggttttcc ttcgttaata taacaactaa
    12601 aaaagaggat gtcaaatcaa ttttccatgt ttttcatact ttttttaaaa tacacacgtt
    12661 atattcctgt ctcacgcggt tttatttgtc tcattttcca cctcccgcta taatagaaat
    12721 atagtaggaa acgaggtgct gttatggaaa gaacacttgt tattatcaag ccagacggcg
    12781 tgagacgcca tttagtcgga agcatcattc aacgttttga agcaaaggga ctggccattg
    12841 ctgaaatgaa gtttgaaact atgactcctg aattagcaaa agaacattat cagcacttga
    12901 ctgagcgcag tttttttgat gaactgatcg attacatgac ttccggtcct gtcgtttatc
    12961 ttgtgctggt cggcgaggaa gtgatcgata tcgtccgtaa gatggttggg gcaacaaaag
    13021 ctgtcgatgc tgttccaggt acgatccgag gagactacgc gctgccgggg actgaaaaca
    13081 tcattcacgc ttctgattcc cgtgatgctg cggtaaaaga gatcgcccgc tttttcccgc
    13141 cattcgatat aaacaaaccg tctgaaaaag cggcgaagtg atacaaattc taaactgctg
    13201 gtctagttgc tgggttcagc aatggccagc agttttttct ctaaggacct ccgttgattc
    13261 tttcctaaca tatattcgag cactgtgaag aattcttggt agtctttcgt ctttttttcc
    13321 cctaaagcag cagaaatcaa tctttgataa taatctgcca tcaatgtttc atagatcact
    13381 gtacttcttt ctttatgtgc taagctccgt ttcatatctt cattcacact acgtgccttt
    13441 ttcaaccgac cattttctat ataaaacgaa gtcaggttca ttaaaaaagt aaagagatac
    13501 tgctcatgat tgttcaactg tgctaatcgt cgcattcttt ttttgatttt caaataattg
    13561 ttctcaatga accgatcact gaatatccaa ggaaaatcag ctagaataga tagttcaaat
    13621 tgaccatagt ctggtatctg catcaagtat tcttcaatga ttcggcattc ataacgtaaa
    13681 aattcaaatt ctgtctcaga tacttctgtt ttttcgacca tgtgaagact caaaaccaaa
    13741 taatacagat cttctgtttt cttaaaatgc ctacggttat tgctgatctc tttataagga
    13801 tcaaagctgt cttcttgtac ccgatctagc attcgtcttt ttttctttaa ctttattggt
    13861 tcatccagct ggtttcgcat ccattcatat tctgcaagtg tcatgttcat tcgctctaaa
    13921 aatgatctca atgttacaaa tgacattttt ttcactgaat ttgtctcaat cccgactaga
    13981 gaagtcttag aacagattcc ttccgctaat tgggactgtg tcaacttctt gttgactcga
    14041 agtttcttga tcaattcacc atcatacata atagttctcc tttttaattt aaatttttcc
    14101 acattcaata aatagatacg ttaaaaagga aaaatacacg aaaaaaataa aaaaatgttt
    14161 ttttcataaa aaataccaac cttcaagcat tagaatgttg tctaactact gaaggttggt
    14221 ttgattgttt attgattatc tttttgctgt tcaacgtctg ttcttgttac tggatataag
    14281 ttggttgttg ttttattgcc attttttgaa aagacacttg tcgaatgttc gcctttttgc
    14341 ttttcgatgg ctatgttacg ttctgcttca tttttatagt catatttttt cggatcgatc
    14401 gtttcaaatc cgtttggcac ataaaaacgc aagagatttt cttgattcag cttatctgaa
    14461 agacttaaag cagatttgac tttttcctga tctttggaaa tttcctcgtc gattcctgct
    14521 gctttcgtat cgatgatttc tcctgtttgg ttattgtaag cttttcctcc taagatggtg
    14581 tatttgggtg tcacgaagtt cccatttcga aaagccacta cttgatcatg ctgaggagat
    14641 aataaatccg taccaaaatg gagatagtct ttatcatcga ttcccaagag atgaagtaat
    14701 gtcggcaaaa catcgatttc tccaccgtat tcgtgattga ctgacccttt agtataacca
    14761 ggtatatgaa tcattaatgg cacacgctgc atttgtgcgt tgtcaaattc attccatgta
    14821 tctgggtctt ttccaagagc acttgctaag tctttattat ctgtatttga gatgccaaaa
    14881 tgatccccat aaatcacaaa gatcgaattt tggtaaatag cggatttttt caggtacgta
    14941 aaaaattgtt ccaatgactg atccaaataa tgtgccgttc gaacatagtt atctactgtg
    15001 ctatttccag tgttcaaaga tggaaaatcg aaattcttat catccgtata ataaggcgtg
    15061 tgattggtta cggaaaggaa tttcgtgtag aacggctgtt gtagatgttc aagatattgg
    15121 gcagattcac tgaataaatc tttatctaat attccataac ctagcgtttc atctgattca
    15181 tcaaaataag atcgatcaaa gaaattgtca taacctaagt ttttatatac atgatctcta
    15241 ttccaaaaac tagctacatt cccatggaaa acagcgcttg tatagccttg cttttgtcca
    15301 aggatcgctg gtgcggcttg aaagacatta tctgacccaa gttctgtgaa taatgagcct
    15361 tgaggtaaac caaatgtgcc agtttccaac atattttcag catcacttgt ctttccctgt
    15421 cctacttcat gaaaaaaatt atcaaagctg atcgttgctt gattctggaa aatactattt
    15481 aaaaacggtg tgacttcttg tccgtctacc ttcatattaa tcaaaaactg ctggaaactt
    15541 tctaaatgga gaacgatcac atttttccct ttagcgatcc caaatgtttc gggatttggc
    15601 tctgcataat gtttctttgt ataatctaaa acttcatcaa tcccattact gcttgcatgc
    15661 gcgcgaacac tgctagtcat tcctgttttg attccatcat aaatagtata cgcatcgatt
    15721 cctagatatt tgacaatata tgaacggtca aatgttcgct gcagcaattg tggacggttg
    15781 gcttcgctca gcgctagatt tcctgaaaaa ataagccctg aaagacaggt cacagcgata
    15841 gccatcggtt tgctgacagg ttgacttttg ataggtattt tcttgatcgt caaccaaacc
    15901 agtatcccaa tgaacgtagc aatatccagc cagtagagga catcatgggg tttcatcaaa
    15961 gaaaaggagc ttcctgataa cccttgcgac actttcgaaa atcccaatac agatttgact
    16021 gtaatgaaat ctgtgaattc ccgataaaaa ataatgttca agtacaacaa aagcgtgttt
    16081 aaaatatcaa tgatcaaaag aatggctaat gctggctttg gctttttaat atacaaagaa
    16141 aggccaaaaa acaataaggt agttgcaatc gggttgatcc acaagataaa gtactggatc
    16201 gtaccttcca cacctaacga aaaatctgca tagtaagcaa taatcgtctt catccagaaa
    16261 aagaaaacag ctgtcaaaac gattgctaaa cgattttggc acaatttttt aagttgtgtc
    16321 acgtatatca cttctctcta ttcacatttt tccttatttt gctcctgaaa aatgaaaatt
    16381 ccctaaaaaa gttgttatct taagattttt ttattctttc taaagattat tccgtgcact
    16441 tttcacagca taaaaccgct gtgatataag cgattgaaac agtaaggtga taaaagtctt
    16501 gtcgcagctc ctgtttagtt gatgcaggac agctttttca caacttcttc tttaaacggt
    16561 gttctcctgc caactcctcg gccacaagtc acaatattta taaaacatga gaagctgttt
    16621 tttgaaattg ttccttgtgg ctcagagtta aacggcaagc tcaagacctc ttgatacacg
    16681 gtgttcaatc agctgacctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
    16741 ttgccgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct ctgtttttca
    16801 ttgtccatag gattgatatt gtccttgtga tgactcattc gtaataattg aggcttgatt
    16861 ttcttcactt cccgttatcg tttgtccact gccagaatct gaaggacttg ttcctgtcga
    16921 ctcgtttttt tgagaagaga cagttgtttt cttgacttgt tcatttgtct ttttccattc
    16981 cgctaaacga tcggcgacca cttttttgtc tactttttca gaataagcgg tgatcacttt
    17041 tccatttttc aaacgggcta ctttgtcgcc atcatgagtc aaaagcaaaa cggtcaaatg
    17101 cttgtcttta aattgatatt gtctgagaga aatcgcacta agtacgtcat cgatattaat
    17161 aaattgattt tcttcttttc cttttggcaa atagatgtcc aattcgcctt tttcgtaatt
    17221 ggcagttatt ttatactccg cccaccaata agttgagaat tcttttttga aattatctac
    17281 tgattctttg cttgctacga ttttttctgt ttctttctga gcaagatctg ctgtcatttt
    17341 tcctgaaaat gctgcggata aaataagcag taagaaaaaa ccgaataacc aaaagagaag
    17401 aaacggtctt tttttcctca tgctttcctc cactcctatt taattttgac tattctatta
    17461 tatacaaaaa atgaaataga aagacaaaaa agttgaaaaa gatcgtaaat aattgaactt
    17521 ctttttcaca aaatggtaag aatggagaat ttcacgcatt tgctctcaac tattctttct
    17581 taagcctcaa atccgttttt atctgatacg tctttgaacc agtgacccga ttttttgatt
    17641 gttctttttc catcttgtgc taagtctact gaaataaagc catatcgatt cttatatgca
    17701 ttggtccatg accagttatc catacatgtc cacatatgat agcctaaaca attggcacct
    17761 tcttgaattg cttgatggac atatttcaga tgagattgga caaatttgat ccgataatca
    17821 tcgtggatca tgccatcttc tttgataaat cgtgcttcgt tttctacacc cataccgttt
    17881 tcagaaataa agcagtcgat atttccataa tgatctcgta cattgatcaa aatatcatag
    17941 attccttgtt catagatctc ccatccgcga taaggattca ttttacgacc aggcatttca
    18001 taattgtcga aataatcctc aggcattgga ccgtttttag tatcgattgg tgtttctttt
    18061 gcttttaccc ggcgtggctg gtaataattg atacctaaaa gatctactgt attttctttg
    18121 atgaccgtca aatcctctgg atggatcaca ggcatgagat caagttcttt tactatctca
    18181 atcaattcac ttggaaactc gccttttaca gctggatcaa ggaatgaacg attgaagaac
    18241 gcatctgcta tttgcgctgc tttaacatct tcagggtttg attcgtctct tggatagctt
    18301 ggtgtcagat tcaagatgat accaataaca ccaccttgct ccatttcatg gtaagatttg
    18361 atggctaacg ctgaggccaa cgcttcatga tagccaactt ggatcgcttt tttcatatct
    18421 gactcttctg gataatgcca tcgatataaa taacctgctt cgactggaac gattggttca
    18481 ttatgagtaa accatttttt tactttcgaa ccgaacaatt caaaacaact tttggcatat
    18541 ttttcatagg cttcaaccgt ttctcggttc aaccatccgc ctttttcttg cagatacatc
    18601 ggcatatcga aatgatacag attcacaaag ggttcgatac cgttttcaat caactcatca
    18661 aaataacgat gatagaaacg aacggcttct tcgttgattg ttccagattc atcaggtatc
    18721 aagcggctcc attggataga tgttcgaaat gaattatgcc cagtctgttt catcaactgt
    18781 acatcctctt gatacttcgt ataaacttgt gaagtttttt caggtcctac ttggtcaaag
    18841 aacttttgcg gttctttttt gtaccaataa tcaaaaatgt tctctccttt tccatcacct
    18901 gaaatccttc cttctgtttg tgggccactt gctgcagaac cccaccaaaa atttgctgga
    18961 aattgatatt ttttcatcaa agcacctctt tttctttttt agttcgtttt agttgattcc
    19021 atgtatgata ttttccatca caattacttt ttcattttct tcctcttctt ttttgattcg
    19081 tttcaacagc tgttctgcgg cttgttcccc gattttatct gttggtggtg taatcgtgat
    19141 tttcggaagg atgctcaaat cgtttttatc caaataatca taatgactaa acgcaatatc
    19201 cttcccgatt ctgatacctt cttgattgaa acattcgata gcaccgatcg acatattata
    19261 attgctgaca aaaatcgctg ttactccttt ttctaataat tcttttgccc cataatagcc
    19321 actttttcta gaatagtctc ctgtaaagac ctctaattgg atcgatggat cattttgtac
    19381 cgcttctttt gctcctgcta aacgttctct tgctacgtag tctgtttgag tggcagcaat
    19441 aaatccgatt tttttatgtc cttgttcacc atgatgagcg atcacttttg ctacactttc
    19501 tcgatcattc actaatattg tatcaatacc tgctggtcct tctgggcaat tcaatgcgat
    19561 aactggaata ctcatttttg tgagcatttc aaatccagac cactcttctt cggataagaa
    19621 gacaatcagc ccatcgatca catgcttcat caggtattca attttttgtt caattttttc
    19681 tggctgatcg gcaaacccgc ttaataataa gctataccca ttttcttcag ctactctttc
    19741 gatccctgag acaacttccg acccaaaacg gctggaaatc ccgtttatca aaagtccaat
    19801 agagaagctt cgattattct tcaacccttt agcaatgatg ttttctttat agtccaacgc
    19861 tgaaatcgct tctgctattt ttttcatgtt cttttccttc agttgctgac cattcaagta
    19921 acgggaaact gttcccactg aaacaccagc catcttagcc acgtcacgga tcgatgccat
    19981 acattctcac tcctgccagt tacatttgat ttctctttct tgttgttcga ttatgatgac
    20041 cgctttcttt gcatagtgtt gcttgtcatc aatatccata taactaaagt cttcagtaga
    20101 gcaacttcct ctcactatta attatactta aacaagtatt tgaaccggtt catttttaat
    20161 atagcatttt tttaaaagat tgcaacactt tttttaaacg ctttcctaaa atggcgaatt
    20221 ttttgccttt ttaaaatttt attctaaaat ttactctatt tgtgggtgtt ttgtgcgttt
    20281 ttctcttcca tccgtttgaa gttttacttt ccaatggtat aatttcttta tcctatcaaa
    20341 tgggggaagt attttgaaga aattaatcgt actatttatt ttgtctgcgg gacttttgac
    20401 attgactggc tgtaatttgt ttggcggctc tgaccaatcc tcggatgaaa caaccgattc
    20461 ttctgtctta aaagcggaaa aagcagatat cactaacagt gtatatacgc taaaagtacg
    20521 taaagatatg gatttatccg cgggacagtt gacatttaca gataaaaaaa tggaatggac
    20581 acgcacgtat cagacacaag aaactgattc ttctgaatca tctgaagaaa aaacagtgct
    20641 gacagacatc aagattacta ccaaagatga cgaatatatt atttcaggaa aagaaaataa
    20701 aaaagaagca accatcacat ttaccaaaat cggtaattac cgtttgaagg atgaggatgg
    20761 caacatctac agtctatagt gccgtctgtt ggatcttctt ttgtttctcc ccttcttttc
    20821 tgctatagta aagaagcaag atgattgtaa aggagacgaa atggatgatc gatagtcctg
    20881 attattgtac atgtaataac aacaaaaata tccatacaga atttaatgac caaggaagct
    20941 tgagcatatg cgacaactgt cacaaactag tcaaagattc atttaaaaca tttgaatctt
    21001 atcctgttca aaattatgta aaaagtagaa gaagataaac aaatccaagc aaaactcact
    21061 atggctgatt tttatgaact tcaaactcag cttcttcctt tgatcaagct tcatcagtaa
    21121 aacaaacagt ggatcatttc agtttacaaa tcaaagagta aagatttatt tatttcaaat
    21181 atttgaatat aaaataacca aacgatagaa aggcttattc tacatcacgt agtaattttt
    21241 ctttcccgtc gtttggtttt ttatttttag cctaattctt gaccttttgc atgctggtta
    21301 tccgtaaaat attttccagc aggctttcct tccaattcag ctaatttttc ttggatctga
    21361 cggatcacct tttttgcatc ttctggattt tctacaaaat cgaatttatc cccatccact
    21421 tgtattttag gacatacatc aaaatcttca taccattgat tatagcgggt gttcaacgtt
    21481 tcataatagg tatataacgt ctcatcgctt tctagctgtt catagtcacg cccacgtttt
    21541 ttgatacgtt ctagcatcgt gtccaaagaa actttgatat ggaccatcaa atcaggccgt
    21601 ttttttggag caacatcatc taactcgttt agcatcgtat ctaataagct atcgtactgc
    21661 tgtacttcga tatccgttac ccgacctaaa tcagcgttta gatggaacaa caggctatct
    21721 tcatagatgg atcggtctag cacattgtca tcgttcacta aagcattttt cattgctaaa
    21781 aatcgcttgt tcaaaaagaa gatttgcaat aaaaaagcgt actgctcagg atttgaataa
    21841 aagagcggca atacttcgtt atcttcgaca ttttcataaa atggtttaga atcaatctct
    21901 tttgcaagca tatccgtcaa actgctctta cctgcaccaa tcgttcctga aatcaaaatc
    21961 actgacatac aacccttcct cttctataag tactcgccta cgtaagtttc aacaggcaat
    22021 gtttcaaaat catattcacg tacagcttgg tctgatttta aatacgtatg gagactttgt
    22081 gtaatcatca agttcgtagg taccgtctct tcttgtacaa tgatcatcgg ttttttcaac
    22141 atagttgcca cacctaattc gtaagccgtt cctggatcga ttgtttgatg ctcaaagtca
    22201 aggatagcaa caacaaattc tgcatttgtc agttcttcca tatctttttc gtatacttcc
    22261 tgcgcccatc cagcgctgaa tagttcataa ttgctttctt ggtgatgacg tggagagtag
    22321 aaacttgtga ctgttttgtt ttcctccaac gctttttcga tacgacttac tcgatcgatt
    22381 tgctcctctg aaaaaaatgg tcctgctaaa tatatttgac tcattatttt gcctccttca
    22441 ataataactg atttttaata gatactgctc gtaattcatt gaccagttcc aatggattgt
    22501 tggaaaagat tacttgatcc aaccattttt tatccataaa atcctctttt gcaaaagttt
    22561 ctaataattg ttgcaagccg ctgtaataac cgccaatatt caatattccg attggcttgt
    22621 cgggcgtcag accgattgct atccaagaaa gaagttgtga gaattcctcc aatgtaccta
    22681 gacctccagg gaaaacgagg acagcatctc ccttttcaaa taataatacc tttctttcat
    22741 cgatcgtttc tgtaggaata aatgtagtaa catcttttcc aggcaattca gtttcaaaaa
    22801 gcccattcgg atagatacct gtgacttttc cgttattttc cagtactgtt ttactaatca
    22861 ctcccataat cccggagtat gaaccaccgt aaaccagttc gatttcttct tgtgccatgt
    22921 attttcctaa cgtttgcgca ataaatttgt aactttcttt attaccaaaa cgggaaccgc
    22981 aaaaaacagt aagtttcata aaaaaacact ccctaactca aaagtctatc ttcattctac
    23041 acgtgttttt ttaaaataaa agactagatt ttgtagtttt ttagtggtat ataggataga
    23101 caccactata tatagtattt agaaaaaaac atttttcagc tattttatag ataaaacaaa
    23161 aataaataac gtgttttcat cgatgtgaaa tgacttactt atagaagata ctgggatttc
    23221 gtcctatatg ccaataacgt gtattatttt aaacaacaaa tacttgtttc ttttattaac
    23281 gaaaaaatca ttttgttctc gtgttatgtt tcactgataa atgatgtttc tctaaataaa
    23341 aaagccgacg aactaaacga tcaaaatttg atcatttttg ttcatcagct tctcctaaaa
    23401 atctttttgt taacgataaa tagctggatt tgtaaaaata tcacttgaat cgtcactgct
    23461 ggaagaaaat tcagtgataa ccgcacctgt tgcgcctgcc tggaactagt gtttgatatt
    23521 gggctcgaac gtgtactgat ctctagcatg aagaataatt tcttttttta ctgtataata
    23581 actttctttc ctttttggaa tagtacagga aagttttttt gtttcctttc cttcgacata
    23641 aggtatacca gaccctttcg acaacgaaag gttctcattt ttccttcatc gtcatttcaa
    23701 ggagggtgcc gatgctctgg acagtttcga tcgctcctaa accaaaatct gtatattcga
    23761 ttctttctaa ctctgattct ttcataccat tcctgcctca aggtacatct attgtacttt
    23821 tttcttgtaa tccattctgc ctctcctaac taattgtcaa atcatcccaa gatatgtctt
    23881 cttcctcttt ttcttcttgg atgatttctg cattcggatc tactggtttt ttcaataata
    23941 aaatggttat tcctgtgaca aaagaaccag ctaacaatgc accaatgaat gcccatggtc
    24001 tggtcatcgt agcgactgca aacattcccg atgccggaat acggctatct gcgccccaaa
    24061 acatgcttaa tccgccacct actgtcccac cgactcccgt tgctatgaca ttgcgtgcca
    24121 agtcgttcaa tacgataggg aaacatccct ctgtgatctg acagatcccc ataggaaaag
    24181 cggtcttcaa tgtatcgatt tcttgacgat tgagtatatt tttacgaatg attttcccta
    24241 agaaataagc gatcgtcaag ccgaatgggg ttaccatcga agctaaaatc aatacagtga
    24301 ttggttcata gattccttct gaaaatcgtg ccacaccccc ttatccagaa taaatggtaa
    24361 tccagaaaca gccgtgtatc tgcggtaata agcctaacca aacgaaaaat atgagaagct
    24421 atttttgttt ggctgttctt attacttgat gcagggcagc tttttcacaa cctctttttt
    24481 tatttatgtt tcataatcaa tatggtaagg atgcagctcc gtatggtaga ccgcctccga
    24541 atattcaatc atttccccaa tttgcgtaga cgatttccgt atccgtttga tgccgatctg
    24601 tgcgtctgtt ttcagaattc cttgttcttc ttctgttaaa aatactgccg agaaatcatc
    24661 agaaaatgag tcaatagtat atcctttttt cgtcagcaaa cgatacaaag attctttgcc
    24721 aaattcttct ctgctgaatt gcatcatttc ttgtggaaga taatgaacaa aataaatata
    24781 aggctgattg ttcaaataat ataagcgctt aaaacaaaca gcttgctcac cgaaaaagga
    24841 aaacaaagga tcttccttct ttatatggac caattgcaaa tccagcgttt ctttccggac
    24901 ttcatatcgg gagttctcca agatttgcgt aaagcttgcc gctttggata atcgattgta
    24961 aggacgattg cttagaaccg tcgtcccacg accacttttc tttgcgacat attcatctga
    25021 tgccagcagt tcgattgcct ttcggacagt gattttgctg acttgaaaca gttcttctaa
    25081 ttcactttct gtcggcaaca tcgaaccgac tggatatttt cctgagaaaa tatcttcttt
    25141 aattttatct gcgacttcca agtatagtac acgtgtcctt ttcataacgt caccctttgc
    25201 tttactatta ctaaaagaat agcacataaa aaataaaaaa agaacacaaa tcacatgtga
    25261 gaagtgtgtt ctttttcatt cagctgcttt aattacaagt ttacgccatt tgaggcaatt
    25321 acttctttat accaatcaaa agattttttc tttgatcgat tcagtgtccc atttccttca
    25381 tcgtctagat ccacatagat aaagccgtaa cgtttactca tttctcctgt tgatgcactg
    25441 acaagatcga tacatcccca aggcgtatag cccatcaaat ctacaccgtc tgctactgca
    25501 ttagccatcg cttcgatatg acgacgtaaa taatcgatcc gataatcatc aataatcgtg
    25561 ccatcctctt ccatatgatc aatggctcca agaccgtttt caacaatgaa taatggtttc
    25621 tggtaacgat tgtataattc attcaatgcg attctcagcc cttctggatc gatctgccag
    25681 ccccattcac ttgcctcaag aaacggattt tttactcctc ccagcaagtt cccttctgaa
    25741 gttgctgctt gtggatcagt ctcattgatt gccgtagaca tataataact aaatccgatg
    25801 tagtccactg gatattcttt tagcaactgg aagtcttttt ccgtttgctc taatttttcc
    25861 ggatcgactc catatttttt atgggtacgc gctgtgtatg cagggtattc tccacgtact
    25921 tggacatccg tgcagaaaaa attgaattct tggttttgga tcattgttgc tgcctgattg
    25981 actggattgg catcgatgct gtaagttgtt gcatagataa tcatgcagcc gatttggata
    26041 tccgaacgca attcgtggcc gatttttacg gctttgctac ttgcaacgaa ttgattatgc
    26101 catgcttgga aaatattttg gtactctcct gctccattgc tttttactaa tccttgactt
    26161 aaagctggaa agtgaaaggc cgaattgatt tcattgaaag tcatccaata tttgacttta
    26221 ctgctgtatc tctctaatac tgtctgagca aatcgttcat agaagtcgat cagttttcga
    26281 tttttccagc caccgtattc ttttgccaaa tgaagaggca tttcgtaatg agaaatcgtg
    26341 atcacaggtt caatatcata ttttaagcac tcatcaatca gctgatcata aaattctaat
    26401 cccgcttcgt taggagtacc ttcgtctcca tttggaaaaa ttcgtgtcca ggcaatcgaa
    26461 aaacgataac atttgaatcc catttccgca aacaaggcaa gatcttcttt gaaacgatcg
    26521 taatgatcga ttcctcgatg attcgggtaa atatattttt cttgatcaat cgtccagtca
    26581 aattcttcac tgccgataat cgcaaagcgc tgcttacctc caggcatagc atctgcaacg
    26641 gatagtcctt ttccgtctcg atcataagcc ccttctaatt gatttggcag cagttgctcc
    26701 accccataaa aatttttctg aaaaacgtga ttgcatcctg atttcctcct tattttacaa
    26761 ttgccagaac atcttcgttt gcaataatct ctttttggtc taattcaagt atatcggcaa
    26821 atgcatcagt attcgtaatg acaactggag tgattacgct atagccagct gctctaattg
    26881 cttcgatgtc aaaacgaatc aataaatctc ctgctttgac gagatcccct tgtttgacta
    26941 agatctcaaa gccattccca tttaattcta ctgtatccat cccgatatga atcaaaatct
    27001 ccgctccgtc agtcgttgtg atccctacag catgtccagt tggaaaaata gtcgtgatct
    27061 ctccgtctgc aggtgcaact agttctccta cttctggatc aatagcgata ccttttccca
    27121 tcacgccaga agaaaaaact tggtctggta cttcactcaa tggcagtact ttacccgtta
    27181 aaggactttt taaggtatgc cgtgctgaag taatcggttc tcctgcattc gcatgtttgt
    27241 tttctaattg attgtcttgt gtttgttcgt caaatcctaa aatcaatgta cctacgaatg
    27301 caagaacaaa agcaattcct gtagctatta ctgctactgt cacatttgac tctacaccat
    27361 ccaccgtatt aatgaaagtc ggaatcgtca ataagctgac caaactgctg gagaaagctt
    27421 ttactccgga aaatccgata atagctccac caatacctcc agaaatacaa gcggcaatga
    27481 atggtttctt caaaggtaat gtcacaccat acacagttgg ttcagtgatt ccaaataacg
    27541 aagtcactgt agaagaaatc cctaatgctc gaagtttcgt atcttttgtt cgcaatgcta
    27601 cagctaaagc agccccacct tgtgccaaga tggctggaag aagcattggc atcaatacat
    27661 caaacccata ttgttcgata ttcaaaaaca tgatcggtac gaatccccga tgcatgccga
    27721 acatcacgaa tacttgccat aaacttccca tgattagacc tgctacgatt ggactaaatc
    27781 cataaatcga gttgaagaga ccgcctaaag cattaccggc aaccgtcccg attggtccta
    27841 aagcaagaaa tgttaatgga accatcacta ctaaaacgac aagcggaact aaaatcattt
    27901 gcagaaattg cggaatcact ttttttacga atggctctaa tttactttga acccatacgg
    27961 ctagaatgat tggtatgaca gaagaagtat aaccagatgg tgaaagaatc actgggatcc
    28021 caaagaaact gatagcttct cccgcaccag caactgctgt gatagctgga tacacaagtg
    28081 ccatagcaat tgctactgct aaaaactggt tggctttgaa ctttttagct gctgtaaaag
    28141 caagcatgac aggtaagaag gtaaagacac catctgctgc agcaaacaat atttgataag
    28201 taccgctatc agctgtcagc cagcccatca ccgttgccaa tgataagaat cctttcaata
    28261 ccccggcggc tgccatcgcc cctagaaaag gtgtaaatat actagagata atatcgatca
    28321 gacgattcaa aatatttttg gactcttctt ttccatcctc cgaaactgca agaccttcct
    28381 catccacgat cgattgataa acatctgcta cattactgcc aatcactact tgatactgac
    28441 cgccactttg tacgacttgg atcacgtccg ggtcttcttt taacttttgc gtatctgcta
    28501 aagattcatc ttttaatctg aatcgcaacc gcgtagcaca atgtactaaa cttttgacat
    28561 tcttttgtcc tcctaccgct tcccatacac gacgaccaac tgcttgatta tccattgttt
    28621 tttccttctt tcttttttat ttaaaaaata aaaacccaga ttgaaaaaaa cagtcacaaa
    28681 cattcgtgaa cctatttttt caatccaggt tttgcctgcc atacagtaac aatccttatg
    28741 attggatgct ttttttctca cttactactc tttgaatatg aatcgttaaa taaatctgct
    28801 ctgcgatccc catttcctgt ttttgcgttt gtcgaagata ctcattgatc cgttcgacac
    28861 aatgaaacgc ttttggatac tttccttgaa ctaatgcgta gagaaattca tcttgctcgt
    28921 cagatccttc ccctttgaga taacgctggg caaaaaactg cagatgagta atgatacgct
    28981 gataattcaa agattcttca tcgaacacga tgccgaagaa tttactgatg atactcagta
    29041 tatctcttac cattgtggta gcggtcatcg tcacttgcat gtcttcgttt ccttgttggc
    29101 tattcacaaa atgaaaggca ataaaaccgg cttcatcctc agaaagttcc acacccgtct
    29161 tttgtttcac gaacaaaaga gcctctttcg ctgcttgata ttctttcgga taaaattttt
    29221 ttgtttcaaa taataatgga ttaggcagtt ggatattttc ttttgctcgt gtgatcgcat
    29281 aatgaagatg atcggtcaat gtcagataaa tattaggcga aaattcaact tttagtttat
    29341 cttcagccag ttcgactact tgtttgaccc attcgatctc ctctgccgga atatcagcca
    29401 tcagctgctg gaattggttt ttagcaaaag aatctcttaa aacaaattct ttttggaccg
    29461 cttctggcga aatctcgtcc ccaatctttt tttgaaaagc tagtcctttt cccatacaaa
    29521 cgacttcttc cccgaatcca tttttcgaaa tgacgacatt gttattcaaa attttatcaa
    29581 ttttcatttt gcttcgccct ctcaagaaaa ataaaaaaac ctagaattcg cctactgttt
    29641 cttagctgaa ttctggtttt gcctgccgta cagtaacaat ccatttctta ttcaaaatca
    29701 tcataccatc gaatttttat ttatgcaagc cttttcatat aaaaaataaa aaaactggaa
    29761 cacaagtcta tctagaaaac aaccaaatca gagatgcacc tctttgcgtt tctctttttt
    29821 tcagcaaatt ggtttttcct tatcgattca tatcctttgg ctgtttttaa tctgtaacga
    29881 cttatgtccc agtatcctaa aatgtttttg tctctagatt gaaagcagat ctcatatcat
    29941 ctgtcgcatc ttttatcgat gtaacacttt ccagttctcc ttgtacaact agtcgcgtga
    30001 cggcgtccat gtcgccacaa gtaattagtg tgacaatttc ttttccctcg acttcattca
    30061 gtaattgggt atctgtagga gcaacttttt ctttaaaaaa tgttttgtat gtatacacat
    30121 tttctaaatc tgtcaaatag atcttggcac ccatctctaa attttctagc ggtgtaaaca
    30181 gcaattcagg gttcgttgca cgatggcttg ctaaggcata atttcctttt cccatttctt
    30241 gatctggaga taatgttcct gctccgtaaa gtagtgacgt attatccaag cctttgaaaa
    30301 ccggtaaatt gattgaaact gaagggactg cgatactagc aatcactggt agatctgttc
    30361 ctctcatttg cgacctcatg accccttctg tcgtcattgg ttctaccgca tcaaagtcaa
    30421 aactgacatc tttatcgtta ttctttttca gatcttcttt tgttacattc gctatagcat
    30481 atttatctcc tgtctctctg acaaaataat ccttgatctg ttcattgaaa atcaaagcta
    30541 gtcctactaa taacaagagg aacaagagga tattgactag ccatcttttt gcttttcctt
    30601 tttttttcat tttctctccc tccagactca ttttactgtt tttcttttgc tctttccaac
    30661 agccgaatga cacatttcta caactctgtg tatttttttc gtacacctgc gcttttttct
    30721 acaggatatt tcaaattatt tttgatcaat ttttcttcaa tgatctcatc tagatccagg
    30781 tgaaggttgt ctgccatcat gtatgaatag atcaatacat ctgccagttc ttctttgatt
    30841 cgttctaatt gtgtttcttt tacttcttcc ggcgttttcc attgaaaaag ttctaacaat
    30901 tcagatgctt ccaaagaaat agagatagcc aaatcttttt cgttatgaaa ttgtcgccaa
    30961 ttacgttcat ctctaaactg gttgactttt tccatactat tcatttgctt gccttcttcc
    31021 aatcacttct tacttgatta atcataccat ttttttacat aaaaaataga tgatgctttt
    31081 aaaaaaactc ctgttttctt ttatttaaaa taaatgtcat agaaaatcaa taatttaggt
    31141 agccaccatg tgtatgcagt agcgtgtctt cttcccttga ggctttttgt ttttcgatac
    31201 gttcttcttc ccaaacagac aacgctacta gaatctctct taatgatttt ccttcttcgg
    31261 tgagcgagta ctctacttta ggcgggactt ggttataggc ttttcgatgg atactttcat
    31321 cttgttctaa ctctcttaac tgctgggtca gcattttttt tgtgatcgta gggatttgct
    31381 tgcttctcca cgcctaaaat aagcgatatt gctcttccct ttatctggtg taagcaaagc
    31441 agttagcatt ttgacgatcg tgggttccgc ttttcacttc ttatctactt agtatcatga
    31501 agtatacttt cgtactaata ctaagtagcg gaagtgtcaa ctactttgtt taactacata
    31561 gcaaaagaaa cagaagggtg acaacggtca tcacgcttct gtttcttttg aatatgctgt
    31621 gttgttgtct ctatctccgt gtagactttc ctcaaactta tcctttgaca gtaagtcagt
    31681 aggctgattt gctctttcta gatgccagat acatttctac aaaaaaatag tgattctgtt
    31741 ttgaaaattc gatttttagt tctgcatttt cgtaaacatc caacattttt ttcgcactgt
    31801 atagacctaa cccgccattt tttttagagg tttttcctct tttgaccatc tccgatagcg
    31861 tcaaattcgt tggatttgcc ttgttcgcta tactaaaacg tgatccctct ttttcttcca
    31921 gcagattgat cgtaatcgga ctctgatcgg ctacacgatg atcgaatgcg ttagaaataa
    31981 aaatagataa acagcgaata aaaacaacca gatcaatcgg gattgttttc acttcgacag
    32041 gaataactac ttggatcggg attttctccg attctgcttt ttctagaaat tttaaaaaga
    32101 cacccctgac agctggggat acaatatttg ttagctgttg tatttgcggt ttttcaaaaa
    32161 tagctgcaga atgatcttct aatatttcta aaaattccaa cgtttctttt ttattttcct
    32221 gaagagtaat ttttaatgaa aacagtagac ttttataatc atgtttgaat tcctctagat
    32281 gatggtaata ttcttcattt atttgataca gttgaagcga ttcctgatgt tgtctttctt
    32341 ttacttggaa actgatcaac ataatggaaa aaaacaaaga aaaaaaactg atgactatcg
    32401 acaaaagaga tagaagcaga taattagatg agcggaagtg aacacttccc aataccaagt
    32461 aaatcacgcc ccagcttaat aaaatacaaa tacctccaaa atagtgttca gttggataag
    32521 taaaaagcaa gttgagaaat ctcgtctgtt tgtctatact ttttactaat agatagaaaa
    32581 taaatgcgat aagtagtgca aaaaaacttg ctgttccaag atgtctcttt cctaaataat
    32641 ccatcagatc aaaaaccaga aaaaagctga gtccagtgat agcaaaaaaa agattactga
    32701 tcaaacagct gataaacagg tttttcgtag taaaataaaa aagaaacatc aataataagc
    32761 caactaggta aggaaagctc actttaggga taggaagtaa aagtgtcacc accatcaaag
    32821 ctatacaacc aaagctgttt tttactagca gttgtttcga aaagaataaa tagataccta
    32881 aatctaaaat caaagtaaag gtcaaataaa gtgtcactgt tgcgaccatc ttattcctcc
    32941 attgttttgt ttcaaaatgt acacatattt ttttaatagg agcctgcata tgaaaaagta
    33001 ttttatcata gaagataacc agtttcacag cgaatggatc atcagagaat tggtcaatca
    33061 tcatatcgag aatatcgtgc agctcacttc tatctctcaa atcaatcagt tgatccagtt
    33121 gggcactatt gataatcacg atgttttctt tatcgatatc gacttgaagc aatcactcac
    33181 aggtattgat gtcgccgaac aaatacgtaa aaaaaatgca catagtaata ttgttttttt
    33241 caccgcttat acgaattttg cagtagagat catcaatcgg aaaatccttc cgattggata
    33301 tatcagaaaa gacaggaatt tatctgaaca aattcgtcaa gtgctagaac agatcaattg
    33361 gaaagaacag cagatcgtaa atgtcgataa aatctttatt gatcaaaaag gtggaagtct
    33421 cgtcctgtat cctcgggaaa ttttatatat tgaaagtatc aaaggtttga aaaaccaagt
    33481 tatggtcaaa acaactacag aagataaatt agtgaatact tctttaaaac aattgaagac
    33541 agagttagaa aagtatgctt actttttact tttaaaatct tactgtatca acttatcgca
    33601 attgcagaaa attgattctg ctcattatgt gttagaattt ttcaacggtg actccttact
    33661 cgttggtcga aagatctttg aaaagacaaa ggaacgtttc catgacttcc aaaaaactgc
    33721 ttcgagttga tgaataagag ctgagacgta agcatagcag atttcaggga gtgggccttg
    33781 aatcggctcc actccctgaa atcgttgata tgattttacg cttcctcttc tgtaggagca
    33841 tctgttaacg accatgtgaa tgttgaaacg tagttgtcta cagcataatt ggatacagga
    33901 acaaataatt ccactgattg tctcccttgt tccacattct ctcctaatga ataaatccat
    33961 gtgccaatcc cttcatttgc tcctgcagtt actagatcta ctgcctgatc tgctgggatt
    34021 tcgattcctt cagcgccagt tccagaaacg gtagatggca ttccactgct attagttgcg
    34081 ctatttcgaa tcacggcgtt tttgaatttc acctttgaac cagttagttt attcgtatca
    34141 gatgttgtac caataaattc tgttcgtttg actgataatt tccaaccggc ataatttccg
    34201 cttttatccg ttacttgtac gaaattctgt acttgtgttt gtgctccagt actgtccgtt
    34261 ccgctttgta atgcagcata tactgttttt gctgtaccta acttgattgt tccaaaatca
    34321 agatctggag cttggtcaat cgtcagtaat ccggatgttc cctgatttgg ttcgtcaggt
    34381 gtaaaaggag tatcaggatc atttggatca acaggaggaa ctggaacaac gtcttcagga
    34441 tttgcagcat ctggtttggt cacagaaatc gttccttctg tagtggctga gctcttattc
    34501 tcagcaaaaa cactttgtgt acataataat agacttccag ctagaacggt acttaatgca
    34561 attgtttttt tcatttcttt ttccttcttt cttttaaaat aaataatcca gcaaataagc
    34621 caataagaat gactccaaca agtatcagcc atcgccagtc aaatgtttct ggctgatttt
    34681 ttaaatcaat atccttttca ttcaattggt ccgctgcatc atcagttatt tcaaaatcgg
    34741 tatggagatt ccagcgtttt tctttccaag cacccttcac aaccgcttga taatgtcccg
    34801 gagctaattt ttttccttct aatgggacaa gtaaatcaaa atttgtattg ggtgctacat
    34861 ctattttttc tttttttatt gaaaggtaag gctcttttgt ttcttttttg tagatttcaa
    34921 ccgatgtagt caagccgaac atataagaag catttggatt ttgaagattc gccttaatca
    34981 catttcttcc atcgctttga tccgcataag ctttttttaa tttcaaatct ggggaagact
    35041 ctttttcgct atttcaaatc aagattgctt tcacttgaga aaactgattg acgacaccaa
    35101 ctgtactttt gttagaaacg ttctcttttt tttcttgaaa cgtgatccct cctgcgacca
    35161 caccagccag ctctttttct ggcgctttca gtgaaagtgt ctcagtgacc gcactttgag
    35221 gaggaacagt tatttctccc actgtttttg ctaagttttt catatcataa cttaatgttt
    35281 catcaaattt actttttgat tctccatatt cgatcacacc gtttgaattc gtcgtagcgg
    35341 agtggatcaa tgtctcaact gttactgatt tatctgttcg gttaaagacc gtgtagggga
    35401 tttcctgtgt tttcccaggc tccatcctta catttatata tgttttgttc ttatcaatct
    35461 gattagccgg taattcaaaa tctaccgcaa aattgaattc ggaggcatgg acaggtgtct
    35521 gccaaaggat gaatccaagg agaaggatca taagtaaaga gcagttgttt cgatgacttt
    35581 tattccatat tcgattcatc aatcatcctc cttgatcttt attctgtcgg cgcatctgct
    35641 aatgtccatg ttatctctgc tgagtaatta ccagctatct gattttcttt ggttaagaca
    35701 agtttaaacg cccgaccgtt atttaaatat tctgaataat ctgcttgcac attcgttgaa
    35761 ttttgtcctc tgacaatttc cagttcttct tcattcacta attgatactc ctctcctttc
    35821 ttgaaatata ggacatcctg catagaaaat cctgcatcat ttgtgaaagg ctgagataat
    35881 tttatcgtta agatccacgg atttttcgtt tctcgctgat cataaatcca taatttttcc
    35941 tgtatatcca tttggccaaa tgcttcttgt gtcaaaccag ttgctttgat ctgaccaaaa
    36001 tcgagtttgg aaggggcaag aaatgagaga ccaccttcta ctacgatctt cgtagcaggt
    36061 gcaaaaggcg gggcatcatg ataaacaact ggcgtatcac ttggattgtg attcccaatt
    36121 tcatcgttgg tacttggctt gttctgcaca cccttagcag tagcttctcc tgcttggtcg
    36181 tttaatacaa tttctaatac atctcctgca aaaagttgat tttctgggat agaaagaaca
    36241 aattcccctg tatcatcaat agttgtttct gacaaaacag cttgattttt tttcaatgaa
    36301 accaaggctc ctgcttcacc tgtacctgtg atcgtgcgag aagctgcatt gatcaatgtg
    36361 gtacttaaat tagcaggttc aggaggaatc acatcgactg tcgttttggc agagtccagt
    36421 tctgtcactt cttggccacc tcttactttt ttagcagcaa caacttgcac ttgatcctcc
    36481 gtttttaatc tctcatcatt gggtacttga acttcgaaca agccgccttg ttcgccttct
    36541 ccccatgggt tatatcctgc cccttcattt tcatctgttt gtccttgcgt gagagcagta
    36601 atttctacag atgatttcgt cgtatctgcg taagtaatac gtaaagtcac ttctacttcg
    36661 cctgtccaag catctcttgg ctttgcctcg acaccttcgg gaacaacgac atgcccatag
    36721 atttttttat ctgcattcgt tggcactctt aaggattcaa tcatcgggag ctgattattg
    36781 gcactgattc gattataatt tttcattccg gcacctgtct gagcaaattc atttgttaaa
    36841 cctgcactag ggcttgatac gaaattgcct aagtcggtcc cattgatcga taagtctgtc
    36901 atatcagaaa aataatccgg gtctttttca aagttatcga atgttgcgcc tgtttttcgc
    36961 cataaagcga tttctgaatg ggcgatcttt aaagaagaat ctccatttcc ttggaatacc
    37021 cagccgccta tgccattggt ctcgcttcca atccgtttgt tttggaaatc aaaataggta
    37081 ggagattcta agtccacatt caacacatcc ccactgatga tgccggtctt atcttcccct
    37141 gttttcccga ccatgactaa ataagttttt tcacctgccg taaaatttaa atcaatatct
    37201 ccagttgtat ctacagttgc tccggaatct gcgcgtatat cgatatttga tttttctcca
    37261 gtgactgtaa aatcagctga acctggactt tctagaaaac cagaagcgtc ataaaattgg
    37321 atcgcttgat ttccttgatt gagtctgttc acgtaagatt tgccatcacc tgtatttctt
    37381 accctcaatg agcctccatt ggttactgtc agcttttgcg taccattctc aaaacgaact
    37441 gcgctggaac gcccactctg cttatcgatg tcgataactg atccggctcc ttctacgatc
    37501 aaagaaaggc cttttcctac aaaacgcatc ccattgttcc cgggttgatc gtcatctcca
    37561 gccattttga ccgagatttg tccaccactt gatacatgga tttgactaga atctccttgc
    37621 aagcgaaatc cgcccgtagt tgctgtattg atggtgattt tcccaccatt cgatacagtg
    37681 actttcgatc ctgaacctct catttggatg attccgcgat tgttgtcatt ggtaatattg
    37741 atctttgtat ttaccgaaaa ttcgcttccc gaaccactga cttcaaacat gacattacca
    37801 ggtagcgtat cgactgcctc tccttcatcg ctaacgccat ctgcccgatc ataatcaaga
    37861 gagacctttg aaccgcttaa gatatggatt cctggcgctg tttttccact accagtctca
    37921 tctcctgaat ctagaaaacc gtagaatttc cctagatacg gtccataaag ttgactacca
    37981 ttgatcacat tcaattgata gcttctacaa aaataggctt tacccggagc ggcattcagt
    38041 ccttccttca ctggttggac atccaacaca ccggaaacac catctaatgt gacagttgca
    38101 cgaggagcgt aaatcagtcc taatttggac ccatcataca aatcaagtgt cccggtaaat
    38161 tttatttctc ccgtaaaatg atatctgctt ccccaaaaat cggtcatata agcaatgata
    38221 atggcatttc catcgatatt tggtgtcaat ccacctggat gtgcagctgt tcctttaaaa
    38281 ttctggaact cgatcgactg aggtatctca ttgatataaa tatggtgtgt gccgaaattg
    38341 tgagcgaatc caccaaaatc aataacgata tctcgtttga tctctaattc cctgtctaaa
    38401 gtcaaaggct gaatcagtcg gatcttagtt actgattcgt ctgcgagaag ctgcgccagt
    38461 tcttctgttg tggcagcttc gctgtaccca tccgctgaaa cagctcttgc cagtgccgct
    38521 tcactgctat cagacgtttg ttcagctact tctgaggtct cacttgtctt attttcttct
    38581 tgccttttat tagtagaagc ctcactttga gattctgtag tttcagaagt aatcgattct
    38641 tctgaaaaag tagtcttgtc aaaactttga ttctctgatt gcgttgattc gtttgttttc
    38701 tctacatcgg caaaaacgga tgtcaccatc aagagaggga agacaaacaa aatcgacgca
    38761 atcagctttt ttacacattg attcattctc caccatcctc tctgacgttt tgacaatttt
    38821 attttatcgt tttaatctat ttccaattgt actttcatat ttaaaatatg tatttttatg
    38881 gaaaaatttt tctattaggt gtaaatttct tttttgtaca aaaaaaaggc tgtgacaaaa
    38941 gtcttgtcac agtcccttat cccgaacaag cggtgttctc ccgccaactc ctcggtcaca
    39001 acctccattt taacaataaa cctgtaaatg tgagggcaat actttgattg tcacaggtaa
    39061 tttatcccct tcatctccat caacattcgt cgtcaattct tcctcatcag ctaatgacac
    39121 aactaattgc tcacaagtaa ggtaggctag atttttcgta gattcatcta ctccttttaa
    39181 taggtctggc aatgttttaa gcgtatcgat caaagaagaa tcttttaaat aaaccaaatg
    39241 gagcttacca tcatccactt ttgcatttgg aagtaatgtt tcaaaaccgc cgactgagtt
    39301 agttagtcct atcagcaagg tactgctctc aatttcttcc ttctttccat caacttctac
    39361 gtgaaacgag taggattgtg tggaagctaa ctgtttgatt cctgacatga aataagctag
    39421 ttttccaaat ttcgtcttct tctccgcatc tacatcattg attgattcag ggatagagcc
    39481 aattgcgacg acattcataa aatagtcgtc atttattttt ccaatatcca atggcttgac
    39541 tgattcaatc gaaaaatgat tgatcgcttc ctcaggttct agcggtatcc ctaatgctcg
    39601 agccaagtca ttcaccgtac ccaatgggaa aaatccgaaa tttggccgat gttcttgttc
    39661 agcgattcca ctgattcctt cgttgactgt tccatctccg cccatgacaa acacactatg
    39721 atacccttcc gtagccgctt cgcgagtaaa gttttttgca tcccctgctt tttttgtatg
    39781 caagacgacc acttcatcaa acaccgattc caattttgct atcgctaatt gctcaaattc
    39841 ctttgcttgt tcaccgcctg aactggggtt taccactaat aaaacttttt tcatcacttt
    39901 ctttcccttc tgattgattt ctttaatata acgatagcat aaagaaaaaa attaggctgt
    39961 gatgaagtac tgttcatgaa acattcattt attttttgac aagataaaac atccatacta
    40021 tgaaactgag gaaaattaaa acaatgatat aggaaataaa agaaaatgat aggcattgta
    40081 tacctaataa agtaaaaatc ttaggtggca gataaaaaca caaaaatagc aagagaatgt
    40141 aatagcgttt cttccacttt ttgttatcag accttatagc gatcagagga ataccaaatg
    40201 cataactgaa aaactcttga tgtactgtta atatgtcacg gccttgaata ataaatgcga
    40261 caatccccgc tataattagt gtgcctaaca ccaaaacgga aaattgatga atatatttta
    40321 tatacaaatg atccattgta cttcctgcct ttttatttgt gagaaaaaca aatccgtata
    40381 cgtgaataac ttctagaaat aaaagaaatt tgcaaaacca ctccatttat ctacttctac
    40441 gtgcattttt tgatattgtt ttctttattt aagattatac agaaaaatat ctagggtcgc
    40501 tggattctaa gtaaaaaaaa tttttaaaat aaaaagaact aaatcaagta gtaattaaac
    40561 tactgttgat ttagttctaa taaaaagatt tagatgaaca gttttattga tttaaatagt
    40621 tcatattttt tatttatacc gcttatttac tacttgctgt actagaatct gaagtgaatg
    40681 tactcaaaac attttcaaag tctttgtctt tgatttttac attagcgtct ttcaatactt
    40741 cgccaattac tttattctgg aaggtactgt cacttaattt tgtgtctgtt gcaatttctt
    40801 tcaattcttt tttgtattta tccatgtcgt tgcctttgtt ttggtttttc accattttaa
    40861 ccacataata ttccgtagta taagttgaag cattggtaga agtgatcaca tcagaaactt
    40921 gaccatcttt caacttaaag gctgcttctt tgacttctgc cggaaccgtt gtagtagtgg
    40981 aatcaaattt cacttttccg ccatcttctt ttgtcgtagt atcagttgac ttgtctttgg
    41041 ctaatttaga gaaatcgtct cccttatcag cagctttctt cacatctttt gcatcatctt
    41101 cacttgctac ttggataatt tgagcttcca cttctggatg gaaagattcc caagccgttt
    41161 ttaaatcttc atcggtcaat ttgatatgtt tctttagacc ttcttgaaag gctagacttt
    41221 gtttgatact gtctttgaat gtttgttctg tgtaacctgc agatttcaat tgtgaatcaa
    41281 atgaatcacc caattgtttc tttgtttgat catattgaga atcgatttct ttggttgata
    41341 cgtcatctcc atatttatct tcaaatactt gataaacgat caactgttgc acagcggaat
    41401 ctgctccttg catttttttc aagctatcca ttacttcttg ttcgctgatt gtaccgcctt
    41461 tataactaac ggctgttttt tcaccggcat tcgtacagcc tgacaataag ccagtgccta
    41521 gtacgatggc aagtgctaaa aatctttttt tcatttatca attctcctac ttttcgtttt
    41581 ttgcgaattg ctaaaaacaa aggtgttcaa gatgaaaatc atcaagaaca cctttactaa
    41641 tctgctcgat acgtcctgaa tagatataag gctacacaat ccagaaagtt cgctcagcag
    41701 aataaatgtc aagtagcaca tcacacctta atatactact tcttttgtta ccgtttttca
    41761 agtctatctt gcgttccgta tttaacgtaa ctaattctta gaacaaatgt tatcgatttt
    41821 gtttttcttc ttccgctgct gggatattca aatcagacac ttttatgttc gattttccag
    41881 acattttttt gttgttttta ttatgatata tttttataca actaaaaatg aatatattaa
    41941 aactaccaaa aataactttt aacgctgttt cattatatgt tttttctatt ttatataaac
    42001 ttttggatac ttaataccaa ccggttggat tgtaccaaca tgagaatgtg tcagttctat
    42061 ttcctgtcgc acttgcatag gcccatactc cttttgttgc atttgctgat cgaatagagt
    42121 ttccaacttt cgcttttgca ccgtgacgat tatagacacg actatagtgg ttagaatgac
    42181 ctgtttttct cgtacaagtt gtatcactat aataaaccca tcttccagag ctatgattaa
    42241 ttgctgttct agaaatttcc cggtctactg gtaaatttga cttaataata ttacctttag
    42301 catcaaaatg aatctctcct ggctttaaag atttgtaata gatttcaccg tgaactgaat
    42361 ttgcttccac ttcagatgac atagcgtttc ctccaacaaa acctatccct agtgcacaaa
    42421 taataactgc ttttttcata aaacgacatc ctttcctttt tgatttttaa agcatgatct
    42481 tcttgtttcc tattttggtc agatatggat catgcgtgac tacgataact aacgtacctt
    42541 gggaagcttg ttgcgataag atttcaacta ctttatcctt atttctttta tccaatgacc
    42601 cagtcggttc atcggctaaa atgattttgc tattcttcaa taagactctc gcacaggcga
    42661 ttctttgctt ctcaccacca gataatgtgt agataggcgt acttagcttc ttgtttatat
    42721 ttactttttt caatgcctca tactttgctg ttcgcttatc tgaaacattt aaattcgaaa
    42781 acttcaatcc aatatctaaa ttaaaatcga ttgtttcata ttcaatcaac ccaaaatctt
    42841 gaaaaagaaa agatatggta tttcttttta tcaagcgctt ctctctccca gttaattgtt
    42901 tcgtctcttg atcgaacaaa tagaattgtc ctgaactgaa ctgatcattc aatcctaata
    42961 tattcaataa cgtactttta cctgctccac tttctccaac aacagtcaca agatccccag
    43021 catccgcagt gaaacgatcc aaatgaagaa tcgttttgtt tcgaatgatt tttttcgcac
    43081 cttttatcac tatcaacggc tccaattgtt aacctccatt catcagcaat aactgacttt
    43141 tcttttctgt agacaaaata aaaacaactg aaagaataaa atcaatgaag ctaaagaaaa
    43201 aaatattaaa aagatattta ggctctcttt ctataagaaa aaggaagatt acacttgtcg
    43261 ttccccattt gatgaggttc atcatgaaga aatctttata cgtttcaaag agcgaataac
    43321 cacttgaacg aaagatagcg attttccgac aattgtagac aaaatatatg cataacatga
    43381 catagctcaa caagaagaaa gaaaagaaag tgagtaaaaa tatctgtatc aagtaatgaa
    43441 atgatctgct taacactttg acagctatct cttcagtctg agtatacgga aatgataact
    43501 gcaagttatc acgtaactga ctttcttcaa tcagctcaaa taacctttct ttcgacaaag
    43561 agcctatctt gatctttaac ggtggataga tttctccgct gagaatattt ctttccaaat
    43621 acatactgtt tttcaatgtc agtatttcaa caaccggata atgttcgatc caggggatag
    43681 aaggaataaa tgtataaatg ggttgattgt tttttataaa tagaaatttc ggttcttttt
    43741 cactgatttc ttgataatat tctgttattt gcgccaggtc attttttaaa tctgtatgtc
    43801 tatatcgttc agggatcaat ataacagggt gcgtttcttt ttcggttatt tggatcaacc
    43861 ttccagagac atcaatcaaa ggaaatttct taagataatt cagatttatt tttatccctc
    43921 tgccaaactg ttcattttct tcattcaagt aatctgatac attgactaat atacttccgt
    43981 ttttattcaa agtttcatat aattcctgat ctgctgctga tgcaaaacga ttggaataaa
    44041 taaaatccac gtggtttttc ccgatggtta ctggataaaa tacatgatag tcctcaccag
    44101 cgatttcttt tggtggttca tgggaaacag ctaactgtga agaagaaaaa acgatagttg
    44161 ctaattcgac attggcaacg gtcaaaagaa gagaaaacaa caatccaaat gtaaaaagaa
    44221 tgtattggat atagttgaac gcttctctta acgaagcgat tttttcaact aataaaagag
    44281 aaatcaaaga gataccatat attccgctga acaagaaaac gtacatctgg aacaaataaa
    44341 gaaattgctc cttataatac ataaaaataa aaccaccaat gattcctgca attatgaatg
    44401 taataatgat tttctttttc cccaataaac accacaatac ttgatgtaat gaaaagtcat
    44461 tattcaacaa taaactgatt tctttattca tcaataagaa aaaggatgat aacaaaagga
    44521 ataaaagaat caatccaata gttaaataca ctcgataatt atatacgtct gtatttgaaa
    44581 taaccgaata cgaatagcta ttttcaaagt cttcaaaaat attagtggca taatcagtgt
    44641 gaaatgcttg attgaatcga tcagtgaata gtgaaatgac ccgttcatag gcttcttttt
    44701 ctctcgtcat caagaaaaat tcaccttcaa aatcttttat tttgatcgat tcttcaatgg
    44761 acgttacgcg aaaggaggta ttagaatagg gtaccttctc gtgagaagat ttgacatccc
    44821 cttgataata caatgtcatt tcaagctttg gctgtatctt gaagttccaa gatgtatcta
    44881 cttcattgga ttcatttact gtttttttca tataagaaag ctcagtttct gatgagacat
    44941 tttcaaatac catgattagc tgctcatatt cttcttgccc aataaaatgt tgtggcaatc
    45001 gaatcggagt ataagtatct gtttgatgga aaaattcatt tgtccgatag agtacctgtt
    45061 tatttatact aaccaaggca aaaccactca tgataccggt cactatgatt gtgagtaaaa
    45121 ctgtgaactt ttttttgatt atcaccactc tcccttactt aaatgattct ttgataaaat
    45181 cataccgttt ttcatcaaaa aaacgtatct atcaagtgta taggtcatgt agaataaatc
    45241 tatatactgt agaatcgaaa ggagaagaag atgaacaccg gagaaaaaat acgaacttac
    45301 cgcagaaatt gtaagcttac gcaaaaagaa ttaggcgaaa aaattggtgt ttccgataaa
    45361 acgatttcta gttgggaaaa ttctcgtaca atgccagact ttgaaatgct gtcactttta
    45421 catcatactt taggtctgcc gattgatttt ccttctattg aatcagctac cgaatcaata
    45481 gaaccaacat tgccagatga acctcttgtt aacaagaaat cttattcatc aaaacaaccc
    45541 ttctcactca aaaagacact atctttcttt ctgctcgtct tttgtttggt ctatcttttt
    45601 cttatcaatt atcaatacaa aaatgaaacg atcgatcgat tcaatccttt tttgaaacca
    45661 caaatcggct ataccacact ccctgtagga tacgtggaca attccatcga acgttgggta
    45721 tgtaatgata tctttggcca tggtacatgg ctgtcttttc acgctggaga ggtagaaaaa
    45781 gagaaaaacc atgctgtaat caaacacaaa ggaaaacacg tcatgaacgt tcgcctgctc
    45841 acttgggaag ccattccctc agattaccaa gaaatcctca gccaagacta tgacgacgcc
    45901 ttaaaaagaa gccctcagga gccagatagg cattaagtag gagatgaacg tagagataga
    45961 aaaaatcaat gcacatgatc ttatccaaaa aacaggggta caaattaatt gaaaaagcct
    46021 tgagatgaaa gttcctaaat aaaaaagttc agaatgatag gtatttctgg cttatagtat
    46081 aatatttacg ccacagcctc ccaatgattc tttttggaat cataatcaag gatggacatt
    46141 cacaaaaata ttttgctcat tcgatccgaa tagattaacg accaatctag taagtaatat
    46201 agattggtac gtagtcaagc tgcacataat ttagatcaat tttagttctt ttttatattt
    46261 ctatgatctg ctcatttact tttatatgat ggcttacagt aacataaata aatatgataa
    46321 tcgctggtag tttatccaag agtactcaat aaaatacaaa atggattgtt caagcgccta
    46381 tcataatgat aaaatgaaac aaataaacga tacaaggagg aagagacttt gagtaatggc
    46441 agaacaattt tttcattgta aaaattgttc agttgttaat ctatgggatt tcggtagcat
    46501 gggcattttt ttatagagga atcacaccaa cctggccgtt ttatctcttg ttagtgggaa
    46561 tattagtagg aaattttgcc cctcttcact atcgtacgaa cttttttcca actgattctg
    46621 gcctttttct taaaacaaag acgcgaaaat tagaaaacat tattgaaatg acaattacct
    46681 gtataatcat tttcttagtt atattcctaa acgtcttcta ataagaatct cttaaaatta
    46741 gcttgttaca gttagttacc tagtttatat aacacagtta tcttatcttc cttatcaaca
    46801 tcttttgcca actctttgtc ttaaattcga aacatataag cagagctacg aacaccaaga
    46861 ctgtttcggc tctgtttttt gcatcaaatt cactcttata tagctgataa tctcggctct
    46921 ttaaaagaaa ttattttcgc ttcattttat tataaaaagg ctaggaacct tgatgttatc
    46981 aggttcctag cctttattta agtattaccc cacagaccct tccatataga tttttaaatt
    47041 acacactagt ttaaactaaa acaaacaagg aaaaacttac catttaatca aaaaattagc
    47101 tcatatgaat atattagaat gtaaattaga ctggtcaaaa tctggtcaaa aaagaggacg
    47161 aatacaggta gtcctctttt ttgttatttt gatattctaa atcatttttt attggattat
    47221 tcccttgttc gatatagacc caaattgatc tatcaataat tatcaaactg agtcatatag
    47281 agacaaaaat ttcaaaaatg acctgttaaa tggtcatcga atatcattta aaatcaatac
    47341 gacggagtca gacacggagt caaaacaagc ccgctgagag taaaaaatat acagtcccat
    47401 aggtaaagtg ccacaggact gtatattttt ttatttgaga ttacctgaaa tgattccatt
    47461 taatttttat ctcatttttt cctactagtt agtatcaaat actctcctat tttctcaata
    47521 aactattttg ggttacatgg tatctgaaac agctaaaacc ttgtcattat agcaaaaaat
    47581 cactttatct ccgtttcatt tcagatacat ttcacttaag cggattccaa aaaaacaaaa
    47641 gaaaaaggaa cccctagtta gcctatgctt tttattggtt cctctcaaaa cacggtcata
    47701 taacaacggg gtggggtaaa tgaatgaaaa aggtggagga aacattattc aatagctgtc
    47761 ttaaatactt cgttataaaa tgtgaacaaa taaaagacat ctatccctat tttctacact
    47821 tccacttgac taccatcttt gaatcgtaca attttcttaa ctcttctcgt ttacctcaat
    47881 tcattctcaa gtggaatcaa ctcatcatct aacattttac gccatgctcg cttcatttct
    47941 tgtgggtatt taaatggctt cgcttctgga tgtttggctt tatatccttc gtaatcctta
    48001 ctgttgataa ttgctttcat attaggaatt ggatcttcac ctggtagata ttgcagatta
    48061 gaagcatgta attctttctc agaaacaaac cactcattgg aaaagacatg aatcaaatca
    48121 tcactcgctt gtgtaaaata gcgacgttta atgacaaaga tatcttcatt actatctaaa
    48181 gtccccacat caaatgcatt taaaatttgt cggtaaactt ctttgactcc ttctgtttta
    48241 ttcaatttag ccaaagcttt ttcaatttct tctcgtgcat ctgaactatt cgcagcataa
    48301 gttcctagta attgatcaat ataagttgaa tcaatatcat acaacttagt ggtattttca
    48361 ctataaaatt cgatcgttga aaaatcttct ccttcttttt ctggttcttc ctctattaat
    48421 gaacccttca cggtctcgta cacgccaatt tgttcttcca aaagttgaat ttgattgttc
    48481 aaagttggag atttcaccat gtcatcattg taatcgtcat aagtcactaa cgattcataa
    48541 gcacgattca attcttgaaa tgctttgacg tactcgatac ggatttcaag tggcgtatgt
    48601 tcatcaatgg attccatgga tgaaacctct tttaacttac catatgcttg tttgaatctt
    48661 tttttggatt cctgataact tggataaatc aaacctgatt cttcttttgc ttttgaatac
    48721 agttttgtcg catcctcgac attcttctcc attgtcgctg gcttacgaaa cgtcacaatc
    48781 aatccttttg tcttatcagg atacgtacga ttcgtacgtg aaaaagcttg gatcaatcct
    48841 gcatactcta gattacgatc cacaaatagg gtttgaatcg taggggcgtc aaaacctgtt
    48901 aatagacgat ccactacaat cgctaaatcg atttgtttcc caaattgttt gaattccgct
    48961 ctttttcgtg ccaaacgatt attgatgtct ccgttataac gctcaatgtc tgctaatgac
    49021 caggaagtat cataatactg attatattcc tcaataattt tttgcatttc ttcttgttgg
    49081 gcacttgaat ctcggctatt ttcttctaat gaataggtaa tagcgattcg tggaaaatca
    49141 ggatcctcca tcgttcgtcc ttcacgaatg agctgattgg gaaactcttt tgttaaccaa
    49201 tcaggctctt tcgtcatctc tttgatggcc cgataatact ttttggccat gtcaatggaa
    49261 ctcgtggtta agattgcaga tttcgttggc ctgccatttc gaaaatcaaa ttttgtatag
    49321 gcattatctg gacggaacat tttatgaatg actttttgga tatgttcatc tgcttcaaaa
    49381 acagctgggt ccaaataact ttcttttttg acaggttcca tttgatcaat catacgattg
    49441 atctgttctg atgaatactc tgcatatgtt tctacttctt ttaatttccg atagatttta
    49501 ttctctagtg aagtcggctc cacggtgttt tcatgctcta cttgaaatcc taagacagag
    49561 ccatcttcta gcgcattctt aatggtatag gtatgcaaga cctctccata ctgatcatgg
    49621 gttgtacgtg ccaattgccc tttggcttgt ttccgattct caggaaaaat cggtgtacct
    49681 gtaaagccaa accaagtgga tttcggaaac atttttttga tttcttccat attttcagca
    49741 cttaacgctc gatgacactc atctactaca aacacgatat gttggcccat taatttttgg
    49801 aagcgattgg ttcctttttt ctcttcttgt ttctttgcat atttcaacgc cgcatctagt
    49861 ttttgacggg ttgtaatgat tacaacattt gcattggcat ctgccaacag ggtttcactt
    49921 aattctttgg cacttcccgt tccaacgatc aagctattcg ctttggcgtt cccggaagaa
    49981 atcccagtat taaattccga agcaaatttt gtaaactctg tggtggtttg attatctaaa
    50041 tctttgcggt ccactaacat gatcgttcga tcgatccccg attttcgtgc taaaagctta
    50101 gtagatacaa aactagtcaa cgtctttcct gaaccagtcg catgccacac ataccctgat
    50161 tgatgtttat ttgcggcaat aaacaaggct tgaatcgcat gtacttgata aggatgtaac
    50221 accatgaggg ttttattatc ttgatcttca ctgacgattg tataatccgc aatcaaacga
    50281 tgagcatccg ggatatttaa cacttgttta caaaactcat ataagttttc tactttttca
    50341 ttgtctttcg ttcgccaact aaataaaaac ttagggtgca agtctttcgg taaggcattt
    50401 gcaaaatacc gagtggtttg ttcattggaa accacaaaca actgtaaggt cgaaaagata
    50461 ttgttccgaa acataccttc ttctgcatat tttttgattt ggttaaatgc ctgataaaca
    50521 ccatcttttg cagttacttg tttcagctcg atttggacaa tcggtaatcc attgattaat
    50581 aaggtgacgt caaaacgtcg atcccgtgct tctatgttac ttccacgttt tgcaatttga
    50641 tggaccactt cataagtaga gattcctcca ccaatgtctt gattggaata aaggataagt
    50701 gagacagaac ccaattgcga atcttcccgt tcaatcgtga tccgagccat cccattttca
    50761 ccttttaacc atttcgcagc atcaaatggg gttttcgttc gcagcagtaa ttccgttttg
    50821 atcgtttcaa attctttatc ggtcaaagga aaatcattca gttctgcttg attatttgag
    50881 ataatttttt ggcgtaaatt gacccaaagg tcttcttctg attttagatc cggacgatac
    50941 gtccactgat tatgcccttc tccaagtaca cgaatcaact gttcttcgac ttctgcttcc
    51001 tcacggtgac taattttcat tcttcttccc tcctagacaa acatcttttg taagaagctt
    51061 tttttcgttt cttttagtaa atctaactta cgttgatgaa gagcgatcgt atcatctagc
    51121 tgtttgaaga aagtaccgat tttttgttgt tcatccttta caggaacctt aacactgata
    51181 ttctcaatat cagaactgtt ataagaaggc aatgccccta cttgggagta tcttggcaaa
    51241 tttatagttt caaacaatat tttaccaaaa tagatatccc aagtattatt aaataaataa
    51301 gccatggtat tgttatctat taagaaaggg cttgtgacca aacgttttct attaagcatt
    51361 attgctgcgc caactttagc aaatataatc gctggcacat cttttattgg cttccatttt
    51421 ttacgctcaa tttgctcatt agatacataa tgatttgcat ttctcatttc atactcatta
    51481 ccaatgttat tcatgtcgga aactttaaaa aatggagtac cttcagaacc accttgctcg
    51541 gcatcaggaa atccaattcc cgattgagtt ttacctaatt ctcccaactt acgctcttcc
    51601 caatcttccg taaaaccagg aaaccggatt tctggcactt ttgctccgtt ttcagggaac
    51661 attttttgta agaatccttt tttcgtttcc ttcagcaaat ctaacttacg ttgatgaaga
    51721 gcgatagtgt cgtcgagttt tttgaagaat atgccgattt tttgttgttc ttttaaatca
    51781 ttgggaatat agaatactgt atctgaaact tttttccagt ctgaacgagg catttttgtt
    51841 ccagatgtat catttgcagc cttttgatat ctatcagctt gaataagaga ataaataaag
    51901 tccgagtcgc tcttcttagc tctaaaaacc cagaaatccc caatcgctac gcctgtaaaa
    51961 gttgccttga gccaattttt taaatatgga cgtaactttc catatagaat atcatttggt
    52021 aaaaattcaa tcccttttct attatcaaat ttctggctca catctttatt cagtctacct
    52081 tctcctgcca cgatatcttc aaattcaact tttggaagtc tactggagtt tgtactttta
    52141 ttaagtctaa caactaaatc aattaactta cgctcttccc aatcttccgt aaatcctggg
    52201 aaacggattt ctggtcgtgt atcattgctc atgtttgaac acctccagcg caccttgtaa
    52261 ccacatttcg ttttcaggtg ttgtttgcag ggaagagata gcttctaaaa gttctttttc
    52321 tagcacttgc tgttcttgtc gtatcttctt gatgtcattt ccgacatgaa ccatatcaat
    52381 tggttcttct tcttcgaaag tatctacata gcgtgggata tttaagttat agtcattttc
    52441 tttgatttca tcaaaggttg ccaaatgagc gtatttctcg acatcttttc tttcagcgta
    52501 ggtttcgagg attttttgaa tgttttcttc cgatagttta ttttgatttt tccctttaac
    52561 aaattcacga ctagcatcaa tgaataatac atctcgagtt tgacgattct tcttcaacac
    52621 aatcaccgtc gttggaatcg atgtcccaaa gaacaagttc gcaggcatac cgatcacagc
    52681 ataaatactg ccatcttcta gtaatttttg acggatgacc ccttctgcag cgccacggaa
    52741 taaaactccg tgtggtaaga cgatggccat ggtacctgtt tccttcaaat gatagaaccc
    52801 atgaagaaga aacgcaaaat ctgctttgga ttttggcgct aatttcccat aacgattaaa
    52861 ccgagaatca tctaagaaag tcgtatctgc tgaccaatta gccgaataag gtgggttcat
    52921 tactactgca tcaaatgtgt aaggttcatc tgttggccag tccttattca acgtatcccc
    52981 attgcggaga ttcatttctt cggcgtcgac accatgtaaa atcaagttca tttttgctag
    53041 attgtatgtc gttgtattta gttcttgccc atgatattta acgttatctg ggtgtgttaa
    53101 gtagttacgt acgtttaaca tcaatgaacc tgaacccatc gttggatcaa aaacactaaa
    53161 gaatcgtcgt tccttttgat cgagcgttac gatttgcgcc atcatatcag aaaccatatg
    53221 aggtgtgtaa aattctccgg ctttcttccc tgcttctgaa gcaaattgac tgatcagaaa
    53281 ctcatacgca tccccgatca cgtccccatc gtgttctaaa acatctacat cattcaattt
    53341 cttgatgact tcggtaatcg tcacatttcg ttgttgctca tctgttccta gtttttttga
    53401 ttggagatcc acatcatcaa ataacccatt aaattgattg taggtagatg ccaattgcac
    53461 aaaggctttg tttagatcgt tcaattggaa cgttgcttgt tttgcttgat ccgctaagac
    53521 gttgaaaaga tactctggtg caattgcata tcccaaaata tcaacgattg tggcaatcag
    53581 atcttctttt gattcttcat ctgaaagtaa ttcacgatac aacattgttt gtttgcttac
    53641 tgtgtcatat tcctctaagg attcatctgc taaaagaacc acttgttcca acaatcgatc
    53701 ggataaatat ttatagaaaa tcagtcccaa taaataattt ttatattctg acgcatccat
    53761 tttactacgg agattatctg ccgcactaaa taatttttga tttaattctg cacccaaaaa
    53821 gatacactcc tatctcttat ttaaagttgc tatataccct cttattttag cataccccct
    53881 tttataaaaa gcaaaaaaaa aagaaagaaa cttattcttg ctaataaact acgcgaaagg
    53941 agtctaacgt tgtttgatat aacttagact gatcgttaac ttatttttta tatttctaca
    54001 tagttatctt tagaaccatt tcagatacat atgaaacaat tacatatgat tttttcataa
    54061 aaaaacagta ctgtctagta gtataaaata tacaaatttc ttaaaaattt tttcactaaa
    54121 atcactacct gaaacagtct aaaacactta tatatcaacg tttaatgaaa ctatctccgt
    54181 ttcatttcag atacaaataa atatagtcgg gctccaaatc cgaaaaaaaa tacccccagt
    54241 agcttatcct aagctactgg agatgtataa gtacggtcat ataacaacgg ggtagggtaa
    54301 ataaatgaaa aagatggaag aatcattatt caataacttt tttaaatact tcgttgtaat
    54361 cagttacctg tccgttctta aataccacag aataatcttt ttacccattt tcaagtaacc
    54421 gaagtggaat gtctttacca acttgctctg tcactgcatt actgatacct gttagattag
    54481 ctaataaatc tgataaagat tgagcatctg tagaaatctc tgtatcagta tttatcgtga
    54541 ttcctttatc ttcatttaca agcttccaag taggtggtat agctgtgtat gtttcgatga
    54601 atgaatcaat tctttcatct atcgtcttcc cagtatctac ttctgtctca ttactcttag
    54661 atgaatcgat tctttcatcc atcgtctgct ccgtaactgc ttctgtctca ttattctcac
    54721 cggaaactct cttcacaccc aaacttatat attgtccatc tttagtgtgc atagttaaaa
    54781 ttcccacgct tttactaata aattcactat aaccagttct ttcaatagag acttcaatgt
    54841 atttcacact atcatactca aaagaaaaat agacatgtaa aagcattttg ttgtttacat
    54901 ctacataata atcctttgac tctacttgag accttcctat acggaataca gctttactgt
    54961 ctgatatctc tataccagca tccttattat taatctgcca atctccaagt agctcttctg
    55021 gcaagctagt agtttcttgt gaacacttta ccacttcact ggtagaggtc gaaacattca
    55081 cctccgcact gttagaagaa ttttccaagg aatcaggagc cttagcacaa ccaacaagtc
    55141 tttatatatt gtgcagccga tattttcttc attcagtaca cccttgaaat atgcagtgtt
    55201 agataaagca gttcgtcctg atttgctgag gcacccatct cttcttgaac gtactccttg
    55261 attttcaagg ccgcccgata ctctttcgga aaacgctgct tcactgaagc cagcaaaaaa
    55321 tcatccttct ccggcaactc ttccttctta atcattcggg caataaaaaa ctgtaaatgc
    55381 gtcacaaatc ttaagtaaag catcgaatct ttatcaacct ttttttcggg aaacagatat
    55441 cctagcatag ccaagatatc ctttagtgct cttaccgaga gcatggtttc ttccatattt
    55501 gcttgttcac tttgggcatt tacaatgtga aacgcaatgt ttccagcctc ctcttctggc
    55561 aactgaacat tcattttttt gttgatcaat tggatagcat agcagccgat ctcaaattct
    55621 tttgggtaat acttttgaat ttcaatcagc aggcgatttt gaaaaacaat gcctttttga
    55681 ctgcgttcaa tggcatgctt caagtgatca accaaggtaa aaaatagact tttattcagc
    55741 ttaaagtcaa acttggcgtt ggcataatcg ataattcgct tgacgcaaat aattagatca
    55801 tccggagcag cctcaaacag tttaatgtaa tcctttttta gagcatcaga aagtgaatct
    55861 aatttaaata ctttttggat tttgtcagcc ggaaccaaat cgcctttctt tagttgaaat
    55921 ccaatccctt tccccatcaa aattacttct ttgccttgct tatctaagca aagaattaaa
    55981 ctgttgttta acgttttaat tactctcatt agattcaccg ttctctctaa gctaagccat
    56041 ttcttgcgat tacttttcga taccaaaaga agctttcttt ttttattcgt tttaattctt
    56101 taagattctc atcggttcgg tcaacatata taaaaccata acgcttattg aagccttgat
    56161 gggtactaac aacatcaatc gctgaccaag gtaaataacc aaaaacatct acaccatctg
    56221 ttaaagctgc ttgaatagcc agaatatgtt tggctagata atcaattcga tcctggtcat
    56281 taattcgtct atcttcctca actatgtcgg gtgccccata gccattttca gtaattaaaa
    56341 taggtaaatc atagcgatcg tacaatttcc tcaatgttaa tcgtagtcca gtagggtcaa
    56401 ctacccagcc atatggcgtt ttatctacat aaggattttc agctgcccgg tagacacctt
    56461 cttctcccaa catgatctgt tgatcccccg ctctagcgga aacatctgat ccgtcatttt
    56521 tgctggcagc gatggtggca gttgaataat aattaatcgc aataaaatcg ggtttaccgg
    56581 aggataaaat tgcctgatcc tctgcctcaa ttttaggata taatccacga tcattcaaat
    56641 agttttcaaa caaataattg tattttcccc gaactgctac atctaagaaa gaccagcctc
    56701 tcaatgtttc ccaattatgg gctgcaattg catcttctgg actggaggta gcttgataca
    56761 tagccgttaa attgattgct ggaccgattt ttcctttaag attcaactga tgatacaagt
    56821 gaattactct agcttgagct aataaaacat ggtgattaat ttgaaagagt tctttttttg
    56881 aaggcagctc tccacttggt ggcaaaccaa tcgctccagg atgaaggatc atcgtgtttt
    56941 gctcattaat tgtcagccaa tagttcacct tgtcaccata ctcagtaagc aatacctgta
    57001 catagtccac aaaataatcg atagttgctc gatttaacca accgccctgc tcctctagaa
    57061 aataaggtaa atcgaaatgg tacatggtga caatcggttc gattccctga ttcaggcatt
    57121 catcaattac ttgatgataa aaagaaattc ccttttgatt ggttttatta ttttcaatat
    57181 ccataattcg cgtccaagcg atggaaaagc gaaaagcttt taagcctaat tctttaaaca
    57241 gctgtatatc ttctttatag tgatggtaaa aatccgaggc aacttcaaaa ttagctaccc
    57301 cttctgtatg ctgatacttg tcaataatag acggttgttt cccatcctcc tgataggcac
    57361 cctctgcctg gtaggcacta atggaggcac cccataaaaa cttctctgga aatgctgaaa
    57421 gctgtttgta ttgcacaaaa atcctctcct taactaattt gcatcaatgg ttcgtgttcc
    57481 tttaagcttc cgcttgcttc actaattcct gcgtaatttt cactgttggt cactaccaca
    57541 atcactgtat catcgtagcc agcgtttcta atggcagcga aatcggcgtt gattaataat
    57601 tgtcctgctg acacctgatc tcctgctttt acaaatactt caaagggttc cccttttaaa
    57661 ttcacggtgt cgatccccac atggatcaat aattcaatac cttctttaga acgtagacca
    57721 atcgcatgtt tagattctaa aatagtaatg acttctccct caacaggtga ataaatgctg
    57781 ttttcttctg gatgaatggc tacgcccttt cccaatagtc ccttagaaaa gacagcatcg
    57841 ttcacttcag aaataggaca cacggttcct tttaccggat tttcaacaat gactgcttcg
    57901 gctattgccg tttgctctga ttcatcagaa gtatcgtcaa atccaattag ataagcaatg
    57961 ataaaggttg caacaaaggc gattacacac gtaataattg cgtgaacaat attcattgga
    58021 ttttctccaa taaaggctgg taaggctgct aagccagggg aaacaaaggc atatctgact
    58081 aaaccgctca agccagcata caaaccagca attgcaccac caatcatgga agcaactaat
    58141 ggttttttca gcttcaaggt tactccatag agtgaaggct cagtaattcc cattaaagca
    58201 gtgatcccag tggacacagc tagctgcttc aactctttgt ttttagtttt cgcggctaca
    58261 gcaaaggtag cacctgattg agcaatatta gaagccagca ttcctggccc attgatcatt
    58321 tcagaacccg tactgctcaa ttgcatagta gcgattggcg tcattgccca agctgtaccc
    58381 gtaaccacca aaaatggctg caaaccaccc atcaaaaatg gaattaacca agctgctttg
    58441 ccgttaatcc attcagcccc cattgctata agatcgttca gcaacgttcc aaacggtcca
    58501 atgacaacca acgctaaagg ccccataatt aaaaatacca gcatcggctt agtaaagaat
    58561 ttaataaacg aaggcgaaat tcgatccgca aagggctcta cataggacat gatccaaaca
    58621 gtaaagataa tcggtaaaac tgatcccgca taatctgcag ctaataccgg aattcctaga
    58681 aaagaaacgg catcgccagt agaaaaaagc tgtgaaatat ttggatgaag caaaatgcca
    58741 gcaacagtaa tcgccaaaat tgggttgaca tcaaacttta tggctgcccc ataggctaat
    58801 agtactggca taaaataaaa ggctgcatca gcaatagtat tcaaaatgat gtaagtttga
    58861 ctactgtctg atagtacacc agtcaaagtc aagacggcca aaatggcttt taccatcccg
    58921 gcacctgtta tagctggaat catcggtgta aaggtggtag aaatgacact aatcaatctt
    58981 gaaataatac tttcttgatt gtctgaagag gggccctttt taattgaagt tcccacttcc
    59041 ttttgaattt ccttgaaggc tgtttggact tcattgccaa tcaccacttg gtattgtcca
    59101 ccgctatcca ccacactaat tacaccaagt gttttttcta aggctttcgt atcggctttc
    59161 cttttatctt tgagttcaaa acgcagtctg gtggcacaat gggttaagct gaccacattt
    59221 tcttttcccc caacctgttg caatacattt cttcctactt ctctgtaatc cattattctc
    59281 tctccttaaa gcagtaaaaa agaccaagtc aaaccacgcc ccaatcaaca ttgaagtgtt
    59341 agtttgtctt ggtcttgcct gcataatcag taacatgcca ataacgttat tttgctgtaa
    59401 tccaataata gcacattatg atagcgcaat caacagggaa tttaattttt tatcttttcg
    59461 tttgaccgta tagggtaata taacagttag atcacatcaa ctagggtata tctgcgtata
    59521 cttactgcta aaataagtat atctaatatt aactatacca gtaaaaccat tcctacataa
    59581 ccagcgggaa attcccaaaa tcgtgaaagc atcaaaaatg catataacgg atcacacaac
    59641 ttcaacaatt ctgctcttgg gaaaaattga tgaatttggt gagtaaagga tggaataaac
    59701 agcattccaa caatataggc aacaatagtg atacttgttt ttttggtaaa aatcagtaag
    59761 caatagccaa tttcaatcat tccgagatat aaaggtatca atagaatgat ttgggctagt
    59821 attcttaaaa attaactagc aaatgtttaa actgatttat ttctaatcta catgtagtaa
    59881 gtatttcaat tccaccaatt atcaataaaa ataccaaaca tatccataat aattttttct
    59941 ttttcatttc ttttatattg ttataacagt gaataacata aaaattcccg acaatcattg
    60001 taatattttt ttgtcacctg tattagtatt taatttatag gaaactataa aaactcccta
    60061 gtgaatttac acctttggga gttcctgttt ggacggtatg cataatacat ttaaatcaat
    60121 ttaacattaa cacctcagca tatggtatct ccacatatat acggtctttc tttggatagt
    60181 actcaatatt tatgttgata gcacttccat tgtctaatat agctttagcc cggtatgtgt
    60241 tatctccatt ttcatgagta atcgtcacat catcacatga tcgatttaag ttgtactgtt
    60301 cttgtaaaat ctgtgttacc aatggttttg atgacttctc taatccagat gtacctgtca
    60361 tttttgaaaa aataatacct ataatagcca agacaacaat acctataatg acaaacttct
    60421 tgttcatatt tactttattc aactttttat ctcctatctc caaaataatt tgttcttatc
    60481 cctcttatca gtacattgac atccctaaaa gtacaccaaa gattgctcca gctacccctg
    60541 ctatcatcaa aatcgtaccc attttctcat ctttttctcg atataagtag cccataataa
    60601 cagcaacagc accaaataca acaggaataa atgctaacga tactaccata gaaatccaac
    60661 caataatcaa atacagcttc cctgtctctt gtccatgtac gatttgttcc ttcatttcat
    60721 taattgtctg ctcagtactt ttctttactg tgtctgttcc ggttgtctca tctgtttctt
    60781 gtagtgctcc acaaaactgg caatacttac tttcatctgt tatttctttg ccacattttc
    60841 tacaatacat gtcttctcct cctaaaatca ttcaattgct tattagttaa ctgtttactt
    60901 tattaaatac atcagaaaac agctcattca ttggtacact taatatctca gaaagcatct
    60961 tcatttgata gggtttagga atcgttctcc ctgtttgcca cgaacttaca cattgctgtg
    61021 taacattcat tttttttgct aactccgttt gagaaagtcc tttttctttt ctgtatcggc
    61081 tgagattact tacttttttc atgattctcc cctcctgttt tattatatac aacaatatga
    61141 tgtatttcaa ctcataaaat ataaaaaact tcaaatacca ttacaaatac aacaacttgt
    61201 tgtactatac tgagatgggg gtgtattaat gaaacagaaa caatatgcaa aggtcattag
    61261 agaaaagcga aaagctctcg ggtggacaca aaaagaatta gctgaaaaaa tcttctctac
    61321 acaacaggca gtagcaagat gggaaaattc tgtaacagag cctaacctcg atagcttaac
    61381 agctctttct agagccctag gaacccctgt aagccatttt ctagacaatg ttgtcgtaga
    61441 ttatgaggaa gagtttttgg ctctctatcg ttctcttagc actgaagatg cagttcgaac
    61501 aattgactat atgaagctat taaaaaggca agaaaatgaa cgcaatcagc tattgaaaga
    61561 ttgaaaaata aataacccac cgaactttaa cggtgggtgt attgagatga aataagatta
    61621 agagctatgg ataacctgtc tagtttaata caattgggtc ttttctcttc cttactctcc
    61681 gtccatcatc tcttgttaca cttgtaaatt gatacagagg aactatccca gctcctgata
    61741 atatcaattc ttttattgtt tcatctatcc ctgccccttt cgctaaatat attaaaaaac
    61801 tactacttgc tttcacattg tatagtttct taatatcctt agaagctcgt tcaagagtga
    61861 atccttgttt aacaaagtag cctctgtttt ttattaaagc atttttcacg ctgttatccc
    61921 caacattacg taggcgttca caaacagtgt taataagttc tttctctgtc attagatatg
    61981 tcccttgcca cgataagccc tgtaaaactt catgtgcttc atctaaaatc aattgttttt
    62041 ccttgctgag ttctgatcgg gaattttgag gaaatactct tttagcctct ttttcgttaa
    62101 aacatatctt aacccattca taagaaaaat attgatatgt aaaatgaatt ttttccagaa
    62161 gtggaaggat atcttcattc aatttttgaa tcacagaagt tgcattaaaa acgtctaatc
    62221 gatagacatt gcttctctta tgattaaagt ttcctaacgc tttctcttcc tttaacctat
    62281 ttaggtattc gagcatcttc aaaggaacat cttcatcatc taattttttt atcaatccta
    62341 taaaagtcat aagtgatagt aacttattac atttatctat gtcataagtt ttaccatagt
    62401 aagaactcat ttttttagct aattgttccc ccgacaaaat acaagtcaca ctttccttac
    62461 tatcatcaaa aaaattaaca tcagaaagca agacatcata gacacgtcgc atttcctcta
    62521 gataattcct tattgtcctg aaaagtttgt ctttcattga ttcaagagtg tcaaaaagta
    62581 cttttgattc ctctttcaat tttcgaaaat gtgtaggata caagtctagg ttctctaata
    62641 gccaattaaa tgtttctacc cgactgtctt tctttaaaat tagcatcaca actccaataa
    62701 aattttcact gatatcacaa ttactggaat gacaataaaa aatggaatgc ccagttttac
    62761 tggtaaaaat actagcagag ggattatgat cgttgtgaaa cagacatctt aagtttttac
    62821 ttgacttaag taatttattc atatcaatgg ttaacaatct ttcataaatc tcattaaaag
    62881 acatgtcgga ggaatcaaac aggcagtctt taaaccactg tttcatttct tctttgtcgt
    62941 cattttttat caacttaaca aaatcggttt cattgactag ttcatactct ctccctgaat
    63001 cactttgaca atttttagaa catgtcatgg taaaatcaat cggaagctca gtaatatcta
    63061 acatgttgtc ttcattaatt attatcactg catctgactt atatcctcca aaaaacattc
    63121 tattcgaagc atttacattg tcatcacacc cggggatgtt ctttttcaag tattcgtaaa
    63181 cagcaaccat tttaccatag tcagtaattg cttcttttaa tagaaagaca atcttgtatt
    63241 tttctagatt ttcagagtac cgaatacttt tttgtatcgc aaacgcagaa ttatgaacta
    63301 aattgataga taaaaccttt tctatgctta agtatctact atcatcttta ctcaaatcta
    63361 ctaccttccc cttctcatct ttaacagtgt tatcgacatc cagaataatt actctggttt
    63421 ccacaaaact ctccttgtca ttgcctttat tctttgtctt gcttggaata aatgattgtg
    63481 atgaatttag tatctctaca aactcatctc ttgtaacttc tctatagtta tctaaacacc
    63541 tatctcttac atcagttttt tctttttttg taactggctt ctctaataat ttttcttgat
    63601 ctacataaat tggaatatta atcattgatg tttccatttg tgtttcctcc ttataaaatt
    63661 ctgttaaaga ctaaaacata cagataataa aaacaaactc tttattacta tatgttttag
    63721 tcttatttgt ttactcaacc tttataccgt tccatgaaac tatcaatatc agatttttta
    63781 aatctaatga ccgtatcaat ttttataaca ggtaacccgt attgagaaac atattttcta
    63841 atcgttccta ctgatacacc tagatattca cttgcctgct gatagtccat atactctaga
    63901 gtgactccag aaataatgtc ttctagcata ggctttagca ctgtaattat ctctttcact
    63961 tgttgttcaa cttgtataga aacaatgcta tcaatgattt gtagagacat atttccccct
    64021 cctttcgtga ttgcttgaac aaattttcta agaattatct tcgtattttt tgctcaaaat
    64081 aaaagtattt tgttattaac cgattaatca aacaaaaaat gattaccagt tcatcgacat
    64141 acacataata cctcgataaa aaaggggtgt caacagcttt gtaaaattta tttacaacac
    64201 cccttttttg aggtatacta attttaggag gtgtggtcaa tatgataaaa cgtccaccaa
    64261 taaattattt agaacgaaaa aagatactcg gaacaaaaat aaaagctata agaaagagta
    64321 aaaaattaac acaaccagca tttggtctta tgattaacaa cggtcaatta attgacaaaa
    64381 aaactatcta cgaatgggag aagggaacct atttaccaat accagagcga ttatcccgaa
    64441 ttgctgattt gggaaatatg agtatagagg agttagtttg tggcaatgta gaagaatata
    64501 ttttgggaat tattctttat agagatagca ttgtactaga tggtataaca tttccagata
    64561 aaaacctgtt ccagcatctt cgtcaacagt tcccaccagt acactctaat ttagatacat
    64621 ggctggatag atattccaaa ttagaaccag agatgcaaga atttattgct aacaagactt
    64681 gtaataaagt taaaaatgaa aaaattagtc ttttcaatat cttaaaaata gaagaattat
    64741 tcatcaatgc tattgtagaa gaatttgaca ataacattct tttcctcact agcagtatag
    64801 aagaactact agaaagaatg gtagatgaat ggctacctat tcaattgaaa gacatgtctt
    64861 atcccgaaga agctgtgaga gaaattactg ataatataaa caagctagaa caaacgattt
    64921 catcaattgg gaaaaaatac actaagaaaa aaatgaaggg aggtgatacc atctagtggt
    64981 aagtactcct tgtaaactaa gaggagatga tttaatggca aaaatatcaa agtacaaaaa
    65041 gaaaaacggt caagtggcgt ggatgtataa aggacacatt gccaccgatc cacgaactgg
    65101 tgaaaaaata aatactacga gaagaggttt tagtagcaaa caagaagctc aaaaagatta
    65161 tgacgagtac agacatcaaa tattatacgg tgttaaaaag aaagcacctg atatgacatt
    65221 tgaagacctg tataatgaat ggataactca tcagcgtacc agtgttaaag cttcaacaat
    65281 cgcaatctca gtaagatacg caaataatca aatacttcca gcatttggta agcttaaact
    65341 atcgaatatc tctgtcccat attgtcaaaa agtggtagat gaatggcaca gtaaatatga
    65401 aagttacgat tatatgagaa aacagactgc acaaatttta cgctacgggg ttgcaatgca
    65461 atatattgac aataatccta tggaaaaaac acttcttcca cgaaaaaaag agtatgagaa
    65521 aaatcgtaaa ttttactcaa aagaggagct taataattta ctggatgctt ttaaagattt
    65581 tggtaacatg aaacaatacg ccttcttcag attattggca tacacaggta tgagaaagag
    65641 tgaagtactc gctttacagt ggaaagacat agacacgttt aataaagaac tacatgtcaa
    65701 taaaactctt gctgtagatg aatttggtaa agttataatt caatctccga aaacaagagc
    65761 ttctagacga gttatctctt tagatactga gactctttct atcctcaaca actggaaatt
    65821 acagcaaaaa gaagagtatt taaaacttgg atacaatact agctctaaag agcaacatgt
    65881 ttttacaaca gttaaaaaca ctctatacat tccaaacact gttaatgatt ggttaagata
    65941 tattttgaaa aaatataact tgcctcgtat tacacctcat gggtttagac atacacatgc
    66001 tagtttatta ttagaagcag gagaatctgt caaggtagtt caacaacgat tagggcatga
    66061 aaatagtaaa gtgacattag acatctatgc acatattaca aataatgcac ctaagaaaac
    66121 aggtcaagac tttgctgata tgatggctca tcagtaaaag agctggtcaa aatctggtca
    66181 tttctagtat acaggcaaaa caaaaaagct agaaccctta tatatcaagg gttctagctc
    66241 ctataacagg tttacccaac actgccttcc atctcataac tgatcaatcg attgagttcg
    66301 actgcatatt ccattggaag ttctttggtg aatggttcaa caaagcccat gacgatcatt
    66361 tcagttgctt cagattcggt caggcctcgg ctcatcagat agtagagttg ttcttctgag
    66421 atttttgaga ctttggcttc gtgttctaaa gagacttggc tgttgtggat ctcattgaat
    66481 gggatcgtat cggatttcga ccattcgtcc atgatgatcg tgtcacattc gatatgagag
    66541 atcgaacccg cactgttttt ggcaaaggtt acttgtcctc gatagtttac ttctccgccg
    66601 tcttttgaaa ttgatttaga gacgatagag ctagatgtat ttggtgcatt gtggatcatt
    66661 tttgcacccg tatcttggat ttggtttgct cctgcaaagg cgatggagag catggtgcct
    66721 cgtgcacctt ttccatctaa gtaaacactt gggtatttca tcgttgtttt tgcacctagg
    66781 tttccgtcga tccattcaac ggttgcgcct tcgtatgctt ttgctcgttt ggttactaag
    66841 ttgtacacat tgtctgacca gttttggatc gttgtgtaac ggcaatacgc atcttttctt
    66901 gtaaagattt ctacgatggc ggcatgcagg ctgttacttg aataagtagg tgccgtacaa
    66961 ccttcgacat agtggatgct tgctccttcg tcaacaatga tcagcgtacg ttcgaattgc
    67021 cccgtgtttt ccgcattgat ccggaagtac gtttgtaatg gaacatcgac tttgacgcct
    67081 tttggcacgt aaataaaggt cccacctgac catacggctg aattcagcgc tgctaatttg
    67141 ttgtcggttg gcggtacaag ttttgaaaag tactctttga ataattcagg gtattctttc
    67201 aaagcagagt cagtgtcagt aaagacgatt cctaactttt cgaattcatc tttcatatta
    67261 tggtacacga cttctgattc atattgtgcg gaagcaccgg ctagataagc tcgttcagct
    67321 tctggtatac caatcttttc aaatgtatct ttgatttttt ctggtacgtc ttcccagtca
    67381 cgagcgggtt tgtcacttgg tttttggtaa tatttgatgg catcgaaatc gatgtctgaa
    67441 aggtcggggc cccatgtttg cattggcatt ttgttaaatg tttctagaga tttcagacgg
    67501 aattcaagca tccattctgg ttcgcctttg attcttgaca tctcacggat cacttcttct
    67561 gtcaagcctt ctcctgtact gaaaaccggt tcgacatcat cgtgaaaccc gaatttatat
    67621 tcttctaatt ctggtacgcc catgttctca ctcctcttcc ttgtggtaat gatggaaagc
    67681 tgcggtacca tcgttttcca ttgcttttcc tagtgctttc caggcaagtg tcgcacattt
    67741 gatccgagcg ggaaatttag ctacaccacc aagcatagca gcatcgccta attggtcggg
    67801 atctttgact tcttttcctt ggacaagctg tagaaagtct acagttaaat gttctgcttc
    67861 ttctattgat tttccgatga cggcgtctgt catcatcgag gcacttgccg tactgatgga
    67921 gcagccactg ccgtcaaagg cgatattttt gatgattccg tcttttacat cgacttgcaa
    67981 ttcgatcaca tctccacatg tggggttgtt caattcgatc ttgcttgtcg agtcagtcaa
    68041 gcttccacga tgatgcggat gggaagaatg atccaagatc acttgacggt ataaattgtc
    68101 taatttagat agtcccatgt tggaaaaact cctttgttgc catgattgct tcaactaaac
    68161 ggtcggcgtc ttgcgttgtg ttgtagaaat aaaagctcgc gcgagcagtc gctgatactt
    68221 caaggtactt caatagcggt tgcgcacaat gatgacctgc tctgacggct accccttcca
    68281 tgtctaaagc tgtcgcgaca tcatggggat gtaacccatc caaattgaat gcaacgactc
    68341 ctgtccgaaa attcgggtct tgtggaccat aaacagtcaa tccatcaatt gcttgtaatt
    68401 ttggcaacac ataagcggct agttcttctt catatcgatg gatattgtcc ataccgagtt
    68461 tttgcaaata atcgattgca tggcctaatg cgatagcgcc ggcgatattt ggtgtccctg
    68521 cttcgaattt ccaaggtagt tctttccatg tactgtcgta taattcgaca aaatcgatca
    68581 tttcgccacc aaattcaaca ggttccattt cttctaacca ttttcgttta ccatataaaa
    68641 caccaatacc tgttggtccg cacattttgt gtccgctgaa agcaaagaaa tcacagtcta
    68701 gtgcttggac atcaatggcc atgtgcgggg cagattgagc accatctact acgatgaccc
    68761 cgccgttttc atggacaagt tcagctaatt cttgaacagg attgaagaca cctaagacat
    68821 tggatacatg agcaatggag acgattttgg ttttgtccgt aatctttttc gctgcatcct
    68881 ccatatccag gaagccttct tctgttagtt cgatatattt caagacagca cctgttcgtt
    68941 ttgccaactg ctgccacggg ataacattag aatgatgttc catgtaagag agcactatct
    69001 cgtctcctgc tttgatatag cggtcgccga aggttctggc tacccaattc aagcttgtcg
    69061 tcgtaccacg agtaaacagc acttctgctg tttcttttgc atggatgaac gcccgtactt
    69121 tttctcttgc ttgttcataa tcatgggtcg cgcgttctgc taacgtatgg acaccgcgat
    69181 gaacattggc gttatcgtgt tcgtaatagt acgctaatgc ctggagtact tgttttggct
    69241 tttgggtcgt cgctgcatta tccagataaa ctaaaggttc atcattgacg tcttgaaata
    69301 agataggaaa atcattgcgt agctttttag catcgatcat ccattcaact tcccttcgat
    69361 tacttcgact aattctttcc gaacttgttc tacagggatt gctgttaaga cagaaccgag
    69421 gaatcctcga atcactaagc gctctgcttc ttctttatgc aatcctctac tcatcagata
    69481 gtacatttct tctggatcaa ctcgtcctac actggcagca tgtccagcag ttacttcgtt
    69541 ttcatcgatc aaaagaatag gattcgcatc accacgtgct ttatcagaaa gcatcaagac
    69601 acgactttct tgttgggcat ctgcaccttt tgcccctttc aagatgtggc cgataccatt
    69661 aaaggtcaat gttccttttt ctcggatcac tccgtgttgc aaaatatgcc cgatcgtatg
    69721 aggggcttta ttggttactc gtgtatcgat tccttggatt tgtctgcccg agctgattgc
    69781 cacgattttg acttctgcgt gtgcaccttc tcctgctaaa tctgagtcaa agtcagcgat
    69841 cacatgaccg tcattcatga caccaattgc ccagtcgaca gaagcatctc gtaaaatgtg
    69901 cccacggcga ttcatataag aagtaatatt ttctcctaat tgatctacgg ctgaatattt
    69961 gatttttgaa ccagctttag cgatgacttc cacgatgata ttacctgatg attttgctac
    70021 ttgttctttg gttgtctgga aacgttccag ataagaaaat tcactatgtt catctgccac
    70081 gatcaacaca tgcttgaaga agtgctcatc ggatgcgcca tcttgaatga ataaggactc
    70141 gagtggttca tcgatcacga catttttagg cacatacagg aaaataccgc tgttcataaa
    70201 agctacatgg agtgcagtca attggtcttc gttcatgttt actgcttttt tcatataata
    70261 ttcttggact agttctggat attcgatcat tgcagtgaac aagtcggtaa aaatgactcc
    70321 tttttctgct aattccacag gcagttgttc aaaaatcgtc catgaacctt gttggacaag
    70381 cacaggattg tctttcattg catcaaatgc cggaatcgtg cctgtttcac tgatggtttg
    70441 tgtttctttc acatcaaata acgaccagcg atggaatttc acccgatcga tatgcggcaa
    70501 tggcagttca tctgctttcg ctagtgcgtc ttgacggaag gttctcatcc aatcaggttc
    70561 gcctttactt aaagaaaaat catttaccgc gtcaagataa tcgagccagt ttttttcttt
    70621 cattcttgct cccccttacg cttcttcttc tttatagtcg attcctaatt ctttactgat
    70681 accagcgtag ccttctgctt ccagacgttt agctaggtca gcattccctg tcatgactac
    70741 tcggccttcc atcatgatgt gaacgacatc aggtgtgata tagttcaata atcgttgata
    70801 gtgggtgatg atcaatgcac cgaaattgtc tccgcgcatt tcatttactc ctttagcaac
    70861 gactttcaaa gcatcgatat ccagacctga atcgatttcg tctaaaattg caaatgtagg
    70921 ttctaacatc aacaattgca ggatctcatt ccgtttcttt tctccgccag aaaaaccttc
    70981 gttcagataa cgttcagcca tttcttctgg catatttagt aaagccattt tttcatctaa
    71041 ctttttcaaa aatgccataa cagaaatttt gtcgttttca tcacgtttcg cattgatcgc
    71101 tgcacgcatg aattccgcat tcgtgatacc tggtatctca cttggatatt gcatagctaa
    71161 aaataagcca agtcgcgcac gttcatctac ttccaagtct aaaacatttt tcccgtcaaa
    71221 caaaatttcg ccttctgtta cttcatagtt tggattgccc atgatagcgg cagataaggt
    71281 agattttcct gtgccgttag gtcccatgat ggcatggatt tctccggttt tcattgttag
    71341 gtttacgcct ttaaggattt ctttttcctc gatcgacaca tgtagatttt ttatttctaa
    71401 gacagacatg cattataccc ccaaattctc tttcgttctc actcatttta gactaattga
    71461 gaatgaagcg caatgacctt ccttactaaa atcaaatatc ttttatttat tattaaacaa
    71521 ttttgatttc taaattagaa taggtataaa aaagatgaga aataagccat tacagactta
    71581 tttctcatct tttttgttcc ataccttatt ttgcatcagc ctttactggt ttaacagagc
    71641 cgccccattt gtctgtgatc cattcttgga cttctttgcg gtgaagcact tcaatcagtt
    71701 ttttgatttt gtcactgtct tcatcacctt tacgagtcgc aataatattc acgtaaggag
    71761 agttgtcctt ttcaagcaat acggcatctt ctaatggatt caaccctgcg ccgtaagcga
    71821 agtttgcgtt gattgcaact aggtcacctt catcattttg gtacgtagaa gctaaaagtg
    71881 atgggtcgat cgtatggttg aacttcaagt tcttagggtt ttcttcaata tcatcgaatg
    71941 ttgctgtttc cagatctact ccatctttga ctttgactaa gccagcatct tgtagaattg
    72001 tgatgatgcg tccccagtct gattcagaat ttgaagtaat gattgttgca ccatctttta
    72061 attcggaaac atctttgact tttttagagt acaagcccat tggttcgatg tggacagcac
    72121 cggcattgac aaagtcatat cctttttctt tgatttcttt gttcaaataa ggaatatgct
    72181 ggaagtaatt ggcatcaatg tcgccttctt ccaaagattg gttcggtaat acatagtcat
    72241 cgaatttgac gatttcaaga tcgattcctt catctttcaa taatggtttc acatgttcca
    72301 aaatctctgc gtgcggtgtc ggtgaagcgc cgactttcaa tgtcgtgtcg tctgctttat
    72361 ttgctgaatc tttgctgtcg ctgttgttac cgcatgctgc taatcctaca gtcagcaaaa
    72421 gcgctgctgc taatccaaaa attttctttt tcatatccta catcccccta tatcctatta
    72481 tcttttgtct gttttcttag ttaagaaatc accgattcct tgaacgataa atacgattag
    72541 taaaataatt aaagtcgcaa cgaatgtcac tgtcagatta ccacgctggt agccttcctg
    72601 ccaagcaaga cctccaagtc cacctgcgcc gatcgcgccg gccatcgcag taaatccgat
    72661 catcgagatc gttgtaactg ttaatccaga aatcaatgcg ggaatacttt caggtatcaa
    72721 gaccttccag atgatttccc agtaacttgc tcccattgca tctgctgctt ccagtacacc
    72781 agggctgact tcacgaaaag caatctctac tagacgggca taaaatggtg ctgcggataa
    72841 aacaagagct ggaatggctg cttgcgcacc tagcatagtg cctacgattg ctttagtaaa
    72901 tggaatgatc aaaaccatca ggatcataaa tggcgttgac cggaaaatat tactgatgat
    72961 cgatacgatt ccataaatga ttcgagcata cggtttgttt ttgcgtccaa tagagtaaag
    73021 gatcaatccc aaaactaatc cgatgatcgt cactaagacc attgagatta atgtcatata
    73081 tagtgtatca aggatagcct gattcatcgt ttccgggttg atttgtttaa aatctagata
    73141 tgtttctgaa aagcttttat ccacgatgga tcacctcaat tcctactcct tgttctttga
    73201 tgaacacttc tgcttgatct aattctttgg tttcaccagt catcaagaca gtcaatgaac
    73261 caaaggaacc ttctttcact tgtttgatct ttccatacac aacgttgaca tcgatgttat
    73321 agcgtcgaat cgcttgggaa atgactggct cgttggcatt tctttcgcta aaggtcaaag
    73381 ttgccaaacg gccgcttgga ttttctttga tgagttcagc taatagttct gtcgaatcag
    73441 cttctggttc gatgtcttgt tgtacaaagc gtttggttac ttcttttttc ggttgtctga
    73501 agacagtcaa tacgtctcct tcttctacga ctttccccaa ctccatcaca gcgactttgt
    73561 tgcagatttt gcggatcaca ttcatttcat gtgtgatcaa gacgatggtc aaattcaatt
    73621 tcttattgat cgctaacaat aagtctaata cttcttctgt tgtttgcggg tccaatgcgc
    73681 tagtagcctc atcacaaagc aggatctcag gatcatttgc caatgctcgt gcgataccga
    73741 cgcgttgttt ctgcccgccg gaaagttgtg aaggatatgc ttgtccgcgt ccttctaacc
    73801 cgactaaacg caacagttct tctgcccgtt ctttacgttt gctctttggt acgccagcta
    73861 attccagcgg cagctggata ttttcaagta cagttcttga ccataacagg ttgaaatgct
    73921 ggaagatcat accgattttc tttcggaatg tacgtaattc tttgtttttt aattttgtaa
    73981 tatcttgatc gtttacgatc acttcgccgc tcgtgggaag ctcaagtcca ttcaggagac
    74041 ggactaatgt actttttcct gcgccagaat agccgacgat tccataaata tccccttttt
    74101 caacgtttag tgaaacgtca ttgacggctt ggatgtttcc gtttttccca ttgaatgtct
    74161 ttttgacatt cgttagttgg attaatgaca tgccattcac atctttctct tatgattttt
    74221 caaacaaaaa agccttcgcc ctcttgcctt aagcaaaagg acgaaagctt gaacttccgt
    74281 gttaccacct tgatttaccg ttacttcgca ataacagcct cttccagtac ttggacttag
    74341 cttctttccg tatactgtcg cgctgtaacg ggcactcccg tagtaagttt gcttacgctc
    74401 gcttactctg ctctaagacc atcttccatc tcattttcgt tacccatttt cagcaaacag
    74461 ggctctcttc aaacgtccgt gacatgtact cttcttttca aagctttctc gtttcatata
    74521 ggattgctta tgattatagc aactggaaga atcagtgtca acttttttca agtgattttc
    74581 tgattttata attcgtcaaa ttcttttgga tcaacatctt tgaaacttaa gatccacgca
    74641 ttcatttcat ctgaatcatt caagatattg atatcttcac cgattttatc attaacagaa
    74701 gaaactgtac ccgttagcgg gctggcaaat tcattgaccg ctttttccgc ttcaacttcg
    74761 atcagaggtt catctttttt tagcgattgt cctgcttttg gcagattggc aaacgtgatg
    74821 tctccaagtt cttcttgcgc ttcatttgtc aaaccaacac agtattcttt tccattaaat
    74881 aagatccaaa ggttgtcctt tttcttcaaa tgcttgcctg ccattttata tcagttctcc
    74941 ttcataaatt ttttcattga atccaattgc aacaagctgg tcctctcgta agatcagtgg
    75001 ccgcttgatc agcataccat ctgtcgataa gacttggctc gcttttgaag ccgtaaactc
    75061 gcctacttga tctttcagtc caagttcccg atatttcatc ccgcttgtgt tgaacaggcg
    75121 acggattggt acttgactcg tttctagcca agtctcaatc gttgaggcct tcggtacttc
    75181 tttgatcata tcaacagttt gataactaac gccatgctgg tctaaccacg ctttggcttt
    75241 tttgcatgtt gaacatttcg ggtaccagta aaaggtgtac aattttctta cctccttgaa
    75301 gatcttgcag gtgcttcatt ttggtatctc atcgataata tcaacccgat accgatcatg
    75361 ttccctaaaa tcgaagatcc tccctgacta atgaaaggca atgggatccc tgttaaagga
    75421 agtaatccga tatttgctcc aatattctca aagacatgga acagcagcat catgatcaaa
    75481 ccgcttgcga tataggcgta aaattcatta ttcgtatcga aacacacccg aatcatacga
    75541 tagattaaaa taaaatacaa gaaaatgaca aatgcaccac cgataaagcc gaagttttca
    75601 ccgacaacgg taaaaatcat atcggactca cgaacaggca cataaatatt gcttacgttg
    75661 aatcctttac caaacaagcc accggtacca attgccaaaa gtccttgggc cggctgataa
    75721 ctcattcctg acgtatcatg gaaagggtcc agccatgaat caatacgtgc aaactgataa
    75781 gatttgaagc caactttgta aagcaagtct cttccgcctt cagtcgtaac caagaagatc
    75841 gttcctgctc caacgactac tgccaagatt actacaggtg cgatgatgcg ccaagagatt
    75901 ccagacatca agaaaatccc gccaaagatc gcaagaaaga caagcatcgt tccaaagtca
    75961 tcttgggcca tgatcaatgc taaaaccggt attgtcacga tcaagagctt cccaatcagc
    76021 caaaagtccg atttcatcgt ccgttctttt acttgcgtat tatgtttagt tacgatcagt
    76081 gccatcatca agatgtacgc aatcttcatc aactctgctg gctggaaagt aaacgcaccg
    76141 aatcgaaacc agtttttaga ccctgttgaa gcagctaaat tctgatcata gaatttgagc
    76201 aagagtccca ttaccactaa ccccaaacca taaaagtaag gagtcagttt ccagatccat
    76261 ttggaattca aatgcatgat gacaacgatt gctgccgctc caactaaata ccagacaact
    76321 tgcatcccta cgcctttcaa gactgctgga ttgttcggat catggctaag tgctacatat
    76381 aatgagagca gaccaatcaa acataacaga aagactggta aaatcacgcc ataatcaata
    76441 cgattatcgt tcgaagcttt taattttttt tccatgtttc atcccttttc ttaaaaacca
    76501 tactgctgat tattatagcg gtccaattag ataatggaaa gtctttccat agacatttgg
    76561 gaaaaattaa ctttccgttt gtgcagttgc ttgagcaaat gcagtacaaa agcctactgc
    76621 ttatctttag tgtatcatag aacttttttt gatacatgca attatctacc aactgagatt
    76681 ctcctcgctg tgagaagttt ctgtgctttc attgtatcga aggatctctt cggcaatctc
    76741 tagcagctga tcagaaacca gattggttcg ttggatgaaa taaaaataac gatggaaatt
    76801 caacggagtc attggcacat tttctggaac agagcgctta gacaaaatcg agcgaccgat
    76861 tccttccttc agcaacgcaa caattacatc gttacttttc acgatcatct tcttatcttg
    76921 gatattcatc tcttcaaaat aacgctgcgt gtaatgaaaa acaccagagt ttgcttctct
    76981 tacaagccac aatttgcttt ttgggtcccc agcgatgact agctggtctt ccatcaaaga
    77041 gtatctttgt acgccggaag tctctaatgg cttttcgata aaaccaaaat cggattcatg
    77101 tttcgctact tgttctaatg attcatgaga attcgcgatt tcgatctcaa aatgcacttt
    77161 aggaaacctt ttgatcagat gtttcatcaa ctgaggcaaa ggatagacag caaaggtgtg
    77221 ggaagcaacg attcgacata cttcttcttc attttgttca ttgagcaatt tttgctgagt
    77281 ttcttcccat tcttctaaca aaaccagtgt ccgttcatat aataagtcgg cttgcttagt
    77341 tggaagcatc tcttttcttc cgttacgttg aaaaagatcg atattcaatt cccgctccaa
    77401 ctgtttgatt tgattcgaga ctgctggttg agataaaaac agtacttctg ctgctctgga
    77461 aaaatttctt gtttcatata ctgcccgaaa agtcgttaat aacttaaaca tggcaccact
    77521 tccataactt tttatgataa ttactataaa aactatttga tttaaatata caaaaaacca
    77581 tctataatag aaatgtacaa caaaacagtt gctgatccaa ctagtaactt agatgaagac
    77641 aatcagctgt ctgcttgctc gtaatcatac attcctattc attttacaat gtattcgttc
    77701 atcttggtac taaaatctat taaataaaaa ggagaatatt atatatgaag aactttttga
    77761 aaattattcc gatcccgatc tgcgggttga tccttgggtt agcttccctt ggaaatctgt
    77821 ttaaagacta tcaccatgtt gctttaggca atctaactgg cggtatctct atgattctta
    77881 tgatattcat ttttgttaaa ttgtttatct tattcgaaca cacgaaacaa acgttgcatg
    77941 atccaatcat cgcctccgtt tcaccaactt ttacgatgtc actcatggtt atttgtactt
    78001 attttgtttc atatgaaagt attgcaccaa ttgtcaaatt catttggctg tgcgctgtta
    78061 tcttccaagt gatccttgtc ttttatttca attatcacca tgttatcaaa gctgatcttt
    78121 caattgaagc aatttatcca agctggttta tcatttatgt tggatttggg gtcatcactg
    78181 taactgccgg gaacttcttc ccgcttattg gaaaaatttt cttttggatt tcattgatct
    78241 gctatgcgtt cttattgccg atcatcatca aacgtgtctt tttcgtaaga aatatggctc
    78301 atccgacacg tccgctgatt acgatcgttg cagcacctgg ttctctttgc ctaacaggtt
    78361 atcttaaaaa ctttgctgaa cctaattttt atcttgtcgt agcattgttc gctttatctc
    78421 aagtattgta tttcgttgtg ctgtctatgc ttccacgttt gttcaagctt cgtttttatc
    78481 caagctatgc agcctttaca ttcccgcttg ttatctctgc aacagctata tttactactg
    78541 ttcattactt caacagttta ggtatctata gtagcgtatt ggatttctta aaagttgcgg
    78601 aatgtctgtt tgcaagtgta gttgtcttct acgttcttgc tcattacgta cgtttcttaa
    78661 ttaaagaaca tagcaaacat ggaaaatttg tttctaaaaa aatcgcttaa taatcgattc
    78721 aataggttgt gaaaaagctg ttctgcatca agtaataaga acggccaaac aaaaacagct
    78781 gttcatgttt ttgaattggt tgggcttatt accgcagatg caagcttttg aacaccgttt
    78841 accaagaggt cgtaaaagaa gtggccagct tcaagaaata agaaggaatt tacgaaaatt
    78901 gcttctcaaa tttttgtgaa atttcggctt atttccgaaa aagctacttc tggaacaccg
    78961 tttaccaaga ggtcgtgaaa aag
//
LOCUS       DAZUMM010000003        61719 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000003 DAZUMM010000000
VERSION     DAZUMM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.003"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..187)
                     /locus_tag="ACG8E6_000144"
     CDS             complement(59..187)
                     /locus_tag="ACG8E6_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS5060601.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACG8E6_000145"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACG8E6_000145"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS5060602.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="ACG8E6_000146"
     CDS             complement(1674..2729)
                     /locus_tag="ACG8E6_000146"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS5060603.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACG8E6_000147"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACG8E6_000147"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFS5060604.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACG8E6_000148"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACG8E6_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFS5060605.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACG8E6_000149"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACG8E6_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFS5060606.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACG8E6_000150"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACG8E6_000150"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFS5060607.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACG8E6_000151"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACG8E6_000151"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFS5060608.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="ACG8E6_000152"
     CDS             10491..12050
                     /locus_tag="ACG8E6_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFS5060609.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="ACG8E6_000153"
     CDS             complement(12161..14092)
                     /locus_tag="ACG8E6_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS5060610.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACG8E6_000154"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACG8E6_000154"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS5060611.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="ACG8E6_000155"
     CDS             complement(15021..15773)
                     /locus_tag="ACG8E6_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5060612.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="ACG8E6_000156"
     CDS             complement(15932..16141)
                     /locus_tag="ACG8E6_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060613.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="ACG8E6_000157"
     CDS             complement(16152..17480)
                     /locus_tag="ACG8E6_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060614.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="ACG8E6_000158"
     CDS             17616..17771
                     /locus_tag="ACG8E6_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFS5060615.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACG8E6_000159"
     CDS             complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACG8E6_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFS5060616.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACG8E6_000160"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACG8E6_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFS5060617.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACG8E6_000161"
     CDS             complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACG8E6_000161"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFS5060618.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="ACG8E6_000162"
     CDS             complement(21455..22387)
                     /locus_tag="ACG8E6_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060619.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="ACG8E6_000163"
     CDS             complement(22544..23203)
                     /locus_tag="ACG8E6_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS5060620.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACG8E6_000164"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACG8E6_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS5060621.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="ACG8E6_000165"
     CDS             complement(24244..24534)
                     /locus_tag="ACG8E6_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060622.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="ACG8E6_000166"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="ACG8E6_000166"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFS5060623.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /gene="glmM"
                     /locus_tag="ACG8E6_000167"
     CDS             complement(26821..28176)
                     /gene="glmM"
                     /locus_tag="ACG8E6_000167"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFS5060624.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="ACG8E6_000168"
     CDS             complement(28200..29369)
                     /locus_tag="ACG8E6_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFS5060625.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /gene="cdaA"
                     /locus_tag="ACG8E6_000169"
     CDS             complement(29366..30253)
                     /gene="cdaA"
                     /locus_tag="ACG8E6_000169"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFS5060626.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="ACG8E6_000170"
     CDS             complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="ACG8E6_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFS5060627.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34320..34841
                     /locus_tag="ACG8E6_000171"
     CDS             34320..34841
                     /locus_tag="ACG8E6_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFS5060628.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35540..36105)
                     /locus_tag="ACG8E6_000172"
                     /pseudo
     CDS             complement(35540..36105)
                     /locus_tag="ACG8E6_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36284..36940)
                     /locus_tag="ACG8E6_000173"
     CDS             complement(36284..36940)
                     /locus_tag="ACG8E6_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060629.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="ACG8E6_000174"
     CDS             complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="ACG8E6_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFS5060630.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38477..39475)
                     /gene="yvcK"
                     /locus_tag="ACG8E6_000175"
     CDS             complement(38477..39475)
                     /gene="yvcK"
                     /locus_tag="ACG8E6_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFS5060631.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="ACG8E6_000176"
     CDS             complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="ACG8E6_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFS5060632.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40491..41222)
                     /locus_tag="ACG8E6_000177"
     CDS             complement(40491..41222)
                     /locus_tag="ACG8E6_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFS5060633.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41226..42491)
                     /locus_tag="ACG8E6_000178"
     CDS             complement(41226..42491)
                     /locus_tag="ACG8E6_000178"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFS5060634.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="ACG8E6_000179"
     CDS             complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="ACG8E6_000179"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFS5060635.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="ACG8E6_000180"
     CDS             complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="ACG8E6_000180"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFS5060636.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47833..49335
                     /locus_tag="ACG8E6_000181"
     CDS             47833..49335
                     /locus_tag="ACG8E6_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFS5060637.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49337..50074
                     /locus_tag="ACG8E6_000182"
     CDS             49337..50074
                     /locus_tag="ACG8E6_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5060638.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="ACG8E6_000183"
     CDS             complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="ACG8E6_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFS5060639.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="ACG8E6_000184"
     CDS             complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="ACG8E6_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFS5060640.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="ACG8E6_000185"
     CDS             complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="ACG8E6_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFS5060641.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="ACG8E6_000186"
     CDS             complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="ACG8E6_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFS5060642.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(55574..56740)
                     /gene="hemW"
                     /locus_tag="ACG8E6_000187"
     CDS             complement(55574..56740)
                     /gene="hemW"
                     /locus_tag="ACG8E6_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFS5060643.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(56756..56983)
                     /locus_tag="ACG8E6_000188"
     CDS             complement(56756..56983)
                     /locus_tag="ACG8E6_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFS5060644.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(57303..57626)
                     /gene="relB"
                     /locus_tag="ACG8E6_000189"
     CDS             complement(57303..57626)
                     /gene="relB"
                     /locus_tag="ACG8E6_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFS5060645.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            complement(57969..58415)
                     /locus_tag="ACG8E6_000190"
     CDS             complement(57969..58415)
                     /locus_tag="ACG8E6_000190"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS5060646.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            complement(58396..58992)
                     /locus_tag="ACG8E6_000191"
     CDS             complement(58396..58992)
                     /locus_tag="ACG8E6_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFS5060647.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            complement(59476..59637)
                     /locus_tag="ACG8E6_000192"
     CDS             complement(59476..59637)
                     /locus_tag="ACG8E6_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060648.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
     gene            complement(59630..60304)
                     /locus_tag="ACG8E6_000193"
                     /pseudo
     CDS             complement(59630..60304)
                     /locus_tag="ACG8E6_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            60827..61434
                     /locus_tag="ACG8E6_000194"
                     /pseudo
     CDS             60827..61434
                     /locus_tag="ACG8E6_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
BASE COUNT        17123 a        13253 c        10688 g        20655 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
    27541 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
    29221 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
    29461 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
    29521 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
    29761 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg tcgttttttc
    30541 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
    30601 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cttggatagt
    30661 cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg aaaagcgaac
    30721 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
    30781 tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc aatagccaga
    30841 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca aaccatgtgt
    30901 gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg cttcttcaaa
    30961 agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
    31021 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
    31081 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
    31141 ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat gatcgatccc
    31201 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
    31261 gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc gttcgctttc
    31321 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa acatgtgtac
    31381 caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
    31441 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
    31501 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
    31561 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
    31621 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
    31681 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
    31741 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
    31801 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
    31861 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
    31921 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
    31981 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
    32041 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
    32101 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
    32161 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
    32221 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
    32281 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat
    32341 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
    32401 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
    32461 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
    32521 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag
    32581 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
    32641 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
    32701 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
    32761 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
    32821 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
    32881 ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat catccacgat
    32941 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
    33001 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
    33061 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
    33121 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
    33181 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
    33241 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
    33301 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
    33361 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
    33421 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
    33481 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
    33541 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
    33601 acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag caagatgggc
    33661 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
    33721 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
    33781 tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
    33841 cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg caccagtttt
    33901 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
    33961 tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta attcagccat
    34021 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
    34081 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
    34141 atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt tcttttattt
    34201 ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga caaacagtct
    34261 aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg tgttttaaca
    34321 tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc ctttatggtt
    34381 catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg tattgccgac
    34441 aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg caattgcagc
    34501 gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac gtgatgcacc
    34561 ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa atattcgata
    34621 ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca gtagataaac
    34681 taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac gaagctgagc
    34741 aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt gatgaatcta
    34801 tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag gagcgtagga
    34861 caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg gaagcagaag
    34921 caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa
    34981 atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa gctgagcaaa
    35041 ttatctccac caaataattt attcatattt ttgacttgcg catactaact aattcttaaa
    35101 ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg gtgattcaca
    35161 ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc
    35221 ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg tttattttaa
    35281 acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa
    35341 atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact cctaactcct
    35401 ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca cagcctagta
    35461 tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa
    35521 atggattaat agatttcgat tattcaaagc gattttctat tggtggattg tgttcccaac
    35581 taacaagacg gattccaaag tcattatata actcttcaag agttaaccct ttcctgttcc
    35641 caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg ccaagacctg
    35701 gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa atccagctcc
    35761 tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata attgttgtgc
    35821 cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc tacctgcatc
    35881 tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc
    35941 tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc ttggtggctt
    36001 aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac
    36061 aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc gcaaatcacc
    36121 tctacattct cacatactag ttcgtaatac tcctttaata tcaaaatatc tactgatcgt
    36181 aataatattc gctatgaatt cctactactc gtataagaaa ggccaaatca tcagctttta
    36241 cttgctgatg atttggcttg tttctattta tcgtaattgt tttctaatca ccaaaattgt
    36301 taacaatcgg ttcagatatt tcccaattca ctagccttat attatattga tttaacaatt
    36361 cttcttcatt tattgtattt tgatcaagcg gaccaaattg ttctaataat tctttgtcat
    36421 gtaaaatatc ccttctagta atgcctcttt ccctttcaat gaaagaagca ctaggaaaag
    36481 tatgattata accattcaac ctatctccca tgtaataatt agcaggtcct ggaaaataaa
    36541 atatttgctt attatcacta acttctaaca gcaacactaa atcttctccg ctggctaaac
    36601 gacttgtcca tctacgatat ctccctatgc cttcgttcat tccaaatcca tcaaattcaa
    36661 ctattaacgt gtcttctatc acatgtaatt cttcattcaa ttcatactca agtctaaaag
    36721 gaaattctcc atatgtaatc tctggcaatg gtggtgctgg agttctccaa ataaatacaa
    36781 taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct gctcgaagta
    36841 aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa ataaaaagta
    36901 ctgcaatcat aatgatagta aaagttgtaa aaaaacgcat ttttgccctc cctttataaa
    36961 catcgattct caaattctaa accaaatctt ggtagttcaa gttcaggtat aacgtattcc
    37021 aaccatacat ctcaattact atttatgaac ttatgattat cactgaataa atcactccat
    37081 ccccttgtta aaacttctac attaattaca cctggttgtt ttcgactagc taatccaatg
    37141 aacttatcaa ccatccttat ccaccaacca cgagaatctt gaccagaagt aagaagagca
    37201 gttctcaagg atgatcgagc agttccttcc agttttttaa ttaatcaggc cagttataga
    37261 taaatacttg atcttatcta tatcaggact tctatattaa gcaaaaattt atgtacaaaa
    37321 ctaaaatctt cattcagttg tctccccgct ttttatacga ttgtattaaa aacaatagaa
    37381 gcctttccaa attatgttac tccagtaaag atggaattat agtttgacgg aagaaaagca
    37441 agtataactt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta tcagtttttt
    37501 ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt ttgcgaattc
    37561 ggtgattaat tcccgatttt gatatcgcac ctgagggaat catttcccct aattctttca
    37621 agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt tcaggaaggg
    37681 catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta gaagccgcat
    37741 caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc gaatttctca
    37801 tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca ccaatcaatg
    37861 ttaagaaatc agcaattttt tctgctcctt ttaaataaga aatatagcca ttgcgccgtt
    37921 caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct tggttatgct
    37981 gttcataaat agaaaatatt tccaaatgat aacggctcgt ttccggatta ttgactgaac
    38041 ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt cccataatct
    38101 cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc atcaacacat
    38161 cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat ttcatttttt
    38221 tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa tagatacgtc
    38281 tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc acaataccta
    38341 aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct cgatgaactt
    38401 ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc tcctcctttc
    38461 tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat tcttctacta
    38521 ctttgtctcc atcgtggaag acaccaccat ctttcaattc caaaaagtcg gtggaaatca
    38581 ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact agatactcat
    38641 catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc aatactgtat
    38701 cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca tctgtgaaat
    38761 gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca gcagttgtct
    38821 tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg gtgaataaac
    38881 ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact tttcgagccg
    38941 ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg gttttacggt
    39001 cgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaagaca gcatgcaagg
    39061 tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt tttgctaata
    39121 attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg atcagattac
    39181 cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg aaaatatctt
    39241 catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca ccaggaggag
    39301 ccatagcgat cgattctctg attgcaccgc tgcttccccc atcatctgca acagtcacta
    39361 ctgctgtgat atctgctcct tgatttcgca aacttttcag gataactggc agaccagtgc
    39421 caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt ttcacgaccg
    39481 gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt tatagttttt
    39541 ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac cgcctgtaca
    39601 gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca atacagtttc
    39661 cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca tcacataatc
    39721 atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat gagggttggg
    39781 caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt taaaaccaaa
    39841 tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact cattgatttt
    39901 ttcacgtaat tgtcgtggtg tcaatgtcgt cgtatcaatg atcacggaag cttgggcttt
    39961 caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac cgtccatcgc
    40021 cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt ctgaagcatc
    40081 taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca gcatgttttg
    40141 gatttcttca aagaaggaac gagaacgtaa atccactcct aaagccattt ttgtgacttt
    40201 gcctgattct ttgattaatt cccagaattt tggaatcaat cgaggcggca tattatcgat
    40261 acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg caccgctcat
    40321 acctgttatg atgactaatt gcaaattatc tgtcataatt acgaactcct ctcacattgt
    40381 gcatagtaaa attatatcat tccgggcgta tcattaccag ttttcttttt cttttacaaa
    40441 aagatttgga ccaaatatct tttaggatat ttgccccaaa tccaattttt ttacttttcc
    40501 gatgctgtat ccaatgcctt gcttctttca gctagcgctc gttcaatcgt ccgatctgct
    40561 aaaatagatt gggcatcgat cactggataa tggttatctt cagcaaattc atgcatcaat
    40621 gacaattctg tgcaacctaa gataactttt tcacagttta ggcgctctac cgcttcttct
    40681 aagatttcgt aatatagctc ttgattcaaa taatccgatt ccttgatctc atgatagatc
    40741 agataattga ttttttcttg aagaacagta tcaggaatca ctgtttcgaa tccaagattt
    40801 ttgacttcgc gttcatagat tccagctttc atacttcctt ctgttcctag aatcgctact
    40861 ctccctgtcg tatgctgacg gacaagttca tttgctgctt ctcttggcat atgaaggata
    40921 ggaatatccg ttgccgcttg taattcttca aaaaagtagt gtgcggtgtt acaagtcaac
    40981 acaatgaagt tcggctgcaa taaattttgt ttctcaatat catccagcag aaacggcatt
    41041 ggattttctt ctgatcgatc taaaatataa gctgtacgat caggaactgt tgcatgattg
    41101 aataagacat aattcaaata ttcttgatct tttgtagctt tcgttcggtg gttgatcaag
    41161 cgaacaaaac tttctgtcgc catcgtgccc attccgccta aaatactgaa aaaattctcc
    41221 atgaactagc cttctttcac atgaaaatac tttttgaatc ttggaatata gttatggaac
    41281 atatatttca tcaacaacca gcgcttgatt gaccggtctt tttcataaaa cacagtagta
    41341 ccgtaacgat tttctttgat catttgctca gctagttttt tgtcttcatt ttcccgagca
    41401 tattctaaaa agattttttt gggcacaccc atccataatc ttgctttatc tgattgattc
    41461 gttccatagg tcgtttcaac aaagggtttg ttaaacactc gatcttctgt gacgaaacga
    41521 gccagattca agccgttcaa tgtcacaaag aaactgctac gaccttgtct caaattgatt
    41581 tcaaataatt tatattctcc atctctagga tcatacttca tatcgaaatt ggcaaagcct
    41641 gtatattcga ttttttccaa aaatgcctta atggtttgat agattttttc gttataatct
    41701 ggcatgatca caacgtaatt accaatcgat gctggtgtag gatcttctaa cagtggatga
    41761 ccaagacaca tcatccgaac ttgatgatct tcatctacat acgcatttag cacacgcata
    41821 ttactatcat cacccgggat aaaatcttgt acaatcattt cacttttata accggcttcg
    41881 tatatccggc ctaaaattaa atcgaactct tctctagtct ctaaaataaa agcttttttt
    41941 cttccttcaa attggacaga aagatactcc acactgtttg caggtttcaa agcaactggg
    42001 aattcaaaag gcaattcctg ttccaaatgt ccatttacta acatttcttc tctgacgatc
    42061 aatgttttag gatacggaag atcatattct tcacaaactt cataaaagct tactttattg
    42121 atcagtcttt caaataaaga ataatcaata tatggacaga taaaatattc tgacagctct
    42181 tgtttatgtt gggagatcaa ttctgcatat ccatcaccac aagcaatcaa aagatatttt
    42241 ttactcttat cagaatatct ttcttttgcc aaacgaagca tcgtctcgat aaacacaggg
    42301 tctttatcaa atcctggaat aacttctaca ttgacaattt tactgtagcg ggtaggggct
    42361 aactgatccg aagcatatgc ttggcagatt ttgccgtatg cttcattaaa agaacgtgcc
    42421 ataccataaa cgttcatgtc gcttcctaat aatataggga taaattcttc attttcaata
    42481 ctgttcatca ttatctctca cttactcata atctcttgtt tttcttttcc aactacacaa
    42541 ggaatatcat accataaaga aaaaagcaat caaaggaaac tcctccgatt gctcaattct
    42601 tttttgaacg gattgaactt ataggaagtt tttgtctata ccaatcagtg tagaaagaac
    42661 taccatgtaa tgattctaga acactgatat taataaaaca agcaaaaatt cattttttta
    42721 tgttttttta actatctcag ctatctatcc tctttatacc attatttttt tcaaatagtg
    42781 acctgtataa ctttctttta cgttaatgat ttcttctggt gttccagtcg caaggatcgt
    42841 tcctccacct tctccacctt ctggtcctaa atcaatgata taatctgctg ttttgattac
    42901 atctagattg tgttcaatca ctaaaactgt gttaccagca tctactaatc tttgtaatac
    42961 atgcaacaag cgggcgatgt catcgctatg aagtcctgtc gttggttcat ctagtatata
    43021 gaaattcttt ccattagaga ttttgtgcaa ttcactggca agtttcatcc gttgtgcctc
    43081 accaccggac aatgtcgttg ctggttgccc catcgtcaca taacctaagc caacatcaac
    43141 aatcgtttgc agtttgcgat gaatctttgg aatgtgcttg aagaattcta cagcatcttc
    43201 tactgtcatt tccaaaatat cagcaatgct ttttcctttg taatgcactt ctaatgtttc
    43261 agagttatat cgtttgccat gacatacttc acaaggaaca tagacatcag gcaaaaagtg
    43321 catttcgatc ttaataattc catccccgcg acaagcttca caacgaccgc cttttacgtt
    43381 gaaactaaaa cgccctttct tataaccccg catttttgcc tcgttcgttt gagcaaataa
    43441 cccacggata tcatcaaata cacttgtata agtcgctgga ttacttctcg gcgtccggcc
    43501 gattgggctt tgatcgatat cgatgatctt ttcgatactt tcgtagccgg aaattgtctt
    43561 gaatttacct ggcttagcag aattcttatt taatttttgc gctaacgatt tctttaagat
    43621 actattgacc aatgtactct ttccagaacc agacacacct gtgactgcta caaattctcc
    43681 taatggaaat tcaacactaa tatttttcag attgttttct gaagcaccag tgatcttgat
    43741 tgctttccca ttacctttac ggcgttcttt tggtacaggg attgattttt tccctgaaag
    43801 atatttccct gtcaaagaat tttcatcatt agctacttct tcaggagtgc ctgcagccac
    43861 gatttgtccg ccttgttctc cggccccagg tccgacgtca atcagataat ctgctgccca
    43921 catcgtatct tcatcatgtt cgactacaac gagggtgttg ccaagatcac gcattttctt
    43981 taatgagcca atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg gttcatctaa
    44041 aatatacaaa actccagaaa gattcgatcc aatttgtgta gccaatctga ttcgctgcgc
    44101 ttctccccca gataatgttc cggaagcgcg actcaaagtt agatagtcta gtcccacatt
    44161 ttccaagaat gaaagtctgt catttacttc ttttaaaatc ggtttggcaa ttactttctc
    44221 ttgttcagat aacgttaagt ttgagaagaa atctactgct ttttgaatag acaactcatt
    44281 tgtttgcccg atattcgttc cattgatttg aacagataga gcttggggat tcaatcgata
    44341 acctttacat gtctgacaag ttaattctgt catataaaga cgcatttgct cacgcgtgaa
    44401 atcactgttc gtatcatgat aacgacgctc gatattcttc aatacccctt cgaaagtcgt
    44461 ttctacatcg cgaacgccac caaaatcatt ttcataatgg aaatggaaaa gatcatctgt
    44521 cccattcaag atgatatcct gatgctcttc cggtaattct tcgaatggtg tatctaaatc
    44581 gataccgaaa ctttcacaag cctgcgctaa catttgagga taatactgag agctgattgg
    44641 attccatgga acgatagcac cttgactaag tgtcttcgtt ctatctggaa tcactaaatc
    44701 ttgatcgact tctaatttga cacctaaacc atcacagtct ggacaagctc caaaaggcgc
    44761 attgaatgag aacagtcttg gttctagttc gccaacagta aatccgcaat aaggacaggc
    44821 atagtgttcg ctaaacaaca tttcttcttc accgatcacg tcaaccagag catagccgtc
    44881 tgccaatcgc agagccgcct ctaaagaatc aaatagacga gaacgaatcc cttctttcac
    44941 aatgatccgg tcgatgacga tcgcgatatc atgttttttg tttttctcca gctcaggcac
    45001 ttcacttaaa tcatagatct caccatcgac tcgaacgcgg acgtatccct ctttttggat
    45061 tcgttcgaat atttttttat gttgtccttt tttctttacc acgatcggtg ccaacacttg
    45121 gatctttgtg cgctcaggca aagccattac ttgatctgcc atttgatcta ctgattgact
    45181 ggtaatctcg atatgatcgt tgggacaaat cggatgtcct acacgtgcaa acaacaagcg
    45241 taaatagtca ttgatttccg tcacagtacc tactgtcgac cgaggatttt tacttgttgt
    45301 tttctgatca atagaaatcg ccggactcaa tccgtcgatg ctatctacgt ccggcttatc
    45361 catttgtcct aaaaactgac gagcataagc agaaaggctc tctacgtatc tccgctgccc
    45421 ttcagcgtac agcgtatcaa atgctaggga actttttcca gaaccggaca aacctgtaac
    45481 gaccacgaat ttatctcgtg gaatcgttac atctatattt ttcaagttat gggcacgtgc
    45541 accgtgaatc gtaattttat cgttagccat gttgtcctcc tactttgaag cttttaattc
    45601 taaaatcata tctcgcacat tagcggctgt ttcaaaatcc aatgctttcg ccgcattttt
    45661 catttctttt tctaatttca tcaataacgt atctttttct tctttcgtca tttcttcata
    45721 cgagacttgt accgcttctt ttgtatcatc ttccgattct ttcgtaatag aaatcaaatc
    45781 tcggatttct ttaatgatcg tttttggaac gatcccatgt tcttcattgt acttctgctg
    45841 aatcgtacgg cgtcttgatg tttcatccat cgctagacgc atagaatctg ttactttgtc
    45901 tgcgtacatg attactttac cttcttcatt ccgagcagct cgtccaatcg tttggactaa
    45961 ggaacgttca ctacgtaaaa agccttcttt atccgcatcc aagatagcaa caagggaaac
    46021 ttcaggtacg tctaatcctt cacgaagcaa attgattcca actaacacat caaattcacc
    46081 taatcgaaga tctcgaatga tttctgttcg ttccagcgtt ttgatatcac tatgcaaata
    46141 tttgactttg atccctaatt ctttgaaata atctgtgagg tcttctgcca tttttttcgt
    46201 caatgtcgtt acaaacaccc gctggtcttt ttcgactcgt tcatttattt cgcctactag
    46261 atcatcgatt tgtcccatga ttggacgaac ttcgatcact gggtcaagca gccctgtcgg
    46321 acggatgatt tgttggataa cggtatctgt ttgttcatgt tcatatggtc ctggcgtcgc
    46381 agagacataa atgatctgat tgacatgttt ttcaaactct tctaaccgaa gtggccgatt
    46441 gtctaaagca gatggcaaac ggaaaccgta atctactagc atctgttttc tcgctcggtc
    46501 accattatac attcctctga cttgaggcat ggttacgtgc gattcgtccg ccacgatcaa
    46561 aaaatcttca ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt tacgtccgtc
    46621 catatgccga gaataatttt caattcccga cgtgtagccc atttctaaca tcatttcaat
    46681 atcgtaattg gttcgctgtt ctaaacgttg agcttccaga agtttgtttt catttcgcaa
    46741 gacagttaaa cgctgttcta attccgcttt gatattcgct acggcatgct ccatatgttc
    46801 atcattcgtc acaaagtgag tcgccgggaa gattgccacg tgttccgttt ctcctaaaac
    46861 ttcacctgtc agtgcattga cttctcggat acgttcgacc tcatctccaa aaaactccac
    46921 acgtaaggca cgttcatctc ttgaagccgg aaaaatctcc acgacatcac cgcgcactcg
    46981 aaaacgaccg cgctgaaaat ctatatcatt tctttcgaat tggatactga ctaagtcacg
    47041 aatcaactga ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt gtttttggta
    47101 ctcaaaaggc gaacctaatc caaaaataca tgaaacggat gcaatgacga tcacatcatt
    47161 tctttctaat aatgaactag tagccgaatg tcgtaatttg tcgatctcat catttacact
    47221 tgaatctttt tcgatatacg tatcgcttga aggcacataa gcttcaggct gataataatc
    47281 ataataagaa acaaaatatt caacagcatt gttagggaaa aattccttga attccccgta
    47341 cagctgacca gccaatgttt tgttatgggc aataattagt gttggtttat tcactttctc
    47401 aataagattg gaaatcgtat atgttttccc tgttccagtc gcacctaata agatttgtgc
    47461 ttttttcccg ccgactacgc catccactaa ttggttaatc gcttcaggct gatcaccagc
    47521 cggttggtat ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa tcattcttcg
    47581 tcctcctttt cattcattcc ttttcatttt acgaccaata aaaagaaaag gaaagcaaag
    47641 gcattcctta ctaaaaaatt ataccacacc gaacatactt tcgccacttt tttatcttat
    47701 tatgcatgtt acgttttata acataaaatg ttagaaactc gcattttttt cattttttcg
    47761 agcttacagt cttgtctgat tcagcacttt tcgcctataa taataaaata ttggaaattt
    47821 aggaggttga ttatgaaaag aaagcattta tttcactcat ttgttttctt aatgacagga
    47881 cttgtgacac ttgcgtttgg acatgttgca caagctgaca acactacaac ggcagagtca
    47941 agcacaagcg agacaatgca aaaaatagag cccaaaaaag acgtatacgt aattgccagt
    48001 gactctgctt ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga aggcatcgat
    48061 gtaaaattga tggaaaacat cgccaaactg caaggattta acatccgaat ggactaccgt
    48121 ggtttctctg ctgctttgca agctttggaa tcaggacaag cagacggaat gattgccgga
    48181 atgactgtaa cagatgaacg aaagaaaagc tatgattttt ctgattctta ctttgatagc
    48241 gggatccaat tagctgtcaa aaaaggagat acttcgatca agtcttatga agatttgaaa
    48301 ggaaaaacag tcggagcaaa agttggtacc gaaagtgctg aatttttaag cgaccatgaa
    48361 tctgaatatg gctattctat caaatatttt gatgctgctg atcaattgta cgacgggtta
    48421 aaagttggcg caatcgatgc aatgatggat gattatccgg taatcggtta tgcgatcaaa
    48481 caaggacaag aattggaaac accgatcaaa aaagaatctg gcggaaaata tgcatttgcc
    48541 gttaaaaaag gacaaaatcc tgagcttctt aatatgttca atgaaggatt gaaagaatta
    48601 aaaagaacag gcgaatacga taagattatt aaagaatatg tagcggatgg ttcagaaact
    48661 tccagtgagt cttccactgc tgatgaatca acatttcttg gacttatcca aaacaactat
    48721 caagcattgt tgaaaggact ttggagaaca attctcctaa ctttgatctc ctttgctttg
    48781 gctatagtaa tcggtgtgat ttttggatta ttcagtgtag caccaatacg cgctcttcgc
    48841 acaattgcta gtatttatgt cgatatcatc cgaggaattc caatgatggt tcttgcgttc
    48901 tttattttct ttgggttgcc tggtatcgtt ggatttacga ttcctgactt ccttgctggt
    48961 gttatcacct tgactcttaa cgcaagtgct tatatcgcag agattgttcg aggcgggatc
    49021 aacgccgttc ctgtcggaca aatggaagct tccagaagtt taggtctttc ttataatcgg
    49081 acaatgcaaa aaatcatttt gccgcaagcg atacgcatca tgatcccatc attcgtcaat
    49141 cagttcgtta tctcattaaa agacacaacg atcatttccg caatcggtgt agtcgaattg
    49201 ctgcaaactg gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt ttacttgatc
    49261 attgcgatca tgtacttgat tttgatcaca gcattgacaa aattagcaaa agttttagaa
    49321 aagaaggtga agtaagatgg cagaaaaaat cttagttgag catttagtca aaaaatacgg
    49381 cgataacaca gtattaaacg acatcaatgt gtccatcaac gaaggagatg tcgtctgtat
    49441 cattggccct tcaggttcag gaaaaagtac atttttgcgt tgcttgaatc gtttagaaga
    49501 accttctgac ggtgaaatta ttattgacgg cgcccacttg atggataaaa gcacagatat
    49561 caacttggtg agacaacata tcggaatggt attccagcac tttaacctat tccctcatct
    49621 ttctattttg gaaaacatca ttttagcacc gatggacctg aagaaagaat ctagagaaga
    49681 agcagagaaa aaagcaatca aattgctgca gacagttggt ctagaagata aaaaagatat
    49741 gtatcctgaa atgctttccg gtggacaaaa gcagcgtgtg gctattgctc gtgctctagc
    49801 gatgaaccct gacatcatgc tttttgatga gcctacatct gcactcgacc ccgaaatggt
    49861 cggtgatgtc ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga tggttatcgt
    49921 tacgcacgaa atgggcttcg ctaaagaagt ggccaatcgt gtcatgttca ttgacgacgg
    49981 aaacttttta gaagacggaa cgcctcaaca aatctttgaa aatccgcaaa atgaacggac
    50041 aaaagatttc ttgaataaag tattaaatat ttaataaaaa ccaaaaatgc ccggtcgctt
    50101 catgacgacc gggcattttt agtttcttgt tttttcactt aactgtacca gccgcatcgc
    50161 ttgttctcct gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat
    50221 cggttcattg acgatatcaa gtacatagtc gataccacct tcagaaacta tgatgattat
    50281 cttattctaa agctggcttt tttgttggta caaacatgac aactacaaaa aaaccaggcg
    50341 aacgtatgat tgttcgtctg gtttttaatt attaatgact tagcgttttt actagaaata
    50401 gaaaatttgg gcaagtctac tttatatttt tactagaaaa actacttccg ctttttcccg
    50461 ccaaaagcat ctttaaactt atcgaagaaa ccgtcttctt gctgttcttc aactttgatg
    50521 ccgccagctt cagcgaatgc acgtaatgct tctctttgtt gttcatttag atttttcggt
    50581 gtgatcagtt ttactttgac atgttggtcg cctgttgcac caccacgtaa cttcggagcg
    50641 ccttttccac gtaaacggaa gttcgtacct gtctgtgttc cagcagggat cttcaatttc
    50701 acatctccat ggacagttgg tacttggact tcatccccca aagctgcttg tacaaaactt
    50761 aaaggcaatt catagtaaat ttcagaacct tcacgatcaa agatatcact ttcttctaca
    50821 cgaaaaacca catacaaatc accaaatggt ccgccgtttt ctccagcttc tccttgacca
    50881 gctaaacgca tttgttgtcc ttcttctaca ccagctggta cattcacttt aactgaatgt
    50941 gctttcttct catgtcctga gccatgacag gttggacaag gttctttgat ttctttccct
    51001 gtgccatgac aaacatcaca agtttgacgg ctcatcactc gtccaagtgg cgtttggcgt
    51061 tctacattga tcgtacctgc accatgacat ttatgacaag tagttggttg tgtgcctggt
    51121 tttgcaccgt ttccaccaca agtatgacag atttcttccc gattatactt gatttctttt
    51181 tctacaccaa aaatcgcttc ttcaaaagac aagtcgaccg tatattgcaa atcagcacct
    51241 tgacgaggcg catttggatc gactgaccga ccgcctcctc caaagaaaga gtcgaagata
    51301 tcttcaaaac caccgaagcc gccaccagag aagccgccga atccaccaaa tccgccacca
    51361 gctccaccag caccgtaatt tggatctgtg cctgcatgcc cgtattggtc gtatgcagcg
    51421 cgtttttgcg ggtcacttaa aatctcatat gcttctgata cttctttgaa tttttcttcc
    51481 gcatcaggtt ctttattgat gtcaggatgg tattttttcg aaagcttacg ataagctttc
    51541 tttatttcat cttctgatgc tccttttgag agccctaaga cttcataata atcacgtttt
    51601 gttgccatag gctttcctcc tattggttct catcattttc acgtcgcttg ttatcatatc
    51661 atattttgtt tgaaacaaaa accgatactt caaactattt ttcaaaagaa aaagccaaag
    51721 tagcaacttt ggccttctct cgaaattcag tgaactgatt atttatcgtc gccatcgact
    51781 tcttcaaaat cagcatcaac gacatcatct gctccgcctt gagctgcttc tggattttct
    51841 tgagcttgtt gttgtgcagc ttgttcgtat agtttaacag tcaagttttg aacgatttcg
    51901 ttcaatgcat cgcgtttagt tttcatttcc tcgatgttgt ttgcttcaac agcggctttt
    51961 aattcgtcac gagctgtttc tgcttttttc acttcttctt cgtccacttt gccttctaat
    52021 tctttcaatg ttttgtccac agagaatagc aatgcgtcta catcattacg taggtcaact
    52081 tcctctttac gagctttatc tgcttcagcg tttgcttctg catctttcac catacgttcg
    52141 atttcttcgt ctgttagacc tgaagaagat ttgatcgtga ttttttgttc tttttgtgta
    52201 cctagatctt tcgcagatac atttacaatc ccgtttttat caatatcgaa tgttacttcg
    52261 atttgtggaa taccacgagg agctgctgga atatctgtca attggaatct tcctagagtt
    52321 ttgttgtcag cagccattgg gcgttcacct tgtaatacat ggatgtctac tgctggttga
    52381 ttatctgccg cagttgagaa tacttgtgat ttacttgttg gaatcgttgt attgcggtca
    52441 atcaatttag tgaacactcc acccattgtt tcgataccta atgacaatgg tgtaacgtca
    52501 agtaaaacaa catctttcac atcaccagtg atcacgccac cttggatagc agcacccata
    52561 gcaactactt catcagggtt aactgatttg tttggttctt ttcctgtttc tttacgtact
    52621 gcttctacaa ctgcagggat acgagtagat ccaccgacta aaataacttc gtcgatttct
    52681 gattgtgaca gacctgcatc ttttaacgct tgacgtactg gtacttttgt acgttctact
    52741 aagtcagctg ttaattcatc aaattttgca cgtgtcaatg tcatttccaa atgcaatggt
    52801 ccagattctc ctgctgtgat aaatggcaag ctgatttgtg tgcttgatac gccagataaa
    52861 tcttttttcg ctttttcagc agcgtctttc aagcgttgta aagccatttt gtcttgagac
    52921 aagtcaatgc tgttttcttt tttgaattct gctaccatgt agtcgatgat tttgttatca
    52981 aagtcgtccc cacctagatg gttgtcgcca gctgttgaca atacatcaaa gacaccgtct
    53041 cctagttcaa ggatagatac gtcaaaagta ccgccaccaa ggtcgaatac taagattttt
    53101 tcgtcgcgat ctgttttatc taaaccatag gcaagagcag ctgcagttgg ttcattgacg
    53161 atacgttcta cttctagacc agcgatctta ccagcatctt ttgttgcttg acgttgtgca
    53221 tcattaaagt aagctggcac agtgataact gctttttcga ctttttcacc taaatagtct
    53281 tctgcgaatc ctttgatata ttgaaggatc attgctgaga tttcttgtgg tgtatatgat
    53341 ttaccttcta catctacttt gtaacctgct tcaccgatat ggcgtttgat tgatgaaatc
    53401 gtatttgggt ttgtcacagc ttgacgtttt gcaacttccc ctacttggat ttctccattt
    53461 ttgaatgata caacagaagg tgttgtacgg tttccttctg ggttagcgat gatttttgct
    53521 tcgtttcctt ccaatactgc tactgcagag ttagtcgtac ctaagtcaat accgataatt
    53581 ttactcataa ttaacgtctc cttctttaat tgaaagtgta agttttatat ccgcacaatt
    53641 attgtgcaac aatgaccata gttggtctta acacgcgatc atgtaatttg tagccttctt
    53701 gtagtacttc tacaatcgta tcggctggtg tatcttcagt cgctggaaca gtttgtactg
    53761 cttgatgaag attaggatca aaggcttctc ctttagcagg gattttttca attccttctt
    53821 cttccagtgc gttacgcaaa ctttccaaga ccatttccac gccttttttc aaacctgccc
    53881 cttgatcatc tgacacttct gtggctaatg cacgttccag gttgtcaatt gatggaagta
    53941 attttttggc taaatcttgt gaacggtatt tttgtaattg ctcacgctca ttttttcctc
    54001 gatttgccat atttgcgatt tcagcacgag cccgcaagta tttatcttcc atttcttcta
    54061 actcagcttt taatttcgct gtttcgaatt cttccgcttc agcttctgaa atacccgaat
    54121 cttctgcagc ttcgacatcg atttccggtt ctggctgagc atctgtcatt tctttatcta
    54181 gttcttcttg attttctttc acgatacagt cttcctttca ttattcagtc agctgtttga
    54241 ttgctcttac taagaaagag agaacgatca gctgcaaata gcttcgttat tcacattacg
    54301 agtttgacaa gtcaagcgag cggtagtaat cagcaagttt agctgctagt tctcttcgat
    54361 aaacatcgac tagaccgaac atccgagaat aaggcatact agtaggccct aacaaggcta
    54421 ttgtacctct tccatgacca tctatctcgt aacttgcttg aatcatactc atattctgca
    54481 ataaatcatt gcctaattca gcaccaatct tgatatgaat attgctatcc atcggagtga
    54541 tcatttgtgt aagtccgtct gagtctttca taaaagaaaa catcgatttg aactgatcaa
    54601 gatcttgacg gggctcataa tccaagagat tcatttgtcc actgacataa accttttctt
    54661 caaaaacatg accaagaacg gtgtcaaata actgagacat cccttctgtc gtctggaaat
    54721 atttatgaag aatcatcgga atctccgtac gaagtttatg gtacaccgtc atcagaggat
    54781 ctccgactag acggtcgttt acaatctgta ccattttttc tagttcctga cttcccatat
    54841 attcgggaag agtgaacact tggctttcta cattccccat atctgttacg acgatagcaa
    54901 taatctgtcg attattcaag ggtacgatac gaaagccagt cagtcggcgg tcttttattt
    54961 caggtcccaa tgaaaaagtc gtatagcttg tcagctcaga taatatctca gcagattgct
    55021 taataatctc attgatttca tgaaattctc tgcctagaga ctgacgaatc agcagcaaat
    55081 catcttctgc tacgcgagca ggttttaaca aatgatccac gtaataacga tagcctaaag
    55141 cagatggaat ccgcccagaa gaagagtgag tcttcaacag caatccttca tcttctaatg
    55201 ctttcatatc attgcgaatc gttgctgagc tggccttgac tccctcagac attaatgttt
    55261 tggaaccaac gggcacgcca ctactcgtat agtgttggat gatcagacgt aaaatgtcgt
    55321 tttgtcgctt tgttaacatc ttcctcacct ctcattagca ctcatatatt ctaagtgcta
    55381 attcacttat aaatatagca agaagccctt ctcctgtcaa gagaaagact ccatttttta
    55441 gcactctaaa cacaagagtg ctaattctaa ataattgatg taaatccagt tatatcaata
    55501 cttcaatacc tcattgtcaa actttctaaa ctttttcttt tcaagtttcg ataaaaaaca
    55561 aatattcttc aaactatttt tcaaataaaa agcgctcaaa cacattgttc cccaaaaaag
    55621 tccctcttgg tgtcaaataa acacggtctt cttcattaac aagtaaccct tcttgctcta
    55681 attcccttag cacatcaccg tacaccgatt caattgtttt ttgatagcga tcatagaaat
    55741 gttttttagt aatccctgtt ttttttctta gtcctaagaa catttcttct tctatttgat
    55801 tttttcgtgt caatttttct tcttccaata ccgggcgttc atttgctcgt aatgggcgta
    55861 aatagtgttg gattggtcct ttgttgcggt aacgaatatc tccaagataa ccgctagcac
    55921 cagctccaaa cccaaaatag tggtcattgt tccagtacat gaggttatgt tgagattcat
    55981 gtccggagag cccaaagtta cttatttcat attggtgacg tccatgcttt tccattgctt
    56041 caatcgtctc ttcaaacatt ctggtttccg tttcctgatc aggcagttcg agtcttcctt
    56101 gtcgtaccca gttcatgaac attgttttgt tttcgaggat caatgagtac aaagaatagt
    56161 gaggcagatc caaagccaaa gctttttcca aagtatcccg atatccttct agtgtttgtc
    56221 ccggtaatgc ataaatcaaa tcgatgctta cattggtaaa attttctgct tctaaaaatt
    56281 gcattgtttg atacacatct tctgcagtat gtttacgtcc aatttttttc aataagcgat
    56341 tgtcaaatgt ctgcactccc atggataaac gattgacgcc atagtttttc atcacttgaa
    56401 gtttttcacg ggtcaaatca ccaggattcg cttctacagt aaactcatta ccattttgaa
    56461 aaggcaggat ctctctagca cctgataata gtcgatcaag ttgtttagca gataaagaag
    56521 ttggtgttcc gcctcctaca taaagtgttt ccaaattcgc tgtaggatga ctttccagca
    56581 ttaatctcat ttcattcagc agcatttcca catactcatc tactggctgc ccttctaaaa
    56641 aaactttatt aaagtcacag taatagcaga tatgctcgca aaatgggata tgaatgtacg
    56701 cagaaacctt gttttctgcg ggatttattg tttctatcat tgaaattctt tctccttacg
    56761 tcgaaaagtc gtgcctgttt tcgaccttct taaaatcgcg ttcaagcacc aactcattga
    56821 aataagccgc atatttagaa tcaaggaaac ctagctctaa aaatgcttgc ttattgtcat
    56881 cggagctaca cggtgctttt acagcctctc gataaacggt aatcgcaaag ctgaccttcg
    56941 gcattaagtc aaaatctgca aaaatatgaa aagctatttt cacagattcc tccttaagtg
    57001 ctcagaccac aacgctttgc ttacaacctc tcatatacgc atactaaacg tatttggcga
    57061 acaaatccac taaaaaatcc aagaatcgac ttcttggatg atttttttac actcatgatt
    57121 cttcctaaac aattttgtcc ttattttatt cataaaaaaa tctagtcaaa tctctcactt
    57181 caagagtttt aactagattc caattattat ttttttgtaa aaataaacag tctcttaatc
    57241 atagacattt cttcggtgcc ctacttgaat agcgataaca acgaattgat tatcttctat
    57301 ctctaccaat acacgtattt gccaatcaga gctgcttctg aatttcgggc gtgaatttta
    57361 aacggcaggt cattccagcc caccaaattc attaaggatt cttccatgaa tactgttgtt
    57421 ttcccacttc ttatccaatg tttatgggca atttcagccg tttgacgatt gtattcatct
    57481 tctagttgtt ccatcgaata gttttttaga agttcagata aagtaatccc ataaaattca
    57541 gccatataag aaagccattc ttttttggcg tcacttacac gaaccgtaat catagcccta
    57601 taattgcctc tcttaaagaa tctcatgcat ttcctgatgt gattttagaa aaatttactt
    57661 gctaaaaaac tgtgaaaatg taggagctag catccttgta gtaaatgatc gagctcttta
    57721 ttatacatta aaaaagttca gaaattttga tgaagtagcc aactactttt tcatgagtaa
    57781 agcaacacta gaaaaagaat cctccagtac ttagaattta ctggaggatg tacttttgaa
    57841 gaagcctata ggttatttaa aaaatattac tacaggaagg aacgagaatt acttcgttac
    57901 ttataataga acttagtctt atatagaagg catagctcac attagcttat ttgtcctata
    57961 aaaataaatt acggtatttt agaaaagcta ttttctcttt tttcaacgat cttcagcaat
    58021 ttttctttat cgtcaggtaa ctcaagttca tgaattctag catgacaatt gggacataac
    58081 gcgatacagt tttcaatcac atcttttcca ccttgcgaca aatattctat atgatgacaa
    58141 tgtaaataag gtacaccgtc ttttataaat ggcccttttt cttggcaaag ttgacaaata
    58201 ccttttgcaa gtcgtttaac atattcagat acgaagggat tacgtttata tatttttgtt
    58261 tttgagaagc aaaactcctc ttcgttaaca tattttgcag ccattttttt attataatta
    58321 ctaacttttc ttgcttcttc ctctaattta gcattagata aattacgaat atgtctagtt
    58381 tgattttctt cctggttata tatctttatc ttcaatcaat tgctcttgat ttattaaagc
    58441 taatggaaat ttatatactt ttctgctgtt gcccgaacta tctttttcat taacagtata
    58501 tatttgtcct gctaaatata cttctcccag atatgtgtgt tcttttggtt taaatgtttc
    58561 aaataaatga atctttacaa aattactttt tgaattggct aaaactttat tttgattctt
    58621 atccaatact tgatcccctt ttagccccat accggtatat tgtaaaatac catttaacca
    58681 cttatcttta taaacattac ttcctgtttg atcactaaac agtacaagag tattagtttg
    58741 atgagatctt ctcattccac cctgtttgga acatttaaac ttctctgaaa tttctttgtt
    58801 agtatatact ctaccaactt ctaactcacc taatatgttt ttagaccaat tttctcgcca
    58861 tgtaataatc ttatcaagat taaacatttc tccctctttt ggcatgccat ttctaattaa
    58921 attagctaat acaccagtag aaatatctaa tttttttagt aattctttct tagtaatcaa
    58981 tgttggattc ataacaatgc agctcctaat gatatcaata taattgcatt agataacttg
    59041 aaaaatcgaa aaaaactcat tttgtgtcaa gttatctgta atatataaaa atatcattat
    59101 tatacctcat ctatgtgaat ttttactatt cttaccttag catattcttt ataggtatat
    59161 cagagctaat caaactaaaa aatacccgaa gtcttcttat gtaaaaacac cggttattac
    59221 ttaaatatga cttaggtaaa acaatgagtg atccaattat aaaattttat ggcgaataat
    59281 ttagtaagcg ctacatattg aatgatttag aaattttgaa aaagtagcta gttcctttta
    59341 taccagaaaa gtaaacctaa taaaaaacac aactaatttt tagctgtgtt ttttaagtta
    59401 caataattat ctatttaata gtcttaattc attaaaataa aaaactcaac tttacaagga
    59461 tatggtttaa aaaatttatt tatccaaccc aactagtttt cgctgcttac gatttgcttc
    59521 atctgctacg taattcatta ctgttttaaa ttctttcgct gaggcgtttt ctgataaatc
    59581 agcgatactt ttattatact tcttcacgat ttgttgtact tgttctttct cagtcatgta
    59641 attcctcctc caactctttt gaagtatatt gtattggtag tactaattga atattatctg
    59701 ctgcataatg cacttcagaa atgtcgtttt gataatcctt aatcattatg tgaaggacta
    59761 cttcaggatg tctatttttg actttttcaa tattctctct ggacttaaaa aacgcattga
    59821 taaacatttc ttttggcaca tgacgtcctt cagctagttc acgttttcga gtgaaatccc
    59881 aggcaacttt gggatcttgg taaacataga aaatcgtaac gcgaaagtta tttttaaggg
    59941 ctcgaataat atttttctct gcactaagga tagcaaaagt atcctctagt atgaaagaat
    60001 agccatgttc cagtacatat tttaatgatt gttcaactaa ccaagaagaa gctttctgaa
    60061 aaacacttga gttctttcca ttgtattctg gaaattgtgc tctaaaatag tccgcatcaa
    60121 tcacaacatg gttgtcataa ttttaagcta aacctagtgc aacctctgtt ttacctgctc
    60181 ctgggcttcc agccatgaat accgcatctt tttcatcatc agggattttt cccttgatga
    60241 ttgtttcaat aaatttatct ttattgaccc tagcgtaatc taaatacttg tctttaggtt
    60301 ccaagattgc accttctttc cttcttctat attcatacta attatacgat atttatataa
    60361 aaaatataac ggtcatttta accatattaa catttagtca cagtaatagc agatatgctc
    60421 gcaaaatgag atatgaatgt acgcagaatc cttgttttct acggcattcg cttttttttc
    60481 attggactag ttatatattt ctagggttgt tttcatgtct gagtgcccga tatgatttat
    60541 ttttcgtttc tataattact ttttgccttc cagtttcttt taagattgta ctcaagcaag
    60601 tgggaattgg ttcttttgtc atccatgtat gcaaaatgtt ataatgctgg taaacaaaaa
    60661 tgattacctg tcatttttat ttagttcgta tttaattaag ttgactgtct gtatactctg
    60721 tctaaaactt aagaattgtt gttttcgctg tcgctatcca aatttaatga atgtctgtat
    60781 gttaaaactg ttgtccttgt catagatagt aaaggagagc ttgaaaatga aaactgcgat
    60841 tacgtttcta ttagaggaat atgccgattg ggaaggtgct tatattacta gcacactaaa
    60901 ccaaaaagag ggatggcaaa acaaaactgc ttctatccaa tcagaggtca cttccattgg
    60961 aggattaaaa acaaaaatcg attacttaat cgaacaaata cctgaaaaag ttgacctttt
    61021 tattatgatc ggtggcaatt catgggacat aaaaaatcat catttatatc aaataatcga
    61081 taaattgtta aaaacagacg taccagtagc tgctatttgt ggggcagttg attatttagc
    61141 tcagagcgga ttattgcaag gttacaaaca caccggaaat gcgcaatttc cttggttaga
    61201 atcggaaaat tatcagaata aagaagattt tatagaacaa caagctgtga aagatcgtaa
    61261 tttgattact gcgaatggaa cagctcctat agaattcaca gacttgattc ttcaagcagt
    61321 ccattttgaa acagaagaaa taagagaaaa aaagattcgt ctatacaaac ttggattcta
    61381 tgattactat aaaaagtacg gagatccttt ctcctaatca ttctcttcaa ttaaatactc
    61441 cttatcaata gtatgacaag taattttact gatggttatt tgtcacactt tttctgacat
    61501 ttatcaaaga agatacactc tatttatatt catagatgga atggcaacag tttttttgac
    61561 aaattttata aggtgcagaa cttctttccg tatgctattc cgattgtcct tgacaatgag
    61621 cctcctcgga tgtgacgatc ttcggcagga gctaacagtt aaagttagac tgcctcgcta
    61681 gcgttagcac atccgagctc attatcaagg attcgctgc
//
LOCUS       DAZUMM010000004        61714 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000004 DAZUMM010000000
VERSION     DAZUMM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.004"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..511)
                     /locus_tag="ACG8E6_000195"
     CDS             complement(134..511)
                     /locus_tag="ACG8E6_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060649.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            767..1495
                     /gene="purC"
                     /locus_tag="ACG8E6_000196"
     CDS             767..1495
                     /gene="purC"
                     /locus_tag="ACG8E6_000196"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFS5060650.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1495..1749
                     /gene="purS"
                     /locus_tag="ACG8E6_000197"
     CDS             1495..1749
                     /gene="purS"
                     /locus_tag="ACG8E6_000197"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFS5060651.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1751..2422
                     /gene="purQ"
                     /locus_tag="ACG8E6_000198"
     CDS             1751..2422
                     /gene="purQ"
                     /locus_tag="ACG8E6_000198"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFS5060652.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2423..4645
                     /gene="purL"
                     /locus_tag="ACG8E6_000199"
     CDS             2423..4645
                     /gene="purL"
                     /locus_tag="ACG8E6_000199"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFS5060653.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4630..6069
                     /gene="purF"
                     /locus_tag="ACG8E6_000200"
     CDS             4630..6069
                     /gene="purF"
                     /locus_tag="ACG8E6_000200"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFS5060654.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6092..7144
                     /gene="purM"
                     /locus_tag="ACG8E6_000201"
     CDS             6092..7144
                     /gene="purM"
                     /locus_tag="ACG8E6_000201"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFS5060655.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7141..7719
                     /gene="purN"
                     /locus_tag="ACG8E6_000202"
     CDS             7141..7719
                     /gene="purN"
                     /locus_tag="ACG8E6_000202"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFS5060656.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7716..9251
                     /gene="purH"
                     /locus_tag="ACG8E6_000203"
     CDS             7716..9251
                     /gene="purH"
                     /locus_tag="ACG8E6_000203"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFS5060657.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9267..10541
                     /gene="purD"
                     /locus_tag="ACG8E6_000204"
     CDS             9267..10541
                     /gene="purD"
                     /locus_tag="ACG8E6_000204"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFS5060658.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10663..11577
                     /locus_tag="ACG8E6_000205"
     CDS             10663..11577
                     /locus_tag="ACG8E6_000205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFS5060659.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11710..13275)
                     /locus_tag="ACG8E6_000206"
     CDS             complement(11710..13275)
                     /locus_tag="ACG8E6_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5060660.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13268..14719)
                     /locus_tag="ACG8E6_000207"
     CDS             complement(13268..14719)
                     /locus_tag="ACG8E6_000207"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5060661.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14733..15749)
                     /locus_tag="ACG8E6_000208"
     CDS             complement(14733..15749)
                     /locus_tag="ACG8E6_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5060662.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15969..17057
                     /gene="chvE"
                     /locus_tag="ACG8E6_000209"
     CDS             15969..17057
                     /gene="chvE"
                     /locus_tag="ACG8E6_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5060663.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17111..18637
                     /gene="mmsA"
                     /locus_tag="ACG8E6_000210"
     CDS             17111..18637
                     /gene="mmsA"
                     /locus_tag="ACG8E6_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5060664.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18639..19844
                     /gene="mmsB"
                     /locus_tag="ACG8E6_000211"
     CDS             18639..19844
                     /gene="mmsB"
                     /locus_tag="ACG8E6_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFS5060665.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20101..21078
                     /gene="guaC"
                     /locus_tag="ACG8E6_000212"
     CDS             20101..21078
                     /gene="guaC"
                     /locus_tag="ACG8E6_000212"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFS5060666.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21342..22766
                     /locus_tag="ACG8E6_000213"
     CDS             21342..22766
                     /locus_tag="ACG8E6_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS5060667.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22808..23065)
                     /locus_tag="ACG8E6_000214"
     CDS             complement(22808..23065)
                     /locus_tag="ACG8E6_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060668.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23227..24336
                     /gene="pdhA"
                     /locus_tag="ACG8E6_000215"
     CDS             23227..24336
                     /gene="pdhA"
                     /locus_tag="ACG8E6_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFS5060669.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24339..25316
                     /locus_tag="ACG8E6_000216"
     CDS             24339..25316
                     /locus_tag="ACG8E6_000216"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFS5060670.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25341..26984
                     /locus_tag="ACG8E6_000217"
     CDS             25341..26984
                     /locus_tag="ACG8E6_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFS5060671.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26992..28398
                     /gene="lpdA"
                     /locus_tag="ACG8E6_000218"
     CDS             26992..28398
                     /gene="lpdA"
                     /locus_tag="ACG8E6_000218"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFS5060672.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28560..28835
                     /locus_tag="ACG8E6_000219"
     CDS             28560..28835
                     /locus_tag="ACG8E6_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFS5060673.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28841..29608
                     /locus_tag="ACG8E6_000220"
     CDS             28841..29608
                     /locus_tag="ACG8E6_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFS5060674.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29776..31605
                     /gene="typA"
                     /locus_tag="ACG8E6_000221"
     CDS             29776..31605
                     /gene="typA"
                     /locus_tag="ACG8E6_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFS5060675.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31866..33638
                     /gene="recQ"
                     /locus_tag="ACG8E6_000222"
     CDS             31866..33638
                     /gene="recQ"
                     /locus_tag="ACG8E6_000222"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFS5060676.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33752..34054
                     /locus_tag="ACG8E6_000223"
     CDS             33752..34054
                     /locus_tag="ACG8E6_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFS5060677.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34057..35220
                     /locus_tag="ACG8E6_000224"
     CDS             34057..35220
                     /locus_tag="ACG8E6_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS5060678.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35246..38674
                     /locus_tag="ACG8E6_000225"
     CDS             35246..38674
                     /locus_tag="ACG8E6_000225"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFS5060679.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38695..39819
                     /locus_tag="ACG8E6_000226"
     CDS             38695..39819
                     /locus_tag="ACG8E6_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFS5060680.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39800..40246
                     /locus_tag="ACG8E6_000227"
     CDS             39800..40246
                     /locus_tag="ACG8E6_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS5060681.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40230..40568
                     /locus_tag="ACG8E6_000228"
     CDS             40230..40568
                     /locus_tag="ACG8E6_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFS5060682.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40687..41247
                     /gene="rsmD"
                     /locus_tag="ACG8E6_000229"
     CDS             40687..41247
                     /gene="rsmD"
                     /locus_tag="ACG8E6_000229"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFS5060683.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41244..41735
                     /gene="coaD"
                     /locus_tag="ACG8E6_000230"
     CDS             41244..41735
                     /gene="coaD"
                     /locus_tag="ACG8E6_000230"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFS5060684.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41725..42768
                     /locus_tag="ACG8E6_000231"
     CDS             41725..42768
                     /locus_tag="ACG8E6_000231"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFS5060685.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42927..43655
                     /locus_tag="ACG8E6_000232"
     CDS             42927..43655
                     /locus_tag="ACG8E6_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFS5060686.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43771..44016
                     /locus_tag="ACG8E6_000233"
     CDS             43771..44016
                     /locus_tag="ACG8E6_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS5060687.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44013..44231
                     /locus_tag="ACG8E6_000234"
     CDS             44013..44231
                     /locus_tag="ACG8E6_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS5060688.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44367..44906
                     /locus_tag="ACG8E6_000235"
     CDS             44367..44906
                     /locus_tag="ACG8E6_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFS5060689.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44899..47172
                     /locus_tag="ACG8E6_000236"
     CDS             44899..47172
                     /locus_tag="ACG8E6_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFS5060690.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47210..48256
                     /gene="holA"
                     /locus_tag="ACG8E6_000237"
     CDS             47210..48256
                     /gene="holA"
                     /locus_tag="ACG8E6_000237"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFS5060691.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48345..48599)
                     /gene="rpsT"
                     /locus_tag="ACG8E6_000238"
     CDS             complement(48345..48599)
                     /gene="rpsT"
                     /locus_tag="ACG8E6_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFS5060692.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48999..49268)
                     /locus_tag="ACG8E6_000239"
     CDS             complement(48999..49268)
                     /locus_tag="ACG8E6_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060693.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49530..49658)
                     /locus_tag="ACG8E6_000240"
     CDS             complement(49530..49658)
                     /locus_tag="ACG8E6_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060694.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(49738..50193)
                     /locus_tag="ACG8E6_000241"
     CDS             complement(49738..50193)
                     /locus_tag="ACG8E6_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFS5060695.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50331..52259)
                     /locus_tag="ACG8E6_000242"
     CDS             complement(50331..52259)
                     /locus_tag="ACG8E6_000242"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5060696.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52456..53289)
                     /locus_tag="ACG8E6_000243"
     CDS             complement(52456..53289)
                     /locus_tag="ACG8E6_000243"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFS5060697.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53481..54185)
                     /locus_tag="ACG8E6_000244"
     CDS             complement(53481..54185)
                     /locus_tag="ACG8E6_000244"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5060698.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54208..54834)
                     /locus_tag="ACG8E6_000245"
     CDS             complement(54208..54834)
                     /locus_tag="ACG8E6_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5060699.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55160..57535
                     /locus_tag="ACG8E6_000246"
     CDS             55160..57535
                     /locus_tag="ACG8E6_000246"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFS5060700.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57559..58050)
                     /locus_tag="ACG8E6_000247"
     CDS             complement(57559..58050)
                     /locus_tag="ACG8E6_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFS5060701.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58237..59157
                     /locus_tag="ACG8E6_000248"
     CDS             58237..59157
                     /locus_tag="ACG8E6_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5060702.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            59311..59655
                     /locus_tag="ACG8E6_000249"
     CDS             59311..59655
                     /locus_tag="ACG8E6_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFS5060703.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59845..60345)
                     /locus_tag="ACG8E6_000250"
     CDS             complement(59845..60345)
                     /locus_tag="ACG8E6_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5060704.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60873..60956
                     /locus_tag="ACG8E6_000251"
     tRNA            60873..60956
                     /locus_tag="ACG8E6_000251"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60907..60909,aa:Leu,seq:cag)
     gene            complement(61280..>61714)
                     /locus_tag="ACG8E6_000252"
     CDS             complement(61280..>61714)
                     /locus_tag="ACG8E6_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS5060705.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        20243 a        10663 c        13217 g        17591 t
ORIGIN      
        1 accaaaaaag aagcagatcc gttagcggtt ctgcttcttt tagtttatta tattttctta
       61 tcaataatag gaacctgatt ttaaaagcgg ctacctcttt accgtgcctc tttacataca
      121 aaaaatagag gaattaagcg taaatggctt ggctattata actaaaatgc tggttatgga
      181 gatgttgcag caagttttcg agaaaagcgt attccatctc ttgtccattg aataagatct
      241 ggatacgata atgatttttg aaacgcttct gttcatgaat ctgactgatt gaaagttcgc
      301 ctttgtgttc tgtctgataa agaaaagtcc ttttacggca ttcagcacag cgcttggttg
      361 ctcggcggat ttttcggaat gtataaaaac tagtatcttt attgcataaa taaacgtctt
      421 tgatcgacag caaatcatta tatagacagg aacgttcttc agctgttaat tctgataaaa
      481 aaagctgacc ttgttttatt gcatcttcca tcggcaagtg cctcctcgaa cccattatcc
      541 aaatttttag ttaattctaa tataccatca ttaagtataa atgtaaacgc ttgttgatgg
      601 tataagattt tcgtttctga taaaacacga acattaaaag aatctaatgc cttaaatgta
      661 catatttttg ttgatttata aaaagatgat tgctaaaatt taaagtgaaa aaacgaacaa
      721 aaaacgatgt atttgctttt aatgtaaata aaaaggagag acgtacatgg agaagaaaga
      781 acttttgtat gaaggaaaag ccaaaaaaat atatcttact tctgatgaga acttgatttg
      841 gatagaatat ttagatcaag caactgcgtt gaatggcgcg aggaaagacc agataagtgg
      901 aaaaggagaa ttgaataatc aaatcacaag tgcaattttc cgttatctga ctcaaagagg
      961 aataaaaaat cattttattg aacaattgtc taaaaccgaa caattagcaa aaaaagtaca
     1021 aattatccct ctggaagtgg ttatccgaaa tattgcagca ggaagtttct caaaacgtct
     1081 aggtgttccc gaaggtaaag aattcatcaa accaatcatt gaattctact ataaggatga
     1141 ccagttggat gatccattta tcaatgaaga gcatatctat ttcttacaac ttgcaaagcc
     1201 ggaagaaatc agtgagctga aagcccaagc gttggctatt aatgaaattt tgaccgaatt
     1261 atttgctaag gtcggtttgg ttttagtaga tttcaagcta gaatttggcc ggtcgaatgg
     1321 acaaattttg cttgcggatg aagtaacacc ggatacttgc cgattatggg atgaacgtac
     1381 gaatgaacat atggacaaag acgtttaccg cagagatcta ggtgatttga tcccagtcta
     1441 tcaagaagta tataaccgtc tatcggaact aactgaagaa aaggagcgag tatgatgtat
     1501 ttcgtaaaag tatatgtaac ctataaagat tcagtgttag atcctcaagg ggaggcagta
     1561 aaaaaagcag tccatcgtat gggatatgaa acaatcgaag atatccgaat aggaaaatac
     1621 tttgagatac aacttgctga tgcagaagaa ccaatcgata cagtgattga aacgatttgt
     1681 gacaagctgc ttgcaaatgt aaatatggaa acataccgat atgaaatcaa aaaaatggag
     1741 gaagtataac atgaaatttg cggtcatcgt ttttccaggt tcaaattgtg acgcagatat
     1801 gttatgggca gttcgcgaga tcatgggcgc tgatgccgaa tttgtacggc atgatgagtc
     1861 ctctttagaa ggatttgatg gggttttact gccgggagga ttctcctatg gtgactattt
     1921 gagatgcggt gcgattgctc ggttttctac aatcatggaa gaagtgattc gttttgctga
     1981 agaagggaaa ccagtattcg gtacatgcaa tggattccag atattgacag aagcaggact
     2041 tttgccaggt gtactgagac gaaatgagtc tttgcatttt atttgcaaaa ctgtaccatt
     2101 gaaagtcaac cccaagacgg cctttacttc agagtatgaa gccgacgaaa taatctatct
     2161 tccagttgct catggggaag gaaattattt ctgtgacgaa gaaacacttc aagaactaaa
     2221 aaaaaataat cagattgcct ttacttataa tgacaacatc aacggcagcg tggaaaacat
     2281 tgcagggatt acgaacaaag cagggaatgt gttaggaatg atgcctcatc cagaacgtgc
     2341 agtagaagac ttgctaggat cagaagatgg gaaacggttc tttgcttcca ttttaaaaaa
     2401 cttcggaaag gtgaccgtat agatgaaaac attgaaagaa ccgacagctg aagagatcaa
     2461 gaaaaaaaga atctatgccg aatggggatt gactgatgaa gaatatcgaa tgatcgaaga
     2521 agatatctta ggaagaatgc ctaattatac ggaaactgga ctgttttctg ttatgtggag
     2581 cgaacattgt tcctataaaa attctaaacc ggttctacgc aaatttccta ctagtggacc
     2641 tcatgtgttg caaggccctg gagaaggcgc aggcatcgtc gacatcggtg atggactagc
     2701 agttgtcttc aaagcagaaa gtcacaatca tccttcagcc gtggagcctt atgaaggagc
     2761 tgcgacagga gttggcggaa tcatcagaga tattttcagc atgggggctc gtccaattgc
     2821 gttgctagat tcattgaggt tcggcgaatt agataatcca agaacgaaat atttactaga
     2881 agaagtagtt gcaggtatcg gcggatacgg caattgtatc ggcataccga ctgtaggcgg
     2941 cgaaattgca tttgatcctt gttatgaagg aaacccatta gtcaatgcaa tgtgcgtagg
     3001 tttgattgaa cacaaacata tccagaaagg gcaagccgct ggcgtcggta attcgatcat
     3061 gtatgtaggt gcaaaaacag gtcgcgacgg tatccacgga gcaacttttg cctcagaaga
     3121 atttaatcaa gaagaagaac agcaacgttc tgctgtacaa gtaggcgatc cttttatgga
     3181 aaagctgctg cttgaagcgt gtttggaatt aatcttgcat cattcggatt ctctaatagg
     3241 tatacaggat atgggagcag caggacttgt ttcatctagt gcggaaatgg cttcaaaagc
     3301 tggctcagga cttatattga cattagatga agtcccacaa agagaaactg ggatgacacc
     3361 ttatgaaatg atgttatcgg aatcacaaga acggatgttg atttgtgtta aatcaggaga
     3421 ggaagaaaaa atccaagaac tatttcagaa gtatgatcta gatgcagtca ctatcggtaa
     3481 agtaacagac gacgggcaat atcggctata tcatcaagga gaagaagtag caaatctgcc
     3541 agtcgatgca ttagctgaag atgcaccagt ttatcataaa gaaatgaaag aaccagctcg
     3601 aatcgtagaa tttcagcagt tagctcctta tcagccagtt atcgaagaac ctggacaagt
     3661 attgcttgat ctgctgcagc aaccgacgat tgcttcaaaa cgttcaatct acgaaactta
     3721 tgattcacaa gtacaaacca atacagttgt ccgtccagga agcgatgctg ctgtgatgag
     3781 ggttagaggg acgaataaag cgttagctat gaccacagac tgcaacgcgc gctatctgta
     3841 tttggatcca aaaatcggag gacaaatagc agtggcagaa gctgctagaa atattattgc
     3901 tagcggtggt aagcccttgg cgatcaccga ctgcctgaac tatggttctc ctgataaacc
     3961 agaagttttt tgggaattat cgacttcagc tgacggaatt gctgctgcgt gtgaaacgtt
     4021 aggaactcct gttatttcag gaaatgtctc tttatataat gaaacggatg gtcaagcaat
     4081 ctatccaaca ccaatgattg gtatggtcgg attgattgag gatcataaac atatcactac
     4141 tcaggaattc aaaaaatcag gggatctaat ctatatatta ggtaagacgt ttgcagattt
     4201 tgatggcagt gaattacaaa agatgcagct tggaagaatc gaaggtgtga tccgaaactt
     4261 tgatctatcc attgaaaaga gaaaccaaga gttagtattg acagccatcc aaaacggatt
     4321 gattgaaagt gctcatgact gttcagaagg tggcttggca attgcactgg cagaatcagc
     4381 tttcaaacat cagctaggga tatctgtaca gtttgaattg tcctctgctc aattatttgc
     4441 agaaactcaa tcaagatttg tgttgacagt cgcaccggaa aataagacac gattcgaaga
     4501 aatgatggga gatgctgctg ttttagcagg taaggtgacg gatgaagcga taatcgaaat
     4561 ttcagcaact gacggtcaga tcaaaataga aactgcagta gcaagaaagt gctgggagga
     4621 tgcaatcgta tgtcttatga agtgaagagt ttaaatgaag aatgcgggat cttcggtgtt
     4681 tggggtcatc cagacgccgc tagtgtggca tacttcgggc tccatagtct tcagcatcga
     4741 ggacaagaag gtgcaggtat cgtgtccaat gatcgaggaa agcttaaggg gcatcgagat
     4801 ttggggctgc tttctgaggt attcaaagat caaagaaaat tgcagcagtt aacaggagaa
     4861 gctgcaatcg gacatgtacg ttatgcaaca gcaggcaatg gaagtgtgga caatattcag
     4921 ccttttctat tcaaatttca tgatcagtcg atcggcttag cgcataatgg taacttgatc
     4981 aatgctcgtt cccttagaaa agagttagag gaaaatgggg cgatcttcca ttctaactcc
     5041 gatacggaga tattgatgca tttgatccga cgaagtgaag aaacagattt tttaaataaa
     5101 ttaaaaagtg ctttgaatga agtcaaaggt ggctttgcct atttattaat gacagaaacg
     5161 gcaatgattg ccgctttgga cccaaatggt tttcgtccat tagcaatcgg acaaatgaaa
     5221 aacggagcat acgtgattgc ctcggaaact tgtgcattag aagttattgg ggcacgtttt
     5281 ataagagatg tccagcctgg tgagatagtg atcatcaatg acgaaggaat ccagattgat
     5341 tgctttacga aggacacgca gctttctatt tgttcgatgg aatatatcta ttttgccaga
     5401 ccagattcaa acatcgcagg gatcaatgtg catactgcac ggaaaaatat gggacggaat
     5461 ttagcgaaag aatcgccagt aaaagcagat atggtcgttg gcgtacctaa ttcttctttg
     5521 tcagccgcaa gcggttatgc ggaagctagt ggaatccctt atgaaatcgg attagtaaaa
     5581 aatcaataca ttgcccgaac atttatccag ccaacgcctg agctaagaga gcaaggtgtt
     5641 cgaatgaaat tatctgctgt acgaggagta gtggaaggta aaaaggtgat tatggtcgat
     5701 gattcaattg ttcggggtac gacaagcaga cggatcgtcc atcttttgaa agaagcaggg
     5761 gcaaaagaag tccatgtgag gattgcttct cctcctttga aatatccatg tttttacggg
     5821 attgatattc aaacgcgtaa agaactcatt gcggcaaatc acacgattga tgaaatcaaa
     5881 gaatgtatcg gtgcagattc tttaggtttt ttaagtgaag agggtctgat cgaatcgatc
     5941 ggtctggaaa aagatgctcc gtattcagga ttgtgtatgg cttatttcaa cggagattat
     6001 ccgacgccgt tatatgacta tgaagaaaaa tatcaggaat cactaaaaga aaaagtatcg
     6061 ttcttttaaa agcagaccaa gaagggagta tatgaattca atggcaaatg catacgcaaa
     6121 agctggcgta gatgtcgaag ctggatatga agtagtagaa cggatcaaga agcatgtcaa
     6181 aaaaacggaa cgtttaggta tgatgggcgc tataggagga ttcggcggtt gttttgattt
     6241 atcggtcttt caattaaaag agccagtcct tgtctcgggg accgatggcg tggggacgaa
     6301 attagcagtg gcaatcgaag cagacaaaca cgatacaatc gggatcgact gcgtagcgat
     6361 gtgtgtcaat gatatcttag cacaaggcgc agagccgtta tatttcttag actacttagc
     6421 aacggggaaa aatgatccgt tacgtttgga aaatgtagta aaaggtgtag cagatggatg
     6481 tgttcaagga aatatggcgc tgattggcgg agaaacagct gaaatgcctg gtatgtactc
     6541 aggtaatgat tatgatctag ccggatttgc tgtagggata gctgaaaaat ctcaattatt
     6601 gacaggagaa acgatcaacc caggagatgt gttgcttgga atagcatcta gcgggattca
     6661 ttcaaatggg tactctcttg ttcgcaaaat tttctttgag cagcagcagt tttctttatc
     6721 atcaaaactg ccagaattga aaaaaacact tggcgaagaa ttgcttgagc caacgaaaat
     6781 ctatgtttct tccttgcttc cgctgatcaa agaaaagaaa ataaaaggag ctgcacatat
     6841 cactggtgga gggtttgtag aaaatatccc gcgaatgtta ccagaatcta tggctgcaca
     6901 aatcaaactt ggctcatggc cgattcttcc aatattcggc tgtttacaaa aatatggtga
     6961 aattcctgag atggagatgt atgaaatctt caatatgggc atcggtatgg tcctggcagt
     7021 cgaagaatcg aaagtaaaag aaatagtaca cgaaattgag caagctggcg aaaaagttta
     7081 tgaaatcggg aaagtgatcg ccaaagaaaa acatcgtgtg gtgtttatag gagaagaacg
     7141 atgagaatcg ctgtttttgc ttctggaaac ggaagcaact ttcaagcgct agccgattat
     7201 ctatcgaaaa aaggattgga atcttcgatt gactggttgt tttgtgatca gcctgaagca
     7261 tatgttttga aacgtgcaac tgcgttgagt gtaccggcag attgtttttc acctaaagaa
     7321 tttgattcaa aaaaagagta tgaagaagca attttgcata aattgaaaga aaaaaagatc
     7381 gatttgatcg tattagctgg atatatgcga atcattggac cggtattgct gaaaaattat
     7441 gataaaagaa tcatcaatat ccatccgtct cttttacctg ccttccctgg tttgcatggc
     7501 atacgtgatg cctttgaagc tggagtaaaa gaaacgggtg tcacaattca ttatatcgac
     7561 caaggtgtag atactggacc aatcatccgg caggaaaaag tgagaattga acaggaggac
     7621 acgttcgatt ctttagaaga aaaaatccat agagtagagc atcgaatcta tccagaagtg
     7681 attagtgaaa ttattgaaaa tggaggctat ctgaaatgac aagagcattg atcagtgtat
     7741 cagataaaac tgggattgta gcattcgccc aaggtttgat tgaacaagga gtagaaatca
     7801 tttcaacagg aggaacaaag catgcgttgg ataaagctgg gatcaaaacg ctttcgatcg
     7861 aagaagtgac agggtttcct gaaatgatgg atggccgtgt caaaacactt catcctaaaa
     7921 tccatggtgg tcttttagga agacgcgatc tccctagcca tatggaggca atggaaacgg
     7981 aaaatatcca accaatcgac tttgtttgtg tcaatttata tcctttcaaa gaaacgatta
     8041 tgaaaccaga tgtcgaaata gcagaagcta ttgaaaacat tgatatcggt ggtcctagta
     8101 tgctccgtag tgcagcaaaa aaccatgaat ttgtcacctc tgttgttgat ccatctgact
     8161 atgaagaagt actttcagaa ttacgtcagt atgggcatac ttcactttct ttcagacaaa
     8221 aactagcggc aaaagtcttt cgtcatactg cggcatatga tgcactgatc gctcaatact
     8281 taacggattg ggtggaagaa aaagaaccgg aaaaattgac acttacgtat gaacgaaaac
     8341 aaacgcttcg ttacggtgaa aatagccatc aagccgctgc cttttatcag tccgctcttc
     8401 tggaaaaata ttcgatcgca gcagcaaaac aactccatgg gaaagaactt tcttataata
     8461 atatccgaga tgcagatgct gcgctacgga tcatgaagga atatgacgag ccaacggtag
     8521 tcgccttgaa acacatgaat ccttgtggta ttgggacagc atctacgatt ttagaagcgt
     8581 gggaagcagc gtatgaagca gatcctgttt caatctttgg tgggatcatc gttttgaatc
     8641 gagaagtaga tctgccaaca gcagaacaga tgcataagct gtttctagaa atcatcattg
     8701 ccccttctta tacacaagaa gctttggaat tactgcaaaa aaagaaaaac atccgattac
     8761 tgacagtaga ctttgcgcag gaagaaaatc aaaatcgaga agaaacggtc tcagtcttag
     8821 gtggacttct tgtacaagat caagatctgg caatagaaaa tgaagaagag tggaaagtcg
     8881 tgacaaaacg taagccgact ccagcagaag caaaagcatt agcgtttgct tggagagctg
     8941 tcaaacatgt gaaatctaat gcgatcgtac tggcaaacga acgtcagaca gtaggaatcg
     9001 gagccgggca gatgaatcga gtcggttcag taaaaattgc gattgaacaa gcgcaagaga
     9061 aaatggaagg tgcagtttta gctagcgatg cttattttcc aatggatgat tcggttgaat
     9121 atgcagccaa acatggtata aaagcaatca ttcagccagg aggaagcatc aaagaccaag
     9181 attctattga tatggcgaat aaatacgatc tagcaatggt ctttactgat gttcggcatt
     9241 ttagacatta gagaggagtc ggataaatga acattttggt gatcggtgca ggtggtcgtg
     9301 aacatgcaat aagtaaaaaa cttttagcca gtccgaaggt ggatacggtg tattgtgcgc
     9361 caggaaatcc ggggatgaca aaagatggta tcaattgtgt cgctatcagt gagcgagatc
     9421 attcagcgtt aatccatttt gttaagaaag aggctattga ttggagtatc gttggtcctg
     9481 aagttccttt gttaaatggg atcgtcgatg attttatggc ggaaggattg aaaatcttcg
     9541 gtccgacaaa agcagcagct ctgatcgaag gttcgaaaag ttttgctaaa caattaatgg
     9601 atacttatca aatcccgaca gctaaatcca gaagttttga atgttacgaa aaagcagcag
     9661 cgtatatcaa agaaaatggt gcgccaatcg tagtgaaagc ggacggctta gcagctggga
     9721 aaggtgtagt cgtggctacc acagtgaagg aagcgctgga tgcggcagaa caaatgttga
     9781 accagcatcg atttggggat agtagcaaaa aaatcgtcgt agaagagttt ttggcaggag
     9841 aagaattctc ccttttggcg tttgtccgta ataccaaggt gtatccgatg gtcatttcgc
     9901 aagatcataa gcgggcattt gataacgata aagggccaaa tacaggcgga atgggtgctt
     9961 attcaccagt accgcaaatt tcaaaaaaga tgattgaaaa agctgtggaa gaagttttaa
    10021 aaccagcggc tttgggaatg aaagaactag gacgctcttt cacgggtgtt ttatatgcgg
    10081 gtttgattgc tacgaaggaa ggaccaaaag tgatcgaatt caatgcgcgt ttcggtgatc
    10141 ctgaaacgca agtcgttctt tctcggttga agagtgactt tgcgcaagta atcgatgatt
    10201 tattagagaa tcgtacagta gagttaaagt ggcagcaaga aggatacaat gtaggcgttg
    10261 tggttgcagc tgccggttac cccgaagaat atgagacagg aatggttcta ccagattttc
    10321 tggaagaaga gctttctgtt tactatgctg gagtttcctc aaataacggc aaactagttg
    10381 gagcaggagg acgtctttat ttagtagaag catacggtga gacgatcaaa gaagcacaga
    10441 aaaaaattta caccgcgtta atggataaaa cgacagatgg aacattttat cgtacagata
    10501 ttggagaaaa agctgccaat agcgaactga tcgtgaagta aatcagctgc ttccatttat
    10561 aaaaaataca acaaacataa tcggaagcgc cgattttagt gaagcctttt cataaagagt
    10621 gtggtacact tttgttgtgt atataaataa ggaggagttc agatgaaaaa aattcaatta
    10681 ggtacttctg atcagaaagc agctgcagtg atcttaggct gtatgcgtat caacaatgcg
    10741 gaagatccag taaaagtgat tgagacagct tatgaaaacg gcattgattt ttttgaccat
    10801 gcagatattt atggaaacgg tgaatgtgaa gaaatctttg cagatgctct agcaaaaaca
    10861 tcaatcaaac gagaagatct ttttattcaa acaaaatgtg gaatcgttcc aggtgtgatg
    10921 tttgattttt ctaaaaaaca tatcattgaa tctgtagaaa aaagtttgac ccgtctgaaa
    10981 atggattatg tggatgcttt acttcttcat cgtccagata cattggtaga accagaggaa
    11041 gtagctgaag cgtttgatga attggaacgg tcaggaaaag ttcgttattt tggcgtcagc
    11101 aatcaaaaac caatgcaaat cgaattgctg aaaaaatatg ttcgtcaacc attagtagct
    11161 aatcagctgc aatttggctt gaaacatact ggaatgatcg atcaagggat tcatgtcaat
    11221 atgacagatg aagcaagtat cgatcatgac ggtagtgtac ttgattattc ccgattgcat
    11281 gatatgacga tccaagcatg gtcaccatac cagtatggat tttttgaagg tgtatttatc
    11341 gggaacgaaa aattccctga attaaaccaa acattagaag aactagcagc taaatatcaa
    11401 acgacaccaa ctggcttggc ttctgcttgg attttacgcc atccagccaa tatgcaagtg
    11461 atcgccggta cgatgaacgt agggcgaatc aaggaaatcg ctcaagcttc agaaattaat
    11521 atgtctcggg aagattggta cgaattgtac cgtgctgctg gaaacatttt accttaaaga
    11581 ttgtcataag caggtcaaaa gggctataaa ttaaaaagac agaataactt gaaagctgtt
    11641 ttcagcttcg agatattctg tcttttttta ttgcaaatgg aaagagtatc attgtacgga
    11701 tttcgtttat tagttatttt tgtttttaag gaaaagctgt gtccgatatt cttttggaga
    11761 aatcccttgt tttttcttaa ataaataact gaaatagtta gggtcattat aaccaatttc
    11821 aaaagcaata tctgaaatac gatagtctga ttgctgcaat aatttttttg ctaattcgat
    11881 tctttgttct gtcagataat cgatgaatgt ttggttaaac gattgagaaa aaatcgtact
    11941 gaaatgagag ggacttaatg caacttgttg agcgacccaa gttaaggaca tatctggatc
    12001 ggtaaaattt tctttgataa aatccaatgc atggttcaaa attggctgat atttgttcct
    12061 tgaaggctgt gagggcagag cagttaagaa agaaatgaac atataaactg tcgaacggta
    12121 aagcgagtcg tcggcgctga ttgctaagcg ttgactggtg gatgtgcaat gaaccatttc
    12181 ttgcgggatt ttcttttctt tcaggcagac atgctggagt agcttagtca agacaaataa
    12241 acgatataat cgtgttcgag ttggtgtgcc ttgtggtttc gtcaagtgtt cgatgtactt
    12301 agatacttcg tttttcgata atgaagacaa atcaagtgag aggtcaaagt aaaagacaga
    12361 atcaactgga agtttcgctt cttgaatcaa atcctcatag ctgataattt gttctttaag
    12421 ggaactggcg gtttcaagta tcatctgcgt gatctcaaaa gatgcaggta attcgctgat
    12481 tctttctgcg acaggaccaa tggcgacaga aaaattattt ttttgtccct tttctaattc
    12541 atggaccaac gtttgggcta cctgataact ggtctcgatc acattttctt caagagaatc
    12601 aactattaaa aatttgatga atcgagagga aacagcagag aagatcactt gttgattctc
    12661 cctaaataaa aacttcaaat aatcactgag atgagcgtaa tcttcaaagt ctgtactgac
    12721 ttggtttgct gctaataata cggtcatttt tttccctgct aaagagcgat tgaaagattc
    12781 agcaagtttc aatgtttcac tgatcgtaag tgagcctttg aacaaaccgt tcaagaaatg
    12841 attttttttc aattcgaaaa caagatcaga agaagtgtgc gtttcttttt gctgcaaatc
    12901 taaagcagtt actgcttttt ctattgcttg tttcaattcg ctacttttaa tcggtttaag
    12961 cagataatca tctgcctgta cctggattgc tcctttcata taatcgaaat cgtcgaatcc
    13021 actaataaat atgagacgag tccaaggaag tagtttcctt gcttcttttg aaaaagtcaa
    13081 cccgtccatg aaaggcattt ggatatcagt cagaatgatg tcgggctgta agtcgataat
    13141 tgaagacagt gccatctcac catctccagc ttcgccgaca taagtgattg gataataaga
    13201 accaaggaca gttatttgtt cccgcaagtt attgcgaatg aggtattcgt cttcgatgat
    13261 aaaaacttta tacatgtttg tttcctccaa ttgttttggg agatagaggg atcatcagtg
    13321 tgacagttgt tccttctcgg tatttgctgt caatcgtgat aggtttttga tcatttccgt
    13381 aataaagaag gagacgcttg tttacgttgt aaagtccgta gccgatactg aaatctgaat
    13441 cgatcccttt ggctaactct ttacgtattt ctgccagttt ttctgcaggg atgccaatcc
    13501 cgttgtctat aactttgaat tggatcatct catcaatttt gcatgtggta atacgaataa
    13561 atcctttgtg acggacgtac ttcgttccat ggtatacagc attttcaatc aatggctgca
    13621 agatcatttt taaaatagaa acaggaaaaa gtgtttctgg tatatcgatc ttatagtcaa
    13681 gcatatggcc gtatcttatt tttaagatga ctagataatc attgatatgg ctaatttctt
    13741 tttcaagagg aatccagtct tgtcctttgc ttaaagacgt acgaaaaaag ccggaaagtg
    13801 catgggtcat agtcagagct tcttcaaagc agtcttgttc gatcaaagaa ataatggcat
    13861 ccaaactgtt ataaataaaa tggggagtaa tctgagcttg caatacacga acttcacttt
    13921 gtgctaaatg gtattgtttc aaagcgttct cccgtaaaga ctggtttaga tcttccgcca
    13981 ttttattcat gctagcggta agtgtttgta attccagtgt ttccggcagg gttaaacgaa
    14041 agccaaggtt ccctgaagaa agttgttctg acatctcgat cagctgattg atcggttctt
    14101 ggacctttct ctctaaaaag cgacgattct ttttcgcata gaaaaaaatg gtactaagaa
    14161 ctccaagttc cactattgtt aatagaatga gcgagcggac aagtgtcgta ttttttttgc
    14221 ttgctaagtc gatctccaca cgaacaaaat cttgtaagat atctgacagc agttgagtga
    14281 cagaatctac ctgattgatg atctcttcat tggtatccat aaaatctttt gccaagatat
    14341 tattgatcag ctcgttttga taatcttcca gtgtatcgat cgtacgtagc gatacatcta
    14401 acgtactaat ttctttaagt gtatttgtat tatttttgat ctctcgaata tcattcctaa
    14461 gttcttctag gatattttcc tcattgaact catctacggt tacttggcca gaaatcaagt
    14521 cccacatact ttccaaaact ttttgatcaa ccttcaaagc aattgcatct gctttttgga
    14581 tatttgcgat cgtctgttga taaatcaaaa gctgttgcga atataacagt atgctcagta
    14641 gcaaagggat aacagcaaat agaagcatgg ctttgtttgc ttgggcaaaa atctgtttta
    14701 tgcttaaatt ttttgtcatg atacttctcc agtcaataat ttctggttgg gatgttagct
    14761 atattctgct gggaaaagac agtctcggaa ataaaaatat ctttaggtaa tgtttttcct
    14821 gtttgaatat ttccaaaata acgagtaacg gtattggcta cataccagcc gacatcaggt
    14881 ttacactcga ctacacaatt gacgattcct gagcgtagct tctcgatcat atcttcttgt
    14941 ccatcaatag ttacaatgat caaatcttct ccaggtttga ttgatgtttc ttttatcgca
    15001 gctaaagcac cgtgagtcat ttcatcacta tggctgtata atacatcgat ttcggataaa
    15061 tcatttgttt ctaaatatcg tttcatctgg tcttttcctt tcatccgaat aaaatcaccg
    15121 acaattcggt gaacaatagt catcggaggt ttatggtcga tggttccatc tttttttagg
    15181 gttggtttac gattgattga ttccaaaaat ccttcggagc gtagcttggt cggtgtggag
    15241 ttctctgaac cagcaagttc taggattctg atttcctgtt tctttgaatt tgtgaagtga
    15301 ttgctgacaa ataaacccgc ccgatttcct tctgctttga aactggggcc aatgtgggtg
    15361 acgaaaagct cgggatcatt cgtttgaata tgtctgtcga caagtatgac agggatccct
    15421 gctcgctttg cttcttttaa tacagagtcc cacccggatt cttgcaaagg agtaaaaatg
    15481 atcatatcca ctttataggc aatgaacgtc cgtatatctt ggatttgccg ttcctgattc
    15541 atgtagccat ttcggtagag aacttggtaa tcttcttttt caagcgcagt gataattgat
    15601 tcagtatgct ttttccgcca gctactctcc gttcctgatt gcgaaaaacc aacgactagt
    15661 gggtcaggtg cttcctttgt attaggtgta caccccatca tttggtaagc gattacaaca
    15721 agggctaaaa aaaataaaac acgtttcact ttaaatcact ccatctggat ttgttagtac
    15781 tatcttatca gataaaaggg aaaatcaaag tcaaaaggaa tcaatcgaaa aaaatccaag
    15841 aaacttaaaa aaatccaaga caaaaaaagt acacctaaaa aatttaaagt aaatcccata
    15901 aaaaaatcaa tgttaacgct ttctttatta gctatactcg gtgtatcaat tgaaatgggg
    15961 gataaaaaat gaaaaacaaa tttagaaaga ttgttgggac tatcgctatt ttgaccgcaa
    16021 gcattctatt aagtgcgtgt ggcaattcag gatctgcaga tgatagtacg gggtatgtag
    16081 gaatcgctat gccgaccaaa tcagcagaaa gatggatcgc tgatgggaac aacatggtct
    16141 cggaattaga aaaactgggt tataaaacag atcttcaata tggagaagat aaagtagaaa
    16201 atcaagtagc acaaatagaa aatatgatta caaaaggggt agatacactt gtgattgctt
    16261 ctatcgatgg ttcggcttta actgatgtgt tagctaaagc aaaagaagca gatatcaagg
    16321 tgatcgccta tgatcgttta ctgatgaatt cagaaaatgt cgattattac gcgacttttg
    16381 ataattttgg agtaggtgta tcgcaagcag cttatattga agagcactta ggattgaaag
    16441 aaggaaaagg accattcacg attgaattat ttggtggctc gcctgacgac aacaacgcac
    16501 taatcaatta caacggagtg atgtctgttt tacaaccata tatggacaac ggtcagttgg
    16561 tcgtgccatc tggacagacg agtttcagtc aaatcgctac gcttcgctgg gatggttcta
    16621 cggcgcaagc aaggatggat aatctgttaa gcgcaaatta tactgaccaa acattggatg
    16681 ctgttttgtc gccatatgat ccaataagtt taggaatcat ttcgtcatta aaaggcgtgg
    16741 ggtatgggag tgaaagtaaa cctttacctg tgattactgg acaagatgcg acagttgctg
    16801 gcgttaaatc gatcattgcc ggggaacaaa cacaaacgat ctttaaagac acacgtatct
    16861 tagcaaaaaa tacgattgaa atgatcaaag caatcagtga tggagaagag gttccggtca
    16921 atgatactga aacttatgat aatggggtga aaactgtccc tacgtattta gcaaatacag
    16981 tatctgtcga taaagataat tatcaagcag agctgatcga caccgattat tataaagaat
    17041 ccgatctgaa aaattaagag gaaccaaaca aggaactaaa gagatctaag tcagagagag
    17101 gataagaatt atggcggatt gtattttaaa aatggcagat atcaccaaaa aattttctgg
    17161 aatccgagcg ttagattgcg tgaatctgat gattgaacgt ggggaaatcc atgcattgtg
    17221 cggagaaaac ggagcgggaa aatcaacatt gatgaatgtc ttgagcggct tgtatccata
    17281 tggctcctat gacggggaaa tcttttatga tggaaagctg tgtcaattta aagatttgaa
    17341 agatagtgaa gaaaaaggca tcgtcatcat tcatcaagaa ttagcactaa gtccttatct
    17401 ttcaataaaa gagaatattt ttctcggaaa tgaacaagca gaaagaggcg tgattgattg
    17461 ggaactgaca gaaaagaaaa catatgaact gctgaataaa gtcggtttgt ctatcgaccc
    17521 gaatcttcta gtatcccaga ttggcgtagg gcaacagcaa ctagttgaaa tagctaaagc
    17581 attatcaaaa tccgtccgtc tgttgatttt ggatgagcct accgctgcat tgaatgaaga
    17641 agaaagcgcg aatttgctga gattgatccg agagttgaaa gagcaaggtg taacctccat
    17701 cattatttca cataagctga atgaaatcgt ggatatcgca gatcgaatca ccattttacg
    17761 ggatggacag acgattgaga cactagaaaa tgaaatgatc agcgaagagc atattattcg
    17821 cggaatggtg ggcagagatt tagttgatcg ttatcctgaa agaaaaccgg atattggaga
    17881 agtttatttt aaagtagaag actggacggt ctatcaccct cttgatcacg agcgaatcat
    17941 aaacgaccac atcaacttgc atttgaaaaa aggagagatt gtaggaatag ctggactgat
    18001 gggagcgggc agaactgaat tcgctatgag tctatttgga cattcttatg gaagcaagat
    18061 atccggacgt atattaaagg aaggggcaga gatatcagtt aagaccgttc cttcagccat
    18121 tgcaaacgga ctcgcttatg tctcggagga ccgaaaatct ttaggactga atctgctgat
    18181 ggatattcgg gaaaacgtga cgctgagcag tttggataaa ataagtcaaa aaggagtatt
    18241 agataaagaa aaagaagtaa ctgaggcaga aaatttccgg aaaaagatgc gtataaaaac
    18301 aaactccatc catcaaattg tcagtagtct tagtggtggg aatcaacaaa aagtcgtttt
    18361 atccaagtgg ctgatgacac aacccgatat tctttttttg gatgagccaa cccgtgggat
    18421 agatgttggg gcaaaatatg agatctacac gattatcgaa gaaatggcag caaatggcaa
    18481 agctgtctgt cttatctctt ctgaacttcc tgaaatatta gggatgtgtg atcgaatcta
    18541 tacgatgaac gaaggacgta ttacaggaga agtctcgcga gaagatgcag atcaggaagt
    18601 cttgatgaaa ttaatgacga aagaagaggt gggttaagtt ggaacaggca aaaaatctta
    18661 cggataaaaa agagaaaaaa ctaaatttga aaagtagagc agctgatctt tttggtcgct
    18721 acagcatggt gatcatttta gcgggactac ttattgcgtt tcaattgatg acggatggta
    18781 ttttctggcg tccattgaat ataactaacc ttgttctgca aaatagccat atattagtac
    18841 tagctgcagg gatgttatta gttgtgctct taggtcatgt ggatttgtcg gtagggtcag
    18901 tgatggcgtt tgttggtgca attgcgggca tgatgatggt caatcgccaa atgaacccgt
    18961 ggatgacagt tatcgcatgt cttgcagtag gcgggctgat cggcgcatgg caaggttttt
    19021 gggtagctta tgcagggata ccggcgttta tcgtgacttt agcaggattg ctgatgtttc
    19081 gtggactgac acaagttgtt ctaggaggcc aatctctagc accttttcca gctgttttcc
    19141 aaaaaatgtc tacgggttat ttaccagact taagcgaagg aagcagcctt cacttcttga
    19201 cattgatttt aggaatgatc atcacgatcg tgttagttgt tggtcaatgg cgaacaagaa
    19261 atcgtcggaa aaatcattta tttgaagtgg aatctccaac agctttcttg gtaaaggcgg
    19321 tatttacagc agtcgttgta ctaggaataa cttatatctt tgcctcttat caagggtatc
    19381 cgattatttt gatcattctt ggtttgatcg ttgcagcata tgggtttttg acaaataaaa
    19441 cagtagcagg tcgacagatt tatgcgacgg gcggaaatcg aaaagcggct gaactatcag
    19501 ggatcaaaac aaagcgaatc acgttctggg tattcgtcaa catgggagtc atggcagctt
    19561 tagctggatt agttttagct gctcgattga atgcggcaac acctcaagca ggtacatcga
    19621 tggaactgga tgcaatggcg gcagtatatt ttggtggtgc ttcgacttcc ggcggcattg
    19681 gaacaatcat gggtgcgatt gtcggtggct tggtcatggg tgtattgaac aatggcatgt
    19741 cgattttagg cgtcggggta gactggcagc aggcaatcaa aggattgatc ttgcttttag
    19801 ccgttgtgct agacttgtat aataagaaaa aacgaacaac gtaggagaac gccggttatt
    19861 cagaaaaaag actgtgacaa gacatttgtc acagtctttt tttcgctaaa agcgaacatt
    19921 cattgtttcg tttgtataga ctaaaaataa tatatgtttt tttaccattc gtataaattc
    19981 tctttttgct cttgattttt ttgcttaatt gagttaaatt acatagggcg ctaaaaaaag
    20041 ccttgattaa attaataatg ttcgtgtttt gctggatgtg aacatcagga ggataatgta
    20101 atgaaagtat ttgattacga agatattcaa ttaattccta ataaatgtat cgtaaatagc
    20161 cgttctgaat gtgatacaac ggtcacatta ggtaaacata cattcaaaat gccagtagtg
    20221 cctgccaaca tgcagacgat tattgatgaa acaatcgcag aattcttagc ggaaaatggt
    20281 tatttctata tcatgcatcg gtttgatgaa gcagctcgaa ttccgtttat caaaaaaatg
    20341 aaaaagcgtg gattgattac ctcgatcagt gttggcgtca agaaagagga atattccttt
    20401 atagagaagt tggcagaaga atcgctaaat cctgattata tcactatcga catcgcgcat
    20461 ggacatgcta actcagttat tgatatgatt cagcatatta aaaaatattt gcctgaaact
    20521 tttgtgattg ccggtaatgt tggtacaccg gaagccgtcc gtgaattgga aaatgcgggc
    20581 gcagatgcaa caaaagtcgg gattggtcca ggaaaagtat gtattacaaa aatcaagaca
    20641 ggctttggga ctggaggctg gcaactggca gctcttagat ggtgtgcgaa agcagctcgc
    20701 aagccgatca tcgctgacgg tgggatcaga acacatggcg atattgcaaa atctgtccgt
    20761 tttggtgcga caatggtcat gattggttca ttgtttgccg gtcacgagga atctcctggc
    20821 gaaacaaaag tcgaaaatgg aatcgtgtat aaagaatact ttggttctgc ttcagaattc
    20881 caaaaaggcg aaaaacgaaa tgttgaagga aagaaaatct ggatccaaca caaaggatct
    20941 ttgaaagata cactagtgga aatgcaacaa gaccttcaat catctatttc ttatgctgga
    21001 ggaagagatc tggaagccat tcgaaaagtt gactatgtca tcgtgaaaaa ttcgattttt
    21061 aatggagaca caatctaaaa aataaataaa atattaggat gttgtgaaaa agttgtcctg
    21121 cattaagtaa taagaacagc caaacaaaaa tagttcttta tatgttttgt ttggctggaa
    21181 ttattatcgc agatacaagc ttttgaacat cattattcag aataagggac tgtgataaga
    21241 tttttgtcac agtcccttat gttttgcaaa aaaagttttt cttttttctc gtaaattcgg
    21301 taaaatcaaa agaaaaaata gacaatagaa aagagaagct tatgtgggaa aaagtagaga
    21361 aaaaacaagg accgatttat cgtcaaatta tggaacagat tatgagagaa atcgaaaacg
    21421 gaaggatcaa tcctggaagt caacttgaat cagaacgaaa actagggcaa ctttttggtg
    21481 tgaatcgttc caccgttgtc catgcattgg aagagcttag ggagcttggg gtactaacga
    21541 gcaaacgtgg cagcggatgg tttgttaatc agactgaatg ggggaaattc gctgttccaa
    21601 gaatcgattg gcggcaaatg atctctcctc gttatgagca gacggactgg tatgagcaaa
    21661 aaataagaga agcaaagcag atccaaaaaa catcgtttct tgatttatac gctagcgaga
    21721 tgccagatga gcttttgccg aattttgagt ttccttctta ttctcttgaa gaaatcttag
    21781 cggaagaaaa agaaatggat cttttaggct atactccgtt acgtaacaaa atcaaacggt
    21841 atttggaaag taaatttgaa ttggcatttt cccccaacca gcttcttgtg actggtggcg
    21901 gtcagcaagc aatctttttg attttacaaa cgattctttc agctggagag gggatagctg
    21961 tagagtcacc ttcttttttt taccgattac ctcttttcaa agcttcagga acgcgtttat
    22021 ttgggatacc gatggatgaa gaagggatcg acttagaaaa actgaaagaa tcgatccata
    22081 aaaataaatt gaaagcagtg cttatcaacc cgaattttca aaaccctaca ggaaaagtga
    22141 tgagtcaaaa acgacgagaa aagctagtag aattgtgtcg acagtatcgg cttccaatta
    22201 ttgaagacga tgtatttacg gatctttcat ttctaaacca agaagaagtc cttcctatac
    22261 gaacgattga tccggataat gtgttatacg tcggttcgtt atctagggtt ttaggaaaaa
    22321 caacgaaaat cggttgggtg ataggacctg agacttttat ctcgcaacta gctcaagcgc
    22381 aagaaatgat ggagttttcg atgaatatcc ttacacaaat cacagtggct aatgtattta
    22441 atgaagcaat tgatttgaaa atgcaagatg taagaaaaaa gttgaaagaa aaaagccgac
    22501 tgataagcgc ttggggagat tcacaatcgt ttttcaagct tcattcaatt ttaggtggtt
    22561 actatgcctg gattacttgg gatgggaaaa aactagaccc cgaactagca gaaaaagtag
    22621 tagatatggg tcttggaatt gcaccaagtc tactttttgg gcaagatata aatggaatca
    22681 gaatcaatta cagtaggctg gatgaaaacc aaataccgtt gttttattca aagatgaaaa
    22741 ttctagaaaa gtggttaaca gaataatttc acaaagtaaa tagattaaag aatataacag
    22801 cttggattta aatttcaagc tgtttctttt ttttcggaaa aagaatacga tataaagaaa
    22861 ctaagaaaaa accattattt gtacgtccgt tcacgatgtg taaataaaat aaatgtttgc
    22921 aacaaaactg tataacacca acgtttttaa atagaaacag atggttgtaa gtttgattag
    22981 aaaatagaaa aaatgcagat gaaatataaa acgctttcct tattgtgatt ttattcacta
    23041 atgctgttaa tatgcggatt ggcataattt cctttcatcg tgaaaatttg tgcttttagc
    23101 ttgtatttct cataaatcca tgataattta ttaatgaaga cggttaaata gtgatacagt
    23161 tttattcact gtttcaatcg cagaatgaag agattttctt atagatgaga gaggaatgtg
    23221 tgacaaatgg caaacaaaaa agcaccgatc gacttccaag cactgctaga tgaagtcaat
    23281 gcagatttcc cagtatatca agcacttgat caagatggaa acgtagttaa caaagattta
    23341 gtacctgatt tatccgatga tgaactcgtt gaattaatga ctcatatggt atggtctcgt
    23401 gtattagacc aacgttcaac agcgctgaac cgccaaggac gcctaggctt ttttgcacca
    23461 actgctggac aagaggcaag tcagctggct agtgcttacg catttgataa agaagatgtt
    23521 cttcttccag gttatcggga tgtacctcaa ctagtaaaac atggtttacc tttatctcaa
    23581 gcttttttat ggtctcgagg acatgctgca ggtaattctt atccggaaga attgaaagct
    23641 ctaccaccac aaattatcat cggtgcacaa tatgtacaag cagccggtgt tgcattaggt
    23701 ttgaagaaac gaaacaagaa aaatgtcgtc ttcacttata ctggtgatgg cggttcatca
    23761 caaggtgatt tttacgaagc aatcaatttc gctggcgctt accatgcaaa tgccgtattt
    23821 tatatccaaa acaacgggta tgcgatctct acacctcgtg aaaaacaaac tgcagcaaaa
    23881 acacttgcac aaaaagctgt cgcagctggg attcctggta ttcaagtcga tggaatggat
    23941 ccgcttgcag tatatactgt atctaaaatg gcacgggaat gggcgatcag tggaaatgga
    24001 ccagtcctaa tcgaaacact gacttatcgt tatggaccac atacactatc tggtgacgat
    24061 ccaacccgct accgtacaaa agacacagat gacgattggc agaaaaaaga tccattgatc
    24121 cgcttccgta aatatttgac ggaaaaagga ctttggtctg aagaaaaaga agaacaagtc
    24181 atcgaagcaa ctaaagaaga aatcaaagca gctattgctg aagccgacaa agtaccaaaa
    24241 caaaaggttt cagatttctt gaaaaacatg ttcgaagtat ctccacaatc aatcaaagaa
    24301 caaattgcaa tttatgaagc gaaggagtcg aaataatcat ggcacaaaaa acaatgatcc
    24361 aagcaatcac agatgcctta gcacttgaac tagaaaatga cgagaatgtc gttgtttttg
    24421 gtgaagatgt tgggaaaaac ggaggagttt tccgggcaac tgaaggattg caggaaaagt
    24481 tcggagaaga ccgagtattc gatactcctt tagctgaatc tggaatcgca ggattgagtt
    24541 tcggtcttgc gttagaagga tttcgtccag ttcctgaaat ccaattcttt ggttttattt
    24601 tcgaagcaat ggacgaagta gtagctcaaa tggctcgtac aagatatcga atgagcggca
    24661 ctagaaatct acccatcaca attcgttcgc catttggtgg cggtgttcat acaccagaat
    24721 tgcattcgga taatttagaa ggtttgatcg ctcagtctcc tggtattcga gtagtgattc
    24781 cttccaaccc ttatgatgca aaaggactat tgatttcttc tattagaagt aacgatccgg
    24841 ttgttttcct agaacacatg aaactatatc gctcattccg agaagaagtg ccggatgaag
    24901 cttacgaagt tcctttggat aaagcagcag ttactcgtga gggaacagat gtatctatta
    24961 tcacatacgg agcaatggtg cgtgaagcaa tcaaagcagc agacaatctt gcaaaagaaa
    25021 atatatctgt tgaaatcatt gatcttcgta cagttgcgcc attagatgta gaaacgatca
    25081 ttcaatccgt ggaaaaaact ggtcgcgtag ttgttgtcca agaagcacaa cgacaagcgg
    25141 gcgttgccgc gcaagtagtt tctgagatct cagaacgcgc tatcctttca ttagaagccc
    25201 caatcggacg agtttctgca cctgatacag tcttcccatt tggacaagca gaaaatgtct
    25261 ggttgccaaa tgcaaaagat atcgaagaca aagtaaaaga aatcgcggaa ttttaagaaa
    25321 agaagggacg aaacaaagtc atggcttatc aatttaaatt gccggatatc ggtgaaggaa
    25381 tcgcagaagg tgaaatcgtc aaatggttcg tcaaaccagg agatacgatc aatgaagatg
    25441 atacattact agaagttcaa aatgacaaat cagtagaaga aatcccgtca cctgttacag
    25501 gaacggttaa aaatgtgatt gttccagaag gtaccgtagc aaatgttggg gatgtattag
    25561 tagaaatcga tgcaccagga cacgaagaca atgaagggga cagtggtgta gccgcagaat
    25621 cccaaactcc agctaaaccg gctgctgaac ccactgttga tacagaatca gctggatcgt
    25681 caagcgaagg agtcttccaa tttaagttac cggatatcgg tgaaggaatc gcagaaggtg
    25741 aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat acattactag
    25801 aagttcaaaa tgacaaatca gtagaagaaa ttccgtcacc tgtcacaggg acagttaaaa
    25861 acgtgattgt tccagaaggc actgtagcaa atgttgggga tgtattagta gaaatcgatg
    25921 ctcctggaca caatagtgca ccgtcaacta gtgcgccatc agctgaagca ccaaaagaaa
    25981 aagtggaaac atcaggttca gcaagtgtcg ttgaagctgc cgatccaaat aaacgtgtat
    26041 tggctatgcc atctgttcgt caatttgccc gtgaaaaaga tgtagatatc tcacaagtaa
    26101 ctgcaactgg aaaaggtggc agagtaacaa aagaggacat cgaaaacttc ttggcaggtg
    26161 gtccaagcag tgcaccagcg aaatcagaag cgccagaagc ggcagcaccg aaagaagcag
    26221 ctcctgcagc agaaagcaaa ccagctgcac cagcaaaacc attcaagtcc aacttaggag
    26281 acttggaaga acgtgtagca atgacgccaa cacgtaaagc tatcgctaaa gcaatggtca
    26341 acagtaagca tacagctcca cacgtgacat tgcatgatga agtagaagtg tctaaactat
    26401 gggataatcg gaaacgattt aaagaagttg ctgctgcaaa tggtacgaaa cttactttct
    26461 tgccatatgt cgtgaaagct ttaacagcga ctgttaagaa atacccagta ttgaatgcat
    26521 ctatcgacga tgcaaaccaa gaaatcgttt ataaacatta ctacaatatc ggtattgcaa
    26581 ctgatacgga tcacggacta tatgtaccaa atgtgaaaga tgcagaccgc aaaggaatgt
    26641 ttgctattgc agacgaaatc aatgagaaag caaaattagc tcatgacggc aaactatctg
    26701 cagaagatat gcgcaatgga acaattacga tcagcaatat cggatctgta ggtggcggtt
    26761 ggtttacacc agtcatcaac tatccagaag tagctatttt aggtgtagga acgattgcac
    26821 aacaaccaat cgtaaatgct gaaggcgaaa tcgttgtcgg tcgtgtgatg aaactgtcat
    26881 taagtttcga tcaccgtatc gtagatggag caactgcgca acaagcaatg aataatatca
    26941 aacgtctttt agctgatcca gagctattaa tgatggaagg atgataaaca aatggtagtt
    27001 ggagattttg ctgtagaatt agatacagtt gtaattggtg cgggacctgg tggctatgta
    27061 gctgccatcc gcgctgctga aatgggacaa aaagtagcaa tcattgaaag agagtatatt
    27121 ggcggtgttt gtttgaatgt cggctgtatt ccttcaaaag cactgatagc tgcaggacac
    27181 cattatcaag agtcactaga ttctgaaatg tttggagtaa ctagtgaaaa cgtcaaacta
    27241 gattttgcaa aaacacaaga atggaaagaa aacaaagttg tccatacgtt gacttctggt
    27301 gttggtttcc tattaaaaaa acataaggtt gaaacaatcg aaggagaagc gttcttcgta
    27361 gatgatcaca cactacgcgt cattcatcca gattcagctc aaacttattc gttcaataac
    27421 gcaatcatcg caacaggttc ccgtccaatc gagattccag gatttaaatt cggaggacgt
    27481 gtgttagatt ctactggcgg attagcattg aaagaagtac ctaagaaatt tgtgatcatc
    27541 ggtggcggtg tgattggtgc cgaattgggc ggagcgtacg caaacttagg agcagaagta
    27601 acgattcttg aaggctcacc acaaatttta ccaacttacg aaaaagatct agtaaaatta
    27661 gtagaagatg atttcaagaa aaaaggcgta acagtcgtaa cgaatgcaat ggcgaaagaa
    27721 gcagtcgata acggtgacag cgtgacagtg aaatatgcag tggacgggaa agaagaatcc
    27781 gttactgctg attatgtgat ggtcactgta ggtcgtcgtc cgaatacaga tgatatgggc
    27841 ttagaacaag cgggtgtcga agtaggagaa cgaggattga tcacggtgga taaacaagga
    27901 cgtacgaacg tacctaacat ctatgccatc ggtgatatcg ttccaggggc ggcattagca
    27961 cacaaagcca gttatgaagc aaaaatcgct gctgaagcaa tctctggcaa aaaagtagct
    28021 gtagattata aagcaatgcc ggcagtagcc ttcactgatc ctgaattggc ttctgtaggt
    28081 atgacgatca aagaagcaaa agatgctggt atcgaagcaa aagcatataa attcccattt
    28141 tcagggaacg gtcgggcatt atcgcttggt aaaacagaag gattcatccg tctagttacg
    28201 acgatcgaag ataacgtgct tattggcgcg caaatcggtg gagtcggcgc aagtgatatg
    28261 gtctctgaat tagctttagc aatcgaatca ggaatgaatg ctgaagatat cgccttgacg
    28321 attcatcctc atccatctct tggcgaaatc gtcatggatg cttcggaatt agcattaggt
    28381 ttaccaatcc atatttgatt tgaataatac aggcgaaacg caactgaaaa atcagttgcg
    28441 ttttgttttt tagaattatc aataatatta ttatgtaaac ttaaaaatat gacaacttct
    28501 tggtttatta ccacttccta tgctacactg aagaggaata tgaaatagga gagttgaaga
    28561 tgcaagaata tcagtatcca cttgatttag attggacaac agacgaaatg gtcattgtaa
    28621 tgaagatgtg ggaagatctg gaacgtgcga atgaaaaagg gataaacaat gaagcttttt
    28681 tgaagactta tcagcagttt aaaactgttg tcaaatccat tggtgaggaa aaacgattgg
    28741 gacgggaatt tgaaaaagct tccggttatt ctttatatcg tacagtgcag caagcaaaaa
    28801 aaaatccaaa caaaactttg agaatgaacg ggtgagataa atggaacaga tgattgatca
    28861 tatcaagtcc tggcttgaag aagctggtga gaaaatcaaa tcttctcaac aaacattaac
    28921 aatctcgcaa aaatcaaatc gaacggatct agtaacaaat gtcgatcgaa acatccagaa
    28981 ttttctaatc gaaaaaatcc attcctttga tccagaggcg aaaatattgg cagaagaaga
    29041 agggtgtaat caattatcgg atctcgatgg aagagtcttt atcatcgatc ctattgatgg
    29101 caccttgaat tttgtgattg aaggggaaaa tttttgtatt atgctcgctg tttatgaatc
    29161 cggcgttgga aagcttggtt tcatttatga cgtgatgaga aatgaacttt tttggggtgg
    29221 aaaaggaata ggcgtgtata aaaatgatga gccattgcct agaccagcaa ataaatcctt
    29281 atcggaaggc ttacttggtg tgaatagtta tcttttcggt cataataaat atggaatccg
    29341 agaaattgcg gaaagcagca tggggacacg tatgtacgga tgcgctggtc ttgaactagt
    29401 ggcaatcttg aaaggaaacc acattgctta tatttcaaac cttagccctt gggattatgc
    29461 agcgggaaat gtcttattgg aagagtttgg tatggtaaca agcggtcttt ttggtactcc
    29521 tttgacattt gaaaaaaggg aatactattt agcagggact tcttgtgcat atgcagaaat
    29581 cgcagacctt ttgaaaaaag aaggctgaaa aaaaggtaga aatcattcac aaaaatgata
    29641 aaaacgagca aaagattaat tttctcgaaa aaatgaaaac attttactat atttcatgaa
    29701 aaaagtctgc tataattagc agtcgagtaa aatttgccct gtcataaaga accaataaaa
    29761 aaggagttag ttacattgaa ttatagaaac gatatccgta acgtcgcaat tattgcccac
    29821 gttgaccacg gaaaaacaac attagttgat gaactattaa aacaatcaga tacattagac
    29881 gcacatacgc aattgcaaga acgtgcgatg gactcaaatg cgctagaaaa agaacgtggt
    29941 atcacgattt tagccaaaaa cacagctgta gactataaag gtattcgcgt aaacatcatg
    30001 gatactccag gacacgcgga cttcggtggc gaagtagaac ggatcatgaa aatggttgac
    30061 ggcgttgtgt tggtcgtaga tgcgtatgaa ggaacaatgc ctcaaacacg ttttgtattg
    30121 aaaaaagcac tggaacaaca tatcacacca atcgttgtcg taaacaagat tgacaaacct
    30181 tctgctcgtc ctgagcatgt cgtagatgaa gttcttgaac tattcatcga attaggagca
    30241 gatgatgatc agttagactt tcctgtgatt tatgcatctg cactaaatgg tacgtcaagc
    30301 ttgtcagatg atccagcaga tcaagaacca accatggcac ctatttttga tacaattatt
    30361 gaaaaaatcc ctgctcctgt cgataacagc gacgaaccat tacaattcca agtttcatta
    30421 cttgactaca acgattatgt aggtcgtatc ggaatcggac gtgtatttag aggaacaatc
    30481 aaagtaggag accaagtagc attaattaaa ttagacggaa cagttaagaa attccgtgta
    30541 acgaaattat ttggtttctt tggactaaaa cgtctagaga ttcaagaagc aaaagctgga
    30601 gacttgattg ctgtctcagg tatggaagat atctttgtcg gtgaaacagt tactcctgtg
    30661 gatcatcaag atgcgttacc tatccttcac attgacgaac caacattgca aatgactttc
    30721 ttagttaata attcaccatt tgccggtcga gaaggtaaat tcgtaaccgc tcgtaagatt
    30781 gaagaacgtt tgatggcaga acttcaaaca gatgtatcct tacgagttga accaacaaac
    30841 tctccagatg catggacagt ttctggtcgt ggagaattgc atttgtctat cttgattgaa
    30901 aacatgcgcc gtgagggata cgaattgcaa gtatctcgtc cagaagttat tgaaaaagaa
    30961 atcgacggtg taaaatgcga accgtttgaa cgcgtgcaaa tcgatacacc agaagaatat
    31021 atgggtagtg ttatcgagtc cttgagctta cgtaaaggtg aaatgcaaga tatggtccat
    31081 acaggaaacg gacaaatccg tttgacattc ttaacacctg cccgcggttt gattggttat
    31141 tcaactgaat tcctttcaat gacacgtggt tacggaatca tgaaccatac ttttgaccaa
    31201 tatctgccaa tgcttcctgg tcaaattggc ggacgtcacc aaggggcgct tgtttcgatc
    31261 gatacaggaa aagcaacgac ttattctatc atgagtatcg aagaacgcgg tacagtcttc
    31321 gtagaaccag gtacagaagt atatgaagga atgattatcg gggaaaacag ccgtgataat
    31381 gatttgactg tcaacatcac gaaagcaaaa caaatgacaa acgtacggtc tgctactaaa
    31441 gaccaaacat ctgtcatcaa aaaaccaaaa caattgacgt tggaagaatc tttggaattc
    31501 ttgaatgatg atgaatattg cgaagtaaca ccagaaagca ttcgcttgag aaaacaaatt
    31561 ttagagaaaa atgctcgtga aaaagcatct aaaaagaaaa aataatctgt aaaaggatga
    31621 agagagaaac cagcttgtta taaaaaatag ctggtttctt tttttgtcca actcttttct
    31681 gtggagaagt catctttgtc aatggacata aattcagaaa atacgccgaa agacgtttaa
    31741 aagactttta aaaaacttat ttctagagag tttttgtttc ataataagat aatgtttcac
    31801 gaaaggagat caacttgtcc ttcgaagaaa atcgaagtaa aatggcaaga gaaaggagcg
    31861 ggaagatgaa cgacttatta aaacaatact ttggctatga tgagtttaga cctggtcaaa
    31921 aagaaattat ccagaaagtc attgatcagg aaaatgtatt aggaatcatg cctaccggaa
    31981 gtgggaaatc aatctgttac cagcttccag ctttactatt agatgggctt actgtggttg
    32041 tgtccccttt aatctctttg atgaaggatc aggtggacgc ggcgaatcaa ttaggtatac
    32101 ccgccacttt tatcaatagc tcgttagatg gttacgaaac agctagacgc ttccaagaaa
    32161 tcgatagaca gcagtaccgt ttactttata tcgctcctga acgttttatc atgccggatt
    32221 ttatccaagc aatgaagcga tggaacgtcc gtatgatcgc tattgacgag gcacattgta
    32281 tctctcagtg ggggcatgat ttccgcccaa gttatttaca gatggcgaac cagctagatc
    32341 aactgccaaa tcgtcctgta attgttgctt tgacagctac agcgactgtg caagtagctg
    32401 ctgacataaa aagactttta aaaattccag aaaacaacca tattcagact ggttttgaac
    32461 gagaaaatct acgatttcaa gtaattaaag atcaaaaaaa agaacaatat ctaatcgaat
    32521 atttaaagat caacaaaaat caatctggta tcatttatgc agcaacgaga aaagaagtag
    32581 accgtctcta tcatttgctt aaaaaattcg atttttccgt gggtagatat catggtggtc
    32641 taaatgagaa tgagcgaaca gaaatgcaag aagccttttt atacgatcgc ttgcagctga
    32701 ttgtagcgac aaatgcattt ggtatgggta taaataaaag caatgtccgt tttgttatcc
    32761 attatcaaat tcctggctct ctagaagcat actatcagga agctggtcgg gcgggaagag
    32821 atggtctttc cagtgaagcc atcctgcttt tctctcctca agatatccaa gtccaaaagt
    32881 tttttatcca gcagtcccaa agagaagaag ggcaaaagca aaaagaatat gaaaaattaa
    32941 aagcgatgac agaatacgtg tatattgagt cctgtcttca gcaatacatc ctaaactatt
    33001 ttggcgagac aagcagtccg tgcaaccgtt gtggtaactg tttagatgac agagaaatag
    33061 tagaagtaac gacagaagca cagatggttc tttcttgctt aaaacgtatg ggtgagaact
    33121 atggtaaaca aatgctgatg aaagtcttag caggatcgaa agaacaaaag ctgcaagctt
    33181 taggttttgg acatttatct acctatggac tgatgaaaaa ccaatcgcaa aaagaaacga
    33241 tgcagttaat tgaatatttg atatcgaatg gctatcttct gaccatcaat ggtgagtatc
    33301 ctgttttgaa agtaacagaa agagggatac aagtattgaa aggacaagaa tctgtttatc
    33361 gaaaggaacc caagaaagtc cagcagctat cggatgaaga aaccgataca ctatttgaag
    33421 tgctgcggga actgcgaacg gatctagcat cagaagcagg cgttcccccg tatgtcgtat
    33481 tttcagattc cacattgaaa gaaatgagcc gaatacgtcc atcaagcaga ttggagatgc
    33541 ttcagataaa aggagttggc caaagcaagc ttgataaata tggcgaagcc tttttatctc
    33601 ggataaaaaa tgcggactct aacgttctgg ataagtagaa agaacgaata cgtaaaaatg
    33661 aataaaaaca actggttaaa tctgtttttt ttgctcctta tgaaaatcaa atatgttata
    33721 attaaggaat tagaatggga ggtaagcaca gatggattct ttgtcgaaaa aacttgcgat
    33781 ccaagtatta aatgatgaag cagaacggat caaacgatta atccgaaatc aaaacaacag
    33841 tctctgtatc tcacaatgca aagccttcga agaagtggta gatacacaga tgtacggctt
    33901 ttcccggcaa gtcggttttg ctgtccgtat aggagtaatc ggaaagaacg agggccagct
    33961 gatgttaagt gagctagaac gagaattgaa tcagttatat agtgaggttt accaagaaat
    34021 ttatgataaa aaagaagttg gcaaggagga atagacttgc ctaagaaagt gaaaaaaagg
    34081 catctattag actatagcat cttgattccc tatttaattt tatgtgtatt gggattgatc
    34141 atggtatata gttctacgtc gtatctatta ttagaaaacg gacaaaatcc gtccgcatca
    34201 gtcattaacc agagtatttt ttgggtgctt agcttgattg ctatcgcact actttataaa
    34261 atgaaaacag atgttttgaa aaaccagcgt ttgatcatgg cagccatcgc agttttgacg
    34321 attttgctgc tgattgttgt gttttttggt aaggagatca atggggcgaa aggttggctg
    34381 caaattgcag gattctcgat ccaacctgcc gaatacctaa aaatcatttc tatctggtac
    34441 ctgtctctga ctttatctaa aagacaaaac agtgtacaaa aagattttct tgggactgtg
    34501 aaacgtccac ttgcaatggt tatcggactt actgcattag tcgctattct tcccgatttt
    34561 ggtaatgcgg cagttatctt tttgattatt ttggttctgc ttttagccag cggagtcaat
    34621 tatgtgtata ctttgattgt aggagtagga ggcttttgtc taagtacttt tacgatctgg
    34681 ctgatcaata tcacaaacgg aaagatctta cccggacgtt tacagtatat ctataatcga
    34741 tttgctattt accaaaatcc tttctcagac gaattaaata aagggcatca actagtcaac
    34801 ggctactatg ccatgttcaa cggaggacta tttggcaggg gattaggtaa cagtatccag
    34861 aaaaaaggat ttttacaaga agcacaaacc gattttattt atgcaatcgt agtggaagaa
    34921 ctgggagtga ttatggggat tttgatatta gcgttgctat tctttatgat tgtgcgcatt
    34981 gtccttgtag gtatccgttc aaaagatccg ttcaattcgc ttttgtgcat tgggatcggc
    35041 gcaatgtttt tgatccaagt atttgtcaat cttggaggaa ttactggcgt tattcctttg
    35101 acagggatca cgttcccatt tttaagtcaa ggaggctcca gtttgctgat gctttcgatt
    35161 tgtgtcggct ttgtcctcaa catcagtgcg gatgaaaaac gcaagagctt aggtttataa
    35221 taaaaaaaga tgtaggagtg gttcgatgaa aaaagtttta gttgctaaca ggggtgaaat
    35281 cgctgtacgt gtatttcgtg cttgtacaga attaggaatt aaaactgttg gaatctatgc
    35341 agaagaagat gaatattccg tacaccgttt caaagcggat gaagcatatt tagtaggaca
    35401 aggaaaaaaa ccgattgatg cgtatctaga tattgaaggg atcatttcta tcgcgaaaga
    35461 atgcggagct gacgctattc atccgggtta cggtcttttg tcggaaaact tgaattttgc
    35521 gcaacggtgc gaagaagaag gcatcatttt tgttggaccc aaactgcatc atttagatat
    35581 ttttggagat aaaatcaaag caaaagctgc agctatcgaa gcggggattg cttcgattcc
    35641 aggcacagat ggaccaatcg cttctataga cgatgccttg gagtttgcga aacaatacgg
    35701 ttatccgatt atgatcaagg cagctctagg cggcggcgga cgcggcatgc gtgtagctca
    35761 cgatgaaaag agtgcaagag agggctacga acgtgcgaaa agcgaagcaa aagcagcctt
    35821 tggtagtgat gaagtgtatg ttgaaaaata catcgctaat cctaagcata tcgaagtaca
    35881 gattctcggg gatacgcatg gaaacgtcat ccatcttttt gaaagagatt gttctgttca
    35941 gcgccgtcac caaaaagtag tagaagtagc gccttgtgtc tctatgaatg aacagcaacg
    36001 tcaaaaaatc tgccaggcag ctgtccaatt gatgaaatat gtcgggtatg tcaacgcagg
    36061 tacagtagaa tttcttgttg aaggagatga tttttacttt atcgaagtca accctcgcgt
    36121 ccaagtggaa catacgatca cagaaatgat tacagatatt gatatcgtaa caacgcaact
    36181 gttgattgct caaggtttgg atctgcataa agaaatcgga ttgcctcagc aagaaggaat
    36241 taaattaaat ggttcagcca ttcaatgccg aatcactaca gaagacccgt tgaataactt
    36301 tttaccagac acagggaaaa tcgatacgta tcgttcacct ggtggttttg gcgtacgtct
    36361 ggatgtcgga aatgcttacg cgggctatgt agtgacaccg tattttgatt cattgctagt
    36421 aaaagtatgt acgcatggtg ctacctttga aacagccatt cagaaaatgg aacgttgctt
    36481 gaaagaattt agaatccgtg gagtgaaaac aaacattcca tttatgctca atgtcatcac
    36541 acatcctgaa ttccaatcag gaaatgcaaa aacaaccttc attgatagta cagcaacatt
    36601 atttgaattt cctcgactac gagaccgcgg aaacaaaacg atgaaatata tcggtgaaat
    36661 tactgtgaat ggttttccag gtatcgaaag tggcgaaaaa ccattttatg aagaaccacg
    36721 tatgcctaaa gatctaatca caagaactga ttacgtaacg gctaagaatg tattagatgc
    36781 gaagggtgcc gatgctttgg tcgaatggat caaaggacag gaaaatttgt tgttgacgga
    36841 tacgacattt agagacgccc atcaaagtct gctagcaact cgtgttcgga caaaggattt
    36901 caagcagatt gcacgtttga ctggagaagg tcttcctgaa ctcttttcaa gtgagatgtg
    36961 ggggggagca acatttgatg tggcttaccg ctttttgaat gaagatcctt ggcaacggtt
    37021 gagaaaaatt cgttctttaa tgcctaatac gttgctgcaa atgcttttca gaggttcaaa
    37081 tgccgttggg tattctaatt atcctgacaa tgtattggtg gaatttgtca aagaagcagc
    37141 agcgcaagga atcgatgtat ttcgaatctt tgatagtttg aactggactc cacaaatgga
    37201 aaaaagtatc caagctgtac gggatactgg taaaatcgca gaggcagcta tttgctatac
    37261 tggcgatatc aacgatccaa gtcgggcaaa atacaatgtt cagtattata aagacatggc
    37321 aaaggaacta gaacaactgg gtgcgcatat cattgcaatc aaagatatgg ctgggctgct
    37381 taaaccgcag gcagcttatc gtttgattag tgaattgaaa gaaacgacag atctgccaat
    37441 ccatctgcat actcatgata ccagcgggaa tggaatcatc acttattctg ctgcttcaaa
    37501 agcaggagta gacatcgttg acgtggcaat gagtgcaatg agtgggaata ctagtcagcc
    37561 tagcatgagc agcttgtatt atgcactggt caatggtcca cggctgccag aaatcacgat
    37621 tgaaaatgca cagaaattaa accattattg ggaagatgtg cgcatgtatt acaaaccgtt
    37681 tgaaaacggc ttgaatgctc ctgaaacaga agtatatatg catgaaatgc caggaggaca
    37741 gtattcgaat ctacagcaac aggcaaaagc tgtcggatta ggtcatcgct gggatgaaat
    37801 caaacaaatg tatcatactg tcaacttgat gtttggcgat atcgttaaag taactccatc
    37861 atctaaagta gttggagata tggcactatt tatggtccaa aatgatctta cagaagaaga
    37921 tatttatgaa aaaggcgaaa cactaagttt ccctgaatct gttgtgactt tcttccaagg
    37981 agaactaggg caacccgttg gcggatttcc taaaaaattg caaaagatta ttcttaaagg
    38041 cagacctgca ttgaatgaac gtccaggtct tttggctgaa tcagtcgact tcaacgaagt
    38101 aaaaaaagaa ttggcagaaa aaattggcta tgaaccaaaa caagacgaag tattaagcta
    38161 tttgatgtat ccgcaagtct ttttggacta tcaaaaagca tataatcaat ttggcgatgt
    38221 tacattactg gacacaccaa catttttcca aggtatacgt ttgggtgaaa caatcaatgt
    38281 acaaatcgaa cgcggaaaaa cgcttatcat tcgtttagat gaaatcggag aacccgatat
    38341 cgaagggaat cgcgtactgt tcttcaattt gaatggacaa agacgtgaaa tcactatcaa
    38401 agataattcg atcatcagta cggtccaaac ccgattgaaa gctgaaccga caaaccgaga
    38461 acaaattgga gcaacgatgt ctggttctgt tttggatgta ttagtgaaaa aaggcgataa
    38521 cgttaaaaaa ggtgatacgt tgatgattac tgaagcgatg aaaatggaaa cggccatcga
    38581 agcacgtttt gacggagaag tcgcacatgt atatgtgtct tcaggagaca ctatttcgtc
    38641 tggtgacctt ttgattgaag tgactgagaa ataataagcg aaaggtgggg aaacatgaaa
    38701 agactgggtt tgttttggct gattctgcta ttagttattg tggcattcta catggaacca
    38761 gtcttgtttc cgccaaaact tgaagtgaag gaaaactctg atcaacaaat atcaaaccaa
    38821 atgacaacgt ggaaatacca ggagcttaaa gcaactggtt tttctactta tatcggaaaa
    38881 ccgatcgatc agctggaaaa ggattttggc caagcctatg atcgattaac tagcggattt
    38941 ggttttgaaa caagatatta tgaaaataaa gctagtcatc tcacatttga agcgaacatc
    39001 caagacagca gagtcacaac tgttaaagta ctgcaaacag ggcctgctga tatcgcgccg
    39061 ttttatttag ggatgacgat gcaagatctt acgaaaatca ctactatcta tactaatttc
    39121 acttttgagt accatgatac agaagttgga atcgaactaa tggaagagga tatgaattat
    39181 cgtccgctaa ttgcttttga taacgacact tttgctattt tattttttga tcaaacaaca
    39241 aacggcctat tttcagttgt gtatttagat aaagatagtc ttttgaaact attgccatat
    39301 caagtgtttg gtgaaggcct tccacattat caagcagata aatcggctga ttgggaatca
    39361 atcaatcgcg ttaaagaacg aaaaagtacg actttattaa atatgcttag aagagaagac
    39421 gaacttccca tatacaatca aacacttgcc tttcaaaacc acagtcagct gctactacat
    39481 gattttttga caaggcctga ggaaatattg tcagaggaac gtatggctga ttggcaaaaa
    39541 tcgctaggca gtgctcaaac gacagtgaaa ttctcactga cgaatgatga attggagaag
    39601 ttaatcagta aacaaaaatt agaaaatact agtgggctat ttattcatcc ggcgattgac
    39661 gctacttttt cgtttttata ttggtacagc gatccttaca accatgaccg tttcatggac
    39721 ccgggaacgg attctgtagg cattgctttt tctaaagaaa atatgttagt cttattgcaa
    39781 gaagaaaacg gagaaagtag tgatagcagt gatcgttaac gaagagctgt ttcaactgga
    39841 ggatcaatgc ctccaagtcg ctgaaatgat aaaaaagagt aaaacgatgg cttcttatct
    39901 tgaacataaa cagtcaatgg aagaatctgc aaaagtttgc cagttgaaag aagcgttctt
    39961 gtctaagaaa gaagcatttg aaaatgtagc ggcatatggt gagtatgcac ctgattatcg
    40021 tgaaaaaaaa cgagctgttc gaaaagcaaa aagacagctt gatatggatg agcatgttgc
    40081 agctttccga tatagtgaaa cacagctgca aagtctgttg gatcaaattg tccaagtact
    40141 tgccgaagaa gtttctaatg aaatcaaagt agatgcaggt agcccgtttt ttgaaacgaa
    40201 aaaacacttt ggatgcggag gtaattgtca tgcaattaga caatagtgaa tttgaaataa
    40261 caaaacgacg cgggctgatc gtatgggtct acagcttaaa acaattaaag aatctgaaaa
    40321 agttcggctt gttgcattat gtctcaagaa gaatgaaata tgtcgtgctt tacttaaatg
    40381 aagagacatt ggaagaaaca gaagaacgaa tccgtaaatt acattttgtt cgaaacgtag
    40441 aacgttctta ccgaccggat atcgaaatga attttgccga aaaaatagga aacaaaacag
    40501 tattaaaaga cgaaggtttc gaaattgaag agctaaatac agaaatccga ttagctgaac
    40561 atctttaaaa atctcttatg agcagttttt aaataaaaaa taacttggat aacgatcgat
    40621 ttttgatcat tgtccaagta tttttttggt atactagtac cagtaccatt tctaaggagt
    40681 gagtagatgc ggataatatc aggaaattat ggcgggcgcc gtttaaaaag tttagcaggg
    40741 gcgaacacca gaccaactac agataaagta aaagaatcca tattcaatat gattggacca
    40801 tattttgatg gagagacagt attggatctg tttgcaggaa gcggtggttt agctatcgaa
    40861 gcagtatcta gaggctgcag tcatgcggta tgcgtagaca aaaattacca agcgctaaaa
    40921 atcatcaaag aaaacatcga aattacgaaa gaaccagaga aatttaccac aataaaactt
    40981 gatgcggaca aagcaatcga acggctagca gcggagaaac aacaattcga ctacttgttt
    41041 cttgatccgc catatgcaaa acaaaaaata atcgatcaaa tccataaaat ggaagaactg
    41101 ggactttttt ctaaaaccgc agttatcgta tgcgaaacgg ataaaacagt cgaattgcca
    41161 gaaactgtgg ctggattcca tcaaataaga aaacagattt atggcataac ggccgtaact
    41221 atctatagaa aagagggaaa tcaatgaaac gtgcattatt cccaggaagt tttgatcctt
    41281 ttacaaaagg tcacttagat acggtggaac gtgcggctaa attattcgat gaggtagtaa
    41341 ttggtgtttt tatcaatact tcaaagaaaa gtttgtttcc cccagaagaa agaatgacat
    41401 tgatcacaaa agctgtttct catcttccta atgtaaaagt catgcatcaa gaaaatcaat
    41461 tgacggtaga aacagcaaaa gagattggtg cagatgcgtt gatcagaggc atacgaagta
    41521 tcaaagactt tgaatatgaa cgagagattg ctcagatgaa tcatcatcta tatgatgaat
    41581 tggaaactgt cttcttatta gcaaaaccag aatatagtca cgtgagttcc agtattttaa
    41641 aagaagtact gcactttggc ggagatgtga gcagctactt accgccagtg atcaacgaag
    41701 ctttagctgg aaagcgtgaa tcaaatgaaa gctaaacaat ggttgaaaac agtactgatt
    41761 atcatatttg gaatgttgat cattggattt gtagtagttc ccttgccgta ttatgtagaa
    41821 tcaccgggag ccacgatcga tctgaaagaa ctaattacgg tcaatgataa ggaagataaa
    41881 ggttctgggt ctttctctct tacctctgtt ggtatcaggc aagctacagg tttcttagct
    41941 ttaaaagcta aaatgacccc ttttgaagaa ttgatcagtg aagaagaatt gactggtggg
    42001 gcgacaaatg aagaatataa tcaaatgcaa cagtattata tggattcttc ccaaaatgca
    42061 gcaatcgaac aagcgttaaa acttgcagac aagccttata aaatggaatt caaaggcgta
    42121 tatgtcatga gtgtagagaa aacatcgaat ttctttggca agctttcagt tggagataca
    42181 gtaacaaaag tagacgggaa aagctttcaa agtacagaag catttatgga ttatgtaaaa
    42241 agccaaaaag ttggacaaac aatcgaaatc agttatttgc ataatggtga agaaaaaaca
    42301 gcttctggtg atctgatcga gttaccaaca gataaaaagg caggaatagg tatcacgctt
    42361 actgaccata ccgagatcga atcagataca gatgttcgta ttaattctgg ctctatcggt
    42421 gggccatcag cgggattgat gttcacttta gaaatctatg aacaagtcac tggtaaaaac
    42481 ttgcgacatg gtaagcaaat tgcaggaact ggaacgatca acagtgaagg agaagttggc
    42541 cgaatcggag gaatcgataa aaaagtggca agtgcggata aagctggcgt agagattttc
    42601 tttgcaccag atgatacgat ttcagacgat gttaagaaag aatatcctga aataaaaacc
    42661 aactacgaag aagcaaaagc tgccgctaaa aaattgaatt cctcaatgaa aatcgtacca
    42721 gtaaaaacag tacaagaagc gcttgactat ctagagaaaa tgaaatgaaa ctaaataatt
    42781 aaagaggggt tgtgacacga gttctgtcac aacccctctt tttcgtcaaa aatcttcacg
    42841 ataaaaataa aaacaaaaga aaaaattgac gtaattagat gttgaaggga cgattttgcc
    42901 agagctcact gtttcttcaa ttttaaatga aaggagtaat gaatgatttt tactggtcat
    42961 tctttgaaaa aagaacaatg atagaaaaaa tcagcaagaa aatactagct tcacccaaat
    43021 tgatcatttg gatgtctatg atagttctta tcttttgtat cgcaagtggt tttttattat
    43081 caaatttgct attttataga gaaacagaag agactgctgc aaaagaaata ctttcttcgt
    43141 ttagttccag tggagaagat ccatcagctg aaggaaacga tgtagaagag gatgctcgtc
    43201 caaaggtcat gtacaccgat atcaaaggaa gtgtgaagga gcctggtatc tattcctttt
    43261 cttcagaaga acgggtctat gatgtactaa agcgtgcagg tggattatta gaagaagcgg
    43321 atagcgatcg aatcaacttt tctgcgaaga tagaagatca gcaagtccta tatatccctg
    43381 ctgtaggtga agagctgcca gaacatcttg atcagttggc atcctcggaa ggaaaacagc
    43441 ctacagcaga ttccgagcct cccaaaataa atatcaatac agccactcca tcagaacttc
    43501 agcagattcc tgggatcgga tcagtcaaag cgcaagaaat cattcgattt agagaagaaa
    43561 acggatcctt ccagaaagtg gaagacctgc aggagatttc tggtatcggt gaaaagacag
    43621 ttgaaaaact aaaaaacttc gttacaatta aatagaatga ttcaaaatcg actggcacga
    43681 gcaataaatg gacgctaatg gatactaaga ataatccaat tctaagagct tggcaggacg
    43741 aggaggtagt atttttggat aggaaatagc ttgattgttc gcaaagaaac attaaaaaaa
    43801 ccaatgctga atgtctatct ccaaaataaa atatctggga ttcatattat gaatacggct
    43861 gtcagcggca ataacagtca agctctacga gaacgctttg ccaaagatgt acttagctat
    43921 acagcagata aagtatttat tctcattggg acgaatgatc tagcagagca taaacaactt
    43981 tctaaagaaa cgtaccaaaa aatctgcagt ggatgatagt tcagctgaaa aaagactata
    44041 gttctgatca tgctttcttt atcacgccac cagcagtcga tgagacaaaa caaaaatatc
    44101 ggaataacaa gttgatcaaa gaatatatct ctgttctcac agaagtagca aaacgccatg
    44161 attgtgaagt atcgattttt atacggaatt tcttgaacaa atggattcga agaattttta
    44221 taatgggcta atggatgacg gacttcattt cggcagtgaa gggtaggatc ttttgtcaga
    44281 caggatgatt cgttatattt aaagaatatt ttgtttaagc aatgagtact ttttaatcaa
    44341 cttgaaaaaa cggaggaata taaaacatgt tagaaaaatc ttcaaaagca tgcgatacga
    44401 ataaagaaaa agttactgat aagtcacata atgaacgcat tccttgggac cagtacttta
    44461 tggctcaggc agtactgtta tctttacgta gtacgtgtac acgtttagaa gtaggagcga
    44521 cactcgttcg agaaaaaagg atcattgctg gcggctataa tggagcagtc agtggagatg
    44581 tccactgcat agatgaaggg tgttacgtcg tggatggaca ctgcttgcga acgatccatg
    44641 cagagatgaa tgcactccta cagtgtgcaa aacttggtat cccgacggaa ggttcagaaa
    44701 tctatgtcac acattttcca tgtttggcct gtacaaaggc tttgttgcaa gctggtgtga
    44761 aagggatcaa ttatctccat gactatcgaa atgatcctta tgcccaagcg ttgatcgaac
    44821 aattaggtgt atctgtacac caagttgctt tagactcaca gcatttttca aaacttcaaa
    44881 atgaattaac aggaaaacat gtctagcgtt ttagaaaggc atgttcgaaa tcggttgatt
    44941 tttccggcaa ttacgtcgat gattatctgt ttgttgtatt acactgacaa ttggggttat
    45001 cgtttgcttc ttgccggaat ttttttatgg ttttcgctaa aagaaggacg ttttatcgcg
    45061 tacttgtgta ttttgtgtag cataacggta tccatttctt ataggctaca agagtattca
    45121 tttttagagg aaaaaacaac atctgcgcaa attactgtat ttacagatac gattcgtgca
    45181 aatggtaatt ttttgacaat ggaaggaagg ttatcagaga ataaacaaaa gctccagctt
    45241 tcttatcaag taaacacaga agcagaattg aaaacatggc ttaacttccg aggaaaggga
    45301 atagaattga ctgctgaagg cgagttcatc tctccagcaa aaaagcggaa tctttataat
    45361 tttgaccaag aagcttactt caggaaccat agaatttctg gacggttcca aattcaaaag
    45421 attgaacaga tcaccatgca aaatcactgg cgaaactggc ttcagagaaa aagaggatct
    45481 tttatctcct ttgtccaatc tgaatttccg caaaaaacaa gtgtatatat taattcttta
    45541 ttatggggct ataaagatgc aggattcaga gaaccagaag aaatttttag ggagagtggt
    45601 ttgctgcatc tgttcagtct atcgggtcta cacatccaat tttatttagg gtggctgtat
    45661 tatttatttc gacgtatcgg atggccacta cacgtcagta ttattccgtt gagtctacta
    45721 actatctgtt atgtttctat tgcaggaagt tctatttctg ttttgcgtgc tagtttgctg
    45781 tttttattgc gattgctagt caaattgctg aataagcatt attccaccat ggacttattt
    45841 gcagttgtct tttggcttct tttactattt gatcctcgat ttctttttca tgcgggtgga
    45901 cagcttagtt ttttcatgag ttttttgttt atcctgattt cttttgatgg agattggttg
    45961 aaaaaaacgg cctctcacgt cctgtttact atgatcacga tgccgttgat tgtatggcat
    46021 ttttatgagt ggcctatttt aggtagtctt tttacattac tgattgcacc ggtattcaag
    46081 tttctttttc ttcctggtgt gttatttctc acactcttca atcgctggct gccggaaact
    46141 ttacttttgc tgatagaaga gggattaatc ttatttgagc gaataatcga tttttcttca
    46201 gggtcgtctc tcattatagg acgtttgccc cttgtgctat ctgggatgta tattttcgga
    46261 ttactgattt gtatggatag gttgaaagaa caacctgtga aacacttgtt ttatgttggt
    46321 cttttttctt ttattttttt gactttgcca tttttacgct tttcttcaac gatcgctttt
    46381 atcgatgtcg gtcaaggaga cagtatcttt ttgcaaagtc cttttcgaaa agaaacgatt
    46441 ttgatcgata ctggaggtca gttacacttt gaaagagaaa agtgggcgca aggtatgata
    46501 cgtcctcctg ctgaaagaaa tatcgttcca tatctaaaag gtcaggggat aagtgtaatc
    46561 gataaactca tcttgacaca cgacgatacg gatcatgtgg gagaattacc cacgttatct
    46621 cagcatttta ctatcaaaaa aatttatatc ggttggggcg ctggacgaaa tgagccatta
    46681 caaaggcatt tatctgaagc tcaaaaaaat ggtactgaga tcattgaaat caaacatggg
    46741 gacaggatca gcggttatta tgatctttat gtattgactc cttttgaaaa gggagaggga
    46801 agaaatgaag attcgattgg tctatggatg gaatataaca ataggcgctt tttattttta
    46861 ggagatttga atcaagcaat ggaaaaacaa ttgcttttga tttatcctaa tctgaaagcc
    46921 gatgtagtca aattaggtca tcacggtagc cgtacgtcaa gtaacacaga ttttctttcc
    46981 catattgaag caaaacatgg gattatatcc tgcggcgtaa ataatcgtta tggtcacccg
    47041 cacagcgaag tattggagac actagaagaa aatcagataa aaacatggcg aacggatcaa
    47101 caaggaatga tcacctatcg ctggcatcca gtttttcatc ctcatgggct agtggaaaca
    47161 atgatagact agtaattctc atctgctcat ggtaggataa tttttgaaag tggtgagtaa
    47221 tttgagtata caagcagagt tacaaaaaat acgtaaagaa aaacttgcat cttgttactt
    47281 gattttagga acagaaaagt ttctccaaga tcaagttcgg acagaaattg tcaaaaaaat
    47341 ccaattagag ggcgaagatg acttaaattt tttaagtttt gacatggaaa atgcaagttt
    47401 agatgaggta gtagctgaag cagagacgtt gccgtttttc ggagatcaac ggttagtatt
    47461 cgtagagaat ccttattttt taacaggtga aaaagtaaac aatggaatcg aacaaaatac
    47521 tgatttattg acggcttatt tgaaagaacc attagaatcg actgtattag tattctttgc
    47581 gccatatgaa aagttggatg aacggaaaaa agtaacgaaa caattgaaaa aaacagcaat
    47641 tatcatcaat gtccaacagt tgaatgaaaa agaagtacgt caatatctga tgaatacctt
    47701 agagaattcc tcaatcaaaa tggaacgaag tgcaattgat ttattcttga ggttgaccga
    47761 cttagattta tccaaaatga tgagagagtt acagaaattg gtcctttatg gacaaaatca
    47821 acagcaaatc acggtaagag aagtagaaga attagttcct aaaacgttag aacataatat
    47881 ttttgacatg acccaatata ttttaaaagg gaagacggaa caagctttgc gtttatacga
    47941 agatttagtt atgcaagggg aagaaacaat caaaattaat gcgattttgc tttctcaatt
    48001 acgtttattt ttacagacaa aatttcttgt aaagattggt tatcagcagg caaatattgc
    48061 tgaaacattg aaaatccatc catatcgtgt aaaacttgcc atgcaggaag taaaaaaatt
    48121 cgatgaacat ttgctggtac agttgtttga tcaattagtt gagatggatt atcaaatcaa
    48181 aacgggacaa atcgaaaaag aattaagttt tcaactattc gttttgcgca cagggcagtt
    48241 gttaagaaat cgataagaag aaagacgagg gcaaagcatg cgggaaggtc gtttgttttt
    48301 gtcctttttt tgacaaataa aaaaatcggc cgaagccgat tcttttattt agcgattttt
    48361 ttgcttaaac gagatttgtc gcggttagct ttgtttttgt ggattagacc ttttgattca
    48421 gccatatcaa tagctttaac agcttcttta tataacgcat ctacgttgtc agcacctgaa
    48481 gctacagcat cttcaaattt tttgattgca gtacgcatag cgcttgtttg agatgaattt
    48541 ttcacattag cgttttcgct tgtacgaaca cgtttgattg cagattcaat gtttggcatt
    48601 agtttcacct ccgataactt aagttcttac gcgtgttacc gcataattca acattcatta
    48661 ttatacctaa aggcagcgtg ctttgcaata tgaacatgac aagttttttt ccttaacagt
    48721 cttttattag gatttttttt cagtaaaaac ggatagtaca agctgaaaaa aatcaaaaag
    48781 acgctattgt gggaggcaat agcgtcaaac caaactagat ttttatttat ctatttaaac
    48841 aaagtttgaa tgggaatgtt tggtactaag ttattatata acaaagagaa ccgtttgcaa
    48901 agtttttttt gaaaagggat acgattccct gttgttcttt gtttttctat taagaaactg
    48961 cagttatttt ataaccagta tcctttgatc agtaaaggct attctggtgt accgattatt
    49021 ttaatgtatt catcgatatt ttttatcaac atttttttta tgaccaactg gttccgacgg
    49081 ttccatttgc cacttacttc cagcacatat ttatttaggg gcaaacgtaa gatatatttt
    49141 gccaaatagc gatctttaag caggccattg atcacacggt cctcaagctg caagttgatc
    49201 ccagtcagca tcattggctg ttcttgtaaa acgtacgtat caattaaaag accttcaaaa
    49261 gtttccacaa gcgtcttcct ttcttttcaa agtaacctga aaacaggcac taattcttcc
    49321 attttgatat tcgttgaagt ggatgcgatc attctttctt ttgtccactg taatctagta
    49381 taacaaaaaa gagcagaaga gccaaaaaga aaaaagagca tcgaattcta taggaagtct
    49441 cattggcacg gcctaaatta ttacaaaaca ttgattaaaa aaagcccctg catcgcagga
    49501 gcatccccta tatcgtttta ataggtcaat caaaaagcag tatcaaacac attttgtatc
    49561 aaatcaggaa ataatagata agctaagccc agaatcaaaa gtaccgctag aagaaaccag
    49621 ccgattgttc ctatgaaata ctttaatttt tcgaacattg tatcacctcg ttgtcagtat
    49681 atcacggatc agtaaattgg gggtgttaag aatctcctaa aatagacaaa atttgttcta
    49741 ttttcctaaa gcaaatactt gtaagataca gtaagtgata ccagaagcaa tgaccggtcc
    49801 ggcggcgatt cctcttaaga agacaacacc taaaatcgtg ccgaaaacaa gtgcaacagt
    49861 gatttgtgga tcagaagcaa tcaatcctac acccttggaa gataagacag ccactagccc
    49921 accgcaaaaa acagctaccc agccaatagg ggatttaaag gcttctaata gatcacgtag
    49981 accgatttct cctgttgcga tagggattag aatagccgct gtgatgatcg tcaccccaat
    50041 attgattcct ttgccttgaa gccaagggaa aaaggaacgg gaggctggta tgagcttcat
    50101 aatcaaaaca aaagctgccg ctatcatcag actttgattt ttagctaaaa ttgctacaag
    50161 taaaataatt cctaaaaaaa gccaactttc catgaaatat attcctcact ttcttcgaaa
    50221 caatcataac atgtttttag aaagttacaa aagtaggaat tcatccttca agacctattc
    50281 ataagctgtt tttttcataa aataaacaaa atcataaaaa aacactttgc ttatttgaca
    50341 gaagacagcc gcgctttgta tagtggacaa gacttacatt tttttgaaga atcagaaaga
    50401 tcaggtaaag gtcttagtaa acgcaagcca ttcagaataa ccaatattgt gcttccttcg
    50461 tgtcctacaa ctccaagagg tagatttaaa aattgcagga agttagaagc aatcaacaag
    50521 agaataacaa gaatcgaaaa aataatattt tgtttgatga tccgccgcat ttttaaagat
    50581 agccgatgac tgtagacaaa tttatttaag tcgcttttca ttaagacgat gtctgcgata
    50641 tccatagcgg catctgtccc ttgccccata gcgacaccta aagaagcggc tgctaaagca
    50701 ggcgcatcat tgactccatc accaaccatc gcatttactt ggtaaagttc attctctttt
    50761 ttgactagtt gtgttttttc ttccggagta cagcttgcat agtaatgttc aatccctact
    50821 tgttcagcaa tggcctttgc tgtgccagaa tggtcgccgg ttatcatact tgtatggatg
    50881 ttttgggatt taaagtagga aattacttgt tgggtatttg cttttggaat atctagcaga
    50941 cctaaaacag caattaactg ctgatcttta gaaagataaa tcacggtttt gccttgattt
    51001 tccaaacgtt cgatcatttc cgcaatctct ttgctgatta tcatcgtctc aggatcatat
    51061 gcgtgcttgc caactttcca ttgcgcacct aaataggtag tttgtaatcc aaaaccagta
    51121 atttcttcta ttggtaagta ttggatttct tcaggtattg tgcaatcgaa atattgcaaa
    51181 atcgcatgag caagtggatg cgtcgttttt tgttccatag caacaagaag ttgttgtgcc
    51241 aaatcctttt catcaaagaa aaagctgtca gttacaactg gttttccttt agtgagcgtt
    51301 cctgttttat caaaggcaat cgcttttaat tcagaaagct tttctaaatg gataccgcct
    51361 ttgattaaaa tcccattttt tgcagaattt gaaagagcag ctagagaagc tggtgtagca
    51421 gatgcgacaa gcgcacaagg agaagccaca actaataata ccattcctcg ataaaagctt
    51481 tcatcccaac tccaacctag gaaaaagtgg gggagaagga tcattagagg aacagcaagc
    51541 aaaatcaatt ttacataatt attttcgatt cgttcgataa atgaagctgt ttgagttggc
    51601 gtattctgcg cttcttctac taattggata atcttagcaa agacagtttc ggaactcgtt
    51661 tttgttactc gaatcgtgat gggatttcct tgattcagcg ttcctgcata catctcatcg
    51721 cctaaatatt tttctacagg cacagattca ccgcttaatg cagactcatc gatcgttgcc
    51781 tgtgtgcttt ctaaaaagcc atctatcgga acagctgctc ctttagcgac aaataccaag
    51841 ttatccacct ttagttgaga aacatctact tcttgcattt gtccattttc ttttaataat
    51901 tgggcttttt gtggttgaag attcatcaaa gcagtaattt ctttttgact tttattagtt
    51961 gtgtattcct ctaatgcacc gcttaaacaa aagatgaatg tcaacattgc tccttcaaag
    52021 taattaccga tcaaacaggc accaatagca gctaaagcca tcaataaatc cacattcagt
    52081 gtccgatcat tccacaattc tttcaagcct tccgatgttt gtttatatcc accgatgata
    52141 atggcactga tataaaaaag tggggtgatg gtaagagaga atcggtcgaa gataaaaccg
    52201 aagatcataa ataaaaggca gccgattgtt gctgcaaaag cttttttctg attagtcatc
    52261 gttattagac ctccttgtca aaacaaggat agcataataa aaatgtaatt gataataata
    52321 acagctaaat gagaacgata atggttatca tttggtgatt aagcttgatg ttgtttttat
    52381 tatttataat gattattatt aaagaaaata aaaaaccaga acatttctca aataagagaa
    52441 gtgttctggt tttttctaca ttgcaataaa ccaataacca atcaaaatga taccaaggat
    52501 aatacgatac cagccaaaag cggtaaagtc gtttttcttc aaatagctta gtaagaattt
    52561 gatagctaag acggaaacca caaaagatac gaatgttcct acaagaagaa caacagtttc
    52621 ttccattttg aatgaattcc cttcagctaa atatttgacg atcttcaagc cgcttgcgcc
    52681 aaacatgaca ggaatcccta agaaaaatga aaactctgta gcgacaaaac gcgaagcacc
    52741 aatcaagata gatcctagga tcgttgcgcc agaacgggat gttccgggaa caagtgccaa
    52801 gacttggaat aacccaacaa gaagagctgc ttggtacgta aagtcattca gagaagtcca
    52861 acgaggtgtt ttgtttttgt tgcgtttttc gacaataata aaggcaatac cgtaaacgat
    52921 caaaacaatg gcaactggta agaacttgtg gaaatttgct tctaaccaat catccaaagg
    52981 caaaccaatg atgatggctg gtatgcaagc aacgatcact ttaaaccaaa gtgtccacgt
    53041 atcttttttt tctaccgttg atttacttgg agaaaatggg tttaacttgt gaaaataaag
    53101 aacgacgaca gccaagatcg cccctaattg gataacgaca ttgaacatcg acataaattg
    53161 tgaactggca tccagtttga tgaattcatc tgctaggatc aaatgaccag tactactgat
    53221 gggcagccat tccgttattc cttcaataat accgagaatc actgctttga taatatttgc
    53281 aaaaaacata aattccttcc ttcccaatta agatacatct tatagtatac cgttattttt
    53341 tgagaaaccc aatctgtttt ttaatattaa gaaaaacttg agaaataaag cagataagta
    53401 cacgaagaga aaaaagaaga caaagccctc gttcagactt tgccttttat gattctctgg
    53461 taaaaattaa aatatcccca ttatagaaac gagtcgcgac tatgtgtttt cccgttatcg
    53521 atataagacg tatcattgat gccgtggata gaaaatcgac cgtttgcata agtgaccttt
    53581 gttacgctgc catttaaaag cccagcacga acaggctgtt taggatcgat cagattaagc
    53641 aaaaaggcga tcgtcaaacc atgagatacg actaaaacat ttccaccacc atttttttcg
    53701 cagtgatggg cgatatcttc aaatcctgtc catacacggt ctcgcaatgt ttcataagtt
    53761 tccgcccacc cagcggtatc taagttgtat acagcattag caatttgttc aaaagaaact
    53821 tctgtggtaa acatttcatc atatgtttta aaattcaata cacgagggat cacaccccac
    53881 atttccatgt catatccacc ttcaagagac ccgaaacacc attctctaat ccttttttca
    53941 ataaaataag gaatttcttt tcccttagaa tgttctccta gaataatacg ggcagtttcg
    54001 attgcacgac cactgtcact agaataagca aatgaaaagt cgacattttt taacccgcga
    54061 cctaaataat gaatgccttc ttgtccttgc tttgtgaggg gtgtatcaca ccagccttgt
    54121 actcgttcaa ttgtattgaa catcgttttt ccgtgtcgta cgatataaag tgtagtttct
    54181 gtcattttat tctcctccta agtcgtttta acgattgaaa aaaggatttg tccttttttc
    54241 ctgttcaatc gtcgttgcat cgccatgtcc tggatagact gcaaattcat caggtaatac
    54301 aaacagctgg gtccgaatac tatgaagtaa ttgttctaaa ttccctgtat acagatcggt
    54361 tctaccgata cttcctctaa acagggcgtc gccagaaacg acaaaatcat caaaaataaa
    54421 gctgacgctt cctatagaat gccctggggt agggacgacg cgaaaagaca tgtttcctaa
    54481 agtataagtt ttcatttcaa attcatattc tgcaggcgag acaataacat cagcaatatc
    54541 atcatgtcta ccgagtccag aaagattcaa aatcggatca cctagccatt tttgctctag
    54601 tgggctgaca taaactggga tttgataata cgcgcgtaaa ggttccactg caccgatatg
    54661 atcgtagtga gtatgggtaa gcaaaatcgc taccggctgt tggtttgttt ttttgatttc
    54721 ttgtttgatt cgttccgcat cttctccagg gtcaatgatc aaaagtgctt cttcgttgta
    54781 gaccaaataa cagttttctt caatcattcc tgtttttaat ctttcgatat gcatgtgcat
    54841 tcctccttga tagccaaaac ttctttcatc cattagtata acgcattcat tgctaggaaa
    54901 caaaaacagg tacttatttc tctttactta tctgaattat cagactatat gaaaagtatt
    54961 tggatattct aaagagaagg aaagtaagtt ttcatagcat tttaatatgt ccttgttaca
    55021 ttagattcat ccaaaaatcc aaattctata tcctatatcc ctgcctaatc agcagggtgt
    55081 ttttttatgt ttggaaagag agctcttttt gctaaactag gttttttcag gtaatcttag
    55141 agagaaagag gtggtggaaa tgatcaaaca gtcaaaaata tctgtgcggc acttagtaga
    55201 atttattttg cgacgtggaa gtatcgacaa tcgtaaaaaa agtaaccata ctgcattaga
    55261 aggagcgaga atccaccgca agctccaaaa agatgcggga gaaaactatg aaaaagaagt
    55321 ctttttaaaa acaacagttc aattagatga atattacctg atcgttgaag gccgtgcaga
    55381 cggtattttc aaaagagacg gtatttatta tatagatgaa atcaaaacct ccgaaccacg
    55441 atttgaagat ttagaaccag aacaagtgga acttttcttt catcaagcaa gggtgtatgc
    55501 ctatatctat agtcaagaac atgatttaca agaaataaat ctgcaattga cttacttcca
    55561 gacggcagaa gaagtgatca cgagaaaggt tgaacatcag acaagagagc agttagaaac
    55621 tttttttaaa aagctgactg aagattataa agaatggctt gtttttcaag aaaactggcg
    55681 cactgtacgc aatgcctctt tgatggcttt gaaattcccc catgatgagt ataggaaagg
    55741 tcagcgtgaa ctagcggtag ctgcttataa aacgatccgt acgaaacaga aattgtttgt
    55801 agaagcacct accggaacag gaaaaacaat ctctacgtta ttccccgctt tgaaagctgt
    55861 aggggaagaa gagggagaaa agatctttta tctaacggct aaaacaataa ctagacaagt
    55921 agctgaagat gctatgacag cattaaaaga tacaggagct gaagtaaaaa gtgtcacttt
    55981 gacggcgaaa gacaagatct gctttttaga tgagacgacc tgtaatccag atcagtgccc
    56041 atatgccaat ggttattaca atcggattaa tgaaggttta tgggatcttt tgaatcatga
    56101 gaatcagatc actcgtgaag tcatcgaaac ttatgcaaga aagcatacac tttgtccttt
    56161 cgaactatca ttagatgtca gtatctggtg tgacgtaatc attggggatt acaattattt
    56221 atttgatcca actgtttatt taagacgttt ttttgaagat gaaaaaaacg aggactatct
    56281 gtttttgatt gacgaagcac ataatttggt gaatcgttcc cgagagatgt attcggcaga
    56341 actatcttat gaaaaaacga aacgaagtaa ggaagctata ccaaaggaat tcaaaaaact
    56401 tcatcgccgt ttcaacaaac tattaaaaga atttgattcg atccgagaaa tagcaaaaga
    56461 agatcattgg gattatcatc atcaaaaagc accggctgaa tctttagtaa aggcaggtta
    56521 tcaattaagc gaaaaaatta aagaatggct ggcagaattt cctgagcacc catcacaaga
    56581 acaattgctt ccttattatt ttgatttgct tcattttttg aaggtcagtg aattttatga
    56641 tgatcactac gaaacaacag tcgaaaaaac gtatcgcgat ctgatagtaa aagagttttg
    56701 tattgatcca agcttgtttc ttgagcagtc gttggataaa ggaaaaagca gtattctttt
    56761 ttctgctagt ttttcaccac tctcttatta tcaggaaaca ctggggggac aaaaaagtct
    56821 ggcttacaaa ctgccgagtc ctttcccaga aggaaaccag caagttttca tcgcaaatta
    56881 cctagagacg acttatcgaa aaagggaaca cagtctggca aaattagtag agacgattca
    56941 tgcttttgct gatggaaagg tcgggaatta tttcgttttc tttccttctt atcaatattt
    57001 agatcttgcg gtggaagcat tcagacagca atatcccgac agcaatgtgc taatccaaga
    57061 aacggacatg aatgaggaag aacgcgaacg atttttaaca aaatttcaaa caaatcccgc
    57121 tgaaacattg attggatttt gcgtacttgg cgggattttt tcagaaggaa tcgatttgag
    57181 aggggaccga ttgatcggca gtatgatcgt aggtgtaggt ttgccgcaga tgaatcatga
    57241 gcaggaactg atcaaatcat attatgatga gaaggaacac cttggatttg cttacgctta
    57301 tcagcttcct ggaatgaaca aagtcttgca agctggtggt cgagtgattc gtggcatggc
    57361 ggatcaagga atcgttgtat tggcagatca acgattttct agtttttctt atcgacgttt
    57421 attcccacaa cattggcaaa cagcaagaga agtacgaagt gttggggaat tggaagaagg
    57481 aatacagcgt ttttggttat caaaagaagc agtaaatcaa aaaaacgagg aatgacagac
    57541 gtcagcctcg ttttttgatt aacgatagtt agtgaattgc aactcgattg gtaagtcaag
    57601 tttacgtaat aaagcaatca cagcttgtag atcatcacgg ttcttacctg tcactctaat
    57661 ctgatcttct tggatttgtg cttttacttt cagtttttca tttttgatag ctgtagtgat
    57721 tttcttcgcg ttttcacgat cgattccgtt gaccaaatcg gcagtttgtc gagctttgcc
    57781 acctagagcg tgttctgatt cggaaaaatg gatatttttg attggcacat ttcttttgat
    57841 cagcttacca aataatacat ctttgatctg ttcgattttg aaatcatcat cgctcaatac
    57901 aactagattg ctgttctcta atttgatttc tactgtagaa tccttgaagt caaagcgatt
    57961 ggacaattct tttgttgttt gctgaatcgc attttttact tcttctgtat tgacttctga
    58021 tacaatgtcg aagctagcat cttttgccat aggaaccctc ctgatataca tataaatatt
    58081 taccatacat ctatcttatc ataggattag ggcagattct tacctgtttg ctaaaattct
    58141 tctcatttga gttcgaagta ttttatttta atagataaac tgttattctt acatttggaa
    58201 taccaaagta ggggagaggt gcttgtgagt aaaattatga ctgcgtatca taaaaatgaa
    58261 agattaaaga aaatagtggt tattttgata accggattga ccgctgcggt cgctttaaat
    58321 tattttttga ttccagccaa tgtcttttct gcagggatga atgggatcgc tcaaatcatt
    58381 tcctcgcttt tatatgaatg gtttcatatc aagatagaca caggaacatt tatttttctt
    58441 ttgaatatcc ctgtgtttgt attagggttt ctcaaagtag gaaaacgagc aacgatcctt
    58501 agttttgtga atgttgtatg tgtatcggtg atgacgatgc tgcttccagt cggacagatc
    58561 actgataata ttttgatgaa tgccctagta ggcggtgttt tattaggtct tggcgtagga
    58621 ttatctttaa aaatgggctt tacaactggt gggatggata tcatctctct tgtactttca
    58681 caaacaactg gaaaaacagt cggaaactac atgctgattc taaatggtat cattgtagtc
    58741 atcgcaggct tcttctttaa ctgggaaagt gccttgtata cgattatttc catttatgca
    58801 atgactcaag taattgatgc catccacacg agtcatcaaa aagtaacagc aatgatcgtt
    58861 accgtcaagc cagaagaggt aacagaagaa ctagcaaaac ggatggtacg tggaatgaca
    58921 ttactgccgt ctgtcggtgg ctattcaaaa aaacaaggga acatgatcat gatggtcatc
    58981 acccgctacg aaatgtatga tcttgatcag atcgttcatg aaatcgacga cgatgcattt
    59041 atcaatattt taccaactca ttcagtattt ggccggtttg caaatgaaaa cgaacaaaaa
    59101 atgtatcgaa caactggcgt attgccagaa ataaaaaatc gttcacaaaa aaaatgagga
    59161 atcaggttta ttttagaata aatttaatct attacatcta ataagaacat ttttataaat
    59221 ttaaacgaga atcttacttc taaattctca tttacaagca gctaaaaaaa gagtatgata
    59281 aataaaaatc gtgttaagga gtgtcgtacg atgagcgaag caaaccaaga acggtctgga
    59341 caagaaatag aactaatcaa agaacgtatt cttgctgcct tggaaatggt gattgatcct
    59401 gaattaggaa tcgacatcgt caatttaggc ttgatttatg atatagaatt caatcctgaa
    59461 aatggagaaa cagtcattaa gatgactcta acaactatgg gctgtccatt agcagatatt
    59521 ttaacagaat ctatccatga cgcattaaaa gaagtgccag aagtaacgaa agcagaagtc
    59581 aaactagtct ggtatccagc atggacgaca gataagatga gccgttatgc acggattgcg
    59641 ttggggatac gatagaaatc ttggctgact agctatgaga agcaaatagg aaaaacaaaa
    59701 aagcctttta tcctttctag aagaaaagag ataaaaggtt tctttgttta taaaattttg
    59761 tctgctatat tataagttaa ttaaataaat gtgttctatt gataaaggac attcaaatcc
    59821 ctttcctttt ggaatccttc ttttttacat cagtttgatt ttcgtatagt attctttctc
    59881 atcttcacga taaatcgcag ccccattttt ttgttgtgct ttaagactgc caagattttt
    59941 tttatttaca gacaaaagtg cctcatcaat gccgattttt tttgcctctt gtagtactaa
    60001 tgctaatcct tgtgtggcat atcctttttt acgataatta ggggagatgc cgtaaccaat
    60061 atggccagcg ccgtttctta atgtttcgtt cagtttatgg cgcaaattaa aaattcctac
    60121 atactcatgt tcatcacttt ctaaaataaa gacggttgcg ggaacgtaac tctcaggaag
    60181 atcgattcct aaagacgttt tttcgcattt ttttacataa tctaaaaatt cagcaaatgt
    60241 atagccataa gcattgttga taaatccact ctcatttggt ccaaagctag tttgtagctg
    60301 ataagctttc tttaaatctg cttcccatag tttgatcagt ttcatcgtat tcacctcttt
    60361 aagtaacctt attcatttgt tttctactat agcactattg ttgtaaatag ccatgcctat
    60421 ttttatttat tgtgtcatat cgtattaaga atcatcgaac aatacaaata tgaattacaa
    60481 taaaaaaatt atttgaagtt cgttcattcg ttacttatga tctctagaag aaagaaaacg
    60541 tgaattactt cagcgtctag aattccagaa ggatagtaat caacagaaga aataatcaat
    60601 atccaaatga aagaagatag ctgaatcgag aaaaagagga aaaaagaaat gaagaaaaaa
    60661 ataaaatagt tactaatccc agagtcgttc gtttaacatt gaaatcgatc atatggataa
    60721 aacagatttt ttcaccaacc ctcaatagat gatggagttg ctcggatgaa ttatttgtgt
    60781 agatggcata agggagggcg tacagaaatg aaaaagaaca aaaatatgtt cttgacaaaa
    60841 aacacatgta atcctaaact aaaagatgtg atgcggctat ggcggaattg gcagacgcgc
    60901 tagcttcagg cgctagtgag ggtgacctcg tggaagttcg agtcttctta gccgcataat
    60961 ttgttttttt ataaagagaa ggtacacctg gagcgatgag aaagaaaata acatattatc
    61021 taaaagaata taggaaaaat tttaaacaac ggaaccagat tcaaataaat caaaaaaaat
    61081 ttgtgtgaag gtgggacagt ttaaaaccta atagattgat tcaaatagta gatttataga
    61141 ttctgataca agaaatactg gcaaacgata gttaaggaat aaagactaaa cttaaactca
    61201 aaaagaggtc atgcgaaaga ataaatcgca tgacctctta ctatattttg aatgtgttta
    61261 aacgttcaaa taatgaatct tagtaccaac cgtttgcttc ccagaatgct ttcgccgctt
    61321 cccaagaacc ataacgtgaa gtaacatatt gttctgctac tctttcttgg tttgcagcag
    61381 agtagtcacc atttaagtaa gaagaatcta attggtaacg gccgatgtaa cgtccgttcg
    61441 ttgctgtgta agaaccgctt gattcttttt gtgcgatcca ttcttttgca gagcttgtac
    61501 ttgctggagt agcagcagtt tctgttacag caggagcagg ttgtgtttca gcaacaggcg
    61561 ctgcttgtgt ttccgtttga accgtttctt gtacaacagg ttgttcagct tgaacaggtt
    61621 cagcttggac tggtgcttct tgaacggctg cttgaggttc agcagctgct tgtgcagagc
    61681 cttctgttgg gattgttaat tgttgtcctg agta
//
LOCUS       DAZUMM010000005        61277 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000005 DAZUMM010000000
VERSION     DAZUMM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.005"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            171..1643
                     /locus_tag="ACG8E6_000253"
     CDS             171..1643
                     /locus_tag="ACG8E6_000253"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFS5060706.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            1655..2479
                     /gene="nadE"
                     /locus_tag="ACG8E6_000254"
     CDS             1655..2479
                     /gene="nadE"
                     /locus_tag="ACG8E6_000254"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFS5060707.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(2552..3442)
                     /locus_tag="ACG8E6_000255"
     CDS             complement(2552..3442)
                     /locus_tag="ACG8E6_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFS5060708.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            3640..5157
                     /locus_tag="ACG8E6_000256"
     CDS             3640..5157
                     /locus_tag="ACG8E6_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFS5060709.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            5129..6418
                     /locus_tag="ACG8E6_000257"
     CDS             5129..6418
                     /locus_tag="ACG8E6_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5060710.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            6593..7489
                     /locus_tag="ACG8E6_000258"
     CDS             6593..7489
                     /locus_tag="ACG8E6_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5060711.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            7503..8366
                     /locus_tag="ACG8E6_000259"
     CDS             7503..8366
                     /locus_tag="ACG8E6_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5060712.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            8372..8935
                     /locus_tag="ACG8E6_000260"
     CDS             8372..8935
                     /locus_tag="ACG8E6_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5060713.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            8957..10468
                     /locus_tag="ACG8E6_000261"
     CDS             8957..10468
                     /locus_tag="ACG8E6_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFS5060714.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            10480..11439
                     /locus_tag="ACG8E6_000262"
     CDS             10480..11439
                     /locus_tag="ACG8E6_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS5060715.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            11525..12604
                     /locus_tag="ACG8E6_000263"
     CDS             11525..12604
                     /locus_tag="ACG8E6_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5060716.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(12856..14256)
                     /locus_tag="ACG8E6_000264"
     CDS             complement(12856..14256)
                     /locus_tag="ACG8E6_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFS5060717.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            14474..16135
                     /locus_tag="ACG8E6_000265"
     CDS             14474..16135
                     /locus_tag="ACG8E6_000265"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFS5060718.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16092..16790
                     /locus_tag="ACG8E6_000266"
     CDS             16092..16790
                     /locus_tag="ACG8E6_000266"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS5060719.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            16806..18230
                     /gene="araA"
                     /locus_tag="ACG8E6_000267"
     CDS             16806..18230
                     /gene="araA"
                     /locus_tag="ACG8E6_000267"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFS5060720.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            18537..19696
                     /locus_tag="ACG8E6_000268"
                     /pseudo
     CDS             18537..19696
                     /locus_tag="ACG8E6_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(19673..19813)
                     /locus_tag="ACG8E6_000269"
     CDS             complement(19673..19813)
                     /locus_tag="ACG8E6_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFS5060721.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            19814..21673
                     /locus_tag="ACG8E6_000270"
     CDS             19814..21673
                     /locus_tag="ACG8E6_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5060722.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            21783..22217
                     /locus_tag="ACG8E6_000271"
     CDS             21783..22217
                     /locus_tag="ACG8E6_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060723.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22476..22637)
                     /locus_tag="ACG8E6_000272"
     CDS             complement(22476..22637)
                     /locus_tag="ACG8E6_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060724.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22656..23678)
                     /locus_tag="ACG8E6_000273"
     CDS             complement(22656..23678)
                     /locus_tag="ACG8E6_000273"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFS5060725.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            23806..24159
                     /locus_tag="ACG8E6_000274"
     CDS             23806..24159
                     /locus_tag="ACG8E6_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS5060726.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            24171..25337
                     /locus_tag="ACG8E6_000275"
     CDS             24171..25337
                     /locus_tag="ACG8E6_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5060727.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(25429..26049)
                     /locus_tag="ACG8E6_000276"
     CDS             complement(25429..26049)
                     /locus_tag="ACG8E6_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS5060728.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(26241..26537)
                     /locus_tag="ACG8E6_000277"
     CDS             complement(26241..26537)
                     /locus_tag="ACG8E6_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS5060729.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            26741..27391
                     /locus_tag="ACG8E6_000278"
     CDS             26741..27391
                     /locus_tag="ACG8E6_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFS5060730.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            27407..29134
                     /locus_tag="ACG8E6_000279"
     CDS             27407..29134
                     /locus_tag="ACG8E6_000279"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5060731.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            29131..31023
                     /locus_tag="ACG8E6_000280"
     CDS             29131..31023
                     /locus_tag="ACG8E6_000280"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5060732.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            31727..32596
                     /locus_tag="ACG8E6_000281"
     CDS             31727..32596
                     /locus_tag="ACG8E6_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5060733.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            32805..33830
                     /locus_tag="ACG8E6_000282"
     CDS             32805..33830
                     /locus_tag="ACG8E6_000282"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS5060734.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            33869..34819
                     /gene="manA"
                     /locus_tag="ACG8E6_000283"
     CDS             33869..34819
                     /gene="manA"
                     /locus_tag="ACG8E6_000283"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFS5060735.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            34913..36616
                     /locus_tag="ACG8E6_000284"
     CDS             34913..36616
                     /locus_tag="ACG8E6_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5060736.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            36618..38651
                     /locus_tag="ACG8E6_000285"
     CDS             36618..38651
                     /locus_tag="ACG8E6_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFS5060737.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            38662..39102
                     /locus_tag="ACG8E6_000286"
     CDS             38662..39102
                     /locus_tag="ACG8E6_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5060738.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(39302..40429)
                     /locus_tag="ACG8E6_000287"
     CDS             complement(39302..40429)
                     /locus_tag="ACG8E6_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5060739.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(40432..41109)
                     /locus_tag="ACG8E6_000288"
     CDS             complement(40432..41109)
                     /locus_tag="ACG8E6_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5060740.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            41373..43139
                     /locus_tag="ACG8E6_000289"
     CDS             41373..43139
                     /locus_tag="ACG8E6_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFS5060741.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            43154..43591
                     /locus_tag="ACG8E6_000290"
     CDS             43154..43591
                     /locus_tag="ACG8E6_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5060742.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            43604..44755
                     /locus_tag="ACG8E6_000291"
     CDS             43604..44755
                     /locus_tag="ACG8E6_000291"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFS5060743.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAEFIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(44817..45515)
                     /locus_tag="ACG8E6_000292"
     CDS             complement(44817..45515)
                     /locus_tag="ACG8E6_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFS5060744.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(45508..46860)
                     /locus_tag="ACG8E6_000293"
     CDS             complement(45508..46860)
                     /locus_tag="ACG8E6_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFS5060745.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(47083..48483)
                     /locus_tag="ACG8E6_000294"
     CDS             complement(47083..48483)
                     /locus_tag="ACG8E6_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS5060746.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            48644..49381
                     /locus_tag="ACG8E6_000295"
     CDS             48644..49381
                     /locus_tag="ACG8E6_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFS5060747.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            49506..49985
                     /locus_tag="ACG8E6_000296"
     CDS             49506..49985
                     /locus_tag="ACG8E6_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFS5060748.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            49982..50749
                     /locus_tag="ACG8E6_000297"
     CDS             49982..50749
                     /locus_tag="ACG8E6_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFS5060749.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            50979..51548
                     /gene="lepB"
                     /locus_tag="ACG8E6_000298"
     CDS             50979..51548
                     /gene="lepB"
                     /locus_tag="ACG8E6_000298"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS5060750.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(51652..52254)
                     /locus_tag="ACG8E6_000299"
     CDS             complement(51652..52254)
                     /locus_tag="ACG8E6_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS5060751.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            52360..52590
                     /locus_tag="ACG8E6_000300"
     CDS             52360..52590
                     /locus_tag="ACG8E6_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060752.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            52581..52829
                     /locus_tag="ACG8E6_000301"
     CDS             52581..52829
                     /locus_tag="ACG8E6_000301"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5060753.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            52935..53519
                     /locus_tag="ACG8E6_000302"
     CDS             52935..53519
                     /locus_tag="ACG8E6_000302"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS5060754.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            53522..54595
                     /gene="prfA"
                     /locus_tag="ACG8E6_000303"
     CDS             53522..54595
                     /gene="prfA"
                     /locus_tag="ACG8E6_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFS5060755.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            54588..55427
                     /gene="prmC"
                     /locus_tag="ACG8E6_000304"
     CDS             54588..55427
                     /gene="prmC"
                     /locus_tag="ACG8E6_000304"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFS5060756.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            55420..55599
                     /locus_tag="ACG8E6_000305"
     CDS             55420..55599
                     /locus_tag="ACG8E6_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060757.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            55621..56646
                     /locus_tag="ACG8E6_000306"
     CDS             55621..56646
                     /locus_tag="ACG8E6_000306"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFS5060758.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            56821..58065
                     /gene="glyA"
                     /locus_tag="ACG8E6_000307"
     CDS             56821..58065
                     /gene="glyA"
                     /locus_tag="ACG8E6_000307"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFS5060759.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            58218..59165
                     /locus_tag="ACG8E6_000308"
     CDS             58218..59165
                     /locus_tag="ACG8E6_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFS5060760.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            59299..59928
                     /gene="upp"
                     /locus_tag="ACG8E6_000309"
     CDS             59299..59928
                     /gene="upp"
                     /locus_tag="ACG8E6_000309"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFS5060761.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(60067..60309)
                     /locus_tag="ACG8E6_000310"
     CDS             complement(60067..60309)
                     /locus_tag="ACG8E6_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060762.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            60430..60502
                     /locus_tag="ACG8E6_000311"
     tRNA            60430..60502
                     /locus_tag="ACG8E6_000311"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60463..60465,aa:Thr,seq:cgt)
     gene            complement(60621..61259)
                     /locus_tag="ACG8E6_000312"
     CDS             complement(60621..61259)
                     /locus_tag="ACG8E6_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFS5060763.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT        20352 a        10733 c        12673 g        17519 t
ORIGIN      
        1 aggttaaata ccaataagaa aggatagttt tagcagatgg agttcatttt tagcaagaaa
       61 tatccgtaaa acatgtgctt tttcagaaaa aatggaagat ttcttttcct attaattttt
      121 tttgttataa tgggtaggaa atgaaaactt gccaagagga gtaatacaag atgaataata
      181 cttacccaga cgacagtttg acgctgcata cggatctata tcaaatcaat atgatgcaga
      241 catactggga attaggtcga gctgatttac atgctgtttt tgaatgttac tttcgagaaa
      301 tgccttttaa ccacggatac gcggtttttg caggattgga acggttagtc aattatttag
      361 agaatctgac gttcaatgat tccgatattg cttacttgcg tggattagaa gtctatcctg
      421 aaggtttttt ggagtattta caaaattttg aatttaaagc aacagttcgt tctgctcgtg
      481 aaggagaact agtttttgca aatgaaccat taatccaagt ggaagggcct ttagcgcact
      541 gccaattagt agaaacagca ctgttgaata tggtcaattt ccaaacgttg atcgcgacaa
      601 aagcagctcg tatcaaatct gtcatcggag aagatccact attggaattt ggaactagac
      661 gggcacaaga actggatgca gcagtctggg gaactagagc ggcatatatc ggcggagcag
      721 atgcaacaag caatgtacgt gctgggaaga tatttgggat accagctagc gggacccatg
      781 ctcattcgtt agtccaatct tatggaaatg attatgaagc atttatggcc tacgctaaaa
      841 cccataaaga ctgcgtgttc ttagtcgaca cttatgatac gttgaaatca ggtgtgccaa
      901 gtgcgattcg cgtggcgaaa gaattaggtg acaaaatcaa cttccaaggc gtacgcatcg
      961 acagtggaga tatggcctat atatctaagc gtgttcggga acaattagat gcagccggat
     1021 ttacggaagc aaaaatctat gcttcgaatg acttggatga agcaacgatt ttgaatttaa
     1081 agatgcaaaa agctaaaatt gatgtatggg gtgttgggac aaaattgatc acagcctatg
     1141 atcagccagc ccttggtgca gtattcaagc tagtatcgat cgaagatgat gaaggaaaaa
     1201 tgatggatac gatcaagtta tcaagcaatg cagaaaaagt aactactcca gggaaaaaac
     1261 aagtgtggcg catcactcga aattttgatg ggaaatctga aggagactac gtgacattat
     1321 gggatgaaga ccctcgagaa gaagaagcta tcttcatgtt ccatccagtg catacgttta
     1381 tcaataaaac tgttcgagac tttacagctc gcccagtttt acaagatatc tttatcgaag
     1441 gaaaaagagt ttacgaatta cctgcactaa acgaaatcaa agaatataca aaagaaaatc
     1501 tagattcact atgggaagag tataaacgag acttgaatcc gcaaaaatac ccagtcgatc
     1561 tgtctactga atgctggaac cataaaatgg ctatcatgga acgaatgaaa aaaagcgtag
     1621 cagaacttca tacagaagca taggaggaga ataaatgagc ctgcagacag aaattatcaa
     1681 tgaattaggt gtcaaaccaa caattgatcc aaaagaagaa atacgtaaaa gtattgactt
     1741 tatgaaagca tatttgaaaa aatatccgtt tcttaaaact tttgtactag gtatcagcgg
     1801 cggccaagat tctactttag caggccgttt ggcgcaacta acgatggaag aaatgcgtga
     1861 agaaacaaaa gatgaaaact atcaatttat cgctgttcgt cttccttatg gtgaacaagc
     1921 agatgaagaa gatgcaaaag ctgctcttga ctttatccag cccgatgtta gtctgcgagt
     1981 aaatatcaaa ccagctgttg atgcacaagt acaagtatta agcgaagcag gtgtgggaat
     2041 cagcgatttt aacaaaggaa atatcaaagc acgtcaacgt atgattacgc aatatgccgt
     2101 agctggagaa agagcaggag ccgtattagg gacagatcat gctgcagaaa acattactgg
     2161 attcttcacg aaatttggtg atggtggagc cgacattttg ccattattcc gtttggacaa
     2221 acgacaaggg aaacaattgc ttcaagcatt gaatgcaccg gaaaaattat acacaaaaat
     2281 tccgactgca gatcttgaag atgggaaacc aatgattgca gatgaagtcg cactaggtgt
     2341 cacatataat gaaattgatg attatctaga aggaaaagaa gttcccgctc aagcacaaga
     2401 aaaaatcgaa gcatggtgga acaagacaca acataaacga catttgccaa tttccgtttt
     2461 ggatgatttc tggaaataac aataaagcaa aaacagccgt cccttcatag ggattggctg
     2521 tttttgtttt ataaaataga aaagaaaaat atcagctgta gtaaactttt aatttgatat
     2581 cctgatcgta attaccgaat cctttaccaa ataaagtaca gccccccaca tgtttggcat
     2641 cgtctttcac ttcgattcgc aaatgccaag tgtcttgctc tttagggatg tcgttgatcg
     2701 tgatgtttga caagggttct ccattcatat tcgtcaagtg tttggtaatt cggatcgtct
     2761 tcagcaagcc gtattgatta agattatctg gataccaatc aggcgtatat ttaccgcgga
     2821 tatcagcaaa atctccaggg ctcgtccatg ttcctagctc cacaccattt aggctgaaag
     2881 tgatatctga aggccaattg tcatttgaaa aaggaaattc agaagaaatt tccacagaaa
     2941 cttccaacat ctccaatgta tcctctgtat ttaaaaaatt aggtgcctga tactcgacaa
     3001 atccgctggt aaaccagaga atgcgggcat ccatccttcg aggatccata aaatagcgtg
     3061 gttcatccac tttcccgata aaatcatgga tcgtagctaa accgcatgtt ggttcaattg
     3121 cataatttgt atagtgacca ataggtatcg atgtttcttt cgtatcaaaa gcattgaata
     3181 ttttttctgg aaaactgata tcgattttat cgacacgaag gctcgaaatt ttttgttgtc
     3241 ctactttttc tgttttgatg atgtttgctt cctctagctt tttcacgtgc atcgtcgtga
     3301 tagcactgct cactccaagt tcttttgcca aatctttgac attcatcttt tttttggaaa
     3361 gtagctggat gatcttgatc cttgttttac tagctaatgc ttcatatacc ggtaaggatt
     3421 cttcgtttat ttgtagttgc atatatatca cctcttaaaa ggtttttctt atatattaac
     3481 atatttgtta ataaaagtta tattaaatta tttgttttaa tcattattaa tattttggtt
     3541 aattaaaatc gataaaaaat aagtaagttt aacatttata ttattgacaa cgctttcaac
     3601 gaggtgtatt attcatgtat aaattaaagg aggtcagtta tggaagccac aatgttgatt
     3661 agtagtgtca agcgtgtagg caaagttgat ccgcggttgt acagttcatt tattgaacat
     3721 atgggacgag cagtatatga agggatttat cagccagatc atccatcagc taataaaagt
     3781 ggatttcgcc aagacgtcat tcacttggta aaagaattga acattcccat gattagatat
     3841 cctggtggaa attttgtgtc aggatttcgt tgggaagaca gtattgggcc catagcaaat
     3901 agacctagaa ggttggattt agcttggcgg acaattgaaa cgaatgaagt tgggattcat
     3961 gaattttata actggtgtca gcatgtgggg gccgaaatca atatggctgt caatctgggt
     4021 actcgtggag tagatgcagc tcgtaattta gtcgaatatt gtaattttcc taaaggaact
     4081 tattggagtg atttaaggcg ttcgaatggt caagagagcc cttttggaat taagacatgg
     4141 tgtttaggga atgaaatgga cggtccttgg cagattggac ataaaacagc ggaagaatat
     4201 ggacggttag cagaggaaac ggctaaggcg atgaaactcg tagatgattc aattgaggtg
     4261 gttttatgcg gaagttcgaa tcggcaaatg cctacatttg gtgattggga gttaacagtt
     4321 cttgatcaag cgtacgatca aatagattat ttgtctctgc atcaatatta tggcaaccaa
     4381 aaaaatgatc tttctcatta tcttgcacgt tccttagaca tggatcggtt catttctgaa
     4441 gtgacagcga tgtgcgatac agtcaaggcg aagaaacata gtaagaagca gatcaacctg
     4501 tcctttgatg aatggaatat ttggtatcat tcagcggaac aagatgagca agcagcgcct
     4561 tggcagattg ctcctccttt actagaagat aactataatt ttgaagatgc attactatta
     4621 ggctgtctat taatcacttt attgaaacat gcagatagag taaagattgc ttgtcttgct
     4681 caattggtaa atgttattgc accaatcagg acagaaacaa acggagaagc ttggagacag
     4741 acgatctatt atccatttat gcaagctgct gtgtatggac aaggagaggt gttaagccca
     4801 caaattcgtt cagcaaacta tgcgacggaa gattttgaag aagtgccgta tttagaaagt
     4861 atagccgtaa taaatgacaa agaacttgtc atatttgcag ttaaccgtgg agaagaagaa
     4921 atgaatctaa ctgttcactt gtctgaatta tcattagagg gagtgattga tttttctgaa
     4981 atgagtggat ttgatacaaa agcaactaat cttcctaaaa gtgaacaagt aaaaccaagt
     5041 cattcaacaa atgtaaaagc tgaagatggg aaaatcaatt gtttattgaa accgctatct
     5101 tggaatgtgg tcagatgtcg tttatcagat gaaggaggaa caaaaaatga aaggtaaaaa
     5161 atggttaatt ggctctttgt tggtagctgc aacaggtatt ttaggagctt gcggtggcgg
     5221 aagttcagca gaatctgatt ctgataaaga agtgactttg actttttgga atgggtttac
     5281 tgcatctgat ggtgaaatat tgcaagaaat cgttaatgaa tttaataaaa caaatgataa
     5341 aaacatcaaa attgaaatgg atatcatgac ttgggccaat ttgaatgaaa aacttcctac
     5401 agcgatttct tctaaaaaag cacctgactt tattgcatta aattatccag attttgcaca
     5461 gtatgtagaa aacggagcgg tacaaccatt agacgatttc tgggaatatg atggagtaga
     5521 taaatctgat tttaaagaaa cggctattga attaggtgaa gtagaaggta aacagtattt
     5581 tgtccccatg caggtacaag gcatgtatat gtattggaat aatgatttgt ttaaacaagc
     5641 tggattagat actgaacaac cacctactac ttgggaagaa ttgacagaaa tggcaccaaa
     5701 attaactgat agtagtaaaa atgtatcagg ttttgtcttc aataaggatg gaactgcacc
     5761 gttatataac tggatgttag caaatggcgg taaattagtg aacgatgatt atactaagtc
     5821 tgaatttgcg tctcctgaga accttaaaac gttaaaagcg atccaaaaaa tgatccatgt
     5881 agacaagact ggtccagaat cgatttctgg cgccgaaatg gacaatttaa tgaatgctgg
     5941 tcaactagcc attgaaatca atgggccatg gttaaataac ggattgaaag caaacgagat
     6001 tgattataat gtgactacct taccacaagg gagtgattca gacaaaaaaa cagctatttt
     6061 agatggcgta ggatatgcca ttccggcaag tacagataat aataaaaaag aagctgtcta
     6121 tgagtttttg aaatattgga atacaactga aattggtaaa aaatggagta tcgaaaatgg
     6181 ttttcctgcc tacttggatt cagtagcaaa agatgaagaa gtaaaaaatg atcctattgt
     6241 atctgaatta tcgaaacaaa tggactatgc agagccatat ttaccaggtt ttacccaaat
     6301 ctctgcgatc aataacgata tcatcaatcc actgattgaa aaattattag caggagaaga
     6361 tcctgagaca ctcatgaacg atgcagatga tgccatcaat caattactgc aaaaatagta
     6421 cagaacttta tacaggatag actgttggca agatacagat atctgaatga tcgacctagt
     6481 atttgcagat aggtgttatt gcaaaaatca cagcgattgt tcggatatct tattcagatt
     6541 cgtttaaacc aagattgtat attttatcaa aatgaaagat gaggtggact atatgaacgg
     6601 attcaccaaa tattggaatc gtcctgatcg tgctgcgtat ttgtttttaa ctccatcttt
     6661 tttagtttta ttattattta ctgtcatacc gcttttagga acatttgcta ttagttttac
     6721 agatttgaat attttcttta catcaagaaa ttttgtaagt tttgaaaatt tcacacgtat
     6781 atttagtgat gaacgagcag ttaattcgtt gattcattct ttatatttca ccgtactcga
     6841 aacgccaaca caaattatcg ttggtttatt agcagcagta gcattatcta aaaataccag
     6901 attcaatcgt ttttgtcgtt cgacatttta cattccagtt atttgttcat tgacgtcaat
     6961 tgcgattatt ttttctatgt tgcttgatcc gaatgtaggg gcgattcctt acttgttttc
     7021 acaagtcggc ttgccgatcc ctcaattttt tcgcgatcca acccttgcta tgccgacggt
     7081 agccatcatg actgtttgga agaattttgg tgttactatg acgattctct tgactgctat
     7141 tcagggaatc tctccctctt tgtatgaatc tgctcaaatg gatggtgcaa caaatcgaca
     7201 gcagtttttt aacattacgc tacctcagat cgttccctcc ttaggttttt gtattttaac
     7261 gaatctgatt ggttcgatgc aagtgtttga ccaagtatat gttgcaaccg ctggtggccc
     7321 acaatttaag acagaaacag ctgttcaata tatttacagt cgaggattta cagcacctta
     7381 tgaattgggc tatgcatcag cactgtcttc ggttttgttt atcattgttg caactatagc
     7441 tatctccact aatgtgtaca tgtcacggaa agaaaaacaa atgaaatagg agggaaacct
     7501 gtatggcacc agaaagctcg attacagtta ctgtgaatag tcgaaaagca gttagtggta
     7561 aaaaaatcat tcgaacgatt agtatgctag ttatattgtt ggttattgcg tttccttttt
     7621 tgtggctgat tatttcttct tttaaacacg aaaaagacat tatctcattt ccgcctagaa
     7681 tatttgcaga tagctatacc ctagataact atattaaggt ttggacaacg atccctttat
     7741 tagattacat caaaaataca gtgatttttg ctggagggac agtgatcact tctgtattct
     7801 ttgattctct tgcggggtat gcatttgctc gtatgcgttt caaaggaaaa tcagttttgt
     7861 tctatttcgt tttgctgaca atgatgatcc cattccaagt cttcatgatt cctctattta
     7921 ttgaagtcaa tttgcttggc atgcttgata cttatgcagg tttgatcatt cctcgtatga
     7981 caactgcatt tggtatcttc atgatgcgtt ccttttttat tactttacca gattctttgg
     8041 aagaggcagc acgcattgat ggattaagtg agtttaatat ttttttgaaa atcatgttgc
     8101 cattgagcaa acctactctt ttatcattag ggattttcac gctgatgaat agttggaatg
     8161 acttgcttta tccattgata ttgacaagta gttcaaaaat gagaacttta ccagcagggt
     8221 tagctttatt tacagggcag aacatttctt tttatggacc tgtaatggct ggaacggtta
     8281 tttcaatgct gccattacta gtggtttata tttttgcaca gaaatatttt gttcaaggaa
     8341 cagctatgtc tggaatgaaa gagtgaggaa gatgaaaata gttgagacta aactttatgg
     8401 acagttgatt gtggtttctg attacatctt attaggtatt ctctgggtaa tcgtgtgttt
     8461 gcctgttatt actatcgtgc ctgctacatg tgctgttttt tatgtaatgg cgcaatggaa
     8521 aggtgaggga tcaggaaata tcatttattt tttctttcaa ggaataaagc aacattttgg
     8581 taagaacttg ttccttagtc tgttaacgat cattatttat tttcttgtta atcaattatt
     8641 agaagaacag acaatcgtac tttcaattag tggatatgta gttttactta tttattccat
     8701 gtttctttta tcctggatat atgtgatttc tgaaaacaaa gatatcacat ttttacaaat
     8761 ttttgaacaa agtgcatgga aagtattttt tgattttttg agaaatattc tttgttgcgt
     8821 attgttatta ggatttgtac tgctcatatt tcttttccct ccttttattt ttatctttgc
     8881 tggtggcgta tggaaacttg tatctattat cttgactata ggaaatgaaa ggtagggaaa
     8941 gctgttggac gaagaagtga aagtaaaaat cagagaggat tttacggtag gagagatcag
     9001 ttctcggctg tttggctcat ttgttgagca tatgggaagt gtcgtctata cagggattta
     9061 tgaaccgggc catgaaacag ctgatggtga cgggtttaga caagatgtcc tctcttatgt
     9121 aaaggaatta gaattaggtg tcattcgcta tcctggagga aactttacat ctggttatga
     9181 ttgggtggat actgtaggta ggaaagaaca acgaccaaaa aaaattgatt tagcatggcg
     9241 aggtatagaa ccaaattctt ttggactcca cgaatttttt aaatggatca gtcatgtaca
     9301 agcgaaaccg atattcacgg tgaatttagg aacaaaagga attgatgaag caaaaaattt
     9361 agtagaatat tgtaattttc caaaaggaac cttctggagt gatttaagaa taaaaaatgg
     9421 gcaaaaaaag ccttttggcg ttaaattatg gtgtttaggg aatgagctag acggtccatg
     9481 gcaagtgggg gctaagagtg cttatgaata tggtaggtta gcaaatgaag cctcaaaagt
     9541 catgaagtta gtggatgatt caattgaaac aatcttagtt ggaagctcta cgccacgttt
     9601 accatcgtac cctgaatggg atcgtatcgc attagaagaa gcttatgaga atgtagatta
     9661 tttagctttg cataactata ttgataagta tgatgaagaa gacttgacta aacctcctaa
     9721 aaatgagcgt gatgatacag cgacatacct tgcaaaagct tatcgattcg atcgtcaaat
     9781 tgaagaaatc gttgctactt gtgattatgt aaaaggatat ttgagaagtg agaaaacaat
     9841 gtatctagcg tttgatgaat ggaatgtaca tgcccaaccg gaaaaaagac atagggattt
     9901 tgaagtcggt agtcccatcg attggtgtta ctttactatg gaagatacat tattatttgg
     9961 ttcattaggg ctggctattt tacgacacgc agatcgcgta aagattagtt gtcaatcatt
    10021 gttagtaaac acaatacctt taatactgac agatgctaaa ggacaagcat ggaggaatcc
    10081 aacttattat gtgatgcaac acttatcgaa atatgctaaa ggtcaagtat tgaaacaaca
    10141 aatcatttgc cctgtttatg attgtgaaaa gataaaaaat gttccttatg tggacagtgt
    10201 gatcgtagat aacggcaatc agctgactat tttcttgatc aatcgcacaa atgaacaaca
    10261 aaagataacg attgaacatg atttcaattt gctagatcaa ggagagcact acacattaac
    10321 cagtagcgat ctgttggaca aaaatacacg tgaagaacca gaaaaaataa aacctgaaat
    10381 aaactatttt tcaatagaaa aacatgacga attaaatgta gggatagaaa aatactcttg
    10441 gaatgtaatt agaataaaga aaatgtgagg aaatttgtta tgatgaagac actttataat
    10501 aatcctatcg ttcggcaacg ggcagatcct tgggtatata aacatacaga tggttattat
    10561 tattttacag gatcggttcc aggctaccaa gtgattgaat taagaagggc aaagtcactg
    10621 aatgaattag aacatgctgc aagcctgatt gtatggcagg ctcatgaaga aggaccgatg
    10681 agtgaattga tctgggcacc tgaagtccat tatatcaatg gaaaatggta tatttacttt
    10741 gctgcaagca atcataaaac tatacgtgat gaatctcatc atcaccgcat gttcgtactt
    10801 gaaaatcagc atatagatcc aatgaataca gattggatag aaaagggaca aataaaaacg
    10861 aaaagggaaa ctttcagttt agacggaaca actttctctt ctcaaggaaa gctatattat
    10921 gtgtgggccc aacaagatcc tgaaattact gggaattcta atctttatct atccgaaatg
    10981 gataatcctt ggacgttaaa ggggaagcag atcctccttt ctatacctga gtatgaatgg
    11041 gaaaaacggg gtttttcagt aaatgaaggt ccagcagtcc ttactcgcaa tgggaaaata
    11101 ttcattacgt attctggtag tgcaacagat gaaaactatg ccatgggcct gctgtgggct
    11161 gatgagcaca agaatctttt attaaaagaa tcatggcata aattatcgga gccagtattc
    11221 gtttcttctg aaaaaaataa gcaatttggt cctggtcaca actcgtttac ggtttcagaa
    11281 gacgggtgct ttgatatttt gatctatcat gctcgaccag agaagaacca aaaaggggat
    11341 ccgttagaca atcctaatcg gcacgcgaac gcgcaatgct ttacgtggaa tgagcaagga
    11401 tttccagaat ttggtgaacc tgttccgtat gccttatgag cacaagaaac cttgattttt
    11461 gcttgaataa accttgcaga ctcgttatga tatatgtacg tacaaataaa aaggggcaag
    11521 gcaaatggca aaatatcaag atattgcaga agagataaaa caaaagatcc ttcgtgagga
    11581 atacgaagta aatacaacca ttccctcaga attcaagctg caagaaatat atgacgtcag
    11641 cagacatacg attcggcaag caatcgctgt gttggtaaat gagggctatc tgcgtaaaga
    11701 aaaagggtcg ggcacatatg tagatgacag ctataagaaa gaaaaaacac cattgaacag
    11761 aacaaaaacg atcggtgtca tcaccacata tctctctgat tatatttttc cgtcgattat
    11821 ccgaggaatc gaaaatgaat tacgagaatc tgggtattca ttgcttttag ccagtacgaa
    11881 taacgatcac gaattggaga aagagtgttt gaagaaaatg attgcttatg gggtagatgg
    11941 tttgatcgtt gagcctacga aaagtaatca gtataaccca aacttagcac tttatgttgc
    12001 tttgagagaa caaggcattc cgatggtgat gatcaatgct gcttatgaag agttaaatgc
    12061 ctcttatatt tgtttagatg atgtgaaagt cggttttatg gcagcagaat atttgataaa
    12121 aaaaggacat cagcgtctgt tgttcatcac aaaaatcgac gatcttcaag gaaaatacag
    12181 gatgaaaggt tttatcagcg cgtgtgaaca gtataatgtg cgttttttga cggaagatat
    12241 cattactttc accacggaaa cacgagaaac aatcgctgaa aaaacagtac aacggttgaa
    12301 agaagaacaa gctttagatg gcatcatttg ctataacgat caaatagcta gtttaatgct
    12361 agataagttg acagaaaatg gctatcatat tccagatgac ctatcgatcg taggaacaga
    12421 cgattcttct ttaagccatt tagggaataa aaaattgaca acattgacgc atccaaaaga
    12481 aaagttggga caagatgccg cacggtggat cgtcacaacg attgaaaaag gacaggcaca
    12541 gccaaatatt ctctatgaac ctgtactagt tgaacgggaa acagtgaaag aaaacgaaaa
    12601 ataagagacg ggtttttacc gcctcttatt gctcctacgg ttactcgtcg caatgtttgt
    12661 gagagctttt gtcatcgctc aaggctgaac gtttctttca ccacctctcc actaaacggt
    12721 gttctcctac caactggtca caagtcacaa tattcatcaa acatgagaag ctgttttttg
    12781 aaattgttcc ttgtgactgg aaactaagcg gttccttctt atttcttgaa gctgaccact
    12841 tctgtcacaa cctctttaag aatgatggat agaaatacgt ttaggataag tttttttaga
    12901 tgcactttgt tcgatttcct ctaaagattt cccacgagtt tctggtacga tctcttgaat
    12961 aaataaaacc cctaaaaagc agatgatccc aaaaatgccg aaaacgattt cttgagacat
    13021 agaagcagtc atcacaggaa aaagcaagcc aactaggaaa gaaccaatcc aattaaaaga
    13081 agaggctagg ccagatgctc gcccgcggac agctagtggg aatatttcgc ctacgattac
    13141 ccatgtcagt ggagcccatg tgcaagaata aaatgcgaca taaatacata aaaacaagag
    13201 aatcaacaac gaatttgtat gagcatccag tacagttcct aaaacagctg gtaggatgaa
    13261 agaaagtccc atcacgctac cgcctatttt caataaagtt cgccgattga acttgtctgc
    13321 aataaccaaa aagagcaaag aaccagcaac taaaatgatt ccttggatga tcggccacat
    13381 caatgcatcg ctcgctgcat gcccagtcgc tttttctacg atcagcggaa tatagtaaaa
    13441 aatagcatta gctccttgaa attgctggaa tactgctaca ccaataccgg caatgactaa
    13501 ggaacgatat ttttctaaaa acaaagtatg ccaagaagtt ccggttgtct tttcttgttt
    13561 gacattcagc tggatttgtt cgaattcctg ttcagcttct tttggtgtcc gaatatagtt
    13621 caatactctt tttgcctctt ccaagcggcc agattgcata agaaaacgcg gtgactctgg
    13681 tagagctaaa acaccgaaaa ataaaatcag cgcggggaca gcagcgagcc ctaacatcac
    13741 gcgccaagcc atcgtttcag gcaacccttt tagcaaataa tctgcaacat aggaaaggag
    13801 catgccagaa gcaatcatca cttgattgat ccctgacaat cttcccctta gtctagcagg
    13861 agccatttca gacatataag caggaactag ggcagatgca gcgccaacag ctaaacctag
    13921 aagaatcctt gctgcaatca gaaaataaga accattgtga ggggcagatg cagaaaggac
    13981 agaaccagcc ataaaaataa gtgcagaaag aagaatcatt tttcgtcgtc caagcttatc
    14041 agaaagaaga ccagataaag agccgccaag gatcgcacct aacatgaccg aagacgtgat
    14101 ccatcctatc aaagaagctt tacctgccaa accccaatca tgctgtaaaa aaggtaaggc
    14161 gccggtcata acaccaatat catatccaaa tagaatgccg ccaaatgcgc caaaaaaata
    14221 aataaacgat gaggaaactt ttttttcttt tttcataagt attctctcct tctagaattt
    14281 cgagaaggcg gttgatcaga ttttgtaagc aatttcattt tataacttat gtacgtacaa
    14341 aacaagtgtt ttttattgta ttcttgtaaa taactgcttg acatgtacgt acaaaagtat
    14401 acaataatca ttgtaagcgc tactaaaaag acggtttgat caggtttgac gagaaggagg
    14461 gagttaaatc attatgaagg aagtaaatgc gcaaagtatg ttagatgtca tagatggtcg
    14521 tacatcttta gggattgagt ttggatcaac taggatcaaa gctgtattga ttgattcatc
    14581 ttttactcct attgccgctg gtagttatga gtgggaaaac aaactagaaa atggtatctg
    14641 gacctattca ttagaagata tttggaaagg actgcgcgtc agttaccgaa aaatggcagc
    14701 agaagtattc gaaatctatg gcgagaatct tacgacaatc gggtcgattg ggttcagtgc
    14761 aatgatgcac ggttatctag catttgatag caaggaacag ttactagtac catttcgtac
    14821 ttggcgaaat acaatcactg aagaagcaga acgaatatta acggaaaaat ttcaatacaa
    14881 tattccgcag agatggagta tcgctcattt gtatcaatct attttgaata aagaagaaca
    14941 tgtgaaagaa attaattttt tgacaacgct tgcaggttat gtccattggc agcttactgg
    15001 tgagaaagta ctaggcgtag gagatgcatc aggcatgttt cctatcgatc cttcaacgaa
    15061 aacctaccat caagaaatga aaaatatttt tggtcagtta atcgaaaaag cgcacgtagc
    15121 gattgacttg gatcacattt tgcctaaagt tctagtagca ggagaagaag ctggcaagtt
    15181 aagcgaatca ggggcttatc tgatcgaccc tacgggaaat ttgagaccag gaatcccggt
    15241 ttgtccgcca gaaggggatg caggaaccgg tatggtcgct acgaacagtg tagccaaagg
    15301 gacagggaac atttcggctg gtacttccgc cttcgctatg atcgtattag aaaaagaact
    15361 agaaaatgtc catccggaaa tcgacatggt cactacacct tcaggagaac tagtaggcat
    15421 ggttcatacg aataactgct cttcggatat caatgcttgg gtcagtattt ttgaagaatt
    15481 taccaattct ttgggtatgg agataagcag agataaatta ttcacggtat tattcaacaa
    15541 agcgctagaa ggcgatacgg attgcggggg attactttct tatggttatt tttccggtga
    15601 aaacattaca ggagtaaatg aaggaaggcc attatttgta cgtacaccag gtagccgttt
    15661 tgatttagcg aatttcatgc ggatccattt agccagtgct tttggtgcaa tgagaatcgg
    15721 aatggatata ttgaaatctg agaatgtcca aattgatcgt ttagtggggc atggggggat
    15781 ttttaaaaca ccagaagttg gacagcggat tctagcatct gctatggagg caccagttac
    15841 agtgatggac acagccggag aaggtggagc gtggggaatc gcgttattag cagcctatat
    15901 gatggatcga catgggaagt ctttagaaaa cttcttggca gaagacgtgt ttggacaaga
    15961 ccaaggaatc accatcacac cttcagcaga agaaatagca ggctatcaga tatttatgag
    16021 acgttaccgt gaggggatcg acattgaaaa aacagcgatt catcaattgc aagaaaaagg
    16081 agtgatagaa aatgctggaa caactgaaag aagaagtata ccaagcaaat cttgacttgc
    16141 ctaaacacgg actcgtcaaa tatacatggg gaaatgtgag cgcttttgat cctgaaacac
    16201 gatatttcgt tatcaaacct agcggagtca gttatgaaga gatgacagca gacgacatgg
    16261 tcgttgtgga cttggacaat catatcattg aaggtaaact caatccctct tcggatacgc
    16321 ctactcatgc tgttttatat cgttcatttc ctcaaatcgg aggaatcgtc catactcact
    16381 cgacatgggc gacgatctgg gcacaagcag gattggatgt tcctgcgatg gggacgacgc
    16441 atgcagatac tttttatgga tcggtacctt gtgctcgatt tttgacacaa caggaaatcg
    16501 acagcgggta cgaatatgaa acaggaaaag ttatcataga aacattcaaa gaacgcaaaa
    16561 ttgatccctt agcaattcca ggggtacttt tacacggaca cggtccattt acttggggga
    16621 aagatgcgca aagtgcggta atgaacgcag tcgtactaga tgaagtatgc aagatgaatt
    16681 tatttaccag acagctcaat tcattttcag aagagctgcc tcaacggatt ttagataaac
    16741 attatttacg aaaacacgga aaaaatgcgt attacggaca aaacaaataa aaggagtggc
    16801 taattatgtt gaatattgga gaaaaagaat tttggtttgt tgtaggatca cagcatttat
    16861 atggggaaga agcactgcga gaagtaaaga aacatgcaca agaaatggtc gatgaactga
    16921 atgaaaatgg ccaattgcct tatccaatcc gccttcaaga attagcagtt actgctgata
    16981 cgatcacaaa aatcatgaaa gaggtcaatt atcgagaaga agtagcaggt gtgatcactt
    17041 ggatgcatac tttctcacca gcaaaaatgt ggatccgcgg gacaaagctt ctacaaaaac
    17101 cattgcttca tttagctacg caatataacg aaagcattcc ttggaaaacg atcgatatgg
    17161 atttcatgaa tttaaatcaa tccgctcatg gtgaccgcga atatgggttc atcaatgctc
    17221 gattaaataa acaaaataaa atcgtcgttg gctactggaa acgacctgag atccaaaaag
    17281 agattgctga ttggatggat gttgcagtag cctataatga aagttttgga ataaaagtgg
    17341 ctcgttttgg tgacaacatg agaaacgtgg gtgtcacaga aggagacaaa gtggaagcac
    17401 agattcaatt tggttggact gttgattatt ttggtatcgg agatcttgta caagtaatcg
    17461 atcgcgtatc agacgaagaa gtcgatcaat tatttgaaga gtacaaggaa ttgtatacct
    17521 tcgactatgg tgattatgaa gaaaagacat gggaggaaca tgtaaaagta caagctcagc
    17581 aggaaatcgg tattcgccgt ttcttagaag aaggaggcta taacgctttt acgactaatt
    17641 ttgaagacct gtatggaatg aaacaactac ctggactagc tgtacaacgt ttgatggcag
    17701 aaggatacgg ttttgctggg gaaggcgact ggaaaactgc agcgattgat cggttgctga
    17761 aaatcatggc tcgagggaaa gatacaggat tcatggaaga ttatacttac gagctagcta
    17821 gtggccaaga agcaatcttg gaatcccata tgatggaagt agatccgacg cttgctgcga
    17881 cgaaaccaag aatcgttgtt tctcctttat caatgggaga tagagaagat ccggcgcgtc
    17941 ttgtatttga tgggaaagca ggagaagggg tagtcgtatc gatggcagat ttcggtacgc
    18001 attataaact gctgatcaat gaagtagaag cctttgaacc aacgactgaa gcacctaatt
    18061 tacctgttgc acgtgtgtta tggaaaacta aaccgaattt ccatgaaggt gtccattcat
    18121 ggattcaggc aggtggcggt catcataccg ttgtctcgct aaatctaaca acagatcaaa
    18181 ttgaaacgtg ggcaaaatta gttgagttgg aaacagtggt tattagataa aaagaggttg
    18241 tgaaaaagcc acttagtttt aagaaataag aagagattga cgaaatttgc ttttcaactt
    18301 ttttgaaatt tagtcttatt atcaatgtca gctaacggaa caccatttat caagatatct
    18361 caccctcaat attttgctct cgaaaataag gcttatggca gtaacttgtc atagccttat
    18421 ttttatcact cagcctactc tgctctaact gctctttttg cttctaggaa gtcaaatggt
    18481 aaacagttgt ctaattgcag aaatttcgag atacttgtta gtaaggaggg ggttttatgt
    18541 atcacgttca tttgattgat gctattccag aacaagagat agaaaagtta caaggggaat
    18601 atttcaaaat aatagatgat ttgccagccg aaggaatagt tattcgtagt acgaaagtga
    18661 aagatgaatg gcttacacca gatttgctag caatttcaag agctggtgtt ggggtgaaca
    18721 cgatcaacgt tgaaaaagct tctgaaaacg ggacgatcgt catgaataca ccaggtgtaa
    18781 atgctaatgc agtaaaagag cttgttttgt gctgtttgct cttatcttcc cgtccgataa
    18841 tcgaagcaag tcgtatggta caaacactaa caggtcctaa tattttagaa caggcagaaa
    18901 acaaaagaag tgcttatgtg ggacgagaat tgcaaggcaa aaccatcggt cttcttggtc
    18961 ttggagcgat aggcacaaaa gtagcgctaa gttgctatag cttagggatg gatgtcttgg
    19021 gttattctat ccgcgatgcc cagttggatt atgttcggca agcagattta gaaacagttc
    19081 tttctacctc ggattacatc gtagtcatgc tgccgttgac agaagataca aaaggattga
    19141 tcgatcaggc gaatatagaa aaaatgaaaa aagatgctgt acttatcaat gtaggaagaa
    19201 gtgaaattgt tgataaatat gctgttatgc aagcattgga aaaacaacat ctggctaaat
    19261 acttgactga ttttccagaa gaagagttct tagaaaatga ccgtatactt atgttgcctc
    19321 atctagggga tcgactcagg aagctttggc agacagcgga cgtttggcag ttgaagcctt
    19381 aaaagattat cttttgttcg gtacggttag agaagcggtc aattatccat ccgcacgaat
    19441 gtttttccaa gcgccgtttc gattcactat attttatcaa aagaaaacca atatattagc
    19501 agagatattt tctctgctga atgagaacga gctagcgatc gcagatatat cgcgtaacca
    19561 taagaatgag catgtctaca tattgattga catagacagt actgattttg aacagctcaa
    19621 tcaagtgaaa caacagctag aaaaaatttc tggagtacga aaggtccgct tattaaagaa
    19681 accagaaagg ccataaagaa agagaatatt tggatttcat taattcttct ttccgttctt
    19741 tactttcgaa tttcgtacaa ctaaaataag agaagcaaca ttcttttatt gatagaaatg
    19801 aaggagcaag catttgaaaa gatatataag cagactttcg accaaacaga aaaagatttt
    19861 aaaaggaaca gcagcaatca tcagtatctt ttttcttgtt gtttttagta atttattttt
    19921 gcagtggtgt caaaatgatc tatccgtaga ccttgcgttg aaatttgctt tttcttggca
    19981 tacggaaaaa ttcttcttag cctgtctcgt cttgctgatt attcttattt ttctgattgc
    20041 actagcagga tctgtccctt taggttccct tacgtatgtc gtagccatcg gtgtattagg
    20101 ttttgcaaat tatatgaaaa tgagttatcg gcaagaaccg atttatccag acgatttgaa
    20161 gatgatcact gaaatcggat tgctgaaaga tatgaccggc actatgctgt ttacagtgat
    20221 cttagctgcc gcaggaaccg tgataggatt gttttgctgg tacatgtttc gaagcctgaa
    20281 aaaaggaagg cgattccaat tgatccgatt aacgacactg cttgtggcaa ttggactgct
    20341 agggtatatc agtaatttca acaatcccga taatttgttg cgaaaagcat ataataaaac
    20401 ggcactatgg ataccttaca gccagaaaat gaactactat aacactggtt ttatcggtgg
    20461 gtttttgtac aatctgaagg tagaaccaat ggatgaacct gaaggatatt cgaaagaaaa
    20521 gatcaaagaa atcactgaaa aataccagaa gctagccgat gaaaaaaata aaactgtaga
    20581 ggaagagtcg ccgaacatcg tgttcgtgat gagtgaaagt ttctcagatc ctagtcgatt
    20641 aaacggcgta gaagtatctg gtgagccatt ggcagattat tatgaagtag ctgatcaaac
    20701 atatagtggc aatatgcttt ctcaaaacta tggcggtgga acagcaaata tcgaatttga
    20761 agcattgact ggtttttcta tggcgttatt taatgcgcaa ctcaccaccc cttacacgat
    20821 gcttgtacca aaaatggatc agcttccttc tatcgtttca gcgttaaacg cacaatcata
    20881 ccagacgaca gcgatccatc catacaatac ttcgatgtat aaaagagagg atgtttatca
    20941 gacattagga tttgaccagt tcatcagcga gcgtacgatg acctatacgg atacgataga
    21001 gaataatcca tatatttctg atgagtcagc ttacaaagaa gtaatgacac ttctcaagga
    21061 agaaaaaaca cctcagttca ttcatttagt cacgatgcag acacatatgc cctacgatgg
    21121 aaagtatgat aatctagact atcctgcaaa aacttcagat ggaagtggga cacttgactt
    21181 agagaattat ctgcaagata tttcctatag cagcacggca ctgaagcagt tcacggaaga
    21241 attgaagaat ctttctagaa ggactttagt tgttttttgg ggggatcatc ttccagggat
    21301 ctattcagat acgatccaag caaaaaatga caaacaaaca ttgcatgaaa cacaatttct
    21361 tatgttcgac agtaaaggag aattagaaaa gcaaacgact cacgatgcaa tcaccagtcc
    21421 gttttatttt gcagcgaatc taatggaaca gacgaatcag acaacaaatg gcttttatca
    21481 gctgctttta tcattagaac aggaacttcc ggcgtttgaa cgagaactgt actatcaaaa
    21541 cggacagtgg tataaagaag cgcaattcaa tcgctcgcaa caagagattt acgacgaata
    21601 tcaattgatc caatatgaca ttgtcgcagg gaaacaatat agtttagcag atggtttctt
    21661 tgagcacgaa taagaatggc gagctgtata gaaaccatta aattttttca agtacttgtc
    21721 atttgaggat aaaaacaagt agaataaata ataacaacaa tggagaaagt aggaaaacga
    21781 acatgttacc gaatattggc tattatgtgc agtccatcga tcaaatcgtc aaggaaacag
    21841 aagcaatcgg agaaggaatg catccgaaat atgaagtgat cagaaacgca attgatgaag
    21901 gaaaaacaag tgaactggac aaatcgactc ttgaagagat catcctgtta tttgaagaag
    21961 gaacaaaaaa atatcgggca atgcttatga aagtcagtac tttgcgtcca ccagcaaaag
    22021 caatgggcat tcataaaaat tttgaaaaag cttatctttc atatgtagca ggctgtgaag
    22081 aaatgatcca aagtatcgat cttgaatcag gggtagatga agatctattc aatacatctg
    22141 aagcaaaaca agaccaagcg acagaccaat tgacagctgc catccaaaaa atgactaatt
    22201 tgttgttgaa aaaataaaag aaatctttga taacaataaa aactagagtt atctaatata
    22261 aatgtaatat tacctttatg atagagtgat caaaatcctt tatgataatg acatacctat
    22321 aaccaacaac aaccttgtac ttactcaaaa tcgagtaaat actccttttc cgtcgagtct
    22381 aaccatctcg gcggtttttt atttgtagaa aagaacatca ggatagacgg aaaagtcttg
    22441 tcctgatgtt cttttcagat agtatggaaa gctgattagt tttctacatt ctgtacatat
    22501 tgttcaaagt agttctgtaa atcggctttg agtttctgat attcctcttc gctatactct
    22561 ttatcttcga ttttcccatc aaagaaagga atatttagtt cttcccgtag tctgtctaat
    22621 acgtcttgct gattcatttc attctccttt tcttactaac gatcaaaata aacacagaca
    22681 cattctggta catagacatc ttttttcagt aattccagta atcctgtttc aggatgacgg
    22741 cgatataatg tcagattatc ggtgttctga ttggcacaca cgacaaaatt attgcttgga
    22801 tctagtgcga aatcgcgggg aaaatcccct tctgttgaga tgatttgctt gttttgaatg
    22861 tgggaaccat cttcaaaaac tgcgaataca gcaatcgagt tatgtcctcg gttagaagca
    22921 taaaggaact tgccatcatt tgaaatgcgg atagctgccc ctccattttc accgtcgaat
    22981 tcttctggca aggtcgatat tttctgtaat tcttcaaaag cacctgtttc tttattataa
    23041 gaaagcacgc ggactgtgct gtctaattca ccaaataaat aagcgaattg tccatttgga
    23101 tgaaagacaa gatggcgagg gcctgttcct ggctctgcag tgaaggtagt tacaagattc
    23161 aatttgccgt tttcggacac atcataagta tacacacgat ctgtaccaag atcacatacg
    23221 actaatcgtt ggtcaggagt cagatccgta tagtggacat gcgcatgatc ttggttttta
    23281 tgaggacctg tcgcttcttg atggaatatt gaatctgctg ctttcagttc gccatctcct
    23341 aatatatggt agacatttac ttcaccttta tgatagttcg ccccgtatag taattgacgt
    23401 gcttcatcaa ccgctacata acacaaagga gcgccagact ctgttaccgc attcaaaaaa
    23461 tgaaaatcct tatcgtacgc accggcacca cctaatccat ccacactagt aactgtgtaa
    23521 agattgccag ctttactttt tgctacatag gtagggctat tttcttcaac agctaatgaa
    23581 aggttggcta attcttcttt ttctgtatca aggtcgatcg tatagattcc tttactttcg
    23641 cgacgcgtat aggttcctaa aattatcttt tcgatcatat gagctgattc ctcctaattg
    23701 taatgaaata gttttcgttt ttattctatc acataattat tccacacggc taaagaatga
    23761 gaatgtgtta tactgttttt gaaagcaatt aaaggagaga gtgcaatgat tcaggcaaag
    23821 attacagaaa ttgggacaga agcaataaac aaggaagaac ccatgctgat tttgttcgat
    23881 aaaacagcaa cagctgcctt acgaaaatat tcggtgattc aagaagtatc aaaagacgaa
    23941 cctttttctt tgaaagcagg cggaacaatt gcttttgatg atcaggtata tacgatcgat
    24001 tatgtcggtc cgatggccaa tgggaattta aagacagtag ctcacgtatc attgattttt
    24061 gatgcttgtc cagaagaagg acaaattgca aatggaatct atcttcatcc gcatagactg
    24121 cctgctattg gtttgggaag ccaaattttt tataactaac aggaggaatt atggaaggaa
    24181 aaaataagaa gaacagaccg gtatcgtggt tttggcgctg gtttttaaat aatcaagtag
    24241 tcacagcctt acttgttgta ttgttaatat tgctgatcgt ctttttattt acaaaagttt
    24301 catatttgtt tgcaccaatc tggcaatttt tagcaatcgt aggtctgcct atcattttag
    24361 caggaatctt gtattacttg atgaatccgg tagttgatta ctttgaaaag cgaaaagtac
    24421 caaggcttta cagtatcatt gggctgtttg ttttggttgt tgcactaatc gcatggggga
    24481 gcgtagtgat catccctaag attcaggaac aaacagtgag cttcatcagt aacttccctc
    24541 aatatgtaga tacgattgat aataaattaa ccgagatttt gcgggacccg ctgttcaacc
    24601 aatttcgcga acagttggaa acagcaggtg ataagttcat gaattcggca ggcgatatga
    24661 tccaagatat atctaagagt acagttcaga gtcttggaag ttttgtcggc gcggtagcga
    24721 ctattttagt ggctctgctg acgatgccgt ttatcttatt ttatctattg aaagacggta
    24781 aacaattagc accttatttt gtcaaatttt taccgacacg catgcaaaaa ccgacgttga
    24841 atgtgctgag tgaaatgaat agtcaagttt cttcttatat aagaggacaa ctaactgtgg
    24901 cttttgctgt ggcaatcatg tttatgattg gttttgcggt cattggttta gagtatgctg
    24961 tcacgttggg gattattgcg ggttttttaa acctgatccc ttacttagga tcatttttag
    25021 cgatgattcc ggctatcttt ttagccattg tagcaggtcc attcatgatc atcaaagtac
    25081 tgatcgtttt tgcagtagag cagacaatag aaggacgctt gatctctcca ttagtactag
    25141 ggaatcagtt agcaatccat ccagtaacga ttttattagt tctgctgact tctgggaaat
    25201 tgttcggaat catcggtgtg atcttgggga ttcctgttta tgcagcagct aaagtagtga
    25261 ttactcatgt ttttgaatgg tacacaacaa tctcttctct ttatgaagaa aatccgttga
    25321 aaaagcagaa agaataacaa aaagagacag atgggaaaat atccaaggca caaatgctgc
    25381 tttgaagatt ttttcctctg tctcttttgt atgcatttat ctcaattttt aaaatgattt
    25441 acctaaagca gcagctttat ctaatgcatt ttgcatcaac tcatctgcac ggtcagggtc
    25501 ataatcgatt ccttcgatga atagctgatc tacttgatcg atgcctacaa agtttaaaat
    25561 accttttaca tattgagagg caaagtcttg tccgttgtat acaccgccat tagattggat
    25621 gtgcagtgct tttttacctg tcactaatcc ttctggcccg ttttctgtat agcggaacgt
    25681 ttttccagct acattgacgg tatcgaacca agcttttaat tttgttggga tatttagatt
    25741 ccataaggcg tttgcgataa cgattttatc agcagcaagg aactgatcag tcaattcatt
    25801 gaaacgagca attttttgtt gttgactttc tgataaggaa gtgaattctg caccagcacg
    25861 taatgcttcc caacctgata ataattcttc atcgatttcc ggaatatcat cgcgataaag
    25921 ttccaaaaca gtcacttcat catctggatg attttcttta taacttgtta aaaaagacgt
    25981 caatgcctta actgtgcgcg attcttctgc agttagggga tgacctttga ctactaaaac
    26041 agatgccata tgcttacctt ctccttttat tgctatgatt gttataacta tgactaacaa
    26101 aatcagttta ctcgaaaaag aaaaggctat caagtcatat tgataaggtt gaagaataaa
    26161 taactaaaaa aagacagggt ataaaagagg ctgtgacacg attttttgtc acagcctctt
    26221 ttgaatctaa caagcaatat tcaagcaaca ctcgactgaa gagcttccat cccaccaatg
    26281 atataaacag cacgaaaacc attagcagta agttgatcgg ctaatgcttc tgatcgcctg
    26341 cctgtatagc ttagaatata ataaagtttc tttggatcta gattttttaa ttgtcttggc
    26401 aagatagcag cggggataga aagcgagtgg cttaagtggt tttttgtgta atctaaggta
    26461 tttcttgtat ctaaaacgat gctgtcagca tcgagaggca atttattgaa gtcttggaca
    26521 ctaattgatt gggccattta tctcatcctt tgtttagtat gtggctattt taatgggcaa
    26581 ctctcattaa ttcaaatgga aggattcctc ttatcacttt tttaatgtat cggatgataa
    26641 tgaaaaaaac tatgcaaatg tcttgcaaaa caggtaggag gtgttataat gacatagcgt
    26701 catatgacaa tgtgtcatct tgaaagaaag gagaatccat atgccgacac agacattttt
    26761 ccatttgcct aaagaaaaac aaaaaagact aatcgaggct gcgagaatcg agttttctag
    26821 agtaccctta aaagaagctt cgattgctaa tatcgtcaaa ttagcggata tccctagagg
    26881 cagtttctat cagtattttg aagataaaga ggatctttat ttttattact ttgagacaat
    26941 gagacgtgac agctctaaag atatgttaca actaatgaaa gaatccgatg gtgatctatt
    27001 caaaggattc gaatcctatt tttctaaaat gatttatgag attttggaag gcgagaatgc
    27061 ttctttttat cgtaacttgt ttatgaacat ggattaccgt gcctcaagaa aagtcgctcc
    27121 tcatacgagt cgccaaaaaa cgcctcacga tccacataaa gaacacaaac aagatgcaaa
    27181 agagttgctt gaattgatcg atcggacgaa gctgaaagta aaaaatgatc atgaattgga
    27241 attactgatc caattagtga tgaatactgt atttacaaca attgcgcatt cgtatcgctt
    27301 attttcaaca tctgaagatt actcagtaga gcaagcaatc agtgaattca atttgaaact
    27361 ttcttggctg aaaaacggag tatataaata gaaagaaggg aactagatgc taaaaatcgt
    27421 aaaacggatc tccttgactt cagcaattgc agctgcagtt ttcatggtga tccaagtact
    27481 ggcagattta tatttgccga cacttacctc caatattatt gataaagggg tagcgcaagg
    27541 agatgtcgat tatatttggc atacaggttt tgtgatgatc ggcttttctt tgatcagtat
    27601 cttagctgct atcgcaaata cattttttgc tactcgtgaa tcacagaaac tgggtaagca
    27661 attgcgtacg gatgtctata aaaaatcaga gagtctgaca aaagatgcgt ttgataaata
    27721 tggaacggcc tctttgatca caagaacgac caatgatgtg actcaaatcc aaatggttac
    27781 acaaatgttt ttacgtatga tgatcaatgc accaatcacg ttgattggcg cgagcatact
    27841 tgcttatcaa aaagatcatc agttgacaaa aatcttctta gttgtcatcc cagtaatgat
    27901 tattttgatc ggcggaatca tgtactttgc tgttccatta ttcaaaagta tgcaaaagaa
    27961 aactgaccgt ttaaacctgg tatttcgaga aggattgaca ggggtcagag tgattcgagc
    28021 attcgataag acacgctttg aagaaaatcg ttttgattta gcgaataaag attatacaaa
    28081 tacggcaatc aaagttaata cgatcgtcgc attgatgatg ccaatgatga cattgatcat
    28141 gagcggtaca aacgtagcaa tcacttggtt tggtggacac tacatagcag atatgacttt
    28201 ggaagtcggt aatctgattg catttatgac ttatgcgatg cagatcttga tcagctttat
    28261 gatgctttcc gctattttta tcatggtgcc tcgtgcacaa gcttcagctg accgtattaa
    28321 tgaagtgttg gatgaaaaaa tcggtatcca tgaccctgaa aatcctaaaa cagtttcttt
    28381 tgctggaaaa aacgcaacat tggccttcaa tcatgtcaat tatcgatatc atggagcaga
    28441 aaaacttgca ttggaagata ttgatttcca agccaagtcg ggcgagatcg tcgcaatcat
    28501 aggggggact ggttcaggta aaacgacctt agtcaatttg atccctcgat tatatgacat
    28561 cgaatctggt tcgatccaaa taaatggaac ggacattagt gacatgactc aatataattt
    28621 gcgagaatta atgggctttg ttcctcaaaa agcagttctg ttctctggaa cgatccgaga
    28681 caatatgcag tatggtaagc cggatgcaac agatgaaatg atttggaaag cattagaaat
    28741 cgcacaagct aaagattttg tttcagagat ggaagatggc ttagatagcc atgtggaaca
    28801 aggaggagga aacttctctg gtggacaaag acagcgtctg gcaattgcac gggcattagt
    28861 caaaacagca gatatctatg tgtttgatga ttccttctca gcgttggatt tcaaaacgga
    28921 tgccaatctt cgccaagcat tgaaaacgaa tatgaaagaa agtatcaccg ttttagttgc
    28981 tcaacgagta agtacagtaa tggatgcaga tatgatcctt gtgctagatg aaggaaaact
    29041 ggttggcaaa ggaacacatg aagaactatt agcaacaaac gaaacttatc aagaaatcgt
    29101 tcattcacaa ctaagagagg aggacttagc atgagtaaaa caaacacacg tccctctcat
    29161 ggaccagcag gcggtccagg acatggtgga cctggcagaa atctcggtgt aaaaggtgaa
    29221 aagcctaaaa atttctgggg aacggtacgc cgattgtttg gctatatgtc taaacgtatg
    29281 gttgcgatca tcgctgtatt tgtactagca atagcagccg tgatcttcca aatccaaaca
    29341 ccaaaggtct tggggaaagc aaccacagaa atattcaaag gtgtaatggc tggcgctcaa
    29401 cagatgcaac aaggccaaca agtaacaaaa tttccgatag actttgataa aatcgcgcag
    29461 atcattgcga cagtaatcgt gatgtatttg atttctgctg tattcaactt tttacagcaa
    29521 tttattatga cacgtgtttc ccaacgaaca gtttatgaat tacgtcatga tttggaagcc
    29581 aaaatgaatc gtgtaccgat ttcctattat gatacacata caaatgggga tatcatgtct
    29641 cgagcaatca atgatatgga taacatcgca agcacgctgc agcaaaattt gactcaattt
    29701 gtaactagtg cagtcacatt gattggggtc atcgtgatga tgctttcaat cagctggcaa
    29761 ttgacgttag tcgcgttatt gatgatccct ttaagcttga tcatcgtgat gatcgtagcg
    29821 ccgaaatcac aagtgttttt cgcagatcag caaaaaagtt taggtttatt gaataaccaa
    29881 gtagaagaaa catatggtgg tcataccatt gttaaaacat tcaatcatac tgaaaaagac
    29941 caggaagtat ttgaaaaaga aaatcagaat ctgtatgccg ctggttggaa agctcaattt
    30001 atttcggcaa tcatcatgcc gttgatgaat ttcgttaaaa atctaggcta tgtgttcgtc
    30061 gctgtactag gcggagtaaa agttgcaaat gggaacatga cattagggga tgttcaagcg
    30121 ttcttgcaat atacaacaca gttctctcaa ccaatcactc aacttgcaag tttgatgaac
    30181 actatccaat caacagttgc ttcagccgaa cgggtatttg aagtgttgga tgaagaagag
    30241 atgtccaatg aaaaatctgg ccttccagaa gaaaaagata caccatacaa agttcgtttt
    30301 gaaaatgttc aattcggcta cacaccagat aacttgctaa tgactgattt taacttggat
    30361 gtcaaacctg gtgagatggt cgctattgta ggtccgactg gtgcagggaa gacgacactg
    30421 atcaaccttc ttgaacggtt ctacgatgtt tctggaggta gtattcgtta cgatggtgta
    30481 gatacccgtg atctttcacg tgatgaattg cgcgcacagt tttcaatggt actgcaagat
    30541 acttggctgt ttacaggaag tatctatgac aatatcaaat atgggaacga cgatgcaaca
    30601 gacgaacaaa tcattgaggc tgctaaagct gctcacgtag acgattttgt tcgtaaattg
    30661 ccagaaggat ataacactgt attaaacgag gatgcaagca atatttcgca agggcagcgt
    30721 cagctaatca caattgcgcg ggcattctta gcaaatccag atgtcctgat ccttgatgaa
    30781 gcaacatcta gtgtagatac acgaacagaa atactgattc aaaaagcgat gaaccgccta
    30841 ttggaaaacc ggacaagttt cgttgttgct caccgcttgt ctacaatacg cgatgcagac
    30901 aacattattg tgatgaatca tggttcgatc gtagaaacag gaaaccacga tacattgatg
    30961 gaaaaagacg gattctatgc agatctttat aacagtcagt tttctgaaga agaagcttca
    31021 tagaatagta caaatttatt atttttttta ggggctgtga caaaagtcgt gtcacagtcc
    31081 ctttttccga ataaacggtg ttcaatcagc tgtccttcgg tattagctca aaaaacggca
    31141 aaatatgaga agctattttt ccgttttgta tctctaatac tcaggtcaaa ccgctgattt
    31201 tacaacctct tccttaaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    31261 caacaaacct gagaagctgt tttttgaaat tgctccttgt gactcggagt taaacggcag
    31321 gctcacaacc tcttggtaaa cggtgttcaa aagccagttt ctcggtatta agccaaattt
    31381 atgcaaaaat gtagaaagcc attttcacaa aatttttctt actactcgaa actaagcgac
    31441 tttttcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    31501 accaaacgaa aaataggagg agctattttt gtttggctgt tcttattact tgatgcagaa
    31561 cagctttttc acaacctctt tttataagag tttttactca ttaatttaca aaaaacaagc
    31621 aaaatgcgaa ttttctcaca ttttttttaa caaaatcaaa gttattgctt gaaagaagaa
    31681 gaatccatcg ttaaaataaa gcttgacgta gaggggagca agaaatatga aagattttca
    31741 attgggggct gccacctatt taaaaagtgt agccattcgg gtgaaagacc gagataaaat
    31801 gattgatttt tacaagaacg ttgttggctt tgatttgaaa agagaagaaa acgaattagc
    31861 cattttagga acagaagaag ctggaagtga aatgttgctg ctggaagaaa gtcctagagc
    31921 gaatgatcac atgggggtga tcaaaaagct gaaccgtttt tcattgatta tccctagcac
    31981 agaagaaatg gcagatatcc tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc
    32041 tttagaagat catggacgta tggggatttt gctcgtagac ccagaagaga atgaattgga
    32101 aatttaccat gaagagaata agaacgacca aagagaaatt ccagaaacaa tggatcaaga
    32161 agcattatta agaaaatctg aagaaaaatt cagtaaacta tcaacaggtt cttattttga
    32221 aaaaatccat ttaaatgtta ctaacttaac aaaagaacgc caatttctta atcatgtact
    32281 tggtttgaag gtacataatg aagaagaggg catccatgta ttgaatgaag gagatttcca
    32341 tgttggtttg acagaagcta gcggaggcgc aattgatttg cctacagatg aagtgattgg
    32401 acttgatttt ctgaaattca tgatcgatac tgaaagcatg gaaggactga ttgctcactt
    32461 gcagcaacta tcagttgatt tcttcttaga caaaaagcac acagtactca caatctatga
    32521 tgctatagga atcgaatggt ggttcgtcag tgagccaaaa gaaaagaata aaactgacat
    32581 tcgatgcgac gcttaacttt ttctttctta acgattgtcg catgtctcgt cattttatga
    32641 acggtcatac tatcgcttat tctagagata gaaagagaaa atagtatacc gggggaaact
    32701 gcgcggtttt tcgcgcagtt tttttgtgta aaaaaggttg attcctcata gattaagctt
    32761 taagcttgga ctgtaagaat ctgatagaaa agcaggagaa aaagatgtat ccacacacga
    32821 aacagcttat tttgaaaaag atggaagaag gattttatcc aggtgtagca gtcgccttca
    32881 taaaagaaaa tcaagtagaa acatttacag ccggctttgc ccaagtcgtt cctgaaaagc
    32941 agctgatgac agaagatctg ttgtttgatg cagcctcact gacgaaagtc gttgcaacca
    33001 cttcattagt tttacgtcta ttagaagatg gaaagataga actcgatcaa ccgcttcata
    33061 aatatcttcc tgaatttgaa aatcagacaa tcactttgcg tcatttactg acacatactt
    33121 ctgatatcaa aacatggata ccaaacagag ataagctatc tcaggaagaa ttgatcgccg
    33181 cttatttgaa gctagaacca ggtgagcaac taggaaaaga agtaaaatat acagatgccg
    33241 gtacgattct tcttgggttg atgctagagg aaatttacca agaaactgca gtggaaatct
    33301 ttcaagaaca agtattggaa ccattaggaa tgatgaacag cttatttctt ccttttcctt
    33361 ccaaaaaaat cgttcccaca gaacaactgc cttcgggtga ggttttgcgt ggacaaactc
    33421 atgatccgaa agcaagagtt ttggctgaac atgcaggtaa cgcgggtctc tttattgatt
    33481 tgaaagacag cattactttt gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc
    33541 gttttttaga agaagagacg attgattctt tactgcaaga tcaaacaccg aaaaaaaatg
    33601 gctatcgttc tttaggatgg gacttaaaaa aggatgtaga agacggtcat gcgattcttt
    33661 ttcatactgg ttatacaggt acgtttttct tgattgatat aaaaacacaa gaagcgttga
    33721 tatttttgtc aaatcgcgta catcctgtcg atcatcgtgc ggagtatctc atatgtcgag
    33781 atgaaattgt tgaaaacttt ttgaaagaga aagcggtatt accgaaataa tgctataata
    33841 gctatgaaaa atgagaggag tttgaattat gcaggaacct atgtttttaa aaccagtttt
    33901 tcaagaaaaa atttggggag gaagtcgttt acgttcagtc tttggttttg atatccctaa
    33961 cgataaaatc ggagaagatt gggcgatcag tgcgcatcct catggtgtca gtgtagtaga
    34021 aaatggcgaa ttcaaaggga aaagattaga tgaactatgg tcagaacaca aggaattgtt
    34081 tggccatcct tcagaaccag tgtttccatt attgatcaag atcttagacg cagaagatga
    34141 tctatctgta caagtacatc cagatgatgc ctatgggatg aaacatgaag gtgaattagg
    34201 aaaaactgaa tgctggtata tcatcgatgc tgaacctggt gcagaaatca tctatggtca
    34261 tcatgcaaag acaagagaag aactagctga gatgatcaaa gatgggcgct gggatgacct
    34321 tttgaagaaa gtaccagtta aaaaaggtga tttcttttat gtaccaagcg gaacgatcca
    34381 tgctattggt aaaggaatca tgattttaga aacacagcaa agttctgaca cgacttaccg
    34441 cgtgtatgac tacgacagaa aagatgcgca aggacaaaca agagagctgc atatccaaca
    34501 atcgatcgat gtaacaactg tccctgcgaa aacaccagaa ttacaaatca aagaagtccg
    34561 aaaagggaat tcatcgatcg tcacttattt agaaactgag tttttcaatg tatatgagtg
    34621 ggatattaaa ggaatcacat ctttcaaaaa acaagcccct tatacattga tgacggtaat
    34681 cgaaggagca ggagaccttg tcgttgacgg aaaaacatat ccattagaaa aaggaacaag
    34741 ctgtattatt ccaaatggcg tttctgagtg ggcggtccaa ggtgaactag caatcattgc
    34801 gtctgaacca ggcaaatagt tcccagatag ctgaaagcag cagacggttt ttcgtttgct
    34861 gctttttcta cataaaatcc gtccatcaat aaatagaagt ctggtgaaaa agatggaata
    34921 caaatggaaa gcaaacaaca gcagtcgcgt gtctatgaaa caactgagaa aagctggaca
    34981 tcacttaaat gaaatgatca tgccaaatct aagtctgttt atcgcttggg gaatcttgtc
    35041 attagcaaca aaaggtgtgt ctggcgattt gcacaccaca ttagaagagg tatgccattg
    35101 gatgatccag ctgcttttac ccatcttgat cagttattca ggcgggaaat cggtaggggg
    35161 acaaaaaggg gcagtcgccg gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc
    35221 tccacaaatc gtaggagcga tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt
    35281 tgtttctaga ttccatgtga aattcaaggc tggatatgaa atgcttgtca gcaacctact
    35341 agtaggactg actggtagcg ttatttgtat aattggaatc gtttttattg aaccagtttt
    35401 aaatagtgtt cacttgttgc tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt
    35461 accacttgtc agtctgatct tagaacctct aaaagtacta tttttcaata atgcaatcaa
    35521 tcatggtgta ttgacacctt taggaatcga attctcacaa acagtcgggt actcttcctt
    35581 atttttgatg gaggcaaatc ctggacctgg tttagggatc ttactttcta tcctttgttt
    35641 tgcagaaaaa caggaaaaag taaatgcgag tggagcattg atgatccatg cgattggcgg
    35701 gattcacgaa atctattttc catttgtctt gctgcgaccg tatctatttt tagctgtaat
    35761 ggctggtggg gcaagcggca cgttgatatt tcaatggtta gatacgggat tagctgcacc
    35821 tgtatcgcct gggtcactac tcgtgatttt tgccaatgcg ccttatccac aactagctgg
    35881 ggtaagtata gggatagcaa tcagtacatt tgtgaccttc atttgtgcag cgatactatt
    35941 gaaattacca aataaagaaa aagagaacaa aacggtcaaa gaaaggggag aaatcttgga
    36001 accatctatc attcattccg tcatttttgc ttgcgactca ggtatgggat caagtgctat
    36061 gggagccgcc ctgctgaaaa agaaattagc agcagaaaaa atcgatcttt ctgtcggata
    36121 cagctctatc tatgaattga ataaccaagt aaatcagctg gttatcgtgc aacatgaact
    36181 agcgggtctt gcaagagaaa aagtacctca aagtcaaatc tatgtactgg atcacttttt
    36241 ggaaacagat atcttgctgt cacgaataaa agaagcgatc caatttccaa agcgggaaga
    36301 aaaaaaagag atagcagata tcacagaaca aaaaacgtcg cctgctaata ttgtattttt
    36361 gtataaaaac aacagacgcg gctcacaaac tatgggcatg acgattttaa gtcaattagc
    36421 aaaaaaagcg aaaaaacagt ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca
    36481 agaagaaacg atttatatcg cacaaagaga actagctgta actgaacagc tagaagaaaa
    36541 gatccctcgg ctagttctag ttgaacattg ggtagctacg aatgaatacg aaaaactcgt
    36601 gaagggagaa atataagatg tatttatcgc ccagagaaaa acaattatta gcagaatttc
    36661 tcagtagccc gtcacctgta tctatccaaa aaatgatgaa tttattgaag gtcagtaaac
    36721 ggactattta ccgcgaatta gataatcttg aagtctcatt gaaatcggta aatgcttctt
    36781 tgaaaaaagc agctagaggc tcatttgtca ttgaggcatc agaagaagta ttagagcaat
    36841 taaaagaaaa tacagatggg aaaagtaatg acttatccac tgctaaacga caacacgcga
    36901 ttttagccga gttgttactg acgacagagc cgttgagtct tgctcatttc atggaatcat
    36961 atcgcatcag taataccact ttttatgcag atatcaaaca gttggaagaa agtattcgcc
    37021 aactgccttt ggaaatcatt cggaaccagg gatacgagat cgaaggaccg gaaaaatacc
    37081 gccggcttct gacagcgaat gtattagaat tagaaatcaa tgaatatgaa ctttttcata
    37141 gtatttcttt cgatgcatca ctgaattatt tttttcgatt cgttgatccg caacatctat
    37201 ctcttgcacg caaagtaatc ggggaagaat tgatccaaaa aaagaccaat ctaagtgata
    37261 gaaagcttga acatttggtt ttgatgctga cattgacgat ggagcgtgtg acgaaaaatc
    37321 atttactgac aaatgagaca tataatagtc tggcaaacaa agaattacta cagacagcta
    37381 aacgattgtt ttcacggatc gcttctgaga caaaacaact ttatccagta aatgagatcg
    37441 tcttttttgc aggattatta aatgacttca cgaactcatt tgatcagaat ttctttgaag
    37501 agacgttcga tacacagctt gcttatttag taaaacaact gattcaagaa gtaagtgaag
    37561 agacgaatgt ccatttttat gaagatgaaa ctttgtacaa aatgctgttg actcatttat
    37621 ctggtgtgtt ttcaagagcc gttttacaag aagagacact gaccaatccg attttagaaa
    37681 aaatcatggt tcaatacgag gaagttgcga atgcaatccg taaagcagta gagcggattt
    37741 ttcaagaaaa aaaattatcc gaagaagaga tcgcttatat ggtccttcat tttgcgaatt
    37801 cattggaacg aagtccgaaa gaaagcaata tcagtattgc aggattttcg ccaagtggat
    37861 tagcttctac tagcatgttg gaactacgat tgaaaaggta ttttcccttc atccaacaaa
    37921 ttcatttcta tcggattgct gatttaaaaa aaatcgattt acaagaagaa tatgatgttg
    37981 ttatttctac atctatttta ccaggatacg gtggaaaata tattttagtc tccccgctac
    38041 tgctagaaga tgaagtcaag cagctaaaag aagctttttc gcatattgag aagaaaaaaa
    38101 ggcaaaaaat tacgtataac agcaataacg agtggatgat agaagatact tatgaggaaa
    38161 caatgcattt catggatcaa atcaatcaat tactcggtcg tttttttatc caagaactgg
    38221 ataatcctga gacgatttcc gaaacgattc gcagcttatt tccttatttt cctgaaggac
    38281 tagtaacgga tgaagaggtg gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta
    38341 tcggtattcc tcatacaaat atggggttat tccatacatc cagctcattg atttctaaac
    38401 cgatcttttg tattttcaat ttgaaacatc cgttgaccat tgaagggatg gatagacagc
    38461 cagtcgattt gtcccgtatg ttagttatgc tggcgccttc gccaatccaa gaaaaagacg
    38521 ggattctttt aggaaagatc agtggagcaa tcatcatgaa tgacttgaac accgagatct
    38581 tccgctcagg aaataaagaa atcgtttatc agttgttagc gaaaatcctg attgaagaaa
    38641 taaagaaatg aaggtgaaaa gatgatacag ctaccagcag aaaaaatatt gctcgatcaa
    38701 tcctatcaaa attggaaaga agcgttgacc gagatcggcc agtggatgac ccaacaaggg
    38761 ggaatcaaac cggcatatat ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat
    38821 atcgggaatt tcgtagcctt gccacatggg actgacaaag atgaagaagt agtagaagaa
    38881 ggaatctatt tagtccaagt gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa
    38941 atcgcgacac tcttatttgt agtggcttta aaaaaagaac atcagttagc ggtgctccaa
    39001 gatttagcct ttttctgttc agacatcgac caagtaatgg cgctcagtga tgcggaaacc
    39061 attgaagaag tccaaatgat ttttgaacat ggacatatat aaccaatgtt cacagtaaaa
    39121 aaagctgcct tgcatcaagt aataacttca accaagataa aaataagagg agctattttt
    39181 gttgggaggt tcttattact gcggatgcaa gcttttgaaa actatttatt ctagaaaacc
    39241 gtttatttga aataggggac ggtgacaagg tttttgtcac cgcccctatt atttttgtgt
    39301 tttaatagct cttttttata tagatagtgc ccatacctat gaataataaa acgatactcc
    39361 atataccaag tgtcaagata ccattcatga aagtaacctg ttctgaagct agtggcgaga
    39421 aaatatctcc tcctatgatc acattttgaa aatcaacata agaagttggc aagtaatgac
    39481 tgattgattc tatccatcca atcgaagaaa acaagcgata ctctgctaag aaaagacaaa
    39541 gaagtgtacc taaaagattg actagagaat gttcaaataa agcagataga agaaaactga
    39601 ttgtacttaa aaagagcacc cacaacaaca ggaacagaaa gctctggaaa agatactcat
    39661 acatcggcag gattcgtatc tcctgaccaa tgatcgttcc tacaggataa cggaagtctc
    39721 ccaaaccatt tttgattatt actagtatag agacgatcaa aaatggcatc acgagactta
    39781 gcatagctgc accataataa atggtaaaac ggatgctatg tttctttacc atactatccg
    39841 gtaatagatt gactagagag attacctgct ttcttttttc ataagagatg atggaagaaa
    39901 gaaacaaggc ggttatacat aaaatcaaaa aactagaaat cattcctgaa aaaatatttt
    39961 cgtagtaatt tgctaaagag agatgcgctg gcaagtcaaa aatagaatat ctttggatct
    40021 gatgttcttt catataacgg agcaactcga cacgtttttg ttgttcaatg attggaattc
    40081 cttgtctttg accagaaatc agtcgatcca gtatatcttt ttcataatga taagttgctt
    40141 cgaccatttg ctggacattc cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt
    40201 tgcttttctc taaactttcc acaaattctt tctcattctc atcttctttc aacgtttcaa
    40261 tcaaacgttg catctcttca tagttatgat tcagttcatc gaattctgta taagcaagtt
    40321 tatgatcaaa aaaaagtggg aatgcaaaga gcacgaccaa caatagagga aataaaaagt
    40381 ttactttgtt ttttagtaag agattgcgtt caaatgtaag taaagacata gttacacctc
    40441 atataattta cgataaatag ctaaaatctc ttccatattt gaggattctg tgacgatttc
    40501 tccatttttt agaaaacatg ctttagaaca aatcgattgg atattcgcga tagcgtggga
    40561 agaaagaaga atcgtcttcc cctctttctg ccattgtctg atgaggctat tcaagatatc
    40621 aatacttcct gggtctaatc cgttcaacgg ttcatcgaat aacatgacag atgaatcact
    40681 gatcaagtaa gcagcaagca agacatgttg tttcattcct agagacattt ttccaatcgg
    40741 caaagtaagg tactcagtca tcttcaatcg ttggaaaaca gtttggcatt ctactttgct
    40801 ctgccaatag tatttgatca aagtcaaata atcaacagcc gatagatagt cggataactg
    40861 attcgttgat tctagaaaaa agaaagaccg gcgataatct gtataagcag taggagagat
    40921 gttgtgaagg gacaattgat cataagttgt tggcagcagt tgggcaatgc cattcagcaa
    40981 agttgttttg ccaaatccgt tcggggcaat caaagcagct acttctcctg attctactaa
    41041 aagattcaat tgtcgaatca cacgtttttt accacgtttc atgttcatgt ttttgatcag
    41101 taatgtcata gataggcctc caatataaaa tttaataaca taatagaatg gaaaacatct
    41161 aaattaaatg ctaacaaaag ttattgtttg catattataa aaaaattaaa ataacagttt
    41221 tatttaaaat tgaagttttc tgataccgaa atctttttat tttgctgaca tactcaaacc
    41281 tttggcacaa agtttcgtgc caaagtacga ctatttctct ttttagtaag cggatacaat
    41341 tagagtatca aaaggagaga gggagagaca aaatggaacg gacgattccc aaaaacaaca
    41401 tttcgctaaa agcgagggta caaaaattag gcagtacttt atcaagcatg gtcatgccaa
    41461 atattggagc actgattgcc tggggtgtat taacggctct atttatccca gatggttatt
    41521 taccaaatga ggctttcgct accatggtaa gcccaatgct aacttatctg attcctttat
    41581 tgatcggtta tactggcgga aaagtaattg ctggtgaccg cggatcagtt gttggggcaa
    41641 ttgcaacgat gggtgtgatt gtcggaacag atattccaat gatgctaggc gcgatgatca
    41701 tgggacctct tggtggatat gccatcaaga aatttgatca gctattccaa aaacggatca
    41761 aatccggttt tgaaatgcta gtcaataatt tttcagcagg tcttatcggt tttggattag
    41821 cgttattagg tttttctgcg attggtccag ttgtcgatgc attgactcaa gcaatggcaa
    41881 aaggtgtaga gatcatttta tctgcccatt tgattccatt gacaagtatt tttattgaac
    41941 cagcaaaaat cttatttttg aataatgcaa tcaatcatgg gattttgaca ccattgggaa
    42001 ctgaacaagt agcagaggct ggaaaatcta ttctattctt gcttgaagca aatcctggtc
    42061 caggacttgg cgtattactt gcttttactt tgtttggcaa aggagcagca aaatcttcag
    42121 caccaggagc catgatcatt cactttttag gcgggatcca tgaaatctat tttccttacg
    42181 ttatgatgaa accgttatta ttcttatcag ttataggcgg aggaatgtca ggaagtttca
    42241 ttttccaatt gatggatgct ggactaagag cgccagcttc tccagggtcg atcattgcga
    42301 ttctagcgat gacaccgttc aattcttacc ttcctgttat tttgggcgtg attgttgccg
    42361 cagctgtttc gtttggtatt tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg
    42421 aagacttcga aaaatctgtt caagagacaa aggcagctaa gcaagcttcc aaaggtgccc
    42481 aaggtattcc tcaaggagct acattctctg gcgtacaaaa aatcattttt gcttgtgatg
    42541 caggaatggg atcaagtgcg atgggcgctt cgatcttgag aaaaaaagta caggaagcag
    42601 acttgcctca aacggtgacc aatcaagcaa tcagccaact gacagatgac tcgaatacat
    42661 tgatcgttac tcaggtcgaa ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt
    42721 ttgtctcagt agaaaacttt ttgaattcac ctcgatatga tgaaatcatt caagaattaa
    42781 gcagcaagca gaaaacatca gtttctgaaa ttgagaatga gccagcagaa gagtttcttt
    42841 cagcaaacat caaacaaatc aaagagattc tacttgtcca cgatgatcgt tccggatcag
    42901 caacaatggg gatgaccgtg ctcaaagata tattagacaa gaaccattta actatcccgt
    42961 taagaaaagt caatatccaa gatttaaaag atgaacctga ttcactgatc gtagccaaag
    43021 aaagtttagc ggaaaaagta cagatggctg cacctcaatc cattcattta tcagtcaaca
    43081 gcatggtcaa tccgcaaaaa tacgaatcga ttgcacaata tttgaaacag acagcttaag
    43141 ggaggaaaac agcatgcaaa gtttgccatt cgacatgatc gagctcaatc gatcttttaa
    43201 aaccaaagaa gaagcgatcc gctattgcgg tcagatgctt gtagaagcag ggtgcgttga
    43261 tgaggaatat atcgatgcca tggtagatag agacaaaatg ttatctgttt atatggggaa
    43321 ttttatcgcg attcctcatg gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat
    43381 ctgtgtcgtt caagtaccag atggtgtaga ttttggtacc gaagaagaag aaaagattgc
    43441 gactgttttg ttcggtattg caggagtcgg agatgagcat ttgcaacttg tacagcaaat
    43501 cgcactttat tgcagtgata tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga
    43561 agaagtaacg gaaaatctag caattgctta aggagagaga aaaatgagag cagtacattt
    43621 tggtgcagga aatatcggtc gaggatttat tggagaggtt ttggcagcta atggatttga
    43681 gattactttc gtagatgtga atcacacgat cattgatgct ttaaatgaaa gaaaagaata
    43741 cgaaatcgaa ttagcagatg aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat
    43801 caacaatcaa acaaatccag aaaaagtaat ccaagaaatc gctgaagcag atttagttac
    43861 taccgccatt ggcccgaata tcttaccttt tattgctgag tttattgcaa aaggtatcca
    43921 agaaagagaa aaggaaggaa gcacaacatc tttagatatc attgcctgtg aaaatatgat
    43981 tggcggttca gcatttctag aaaaagaagt gtataaatat cttccggaaa catcatttac
    44041 tgacaaatat atcggctttc caaatgcagc agtcgatcga atcgtcccat tacaacacca
    44101 tgaagatcct ttattcgtcc aagtggaacc tttcaaagaa tgggtcattg acgatagcca
    44161 aagaaaaaat aaaaaaatcc aattaaaagg ggttttatat gtagatgatc tagaaccata
    44221 tatcgaacgc aaactattta gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc
    44281 cttgttaggt taccagacca ttgatgaagc aatgcaagat gcgttagttg tggcacaatt
    44341 aaaatcagtt ttgcaggaaa caggaagttt gctgattgcc aaatgggggt ttgatgcaga
    44401 gcagcatcat gcgtatattg aaaaaatcat tcatcgcttc caaaacaaaa atatctctga
    44461 tgcgatcaca cgtgtcgctc ggacaccatt acgtaagtta ggttatcaag aaagatttac
    44521 acgtccagtg agagaactac aagaacataa cttgacttgc ccacatttga cggcaacaat
    44581 ggggattatt tttaattact atgatcctga agatgaacaa agccgtcaat tgcatgaaat
    44641 gaagatccat gaaaacttgg aacaattgat tcaagaagtc acaggaatca atgatccgaa
    44701 aacaatcggg aatatcaagc aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag
    44761 gatacgaaaa aggggaatgt gacgaaattt ttgtcacatt cccctttttc gaataactag
    44821 gctgtttgtc cttcaagata gggcagatcc aattctttac cgtaacgttg ttctaaagct
    44881 aggcggatca aacgagctgc caggttctca tttcttgcaa ggataggtcc atggaaataa
    44941 gaaccgaaga catttcggta aatgacacct tcagattggt cttcaccatt atttccttga
    45001 ccttgtacca ctttccccaa aggtcgttca ccttcaccta agaaagtccg accattgtgg
    45061 ttttcaaatc cataataagt ttcattgaat tcttcgttat ggataacgat atcaccgata
    45121 aaccggttat tttcctgact taacgtgtag tgatccagtg cactgattcc ttggatcttt
    45181 tctccattag ctcccatgta gtagtgacct agcaattggt agccaccaca aatggctaac
    45241 atgacgccat cattttcgat atagttgatc aaagaatctt tctttttctg gatgtctttt
    45301 gaaatgatta attgctcaaa gtcttgacca ccgccaaaga aaaccaagtc atatttatca
    45361 gcatcaaaag gttcatgtat actaacgatc tccgtatggc aagaaacccc cattttttct
    45421 gctatatatt tcaacatcaa aaggtttccg ttatcaccat atgtgttcat caaatttcca
    45481 taaaggtggg cgatattgag ttcataatta gccacggttc attcctcctt tgataaatcc
    45541 ttgagcagct agttctttac gaagctgcaa tacagcagtg taagtagcta aaatatagac
    45601 atgctcagtt ggcaattctt tgattttttc gattacttct ggtaattctt ttgtttctgt
    45661 caatttattt tctggaatcc cggcaacttt tagacgtaaa gtcatatctg tatgacggtc
    45721 tcctccagaa ataacagctg ggatatccat atcggcaaag ttttcaaaat taccatccca
    45781 aatccagctg acatcgattc cgtctgcgta attggcattt aataaagcca ctaaagaaaa
    45841 tggataagga gctagaccaa tcatatcgat tacttgattg atacctacag gatttttgac
    45901 aagtactaat gtacattttt tcccattgat attgatgact tcttgtcggc cgaatacttt
    45961 ctcgtcatat cctaaacctt gtttgatttt atcaggatcg acattgaagt actctgccac
    46021 tgcagtagct gctaaagcat tgtagacatt atacataccg ccaacttcga ttccgtattc
    46081 atgaccatcg atcacgaaat ctgctgacac attgtccatc cgtaccatat cagttaatcg
    46141 ataatccaat tcaggacgtt tgaagtcaca atttggacag taatatttcc ctaagtttgc
    46201 ataagtgatc attttataat gcaagatgtg atgacaattt ggacataaga caccgtctgt
    46261 attgtaatga gccatttgtt cacgatcttc ttcatggtca aagccataat attttctagg
    46321 attgactgtt tcgatagaat tgaagattgg tgaatcaccg ttactgataa tcgttgcatt
    46381 tggtgacttt gcggcaccgt ctacaatcat tttataggtc gtatagattt ctccataccg
    46441 atccatttga tcacggaaaa tatttgtaaa taagaacagt tctggtttca tatattctgt
    46501 gactttgctc aaacttgctt catctatttc taggacagca aactttttct gtcctttttt
    46561 tggttttgcc tgcaagaaag tcgatacaat tccctgtacc atattcgcac cagtagggtt
    46621 ggttagaact tcatcgaatt gctggcgcaa aatattgact gttaaagcgg tagtcaatgt
    46681 tttaccgttt gttccggtga caacgacgac ttggtagtct tttgccaaac tatctagtat
    46741 atgtggatcg actttcagcg ctagtttacc tggtaaactt gatccgcctt taaaaaaagt
    46801 ttttaatgcc cattgcgacg tttttccaac tgcaatcgca aaatgactgc gtattcccat
    46861 tatgtaacct ccaaaaacga tttgctgacg aataaattca actaatcata ccatatgttg
    46921 aaaaaaaggt taaattgctt ttcttattct taagaaaagg ttacggctga aagtgcatga
    46981 aatcggacaa aatcgcatga tttaacaaaa aaacagcttg aaacaaaatg attgtttcaa
    47041 gctgctgtga ttttattatt ccctcttacc tatattccca ttttaaccga taataatttt
    47101 ctcgcttgga tattcatagc ctagatctcg tgtttctttt ggcacgaata acatcaatgt
    47161 atatagcatt ccgatccgcc caataaacat cagtgtagca atcgtgattt tgcccacaac
    47221 agacaagtct ccggtaatcc ctaaagataa accagttgtt ccaaacgcag aagttacttc
    47281 gatgatgatc gagatcagcg tttgttcttc tgtagcagat aaaaaaacga tacaaaagaa
    47341 acacatacca agagaaagca taaagacaac gactgatttt cgcacatcat cttgatcgat
    47401 tctccgtcca aaaatattga tgttatcttc acttttgagg aaggaatata gatacaaacc
    47461 gatgattgct acagtagttg ttcgaatccc gccgcctaca gaactaggac tgcatccgat
    47521 aaacatcagt aaagaaaaaa tgatcagtgt tgtgatttga aaatcaccca aatcgtgaat
    47581 ctgcagacca gcattacgag tcgtaatcga ataaaacatc gaattgatcc atttgacact
    47641 catattggaa tcttggaata aatggtcctt ttccaataga taaatcaaga cagttcccga
    47701 aacaaacaag ataacaaaag ctaacacagc taatttggta aacaaggaaa aacgaaacgg
    47761 aagcttcgca ttggaacgtt tgtataatag ccattcccgg cactccatca atacaggaaa
    47821 accaattcct ccgacaaaaa tcaaaaacat gatcaggatc aagaaaaaat agtcatgtgc
    47881 aaagggcttg atggaatccc cagtgacgtc aaacccagag ttcgtgacag ccgatatcgc
    47941 ctgataaaaa ccgtaaaaaa cggcatcacg ccaacgatca aaatagcctc tgtaataaaa
    48001 ataaatagaa aagaatgtcc caaaaagtaa ttgaaaccag attaaaatag caaaagtgat
    48061 ccgaatcagt cggacgatac cagacaaacg cggttgattc atatccgtca tgatcagctg
    48121 tcgctgtttc agtgtgatcc gtcgtttaga aaaaatgaca aaagcagtag aaatcatcat
    48181 gatccccaaa ccaccaactt gaaataatat ttccagcagt ataataccgt tatcattgaa
    48241 cacagaattg atatcaaaag tcgatagtcc agtcacactc actgtactga ttgccatgaa
    48301 cagcatgtcc acaaaagaaa catggctatc tggttcgcga aaaatcggca tataaaatag
    48361 aacgagtgat aagaccgtca tcaatatata ataaaaaacg atgatttgga tcgatgagaa
    48421 atgattggca gcaaacttct gtcctcgccg ctgcagcctt cgaaagaata aatcaacatg
    48481 cacagtacta tcctcctgac aatagtcgtt tctaccagta tacaagggga attaggaata
    48541 ctcaaagaaa ttatcaaaca gataacaata cagctttcga ttaaaataga aatggcttat
    48601 aataaggaaa atcctagaaa aaagaaggag gaggaataac aaattgatta atgctcaaca
    48661 aatcgtagaa aaaatgaaaa atcaaaaaat aaattatgat acagttctga aaaaaatgat
    48721 catgcaatgg gagaaagaag aagtaaagcc taagatatta atacatacat gctgtgctcc
    48781 ttgcagtacg cacacattgg aattcatgtc tcaatatgca gagatcacgt tatttttttc
    48841 aaattcgaat attcatccta aaagtgagta tttgcgaaga ttacatgaac aaaaacgatt
    48901 tgtagaccaa tttaatgaga aaactgacca tcatgttgcg ctaatcgttg atgaataccg
    48961 tccaaatgaa ttcaccaaaa tggttttaac aaaccatctt gaaaacgaaa aagaaggtgg
    49021 tgctcgctgc agtgcatgct tcaatatgcg attagatctt gttgcgcaaa aagcgcaaga
    49081 actaggatac gattactttg gaagtgccct gaccatctcg ccaaagaaaa atgcagcttt
    49141 gatcaatcag atcggaatgg atatccaaaa gatttacggg acacaatact tgccaagtga
    49201 tttcaagaaa aacaaaggct atgaacgctc tttgcaaatg tgcaaagagt atgatattta
    49261 tcgccaatgt tattgtggtt gcgtgttcgc tgccaagcag caaggatgcg atctgaaaga
    49321 aatcaatcgg gaagcaaaag cttacttaaa aacatcaacc gtagtttttg aggaaaatta
    49381 aagaaatctt tcggaaaaac gtaagaaaaa aataaagaat ttatggtaga aataaggtag
    49441 actgtataat ttgtccgtag taaagtaagg aaacaagaat aaatttcaat gaaggaagag
    49501 ataagatgcg aaaagtagca cctattttga agccaatcgc tgtattaaca atctcgacga
    49561 ttgtcgcgac tactacaggt attgtgaata gttccagcta ccgtgcacca agttcaagtt
    49621 ctagtactgt agaagaaaca acaccaacta cttcagtctc gacatctgaa ccaacaacaa
    49681 cggaaagttt gaatgaaacc actacgacaa gtacatggga cgatacaaca actagttcgt
    49741 cgtatgatga gacatataca actgacagct caagcgaaac aagtaatacg gtaccagatg
    49801 gcactccatc ggattcaacc aatccaagta caagtccgga tacaagtgct agctccgaaa
    49861 cgataccaag cagtacgact gtagatgata acacgcaaaa tgatgatgca gcacgtacag
    49921 cacagcaaaa ccaagattca ggaaataccc aaacgtataa ccagcaagca ggtgattctc
    49981 aatgacattt ttactagacc ttatcaacgg ggtgcataaa ttccttggct atttagacat
    50041 cagtcctaaa tatcttaacc gtggatatac gattttaagt gtgattccca cgctttattt
    50101 attgagaatt gtttatggac tttggcaaaa ccaaaactat cttcaatttt tcttatatgg
    50161 acttgcattt ctagtgcttg tttattttac tgttttaaat gtattttatt actttttaga
    50221 taaaaacacg aaagccgatg tcactcagct ttttgtgaag tatttacctg atgaagcgtt
    50281 caacatccag acagaagaat cgatagataa tggaaattcg atcgataaag tgaatacaca
    50341 agaggtcatg gtttcttatg acgaagatta tcaattgaaa ttagccgaga atatgcggta
    50401 tttgattgga aatggtgaga tcaaaacaaa tgaactcggt cgcatggatg gatttctggt
    50461 cgatagaaat acattgtatc cttattacta tgtcaagaaa acaggagaga aagagtatgt
    50521 tctaaatatt ggacgaagct actcggacat ggaaaaagta gggaccattc gacttgactc
    50581 aacagaagac acattggaac ctgtcggatt gttcatcgtc ggcggtgatt ttgtcaaaga
    50641 cgggttacgc tatcatgagc cttatcgtct gaaattgatt gcaaaaaagg cacagtcgca
    50701 ggaagaacca gtggtttata gtcgtagcca aagaagaaga cattcataat gtgatgaaac
    50761 gccgtgtatc agtggttgcg ataaaaacgt ttattcggaa taagggggat gtgacacgtc
    50821 ttttgtcacg ttcctttttt atataacgag aaagataaga cgaacacatc acttattctt
    50881 tcctttcatt tataaacatt gtagaataaa gaaagtacaa cgaagtattg tgaaaaaacg
    50941 taataaataa cgaggaaaat acaagtagga ggtaagctgt gaaagaattt ttgaaaaatt
    51001 gggggatttt gatccttgta ttagcggcta ttttattagc tagagtgtac gtgtttacac
    51061 cagttactgt aaacgggcat tcgatggacc ctacattaag tgatggacaa cgattgattt
    51121 catctaagat ctcaaactat gaacgaatgg atattattac tacaaaggaa ccgggggatg
    51181 aagaacggat gattgttaaa cggatcatcg gtatgcctgg agatacagtc aagatggaaa
    51241 atgaccagtt gaccattaat ggcaaaaagt atgatgaacc ttatctggat gagttcaaaa
    51301 aagaattttc agaagataag ttacaaggag aatatgctta tagctcagga ttccaagcac
    51361 aagcagaaag ttcatccacc tttacgagtg actttgaata tactgtgcca aaaggaaagt
    51421 atcttgttct aggcgataac cgcttgattt caaaagacag ccgaatgttt gggttagtcg
    51481 acaaagatat gatccaagga aaagttgttt tccgttattg gcctctttca gaaattaaaa
    51541 tcatgtgatg aaaaaataaa aagaagctgt gacatgtttt ttgtcacagc ttctttttcc
    51601 gaaaattcct tcttatttct tggagttgct cacttttttt gcaatctcat tttaagcttt
    51661 ttggctgatg gataagaaaa gcgaagcgtt tttgttcata aatagctttt ccccacgatg
    51721 gaaggtatga ctagtagcag agacaaagcc cgttttttcc aatcgattat tcaattcttc
    51781 ttggctaaag ccattatgga ctttcggatg actaatttgt tcattcttgt caaagtctac
    51841 gatgatcaat tgaccgccag gagctaaaat ttcataaagt tttgtaagaa tattctccgt
    51901 atcaggaatg tgtaaaagta caagtgatag aaggatgaga ttagcttgga cagcaggaaa
    51961 ttcgacagaa gcatcagcgt ggatcgttcg gacatttttc agatctgctg cttggatctt
    52021 ttcttctgcc attttcagca tagtttctga agcatcggca atgatcagtt ctttgaatct
    52081 ttccgcaagc ggtaaactaa caagcccagt tccgcctccg taatccagca ggactttttg
    52141 gtggctgtcg cgcggtagat aagtagttag ttcttcgcga ataatcttgg ctaatgcttg
    52201 acggtcaggg gaatcataat ggttagcgat ggaatcaaaa atattttttg tcatcaaata
    52261 gctccttacg taaaactttg aaattttaac ttattatagc acgtgaaaag aaagaaagca
    52321 tgctatactc aattcaaaaa gggagaatct agaaagaata tgccgatatc gtttgtaaaa
    52381 gatagagagg aaaaaggaaa gtgtgtccga gaaatattac tagacttgcc tgagtggttt
    52441 gggcttcctg aatcaacgga aaaatatatc gaggaatcaa gtaaactacc tttatggtgt
    52501 gaaaaaagaa aagaagaata tttaggcttt atcactcttt cacagacaag tgaagatact
    52561 gccgaaatct attctattgt atgggaataa aaaaagacta tcatcaccaa ggattaggaa
    52621 caaaactttt taaagaagca gaaggttacg cagcaaaaca ttacaaatat ttgcaagtga
    52681 agactgtaga cgaggggcac tactctatat atgatcaaac tatttgtttc tacgaatcat
    52741 taggattttc aaggttggaa gtttttccaa atttatggga tgaatggaac ccatgtttag
    52801 ttcttgtcaa aaaattggaa caaaagtgag caatagattt aatactaatc tgtgcttgat
    52861 gaaaattctc actgtaaaaa atgctgaaaa tcaagtataa taccatagga acaaactgga
    52921 aggtgaaaaa gagcatggca caactatttt ttaagtatgg cgcaatgaat agcggcaaga
    52981 cgatcgagat cctaaaagtc gcacataatt atgaagaaca aaataagcca gttgtcttaa
    53041 tgacaagcgg tattgataca agagatggtt taggcgttgt ttcaagccga atcggactga
    53101 aacgtaatgc gatgccgata tttgaagaaa cagacgtatt tgacgtaatt cagaaaatgg
    53161 aaagcaaacc ctattgtgtg ctgatcgatg aagcgcaatt cttaaaaaag aaacatgtca
    53221 ttgcttttac gagaattgtg gacgaattgg atattccggt tatggcattt ggtttgaaaa
    53281 atgattttcg aaacgagtta tttgaaggat caaaatattt attgctttat gcagacaaaa
    53341 tcgaagaaat gaaaacaatc tgttggttct gccacaaaaa agcaatcatg aatttacatt
    53401 atattgatgg aaaccctgtt tatgaaggcg atcaagtgca aattggcgga aatgaagctt
    53461 attaccccgt ttgtcgaaaa cattactttc acccatcaat gattacagaa ggagaataag
    53521 catgtacgat caattacaag caattgaaga ccgttatgaa gagcttggag aacttttaag
    53581 tgacccagaa gtgatttctg atacaaagcg ttttatgcag ctttcaaaag aagaagcaaa
    53641 cacgcgcgaa acagtcgaag tttatcgtcg ttataaagaa gtagtacaag gaatcaccga
    53701 tacagaagaa ctgcttggtg aaaaactgga tgatgaaatg gctgaactag caaaagaaga
    53761 attatctgaa ttaaaaaaag aaaaagaagt cttagaagaa aaaatcaaga tcttgctttt
    53821 accaaaagat ccaaatgacg ataaaaatat catcatggaa atccgtggag cagcaggcgg
    53881 agacgaagca gcattattcg ctggcgatct tttcaatatg taccaaaagt atgctgaagc
    53941 acaaggctgg aaaacagaag tgatggaagc taacgttacc ggaatcggtg gatataaaga
    54001 agtgatcatg atgatcaccg gagacaacgt atattcgaaa ttaaaatatg aaagtggtgc
    54061 gcaccgcgtc cagcgtgttc cttcaactga atcgcaaggg cgtatccata cttccaccgc
    54121 aacagttgtc gtgatgccag aagctgaaga agtagagatc gatattgaag acaaagatat
    54181 ccgtacagat atctatcatg cttcaggtgc tggcggacag cacgtcaata aaacagcatc
    54241 tgctgtacgt ttgactcact taccaacagg gatcgttgtt gctatgcaag atgaacgttc
    54301 tcaaatcaag aaccgtgaaa aagcaatgaa aatcttgcgt gctcgtgtgt acgataagat
    54361 ccagcaagaa gcacaaagcg agtatgatgc caatcgaaaa tcagcagtcg gaactgggga
    54421 tcgttcagaa cggatccgga cgtataattt cccgcaaaat cgtgtgactg atcatcgtat
    54481 cggattaact atccaaaaat tagatcagat tttagcaggg aaattagatg agatcgttga
    54541 tgcattgatc atgtatgatc aaacatcaaa actggaagct atgcaaaatg ggtaaaatga
    54601 cctatcgaga agtcctgtca cgggcttctt cttttttaga ggatcatggg aaagaagcat
    54661 atagtatcca atttttattt ttggaacgaa aacaatggaa aaaacttgat tggctccttc
    54721 atatgaacga agagatttca gaagaagaac agcggttgat cgaaaccgat ctgcgacttc
    54781 tgcttgcaga tcatccacct cagtatttgc taggatatgc agatttttat gatcatcggt
    54841 taaaggtaac tgaagcaaca ctgatccctc gaccagaaac agaggagtta gtcgaatggt
    54901 gtttagacga gacgcctggt gtaccgttag aagtgataga tattgggaca gggactggag
    54961 caattgctat tagtttgaaa gcagcaagaa aaaattggca tatatctgct gtggatcttt
    55021 cagaagaagc attagaagta gcgaaagaaa atgctcagaa agaaggaacg aaaatcagtt
    55081 tttatcatgg agatacatta ggaccagtaa tggatcagca atttgatgtg attatttcta
    55141 atccgccgta catcagtcgg aatgaatggg aactgatgga tgaaagtgtc cggtcattcg
    55201 aacctaaaat ggcccttttt gctgagaacg atggattagc tatttatgaa aaaatagcag
    55261 ttgaagcacc aagcgtacta aaatcaaatg gaaagatttt tttagaaatc ggatttcgcc
    55321 aaggtgaagc agtaaaaaaa atatttcagc aagcatttcc agataaaaaa gtggcgataa
    55381 aaaaggatct ttttggcaat gaacgaatga tccaagttta tggttgaagt tgtggatgaa
    55441 aaatttttta tttgtttttt tattataaaa aaacacttat ttgacaagga tgtaaacgac
    55501 tttttggttt atcaacaagt ttatccacta ttgacaaata gattatccac aaacagtgga
    55561 aaacttgagg ataaactcag acaaaataag aatgtatgaa aaaggggttg tggataaact
    55621 gtggaaacga aacgatatga tgaaacagaa ttgcaagagg cagccgaagc actgaaagaa
    55681 ggagaacttg ttgcttttcc taccgaaaca gtatacggat tgggcgccaa cgctctttta
    55741 ccaaacgctg tcaagaaagt gttttctgta aaagggagac cgcaagataa tccattaatc
    55801 gtccatgtcg cctcttttga acaagtaaaa gagtatgtgg acaactttca tccggcagca
    55861 gagaagattg tgaagaactt ctggcctgga ccattaactt tgatatttaa gatcaaaaaa
    55921 gacactttgc cctcagttgt gacgggagga ttatccacag ctgcatttcg tataccggat
    55981 aacaaaaaaa cactagaagt aatcgaactt tccggtgttc cgcttgtcgg tccaagtgca
    56041 aatacgtcgg gaaaaccaag tccaacgact gcagatcatg tatatcatga tctacaagga
    56101 aaaattaccg ggattattga tgatggagct acgcgaattg gtgtagaatc cactgttctt
    56161 gatttaagtg atccaacagc catgcctatg attttacgac caggtgcagt aacgaaagaa
    56221 caaatcgaag cagtcatcga atctcctgtt gctattgatc agcatcttgt aaaagaaaat
    56281 gaaacaccaa aagcccctgg gatgaaatac aaacactact cacccgatac acgtgttctg
    56341 atggtcagag atggcgattg gtcaactgca gttcaatggg caaaaaataa gaagatccgt
    56401 gcaggtgtca tagcaagccc cgaaattgcg gatcaagtgc ggacagatac tgcagctgtt
    56461 tatatgtata acgacaactc agtagaagcg gcggctaaag gattgtttgc aggtctaaga
    56521 ggattagatg aaccaacact tggattagat ttgatttttg tacaagtgta tccagaaact
    56581 ggattgggga atgcttacat gaatcgcttg aaaaaagcag caggacaaaa ttattttgaa
    56641 aaatgatcaa ttggttattg tcgcattctt ttagtatgat acaatgctat caagtaaatc
    56701 ttctatcagt gaacgaaaaa ctaagcgtaa ctaatcgtaa tagaagagat acgaatattt
    56761 ggcaatttgt ttttgatggg aaaaataaaa ttgtaggaaa ataaaaagga gctgttgttt
    56821 gtggtagatt acaaaacgtt tgaccctgat ctttgggctg cgatcgccaa agaagaagaa
    56881 agacaagaac ataatttgga gctgattgcc tctgaaaact ttgtctccga agcagtgatg
    56941 gctgcacaag gaagtatctt gacaaataaa tacgcagaag gttatccagg gcatcgctat
    57001 tatggtgggt gtgaatttgt cgatatcgtt gaaaatttag cgattgaccg tgcaaaagaa
    57061 ttattcggag cgaaatttgc gaacgtccag cctcactccg gctctcaagc caacacggct
    57121 gcttatctcg ctttggtaga accaggggac acgatcttag gaatggatct ttccgcaggc
    57181 ggacatctaa cacatggatc accagtcaac ttcagtggca aaacctatca ctttgttgct
    57241 tacggtgttg atccgacaac agaagttatt gattataatg tggtacgtat tttagctaga
    57301 aaacatcaac caaaattgat cgtagcaggt gccagtgctt acggacgaac gatcgacttt
    57361 gcgaaattca gagaaatcgc agatgaagta ggcgcaaaac tgatggtaga tatggcacat
    57421 atcgcaggat tagtagcagc aggacttcat cctaatccag ttccttatgc agatatcaca
    57481 acgactacca ctcataaaac attgcgtggc cctcgaggcg gcatgatttt gacaaacgat
    57541 gaagcattag caaaaaaaat aaatagtgcg gtatttcctg gtattcaagg cggccctcta
    57601 gagcatgtga ttgcgggtaa agcagtagca ttcaaagaag cattagatcc tgcatttaaa
    57661 gaatatagtg aacaaatcat cgccaatgca aaagcaatgg tcaaagtttt caatcaagca
    57721 attggaacca gagtcatttc aggggcgaca gataatcatc tgatgttgat cgatgttcgc
    57781 gagttaggta taaatggaaa agaagcagag agcattttgg acagtgtgaa tatcactgta
    57841 aataaaaact cgattccttt tgaaacacta agtccattca aaacaagcgg tattcgaatc
    57901 ggcacgccag caatcactac tcgcgggttc aaagaagaag atgctgtaaa agtggctgaa
    57961 ctagttgtca aagcattaca agcaaaagat gataacgctc aactagacga agtcaaaaca
    58021 ggtgtacgcg aattaactga aaaatttccg ttgcataaaa aataaaaatt taaattccat
    58081 tagtaaacag aaaagttcaa tgttgggctt ttctgtttat tttttgggaa aacggattct
    58141 ttgacaatat cggtgatgct ctctagtata tgaggtgtaa ttttagtctt cacactaaat
    58201 ctcagggagg aaaagtcatg actgatcgta tctcgttaga aaaggaagca gtagcattca
    58261 gtgatgcaaa tgcgcctcat ccgcgtatct atgaactagc accggaagaa ggaagagttt
    58321 tactagaaga agtacaagaa tcacctgtga acaaatattc cgtcgatatt gaagacttga
    58381 tggtggatac tggcgaatgg ggaaaactca atattcgtct cgtacgacca gaagggaata
    58441 tggataaatt accagttatt ttttatattc atggagcagg ctgggttttt ggcagtgctc
    58501 aaacccatga taaattgatc agagaacttg cggttcgtac gaattccgtc gttgtctttc
    58561 cagaatatag tcgatcacca gaagcaaaat atccaacagc aattgaacaa aattatgctg
    58621 tgttgcaaca gctgaataaa ttagccaagt caaaaaatct ggatctcgag aatctaatcg
    58681 ttgcaggaga ttcagtaggt ggtaacatgg ctacagtcat gacgatctta acaaaagaac
    58741 gaaagggcct cccaatatcc aagcaattgc tgtattatcc agtcacaaac gctgattttg
    58801 atacagattc ctatcatgaa ttcggagaaa actatttttt gacaagagaa ggaatgatgt
    58861 ggttttggga ccaatatacg acgaatgaag aagagcgtgc tgaaatcaca gcttcgcctt
    58921 tgagagcaaa tataaatgaa ctaaatgatc ttccttcagc aatgatttta acgggagaag
    58981 cggatgtatt aagagatgaa ggtgaggctt atgcgcgcaa acttcgtgat gcaggggttc
    59041 ctgttacaca agtacgcttc caaggaatca tccatgattt tgtcatggta aatgctatgg
    59101 atcagacaaa ggcaacgcgt gcagcaatgg cattatctac ttcttggatc aagaaaaaag
    59161 aataagcttt gcttgtccgc aggaaaactt cttttcaaaa acggattttc ttaggaaaaa
    59221 tggcgagttt gactagtcaa tcacttgatt ttgttttaca attaaaaaga aacgacagaa
    59281 caaaggagag cttaaaaaat gggcaaattt caagtgatcg atcatccatt gattcaacac
    59341 aaactaacta ttattcggga caaaaactgc ggaacaaagg tttttcgtga agtagtcgat
    59401 gaaatcgcta tgttgatggc atatgaagta tcacgggata tgccattgga agacatcgtg
    59461 atcgaaacac cgatcactga aacaacacaa aaaacgttgt ctggtaaaaa agtagcgatt
    59521 attccaatct tgcgcgcagg tattggaatg gttgacggta tcttggaatt gattccagct
    59581 gctaaagtgg gacatatcgg tctttatcga gatgaagaaa cattggaacc acatgaatat
    59641 ttcgtcaaac ttccagaaga cattgatgca cgtcagttgt ttgtagtaga tccaatgttg
    59701 gcaacaggcg gatcagcaat catggcaatc gatgcattga aagaacgtgg cgcgtcgaac
    59761 attaaatttg tttgtttggt agcagcacct gagggagtaa aagctttgca agaagcgcat
    59821 cctgatattg acatctatac agcttctcta gatgaaaaat tgaatgaaca tggttatatc
    59881 gttcctggac taggggatgc gggtgaccgt ttgtttggta caaaataagt aaatacgaaa
    59941 aagggtgtga cacaactttt gtcacatcct tttttggtca tttttaacgt ttgatgcaga
    60001 acagcttttt cacagctttc gtttgcatat aaaaaaacca tcccaaaaat gcagagatgg
    60061 tcaaattcaa atctttttta gttctttgta gttaacgatt actacaccct tactttgcgt
    60121 catcaagttc gcattttctg cagtttcatc aatctcaaca acagcggaat ttttcaattg
    60181 ctttgaaatc gttccagttt gcgacttgcc gtgaattgtg aacattacgt gagtattgat
    60241 cttgaatttt tcaccaattt ccgatggtac gatagttgta tcaaattttc caaaagttgc
    60301 cattttcata aaacctgcct ttcacatcaa ttataccacg ttttatgaaa aatatcatga
    60361 aattaaacta attttccatc ggactcttta caatcagcca aatagagtat ataatttctt
    60421 ttggataagg ccagcttagc tcagttggta gagcaacgca ctcgtaacgc gtaggtcgta
    60481 ggttcgatcc ctatagctgg cattaaaaga gaagacagat atatcaagga tttttaatcc
    60541 attgatacat ctgtcttttt gtttaaagga aataaagtat gctttcttag caatagagca
    60601 tgtattattt ctataaaaat ttaggagaat aaaacgacta gcaatatagc tgtgaaatca
    60661 aatatcacat gaactgccca acttgtaatg atcgagttgc ttttaatggc agcttggttg
    60721 aataacaatc ttgccacccc ttgtatcaaa aggatgtgtg ctacagtgtg taaaatattt
    60781 ccattatcat aagtactgta atggataaat ccaaagatga ttgctgacaa gaaactagcg
    60841 acaggaatgc tgaacttact agaaaaaata gcgagaaaat acactgagaa caattcttct
    60901 ccaagtatca tgataggcaa aataataaat aataacgggc tgaaagcctc atttattgga
    60961 ttgcctgtta aaccccattt caaaatttgt tgcaaaaata ccgagactgc ggcactgatt
    61021 actacattga taaaaaaata tttggctata tttttgacag gagcgacagg tttcgagaac
    61081 atttttttcg tatctttttt accaaaaaca agtgtaaatg ctataaatcc agtgatagtt
    61141 aaagaaaaaa tgctaataat ggatggcgca aatagcaacc ctaacaaacc gagtacaacg
    61201 acaatagtgc ccaatatccc ttttttaagt tctatttttt ttcctaaaat gttattcatg
    61261 ataataactc ctactat
//
LOCUS       DAZUMM010000006        56207 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000006 DAZUMM010000000
VERSION     DAZUMM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.006"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..486
                     /locus_tag="ACG8E6_000313"
     CDS             <1..486
                     /locus_tag="ACG8E6_000313"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS5060764.1"
                     /translation="SITLIIFLILILLVVLVPRIFGLTPYIIEDKEMSPYYAQGNLIY
                     VRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKR
                     NIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKF
                     L"
     gene            614..1297
                     /locus_tag="ACG8E6_000314"
     CDS             614..1297
                     /locus_tag="ACG8E6_000314"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFS5060765.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1606..1679
                     /locus_tag="ACG8E6_000315"
     tRNA            1606..1679
                     /locus_tag="ACG8E6_000315"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1640..1642,aa:Arg,seq:tct)
     gene            1838..2197
                     /locus_tag="ACG8E6_000316"
     CDS             1838..2197
                     /locus_tag="ACG8E6_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFS5060766.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2320..3951
                     /locus_tag="ACG8E6_000317"
     CDS             2320..3951
                     /locus_tag="ACG8E6_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFS5060767.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMLQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4013..4654)
                     /locus_tag="ACG8E6_000318"
     CDS             complement(4013..4654)
                     /locus_tag="ACG8E6_000318"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS5060768.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4840..4916
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4936..5244
                     /gene="rplU"
                     /locus_tag="ACG8E6_000319"
     CDS             4936..5244
                     /gene="rplU"
                     /locus_tag="ACG8E6_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFS5060769.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5271..5615
                     /locus_tag="ACG8E6_000320"
     CDS             5271..5615
                     /locus_tag="ACG8E6_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFS5060770.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5628..5921
                     /gene="rpmA"
                     /locus_tag="ACG8E6_000321"
     CDS             5628..5921
                     /gene="rpmA"
                     /locus_tag="ACG8E6_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFS5060771.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6355..9162
                     /locus_tag="ACG8E6_000322"
     CDS             6355..9162
                     /locus_tag="ACG8E6_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5060772.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9353..9844
                     /locus_tag="ACG8E6_000323"
     CDS             9353..9844
                     /locus_tag="ACG8E6_000323"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS5060773.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9947..10936
                     /locus_tag="ACG8E6_000324"
     CDS             9947..10936
                     /locus_tag="ACG8E6_000324"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS5060774.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            10969..11772
                     /locus_tag="ACG8E6_000325"
     CDS             10969..11772
                     /locus_tag="ACG8E6_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFS5060775.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11793..12707
                     /locus_tag="ACG8E6_000326"
     CDS             11793..12707
                     /locus_tag="ACG8E6_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5060776.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12790..13164
                     /locus_tag="ACG8E6_000327"
     CDS             12790..13164
                     /locus_tag="ACG8E6_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFS5060777.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13303..13548
                     /locus_tag="ACG8E6_000328"
     CDS             13303..13548
                     /locus_tag="ACG8E6_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5060778.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13720..14784
                     /locus_tag="ACG8E6_000329"
     CDS             13720..14784
                     /locus_tag="ACG8E6_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFS5060779.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15030..15455
                     /locus_tag="ACG8E6_000330"
     CDS             15030..15455
                     /locus_tag="ACG8E6_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5060780.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15448..15897
                     /gene="nusB"
                     /locus_tag="ACG8E6_000331"
     CDS             15448..15897
                     /gene="nusB"
                     /locus_tag="ACG8E6_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFS5060781.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            15994..16848
                     /locus_tag="ACG8E6_000332"
     CDS             15994..16848
                     /locus_tag="ACG8E6_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFS5060782.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16849..18186
                     /gene="xseA"
                     /locus_tag="ACG8E6_000333"
     CDS             16849..18186
                     /gene="xseA"
                     /locus_tag="ACG8E6_000333"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFS5060783.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18191..18415
                     /locus_tag="ACG8E6_000334"
     CDS             18191..18415
                     /locus_tag="ACG8E6_000334"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFS5060784.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18417..19307
                     /locus_tag="ACG8E6_000335"
     CDS             18417..19307
                     /locus_tag="ACG8E6_000335"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS5060785.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19316..20134
                     /locus_tag="ACG8E6_000336"
     CDS             19316..20134
                     /locus_tag="ACG8E6_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFS5060786.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20241..20690
                     /locus_tag="ACG8E6_000337"
     CDS             20241..20690
                     /locus_tag="ACG8E6_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS5060787.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20708..22390
                     /gene="recN"
                     /locus_tag="ACG8E6_000338"
     CDS             20708..22390
                     /gene="recN"
                     /locus_tag="ACG8E6_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFS5060788.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22445..22561)
                     /locus_tag="ACG8E6_000339"
     CDS             complement(22445..22561)
                     /locus_tag="ACG8E6_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFS5060789.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22634..23590)
                     /locus_tag="ACG8E6_000340"
     CDS             complement(22634..23590)
                     /locus_tag="ACG8E6_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS5060790.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23761..24129
                     /locus_tag="ACG8E6_000341"
     CDS             23761..24129
                     /locus_tag="ACG8E6_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFS5060791.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24299..24730
                     /gene="mraZ"
                     /locus_tag="ACG8E6_000342"
     CDS             24299..24730
                     /gene="mraZ"
                     /locus_tag="ACG8E6_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFS5060792.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24751..25710
                     /gene="rsmH"
                     /locus_tag="ACG8E6_000343"
     CDS             24751..25710
                     /gene="rsmH"
                     /locus_tag="ACG8E6_000343"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFS5060793.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25715..26116
                     /gene="ftsL"
                     /locus_tag="ACG8E6_000344"
     CDS             25715..26116
                     /gene="ftsL"
                     /locus_tag="ACG8E6_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFS5060794.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26117..28309
                     /locus_tag="ACG8E6_000345"
     CDS             26117..28309
                     /locus_tag="ACG8E6_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS5060795.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSTYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28335..29297
                     /gene="mraY"
                     /locus_tag="ACG8E6_000346"
     CDS             28335..29297
                     /gene="mraY"
                     /locus_tag="ACG8E6_000346"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFS5060796.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29311..30672
                     /gene="murD"
                     /locus_tag="ACG8E6_000347"
     CDS             29311..30672
                     /gene="murD"
                     /locus_tag="ACG8E6_000347"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFS5060797.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30698..31786
                     /gene="murG"
                     /locus_tag="ACG8E6_000348"
     CDS             30698..31786
                     /gene="murG"
                     /locus_tag="ACG8E6_000348"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFS5060798.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31801..33021
                     /locus_tag="ACG8E6_000349"
     CDS             31801..33021
                     /locus_tag="ACG8E6_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFS5060799.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33177..34502
                     /gene="ftsA"
                     /locus_tag="ACG8E6_000350"
     CDS             33177..34502
                     /gene="ftsA"
                     /locus_tag="ACG8E6_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFS5060800.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34525..35766
                     /gene="ftsZ"
                     /locus_tag="ACG8E6_000351"
     CDS             34525..35766
                     /gene="ftsZ"
                     /locus_tag="ACG8E6_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFS5060801.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35783..36460
                     /locus_tag="ACG8E6_000352"
     CDS             35783..36460
                     /locus_tag="ACG8E6_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFS5060802.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36474..37073
                     /locus_tag="ACG8E6_000353"
     CDS             36474..37073
                     /locus_tag="ACG8E6_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFS5060803.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37154..37375
                     /locus_tag="ACG8E6_000354"
     CDS             37154..37375
                     /locus_tag="ACG8E6_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFS5060804.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37424..38206
                     /locus_tag="ACG8E6_000355"
     CDS             37424..38206
                     /locus_tag="ACG8E6_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFS5060805.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38227..38934
                     /locus_tag="ACG8E6_000356"
     CDS             38227..38934
                     /locus_tag="ACG8E6_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFS5060806.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39196..41994
                     /gene="ileS"
                     /locus_tag="ACG8E6_000357"
     CDS             39196..41994
                     /gene="ileS"
                     /locus_tag="ACG8E6_000357"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFS5060807.1"
                     /translation="MKMKETLQLGKTKFSMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42043..43569)
                     /gene="zwf"
                     /locus_tag="ACG8E6_000358"
     CDS             complement(42043..43569)
                     /gene="zwf"
                     /locus_tag="ACG8E6_000358"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFS5060808.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43584..44231)
                     /locus_tag="ACG8E6_000359"
     CDS             complement(43584..44231)
                     /locus_tag="ACG8E6_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS5060809.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44415..44744)
                     /locus_tag="ACG8E6_000360"
     CDS             complement(44415..44744)
                     /locus_tag="ACG8E6_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFS5060810.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44921..45649
                     /gene="sfsA"
                     /locus_tag="ACG8E6_000361"
     CDS             44921..45649
                     /gene="sfsA"
                     /locus_tag="ACG8E6_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFS5060811.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45665..46678
                     /locus_tag="ACG8E6_000362"
     CDS             45665..46678
                     /locus_tag="ACG8E6_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5060812.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46678..47955
                     /locus_tag="ACG8E6_000363"
     CDS             46678..47955
                     /locus_tag="ACG8E6_000363"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS5060813.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48018..50720
                     /locus_tag="ACG8E6_000364"
     CDS             48018..50720
                     /locus_tag="ACG8E6_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5060814.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50872..51189
                     /locus_tag="ACG8E6_000365"
     CDS             50872..51189
                     /locus_tag="ACG8E6_000365"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5060815.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51219..51539
                     /locus_tag="ACG8E6_000366"
     CDS             51219..51539
                     /locus_tag="ACG8E6_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5060816.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51647..53107
                     /locus_tag="ACG8E6_000367"
     CDS             51647..53107
                     /locus_tag="ACG8E6_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5060817.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53175..53396
                     /locus_tag="ACG8E6_000368"
     CDS             53175..53396
                     /locus_tag="ACG8E6_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060818.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53427..53609
                     /locus_tag="ACG8E6_000369"
     CDS             53427..53609
                     /locus_tag="ACG8E6_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFS5060819.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53609..54022
                     /locus_tag="ACG8E6_000370"
     CDS             53609..54022
                     /locus_tag="ACG8E6_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFS5060820.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54145..55326
                     /locus_tag="ACG8E6_000371"
     CDS             54145..55326
                     /locus_tag="ACG8E6_000371"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5060821.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55397..55561)
                     /locus_tag="ACG8E6_000372"
     CDS             complement(55397..55561)
                     /locus_tag="ACG8E6_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060822.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55715..55788
                     /locus_tag="ACG8E6_000373"
     tRNA            55715..55788
                     /locus_tag="ACG8E6_000373"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55749..55751,aa:Arg,seq:cct)
     gene            complement(55857..>56207)
                     /locus_tag="ACG8E6_000374"
     CDS             complement(55857..>56207)
                     /locus_tag="ACG8E6_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5060823.1"
                     /translation="INQKELFENFEVKNPHNFVFFNLKNGLVSSNAVSKYLRKKLKEL
                     GIEKQFTLHGLRHTHASILLYQGVNILSVSKRLGHSSLETTMSTYLHIVRELEDQDKE
                     KINAVFDSLYKNDN"
BASE COUNT        18558 a         9494 c        12103 g        16052 t
ORIGIN      
        1 tctataacgt tgattatatt tttaattctt attcttctcg tcgtcttggt tccccgaatt
       61 tttggtttga ctccttatat tattgaggac aaagaaatga gtccatatta tgcacaagga
      121 aatctgatat atgtacgacc gaaaaataca aatgagattt tagtcggaga tattattaca
      181 tattatgaaa attcaggaaa aaaaatctcg acccgtcgcg taatagccgt tgaaaacaac
      241 cacgaagatt tttatacaaa agctgataca aagacttata ttgagcctgg agtagtaaaa
      301 aagagaaata ttatcggtag tcccatcttt cagattccat atattggact agtcctttca
      361 aaaacggcat ttgcatggat tcaggggtta ttctttacga ttgctttttg cctaacagga
      421 atcacagcat ggaatacctt tgtagagttg aaaaagaagc ggagacgaac acagaaattt
      481 ttgtaagaat gttaaaaaca taaatgtatt ccagagatcc aacgactctt ctgaacatga
      541 aacacgagaa aacactcatg cagcttgtag atttagtcag ttgttcgtat aatgtgtgaa
      601 gaggaggagt agtatgcgca atgaaatgat gcatcgtcct gtagtcaaag aagaactatt
      661 ggattttatg cgtacccagc aaaaaaaatt accgggtgag ctaggcaaac tggaagaaga
      721 agctcatgta gcagaagtac cagttattcc ccatgaaaca gttgtatttt tgaaattttt
      781 acttggacag cttcagcctg aacgaatctt ggagatcgga gcagctatcg gtttttcatc
      841 aagtgtcatg gccacgacaa tcggagaaaa cgctcatgtt acgacaattg atcgtttcga
      901 tgtcatgatc gagaaagcta aaaaaaacta tgaacgtcta ggactgacag ataaagtgac
      961 tttgcttgaa ggtcaagcag cagatatctt gccggagttg tctggtccgt atgactttat
     1021 ttttatggat agtgcaaaat caaaatatat tgaattttta cctgaatgct tacgtttgtt
     1081 aagaaaaggt ggcgttttga tggtagatga catcttccaa ggaggaacga tcttgctccc
     1141 agatgaagag attccccgtg gcaaacgagc tatccatcgt aaattaaatg aatttcttcg
     1201 tgttgtgatg gatcatcccg atttgacatc gacaatcttg ccattaggtg atggtgtgat
     1261 cttaatgaca aaggaatcag aaacaatcga gttgtgatac atgattatgg cagcctgtag
     1321 ataatcgatt gaatcgaggg ctcaattcga acttgatgta aaagtttcta tatttgtaag
     1381 tcaattaaag gatacaggaa atctaaataa acaggagtag catgaagtca atgtagctcc
     1441 tgttttcaat tgataacctt agaacagctg aaggagaatg tctcgtttta aaagattgat
     1501 ttagtagttg ggccaaaata attcattatt tgctaattaa ataactattt cgtattgaca
     1561 gaagcaattt aattatctat aattattgat gttccgctga ttcttgtcat agtagctcag
     1621 ctggatagag catccgcctt ctaagcggac ggtcgggggt tcgaatccct cctgtgacgc
     1681 taaaaaaccg ttagaaactg aggtttctaa cggtttttct ttgttttttg tgccaatatt
     1741 gtttgttttc aaaggcaaat aaaataaaaa aacatcctta tcgagtacaa ttataaggag
     1801 caaaaaaata aaactcatca gaaaagagga agaagatatg acgatactat ctagaatttt
     1861 agtgactcta gttgcaattg aattttttta tatcatgtat atcgaaacgg tgagaactga
     1921 ttctgacacg acaagtaggg tttttaaaat gagtaaagaa gagttaagca gaaaatctgt
     1981 tcaaacattg tttaagaacc aaggagtgta caatggacta ttaggagtgg gactacttta
     2041 tggagcttat ctttcttccg catctaaaga aatcacaagt atgctattaa tatctatttt
     2101 ttttgttgca ttatatggca gtttgactag cgatcgttgg attttactca aacaaggtgg
     2161 gctagctctg ttggcattgc tttctatttt cttataaata tagaaaaaat aagaatttat
     2221 ctaacaggaa actttgtgaa aaatattact ttatctcatg aaatatgata ttctaatgaa
     2281 tggtatttta gaaaatttca tgaaaaagga gttagatagg tgttcaaaaa ttatgtttcc
     2341 tcactaaaaa aagaattttc aggttatgat ctttcaagat ttcagaaaga tcttttggct
     2401 gggatcacag tagctgctgt agcattgcct ttagccttgg cttttggggt atcaagcgga
     2461 gctgatgcgg ccgcaggttt gattacggct atcattgccg gtatcgttat tggcggttta
     2521 tcaggcggtt tttatcaaat atcaggtccg acaggtgcga tggcagctat cttgatgtct
     2581 attgcggcaa caaagggaat gcaaggtgta ttgcttgcta cttttttagc gggtctattt
     2641 ttacttgcag caggactttt ccgattagga acactgactt cttttatccc agcacccgta
     2701 attacggggt ttacttcagg tattgctatc attattgcat taggtcagat cgataattta
     2761 tttggtgttc actcggaagg tacaaacgtt attgaaaaac taagtagtta tcaaacgata
     2821 ggatttccga taaacacagc ctctttgatc atgggagggc tagtcatttt agggatgttt
     2881 atctatccaa aaaaatgggc gaaacgaatg cctagttcgc tgctggctat cattttggca
     2941 acagcagtta tgctattgtt ccatttacca gtagcaactg tgggtaagat tccacaaaca
     3001 ttgatcagta gtaatcgttt gaatatggga gattttagct tttctgcctt gcaacaagtg
     3061 gcagttccag ctattagcat tgctttatta ggaatgattg aaagcttgct atgtggtgct
     3121 tcggctggcc gaatggcaaa taagccctta gatagcaatc aagaattaat ggcgcaagga
     3181 attggaaatc tgttgcttcc tttctttggc gggattcctg ctactgcggc catcgcccgt
     3241 acaagcgtgg ccatcaaatc tggtgcacaa actagattag ctggtatgat acatgcagtt
     3301 gtcttactgc tctccatgct tgtttttgcg ccagtgatgt tacagattcc aatgccagca
     3361 cttgcaggtg ttttgattgt gacggcttgg cgtatgaatg aatgggaaac gatcaaggag
     3421 ctatttacta ataaatattg gtcagcagtt ctattgttta tccttacgat gatttgtaca
     3481 gttatctttg atttaagtat tgctatcgtg atcggggtga tcggcggctg tgtatttttt
     3541 gttgtcaaaa gcgctgctat cacgatttca ttagaaacga tcgattggca aagaatgggg
     3601 ctgccagaaa cggaaaagct gaataactgg acagtagtgt atattagtgg tccactgttt
     3661 tttatgtctt cagaaaaact caaaaataaa ttggaagaac taaatcatca cgatggtgtc
     3721 attttctcca tgcgaggcgt accaagtatt gatttgacgg cacgtgcagt ttttgatgaa
     3781 tttcaagaaa aagcagagca aagaggacaa acgattatct atacttcctt gcagccagaa
     3841 gtagaaaaac aactttctca cttgtgggaa catacattca cgcatcaaca tcaaacagtt
     3901 gctcatgcgt tgacagcttt acatgcgaaa tatactgcaa ttagccatta aaacaatgaa
     3961 aaagaacaga caagactgta aactgaagat tttatggtct tgtctgttct ttttaatatt
     4021 gctgtaaaag tgaagctagt acttgccagt cttgatggat aacaggtaca agggagcgat
     4081 tgtagaaaat agctgctgga tgaaaaagtg gaagaattcg atagtttttt tctgaccatt
     4141 cgtaggcatc cttttgttct gttaattgta agacaggccc tgtatagagt tgaccatgat
     4201 ttttggtaac cgtatattct ttgccaagta aacgttgaag accaatgttt ccaagtgtcg
     4261 caattaattt tggctggacc ttcatcagtt cgtaatccaa tattggtgca tgcgcaaata
     4321 cttcggaacg agtaggtgtc cgatttggat aaacagtttc tgactgttgt gtccgtttat
     4381 tgatccgatg agtcacgcga tacgggcggc tccttacagc actggtgata tacacctctt
     4441 ctcgtgttaa gccagttgaa gaaagtgcct gcatcaattc ttttccagct tgaccgctaa
     4501 aaggaacatg cgtatcaatc tctgttttcc ctggggcttc tcccacgagc atcagtttag
     4561 gatgaagagg tccttgccca gcgataaatc cttctacagg acggccgttc attcttaaag
     4621 aagcttcttg tatcagttct tttgggtatt ccatttatgc tcctcctttt tcttttatta
     4681 taagaataaa agaaacacaa tgcgactatt ttatttggta gaactgtgag gatagaaaag
     4741 cagaagtatt gcttgaattt ttgttttact ttttgatttt aaatttagtt gactaacggt
     4801 tgctgttctg gtaagctagt actgttgtaa ttgtggactc cacagctaca accgctcaga
     4861 acaagtaata agcacagcat atgctgtcac ttcggatggc gagtctaagt atatcagaag
     4921 gaggtgcttt atagcatgta cgcaattatt aaaactggtg gtaaacaagt aaaagttgaa
     4981 gtgggacaag caatctatgt tgaaaaactt gacgttgaag caggagaaaa agttgttttt
     5041 gacgaagtta tcttagtagg cggagaatct acaaaagtag gcgctccaac tgttgcaggt
     5101 gcaactgttg aaggtactgt tgaaaaacac ggcaaacaaa agaaagtcgt tactttcaaa
     5161 tacaaaccta aaaaacattc acaccgtaaa caaggtcatc gtcaaccata tacaaaagtg
     5221 gttatcgacg cgatcaacgc ttaatagcgt ttgagaaaga aggcctatat atgatcaaag
     5281 gaacatttaa gcgaaatgac gcaggtcaga tcgtttcatt cactttgacc gggcatgctg
     5341 atgctggtcc ttatggaagt gatatcgttt gtgcgggtgt ttctgcgtta gcgatcagca
     5401 ctgtcaacgg gattgcctca ttagccggtt ttgagccgat tgttgaaatg aatgaagaag
     5461 aaggcggtta tctttatacc gaagtcactt caggaatgac acaagaacaa aacaatattg
     5521 ctgagattct attagaaaat cttttattag gattacaatc gatagaagct gaaaactcag
     5581 aatatatcca aattaaaaca attaatgata aataggaggt gcagaccatg ttgttatcta
     5641 tgaatctaca attatttgcc cacaaaaaag gtggcggttc tacatctaac ggccgtgact
     5701 cagaatcaaa acgtctaggt gctaaacgtg ctgatggaca aacagttaca ggtggatcaa
     5761 ttctttaccg tcaacgcggt acaaaaatct atccaggtgt aaacgtgggt atcggtggag
     5821 acgacacttt atttgcaaaa gttgatggcg tagtacgttt cgaacgtaaa ggccgtaaca
     5881 aaaaacaagt gtctgtttat ccagtagctc aagaagctta attgaatctt atgattaaag
     5941 aggttgtgac aagacttttg tcacagcctt ttttgtttat aaaaataaaa gttgtagtgg
     6001 aggctacgaa gtttactttg tctttttaca cttaatattt ttttgccaat ttggaatcag
     6061 gatgttttga taactatcgt tttcaattga tggaagctat attgtttgct atcagtgaaa
     6121 taattatatg gtaaacagtg ttctttttct tgtttccatt ataaaaatcg tatcgggatt
     6181 tttcgttctt ttaaattgaa catttctatc tatccgcgtg ttatcaacaa aacaaaatca
     6241 ggtaaatagt tgacgaaaca gaaataaata catagtatta tgttttgaag cctaagtaca
     6301 atagtctata gaaaaggagc gataccattg gcaaaacgca ttgaccgaat ttatatgtat
     6361 gtcaaagaaa aaaccgctca tttaacaaag gcagaatatg atcaaggagt aacaacacag
     6421 gaaattgctg aagtgttagg gattcagcga acaaatagca gtaaagatct gaatcagcta
     6481 gtgcgcgaag gaaaactgat aaagacagac ggacgaccag tacgttacat atatcaaaca
     6541 ctcgttacgc acgtaaagcc agttacaaaa catgtagtca gttacaaaga aacgccggtc
     6601 tctgaagaaa aaaatacact ccaaatagat actaaggata tctttgcaaa aatcattgga
     6661 gctaacggca gcatgaaaaa ttcagtggaa caggcaaaag ctgcgatttt gtatccgcca
     6721 agaggattaa attgtttgat tacaggacct accggatcag gaaagactta ttttgcacac
     6781 gcgatgtatc attttgcaaa agcgcatcat gtaatagatg aggaaacgga actgattgtt
     6841 ttcaattgtg ctgattatgc caataaccct gagcttttga tgagtcattt atttgggtac
     6901 gcaaaaggtg cttttacagg cgcagatgaa gaaaaaatgg ggattatcga tcaagcggat
     6961 ggcggtatgc tgtttttgga tgagattcac cggttgccgc ctgaaggtca agaaatgatc
     7021 ttttacttta tggatcatgg aacttacagt cgtcttgggg aaacaacgaa atcccatgaa
     7081 gcaaatgtac ggatcgtagg tgcgaccaca gaagacccag gttcttctct attagaaact
     7141 tttgttcggc gtatcccaat caatatcaaa cttccggctt ttgaaaaacg tcctgcaaat
     7201 gagaaagtcg atttagtaaa aatcatgatt gcccacgaag caaaccgcac gcagcgaaag
     7261 atttctttga cagaagatgt agcaaaagca ttgatcggca gtgtgaccta tggaaatata
     7321 ggacaattga agtcaaatat ccagttagta tgtgcacgag gatttctaaa tcatatgcaa
     7381 tcacccgaaa tctcgatcac aatcgatgat ttgacagaag ggattcgtag cggattgatt
     7441 caattagcaa gcaaccgcag tgcaatggca gagctttcta aaatattgga gccaaagata
     7501 actgtagcac caaatgatac gatcatgaaa atccaatcgg actcctatga gttaccttat
     7561 aatctatatg atatcatcgg agataaagca gctttactga agtcggatgg tttggatcaa
     7621 gaagcaatca atcatttcat ttctacagat atcaacatcc atctgaagtc tttctataaa
     7681 gaccatggtt tttcttttaa tgcagacagc aaattagcag aatttgtaga tccgaaaatc
     7741 atagaagtga ccaatcagat ttatgtaatg gtcaaaagat ctttaccata tgagtttcag
     7801 caaaacttta tttatgctat gagcttgcat atcagttctt ttttgaaaaa aatcaactta
     7861 ggagaagaac ggcatacgaa tgataacatc cgtgagatgg cttttgattt tccagatgaa
     7921 tatgctgttg cgaaagaagt gcgtagttat atcgaaacgt attttcaagt ggagattcca
     7981 gaaagtgaag actattactt gaccgttcta ctcgtctcgt tacgggcaaa tcaagcaagc
     8041 ggcagaatcg gtgtagtcgt tgcagctcac ggaaacagca cagcaagcag tatggtccaa
     8101 gttgtccaac agttgttaga tgcagagaac gtacgagcag tagacatgcc tttggatatg
     8161 gatccgaaaa cagcattgga ccgaattgaa cgcaatgtac aagaagtaga tgaaggaagc
     8221 ggggtcatac tcttagtaga tatggggtcc cttgcgtcgt ttaattcgca aattcaaaga
     8281 gacactggaa ttccagtacg tactgttgat atggtaacaa cttctctagt tttggaaact
     8341 gtacgaaagg tctcagtatt agggacagat ctagatatgt tgcatgaatc attgaaaaat
     8401 tttcgaggtt acgcagaaat atcttctgta gaatctgttc aggaagcaaa acctattcat
     8461 aagaaaaaag ctgttttggc tatctgcgca tctggcgaag gtacagcaca aagaatcaaa
     8521 gaattgatcg aacgagcggt aaacaagcgt caagaaacag aattagaagt tctagctttg
     8581 tcgattgtgg aagtgaaaga agaactgcca gttatccaaa aagaatatca gatcatagct
     8641 actactggga ttactgattt gaatatccaa gccccattta ttcctttaga acgttttatt
     8701 gatcaaaata tcgaagtcat tttggatcag ttgctgatgg aatcagaatt ggaggaaact
     8761 gaattcatct ccctagatga agagtcagct aaaaatacgt gtgttgagtt tatctctgat
     8821 aacttcatct ttattaatgg tagtaagtta atcgatccaa tgtggcaatt ttctacacaa
     8881 atctctcaga cgacgggaat cggcgatgaa gaatatggat tcaaaatcaa cctagtgatg
     8941 cacacagcag gaatgatcga acgtattatt cggaatgaac cattgactgt ggaagaaaac
     9001 gaattgacaa atacaacgaa tgatcctctt tatagccaat tggcagcctc agttgttctg
     9061 ttggaagatc agataaaagt aaaagtgccg atagaagaaa tgtattatct gctacgtcta
     9121 gtccataatc agttagataa aaaagaatac actgtgccat gaatacacaa aaacgataca
     9181 cttttatagt gtatcgtttt tgtgtattgg ttaagaatcc tttaaattca attagtgtat
     9241 cgtaaaaaag ttggcacgct ttttgcttat agtagtgtgt gctaggaggt ggttgttcac
     9301 acagaaatga taggggtttc tgtgaagtag tgaggaagaa ggagttttgc agatggatat
     9361 tcgattggca cgtatagatg atcgtttaat ccacgggcaa gtcgcaacag tttggtcgaa
     9421 gcaaacaggt gttgaacgta tcattgtgat cagcgatgta gtcgctcaag ataaattgag
     9481 aaagtttcta ctaaaagaag ctgcaccgcc ggggatcaca gcaaatgtaa taaccgtgca
     9541 gaaaatgatt caaatctatc atagtggatt attatcacga gcaaaagtga tgcttttatt
     9601 caccaatccc caagatgttg aagcggttgt ccgctgcggt gtacagttga agtcgttgaa
     9661 tatcggcggt atgagtttta ctgaaggcaa atcaatgatt accaattttg tttcagtaaa
     9721 tcaacaagat attgattcct ttcgttactt gaacagtcaa gaagtggagt tagagattcg
     9781 aaaagtacct gctgacagga aaatcctttt gatggaacta ctagcgaaaa tcaaaaaggt
     9841 gtagcaagaa tggttgttgg aaaaggtcaa ataattctgt tgtaatggtt agcaattatt
     9901 aatatttttg aatttgttac aacaaaagta aataggaggt ataaatatgg tagggattat
     9961 cctagcaagt catggacaat ttgctgaagg aatcttacaa tccggttcaa tgatctttgg
    10021 cgaacaagaa aacgtcaaag ccgtgatttt gaaaccaagt gaaggtccag atgacttgag
    10081 agccaaattg gaagaagcgg ttgcttcttt tgataatcaa gacgaagtgt tattcttagt
    10141 tgatttatgg ggcggaacac cgttcaatca atcaaacaca ttattcgaag agcacaaaga
    10201 taaatgggca atcgtcagcg gtttgaattt accaatgttg attgaagcct acgcatcacg
    10261 cttttcaatg gaatctgctc atgaaattgc agcacacatc attgaaacag caaaagacgg
    10321 tgtaaaagta aaaccggaag aattagaacc ggcagaagca ccgaaagcag cagttgaaga
    10381 cgcacaacct aaaggagcac ttccagaagg aactgtggtc ggagacggaa aaatcaaata
    10441 tgtattagca cgtgttgact cacgtctgtt acatggacaa gttgcgacag cttggacaaa
    10501 agcagtgcaa ccaaaccgta ttatcgttgt ttcagatgca gtatcaaagg atgatctgcg
    10561 taagagacta atcgaacaag ctgctcctcc aggtgtaaaa gcaaatgtta ttcctattag
    10621 caaaatgatc gaagtagcaa aagaccctcg ttttggaaat accaaagcat tgcttttatt
    10681 tgaaaatcct gaagacgtat tgacagctgt tgaaggtggc gtagacatca aagaattaaa
    10741 tgttgggtca atggcgcatt cggtcggtaa agtcgttgta agtaaagtat tgtctatggg
    10801 acaagaagac gttgaagcgt ttgagaagct tgaacaaaaa ggtgtgaaat tcgatgtacg
    10861 taaagtacct aatgattcac gcgataacat ggatgatatt ttgaaaaaag caaaagctga
    10921 attagcaaaa gcgtaataaa taatattaaa atttatagga ggcttatcat gtctattatt
    10981 tcaataattt tagtcgtatt agttgccttt ctagctggta tggaaggtat tcttgatgaa
    11041 tttcaatttc atcaaccact agtggcatgt acattgatcg gtttagttac cggtaacctc
    11101 gaagctggta tcgtacttgg cggtacgctt cagatgatcg ctttaggttg ggcaaatatc
    11161 ggtgccgctg tagcaccaga tgctgcatta gcatcagtag catcagcaat tattttagtt
    11221 ttaggtggac aaggtgttaa aggtgttcct tctgcgatcg cgattgccgt tcctttggct
    11281 gttgcagggt tgttcttaac aatgatcgta cgtacaatcg ctgttccaat cgttcatttg
    11341 atggatgcgg ctgctgaaga aggaaacatc cgtaaggttg aaatgtggca catcattgct
    11401 gtatgtttac aaggggttcg tatcgcggtc ccagcagcag cgttgctatt tatcccagca
    11461 caaactgtgc aatcattcct tgaatcaatg ccagcatggt taacagatgg tatggctatc
    11521 ggtgggggaa tggtcgtagc tgtaggttac gctttggtaa tcaacatgat ggctacaaaa
    11581 gaagtatggc cattcttcgt tattggtttc gttgtagctg caatttctca attaacattg
    11641 attgctctag gtgctttagg tgttgcatta gctcttattt acttgaacct ttctaaaatg
    11701 ggtggttcat caaataatgg aggcggaagc aactctggtg accctttagg cgacattttg
    11761 aatgactatt aatcttgaag gaggaaaata aaatggcaga agaaaaaatc aaattatcaa
    11821 aaaaagatcg tttagctgtt gcgtggcgct ctaccttcat tcaaggttca tggaactacg
    11881 aacgtatgca aaacggtggc tgggcatttt ctatgattcc agcaatcaaa aaattataca
    11941 aaacaaaaga agaacgttct gcagcattaa aacgtcactt ggaattcttt aatacacacc
    12001 catacattgc ttctccaatt ttaggggtta cattggctct ggaagaagaa cgtgcgaatg
    12061 gcgcgcctgt tgatgatgta gcgattcaag gggttaaagt aggtatgatg ggtcctttag
    12121 ctggtgttgg tgacccagtg ttctggttta cagtacgtcc aatgctaggt gcactaggtg
    12181 cttcattagc tatgggagga aatatccttg gaccaatcat cttcttctta ggttggaaca
    12241 ttattcgttg ggcattcatg tggtatacac aagaatttgg ctacaaagct ggttctaaaa
    12301 ttactgatga cctttctggc ggattgttac aagacgtaac aaaaggtgct tcaattttag
    12361 ggatgttcgt attagctgcc cttgtacaaa gatgggtatc aatcaaattc ttaccagttg
    12421 tatcaactgt aaaattagat aaaggtgctt acatcgaatg ggataaattg ccttcaggtg
    12481 gtgaaggaat caagagtgca tttgaacaag ttaataatgg cttagccctt tctcctgaaa
    12541 aggtaacaac attacaagac aacttagacc aattgattcc tggtttagct gcattaggat
    12601 taacattgtt atgtatgtgg ttgcttaaga aaaaggtttc tccaatcgtt atcatccttg
    12661 gtttattcgt agtcggtgtt gttggacacg tcatcggact tttataatcc ataaaaatat
    12721 agctcgggcg ttgtcccgag ctttttttat atagtatact gttcataaac aaatatctgg
    12781 agggagaaaa tggttcaatc aatcaataca aaagtagact tggtaattga tgctacttcg
    12841 tttacgggtc tgacagatta cgggcaaatc atgataggtg ataatggttt tgagttttac
    12901 catgcgcgag atagacgcaa atatatccaa attccatggg aagaagtgga ttatgtacta
    12961 gcatcagtca tgctaaaggg aaaatggatt cctcgatatg ccattcaaac gaaaaaaaat
    13021 ggtacataca cattttcttc caaagaacct aaaaaagtgc ttcgtgcgat cagagaatat
    13081 gtagatgaag atagaatggt acaatcattg agtttctttg atgtagtgaa acgtggtatt
    13141 cgtagtatgt ttaaacgcaa gtaaaaaata aagaaaactt ctaagaggag gttttcttta
    13201 tttttttact ttgcagaaga aaatatgaaa tagcaaaacc aattatttgt cccctttaaa
    13261 tcccatatgc taaaattgat tagcaaagaa ggaggttata ttatgagttg gttatggaca
    13321 ttgattgttg gagccgtgat cggagcaatt gcaggtgcga tcaccagtaa aggaaattcg
    13381 atgggctgtg taacgaatat cattgctgga ctagtcggtt caagtattgg acaagcgatt
    13441 ttcggcgaat ggggaccaaa agctgcaggt atggcactta ttccatctat tttaggcgca
    13501 gtgattttgg tagcagttgt ctcatttttc tttggaaaac gatcataagt ttttacaaaa
    13561 tagtaaacta aaaggagttg cagcgagact gtgactcctt tttggcttta tttatttaaa
    13621 ataaaaagaa gttttccgga aatttttatt taactgtcgt gtacgaagaa aagtttgcta
    13681 taatggaaaa aagcgtgcaa agaaagaggg agtttcatta tgatgctacg tgtagaaaaa
    13741 ttgcgcaaaa aaatgcaaga agaaaatttg gattcatttt tggttaccag tccgtacaat
    13801 cttcgttact taacgaactt tacaggtacg acaggattag ctgttattac tttggaaaaa
    13861 gcatttttta ttactgattt tcgttatacc gagcaggcgg cagcgcaggc tcaagggttt
    13921 gaaattatta aaaatgtcgg acctattttt gatgaggtag caaacttagt acgcaaagaa
    13981 gaactgcact cattagcttt tgaggaaaca acagtctcat ttttagaata ttcggtttta
    14041 gaagaaatca tcgatgcgga attagtacct gtttcacaga tgatcgaaga actcagagaa
    14101 gtgaaagatg aagaagaaat cgctattatc gaaaaagcat gtcatattgc ggatatggca
    14161 tatgatcaca tcctgaaaat gatccaaccg ggcatgactg agattgaagt cgcaaatcaa
    14221 ctagactttt atatgcgttc tcttggcgct tcaggtgttt cttttgatac gatcgttgct
    14281 agcggattgc gttcggctat gccgcatggt gtagcaagca aaaaaatcat cgaacaaggt
    14341 gacttgatca cattggactt tggttgctat tacgaagggt acgtttccga tatgacacgt
    14401 acattcgcta ttggtgatcc tggggaaaaa ttaaaagaaa tttatcaaat agtcttagat
    14461 gctcaattag cagttattga tgctgcaaaa ccaggaatga ctggtgtcca attagatgca
    14521 gttgctagag attatatttc aaaacatggt tacggggaag catttggaca ttctacagga
    14581 catgggatcg gtttagagat ccacgaagga ccaaacgttt cttctcgtgc agaaaagcaa
    14641 tttgtaccag gaaatgtgat cactgatgaa ccagggatct acttaccagg aatcggtgga
    14701 gtacgtattg aggatgactt gttgatcaca gaagatggaa atcgtgtgtt gactcgttca
    14761 ccaaaagaat tgatcatctt atagtttcat caatagaaga agtaagcgta aaaatcagtg
    14821 ttcacagact ttgttataga aaatcactcg atttaatgat acactataga agaactttta
    14881 agctgtaaga gataataaag ttgggtgttt tctccaattc atagcagttt gcagctgatc
    14941 ggcggcagtt ttcttaccac gtgatcacta tgccgcatca tagagaattc ggatgtatct
    15001 cgtgcttagg caaagggagg aataaataaa tggcagatga aaaaaatttg attttaaacg
    15061 caaaccaaga tttaggagag atcgtgatcg ctcctgaagt catcgaagta attatcggga
    15121 ttgcagcttc taaagtagaa ggcgtatacg gaatgcgcgg aacttttgcc agcaatgtca
    15181 ctgaattatt aggacgtgca gctcacggaa aaggtgttta cttagcaaac gaggaagatg
    15241 gcttgaaagt agatctttac tgttatctag aatatggtgt ttcagttcct aaagtagcaa
    15301 tggaaatgca ggaacgggtc agccagcaag tccttttcat gacggatatc gatttatctg
    15361 aagtcaatat ccatgtagta gctgttgttc ctgaaaaatt agaacaacca acaatcgaag
    15421 aactttttga agacgaagag gaagagaatg aataaagaat tatcaagaca cgagattcgg
    15481 gaaatggcat tgcaggcttt atttccatta gacttcaatg cagacctaac aaaagaagat
    15541 gcgattttca acgcaatcga attagaccac cgcgacatga tcaacgaaga tgaatcagag
    15601 tttgttcctg tttatcttga tacgctagtt ggcggtgtct gtgcaaaaaa agatgaattg
    15661 gataaagtga ttgaaaaaca tttaaaaaac aattggcaca tcaatcggat ctcaaaaatg
    15721 gatttggtga tcttgcgtat cgcgattttt gaaatgactt atgtgacaga tgtaccagcg
    15781 gctgttgctt taaatgaagc aatcgagttg gcaaaaacat ttagtgatga ccgttcacgt
    15841 aaattcgtca acggtgtttt atccaatgta ttgaaagaat tagaagctgg ggcgtaagtc
    15901 ctagcttttt tctatgaatc atcataaacc tgttataaat cgggaaaatt ctatggtaga
    15961 atgaaaacac aaaaaatatc tgggagtggt tagatggcag aattgattaa cggaaaagaa
    16021 ttagctgaaa aaatgcaggc agagattgct gaaaaaatcc aaaaattgaa agataatggt
    16081 atccatcccg gattagtcgt tttattggta ggggaaaatc cagccagcca aatctatgta
    16141 agaaataaag aacgtgtagc aaaagaaatc ggaatccatt cattggtaga gcgttatcct
    16201 gagacgattt ctgaagacga actacttgca gaaattgaaa aatacaatca agatcctagt
    16261 tttcatggta tattggtcca attaccattg ccaaagcata tagatgaaga aaagatcctg
    16321 ttagctatcc gccctgaaaa agacgtagac ggttttcatc cgatgaattt aggcagactc
    16381 cttgcgggta aaccggacaa gattccttgt actccatacg gcatcatgaa aatgtttgaa
    16441 gcttattcta tcgatccggc gggtaaacga gcagtagtta tcggacgaag caatatcgtc
    16501 ggtaaaccga tggctcagct actcttgatg gcagatgcaa ctgttactgt tgctcattct
    16561 aaaaccgaaa atctggctga actggcacaa gaagcagata ttttagtcgt agcaatagga
    16621 cgtggacatt ttgtcacaaa agaattcgta aaaccaggtg ctgtagtgat agatgtaggg
    16681 atgaatcgtg atgctaacgg taagctgatc ggtgatgtga aatttgatga agtggagcct
    16741 attgcaggct ggatcactcc tgtgccaaag ggtgtagggc caatgactat cacgatgctg
    16801 atgtatcaaa ccgtaaaatc tgccgaagct tttggagcag gtgaatagat ggcacaagaa
    16861 tatttaactg tcactacatt aacgaagtat ttgaaaagaa aatttgatgc cgatccttat
    16921 ttagaacgcg tatatttaac aggcgaaata tcgaatttcc gtttacgtgc caatgcccat
    16981 caatatttca gcttaaaaga tgaccatgca aaaatatctg ctattatgtt caaaggagca
    17041 ttccaaaaat tacgattcca gccaaaagaa ggaatgaaag tccttgtcgt tgggcgtatt
    17101 tcattatatg aagcgggagg ttcttatcaa atctatgtag agcacatgga gcctgatgga
    17161 ataggtgctt tatatcaaga attggaagaa cgaaaagaaa agctgatgaa ggaaggcctt
    17221 tttcaagggc ctaaaaaaat gctgccacgt ttcccaaagc gaatcgcggt ccttaccagt
    17281 ccaagcggag cggttatccg cgatatcatc acgacggtga aaagaagata tccaattgtc
    17341 caattggttt tgttcccaac agttgtacaa ggaaaccaag cagcggacaa tgttgtcagg
    17401 aatatccaga gagtagaaga gatgggcaat ttcgatacaa tgatcatcgg tcgtgggggt
    17461 ggttcgattg aggatttatg gccgtttaat gaagagcggg ttgctcgggc gatttatcaa
    17521 gctaatacgc cggtgatttc atctgtagga catgaaacag ataccacgat cgctgattta
    17581 gtagcagatg ttcgggctgc cactccaaca gcagctgcag aattagctgt tcctgttttg
    17641 tcagaagagt tgatgcgcat tcaagaacgg caagctagat tgcagcaggc gtttgtacgc
    17701 cagatccagc gcaaacaaga acggttcgag cgtgctcgac gttcctatgt atttcggcag
    17761 cctgaaagat tgtatgaagg tcagaccatc aaattagacc agttgaaaca gcgattatat
    17821 caagctacac agcaaattta tcatgcgaaa gaaaaacaaa cgatcgcatt aagccaccaa
    17881 ttggaacaag tggctccgct ttatcgagtt aaagcagctc gccaagaaac tgcttatcta
    17941 gaaaaacgct tagaagagaa aatccaacaa tatatgaaac aacagcagca gcgttttgct
    18001 caagctgttc aatctcttga tttgttgagt ccattgaaaa tcatgggaag aggctacagc
    18061 tatacgacgt taaatgatca agtagtaaaa agtgtcaccg agattcatca aggggatttg
    18121 ctgacgatcc attatcaaga tggaatcgtt aaaggacaag tacaagagat agacaaagga
    18181 gaataaaaat atggaaaatc caacttttga agagtcactg caagaattag aaaaaattgt
    18241 catgcagtta gaacaaggag acgttccatt agaatcagct ctagattcgt ttaaacgagg
    18301 aatggaatta agcaaacatt gccaagatac attagctaaa gcagaagaga ccttgacgaa
    18361 aatgatgaca gaatcaaatg aagaagttga ttttgacgga aacgaggaaa gctgagatga
    18421 gattaactga cttttcagct attcatctac ctgctgtaga aaaagaaatc ttgtcttttt
    18481 tagatgagca tacaacggat agaggactct atgatgcgat gagttattca gtgaaagcag
    18541 gcgggaaaag aatacgtccc ttattacttt taacggccgt tgcttccttt gatgagccta
    18601 ttgatgttcc cgtctatcaa gtagcagctg ctttagaaat ggtgcatacg tattccttga
    18661 tccacgatga tttgcctgcg atggacaatg atgatcttcg tagaggaaaa cctaccaatc
    18721 ataaagtatt cggcgaagca ttagctatat tggccggaga tggtttgctt acaggagcat
    18781 tccagctaat cagtatggca cacttaggta attctcctaa gcttctgctg cttcagcaat
    18841 tagctgtttg tgcgggatca caaggtatgg ttgctgggca agctgcagat atcgagggcg
    18901 aatcgaaaaa gctttcttta gaagaattag catttattca tgaaagaaag acagggcatt
    18961 tgattcgtta tgccttgctg gctggtggca tattagcgaa gcaacctgaa gaaatcttat
    19021 tgctacttca acgattagcc gaacatttag gtttagcttt tcagataaga gatgatctac
    19081 tcgatgtgat tggtacaacg aaaacattag ggaaaacagc tggtaaagat gaacgtatgg
    19141 aaaaaaatac ttacccgcgt ttattaggcc tagaaaagac acgtgaggcg ttggagattg
    19201 agctgttatc cgccaacaag atcatagaca aattagaaga ggacgttttt tcttttgatg
    19261 gcgggctgat gcgccagatg atcaagcaat ttgatgtgga gaaatgaggg aaagaatgga
    19321 aaaagaacgc gtagatgtat tagcggtcaa acaaggatta tttgaaacaa gagaacaagc
    19381 caaacgttca gtgatggctg gtttgattta caacgaaaaa aacgagcggt ttgataaacc
    19441 gggagaaaaa attccagtga gtagtgaatt aagagtcaaa ggtaaaaaac ttccttatgt
    19501 ttctcgaggg ggattaaaat tagaaaaagc actcaaagca tttgatctat ctgttgaagg
    19561 aaaaacattg ttagatattg gtgcatccac tggcggattt actgatgcag ctttgcaaaa
    19621 tggcgcaaaa atgagttatg ctttagacgt tggctacaat cagctagctt ggaaaataag
    19681 acaagatcct agagtcgtcg taatggaaag agtgaatttt cgttatgcaa aacccgaaga
    19741 ctttacctta ggaattccag aagttgctgt tatcgatgtt tcttttattt cactaaaact
    19801 gatgctgcct ccattacatg ctattttaaa agaagaggga gaagtgattg ctttaatcaa
    19861 accccaattc gaagcaggca gagaagctgt agggaaaaac ggaattgttc gtgaccctca
    19921 gacccatcaa aaagttttag aagacatttt gtcatttgcg gcacaaggtg gatatgatgt
    19981 gctggaatta agttattctc cgatcacggg gggagaagga aatatcgagt tcttggctca
    20041 cttgaggaaa gtacccgaaa gcggaacaat caattcagct attaatatgg cagaagtggt
    20101 ttcaaatgca cacgaacagt ttgaccataa ataaattgta actgtgaaaa tcaagtgaga
    20161 agtatgcgga ttcttcttta ttataccaag gaaataagct atcattgatt aagaagaata
    20221 tgctaaggag cgaagacgta atgagaaaaa aagatcggca tcgtttgatt actcgattgt
    20281 tgagtgagca agatatccga aagcaggaag aatttgttga aatcctgaaa agcaaaggaa
    20341 tatctgttac tcaagctacg atttccagag atataaagga actgaaactg atcaaggtac
    20401 cggcttcaga tggcggctac cgttatagca taccagctga aacaaacgaa gatgtaaata
    20461 ctaaattgga aaaactatta aaagacgctt tcgtggcagt tgaccaaatg gaaaaatttg
    20521 ttattttgaa aacattgccg ggaaatgctt cggcagcagc aaatttgatt gataaacgtt
    20581 acaagaaaga attattttcg attatcaatg atgacgataa tgttttgatg attacacgaa
    20641 ctgaagacca ggcagtttta ttgaagaaag acttcctgca ttacctttaa gttgaggagc
    20701 gaatgtgatg ctacaagaaa tcagtattac taactttgcg attattccgg aattgcggct
    20761 ttcttttcac gaaggaatga ccgcgctgac cggagagact ggtgcgggta aatccattat
    20821 tatcgatgct ttaggtctgt tagcaggagg aagaggttca agcgattata tacgtcaagg
    20881 agcagaaaaa tgtgtgcttg aagggttgtt tgaactacca aaacaagaag gattctctga
    20941 actgatggtt gaattaggga tcgaaacaga tgaggacaat ctgattgttc gacgggatat
    21001 gtccttaact ggaaaaaatg tttgtcgagt gaatggtcat attatcacat tagctaatct
    21061 gagaagaatc ggcagttatt tagtagatat ccaaggacaa aacgaacatc aggaactgct
    21121 ccagcctgaa tcacatcttg ctttattgga tcgttttgga gatgtagctt tccaacagaa
    21181 gaaaaagagt tatcagcaag aatatatctc ttatcgtgag ctggaaaaaa gggtacgaaa
    21241 agtgcagcaa aatgaaaaat cctatgttca gagaatagat atgctccatt tccagcaaga
    21301 agaaatagct gctgctgatt tgcaagtcgg agaagaagag aaattaaaag aagaaagaga
    21361 aaaacttagc aattatcaaa aaattgctga tggattagct gcaggttacg gagcactgac
    21421 gaataatgaa caaaacagtg tagatggtgt tggcttggcc gtttcagaac tgcaagggat
    21481 cgcccatcta gatgtggagt atgaagcaat ttatgaaaat atccaaagtg cttactattt
    21541 gctgcaagat gcaatcggcg atatgagtcg tcagattgat ttattggagt tagatgagag
    21601 tcgtttagaa gaagttacgc aacgtttaga attaatccga cagttgaaac gtaaatacgg
    21661 cgaatccatt gagtcaatat tggcttacta tgacgaaatc accgaagagc tagcttcttc
    21721 tgatttttca gaaggtcaat tggacaaaat gaaagaggaa ctagagcaaa aagagcttct
    21781 acttcagcaa caagccgcag atcttcatga agcgcgtaaa aaaatcgcaa aagaattgga
    21841 aaaatccatt ctgcatgaat tgaaaagtct gtatatggaa aacactgagt ttgaggtacg
    21901 tttcttaaaa gaagaaaata gacaattgaa tcatgatgga tttgaccaaa tcgaatttta
    21961 cattacgacc aatccaggag agccactgaa gcctttggtg aaagtcgctt ctggtggcga
    22021 actgtctcgg atgttgcttg ctttgaaaac tattttttct tctgagcaag gtgtgacaag
    22081 tattattttt gatgaagtgg atacaggtgt gagcggaaga gttgcacagg ccatcgcaga
    22141 taaaatttct caaatatcga aatactctca agttttgtgc atcactcatc tgcctcaagt
    22201 tgctgcagtc gccgactatc aatattatat tgtgaaagca gtgattgatg gacgtacgca
    22261 aacttctgtt tcagagttga aaacaaaaga aagagaagaa gaaattgcac gtatgctggc
    22321 tggaagtgag attacaaaac tcacactcga acatgcaaaa gaactattga aactcgcaaa
    22381 aaggacctga gccacctcgc agtggttcag gtccttttca gcagctgata gctatatttt
    22441 atttttacat gacactcatt acagcagtca atacaacaga tccaatcgtt aaaatcagca
    22501 ttgtccaaat gacaaatttt gttactttag caaaagtact tgtttgtttt ttttcacgca
    22561 ttgttttcaa cctcgtcatt aattagttga ttcaagtgta caatcttttt tccatttcca
    22621 caagagggct tttttataaa aacttccctt tttttaaata ataggtagtt acgatacaaa
    22681 taaataatgt taatagaact agccatataa aggcatcatc cctttcagca ataggtaact
    22741 taatgttcat tccatagata ctgccgacga ttgtcggtat agttaaaata atcgttaaag
    22801 aagttaaaat tttcataata ttattcagat tattcgatac gattgctgaa aaagtctgat
    22861 tcatttgttc tactagtttt aattgaatgc gagaagtagt cattgcctgc tctgtttcca
    22921 ccaatacatc acgtaaatga tttttcaaag catgattttc ttggaaaagt ttattttgcg
    22981 ctagttcttt caatgcatct aaattagcag aagtcgcttc tctgaaataa acgaggcttt
    23041 tttggatatc catgatttga taaagttgtt tgttttcagt ggatacttga agtttggctt
    23101 ccaaagtatc gcattgagat tgaagcgtag ctaaataact gttgtaagag agtgtgacct
    23161 gccaaatgaa ttgaaagaag atattgagat taggcgaaat atcacttttt ggaaaggtct
    23221 ggctaaagac tttctttaaa aacagtggtt catgattcgt aattgtgata agcttctttt
    23281 tgggcgtcaa aataatagag agcgggtatg tatccacttg aaaataaccg ctaggacttg
    23341 ttttagcaaa aggatattgt aacaataata acgctggctt tgtcaacttt gattgattga
    23401 attcttctgt acgggaattt tctgcatcgt ccaatatgga agtgatgtaa tctgacggaa
    23461 tttcaaattg atgtgtcagc tgatgaatct cttcttgatt aggacgttct acacaatacc
    23521 agatcgtttg gtcagtttcc tttccggtat tacgcagttt ctcattatct aatgtaaaat
    23581 atttgatcat gattttcctc ctgttgcctg tttcttttat tataacggat agggaattgg
    23641 aatacatgga atgtgtttgt aagatataga atataactgc ataaatttaa ataaaatgaa
    23701 acagttgtgt aaagtatata cagtatactt gaagcaacga taaaaaagga gtttacgttc
    23761 atgggcacat tcacaccact tatcttgttt tggtatattt acccggtaat cgttttgttt
    23821 ggttgccaat ttcttgtaaa attattttcg ttaaatcgtc gttttaaaat aaaagcccct
    23881 gatttggcga ttcctttttt gtggggcggt atgcatgcat tgtctagaaa tacagggaca
    23941 atttctatct taccgttttt ctttatttct gttttgctaa tgggaatttg tattgctgtt
    24001 ttccaagcat attattatga agaaattatt tatccgcgtt atttcaagat gacttggcgt
    24061 ttggtttttc tgttgacaat cgtcttgtac gctgtcttga tcattgtgaa catactttct
    24121 tacctatgaa aaaaatagaa aagctgtaaa aagtctttta aaaggctttt tgcagcttga
    24181 tttttaaaaa aatgtttatg aataaaaaaa gttgttcccg gatttcccag aaaaggtggt
    24241 agaaagtggg ggattgtggt agactaattt tagttagtgg aggaatgggg ggctttcaat
    24301 gttgatgggt gaattccaac ataatattga tgcaaaagga cgtttaatcg tgccctcaaa
    24361 acttcgtgaa gaacttggcg aaaagtttgt tctgacacgc ggattggatg gctgtctttt
    24421 cggctatccg atgagcgaat gggaaaatct ggaagctaaa ctgaacgaaa tgccgttggc
    24481 taaaaaagac gcacgtactt ttgtgcgatt cttctattcg gctgccactg aatgcgaact
    24541 tgataaacaa ggacgaatca atatacccag cacgttaaga aattatgcag ctttgacaaa
    24601 aggttgcgta gtcatcggtg tatctaaccg aattgaaatt tgggatgaga cacgatggca
    24661 agatttttct gcagcagcag aagagaactt tgacgaaatt gctgaaagta tgattgattt
    24721 cggactataa gaataggaag aggctgacta atgactgaaa catttcaaca ctatacagtc
    24781 atgttgaaag aaacggtaga tggattgaac atcaaaccgg atggcgttta tgtagactgt
    24841 acactcggcg gtgccggaca tagtgaatat ttattaagcc aactaagttc taaagggcat
    24901 ctgtatgcgt ttgaccaaga tcaaaaagca atcgatcacg ccaagctgcg tttagcttct
    24961 tatgtcgaac aaggtatggt cacatttatc aaagcgaact tcagagaatt agaagaacgc
    25021 ttgagtgagc atgtagataa ggtagacggc attttgtatg atctaggggt ttcctctcct
    25081 cagctagatg aagcagaacg tggatttagt tatcatcaag atgcaccgtt ggatatgcgc
    25141 atggatcagt cagctccttt atccgcttat cacgtaatta atgaatattc ctaccatgaa
    25201 ttagtaaaga ttttcttccg ctatggcgag gagaaatttt ccaaacaaat tgctcgtgaa
    25261 atcgaacgag tacgaaaaac aaaaccaatc caaacaactg gtgagcttgt cgaaatcatt
    25321 aaacaagtca tcccagctcc cgcaagaaga aaaggcgggc atcctgcaaa aagaattttt
    25381 caagctgtgc ggattgcagt aaatgacgaa ctcggtgccg aagaggcctc tttagaacag
    25441 gcaatccggt tattgaaagt caatggaaga atcagcgtaa tcacattcca ttcattggaa
    25501 gacagaattg tcaaaagtat gtttaaagaa tatagtacgg tacaagatct accaccggga
    25561 cttcctgtca tacctgacga attccaacca gaactgaaag tcattactcg taaaccaatc
    25621 cttccttcag aggaagagct aacagaaaac aatcgatcac gcagtgcaaa gttacgtatt
    25681 gcagaaaaaa taagaatgaa agaggagtag aatgatggca gaattaaaga aaatgcaaga
    25741 ttacgaatat gatttacctg aattggtgga tcagccggaa caacaaatgc ctgattcttc
    25801 ttcgaatcaa gaaatcattc ttccacaatc accggcaaaa cgattaaagc agatttctgc
    25861 tttggaaaaa attgctgtgg caagcgtggc gattgctatt gttgctttat gtgttttaac
    25921 agttatgctg cgcacaaata tcagcggagc tgaaaaagaa atcacgtcaa tacaaacaga
    25981 aatcaatgat aaaaagcaag aaaaaacgag tttagaacaa gaaaaaaacg aactttctcg
    26041 tactgaacgg attaaacaaa tcgcagaaga aaaaggattg tctattaatg acgacaattt
    26101 aaggaaagtg aagtaaatga gtcttaaaaa taaaattcgg cggtttatgg aaaagaaaaa
    26161 tctaaatccg atgaacaatc gcaaaaaagt aggtatcatt ttatttgcta cgagtattgg
    26221 attgttcttt ttatttgccg tccgtttctc gtatatcgtt attggaggtc atgttgctgg
    26281 tacctcatta gctgaaaaaa caaaacagtt gtaccaagga agtgaagtag taaaagcaaa
    26341 aagaggaacg atttatgatc gtaacggtgt ggcgctcgca gaagatgcct cctcttattc
    26401 aattaaagcg atactctcaa aaacatatac ctctggggat aaaaaactgt atgttgaaga
    26461 aaagaatttt gataaaatag cagaaatcct gcataagaat ctttctatag acaaaaaaga
    26521 tgcactgaat attttagagg acggcgcaaa aaaagagctg tatcaagttg aatttggtag
    26581 ctatgggaaa aatatctccc aagaaaccaa gcaaaacatc gaagcggaca tgaaaaaaga
    26641 aggtgtcgct ggtctttatt ttgtcgacca tcaggctcgg atgtatccaa atggcgtctt
    26701 ttcttctcat tttattggat acgcagttcc agacaaagat gagaatggct tagttggtaa
    26761 actaggtttg gagtctgctt acaatgatat cttgagcggt aaagatggta agattattta
    26821 tcaaaaagat aattttcaaa atcctctgcc aggtacagtg gcagaagaag aaaaagctgt
    26881 cgacggacaa gatatctaca cgactttaga cagcagacta caaagttatt tagaaacact
    26941 gatggatcaa gtaaatgaag aatatcagcc agaagaattg acagcagtcc tgatgaaagc
    27001 aaaaaccgga gaaatcttgg caatgggaca acgacctact ttcaatcctg aaacaatgga
    27061 aggattgact ggaaaagatg ctatctggcg taatttcttg gtacaagata gttatgaacc
    27121 aggatcaacc atgaaagttt tcactactgc agctgctatc gaagaaggag aattcaacga
    27181 gaatgaaacc ttccaatctg gaaaaatcca agtagaagat gctacgatca atgaccatga
    27241 ctttggtgaa aaaggtgtgc tgaccatgcg tcaagctctt tcatggtcaa gtaacgtcgg
    27301 gatggtcata ctggaacagc gattaggcgg acgatggtat aactatttac aaaaactggg
    27361 atttggacaa agtactcatt ctggattaga tgatgaagta aacggtgctt tacctacatt
    27421 aaatattgtc gaccgagcaa tgagtgctta tggacaagcg gtaggtgtga cgaatttcca
    27481 aatgatgaaa gctttcactt ctattgcgaa taacggtaca atgattcaac ctcgatatat
    27541 cagcaaagtg gtggatcccc aaactggaga agagcgaaca actcaaacag aagttctagg
    27601 gcaacctttt tcaaaagaaa caacagaaaa agtccgtgaa tatatgagag atgttgtcga
    27661 aagcgaaaac tacggtagta cttatggtgt atatagtgta cctggataca atgtatcggc
    27721 aaaaaccgga acagcccaaa tcgcttctga tacaggggga tatcaaacag gagatacagc
    27781 ctacctgtat tcgattgtgg agatggttcc ttctgaagat ccagactatg tcttgtatct
    27841 aacaatgaaa caccctaaga catatgatcg aatggcattg gctaaaatcg caaatccatt
    27901 gatgaaacgg gcaatggatt ttaaagaaac tgaagaagat tcagatacag aaacaaaaac
    27961 agaaaaagta tccgtagccg attatcgtaa tcttgaagcg gacgttgcag cagctgatgc
    28021 tcaaaaaagt ggactgcaac ctgtagtgat cggtaatggt aaaaaagtac aaaaacaatc
    28081 tactgccaac ggtgatcagt tgatctctgg agaaaaactg atcctatata ctggtggtga
    28141 taagctgatg cctgacgtaa caggatggtc aaaagcagat atcatgaagc tggggaaaat
    28201 cctcggtatt gaagttagtt ttgatggaga tggttattgt gtcaaacaag aacttgctcc
    28261 atatgaaaaa atcactgaag acaaacttag ttttacttta gaagaataag aagagaaata
    28321 ggagagaaaa gagcatggaa tggacacaag cattgattcc cattgtcagc agttgtgcaa
    28381 tgacaattgc cgcaatgccg ctatttattg gttattttca aatgaaaaag caaggtcagg
    28441 cgattcgtga ggagggacca aagtggcaca atagcaaagc cggaactcct acaatgggcg
    28501 gcttagtgtt tctgatcggc tctattttga caggaatttg ggttggagct tggcaaaaac
    28561 agctaactcc tactcttttc atcttgcttt ttgtattagc gttatacgga gtaatcggct
    28621 ttcttgatga ttttataaaa atattcaaga aaagaaacat gggactgaac tcgaagcaga
    28681 aattgattgg acagattcta gggggaatca tcttttatct tgtttaccgt tctgaaggtt
    28741 atccaggtgt attgaatttc ttcggactag atctaccttt aggaatcatt tatggtattt
    28801 tcgctctctt ctggcttgtt ggattttcaa atgcagtaaa tctgacagat ggcatcgatg
    28861 gattagttgc tggcttagga agtatctctt ttgcagctta cggtctaatt gcttggcatc
    28921 agcatcagta tgatgtatta atcatttgct taagtgtttt aggaggttta ctgggtttct
    28981 tcgtctacaa cagaaaacct gccaagattt ttatgggaga tgtcggttca ttagcgttgg
    29041 gcggactttt agcggcaatt tctataatgc taaaccaaga atggacattg cttttgattg
    29101 gtttgatcta tgttatggaa acagcaagtg tgatgcttca agttacttcc tttaagctta
    29161 caggaaaacg aatttttaaa atgtctccaa ttcatcatca ctttgaaatg tgcggatggt
    29221 cggagtggaa gattgataca gttttttggt tgatcagcat tgtgacttcg ttgatcacat
    29281 tatggatcgt ttggtaggag atgacgggaa atgaaaaaaa tatcaacata tgaaaataag
    29341 aaagtgcttg tcttaggact cgcaaaaagc ggagtaagtg cggccaaatt attacatgaa
    29401 ttgggcgcac ttgtcaccgt taatgatgga aaaccttttg acgaaaatcc ggaagcacaa
    29461 gagctgcttt ctttagggat aaaagtaata acaggtagtc atccaatcga attgttggat
    29521 gaagaatttt cattgatggt caaaaatcca gggattccct acagtcatcc tttagtagca
    29581 aaagcccaag aaatgggtat tccggtaatt acggaagttg agctagctta cgaagtagcg
    29641 gaatgcccaa ttattgggat tacaggaacg aacggaaaaa caacgacaac aacgatgaca
    29701 ggactactac tgaatgcagg agcagatcaa ggaattgcac gtttagcggg taatattggg
    29761 tatccagcaa gtggtgtagc gcaagaagca aaatcagaag ataaaattgt gatggaattg
    29821 tctagcttcc aactcatggg aatcacagat ttccgccctc atatagcggt gattaccaat
    29881 atctatgaag cacatattga ttatcatggt acaagaaaag aatatgtcaa agctaaatgg
    29941 aaccttcaaa aaaacatgac agaaaaagat tatctgatcc ttaactggaa tcagtcagaa
    30001 ctacaagaat tagcgcaaag aacaaaagca cgcgtactgc ctttctcgac aaaagaagtg
    30061 ttggaagacg gtgtgtatgc tgacgattac tcaatatact ataaaaaaga aaaaataatg
    30121 gaaatatctg aacttggcgt accaggtaag cataatgtag aaaatgcact agctgctatt
    30181 tctgtagcaa aattgtatgg tatatcaaat gaagctatac gagaaacgtt gcattttttc
    30241 catggcgtac cacatcgtac gcaatatgta ggagaaatcc aaggcagaaa attttataat
    30301 gattcaaaag caaccaatat tttagcgact aaaatggctc taagtggatt tgaaacgtct
    30361 aaggtcgtgt tattggctgg cggattagac agaggaaata catttgatga attaattcct
    30421 tcattaaaag gaatcaaagc aatggtcgta tttggccaaa caaaagaaaa gttgatggat
    30481 gctggaaaaa aagcaggcat cgagacaata gtaacagccg attctgtaga acaagcagtt
    30541 cctttagcat tagaaaacag tacagatggt gatgtagttt tattatcacc agcgaatgcc
    30601 agctgggatc aatacccaaa ttttgaaact agaggaaaca gattcatgga agctgtgaac
    30661 cgattaaaat agaaaaagta gaagcgagcg ataaaaaatg aaaattttgg ttacaggcgg
    30721 aggaacaggg ggacatattt atccagctct tgcctttgtc aattatgtaa agactcaaga
    30781 accgaatgct gaattcatgt atgtgggagc aaaacgaggt ctagaaaaca aaatcgtccc
    30841 tgatacagga atgcctttcc ataccttgga gattcaagga tttaaacgaa aaatttccat
    30901 gcataacatc aagacggtcc gattgtttct taagagtatc cgtcaagcaa aaaaaatcct
    30961 cagtgatttt caaccagata tcgttatcgg tactggagga tatgtttccg gagcggtagt
    31021 gtacgctgca gccaaaatgg gtattccaac agttatccat gaacaaaaca gtgtaccagg
    31081 tgttaccaat aaatttttaa gccgatatgt ggatcggatc gctctttcat ttaaagatgc
    31141 ggcttccttt tttcctgaaa acaaagcagt tcttgtaggt aatcctcgag cacaagaagt
    31201 agcagatact aaaaaatcag atattttgaa aacatttgat ttggatccag aaaagaaaac
    31261 agtcttgatt tttggcggta gtcaaggagc acttaaaatc aatcaagcag tatcagtttt
    31321 tttaagtgaa tatattcctt cttcttacca agttttgtat gcttcaggag atagatatta
    31381 tgaagagatc ctaaataaaa taaatcctga aatacaaaat attagtgtcc agccttatat
    31441 caaaaatatg gcagaggtca tggcttccag tgatctgctg gtggggcgtg ctggtgcaac
    31501 ttcgatcgca gaattcacag ctctggggtt accggcagtg ttggttccca gcccatatgt
    31561 tacgaatgat catcagacta aaaatgcaat gagtcttgtt cacgcaggag cagcaaaaat
    31621 gattgcagat aatgaactaa caggtgaatc actaagtcag acagtcaacg aaatcatggg
    31681 tgatgaagaa ttgcaaaagc aaatgtgccg agcttctaaa gaacaaggaa tccctgatgc
    31741 ttcaaaacgt ctttatgact tagtaaaaca aataatcaaa aaataaaggc ggtggtctta
    31801 gtgagtaaga aaaaagaacc aaaaaaattg tctcatccgt cttctgatga aaacaatttg
    31861 actccttggc aaaaggctaa ccgaaaatat ctggcagaac aagagatgaa aaaccaagag
    31921 ccaaaaaaag aagacataac attagataca cccctttcag attctgaaga agaaaaaacg
    31981 gttcaagagg aacagcaaga agaaagccag tccgagaaaa aaatcgaatt ttttgaagag
    32041 atcaagcaaa agaaaaacgg tggaccatat aacggttctt ttttgaaccg gctgcccaat
    32101 ttaaaaagtc aacggcacaa agtactattt cgtcggttag ctttgatcat cactgtattg
    32161 ggtataccgc tgattttttt gatctattat gtttctcctt atagcaaatt acaagcagtt
    32221 gcagtatcag gcaataagac agtcaacagc caagaaatca tctctgatac aaaattgtca
    32281 ttaggagaaa atgtttgggg acagtatttt catcgatcta cttatatcga tcgccttaaa
    32341 aaagcacagc caagaatcga aacagcaaat atacatttca aagggatgaa tgaattcgat
    32401 ttggatgtaa cagaatataa agagattgct ttgatcgcaa aaaacaatca atactaccca
    32461 gtgattgaaa atggaacagt attagatgaa aaagtagcaa atcctacaaa aaatctccct
    32521 atcttggaag agttcaaaga ctctgcaaaa atcaaagaat taacaaagca atacaatcag
    32581 ttatcatcag aattacaaaa agcaatttct gaaatcaagt acacgccaag agcaagcaat
    32641 aaaaatctga tccaattgaa tatgaatgac ggcaatcaag ttattgtaaa tatcaataat
    32701 cttgcgaacc aaatgaaata ttattcgcaa gtagccaaag atatggatga aaaaggtgtg
    32761 atcgacatgg aagttggtat cttctcgcat ccttacggta gcactcaaga aacggaagaa
    32821 tcgggccaat cggaagaaac aactgaaagt gaagaaaatg cggatcaaac aaacgaatca
    32881 agtgaagagg cagaaacgca aaaagcacaa acacctggat cgtctagcac aaacggatca
    32941 tctgattcaa atgattcaag tgaaacaagt caaatagaag ataatccaag tacaactccg
    33001 tcaagtttaa atgaaaattg ataaaacaac tttaaaaagt aagaatgtac tttctaaaaa
    33061 ccagtaattt atggtagaat tggaagcagt gaatatatct attaataagt atattttaaa
    33121 acggacgcta gttgttcgct tgtatatgga atagtaagta ggagggaatc ccattcatgg
    33181 caaaaacagg aatgtatgta ggccttgata ttggaacgac atctgttaaa gttgtcgtgg
    33241 ctgaatatat cgacagccaa atgaatatta ttggagtagg aaacgcaaaa tcagaaggga
    33301 ttaaccgagg cattatcgtt gatatcgata aaacggtcca agcgatacaa cgagcagtac
    33361 gacaagcaga agaaaaagca ggtatccaga tcaaaggcgt aagtgtcggt ttgccagcaa
    33421 acatgcttga agtagaaaat tgccagggaa tgattgcagt taacggagat tcaaaggaaa
    33481 tcaccgatga agatgtcaga aatgtggctt cagctgccct tgttcgttca atacctcctg
    33541 aacgtcaaat cgtgtcaata ttgccacaag attttactgt cgatgggttc gagggaatca
    33601 aagatcctcg tggaatgatc ggcgtaagat tagaaatgta cggtctgttg tttacaggac
    33661 ctaaaacgat catccataac atacgtaaat gcgtggaaaa tgccggatta atcgtcaacg
    33721 aaatggtgat tactccttta gcgctgactg aatcaattct ttctgacgga gaaaaagatt
    33781 ttggtacgat cgtcattgat atgggtggcg gccaaacaac aacggctgtt atgcatgata
    33841 agcaattgaa gtttaccaat cttgaccaag aaggtggcga atttgtcact aaagatatct
    33901 ctattgtatt aaatacgtca tttaacaatg cagaagcgtt gaaaataaac tacggggatg
    33961 catacccaga acggacatca ccagatgaag aatttccagt agatgtgatt ggtcagtcag
    34021 aaccagtgaa agtcgatgaa cgctacttgt cagaaattat ttcagctcga atggaacaga
    34081 ttttcaacaa agcaaaagat gcattggacc aaatcgaagc attagagttg ccaggtggcg
    34141 tcgtattgac aggcggagct gctagtcttc ctggtgttgt ggacttggca caagaaatct
    34201 tcggcgtaaa tgtaaaattg tatgttccaa atcacatggg gctgagaaac ccggtattta
    34261 caaatgtaat aagtatcgtg gattattcag caaacttgag tgaagtgtat caattggcaa
    34321 aaattgctgt tacgggtgaa acctcagcag ctcgtcaaat ggtagttgaa caagaaacaa
    34381 caaacacata tgagagctat gaagcacctg aagaaacaat ctacgatgag ccggaaccaa
    34441 aagagtccgg agaaaatgta aaaaataaaa tcaagggttt ctttacaaat atttttgatt
    34501 aattcatcct gggaggaata agatatggaa ttctcaattg ataataatat caatgacggc
    34561 gcagtaatca aagtcatcgg agtcggtggt ggcggtggaa atgctgtaaa ccgaatgatt
    34621 gaagaaaacg ttaaaggtgt tgaattcatt acagccaaca cagacgtgca agcattaaaa
    34681 aattcaaaag ctgaaacagt tatccaatta ggccccaaat atactcgtgg tttaggtgct
    34741 ggttctcagc cagaagttgg acaaaaagcg gcagaagaaa gtgaacaatc cttacgcgaa
    34801 gctttagacg gtgcagatat gattttcatc actgcaggaa tgggtggagg aaccggtaca
    34861 ggtgctgcac caatcgttgc aggtatcgcg aaagaattgg gcgcattgac tgttggtgtc
    34921 gtgacacgtc cattcacttt tgaaggaccg aaacgtggcc gttttgctgc agaaggtatc
    34981 gctcgtttga aagaaaatgt ggatacatta ttaattatct caaataaccg tttattagaa
    35041 gttgtagata aaaagacacc gatgcttgaa gctttccgtg aagcagataa tgttttacga
    35101 caaggtgtac aaggtatctc tgatttgatc acagcgccag gttacgttaa ccttgacttt
    35161 gctgacgtga aaacagtgat ggaaaaccaa ggtacagcat tgatggggat cggtgtggct
    35221 agtggagaag atcgcgtagt agaagcaacg aaaaaagcaa tctcctctcc attgcttgaa
    35281 acatctattg atggagctga acaagtactt cttaacatca caggcggttt ggatatgaca
    35341 ttgttcgaag cacaagatgc ttcagatatc gtagcaaatg cagcaactgg cgatgtaaat
    35401 atcattctag gtacatctat caatgaagaa atgggcgatg aaatccgtgt gacggtcatc
    35461 gcaacaggta tcgatgaatc aaaaaaggaa cgcaagtcta gccgaccagc tagacaagca
    35521 caaatgcaat cacctgctca aaagactgta ttagatatgg atcaagctaa accaacttct
    35581 tcagaagaag aaaacagttt tggcgattgg gatatcagac gtgaacaaaa tgttcgtcct
    35641 agagttgatg attccaattt tgacaatatc gaaaaaaaag aatttgatac attcaaccgc
    35701 gaagaaacaa aatctcatgg cgatgatgaa ttaaatacgc caccgttttt ccgtcgtaaa
    35761 aaataaagga gaggaagagc acatgattgc tgacaacttg cagaaaatca agcaagaaat
    35821 tcaggattcg tgtgctcttg ttcatcgtga tccgaaagaa gttacattag tagctgtaac
    35881 aaaatctgtt gaatccgctc aagctgaaga attggttaga gctggcgtta tgaattgtgc
    35941 agaaaaccga gtagataaat tattagaaaa gaaacaagat ttatttgatt ttcctgaaat
    36001 aaagtggcat ttaattggta atttacagcg cagaaaggta aaattgattg taaatgaaat
    36061 tgattacttt catgcgttgg acagtttaag attagcagaa gaaatccaga agcggttaga
    36121 gaaagaatta tcctgtttta ttgaagtaaa tgtttctggt gaggaatcaa aacatggaat
    36181 ttcaccagat gagcttttac cgtttgtgga atctttagct gcctgcacaa aaatcaaggt
    36241 tgttgggttg atgactatgg cgcctaaaga agcttctgat gagcaaatcc ataaagtgtt
    36301 tggtacgctg aaagaattgc gtgatcttgt tcaagcaaaa ggttatgccc atgcaccatg
    36361 tagtgaatta agtatgggga tgagtcaaga ctttcaaatt gcgattgaag aaggtgcaac
    36421 atttatccgt gtaggaacag cgctattcaa agacgaataa aggaggaaca aaaatgccgc
    36481 ttttaaacag aggagcaatt gctaattttt tcggattagc tgatgaggaa gagtacgaat
    36541 accaagatta tcctgaaaca acaaaaaagg ctgtacagcc aactaaaaaa aatacaacac
    36601 agaatcaatc aactcattct gtatctgctg cgaatactca tgaaaaacaa aaaacggcga
    36661 cttcacgtcc tgtaacgcat gaaagtattc agccggctga aaagaaaaaa gtaattgaac
    36721 tacatcaatc acctacaaaa actgtagcga aagaaagaac agcttcaaga acctctacac
    36781 aacctaaagc aaatcttgct ggaaaaatca ctgtaatgga accaagagct tattctgaag
    36841 ctatggcgat tgctaaacga atcattgctg gagaagcaac cttagtgaat tttcatttag
    36901 tagaagaaaa acaagcgaga agaatcgttg attttctaac aggaacagtt tatgcactag
    36961 atggagatat caaacgtgtt ggagatgaga tttttctttg tacaccgact gggatggaga
    37021 ttgacagttc aaccgctcaa tcttttgcag atgcgaattt gtttgatctg taagaggagg
    37081 aactacgatt tatactattg cactattact tcatttactt caacgggcag cctatttcta
    37141 tagcattatc ttgatggtat atgcattgct ctcttggttt ccaggcgggt atcaatcagg
    37201 gctaggacga ttattagcta agatctgcga accgtatatc agtttgttcg atcgtctgcc
    37261 tttgaggatt ggacccttgg atttcacaat tttaggggca atcctgatct tgaacttagc
    37321 cgtacaaggt ttagcacaga tcgttatatg gataactgtc catttagttt attgattgaa
    37381 gcaacaaaat ctttattctc agaatagata ggaggggaaa attttggatg caaatgtata
    37441 ccagcacttt cgggcagatg aacgtacatt tatcgattcg gtaggcgatt ggattgaaca
    37501 ggttcaagga caatatgcac cctatctgac cgaatttctt gacccaagac aatcctatat
    37561 tttagaaaca ctgattcgac aaagcagtga tttgcgtttc atgttttatg gcggatatga
    37621 acaggcagaa agaaaaagat gtttgatttt ccccgattat tacgaacctg tagaggaaga
    37681 ttttgaaaca gcattgtatg aaatccatta tcctagcaaa tttgccagcc taagtcatgg
    37741 gaaaatcctt ggaactttag tgggaacggg gatcaaacgc tcttactttg gcgatatcat
    37801 ctcggacggt gatcgctggc aagtatttat tgcaaatgag gtggatcatt ttgttgctgc
    37861 acaagtaaca aagattggaa aggttgctgt acgcttggaa ccaatcaaat acacagaaat
    37921 gattatgccg aaagatagct ggacacaaga aagaggaaca gttagttcca tgcgactaga
    37981 cagtgtgatc tctgagattt ataatatttc cagacagcgg tccaagcagc tgatcgaagc
    38041 tggtaaagta aaagtgaact ggacagaaaa tacaagacct gattttgtac ttgaactatt
    38101 agatattatt tcagtcagag gttatggcag agtccagatc caagaacttg aggggaagac
    38161 gaaaaaagaa aagtacaggg tactgttcgg tgtattacgt aaataaaata aaaaggtggt
    38221 ttactcatgg ctttaactcc attagatatc caaaataaga cattcccgac aaaaatgcgg
    38281 ggatataatc aagacgaagt agatgatttc ttagatttag ttgttcgtga ctatgaggaa
    38341 ttgactcaaa gaaatcgaga attagaaaaa gcagtcaaac attctgaaga aaaacttgag
    38401 tacttcaatg aattaaaaga tgctttgaac caatcaatca tcgtagctca agatacggct
    38461 gataaagtaa aaacaagtgc aagcaaggaa tccgaagtga tcgttacttc tgcacaaaac
    38521 aaagcagatg agttagttgc gaacgctgaa aaacgtgcac atcaattaac aactgatgca
    38581 gaagaaaaag caagaaaaat cttgacagat gcaacagaaa aagcaagaca gcttgcgaca
    38641 gaaactgaag atcttaagaa aaaaacacga gtattccatc aacgtatcag cttgatgctt
    38701 gagtctcaat tagaacaagt gaaaagtccg gaatgggatg aaattttaca accattctcc
    38761 agctacgtga ctgacagcca tgaagtgatc aaagaagttt tagcaaaaca gcttgacaat
    38821 gaaaatgata ctgatgtaaa ctcagacaca ggagtcatag aagcacctat gacggcattt
    38881 gatacacagg ctattgatat ctcgatcgtg cctggtgaaa atgaaaacga gtagaacaga
    38941 aaagcacttt gttattctta aagcgagccg atgagagtgg aagttcggta agacactgca
    39001 tggtgaaaga tcctctactt gagacctgct tttccgaaat tagagtaaga agcagcggct
    39061 gacctcgtta ttggtcacag agagtttgca ctagatagat gcaactaaat ttgggtggta
    39121 ccacggcatg ttcgtccctt gcggctgaac atgctttttt gtgtccggaa aacaaataaa
    39181 ggagtgtatg gttgaatgaa aatgaaagaa accttgcaat taggaaaaac aaaattctct
    39241 atgcgtggta atttgccaaa ccgagaaggc gagtggcaaa aagaatggga agaaaatcga
    39301 atctaccaaa aaagacaaga gttgaacgaa ggaaaaccta gctttgttct tcatgatgga
    39361 cctccatatg ccaatggtaa tatccattta ggacattcat tgaataaaat cagtaaagac
    39421 attatcattc gttcaaaatc aatgtctggc tatcgtgcac catatgttcc aggatgggat
    39481 acacatggat tgccgattga acaagtattg gctaataaag gcgtgaaacg taaagaaatg
    39541 tccatggcag aatacttgga aaaatgtcgt gaatatgcac tttcccaagt agataagcaa
    39601 cgagcagatt tcaaacgcct aggagttgca ggagattggg aacatcctta tgttacatta
    39661 gatccttctt atgaagctgc acaagtacgc gtatttggta aaatggctga aaaaggctat
    39721 atttacaaag gattgaaacc aatttactgg tctccgtcaa gtgagtcttc tttagcggaa
    39781 gctgaaattg aatataaaga tgtaaaatct ccatctattt ttgtagcttt caatgtcaaa
    39841 gacggcaaag atattcttga tgaagataca gcttttgtca tttggacgac aacaccatgg
    39901 acattgccag caaaccaagg tattgctgta aatccaaaat ttacgtatgt attggtggaa
    39961 gccgatggcc gtaagtttgt cgtagcgaaa gatttgctag aaacagtcca atcagaaatc
    40021 ggttgggaaa atgtaacagt tctaaaagaa tttgctggac aagaaatgga atatatgact
    40081 gcaagtcatc cattctatga acgtgaatca cttgtgattt taggcgatca tgttacttta
    40141 gacgctggta ctggattagt ccatactgct ccaggacatg gggaagatga ctatatcgct
    40201 ggtagcaaat ataaactatc tgtagtgtca cctgtagact caaaaggatt gtttacagaa
    40261 gaagcacctg gatttgaagg tatcttttat gacaaagcaa atcctatgat cactgatcta
    40321 ttaaaagaaa aaggagcttt gctgaagcta gatttcttta ctcatagtta tcctcacgat
    40381 tggcggacga aaaagcctgt gatctatcgg gcaactccac aatggtttgc atctatcgat
    40441 aaattccgtc agaatatttt ggatgaagta gaaaaagtag agtgggttat cccatggggt
    40501 aaaacacgtt tgtataatat gatccgtgac cgcggcgatt gggtaatttc ccgtcagcgt
    40561 gcatggggag taccacttcc gattttctat gctgaaaatg gagaagcaat catcacacca
    40621 gaaacaatcg aacatgtggc aaaattattt gctgaacatg gatcaattat ctggtttgaa
    40681 aaagaagcga aagaacttct tcctgaagga tttacccacc caggttctcc taatggcgaa
    40741 tttacaaaag aaaaagatat catggatgtc tggtttgatt ctggttcttc tcatgaagct
    40801 gttttacgtc agcgaccaga attatcattc ccagcagata tgtatttaga aggatcagac
    40861 caatatcgtg gctggttcaa ctcaagtatc acgactagtg tggctatcaa tggtgtagca
    40921 ccatacaaat cagttatttc tcaaggattt gctttagatg gtgaaggccg taagatgagt
    40981 aaatctttag gaaacatcat tgcaccagaa aaagtgatca aacaatttgg tgcagatatc
    41041 cttcgcttat gggtatctag tgtagattat gaagcagatg ttcgtgtttc tatggatatc
    41101 ttaagtcaag tggcagaagt atatcgtaaa atcagaaata cgatgcgttt cttattagcg
    41161 aatacagaag atttcgatcc taaaaaagat actgtttcgt acaatgattt acgttctgta
    41221 gacaaatata tgaccgttcg attgaaccaa gtaatcaaag aaatccgaga agaagggtac
    41281 gacaaatata acttcatgca tatttaccgt acagttatga acttcttgac ggttgatcta
    41341 tcgtcattct acctggattt tgctaaagat gtagtttata ttgaagcaga agatgattat
    41401 caacgtcgtt gcatgcaaac tgttttctat caaacagctg tagctttgac aaaattattg
    41461 actcctatca ttccgcatac agcagaagaa atctggtcat tcctgaaaga agaagaggag
    41521 tacgttcagt taagcgagtt tcctggttat gaagagtttg caaaccaaga agaattgatg
    41581 gatacctgga ctgctttcat ggacttccgt gataatgtct taaaagcatt ggaagaagca
    41641 agaaacagca aattgattgg taaatctttg gaagcaaaac taactgttta tccaaaacaa
    41701 caagttcgtg aaatgttaaa agctttggat gcagatattg cacaattatt gatcgtttca
    41761 ccagactact ttgaagttat ggcagcagat gaacaagtgc cagataacgc tatggtattt
    41821 gatgatgttg ctattacagt ggaaaaagca gacggggaaa cttgcgatcg ctgtcgccaa
    41881 gttcgtaaag atgtaggagt agatgaaaaa cttcctcatc tatgcggtcg ttgtgcaaaa
    41941 atcgttgaag acttctatcc tgaagctgta gctgaaggat tcgaagaaaa gtaaaaggga
    42001 aaagggggtg tgacatgttc tttgtcacac ccccttttgt tatcaatcgt ttaattttcc
    42061 acgttcacga taccagttat ctggttgcca gatccattcg aatccgtctt tttcaagtag
    42121 ttcgaaagct gctttaggtc ccattgatcc agctttgtag ttgggaaaat ccggttgtgt
    42181 ttggtcccaa gcttttcgaa tgacatccac gatatgccaa gattgagcga cttcttccca
    42241 atgagagaag ttcgttccat caccattcaa cgcatctaat agtaattttt cgtaagcttc
    42301 tgggctattt tcaaccattt ctgcactatt tctaaattcc aatttgattg gttccgtttc
    42361 aaatccttgc cctacttctt tcccattcaa ggataaagaa aatccttcag ttggctgaat
    42421 atagatcgtt agaacattag gtggaagtgt cgtatcgtca catggttcat cgacagatgt
    42481 tttgaataca ttaacaggaa cttgtttgaa tacgatgttg atccgagtcc ctttttcagt
    42541 caaacgtttg cctgtacgta catagaaagg aacacctgac caacggaagt tatcgatagt
    42601 gaatttgcct gcgacgaatg tttcagtctc tgattgagca tccactttgt cttcttcgcg
    42661 atagcccttg aattgtacac catccaattc accttgagca tattgcccgc gaacgaagtt
    42721 ttctaacact tcttcttcgt tataaatgcg gatagctttc aatgctttga tcttttctgt
    42781 acggatttct ttttcggaaa atgctactgg cggttccatt gcaagtaatg ctacaacctg
    42841 taaaatatga ttttgcacca tgtctttaag tgcgccacta tgatcgtagt agcccccgcg
    42901 atcttctaca ccgaggtttt cgccaaaagt aatttggata ttgtcgatat agcgattgtt
    42961 ccattgagcc tcaaaaatat tattggcaaa acggatagct gagatgtttt ggatcatttc
    43021 ttttcctaaa taatgatcaa tcctgaaaat atcttgttct gggaatactt ctctgatttg
    43081 ctggttcaat tcaaaagcag actggtaatc agaaccgaac ggtttttcta tgatcaaacg
    43141 attgaagcca ttttttgtca taatattttg tgatttcaaa tgagaaacga tcgttccaaa
    43201 aaattgaggt gccatagcca aataatagac acggttaccg tttaattgat agtgtccatc
    43261 taatcggtca gacaattctt ttaatgtctg ataatgcgtt gaatcattta catcatggga
    43321 ttgatagtaa aaatggcttg caaaggtagt tgcttcttca tctgaaggat tcaatgcacg
    43381 gattgtttct ttgacaactt cacggtagtg ctcatcgctc cagggacggc gagcggttcc
    43441 gattacagca aaatgctcgc ctaaatttcc ttttctgtaa aggcggaaca aagacgggta
    43501 caatttacgc tgggctaggt cacctgtacc gccgaatatc gtaaacaata catttttttc
    43561 attagacatg tggatacact cctttatatt ttatcgacaa aaatcgtgct ggccgcctta
    43621 aagctgacgg ggattttctt tctatcattt tggatagtga tcggtccttc ataagctgcg
    43681 atgtcaacga tctcatattc ttcgttgata ttcaggtcaa tagtgactaa gtaatccaac
    43741 aattcttttt catcaagcac tcgtgcgata cgcacttttg taccgacagg ataggactcc
    43801 aatgtttgtc ggcgttcttc atggacaact tcctcttttt taggaatcga tcctccatga
    43861 ggacaatgtt caggatgatt caaatagttt tctaaatgat ctgcaagatg ttccgaagtc
    43921 acatgttcca atacttcagc atcatcatgg acttcattcc agctatagtc taaatgttca
    43981 actaaaaaaa cttcccataa gcgatgtttg cgaatcagac tgctagcttt ttgcagtccg
    44041 cttgcagtca attgtacacc ttgataaggg gaatgttcta caagctcttc tttcacaagt
    44101 ttagaaatca tttcactgac agatgcagct gagacatcta gactagatac gatttgttta
    44161 ttgttgactt tcgttgtatc tccacctagt tctaaaatga ttttcaaata atcttctcga
    44221 tttggtgtca tattcgatca tcctttcatg gtaaaaaacc atcattagta agtgtaacaa
    44281 aaaaaacgtt aattgactat ttttctaaat aaaaaacaaa ggatttttag acgtttttac
    44341 aacaaaaaac gaaagatgat agagaagact cattctttcg tttataaagg atatatatat
    44401 acattgggag ggatctattt ttcagataac aacttatatg aagtgtctgc agattcgtca
    44461 taaagagttt gttcgggata agaaaagata agataaaaac catactgatc acgtacgact
    44521 aaacgatatt catctatttc taccacagat gtgccaagag actgaccgtt gatcaaaaac
    44581 gtgtaatcag atgtaatctg taattgttgt ttttgctggc tatcactaaa gaaccatgta
    44641 ccttgatact ttgagagtcc ttctgacttt tccgcttgtt ttttcttttt caagtaaata
    44701 aaacttgaga gaacggtaat gagtgaacca agaaaataga tcattttttt tatcaccaaa
    44761 tcactccttt atgagaaaat aatcgcatag acatattctt tcttctttat ttcattgtac
    44821 cgaataaaag ctaaaatttc tctaagactt tcgactggtt tttttactgt ttttctacaa
    44881 attatgatag gatacatagt aggaagaaag gtgacataat ttgaagtatc agcaagtgga
    44941 aatagctttt tttattgacc gcccgaatcg gtttatagca cattgtatga aaaaagacgg
    45001 agaaataatt attgcgcatg tcaaaaatac aggtagagga aaagaggtcc tgataccagg
    45061 cgctgaagtg gctattgtat ttgctcctgg acctaaacgg aaaaccgcct atgatctgat
    45121 tgctgtaaaa aagaagggtg actggtttaa tatcgatagt caattgccta atcggctggc
    45181 aattgatggt attttagacg gcacgatcct tttaccagga ttaactggtc catttacggt
    45241 atacaagcgt gaagtaactt atcttcactc caaatttgat atttatttag agacggtggt
    45301 cggacaaaaa gcatttgttg aagtcaaagg tatgacactt gaaaataaag cgattggtgc
    45361 ttttcctgat gcgcccacac tcagaggatt gaaacatata ggagaattaa cgtatgccgc
    45421 acaagatggt tatgccgctt atgtcttgtt tattgctcag ttcgaacatc ttcatctggc
    45481 aacgatccat gaagaaatgc aaccagcact agctgatatg gttcgacatg cgcagcaaag
    45541 cggagttcaa atcctcgcct ataattgtca agtatcggca gatgaggtaa ctgtcaagca
    45601 ggccattcca tttgatgtag atatcccttt tgaagatcct atacaataga aagaaggtaa
    45661 aataatgatc aacttaggta ttatcggaac aaattggatt actcatcaat ttgtccaagc
    45721 agctttaagc acaaaaaaat atgaattgac agcggtatat tctagacatt tggaaaaagc
    45781 acaggaattt ggttctcatt atgaagggga tatcgaattc gcaacagatt tgaagacttt
    45841 tttcaatatt actcatatgg acaccattta tattgcttct ccgaatagtc ttcactttag
    45901 tcaagctaag caagcaattt tggcaggaaa aaatgtgata gttgaaaagc cagctttttc
    45961 gacgccaaaa gaaatggatg aaatcatcca tttggcaaat gaacacaatg tgtatttttt
    46021 tgaagcagca agaaatatcc atgaatcagg ttttaaaaag attgccgatt acttaccatt
    46081 aaaagacgag ataatcggtg caaatttcag ctatatgaaa tattcttcgc gctatgacca
    46141 agtattagat ggagaagaac cgaatatctt ctcgactcat ttctcaggtg gtgcattgat
    46201 ggatttaggt gtttaccttg tatatgcagc tgttgcttgg tttggaatgc caaaagaagt
    46261 ccattatttc ccaagaaaga tttctactgg tgtagatggt cttggatggg ggatcttacg
    46321 ttatgagaat tttgatgtat cgatccagcc aggtaaaatc ggtgattctt atttaccgtc
    46381 agagatttat tttgactcag gaacgctagt gatgaatgga gtcaatgcaa ttgaacaagc
    46441 acaatttcat gatcgcgcgc ataaagaaat cattgatttg gcggtaacag ctgcagaaaa
    46501 tccaatggca gatgaagcag aggattttgc caatgtcatg gctcatccga aagatccagc
    46561 ttggggattg ttgtatgaag agtggatcga gctagctaga aatgtgaacc aagtgattta
    46621 tgatttaaga aaaaatggcg gcatcaaatt cgatgcagat aatgaggaag atttttaatg
    46681 aacgaatttg tacaagtatt tcccgaaaat tggcagaaaa aatatgagga taaagggttt
    46741 atctcacctt ctcttattca acgagcagtt tttcagccac taagtaataa acaaagtata
    46801 gtagcggttt ctcctacagg ttcaggtaaa acactggctt atctttggcc tctactacta
    46861 aacgtggaac caggtgaagc aagtgcgttg gtcatttttg cttcttctca agaactagct
    46921 attcaagtag cagatgttgc acgtgaatgg ggaaaagaca aagagttgaa agtccaatct
    46981 ttggtgggcg gagccaacgt caaacgtcag attgagggtt tgaaggaaaa accagaaatc
    47041 ttgattggta caccaggaag aatattggaa ttgatgaaag caaaaaaaat aaaagcacat
    47101 caagtgaaaa caatggtatt tgatgaagca gaccagctat ttgacgctgg gaacagccaa
    47161 atcattgatc aaattcttca tcaagctcca acagagtatc agctagcttt tttttcagcg
    47221 acagcagatc gttcccttga aagcattgaa aaaatcactg ggaaaacgtt tgagacaatt
    47281 gacgtgacag cagaggacga ttctcgaaaa ggactgagac attacttttt acgtgtaccg
    47341 atacgaaaga aagatgacta cttgagacgt ctcacgcata tagaagattt tcatggtctg
    47401 attttcttta atcaactgac tgaattaggc gtaatggaag aaaaattgct gtatagaggt
    47461 gtaccggtag gaagtctggc ctctgatcag aacaagctac tccgaaaagc agcgctagaa
    47521 caattcaaaa agaaaatggt cagagcactt ttgaccacag atgtggcggc tagaggttta
    47581 gatattacag gtcttcctta tgtagtgaat gcagaagttc cgttaagtga agaagcttat
    47641 ttgcatcgtt cgggaagaac tggtcgaatg ggtaatgaag gaatagtaat tacactcgta
    47701 caagataata atctaagaga tttaaaacgg cttttacgtc catcaaatat aacacttgaa
    47761 gagatttttt tatacggagg taagctgcaa actgagcagc caaccaaaga agatgcagct
    47821 cctgcagtaa ataaaaagta tgcgaaaact ttttctggaa aggagcagaa cccagaatat
    47881 aacaaagaaa aaataagaaa aaaagttaaa aataaatcaa aaaaagagag aaataaagga
    47941 gcaaggcgca aataagcgcc tttttatgct atgattatga aaacgggaac aaaattggtt
    48001 gccagaggag gggttatgtg aaagaacgtc aagaagagat cttgcagcta ttgacagaaa
    48061 accaacaagg attgacagca agtgatgtag ctgagcgctt aactatcgat cgaagcaatg
    48121 caagccgata tttaagtgaa ctatacaagg cccatcatat tgtaaaaacc gctggccgac
    48181 cagtcgttta ctctttgcca acggaaaagt caaaatctga tgaagtgcat gtcgactctt
    48241 ctacgcaagt gacttttgaa acactcgttg gagaaaatga ttctttaaaa gtcagcatac
    48301 agcaggcaaa agctgcgatt ctttatccac cgcgggggtt gcatacgatt atttttggag
    48361 aaactggtac agggaagtcg atgtttgctg aatgtatgta tcattttgca atcgattcag
    48421 agatgctttc tgcagatgcg ccatttgttt ctttcaattg tgcggattat gctcaaaatc
    48481 cgcaactgct ctttggacac atctttggga tcaaaaaagg ggcttacaca ggagcagctc
    48541 aagatagtcc cggtctaata gccaaagcag acggcggtat attgttttta gatgagatcc
    48601 accgtttacc gccggaaggg caggaaatgc tgtttacttt cattgataaa ggtatttacc
    48661 gtccgcttgg agaaagtgca caagtacatg aagcatctgt tcagatcatt ggggcgacga
    48721 ctgaatcttc agaaagtttc ttatctacgt tcaatcgacg gattcctatg gcaatcacct
    48781 tgccgaatct tgcttcacgt tcactggatg agcgttatga aatcatctct ctttttatca
    48841 aacaagaagc gaatcgtttg aatcaacgta tcgatatcga aaaagaagcg attcttgcct
    48901 tcatgcttta tgatgcggaa ggaaatatcg gtcaagtgaa acgagacttg aaactagtct
    48961 gtgcaaaatc ttttttacat taccgtactc atcatgagaa aaagctaatc attcgaaaag
    49021 aagaactttc ccttcaagta caaaaaggat tactgaaaat caaggaaata ccggaacgtc
    49081 tcgaccgatt catggacagc aaaagtcagt atttgacatt tgaaccagga tttgctgatg
    49141 ttgtctggtc acaagatcca gaacgaaaca tgcaggtcta taacgatatt gaggaaaaag
    49201 tcacttctct ttccgaaacc ggtgtggaga acattgatct agagaccctc atttcaaaag
    49261 atgtggatgc ttatttccag acatatgtca aagaattgac taaaagttcg attcccaaag
    49321 atcttttgcc agatgaaatc tggcggttaa ccaatcaatt gtatgatgtg gctgaaaaac
    49381 ggttagaccg tatttataat gaaaaagctc gttttgcttt tgctttgcat ctgcaaagta
    49441 cattggatcg tgtgagagaa ggtcatatga tcgtccaccc agatttgaat aacgtacgga
    49501 aaaattttaa gagggaattc caggtagcaa tcgatctttc aacgatcatt gaagaagaag
    49561 aacaagttga gattcctttt gatgaaatcg gctttattag tatgtttctt tctatcaatc
    49621 taggagaaag tgagcctgtt aaagaaaaca aagtagatgt tctcatattg atgcacggca
    49681 gacagactgc ttcaagcatg ttggagatgg cacaagaact attaggaaca aaagtaggga
    49741 aagcttttaa tatgccactg gttatggaag ttcaaacaat gtatgaagaa gtgttacaat
    49801 atgtcaaaaa ctatcaagag catttgacga atggcctact cttgttaaca gatatgggat
    49861 cattaaacac cttcagtgat ttgatttatg aagagactgg cattcggacg aaagctatca
    49921 cgatgacaag taccatgatt gtgatagaag cgatacggat ggcaagtgtt ggaagaagct
    49981 tagaagatat ttatcaaaat atccagcttt cctttgaatc gatcgtacgt gatcagttca
    50041 aagcggaaaa gtctgaaact ttacagcgta aaaaagccgt aatcgtgaca tgctttactg
    50101 gtgaaggagt agctgccaaa ttatatcagc gtattatgcc agtgattaat caaaataagg
    50161 tagaactgat ccagatgcag tttttagaac gtgaatcatt caaaaaacac atcgatgcct
    50221 tattggaaga atatgagatc aaagcaattg cgggaacagt tgatgttgat tatcaaaata
    50281 tcccattttt ttcagcctat gacgtttttg atgatgaaaa actaaatatt ttaaagcgta
    50341 tagctgatga tgagatcccg acagctaaaa ttgtcgattc acttgctgga tcattaagcg
    50401 atgtcttttc tattgatgaa ctgatccgtt cattgcaaaa gacagttcag cagatccaga
    50461 ccgatttgtc gattatcgtg gaacctggag tagatactgg aatcgttatc catttagcct
    50521 tcatgttaga cggattgatc aaacaagaaa gccatcggca atttgagaaa ctgtctattt
    50581 tctcaaaaat ttatcgtttg gaaatggata tcgttcgggc aaacttgatg gcactagaac
    50641 gaaaatatca ggtaaaaatt ccagaagatg atatcgcatt tcttacacaa atgtttcttg
    50701 aaaataaaat aaaaaaatga acacactaat catactgttt acacactaaa catctgtgtg
    50761 cgcactataa aaaagagcta aaaagcatga aaacgctatt tcagctcttt tttttattgg
    50821 catgcttctt gcgttatata aaagtgtaaa aaaataaaag gagtgttaaa aatggcaaaa
    50881 aaaacaatca tgttagtttg ttcagcaggg atgagtacga gtttattggt cacaaaaatg
    50941 caaaaagcag cagaagaaaa agggatggaa gccgatattt ttgcggtctc tgcctcagat
    51001 gcagataata atttagaatc taagaatgtc gatgtattat tattaggtcc tcaagtacgt
    51061 ttcatgaaag ctcaatttga acaaaaactt gcgcctaaag gtattccatt agatgtcatc
    51121 aatatgcaag actatggcat gatgaacgga gaaaaagttc ttgcacaagc agaaaatcta
    51181 atgaaataaa tcaagtatgc agttgggggt ataacaacgt ggaagatcaa caaaatctag
    51241 aagccatcat ggggttaatc atgtttggcg gtaatgcaaa gagcgatgca atggaagcga
    51301 ttgcggctgc taaaatcggt gattttgagt tagcggatag gaaaatcgct gatgcagaag
    51361 aatcacttat acaagcgcat catgcacaaa cagacatgtt gacacaagaa gcacaaggaa
    51421 atcatatgca agtgacatta ttgactgttc acagtcaaga tcacttaatg acatctattg
    51481 cgtttactga tttagcaaaa gaaatcatcg aactttatcg tcgcgtatac agcgaataaa
    51541 tacaacttct tttttgaacg gaagaagaaa aatttttgaa cggatttttt attttgttat
    51601 aaggagaact ctctttatat aaaaaactta taggaggaag taaaaaatga acggactatc
    51661 aagttggatg gagaaataca tcctgccggt tgccgcaaaa atcggagcgc aaaaacattt
    51721 agttgctttg cgtgatgctt ttatcggcat actgcccgca acaatggcgg gatcagtcgc
    51781 tgtaatgatc aatgcaatta ttcgtgattt accgcaacag tttaacagta cctatgctga
    51841 cagtttagca gcagacaaag gaatctttgc cgtattgaat gtcatcatcg gaatcaatgg
    51901 ttttgtctgg aacggtacac tagcaatcgc cggattgatc tttgcctttt cttgggggta
    51961 taatcttgcc cgagcttatg gagtaaatga tctagctggg gggatcgttg gtttagctac
    52021 cttgattcaa ggtatttcct ttggtccagc attatcagca gcattagatg tgaatgttcc
    52081 ttcaaatgtc gcagatgcaa tcaatagttc tgagattggt gcaacgattg cagatggcac
    52141 actgtcagtt ggattatggg gttggctaaa actagatcat ttgaatggaa acgcttattt
    52201 tacagttatg atcatgggag ctatttcagt cattattttt tgtaaattga tgcttgccaa
    52261 cattacgatc aaaatgcctg attcagtacc accagcagta tctaaagcat ttgcagccat
    52321 tttaccagcg acgatcgctt tatacgtcat tgctattatc aattatatcg taggtcgtgt
    52381 agctgatggg caactgatca ttgatttggt gcaaaaatat attgcagaac cattcctagg
    52441 attatctcaa gggttgggag cagtattgat cgtcacagta tttgttcaag tattctggtt
    52501 ctttggtatt catggatcaa atgtattggc accagtgcta gagggaatct ggggacaagc
    52561 acaactaatc aatattgata ttttccaaaa aggttataac ggaaaaactg gtacaccagc
    52621 cgtgttagat gcaatcgaag atggaaaagc ttatatgtgg gtccgcggat cgttcgatgc
    52681 ttttgcatgg tttggcggat caggcggaac gatcgtctta ctgatagcta tcttgatttt
    52741 ctctaaacgt gcagattatc taacagtagc taaattatcc attgggcctg gtatcttcaa
    52801 tatcaacgaa ccagtgatgt ttggtctgcc aatcgtattg aatgcaatca tgtttatccc
    52861 attcttgatt gctccagtag tagcaacagc aattggatgg acagctactt atcttggcct
    52921 agtcgctccg gtatcgcagc aagtcacatg ggtagtaccg ccgttcttat tgtcattctt
    52981 agcaacagga gcggattggc gggcaccgat cgtaacatta gtatgtatgg tcgtgacttt
    53041 cttgatttgg gcaccatttg tcatctctgc aaacaaaatg gaccctgccg caggcgaaca
    53101 acaataagat gaacgtgagg agcgggacgc tttaagtttc gttcctcata atgaattttc
    53161 gaggaggaac tgaaatgatt acaattgcca gtgcaatgca acaagatgaa atcaaagaat
    53221 tgttagaagg atacaatgaa gaaggaatga cttttacatt taaagaaaaa caaggaatca
    53281 agctgttctt tgaaacaagt gcagaagatc cggaagttgc ggctaaaaaa gcaaaggaac
    53341 taatcaaagc acagccttgg ggaacagtcc tttattttca atcaacagct gtttagaaaa
    53401 tggtttaaaa agataggatg gttgagatga taaaaaacgg tttattttta tgtagtattg
    53461 gttctttgat cgtgttgtat agtttgaatc cacattcgca aaggtatgat ttgtatagta
    53521 tggtcacagg tctttttcta acaggattag gaatctattt cttcatgaag gggaaaaaaa
    53581 gagaagaagc aaaggagaaa aataactaat ggaaatcacg aaaaaattaa atattccagc
    53641 atcgtttttt tacgataaag ttatggactc tgtccttttc gatgtacgta aagcgaccgg
    53701 caaatcagta actagaaaac agctgaaaaa tttggagtac gtcaaacaat tctcgcaaaa
    53761 taaccgggct cgcataaaaa tcgaagaagt cattgaaaac cagtcttata agtttcgtac
    53821 atcgacaacg aagaatgaat tcgtcgttca ttatcaaatc aaaccattgg acgattcgac
    53881 ctgtgaggtt cattacacag aaaaaatgga gtcttatggc atgcttcaaa agttgaatga
    53941 tatgctttta ggaacaatct tgatgtattt taaacggaga cgtttcaaaa aaatgctaga
    54001 tatgatcgaa gcaacgtatt aaatcaatga aacatggtac tatttagtta ttgaaaaatg
    54061 aagtaagcta gaaggaattt ttcataggaa gatagatctt tcctgcgaat cctcactagc
    54121 ttttttgtag gaaggggcag caaaatgaca aacaaagtaa gagagctagg tgtatcttcg
    54181 attgaggaaa tgtttgattt agccacttat gcatttaatg gggcagatac acctgaaagg
    54241 cgagaacgtt ttcgtacact tgcggagaat tcttggaatt acggattttt tgatgaggag
    54301 gatcggctaa caagtcaagt aatggcgacc ccttttccag tgaattttta cggacaagag
    54361 tacctaatgg ccggcattgg ctatgttgct tcttatccag aagcaagggg acaaggcggg
    54421 atcaatcaga tcatggaaaa gatccttgaa gattgccgtg atcgtaaagt aagcttatct
    54481 tatctggcgc cattctccta tcccttctat cggcgctatg gctatgaaca atcatttgat
    54541 aaaatcagtt atcaactaaa cagtcgtgat gtgccgttta tcaaaaaaaa atctggaaaa
    54601 ataaagcgta tggattttga agatgcaaaa gctgcaatca gaagaattta tgaacagatg
    54661 ccggaaaacc aacgagctgg tctgaagcgc gaagactggt ggtatacgta taaattcaag
    54721 caaaaaggca attaccagtt tgctttgcat tttgatgata atgaggaagc aacgggatat
    54781 atcgtttatc agcttgcaac gtcttccttt attattgcgg agtggggatt tttggatcat
    54841 gatgcttttt gcagccttgt tcgcttcgta agttctcaca atggtgcttt tgaaacattt
    54901 gaatattcat gcggctacgg cggtaatgat caaaattatc tattagaaaa tccatttgct
    54961 tctgtaatga tcacacctta tatgatggcg aggatcgtag atatttcttt gtttttggaa
    55021 aattatccat ttaaaaagag acaagctgca tttgccctac aaatagaaga agatcattat
    55081 gcgaaatgga atgaaggaat cgtggaagta acaatcgaaa atggaaaaaa tcatctctca
    55141 aaagtagaac aaacaaaact cccaatcgtt cgaggaagta tccaacatat cacgcagcta
    55201 ttattagggt atcgtaaagt ggaagagttg gtattccaag aaagaataca ggtgacaaaa
    55261 gaatatgaag aaacgttaag ctcaatcttg ccacagcaac agccaattct taatgactat
    55321 ttttgataaa aaacgggtga tgacaatatt tttgtcacca cccttttgtt tttcatttgg
    55381 catttgaagg aaaatgttaa agtaaatcct cttttttcgt aaatttgaat tttttgtttt
    55441 cttttgtttt agggttttct tttttgtggt cttggattgc ttctttcgta atcttttccc
    55501 gatttttttt agatgaatct aagcgttctg cgtatacttt ttcttttggc ttatcattca
    55561 ttggaaagga cctccctaca ttatttcatt ctacctctag tttatcatct gttaaggact
    55621 agtggaaacg ttttgcctaa cttataagct gattttttta gcagataagc ttgcaagtaa
    55681 aaaacaaaca agctacaatt gatttattga aattgttcct gtagctcagt aggatagagc
    55741 aatcgcctcc taagcgatag gtcgctggtt caattccagt caggaacgta taaataaccc
    55801 ctgtatcctt tgtggtacag gggtttgttt tgtatttgtg ccaaaaatgt gccaaactag
    55861 ttatcatttt tatataaact atcgaataca gcattgattt tttctttatc ctgatcttca
    55921 agttctcgaa cgatatgaag ataagtagac attgtagttt ctaaactgct atgtcctaac
    55981 cgttttgata cgctaagtat atttactcct tgataaagta aaatagatgc atgtgtatgc
    56041 cttagtccat gcaaagtaaa ttgcttttca atccctaatt cttttaattt tttgcgcaaa
    56101 tatttgctga cggcatttga tgaaactaat ccatttttta agttgaaaaa aacaaaatta
    56161 tgagggtttt taacttcaaa attctcgaat aattcttttt gattgat
//
LOCUS       DAZUMM010000007        54562 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000007 DAZUMM010000000
VERSION     DAZUMM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.007"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..664
                     /locus_tag="ACG8E6_000375"
     CDS             32..664
                     /locus_tag="ACG8E6_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060824.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(798..923)
                     /locus_tag="ACG8E6_000376"
     CDS             complement(798..923)
                     /locus_tag="ACG8E6_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFS5060825.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1057..1950)
                     /locus_tag="ACG8E6_000377"
     CDS             complement(1057..1950)
                     /locus_tag="ACG8E6_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFS5060826.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2132..3058)
                     /locus_tag="ACG8E6_000378"
     CDS             complement(2132..3058)
                     /locus_tag="ACG8E6_000378"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFS5060827.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3143..3904)
                     /gene="pflA"
                     /locus_tag="ACG8E6_000379"
     CDS             complement(3143..3904)
                     /gene="pflA"
                     /locus_tag="ACG8E6_000379"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFS5060828.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4148..6379)
                     /gene="pflB"
                     /locus_tag="ACG8E6_000380"
     CDS             complement(4148..6379)
                     /gene="pflB"
                     /locus_tag="ACG8E6_000380"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFS5060829.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6656..9106)
                     /gene="parC"
                     /locus_tag="ACG8E6_000381"
     CDS             complement(6656..9106)
                     /gene="parC"
                     /locus_tag="ACG8E6_000381"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFS5060830.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9118..11163)
                     /gene="parE"
                     /locus_tag="ACG8E6_000382"
     CDS             complement(9118..11163)
                     /gene="parE"
                     /locus_tag="ACG8E6_000382"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFS5060831.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11329..11763)
                     /locus_tag="ACG8E6_000383"
     CDS             complement(11329..11763)
                     /locus_tag="ACG8E6_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFS5060832.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11890..12528
                     /gene="plsY"
                     /locus_tag="ACG8E6_000384"
     CDS             11890..12528
                     /gene="plsY"
                     /locus_tag="ACG8E6_000384"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFS5060833.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12660..13535)
                     /locus_tag="ACG8E6_000385"
     CDS             complement(12660..13535)
                     /locus_tag="ACG8E6_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFS5060834.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13617..14408)
                     /gene="codY"
                     /locus_tag="ACG8E6_000386"
     CDS             complement(13617..14408)
                     /gene="codY"
                     /locus_tag="ACG8E6_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFS5060835.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14426..15826)
                     /gene="hslU"
                     /locus_tag="ACG8E6_000387"
     CDS             complement(14426..15826)
                     /gene="hslU"
                     /locus_tag="ACG8E6_000387"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFS5060836.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15840..16388)
                     /gene="hslV"
                     /locus_tag="ACG8E6_000388"
     CDS             complement(15840..16388)
                     /gene="hslV"
                     /locus_tag="ACG8E6_000388"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFS5060837.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16425..17330)
                     /gene="xerC"
                     /locus_tag="ACG8E6_000389"
     CDS             complement(16425..17330)
                     /gene="xerC"
                     /locus_tag="ACG8E6_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFS5060838.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17528..18904)
                     /gene="trmFO"
                     /locus_tag="ACG8E6_000390"
     CDS             complement(17528..18904)
                     /gene="trmFO"
                     /locus_tag="ACG8E6_000390"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFS5060839.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18873..20951)
                     /gene="topA"
                     /locus_tag="ACG8E6_000391"
     CDS             complement(18873..20951)
                     /gene="topA"
                     /locus_tag="ACG8E6_000391"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFS5060840.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21073..21930)
                     /gene="dprA"
                     /locus_tag="ACG8E6_000392"
     CDS             complement(21073..21930)
                     /gene="dprA"
                     /locus_tag="ACG8E6_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFS5060841.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21986..22753)
                     /locus_tag="ACG8E6_000393"
     CDS             complement(21986..22753)
                     /locus_tag="ACG8E6_000393"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFS5060842.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22746..23606)
                     /gene="ylqF"
                     /locus_tag="ACG8E6_000394"
     CDS             complement(22746..23606)
                     /gene="ylqF"
                     /locus_tag="ACG8E6_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFS5060843.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23866..24264
                     /locus_tag="ACG8E6_000395"
     CDS             23866..24264
                     /locus_tag="ACG8E6_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5060844.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24501..25043
                     /locus_tag="ACG8E6_000396"
     CDS             24501..25043
                     /locus_tag="ACG8E6_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS5060845.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(25126..25677)
                     /gene="lepB"
                     /locus_tag="ACG8E6_000397"
     CDS             complement(25126..25677)
                     /gene="lepB"
                     /locus_tag="ACG8E6_000397"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS5060846.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25829..26056)
                     /locus_tag="ACG8E6_000398"
     CDS             complement(25829..26056)
                     /locus_tag="ACG8E6_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFS5060847.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(26053..26571)
                     /gene="msrA"
                     /locus_tag="ACG8E6_000399"
     CDS             complement(26053..26571)
                     /gene="msrA"
                     /locus_tag="ACG8E6_000399"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5060848.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26591..27304)
                     /locus_tag="ACG8E6_000400"
     CDS             complement(26591..27304)
                     /locus_tag="ACG8E6_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFS5060849.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(27307..28203)
                     /locus_tag="ACG8E6_000401"
     CDS             complement(27307..28203)
                     /locus_tag="ACG8E6_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS5060850.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(28361..29203)
                     /locus_tag="ACG8E6_000402"
     CDS             complement(28361..29203)
                     /locus_tag="ACG8E6_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5060851.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            29335..29988
                     /locus_tag="ACG8E6_000403"
     CDS             29335..29988
                     /locus_tag="ACG8E6_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFS5060852.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(30051..30578)
                     /locus_tag="ACG8E6_000404"
     CDS             complement(30051..30578)
                     /locus_tag="ACG8E6_000404"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS5060853.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(30598..31545)
                     /locus_tag="ACG8E6_000405"
     CDS             complement(30598..31545)
                     /locus_tag="ACG8E6_000405"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS5060854.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(31557..33440)
                     /locus_tag="ACG8E6_000406"
     CDS             complement(31557..33440)
                     /locus_tag="ACG8E6_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5060855.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(33603..35681)
                     /locus_tag="ACG8E6_000407"
     CDS             complement(33603..35681)
                     /locus_tag="ACG8E6_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS5060856.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(35694..35969)
                     /locus_tag="ACG8E6_000408"
     CDS             complement(35694..35969)
                     /locus_tag="ACG8E6_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060857.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(36331..36786)
                     /locus_tag="ACG8E6_000409"
     CDS             complement(36331..36786)
                     /locus_tag="ACG8E6_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060858.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(36940..38148)
                     /locus_tag="ACG8E6_000410"
     CDS             complement(36940..38148)
                     /locus_tag="ACG8E6_000410"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFS5060859.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(38260..38592)
                     /locus_tag="ACG8E6_000411"
     CDS             complement(38260..38592)
                     /locus_tag="ACG8E6_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS5060860.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            38740..39615
                     /locus_tag="ACG8E6_000412"
     CDS             38740..39615
                     /locus_tag="ACG8E6_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5060861.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(39697..40278)
                     /locus_tag="ACG8E6_000413"
     CDS             complement(39697..40278)
                     /locus_tag="ACG8E6_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFS5060862.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(40284..41543)
                     /locus_tag="ACG8E6_000414"
     CDS             complement(40284..41543)
                     /locus_tag="ACG8E6_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS5060863.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(41545..42591)
                     /locus_tag="ACG8E6_000415"
     CDS             complement(41545..42591)
                     /locus_tag="ACG8E6_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060864.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(42826..43101)
                     /locus_tag="ACG8E6_000416"
     CDS             complement(42826..43101)
                     /locus_tag="ACG8E6_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFS5060865.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(43344..44654)
                     /gene="der"
                     /locus_tag="ACG8E6_000417"
     CDS             complement(43344..44654)
                     /gene="der"
                     /locus_tag="ACG8E6_000417"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFS5060866.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(44836..46065)
                     /gene="rpsA"
                     /locus_tag="ACG8E6_000418"
     CDS             complement(44836..46065)
                     /gene="rpsA"
                     /locus_tag="ACG8E6_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFS5060867.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(46200..46880)
                     /gene="cmk"
                     /locus_tag="ACG8E6_000419"
     CDS             complement(46200..46880)
                     /gene="cmk"
                     /locus_tag="ACG8E6_000419"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFS5060868.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(46929..47564)
                     /locus_tag="ACG8E6_000420"
     CDS             complement(46929..47564)
                     /locus_tag="ACG8E6_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFS5060869.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(47630..49033)
                     /locus_tag="ACG8E6_000421"
     CDS             complement(47630..49033)
                     /locus_tag="ACG8E6_000421"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFS5060870.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(49020..50051)
                     /locus_tag="ACG8E6_000422"
     CDS             complement(49020..50051)
                     /locus_tag="ACG8E6_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5060871.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            50082..50303
                     /locus_tag="ACG8E6_000423"
     CDS             50082..50303
                     /locus_tag="ACG8E6_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFS5060872.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(50468..51205)
                     /locus_tag="ACG8E6_000424"
     CDS             complement(50468..51205)
                     /locus_tag="ACG8E6_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFS5060873.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(51358..52698)
                     /locus_tag="ACG8E6_000425"
     CDS             complement(51358..52698)
                     /locus_tag="ACG8E6_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS5060874.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(52935..54458)
                     /locus_tag="ACG8E6_000426"
     CDS             complement(52935..54458)
                     /locus_tag="ACG8E6_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS5060875.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT        14869 a        11527 c         9308 g        18858 t
ORIGIN      
        1 tatatactta tcaatttaat ttggagatat tatggaaatt caacgtagaa ccactaattt
       61 tttacattta ggctcttttt ttgcaacttt gacgatctta ttttttctat gttattcata
      121 ccttaagacc tcacatgcgc cttctagtgc attaggctgg ttgcttttgg atcattctga
      181 tatcacagaa gccgaagaaa atccgttttt tttgattttg tcttctcttt gcggcttact
      241 gttttctgtt tatttcggtg cctatttttt tcatcgacat cctggtacgt taaacgaagc
      301 ttcaagcgta ttgattgcat ttaaaacgtt ttctttttta gcaattcttt tttattcgtt
      361 taatcacaat acgcttttct tctttatcat gaatggttta gtcgtgattt tatccatttt
      421 tacttatttt attttttcac tcatttttag ccaattgaaa tgtccgctct tctttcgatt
      481 agttcctgtc acctgtttta tttacagctt gttttccttt tttgtccttg ctttaatgcc
      541 actgaccaac ccttcgacga tccaagtcgc aggaaatctg ttggatatgc tcttcatcgt
      601 cagtctaggt gtatttatgt ggcaaagaaa aaacaaacaa aataaaaagc attttgaagc
      661 ctgactatta cgacttcata aatgcttttt tagttttaaa cacttcaatc taaaagaata
      721 acccaagatt ggataaatta ttaatgactc gccattcgtt tcttagtgat tctggattat
      781 caaatggact tcctaatcta atcagctagt tcttcccata aaatgccata cttgactgaa
      841 tcataatatt gattatttaa aaatctaact tttcttatca ctacttcctt tgtcattcca
      901 catttctcac catttttttg cattcctttg tttccagacc atgtagtgaa tctaataggt
      961 ggtagatagg caaaatactc aaataattct tttaaccata aaggaagaga aaggctcctc
     1021 ctgcaagagt cagtataatt tttgcttact gctctcttag gttttactca ctaaaactgt
     1081 tccatattct ttgatataag aaaatgcatg cggtataaat ttcttgctca gctcagaaaa
     1141 gatttgtcgt ttaagaaagg ccatttcctc cattgtcaat gaaggatcca atgttggtaa
     1201 ttcaatctca aaattgtttt tttcgacaat aggtaattct aatgtgaatg attcttcttc
     1261 ctttatagta ttcgtcacaa gtagagactg atggaaaata ctaggccggc gatttttata
     1321 tttattctta cgtatcctgt caacgataac ttctatttga tcaaaaaagt acgattcatt
     1381 tacgtctcca ataagttcgg ctaattcttt gaatgccttc gttacaggaa gctgattctt
     1441 ctgtacaaaa aagtaattag ccaaagtaag cgggaagtgt tctatagagg aaagtgtgat
     1501 aaaacggtac ttctcatttc ctgcagttgt cctcagcaat tctgtctctc ggctgtttct
     1561 tactccataa aaatacgctt ctttccgttc atcttcgaaa caatacgccc aaataacttc
     1621 tcccatcgct aatttttgtt catcaacaga gagtctcttc aattgttcag ctatggtttc
     1681 agcagccgaa gtggcttgtt gcaagtaatc tttaggatca aatattggaa agttcaaatg
     1741 atatcttcgt tcttccctct tgattaatcc tgcatcgatc aataaatcaa gaaaatggga
     1801 aaatttattt tgcgaaaatt cttttttcaa ttctcttaga atcgtttctt gttctcgatg
     1861 ttcatataga taatcggtca atgaagaaaa aagcggatca tcggtcagtt tttgataaaa
     1921 gttttcctta ttttttgaat aatagttcat atatgttctc ttttctatcc gttttatttt
     1981 acttaaaagg cttttacgta aaaaggagga agaacaagag tcataattga ctgttattct
     2041 cctccatttc ttcggccttt agtatgttct atgtttcttt ttatatcaat cgcttatcca
     2101 ttcattttga atgcttcata ctaccatttg attagctaaa tacagccgtc aattgaggta
     2161 cgacttgttt tttacgtgaa acgacacctt taagcaaagc acggttgttg gtcaaagttg
     2221 tttggaaagc ttcttctact ttttcttttc ctttaccagc aacaagtaat tctgaatcac
     2281 tgtctaagat gtttgtgaca actaatacga acagatcgta gccatttgct ttcatttcat
     2341 cttccattgc tgcttctagc tctgcttgtc gatccatcac ttcattaagg tccactgtat
     2401 tgacttgagc gatacgaacg ttggaactgc ccattgggaa actttttgca tccaaatcaa
     2461 gaagaaccgc agctgattta tcacttagat ttgttcctgc tttcaacatt tcaagtccat
     2521 aagtgtttag atcgacttca gcgattgctg caagttcttt tgcagctgct tcatcttcag
     2581 gcgtacatgt tggtgatttg aacaacaatg tatctgaaat gatagctgag agcatgattc
     2641 ctgcgatttc tttaggaatt gttacttggt tttctttata tagttttaaa ataatcgtac
     2701 ttgtacatcc aactggttct gcacgatagt acaatggatt agctgtttca aagtttgcaa
     2761 tacgatggtg gtctactaca gctaaaatat ttacttcgct gatatctgaa atactttgtt
     2821 ggaattcatt atgatccaca agcatgacat ctgcattttc tgctttagct gactctacta
     2881 ctcgtggtgc agatagctga aagtggtcta atgcgtattg tgtttcttca ttaggtgtac
     2941 ctagagcaac tgcttctgtt tctttaccta attcattttg caagtgtgaa aacgcaatgg
     3001 ctgccccgat tgcatctgta tctggatttt ggtgaccaaa aactaaaatt tttgacatgt
     3061 cattcgctcc tttgtgattt ctcctattta ataatagcaa aaaaacggaa agaagcaata
     3121 cttatcttct ttccgatgcg acctatcgcg ttaaatattt tgtatactct tctgtatgaa
     3181 gcaatcgttt agcattttcg acacgttctt ttgtaggtgg ttcgatccct tctaatggat
     3241 atttcaaacc aagtgtttcc cacttgtatt ttcccatcgt atgataagga agaatctcta
     3301 ctctgtctac atttgacaag gtttgaataa agttgtctag tcggatcaaa tattcatcat
     3361 aatcacttcg ctcaggaact agtacatggc gaatccaaac cggtttgttg atttttgaca
     3421 aatattgagc catttctaaa atattttcat tgtcccaatg cgttagtttt ttatgctctt
     3481 cattatcgat gtgtttgatg tcaaataaca gtaaatctgt atacttcatc aattcttgaa
     3541 aacgtgaaaa gaaaggttct tcatacgtaa aaggttttcc gcatgtatcc aatgtagtat
     3601 gcatcttccg ctcttttgct tttttaaata gttcgatcaa aaaatcgatc tgcaatagag
     3661 gttctccacc actaacagta ataccacctt tatctcccca gtaaggtctg aagcgttctg
     3721 ctttatctaa aagttcatct gctgtgtatt ctttccctcc gccaatattc caagtatctg
     3781 gattgtgaca aaattggcaa cgcatgcggc atccttgcat aaacacgaca aagcgcagtc
     3841 caggtccgtc tactgaaccg aaagtttcga tagaatgaac atagccgatt gttttttctt
     3901 ccatttaagt tcacatcttt tctttgattt cattttaagt acaagttagc tgtcgaagcg
     3961 aagctttctg aacaaaattt ttcgcaattg ccgcttcgtt atgtgtaaaa tttacaggga
     4021 cggaacatac aagtcctgcc cctgtaaatc gtttctttca aataagcata ctgattattc
     4081 gattttgatg cgatggctat tcaacgtcct ttcgacgcta agtcgaaaaa cttttttcac
     4141 gaacttttta cattctgtcg tgtgaagttc tcatcaagac atctaattgt tgttctcttg
     4201 tcaagtcacg gaatttaact gcataaccag atacccggat cgtcaagttc ggatatttca
     4261 ttggattttc cattgcatca cgcaataaat cattactgaa tacattgaca ttcaaatgat
     4321 aagctccttt tgagaagtat ccatctaaga cattacgcaa gttttcgatt cttgtatctt
     4381 gttcttttcc aagacctgaa ggattgattg tttgggtatt tgaaattcca tccaatgcac
     4441 agccgtaatt cattttagct gttgagttca ggctagctaa taacccactt tgttccgcat
     4501 tgtaacttgg atttgctcct ggcgcaagtg gttttccagc ttcacggcca tcaggagtat
     4561 ttcctgtatt tttaccgtaa acaacatttg aagtaatcgt caataatgaa gttgttggtt
     4621 ttgagttgcg gtaagtttta tgacgtttga tttgtcccat gaagtattcc aagatccatg
     4681 aaactaattc atctgcacgg tcatcatcat ttccgtattt agggaagtcg ccatccactt
     4741 caaaatcaac tgctattcct ttttcattac ggattgcttt tacttttgca tatttgatcg
     4801 ctgacaagct gtctgccgca tgagagatcc cagcaatacc tgtagcaaat gtgcgttgca
     4861 agtcagaatc catcaaagcc agctgagctg cttcataaga atatttatca tgcatataat
     4921 gaatgatgtt caacgtattg acatatagtt ctgctaacca atccatcata ttcatgaagt
     4981 tatgtttcac ttcttcaaag tctagtattt ctgatgtgat tggacgatat tctggtccaa
     5041 cttgggcgcc agtcacttca tcgactccgc cattgatggc atataatagt gttttagcca
     5101 agttagcacg agcgccaaag aattgcatgt cttttccgac aactgttcct gatacacagc
     5161 aagcaatcgc acaatcatca gaaccccatt gttcacgaag ctgattatca ttttcgaatt
     5221 gaatcgaaga actttcgata gcgattctgc tagcatatgt tctgaatcct tctggaaggt
     5281 gttcagaata caaaacagtc aagtttggtt ctggtgaagg tcccatattt gttaatgtat
     5341 gaagcagacg gaaatcattc tttgtaacta atgaacgacc atctaatccc atacctgcaa
     5401 tggataaagt agcccagata gggaatcctg agaataattg gttatattca ggtgtacgcg
     5461 cgaatttgac catacgtaat ttcattacta agtggtcgat caattcttgt gcttcttttt
     5521 ctgtgattac tccattatcc aaatcacgtt ggatatagat atcaaggaat gctgagatac
     5581 gtccgattga catcgcagca ccattttgtg atttgattgc tgctaaatat ccgaagtata
     5641 gccattggac agcttctttt gcatttgcag ctggtttaga aatatcatag ccatatttcg
     5701 cagccatttc tttcatttgc gctaatgcac gatattgttc agtgatttct tcacgtaaac
     5761 gaatcacttc ttcactcatt gtatgataac cagtcatatc atgatctttt ttcttttgtt
     5821 ccatcaaata gtcgattcca tacaatgcca cacgacgata gtcaccgatg atacgtccgc
     5881 gaccgtaagc gtctggaagc cctgtaatga ttttgttttt acgagcagca cgcatttcag
     5941 cagtataagc gtcaaataca ccttgattat gtgttttgcg ccagtcagtg aaaatatgtg
     6001 tcaattcatc gtttggtgta tagccattgc tttttagcgc attgttagcc atattgattc
     6061 cgccgaacgg catgaatgct tgtttcaaag gtttgtctgt ctgtagaccg acgatttttt
     6121 ccaaatcttt atccaaatag cctgcatcgt gtgaagtaat agttgatacg atttctgtgt
     6181 ccatatcata gacaccgtta cgctcgaatt ggatatcatt taacttttgc aattcatccc
     6241 ataatttttc tgttgcttct gttgggcctt ccaaaaagct gtcatcaccg cgatattcag
     6301 tgtagttatt ttgaataaag tctctagtgt ctacgccttc tttccatttg gtacctttaa
     6361 atccattcca ttgctccatt gtatggacct cctaaatttc tgttttatgt aacagttttt
     6421 acgtgattag tataacacag caccctatct ttgcaagggt tttcttgttt tttatttaac
     6481 aatttttatg ttttatttac atatatttaa atcaacgttg acataccaac atttaagcat
     6541 tgtgaacact ttcatctttt tttgcaaacg tataaggatt tattaaaaat gaatgaaaaa
     6601 gagacgccac ctgtattaat acagatggcg tctcttcatt aaaatactac aacagttacg
     6661 actcgatgaa aatctcagga gcatcgataa cttcgatgac ttctccacct tttttctcat
     6721 ccatcgcaaa tgatccattc gatgttcgat cacctatagg gaaatcagtt gggtcaatcg
     6781 ttaactgctg ccctttttga ttcatcaagg ttatttcttt tgatcttcct tccgacatat
     6841 agataatacg gtgcggattt tttttcagct cacgcatgac cgtcactccc cgcttcgcac
     6901 gtgaagtttg gctgatttct tgagcaagca tgcgtttaac agctcctcgc tgagtaacga
     6961 ttacgatcgg cgtatcacct tctgctaaca caagcagccc gttgataatc ctatctccat
     7021 ctttcaaatt catagacttc actccagctg cctttgctcc aacaacggga acatctgtta
     7081 aaggatagcg caaacccatt ccttgatgcg ttactaagaa aacatctaag cctggctgat
     7141 cttctttcag atacacagca atcagttcat cttcttccgc ctttagcttc atacagctga
     7201 gcggacggct cttataggtg cgccatggtt cgaactctgt cattcgtgtt tgcttgatca
     7261 atccattctt gctgatgaat acaaatgttt tctctgcttg aattttttga tatgggaaaa
     7321 cagctaaaat cgattcgtct acagacagat tggagattgt ttgagaaatg tgctcaccag
     7381 catctttcca tttcaaatcg ggtagttcat ggacagggcg gtagatcaca tttgctttat
     7441 ttgtgatcag aagaacatgg tccaatgtac tcaattcacc tgagtatagc aagtagtcgc
     7501 cttctcgcat cccgatttct tctggtttgg aagcactata agaacgaagg cttgttcgct
     7561 tcacatagcc ttctcgcgtc acactgacaa ttacgtcttc ttgtgccact aagacttgcg
     7621 tatcaatttt gatttcttcg atttcgtctt cgattgtcgt tagacgagcg gaagaataat
     7681 tttttttcac ttctcgcaat tcttttttca taacagagaa aagctctttg tcatttgaaa
     7741 ggattttatt caactctgtg atttgcgcaa tcaagctttc ggattccttt tgaagctctg
     7801 tgatatccgt gttcgtcaaa cggtataatt gcaatgtgac aattgcttct gcttgttctt
     7861 ctgtaaattg aaagacgtcg actaaattct ttttggcgtc ttttttgtct ttactttcac
     7921 gaatcgttgc gattacttca tccagaatcg ataaagcttt catcaatcct tctacgatat
     7981 gctgtctttt ttgagctttg gctaaatcga attgactacg ttttgttatg acttgctttc
     8041 gatgctcgat ataacttctt aaaatgtctt tcagtccaac ttgatgcgga gtcatatggt
     8101 cgatagcgac catattgaaa ttgtaattga tctgtagctc agtgtttttg aacaaataat
     8161 tcaaaattcc ttgagcattg gcgtcttttt tcaattcgac gacaatttgc agacctgttc
     8221 tatcactttc atctcgtact tcagctattc catcgatttt cttattcaaa cggatctcat
     8281 ccattttttt caccaaggtc gctttattta cttcatatgg gatttctgtg atcacgattt
     8341 gctgtttatt acccttcatt gcctctatct ttgttttcga acgaagaata acttttcctc
     8401 gtcctgtttc ataagctttt ttgatttcat cttttccttg caagatccct cctgttggaa
     8461 agtcaggtcc aggaatatat tccatcaatt tttcaaggct cgcttgtgga tgatcgatca
     8521 tatagattgt gccatcgatc acttcagcta agttatgagt cgggatttct gttgcatatc
     8581 cagcagatat acctgtcgat ccattgacga gaaggtttgg gtatcgagct ggaaggactg
     8641 ttggttcttt ttctgtatca tcaaaattcc acacgagatc gactgtttct ttctcgatat
     8701 ctgctagcat ctctccactt aacttggata atctggcttc agtataacgc atcgcagcag
     8761 gtggatcacc gtccatactc ccgttattcc cgtgcatttc gatcagtact tcccgcaact
     8821 tccagtcttg gctcatgcgg accatcgctt cataaatact gatgtcacca tgaggatgat
     8881 aattccccat gatatttccg acagattttg ctgatttacg gaatcctttg tcatacgtat
     8941 tgccatcttt gttcatggca aataatatcc ggcgttgcac tggcttcaat ccatcacgga
     9001 tatcaggcag tgcacgttct tggataatat atttggaata acgtccgaag cggtcaccca
     9061 tcacttcttc taaagttaat tcttgtactt cttgtcgatt ttccatcagg tgacctccta
     9121 ttctacttca aataactgat tgttttcatt tttatctgct cgttcttcat ctaataaatc
     9181 attagaaact gaaggagaga tttcagtatc ctctttttta tctaagatac tgccatcttc
     9241 ttcgagcgta aattgcacgt gattttcgat ccattttctt cgaggttcta ctttgtcacc
     9301 catcagtgtg gttactcgac gttcagcttg cgcagcatca tcgatacgga cgcggatcaa
     9361 cgtacgggaa gtcggatcca tcgttgtttc ccacaattgt tccgcattca tctctccaag
     9421 tcctttatat cgctgcagca tatatccttt gccgactttt ttaatcattg ctgccaattc
     9481 gtcatctgtc cacgcatatt caatgacttg cttctttcct tgtcctttgg atactttata
     9541 taaaggcggt aacgcgatat atactttccc agcttcaatc agcggtttca tataacgata
     9601 aaagaaagta agcagtaata cttgaatatg ggcgccgtct gtatccgcat cggtcatgat
     9661 gattactttg tcgtaattac agtcttcgat agaaaactca ggaccgactc ctgcaccaat
     9721 cgtatagatc atcgtattga tttcttcatt tttcaagata tcttgcattt tagctttttc
     9781 ggtattgatg acttttcctc gtaaaggtag aattgcttgg aactttctgt ctcttccttg
     9841 tttagcagat ccgccagcag aatccccttc gactaaatac aattcatttt tcttagggtt
     9901 tcgggattgt gcgggtgtca attttcctga aagaagagat tcgccttttt tacgcttctt
     9961 cccatttcgg ctttcttctc tggctttacg tgctgcttct ctcgcttctc tggctttgat
    10021 tgctttacgg accaacatct ggctcatttc gctattttcc tgcaaataaa agcccatttg
    10081 ttcactgatg acgttatcta ctacggtacg agcaactggc gtaccaagtt tttcttttgt
    10141 ttgcccttca aattgcagaa gattttcagg tacacggata gaaagtacag cagctaaccc
    10201 ttctctgaaa tcactacctt ccagattctt gtcccgttct ttcagcaaac caacttttct
    10261 cgcatattca ttataagatt tcgtcatcgc cgctttcata ccagcttcat gggttccccc
    10321 atcttttgtc cgaacgttat tgacaaaaga taaaacattt tctgaataac cgtcattgta
    10381 ttgataagcg acctctactt cgataccttc tttttctcct gaaaaataaa caactggtgt
    10441 caatgtatct ttttcttcat tcagataatc aacaaattct ttgattcctt cttcgtaatg
    10501 aaaaatttcc ttgactggtt cttctccacg aagatcagaa agagagattt taacgccttt
    10561 tagaagaaag gcagattctc ttaaccgttc ggctaaaatt tcatatgaaa atttcgttgt
    10621 actgaatatt gtatcatctg gtaaaaaatg gacagtcgtc ccgtttgctt tttttgtttt
    10681 tcctatcttt ttcaaagtac cgtcaggttt accgccgttt ttaaatttct gctgatactc
    10741 tacgccatct ctaacgatcg ttacaattag ccatttcgat aaggcattta ccacactggc
    10801 tcctacaccg tgcagtccac cagaagtttt gtaccctcct tgaccaaact ttccgccagc
    10861 atgcagcaca gtaaaaatca cttcaactgt tggtattccg gaagcatgca ttcctacagg
    10921 catcccacgt ccgctatctg caacagttat actattgtct tcatggatgg tcacatcaat
    10981 ctcactgcca taacctgata atgcttcgtc aactgcatta tcgacgattt catagactaa
    11041 atgatgcaag cctcggctgt cagtggatcc aatgtacatc cctggtcttt ttctaactgc
    11101 ctctaatcct tccaatactt ggatggacga gtcattgtat tcgttgttta ctttttttgc
    11161 caaggcaaac gctccttcaa taatactttg cggcaaaacc gcaccacgat tatcatacta
    11221 gaaaaggtcg tgaaaaaaaa gagggtagac gagaaatcaa ttttaattat ctcctaaccg
    11281 ctttttcgac attcccctct atcgatcatc tcttctttgt ggtcattttt atttttggtt
    11341 ttctttcaac ttaccaagtt caatcttaat acaacgatcc attacgatat ctgtacgtcc
    11401 tgcttttctt agcatctcag ctgcttcttt gttttcaatc cctaattgtg cccagaacac
    11461 tttagcatca gttttcaaaa aatcacctgc tatttcaggt aaaaattcac tccgtctaaa
    11521 tacatctacg atatcgattg gatcatcgat ttctgtcaaa ttcgcataga ctttctcgcc
    11581 tagaatttct tcaccagcca ataatggatt tactggtatg attttatatc cattgtcttg
    11641 caatacctgt gcaaccatgt aacttgttcg ttctggttta ttgctcaagc ctacgacggc
    11701 aatatttttt gcttcttcca aatagttgaa aatcgtttct tgtgttggat tttttacaga
    11761 catttctgcc accccttgct tttcttttta ttccatctat tatacttgta tctttcattt
    11821 tttcaattgt tcctgttacc tattcatgct aaaataaagg atagttttca acttgaaaga
    11881 gggtaaaaaa tgaaattgat cttattactt attttagcat atcttttagg ttctattcct
    11941 tccggtgtct ggattggcaa attgtttttc aaaaaagata tccgacaaca cgggagcggt
    12001 aatactggaa cgaccaatac atttcgtgta ttaggcaaaa cagccgggat tgttgtcctg
    12061 cttatggata ttttaaaagg tacattggct acttgtttgc cgatgatctt ccacctttct
    12121 gggattaacc ctctttggtt tggggtatgc gccattcttg gccatacatt tccgattttt
    12181 gctgggttca aaggaggaaa agctgttgcg acaagtgcag gaatgctttt aggatttaat
    12241 cctgtatttt ttgtttattc ttgtatcatc tttatcgtta gtttattttg tacaagtatg
    12301 gtgagtttga gcagtatgat cagtgcagta ttgatcactt tatctacgat cgtccttcct
    12361 tatgtagctc ctgtgatttt ggctaagccc aattggttac tgacgatcat tgccttttta
    12421 gtttcttcct ttatctttta tcggcataaa gacaatatca agcgtatcag aaaccatact
    12481 gaaagtcgaa tcccgtttgg tttgaatagc agcaagaaaa aatcctgata ctttttcatt
    12541 ccaaagagat aaaacgggag tgtgacaaat cgatgtcaca ctcccttatt acttacagga
    12601 tagctttttt acacaccttg gtaaacggtg ttccctgcta acttctcggt cacaacctct
    12661 tacttcactg tgatggaata ttttgtcttg aagatttcat gggcacctag ttgattgatc
    12721 cctacttttt ccatcaagtt tccactactg ttcaccgtgt cagcgattcc gcaccacggt
    12781 tcgatacaaa cgaaaggcgc ttcttttgga taaggagacc agatccctac ataaggcata
    12841 tctttatagc ttaaagttac gctatgggaa gatttttcac ttcgaatggc gtacgcattt
    12901 agaccccttg tttcaaaaac caaggcatct tgctcaaaaa gttcacgatt caaagccaga
    12961 ttcgtatttg tctgacctaa cgtcttttga tccatatcga tgaatgggcc tttcaatggt
    13021 aggcgtaatc ttgatttcaa tggagaaaat gccagataga aatcttcaaa tgacaatcct
    13081 tcttcaagag gtacattgaa agcaggatgt ccaccgatag aaaagaacat ttctttttca
    13141 ccggtatttg ccacttgata atgaaccgtg atgccgtctc ctcctaattc atagctgatc
    13201 actaattcaa aatcaaatgg atacttttct aatgtctccg gcgtactcct taagatgaaa
    13261 ctagccattt cttgtccatg ttcaatgact tcaaaatcca tatcccgagc aaatccgtgt
    13321 tgccccattt gatatgtttt cccttgataa acatactgat catcttttaa acgtccaaca
    13381 aaagggaata aaaccggtgc atgtctgccc caatacgctg gatcaccttg ccagatatac
    13441 tctaaaccat gttcttttga tttcagactg atcaattccg caccgtgttc agcgatttcg
    13501 gcgatcagct ggtcattttg aataataaca gtcattgcca ttcctccaac ttcgacttac
    13561 tttttaaaat gggaaggctg gaacaaacat ttctatctgc cccagtcccc ttacttctaa
    13621 taactgtgtt ttgctaactc gtctttaaaa cgggaattca atacttttaa ataagttccc
    13681 ttcatgccta acgaacgaga ttcaatgatt ccagctgatt ccaattttcg taaagcattg
    13741 acgatcacag aacgagtgat gccgatacta tcggcaatat tagaagcagt cagacgacct
    13801 tcatctccat ccaatgcttc aaagatagct tgtacagcct tcaattcgct ataagataat
    13861 gtgttgattg ccatttggac agccgttgca ctccgtacat ctgcttcgat gcttcgagac
    13921 ttctgataaa ggatctgcat accgacaacc gtagctccat attctgctaa gactagatcg
    13981 tcatctgcaa acgattgttc catacgagat aagataatcg tacccaaacg ttcgccagct
    14041 ccaaagatcg gaacgacagt cgtcagacca aatggatatt tctctcttga ctctaccgga
    14101 aatgcagtaa tatcactagt aattgggata ttagcttcag ttttcatcaa ctgtgccact
    14161 gtatcagtat aagattcagg aaattgtttc tcaacaaaca ttgttttgat tcgttcattg
    14221 ttcacatcgt gacgttcatt ataacctagt aatactccgt cacttcctat gatataggtg
    14281 ttgctgtcaa gaataccacc tagtgtgaca gccattttat cgtaaggcaa atctgcttgc
    14341 atgtcaaaag tattcttttg ctgcaaaagt gtgtttatct tgcgggtttt cgttagcaag
    14401 tctgtcattg tcgtcctccg attttttata aaatatagcg acttaaatct tcatttgccg
    14461 caattgagcc aagtttgtca tccacatatg cttctgtgat cgttatttct cccatttgca
    14521 tatctggtgc ttcaaataat agatcttcta gtagtttttc caaaatggta tgcagtctgc
    14581 gtgcgccgat attatctgtg tcacggttga catcgaaagc gatttgagcg atccgttcaa
    14641 ttgcttcttt tgtaaacgta actgacacat tttctgtacc gatcaacgcg acgtattgtt
    14701 ttactaaagc attatttggt tcggtcagaa tcttcacaaa atcttcagct gtcaaatcat
    14761 ctaattctac ccgtatcggg aagcgtcctt gaagttctgg aattagatca cttggtttag
    14821 acaaatggaa agctcctgaa gcaatgaata ggacgtgatc tgtttggatg atcccgtatt
    14881 ttgtgtttac ttgtgaacct tcgacaattg gcaatatgtc tctttggacg ccttctctgg
    14941 atacttctcc agattgctgt gatttagaag tgatcttatc gatttcatcg ataaaaataa
    15001 ttccactttc ttccgctaat ttgattgcct cactatggat atcagcatcc ttgactaatt
    15061 ttgctgattc ttccttgatc aataactcta aagcttcttt gactgttacc gtccgttcca
    15121 cttttttctt cggtgtcaaa gcacctagag tttcatttag gtcgatcccc atttgctcca
    15181 atccattgtt catcattggt gcaggtttct tttggtcttc aacttcaatg gttacttcac
    15241 gactatccag taaacccttt tgtagttgtt ccaagactgt acggcgattg attcgaatat
    15301 cttctgtcac ttcttcttgc ggttcagcgg tattttgctg actagcatta aaaagattca
    15361 tcatttgttc aaattgatta gtggattgtt tctgttcttt tttgatacca ggtaccaaga
    15421 tttttaccaa acgtttttct gcattttttt tagcacttgt gtatacacgg ctgtactgct
    15481 ctttttctac gatttggatc gcattttcta ctaaatcccg aaccattgat tctacatctc
    15541 ttccgacata accgacttca gtaaattttg ttgcttcgac tttgacaaat ggtgcattga
    15601 cgatttttgc caaacgtcgt gcgatttctg ttttaccgac ccctgtaggt ccgatcatca
    15661 gcatattttt tggtgtaata tcttgttgca ttttttcatc taactgcaaa cggcgatagc
    15721 ggttacgcaa agcaacggca acagattttt ttgctgcgtg ctgtcctacg atatattcat
    15781 ctaattcact tacgatttgt cttggtgttt gttttaattc atacatatct actcacctct
    15841 tacagttctt ctacgataat attatgatta gtaaagatac agatatctgc ggcaatattt
    15901 aacgcatttt ctgcaatttc tttcgcactc atgtcgtttt gagcatagtt tttcattgct
    15961 cgggctgcag ccaacgcaaa attccctcct gatccgatag ctaagatacc atcatctggt
    16021 gtgataactt ctcctgtacc tgaaacaaga agcatttctt tatcattcat tacgatcagc
    16081 atcgcttcaa gtttttgcat agattgctgt gtccgccatt cttgagctaa ttcgacagct
    16141 gcgcgttgta gattcccgtt atattcgttt agtttgcctt caaatttttc ttctaaagta
    16201 aacgcatctg caacacttcc ggcgaaacct acaacaactt ctccattgta gatacgtcgt
    16261 actttctttg cagtgccttt catgacaaca gattcaccca ttgttacttg gccatctcca
    16321 gccatagcaa atttcccgtc tttttcgacg gcacagatag tggttgaatg aaatggtgat
    16381 tcgaccatgt ttgtttcctc ctaaatttgt tcttttcctt atttttacgc tcttggatgg
    16441 aaagaacgat agtttttttg taaactttct tttgttacat gggcatagat ctgggtagtc
    16501 gataaattgg catgtcctag caattcttgg actgtccgca aatctgcccc attatttaat
    16561 aaatgagtag caaatgtatg gcgcagcatg tgaggatgga tatcactatc caaactgctt
    16621 tttttgatca tttgattcaa gacatattca attccagtag gcgtgattgg ttttccatga
    16681 tgattcacaa aaacgatatc atgctcttcg tgatattttg tcatcaatac ctggcggcct
    16741 tccctgaaat attcttgcag agcatcttga gcaaaagaac ctagaggagc atatctctct
    16801 ttgttccctt taccatggat caacatgacc tctgaatcaa aatcaatgtc tgataccgac
    16861 aaactgctac actcacttaa acggatgcct gacccgtaaa ggacttccag taaagcgcgg
    16921 ttcctcaaat ccaaaggact atctcctgct acgctgtcaa aaagtgcttg catttcattt
    16981 tcataaaaaa aacgtggcag tctcaaattt ttctttttga gatgaacgta agaaaatgga
    17041 ttttctttta tcacttcttc tttgagaaga tactgataaa acgaacgtag gctggctatc
    17101 ttgcgactga tagaatttct gctatagcta ggacgatgat cagctaatgc actcaaatag
    17161 acacgaaccg taaaatattc cgccttcagc agatcagcgt ctccagattc ctccaaaaaa
    17221 tgtttgaaat ggagaatgtc ttcttcatat gcttctttcg ttttttctga atatcctctt
    17281 tcgacaatca aataacgcaa aaatttttgt tcccacgtgg cttctgtcat tttctcacct
    17341 tccttttgtc ccagagaata atttagcaca gcgtttataa aaagacaaat aaattgtcag
    17401 aatttaacca atatttctaa gtttgttcac aattggcgca attcctcaca ttttgtttga
    17461 gaaattctta agaaaaacgc aagaaaaaga gcgagactat ccgtctcacc ctcttcatgg
    17521 agtttcctta ctcatttggg attgcagttt tttctaattc tgtttttatt gcctcattag
    17581 cttttaaagc tcgttgtgca attgcttcat atcttgcttt tttgtctcga atgcgctctg
    17641 gcagttcagg aaacagacca tagttggcat tcatcggttg gaaatgtttt ccttccgcat
    17701 gagtaatgta gtaagccata cttccaatag ctgtttcttg tggaaactcg actggttcca
    17761 tttcttttgc taagcgtgcg gcgttgattc cagcaagtag tccgctcgct gcactttcaa
    17821 catagccttc tacaccagtc atctgacctg caaagaacag atcttcgcgt ttcgttgatt
    17881 ggtaagtcgg tttcagcaat tctggcgaat tcataaagct gtttcggtgc atcacaccat
    17941 agcgaacgaa ttcagcattt tctaacccag gaatcatccg gaaaacacgt ttttgctcgc
    18001 cccatttcaa gtgagtctga aagcccacga tgttgtataa agaagctgca gcattgtctt
    18061 gacgcagctg gataacagca taaggacgtt tgccagtttt aggatcttcc aatccaactg
    18121 gtttcatagg tccgaaaagc atcgttttga atcctcgctg tgccattact tcaattggca
    18181 tacatccttc aaaatatttt tctttctcaa aagatttaag ttctgccact tcggcattga
    18241 ttagtgcctc atggaaagct ttgaattcct cttctgtcat cggacagttc aagtaagctg
    18301 cttctccttt gtcatagcgg gacttcaaat atactttatc catatcgatc gtagctttgt
    18361 ctacgattgg tgcagctgca tcatagaaat aaaaaccatc tgaaccattg aatgctttga
    18421 tgctttctgc taatggatga gaagtcaaag gtcctgttgc tactacggtg attccttccg
    18481 gtatttcagt aatttcttcg tttatcactg ttactaatgg atgctctttg acttttcggg
    18541 taattgtttc agaaaaagaa tcgcggtcaa cagctaaggc accaccagct ggcactgctg
    18601 tttcatctgc cgaagtgatt actaccgaat tcaatcggcg catttcttct ttcaataacc
    18661 ctactgcatt agccaaactg tttcctctta atgaattgga acatactagt tcagcaaaat
    18721 tacttgtgtg atgcgcctca gtcgatttga ctggacgcat ttcatataaa cggacaggga
    18781 ctcctgcttg tgcgatctgc caagcagctt cacttccggc aagccctgct ccaacaacat
    18841 ttaccatttt caatgaaaga acctcttttc tattattttt ggatgtcttc ttcgtaatct
    18901 ccatttacac agactacttg tttaccgcct ttgactttct tctcgaccag atatccgtca
    18961 cattttggac aatttctgcc cactggttta tcccaagaag tgaattcaca ttctgggaaa
    19021 cgggaacaac catagaatag tctgtttttc ttagattttc gttcaatgac ttgtccttca
    19081 tgacatactg ggcaagtcac accgatttct tttacgattg gtttcgtatt ccgacaatca
    19141 ggaaaattac tgcaagcata aaacttccca tatttcccta attttatcac catcggatgt
    19201 ccacagacat cacaatcgaa tcctgctggt tcatctttga tttgaatttt ttcgattttt
    19261 tcttccgcat tcgtcagttc tttttcaaac ggtttataga aacgatcaac cactttgacc
    19321 cacgcttccg tgccttcttc aattttatcc aaatctcctt ccatttctgc tgtgaaatgg
    19381 atatcgacga tttgagggaa gaattcacag attaatgaat tgacaatttc tcctagttca
    19441 gtcggctcaa atcttttttg tgtcaatttt acatagtagc gtctttggat cgtatcaagt
    19501 gttggtgcat aagtggaagg tcgccctacc ccgttttctt ctagtgcacg aatcaacgta
    19561 gcctcactaa aacgtgctgg tggttgagta aaatgttgtt ttggttcaat atcaacagat
    19621 tggactacat cgccttcttc aagttctgga agaatatttt ctttctcttc tttgccgtca
    19681 tctctacctt ccacatacac ttgcatgaat cctttgaatt tgatttttga accgttcgca
    19741 atgaagatga caccattttg ttctaatgtc actttcattg tatctaaaat agctggtgtc
    19801 atctgactag ccacaaaacg tgaccagatc aatgtataaa gtttcaattg atctttatct
    19861 aaatattgtt tgagttcatc tggtgtacga agcacactcg ttggacgcac agcttcatgg
    19921 gcatcttgtg cgccttgagc atttttgatc tttcggtgat catgtctaga aaattctttt
    19981 ccatacgtat cttcaataaa tgctgcggcc tctgcttttg ctgtatcggc aatccgggta
    20041 gaatcagtac gcatgtatgt aatcaagccg actgttcctt gtttacccaa agtgattcct
    20101 tcatataatt gctgtgctac catcatggtt tttcttgttc gaaagtttaa cttacgggca
    20161 gcttcttgtt gcaggctact tgtagtgaaa ggattcgcag ggtttctttt gcgctctttc
    20221 ttttctactt ttttaacttc gaagtctgtt ccatcaattc tagcagtgac ttcttttaca
    20281 ctttgtgcat ctggtaattt ctttttctta ccgtcgattc cccagaaatt tgctttgaat
    20341 ttcttgcgag cttttttaaa attaccgtcg atgctccaat attcttctgg aacaaattct
    20401 cggatctctt tttcacggtc aataataatt tttaaagcga cagattggac acgtcctgca
    20461 cttaaccctt tcttgacctt acgccagaga ataggactga tcgaataacc aacgattctg
    20521 tctaaggtac gacgagcttg ctgtgcatct actaaatcta catcgatcgt tcttggttcc
    20581 ttaaaagctg ctttgactgc gtcttttgtg atttcattga agaccacccg atttttttct
    20641 ttcagatcca acccaagtag gtacgctaaa tgccaagcaa tcgcttctcc ttctctatcc
    20701 gggtcacttg ccaagtaaac tttttgagct ttttttgctg cggctttcag gcttttgatg
    20761 acatcgcctt ttccgcgtat agaaatataa tggggttcat aattgttttc aaaatcgata
    20821 cccattttac ttttcggtaa atctcttata tggccaacac tggccattac tttatagttt
    20881 cgtccaagat atttctcaat tgtcttggct tttgcaggtg attctacaat cactaaatat
    20941 ttatacgcca ttactgaatg gactcctttc cattgtcgtg ttcctactat taaaagagga
    21001 ttcatacaaa tcgtagctaa tcatatccat tcagaaaacg tttgtcaaga aaagcaaatg
    21061 aaaagtatgg tgttattctt taaattctgc caagatatct tcacccgaaa ccgtgcaaac
    21121 tgcaccgtct tggatcaaat gatgacatcc ggtagaatgt tctgttaaaa tatttcctgg
    21181 aacagaaaaa actgttcttc caagatccaa tgcttgctgt gctgttatca gagagccgct
    21241 ttttttcttg gcttcaaaga cacatgttcc tttagacaaa gctgcgataa tccgatttct
    21301 cattggaaaa caatgctttt tcgggccgga ttgaggagga tattcactca aaagaagatg
    21361 atcatttgca atcacctctt gcaagtcacg attttcagca ggatagactt gctctatccc
    21421 tgttccaatg actgcgattg tccgtccacc atgagagatt gctgcttggt gaccgtatgt
    21481 atctatccct ttcgcgcagc cactgacgat cacatatcca gcttctatca ctggttttag
    21541 taaataataa actgtttttt tggctaaagc agaagtgtgt cttccaccaa caacgcttaa
    21601 catgttacaa gaaagtaaag atagatcacc attataaaac agtacaattg gcggataggt
    21661 caagtgaagc agctgagggg gatatttggg atcaaagcaa gtgataaacg actgtttcga
    21721 ttggattttg ccccagttag cgtgaatttc ctgccaactt ttgcgaaaca gctcccggtg
    21781 tttggttatt ttacctatct taatgatgtc ttcaaatccg atttctttat aaggatactg
    21841 catcatccag ttagcaagtg cccattttcc ttgactactg attccttttg tataagcaag
    21901 tttcaataaa ttttcttcta tttgatacat cataaaaccg cctttcgtct ctctttctaa
    21961 taagatacgt aaaagcggct gttttttatt gtaaatattt ttttacaggt gcaaaacttt
    22021 tacgatggat tggcgtaatt ccttgtcgtt caagtccttc taagtgttcc tttgttccat
    22081 aacctgcatt atgcgagaaa ccatatccag gataaagcac gtcatattct ttcatcaatt
    22141 ggtctcgata tactttagca atgatacttg cagcagcaat cgacacagat cgtgcatccc
    22201 ctttgattat tttttcctgc tgaataggca aatccaaggt catcgcatcg atcaacaaat
    22261 agcttggtac aatggacaat ttttctactg cttggatcat cgctaattta cttgcttcat
    22321 aaatattcac atcatcgatt gtctgctgat caacgatccc gatcccgatt gccagggctt
    22381 tttcttgaac ttgttgataa agaagttctc gctttttctc tgataattgt ttggagtcat
    22441 tcagccctaa tatgatctca tttttcggca agataactgc agctgccacg acaggtccag
    22501 ctaatggtcc tcttcctact tcatcaatgc cggcaatcca ttggtgacct tgtgcgtacc
    22561 cttgcttttc aaagcaaaac atttcttcat atttttcctt aattaatgct tcctttttca
    22621 atcgtcgttc aaaagaagcc agtgcttgct gcacgccttt gcgtggatca tttcttaatg
    22681 attctagtgt aggatcatct attctctgta tgcttttcaa tcgttctttg atactttgga
    22741 tggattcagt catcactttt ttgctccatt tcttcgcaac gatccagtgt gtatcgtcca
    22801 agttttcctt gacgaatatc atgcacgact aattcgctgc cacgatcata gtcttcttta
    22861 tacccacgtt ttgctgtaat atccatcagt aattccggtg ccaatttttc ttcttgctcg
    22921 attgataaac cataacgatc gctgattctt cctgaataat atcgggcaaa aaagtctaat
    22981 ccgtaaatcg ctaaatcatc taaatggaca agctggtctt tgatcgcgcc tgtcagcgct
    23041 agttttttgc cgatttcttc atcttcaaat ttgggccata aaatccctgg tgtgtctaac
    23101 aattccaact cattacctaa acgaagccac tgctgacctt ttgttacgcc aggcttattt
    23161 cctgtctgtg caattttctt gcctactagt cgattcatca acgtagattt cccaacattt
    23221 ggaataccta agaccattgc ccgaatagcg cgaggcttga tcccccgagc agcatctctt
    23281 gcccgttttt ctttcaatgc ttctttagcc gcaggtacta gacctttaat tcctttactt
    23341 tcttgtgccg tgatagctaa tgcaagatgg ccttgtttac gaaaatattc ttgccacagc
    23401 tttgtctgtt gtggatctgc cagatccgct ttattcaata aaacaaggcg aggtttttgc
    23461 tgaatgatct gatctagcat tggattacga gaagataaag gtaatcgtgc gtctaccaat
    23521 tcaaatacga tgtccacata tttcattttt tcagaaactt ctcgtcgggc ttttgccata
    23581 tgtcccggaa accattggat ggtcattgtt ctctccattc ctttcatacc ggaatattcc
    23641 ccggctttac ttgttttttt ctttttcaat ataaattcac ttttttatta taaagaaatt
    23701 ccatctatta gttaagtgaa agtgaaccag tcctcattct atcacatctt cacttttttg
    23761 atgatgcaaa tttttatttt ggtttctata tatgaatcta aatttttgac agcgtttcct
    23821 aaataagaga tgatagacga acaaaacaga aaggagacga aacaaatgaa tagtatggtt
    23881 tttgttaatt ttcctgttgc tgatgtcaat cgttccgttg ctttttatga aaagctgggt
    23941 tttaaaaaaa atgaagaatt ttctactgaa gaagctggtt caatggtatg ggatgacact
    24001 ttttggataa tgcttctctc tcatgatttt tataaaaaat ttataaaaga taaagaaata
    24061 gctgataata aaaaaacaag tggtgctttg atttcgttta gtgttgaaaa tgcggatgaa
    24121 gtaaaaaaat ttgctcaaat tgcaaaagaa aatggtggta ctttccacga agtagatatg
    24181 ggaatgcctg aagaagaaat gttttcatta gaagtccaag atctagacgg aaatacatta
    24241 gaacccgtat ggatgaagat gtaagcagat aactaatctg acgtttttta taacaaaaaa
    24301 acaaaggcgg atgatacgat aagtattctc cgcctctatt ttttttattc gtacaatctc
    24361 ttttttacag tttataaaag cagtcttctc accctttttt taattgttag atgccagtgt
    24421 atgtaaatca ccaatgtgtc atgacacatt ggtgatttta tggtatcctt ttattattaa
    24481 ctgacaaagg agagaatatt ttgaacactt tgattgcaaa agacgttttt agattgcccg
    24541 aaatgaatgc aagattgttg tcgacctctg ctcttttatt agcccttgat gtcatcttat
    24601 acaagatagc gattggtcct gcttattttc aaatcacatt cggattttta agtatggcgc
    24661 ttatgggtta tctatatgga ccgatctggg ctggtttgct tgaaatgttg tcgaacactt
    24721 taaatttcac gatatttggt tcaggaacgt ttcaagctgc ttttcttatc acggctttcc
    24781 ttggtggatt tattaatggc ttatttttat atcataaaaa gataaacctg ccttccgtca
    24841 tcgctgccca attattgatt atgatcccag tcagcctttt tatcaattct tggctgatgt
    24901 ctgttttgtt tggtgcagat ttcaaagtaa tttttctagg tagattcatc aggaatatga
    24961 tccaaatccc tattcaaata atggttgttt attcatttat caaaacttta gaggctagaa
    25021 aagtgtttaa acgagctgtt tgattttacg tactaaggta gtaaactgat tttggattaa
    25081 acaataaagc gtgcagtctc aacagactgc acgctttatt gtttctcact ggatccattt
    25141 tatttcatca agtggataat agacaaatct tgctttacct agaatatcag ttttattgat
    25201 cgtgccaaac gaacgactat cttttgaaac tcttcgatta tctccaagta caaaataatt
    25261 atcttccgga agcttggatt gtcccgttag ctcttgtagt gtaaagtccg tcgtatagga
    25321 tgcatttgca tgatctgatt tcaggttttt agttagataa ggttccttaa ctactttccc
    25381 attgatcttc aattgatcat tttggtagct gacattttct cctggaagac cgattactcg
    25441 tttgatataa atcgtgccat ctgctaattg aaaaacaaca atatcaaatc gtctgatctc
    25501 cgaaaatttt tccatcacaa ccatatcacc ttgcttcaac acgttttcca ttgaattgcc
    25561 ttccactggt accgggatga ataaaaatcc ccgcaacatg aacgcaagga atacagaaac
    25621 aagtaagtat ttgaatacaa gccagaagcg attaatatag cgttgttttt ttgtcaatct
    25681 ctatcaatcc atccctcttt ttttcattct ttctttatta taagaaaaat atcaaaaaaa
    25741 tgcgactaga tagctgaaat aaaaataatt ctttcaatta ggctattgga caaaaaactt
    25801 ttttgatcat aaagtacaaa actatctctt atatgtcatg attattttct aaatattttt
    25861 cccatgcttc gtcaaagata tccatggacg gcaaatagtc gacattcagt tccagataat
    25921 tagaaatttc ttcgtagcta gagctgtgct taggaaattg gatgtcttta ccggcttctg
    25981 tagcgaaaac agataaagca tcatgtgtgc caccgcctcg cagtgtcatt aaataatgaa
    26041 aaaagcttcg attcaccctt cgttcctcca attttcttcg ataaaggcag cccggcgtgc
    26101 atggtttctt ttaaaatttt caggatgctt tttataatac tcttgatggt cttcttctgc
    26161 tagataaaat ggttgggctg gttcgatttt tgtaacgatt ggctgttcga aacgtccact
    26221 ttcagctaac tgtttcttac ttttttcagc aatgatccgc tgttcttctg tcaaataaaa
    26281 gatcaccggg cgataattgt caccgcgatc ctcgaattgt ccgaaagcgt cagttggatc
    26341 agtttgctgc cagtaaagtt ctactaattg ttcatatgaa atcaatgttg gatcatagat
    26401 aatctccact gcttctgtat gtccagtcgt ttggcttttt acttgttcat aagtcggatc
    26461 tggtacatgc cctccagtat atcctgacat cactgtatgg ataccaggaa gcgtgtcgaa
    26521 aggctggatc atacaccaga agcagcctcc tgcaaatatt gctcgttcca ttatgattcc
    26581 ccctctaata ctactcatcc gatgattttt tattttcttt tgtcttgctt tcagtactgc
    26641 tggtgctagt tttgttggta tttgagttgt ttgaagcatc agaggtatct tttggcaaat
    26701 atagactaaa ccgaatgtca tcatttacta agtcgatttt atctgcttta atatacatcc
    26761 cgttttgcat tcggaattga tccaacctaa gcatgatagt cagatcatct gtattgactt
    26821 cgatccaatc tggtaattta tagtttctct tgatcatatt catcacatct ttgattggca
    26881 aactcaatgt tccgatagaa agacttctag cttttagttg gacatttccg ttttccatca
    26941 catacggatc aaaatataaa tagaaatcga taggaaaacc tagtacttcg aaagtaccat
    27001 tgagtagtgc ttcattttcc aaataaaatt tatattcaat atcactatct ttttgatact
    27061 cactaaggaa aaagctgatg atttgattga ctttttcttt atttgaattg atcgttaaga
    27121 caggatctcc ttgtctttcc gtaagagctg gtatttctgg gagttctggt tcccgattag
    27181 caaaaatacg gctgcctaaa aatacggtcg aaccaagtac taccccaaga aggataagga
    27241 aggcgaattt ccagtaattt gttttctttg ttttgtttga agatacgtca gatcttgttt
    27301 tcatgtttat tgatcatcct ccttctctaa ccattggttt tgtgtctcga tcagctgatc
    27361 tctcactgca tttgccatca attgataacc taaattattt ggatggaaat gatcttcttc
    27421 atagagcaca ttatttttga tgccatttag attttctgtc gtgctagttg tttgatctgt
    27481 tccttcttct gtgacaccta cttcctgatt caatccttga taaagcaaat cattgattgg
    27541 gataaagtag cagttcggca ttttttttgt caccgtttct gttcctttgt tccaattgtt
    27601 aacaattgtc tgcatgtcag tgatctcagg aaagttcaaa taaaatggat tgtagatgcc
    27661 taaaatgtaa ataggtgcgg ttttattgag ggatcgaaat tcttccaata gttctgtgac
    27721 attttcctga tagtttttca gtggcttttt aaaggaattg accgttaacc cgaataaatt
    27781 attttggata actttcatca aatcattacc gccaacagtc agtgtgataa aatcagctgt
    27841 tttgatattg tttcgaattt cttcttttgt ttttactcgc tttaagatct ggtcacttcg
    27901 ttcaccattt acaccatagt tttctgtttc aaccgcggtc aaattataac ggtcttttag
    27961 atcatttgcg acaaggggaa cgaatcctcc ttgcttggtt tgatctccta ctccttcagt
    28021 cagcgaatct ccgatagcta tgtagtgaag tttttctttt ttatttggat cttctctcac
    28081 agtagttact ggtttcatta gtgggccagc tttcggtata gaagcagcta aacctgcata
    28141 gatgatgatc gcaattaata atgggaacaa aacgaggatc aaatgtcgtt tcgttaattt
    28201 cattttttca cctcttgtat tcgattggtt ctaatgttat caaaaatttt atcaaaaagg
    28261 tagtctttat gaaaaaagaa aacaattatt ttcctaattt tataaagaaa aagagaataa
    28321 gctgcaagcc tttcaggctg agggcttatc ctcttttcga ctaatctaca taatacatga
    28381 ttgcaaacgc gtctttacca gtatgcgtag cgataaccgg attcgtatgc agtacaggga
    28441 tatgcatgtc cgggaataat tcttgcaaac cctctttgaa ttcttgcgcc aattcttttg
    28501 catccgcatg tgaaataccg atttgtcgca catttggcaa ttctttcagt tcatttttca
    28561 aattattaaa ccatttatcg aacgttttct ttcctcgccc cttagttaca gggattaatt
    28621 cagaatgggc gaaattcatc accacgcgta tgttcaaaac actagacagc aaaccagttg
    28681 ttcggctgat tcttccacct tttaccaagt tatctagtgt ggatattccg atgtataatt
    28741 ttgtattgtc ttgtactttt ttgacttctt caaggatttt ctctactgtt ttaccttctt
    28801 ttgctaattc agctgctcga attacctgaa aagataatcc ttggtcagtg gtatcactgt
    28861 cgataaccgt tactttcgtt tttgttagat tactggcttg tcttgctgct tctactgttc
    28921 cactcaagcc ttttgtcata tggatcgaaa tcacttcact gccgtcttct cctagctcat
    28981 catacatcga gatgaattct ccaattggtg gttggcttgt tttgggcaga ttttttgctt
    29041 tcgccatcat ttccataaat ctttcgccgt ctagttggtc atcatcggga tatactgttc
    29101 cgtcgatcat gatggatagc ggcatgacac gaatgcctaa agcatcgcgt acattttttt
    29161 ccatcgtaca tgaagaatct gttacgattt ttacatttgt cattattttc tctctctctt
    29221 tctttaacaa gatgccttat tcatgttaaa ctacatgtaa gacaatttct atttttagta
    29281 taacagacaa ataatgattt ttcattaatt acacctagta aaggagccgt tttcatggaa
    29341 aaaacgaaat ttaatcgaac gtatgaaata ttaaatgaag tgtttaatgc agtcacccat
    29401 ggaattggga ctggattgag tatcgcggga ctagtggttc ttttagtaaa aggtgcccac
    29461 ttgcattcct ccctccatat tgtctcttat gctatctatg gatcgatgat gattctttta
    29521 tttttaacgt ctactttgtt tcattcattg atttttacaa aagcaaaaaa ggtgttccaa
    29581 gtattcgatc atagttcgat ttttcttttg attgcaggaa gttatactcc tttttgtttg
    29641 ctgagtatcc gaggatggct aggttggact ttatttgtat taatctggtt gtttgcgatt
    29701 cttggcatta tatataaagc gttgacgttg cataaacggg agagagtatc aaaagtatct
    29761 acgatcatct atatttttat ggggtggctc tgtgtcgtgg ctgctgttcc tctttatcac
    29821 tctcttggaa tgactggtgt tattttgatg gtagcaggcg ggctttctta cacgataggc
    29881 gcattctttt atagcttgaa ttctatccgc tatatgcatg ttgtttggca tttgtttgtt
    29941 atgttaggtg caggttttat gtacttctct gttttattct ttacttaata aaaaaaataa
    30001 agctatggat gggacagctc taaataacag ctagtctatc catagctttt ttatatcgtc
    30061 aattcgcctg tcccgctttt tacaggattc tcattacgct gatacgtttc aaattgatgc
    30121 ggataacgat ttttttcatc catttctcct tgactagtat tgatcagtga ccaatcagac
    30181 cagtcaacag gtggaaaata agcatctcca tcgaatacat gatggatgac tgtacggtat
    30241 aatatcgtac aaaatggcag aaattcttga taaacagctg accctccagc gatgaaaaag
    30301 ataccgtctg tttctttttc cagagcaagc acgtcttcca cactatgcat gacatgcacg
    30361 ccttctgctt tataggaaga atctctcgtc aaaacaatcg tctgacgatt cggcaacgga
    30421 cggctaccca tcccttcgaa tgtttttcgt cccatcacta gtgtatttgc ttctgtcatt
    30481 tgtttgaaga atttcagatc atttggcaaa cgccaaggaa gacggtcttc ttttccaatc
    30541 aatccgtttt catcctgtgc ccaaattgca gctaacattt tctcatcctt tctgattcta
    30601 aacagcgata ggagctttga tcgccggatg tgggtcatat ccttctaatt ggatatcttc
    30661 catctcaaaa tcaaatactg actgcttttc ctgattcaaa actaaagtcg gaaatgcgcg
    30721 tatttctcgg ctcaattgtt ctttcatttg ttcgatatga ttgatataaa gatgagcatc
    30781 tcctaatgtg tgaacaaatt cgccaaccgc taaacctgtt tcatgtgcaa ttaggtgagt
    30841 cagcaacgca taacttgcaa tgttgaacgg aacgcctaga aacacatccc cactgcgttg
    30901 atacaattgg cagcttagcc gtccctcttg aacataaaat tgaaacatcg tatgacaagg
    30961 aggcaaagcc atgctaggaa catcttccgg attccatgca gaaacgatca atcttctaga
    31021 atcaggagtc tttttgattg cttcgatcac ttgtttgatt tgatcgataa agccaccgtc
    31081 ttttgtttcc cagtgacgcc attgatagcc gtagatgtgt ccaagatcgc cgtatgtttc
    31141 agcaaaggaa tcatcggtta ggatcttctc gcaaaaaagc ttgctctgct ttttatattc
    31201 ttcattgaat tcaggatctg tcaaacatct atggccaaaa tcagtcatat ctggtccatt
    31261 atagtctgga cttttgatgt agcgttcaaa tgcccattca tcccaaatat ggtttttatt
    31321 ttgtaataga tagcggatat tcgtatctcc ttttaaaaac caaagcaatt cgcttttgat
    31381 caatccaaaa ggtactcgtt tcgttgttaa caaaggaaac cctttttgca gatcaaaccg
    31441 catttggtat ccgaataagc ttaatgtgcc tgtgcctgtt ctgtcttctt tacgatgccc
    31501 ttcttccaac aattttcttc ctaagtcaag atacgcttgt tccatccttt ttcctcctaa
    31561 tcttctaccc attcactcaa gtattcccac cgtgccattt tttcttcaag ttgtgcttct
    31621 gtttcagata cgtcgttttg caattcttgt aatcgagtga aattatctcc ttgatgattc
    31681 atctcttctt ggagcaatga gattttttct tcaagttctg caatctcatc ttcaatcgtt
    31741 tcccattctt tttgttccat gtaagataat ttcttctttt cagcaggttc cttgctctgt
    31801 atttttggtt tcgctttttc agcaggctgc tgggtctctt tttgcttcag taaataactg
    31861 gacatgtttc cgtaataaag ttcttgtttt ccgtttcctt ggaatactaa taacttatct
    31921 gctaccttgt ccaagaaata acggtcatgg gaaacagtga tcacagcacc tggaaaactt
    31981 ctaaaataat cttccaaaat cgtcaatgtt gctatgtcca aatcattggt cggttcatcc
    32041 aataataaaa cattaggttg ctgaatcagc agtttcaaca aaaataaccg gcgtttttct
    32101 ccgcctgaaa gtttgcctat cagtgtccca tgcataaatc ttggaaataa aaatcgttca
    32161 agtaattctg ctactccgat ttgactgcca tctgctgttt ttgcttcttc agcagcttct
    32221 tgtaagtaag caatcatccg tttatctggt gccatttctt cattttcttg tgtataatac
    32281 gcaagatgaa ccgtttcacc gatagacatt gtaccgctat ccaggggaat tctgccagct
    32341 agaatattaa gcaatgtcga ttttcccgca ccatttttac cagtaatacc tagtctttcc
    32401 cgagattgaa tcaataaatc taaatgttct agcagtgttt ggtcatttat acggtaactg
    32461 ccatctttga tttccagtac ttttttccct aaacgttgtg tcgctaggtt cagctctaaa
    32521 tcatcttctt ggtttacttg gtacaagttc tcctttagat cttcgaaacg gttgatccgt
    32581 gcttgctgct tggtgccacg tgcctgtacg ccagccctca tccaagctaa ttcctgttta
    32641 taaagccgct tgcgcttttc ttcttgttct acttctacac gatcacgttc tgctttttct
    32701 aagacgtaag cttcataatt tcctttatac tcgtataatt ttccaaatga aagttcgaag
    32761 attcggtttg ctactctatc tagaaaataa cgatcgtggg taaccatcaa tagtgcgcca
    32821 cggtattgtt tcaaatagtt ttccaaccac tcgatcgctt cataatctaa atggtttgtt
    32881 ggttcgtcta gtaatagtaa atcgggttca tcaatcaaca cttgcgccag actaactcgt
    32941 tttttctgac cacctgataa agtactgatt tttttatcta attcactgat tcctaatttt
    33001 tgcaaaataa tctttgcgtt ggtatctgta gtccaagcat cttctttatt catttgttct
    33061 tctgctttag cataacgttt ttgcacatca gggtcttctc cgttttcccc tagttcgatc
    33121 aatgcttcct catatgctcg aacagtttgg atcaaggggc tgtttccttg aaagactgct
    33181 tggagaacag ttaattcatc ggaaaattca gctgcttgag aaagataacc aatacggtaa
    33241 tcagttggat aaaacactgt ttgccgatca ccgtcgctgc tatcgatacc ggcaataata
    33301 tttaagagac ttgttttacc cgtcccgttc gtcccaatca acccgatacg gtctttttcg
    33361 tggaccaaaa aagaaatctg gtcaaacaaa tctttttcac cataagtttt tttgaggtca
    33421 gttactttta attctttcaa catgttccct ccttatcttg gatattgtat cataaaaata
    33481 gtgaacttca ggaaaatttt tttggtattt gttctttttg tgttctcaaa aaaagaacag
    33541 gaccgattcg atcctgttct gatatgtttt gttttttata aaacttgaaa atttttacct
    33601 ttttatttta ttggactcgt tttttttgac tttgctcgaa acgcaaatcg gaaagaaaag
    33661 ttatcagatg gtttgttcat cgtataagat tctaacaaat tgatattcct gcgatagctg
    33721 ttcgcagaaa acgtcgtgat tttttcagca acttcgtatt catcaatgat ttttctttca
    33781 agataataac ctcttagcat ttttttcgta aacagactat cttgctggat catccacgaa
    33841 gaaatcaaat tccccgagtt catttttatt ccttcaagtt tgcgttgctg gataaaaaat
    33901 tgaaccatcg tttcatcata aatatcagtc aagttatcta aactttcaat aatcaactga
    33961 gtattttttt gatatgtctc tctgatttct tggatatctt catcactaat aaatgtctga
    34021 cgatcagcat atcttctttg ccaaaacatt ttcggatgga gaacgattcg cattaaccta
    34081 cggacaacaa tgaaccaaaa gccgataaaa gacaaaatct ggcttgtgat agaatgttcg
    34141 aatcgagaaa gaaaacggga gtaaaaacga taaccattag aagaaagctt gcccgaacga
    34201 taactttcat ccaatccatc ccgttcgatc gacaaaatca gtgcttgtat ttcttgtact
    34261 tcttgctttt catcatcggt taaatcttcc aagtacagat cacgtatccg ttcttgataa
    34321 ttttctatca ctgcttcagt ggctaaaaat tcttttgtat tacgatcaat ttcctttatt
    34381 tcttttcgca aaacttcaac gacctcttct aaaatcatca aagcattcaa atccgtgctt
    34441 ttcgcttcct ctgtttccgt cagcataggc aagacaatga tccctatgac caaagtcact
    34501 aagatgacac ccgccgtcag aaacaaaagc aaagaccgct ggtaaaacat catattattg
    34561 attgaaaatg gcaaaataaa aatcgtagcc aaactgactg ttccctttac accgccaaat
    34621 gttaagatca agatctcgtt caactgtttt ttgaactttt tactgccatc tttgaataca
    34681 taaaaaagac tgatagaaat gaatctgatc acaaaaagca tgacagagat taatacgata
    34741 atcgctaata aaagaccgtt tggatataaa ccgtctcccc atactggcga gaacacttgc
    34801 gtcaattcga ttcctaaaaa tataaatact aatgcattca gtgtaaaagc aatagttgtc
    34861 catgtactgt gggacaagct cgacaattct gcatcaaaca cagagatttt cctaaaacca
    34921 gaagcttgga gaatacctgc tgccactgca gcaatgattc cagatacatc aaatgcctct
    34981 gccaatacat aggctagaaa aggaagcaaa agttcgatca gcagataccc tgtgacatct
    35041 tgtgctgatg ctttttcgat caaatgaatt attttttgtt tgaaccagac aagcaaaaag
    35101 ccgatcaaag ctcccccaat actagaaact actagactca tccccgcgtt tactgctgaa
    35161 aaagatccgg tgatcagcgc accaagagca aactggaagg cagtaacacc agatgcatca
    35221 ttcaaaagac cttctccttc caaaatatgc atcgcctttt tagggatatt cactcgtcca
    35281 gaaagagaac tgactgctac cgcatctgtc ggacctaacg ctgctccaaa agcaaagcag
    35341 gcagcaagtg ggacacttgg caacaggaat gataacgtcg cacctacccc gacaagggtc
    35401 aaaatgactc ctccaaatgc caagaacaaa attgtcccga aatttttgag aatcgaagaa
    35461 atatctgctt tttctccttc tctaaataac aatggtgcaa taatcataac taagaacaat
    35521 tcaggttcaa aatcgattga ttgtccccac tctgtcagac ctaataccac tccaaggaaa
    35581 atttggatca gtggagctgg aatcattggc aaaatgcggc tagctatgtt cgataaggtg
    35641 atcgttactg caaatacaat aactaactca gcaaattcca tcgtccatcc cctttatttt
    35701 ttattttgtg taagcatttt taaaaattgt tcttttttct ttaaaatttt ggtttcgact
    35761 acttcaacct gtgtttggtt cccctctaat tcacactgta cacgttttga ctccaaacga
    35821 agcagatcat gtttcagacg ttccaactga cgatacatca atatatttct tttacgcaac
    35881 ttacaaagta tttgacggcg gtcatccatc gaaagggcgc catagcgatc atgcaacagt
    35941 tttttttgtt gttcaaaact ctctctcatt ttccagatct cctatacaca aacttccatc
    36001 attatagcaa agttcttttt ggatgaaaaa tatttcattg tttcatcgtt tattgctagt
    36061 tagaaatttc cataaatttt tcaacgcctc attctattcc aaatagtatc agatatcaag
    36121 taaaacttaa gaaagataaa agtttctgac aaaaggatgt ttactttccc attcctgcct
    36181 ttcttgttct tttatcacaa caaaaaaatg gggctgcgac aaaaatcttg tcacagtccc
    36241 ttattctgaa taaacggtgt ggaagagaca gcctatcagt agtaataaga cacatctctt
    36301 tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa aattccccgt atacatcttc
    36361 tacacgttga tctggattga tcacgatata caagttacct gttttttcgc tgatttttgt
    36421 tgattgatat tgattgtcta atccttctgt tcctttatct agatatggga agtacacaag
    36481 atcaggtgtg ccatttcggt aaaggatttc cattcgctcg atatcttcgt atttcaatgc
    36541 gcgattaaac atgcccattt ccagtccgcc taggttgata tcatgtgtcg caactgcgtc
    36601 cccttcatgg aagatttcga ttttaaatcc agcacacgga tggatttcaa caaattcact
    36661 gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc caaagatcgc cgatatgacg
    36721 gcggttgatc gtccaggttt ctccatttct aaaataaaat tttagcgctg tcatttcttt
    36781 actcattaaa aagactcctt tatgatgtaa tttattcttg tactacctca gtttagcaca
    36841 aatttatgta agtggtcaca tcttatgttt tttcttctaa tgacgtatct gcccacttta
    36901 agtagccaac aaagctgagc agatacagtc tagtgttttt taaatcaatt tttctttcga
    36961 taaaaaatcc aaaacctgtt ttttatcatt ttttattctt tgttctacga cggctttttc
    37021 agctaattgc aaagcttcac taacccactt accaggtttt tttccagttt cttctagcaa
    37081 gattttacca tctactgcta attcgcttaa tgacttgata ggtaaacgat cataggattc
    37141 tcctacggca tccatctgag gtgtttcgtc aaaatagatc atcaattctt ctacttgcag
    37201 tgcatgtttt ttgcctaaga agtacaattc gaatggttcc aaagctccag tttctcgttg
    37261 tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt tggttagaac acttccacga
    37321 tttcaaaaag gaacgaatct cattttcttt tagatccagt tggctgatca ataatgccca
    37381 tgcttggctc tcttctttca atgggacgga tggtaattca gcaaaacgta aaagtgcttc
    37441 gccttcttct ttcaaaccgg gacagcaaat gtagcactct gtttcaataa atgcatggat
    37501 tcccgcggcc cgttcatttc ccaagagcat cttgatgaat tccacattta tacgttctac
    37561 agatatttta cctaatagct cgtggttttc tttgatggcg tagaaggttt cttcctctaa
    37621 tttgaaccct agttgactga caaaacgcaa cccgcgcatc attcgtaagg catcttcatg
    37681 gaaccgctca tgaggattgc ctacagctcg caagatctgt tcttctagat cttgcagacc
    37741 atcgaacaaa tcgatgattt ctccatcttc tttcaaagca aacgcattga tcgtaaaatc
    37801 tcttcttttc aaatcttctg ccaacgaacg aacgaattcg acatgatctg gtctacggta
    37861 gtcctgataa gttgattccg tccgaaatgt ggtgatctca tattgttcat cattttctaa
    37921 taccaacaca gttccatgtt cgatcccaat atctatcgtt tttggaaaaa gctgtttgat
    37981 ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg attggctggt tcagcaagat
    38041 atcacgaaca cttccaccta caaaataagc ttcatatcct gcattctcta attttttcag
    38101 aactggaatt gccttttggt attccagagg taattgtttt aatttcattt ttccaacctt
    38161 tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca tggtgactgt ttgacaagtc
    38221 cgtttcagag tcttcaaggc caggaccact tcatcttgtt tactcgtcct tacgctcaaa
    38281 gcgattatgg tctctttgat tgaatttttc catgtttttt tcaaaaactt cagttaagtc
    38341 gatattcaat gaattagcca tgatcatcgt aacgaataaa acatcaccaa gttcttctgc
    38401 taccgagtta actggttcag aagccttttt gcttttttct ccataagtgt ggttgacttc
    38461 tcttgccaat tcacctactt cttcagttaa tcgagccatc tgagctaatg gagagaaata
    38521 ccccaccttg aattgctgga tatattcatc cacttcctgc tgcatactgt ataaagaacg
    38581 ctttttatcc atctttttcc ctccaaaact atcttaccaa aactttaacg caaatcctag
    38641 ataagagcaa ttgagaaaat cgaatttcca tgctacattt aggaaggaat ttctaaatgg
    38701 aaattctgtg aaaatacata attgaaagct ggaattcata tggtcaaacg aaaaacagta
    38761 aaagaagttc tttttatcat tataggaaca agtatttatg catttggtct tgtttacttg
    38821 aacatcgcta atcatttagc agaaggggga gtctcaggga tcaccttgat cttaagagct
    38881 ttatttggta ttgatccagc ttatacgact ttattaatca acatcccatt gattttacta
    38941 ggaggaaaga tcctcggtaa acgttccttg gcctatactg ttctaggtac actttctttg
    39001 tcattctttt tatggatctg gcaaagggtt ccactagaaa tcaatttaca gcatgatcta
    39061 ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag gaagcgggat cgtctaccgc
    39121 atgggtggaa caacaggcgg cagtgatatt atcgctcgga ttttagaaca aaattacggc
    39181 atcactatgg ggcgttccct attggcattt gatatcatcg tcttacttgc ttcattaacc
    39241 tatatcgact tgaaacgtat gatgtatacg ttgattgttt cgtatgtttt cagtcgtgtg
    39301 attgattcga tcttagatgg cggatattct gcaaaaggtc tacttgtcgt ctccaacaaa
    39361 agcgaagaga ttgcaccact actgatgacc ggtctgcaaa gaggtgtgac tttcctttct
    39421 ggagaaggcg cattctctgg taattcaaaa aaaatcatct atatggtcgt tagctctagt
    39481 gaattaactg aagctaagag gatcgtccac gagattgacg aaaaagcttt cctttcaatc
    39541 atcaatgtgc atgaagtgga agggcaaggc tttacttatt tgaagccaca gacaagatta
    39601 ctgaaaaaac tataagaaaa aagacaaaga taggctatga ggaaagattc cacatccagc
    39661 tgacctttgt cttttttagt actatactat ttttaactaa ttgttatgtt cacttacccg
    39721 gttacttttc ttttgtgtct gcaaaaggat ctttcggtta acttcatcag ataatttcaa
    39781 aaaagcatcc ctatctccat cttctagtgc ttgatcgatt tgagcataaa gcatttttag
    39841 ttttgcctct tcttcttttt cttgaaaata tcgttcaatt aattcagtat cttcttcact
    39901 gactgaagaa tcccaagaag aaaatggatt gtcttctaag atagatagat attctcttgt
    39961 ctgccaagca ttttcgaata tacattccac atataaaggc tttttccaat tcaaacggat
    40021 ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc tgcaaaaaca atttcatggg
    40081 ttccccttca acagatagat cactgatctg cagtcctcga tccgcaaaat tcgccccttc
    40141 aacaaagtgg acattgctta aaatcgcttc atgattggca agataattca agatccaaaa
    40201 aacttctctt cggctaaaag atacatgatt gaccatccac gttaaaaaat tcttcttttc
    40261 ccgtacatca attagcattt ttctcacctc tctgttaggt catctaataa tgacagtgcc
    40321 tccatatctc ctggttcaag tgttaaataa taagtcaata attcttgcgc tcttttcagc
    40381 tgcccttctt ctcttaagaa aaaggcatac tctttcaaga aatctggttc atgtgatagt
    40441 tcatgataag cctgttcata gtgtacagct gcaacggtaa agtcttctaa ttcattataa
    40501 gcatgggcta ggttccattc tgcataagga ttagcagttt cttccatctg attgatcact
    40561 tgtatcacat cttcatatct ttcatcatcc aagtaaagat tacttaaagt aagtaatgtt
    40621 tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa gttcttctgc tcgctgtcta
    40681 tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa gttcgacttg gtaaggattt
    40741 tctttgattc cttcttctaa cactgtttgt gcttcttcaa tcatttcttc ttcttgaaga
    40801 gcttgtccta gatatagata taacgattga taatgcggat taacttctcg aagttcttcc
    40861 agatagttga ttgcctgctg attttctttc aattgaagat tcgtaaaagc tagctggaac
    40921 agtcgatcat ctgtcctttc ttctttcagt gcttcttcta aaggtacgat agctgcttcg
    40981 aattctcctt gcatacttaa ggattgtcct aaacgttcct gcatcgagat tcctgaaatt
    41041 tcatttatcc cgcttgccaa caatgtttca taaaccactg ctgcctcttt gaaacgatca
    41101 actgaaaaat acaattcagc tagcgcaaac tggatcaatg gttcatcagg aagcaaccca
    41161 gctgcttctt tcaattttgc ttcactcact tcagggattc cgataacttg gtacaaatca
    41221 gctatcgcca ataagctttg cggatagtaa tcactttgct tatccacttt ttccaggtat
    41281 tcaaaagcta agtcgatctc attatcttcg attgcaattt ctgctaacgg gatgttcagt
    41341 ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa tctgttttgc ttcgatcaaa
    41401 aagccaatag ccagcaattc ttcgcctagt tcagctaata tatctgctgg gtcttctttt
    41461 aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt catctgataa agcttgcaac
    41521 attttttcgc tatttgtatt catttcaatt ccctcttatc tcactgacga tttgttgaaa
    41581 taccgtcatt gttctcaata attctttttc gtttggatgt tggtatccgt ctattatacc
    41641 tattttttct aaaacaagta tttttttgct tttttctgcc aaaaaaagaa ctttttccaa
    41701 atactccatc tttgaaatct gttccagcca gtggattgga tagccaagca agtccagcca
    41761 cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct tggagattcc acacgaaatg
    41821 aaacatcatt cctaaaaatt ttttcatact agcagaaagt agatggccat tttcggtcag
    41881 ataaaaagct tgttcaaaca gtttaccata atttgacagg ttttctgcct ctaattgata
    41941 aaaatagatc atttcttcga tcatcccggc aaacggccga gtcgttaatt gctttttatt
    42001 tggatagttt ttatacagat tttgcaaaaa acgatgatca caaatcattc cgcaacagat
    42061 aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc tcgctgattg tttgatcata
    42121 aacgatgcgt tctggcagat tgatggtttg cagcactaca cgatttggta gctgcaaaat
    42181 cgtccgctgc ggtgttaatg ctcggcttag agaacggatc gatacaggga tcatccatag
    42241 tgatgaatca agggttgtat gtttttgaaa aaaacctgcc agatccatga tcccttcatt
    42301 tccaaatccg atgataaaca tggattgatt ttcgggatag cgtttgacga aatcaagcag
    42361 gttctttaat tctgttaaat gattgcattg agtgttggca caaatatacc agtcattgtt
    42421 tgcttgattt gcaaataacg ggttgatctt atctgcaaac aaatcataat agcgctgatt
    42481 cgttaaaaat aagattggct gatcaatgga aagttctctt agctgactga ctagcgtttc
    42541 tccgtaaaac actttcgtca acgcatcttc tacttgataa tcaagttcca tacgcatccc
    42601 tcctcttctt atttttattt taccattagt aagcaaattc tagaaaagat aagagcaaag
    42661 ctcacctcat aacttgcaaa taaataaaaa ttaaataaaa tacatgtgtt ttctatgtat
    42721 gacatacgga ttcggaatct ctgtctctta tttattcctt ttctgctagg aaataaaaag
    42781 aaaagcactt gtagccctag agccacaagc gctttttcaa ctgtcttatt taacagcgtc
    42841 ttttaatgct ttacctggtt tgaatgcagg tactttgcta gctggaattt cgatttcttc
    42901 accagtttgt gggttacgac ctttgcgagc tgcgcgttcg cgaacttcaa agttaccaaa
    42961 accgattaat tgaacttttt cgccgttagc taaagcatct tgaatagttg aaaatacagc
    43021 atctactgct gcagttgcat cttttttagt taaatcagta gctgaagcaa ctttttcgat
    43081 taattctgct ttgtttgcca tggatagatt cacctcctcc acaagagata tgatagaaat
    43141 atcatttagt taaaactttc tgagtggtcc agaagtttaa aaagaaatat tgattaactt
    43201 caatattctg ttatcacgat atcatatgaa ccgcttaata gcaaggtttt taccaatttt
    43261 cacatgcttt ttttagtcat agaaacgtaa caaacgaaac tgtctttttc ttttgaaaaa
    43321 aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag ggataattcg gatggacgtc
    43381 ccttcaaaag taaatgcttt tctgatttga ttttctaaga aacgttcata agaaaaatgc
    43441 atcaattcct cttcattgac aaaaacgaca aatgtaggtg gtttgacagc gacttgtgta
    43501 gcataaaaaa tcttcaggcg ttttccttta tctgttggtg ttggattgat tgctacagca
    43561 tccataatga tatcattcaa cacagccgaa gagatacgaa gattctggtt catacttact
    43621 tgttcgatca tttcaggcag acgttccaac cgttgtttag tcactgcaga aacaaaaacg
    43681 attggagcgt aatcaagata ccggaattca tcacgaatct cttgttcgaa atcacgcatt
    43741 gtattggttt ctttctttac aagatcccat ttattcacga cgataataat tccacgacca
    43801 gcttcatggg cgtaacccgc tactcgcttg tcttgttccc gaatcccttc ttcagcattc
    43861 agtaccatca aaacgatatc agaacggtca atggcacgca ttgcacgcat cacgctgtat
    43921 ttttcagtat tttcgtatac ttttccgcgt ttacgcatac cagctgtatc tatcattaag
    43981 aatttctgac cactttcaga aacgaagtgg gtgtcgattg cgtctcgggt cgttccttca
    44041 atatccgaaa caattacacg ttcttctcct aaaatcgcat tgatcaaaga ggatttacca
    44101 acatttgggc gaccaatcaa actaaactta atcgtgtcgt cgtcctcttc ttcagtctct
    44161 tcagagaaat acttcactgc ttcatctaaa atatcaccaa tccctagtcc atggcttcct
    44221 gaaacaggat aaggatcgcc tagccctaag gaataaaatt catagatatc gtttctcatt
    44281 tctggattat ctactttatt gactgctaaa atcactggtt tattacttct gtataaaatt
    44341 ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc cgctgactac acaaatgatt
    44401 acatctgctt cttcaatcgc gatttctgct tgatgcttga tctgatccat aaaaggttca
    44461 tcgccaagat caattccgcc tgtgtcgatc acgctgaatt ctcgacctaa ccattcacct
    44521 tttgcataaa tacgatcacg agttactcca ggagtatctt cgacaatcga gatacgctca
    44581 ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg ggcggccaac gattgcaatt
    44641 gttggatttg ccatattatt tcctcctcac aagtttccaa tctcacttag tttaacgaat
    44701 gcattgccaa ctttcaagca tagattgtca attaaacgaa gtatttttat tccttattga
    44761 aatactaatc gcttttcaaa agaaaaactg agacaaaagc agctgctttc atctcagtca
    44821 cagatacaaa atcaattatt tttcagaatc atcggttgct ttttcttcta cttcttcttt
    44881 caacgcatca cctaaaatgt ctcccatagt aaatccagta ttttcttctg gtaattcata
    44941 agactcaaca ttttttggtt cttcttctga agctggtttt tcttctaaag ctttgatact
    45001 caatgcaata cgatgttctt ctggatggat ctctaatact ttcactttca cttcgtcacc
    45061 ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt gaaatttgag agatatggac
    45121 tagcccttct actccaggga acacttcaac aaaggcaccg aagcttgtca aacgtttaac
    45181 tgttccgtct aatactgtgc caactggagc ttttgtatcg atttctgacc aaggtccagg
    45241 ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca ggattgatag acaacacttt
    45301 gacatttaca tcatcattga cagccaatac atcacttggt tttgctacat gactatgtga
    45361 aatctctgag acatggacta gaccgtcaat tccgcctagg tcgacaaatg cgccaaaatc
    45421 agtaagacgt gcaacacgtc cactgagaac atctccctca tggatagttg aaagaatttc
    45481 ttttttcttt tcttctcttt cgttttccac taccgcttta tgcgataaga tcaaacgatt
    45541 ttctgatggt tcgatttcga tgattttaaa gctcaatgtt ttgcctttat agtctgaaaa
    45601 gtctgcaaca aaatgatctt ccaccattga tgcagggaca aaaccacgta caccgacgtc
    45661 aacgactaaa ccgcctttga caacatttgt aacaggtgct tcgatgacct tgccctcttg
    45721 gaaatctctt tcgatatctt cccaaacttt tttagcatct aaacggcgtt ttgaaagtag
    45781 atagctgccg ttttctttat ctttgccgat cgtactgata acgactaaat ctaatacatc
    45841 accgactttt actacttcgc tgatatcttc taccggcaaa gttgataatt cttttgccgg
    45901 aacgactcct tcaactccag ccccttcgat tccgacaatc acttgtttgt cttcaatgac
    45961 taaaacttcg cccttaacaa catcgccaac ttttacttct tgtacgcttt tcattgcgtc
    46021 ttccattgac tggttgttct cttgattttg atcaaattct gtcataacac ttgtcctcct
    46081 aaccaacatt ctgccgtaag tacggtactg tttttagttt aattgattat tcaaaaaaaa
    46141 taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt tttcattttt tttcttaatt
    46201 taaaaaagaa gataaccttt tttcaaaaca acagctttta ttgctgcaac gacttctgga
    46261 atcgatttgc cagtcgtatc gatacgaaca gcatcctctg cttgcttcag cggagagacg
    46321 tcccttgttg aatcataatg gtctctttcc tgtatctctt tcgtcagtgt ttcaagatca
    46381 gtcggaattc ctttttcttg gttctctttg aaacgtcttt ctgcacgctc acttacgctt
    46441 gctactaaaa agattttcac ttctgcattc ggtaatacag ctgtcccgat gtctcttccg
    46501 tccatgacaa cgccaccgcg ttcccctatt tttcgttgga cagcaactaa ttcttctctg
    46561 acttttccat gtgcagagac ttcagaaaca gcttttgtga catctggttg acggatttct
    46621 aaagtcacat cttttgatgc tatgaataca tgctgtccat cttcttgttg cgaaaaagag
    46681 ataggatact ttttgatcag cgaaactagc ccttcttcat ctgaaaaaga tacattgttc
    46741 gtaatagcta aataagttac tgcgcgatac atggcaccgg tatctgtata aatataattc
    46801 aattcttttg ctaatatctt ggcaacggta cttttcccag atgaagcagg cccgtcgatt
    46861 gcaatactga tttgtgtcat gatataactc ctttttctca gaaaaaaagc cgcaaagcgc
    46921 ggctattatt atttaattct taattcttga ccagggtaaa gcataaagtt gtttggatct
    46981 aagccattca attcaaacaa ttgatcagtg gtgatccctg cacgctcagc cacttgtttt
    47041 ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg cagctgatga agaaggtgtt
    47101 gtttctgctg cattgctgct tgaatcccct gcttgataag ttgcctcact tgaggaagtc
    47161 ggtgttgtct ctgctgcaat actgctttct gacgttacac ctgtatcttg agacacagta
    47221 gattcttccg cgattgtact agattctact gttgaagtct cagttgactc ctcttttgtt
    47281 gtacttgtag aactttctac tactgatgtc gaagtttcag aagaagttgc tgctgttgca
    47341 ttgttgcttg atccatttct gatccagaaa taagcacctg ctggaatagc gatacataag
    47401 accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc ctttcttttg ttgtctctga
    47461 gaacttcttg atgaaacttc atcatctgta tcgtagattg gttgttccca tggttcttgt
    47521 gtttcgttat tttctgtaga ttgatgtcta tcttttctgc tcactgtttt acctcctaaa
    47581 ctctatcgtg tgtattatac cataggaaaa agtgtcttgt cctaaaaaat caatgttcca
    47641 tgtttttttt aaataaattt agtaaaatcg tttcccaatg aagttgttca gtcttagaaa
    47701 aaagcttcct ctttggcaga agttctgctt ttgctccatc aatatcacaa caattctgcg
    47761 gcttttcgga gagtttttcg ccaaaataag ctaagatgaa ttttctgcgg cagttttctt
    47821 cattaatata gcgaagcatc tgctgcaggc ggaattcttt ttctttttcc tgacgtttga
    47881 gccgttccac ccaattttct ggtttttcag actgctggat cagctccatc catttttttt
    47941 gcagctcatc aaagggagct tgctctgtgg catattccaa ataaagctca aaactttgtc
    48001 gctgttctct agaaagttga ttaaagaaat aatggatacg ttcatcccca ctttgataaa
    48061 aaagaattgc tgcactttct tcctgatctc tgcctgctcg accgatttct tgtacatagt
    48121 tttctagact gtctggcaaa tcataatgga ccacataacg gatatcagat ttatcgatcc
    48181 ccataccaaa tgcattcgta gcaatgagaa actgcagctg attttttaca aactgctgtt
    48241 gaagttgtct tctttgagct gcatcaagac caccgtgata gtatccaact gtaaaacgtt
    48301 ccctgaataa atgatacaat cgttcgactt ctttttttgt tgcacaataa atgattgccg
    48361 ctccttgtgc tctttccatg aagtgttcaa gttcttgttc tttttgctgc gttttccgaa
    48421 caaaaagcga gatattttgc cgattgactg attgtctgat ctcttttggt ggtcgatcaa
    48481 atagtacatg acgtatatct cttgctacta aatctgttgc tgtcgccgtt agtgccagtg
    48541 tgaccgggtt tcctaaaagc tttcggattt ttcctaactg ttgatattct ggacggaaat
    48601 caactcccca ttgagacacg caatgagcct catctactac atataacgca atattttgtt
    48661 ttttcagatg ctctaaaaga gaaggattag tcaacatttc gggactcaaa aacaaaaatt
    48721 tatattgcga tagatgacgg aggacgtatc ttctttcaga ttgcgaaagt aaactattga
    48781 aagcagctac tcgtttctcc cctcgttttt gtaattgcgt cacttgatct tccattagag
    48841 aaagcagcgg tgacacgatg atgaccagac cttccattaa ataccccgtc aattgatagc
    48901 aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt atcttctcca tcaagcaacg
    48961 cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa tccaaaatat ttttttaatt
    49021 catttattaa cattctgtat cgcttccttt ctcaatagat agatttgata caaacgaaat
    49081 tctccataat ctaattgtcc atctacattc cattcgctaa agcgccactc tcgcacggaa
    49141 tccttatttg gttccaaacg ttcgattgtt tctgcagaga gaatccactc gtatggaaaa
    49201 tcatcaaaaa acaaggccca ctcaataaga tgatctgtta ctgttcctct ttttaaatga
    49261 cggattgcca tgatctggtc aattgtctga ccattcataa acatctgctt ggtgatcatc
    49321 atgctttgat tgaaattttg ctgtaataat gaatcgatca aagcgtgcca aagcgaatct
    49381 ggccgttttt cgatttgtgc taacaaaagg tggatacatc tgctttgttg cagataaacc
    49441 gctgactcat cattgttttt tgccagctga taaggaagca gccctgttcg ctcatttcca
    49501 gataattggt tagctaaaaa atctgctgct tcttctggta tctccgaaaa cagctgcacc
    49561 aaactctcat acgcagtatc aatcaaaatt tcacgtggca attggctttc actaagccat
    49621 ttttttagtt ggaaagtata aaaaggactt gtctctgctg gaagatagtc ttgaataccc
    49681 gttgccagat tagaaattac ttgaacagca aatttaatga gccgccaact agtttcccct
    49741 gttctgccat agcggtcaaa acgcaaacca gtatgttcta tttgatgttc ggacaacata
    49801 tcagcccctg caggcgttag ctttgcttca ttttcattta tttcgatcca accttgctgc
    49861 gcaatttttt gcaaaatttg ataaaactta tcctgcttca attctggaaa actgttatgg
    49921 ataaacaaaa gttcgtgggt aaatccataa ataagtactg aagtcgttcg ttttcccaca
    49981 aggagttggt aaagagaact aatccgcagc ttgtgtcctg atgaaaatag cgataaaata
    50041 aaaacaatca tattattctc cttaagttag gatggttaga aatgtcacta ctatgtaaac
    50101 ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat cgaagcgcca gatattttcg
    50161 attatgatga agaaggaatc gttttatttg cccaagaacc agaggctcat caacagttcg
    50221 tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca aaaatgtcca gttcgagcga
    50281 ttttattaga aaataaaata taaatataaa cgaaacttta aaaacgtaca ctaagttctc
    50341 taaacttgaa aataagccgg gatctgtttc tcttgttccg tgaaatcccg acttattttg
    50401 aaagattcat ttgctatcta tagttaattg actagttttt tcacggaccc tataggaccc
    50461 atttatatta tttcaacttt ttcagccagt tttccaatgt attgacgatc acagtttgct
    50521 ggtctgcgtt agaaatttct gcctctccat ctcctttttg ctcaccatag ctgccaaaac
    50581 ctccatgatt tccgccattg attgatacat actctgttct ttccggtaaa tactgcttag
    50641 cttcttgata agcttcctga ttcaatactt tatcttttgt cgccgtgatc gataaaacag
    50701 gaacagctga ttctgtcaat gttccttttt tgtcgggata acttgccatg aagaataccc
    50761 cttttaaatg tttgtctgaa tgattcttat tgacataacg acttgccatt acgccaccta
    50821 atgaatggcc accgatcaca taatcggaat ttggaaaatc ttctttgacc ttatccgctt
    50881 tgtttccgcc catcactgct agatcaagtg gcattttgac aatagcgact gaaaagccgt
    50941 tttttgctaa atcttgcgcc caaatactat aactttctgg ctctacgaga gcaccaggat
    51001 aaaaaataag taatggagac tttccatgat tctttttctc tccttcaaaa aataagtaat
    51061 cttctttgtt ttttccttct tttaccaaca cttgcgcttc tgacgatggt tcgtacgtat
    51121 tttgctgtat atagtaataa ccggctccac cgagtaatcc taaaatgatg actaagccaa
    51181 ttagtattcg tctaaatatt ttcactttct cccacctttt ctatctgagt gacccctttt
    51241 tcttaatcaa gtatgattat accttaaaaa aataagagag aaatgcaaac tttccagcat
    51301 caaataaaaa aagacggaat atccatttga atatccgccc tttttgtatg tttattttta
    51361 tttagcacat aaacgataca cagcttctgc ataaatttcc attgctttat acatactact
    51421 taattcccat cgttcattcg cctggtgcat aaagtcagga gtatcaggga acatcgcacc
    51481 gaatgccaca cactggttca tcgtacgagc aaaagtagcg ccgcctgata caaacggttc
    51541 agtcatgtct ccagtaatat cgcgatatgt ccccaataga tttttcacta actcactgtc
    51601 tagtggcacg tataatgaat caaggaagtc aaattcttca taagtcaacc catatttttc
    51661 tgctgtttta gttaatttag ctactaaatc atctttcttg aatgtaacag ggattctcat
    51721 atctacaccg atttttgttt gttctggtgt gatttccaaa cttgcaaagt tcatcgttag
    51781 ctcgccggat tgttcatctt ctgttttgcc cacgacagct tcaccagttg cattttcttg
    51841 aactaatttt cctaagaaat tgattgggcc aaaatcaaat acttctgaaa gagcaattgc
    51901 cagacgagaa atagcattca ctccttgtgc tgcatctttc gcgtggatac ttttaccaag
    51961 aacgacgatg ccttcacctt gttcttcgaa atcgaattcg tgttttttca atgcttcttt
    52021 tacttcagaa agcttttctc cagagtaagg agctgcatca ggaacaacat tcaacgctcc
    52081 tccagctttt acagaaaact cgttcgttcc tggtcctgtc aagtaagctt gcaataagcc
    52141 tttttctgca taaattaaag gaaattcagc atctggcgca aatccttgcg taattccttc
    52201 ttctttttcg ttatattttt ctaaacaacg ccataaattt tcttcatctg taccaaagat
    52261 aaaacgaata cgtgtcttga attccacgcc agcatcaatc aaagctttga cggcatacat
    52321 tgccgcaata gaaggacctt tgtcatcttg ggatccgcga ccaactaacc agccgtcttt
    52381 tatagttgga tcgaatggtt ttgtttccca attattttcg tcccctgctg gaacaacgtc
    52441 catatgacac aagataccga ataattcatc tccagagcca atctctgaat aaccgtaata
    52501 gccttctgga tctttaaatg ttctaaatcc tagcttttca ctgatttcca atactttatc
    52561 caaagcttca atcacttttt taccgaaggg atgtccttgt cctgaatctg cttcatctag
    52621 tacggaagga attcggatca attcgctcaa tgattctaat gcttgttcat gatgttgttc
    52681 tgtcacaaat tttttcatga tattatcctc ctaatatgtt aaaaagggtc atgtcaaatt
    52741 cctccgacac aacctcttct tctcaaataa tgacgtttta agcctgctcc atcagcaagc
    52801 agaatgttct taacaaatga tgaaagaaaa aacactcact gaatggagct attgacttat
    52861 ccacgtgctt atcgatagcc gtttatcggt ttcttcattc ttatgataga ccttgtacct
    52921 gtatttgtct aggtttagaa cattgctgct acacctagga tcacacagat ggctacaaat
    52981 aatatcgcaa ttaatttacc ggtaaatttc caccacactg aaacatcgaa acgagcaatt
    53041 gctacagcac ccattacgat agcagatgtt ggtgtaatta agtttactaa accagatgca
    53101 gattgataag cagtgattac taaatcctta ccgacgccgg caaattcacc cataggtccc
    53161 atgattccca ttgttgcagc agctaagccg gaagttgaag gaatcaagaa tgacataggg
    53221 atatagaaaa taaatgtaat aatgatgaat acacttgaag ataatgaact taatccttgt
    53281 tcgccccaat gtaagatggt gtctgtgatt aatccattgt tcataaccac ttggatacca
    53341 cgagcaacag ctactacgat tgctacaccg atcaaatcag aagcaccagc taaaaattca
    53401 gagacaaatt cagactcttt catgccataa gtgattgcta ctacgatgga cattactaag
    53461 aataacatag taatttctgg gaagtaccag tcacctaatg gaagcatgct tttacctaga
    53521 accgtaccta gtaccggaag gtttgttaac cagtttgtca atgattcaaa aatcgtgaaa
    53581 ttcgcattca aggttgtcca aggaatcaaa ctgatgatca tgataccaaa agtaataaag
    53641 aacaatacat taacatgttt ttgttttttc gtgattactt cttgacgacc gattgcttca
    53701 atatcgaaat gcttcatatc ttctttacgt tggttgtata ctaatgattt tgttggatct
    53761 ttttcgatct ttgatgcata acgatacaca tatacaatag aaatagctaa caaaataacg
    53821 aacatcaaga atcgaagacc aataccttca cccatcgaaa tcccaacagc ttgtgaagct
    53881 acgcctgttg caaaaggatt taccgttgaa gctagacagc ctacttgaga ccctactaat
    53941 acgattgcta cagctactac tgtatcaaag cctacagcca tcatgactgg gatcagtaaa
    54001 gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag agccgcctaa tgcaaataac
    54061 agcatcaata ctggaataag cattttttct tttcctttga atttcttcac aactgaagca
    54121 atcccggcat ctagtgcccc tgttttgttg ataataccta aaaatccacc aacaaccaaa
    54181 atgaagaaag agattgggat tgcagcaggt gtttctttag ttagctcccc cgctggtaca
    54241 cctagcatcc cattgattgg cgccatgaaa atatcccaca gcccttgagg attttgttgt
    54301 actcgttcat atgatccagc gacgatgttg cctgcatcgt ctacactata gtgcccagct
    54361 ggtataaacc aagtaagtac agcgattact gcaataataa taaataagac cgtatacgct
    54421 gaaggcattt gaaatttttt cttttttgtt tgttccatct tcttcttcct ttcttttccg
    54481 ttagagacaa caattttggc tctaatgtta ttttatcaaa aagagtatca gaacaaaaat
    54541 aataacacgc attttgtaaa ac
//
LOCUS       DAZUMM010000008        50029 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000008 DAZUMM010000000
VERSION     DAZUMM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.008"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..945
                     /locus_tag="ACG8E6_000427"
     CDS             100..945
                     /locus_tag="ACG8E6_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5060876.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1226..2446
                     /locus_tag="ACG8E6_000428"
     CDS             1226..2446
                     /locus_tag="ACG8E6_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS5060877.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2443..5163
                     /locus_tag="ACG8E6_000429"
     CDS             2443..5163
                     /locus_tag="ACG8E6_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5060878.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5147..6091
                     /locus_tag="ACG8E6_000430"
     CDS             5147..6091
                     /locus_tag="ACG8E6_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5060879.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6160..7170)
                     /locus_tag="ACG8E6_000431"
     CDS             complement(6160..7170)
                     /locus_tag="ACG8E6_000431"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5060880.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7350..7694)
                     /locus_tag="ACG8E6_000432"
     CDS             complement(7350..7694)
                     /locus_tag="ACG8E6_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS5060881.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7687..8121)
                     /locus_tag="ACG8E6_000433"
     CDS             complement(7687..8121)
                     /locus_tag="ACG8E6_000433"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFS5060882.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8259..8996
                     /locus_tag="ACG8E6_000434"
     CDS             8259..8996
                     /locus_tag="ACG8E6_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5060883.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8989..10200
                     /locus_tag="ACG8E6_000435"
     CDS             8989..10200
                     /locus_tag="ACG8E6_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5060884.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10404..11186
                     /locus_tag="ACG8E6_000436"
     CDS             10404..11186
                     /locus_tag="ACG8E6_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFS5060885.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11256..11918
                     /gene="trmB"
                     /locus_tag="ACG8E6_000437"
     CDS             11256..11918
                     /gene="trmB"
                     /locus_tag="ACG8E6_000437"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFS5060886.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12016..13770)
                     /gene="ade"
                     /locus_tag="ACG8E6_000438"
     CDS             complement(12016..13770)
                     /gene="ade"
                     /locus_tag="ACG8E6_000438"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFS5060887.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13902..14831
                     /gene="comGA"
                     /locus_tag="ACG8E6_000439"
     CDS             13902..14831
                     /gene="comGA"
                     /locus_tag="ACG8E6_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFS5060888.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14788..15837
                     /gene="comGB"
                     /locus_tag="ACG8E6_000440"
     CDS             14788..15837
                     /gene="comGB"
                     /locus_tag="ACG8E6_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFS5060889.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15834..16112
                     /gene="comGC"
                     /locus_tag="ACG8E6_000441"
     CDS             15834..16112
                     /gene="comGC"
                     /locus_tag="ACG8E6_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFS5060890.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16087..16557
                     /gene="comGD"
                     /locus_tag="ACG8E6_000442"
     CDS             16087..16557
                     /gene="comGD"
                     /locus_tag="ACG8E6_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFS5060891.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16529..16876
                     /locus_tag="ACG8E6_000443"
     CDS             16529..16876
                     /locus_tag="ACG8E6_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060892.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            16839..17264
                     /gene="comGF"
                     /locus_tag="ACG8E6_000444"
     CDS             16839..17264
                     /gene="comGF"
                     /locus_tag="ACG8E6_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFS5060893.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            17236..17595
                     /gene="comGG"
                     /locus_tag="ACG8E6_000445"
     CDS             17236..17595
                     /gene="comGG"
                     /locus_tag="ACG8E6_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFS5060894.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17767..18774
                     /locus_tag="ACG8E6_000446"
     CDS             17767..18774
                     /locus_tag="ACG8E6_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5060895.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18799..19983
                     /locus_tag="ACG8E6_000447"
     CDS             18799..19983
                     /locus_tag="ACG8E6_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFS5060896.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20420..22003)
                     /gene="abc-f"
                     /locus_tag="ACG8E6_000448"
     CDS             complement(20420..22003)
                     /gene="abc-f"
                     /locus_tag="ACG8E6_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFS5060897.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22108..22236)
                     /locus_tag="ACG8E6_000449"
     CDS             complement(22108..22236)
                     /locus_tag="ACG8E6_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060898.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(22581..22724)
                     /locus_tag="ACG8E6_000450"
     CDS             complement(22581..22724)
                     /locus_tag="ACG8E6_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFS5060899.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22947..23426)
                     /locus_tag="ACG8E6_000451"
     CDS             complement(22947..23426)
                     /locus_tag="ACG8E6_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5060900.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23499..23783)
                     /locus_tag="ACG8E6_000452"
     CDS             complement(23499..23783)
                     /locus_tag="ACG8E6_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060901.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23861..24055)
                     /gene="ssrS"
                     /locus_tag="ACG8E6_000453"
     ncRNA           complement(23861..24055)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8E6_000453"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24127..25413)
                     /locus_tag="ACG8E6_000454"
     CDS             complement(24127..25413)
                     /locus_tag="ACG8E6_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFS5060902.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25551..26222
                     /locus_tag="ACG8E6_000455"
     CDS             25551..26222
                     /locus_tag="ACG8E6_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFS5060903.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26188..26676
                     /locus_tag="ACG8E6_000456"
     CDS             26188..26676
                     /locus_tag="ACG8E6_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFS5060904.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26689..27636
                     /gene="prmA"
                     /locus_tag="ACG8E6_000457"
     CDS             26689..27636
                     /gene="prmA"
                     /locus_tag="ACG8E6_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFS5060905.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27637..28401
                     /locus_tag="ACG8E6_000458"
     CDS             27637..28401
                     /locus_tag="ACG8E6_000458"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFS5060906.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28634..30847
                     /locus_tag="ACG8E6_000459"
     CDS             28634..30847
                     /locus_tag="ACG8E6_000459"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFS5060907.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30858..31304
                     /gene="dtd"
                     /locus_tag="ACG8E6_000460"
     CDS             30858..31304
                     /gene="dtd"
                     /locus_tag="ACG8E6_000460"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFS5060908.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31475..32734
                     /locus_tag="ACG8E6_000461"
     CDS             31475..32734
                     /locus_tag="ACG8E6_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFS5060909.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32823..33770)
                     /locus_tag="ACG8E6_000462"
     CDS             complement(32823..33770)
                     /locus_tag="ACG8E6_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS5060910.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34164..35456
                     /gene="hisS"
                     /locus_tag="ACG8E6_000463"
     CDS             34164..35456
                     /gene="hisS"
                     /locus_tag="ACG8E6_000463"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFS5060911.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35471..37240
                     /gene="aspS"
                     /locus_tag="ACG8E6_000464"
     CDS             35471..37240
                     /gene="aspS"
                     /locus_tag="ACG8E6_000464"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFS5060912.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37349..38224
                     /locus_tag="ACG8E6_000465"
     CDS             37349..38224
                     /locus_tag="ACG8E6_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5060913.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38490..40373
                     /locus_tag="ACG8E6_000466"
     CDS             38490..40373
                     /locus_tag="ACG8E6_000466"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS5060914.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40448..40732)
                     /locus_tag="ACG8E6_000467"
     CDS             complement(40448..40732)
                     /locus_tag="ACG8E6_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS5060915.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40722..41456)
                     /locus_tag="ACG8E6_000468"
     CDS             complement(40722..41456)
                     /locus_tag="ACG8E6_000468"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFS5060916.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41585..42208
                     /locus_tag="ACG8E6_000469"
     CDS             41585..42208
                     /locus_tag="ACG8E6_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFS5060917.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42400..43521
                     /locus_tag="ACG8E6_000470"
     CDS             42400..43521
                     /locus_tag="ACG8E6_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS5060918.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43528..46656
                     /locus_tag="ACG8E6_000471"
     CDS             43528..46656
                     /locus_tag="ACG8E6_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5060919.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46675..49869
                     /locus_tag="ACG8E6_000472"
     CDS             46675..49869
                     /locus_tag="ACG8E6_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFS5060920.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLQELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
BASE COUNT        16407 a         8752 c        10438 g        14432 t
ORIGIN      
        1 gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact
       61 tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt
      121 ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca
      181 agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta
      241 gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat
      301 ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat
      361 gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga
      421 agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt
      481 gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc
      541 ccttataata atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt
      601 atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta
      661 aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct
      721 aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc
      781 gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca
      841 atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat
      901 aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt
      961 atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact
     1021 tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg
     1081 tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt
     1141 tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata
     1201 aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat
     1261 ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa
     1321 gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata acgttgattt
     1381 tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca
     1441 tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg
     1501 aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt
     1561 atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc
     1621 tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc
     1681 ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca
     1741 aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg
     1801 ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc
     1861 aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga
     1921 attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac
     1981 aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca
     2041 gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga
     2101 ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag actatttgtt
     2161 agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa
     2221 tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca
     2281 atactatcag acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga
     2341 agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa
     2401 gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc
     2461 gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag
     2521 ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata
     2581 ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca
     2641 gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc
     2701 tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat
     2761 gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact
     2821 ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg
     2881 ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat
     2941 cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta
     3001 aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt
     3061 ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa
     3121 ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta
     3181 tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt
     3241 cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa
     3301 ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt
     3361 tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa
     3421 ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag
     3481 attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa
     3541 gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt
     3601 tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac
     3661 ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca
     3721 tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca
     3781 atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac
     3841 aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa
     3901 gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa
     3961 aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat
     4021 cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata
     4081 gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa
     4141 gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa
     4201 gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag
     4261 atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg
     4321 aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta
     4381 tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg
     4441 aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa
     4501 gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa
     4561 gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga
     4621 gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa
     4681 ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca
     4741 gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt
     4801 ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac
     4861 ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg
     4921 gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt
     4981 gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct
     5041 aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat
     5101 taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg
     5161 tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag
     5221 aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat
     5281 cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt
     5341 cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca
     5401 tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc
     5461 tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc
     5521 gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt
     5581 tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac
     5641 aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt
     5701 gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc
     5761 gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat
     5821 aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg
     5881 tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat
     5941 aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt
     6001 gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa
     6061 ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa
     6121 aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt
     6181 ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga
     6241 agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac
     6301 attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac
     6361 tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc
     6421 tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta
     6481 ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt
     6541 caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt
     6601 aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga
     6661 gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa
     6721 tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc
     6781 atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata
     6841 ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc
     6901 taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc
     6961 atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga
     7021 ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc
     7081 gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt
     7141 agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta
     7201 tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc
     7261 aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat
     7321 agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca
     7381 gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta
     7441 aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt
     7501 tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc
     7561 agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg
     7621 ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa
     7681 aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc
     7741 ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct
     7801 gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt
     7861 gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat
     7921 cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc
     7981 tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa
     8041 agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt
     8101 gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat acttcagctt
     8161 aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg
     8221 tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg
     8281 tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg
     8341 gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca
     8401 ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga
     8461 aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg
     8521 aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg
     8581 atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg
     8641 actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct
     8701 ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag
     8761 ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga
     8821 tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata
     8881 acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg
     8941 attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt
     9001 tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg
     9061 aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct
     9121 aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt
     9181 tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt
     9241 tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct
     9301 tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg
     9361 gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg
     9421 aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt
     9481 cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat
     9541 tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt
     9601 ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat
     9661 aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga aattacggca
     9721 aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa
     9781 aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt
     9841 tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt
     9901 agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg
     9961 aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag
    10021 caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg
    10081 attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt
    10141 gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga
    10201 aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc
    10261 tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga
    10321 acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa
    10381 agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac
    10441 gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct
    10501 ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa
    10561 aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg
    10621 aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt
    10681 accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca
    10741 tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa
    10801 atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg
    10861 aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt
    10921 atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa
    10981 cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca
    11041 tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc
    11101 aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac
    11161 aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga
    11221 cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag
    11281 cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg
    11341 gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga
    11401 aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat attggagtag
    11461 aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta
    11521 acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag
    11581 tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc
    11641 gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa
    11701 tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat
    11761 atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata
    11821 ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag
    11881 ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa
    11941 aaacattaat atccataaat aaatcccaga aaatgaacat tacagatgta atgtattttc
    12001 tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa
    12061 ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga
    12121 taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg
    12181 tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat
    12241 gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat
    12301 cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc
    12361 aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag
    12421 ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac
    12481 cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac
    12541 tttttctacc aaatctttag tcactagact attcgggata atttcgatca catgggcttg
    12601 tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg
    12661 cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga
    12721 ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc
    12781 tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa
    12841 acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc
    12901 aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt
    12961 atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga
    13021 tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg
    13081 ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata
    13141 aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg
    13201 gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt
    13261 catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc
    13321 atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa
    13381 aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc
    13441 agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga
    13501 tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat
    13561 gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc
    13621 gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa
    13681 gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac
    13741 ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa
    13801 gtataacata aattcggttt ttgccaatat ttttttgtaa attcatttta tttgaaacaa
    13861 aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat
    13921 cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag
    13981 cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag
    14041 tctcttcggc agatggacag caactgatct caagatttaa gtatttggga cagatggata
    14101 tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag
    14161 ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc
    14221 tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta
    14281 ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca
    14341 agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag
    14401 atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac
    14461 agacttatga tacacttttg agactcgcat tacggcatcg tccagacttg ctaatcattg
    14521 gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc
    14581 gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac
    14641 tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt
    14701 tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat
    14761 cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag
    14821 aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa
    14881 ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg
    14941 tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt
    15001 gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa
    15061 cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa
    15121 ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg
    15181 ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg
    15241 gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta
    15301 ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct
    15361 cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat atattccttg
    15421 tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta
    15481 aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc
    15541 tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat
    15601 cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta
    15661 ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat
    15721 cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta
    15781 gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa
    15841 agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga
    15901 tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag
    15961 accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc
    16021 ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac
    16081 gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg
    16141 ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt tccagttctc
    16201 gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa
    16261 cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg
    16321 aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa
    16381 gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct
    16441 agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg
    16501 acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac
    16561 aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt
    16621 agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct
    16681 atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga
    16741 gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga
    16801 aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag
    16861 catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat
    16921 tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa
    16981 aagagtggct aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct
    17041 ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat
    17101 atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta
    17161 tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa
    17221 atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc
    17281 tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca
    17341 ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca
    17401 aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga
    17461 gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta
    17521 gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat
    17581 actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt
    17641 ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg
    17701 tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg
    17761 tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag
    17821 cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat
    17881 ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa
    17941 cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc
    18001 agactgtctc aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat
    18061 caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca
    18121 aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta
    18181 acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt
    18241 gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa
    18301 tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc
    18361 gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc
    18421 gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag
    18481 ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa
    18541 gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat
    18601 gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca
    18661 caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag
    18721 aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc
    18781 aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg
    18841 aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt
    18901 atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa
    18961 atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa
    19021 ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga
    19081 ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa
    19141 ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa
    19201 aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg
    19261 ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga
    19321 aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg
    19381 aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc
    19441 acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc attagctggg
    19501 gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa
    19561 aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta
    19621 ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat
    19681 attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg
    19741 acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga
    19801 catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg
    19861 ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta
    19921 ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc
    19981 taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg
    20041 tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt
    20101 acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt
    20161 ttatttattg attttcagga aaatcccaat attttatttt cattcatctt tataagggac
    20221 attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt
    20281 cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa
    20341 cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt
    20401 tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct
    20461 cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga
    20521 ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc
    20581 tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt
    20641 tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat
    20701 cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca
    20761 atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca
    20821 aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac
    20881 cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat
    20941 gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct
    21001 ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt
    21061 gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt
    21121 ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa
    21181 aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg
    21241 tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact
    21301 caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc
    21361 gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc
    21421 gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg
    21481 ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc
    21541 gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta
    21601 atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact
    21661 tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga
    21721 ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg
    21781 taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt
    21841 cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac
    21901 cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat
    21961 tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga
    22021 ccactcaaaa aggccatggt tatccatggc ctgtgttatt taaactcaga ttatcatgag
    22081 aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt
    22141 cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta
    22201 attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt
    22261 tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa
    22321 tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc
    22381 gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt
    22441 gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa
    22501 cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa
    22561 tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc
    22621 tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc
    22681 gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa
    22741 gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca
    22801 ctttttttta ttttacttga actcattgac tgttttccct ttattttaaa cattataaag
    22861 accgatgagt gcttgccgcg tcttttgttt ttatcacggt tttttgtgca aaaaaacctg
    22921 ccgatagttg gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc tggcaattgg
    22981 ttttctaaat cagttcggac aacaagcaca tcgcaagctg cgtttctaat aacatattca
    23041 gaaacagaac caataaataa acgttctaca gcattcaatc ctgtggctcc taacataatc
    23101 aaatctactt gattatcttc tgggatttct ctggcaataa tttgttttgg tgagccatat
    23161 tctacgacag aggttacatt attcaagcca gctttcttcg cattgctttc ataatctgcc
    23221 aatgtttgct ttgccatttc agtagcttgt tctgcaagca ttccatcaaa ggatgaaacg
    23281 gtttggaaag aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt atttcggata
    23341 gctacattga ccgccttttt aaaagctaat tcagcttcat cagaaccatc tacggcaacc
    23401 atgatttttt tgtattgttg taacataagc attcactcct caagtcaatc gtattgtact
    23461 cttttattgt agaacacttt agggaaaaag agaattattt actgctttgg ttcagcaatt
    23521 ttttttgcaa atcggatact gaaggtggca gaaacaataa ttacgatcag taaaaagacc
    23581 aaggcaataa agcgttgatt gaaaaaagct gttaaaatac ccattacgcc taaaacagca
    23641 tatgcagctc cgtaaaattg taggaatcct tgatttttat catttttgtt gatcaataca
    23701 aaaaagatat cctgtttatt aaataaataa tagctaacga ataataatac taaagtggcg
    23761 ataatcatta aaatcctaat catgaacaaa tcctccaaaa taagcaacaa atagaaaaag
    23821 caatttgcca agagcaaatt gctttattct ttaaaagatg gtaatctccg ttaatgccgt
    23881 tgtttgtcct gtagtattga tcccgcgaat tcaagcaggt gggtcccatg gtcgtagctt
    23941 cgaactacca aatgataagg catgttacca gcagtgacac aagacgggtc ccaagatatc
    24001 aataaaaatg ttcggtcaac acacaaagag gacaattcga acatcacaga tttcccacct
    24061 ctatagtagc atatgaattt aaaaagagca atgagagagc tcgcagaaac agaaccgttt
    24121 tcttgtctag ttttttggag gatgcttttt ccactctttt atacggtaat actgttgacc
    24181 cagagcctgt tcatatttcc ctgtatcttt tggctgataa tactgagcat tcttcagttt
    24241 atctggtaaa tattgctgat tgacccaagc ctgatcaaaa tgatgaggat actgataacc
    24301 cacgccacgg ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat gatcaggaac
    24361 atctccagct ttgccctctc gaatatcagc aagtgctgca tctaatgcca tgtaggcaga
    24421 attggatttt ggcgacaagc aaagatcgac gacgacgtct gctagtggga tacgtgcctc
    24481 aggcaatccc aatttttcag cagctaatac agcgttcact gtacgagccg cagcagcagg
    24541 attacctaaa cctatatctt cataaccgat aaccatcaga cgccggcaaa tacttgccag
    24601 atcacctgcc tcaactaaac gggcgagata atgaagtgca gcatccacat cacttccacg
    24661 aatcgatttt tgaaaagccg aaataacgtc ataatgagca tctccatttt tgtcatgggt
    24721 aagagctttt ctttggacac attcttcaat gatcgacagt gtaagatgaa tccttccttc
    24781 tttatctgaa ggagtcgata gtgtagccag ttccagccca ttcaatgcac tccgcaaatc
    24841 gccgttcgtt gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga ttgcttgttg
    24901 tccaaggccg cgttctttat cccttaaagc atgttcgact gccaactgga tgtcttgttc
    24961 tgttaatggt ttgacttcaa agatctgtgt acggctgcgg atcgctggat tgatggtaat
    25021 ataaggattc tcagtagtcg ctccgatcaa gatgattcgg ccactttcta aatgaggaag
    25081 cagaaaatcc tgttttgttt tatctaatcg gtgtacttca tccaataaga ggatcactgt
    25141 accactcatt tttgcttctt ccgctacaac ttgtagatcc ttttttgaat ctgtagcggc
    25201 gtttagcatg cgaaatgcat aattagtaga gccggcgatc gcacttgcga tactcgtttt
    25261 tccagtacca ggaggtccat atagaatcat ggaagagagc atgcgagcat cgaccatgcg
    25321 gcgtatgatt tttccttccc cgactagatg ctgctgacca acgacttcat cgatcgttcg
    25381 aggtctcatg cgataagcca atggctgttg cataggattt tcctccttct tgtttttacg
    25441 tcccttaatt atatcatatt tagcaagatg cctagaaaga acagccttct gttgttttgt
    25501 tttcatagct tatccaacta atattgtgta tgataaagta ggtgagaaaa atgacggata
    25561 aacaaaagat agatgaattt ttttggaata aaacgacgcc agaagtggca cgggatttat
    25621 tggggatgta tttggagcat gacacaccag aaggaagatt agctggatat attgtagacg
    25681 cggaagcata cctaggtcca gaagacgagg ccgcccatag ctatggttta agaagaacac
    25741 cgcgtgtgcg ggcaatgtat gaaaaaccag gtacgatcta cctttacacg atgcatactc
    25801 atcggatttt gaacatcatt actcagccag aagggattcc tcaaggagta atgatacgtg
    25861 caatcgaacc cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga aaaactggtc
    25921 cagatatcag caacggtccg ggaaaattag tggaagcatt agcaattcct caagaacttt
    25981 atggtcagtc tatagcagat agttcgttac gcttagtttt tgagaaaaaa aagacaccaa
    26041 aaaagatcaa tgctttgcct agaatcggta tcccaaacaa aggtgtatgg acagaaaagc
    26101 cgttgcgttt tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa aaccaaatcg
    26161 agaaagattg gggttggagg aaagaaaatg aaaaagaagg atctattaac atatttagat
    26221 gaaataattg aaaaacaagt gacagactat gatgtagctt tagattggga tacaaaaaat
    26281 catacgatcg agatcgttgt tcgcttgttt gcagaaaata aagcacaatt tgagattgat
    26341 gatgcagaag ggatcgtttc gcaagaacca attatcgaat ttgaagacgg cgtacttctt
    26401 tttaatcctc agaaatctgt ttttgatgaa cgcaattatc tagcagtaat cccttatgaa
    26461 ggaaaaaaag ggttggcaaa atcagtagct gattctttag tggaatatct aaatgaagta
    26521 ttagctcaag ggcaaagcga tttgcttgat tttttggatg aagacgatga cgaagctgta
    26581 ttcgagcttc actgggataa tcagcaacta gcagaattag tagaagtaaa acagcaaaat
    26641 gaagcagata cttatttacc gtacccaagt tattaaggag tgacagatat gaaatggaat
    26701 gaagtaaaag tagaaactga aagtgaagca gtcgaagcag ttgctaatat tttgatggag
    26761 gcaggggcga gtggtgtcgc gattgaagat tctttagatg ttgaacattt tcagtcggat
    26821 caatatggtg agattttaga taaaaatgat tttacatcgc tgaaagaagg cgcactagtc
    26881 atggcttatt ttccggaaac gactttctta ccggaaatct tgccttttat caaagatcgt
    26941 atcacacaat tacctgagtt tggtctagct attggaaaaa ataaagtaac agttagtgaa
    27001 gtagaggaaa gcaactgggc aacagcctgg aagaaatact atcatccggt gcgtattacg
    27061 cgaatgctga cggttgcgcc aagctgggaa aactaccaga cagaagatcc tctagaaaaa
    27121 attatcacac tagatcctgg tatggcattt ggcacaggca ctcatcccac gacttccttg
    27181 acattgacag cactagaaac agtcttgcgt ggtggtgaaa cattgctaga tgtgggaact
    27241 gggtcgggcg tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt ctatgccttt
    27301 gatttagacg aagtagccgt tcgttcggcg aaagaaaata tggatatgaa tgaaatagcc
    27361 aaagatgtcc atgtatcagc taacgatctt ttgacaggaa tcgagattga aagtgatgta
    27421 atcgttgcga acattttagc agatatcatc ttattgatga tcccagatgc ttggcgttta
    27481 ctgaagcaaa caggcacgct gatcgtttca ggaatcattg aagagaagaa gcaaatggtt
    27541 ctggatgcca tgttagaaca agggtttatc gtggatcaaa ttttccagca aaaagactgg
    27601 tatgcaatta ttttgaaaaa accagaggtg gaatagatgc agcggtattt tttaaaagaa
    27661 tcatatgaat caaaagacta ttttgtttta gaaggggaaa actatcacca cattgtacgg
    27721 gttatgagga tggaacccga ggatgaagtt tttttggcat tcaatgatca aatagccatt
    27781 gttgcagaaa tcacggagat tactgaagag gctgtttatc tgaaagaaat taaaaaagaa
    27841 gaaaatcaaa aggaactgcc aagttcgatt accatagcga gtggctatcc taaaggggaa
    27901 aagctggatt ggatcgttca gaaatccact gaattaggag cgcaagcttt tcttggtttc
    27961 ccggcatcag cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg acaacaacgg
    28021 cttgaaaaga ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt gccatcagta
    28081 gagttattgg aaacgaaaca agatttattt gagcggctag atgattatga cgtggtcttg
    28141 gttgcttatg aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa aattcttagt
    28201 gaaacaaaac cagatagtcg aatcttagct gtctttggac cagaaggtgg attatcccgc
    28261 gatgaagtcg attattttgt agaacacaaa gggatacttt gtggattagg accaagaatc
    28321 cttcgtacag aaacagcacc tctttattta ttaagtgctg ctagctatca atgggaatta
    28381 atgaatcctt ctttcagcta acgttcttca ttaataaaag agtatcgaca gaaagaattg
    28441 aaaaaatagt caacaatttg tataatagaa acaattattt atacaatgga tacgaaaaat
    28501 cgtttgaaaa agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa ctgcacggat
    28561 tttgggacaa ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa aaaagaaagg
    28621 gggatcgatt cctgtgacaa aagaagaaat tttaacggga cccggtgtca taaagctcgt
    28681 tagtcaatac atgggtcccg aacatgtagc atttgttgag aaagcatgcg aatacgcgac
    28741 tgctgcacat gacggacagt ttagaaaatc aggcgaaccc tacattattc atcctatcca
    28801 agtcgcaggt atattagcag atttaaaaat ggatccccat acagtggcta caggcttctt
    28861 acatgatgtt gttgaagata cagaaatcac tttagaagat ctgagagaag aatttggcga
    28921 tgacgttgct atgttagtgg acggcgtaac caaattaggg aaaataaaat ataaatccca
    28981 cgaagagcag ctagcagaaa accaccgaaa gatgctgcta gcaatggctc aagatttacg
    29041 agttatcatg gtcaaattag ctgatagact acataacatg cgtacgttga agcacctgcg
    29101 agaagataag caaagaagga tcgctcagga aactttagaa atctatgcgc ctcttgcaca
    29161 tcgtctaggg atcagccgga tcaaatggga attagaagat acagcacttc gttatctaaa
    29221 tccaaaacag tattaccgta tcgtccactt gatgcagacg aagagagaag aacgtgaaaa
    29281 atacgtaagt ggtactgttg aagatattcg aatagcgaca gaagagctgg ggatttttgc
    29341 agaaatctat ggacggccaa aacacattta ttcaatctat cgtaaaatga aagatcagaa
    29401 aaagcagttc aacgaaattt atgacttgct agctattcga gtgatcgtag attcaatcaa
    29461 ggattgttat gctgtattag gagcaatcca cacaaaatgg aaaccaatgc ccggcagatt
    29521 taaggattat attgccatgc caaaagctaa tatgtaccaa tctttgcata ctaccgtgat
    29581 cggtccggca ggaaatccgg tagaaattca aatccgaaca caggaaatgc atgaaatcgc
    29641 tgaattcggg gttgctgcac actgggccta taaagaagga aaaaatgaaa aagtagaacc
    29701 agatggtatg acgaaacaat taagctggtt ccatgagata ctcgaacttc aagacgaaag
    29761 ctatgatgct tctgaattta tggaaggcgt aaaaggagat atctttagtg ataaagtcta
    29821 cgtcttcaca ccaaaaggag acgttactga gttaccaaaa ggatccggac cattggactt
    29881 tgcatacagt atccatacag atatcggtaa caaaaccact ggtgcaaaag taaatggcaa
    29941 aatggtgcag cttgattaca aattgaaaaa cggagatatc attgagatca tgacttctcc
    30001 aaattcattt ggcccaagtc gcgactggtt gaaattagtt gctactagca aagcaagaaa
    30061 taagatcaaa cgtttcttca aagcccaaga tcgagaagaa aatgtgatca aaggccacga
    30121 atccgtggtc aaatgtatta cagatctagg atttacgcct aaagatattt tgacgaagaa
    30181 caaactgcaa gaagcactcg atcgttttaa ttatcaaaca gaagatgatc tctatgcagc
    30241 tgtagggtat ggagaagtta gccccttgac gatggccaat cgtctgactg aaaaagaacg
    30301 taaagaacaa aaaatcgagc agcaaaagca agaagcagaa gaaatcatga atcagccgaa
    30361 aaaagaacct gacaaaatga aagtacgtca tgaaggtggc gttgtcattc aaggggtaga
    30421 aaacttacta attcgtatca gtcgctgctg taatccgatt cctggtgatg atatcgttgg
    30481 ttatatcact aaaggcagag ggatatccat tcatcgtcga gattgtccga atgttcagcc
    30541 tgacaaacca aatgtagcag aacgtttgat tgaagtcgaa tgggaagata catcgaatac
    30601 acgaaaagag tatgatgcag atttggaaat ttacggctat aatcgttcag gcttattgaa
    30661 tgatgtactt caaacagtca atgcgctaac gaaaaatctc aacagcgttg aagcacggac
    30721 gaataaagat aaaatggcga cgatccattt gacggttggt atccagaatt tatcccacct
    30781 aaagagtatc gtggataaaa tcaaagcagt acctgatgtc tacagtgtac gccggacgaa
    30841 tggataggag gacagatatg agagcagttg ttcaacgggt aagtaaagct gctgtgtcag
    30901 tcgatcagca agaaattgga aaaattggct acggcctact tgttttatta ggcattcatg
    30961 aaaccgatac gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa atgcgtattt
    31021 ttgaagatga acaaggaaaa atgaatatta gtgtagaaga tgtgggagga caaattttaa
    31081 gtatctctca attcacactg tttgcggata cgaaaaaagg aaatcgacca agctttgtgg
    31141 aagccgcacg accagaaaca gctattcctt tatacgaatc attcaatgaa ggaatcagaa
    31201 gtcgcggcat cacagtcgct accggacaat tcggagcaga tatgtcggtt tctctcgtca
    31261 atgatggacc tgtaacgatc attattgatt ctaaaaacaa ataaagtaat ctggttttca
    31321 ctagtaaaaa ctatgaaaca cgtcgcttaa gttagactga taaactctaa cttaagtggc
    31381 ttttttgtct gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt aagcttaaat
    31441 tatacaagtt attttttata ggaggttgct gaagatgaaa gaagtaccag tagtagatat
    31501 caaaatcaca gatttaaaaa aattgtatca aaaaggatac aacatgttgg aggagttacg
    31561 ccatgaagcg gacgctcccg tcgtcaaagc aaagattttc aataaagaag ctatcacgat
    31621 ctacggatca tcagcagcaa aagtatttta tgatccaagg aatttcaaac gaaaaggtgc
    31681 tatgccaaaa ttagtattga agacactttt tggtcaaggt ggagtacaga ctttggatgg
    31741 agctgctcat caccatcgga aaaatatttt tatggatcta atgacaccgg aaagaatgga
    31801 agattatcat cgtatcttag ataagaatct gacacaagca cttgaagcac agcatggtca
    31861 atttgaacta tttgatttaa gtaagatggt tttctttaca tctatttgcg agtgggcagg
    31921 tatcaattta tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat atcaaatctc
    31981 tatgatcagc ggaacgttta cttctccgat tgatcacata aaaggtgtcg aaaatcgcaa
    32041 aaaatcagaa aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc ctgtggctgg
    32101 aaaagaaaat gtggcattat atgcttttgc caatgccaca gatttagatg gtcagctttt
    32161 acctctggaa gtggcagctg ttgaattatt gaacatcatc cgtccaacgg ttgctctaac
    32221 tgtgtgggcg gcattgatgg gacatgcact tttcagtaga ccagatttgt atcagcaatt
    32281 aaaaaatgac ttttcgacgt tacaagatcc gtttattcaa gaaatgagac gctattatcc
    32341 tttcttccca atgctacctg cgatttcgct gaaagaagta gaagtagatg gttatcgtat
    32401 accagaaggc agttgggtta ttttagattt atatggaaca gaccatgatg aacgaacagt
    32461 tgaagcgcca gattccttta tgatcaaacg gtatgtaggg aaagcaaaag atatttccta
    32521 taaagaagaa tatgagatga ttgcacaagg cgggggaaat tttcgacaaa tgcatcgctg
    32581 tgcaggagaa tggatcactc ttcatagtct acgtgtgttt tccgatcagc tagtaaataa
    32641 gtttgagttc tctgtacctg aacaagattg gacgattcca ttcaatcagt ttccgactta
    32701 tccgaatagc cgagcgctac tttacaagaa ttagattagc ataaacttat cgaaaaaaag
    32761 gaagatcaaa cagtgctgat cttccttttt tcatattact ataaattgat aaggtcaatt
    32821 gatcaaaatc cacgaaatgc ttcatcaatg gtttgataat cttcattgga taaatgcaaa
    32881 tctaaagctt ggacattgtc agatacttgc tctggacgct tagctccagg tataactaca
    32941 gacactcgtg gattcttcat gtaccaagct aaaacaagtt gcgcaggagt ggcttggtat
    33001 cttttagcaa tggggcgaag tacatcgaca gcggttaaaa tttctctgaa acgttctcct
    33061 tggaaatctg gatttctttt tcgaggatct ccttcaccaa attgtttttc ctcaccaagt
    33121 tcatattttc ctgtcagcaa accggaagca agaggaaaat agggaacaaa actgattttg
    33181 tttttttcaa gataagggaa taattctttt tcagcctgcc gatggatcaa actgtattta
    33241 tcttctacta catcgacata tccatctgca ttcgcttctt tcaattgttc taacgtgaag
    33301 tttgacacac cgactgctcg gatttttcct gcttctttta attcgtgcaa tgtagcaact
    33361 gattcatttt ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc gatgtaatca
    33421 gtttgcagac gttttagtgc atcttccacc gattgtttga gaaactcagg agaattatca
    33481 aatgttcgcc ctttatttgg tacatgagca gcttttgtag cgatgacgac acgtgagcga
    33541 tcatattctt tttccttcag tacctcgcca atcagttctt cggaacggcc gtttccgtaa
    33601 gcataagcgg tatcgatcaa attgatcccg ctattcaaag ctgtacgaac tacttgtttt
    33661 cctgtctcat cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt tgctcctaat
    33721 ccaagctgtt ctgcgtagac ttgtgaatgt ccgattctta tttctgccat agctgattcc
    33781 tcctttttgt ctagtctaaa acaaaatacc aaaaagctga actaaaacgt ttttgtgagg
    33841 ggaaatagtc agatatctct tgactttgag actggttttt agtaggttat aagtaatatc
    33901 gaatgtcaat gaaagagcga gtaagttttt gaaactttac aagagaggat catcgaggct
    33961 gaaaatgatc taaagtttca ttagcctaaa gacactctgg agatggacga gtgaaaagcc
    34021 gtcccgcgta cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat taggtggtac
    34081 cgtgtatgaa tttcattcac ccttgttcat gatgaacaag ggtgattttt ttatttacat
    34141 agctaagaaa gggagagaaa actatgagtt tccaaagacc aaaaggaaca aatgatattt
    34201 tgccaggcga atcagaaaaa tggcagtttg tcgaagaaac agcacgttta ttattcagag
    34261 attatcagta taatgaaatg cgtacaccga tttttgaaca ctatgaagtg atatcccgaa
    34321 gtgtagggga tacaacggat atcgtttcaa aagagatgta tgatttttat gacaaagggg
    34381 aacgccatgt gacgcttcga ccagaaggta cagcaccaat cgttcgttct tttgttgagc
    34441 ataaattgtt cggcccagag tatgcgaaac cgttcaaaac tttttatatg ggaccaatgt
    34501 ttcgctacga acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc ggagtagaag
    34561 catttggatc agaaaaccct gcaactgatg tggagagcat ggtgatggca cttgatttct
    34621 tcaaacagtt ggggatcacg caaatccggc tagtcatcaa ttcattaggt gataaagaaa
    34681 cgcgtcaagc ctatcgtcaa gcattgatcg actatctttt accttttgaa tcagagctga
    34741 gtgaagactc taaacgccgc cttcatgaaa atccattacg cgtattggac agtaaagaca
    34801 aacgagatca aaaatttgtg gcagaagctc catctatctt agattatcta agcgagcctg
    34861 ctcaaaaaca ttttgatacc gttgtttcca tgctggatgc attggctgtt ccttatgaaa
    34921 tcgacagtaa tatggttcgc ggactagatt attacacaca tacgattttt gaaatcatga
    34981 gtgacgcacc aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt tatgacaatc
    35041 tagtggaaga actaggtggt ccttcaacac ctggcttcgg atttgcaatg ggtatcgagc
    35101 gattactctt gactatggag gcagaggaag tagttatccc tgcatttaat gagttggatg
    35161 cttacgtggt cgcactcgga gacgaaacaa atatcgaagc attgaaagtg gtccaagcga
    35221 ttcgaaactt tggtttttca gctgaccgcg acttcatgaa ccgtaaagca aaagcgcaat
    35281 ttaagacagc agataaagcg aatgccaaat tagtattggt gatcggtgaa gatgagcttg
    35341 ctagtcaaac gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt tttccattag
    35401 aagagatata tgaacatttt gatgaagtct atgatgaaat gactttattc gagtaggagg
    35461 aaaaaaataa atggcacaaa gaacgatcta ttgcggatta gtttccgaac aacaattaga
    35521 acaaacagtt acattaaaag gatgggtaca aaaaagacga gaccttgggg gagtaatctt
    35581 tatcgaccta cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg aaatcaacaa
    35641 agaagcatgg gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag tgactgggga
    35701 agttagaaga cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg aattcgaagt
    35761 gattgcaagt gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc aaattgaaga
    35821 tgatcaagca atcagtgacg aaatacgttt gaaatatcgt tatttggatc tgcgtcgtcc
    35881 aaaaatgact aaaaacatca aattaagaaa tgcgacaaca aaagcgattc gtcattattt
    35941 agatgataat gactttctcg atatcgaaac accttaccta gggaaatcta caccagaagg
    36001 cgcaagagac tacctagttc cttcacgtgt ccatgctgga catttctatg cgttgccgca
    36061 atctcctcaa ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt attatcaaat
    36121 cgttcgttgt ttccgcgatg aagatttacg tggagaccgt cagcctgaat ttacgcaagt
    36181 agatattgaa acaacgttct taacagcaga agaaatccaa acttacacag agggattgat
    36241 tgcgaaggta atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc ctcgtatgac
    36301 ctatgatgaa gcaatggctc gctacggcag cgacaaaccg gatacacgtt ttgatatgga
    36361 attgatcgat ctaagcgata cagtcaaaga agtagaattc aaagtcttcc aaatggctct
    36421 tgaaaacggc ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc gctactcacg
    36481 aaaagacatg gatcagttag gtcaatatgt aggacaattc ggagctaagg ggcttgcatg
    36541 gctgaaagta gaagaagacg gattgaaagg tccaattgct aaattcatgg gagaagcaac
    36601 agaagcaatc attaaagcaa cagatgcaaa accaggtgac ttgctgatgt ttggcgcaga
    36661 taaaagtgaa atcgtcgctg ctgcattagg tgctatccgg acacgtttag gaaaagaatt
    36721 aggactaatt gatgaatcga aattcaattt cttatgggta actgattggc cacaatttga
    36781 attttcagaa gaagaaggtc gatatgtctc tgctcaccat ccattcacga tgccaaaaga
    36841 agaagatatc ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag cttatgatat
    36901 cgtcttgaac ggttatgaac taggcggagg ttctatccgt attcatactc gtgagcttca
    36961 agaaaaagta ctagaaactc taggctttac aaaagaacaa atggaagaac aatttggttt
    37021 cttgttaaca gctttagatt acggattccc tccacatggc ggtattgcat taggattgga
    37081 ccgttttgca atgttattag ccggagaaga taatattcgt gaagtcattg cctttcctaa
    37141 aaacggaaaa gcagcagatg tgatgagtga tgcaccaagt atggtttcgc ctttacaatt
    37201 gttcgaattg aatattgatg taacgaatat agaagaatag ccgataaaaa ggtaaaaagg
    37261 atttgtcgaa tacgcttctt ttgacaacct tgatttaatt gaaaaacact ttataatgaa
    37321 gccataacat ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt gggcttatca
    37381 tgattacacg accaagcttt cggtatctat cgtttatgcg ttgatcgcat cagtcgcatt
    37441 gaatttcttt tatcagccag gacatattta ttcaagtggt attactggtc tagctcagat
    37501 tttgacgact ttgtcaaaat cactgatagg attcgaagta ccggtatcaa caactttgta
    37561 tttactgaat atacctttgt ttgtattggc gtggttaaaa atcgggaaga agtttacaat
    37621 tttcactttt atcacagtca ctttgacctc cctttgtatc catattttac cggaaacagt
    37681 cctatctacc gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg gactaggtat
    37741 tgggctagcg ttgagaaacg gcctatcatc gggcggtcta gatatcgtga gtatcacgat
    37801 tcgtaaaaaa acaggaaagt cgattggttc tatttcgatc tacttcaatg cattgatcgt
    37861 ttttgttgcc ggaattctat ttggctggca atatatgttt tacagtacgt tgtctatttt
    37921 cgtcagtgga aaagtaactg atgtcgttta tactaaacaa aagaagatgc aggtaatgat
    37981 cgtaacgaaa catccagaaa aagtagtaaa tgaaatccaa aactgtatgc gtagagggat
    38041 cacgatcatc aatgaagcag aaggtgctta taagcatgat aagcagacag tattgatgac
    38101 gatcgttaca cgatacgaac tgcctctact tagagaagca atgaaagctt ctgatccaca
    38161 agcttttgta agtattgcag aaaacgtcca gatacttggt cgtttctatg aggatggcat
    38221 ttagatcgag tttgacaaaa attcatgaaa tactgctgta gacagacgtt cgtttgtctg
    38281 cagttttttt ctaccatagt agaatcagca aagaaaaact gcttttttat ttgaaatttt
    38341 cttaaagttg aaaaaaagca tgtttatttt tttaaattta tgggatgata gaaaaaaata
    38401 gtagagaaag gacacgattc tacttgctat tttcacttaa tacccgtata ttacgaatga
    38461 aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc
    38521 gctacatcac tgggtttgat gggctccgtt cattagctgt ggtgggtgtt atcttctatc
    38581 accttctgcc aactagtatg aaaggcggct atttgggcgt tccgattttt ttcgtcgtat
    38641 ctggttattt gatcacggat ctgctgcggc aggaatggga acaaaatgga aaaatcaata
    38701 tttggcaatt ttatgtcaga agaatgaaga gactctatcc ggcacttgtt tttatgcttg
    38761 tgacagcttc ggcttatatc actttgtttc agcgagggct gttaaacaat cttcgaggag
    38821 ttgtggtcag cagcttactg tatgtcaaca actggtggca aatcaataac ggtttatctt
    38881 atttcgatcg atttgctaat gagtcaccgt ttacgcatat ctggtcttta gcagtagaag
    38941 gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa
    39001 gaaaatggat cttttatact gtgttaggta catccttcct ctccgccatc ttgatgatga
    39061 tcttgtatac accaggaggc gatccaaccc gtgtttatta cggtaccgac actcgtctgt
    39121 tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa
    39181 acatcccgaa acaagcgaaa aatattctga atatcgctgg aggaatctcg cttttggcac
    39241 tgattctttg cttcttcttt ttagatgatc attacaattt catctattac ggaggaatgc
    39301 tcctagtgag tctgttatgc gtagtgctag ttgcagtgac agcacacccc ggagcaagtt
    39361 tgaatcgatg gctgacaaat ccagtattta cttggattgg caaacgaagc tatggcattt
    39421 atctgtatca gtttccagtt atgatttttt atgaagcaaa ggtgactgat attgccgatc
    39481 atgttttgtt gcatacttta gtggaaatca cattgattct aatcatcagt gaactttctt
    39541 atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc caagtgatga
    39601 aaggatggtt cagcaaaccg atcatcagta agagcaaacc atgggttgtt ccaggcacat
    39661 tacttgtagc aattgcactc gttggtttcg ttacggcacc gaaaaacgct gtaacagccg
    39721 atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa
    39781 acaattcaga aaacaccact agttcggttg accaagcgat ccttgataag tacgagttgt
    39841 ccaaggcaca agggaataag gcacaaaaat tagaactaac ggcatttggt gattcagtta
    39901 tgttggatgc tgcaacggat ctacaagaac tttatccaaa agtagtggta gatggagatg
    39961 tcggacgaca attatatgcc agtgtaccgt acattgaaaa attaaaggaa caagatttgt
    40021 tgaaagacac tgttctgatc ggactaggaa cgaatggctc attctcggaa gctcagtttg
    40081 atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga
    40141 cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca
    40201 atatgacgct gattgattgg tacagctaca gtaatgacca tagtgactgg ttttatgatg
    40261 atcaagtcca tccaaatcct gaagggatga cacattacat tcacttagtc agcgaagcct
    40321 tgctaggagg aagttcaagt acgaacagca gtgagacaaa cgtgacagaa tgatagctac
    40381 aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt tttttatatt taccgtgttt
    40441 ttaacattca tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt
    40501 tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag
    40561 aaaatacttc tgcatggatc atcttaacag gtcttctttt ttgaagacga gtatatttag
    40621 caccagtacc attgttatgt tcctctaatc gtctagaaag atcggttgta tatccaccat
    40681 aaaatgtctg atctttacag aataatacgt aaaaataatg ttcatttgcc atagagcatc
    40741 tcacgaatct ctgcagtgta ttctccatcc atattgtaag tgatcaatgg tggagcgact
    40801 ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta acaaaatatt tgcctctttt
    40861 ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc
    40921 tgaatgattt ctaagaaccg atcaggacga tggaccatag taaagcgtcc atttgttttt
    40981 agtagcttac ttgaaatatc gatcacttct tctaacgtcg tatgaatctc atgacgggcg
    41041 attgccaggt gtttattagg gttcgtttta tttgttggca gccctttgaa ataaggtgga
    41101 ttacagacga ctaagtcaca agaattatgt cgaacgacag ataagctgtc tttcagatcg
    41161 atggtgtgga cagtcatttg ttcatttaaa tggttaagcg cgatactacg tcttgccatg
    41221 tctgctagac gaggttgaag ttcgatagcg tcgatagccg cctgggttcg tttacttaaa
    41281 aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca
    41341 cgggaaaagt gagcaagcag cacagcatct aaagaaaaag aaaacacttt ggaactttgg
    41401 ataatcttga tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg aagcatggat
    41461 tccctccaaa atgaaaaaaa tagtcgtctg tattatacta tttttattaa aaagaaccaa
    41521 ggtggcttgt caaacctcta ttttttatgc atactatgac cataagaagg gaaaggaaga
    41581 gactatgttt tatcgattta tgcgcggggt agttcgagta ctattagcct tagttaatgg
    41641 aaatgcccgc tatgagaata aagaagtttt accgcaagat gagaattaca tactagttgc
    41701 tccccatcgt acttggtggg acccattata tctggcggta gctgcagcac ccaaggaatt
    41761 cagctttatg gcaaaagaag aactgttcaa aaatccagtt ctgcgattta tattaactcg
    41821 atccaatgct tttccagtta aaagagaaaa tccaggacca agcgtcatta aaacacctgt
    41881 aaagattttg aaatcgacaa atttgagttt gatcatgttt cccagtggga caagacatgc
    41941 tacagaactt aaaggcggaa tggcattgat ttccagaatg tctaaagcga aaatcgtgcc
    42001 agctgtatat caaggtccgt tgacacttgg ggatctgttc aagcgtcaga gagtgacagt
    42061 gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa acggataaag aaggtatggc
    42121 ggagatcgaa cgacgaacac aagcagcttt tgacaaattg gacaaagaag tgaaccctga
    42181 tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc ggtcttttga
    42241 atctactgtc ctgttcagtt cagacttttt caatgaaaac gagggagaga cgtcaagtct
    42301 gccctctttt tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag
    42361 tgttataata attagaagga cattttttga ggtgattgaa tgcgtttttt acatacagcc
    42421 gattggcata tcggaaaaaa actacaaggt tatgacttga ttgaagacca gcgtcatgta
    42481 ctatcttcta tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga
    42541 gacttatatg ataggagtgt cccagctgtt gatgccgtag aattatacaa tgaattgatg
    42601 gtcgattgga atttaaagga aaagatcccg atttttgcta tatcaggaaa tcacgatagc
    42661 agtacgagac tttcagcggg tacgccatgg ttcagttatt cgaactattt tttgcgaaca
    42721 cagctagcag aagcatttga accagtcgag tttaaaaacg tacaattttt ctttcttcct
    42781 tactttgaac caattgcagc gagattatat tttgaagatg acaatatccg aacgatcaag
    42841 caagcggtag ccaaggtgat agataaaatg caggagctat ttgattcaga aaaaaaacaa
    42901 gtattgatta gccatttttt tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc
    42961 gaagtaggcg ggttggattc gatccctggt gaactactta caccgtttga ttatgtagca
    43021 ctaggacatt tgcataatca agctgctttg agacaggata atgctcgcta tagcggttcc
    43081 ccattgaaat tctctttatc tgagatcaac caaaaaaaag gcgtatggat cgttgacctg
    43141 acaacaggcc tggagttgaa ttttagggag atcgaacccc tgcatgatat tgaagaaata
    43201 acagcgagtt tcaaagaatt gctggatcct gccttttatc agtccgtcaa tcgggagaat
    43261 tacatctata tctatctcac tgatagaacg atcatcccga atatgatgaa ccagcttcgg
    43321 caaatatatc ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag
    43381 cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat tagcagaagc attttttagt
    43441 gaaatgacgg aagagcagct aactgcaaac caagaaaaat ggctcggaga aacgttgcaa
    43501 gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa aattacaact taaaaatttt
    43561 ggtcctttta tcaatgagac cgtagatttt tcacgtttga cagaagcacc gctttttttg
    43621 atcagcggga agacaggagc tggcaaaacg acgatttttg atgggatcac cttcgcactt
    43681 tttggtgaaa cttccggaag acttcgctct ggaaaagaga tgcgttcttt gtttgctaca
    43741 ccagacgaag aaacaagcgt gactttttct tttgaacatc agcagatgag ctatgaaatc
    43801 gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca
    43861 gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa
    43921 cgtacagaag cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa acagttttct
    43981 caaatcgttt tactacccca aggagaattc cgtaattttt tggtttcatc aagcagtgat
    44041 aaagagactg ttttacgtca tttattcggt actcaatttt tccaacagtt caatgagcga
    44101 ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc atttagagca agagctgcat
    44161 cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc
    44221 gttttagccc aatggacaaa ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa
    44281 gaagctctaa cggaacttca ggaaaaacaa aagcaactag aaaattccta ctatcgtttt
    44341 gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa taaacaagca aaaggaatta
    44401 gctgctcaaa aagacgaaat agaggaaaag aaacgatgga ttgggtatta tgaatttacg
    44461 caacgattag tcgaaccaat ccagcgccta caagaaataa aagttgaaaa aatagaactg
    44521 tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag
    44581 tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa
    44641 aaactgcaaa acaaacagat gatggtgcca attgtcgaat ccttacgtag taaaaaacaa
    44701 gacgcagaac aattaacaat ttcattggat aaagaagaat tgaatgccga gaagctgtat
    44761 caaaatctcg ctatctatca ggaaagaact cgaattcttc aagaaaattt actggatcag
    44821 gaaaaactgc aagaagatcg tcttgctttt ggacagttaa aatatctaga tgaaaagcag
    44881 caagaaatag aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat aagagaagaa
    44941 ctcaaaaaag caactgaaga aataacaaaa aatagtcagc tattgaaaga acagacagaa
    45001 caagcagaac agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt
    45061 ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg aacaccccat acaacaatta
    45121 aaacacgaac agccaaataa ggaagatatc ttaaaagtag aacaacaact ggcagaagct
    45181 gagtctgctg tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat
    45241 cttcaacaac aattatcaga tttagaacaa aaaatcatgg caacgaatat acaattggct
    45301 gaacaagaag aacacttgca acaggctttg aaaaaggcat ttcctgagat aacagaacaa
    45361 aaaacaaaaa caactcttgt agtaatcgaa caatctttag atagacgaaa aagcaagatg
    45421 gccaatgcaa gaatagagct ggaagaaata agacaacaag cagaaaagct cgaacaacaa
    45481 ttagctgtct acaaacagca gcagacagaa aaaaataatc agctccagca actgcaagga
    45541 gaaatcaaaa gtttagaaca acaattgcaa gatacggata gtaactgttt agaagaggag
    45601 atacgacgac ttgaaaaaca gttagaagaa gactcgaatc aactagctgt tgagcagaaa
    45661 gcaggagtgc aattgttgca agatcttcat acgcttcgga cacagcttga tcttcaacat
    45721 gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag
    45781 ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa
    45841 gcacaatata aagaaaacca acgtacagtt acttcttatc atgaagagca attagtgaca
    45901 gctgaccggc tgcgtcaatt agaaaatatc cagctaactg agacattccc agatacagaa
    45961 gagatcagcc aacagttaga agaaataaga cgtatgatcg aaataaagca agaaaagata
    46021 tacggtttac aagaacaaaa aattagtaat caaaaaatct acgatgaatg tctttcgatt
    46081 tatcacacaa gccagacgaa attagatgag cttacgcaat tacagcagct ttcgcaaaca
    46141 ttcaacggag aaaatcctaa gaagactagt ttggaacggt atgtgcttca ggtttatctt
    46201 caagaagtgt tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa
    46261 tttgagctag cggataccat tggcagttat cgagggaaaa ccggattaga aatcaatatc
    46321 tatgatgatc aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt
    46381 attgcagcac tttctttagc attagctctt gccgatgtga tccagacaca agctggtggt
    46441 gtagcaatcg atgcattgtt tattgatgaa ggatttgggt cattagatga agaagctttg
    46501 gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag gaagaatgat cggcatcatt
    46561 agtcatgtaa gagaattgaa agaacgagta ttacaacaaa taagaattga tacagaaggt
    46621 agcggacaaa gcaaaatacg ctatcaactt ggataaaaag ggagattggt aactatgaaa
    46681 tggagtattc cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga aggccgagtg
    46741 ttatcggttg tccctgacca agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa
    46801 ttgtatatgg ttgatttgga tggtacagca aaagagatag attactgcca atgtccctat
    46861 tgggaggaac ataaatattg taagcataca gttgctgtgg aactttattt acggtcgaaa
    46921 aatctatctc gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa agcaacaagt
    46981 gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg
    47041 gtgcaacctt tgattattga ataccaagta gaaacgattg agactaatca gtatcaccca
    47101 gagctttcta ttttggcact ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca
    47161 tatatcgtaa aaaacatcta tgatttttta caagcttaca cagaaaaaga aacttacact
    47221 gttaataaac agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa
    47281 atactcacgc atcttgcagg aagtgctcag acgcatcaat tattaggtac gaacggctta
    47341 caagtcaaag gaaaactaga taaaagatat ctgcttctcc cagtagaaca agcccgcttc
    47401 ctgttagagc agatgaatca gactacacgt ttgcatctgc agataaatga acaaaacgat
    47461 caaggaatac aattttccga tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga
    47521 gaagattatt tgctgcaagc attgaatgat tttgattttt tctttatgca ttatcagtgg
    47581 ggagttatcc aagggacatt ttattctctg acgaaatatc aacaaacggt ttatcaaact
    47641 tggaaacaat tgattcgccg attggagaaa ccagaagtag tgttcaagaa aaaagaatta
    47701 ccgtctttgt ttaaagagat tatccctctg ctgtcagaga tcggcgacgt agctattgcc
    47761 cctgcagtag aagaggaagt agcagatatc cccgtcgaat ttattttctt tttccgaaaa
    47821 gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat ctattcgact
    47881 gacccgaagc atgaggtctc gacagaacag gcttttcagg tgttacgtga cacagtagaa
    47941 gagaaacgag tacttgatct attcagaatg tatcgctacc agaaaaatga aactggttat
    48001 gaacgtccat tgccaagcgg ggaagaacta tatgcctttt tccgcacaga actatctgtc
    48061 ttccgtcagt ttggtgaagt cagacttgga agaaaattgc aagagttgta tttagatgcg
    48121 cataaacatc aaccgaaaat cgaagtaacc gagaccggtt catggctcga tattcgattc
    48181 gatgtgacag ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat
    48241 gatgcttttt atacattaga aaatggtcag gttctttctt ttgattcaga agaatttcaa
    48301 caaacaagcc aagtgttgca acaattacgg gaatcgatac gtacagaaga aggaacgatc
    48361 catgtaccta aaaatcaagg attgatcatt caaaatcaat tggaaaaaag caatgctact
    48421 tttagtgaat cgttccaaac gatggtacaa gatctcattc atccagaaag ataccaggca
    48481 cagcttccca aaggattgaa tgcaacgatg agagattatc aaaagcaagg ttttcgatgg
    48541 ttgaagatgt taggtcatta tcagtttggc ggtattttag ccgatgaaat gggacttggg
    48601 aaaacactac agacaattgc ctttttactt tcggaaaaag aagaacggaa aagcttctct
    48661 gctttgatcg tagcgcctgc gagcttaatt tacaactggc aggcagaggt gagaaaattc
    48721 gccccttctt taagtatcca agtaatcaat gggaataaaa aagaacgtga agaattactg
    48781 gctaaagaca cagatatccg agtgacttct tatgccagct taagacagga tctagctaat
    48841 tatcagtcgc aaaaaattga ttatttgatt cttgatgagg cacaaatggt caaaaatagc
    48901 agtacgaaaa ctgcacaagc attgagagaa ttagctgtac ctcagcggtt tgcactgagt
    48961 gggacaccaa tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac gattatgcca
    49021 ggatttttcc cgactaaaac aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg
    49081 attcgaccgt ttattttacg gcgagataaa caaactgtgt tgaaagactt gccggaaaaa
    49141 acagagatga atctatattc tgcacttaca gaagaacaaa aaactgttta tttagcttac
    49201 ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg aagcattcaa gaaaaaccgg
    49261 atcgggatac ttgctggtct gacaagactg cgacaaattt gctgcgatcc tcgtctgttt
    49321 attgaagatt accaaggcgg ttccggcaag ctcgaacaag taaaagatct actggtagct
    49381 gccaaagaga acaaacgaag agttttgctg ttttctcaat ttacagggat gctgactatc
    49441 ttgcaagaag agttagccga gctagggatc tcgacatttt atttaagagg cagtacgaaa
    49501 ccacaagatc ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg
    49561 atttccttaa aggctggggg aactggattg aatctcacgg gagcagatac ggtcatcttg
    49621 tatgaccttt ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc acatcggatc
    49681 ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg aaggaacgat tgaggaacgc
    49741 atggattcac tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa
    49801 gaacagttga cgaaactgac tgaagaagac atccgcatga ttttaagtgt aggagaaatc
    49861 gaggagtaag agactcgaaa aagcgttcag taatgggtaa gaaagccttt ctaaagaagc
    49921 gattgtttat ttgatttaaa aaatagcaag gaggctggga ttccatttta tcccagcttc
    49981 cttgctatca tttttttatt tctattttta aaggttatac aaagattgt
//
LOCUS       DAZUMM010000009        47695 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000009 DAZUMM010000000
VERSION     DAZUMM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.009"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..540
                     /locus_tag="ACG8E6_000473"
     CDS             97..540
                     /locus_tag="ACG8E6_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS5060921.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(546..1730)
                     /locus_tag="ACG8E6_000474"
     CDS             complement(546..1730)
                     /locus_tag="ACG8E6_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFS5060922.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            1998..2696
                     /locus_tag="ACG8E6_000475"
     CDS             1998..2696
                     /locus_tag="ACG8E6_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS5060923.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2703..3380
                     /gene="nth"
                     /locus_tag="ACG8E6_000476"
     CDS             2703..3380
                     /gene="nth"
                     /locus_tag="ACG8E6_000476"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFS5060924.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3506..4039)
                     /locus_tag="ACG8E6_000477"
     CDS             complement(3506..4039)
                     /locus_tag="ACG8E6_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060925.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4336..6717)
                     /locus_tag="ACG8E6_000478"
     CDS             complement(4336..6717)
                     /locus_tag="ACG8E6_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS5060926.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGAGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6707..7354)
                     /gene="recU"
                     /locus_tag="ACG8E6_000479"
     CDS             complement(6707..7354)
                     /gene="recU"
                     /locus_tag="ACG8E6_000479"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFS5060927.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7418..7957
                     /locus_tag="ACG8E6_000480"
     CDS             7418..7957
                     /locus_tag="ACG8E6_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFS5060928.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8047..8466
                     /gene="gpsB"
                     /locus_tag="ACG8E6_000481"
     CDS             8047..8466
                     /gene="gpsB"
                     /locus_tag="ACG8E6_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFS5060929.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8488..8855
                     /gene="rnpB"
                     /locus_tag="ACG8E6_000482"
     ncRNA           8488..8855
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8E6_000482"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9064..10230
                     /locus_tag="ACG8E6_000483"
     CDS             9064..10230
                     /locus_tag="ACG8E6_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFS5060930.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10276..11772
                     /locus_tag="ACG8E6_000484"
     CDS             10276..11772
                     /locus_tag="ACG8E6_000484"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFS5060931.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11853..12536
                     /locus_tag="ACG8E6_000485"
     CDS             11853..12536
                     /locus_tag="ACG8E6_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5060932.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            13181..14371
                     /locus_tag="ACG8E6_000486"
     CDS             13181..14371
                     /locus_tag="ACG8E6_000486"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFS5060933.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            14473..15426
                     /locus_tag="ACG8E6_000487"
     CDS             14473..15426
                     /locus_tag="ACG8E6_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFS5060934.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            15495..16844
                     /locus_tag="ACG8E6_000488"
     CDS             15495..16844
                     /locus_tag="ACG8E6_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5060935.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVIAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(17027..17350)
                     /locus_tag="ACG8E6_000489"
     CDS             complement(17027..17350)
                     /locus_tag="ACG8E6_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060936.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            17479..18642
                     /locus_tag="ACG8E6_000490"
     CDS             17479..18642
                     /locus_tag="ACG8E6_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS5060937.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            18712..20061
                     /locus_tag="ACG8E6_000491"
     CDS             18712..20061
                     /locus_tag="ACG8E6_000491"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS5060938.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(20106..20537)
                     /locus_tag="ACG8E6_000492"
     CDS             complement(20106..20537)
                     /locus_tag="ACG8E6_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5060939.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            20925..23567
                     /gene="alaS"
                     /locus_tag="ACG8E6_000493"
     CDS             20925..23567
                     /gene="alaS"
                     /locus_tag="ACG8E6_000493"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFS5060940.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            23767..24363
                     /locus_tag="ACG8E6_000494"
     CDS             23767..24363
                     /locus_tag="ACG8E6_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5060941.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            24485..25633
                     /locus_tag="ACG8E6_000495"
     CDS             24485..25633
                     /locus_tag="ACG8E6_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5060942.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(25671..26084)
                     /locus_tag="ACG8E6_000496"
     CDS             complement(25671..26084)
                     /locus_tag="ACG8E6_000496"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFS5060943.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            26163..26594
                     /locus_tag="ACG8E6_000497"
     CDS             26163..26594
                     /locus_tag="ACG8E6_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFS5060944.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(26693..26926)
                     /locus_tag="ACG8E6_000498"
     CDS             complement(26693..26926)
                     /locus_tag="ACG8E6_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5060945.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            27109..28779
                     /locus_tag="ACG8E6_000499"
     CDS             27109..28779
                     /locus_tag="ACG8E6_000499"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFS5060946.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            28926..29312
                     /locus_tag="ACG8E6_000500"
     CDS             28926..29312
                     /locus_tag="ACG8E6_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060947.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            29299..29997
                     /locus_tag="ACG8E6_000501"
     CDS             29299..29997
                     /locus_tag="ACG8E6_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFS5060948.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            30002..30466
                     /gene="lspA"
                     /locus_tag="ACG8E6_000502"
     CDS             30002..30466
                     /gene="lspA"
                     /locus_tag="ACG8E6_000502"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFS5060949.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            30463..31374
                     /locus_tag="ACG8E6_000503"
     CDS             30463..31374
                     /locus_tag="ACG8E6_000503"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS5060950.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            31609..32148
                     /gene="pyrR"
                     /locus_tag="ACG8E6_000504"
     CDS             31609..32148
                     /gene="pyrR"
                     /locus_tag="ACG8E6_000504"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFS5060951.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            32162..33445
                     /locus_tag="ACG8E6_000505"
     CDS             32162..33445
                     /locus_tag="ACG8E6_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFS5060952.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            33458..34384
                     /locus_tag="ACG8E6_000506"
     CDS             33458..34384
                     /locus_tag="ACG8E6_000506"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFS5060953.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            34403..35683
                     /locus_tag="ACG8E6_000507"
     CDS             34403..35683
                     /locus_tag="ACG8E6_000507"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFS5060954.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            35688..36764
                     /locus_tag="ACG8E6_000508"
     CDS             35688..36764
                     /locus_tag="ACG8E6_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFS5060955.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            36769..39954
                     /gene="carB"
                     /locus_tag="ACG8E6_000509"
     CDS             36769..39954
                     /gene="carB"
                     /locus_tag="ACG8E6_000509"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFS5060956.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            40008..40784
                     /locus_tag="ACG8E6_000510"
     CDS             40008..40784
                     /locus_tag="ACG8E6_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFS5060957.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            40781..41713
                     /locus_tag="ACG8E6_000511"
     CDS             40781..41713
                     /locus_tag="ACG8E6_000511"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFS5060958.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            41710..42426
                     /gene="pyrF"
                     /locus_tag="ACG8E6_000512"
     CDS             41710..42426
                     /gene="pyrF"
                     /locus_tag="ACG8E6_000512"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFS5060959.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            42433..43062
                     /gene="pyrE"
                     /locus_tag="ACG8E6_000513"
     CDS             42433..43062
                     /gene="pyrE"
                     /locus_tag="ACG8E6_000513"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFS5060960.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            43050..43364
                     /locus_tag="ACG8E6_000514"
     CDS             43050..43364
                     /locus_tag="ACG8E6_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5060961.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            43402..44043
                     /locus_tag="ACG8E6_000515"
     CDS             43402..44043
                     /locus_tag="ACG8E6_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFS5060962.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            44086..44778
                     /locus_tag="ACG8E6_000516"
     CDS             44086..44778
                     /locus_tag="ACG8E6_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFS5060963.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            44775..45677
                     /locus_tag="ACG8E6_000517"
     CDS             44775..45677
                     /locus_tag="ACG8E6_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5060964.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(45835..47541)
                     /gene="efbA"
                     /locus_tag="ACG8E6_000518"
     CDS             complement(45835..47541)
                     /gene="efbA"
                     /locus_tag="ACG8E6_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFS5060965.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        15520 a         8351 c        10270 g        13554 t
ORIGIN      
        1 tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa taggaaacaa
       61 aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca actgctaata
      121 gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga aatggaaaaa
      181 gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg gaaaatccct
      241 gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc catcagtaag
      301 ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga agagaataaa
      361 aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag acacgagcaa
      421 ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc aaaagaagaa
      481 tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag tagcaaatga
      541 agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa tccgaatacc
      601 gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc gatgacgcgc
      661 gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt atctgagaca
      721 gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa accataacag
      781 ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt gatgaataga
      841 taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga aaattttttg
      901 agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat caaagagaag
      961 cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg agaaaagata
     1021 gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa aggaataacg
     1081 gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga gaaacttaaa
     1141 tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc ataaacggca
     1201 tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa ctcagctaat
     1261 agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc catccatcca
     1321 aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata taaggtggag
     1381 atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca tatcgaattt
     1441 cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat aaggatcgga
     1501 tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa ggcagctaga
     1561 gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga gtgagaataa
     1621 ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc agtgaataag
     1681 acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat gtaaaaactc
     1741 ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt gtttccttgt
     1801 caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag tgaagatacg
     1861 catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg accgatcagt
     1921 gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt atactgtgat
     1981 tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga caaacagtca
     2041 tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa gaatttttat
     2101 ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat ttgcagatga
     2161 ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat cgtctcgtaa
     2221 caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca gatcgatata
     2281 atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa aaaaaatacg
     2341 aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa caagaatttg
     2401 gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa gaagaccatt
     2461 atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag gcttatagtt
     2521 ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt acaaaacaac
     2581 aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa gtagaacagt
     2641 cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag gagtagcgga
     2701 atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga atgtttcccg
     2761 aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca gtgatcttaa
     2821 gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt gcctcttttc
     2881 cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa ataaaaacga
     2941 ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag ctgattgaac
     3001 gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct ggtgtaggca
     3061 gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc gcagtagata
     3121 cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct acggtcatgg
     3181 aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag acacatcata
     3241 cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc gaggtatgtc
     3301 ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa aaggcgctaa
     3361 aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta tgaattatct
     3421 tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt aaatgtacat
     3481 actcagtagc taggatggta acttattaca atttattgcg ataaaaataa aagttgttat
     3541 ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct ataaaataag
     3601 tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt gtaataaaaa
     3661 tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg gatttgggcg
     3721 gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa aaagtgagaa
     3781 tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt aaaagtacag
     3841 aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat aataaacaaa
     3901 atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa aaaagatggt
     3961 gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag ccttctttca
     4021 aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg ggattattaa
     4081 gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata aacaagtaaa
     4141 tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta agctaaatgt
     4201 tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac agcttggtac
     4261 atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac gcttgtagac
     4321 gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg gtgtatggtc
     4381 atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag gagcagatga
     4441 agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag acgctggctc
     4501 gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc tttgactagt
     4561 agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct ggacatagct
     4621 ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat attgatcttt
     4681 gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt ctgtatttgt
     4741 cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta cgtgtgacga
     4801 ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa tcgaataatt
     4861 tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac caagttttgc
     4921 atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata acccagcgat
     4981 ttgcgcattc gtaccagctc cttcagtgat cgtatctttt aaaatgtctg tgatcatata
     5041 agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt ctgttccgtc
     5101 ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat tggcgaaagc
     5161 cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat cttgttcttc
     5221 cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc caagattttt
     5281 taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg ctggaacatt
     5341 acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat tatcccagtt
     5401 tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag tttttccagt
     5461 agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca tcgtagaacc
     5521 aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt ctgcgatgtg
     5581 acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg tggaggcaac
     5641 ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat catatagttt
     5701 cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt agacattctt
     5761 atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga cgatcttagt
     5821 attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat taacagcttg
     5881 atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa cagtatctcg
     5941 tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg cttgaggcat
     6001 cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac caaagtacat
     6061 ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat ttacgtagta
     6121 ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc acgcttcttg
     6181 ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa gtttgatcaa
     6241 ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag agagggcaga
     6301 accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat cttctaccga
     6361 cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt cacgcttttc
     6421 tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag atacggtagc
     6481 gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga aaagtcctac
     6541 acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct tgataaataa
     6601 tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct tcttcacact
     6661 ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat ttgccattct
     6721 tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc aaataaggaa
     6781 tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa gataaaggta
     6841 gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga acactattca
     6901 aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc ccattttgtt
     6961 cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag ggaaaagatg
     7021 ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag acgccattat
     7081 agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca cttctcttag
     7141 gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc gccaacccgc
     7201 gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc ccccgattgc
     7261 cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag gcattcttat
     7321 aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct aggccacatt
     7381 atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa aagtaatcta
     7441 tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc ctaaaatcca
     7501 agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag ggctggaatg
     7561 ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag tagcggaatt
     7621 gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg attttggtaa
     7681 taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag cagattatgt
     7741 cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc acactcgttt
     7801 tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc ctggaaaaac
     7861 gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg aaattcggct
     7921 gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt tgatttatca
     7981 acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa aactagaggt
     8041 gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga attcaaaaca
     8101 aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat catcaaagat
     8161 tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg attgagtgca
     8221 aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca acaaacagag
     8281 gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc aaatcttgaa
     8341 cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc agcacaacct
     8401 aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa aacacgtcaa
     8461 ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga cttgatcagg
     8521 ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc ttagcgaaac
     8581 cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct tcggctatgc
     8641 ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat ccggtggaac
     8701 gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt gtctggaaga
     8761 agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg ttttttctcc
     8821 ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag ggaattaggt
     8881 cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta tttttcaata
     8941 cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta ttcggaattt
     9001 gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat aaggagattg
     9061 tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg gctagaagca
     9121 ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa cggacgtgca
     9181 cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg tacagcagat
     9241 cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt gtttgaaaac
     9301 gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc tgtagctgga
     9361 aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac aaaaaaagca
     9421 atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact gacggaaact
     9481 ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt aactctggat
     9541 acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg agcaccattg
     9601 aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga ccgtccattt
     9661 tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat aggccataat
     9721 atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt cagtcaagaa
     9781 atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat tgaattagat
     9841 attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa tgcagaagaa
     9901 atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt caaaacggat
     9961 aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg agaagaagaa
    10021 actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa aacatggagt
    10081 aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa agcaaccaaa
    10141 aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg gggaactcgt
    10201 ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc aattggatgt
    10261 ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga aatcaatctt
    10321 ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc tgaaaaagct
    10381 agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc aaaaaaaatc
    10441 ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga attgtcggaa
    10501 gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca tcgcgtaccg
    10561 gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca atggcaaaaa
    10621 gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa tattgaatta
    10681 acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga tgttttgctg
    10741 aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga tcagcttaaa
    10801 gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc tgaaacagac
    10861 tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa agtaatcgaa
    10921 caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc atttatgact
    10981 ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga tttcaaaatg
    11041 gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga catcgatgat
    11101 aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga atcacaatcg
    11161 ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca atatcctttc
    11221 tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt ttatcgttca
    11281 ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac ttaccctctc
    11341 catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt agatgttgct
    11401 gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg accaacaaat
    11461 gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg ttattttccc
    11521 tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca gcaagaaata
    11581 gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg gatgtcagag
    11641 catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga tgcaacacat
    11701 gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt taatgtatat
    11761 caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct atagatgttt
    11821 tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg atcgctgatt
    11881 atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat accaaaagag
    11941 ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc tttgtgacgc
    12001 ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat gagaacgagg
    12061 caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa cagatctcga
    12121 tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa gcggccgaac
    12181 tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta ggagcaatcg
    12241 gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt gatcctgaac
    12301 tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca gtgatcaatc
    12361 atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac ggatatgaag
    12421 aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt ctggaatggt
    12481 ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa aaatgatgga
    12541 ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta aaagttactt
    12601 ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt cacaaggaac
    12661 aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt cggaattggc
    12721 aggagaacac cgtttattcg gaagaatagg actgtgacaa gacttttgtc acagtccttt
    12781 tttcgaattc aacaagtaaa acttttaaga aatattcaga gagtaaaatt aaaaattgct
    12841 gataaacgaa gatggaaatt ttatgtattt ttattggtct atgcaataaa taaattaaat
    12901 attcagaata caattgattt taacgaaagg gcttctatca atggttgtgt tgtatttatc
    12961 tgcccaggta gagtaaaata gatcattatc taaaaggtaa ttgttcatac tatataagtc
    13021 atttgaaagt aaaaagatac tgtaaataaa aaatgtggtt aaataattct cataatcagt
    13081 gtaagcgtta tcaaaaatat agcatacttg ctttgtagat gaatgacacg atatatgttt
    13141 ggtatgttct gcaagacaga tgaatatgga ggtggagaca atgtcttcca aagaactaac
    13201 aatgtattta gagaaagaat tggatcaact gaaagaaaag gggttataca atacgatcga
    13261 catactagaa agtgaaaatg gtgcttgtat catcgtagac ggaaaaaaga tgatcaatct
    13321 agcatccaac aattatcttg gatttgccaa tcgcgaagaa ttgaaaaaag catgtattga
    13381 agcaaccgaa acttatggtg taggagcagg agcagtccga acgatcaatg gttcgctgaa
    13441 aatccatcag caattagaag aaaaaatcgc agaattcaaa gggacagaag cagcaatcgc
    13501 ctttcaatct ggattcaact gcaatatggg cgctatctct gcagtaatga caaaagaaga
    13561 tgctatctta tcggatgaat taaaccatgc atctattatt gacggttgcc gcttatccgg
    13621 agcaaaaatc atccgaatta aacatcaaga tatgaaagac cttgagaaaa aagcaaagga
    13681 agcgatagaa agtaagaaat ataaaaaaat catgtatatc acagatggtg ttttctccat
    13741 ggatggagac attgcacgat tgccggaaat tattccgatt gttgaaaaat atggactgat
    13801 cacctacgtc gatgatgcgc acggctcagg cgtaactgga aaaggtgcag gaaccgtcaa
    13861 gcattttgga ttaagcgaca aaatcgatat gcaaatgggc acgttatcga aagctatagg
    13921 tgttgttggg ggttatgtag ctggatcgaa aacactaatc gactggttga aagctagaag
    13981 tcggccattc ctattttcta cttcgctaac tccaggtgca gctgcagctg cattggcttc
    14041 gattactttg atgcaagaac atccggaact tgtcgaaaaa gtttgggaaa atgctaatta
    14101 ttttaaagaa gaattgaaaa aagtaggcta taacatcggt atgtctgaaa cacctattac
    14161 tccagtcatt ttgggagacg aaaaagtgac tcagactttc tctaagaagc tgatcgaaca
    14221 tgggatttat gctaaaccaa tcgtttatcc aactgtgcct cttgggacag gcaggatcag
    14281 aaatatgccg actgcggaac atacgaggga aatgcttgat gaagcagtag cagtttatca
    14341 aaaaatagga aaagaaatgg aaattattta actaaattta gttttttcgt ctatttaaaa
    14401 ttagagaaca tgttcacgca tatattgtta atcatctgaa gatagtagta aaaaagatca
    14461 gggaggatca tcatgaaaaa agtattggtg acaggatgtt taggacaaat tggttcagag
    14521 ttgatcacac ggctacgtca agaactaggc agtgaaaatg tcattgcgac agatatacgc
    14581 atgcctgaag tcattgagaa aaacgatatc tttgagatcc tagatgtaat ggattacgaa
    14641 aaaatgcgca gcatagcaga acaatatcaa gtggacacat tgattcattt ggctgctctt
    14701 ttgtcagctg ttgcagaagc aaaaccacaa ttagcctgga atttgaacgt gactgggctg
    14761 gtcaatgcat tggaagtcgc tagagaattc cagctgaaat tttttacgcc aagttcaatc
    14821 ggcgcgtttg gtccaaatac tccaaaagat cataccccac aagatactct tcagcgtcct
    14881 acaaccatgt atggggtaac gaaggtagct ggagaactac tatgtgatta ttattatgtg
    14941 aaatatggcg tagatactcg tggtgtccgt ttccctggat tgatttctta tgcaaccttg
    15001 ccaggaggcg gtacgacaga ctacgcagtc gatatttatt attcagcaat caaagaagga
    15061 aagtacacta gtttcattga tcaaggtaca tcaatggata tgatgtatat gccagatgcg
    15121 atcgatgcca ttataaaact catgaatgca gatcctgatc gattggttca tcgaaatgcc
    15181 ttcaatatat cggcgatgtc ttttgacccg gaagagatca aagcatctat tcaaaaaatc
    15241 ataccagatt ttgaaatgga ttatgatgtg gatcctgtac gtcagtcaat agcagaatct
    15301 tggccaaatt cactagatgt ttcttgtgcg caagaggaat ggggcttttc gccgaaatac
    15361 gacttagatg tgatgacaaa ggatatgttg gaaaaactga aagaaaaatt ggtagagacg
    15421 gtataaaaca agaaccacat ccaaaaagtt ttttatactt ttgggattgg ctatggtatg
    15481 aaaggggaca agggatggaa gaatatcaag ccacgccagt aaaagaagta aaaatagaaa
    15541 atgaattaaa acgtacgatg ggatttttta ctgctttatc gactgttatg ggcacagtga
    15601 tcggtgcagg ggtatttttt aaagccgcaa gcgtagcaga agttacagga tccgccagtt
    15661 tgcatatgtt ttcttggttt ctcggtggta tgatttctgt ctgtgcagga ctaacaggag
    15721 ctgaactggc agctgctatt ccagaaacag gagggatgat taaatatatc gaacggattt
    15781 acggaaatac agcagctttt ttattgggct gggcgcaagt cgtgatttat tttccagcaa
    15841 atgtagcagc cttgtcgatc attttcggta cacagttcgt caatctattt ggtctttccc
    15901 agtcaatgat tgttcccgta gctgttacag ctgctgtatc gatcctgttg atcaattttt
    15961 taggttctaa agctggaggg gcttttcagt cgattacgct tgtctgcaag cttattcctt
    16021 tgtttgtgat cgtcattttc ggtttgttcc gacaagaagg agtagacttc caactgtttc
    16081 cgatccaagc aggagaaaat ctttcatttt tctcagcctt gggagcgggc ttgctagcaa
    16141 cgatgttcgc atacgacggt tggatacacg taggaaatat ttcgggagaa ttgaaaaagc
    16201 cggcgaaaga tttgccaaaa gcaatttctt taggaatcat cggaatcatg atcgtttatt
    16261 tattagtaaa tgcggtgttc ttaagaaccg catctatcga cggagtcgca ggaaacagca
    16321 atgcggcaag tgacgttgca aaaatgattt ttggcggctt cggtggaaga ctggtcacag
    16381 ttggtatctt gatttctgtt tatggaacca tcaatggcta tacccttact ggaatgcgtc
    16441 tcccttatgt tattgcaaaa gaaaacaacc tgccttttag caagctcttt gctaaacttc
    16501 atgataaaac taaagttcct gtagccgcag gtatcttaga gctagtcatc gctattggca
    16561 tgatgatgat cggtggtttt gatacactaa cggatatgct gatctttgtc atttggattt
    16621 tttatacgat ggtctttgtt ggtgtcattc ttcttcgtaa aaaagaacct gatttgtttc
    16681 gaccatacaa agttccaatg tatccgttta ttccactagt agcaatcatt ggagggactt
    16741 ttatcgtatc aagcacgctt atcactcaga cctttttagc ttcgatgggg atagtattga
    16801 cacttgctgg tatcccgatt tatctctatt taaaacgtag atagctaaaa cattcgaatt
    16861 ttattagaac tgcacttctc tgccaaacaa aatattttat ttaaatgagg agtacgtgga
    16921 agaaatgtca aaagtactcg ttaattttga tcacgtaaat gtcgttttgt ctacgatgaa
    16981 atagcaaagt aaaagacaga aatcgggctc aaccgatttc tgtcttttac tttgatttgc
    17041 gtaactgaac tattcggcgt acgccgcgat aaatatttaa taaaaccaag caaccagaaa
    17101 tgaaaatggg aagccaaatc agatagacag ggtagccaag cactttagac aaagcatgtg
    17161 gagtcatttt atccatttca agaagtgctt gctgccaagt ctgaagattg aagactagca
    17221 tgaaacccga aataatcagg agaataaatc ctaaataatg aattgtagtt ctttttctta
    17281 cataagcgat cgtagcataa aggaacagta aaaaaatagg tacgccaaaa acaaagtaca
    17341 tattcagcat ctcgacaccc cctttgtgtt tatcttactt tactttgaaa gaaatgagaa
    17401 ataatagcct cgttacttgc ttgaaacagc aaaggactgc taagataaga gagatacaaa
    17461 gaatcaagga ggagtcatat ggcaagttat atcgaaccga ttcgttttgc gctgattgtt
    17521 tttccatttt tagccttagc gatctcatct gtttttttta tttatgaata tcgaaagtat
    17581 ggaacatttc tagtaacaag ggcagttatt ttgtattctt tcgtttttta tttattgtgt
    17641 gcgtattttt tggtgatctt accacttccg gcaaaggaga cggttgccca aatgacaggg
    17701 ccaaagatgg agctgcattt atttgcttca tggcaacatt tcagagacaa cacggtattg
    17761 gtcttgtctg aacctagtac ctacctgccg gcaatgaagc agagtgtatt tttggaacca
    17821 gcttttaatg ttttattact cataccattt ggcatttatt taagatatta tttccggctt
    17881 tcttttatga aaacgatgat tgctagtttc tgcctttctc tattttttga attgacgcag
    17941 ctaagtggac tttattttat ctacccaaga ccataccgct tatttgatgt gaatgattta
    18001 gtgacaaata cattgggtgg aatcattggt tttgtcgtta cgccgttatt tacatttatg
    18061 cttcctacaa gaagacgaat ggatgaactt tcttatgaaa aaggaaagac tgtctcattg
    18121 actcgacgct tggtcgctta tttgatcgac tggacatttt tatctcttat taatttggta
    18181 gcggcgattt tctttaaaat tgcgttcgat tcaggggcag ttacttctca tatctggtgg
    18241 cttttattag aagtaggggt atactttatg attgtaccgt ttataaccaa tggatggaca
    18301 cttggcaaaa aaattgtgcg tatccgtttg gtggaagaag accgcgaacg aatttcttta
    18361 aaagcattga tgatccgcta cgggtatctg tactttcttt tttatggttt aagctggttg
    18421 gggcagttgc ttcagcaaat catgaattcg acaacgaata cggtgttgca gatgagtgca
    18481 gtattcattt ctctcggtgt cttgttacta caaggtattt ttattttaaa tatcttgcta
    18541 tcatttttga gaaaaaaacg gattctgttt tatgaaaaag ctagtcacac atatactgtt
    18601 agcacgattg gccaaacaaa ggatgatgat tctcgaaatt agctgagcaa aaacgctttg
    18661 tctgtgttat aatgccaaag tcgtgataat ttaaagagaa gaggtgcacg aatgaataat
    18721 tttgatatat tccgcttcaa accgtttatc aatgaagcgt taaccgcaaa gggcttcaaa
    18781 cagcccacag aggtccagga acgtttgatt cctttaattg caaaaggaaa aaatatcatt
    18841 gggcaatctc aaactggtac aggaaaaaca catacgtttt tgttgccttt gtttgaaaaa
    18901 atcaatgcag aaaaagagga agtacaagtt gttattacag ctccaagtag ggaactagct
    18961 gcacagatct atgaagcagc tacacaaatc gctaaatttt ctacaccgga gatccgtgtc
    19021 tcgaattttg tcggcggtac agacaaacag cgtcaattaa atcgtctgca tcaccaacag
    19081 ccacaggttg tagtaggcac gcctggtaga atattagaca tgatgaatga acaagcgctg
    19141 aagattcaca ccgcatttgc atttgttgtc gatgaagcgg atatgacatt ggatatgggt
    19201 tttttaaatg aagtagacca aattgcaagt cgattaccag aaaagctgca gtttttagtt
    19261 ttttctgcca ctattccaga aaaacttcgt ccattcttac gaaaatacat ggaaaatcca
    19321 atcattgaag aaatcaaacc aaaagcagtt atttctgaag acattgaaaa ttggctgatt
    19381 tcaacaaaag gtaaagatga gaatcaaatg atttatcaac tgctgacaat tggtcatcct
    19441 tatttagcaa tcgtatttgc caatacaaaa cagcatgtgg atgaaattgc cgattacttg
    19501 aaaaaccaag gcttgaaagt agcaaagatt catggagaca tcaccccaag agagagaaaa
    19561 cgagtgatgc gacaagtaca gaatctggag tatcaatatg ttgtagcgac tgatttagct
    19621 gctagaggga tcgatatcga aggtgtctct catgttatta atgcagaaat tccagaagat
    19681 ctagacttct ttatccatcg tgtcggacgt actggacgta atggcttaaa aggaacggcc
    19741 attacgttat attcgccaaa tgatgagcaa gccattgatg cgatagaaaa attgggcgtc
    19801 tcatttgaac caaaagaaat caaaaacggg gaaatcgtca aaacatacga tcgtaataga
    19861 cgtgccaaac gagaaaaatc aaaagatgta ttagatccca cgctgattgg tcttgtgaag
    19921 aagaaaaaga aaaagatcaa gcctggttat aagaaaaaaa tcgattgggc aattgcagaa
    19981 aaaaataaaa aagaacgcaa aatcgaacgc agacagcaaa gccgtactgc tcgtaaaaat
    20041 aagaaaaatt cacataaata aaaaacaaga ggttgtgaca aaaggcgtgt cacaatctct
    20101 tgtttttaag ctagactttt tttcattccg atacaaagta cttcatcttc aataaaaggt
    20161 tctgtgcaag tatgataccc ttgtttttca taaaaatcta cagccgtcat ttcagctact
    20221 acataactag tctgacaacc ttctgaaacc gcccgttttt cagcagcttc aagcaactga
    20281 ccgcctaacc cttttcctcg gtattctttt gctacgcaaa atcgttccgg cttgaagcaa
    20341 gtaggtgttt tcttttcata acgcaaagta gctactggtc ttttttgatc aagaaataga
    20401 taataggttt gttgcgggat atcttttccg tcgaattcta gttctgcagg aatcccttgt
    20461 tctaatacaa aaacttgata tctcaaaaag cagctagctg cgcgtatcca ttcatccgct
    20521 ccaactattt ttttcatcat attcctcctt gtcatgaata gcagagatgt attgattcct
    20581 ttattcttat cgtaccaaaa atcgtggagt atgaccaaaa ggaaatgaaa ataaaaattg
    20641 agatattgac agtgatcaat agaatgcgta tactaattaa gtacgaaaag gatatgattt
    20701 gttttttaag actcacagaa aattctttct tggctgaaaa aagaatagtc actaatcaaa
    20761 ttgctccctt tttctatttt ggtagaaata ccgacgcagc tcagcgttat cgagtttaag
    20821 aggtaatgat tacacatcat tgcaatcaag gtggtaccgc gagttttcgt ccttggctgt
    20881 gatggtgttt ttttgtattt aatttataga aagcaggtaa tgacatgaaa caattaagta
    20941 gtgcacaagt tcgacaaatg tttcttgatt tcttccaatc taaaggacat acgattgaac
    21001 ctagtgcgtc tttgatcccc gtgaacgatc caaccttatt gtggatcaac tctggtgttg
    21061 caacattgaa aaaatatttt gatggttcag ttgtgccaga aaatccaaga atcacaaatg
    21121 cccaaaaatc tattcgaaca aatgatatcg aaaatgtcgg taaaacagca cggcaccata
    21181 cgatgtttga gatgctgggt aatttttcga ttggtgacta tttcaaaaat gaagccatcc
    21241 actgggcatg ggaatttttg actgatccaa aatggatggc attcgatcca gaaaaacttt
    21301 atgtaacagt ttatccaaaa gatacagaag caaaacgcat ctggcatgaa gaagttggcc
    21361 tgccactaga tcacattatt gatgtagaag acaacttctg ggatatcggt gcaggcccat
    21421 gtggtccgga tacggaaatt ttctatgacc gtggagaagc ttataacgat ttagcagaag
    21481 acgatcctga aaactatcca ggaggagaaa atgaacgtta tttagaaatc tggaacttag
    21541 tattttcaga attcaaccat aagcctgata acacatttga accacttcct cataaaaaca
    21601 ttgatacggg gatgggattg gaacgcatgg tctcaatcgt tcaagatgcg tcaacgaact
    21661 ttgagacaga cttgtttatg cctattatcc acgccgtaga aaagctaagc aaacaagtaa
    21721 aatatggtga aaacccagta acagatattt cttacaaagt aatcgcagat catattcgcg
    21781 ccctttcatt tgctattggc gacggtgcat tgccatcgaa tgaagggaga gggtatgtct
    21841 tacgccgttt gcttcgtcgt gctgtcatgc atgggaaaaa attaggtatt gaacaagcat
    21901 tcttatatcg tttagtccct gttgttgggg aaatcatggt aagttattat cctgaagtac
    21961 ttcaacaaaa agaatttatt gaaaaagtta tccgtacaga agaagaacgt ttcaatgaaa
    22021 caatcaatga aggtttggaa atcttaaatc aagtgatcca agaagtaaaa aacgaaaacg
    22081 ggacgactct agcaggaaaa gacatcttca aactatacga cacatatggc ttcccggtag
    22141 aattaacgga agaggtggca gaagatgaag gactgaaagt tgatcatgct ggttttgaaa
    22201 aagaaatgga tgcacaacgc gaacgtgctc gctctgcccg cagtactgaa aaatcaatgg
    22261 gggtacaatc tgctttattg acagatatca aagtagaaag taaattcatt ggatacacag
    22321 aacaagaatc agatagtcaa ttattggtca tcgttaaagg tgagtctctt ttgaatgaag
    22381 tcgcagaagg tgaagctcaa atgatctttg atcaaacacc tttctatgca gaaatgggtg
    22441 ggcaagtagc ggatcaagga tggatcatga atgaagtagg cgaaatcgta gcagttgtta
    22501 cagatgttca aaaagcgcct aatggtcaat ttatgcacac cgtagaagta aaagcaccaa
    22561 tgagtgaagg acaaacctat catctattga tcgatcaatc gatgcgcaat cggatcatca
    22621 aaaaccatac agcaacccat ctactgcaca aggcattgaa agaagtactt ggcgagcatg
    22681 caaatcaagc tggttcttta gtcgctcctg gacatttacg attcgacttt acccactttg
    22741 gtcaaatcac ttcagatgaa ttagctgaaa tggaaagaat cgtcaacgaa aaaatctggg
    22801 aagctatccc agttgaaaca attgaaacag atatcgatac agccaaaaac atgggtgcaa
    22861 tggctttgtt tggtgaaaaa tacggacatg acgttcgagt agtaaatatt ggcggctggt
    22921 caatcgaact gtgcggaggc acacatgtag caaatacaga aaacatcgga atcttcaaaa
    22981 tcgtttctga atcaggaatt ggtgcaggcg tgcgacgaat cgaggctgta acaagtaaag
    23041 aagcgtacga actacttgaa gaagaagaaa aacaactaaa acaagtagca acaattgtta
    23101 aatcacctca attgaaagaa gttgtttcaa aagcggaaca attgcaacag caagtaagag
    23161 agctacaaaa ggaaaatgaa caattagcta gcaagttagc gaatcaacaa gctggtgatg
    23221 tattcaaaga tccagaaacg atcaatggaa aaacaatcat tgcagcacaa gtaaaagtga
    23281 aagatatgaa tcagctgcgt caattagctg accaatggaa acaaaaaaat atttcagata
    23341 ttcttgtttt agcaaacgaa caagaaggaa aagtcagtct gctagcggca atgacgaaag
    23401 aatcaaatga agctggacta aaagctggcg acttgattaa agcaatcgca ccgaaagtag
    23461 gcggtggcgg tggtggacgt ccagacatgg cacaagctgg cgggaaaaat cctgctggta
    23521 ttccagatgc acttaaagct gcgaaagaat gggttgcgca acaatagggc gtttacgttt
    23581 gacagaaaaa caaaagttca aacattgaac gaaacatgat tatgggggcg aagacaaaca
    23641 agtcttgccc tcatactttt ttcaattgga tggcgcatct aatcaaatgg tcggattcgg
    23701 atggtcttta gttgacgaaa gtgacagaaa tttttacgat tagtataaat aaaaaggggt
    23761 gaaaaaatga atagtagaaa taagaccttt cgactagtct tacgtgcgat cttgttagca
    23821 atcatcatcg tacaagcgat ggttccttgg ctggggttta ttccattagg attcattagt
    23881 ttaacaatta tccatatcac agtaattatt gctgcagtag tattaggccc aaaagatggc
    23941 atggtgattg gcttgttttg gggaatcgct acaattgttc gtgcctatgc catgccaaca
    24001 acgccatttg atacattagt gttcacgaat ccgattatct cggtagttcc cagagtactt
    24061 gttggcttag tagcaggctt ggttttccac tggatttatc aaaaaaataa atcaataacg
    24121 atcagctcag cctttgcagg ggtactgggt tcattggtca atacagtctt agtgttagga
    24181 ttcatgggat tattttatac aggtgcaacg gcagatgcct atggtgtaga ctctgctctt
    24241 ttatttaaaa ccttagcagg ggttgcagca atcaatggga taccagaagc aataggtgcg
    24301 ggaatcataa caccattact agcaaaagcg ttatttgcag caacaccgtt gaaaccagaa
    24361 taatgaccat cttttaatag taataggacg cacgatagat agaaaaagga agagaggaga
    24421 ggaaaacaat cgtcctctcc atctttttac ctgagcaaca agcaaactag aaggggtaaa
    24481 aaacttggct aagaaacaag aagaaaaaaa gaaacaaata ccgcaacagc ggacaaaaaa
    24541 aggagtacta gcgggtatct taggattggt tacgaatctg atcttgtttg tctctaagct
    24601 aatgatcggg cttttttcag gaagtgtctc cattatggct gatgcagtga acagtctatc
    24661 agatactgct tcatccatat tgactttgat cggatttaaa attgctgcaa agccagcaga
    24721 tcaggaacat ccttatggac acgaacgctt cgaatatatc agtgggttat ttgtttcgat
    24781 catcattact tatgtaggat ttcaattttt agagtcttct gctgaaaaaa tcatccatcc
    24841 agaaaatgtc tctttaacac ccatcgtctt ttttgtccta atcatttcca tctttttgaa
    24901 atttgcacaa ggcaaaatgt atcaggttat tgcgcaatcc attgattctg aaacattgag
    24961 agctacagca acagatagtt ataatgatgt ctttactaca ctggctgtat tgctttcagc
    25021 agcaatcgaa tgggtaactg gatggcgtat tgatggatat ataggttttg ctttagcatt
    25081 atatatcctt tatagtggat tgacgatgct tcgaaatttt gtatacgaac tattaggaag
    25141 tcgcccaacg ccagcagaga ttgaagaaat ggaagagcat ttaaatgctt gcgatgatat
    25201 acttggctat catgatttat tggtgcataa ctatggaccc aataaacgct ttgcatcagt
    25261 ccacatcgaa gtagatgaat caatgaattt gaatgtggct cataagatta ttgatctgat
    25321 agaaaaagac tttaaaaagt ctttggatgt ggaacttgtc tgtcacttgg atccaatgcc
    25381 tgttgataac gaaaaatact ataatatctt gcggcagtta aagcaaattc ttcttgaaac
    25441 aggtacaggg cttaatattc atgattttag agtaattcat caaggaaaaa cgctgcaatt
    25501 tgatgtagtg gtccctgaga aattccagtt tgttgataca gaactagaaa gaatcttacg
    25561 tagtcaaata gaagaaaaaa tcggagatta ccaattggaa attacgtttg atcacaatta
    25621 tcttttatat taaatgagaa actataaaaa gactccttaa agtttttaat ttaaagagtc
    25681 tttttttctt ttaattgttt atggagagcc tgacgtgcaa gattatctgc tcctttattt
    25741 ttgttttcgg gcaaccattg caggatcaat aaagaaaatt cggatagaag ttcttggatt
    25801 ctttgaaggt agggaaggaa gagctcgttc ttcgttgcgt ttttgtcgac cgtagaaact
    25861 agtgttttgc tgtctgaaaa acagaaaatc gtttcattct gtaagtcgtt tttctttaga
    25921 aattctaaag tttttaaaaa gacttcgaac tctgcttgat ggtttgtccc tatagaaagc
    25981 gggatagata gctgttcttg tttaccttga tagacaatca agattccacc tccactagga
    26041 ccagggttgc cttttgtaga ggcatcaatg tatactttta acataaaaag agattctcct
    26101 ttttgttcaa actaatgtta tggtaagatt atatcattac tgaatcggtg agaggttaga
    26161 gaatgaaaaa aaatattcga tggcagcctg aactggcaca tacgatcatc tattggtcat
    26221 tgaccttgat cattctgttt tttagtctga ttctttcttt agaaaacaca ggtccttatt
    26281 ggaaaagcaa ttttgtttta ggtatttttc ttgtttttct atttctaggt ttgcggcgtt
    26341 cttttatttt aaaatcaaac tctgtgatta tacgttatgc tgctttttgg aaagaccgag
    26401 aaatatctgt cggacagatt cgatctttga cgccaactaa tactggggtg caaatgatgt
    26461 tgcaagatga gaaagcccca tctgttttca tgatgcgaaa aaaagatcaa gtagtccttt
    26521 ttgatcattt tgaaaaaaac ggacccaaat cagaatcaat tccttatcct tatacgaaaa
    26581 aaagaaaaag ctaattttaa aaatcagaac ctaaaaaaac gaatttttac aggaaaagaa
    26641 aagagtgaga cgtacggttc atcccgttta gcgtctcact ctatttgttt atctattcat
    26701 gcatactagt atcggtagtc tgaacgttgc ctttcttgac atgggcagct tgtgttcctc
    26761 gattcccttc gatgatctca aattcgacca cttgattttc ttccaaagtt ttgaatccct
    26821 cttcttcaat cgcagtaaaa tgtacaaaaa tttcttctgt gtcatcataa ctgataaagc
    26881 cgtatccttt tttgttgtca aaccatttta caattcctgt ggtcaaagca ctcacgacct
    26941 ttctaaagtc tgatcatagc cttcattata aagagtattt ataagtacgt caaataaatt
    27001 aaaaacctgt aattttatgt tttttttctg aataaaaaaa ggtataattg gcatatctta
    27061 acaaagaaga tattttgttg aataaagttc gtgaaaaggg gtaatcaaat gaagaatgat
    27121 ctgcaaattt cacaagaaac agagctagaa ccgattgttg aagtggctag taaattagat
    27181 cttaaggaag acgatttgga actgtatggg aaatataagg cgaaaatcaa tttttctgga
    27241 atcaatcgat caaaggataa cgctgagggg catttgatct tagttacttc catcaatcca
    27301 acaccagctg gcgaaggaaa gtcaacgatc actgtagggt taggagacgc gctcaataaa
    27361 atcaataaaa aatctgttat tgcattgcga gaaccctcac ttggtcctgt gatgggaatc
    27421 aaaggtggtg ctgcaggcgg aggatatgca caggtattgc ctatggaaga tatcaacttg
    27481 cattttactg gtgacatgca tgctatcaca acagctaata atgcattatc tgctctttta
    27541 gataatcata ttcatcaagg aaatgaattg aacatcgatg cccgccgagt gatttggaaa
    27601 cgggtagtcg atctcaatga tcgtgaactg cgtaaagtaa ttgttggctt gggtgggcca
    27661 atccaaggtg ttccaagaga agatgggttt gatatcacag ttgccagcga aatcatggct
    27721 atcttgtgtc ttgcggcaga ccttgaagat ttaaaggctc gtcttgcaag gatcgttgtc
    27781 ggctatacat ttgaccgtca gcctgtgact gctggagact tgaaagcaga aggcgctctg
    27841 gcattactgc tgaaagatgc aatcaagccg aatcttgtac aaacaattta cggaacacca
    27901 gcttttgtcc acgggggacc gtttgccaat atcgctcatg gctgcaatag tattttagct
    27961 accaagacag cattgagatt agcagattac gttgtcacag aggcaggatt tggtgcagat
    28021 cttggcggag agaaattttt agatatcaaa gtacctaact tgaaaaaagc gccggatgct
    28081 gtggtgattg tcgccactgt ccgtgcattg aaaatgcatg gcggaatgaa aaaagatgaa
    28141 cttaaaaatg agaatcttga tgctttgaaa gtaggttttg caaatttaaa gcgccatata
    28201 cgtaatatgg aacaatatca gctacctgtg atcgtcgcaa tcaatgaatt cgtaacagat
    28261 acagacaatg aactcacact gttggagcac ttgtgtgaaa atcaggggat tcttgccaag
    28321 agagcgagtg tatgggcaga tggagcagaa ggcggcgttg atttagcaaa agctgtagta
    28381 cgattgattg atagaaaaga agctaattac aagccattat accgcctaga agaaacgata
    28441 caagaaaaga cagaaacaat cgtcaagaaa atttacggag gaaatggtgt tgttttcagc
    28501 aaaaaagcgc aaaaacaaat agaagagttt acgaagaatg gatgggacaa actgccgatt
    28561 tgtatggcaa aaacacagta ttcattttct gatgatcctt cactgctagg tgcaccggaa
    28621 gatttcacca tcacgatccg cgaaatcatc ccaaaattag gggcaggatt tttagtagcc
    28681 ttgactggag atgttatgac gatgcctggt ttgcctaaaa aacctgctgc attgaatatg
    28741 gacgtgacaa atgacggaga agtgttggga ttgttttaag cttgattata atctaccatc
    28801 gaaggagctg tattttgact acagcttctt tttttagacg atagggaatt aattttgttt
    28861 acactaggat cattcttttt ctttattgta ttttgagcta tactaaagca taataggagg
    28921 gtatgatgaa aacgacattt ataagaaaaa ccggttttta cggtatgggt agctcgattc
    28981 agctaagaat caacaatgaa aaacgcagtg ttctcagtca tgaacaaaag ataacgcttg
    29041 atctggaagt gccctttacg atgcaggtca ccttttattg gctaaaaagc cccgtttata
    29101 cagtgaaaga agcaaagcct gcttatgtga tcacaatgaa ttacttgatt cttcaaatct
    29161 atccgttttt atttctagca actgggttgg gggccgtttt cctacaaaat ttatttttca
    29221 gtttttttgc agttattgtg ttgattgcgt tctttttcta tataaaaaac cgggcatatt
    29281 tgatcaagga ggaaaattat ggcaaactct gatctttttt taatcagttt caatcgaatc
    29341 gaaaaatgga tgagagacga gatgggaaat gcacgaaata tgggatttac cgaacttgtc
    29401 agacgcctag ctcaaagaaa acaattaatg atcagaaaat acgaagatga tcttttacag
    29461 cttgctcagt taaggaatgc gatcgtccat gatcggatcg cagtcgattt tgtcattgca
    29521 gagccaaatg attggacagt caagcgaatc cagctgatcg aacaagaact gattcagcct
    29581 gaaaccgtgc tgcctcgatt tgcaaaacgt gtaacaggtt ttgaacaaga tcttccgtta
    29641 cttgaactgt taaagatagt agcagaaaaa aggtattcac aatttccgtt atacaataaa
    29701 ggaaaatttg tagcattgat tactttacgt aatatcgggt tttggctagc aaaagaaagc
    29761 caaaaaggtc cggttgattt aacaaataaa cgagcgttgg atttgatcat ccaaaacgga
    29821 aagtacacaa attatcattt tgttccagct aaaacacata tttacgaggt tgaagcgatg
    29881 tttcgtgagc aagggacttt ggaagccgta cttatcacta aagatgggaa tcccgatggt
    29941 aatctgctcg ggatcgtacg acctcgtgat atttataaac aaatagaaaa ggattgattt
    30001 tttgttaatt atttattgga tattcagcgc tttgctagta ggagtggatc agttggtcaa
    30061 atggtggatc actgataatc tagcgcttgg agagacgaaa aatcttatac caggtatttt
    30121 gtcactgaat catattcgaa acactggagc cgcatggagt atgctagaag ggaagatgtg
    30181 gttctttact atagtgacac tcattgcagt cgttgtgatt ctcacattga tgattaaaaa
    30241 tcgtgaaaaa ggtaatcgct ggttcatgat tggattgagt ttgattctag ctggtgcgat
    30301 tggcaatttt atcgaccgta ttcgtcttgg ctatgtagta gatatgttcc agacagattt
    30361 tatgagcttt ccgattttta acgtagcaga tgtgacactt gtatgtggag tgatttgtat
    30421 gttaatttat attattttag atgaaaagga tcaaagaaaa aaatgacaga acttaaaacg
    30481 acgattcacc atgaatctgg taggatcgat aaagtattga ctaatttatt caccgattac
    30541 agtcgttcgc aaattcagct gtggctgaaa aatggagcgg tttctgtcaa tggacagcca
    30601 gtcaaagcaa attacaaagt aaaacaagga gacgagatta ctgtcttgat ccccgaacca
    30661 gaaacattag atgtggtccc agaaaatatc ccgttgacga tcgtttacga agatgaagat
    30721 gtagcggtga taaacaaacc tcaaggaatg gtcgttcatc cttcagcagg tcatacgcat
    30781 ggcacaatgg tcaatgcgct gatgtaccat atcaaggatc tttctacgat caacggcgtt
    30841 attcgtccag gtattgttca ccgaatcgac aaagatactt ccggactgct aatggttgcc
    30901 aaaaatgacc aggcacacga atcattagct cagcaattaa aagataagac ctctttgcgc
    30961 aaatatgttg ctttagtcca tggagagatt cctcacgaaa aaggaaccat caatgcgcca
    31021 atcggccgtt cgaaggaaaa ccggaaaatg caggcgataa gagaagatgg gaagtcggct
    31081 gtcacacatt ttactgtatt ggaacgtttc gaaggattta ctctggttga attaaaacta
    31141 gaaaccggaa gaacacacca gatccgtgtg catatgaagt atatcggtta tccgttagct
    31201 ggagatccag tatacggacc gaagaaaact ttgaaaggca atggtcagtt tctccatgcg
    31261 caaatgttag gttttacgca cccgactaca aacgaaaaga tgatctttga agcacctctt
    31321 ccagaagttt ttgaaaaaac tttagaaaaa ttaagaaaaa acattgcatt ttaatttaga
    31381 taacggtata gtaattaaca acgaaagcat aaaacttaat agaattcctt taagtttagt
    31441 cccgtgaggc taagaagggt gggagcaagg atacgtatgg tgcgccataa gtagattgct
    31501 cattgacata gtatagctat cagtgtatca gtatacgctc agtctaaaca ttcctcctgt
    31561 cctcttaggg caggagtttt tttatgcaaa aaagtagggg gaatgaaaat gcaagcaaaa
    31621 gaagtagtag atcaggtaac catgaagcga gcattgactc gaattactta tgagattatc
    31681 gagagaaacc atagtatcca agatatcgta ttggtaggta tcaaaacacg aggaatctac
    31741 attgcatcaa gaattgccga acgcctgaag cagctagaag atatcgatat tcctgtagga
    31801 gaactggata tcactttata ccgagatgac aaaaaagaaa atccagaaga accggaactt
    31861 cattcttctg acatcccagt ttccttagaa ggaaaagaag ttattttgat tgatgatgtt
    31921 ttgtatacag gacgaacgat tcgagcagca atggacgcag taatggattt tggcagacca
    31981 agaaaaattt cattagcagt cctggtcgat cgtgggcatc gagaattgcc aatccgtgca
    32041 gattatgttg ggaagaacat tccaacatct cgggcggaag agattttagt agagatgcaa
    32101 gagcttgatg gacaagatcg catcatgatt ttgaaagagg aagattagag gagagagaaa
    32161 catgtcggag aaaaaaggat ttcataatga tgacgtggtg ttagatatcc acgatcgccc
    32221 aaccccagga cattgggtag gattaagttt acaacacttg ttcacgatgt tcggagcaac
    32281 cgtgttagtc cccatcttag taggtatcga tccaggtatt gcgctagtca gttcaggact
    32341 aggtacattg gtgtacttat ttactactaa aggaaagata cctgcgtact taggaagcag
    32401 cttcgctttc attgcagcga tgcagctttt gatgaaaaca gatggttatc cagcgattgc
    32461 acaaggagca gtcacaactg gattagtcta tttgattgtt gcgctgatca tcaaaaagat
    32521 cggttcagca tggctggata aaatcttgcc tccaatcgtc gtcggtccag tcatcatggt
    32581 tatcgggttg ggacttgcgg caaatgcagc aacaaatgct atgtataatc agggcacgta
    32641 tgatttcaag tatattgctg tagcgctgat cacattagga ttaacaattt tcttcaacat
    32701 gtggctaaga ggttttctcg gattgattcc gattttacta ggtattatta gcggatacat
    32761 cgtagccttg ctgtttggaa tcgtggatac taagccaatc gtagacgctg cctggtttgc
    32821 tgttcctaac tttgagattc cctttgtaca atatgaacca aagctatatc ttggagcaat
    32881 cacaacaatg gcacccatcg cttttgtgac gatgacagag catatcggcc atcttatggt
    32941 attgaataaa ctaactaaac gtaatttctt tgaagaaccg ggtcttcata agacgttgac
    33001 aggtgatgga ttagcacaaa tcgtggcagc ctttgtcgga ggtcctcctg taacaagtta
    33061 cggtgaaaac attggtgttc tggctatcac acgtgtccat agcgtgtttg tgatcggtgg
    33121 agctgcagta tttgcagtca ttttaggatt cgtaggaaaa ttaagcgctt tgattttaag
    33181 tatccctgga ccagtcatct caggaatcag ttttatctta tttggagtaa ttgccgcaag
    33241 tggaatgaag attttaatcg agaacaaaat cgattttgat aaaaagaaaa atctgttgat
    33301 tgcttcagtt atcttagtag taggaattgg cggactcgtc tttgaagtag gcacatttac
    33361 attgtcggca atggcattag caactgtact tggcatcatt ttaaatctta tcttaccaga
    33421 aacttcacga agcgaagaaa actaaggagg aaacattatg atcatcaaat cagaacgtat
    33481 cagcttaaag catttattga ccgttgaggc acttactgat caagaagtta tgggcttgat
    33541 ccgcagaggg caggaattca agcaaggtgc taattggact ccgcaaaagc gccagtactt
    33601 tgctacaaac ctctttttcg aaaatagtac tcgcacacat aaaagcttcg atgtagcgga
    33661 gaaaaaacta gggctagaag tgattgaatt tgaagcaagc acaagctctg tccaaaaagg
    33721 ggaaacacta tacgatacag tactgacgat gtcagcatta ggcgtagacg tagcggttat
    33781 ccgtcatggt gatgagaact attatgacga actgatacaa agtaaaacaa tccagtgttc
    33841 gattatcaat ggaggagacg gaagcggaca gcatccaacc caatgcttgc ttgatttgat
    33901 gaccatttat gaagaatttg gtcattttga aggtctgaaa gttgccatcg taggtgatat
    33961 cactcactct cgcgtagcta aatccaatat gcagatgtta aaacgtctag gcgcacaagt
    34021 attcttctca ggtcctagag aatggtacga cgatgaattc gaagtgtacg gacattatat
    34081 gccattggat gaactactag atcaagttga tgtcatgatg atgcttcggg tgcaacacga
    34141 acgccatgat ggaaaagaaa gtttctcaaa agaaggctac catcaggaac atgggttaac
    34201 agttgagcgt gcgaagaagt tgcaaaaaca tgcaatcatt atgcacccag caccagttaa
    34261 ccgtgatgta gaattagctg attcattagt ggaaggatta cagtcgcgga tcgttcagca
    34321 aatgagcaat ggagtattcg tcagaatggc aatcttagaa gcagtgctaa atggaaaagc
    34381 gtaattatag gaggatgaaa aaatgaaaac actcattaaa aacggtcaga tcaatacacg
    34441 aaaaaatatg acgacaccag cagaaatttg gatagaagat gggcgaatca aagcaatcgg
    34501 tacaggattc tcagaagccg aatttgatga agtgtttgat gcaaagggac aattgatcac
    34561 acctggttta gtggacgtcc atgttcattt gagagagcca ggatttacat acaaggagac
    34621 gatcgaagct gggactcgct cagctgctcg tggcggtttc actactgtat gcgcgatgcc
    34681 gaatttaaat cctgtacctg atacggcaga aaagctgaga caagtatatg acatcattag
    34741 aaaagatgca gttgtgaagg tattgcagta tgcgccaatc acagaaaatc tgcgaagtga
    34801 gaaattagtt gatcaagaag cactgattga agaaggtgct tttgctttca caaatgacgg
    34861 tgtaggtgtc caaacagctg ggacgatgta tctggctatg aaagaagctg caaaaaacaa
    34921 taaagcccta gtagcccata cagaagatga atctttatta tttggcggcg ttatgcacgc
    34981 tggcaagaag gcagaagaac ttggtcttcc cgggatctta agcgtaaccg agtcttctca
    35041 aattgctaga gatctgttgc tagctgaagc aactggtgtt cattaccatg tttgccatgt
    35101 ttctactaaa gaaagtgtcc gtgtaatcag agacgcaaaa aaagcaggaa tccacgtaac
    35161 agcagaagtt tcaccccatc atcttatttt gatcgacgaa gatattcccg aagatttcgg
    35221 cttttggaaa atgaacccgc cgctaagagg acgggaggac agagaagctt tgatcgaagg
    35281 cttgcttgac ggtacgatcg attgcatcgc tacagaccat gccccccatg gattagaaga
    35341 aaaaagccaa agtttcatga aatcaccatt tggtatcgta ggaagcgaaa cagcgtttca
    35401 actgatctat actcattttg tagaaactgg tcgattcacg ttggaacaag tgatcaattg
    35461 gttggcagtt aaaccggctg agatttttgg tttgaatgca ggaacgctaa cggttggtgc
    35521 ccccgctgat gtagcagttt ttgatattac acaaacttgc acgatcgata aagaagactt
    35581 tttatcaaaa ggggagaata caccttttat cggctggaaa gtaaaaggcg aaacacaaat
    35641 gacttttgtc aacggaaaac ttgtgtggca gaagggagaa taaaagtatg gaacgtttgc
    35701 ttatattaga agatggaaca gttttcgaag gaaaggcttt tggtgcagat gctagtgtct
    35761 tcggagaaat cgtttttaca acaagtatga ctggttatca agaaactatc actgatcaaa
    35821 gttttaacgg tcaaatcatt acatttacct atcctatggt gggtaattat ggggtaaata
    35881 gagatgatta tgaatcgatt tctccaacat gcaaaggagt agtagtaaaa gaacatgcac
    35941 gtgtagcttc aaactggcga aaccaaatga cgctagattc atttttgaaa caaaaaggaa
    36001 ttcctggaat atcaggtatc gatacgcgcg cccttactcg caaaatccgt gaagtcggaa
    36061 cgatgaaagc tagtatcgtt gatgctggag attccttcga acatgcattt gatcagctga
    36121 aagcaaccgt tctagcaacc aatcaagtgc agcaagtctc aactaacaaa ccttatccaa
    36181 gtccaggaac aggtcggaaa gtcattgtca ttgacttcgg cttgaaacat agtattttac
    36241 gagaattgag taaaagaaac tgtaacctca cagtacttcc ctataacaca gatgccgaga
    36301 cgattttagc gctatcacca gacggagtca tgctgacgaa tggacctggt gatccaaaag
    36361 atgtgccaga agcaatcgaa atgatcaaaa aaatccaagg aaaagtaccg atttttggta
    36421 tttgtctcgg tcaccagtta tttgctttag ccaacggtgc agatacctat aaaatgaaat
    36481 tcggacatcg aggattaaat cacccggtac gagaagtagc gaccggaaga attgatttta
    36541 cttctcaaaa tcatggctat gcagtcgatg aagccacgat tgatccagaa aaactcatcg
    36601 taactcatgt agaagtaaat gacggaacag tagaaggagt caaacacaga gattatcccg
    36661 ctttcagtgt gcagtttcat ccagatgctg cccctggtcc gcatgatgca ctacatttat
    36721 ttgatgaatt tatggaaatg atggacgcag gaaaggagca gtaaacagat gccgaaacgt
    36781 acagatatca agaaaattat ggtgattgga tctggtccga tcatcattgg tcaagccgca
    36841 gaatttgact acgcaggaac acaagcctgc cttgccttaa aagaagaagg atatgaagtg
    36901 gttttagtaa attctaatcc tgcgacgatc atgacggata aagaagtggc agatcaagta
    36961 tatatcgaac cgatcacact tgaatttgta tcaaggattc ttcgaaaaga gcgtccagat
    37021 gctctccttc caactttagg cggacagaca ggcctcaata tggcaatgga gctggcagca
    37081 tcaggtattt tagaagaatt gaacatagaa cttctaggaa caaagctttc tgcgatcgat
    37141 caagcagaag atcgtgatct attcaaacag ttgatgaaag aattgaatca gccaatcccc
    37201 gagtctgaaa tcgtcaatac agtagaagaa gctgtagatt ttgcaaataa aatcggctat
    37261 cctgtcatcg ttcgtccagc ctttacttta ggcggaactg gtggaggtat gtgtgaaaat
    37321 gaagaagaat tacgtatcgt atcagaaaat ggattaagtc tttcccccgc tacccaatgt
    37381 ttgattgaac gaagtattgc aggttttaaa gaaattgaat atgaagtgat gagagattcg
    37441 gctgataatg caatcgttgt ctgcaatatg gaaaattttg acccggtggg tatccataca
    37501 ggtgattcta tcgtatttgc ccctagtcaa acattgtctg accatgaata tcaaatgctt
    37561 cgtgatgctt ctctttcgat tattcgagca cttaagatcg agggaggctg taatgtccaa
    37621 ctcgctcttg atccacatag ttttaattat tatgtgattg aagtcaatcc gcgtgtttct
    37681 cgttcatctg cgttagcaag taaggcgact ggctatccga tcgctaagct agctgctaaa
    37741 atagcgcttg gattaacatt agacgaaatg aaaaatcctg ttacaggaac gacttttgca
    37801 gaatttgaac ctgcattaga ttatgttgta gctaaaattc ctcgctggcc gtttgataag
    37861 tttgaaaacg gagaacggct tttaggcacg cagatgaaag caactggtga agtgatggct
    37921 atcggtcgga atatcgaaga gtctttattg aaagctgtgc gttctttaga aatcggtaca
    37981 caccatatcg aacttcctga gttggcagac atcacggacg atgacctaat ccaaaaagtg
    38041 gttcgagcac aagatgatcg cctgttttat gtagctgaag ctattagaag agggtacacc
    38101 atagaagaat tagcggattt gacaaaaatc gatttattct ttttagataa actgttgcat
    38161 atcgtagaaa tcgaacagca gttgaaacag cagccgttgg atgacaagat attgaaaaca
    38221 gcgaaacaaa acggttttac agacaaaaaa gtggcggagc tgtgggaaat aaacgaagaa
    38281 gccgttcgcc agatgagaaa agaaaatgat ttgattcctg tatataagat ggttgacact
    38341 tgtgcagcgg aattcgaatc aaaaacacct tatttttata gttcctatga atatgaaaat
    38401 gaaagttcac gatcagaaaa gccctcaatt ctagtactgg gatctggtcc tatccgtatc
    38461 ggacaagggg ttgaatttga ctatgcgact gtgcattctg tcaaagcaat ccagcaggca
    38521 ggatacgaag caatcatcat gaacagcaat cctgaaacag tttcgactga cttttctatt
    38581 tcggataaat tatattttga gccattgacc ttcgaagatg tgatgaatgt gatcgagcta
    38641 gaacagccga taggagtaat cgttcaattt ggtggtcaga cagcaatcaa cttagccgaa
    38701 cctttgactg ctgctggtgt aaaaatctta gggacaacga tcgaagactt ggatcgtgcg
    38761 gaaaatcgtg atttattcga gcaagcgctg aaagagttgg atattcctca acctttaggg
    38821 gatactgcta caagtaaaga agaagccgtg gttatcgctt cgagaattgg ttatccagtt
    38881 cttgttcgtc caagctatgt attaggcgga cgtgcgatgg aaatcgttga aaaccagcga
    38941 gatttagaag attacatgga acatgctgtg aaagcatctc cagaacatcc agtactagtg
    39001 gaccgttact tgcttggtgg tgaatgcgaa gtggatgcga tttgtgacgg tgaaacagta
    39061 ctcatcccag ggatcatgga gcatattgag cgtgctggtg tccattcagg ggattcaatg
    39121 gctgtgtatc cacctcaaac attatctgaa tcgatcaaga aaacgatcga agattacacg
    39181 atccgcttag cattaggttt gaattgtgtc gggatgatga atatccagtt tgtcatccat
    39241 gaaaatgagg tttatgtgat cgaagtcaat cctcgagcaa gtcggactgt tccattctta
    39301 agtaaggtga caggaattcc aatggcacaa attgcgacga aagctatttt gggagagaaa
    39361 ctaaagacac ttggttatga aaacggactt tatccagaaa caaaagaagt tcatgttaaa
    39421 gcacctgtct tttcttttac gaaattgcag aaagttgata cttacttagg gcctgaaatg
    39481 aaatcgactg gagaagtaat gggatctgac agacaattag acaaagcttt gtacaaggca
    39541 ttcgaagctt ctggattaca cttgcctgaa tatggagccg tattatttac gattgcggac
    39601 gaaacaaaag aagaagcact agaattagct aaacgtttta cagaaattgg ctatagtctg
    39661 ataggaacga aacatacagc tgcttatttc gaaaaaaatg gtctggtcgt tgaaacagta
    39721 gcgaaaattt ctgaggaagc agcagaaaaa aatgtagtcg acttaataag agatggtaaa
    39781 acacaagtgg ttgtcaatac gattgataaa gatcggggaa atgcttctaa agacggtttc
    39841 atcatccgaa gagaagcagt tgaacatggt attccattgt ttacttcttt agacacagca
    39901 gatgcgatcg ttcgagtgat ggaatcccgt gctttttcaa ctaaccctat ttaacggcga
    39961 gcacatgtta agacaaaaag acgaaaaacc gttgcaggag gggacagatg aaacaagaaa
    40021 tgatggaaat cgttcagcaa aaacagctgg caccaaagat ttttgaaatg acattgaaag
    40081 gtgatctagt aaaagaaatg cagcaaccag gacagttctt gcatattctc gtgccgcgtg
    40141 cagacttgct attacgccga ccgattagct tgaatcatat cgaccacgaa acaaatactt
    40201 gccgtatcat ttatcgggtg gaaggtgagg ggactagagt cttctctact ctttctgccg
    40261 gagaccattt agatgtcatg ggtcctttag gcaatggttt tgatttgtcc aatcttcata
    40321 caggagacga agtatttata atcggtggag gaatcggtgt ccctcctcta tatgagttat
    40381 caaaacagtt agtcgaaaaa ggaattaagc caacgcattt tttaggtttt gccacacatg
    40441 aagtcgttta ttatgaaaaa gaatttaatc gtctaggaga aacaagaatc gctactgacg
    40501 atggaacata cggtatgcac ggaaatgtcg gtaatttatt gttaggaacg aagacggaac
    40561 ctacagctgt ttttgcatgt ggttcaaacg ggttattgaa gacagtggaa cagctgtttt
    40621 cttcgcatca aaatgtccag ttatcattag aatcccgcat ggcatgtggg ataggggctt
    40681 gctatgcttg cgtttgtcat acgccggaag atgaaacagg aacaaaaagt gtgaaagttt
    40741 gtgacgaagg accgattttt aaaataggag aggtaattat atgaatgaga atcctttagc
    40801 ggtcaaactt cctggcctag aattaaaaaa tcctatcatg ccggcaagcg gctgtttcgg
    40861 ttttggcaaa gaatacggaa aatactatga tttgaatcag cttggatcga tcatggtcaa
    40921 ggcaacgact ccaaatgccc gctttggcaa tcccacgcca agagtggcgg aaacaccatc
    40981 tggcatgttg aatgcaattg gtttgcaaaa tcctggtcta gacgttgtta tgcataaact
    41041 tcttccagaa ttagaggagt acaatgagtt acccatcatc gcaaatgttg ctggtgcttg
    41101 tgaagaagac tatgtagaag tttgtagcaa aattggcgaa gcaccaaatg tgaaagcaat
    41161 cgagctgaac atttcttgcc caaatgtcaa acatggcgga attgcttttg gaacagattc
    41221 tgatgttgct tttcaattaa cacaagcagt aaagaaagtt tcttctgttc ctgtctatgt
    41281 caaattgtcc ccaaatgtaa cagacatcgt tccaatcgct caggcaatcg aagctggagg
    41341 ggcagatggg tttacgatga tcaatactct tcttggtatg cggattgacc ttaagacaag
    41401 aaaaccgatt ttagccaatc aaacaggtgg actctccgga ccagcaatca agcctgtcgc
    41461 tattcgtttg attcaccaag tagcaagtat atccagcctt ccaatcattg ggatgggcgg
    41521 cgtgcagaca gtggatgatg ttcttgagat gtttatggct ggagcaagtg ctgtagctat
    41581 agggaccgcg aatttcaccg atccgtatat ctgtcctaaa ctaattaaag agctccctgt
    41641 gcgaatgagc gaactaggca tcgaatcgct ggaaagatta atcaaagaag tgagagagga
    41701 acgtgaacga tgaatcaact agaccaacga ccaattattg cattagattt ttcaactagg
    41761 caagaggtag aagactttct tcgatttttc ccgaaagaag aaaaattgtt tgtgaaaatc
    41821 ggaatggaat tgttctacca agaaggacct gaaatcgtac gttatctaaa agaggctggt
    41881 cataatgtat ttttagatct gaaacttcac gatattccga atacggtcga aaaagcaatg
    41941 cggggattgg caaagcttgg tgttgatctc acctgtgttc atgccgctgg cggtattcgt
    42001 atgatggagg cggcaatgcg aggattagaa gaggggactc cagaaggtgg aaaacgtccg
    42061 ctacttctag ctatcactca gcttacttct acaagtgaag aagaaatgca tgccgatcaa
    42121 ctaatcgaag tttctttaga aaaaagtgtg atccattatg ctagctgtgc caaaaaagca
    42181 ggattagatg gtgtggtttc ttctgcttgg gaagtagaag cgatcaaaga aatggcagga
    42241 gacgaatttg tttgcttaac tccgggtata agaccagaag gaacagtcgc aggagatcag
    42301 acaagagtag tcacaccttc tcaggcaaaa aagatcggct caacttttat cgttataggt
    42361 cgaccaatca ctcagtcaac taatccttat aaagcatatc aaacgattca aacagaatgg
    42421 agctaaccta aaatgaatgt tgaacaaagt atcgcaaaag acttattaga aattgaagca
    42481 gtatttttaa atccaagtaa tccttttaca tgggcaagtg gaatcaaaag cccaatttac
    42541 tgtgataacc gaatcacgat gagctatcca aaagtccgaa aagagatcgc aaaaggatta
    42601 gcaagcaaaa tcaaagaagc ttaccctgaa gtacaagtga ttgctggaac tgcaacagca
    42661 ggaatccctc atgccgcatg ggtagcggaa atactggatt tgccaatggt ctatattcga
    42721 agtaaggcga aagatcatgg gaaaggtaac caaatcgaag gacgtatatt tgaaggtcag
    42781 aagatggttg tgatcgagga tttgatttct acaggcggaa gtgtcttgga agcagctgaa
    42841 gcagcaaaaa gagaaggtgc ggatatcctt ggtgttgcgg ctatctttac gtatgagttg
    42901 ccaaaaggaa aagcaaattt cgaaaaagcc gaaatccctc ttatgacgtt gaccaattat
    42961 tctgtattga ttgaagcagc tttagaagat cgttatatcg atgagcaaga attaactctg
    43021 ctaaaagaat ggaaaaaaga tcctgaaaat tggcagattt aagcgcgctt gatgaagata
    43081 gagaaatccg atttgtgtct ccagaatcat caattggaat agcaaaaatc caaaaactga
    43141 ttgcgagaag catcttttca tcaggtatga aaagaaactt ctatcgtgat cagtttttga
    43201 tttttttaaa attcattttt ctaagactgg ttagcagaaa gaagagaata atctcttctc
    43261 ttttttctat tatatataga ttttctctat tttttggtaa gggagctgtt tcaaaaatgg
    43321 taaagagcgg aaatgttgcg aatatcagtt atttaatgat atgatattcg gaaaaagaaa
    43381 tctcaaaggg aggtaacttt tatggggttg aatgaattta ttttaggatt gaacaattta
    43441 gagacgatca caagggcacc tggatttttt aaatatacag agcatacagt tgcagcacat
    43501 tcgtatcgtg tagcatctat tgcgcaagtt ctaggagaca ttgaagaagc gaatggacaa
    43561 ccgatcgatt ggaaactgct ctatgaaaaa tcattgaatc atgactacac agagcgtttt
    43621 attggtgata tcaaaacgcc ggtgaaatac gcaagcaaag agttaagagg gatgctgcaa
    43681 acagttgaag aaaagatgac ggacgaattc atcaaccaag agattccagc tgagttccaa
    43741 gaagtttatc gccgtcggtt gttcgaagga aaagatgaaa ccgtagaagg agaactatta
    43801 gccatttcag ataaggttga ccttctttat gaatcttttg atgaaatcat caaaaataac
    43861 ccggaaaaag tttataagga gatgttcatt gaggcagtga ctacgattaa aagctttgaa
    43921 catcggcctt ctgttcagta tttctttaaa gagatatttc cggaactatt gaaccaggaa
    43981 ttttatggaa aagaggcgtt tttagaaacc gtctcaacga ttgtaaagaa agaagaaaaa
    44041 taaaaatgat atgagatgca gggggaagta tagttgaaaa agaatatgga tgtcgaatgg
    44101 ggctattctg gagacaaagg tccggaaaat tgggcgagtt tatgtgattg gtttgcgaga
    44161 ggtgcggaat ttccattgca gtcgcctatt catttgacca agggggaaga aaccaaaata
    44221 gaaggagaca cgctttcctt tcattatcaa aagcaacggt ttactgacaa ggaattcaaa
    44281 aacacgattc atttagtacc ttttgaccaa ctcagttatg tagagttcaa gaaagaacgc
    44341 tattatttga cagatattca tttccacttg ccttctgaac atatcatcaa cggaaaacag
    44401 gaagatattg aatttcattt tgttcatatg aacagcaaaa aagagaatct agttgtgggt
    44461 gtcatgtacc agctaatggt acaggaaggt tggctgtata atcaagaaaa tggggaaagt
    44521 tggaacttag aaaaacatga gcattgggta aatccggatg tgttctttcc ggagcaaaaa
    44581 agccatttcc attatattgg ctcattgaca actccgccta ccagcgggcc aatccaatgg
    44641 tttgtaatgg atcatgtagg aaaactgaac caagagtttc ttctcccctt cgatggtcag
    44701 tatgctcgac cgaacaaccg accaatccag cccttgaatg gaagagaaat ttattatttc
    44761 gaggagtttg agcaatgaat attcagcaga tgaaatacgt ggtcgctgta gcaaacaatg
    44821 gtagtttcag agaagcggca aagaaaatgt ttgtctccca gccaagtctt tcagctggta
    44881 tcaaagaatt agaaaccgag ttagggatca cgctgtttac tcgaacgaat cgaggggcat
    44941 atttaacaga agaagggcaa gacttcttaa atcgtgccga gaaaatattg gtccagcttg
    45001 atacattaga aactcactat ctaacgagtg aaaagagaga caatttttcg atcgctgccc
    45061 agcattatga tttcttaggt cctttgaccg cacagctact cgaggaattc aaagacgaga
    45121 tcaaacaatt tcggatcatg gagacgacaa cagcgagagt gatcgaagaa gtcaaagagc
    45181 agcacagtga gatcggcatt atttatatga atgagcataa tcaagctggt atcagaagat
    45241 atttacaaca aggagagcta agtggccgca gcattggaaa attcaaaacg catatttttt
    45301 taaggcaagg acatccatta gctgaacacg cagagatcac caaagaagaa ttgttatctt
    45361 atcctcaagt tcgttttaca caggatggaa ataatttccc gtatttttat gaagacctta
    45421 tcgagatccc tgatcaagaa acagtgattt atacgagtga tcgaggtact ttgatgaata
    45481 tcgttcttga aactgatgct tacgcttcag gatctggtat tgtgattgga gggattcgcg
    45541 agcacttgcg gttaatccct ttggcagaag gcgaactaaa tgaattttat atcatttatt
    45601 cggcgaaacg tccgctcagt gaaatcgctc aaagatttat caaagagctt actcggttac
    45661 tggaccagca gagctaagaa aagcagatga gaacgctgtt tattgaacac cgttgatcaa
    45721 aaaagagggg ttgtgacaga attttgtcac aactcctctt tttgtagcaa acaatttata
    45781 actaattgct gttgtgggaa agtacgcaaa tatgattcta gaaacactca ttgattacct
    45841 gttttgtttc atagctgtaa ttttttcttc ttctggtgtc acgataatcg tcttttggtt
    45901 ttcataaata acataaccag gcttggcgcc atttggtttt cggatatgct ttacctgtac
    45961 aagatcaacc ggcacttggg cagagtatcg gtattttgaa aaataggcag ctaactcggc
    46021 agcttctgta attgtttcat ctgagggctt gtcactttta atgatgacgt gcgaaccggg
    46081 tatatttttt gcatgaagcc aatagtcggt ttttttagct gtcttcattg tcagctggtc
    46141 attttgaaga ttgtttttac caacaagaat gagcgttccg tcagaagaaa agtattgatc
    46201 aggctgagat ggtttcttac gtttttgttt ttttaagttc ttctttttca ggtatccttc
    46261 tgaagtcaat tcttctttaa ttgcttcgat atccattggg ccggcaattt ccagttgaga
    46321 taagacagat tcaagatatt ggatttcatc tttggcttct tggatttgtt cgccaatcag
    46381 cttaacagcg ttcttcagct tttggtatcg atggaaatat ttctgtgcat tttgatttgg
    46441 tgtcaatgcc ggatctagtg ctattttgat tggccgatct tcttcataat aattaggtaa
    46501 aacaacttct tttgcgccgc gtggtacttg tgtcagaaaa gtagtaagta gttcgccatt
    46561 tctacggtaa ttctcagcgt tttcggattc tttgagtgtt tgttcacgtt ttttcaattt
    46621 atttttattt cgttttagtt cattttcgat ttttcgaatc agttctccgc cttgttgttt
    46681 tgcccgatct ttctctgctt tttcgtgata atagctatct aataattgac tgagtgttgg
    46741 ataagctgtt acagcagatg cctgttcgga caatacctga taagcaatcg gtgtgaaaaa
    46801 atctttattt tccgtttcgt aaaaagtagg aataggctga tggctgatgg ctgagaaaaa
    46861 ttcgtcccat acaaccatct tttcattagg acgttccagt aatcttttgg tcagctcttg
    46921 agcgatatcg aaaccgattc cctgaaactg acgttggatt gcttttggat ctagttcagt
    46981 ttgggaaagc cgctgaaaga ttttttcttt ctcggagcta aatgggttca agacctcttg
    47041 ttttggcgga gcaacatatt ctacaccagg aagcaaagaa cgataagtgt tttgagagct
    47101 tccaatgtgt ttgatcgcat ccagtatttt tccagtttct cgattaacta agacgatcgt
    47161 actatgacgt cccattaatt caacgatcaa tatgatattt tgtagatcac ccaactcgtt
    47221 tcgtttagca aagtggaaat ggatcactcg atcattttcg atttgttcga tgtcttctaa
    47281 aatagcgcca tccaaatatt ttcgaagcat catcacgaag ttaggcggat tatcaggatt
    47341 ctgataatcg atttgggtaa tctgtacacg agcataactt gggtgcgcag ataataataa
    47401 tcgatggttt ttgccgcgtg aacgaatcac cagcacgatt tcattttcat aaggctgatg
    47461 gattttcgag atccggccag atagtaacgt ttcacgcaat tcgttgacca tcgcgtgggt
    47521 aaatacacca tcaaaggaca tggttcattc ctcatttctt ttcataagta tattcattat
    47581 aacgaaaaaa agaagaaaaa ccaatttggc cgaatagtaa agcaaaagtc tagaaaaatc
    47641 ttggactaat tacataaaaa acagtgtttc tatcgttgaa atccaaattt ttagg
//
LOCUS       DAZUMM010000010        47636 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000010 DAZUMM010000000
VERSION     DAZUMM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.010"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            38..226
                     /locus_tag="ACG8E6_000519"
     CDS             38..226
                     /locus_tag="ACG8E6_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFS5060966.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(284..637)
                     /locus_tag="ACG8E6_000520"
     CDS             complement(284..637)
                     /locus_tag="ACG8E6_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5060967.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1168..1590
                     /locus_tag="ACG8E6_000521"
     CDS             1168..1590
                     /locus_tag="ACG8E6_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5060968.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            1587..1817
                     /locus_tag="ACG8E6_000522"
     CDS             1587..1817
                     /locus_tag="ACG8E6_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5060969.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2315..2515
                     /locus_tag="ACG8E6_000523"
     CDS             2315..2515
                     /locus_tag="ACG8E6_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFS5060970.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            2542..3735
                     /locus_tag="ACG8E6_000524"
     CDS             2542..3735
                     /locus_tag="ACG8E6_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5060971.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(3803..5368)
                     /gene="guaA"
                     /locus_tag="ACG8E6_000525"
     CDS             complement(3803..5368)
                     /gene="guaA"
                     /locus_tag="ACG8E6_000525"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS5060972.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5427..6350)
                     /gene="coaA"
                     /locus_tag="ACG8E6_000526"
     CDS             complement(5427..6350)
                     /gene="coaA"
                     /locus_tag="ACG8E6_000526"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFS5060973.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            6546..7151
                     /locus_tag="ACG8E6_000527"
     CDS             6546..7151
                     /locus_tag="ACG8E6_000527"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5060974.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7459..8475
                     /locus_tag="ACG8E6_000528"
     CDS             7459..8475
                     /locus_tag="ACG8E6_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5060975.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            8616..9917
                     /locus_tag="ACG8E6_000529"
     CDS             8616..9917
                     /locus_tag="ACG8E6_000529"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFS5060976.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            9963..10625
                     /gene="deoC"
                     /locus_tag="ACG8E6_000530"
     CDS             9963..10625
                     /gene="deoC"
                     /locus_tag="ACG8E6_000530"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFS5060977.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            10646..11044
                     /locus_tag="ACG8E6_000531"
     CDS             10646..11044
                     /locus_tag="ACG8E6_000531"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFS5060978.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11110..12192
                     /locus_tag="ACG8E6_000532"
     CDS             11110..12192
                     /locus_tag="ACG8E6_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5060979.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12404..13966
                     /locus_tag="ACG8E6_000533"
     CDS             12404..13966
                     /locus_tag="ACG8E6_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5060980.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            13959..15092
                     /locus_tag="ACG8E6_000534"
     CDS             13959..15092
                     /locus_tag="ACG8E6_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5060981.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15092..16048
                     /locus_tag="ACG8E6_000535"
     CDS             15092..16048
                     /locus_tag="ACG8E6_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5060982.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16147..17313
                     /gene="deoB"
                     /locus_tag="ACG8E6_000536"
     CDS             16147..17313
                     /gene="deoB"
                     /locus_tag="ACG8E6_000536"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFS5060983.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17337..18155
                     /locus_tag="ACG8E6_000537"
     CDS             17337..18155
                     /locus_tag="ACG8E6_000537"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS5060984.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18172..18879
                     /gene="deoD"
                     /locus_tag="ACG8E6_000538"
     CDS             18172..18879
                     /gene="deoD"
                     /locus_tag="ACG8E6_000538"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS5060985.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19177..19863)
                     /gene="gpmA"
                     /locus_tag="ACG8E6_000539"
     CDS             complement(19177..19863)
                     /gene="gpmA"
                     /locus_tag="ACG8E6_000539"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS5060986.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20035..20616)
                     /locus_tag="ACG8E6_000540"
     CDS             complement(20035..20616)
                     /locus_tag="ACG8E6_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5060987.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(20749..21432)
                     /gene="rpiA"
                     /locus_tag="ACG8E6_000541"
     CDS             complement(20749..21432)
                     /gene="rpiA"
                     /locus_tag="ACG8E6_000541"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFS5060988.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            21751..22164
                     /gene="rpsL"
                     /locus_tag="ACG8E6_000542"
     CDS             21751..22164
                     /gene="rpsL"
                     /locus_tag="ACG8E6_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFS5060989.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22248..22718
                     /gene="rpsG"
                     /locus_tag="ACG8E6_000543"
     CDS             22248..22718
                     /gene="rpsG"
                     /locus_tag="ACG8E6_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFS5060990.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            22812..24899
                     /gene="fusA"
                     /locus_tag="ACG8E6_000544"
     CDS             22812..24899
                     /gene="fusA"
                     /locus_tag="ACG8E6_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFS5060991.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            25071..26258
                     /gene="tuf"
                     /locus_tag="ACG8E6_000545"
     CDS             25071..26258
                     /gene="tuf"
                     /locus_tag="ACG8E6_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS5060992.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            26665..26973
                     /gene="rpsJ"
                     /locus_tag="ACG8E6_000546"
     CDS             26665..26973
                     /gene="rpsJ"
                     /locus_tag="ACG8E6_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFS5060993.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            27004..27633
                     /gene="rplC"
                     /locus_tag="ACG8E6_000547"
     CDS             27004..27633
                     /gene="rplC"
                     /locus_tag="ACG8E6_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFS5060994.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            27661..28284
                     /gene="rplD"
                     /locus_tag="ACG8E6_000548"
     CDS             27661..28284
                     /gene="rplD"
                     /locus_tag="ACG8E6_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFS5060995.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            28284..28574
                     /gene="rplW"
                     /locus_tag="ACG8E6_000549"
     CDS             28284..28574
                     /gene="rplW"
                     /locus_tag="ACG8E6_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFS5060996.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            28613..29446
                     /gene="rplB"
                     /locus_tag="ACG8E6_000550"
     CDS             28613..29446
                     /gene="rplB"
                     /locus_tag="ACG8E6_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFS5060997.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            29489..29767
                     /gene="rpsS"
                     /locus_tag="ACG8E6_000551"
     CDS             29489..29767
                     /gene="rpsS"
                     /locus_tag="ACG8E6_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFS5060998.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            29790..30137
                     /gene="rplV"
                     /locus_tag="ACG8E6_000552"
     CDS             29790..30137
                     /gene="rplV"
                     /locus_tag="ACG8E6_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFS5060999.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            30151..30807
                     /gene="rpsC"
                     /locus_tag="ACG8E6_000553"
     CDS             30151..30807
                     /gene="rpsC"
                     /locus_tag="ACG8E6_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFS5061000.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            30810..31244
                     /gene="rplP"
                     /locus_tag="ACG8E6_000554"
     CDS             30810..31244
                     /gene="rplP"
                     /locus_tag="ACG8E6_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFS5061001.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            31234..31422
                     /gene="rpmC"
                     /locus_tag="ACG8E6_000555"
     CDS             31234..31422
                     /gene="rpmC"
                     /locus_tag="ACG8E6_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFS5061002.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            31447..31713
                     /gene="rpsQ"
                     /locus_tag="ACG8E6_000556"
     CDS             31447..31713
                     /gene="rpsQ"
                     /locus_tag="ACG8E6_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFS5061003.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            31771..32139
                     /gene="rplN"
                     /locus_tag="ACG8E6_000557"
     CDS             31771..32139
                     /gene="rplN"
                     /locus_tag="ACG8E6_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFS5061004.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            32176..32484
                     /gene="rplX"
                     /locus_tag="ACG8E6_000558"
     CDS             32176..32484
                     /gene="rplX"
                     /locus_tag="ACG8E6_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFS5061005.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            32512..33051
                     /gene="rplE"
                     /locus_tag="ACG8E6_000559"
     CDS             32512..33051
                     /gene="rplE"
                     /locus_tag="ACG8E6_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFS5061006.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            33070..33255
                     /locus_tag="ACG8E6_000560"
     CDS             33070..33255
                     /locus_tag="ACG8E6_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFS5061007.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            33291..33689
                     /gene="rpsH"
                     /locus_tag="ACG8E6_000561"
     CDS             33291..33689
                     /gene="rpsH"
                     /locus_tag="ACG8E6_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFS5061008.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            33721..34257
                     /gene="rplF"
                     /locus_tag="ACG8E6_000562"
     CDS             33721..34257
                     /gene="rplF"
                     /locus_tag="ACG8E6_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFS5061009.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            34418..34774
                     /gene="rplR"
                     /locus_tag="ACG8E6_000563"
     CDS             34418..34774
                     /gene="rplR"
                     /locus_tag="ACG8E6_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFS5061010.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            34795..35295
                     /gene="rpsE"
                     /locus_tag="ACG8E6_000564"
     CDS             34795..35295
                     /gene="rpsE"
                     /locus_tag="ACG8E6_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFS5061011.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            35310..35489
                     /gene="rpmD"
                     /locus_tag="ACG8E6_000565"
     CDS             35310..35489
                     /gene="rpmD"
                     /locus_tag="ACG8E6_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFS5061012.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            35533..35973
                     /gene="rplO"
                     /locus_tag="ACG8E6_000566"
     CDS             35533..35973
                     /gene="rplO"
                     /locus_tag="ACG8E6_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFS5061013.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            35973..37268
                     /gene="secY"
                     /locus_tag="ACG8E6_000567"
     CDS             35973..37268
                     /gene="secY"
                     /locus_tag="ACG8E6_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFS5061014.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            37330..37977
                     /locus_tag="ACG8E6_000568"
     CDS             37330..37977
                     /locus_tag="ACG8E6_000568"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFS5061015.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            38168..38386
                     /gene="infA"
                     /locus_tag="ACG8E6_000569"
     CDS             38168..38386
                     /gene="infA"
                     /locus_tag="ACG8E6_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFS5061016.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            38419..38535
                     /gene="rpmJ"
                     /locus_tag="ACG8E6_000570"
     CDS             38419..38535
                     /gene="rpmJ"
                     /locus_tag="ACG8E6_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFS5061017.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            38553..38918
                     /gene="rpsM"
                     /locus_tag="ACG8E6_000571"
     CDS             38553..38918
                     /gene="rpsM"
                     /locus_tag="ACG8E6_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFS5061018.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            38946..39335
                     /gene="rpsK"
                     /locus_tag="ACG8E6_000572"
     CDS             38946..39335
                     /gene="rpsK"
                     /locus_tag="ACG8E6_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFS5061019.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            39415..40353
                     /locus_tag="ACG8E6_000573"
     CDS             39415..40353
                     /locus_tag="ACG8E6_000573"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFS5061020.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            40381..40761
                     /gene="rplQ"
                     /locus_tag="ACG8E6_000574"
     CDS             40381..40761
                     /gene="rplQ"
                     /locus_tag="ACG8E6_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFS5061021.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            41033..41872
                     /locus_tag="ACG8E6_000575"
     CDS             41033..41872
                     /locus_tag="ACG8E6_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5061022.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            41848..42717
                     /locus_tag="ACG8E6_000576"
     CDS             41848..42717
                     /locus_tag="ACG8E6_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5061023.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            42707..43504
                     /locus_tag="ACG8E6_000577"
     CDS             42707..43504
                     /locus_tag="ACG8E6_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS5061024.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            43707..44453
                     /gene="truA"
                     /locus_tag="ACG8E6_000578"
     CDS             43707..44453
                     /gene="truA"
                     /locus_tag="ACG8E6_000578"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS5061025.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(44786..47455)
                     /locus_tag="ACG8E6_000579"
     CDS             complement(44786..47455)
                     /locus_tag="ACG8E6_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFS5061026.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT        15377 a         8761 c        10177 g        13321 t
ORIGIN      
        1 tatagatttg aattaaagtg gaaaggagga gattgttatt ataaactaca agtggatatt
       61 gtgtcctatt tgtggaaata aaacaagact acgaatacga gtggatacta tacttaaaaa
      121 tttcccttta tactgcccca aatgtaagaa cgaaacttta attaatgttc aaaaaatgaa
      181 tataataaca atcaaagagc cagacgccaa gacgcagagc cgataatttg agaaatgaaa
      241 ctctcatctt atcggctctt tttgtttatc tgaattttac tgactagcct tcaatatttc
      301 ttgcttcatt gatacctttt gctagtgctt ccattaagga tagttctttg tctgtaaagc
      361 tatccatgta tttttctacc tgtaaccttc gagtgctttt aatcaaatta tctgtaggta
      421 agaaaaattc atcaatagat acatggagta atgatacaag atcatacagc acttgtgtac
      481 ttgggtgttg tcctttgttt tctatatttg ttaaataacg tgggtcaatt tcaatcattg
      541 ttcccacttg ttcacgagtg agaccccgtt tcattcgagc ctctttaatc gctaaaccaa
      601 cggctctaaa atcgtattta tcttcttttt tacgcatact aaatcacctc tatacatttt
      661 actgttccta tataaccata aacaggtata gaaaaacatg ttatatagtt tatgagttcc
      721 tatagaacac tgtggaattt attaatgcga tgaatccaat ttcaaataga aaggtcgctg
      781 attttattta cctatacaac tttataaaat ttagtcatac tcctagaaag tagataaaat
      841 tggcgactta atggagggaa aagtcgccat gagaaagata atctgtcatc tagttctctt
      901 aattaagttc ttatcaaaaa aacttatcca cctgtactgg atagatatga ccatcaatgt
      961 ggttttgcag attacataga tagaaaaatc aaagcatatg aacagagaaa acaatctgtt
     1021 tatgtgcttt tttggtcact taataaatat cttttaggat ttctcttcat ttatgcaaca
     1081 aatcccccgt tcgtgtcggg gtttagtatg aagggagata aagaagcaca tgccactccc
     1141 tttcacgaca gaaagggggt gaaaagcatg aaaccatctt cttttcaaga aaccatagaa
     1201 aatcagtttg actatatctg caaacaagtt attgaagatg aacgaaaaga ttacttcaaa
     1261 catctagaca gactatcagc aaaagaagtc tcgttttctg atatagataa ctaccttgtc
     1321 aactcttttt ctactgttga tacgtacgtc actgatttcc aactatttaa attatttgga
     1381 ttaagaatca atgttgaaag tgatttatta agtgaagccc tgcgtcatct ttcagagaaa
     1441 aaacggaaca ttattttact tcattacttt atggatatga gtgatgtgga gattgcggag
     1501 ttactaaatt tgaatcgttc gactgtttat cgtcatcgaa taagtggact agccatgatt
     1561 aaagaattca tgaaggagtg taaagaatga aaacagctta tccaagggtt cccttccccc
     1621 tcattgtaaa ggcgacagat ggcgacgtgg aagcaattaa tcagattgtt aaacattatc
     1681 gagggtatac ctcaaaacgt tcattacgtc gcatgacaga cgaatatgga aattctcata
     1741 tggttattga tgaaacccta cgtggcagaa tggaaacgaa actcatctct aagattttat
     1801 cgttcgaaat taaatagtcc cattttttcg tggaagcgtg tcgaatcgta tccacgcttc
     1861 ccgatttggg aggtttgtta ttccatcaaa gcatattgtg tgttcagtag gttttgatag
     1921 gctaacaagc caaacgttct ttgacaacca aataaaggca atcgaatacg tttcggtaag
     1981 aaaagagctg atgaactaac acgccatgac ctacaaaatt agtgagcgat tcatgttagt
     2041 aattcgaaag taatctttag caggattgct gccatgactt tcttatcgtg ataatgatac
     2101 tcccgtatag ccatagtccg agcgttagaa gcgtcacagg caatgggtac ggctacatga
     2161 gaaccatgca gaagtgaaat tcctgtgagc tttgctagag ctgttcgatt gctaagatat
     2221 tcttctcttg tcagtgtatt attataaaac tatttgatat aattataggt gaatattgga
     2281 gttttcctag cctataattg ggaaaggagt aataatgata acggtcacaa aaaaggattt
     2341 agttgagcta ggatatggaa cttctttttc agcagacatc ataaaaaaag caaaagaatt
     2401 gatgattaca aaagggcata tctactatca atctcgaaag ctagatagag tccctcgtga
     2461 agctgttgaa gaaattctag gaattcggtt ttcaaatgaa aacaggtctg agtagttttt
     2521 gcacagtaag gagtaaaaac tatggttaaa gacccaataa aaaaagcaaa gaatggaacc
     2581 tattattttc gtgcaaatct aggttatgat tctaaaggga aaaaaattca aaaatatcgt
     2641 agtggattca ctactaaaaa agaagcaaga gaagaatatt cgaaactgct attgatgaaa
     2701 ccagaagaat taagtgagaa taaggataaa atgacgttta aacattatat ctttgaaatt
     2761 ttccttccat ggtataagac acaggtaaag ctcagaacat atgaaaatcg cttaccaact
     2821 attaagaagc atttctctta ttttgataaa atggctgttt ccgatattga gcctattgat
     2881 gtgcaaaatt ggcaactaaa attatcaaag aagtgtaagt cttcttatgt aagagctgtt
     2941 caaggactat tttcagttgc tatggatcga gcaattgtcc ttgggataac caccacaaat
     3001 ccatctaaga ttattggcaa tgtgaaaaag caaaagtcta aaattgaatt ttggacaaaa
     3061 gaagaatttg aaaaagtaat ctctctaatt tacaaagaag attactatca acacttttta
     3121 tttatctctc tgtggttttt atttatgact ggaatgcgaa ttggtgaagc tactgccatt
     3181 caatgggaag atattgattt tgactcagga gtactaacca tcaacaaaac actctattac
     3241 aagaatcaat cgaattatag atttgttgaa ccaaaaacaa aggcgagcgt tcgtcatatt
     3301 gtattagata aatgcacatt aacttattta tccgaatgga aggaagtaca acaaaatcta
     3361 attcagacag actttgtaat gagctataac ggtattccta cccaaaaaca tacactatca
     3421 tatgctattg aacgctatgc taagctggct ggtatccatc gaatcagaat ccatgggtta
     3481 cggcattctc atgcttcctt gttaatcagt atgggagaaa atccacttat cattaaagat
     3541 cgtctaggac acgaagatat tgagacgact ttaggaacgt atggacatct ttatcctaac
     3601 agtaatttcg aggtagctaa caaattaaat ggagccattt cctttaaaga agcaaaacaa
     3661 aattatgata cttcaccaaa gaatcaattt actgtagact acttgcgaaa caaagaaatg
     3721 aaaaaagtgc aataaaagtg cagtaaaaag ataagaagag ccagaaaccc ttggtataag
     3781 aggatttcta gctctcctac aactattccc actccactgt tgctggtggt ttagaagtga
     3841 tgtcatacac gatacggttg acgtgggcta cttcgtttac gatacgtaca gagatttttt
     3901 gcaagacttc ccatgggata cgggcaaagt cagcggtcat tccgtcgatt gaagttacag
     3961 cacggatacc gactgtgtag tcgtaagtac gtccgtcacc cataacaccg acagagcgga
     4021 tccctggaag aacagtgaaa tattgccaga tatcacggtc aagtccagca gctgcgattt
     4081 cttcacgcaa gatagcatca gattcgcgta cgatttctag cttgtcttca gtgatttcgc
     4141 caagaacacg aataccaaga ccaggaccag ggaatggctg acgccaaacg attgaatcag
     4201 gcatgcctaa ctgagtccct aattcacgga cttcgtcttt gaataatgtg tttaatggtt
     4261 cgatcaattc aaattgcata tcttctggca gtccacctac attatggtga gatttgatcg
     4321 tctgtgctgt ttctgtacca gattcgataa cgtcagtata caacgttcct tgtgctagga
     4381 aagagacgcc ttcttcacca gcaagttttg ttgcttcgtc atcaaaaaca tatacgaatt
     4441 cattcccaat gattttacgt ttttgttctg ggtcagatac acctttcaac ttgtctaaga
     4501 agcgatctct tgcatcgact ttgatgatgt tcaagccgaa ttttccgcct agactttcca
     4561 ttacttgttc cgcttcgcct ttgcgtagca gaccgtggtc aacaaagata gatgtcaatt
     4621 ggtcaccgat cgctttttgt aaaagaaccc caacgacact agaatcaacg ccacctgaaa
     4681 gaccaagcaa gacttttttg tcaccaactt gttcacggat tttttctact tgcatatcga
     4741 taaagttatc cattgtccag ttaccttcac agtgacaaac atcaaaggca aagtggcgca
     4801 gcaattcgat tccgtattcg gaatgacgta cttctgcatg gaattggata ccataaaaat
     4861 tacgtgttgt gtcttggata gaagcaatcg gacaatcttt gcttgttccg actgtttcaa
     4921 agccttcagg aactttagtt accaagtcac catggctcat ccatactgtt tgttgttttg
     4981 gtgtttcttt aaacaagaca gcttgatcgc ttgttacttc taacaaggct ttcccatatt
     5041 cgcggttttt agcaggctct acggaaccgc caagtttgta cgtgatcaat tgcataccat
     5101 agcaaatccc taatacagga atacctaaat tgaagatctc aggatcgatc ccaaatgcat
     5161 tctcatcata tacactgttt ggtccgccag aaagaatgat tccctttgga ttcatcgctt
     5221 ttacttcttc tgcagtaatg cggtggctca ataattcgga aaagactcca agatcacgaa
     5281 tacgtcttgt gatcaattgg ttatactgac taccgtagtc taatacgata atcttttcaa
     5341 cgttcgtcat atcggcaacg tttgtcaaat ttattacccc tatcccttta aattttatct
     5401 caaaatagtc aaactatttt aaaatgctaa tattttcgca tgaaaatcga atcgatgata
     5461 tgattttcag ttttatggag aattatatcg gctcttcctc gtgtaggcaa gatatattcc
     5521 tgtaaatttt tcaaattcac atttttccat acatcttttg ccattttaaa ggcttcttca
     5581 cgatctccga tagcaaaacg ataataataa ttctctggat tttggaaagc cgtatccagt
     5641 aacgcaccaa aacgatccaa ataccatttt tcgatcagca cgggatcggc atcgacatag
     5701 acagaaaaat caaaaaagtc gctgacgtag atttgctgat tcgcaggtaa ttgaagcgta
     5761 ttgatcccct caacgatcaa gatatcaggc tgctggatca attcgtactt gtctggaatg
     5821 atatcataca catcatgtga gtaaacaggt acttttatct cttcctgacc agattttaca
     5881 cggttcaaga aatcgatcag catctccata tcataacttt caggaaatcc tttacggtcc
     5941 atgattccac gctcttttaa tatacgattt ggataaagaa atccgtcagt cgtgatcagc
     6001 tgaacgtttc ttcgtttgaa ggtacgagac aaaatcgtct gcagcagtct ggctgttgta
     6061 ctttttccaa cggctacact tccggcgatc ccgataataa aaggcggtac ttctacgtat
     6121 ttatgaagaa ataaaccttt actcaagaac aaagattcaa attctttcat ataaagataa
     6181 atcagatgag caagaggtac atagatatct cgaacatcct gcatggaaat gcggtcattc
     6241 aaacttttga tattatctag ttcatcttgt gtcaaaggtg cttgcccatt acgataaaat
     6301 tctcgccatt cttctcttgc cacacggtag tagttcattg gatcattcat attagcctcc
     6361 tgcgttctcc catcaatgga actattttaa cacaaaataa acgcaaaccc caccacttaa
     6421 gagagattgc cgatcttttt gtataatctt tataatagaa agaaacggag tcttattctt
     6481 gacttgcgca cggatacata aaaggataga ctgttgtcat gaaagaaatg agaaggatgg
     6541 aagacatgac aaatcattac tattctgaac gcccagatac ggcgcacgat tttgaacaat
     6601 gggcttttga gttaaaaggc agaacctttc aatttgtaac agattctggt gtattttcac
     6661 gtgaaacagt ggattatggt tcacgtgtcc tgattgatgc tttcgattgg gaagagttac
     6721 ctgatgaagg aaagatctta gatgtgggat gcgggtatgg accgattggg ttagctttgg
     6781 catttgcgac acagcggttt gttgaaatgg tcgacattaa tgaacgagcg gtaagtctag
     6841 cgcaaggaaa cgcaaagcgt aaccaaatcg aacatgttga tatccatcaa tcgaatatct
     6901 atgaagcagt tcatgaagaa acctatgcag ctatcgtaag caatccgccg atccgtgctg
     6961 gaaaaaaagt tgtacatgaa attttgacag gcgcatatcc tcggttgaag aagggcggta
     7021 cactgacaat tgtgatccag aagaaacaag gagcgccgag tgcgcaaaag aaaatggaag
     7081 aaacatttgg taatgcagaa atcgtcacaa aagataaagg atattatatc atcaaaagtg
     7141 tgaaagaata actagctgaa aaatagtgga gcgacgaaag agtggaactg ggcgtgcatg
     7201 tacgggtacg tatcagttct ttttttgttg agaagttttt aaaaacaaag aactattttt
     7261 tcttcaaaaa gaaatagtag gaacagagtc gttgtttttt acgataaaat aaataagata
     7321 cagaacttgt atagaaggct gccaagtcgg tagataaagg aggtttttat caaaaaacat
     7381 actttccttt tttctctatt cttttcttgg gggttttggt acactgatag ggaatttaga
     7441 tagaagggag ccaataggat gaagctgacc atcagagata tcgcagaaat ggcaggcgta
     7501 tcagtaacaa ctgtctcaca gattttgaat aacaaaggaa gcagatttag tgataagaca
     7561 agaaaaaaag tgttgtcgat cgtgaatgaa catcattata agcccgatta tttcgcttca
     7621 aatgtgatca atcgacattc aaagaccatc ggaatgatcg tccctgatgt cactgatttc
     7681 ttcttctcca aactgatcga aggtgtagag agctatttga atcctttggg ctatgtgatc
     7741 atgttatgca attctcgtca tagccaagaa aatgagatca agtatttaca agagctttcc
     7801 caccgctcag tggacggaat cttgctggcc acaccaaata ttttacctga gaaatatgca
     7861 ttagacagcg gtttttatca aaaaatgcca atcattttaa tcgatcgcgg attgaatcgt
     7921 cgggataacg gacggcttat cgtaaaggaa tatgagggcg cctatcaagc tgtttcgctg
     7981 tttattgaaa atggccatac gaagatcggc atgctgaagg aaaccactgg atactatcaa
     8041 ttagaagaac gtttcaacgg atatcgtcat gcgctgaaag atcatggcct gccttttaat
     8101 gggaaatttg ttgaacatgg agagttgact gtccaaggtg gatatactgc atcaaaaaaa
     8161 ttactcaaac ataaagatat cacggctatt ttttgcggga atgatgccat ggcgatcgga
     8221 tgctatcagg cgatcgatga attgggcaaa aaaatcccag aagatatctc agtcatagga
     8281 ttcgatggat taaagctgtc agaatacatg attccgcaat tgacgacagt gcagcagcca
     8341 agttttgata ttggatttta tgctgcacgt ttcctgatcg atacgatcga atttcctcaa
     8401 agaaaagttc cgaacaaagt gttcgaaaca aaattaatta ttcgtgagag cgtaaaagta
     8461 ttgacaaagg gctaactcgg cggtatattg cttatgtaag catttacaaa ggctagttag
     8521 tgcttttttt atttttagta aaccagttta ctaaagtggt ttacctttcc taaaaataat
     8581 gtataatacc gaatgaagac aaggaggatc aggcaatgcg aatggtggat ctgattgaaa
     8641 aaaagaggga tggaaacgag ctgtcgaaag aggaaatcga gtacatcgtc acgaactata
     8701 cgaatggaaa gattccagat tatcaagtca gtgcattatt gatggcaatt ttttatcaag
     8761 acatgacaaa tgaagaaatc acgaacttaa cattggcgat tgctaattct ggtgatgtca
     8821 tcgatttgag ttctcttgaa ggaataaagg tagataagca ttcgacaggt ggcgttggtg
     8881 acacgaccac attgatttta gcccccctag tagctagtgt cggagtgaca gtggctaaaa
     8941 tgtctggacg cggtctaggt tacacaggtg gtacactaga caagctagaa gcaattcccg
     9001 gatttcagat cgagctttcg gatgaagaat ttgtacgcat tgttaatgaa agtaaagtag
     9061 cggtaattgg tcaatcagga aacttggcac cggcggacaa aaaattgtat gccttgcgtg
     9121 acgtcacagc gaccgttgat tctcttccgc tgattgccag ttcgattatg agtaaaaaaa
     9181 tcgcggcagg agcagatgca atcgttttgg atgtgaccac tggagacggt gctttcatga
     9241 agaacatcga agatgcaaga cgtctggcaa agaccatgac gagcattggg aaactggcta
     9301 accgagaaac agtggcggtt atctcagata tgtcagaacc tcttggcgaa gctatcggaa
     9361 atagtctaga agttgtcgag gcaatcgaaa cattacaagg aaatggtcca gaagatctag
     9421 tagaaatgtg ctacgcctta ggaagccaaa tggttgttct tgctggcaaa gctaagacaa
     9481 ccgatgaagc aagaacgttg cttcaagaag ctctggaatc aggtaaagcg ttagcaaaat
     9541 tcaaagaaat gatccaaaat caagggggag acccagcgat cgtggaacat ccagaacgaa
     9601 ttttgactgc acgctacaca atggagcttc ctgctaaaca aagcggagtt gtgtcaaaaa
     9661 tcgtggcaaa tgaattagga attgctgcaa tgatgctagg tgctgggcgt aaaaccaaag
     9721 aagaagatat tgatcacgct gttggattga aactgcataa aaaaattggt gacactgtga
     9781 caaaaggtga gtcactattg acgatctata gtaacgacga agaaatcact tctgtcattg
     9841 aattattata taaaaatatt gaaattggtg aatcggcaat gaagccaacg ttgattcatg
     9901 acatcatcac agaataaagg aaaaaagcgg actttaccgt tttaaagaaa aggggaaaag
     9961 aaatggaact aaaccgaatg atcgaccata ctattttgaa agcagacgca tctaaagaag
    10021 ctgtgatgca aattatcgaa gaagcaaaaa aataccattt ttattctgtc tgcatcaatc
    10081 cagcatgggt tgcgctagcg aaagaacaat tacaaggtga gccagtagcc gtatgtacag
    10141 taatcgggtt tcctctagga gcaaacacac ccgagacaaa agcatttgaa acaacagatg
    10201 ccatcaataa cggtgcagat gaagtcgata tggtcatcaa tatcggtgct ttgaaatcaa
    10261 aggatgatca acaagtccaa aaagatatcg aagcagttgt tgaagcagca aaagatcgtg
    10321 cgcttgtcaa agttattatt gaaacatcgt tattagatag agaagaaatc atccgagctt
    10381 gcgaaatagc aaaagctgca ggcgctgatt ttgtcaaaac atcaactggt ttctcaacag
    10441 gcggagcaaa ggtagaggat gtccgtttga tgcgtgaaac tgtaggacca gaaatgggtg
    10501 tcaaagcttc cggcggtatc cataacgaag aagaagcaat ggcaatgatc gaagctggtg
    10561 ctacaagaat cggaacaagc gcaggagtag caatcgtatc tggttcaact ggtgaaggct
    10621 attaattttt tcaaaggaga agctcatggc agaactcaaa caagaatgga tcaacacagc
    10681 tattgaagcg ttagataaag cctatgttcc ttattcccat tttccggtag gcgcttgtct
    10741 cgtaacggaa tcaggcaaga tttatcaagg catcaacatt gagaatgcat catttggatt
    10801 aacgaattgt gccgagcgca cagctttttt caaagcagta tctgaaggag aaaaaacatt
    10861 tacgcatcta gtcgtagcgg ggcacacccc cgatcctatc tctccttgtg gtgcatgtcg
    10921 acaagtaatg gcagaattct gttcccctga tatgccggtt acgctagtag gagacaatgg
    10981 tgtgacgaag gcaacgaccg ttcgtgcact gctgccttat gcatttactg aaaaagatct
    11041 ataactcatg ttcattcagt cagctgactg ttgaaaaata aaacaattat tttaggaggc
    11101 tttacagaaa tgaaaaaagc aaaattattt ggtttaggtg ctgtcgcact agcagcaggg
    11161 ttatttttag gtgcatgcgg caacaacggc tctactgatt catccggtgg caaaagctca
    11221 tcagacacaa caactgcagc attgatcaca gacacaggtg gtgtagacga ccgttcattc
    11281 aaccagtcag cttgggaagg actagaaaaa tgggggaaag atcaaggact ttcacgagga
    11341 aatgatggct tccaatattt ccaatcttca aatgaatcag attacatccc aaatattgac
    11401 caagcattga atgctggatt caaaacgatt tttggtattg gttataaact aaaaccagcg
    11461 atccaagaac aagcaacaaa caatacaggt acgaactttg ttattatcga tgatgtcatc
    11521 gatggattag ataacgttgt atctgcaact ttcaaagaca acgaagcttc ttatcttgca
    11581 ggtgttgcag cagcttatac gactgaaaca aacgtagtcg gctttatcgg cggtgtaaaa
    11641 ggtgaagtaa tcgatcgttt tgatgctgga ttcaaagccg gtgttgatgc aggtgcgaaa
    11701 gaattaggaa aagaaatcaa agtattgaat caatatgctg gtgacttctc tgctccagat
    11761 aaaggacgtt caattgctca aggtatgtat gcacaaaacg cagatatcat cttccatgct
    11821 tcaggcggta caggtaacgg tgtattccaa gaagcaaaat cattgaacga atcaggcgac
    11881 aaaaaagttt gggttatcgg tgttgaccgc gaccaatcag acgaaggcga atacacatta
    11941 aacggcgaaa agaaaaactt cacattgaca tctacactga aagcagttgg aacagtagta
    12001 gaagatttgg cacaaaaatc agcggacggt aaattccctg gcggcgaaca cactgtttat
    12061 ggattgaaag aagacggtgt tggtctaaca gaaggacaac tttctgacga agcgaaaaaa
    12121 gcagttgatg aagcaaaaga aaaaatcatt tcaggtgacg taaaagttcc agaaactcct
    12181 gaagaaaact aaaaccattt ctatagcctt gttagacaca tgtttgacaa ggctatggtt
    12241 tgattagaac aagaattgtt catttatcca agctgccttg tgacagtttg gataagggaa
    12301 caactccctg aagtaatgtt tgtttcaaaa gaagaacgct cactaagcgt gaatcttagt
    12361 caatcgttgc cctagataga aatcgttagg atgtgaaaaa aaggtggttc aagaaaatat
    12421 tgtcatagaa atgcagaata ttaccaaaga atttggaaca ttcaaggcaa atgacaacat
    12481 caatttacaa gtaaaggcag gagaaattca tgcgttgctt ggagaaaacg gtgctggcaa
    12541 atctacattg atgaacgtgc tttccggatt attagagccg acatcaggga aaattttgat
    12601 gcgtgggaaa gaagtaaaga tcacaagtcc gaccaaagcc aaccaattag ggattgggat
    12661 ggtccatcag cactttatgc ttgttgatgc ctttactgta acagaaaaca tcgtgttggg
    12721 aagcgagcct agtcgcgcag ggatgcttga ccataaaaaa gcacgaaaag agatccaaaa
    12781 agtttctgaa cagtatggat tgtcagtcaa cccagatgct tacgttcgtg atatttcagt
    12841 tggaatggaa caacgggtag aaattttaaa aacactttac cgaggagcag atgtactgat
    12901 ttttgatgag ccgacagctg tattgacccc tcaagaaatt gatgaattaa tcgtgatcat
    12961 gaaggaatta gtcaaagaag gcaagtcgat cattttgatt acgcataagt tagatgaaat
    13021 caaagcagta gctgaccgct gtacagttat ccgccgtgga aaaggaatcg gtacagtcaa
    13081 cgttaaagac gtaacctcac aacaattagc cgatatgatg gttggaagaa cggtttcatt
    13141 caaaacgatg aaaaaagaag cgaagcctca agaagtcgtt ttgtctattg aaaatctagt
    13201 ggtgaaagaa aatcgtggat tagaagccgt gaaaaacttg aacttagagg ttcgtgctgg
    13261 cgaagtactt ggtatcgctg gaatcgatgg aaacgggcag tcggagttga tccaagcttt
    13321 gactggtttg cgaaaggcag aaagcggaca tatcaagcta aaaggggaag acatcactaa
    13381 taaaaaaccc cgaaaaatca ctgaacatgg tgtaggacac gtgccagaag accgtcataa
    13441 atacgggtta gtcctagata tgacattatc tgaaaatatc gcactacaaa cctatcatca
    13501 aaaaccttac agtaaaaacg gcatgctgaa ttattcagta atgaatgaac atgccagaga
    13561 attgatcgaa gaatatgatg ttcgaacaac gaatgaactt gttcccgcaa aagctttatc
    13621 aggcggaaat cagcaaaaag caattatcgc tcgtgaagtc gaccgagatc ctgatctgtt
    13681 gattgttgca aatccaactc gtggactgga tgtaggtgcg atcgaattta ttcataaacg
    13741 tctgatcgaa caaagggaca aatacaaagc agtgttactg atcagtttcg aattagaaga
    13801 aattttaaat gtttcggatc gtattgctgt tatccatgaa ggagaaattg tcggtatcgt
    13861 tgatccgaaa gaaacatctg aaaatgaatt aggtctattg atggcgggct ataccttaga
    13921 agaagcacga aaagagttag aaaagggggc tggcgcacat gaatgatcgc agcgaaaaat
    13981 tccgtaattt attagtgcct gtactttcag ttgttttagg attgatctta ggcgcgatat
    14041 tgatggctgc ttttggtttt cagcccatcc aaggttattc ttctatgcta gatgcagccc
    14101 ttggttcgca aagagggata ggtgaaacgt taaggcaagc aacgccgttg atttttacag
    14161 cattaggttt ttctgtagct aactcagctg ggtttttcaa tattgggctt tctggacaag
    14221 cactttgcgg gtggatcgtc agtgtctggg tggcacttgc gtttccagat ctgccaaaag
    14281 ttgttttgct tccgttatgc gtgatcttag gtgccttagc tggtgcgatg gcagcggctg
    14341 ttccaggttt acttcgagca ttttttggaa caagtgaagt gatcgtaaca atcatgatga
    14401 actatatctt attatacgta agcacatttg ctttacatga agtgatgcct gaaagctatc
    14461 gctcaagttt ggactcttcc aatatgatca gtcaaaatgc ctcactgcgg ttaccatggt
    14521 taacacaagc ttttggcggc tctcgcgtaa actcaggatt atttttagca atcatcgcgc
    14581 ttattttcgt ttggctgctg atgaagaaaa cgactttagg atttgaaatc cgttctgttg
    14641 gattgaatcc ttttgcttct gagtatgcag gaatgagtag taaacgtacg atcgttttgt
    14701 cgatggtcat ttctggcgct ttggcagggc ttggcggtgt ggttgaagga ttaggaacgt
    14761 atcaaaactt ctttgttcaa acgacttcgc tatctattgg atttgacgga atggctgttt
    14821 ccttgcttgg ttctggatca gcaatcggta ttcttttatc agcgatcttg ttcagtattt
    14881 tgaagatcgg tggacttggc atgcagacag gagcaggtgt tccgtttgag atcgtcaatg
    14941 tatcgattgc tttgattatc ttctttgtcg ggatcaactt cctgattcgt ttcgtcatgg
    15001 ctaaagtctt ccaagggaaa aaacaagaag agatcgttgc gacaatagaa tcgaaaccaa
    15061 cgaatcaaaa ccatgaaggg ggcgaactat aatgtcgact atcgaattaa ttgccttgat
    15121 ccttacttct acactggttt attcaacacc gctaattcta acgtcactag gcggaacttt
    15181 ttctgaacga agcgggatcg taaacgtagg gctagaggga atcatggtca tgggtgcttt
    15241 tagttccatc gttttcaact tgacgatgag tccatcactt ggttctatga caccatggat
    15301 cgctatttta gtgggtggcg tcgtaggtat cctattttct ctgattcacg cagtagcaac
    15361 agtcagtttg cgtgctgacc atattatcag cgggacagta attaatctga tggcacctgc
    15421 tttaggtgtt ttcttgatca aagcatggta tggtaaaggt caaactgata acatcacgca
    15481 gaatctgggg tatttttctt tcccaggatt agcagatatt ccggtcattg gaccaatttt
    15541 ctttaaaaat acattcttac ctgcttattg tgctattttg attgctgtcc tagcttgggt
    15601 gatcatcttc aagacgaaat tcggcttgcg cctccgttca gttggtgaaa atcctcaagc
    15661 tgccgacaca ttaggtatca atgtatatgc tatgcgttat tcaggcgtac tgatttctgg
    15721 cttgttaggc ggtatgggcg gtgctgtttt tgctcagtcg atttccggta acttttcagc
    15781 tggtacgatc gttggacaag ggttcatctc catggctgct atgatctttg gtaagtggaa
    15841 tcctttaggg gcaatgggtg cctcattgtt ctttggtttt gctcaaagct tgagtatcat
    15901 tggcgcacaa ttaccagtta tttcttcgat tccgccagta ttgctgcaaa ttgctccata
    15961 tttgctgaca atcattgtgc tggttgtttt cttagggaaa gcttctggtc caaaagcaaa
    16021 cgggaagaac tatatcaaat caaaataatc ttttagcgaa taaaaagggt gggttacggg
    16081 aagtgttttt cgtctcgccc tttttgtttt aaaggcaata aaaataaaaa gaaagaagga
    16141 ataatcatgt ttaaacgcgt acatctgatc gtcatggatt cagtaggaat tggagaagca
    16201 ccagatgccg ataagttcgg tgatgttggt tctgatacat taggacacat tgcgaaagaa
    16261 gcaggattga acgttccaca tttagagcag cttggtttag gaacgatccg tcctcttgaa
    16321 ggtgtggaat cagtgaaaga tcatcaaggc tatgcgacga aattagaaga agtttctgta
    16381 gggaaagata cgatgacagg ccactgggaa atcatgggat tgaatatcaa aactccattt
    16441 cgtgtgtttc ctaatggatt ccctgatgat ctgatcaaac aaatcgaaga attttctggc
    16501 cgtaaagtcg tatgtaacaa accttatagc ggaacagctg ttattgacga ttacggtgaa
    16561 caccaaatga aaacaggcga tttaattgta tatacttctg ccgatcctgt ccttcagatc
    16621 gcggcacatg aagaaatcat tcctttagaa gaattatatc ggatttgcca attcgttcgt
    16681 gatataacaa aagatgatcc gtatatgatt ggccgaatca ttgcacgtcc gtacgtgggt
    16741 gaaccaggag acttcacacg aacaagcaat cgccatgatt atgcgttgaa tccattcgga
    16801 cacacagtcc ttgattcttt gaaagaagca ggaaaggacg tgattgctgt tggaaagatc
    16861 aacgatattt tcaacggaca aggaatcact gaatctgtcc gcacgaaaag caatatggat
    16921 ggcgttgacc agctgctgaa agttatgaaa aacgattttt caggaatcag ctttaccaat
    16981 ttagtagatt ttgatgcatt atacggtcat cgtcgggatg tagtcggcta tgcgcatgcg
    17041 attgaagaat ttgacttacg aattccagaa ttgcttgaag cgatggaaga agacgacttg
    17101 ctaatgatta cagccgatca cggaaacgat ccgacgttta ctggtaccga tcatacgcga
    17161 gaatatgtac cgttattggt ttacagtaaa aaaatgaaag aacaaggggc attgcctcaa
    17221 ggatattatt cagatatttc agcaacgatt gctgagaact ttagtgtttc ttcgactgaa
    17281 aatggcgaaa gcttcttgaa attattaaaa taatcttttt aaataggaga aagcaaatga
    17341 ccaagttaag cgagatgtta caagaaacga cccagtttat ccttgataaa ggagtaaaag
    17401 aaattgattt tggtttgatt ctaggttcag gattaggtga attagcagat gaaatcgaag
    17461 atgctgtaat gatttcttat gatgagatcc cgttctttcc tgtttcgaca gtagttgggc
    17521 atgcaggaaa gctagtttat gggtctctgg caggcaagaa agtgttagcc atgcaaggga
    17581 gattccacta ctatgaaggt cattccatgc aaacagtgac ctatccagta cgtgtgatgg
    17641 ccggactagg ggcccattca gtcattgtga ctaatgcatg tggtggtgtc aacgaatcat
    17701 ttgtaccagg agatttgatg ctgattaccg atcatatcaa ttttactggt gaaaatcctt
    17761 tgatcggtcc gaatgaagat gcaatgggcc ctagatttcc agatatgagt caagcttata
    17821 cgcctgacta tcaagcaaca gcaaaagcag cagcagaaaa actaggtttg gatcttaaag
    17881 aaggcgtata tatgggctat tctggaccaa cttatgaaac gcctgcggag atccgaatgt
    17941 ctcggacaat aggagccgat gcagtaggga tgtcgactgt tccagaagtc atcgtagcag
    18001 ctcacagcgg attaaaagtg ttaggaatct cttgtattac caacttagca gcaggtatgc
    18061 aagcgaatct gagccatgaa gaagttgtag aaacaactca gagagtcaaa caatctttta
    18121 aagcattggt taaagaaaca ttgacattat tgtaatagga ggaacaataa aatgagtgtg
    18181 catattgaag cgaaagaagg cgagatcgcc gataagattt tacttccggg agatcctttg
    18241 cgagctaaat atattgcgga gacttttttg gaagatccag tttgttacaa tcaggtacgc
    18301 ggtatgttag gctttaccgg caaatataaa ggagaacgta tttctgttca aggaacagga
    18361 atgggaatgc catcagccac catctatgcc catgaattga tccagtcgta tggtgtaaag
    18421 aaattgattc gagtaggtac atgcggtgca ttgtcaaaag acgtccatgt tcgtgattta
    18481 gtactagcac aaggtgcagc gaccagttct tcgatgatcg aaaaaaattt ccaagccttt
    18541 cattttcctc caatcagtga tttcaatcta ttactgaaag catatgagat agctaaagaa
    18601 aagggctata cggttcatgt aggaaatgtt ctttcggaag attctttcta caaagatgat
    18661 ttgacagaga cgttccaatt agctgaactt ggcgttttag gagtggaaat ggaagcagcg
    18721 gctctttatt acttaggcgc gaaataccat gtgcagacat taagtttgat gaccgttagc
    18781 gaccatttga tcaccggtga agaaacaaca gctgcagaac gtcaatctac gttcaatgaa
    18841 atgatcgaag taggattgga aacagcgata gcagaataag aaggaaatga aaaagctgcc
    18901 ctgcattaag taataagaac atccaagcaa aaatagctcc tcatgttttt gacttggctg
    18961 gacttattat tgcaaatgta agcttttgaa caccgtttac caagaggttg tgaatctgcc
    19021 gtttaactct gagtcacaag gaacaatttc aaaaaacagc ttttcatgtt tgatgaatat
    19081 tgtgacttgt gaccgagaag ttggcaggag aaaaccgttt atacgaaaaa agggactgtg
    19141 acaaatcgtg tcacagtccc ttttttatag tgttttttat aggtagtatt ttttagcaac
    19201 tgttaagtca tcatttaatt cataaacaag tggttgacct gttggaattt caagatccat
    19261 gatgtcttcg tcagagatac cttcgatatg tttagctagg gcgcgtaatg agttaccatg
    19321 tgcagctact aaaacagttt tgttgtctag taatgctgga gcaatttcat cttgccagaa
    19381 tggcaaagcg cgttctaatg tcactttcaa gttttctcca ccaggaatat cgcgtttgtc
    19441 taacattgcg taacgacggt catttgctgc agaaccttcg tcagtttctt ccattaatgg
    19501 aggaagagta tcgtaagaac gacgccagat gtgcacttgt tcgtcgccgt atttttcagc
    19561 tgtttctttt ttgttcaaac cttgtaattt accatagtga cgttcgttta agcgccatga
    19621 tttgatttgt ggtacccaaa gttgatctga atattctaag atcaagttac atgttttgat
    19681 tgcacgagtt aaaacagaag tgtaagcaac atcaaattcg attccagctt ctttgatttt
    19741 gcgtccgcct tcttttgctt cttcgattcc ttctggtgct agatttacat cagcccaacc
    19801 agtaaattgg ttcaatgcat tccattcact aagaccgtga cgagaaaaaa ctaattttgg
    19861 catgtttagc tctcctttta agataggttt atatttctga aattttcagt tctgttttat
    19921 tgtacaattt tttggaagag atttccatcg aaaaattaga taagattcta tgagaaaaca
    19981 agtagagaat ataaaaaaac gaacagtgcc cacgcaactg ttcgttttta ggtattagat
    20041 gcataattgc tcgatttctt tttcaggtaa attgatatat ttataacatg ttccaactga
    20101 aacaccgcat tttgaagaaa taaattggat cgtttctttt ttttcataat agagacgtcg
    20161 gatttttttg atcgtttttg catcgatttt tggtcttccg ccgatttttc cttttttacg
    20221 agcttcatct aatccaacta atgtacgttc tttgatcaat gcgcactcca tgtttgctag
    20281 gttttccatt aattggaaat agatttttcc catttcgcgc gtatcgattc cttcatcaag
    20341 actaatcaag tgcagcccgc gatctttgaa taattgcgtg agttctgtta attgccgcgt
    20401 agatttgcct aatcgatgaa gttggcaaat gactagtgca tcgccttgtt gcatgtcagc
    20461 gatcactgac tccaaatgaa cagcagtaga atcattttgt tccacttcat atgtctcatg
    20521 gaaaatttct tgacaaccaa agtcagataa ttgatcaacc tgagttgtta aatcaacgtc
    20581 atttatggtt gtgcgtgcat aaccaataat tttcatggtg attcctttct agcagtgatt
    20641 ttctaactgg atataacagt aaaaatgtag cataaagtac gtcaaaaaga tagttttttc
    20701 gcaaattttc cgctgtcttt ttttattgga tgatcaagca gtttttcttt atttacgacg
    20761 gattgcgtca agaatctcaa cgcctttttc tgagccgaca actactttag aaaccatgtt
    20821 taggaaaagc ccatgttcta ctaccccaac aagttcatct aaataggtag ctaagctttc
    20881 tggctgttcg atgacttcta gatgcagatc aatgatgtaa tgaccaccat ctgtggttaa
    20941 gacttctccg ttttcgtctg tacgcaagac gggttggaat cttttttctt caaaaatatg
    21001 aagtagctgt tgggagccat aagggatcac ttcaactggg agtgggaact tcccaagttt
    21061 atcgactaat ttagaagaat caacgatcca aatcgtttct tttgagtacg tagcgactat
    21121 tttctcgaaa agcaaagcag cgccgccgcc tttgatccct tgaaaatcag cactgatttc
    21181 atcagcaccg tctattgtca aatcgactac ggggacttca tcaatactct ttaacggtat
    21241 tcctaatttt tccgcttgtt cttttgtcgc atttgaagta gtaactcctg tgatacgtaa
    21301 accttcttca cgcatacggc gaccaatctc ttctaccata taataggcgg ttgaaccggt
    21361 cccgagacct acgatcatcc cgtcctttac gaatttcgcg gcttcaatgc cagctaattg
    21421 ttttaaattc atctttgcgt ccaccttttt ttagtttgag catcgtgctt tcctcactat
    21481 tttagcgtga tttacggtaa aaagatacca ttctttttta ttccaataaa gctttttcaa
    21541 aaaaaattgc atctcttgtt gacatgtttt ttcattcgtg atattctaac aaaggtgctt
    21601 tatctacagg tttctgtgaa gaaacgtgct gactgtttat caaagcatat ttgataagga
    21661 tgcagacatt gcatttattt tttatcacaa aaaaagaacc acctggatgt gtggaactag
    21721 acgcaaatcg tgggaaggag gaaacaagga atgcctacaa ttaaccaatt agtacgtaaa
    21781 cctcgtaaat caaaggtgga aaaatctaat tcacctgctt tgaacaaagg atataacagc
    21841 tttaagaaaa ctcaaactaa cgtaaactca cctcaaaaac gtggggtagc aactcgtgtg
    21901 ggtacaatga cacctaaaaa accgaactca gctttacgta aatatgcacg tgttcgtttg
    21961 tcaaacttaa ttgaagttac tgcttatatc ccagggattg gtcataactt acaagaacac
    22021 agtgttgtat tattacgcgg tggtcgtgta aaagacttac caggggtacg ttaccatatc
    22081 gttcgcggtg ctttagatac tgctggtgtt aacgaccgta aacaaagccg ttctaaatac
    22141 ggaactaaaa aacctaaagc ttaattttaa ttattaaaca aatgaaactt gcttctacta
    22201 tatagaagta attactatga acaatatttc ggaaggagga gttacggatg ccacgtaaag
    22261 gtcctgttgc aaaacgtgat gttttaccag atcctattta taactcaaaa ttagtaactc
    22321 gcttgatcaa ccgtgtaatg gttgacggaa aacgcgggat tgcagctaac attatctata
    22381 atgcctttga cttgatcaaa gaatctacag gtaacgatcc attagaagtg tttgaacaag
    22441 caatgaaaaa cgttcaacct gttcttgaag ttaaagcacg tcgtgttggg ggttcaaact
    22501 accaagtacc agttgaagtt cgccctgaac gccgcgttac tctaggtctt cgttgggtag
    22561 taaactacgc tcgcttgcgc ggtgaacaca caatggaaga acgcttagca aaagaaatca
    22621 tggatgctgc aaacaacaca ggtgcttctg ttaaaaaacg tgaagacact cataaaatgg
    22681 ctgatgccaa ccgtgcgttt gctcactatc gctggtaaga ttctctctgt ttggtcggct
    22741 tttataagtc gacctcagat agtgtcctta tttttagcaa ctagatagta aagagaggag
    22801 aaacaaggag aatgaccaga gaattttctt tacaaaacac tcgtaatatc ggtatcatgg
    22861 cccacatcga tgctggtaaa acgactgcta ctgaacgtat cttgtactat actggtaaaa
    22921 tccataaaat tggtgaaact catgaaggag cttcacaaat ggactggatg gaacaagaac
    22981 aagaacgtgg tattacgatc acttctgctg ctactacagc acaatggaag ggtcaccgaa
    23041 tcaacatcat cgacacacca ggacacgtag acttcacagt tgaagttgaa cgttcattgc
    23101 gtgtacttga tggtgctgta actgttcttg atgcacaatc aggtgttgaa cctcaaactg
    23161 aaacagtttg gcgtcaagca acaacttatg gtgtacctcg tatcgtattt gcaaacaaaa
    23221 tggacaagat cggtgctgac ttcttatatt cagtatcaac tttacatgac cgtttgcaag
    23281 caaacgctca cccaatccaa ttaccaattg gtgcggaaga cgacttcaca ggaattatcg
    23341 accttgtaac aatgaaggct gaaatgtata caaacgatct tggaacagag atcgaagaaa
    23401 cagaaattcc tgaagaatac cgcgaattag ctgaagaatg gcgcgaaaaa ttggttgaag
    23461 ctgtagctga aactgatgaa gaacttactt tgaaatactt agaaggtgaa gaaatcactg
    23521 aagctgagtt aaaagaaggt atccgccgcg caacagttaa cgtagaattc tacccagtac
    23581 tatgtggttc agcatttaag aacaaagggg ttcaattact attggacgct gttcttgatt
    23641 acttgccatc accgttagat atcccagcta tcaaagggat tgaccctaaa actgatgaag
    23701 aagtagaacg tcctgcagac gacagcgctc ctttctcagc tttagcattt aaagttatga
    23761 ctgacccatt cgtaggtcgt ttaactttct tccgtgtgta ctctggtgtt ttacaatcag
    23821 gttcatacgt acaaaacgca acaaaaggca aacgtgaacg tgttggtcgt atcctacaga
    23881 tgcatgccaa ctctcgttca gaaatctctg aagtttacgc tggagatatc gctgctgctg
    23941 ttggtttgaa agacacaact actggggaca cactatgtga tgaaaagagt ctagttattc
    24001 ttgaatccat ggaattccca gaaccagtta tccaagtcgc tattgaacct aaatcaaaag
    24061 ctgaccaaga taaaatgggt gtggctttac aaaaactatc tgaagaagat cctactttcc
    24121 gtgctgaaac aaacgtggaa acaggcgaaa caatcatcgc tggtatgggt gaacttcact
    24181 tagatatcat cgttgaccgt atgcgtcgtg aattcaaagt tgaagctaac gtaggtgcgc
    24241 ctcaagtatc ttaccgtgaa actttccgtg ctggcactca agccgaaggt aagtttgtac
    24301 gtcagtctgg tggtaaaggt caatacggtc acgtatggat tgaatttaca ccaaatgaag
    24361 aaggtgctgg tttcgaattt gaaaatgcga tcgtcggtgg ggttgttcct cgtgaataca
    24421 ttccagctgt tgaaactggt ttgaaagatg ctatggaaaa tggtgtctta gctggctatc
    24481 cattagtaga tatcaaagct aaactttacg atggttcata ccatgatgtc gattcaaatg
    24541 aaactgcctt ccgtgttgct gcttctatgg cattacgtgc agcagctaag aaagctaatc
    24601 cagtaatcct tgaaccaatc atggctgtag aagttgttat ccctgaagat tatttaggtg
    24661 atgttatggg tcacgtaact gctcgtcgtg gacgtgttga aggtatggaa gctcgcgctg
    24721 gcggccaaca agttgttcgt gcgatggttc cacttgctga aatgtttggt tatgctacaa
    24781 ctcttcgttc tgctactcaa ggtcgtggta catttacaat gacatttgac cactacgaag
    24841 atgtaccaaa atcagtacaa gaagaaatca ttaagaaaaa cggcggaaaa gctgaataat
    24901 ttcttaaaac cggatagtcc tgcaaagaga ttatccaaag agactatatt tttaatatag
    24961 tttcgtgtaa aataattatc gatgatatgg gcatgtggtg taccttgtgt acatcatata
    25021 cctatcagac aaataaaaaa tatattgtat ttttaggagg acattttaaa atggcaaaag
    25081 aaaaatttga ccgttctaaa ccacacgtta acattggtac tatcggacac gttgaccatg
    25141 gtaaaactac attaacagct gcaatcacaa ctgtactatc taagaaaaac ggcggccaag
    25201 ctatggctta cgatcaaatc gatggtgctc cagaagaacg cgaacgcgga atcacaatct
    25261 caacagctca cgttgagtat gaaacagata cacgccacta cgctcacgtt gactgcccag
    25321 gacacgcgga ttacgttaaa aacatgatca ctggtgctgc tcaaatggac ggagctatct
    25381 tggtagtttc tgctgctgac ggcccaatgc ctcaaactcg tgaacacatc ctattgtctc
    25441 gtcaagttgg tgttccttac atcgttgtat tcttgaacaa agtagacatg gttgatgacg
    25501 aagaattact agaattagtt gaaatggaag ttcgtgacct attaacagaa tacgaattcc
    25561 ctggtgacga tgttcctgta gttgctggat cagctttgaa agctctagaa ggcgacgctt
    25621 catacgaaga aaaaattctt gaattaatgg ctgcagttga cgaatacatc ccaactccag
    25681 aacgtgacaa cgacaaacca ttcatgatgc cagttgaaga cgtgttctca attactggac
    25741 gtggtactgt tgctacaggt cgtgttgaac gtggacaagt tcgcgttggt gacgaagttg
    25801 aagttgttgg tattgctgaa gaaacttcaa aaacaacagt tactggtgtt gaaatgttcc
    25861 gtaaattgtt agactacgct gaagctggag acaacattgg tgctttacta cgtggtgttg
    25921 cacgtgaaga catccaacgt ggacaagttt tagctaaacc aggtacaatc acacctcata
    25981 caaaattctc tgcagaagta tacgtgttga caaaagaaga aggtggacgt catactccat
    26041 tcttcactaa ctaccgtcca caattctact tccgtacaac tgacgtaaca ggtgttgttg
    26101 aattaccaga aggaactgaa atggttatgc ctggcgacaa cgtaacaatg gaagttgaat
    26161 taatccaccc aatcgctatc gaaaacggta ctaaattctc aatccgtgaa ggcggacgta
    26221 cagttggtgc cggtgtcgtt acagaaatca aagcttaatt cacttaagtt ttaaagtaaa
    26281 gcaacattta ttcggacgta atcgctcgga acgaaggtct taggatcttt gttccgagct
    26341 tttttattta ttgcaggatc aggaaaaaag acaggagtat tataaataga agaaacacga
    26401 aataaaagag tcagtatttt ctaaatgaaa ctttatgaaa acaaaatgtt ttttacaaga
    26461 aaaagccttg ctttgtgagt ttatatatat tataatgaca aaggtgcgtt tactttagtg
    26521 aattgcacag acgtaatcag aatgatttcg tcggctaaga aacgagaggt tgcgacacgc
    26581 ccggacgctt tgccacgaac gagcgtgacg gaaattttcg tggagctatg tctacttttc
    26641 aaaataggcg aaggagggaa caagatggca aaacaaaaaa ttcgtatccg tttaaaagcg
    26701 tatgaacacc gtattttaga tcaatcagcg gataaaatcg tggaaactgc aaaaagaact
    26761 ggagctagcg tgtctggtcc aattccatta ccaactgaac gcagtcttta cacaatcatc
    26821 cgtgcgactc ataaatacaa agattcacgc gaacaattcg aaatgcgtac acacaaacgt
    26881 cttatcgaca ttgtgaaccc aacaccaaaa actgttgatg ctttgatgaa gctagactta
    26941 ccatctggtg taaacatcga aatcaaatta taaaactaaa acaaaaataa tggaggtgta
    27001 ctcatgacca aaggaatctt agggaaaaaa gtgggaatga cacaaatctt cactgaatct
    27061 ggtgaattaa ttccagtaac tgttgtagaa gctactccaa acgtagtact acaagtgaaa
    27121 acaatggaaa cggatggcta cgaagctatt caagttggtt accaagatat gcgtgaagtt
    27181 ttatcaaaca aacctgcgaa aggtcatgtt gcaaaagcaa acacggctcc taagcgcttc
    27241 attcgtgaat tcaaaaatgt tgagctagga gaatatgaag taggaaaaga aattacagta
    27301 gatgttttcc aagcaggaga cattattgat gtaacaggta ctacgaaagg taaaggattc
    27361 caaggggtta tcaaacgcca cggacaaagc cgcggaccaa tggctcacgg ttctcgttac
    27421 catcgtcgtc ctgggtcaat gggtccagtt gcacctaacc gtgtatttaa aaacaaacgt
    27481 cttgctggcc gtatgggtgg caaccgcgta actatccaaa acttggaagt tgtacgtgtc
    27541 gatgcagaaa gaaatgttat cttgatcaaa ggtaatgttc ctggagctaa aaaatcttta
    27601 attactatca aatcagctgt gaaagctaaa taattgtagg aagaaaggag gaactaagga
    27661 atgccgaatg tagcattatt caaacaagat ggaagccaaa acggtgaaat cacacttaac
    27721 gaagaaatct tcggaattga acctaatgaa agtgttgttt acgatgcaat cgtaatgcaa
    27781 cgtgcgtcat taagacaagg aacacacgcg gttaaaaacc gtagcgctgt tcgcggcggc
    27841 ggacgtaaac catggcgtca aaaaggaaca ggtcgtgcac gtcaaggatc tatccgctca
    27901 ccacaatggc gtggaggtgg cgtagtcttt ggaccaacac cacgttctta cagctacaaa
    27961 cttcctaaaa aagttcgtcg tttagcaatg aaatctgtat tgtcagaaaa agttgctgaa
    28021 aacaacttgg tagcaatcga aggcttgaac ttcgacgcac caaaaacaaa agaattcaaa
    28081 caagttcttg ctaacttatc tatcgactct aaagtattgg ttgttctaga aaaaggtaac
    28141 gactttgctg ctttatcagc acgtaactta ccaaacgttt ctgtagtaac ttctgataat
    28201 gtgagtgtgc tagacgttgt atcaaataca aaagtgttag caacacaaac tgctcttact
    28261 caaattgagg aggtgcttgc ataatgaact tactagacgt aatcaaacgc ccagtgatca
    28321 ctgaaaaatc aatgcttgcc atggatgaca agaaatacac ttttgaagtg gacactcgcg
    28381 caaacaaaac tttagtaaaa caagcggtag aagctgcttt tgacgttaaa gtcaaaaacg
    28441 tgaacatcgt gaacgtacgt gcgaaattca aacgtatggg caaatatgca ggatatacaa
    28501 aaaaacgtcg caaagcgatc gttacattga ctgaagattc aaaagaaatc caattattcg
    28561 aagctgctga ataagcacta atagataact cattaaatta ggagggaaaa acgtggcgat
    28621 taaaaagtac aaacctacct caaatggtcg tcgtaacatg acaggttctg attttgctga
    28681 aatcacaaca tcaacacctg aaaaatcatt gttacaacca ttgaaaaacc atgctggacg
    28741 taacaacaac ggccgcatca ctgtacgtca tcaaggtggc ggacacaaac gccaatatcg
    28801 cgtgatcgac ttcaaacgta acaaagataa cgttgttgca actgttcaaa cgatcgaata
    28861 tgatccaaac cgttcagcta acatcgcgtt agtacactat gaagatggag tgaaagctta
    28921 catcttagca cctaaaggac tagaagtggg tatgaaactt gtttcaggtc cagaagcaga
    28981 tatcaaagtc ggaaatgctc ttccattaga aaacattcca gttggtactg ttatccacaa
    29041 cattgaaatg aaacctggta aaggtggaca attgatccgt tctgctggta caagtgctca
    29101 agtacttggt aaagaaggca aatacgtatt gatccgcttg aactctggtg aagttcgtat
    29161 gatcttagct gcatgccgtg ctacaatcgg ttctgtaggt aacgaacaac acgaattaat
    29221 caacatcggt aaagctggac gctctcgttg gatgcgtaaa cgcccaactg tacgtggtag
    29281 cgtaatgaac cctaatgatc acccacacgg tggtggtgaa ggtaaagctc ctatcggacg
    29341 taaagcacca gtttctcctt ggggtcaacc agctcttggt tacaaaactc gtaacaaaaa
    29401 agctaaatca gacaaactta ttgttcgtcg tcgcaaaaat aaataattac tttgaccatg
    29461 tgtcgcacat tttgagagga ggttcaccat gggtcgtagt ttaaagaaag gaccttttgt
    29521 cgatgagcat ttaatgaaaa aagttgaagc tcaagcaggg gttgaaaaga aaaaagttat
    29581 caaaacttgg tcacgccgtt caacagtttt cccaagtttt gttggatata ccatcgcagt
    29641 ttatgatgga cgtaaacatg taccagttta catccaagaa gacatggtag gacacaaatt
    29701 aggtgaattt gcacctacaa gaacttatcg tggacatgct gctgacgata agaaaacgaa
    29761 acgctaattt gaggggagga taaatccaaa tggcagaaca aatcacatca gctaaagcaa
    29821 ctgcaaaaac agttcgcact tcacctcgaa aagcccgttt agtaattgac cttatcagag
    29881 gtaaaagtgt cgcagatgca atttcaatct tgaaattcac accaaacaaa tctgctggaa
    29941 tcattgaaaa agtattgatg tcagcagttg cgaatgcaga aaataacttt gacttagatg
    30001 ttgaaaactt ggtagtaagc gaagcttttg ttaacgaagg acctacaatg aaacgtttcc
    30061 gtcctcgtgc aaaaggatca gcttctccaa tcaacaaacg tacaagtcat atcacggtag
    30121 tcgtatcaga aaaataagga gggataatca gtgggtcaaa aagtacatcc aattggaatg
    30181 cgtgtaggca tcatccgcga ctgggatgct aaatggtatg ctgaaaaaga gtatgctgag
    30241 ttcttacacg aagatttaag aatccgtaaa tttatttcaa ctaaacttgc tgatgctgct
    30301 gtatcaacag ttgaaatcga acgcgcaaca ggtcgcgtaa acatttcaat tcacacagct
    30361 aaaccaggta tggttattgg taaaggcggt tctgaagtcg aaagcctaag aaaagaatta
    30421 aacaaattaa ctggtaaaag aatccatatc aatatcgtgg aaatcaaaaa accagatcta
    30481 gatgcaaaat tagtaggtga aggaatcgca cgtcaattag aaaaccgtgt tgctttccgt
    30541 cgcgctcaaa aacaagccat ccaacgctca atgcgtgctg gcgctcaagg gatcaaaact
    30601 caagtatctg gtcgtctaaa cggagcagat atcgctcgtt cagaaggata ctcagaagga
    30661 actgttcctc ttcatacttt acgtgcggac attgattacg catgggaaga agcagataca
    30721 acatacggaa aactaggagt taaagtgtgg atctatcgtg gagaaattct tccaactaaa
    30781 aaaaacactg agaaaggagg gaaataatca tgttagtacc taaacgtgta aaacaccgtc
    30841 gtgaattccg cggaaaaatg cgcggggaag ctaaaggcgg aaaagaagta gcattcggtg
    30901 aatacggttt gcaagctgtt gattcacatt ggatcacaaa ccgccaaatc gaagctgctc
    30961 gtatcgcaat gactcgttac atgaaacgtg gtgggaaagt atggattaaa attttccctc
    31021 acaaatctta tactgccaaa gcaattgggg tacgtatggg ttctggtaaa ggggcacctg
    31081 aaggatgggt tgcaccagta aaacgtggta aaatcatgtt tgaaatcgca ggcgttcctg
    31141 aagaagtagc tcgtgaagcg ttacgtctag cttctcacaa attaccaatg aaaactaaga
    31201 tcgtaaaacg tgaggaaatg ggtggtgaat cgaatgaagg ttaaagaaat cagagaatta
    31261 accactgccg aaatgcttga tcaagaaaaa caattaaaag aagaattgtt taatcttaga
    31321 ttccaattag caacaggtca attagaaaac actgcacgta ttaaagaagt acgtaaatcg
    31381 attgcacgca tcaaaactgt gttgcgtgaa caagctaagt aattatggaa ggaggccatt
    31441 aagcgtatga ctgaaggaag aaatcaacgc aaagtttatc aaggtcgcgt ggtttcagac
    31501 aaaatggata aaacaattac cgttgctgtc gaaacaaaga aaaaccaccc tatctatggc
    31561 aaacgcatga actattctaa gaaatacaaa gtgcatgatg aaaacaacac agcaaaagtt
    31621 ggcgacatcg tgagaattat ggaaactcgt ccattatcag ctacaaaacg tttccgtttg
    31681 ttggaagtag tcgaagaagc agttattatc taataacacg agattttcct atatagattg
    31741 aatgcaaaat ctgaaaggag gatacacaac gtgatccaag cagaaagtcg tttaaaagtc
    31801 gctgataact caggcgcacg tgaaattttg acgatcaaag tccttggtgg atctggtcgt
    31861 aaaactgcta acatcggtga tgtgatcgtt gctacggtta aacaagcaac gccaggtggg
    31921 gttgtcaaaa aaggtgacgt cgttaaagcc gttatcgttc gaacaaaatc aggagctcgt
    31981 cgtacagacg gttcatatat taaatttgat gaaaatgctg cagtgattat tcgtgatgat
    32041 aaaagccctc gtggaacacg tatctttggg ccagttgcac gtgaactacg tgaaaacaac
    32101 ttcatgaaga tcgtttcact agcaccagaa gttttataat cttgagacac ttatcaaagg
    32161 aggtgcgaaa cagttatgtt tgttaaaaaa ggcgataaag ttaaagttat cactggaaaa
    32221 gacaaaaaca aagaaggcgt tgtactagca gcgttcccga aaaaagataa agttattgtc
    32281 gaaggtgtaa atgtcgttaa aaaacaccaa aaaccttctc aagctgctcc tcaaggtgga
    32341 attgttgaga tggaagcgcc aattcatgtt tctaacgtga tggtggttga ctctactggt
    32401 gtagctggcc gagttggcta caaagaagta gacggcaaaa aagtccgtgt ttctaaaaaa
    32461 accggtgaag ttttagataa ataaaagttt aaggaaggag ggtcttactg aatgaaccgc
    32521 ctaaaagaaa aatatcttaa agaagtaact ccatcattga tggaaaaatt taactatagc
    32581 tctgttatgc aaacacctaa agttgaaaaa atcgtcatca acatgggtgt tggtgatgcg
    32641 gtatcaaacg ctaaaaactt agataaagca gttgaagaat taactttgat cactggtcaa
    32701 aaaccaatga ttacaaaagc taaaaaatca atcgctggat tccgtttacg tgaaggaatg
    32761 ccaattggtg caaaagttac attgcgcgga gaaagaatgt acgaattctt agacaaattg
    32821 gtttctgttt cactaccacg tgtacgtgac ttccatggtg taagtaaaaa agcctttgac
    32881 ggtcgtggaa actatacttt aggtattaaa gaacaattga tcttcccaga agtagattat
    32941 gatttagtag ataaagtacg tggtatggac atcgttattg taacaacagc aaactctgat
    33001 gaagaatctc gtgagttgtt gacacaatta ggcatgccat tccaaaaata attaaaggag
    33061 gcgaactacg tggctaaaaa atcaatgatt gctaaaaaca aacgtcctgc aaaattttca
    33121 acacaagctt atactcgttg cgaacgttgc ggacgtccac attcagttta tcgtaagttc
    33181 catctttgcc gtatttgctt ccgcgaactt gcctataaag gtcaaattcc cggcgtgaag
    33241 aaagctagct ggtaaaaagt aaattcgcat aagaaggagg taaaagatca atggtcatga
    33301 cagatccaat tgcagatttt ctaactcgta tccgtaacgc aaacatggtt aaacatgagt
    33361 ctttagaagt acctgcatca aaaattaaac gtgacatcgc tgaaatcttg aaacgtgaag
    33421 gtttcgttcg tgatgttgaa tacatcgaag atgacaaaca aggcgtgatc cgtgttttcc
    33481 taaaatttgg taaaaacggc gaacgtgtta tcacaaactt aaaacgtatc tctaaaccag
    33541 gtttgcgtgc ttatgtcaaa gctgacgaag taccaaaagt attgaacggt ttaggtatcg
    33601 caatcatttc gacttctgaa ggtgttgtca ctgataaaga agctcgtgaa aaaaatatcg
    33661 gcggcgaagt tatcgcttac gtatggtaat caagtagaat ctacaaggag gtgtctttaa
    33721 gtgagccgta tcggaaataa agtcgttgtt atccctgaag gagtaacagt tactcaagac
    33781 ggaaacaacg ttacagttaa aggaccaaaa ggtgaattga ctcgtacttt ttcagcagat
    33841 atcaaaatga atatcgaagg aaacgaagta actttcactc gtccaaacga cagtaaagaa
    33901 atgaaaacaa ttcatggaac aactcgtgcg aacttcaaca acatggttgt aggtgtcagc
    33961 gaaggtttcc aaaaagcgtt agaactaatc ggggttgggt accgtgcaca attacaagga
    34021 tcaaaacttg tattgaacgt tggttactca catccagtag aaatcacacc accagctgga
    34081 gtaactgttg aagtaccagc taacacacaa gtaattgtta aaggtgcgaa caaagaagaa
    34141 gttggcgaat tagctgcaaa cattcgtgga actcgtcctc cagaacctta taaaggtaaa
    34201 ggtattcgtt atgttggcga atatgtacgc cgtaaagaag gtaaaactgg taaataatcg
    34261 tttagaaaaa ttgcatagaa gccagcagca ggaaaaaggc atttggctga agtttacctt
    34321 caaaaaaata catttttact tgtgccgcag cttctatttt tcgaaacgta tttactgctt
    34381 gaaatagtag tacaataaaa ataaagaggt gacaattgtg attacaaaac cagataagaa
    34441 caaaacacgt caaaagagac atcgtcgtgt acgtaacaat atctctggta ctgctgagcg
    34501 cccacgcttg aacgtttttc gttctaacaa aaacatctac gctcaaatta ttgatgacgt
    34561 agcgggtgta acgctagcaa gtgcctctac cttggataaa gaaatttcag gtggaacaaa
    34621 aacagaagca gcacaagctg tcggtaaact agttgctgaa cgtgcagctg aaaaaggcat
    34681 taaagtagta gtctttgacc gtggtggata cctttaccat ggccgtgtgc aagctttagc
    34741 tgaagctgct cgcgaaaatg gactagaatt ttagaagaag gaggaatacc attcatggtt
    34801 tatatcgatc caaaacattt ggaattagaa gaccgcgttg ttgcaattaa ccgtgtaaca
    34861 aaagttgtta aaggtggacg tcgtctacgt tttgctgctt tagttgttgt cggtgacaaa
    34921 aacggacacg tagggtttgg tacaggtaaa gctcaagaag ttcctgaagc aatccgtaaa
    34981 gcaattgaag atgcgaagaa aaacttagtt gaagtaccta tggttggttc aacaatccca
    35041 cacgaagtaa tcggtgcttt tagtggtggt agaatcctaa tgaaacctgc tgtagaaggt
    35101 tctggggttg ctgctggtgg accagttcgt gccgtattag aattagctgg ggtagcagat
    35161 atcacttcta aatctttagg ctcaaataca ccaatcaacg ttgttcgcgc aacagttgaa
    35221 ggtttgaaac aattgaaacg tgcagaagaa gttgcagaac ttcgcggcaa atcagttgaa
    35281 gaattaatcg gataaggagg acaacaataa tggctgaatt aaaagttact ttaaaacgca
    35341 gtattatcgg acgtcctcaa aaccaacgcg atacagttaa agcgttaggt cttacaaaga
    35401 ttaacagttc agtggttaag ccttctaatg aagcaattaa aggcatgatc aatactgttt
    35461 ctcacctagt ggacgttgaa gaaatttaat ttagaaaaat cataactaga acattaagga
    35521 ggtgccaaac ctatgaaact tcatgaatta aaacctgctg aaggttcacg ccaagtacgt
    35581 aaccgtgttg gtcgtggtac ttcatctggt aacggaaaaa ctgccggacg cggacaaaaa
    35641 ggacaaaaag ctcgttcagg cggcggtgtg cgtttaggat ttgaaggggg acaaacacca
    35701 ttgttccgtc gtttaccaaa acgaggattc acaaacgtga accgtaaaga ctatgcagtc
    35761 attaatcttg atgtattgaa ccgctttgaa gacggagctg aagtaactcc tgttgcttta
    35821 gttgaagctg gaatcgtgaa aaacgaaaaa gctggaatca aagtattagc taatggggaa
    35881 ctaaacaaga aattaactgt gaaagcagca aaattctcta aagcagctca agaagcaatt
    35941 gaagcggctg gtggatcaat cgaggtgatc taatgtttag actattaaaa gatgctttta
    36001 aagtaaaaga cattagatcg aaaatcctat ttactgtatt tgtgttgttt gtatttcgcc
    36061 taggtgcaca tattactgta ccaggcgtaa atgctgcttc attgcaaaac ctttcttctt
    36121 tgccattttt caacatgttg aacttggtaa gtggtagtgc gatgcaaaac ttttccattt
    36181 tctcaatggg ggtttcacca tacattactg cttctatcgt cattcagctt cttcaaatgg
    36241 acatcgtgcc tcgatttgtt gaatggtcga aacaagggga agttggtcgt aaaaaactga
    36301 atcaagcgac tcgatatctg acattagtat tggcatttat ccaatctgtc ggtatcactg
    36361 ctggattcaa tcaatacgct caattaggtt tcgtgaataa tcctaacgta gcaacttacg
    36421 taacgatcgg tttgattcta acagccggta cgatgtttgt tacttggcta ggagaacaga
    36481 tcacagaaaa aggtatcgga aatggcgtat caatgatcat ttttgcaggt atcatttcta
    36541 gattgcctga aagtatcaga gaaatttatg aagattactt tataaatatt gatccttcag
    36601 acatatggaa atctgctatt tttgtggcca ttttactagt agcggtcttg ttgatcgtga
    36661 cattcgtaac atttttccaa caagctgaac gcaagatccc gatccaatat acgaaacgcg
    36721 tagctggcgc gcctacaagc agctatctgc cgttgaaagt aaatgcagct ggagttattc
    36781 cagttatctt tgctagttcg tttattgcta cgccaaatgc tgttttacaa gcattgagcg
    36841 gtagattcgg ttctgaaaac tggtacaaag tgatgatgga gatctttaac tataatacag
    36901 cacctggtgc tacgatttat acgattctga tcgttgcctt cactttcttc tacgcctttg
    36961 ttcaagtaaa ccctgagaaa ttagcggaaa acttgcaaaa gcaaggaagt tatataccaa
    37021 gcgtacgccc tggtaagggg actgaagagt atgtctcaag attactgatg cgtttaagta
    37081 cagttggtgc agtcttcctt ggtttagtgg cattgcttcc aatcattgct caaatggttt
    37141 gggatcttcc acaatcaatc ggattaggcg gtacaagctt actgatcgtt atcggtgtag
    37201 ctttagaaac tgcgaaacag ttagaaggct taatgctgaa acgtaaatat gttggattca
    37261 ttgagtaaga aagcgtgttg aggtttcccc tcaacacacg ttctcacaca tttattagga
    37321 gggaaaacaa tgaacctcat tttaatgggg ctgccaggcg caggtaaagg aacacaagct
    37381 gaaaaaatca ttgatgctta tggtattccg cacatctcaa caggagatat gttccgcgca
    37441 gcgatgcaaa acgaaacagc tcttggcctg gaagcaaagt cttatatgga taaaggtgca
    37501 ttagttcctg atgaagtaac aaatggaatc gtgaaagagc gactagctga accagataca
    37561 gagaaaggct tcttattaga tggctttcca cgtacgctag atcaagcaaa agctctggac
    37621 gcaatgctaa aagatttgaa taaaaaaatt gatgctgtca tcgatatcca tgttggtgaa
    37681 gaaatcttgg tcgaacgtct agccggccga tttatctgca gcaattgtgg agcaacttac
    37741 cataaaattt tcaaccctac aaaagttgaa gacacatgtg atcgttgcgg tgggcatgaa
    37801 ttctatcaaa gagaagatga taaacctgag acggttaaaa atcgtctggc tatcaacatc
    37861 aaaaatagtg aaccaatctt ggcttattat aaagaacaag gtttgctaaa cactattgat
    37921 ggtgctcgtg aaatcgatgc tgtatttgca gatgtacaaa aaatcattga aaaatagcac
    37981 gattgcccgt agccgacttg cattatgcag ttactcttgt caagttcaat aaattatgat
    38041 agaatatgtc agtccgactt ctagagaatc ctctagatga tccattttga gaattttgag
    38101 gtgggaactg cagtttccgc cattttatcg tgtgaaaatg cgaaacaagt acaaggaggt
    38161 actgtgcgtg gcaaaagaag atatgattga agtcgaaggt acagtcgtcg aaactttgcc
    38221 gaatgcaatg tttaaagttg aactagaaaa cggacaccaa gttcttgcta ctgtttcagg
    38281 taaaattcgt atgcattaca tccgtatctt acctggtgac aaagtaacgg ttgaattgtc
    38341 tccatacgat cttaaccgtg gccgtattac ttatcgcttt aaataattgt actccgtaaa
    38401 actacaggag gtattatcat gaaagtaaga ccatcagtaa aaccaatgtg cgaacattgt
    38461 aaagtaattc gtcgtaaagg acgcgttatg gtgatttgcc cagcaaatcc aaaacataaa
    38521 caacgtcaag gataattgga ggtgctataa atatggctcg tattgcagga gtagatattc
    38581 ctcgtgataa acgtgtagta gtttcactta cttatatcta tggtattggt aacactactg
    38641 ctaaaaagat cttagctgat gctggtgtat ctgaagatgt tcgcgttcgt gaattaacga
    38701 atgaacaaac agatgctatc cgtgcagaag tagataaatt aaaagttgaa ggggatcttc
    38761 gtcgtgaagt gaacttgaac atcaaacgtt tgatggaaat cggttcttac cgtggaatcc
    38821 gtcatcgccg tggattgcct gttcgtggac aaaacacgaa aaacaatgca cgtactcgta
    38881 aaggcccagc tcgcgctatc gctggcaaga aaaaataatt cttaagtgaa ggaggttaaa
    38941 acttcatggc agcaaaaaaa gtgaatcgta aacgccgtgt gaagaagaat atagaagcag
    39001 gtattgcaca tatccattct acattcaata acactatcat catgatcact gatactcacg
    39061 gaaatgcatt agcgtggtca tcagctggtt cattaggttt caaaggaagc aaaaaatcaa
    39121 ctccttttgc cgctcaaatg gcagcagaaa ctgctacaaa agcagcaatg gaacacggat
    39181 taaagactgt tgaagtaaca gttaaaggac ctggttctgg acgtgaagca gcaattcgtt
    39241 cattacaagc tacaggttta gaagtgactg caattcgtga cgtgactcca gttcctcata
    39301 atggtgcccg ccctccaaaa cgccgtcgtg tttaatgagt gccgctcatt ttgagtttta
    39361 tcagtaacgt cattgaacaa gacacttttc gttttgaaag gggtaaagat aagaatgatt
    39421 gagttcgaaa aaccaagaat cgcaaaaatt gatgaagaaa aagattatgg caagttcatc
    39481 gttgaacctc ttgaaagagg gtatgggact actttaggga attccctacg tcgtatttta
    39541 ttatcttctt tgccaggtgc tgccatcact aatattcaaa tcgatggtgt actgcatgaa
    39601 ttctctacca tcaaaggtgt tagagaagac gtcactcaaa tcatcttgaa cattaaaggc
    39661 ttagcgctga aaatgtatgg ccaagaagaa aaaacccttg aaatcgatat taccggacct
    39721 gctacagtaa ctgctggcga tattatcgtt gatagtgatg tagaaattct taacaaagat
    39781 atgtacatct gtagtgtctc tgaaggagct acgttccatg ctcgcttaac agtgaaacct
    39841 ggacgtggtt atgttcaagc agatgaaaat aaaaaagaag atatgccaat tggcgttctt
    39901 ccagtcgatt caatctacac acctgttcgt cgtgtgaact atcaagttga gaacacacgt
    39961 gttggacacc gtgaagattt tgataaattg acgatggaga tttggacaga tggttcaatt
    40021 gaaccgttgg aagcaatgag tttgtctgcg aaaatcatga ctgaacattt ggatattttt
    40081 gtgaacttaa ctgatgaagc aaaaaatgct gaaatcatgg tcgaaaaaga agaaacacaa
    40141 aaagaaaaaa tgttggaaat gacgatcgaa gagttagact tgtctgttcg ctcttacaat
    40201 tgtttgaaac gtgcaggaat caatactgta caagaactta caaataaatc tgagcctgaa
    40261 atgatcaaag tccgtaactt aggacgtaaa tcattagaag aagttaaagc aaaattgcac
    40321 gacttaggtt taggtttacg taaagaagat taaaaccttt tacgaaggag ggaaaccaac
    40381 gtgagttatc gtaaactagg acgcacatct agccaacgta aagcgatgtt gcgtgattta
    40441 acaactgatt tattaatcaa cgaacgtatt gtgactactg aagctcgtgc gaaagaagtt
    40501 cgctcaactg cagaaaaaat gatcacttta ggtaaacgcg gagatttgca tgcacgtcgt
    40561 caagctgcgg ctttcgtacg taatgaagtt gccagcgtgc gtgaagaaaa tgaggacatc
    40621 gtcattgaat cagctttaca aaaactgttc aacgatattg cacctcgtta cgcagaacgt
    40681 caaggcggat acacacgcat tttgaagaca gaaccaagac gcggtgatgg tgcaccaatg
    40741 gttgtcttag aacttgtcta aaatcacact aataacatca ctttatagat gattctttta
    40801 gagtgttatg atgttggaag atatttttcc gagtctagct cagcgctgct cctcaagcga
    40861 atatcgcttg gggagtaagc actcatcata aagtgttttt ttataccttc aattacctgt
    40921 catgacagat atgtttgaag gcataaaaaa acattttaag cgagaaataa ttgaaaattt
    40981 tgtctttttt tgatagtgtt agtaggaact ttaggatgaa gaaggagtaa tcatggagcc
    41041 aattattgaa ttagaaaaga taaattataa ataccagcca gatgatcttc gccccgcatt
    41101 gaaagatgtc tcctttacaa ttgataaagg tgagtggatc gctattattg gacacaatgg
    41161 ttcaggaaaa tcgacacttg ccaaaacgat caatggattg cttctgccag aatcagggat
    41221 cgtgaaagtg ggaaatcaaa tattggatga agaaaatatt tggacgatcc gacagatggt
    41281 tggaatgatt tttcaaaatc cagacaatca gtttgttggt tcgacagtag aagatgacgt
    41341 tgcttttggt cttgaaaatc aagggattcc cagagaagaa atgctagtgc gtgtgaaaga
    41401 tgcacttgag aaagttagaa tggctgagtt tgcttcacgc gagcctgctc gtttatcagg
    41461 agggcaaaaa caacgtgttg ccattgccgg cgtggttgcc ttacgacctg atatcattat
    41521 tttagatgaa gcaaccagta tgctagatcc agaaggtcgt gaagaagtga tctctacgat
    41581 caagaagatc aaagaagaaa gtcaattgac cgttatatct atcactcatg atattgatga
    41641 agcagctaat gccaatcgga tactagtgat gagacaaggc gaacttgtcc gtgaaggtac
    41701 accaaaagaa attttttctg ctggtcctga actgatcgac ttaggtctag atctaccatt
    41761 tcctgaaaaa ctgaagagcg cactgaaaga acgtggggtt gatgtgccca gtgaatatat
    41821 gacagaagaa aggatggtgg attggttatg gacatccgtt ttgaacaagt agacttcact
    41881 tatcagccga atacaccatt tgaacaaaga gccttatttg acatcaatat gacgatcaaa
    41941 gaaaacagtt atactgcatt agtagggcat accggaagcg ggaaatcaac cttacttcag
    42001 catttgaatg cgcttgttaa gccaacaagc ggtacagtcc atattggaga acgagatatt
    42061 cagccggata cggataataa gaatttaaag cccatccgaa aaaaagttgg catcgtcttt
    42121 cagtttccag aagcacagct attcgaagaa acggtagcaa aagacattgc tttcggtcct
    42181 aaaaactttg gtgtcagcga agaagaagca ctagtcctag caaaagaaac attagaacaa
    42241 gttgggctgg atgaaagcta tttggaacgt tcgccatttg aactttcagg agggcaaatg
    42301 cgtagagtag caatcgctgg tgtgcttgcc atgaggccgg aagtacttgt attggatgaa
    42361 ccaacggcag gacttgatcc ccagggcaga aaagaaatga tggagatgtt ttggcgtctt
    42421 cataaggaac agcagatcac tattgtatta gttacgcatt taatggatga tgtagccaat
    42481 tttgcagatt atgtctatgt gttggaaaag ggacgaatcg ttaatagcgg ggagccgcaa
    42541 gaggtctttc aaaacattga atggctaaaa gaaaaacaac taggcgtacc tactgccacc
    42601 gagtttgcag aagaattaat ggctaaaggg atgaactttg ctaccttgcc tttgacagct
    42661 gaagaattag cagacgctat cgtccattat gctggaggaa atgcccatga tgaataaatt
    42721 gatatttggt cgttatatgc ctggagattc cttgatccat cgattggatc cccgagctaa
    42781 attattagcc agtttttatt ttatcggaat cattttttta gcaaataatt gggaaacgta
    42841 ccttttgttg gcatttttca cattactttc tgtcttcttg tcaaaagtag atttgaaatt
    42901 ttttatccga ggcattcgcc cattgatctg gctaatcctc ttcactgtag ctttgcaggt
    42961 cttgtttaca agtggcggag aaacttattg gagttggggt gttttccata tcactgaatt
    43021 tggtgtgatg aacggcctct ttattttctg tcgattcgtg ttgatcattt ttatgtcgac
    43081 tctactcacg cttactacac cgcctttgga attatcggat gcgattgagt acttgcttcg
    43141 tccattgaaa gccgtacggt tcccagtcca cgaaatttca ttgatgctgt cgattgctct
    43201 tcgttttgtg cctacattga tggacgaaac agaaaagatc atgaacgctc agcgcgctcg
    43261 cggtgttgat tttggtgaag gaaacctagt tcagaaaatg aaagcagtca ttcctttgct
    43321 gattccttta tttgtcagca gcttcaaccg tgcggaagat cttgcgactg ctatggaagc
    43381 aagaggctac aaaggtgggg aaggaagaac gaaatatcgt gtattgcatt ggcataatag
    43441 agacacgatg gttatcattg cttttgtatt attaactgtg ctactgatct ttttaagagg
    43501 ataaacgttc agtgggccgc tatctattct tttcttgctg atctgtttgc aagaaaggat
    43561 gcaagcagtg tatgacaaga aatattaggg tctgtggcag actaaggtca caggcctctt
    43621 tcttttatct gaaaagaaag aacgaaaaaa ttttatagaa aaatcattat tccagataac
    43681 cttttaggaa caaaggagag aaaaacatgg tacgttacaa agcaatcatt gcgtacgacg
    43741 gaacaaattt taatggattc caaaaacagc caaatgggcg tactgttcaa gaagaagtag
    43801 aaaaaacatt acaaaaaatg gcaaatggaa aagaaatcac ggtctttggt tcgggaagga
    43861 cagatgccgg agtacatgcg atcgggcaag taatccattt tgattatcca gaggagcgtc
    43921 cgttagaacg aatgcgtttc gcgctagata cccagtcacc agaggatatt gctgtaagac
    43981 aagtagaaat tgtttcagaa gacttccacg caagatattt ggtcaaagaa aagacgtatc
    44041 aatttcgagt ggatataggg aaaccacgca gtccttttcg acgccattat gcaagttatt
    44101 ttccttaccc acttgatttg gaaaaaattc agcgggcgct tcctgattta ttagggactc
    44161 acgatttcac ctctttttgt gcatcaggaa gctcgataga ggataaaata cgtacgatct
    44221 acgaggctaa gatggaggta aatgaaacaa aagacgaatt gttgtttaca tttaggggaa
    44281 atggcttctt gtataagatg atacggatct tagtaggaac gcttttgaaa attggcaatg
    44341 gccgattgcc tgaagatagt atcccagata ttattgcgaa aaaagatcgt aatgcagcag
    44401 gacctacagc acaccctgaa ggactttatt tgtatgaagt gaaatatgaa taagaggtta
    44461 tgatagaagt ggtcagcttc aagaaataag aaggtattta cgaaaattac ttctcaaaat
    44521 tttgcaaaat ttcggcttat tttcgaagaa gctacttctg gaacaccgtt taagaaagag
    44581 gtcgtgagcc tgtcatttaa ctctgagtca caaagagcaa tttcaaaaaa cagcttctca
    44641 tctttgatga atatcgtgac ttgtggacga ggcgttgata agtgaatcat ctttttgtta
    44701 cctatttttt ctgtaaccaa aaaacgatgg aacgttgttt tgattgccct tggtgcggac
    44761 agtctgaaca ggttccatcg tttctttatt ttctacgtct tcttctgtgt gtgtttcgtg
    44821 gttgttcttc tattgctgga tcaatcgtgc cataaaaggg gcgattgcgg tcaaggtacc
    44881 agacataaag aataaacaat gcaatacata ggacaaaaag gattgcacct atcgtaaatc
    44941 cttcaggcaa gtaactgaaa gtgacagtat gtttacctgc tggaactttt atcatcaaga
    45001 atgcgtcttt gaaattgctg accttgactg gtttcccatc aattttagct ttccatcctt
    45061 tgtcataagg aatagttgtc accaacatgg aatcttcagc ggtatttaca gtcccagaag
    45121 cttttctgcc agtggcttcc atttcgacac ctttttcttt gatctgattc attgcggctt
    45181 gataggctac aacatctaga ccaacgactt taggctccat gaaacttatt tctttcgtcc
    45241 cataaaaact tgcagtaaat gtcactgttg tctcttgagg ataatagcca agatcatagt
    45301 attgaccaga aatgttgatt tgcgtttttc gcgatgtacc attgacagtg atcgtcgcac
    45361 tagaactttc taattgcgca aaattggttg ggaacaaact taagtaagct tgtgtatttg
    45421 caggcacatg gacttcccaa gtgagctctt ttggctggtt atctgctgtt tctgagtaag
    45481 tgacgccagt cgttgtcttt gtgatcgtcg tattcaactg attcttcaaa gtaggtgtat
    45541 agaatgtaaa atactgttca tttagaccag acaatgtatt gatcaagttt gtctgactgg
    45601 tcaaattatc tgtcactggc tgctcaacat cattaacgga caacggtgca gtaaatgcta
    45661 gtggcaatgc attgacattc tgatacagtg tatagtcgcc gctttgacca gcttggcgga
    45721 agccatattt actgaacgtg ctttcgtttt tagaaatgtt atatttgatt cctgtaaacc
    45781 catccatcag caaagtatta ttgttataac ggatattcaa atttgtccct cttgaacgga
    45841 aacctaattt atccaggtaa gaggaagaat tacgatttct tatagaagaa aacaaactga
    45901 taccactgta accatagttg atcgaatcgt ttgcagatac tggatccaga ttttccaagc
    45961 ggtaaaacga gtcggaatct ttttttgttt catttacaag agctttcaag tcaggataag
    46021 gatcagtata taaactgcgg gaagcataat tccaatcatc aagaatgcca tggatcatac
    46081 tgttcgtatt gaccagtgct tcagcagaca tgatcaataa taacaatata ctcagatgat
    46141 tgaatgaaag ttttttatgc tgataaaatc ccacagcaaa agcataaatc cctaagaaaa
    46201 ccactgtcaa tacaaaggaa gtcatcgtca cataggtgta gcttccttca ggtttgatgc
    46261 cccatgccac cgcaaagaga gcagctacga ccaaaatgat tcctaacaac agcccaccat
    46321 cgtcttgttc tatcttttcc catccttttc ctgcaagcag gagcactaag aaagagaaca
    46381 gataggcata acggaataaa aacatattcg gtgcatgcat accatgccag aacaaattca
    46441 atggaacaag ataaaaactt gcgataagta tggcgaaaag actgccgtac aaaaatttat
    46501 ttttcaacgg tactttccga ctggcaaaat aaaacagaca gaggactaat ggtagcagac
    46561 ctgcgtaaac gaacggaatc gatccgtatt ttgtcgtgtc ataaacacca atcatatttt
    46621 tcatgattag atccagataa gaagtcgctt ctgttttcaa cgtagtgatc tgactcagtg
    46681 cctcgccatt tgaccgaaga tccagcactg ctggcaatac aatgatcatt gaagcgccgc
    46741 ctgccagtaa agaagtgata ccataaggaa caatcgtttt tttatattct gaccagtttg
    46801 tcaatagacg ggcgataaaa tataagaaag aaaatacgcc aatcataaaa cccatataaa
    46861 aactggataa aaacaagagt aaataactga taaataaaag tttcggccgt ttaagatcca
    46921 taacacgatg aatccccaaa atcactaatg gaagataaac aaatgcatcc agccacatga
    46981 tgatctccga atgtgcagtg ataaatgaga tcaatgcgta actgacgcta agtgcaacat
    47041 gcccccagcg aggaatcttg aacgtacgtg caagaaacca aaaagccaat ccggcacagc
    47101 cgaccttaag caaagtcaga acatacagtg catctggcat catctggttt ggaaaaaaga
    47161 acaccagtgg cgtgaagatc ccgcctagat agtaagaaat taaagataaa taattcaatc
    47221 ctagagaagc attccatgta taaaaaatac tttgctttcc aagaagaaca tttcggaagc
    47281 tggcatgaaa attagaaaac tgtgaaaagg catcacttgc caaaatactt ctgctgcttc
    47341 caggatagat tccgatactt agataaacaa gtgctaaaat caaaaacgga atcaggaaac
    47401 tagctgctgc gtagcgtccg ttttctctaa agaatgactt taaatttttt ttcatattta
    47461 ttttccacca aatttctatg aagttgtatt cagtttatca tatccttgtt tttctctgct
    47521 taaaaagagt ctagttttca caaaaaaata aaatttagaa taaataactt cataatttgt
    47581 tcgtataaca aaaataaacg caaaaaaagg gtaggaatca aaagtcccac ccttaa
//
LOCUS       DAZUMM010000011        46028 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000011 DAZUMM010000000
VERSION     DAZUMM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.011"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..837
                     /locus_tag="ACG8E6_000580"
     CDS             157..837
                     /locus_tag="ACG8E6_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS5061027.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            927..1961
                     /locus_tag="ACG8E6_000581"
     CDS             927..1961
                     /locus_tag="ACG8E6_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS5061028.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1975..2310
                     /locus_tag="ACG8E6_000582"
     CDS             1975..2310
                     /locus_tag="ACG8E6_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061029.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2294..4774
                     /locus_tag="ACG8E6_000583"
     CDS             2294..4774
                     /locus_tag="ACG8E6_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061030.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4830..6389
                     /locus_tag="ACG8E6_000584"
     CDS             4830..6389
                     /locus_tag="ACG8E6_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5061031.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6386..7750
                     /locus_tag="ACG8E6_000585"
     CDS             6386..7750
                     /locus_tag="ACG8E6_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS5061032.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7921..8991
                     /locus_tag="ACG8E6_000586"
     CDS             7921..8991
                     /locus_tag="ACG8E6_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5061033.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9006..9674
                     /locus_tag="ACG8E6_000587"
     CDS             9006..9674
                     /locus_tag="ACG8E6_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5061034.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9694..10272
                     /locus_tag="ACG8E6_000588"
     CDS             9694..10272
                     /locus_tag="ACG8E6_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5061035.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10675..11586
                     /locus_tag="ACG8E6_000589"
     CDS             10675..11586
                     /locus_tag="ACG8E6_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS5061036.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11842..12759)
                     /gene="rnhC"
                     /locus_tag="ACG8E6_000590"
     CDS             complement(11842..12759)
                     /gene="rnhC"
                     /locus_tag="ACG8E6_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFS5061037.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            12961..13398
                     /gene="zapA"
                     /locus_tag="ACG8E6_000591"
     CDS             12961..13398
                     /gene="zapA"
                     /locus_tag="ACG8E6_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFS5061038.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13456..14004
                     /locus_tag="ACG8E6_000592"
     CDS             13456..14004
                     /locus_tag="ACG8E6_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFS5061039.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14119..16479
                     /locus_tag="ACG8E6_000593"
     CDS             14119..16479
                     /locus_tag="ACG8E6_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFS5061040.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16566..16880
                     /gene="trxA"
                     /locus_tag="ACG8E6_000594"
     CDS             16566..16880
                     /gene="trxA"
                     /locus_tag="ACG8E6_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFS5061041.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17062..18840
                     /gene="uvrC"
                     /locus_tag="ACG8E6_000595"
     CDS             17062..18840
                     /gene="uvrC"
                     /locus_tag="ACG8E6_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFS5061042.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            18985..19884
                     /locus_tag="ACG8E6_000596"
     CDS             18985..19884
                     /locus_tag="ACG8E6_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5061043.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            19877..21139
                     /locus_tag="ACG8E6_000597"
     CDS             19877..21139
                     /locus_tag="ACG8E6_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5061044.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21300..23198
                     /locus_tag="ACG8E6_000598"
     CDS             21300..23198
                     /locus_tag="ACG8E6_000598"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFS5061045.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(23497..24216)
                     /locus_tag="ACG8E6_000599"
     CDS             complement(23497..24216)
                     /locus_tag="ACG8E6_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFS5061046.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    24406..24453
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            24553..24885
                     /locus_tag="ACG8E6_000600"
     CDS             24553..24885
                     /locus_tag="ACG8E6_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061047.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            24872..26875
                     /locus_tag="ACG8E6_000601"
     CDS             24872..26875
                     /locus_tag="ACG8E6_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFS5061048.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            26894..27364
                     /locus_tag="ACG8E6_000602"
     CDS             26894..27364
                     /locus_tag="ACG8E6_000602"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFS5061049.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27385..27969
                     /locus_tag="ACG8E6_000603"
     CDS             27385..27969
                     /locus_tag="ACG8E6_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFS5061050.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            27984..28970
                     /locus_tag="ACG8E6_000604"
     CDS             27984..28970
                     /locus_tag="ACG8E6_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFS5061051.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            28960..29271
                     /locus_tag="ACG8E6_000605"
     CDS             28960..29271
                     /locus_tag="ACG8E6_000605"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFS5061052.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29293..31074
                     /locus_tag="ACG8E6_000606"
     CDS             29293..31074
                     /locus_tag="ACG8E6_000606"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFS5061053.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31067..32443
                     /locus_tag="ACG8E6_000607"
     CDS             31067..32443
                     /locus_tag="ACG8E6_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFS5061054.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32450..33085
                     /locus_tag="ACG8E6_000608"
     CDS             32450..33085
                     /locus_tag="ACG8E6_000608"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFS5061055.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33115..34470
                     /locus_tag="ACG8E6_000609"
     CDS             33115..34470
                     /locus_tag="ACG8E6_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS5061056.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKITIHSKIALTVTGTLLLGGFVVFFFTERNGETLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34662..36056)
                     /locus_tag="ACG8E6_000610"
     CDS             complement(34662..36056)
                     /locus_tag="ACG8E6_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS5061057.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQKLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQNKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36148..37119
                     /locus_tag="ACG8E6_000611"
     CDS             36148..37119
                     /locus_tag="ACG8E6_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFS5061058.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKDKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSATLTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37116..37897
                     /locus_tag="ACG8E6_000612"
                     /pseudo
     CDS             37116..37897
                     /locus_tag="ACG8E6_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            complement(38066..38437)
                     /locus_tag="ACG8E6_000613"
     CDS             complement(38066..38437)
                     /locus_tag="ACG8E6_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5061059.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TTPSYYFVIDPDGYKIEVIRG"
     gene            38571..39014
                     /locus_tag="ACG8E6_000614"
     CDS             38571..39014
                     /locus_tag="ACG8E6_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS5061060.1"
                     /translation="MDKPIFGEKKQSANYQTRYAAYIVIERDKRIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39057..39866)
                     /gene="yidA"
                     /locus_tag="ACG8E6_000615"
     CDS             complement(39057..39866)
                     /gene="yidA"
                     /locus_tag="ACG8E6_000615"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFS5061061.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(39885..41252)
                     /locus_tag="ACG8E6_000616"
     CDS             complement(39885..41252)
                     /locus_tag="ACG8E6_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5061062.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHKVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41236..42066)
                     /locus_tag="ACG8E6_000617"
     CDS             complement(41236..42066)
                     /locus_tag="ACG8E6_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFS5061063.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42229..42666
                     /locus_tag="ACG8E6_000618"
     CDS             42229..42666
                     /locus_tag="ACG8E6_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFS5061064.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42742..43362
                     /gene="rpoE"
                     /locus_tag="ACG8E6_000619"
     CDS             42742..43362
                     /gene="rpoE"
                     /locus_tag="ACG8E6_000619"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFS5061065.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     EKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            43551..44057
                     /locus_tag="ACG8E6_000620"
     CDS             43551..44057
                     /locus_tag="ACG8E6_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS5061066.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            44074..44973
                     /locus_tag="ACG8E6_000621"
     CDS             44074..44973
                     /locus_tag="ACG8E6_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFS5061067.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKARR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            45060..45945
                     /locus_tag="ACG8E6_000622"
                     /pseudo
     CDS             45060..45945
                     /locus_tag="ACG8E6_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
BASE COUNT        15178 a         7954 c         9730 g        13166 t
ORIGIN      
        1 ataaaaaagg aagaatattc atgtaaacga ttttattgtt tttttctctt ttatacaaaa
       61 agggtcataa tttcaagtaa gcggatgaat tcatgaggat tttttgcata ctgtctctac
      121 tatacttagg tcactaagaa aaaggagtga tttattatga aaaagaacat gattgggaca
      181 ttattacttt caacacttct tctaggcggc ttggcaacac cagcttttgc tgagggtcaa
      241 gcgacttcaa aaggagatat tacctttact gaaccaacaa ataccgttga gcctcttaac
      301 cctactgatc cttcaaaacc agtagagcca gcagatccgg aaaatccagc aactggccag
      361 acaggttctt taacattgga tgtagttcct gaattgccat ttggtacaca cgagattgaa
      421 agcggaacaa aaacgtatca agtagacgct tcaaaaaacg atacacctta tctccaagta
      481 tcagatcgtc gaggtgtagg tgcagatggc caagcacaag ggtggaacgt gacagtttcg
      541 gtttcagatt ttgtaaacgg ttcacaagtt ttgcaaggcg ctgaattaga ttttggaaca
      601 tccactgtta aaagtacgtc agataacgaa tccactggac caactagtca aactgtaact
      661 gggttgagta aagcaagtgc agcgacacca atatttacag cagcaaaaga tcaaggtctg
      721 ggaacatggc tttctgttta tgatccagca aatatcacac tgaaagtgcc gaaagcagca
      781 gctggaacat tcacggctga tttgacttgg aacctcgtgg ctggcccagt agcatagagt
      841 atgaaataaa ttcaacaaac tgcggtgaaa atactatagg tgaaggattt tttaccgcag
      901 ttgttgttga aaggatgtgg tggacaatga atcaaagtaa acgactgttt gtttcgtttt
      961 tttctatttt aagtctattt ttcatttttc cgtcaatcag ccaagcaaag gattcagcag
     1021 gagactttgg aatcaaaccg gtatttccag agaaccaaat agataaagca atcggttatt
     1081 ttgatttatt agtggcaccg gaacaaaatc agacactaga agtaattatc agcaattctt
     1141 cagatgaaga acgaacattt gaggtctctg tcaatccggc ggtaacgagt gatggtggaa
     1201 cgatcgatta tagtcaaaaa aacccaacgt tagatgagac attgccgttt gatgtccgtg
     1261 acgttttgct cattgcgaaa aaggaaatca atgtatcagc acatgcagaa acaactatac
     1321 ctatagaagt gaaaatccca gcaaaaagct ttaagggacg tgtattagcg ggtattcatg
     1381 tttcacctaa agaagaagct gaaacagaaa atgctacaga aggagcacaa atcaaaaatc
     1441 gtattgctta taatctcgca gtcgttttac aagaaagtca agagacgata gaaccggatc
     1501 tgaaactttt atcaggagat ttggacgagg tcaacgcaaa gccaactgtt cagcttcgtt
     1561 tccaaaatcc tcagcctagg attatcagca atttgatatt tacgagcaag atcttttatg
     1621 aaaatcagct ttatattgaa aatacgtcta atgaattttt agttgcacca aattcgaatt
     1681 ttcacttgaa tcttgattta gctggtgata aagcaaaagc cggcgattac cgcgcagaaa
     1741 tcatagcaaa atcaggcgac agtaatgaat ggcgatttac acaaaacttt acgatcaaaa
     1801 aagaaaaagc acaaaaagta aatgaaaatt ccgtttttgc agtacaagaa caatctttcc
     1861 catggcttta tgcaggattg gttgctgcag ttctagtgtt atggttgatt ttgctgtttt
     1921 tgtgggtcca caagaaaaag aaaaaaggag aaacaacatg atgaggtgat ttcgttgaaa
     1981 acatcgatct tattgggagg gattattgca agtttgctgt tcttcagctg gcaagagcca
     2041 gtccaagcag ataccaaaag ttcaggtacg attacatttt atgatctaag tccagttccg
     2101 catccaagtg gttcagtcaa tggcgcatca aaacagcaga cacagctacc gatttataaa
     2161 gaatctaatc aacatggcgt attcccggag acgaatgaag aaaagatccg ttttctttat
     2221 ttgatcattg gtatagaatt atggcttctt gcgtggttgt tgttatggag aagaaaggaa
     2281 aaggaggatg aacatgttgc tacaaaataa attttcattt tttcgctcct tcttttttac
     2341 ttcgcttttt gttttaagta tatttatctt ttcaccttcg gcagaagctg ttggagcaac
     2401 gcctcctgcc atttcttatg atccatacac gtatgaaaat atcgtacctc caaatggtgg
     2461 acgtagtgca gtcaatggcg ggaagcttgt ccaactaact ggggctaacc aattgcctaa
     2521 attgactgct acttatttga acgatggttc aaatgatcct tggaaaggtg gcacggtccg
     2581 ttttcattgg gaaagccatg tgaaactaac aaatgtactt gatttggtga atatcactgc
     2641 acaaggattt gcatttgtgg cgatcatcaa tttgccggaa catgtatcaa gcagtgatgt
     2701 attaaaagca attgactgga acaacgccta cttgtatatt gccggtgccc ccatcaaggt
     2761 tactagcggt agtttattat cagcaggaag ccataccttg cgttttggga tggggtcgtg
     2821 ggatacaaat actctattgg cagatattat tgctattatt acgcgaggcg aattcaacgt
     2881 ggacgatatc ccgcttcaat ttgatgttac agctaatgtc gcgaagatga cagaaaacgg
     2941 agataaatat gatacgagtc caaacaaaat tttgaccaag ggtaaattac aaccttctat
     3001 gtctaaaaag gccgtttttt ctgttgattt ttatgattcg aataatatta tccaagatcg
     3061 tatgttcaat tcgattttag ctccagctgc aggtagattt tatcctaaat taactggcgg
     3121 aaacttgaat ccaggagcat atgcattttc tgcaagtgca gagataaata cgtggaatag
     3181 ttacatctca acaagtgatt caacaggaaa atataataca tttgctgata ccacagaata
     3241 cgtggatgga aataaccaga accttccagg ctctaccaat agtcggacat tgacgatggg
     3301 gctgactgaa ggacgttttg atcagttgcc atttagtcgc tttgatcgcg tagtcaacta
     3361 ttttacagga aaaaacgtta cacaaggagc aatactgact catactccag caagcacaag
     3421 tggattgaat cagacagcca atatcgtgta cagtgggaaa gatgcgcaag gcggattgct
     3481 ttctcctgtt gctttaaagg tattggagca gtacaagcta gacggaacgg ttttaccaac
     3541 aaacttaaca gccgatagtc agtggggaaa gctcctttct tctgcatcgt accaagtcac
     3601 ggccaattgg tcagcaactg aactaaaaaa gggttcgatc cattacaggc tttattcgaa
     3661 aggaacccaa aaattagcga caggatggac agatcaactt ttccaaacga tccaatcaaa
     3721 taacgggggt gtaaatacag ctacttctac actacctgca ttggcttcag ggcaatatta
     3781 ttttgattat cgcttggtgg acgatgtact tactaacatg tatccgtctt ttgcgtacaa
     3841 atggcagtct gaacaagctg gaatcaccac tcttcctcaa ataacagtgg ctaattttcc
     3901 atctgtgagt gcagatagtc agctaaaaaa ccttagcaga caacctgaga caggagatcc
     3961 cctgtctgct ctatcaggag atacgattca agaaaatgtg acattcacgt taacaaaatc
     4021 tggggatgcg ataaccgaca aaaaaatcac gatctcactt cctgaaaata ctacttatat
     4081 caatggttcc ttaaaattaa atggtacaac tatcgcagat accggcattc aacaaggagt
     4141 ttctgtccca gcagatctat tgagcaaaat aggagatacc attcatctta cttacaacta
     4201 tcaattaaat actgtagata cttctgtaca atccgtcagc atactgacaa aggcagctgt
     4261 actttccagc aatattacat tagcagatgg tgctaagctt cctaacccag ttgtccaaac
     4321 atctgctaaa acaattcttg tccccaaaca agaattaaca ttagttaatg taccagatga
     4381 ttttacattt ggtaacgatc tccccaaacc gttgaagaca agttattatg aagcaaaagg
     4441 cgatttttct tttgatgtgc gggatactcg gcttccaagc acgtctcctt ggcaactgac
     4501 agggacattg acttctttat ttaagaatga tcaaggtcaa gaattaagcg gaacaaaatt
     4561 atacttcaat cattctggat caaaacaatt gattcagcaa ggtcaaaaca cgttgattta
     4621 tgagagtgat ggaacagcta aaggagaggt tctcgttgac tttcccgata ccgatggttt
     4681 actcttagag gtaaatagca gtacgaatgc tcaacctggc gcaacgtatc aaggcatggt
     4741 cacatgggaa ctgacagctg ggccaaccag ctaagtgatt agaatttgta tggatctgtg
     4801 ataactgtca cagatcctct ttttcaagga tgagaaggaa acttctgctg ttaaaatcag
     4861 aaatgcttgg aggagtggac ttggaacttt tcagtttgct tgaaaaaaat gaacaacggc
     4921 aaatcaaact tttccaactt ttattagagg aaaaaggaga gatttccgtt aataacgtac
     4981 ttacccgctt atcagttgat cgggctacct tgaaagaaga tctgttttat ttacaagcta
     5041 acttggaaaa tatcaaacaa ttggaaattg ttatccaaga agggaatatt ttattaatca
     5101 gacatggaaa tatagcagca gtcgatgttt attatcattg ctacttgcgc tattctacaa
     5161 aatatcagat acttctttat ctgttggaaa atggctcatt tgatcggcag aaacttgtca
     5221 tggaacttaa tatcagttcg gctactttgt ctcgcagagt gaaggaatta aatgattctt
     5281 taaatgagtt tcatctgaaa ataaaaagcg ggcggctgac cggtccagaa agtcaaattt
     5341 gttattttta tttccaatta ttttggtttg gacgtcctta tcttgaaaac aaaaaagaat
     5401 acagtgattt atcaggaaac acttttttag atctgctgca aaaagagttt agttttacta
     5461 ttacagaaga aggacgattg aaatttgaaa tatggctttc cattatgaag aagcgaatgc
     5521 gctgtacgaa aaaatccaaa ccagattata gcaatcttcc tgttggattg tttcaaacga
     5581 cacaattttt attggacctc catacatttt tgacccgtta cttctttcaa tatgcctttc
     5641 cttggaatca aaatgaaacg ttggtttttt atcttttcat gatcagtaac tttacattag
     5701 atacacataa ctcgcatgtc caggagtttt tgataagcga aaaatacaaa gacgacgtag
     5761 tagctcaatt gaatcgaacg tttaaacaat cgatagaaga atatttctct ttttatgttt
     5821 ttccagaaga gatttcgcaa aagattgcgt tgacagtgat gcaaaatcac gctcattttg
     5881 cctattttag aggatggatg aatatttttg gccaagaagg attaaaacag cggttaactg
     5941 aagtagaaga taaacaattc ctggttttat gtgaaagatt ggtcagacaa acatttgcta
     6001 ttgttgattt tactgaagta gacgaggaaa atgcccgctt tgaattattt gctcgctatg
     6061 catctatttt gcggacgctg atcaagcagt tagaattacg tctgcaaatt gcttatgatc
     6121 ttccttatga aaggctaatg gcggatatcg tgaaagaaat catccaggaa caattgctga
     6181 atacgatcaa atatgattta gtcgaatacg agaaagaaaa gcagtatgat atcattttga
     6241 ctagccaatt gaaagagtac ccgtcacaga agtccgctaa agtttttgta ttcactagca
     6301 acgaatataa ttatgatttc ccctatttga accaattttt aaaagaatgt tatttagaaa
     6361 aattgaattt aaggatgaat aaaacatgaa aatcgtgatc attggtgctt cccatgcagg
     6421 catcacagct gccctaaatc taagaaaact tcaaccgaaa gcagaagttt tactgatcga
     6481 tgaagatcat aaagatggac taggctatgt ttcaaatgga atcaaccttt atttaaaagg
     6541 aaagatacgt agtttagctg aagttgccca taacatgcgg actcttcaag aaagtggggc
     6601 aaaactgatc accgaatgga gagtaactga acttgatcca gacaaacatc agctcatcct
     6661 cgcatccaaa gaaggaaaaa acgagaccat aacctatgat aaattaatcg tagcaacagg
     6721 ttcctctcct gtcaccttgt ataaacaaat tgaagctgaa aatgtctaca cctataaaaa
     6781 tctagtgcaa tctaagcaag tacttgccgc tttaaaagaa gcaaaagaag tagtgatctt
     6841 cggtgctggt tatattggat tggaattagc agatgctttg agaaataaag gatacattat
     6901 tcatctagtt gattatatgc cgaatgtttt atcaagatat ttcgataagg atatgatcaa
     6961 cagttttcaa aatcagctgc aaactaagca aatcaatttt tatccaaatg aattcttgat
     7021 cgattggaaa aaatcagaag agaaagtagt atccgtacaa cttctatctc aagcaataaa
     7081 ggcagatatg gtcatttttt ctgcccaaac tcgtcctaat acgacattgt taaaagaaaa
     7141 agtggctttg tatgaggatg agactgtgat agtcaatgaa tatctgcaga caagtgatcc
     7201 ggacatctat gcgattggag atatcgtccc agtcagtttt gacaaaaata aacggcatct
     7261 ctttttacct ttagttactc gagctgtaca tatggcaaga gcagtagcgc taactttgag
     7321 tggtcagcca accgcttacg acctcagaca aaagataaca gcagcagtca ttatagacta
     7381 ctttttagga actgtagggc tgacagaaga tgaagcgcca ttcttggaac aaaataccgg
     7441 ttcgtgttca ggagaatttg atctatttcc acaatacgat gaagaaaaca aaacagtaaa
     7501 tgcaaagctg atctaccatc cggatacatt agaaataatt ggcggacagc tgataagcca
     7561 agaattctta ttatcagatt tgaacctgct tgcggatatc gtgaaacaca aaactacgat
     7621 tccacagcta gcagttgagg attttggatt tctgtctgaa cacacctctc gttttcacta
     7681 cctcaatgag ttagctttta aagtactagc taagagagca aatagcaata gagtagataa
     7741 aagactgtga gaaatatcac ggtcttttat tgtgtgggga tataataaat attaatcaat
     7801 cgagaataaa aaattcatat aatgaaatag cggaagaatt ttttgagcat aatattcgcc
     7861 aaaaatttat caagtgataa aattgttgta taaaatgatt atttcaggag gaggagttaa
     7921 atgtttttag cattaaatga aattatgcat tcaaagttac gttatgcgtt agttgcaggg
     7981 gtgatgtttt tgatcgccta cctggtattt tttttgactg ggttagctta tggcttagct
     8041 caagataatc gaacagcagt cgacaagtgg gaggcagatt cgattgtgct ttcgaaagat
     8101 gcaaattcaa atctagggat gtccatgatt actaagaaga tagcagaaga agtcgaaggt
     8161 gggaaagtgg cgtatcttgc ccagacacct ggagtcgtga ctagtaaaga cagtacagaa
     8221 gaaggaaaaa tcaatgttag cttcttcggt atcgataaaa atcaattcat catgcccaat
     8281 cttgttgaag gaaaagcttt tgataacgat gatgaggcag taggcgatat tagtttgaaa
     8341 gaagaatatg gtctagccgt aggagataca gttaagttat ctggtagtga caagacgttt
     8401 aaactaactg gctttacgga tcatgccaaa ttcaatgtct caccagttct ctatacaacc
     8461 atcaatgctt accaagagat ccgcttcgaa aaagaagata caagtgaaaa tgcacggatt
     8521 aacgcaatag ttgtcagagg taaaatcagt aatttacctg aagatttaga acagatcaaa
     8581 atttctaaat tcatcaatga attacctgga tataatgcgc aagtattgac ttttggattt
     8641 atgatcggat ttttgatcgt tattgcggct attgtgatcg ggattttcat ttatgtactg
     8701 acgatgcaaa agattaatat ctttggtgta atgaaagcac aagggatcac aggaggattt
     8761 attgcgcgtt ctgtagtggc acaaacattc attttatcat ttgttggtat tcttcttggt
     8821 ctattaggaa cagtgggaac atcccttgtt ctacctgatg cggttccatt ccaaagcaat
     8881 tggctgtttt ttggcgtcat tagcttgttg atgctggtag tagctgtgtt aggcgcacta
     8941 ttttctgttc ggacaatcgt aaaaatcgat ccgttgaagg caattggata agaaggagga
     9001 agagcatgaa agcaatagaa tttaaagaag tagaaaaaag tttccaagat ggcgaccaag
     9061 tagttaaagc attgaaagaa acaaccatga gtatcgataa aggggaattt gttgcaatca
     9121 tcggtccttc tggttcaggt aaaagtactt ttttgacttt ggcaggcgga ttgcagacgc
     9181 ctagcagtgg ctctgtattt atcaatgatc aagaattcag taatcaaaaa gaaagtgaac
     9241 gcgttaaact acgttttaaa gaaattggct ttattttgca agcttccaat ctggttcctt
     9301 ttctgacagt aaaaaagcag ttattattag tagataagat caaacatgaa catcgtcaaa
     9361 aagaagcaaa agaattattt gagcagcttg gcgtggataa gttgatcaac aagtatccag
     9421 aagaactatc tggtggagaa cggcaaaggg tcgcaatcgc tcgtgcattg tacaatgatc
     9481 cgtccattat tctagcagat gaaccaactg ctagtttgga ttcagaaaag gcacgagaag
     9541 tagtcgatat cttggcaaaa gaatcaaaag acaaaaataa agcaacgatc atggttactc
     9601 acgatacacg gctgattgac aaatgcgata gaatatttat gatggaagat ggtgtattga
     9661 aagaaaaaga ataaccaaaa agcaggtgga aaaatggtcc atcgatacaa caaagataca
     9721 cagcaaaaaa ttttgaaaga agcgactgac ttgtttatgt ccaagggata tctcggcact
     9781 tcaacaagag agatcgcaca aaaagttggg atcacccaac ccaatctcta tcattatttc
     9841 ggtgataaag aaaaactata tacggcagtt ttagaaaacc acttaaaaga tgttggaaaa
     9901 gctttgagag aaatcgttca aacaagcgaa acgggatttc aagtaacctt gacaaaaatg
     9961 gcgcagtttt tgattgagac acatctggtc gatttgtttc tgatgcttca cgatcttgaa
    10021 agcaatcttt caaaggaaac aagagatcat cttttctttc tatggaagaa aaattatcgt
    10081 gaaccttttg aagaaatctt ttcagcaaac caatctgtgc taagaaatgg gatttcaaaa
    10141 gaaatcgcag caaggcattt tttcttagtg ttagcacctt acatcactcg gtcgaaaaga
    10201 cctgtggaag aaccgctaaa tgtggcgcaa ttgatcgatt tatatttgaa tggtgtatta
    10261 ggagaagcgt aaagctgtta tatagggaga aaaatagtta ggtgaagaag caagatatga
    10321 cgaagatcgt tatatcttgt tttttttata taggtagttt ttcataaata caaatttgtt
    10381 attctctcat gattatcagg gtatattttt atttattatg tcggtgttcc aggaagtgtt
    10441 ttaatttaat aacgaagaaa ttaattgttt gtagaatgaa ggaaactatt tattgggtag
    10501 atgaatcacc acgaatagtc aaaaggagat ttcaggatat gccaaaaatg aaaaaaatca
    10561 ttttagcagt aatgttattt tctacggtta tatcttttac agcccctttt ttgcgtactt
    10621 aagctgattt taagttttga cccttagtct ggatctacga ggtgagagtc aagaatgaaa
    10681 aaaataattg gagcttttgt atttgcaatc attatcatta ttatctgttt tcaaatagat
    10741 gtttttggtc aaaaaaagat agttggacaa acggtcagca gtacgaacag tacggttaca
    10801 aagacaaagg aatcatttga tgatacagaa caagagacat taatcaaaat cggaaaaaat
    10861 gaccgtacac tatatacaga tgactcgcta actaccccac ttgcaactat aaacggcgga
    10921 gagctggcgg agtttctttc tgaaacaaaa aatgcttatc agatcaagac aaatgatggc
    10981 tatactggat atttagccct cgaagatgga accaaagtca caaagaatat acaaacaaaa
    11041 ccggaagagc tatctgatgc agtgattgta ttagatcctg ggcatggcgg aaatgatact
    11101 ggggcattaa gcaatgatga acaaacagaa gaaaaagata taacattaag tacagccata
    11161 aaagtaaaaa aggcgctaga ggatgctgga gcaacggttt atttgaccca tactacagat
    11221 gaattagtac agcttggaga tatatgcgac tacagtgaag aaaaaaaggc agatgtcttt
    11281 atcagtttac acgcagactc tacggaatat gcgaatgaag caactggaat cacgacatat
    11341 tattactatg gacaagagga aacattagca cagacaattg ctgactcctt cactgatctg
    11401 cctctggatt caagaggaat ctccactggg aactatcaag ttcttcgaga aaatcttcaa
    11461 ccttctattt taatcgagat gggttatatg aataatgata gtgatttaaa ggaattagta
    11521 acagacagtt atcagcagca gattgctgca agtttgacac aagcattgac cacttatttt
    11581 cagtaataaa taagcccagc caaacaaaaa tagctcctca tatttttcgt ttggccgggc
    11641 ttattatcgc agatgcaagc ttttgaacac cgtttaagga agaggttgtg agcctgccgt
    11701 ttaactccgc gtcacaagga gcaatttcaa aaaacagctt ctcatgtttg atgactattg
    11761 tgacttgtga ccgaggagtt ggcatgagaa caccgtttat tcggaacaag ggactgtgac
    11821 atgacttttg tcacagttcc attatttttt tagcagcttc aaagcttttt ctgtattggc
    11881 aaaatgaagt ttggcatatt tttcaagcat aggcatgccg cctttctcca atagttttgc
    11941 cgctacttgg tcagatttcg ttccagctcc tgatggaagg gtgattccag cttcagctga
    12001 tgctttgtgt aactcttcca aaaagctggc acgactgatg atagaagcag cagcaacagc
    12061 taaatgatat tgttctcctt tagtaacgaa gtacagtttt tcttgtaatt gatttttttc
    12121 taaacgcgca tattttttaa aatttgcttc cggcgtaaac tgatcgatca aaatcgcttc
    12181 tggtttttct ggctcgattt tttttagcag caaatggatc gcttgattgt gcaaagcgac
    12241 tttcatccga acggcattgt attttggttg gatctcattg tatttttctg gagataagac
    12301 aagcagttta taggggatgg ttttttttaa gatgtcagca agctggatga tttggctatc
    12361 ttttaactct tttgaatctc taacaccaag tttacgtaat tctgcaagct gatcacgttt
    12421 cacataagca gcacatactg ttagcggacc aaaatagctg ccatttccga cctcatcact
    12481 acctaaaacg gataattgac tgatattttt tggcaaagaa gaggctgttt ttgctggctt
    12541 ggcttgttta tcctgtgcct ccccccattt atcagcttct attgctgcat tcgtaccttg
    12601 aaacatcact tttccggatg tataagcggt aatggtcgtc ccgtcttttt ttgcagcaaa
    12661 gctagtataa ggaatctgtt tatttaacaa ataagagcga tagtatgctt tcatttgact
    12721 aagttggtcg tttgttgctt tgataatgac gttgctcata aaattctcct cgtttttata
    12781 cataaaacta taccgcaact cacgactttt cgttttctaa gagattgcga gacatagcat
    12841 ttttttaaag tttatcatat aatagttaag tatgtgaaaa atatttgtgg agaacggtga
    12901 aagaaccaac ttcatggtac actaaataaa actttcatca aataggagga aatgattcgt
    12961 atggcgcacg aaaaaacacg ctacaaagca gtgattgcca accaaacata tactattatc
    13021 ggacgggaaa ccaagcatca tatggatatc gtaacgaagc tgatcaatga acagttagca
    13081 gaattgaaac aattatcacc acaaatggat aatgaacaag cagcaatctt gatggctgtc
    13141 aatgcgcttt cggatcaatt gaagaaacaa gaaagaatct tggagctgga agaagaaaca
    13201 gcagaattaa agaaaaaaat gatcaaattt acagagcttg aaaatcgtgt gaaacgcatt
    13261 gaagcaatcg agaacgaagc tcgggaagta ttgaaagaaa atggtcaagc agatcatacc
    13321 attaaaaatc atgtggaagc acaacagatt ttaaacgaga aacgaaaagg acaaattaaa
    13381 caaaaatctt ctaattaaga tccttttatc aaaataagaa aaatttaaaa aagttagaag
    13441 agaaaggaac gtagcatgct gagtttgctg attttatttt tattgctaat tgcgtttttt
    13501 tctggagcac gtcgcgggtt tgctttgcaa atcatattcg cgattggata tgtattgtca
    13561 tttattgctg cccagcattt ttacaaacca ttagcaagtc atttggaatt atacattcca
    13621 tacccagctg tcaccccaac atcaaagtta gcattttttg atcaggtatt tgcttttcac
    13681 ctagatgaag ctttttatgc aggaactgct tttctgatta ttttgctaat tggctggctg
    13741 cttacccgat ttgtgggggt ttttgtccat ggattgacgt atgtgccgat cttgagacag
    13801 gtagactgga ttgcaggagg catcctgtca ctaatcatgg cttacgttac tatatttttg
    13861 attttgcagt tacttgcctt tgttccatta gatgtggtcc aaaatcaatt cggcgagcat
    13921 agtttcgctc gttttatcgt tgagagaacg ccatttctta ccaacaagat gtatgagctt
    13981 tgggtaacga atattttgaa ttaaggaaaa tagtgctgga aaatatttcg ttttttgtac
    14041 agagtagttt atttaagaag gctgcgtagc gtatgtccgg cacagctctt ttttcatagt
    14101 gaaagataag gtgataaaat gaataaccga atccttgaaa cattggaatt tgaaaaagtc
    14161 aaacagatgg tcagacaatt tgtagtgact gcacaaggaa aagaagaact ggcagaattg
    14221 gtaccagtga gtgagaaaca aacgatcaca aactggcttc aagaaactga ggatggattg
    14281 aaagtgcaac gtctgcgagg cgggatccct atacctaaac ttgaaaatat ccgtccccat
    14341 atgaaacgga tcgagatcgg agcagacttg aatggagtgg agcttgcaca agtttccaga
    14401 gtattgtcta cgacaagcga attgaatcgt ttcattgatg atctttctga cagtgaaatc
    14461 gaatttgcac gtttgtatat gtgggcagat cagttagtca cgatccctgt tttgagccgc
    14521 cgtttaaagg aagcaatcga tgaagacgga cgcgtgactg atgacgcttc tcctgagtta
    14581 aagagcataa gacagaacat tcggcgaagt gaacaagctg ttcgtgaaca gttagatggg
    14641 atcgtccgtg ggaaaaatgc aaagtattta agtgatgcga tcatcaccat gcgaaatgac
    14701 cgctacgtta tcccagtcaa acaagaatac cgcggtgtat ttggcggtgt agtccacgat
    14761 caaagtgctt caggacagac tttatttatt gaaccaaaac aagtggtgga tttgaataat
    14821 cgtttgcgtc aataccaaat cgctgaacga aatgagattc aacgtatttt aagtgaactt
    14881 tctgctgaac ttgttcccca tcgtcaagaa atcatccaca atgcttatgt gattggtaaa
    14941 atggatctga tgaatgcaaa agcacgattt ggtaaagaag taaaagccat cgtgccggga
    15001 atcagtgaag acaatcatgt cgtcttgaaa caagcaagac atccactgat cgatcaggaa
    15061 aaagtagttt ctaatgacat cacaatcgga aaagactatc aagccatcgt cattacagga
    15121 cctaacacag gtgggaaaac gatcacgttg aagactttag gtctgttaca actgatgggg
    15181 caagcaggat taccgattcc tgcaggtgaa gaaagtcaaa tcggtatttt tgaggaagta
    15241 tttgcggata taggagacga acagtcgatt gagcaaagtc tatcgacctt ttcttctcat
    15301 atgacgaaca ctgtagatat tttatcaaaa gtcaatgaaa aaagtctggt attatttgac
    15361 gagctcggtg caggaacaga tccgcaagaa ggtgctgctc tagcaatcgc aatcttagat
    15421 gatcttggta aaaagtctgc ttatgtgatg gcaacaacgc attatcctga attgaaagtt
    15481 tatggataca atcgggcaaa tacgattaat gccagcatgg agttcgatgt ggatacacta
    15541 agtccaacgt atcgtttgct gattggcgtt cctggacgaa gcaatgcgtt tgaaatctct
    15601 agccgattag gtcttgatac agaagttatt gatgaagcga agcagttgat gaatgatgag
    15661 agccaagatt taaacgaaat gatcacagat ttagagaatc gccggaaaat ggcagagacc
    15721 gaatatctgg aaatgcgtca ttttgtttct gaggcacaag aacttcacga tgatttgaaa
    15781 gaagcttaca gctatttctt tgaagaacgt gaaaaagaaa tggaaaaagc aaagaaaaaa
    15841 gcaaacgaag tcgtgtcaga ggcggaagaa aaagcagaaa aaatcattgc tgatattcgt
    15901 aaaatgcaac agcagattgg gcaaggaaat gtgaaagagc atcagttgat cgatgcaaag
    15961 acccaattag caaatctaca tcaagaagaa actttgaaga aaaataaagt attgaagaag
    16021 gcgaaagaac aaaaaacgtt gaaaccggga gacgaagtgt tggttacgac ttacggccaa
    16081 agagggacgc ttttacgtaa aaacgggaat cagtggcaag tagaaatcgg tatattaaaa
    16141 atgaatgtgt ccgaagacga gctgacgcct gttgctcctc aaaaagagcc gacacaacga
    16201 gttatccatg ctgtacgatc tgaatctagt agtcacgtgc cgaatcaatt ggaccttcga
    16261 ggaaaacgat acgaagaagc gctaagtgag gtagatcaat atttggattc tgccattcta
    16321 gctggttatc ctcaagtgac tattgtccac ggaaaaggaa caggcgcact tcgcaaggga
    16381 attactgatt atcttaaaaa ccatcggagc gtcaaaagct ttgagtttgc accggcaaac
    16441 caaggcggaa atggtgcgac aattgttaaa tttaagtaaa gcttactaaa ggtaagcaga
    16501 tggttcgcta tccaagtata gaaatgctat actaatcata gattttaaaa atggaggtct
    16561 ttgatatgtc acaagtaatt acagataaga catttaatga agaaacagat aaaggacttg
    16621 tattgattga tttttgggca acttggtgtg gtccatgtcg tatgcaagca ccgattttgg
    16681 atcagttgga acaagaatat gatgaagaag aattcagaat tgctaaaatg gatgtggatg
    16741 aaaatccaga aactcctcaa caattcggta tcatgagcat tccaacatta atgttgaaaa
    16801 aagatggtca agtagtagaa aaagctgttg gtgttcattc aaaagaacaa ctacgtcaaa
    16861 tgatcgatca atatttataa aaacttacag caaaaataag ctgagggtgg gactgaatcc
    16921 agttccgtcc tcggtttttt tgcggttgag ttaaggttat tcaaattaat attttttgag
    16981 tataattacg ttgtcaggct ggattgtttt tttgacagac atgctataat aactttaatt
    17041 gaatatgaag gggagtacca gatgaacgaa cgaatcaaaa acaaattagc tttacttcct
    17101 gatcagcccg gctgttactt gatgaaagat aaaaatggaa cgatcattta tgtcggcaaa
    17161 gccaaaatat taaaaaatcg tgtccgctca tattttactg gcagccataa tacgaaaacg
    17221 gaacggcttg ttagtgaaat cgaagacttt gaatacatcg ttacagaatc gaatatcgaa
    17281 gcattgctct tagagatcaa tctgataaag aaaaatgatc ctaaatacaa cattatgtta
    17341 aaggatgata aaacctatcc tttccttaag ataacgaatg aaaaatatcc ccgtttggtg
    17401 attactcgta aagtactaaa agacaaagca ctgtattttg gaccatatcc cgatgtcggt
    17461 gcggcgaatg agacaaagaa gatcctcgat cgattatttc ctctgagaaa atgcaaacct
    17521 agtcagacga aggagccttg tctttactat catttgggac aatgtctgtg tccttattat
    17581 ttcgacgtag atccgaaagt ctatacgaaa atcgttgatg aagtgaaaaa gtttttaaac
    17641 ggaggacacg aaaaaatcga gtcagagatc catcagaaaa tggaaaaagc tgctgaaaac
    17701 atggagtttg aaaaagctgc agaataccga gaccagatcc gtgccattga gacaatcatg
    17761 actcgccaaa aaatgacaaa tactgattta ctggatcgag atgtatttgg ttatgcgatt
    17821 gacaaaggat ggatgtgtgt ccaagtgttt ttcgtaagac aaggaaagct aattgaacga
    17881 gatgtttcaa tgtttccttt ctataacgaa gcagaagaag atttcttgac ttacctcggt
    17941 cagttttatc aggaaaatga gcatttcatt cctaaagaag tcttgattcc tgatacgatc
    18001 gacaagccca gtgtagaagc tttgttagct acgaaagtgc tgcaaccaaa acgaggagaa
    18061 aagaaaaaat tagttgaatt ggcgaataag aatgcctcag ttgctttgaa tgaacggttt
    18121 gatctgattg ccagaaaaca ggaacggacg atcggagcag ttgaacgttt aggaaacgcg
    18181 atgaatatcc cgaccccaat acgtatcgag tcttttgata actctaatat catggggacg
    18241 aatcctgtgg ctgcgatggt cgtattcatt gatggaaaac cggcaagaaa agaatatcgg
    18301 aaatataaaa taaaaacagt caacggaccg gatgattatg catcaatgag agaagtcatc
    18361 tatcgcagat attcacgagt gattcgcgaa gggttgcctt tacctgattt gatcttgatc
    18421 gatggaggaa aaggacaagt agatgtggca aaagatgtct tagaaaatca attaggaatc
    18481 gatattccta ttgctggcat ggcaaaaaac gataaacata aaacaaatga gttgctattc
    18541 ggtatagatt tggctgtagt accgcttgag cgaaattcac cagagttttt cttactccag
    18601 cgaatccaag atgaagtcca tcgattcgca atcactttcc atcggcagct acgcagtaaa
    18661 aacagttttg cttcccgatt agatgaaatt gaagggttag gaccaaaacg aaaaaaaatg
    18721 ctgcttaaag agttcaaatc gttgaaaaat atcacagcag ccacgacaga agaattacaa
    18781 gaaatcggac tgccgaaaaa tgtcgcacaa aacgtatttg ataaattaca tcaggaatga
    18841 ggaaagggca ctttacaaag aaagtaaagt gtcctatcat tttatgtaca acaaaaagag
    18901 cgtttacgtt ttgttggtaa ataataaatc gcttacgtct ggtgtctaag gatatatgtg
    18961 atttcttgaa gaaggagtga agaaatgtta gaagtcaagg atcttgttaa aacttttggg
    19021 aactacacag ctgtcgatca tgtctctttc caaatccccg atggaaaaat catggggcta
    19081 attggccaaa acggggctgg gaagacaacg acttttcgtt tgatcttgga ttttttgaca
    19141 caagaccaag gagaagtgtt atggaatggc catcatcttg gggagaaaga ttatgatgtc
    19201 atcggttatc ttccggagga acggggactt tatccgaaag taacgattca agaccaactg
    19261 ctttattttg cagaacttag agggaaatca agaaaagaga ttgaaccaaa aatcgatttt
    19321 tggatggaaa aatttcaggt aaaagggaaa aagacggata aagtaaaatc tttgtctaaa
    19381 ggaaatcagc aaaaagtcca gctgatcgcc acgttaatcc atgaacctaa attgatcatt
    19441 ttggatgagc cattcagcgg gttagatccg gtaaatgctg aattgctaaa agatggaata
    19501 ttggaattga aaaaacatgg ttcatgtgtc attttttcta gccacaatat ggataatgta
    19561 gaaaaaatct gcgatcattt gatcatgttg cgaaacggaa aaatggtttt gaatggaaaa
    19621 gtacatgaga ttcgtgaatc atttggccgt acgaagttat tcttggaatc tggtttaagc
    19681 caacaggaag tagaagaaat cgaaggcgtg aaaaaagtga tccttcatga agacaatacg
    19741 ttagagatta ccttagaaga tcctgaagct gggaaagaaa tctttactcg tgcgactcag
    19801 ttcggctaca ttcctatgtt taaccagcaa ccgccaactt tggaagagat tttcaaattg
    19861 aaagcaggtg aaatcgatgc gtaagttttg gattattacc aaagatgtct atttaaaaaa
    19921 tgttaagtcg atttctttct tgatcatgat attagtccct tttatcatgc tgggtatcat
    19981 ttatgtagca ggaaactttg ctcaacaaaa tagtgatgtc aataaaatcg gcgtattggc
    20041 tgataatgag caaatcagtc agcaaatgag ccaagtcaaa acagatgatt ttgagttcaa
    20101 agcagttgat tctgaagaaa aagcacaaaa agagctttcg gaagaaaaaa tcgatgcgta
    20161 tttagtagtc tcaactgaga atgataagat aaatgggatg ctttatagcg aaaattcatt
    20221 aggacaaagt acacaactac tgatacaaca acaactgact ggcttgcaat cgatgcttcg
    20281 agcaagtcaa ttaggcattt caccagaaga agtagctagc ttgagccaac aagcaagttt
    20341 gactcgacaa aaagtcaatt ttgattcaaa aggaaaaatg acagttggag aagataactc
    20401 gacagtccaa tatgtcgtaa gttatgtagc aacgattgtc ttgttcatta ttattttgac
    20461 gtatgcgcag atcattgcac aagagatcgc ttctgaaaaa ggcacgcgga tcatggaagt
    20521 gatcctgtcc agtacgactg cacaaaagca tttttacgga aaactaacag gtgttttact
    20581 tgttgcatta acgcaaatgg cattatacgg tattattttt ggtataggat acagtcaatt
    20641 caaagatatg gaaatggtca aaaatatatt agacggtatc tcattggata acttgattgg
    20701 accattcttg tggtattctc ttctattcat gatctttggc attttgattt tttctgtttt
    20761 agcagcttta tgtggctctt tggtcaacaa agcagaagat acagcaaaag caattttgcc
    20821 agtgacttat ctatctctag gcggatatat gcttggattg atactaggag cttcagatcc
    20881 aaacaatatc gttattcgga tcacttcgta tattccattc ttatcttcat atattatgcc
    20941 ggttcgctta gcaaatgaga cagtcggtat gggtggagca ttgatttcct tgttggtact
    21001 tgtagccgta acattcttat tgatgatctt gtctgcaaac atgtacaaat cgaatgtact
    21061 cgtgtatagc gaaggcggat tatggagttc attgaaacaa tcgatttcaa ttatgagaaa
    21121 tgaacgaaaa aaagcataaa atatagaaaa atatagaagg aagctggaca tttattgttc
    21181 ccagcttctt ttttgtatag acctatttct atttttttat tatattggat atatattcat
    21241 tgaaaataga gcataacaac tttataatgt tgagtatacc aatttttgga ggcgttttaa
    21301 tggaccgcta tttagattta ctttcagaga agttccctac aacggaagat gtcatcacgg
    21361 aaatcatcaa tctcgaagca attttgcagc ttccaaaagg aacagagtta tttttgagcg
    21421 atatacatgg cgaatttcct gcttttgatc atattctgcg tatcggttca ggaaatctta
    21481 aagaaaagat caaagacctt ttctctgaac aaatgacgga aacggagatc agccaattca
    21541 ctatttttac ggcttatcca gaatatgtgt tgacgactga ttggtacaaa aaacaagata
    21601 aatcccagct gatccatcgt ttgatcgatc ttttacggtt tacgacagtc aaatacactc
    21661 gatcaaaagt tcgaaaagga ttgccgaaag aatatagcta tatcattgag gaactcttgt
    21721 atatcgatga tcgtatcaac gggaaaaaag actatgtaaa aaaaatcatt gaacaacttg
    21781 tcttgatgaa ggaagaagaa aaatttttaa caaaacttgc gcagacgatc caaaaatctg
    21841 tcatcgatca tttgcatatc gtgggtgata tttttgatcg aggaactcaa gcagccaaag
    21901 taatggatcg aatcatggat ttttcctctg tagatatcca gtgggggaat catgatattt
    21961 tgtggattgg tgcgtattgc ggctctgagg catgtttatt gacgctttta cggattgccg
    22021 cacgctacaa ctatttattt gaattagaga aagaatatgg attaaattta cgtccattat
    22081 tttcttttgc ccatcaaacc tatcagaaga atccagtatt tacccctaaa aacaggaaag
    22141 atctatctga aagagagcaa gaagaattag aaaagatcca tcaagcactt tctatcctcc
    22201 aattcaaatc agaacatcag ttgttgaatc gacgacctga atttaaaatg gacgatcgta
    22261 aattgttgga aaaaatcaac tatgaggaat caacgatcgt tttagaaggt cagctctatg
    22321 agttgaaagg gacttgtttt caaacgatcc agccagatga tcctaaaaaa ttcttggaag
    22381 aggaacaaaa agtgatcgat agtttgatgt attcattcca gcactcgtta agaatgcaaa
    22441 aacacatgac gtttttgatc gaaaaagggg gaatgtatct cacgaacaat cagcatgtac
    22501 tgttccatgg ctgtatacct gtagatgaaa aggggcagct cttagcattt gaattagacc
    22561 aatcttatcg cggaaaagaa ctgctgggat tttttgaaaa acagataact gaaagcgcaa
    22621 aagatttgac tgcaaatgaa gatttggcga ccgatttgat ttggtatgcg tggagcgggc
    22681 cattttcgcc tttattcggc aagagtaaaa tggcaacttt tgagcgctat tttctcacag
    22741 agcctcatac gcacgtcgaa aaaagcaatc cctattatca cttgcgagat gaagagtggt
    22801 tcagtgaaaa aattttggca gaattcgatg caaaagaaga aggttcagca atcatcaatg
    22861 gacacacgcc agtaaaagta aaaaaaggag agtcaccgat caaagcaaac ggaaaagcat
    22921 ttgttattga cggaggtcta tccaaagctt atcaaaaaac aaccgggatt gctggctact
    22981 cattactatg caattcttac ggtttccaaa tcgtgacaca ttatccgttc ttgcctattg
    23041 aacaacaatt tgcaaaaaaa tctgatcaaa cgaatgtaaa aaaagtgatc gagcagcagc
    23101 tcccacgcaa acttaatcgg aatacgacaa aaggaatgga attacagcaa cagattactc
    23161 aactccaaag gctgttggat tatcgaaagg acgattgaga aaaatgctag ccaacattgc
    23221 tcaatagact aaaaagcgac tgcaacaaaa ggcgtgtcgc agtctccttc ggaattagct
    23281 caaaaaacgg taaaatatta gaagctattt ttctgtttat ttctctaata ctcaggtcag
    23341 ggcgctgatt tcacgacctc ttccttaaac ggtgttctcc tgccaactcc tcggtcacaa
    23401 gtcacaatat ccaacaaaca tgagaagctg ttttttgaaa ttgctccttg tgactcagag
    23461 ttgaacggca ggctcacgac ctaatcagaa aaacgattaa aattcattca caaagaaatc
    23521 gaataaacgc tgttcagatg aatctacacg atgagaaagt tccggatgcc attggacacc
    23581 aaggattttg tgcatattat ctttactttg gactgcttca atgagtccat cgttggaaaa
    23641 ggcgatggct tttaacaaag gagaaagatc tttgactgcc tgatgatgat aggaattcac
    23701 aaagtatgaa tcagggagaa ggtcacttaa taaactgtcc gaaacgattt tgacttcgtg
    23761 agttgcaaac tgcggggcag tgggctgttg ttcgtgtttt acggaccatt ttggatataa
    23821 tgaaagatct tgataaagtg ttccctccaa cacgacattc aatagttgaa gtcctcggca
    23881 gactgcaaaa atcggttttc tttgtttcaa tgcttccaag atcaatgcct gttcgaattg
    23941 gtctcgttga atatttgttt ccgtcagttt tggatgaggg tcttccatgt aaagttgcgg
    24001 actaacatcc tgaccgcctg caagaatgag tttgtcgatt ttctcaatta gttgaggcgc
    24061 tgaatctgga gacatgactg gcagaataag aggtaatcct tgagcatcta aaactgcatc
    24121 cacaaatccc tgcggtgtat agcttacttg atttccttgg aaagtatctg tcgctcgaat
    24181 cagttgattc ccggcaatgc cgatgattgg tttcattttt attcccccta aagtagacta
    24241 attgtaacaa ggatttaaaa aaatttaaac tatgtaagac ttgcccctat acttatctgt
    24301 tcttgctgtg ttactatctg taaagagaat tttcgtgtga aattcatgac gatagttgaa
    24361 aatattgctt aatttgttat aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct
    24421 tcaaggatct agttcttcaa ggggtgagaa ggacatttta ttgaaggggc ctgcctatac
    24481 taagttatct ttagaggtac atactttcaa tgaaaaaaga aagcatgtac ctctttttta
    24541 aatcgaaagg aagtgaagca agtgttagaa gctttggaac aaatccgaaa agcagaagaa
    24601 gaaaatgaga agcgtgctcg aaccctacgg cagcagttga agacctatgc agaagaaaaa
    24661 gagcaagaat tggagataat ccaaacgaac agtcggcaac agctgaaaca gttggtagaa
    24721 gaaaaagaac aattcgaaaa gcaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa
    24781 gcagacaagg tgcaagcaac tttgaaagac caatatcaaa agcatcagag tcaagcaatc
    24841 ggtgcaatca ttgaaagggt gaaagaaact tatggccgtc actaagatgg aaaaagtgac
    24901 cctcatttca gataaaagaa atcaggagag gattttacaa gcagtacaag gaatccaaaa
    24961 tgttgaaatc cgggatctgt atcaaaatag aacaaacaac cagtgggtag aaaaatattt
    25021 tccagatacc acgattattg ataaagaagc aaaattaagc gctttaggca atcggctgaa
    25081 agacatccaa gaagctatcc aattcataca acaccacggc gatagccgtc aaaagaaact
    25141 ccatttgaaa cgtcaagaac tttccttaca agacttggaa gcaaattatt ccgaagaaaa
    25201 tttcatgaat aaattagaag aggtccttga tttaaaagaa aagtgggaac agctaactga
    25261 aaaacgtgat cgtttagaag aagaggaaga atggcttctt cattggcaac agctggatat
    25321 tgctccaagt atgtatgcaa gtaagacgac catttttgag atggggactg tgagtgcagc
    25381 aaattttgaa agtttcaagg aagaactaag taagcttgat gacgtttata tggaagaaat
    25441 agattatacg ccaaaacaag tttatctttc gtatgttgct ttaaaaaaat cagttgctgc
    25501 tatagaagaa attgctggga aatacgggtt caataaagaa acgtatcctt atgaacaaaa
    25561 tcctaaagaa aaattgcaag aaaccaaaca gcaattatcc attgttcacg agcagcaaaa
    25621 aaagataacg acagcgttag gtcagtgcag cggctatatc caagaatttg aatgggtaga
    25681 agaagtcaca cttgccgttg cagaacggga aaaagtaaaa gaacggttta tccatgccac
    25741 ttatctgatc gttttgcaag gttgggtaga tgcagaagaa aaacagaatt tgatgcatgt
    25801 gttaaacgaa actgtatcag aagaagatat atacgttagt ttcgaaacac caaactcaga
    25861 agagattcag caggaagtcc ctactaaatt gaaaaaccat ccgcttatcg aaccttttga
    25921 attgctgact gaaatgtaca gtctgccaaa atatgaagaa gtagatccaa cgccttggat
    25981 gacaccattt tatcttgtct tcttcgggat gatggtcgca gatgttggtt acggattgct
    26041 gatgctcatt gggacaatac tagctcaaaa actatttgtt ttaccccgcg ggatgaaacg
    26101 tttcgtaaaa ttctttgaga ttctctcggt accatcgatc atctggggat tgatctacag
    26161 ttcatttttc ggacaggcat taccaaaaga actatttggt atccgcttgc ctttcccaat
    26221 cctatctacg acggatgacg tcaatacgat tttgatttta tccgtgattt tcggtctgat
    26281 acagatcttg gttggattat tcgtggcagc aaaggaaaat atcaggagga aagattactt
    26341 gagtgctgtc agtgatggtt ttgcctggca agggatcctg attgggatcg ttattgcatt
    26401 gattggtgtc atgttgataa aaaatacagc atttgtttat cttggcgcgg ggctatcgat
    26461 cattgctgct ttatgtatct taatcgtgcc gattatccaa tcgccgtcta aagtcaaggg
    26521 tgctgccaaa ggagcgtata atctttacgg attgactggt tatatcggtg atttagtcag
    26581 ttatacacgt ttgatggcac taggtatttc aggcggaagt attggcgcgg cattcaatat
    26641 gctagttgct ttcatgccgc cagccgctcg attcagtgta gggatccttt tgatcatcgc
    26701 tttacacgca ctgaatatgt ttttgacact gttaagtgcc tatgtccatg gtgcacgatt
    26761 gcagtatgtc gaattttttg gaaaatttta tacaggaggc ggacgtgcct ttgacccgct
    26821 gaaaacggcg gaaaaatacg tcaacatcaa tcataagaaa aaaaacaata aataattgga
    26881 ggatttttat acaatgatgg attacttaat tagtcaaaat ggtggaatgg tatttgcagt
    26941 attagcgatg gcaacagcga cgattttttc agggattggt tcagctaaag gtgttggaat
    27001 gacaggtgag gcagcagcag cgttaacaac aagtcaaccc gaaaaattcg gacaggcttt
    27061 gattctgcaa ttgcttccag gtacgcaagg gttgtatgga ttcgttatcg cctttttgat
    27121 ttttatcaac ttaggaaatg atatgtcagt tgtacaagga ttgaactttc taggcgcttc
    27181 tctgccgatt gcttttacag gtttgttttc aggtatcgcc caagggaaag ttgcagcagc
    27241 aggtatccaa attttagcga aaaaacctga acatgctaca aaaggaatca tttttgctgc
    27301 gatggttgaa acatatgcga ttttaggttt cgttatttcc ttcctattag tattgaatgc
    27361 ctaagcataa aggaggaaaa gcagatgaac gccattgaaa aaatcatctc tcagatgaat
    27421 gaagcagcag agcaagaaag agcggcgtta gagcaagaag agcgaatgaa gatcgatcaa
    27481 aactttgaac aaaaacgtac gcaaatcgaa acagaacatc aaaaacaaaa agaaaaacaa
    27541 atcgaattac ttgaaaaaaa ataccgtcag cttaggaatc gccaacaagt agaagtacga
    27601 caagaaaatt tgaatgcgaa gcaggaattt ttacgacgat tgtttgcaga tgcaatagct
    27661 gaaatggaga attgggatga atctgaacaa atccagttta tcaaaaattc attgtattca
    27721 ttacctttga caggcaaagt aacattcatt gctggagaaa aatcagcagc ttatctttct
    27781 cagacattgc tggatgaatg gaacaatgaa cttcccttta tgatggtttt aagtgatgaa
    27841 actgttgctg atcaagcggg ttttctgata aacgatcaag gagttcagta taattttctt
    27901 ttcagcagcc ttgtacaaga tatccaagga acgatgagtt ttgagattgc caatcaatta
    27961 ttcgaataag gagggatcag tgtatggaat accacgaact aaatcccttg attcgcggaa
    28021 gagagcttga acttttgtcc aatgagacat ttgaacggat gattcaaaca aattctgtcg
    28081 aagcacttgg tgaaatattg aaatccacga tttacagtcc atatatttat gacggatatg
    28141 aatatgactt cgaacagaat cttttaaagg agcagagccg tctatttcgc tggttaaagg
    28201 aatcagcacc ggagcctgaa atcgtttgga tctatacgat gcgctatact ttccataatc
    28261 tgaaggtttt gacaaaagcc gagatgacaa atcaaaatct ggaccatttg tatatcgacg
    28321 atggatttta ttcattagaa accttgaagg atgccattca tacgcaagtt tcaaatgaac
    28381 tccctgaaag cattatggac agtatacgag aagtacgtga ttattttgaa gagtctacca
    28441 ttcttcaggg aattgacgtg atttatgatc gacagttttt gacagagcag cgacggcttg
    28501 ccgataagct aggatatcaa gaattgctga cagaagtcat tgcttttatt gacttgacca
    28561 atatcacaac gatggctcga ggaatattgc agaaaagaac atatgggttc atgacgacgg
    28621 tactttccag ctcgggaagt atcgaaaaag aagtattttt gaactttgta gataaagata
    28681 tggaagatta cacgaatttt ctattagaga cagattatgc tgattttctg cgccctgtca
    28741 tccgtgaggg aaatcttgat ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt
    28801 cattgtatca agaagcacaa acccaggctt tcggaccact gccactacta gcctttttga
    28861 acgcaaaaga ggtagaaagc aaaaaccttc gtttactggt tatcggcaaa agaagccatt
    28921 tcgaaccaga aacaatcaga aagcgggtga gacaagtcta tgacgcataa gatcggtgta
    28981 gttggcgaca aagattctgt tttgccattc aaattgtttg gctttgatgt tcgttatggt
    29041 acaaccaagc aaaccgttcg tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg
    29101 atttatgtca cagagcaatg tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa
    29161 caaatgatac ctgctatcgt attgattccc aatcatcaag gatcattagg gatagggctg
    29221 gaagaaattc aaaaaaacgt agaaaaagca gtaggtcaaa atatcttata gtatgaaaag
    29281 gagatagaaa ttttgcaaat tggaaaaatc ataaaagtct ccggacctct tgtaatggct
    29341 gagaacatgt ctgatgccag tatccaagac atgtgtctag tgggagatct aggagttatc
    29401 ggagaaatta tcgagatgcg tggcgatgtg gcgtctattc aagtatatga agagacttct
    29461 ggaattggtc caggcgaacc ggttcgctct actggtgaag ctttatctgt tgaattagga
    29521 ccagggatca tttcacaaat gtttgacgga atccaacggc cattagatac ctttatggaa
    29581 atcacacaaa gtaatttctt agggcgcgga gttcaattgc ccgcattgga ccatgagaaa
    29641 aaatggtggt tcgaaccaac agtagaagcc ggagaaacgg tgagtgctgg agatgttatc
    29701 gggatcgtag aagaaacaaa agttataaaa cataaaatca tggtgccaaa tgggataaaa
    29761 ggtacgatca aaacaatcga aaaaggttca ttcactattg atgaaacagt atgtgtcatt
    29821 gaaaccgaaa atggtgacaa aaatttgacg atgcttcaaa aatggccagt aagacgtagt
    29881 cggccaatca aagaaaaatt aaacccggat gcacccatga tcactggaca gcgagtgatt
    29941 gatacattct tcccagtgac aaaaggagga gcagcagctg tacctggtcc ttttggtgca
    30001 gggaaaacag tcgttcagca tcaaattgca aaatgggccg atgtggacat ggttgtctat
    30061 gtgggatgtg gcgagcgtgg aaatgagatg acggatgttt taaatgaatt ccctgaattg
    30121 attgacccta atacaggaga atcgttgatg gaacgtacgg tattgattgc taatacatca
    30181 aacatgccag tagcagctcg agaagcttct atctatacgg gaatcacaat tgcggaatat
    30241 ttccgtgaca tgggttatga cgttgctatc atggcagact ccacttcacg atgggcagaa
    30301 gcacttcgtg agatgagcgg acgtttggaa gaaatgcctg gtgatgaagg ttatccagct
    30361 tatcttggtt cacggcttgc ggaatactat gaacgatctg gaagagtcat tgcattagga
    30421 tcagaaggtc gcgaaggaag tataaccgca atcagtgctg tatcgccgtc tggaggggat
    30481 atttcggaac cagtcacaca aaatacattg cgggtcgtaa aagtattctg gggtcttgat
    30541 tccagtttag cccaaaaaag acattttcct tcgatcgatt ggatacaaag ctattctctt
    30601 tattcaacag aagtagggaa gtatatggat catatccttc aagctgactg ggcatccatg
    30661 gtcaccgagg gcatgcgtat tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg
    30721 gtagggattg attccctttc agataatgac cgtctgacat tagaagtggc aaaatccatt
    30781 cgtgaagatt atttgcagca aaatgctttt gatgatgtcg atacattcac ttcaagagag
    30841 aaacaattca aaatgttgaa gatgatcttg acctttggtg aagaaggacg ccgtgcgttg
    30901 tcgttaggtg cttatttaag tgagatcatg gacggtaccg tatcgattcg tgaaaggatc
    30961 ggacgaagca aatatatccc ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa
    31021 atcaagcaaa cgatgcaaac gatcgtttca gaaggaggca tgagcaatga ttaaagaata
    31081 tcgtacgatc ggtgaagtcg tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa
    31141 atatgaagaa ttaatcgaag tccgtatgca aaatggcgag attcgtcgtg gacaagtatt
    31201 ggaagtccaa gaagataaag caatggtcca aatattcgaa ggaacaagtg gtatcaattt
    31261 acgtgactct tctgtacgat ttttaggcca tcctttagaa ctaggtgtat cggaagacat
    31321 gatcggtcga gtattcgatg gtttgggacg accgaaagat aacggaccag agatcctgcc
    31381 tgaaaagcat ttggatatta acggagaagt aatcaatcca gtagctcggg attatccaga
    31441 tgagtttatc caaacaggta tctctgcaat cgatcatttg aatacattag ttcgtgggca
    31501 aaaattacca gtattttctg gctctggtct tcctcataaa gaattagctg ctcagatcgc
    31561 gcgacaagca acggtactcg attcatcaga tgatttcgcc gtagtctttg ctgctattgg
    31621 aatcactttt gaagaagcag aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga
    31681 ccgttctgtc atgtttatga atttagcaaa cgaccctgca atcgagcgta tcgctactcc
    31741 tcgtatggct ttgactgctg cagaatatct tgcttatgaa aagggaatgc atgttttggt
    31801 tatcatgaca gacatgacca actatgcaga ggcattgcga gagatttctg cagcacggcg
    31861 agaagttcca ggaagaagag gatatcctgg gtatctttac acgaacttag ctactttatt
    31921 cgaacgagca ggaagaatcc gcggactaaa aggatcggtt actcaaattc cgattctaac
    31981 aatgccagaa gacgataaaa ctcatccaat ccctgacttg actggttata ttacagaagg
    32041 acagatcatc ttgactagag agctttacaa gagtgggatt cagccaccaa ttgatgtact
    32101 cccgtctctt tcgcggttaa aagataaggg aactggaccc ggaaagacaa gagaagacca
    32161 tgcagctacg atgaatcagc tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt
    32221 ggccgtagtt ttaggtgaat ctgctttatc tgatgtagac aagatctatg cgaagtttgc
    32281 tgagcggttt gaaaaagaat atgtcaatca aggattctac acgaaccgtt cgattactga
    32341 aacacttgat ctagggtggg aactgctatc gatgttgcca agaaccgaat taaaacgaat
    32401 caaagacgac atgctggaca aatatctgcc agaagggaag tgagttctga tggcacgatt
    32461 gaatgtgaat ccgacccgca tggaattgac tcgattgaaa aaacagttga caactgccac
    32521 aagagggcat aaattattaa aagataagca agatgaactg atgcgtcaat tcatcctgtt
    32581 gattcgaaag aacaaccagc tgcgtcaaga aatggaagag tctatgacag aagcaatgtc
    32641 tgattttgta ttggcaaatg cttcgatcac ggagcctttc atcgaagaat tatttgtttt
    32701 gccggcggaa aacgttgatt tgtctatcgt agacaaaaat atcatgagtg taaaagtacc
    32761 tgtgatgaat tttgaatatg atgaacagct gacagacaca cctttggaat acgggtattt
    32821 gaattccagt gccgaactgg atcggtcaat cgatcggttt actaccctgc ttccaaaact
    32881 tttggaactg acagaaatcg aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac
    32941 acgtcggcga gtcaatgcat tagaatatat gacgatccct caattagaag aaacgatcta
    33001 ttatattcgt atgaagctgg aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt
    33061 aaaaaatatg ggtaccactg actaagtggt atcctttttt aaaagaggaa aacgatgatt
    33121 aaacaaagca aacaaataaa gaaacgcttg ccggctgttc agcttctcgc attaggattt
    33181 tttctcctga ttttagttgg cggcggccta cttaccctac cattttttag taatagtggg
    33241 catgggacaa attttatcga tgcgcttttc actgcaactt ctgctgtctg tgtgacggga
    33301 ttgacaacat tgaatacagc agagcattgg aacagtgctg gtcaattttt gatcatgctt
    33361 ttgattgaga tcggcggtct tggttttatg atggttccga ttctatgctt tgcattgata
    33421 aagaagaaag tcagtctcag cacacgtatt atattaaaag aagcgctgaa tctagaagaa
    33481 gtatcaggtg tgatgaagct catgctttac atcctgaaat tggcctttgt gatccaactg
    33541 attggtacgg ttgctttatc ttttgtattc gtgcctgaat ttggctggtt gaaaggtatc
    33601 tggtacagtt tgttccatgc aatctctagt ttttgtaatg cagggtttga tttgttagga
    33661 gacagtttag ccaatcacca gcaaaatgtt tatttgatca tggtggtatc tgctttgatc
    33721 atttctggtg ggttaggctt catcgtatgg cgtgatttac tggaatatca tcagaaaaag
    33781 aagataacga ttcattcaaa aatcgcatta accgttacag gtaccctttt attaggcgga
    33841 ttcgttgtgt tctttttcac agagcgcaat ggggaaacgc tagtggaagg gaatatgttt
    33901 caacgattgg caaacacctt ttttatgagt gtgacaccta gaacggctgg atattattca
    33961 gttgattacc tccatatgag ccatgcaggt ctgattttaa cgatgttttt gatgtacatc
    34021 ggtggaacat ctgggtcgac ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg
    34081 atccagatgc gttcgatgct taaaggacgt acgagagcgg aagcatttgg ccgtaccatc
    34141 cgacaaggcg cagtgttgcg ggcacttact ttattttttg ttacgttgac gttgtgtgta
    34201 gtcgccatca tgattttatc ggtgactgaa acgataccta aaacttctgg catcgaatat
    34261 attgcttttg aggtcttctc tgcgtttggt acagtaggtc ttacgatggg attgacgcca
    34321 gacttgactg caatagggaa attgatcatt atagcgctta tgtacattgg acgagttggt
    34381 gtattgactg tggtcttctc tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa
    34441 tatccagaag aaagtgtcat gattggataa tttcatcttc tgacagaaac ctagtgattt
    34501 agtaaaataa gaaaataaaa taatttttct tgcttcttgg atactttaag tgcaaaatgt
    34561 tcgtttattt tttgaggcac tatccgttgt tgaacctcgg gtagtgcctc tttcttattg
    34621 aaaggaatca ataagatttg attcctttat ttatcaatag gttataattc atattttttt
    34681 attttttctt ttacgatact tagtccagaa gctaaaattt caaggttatt agaggatagg
    34741 gaaattcgaa cgaatgcatc tggttgcgca cgtgtagtaa agcgtctgga atgataaagt
    34801 cggataccgt tagcaagaaa gtcattctca attatcttag gatcaatctc aggatcaact
    34861 ggaagaattc gatagtaagg gacacgattc gttggttttt ctactttgaa aagagaatca
    34921 aaaagctgat tggctctttt cgtaagactt aatttagttt ctaagatcct atttgcttca
    34981 tttgaatgga ggatttgagt gatgacttcc acatctaatc cagaagtttt cacatttata
    35041 ttaaaaattg cttgttctat cttgttttgg acatgtactg gaactacaag ataagcaact
    35101 cgcaatccag aacaaataaa ttttgtcatt ccagctaagt agattgtttg gttaggtaat
    35161 aatttttgga aaggaggaat gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata
    35221 tcatcttcaa taacccatag accttctttt tcaatcactt cggctaattg ctctcgtctt
    35281 ttgtgagaga tttgaaaccc tataggatta ttacaagaag gcataagaaa gacaccatga
    35341 atcttctttt ttctacattc ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa
    35401 tcaattggaa cgatctctaa atgatataac tgagccaatt caatgaaatt agcgtatgta
    35461 tatcggtctg ttgctattct atcacctgga gagaaaagtg cagaaagtgt gatagccaat
    35521 ccattttgaa caccggaaac aatagataga gattctaagg acggaatgac tccttgagtt
    35581 tttaaccaat ttgctgcagt agtcaattga aaatttgtgc ctttgggatc ttggtaatta
    35641 agcaaaccaa caagatttgg gttattacta gttgattgaa taaacgggac aacgaattta
    35701 ttacattctt caaaagaact aaccagacca aaatcaatga tatgttcggt tacttcattg
    35761 gtagagatgg tgatggactc aaagacattg ggagaaataa atgtgccact tccaatattc
    35821 gtatacagca aacctttctc aattccatat ttataagcct tgccaacggt agtaaaattt
    35881 aaatctaaat aatcagctag ctctctttgg gagggtaact gagtgttttt ctgtaattta
    35941 ttctttaaaa tatcttgttc taattgttga atcaacgatc gataataagg acgcttcaat
    36001 ttttgtttgt ctggtcgcca agttaacggg taattgtcat aagaattaat aggcattttc
    36061 tacactcctt aaaattgtat accatacaat tttaccattg attgtattga ttttagaagt
    36121 taatcttttg tgaggaggga attgtctatg aaaattatta attttgagga agtagtagct
    36181 tcttttagtt ttgaagaagc gattcaagta atgaaaaaat gtttttttga ttatgaaagt
    36241 ggtaaaatca gccaaaatcc tcgattggtg gaaattttac ctgacggaaa agacaaaaat
    36301 atgtttgcga tgatgcctgc ttatttgggg gagaacagat attttggaac aaaagtcatt
    36361 accgcttttc cggataatca tcagtatcat ttaccttctc atttaggaga gattttcttg
    36421 tttgatgctc aaaacggact acccaaagca ttgataaacg caaatgcagt cacctggatt
    36481 cgaacagctg ctgtatctgc gctggcaaca gattacttag caaatccgca tgcagaaaca
    36541 cttgctttaa ttggagctgg tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt
    36601 cgtcccatca ctacagtgtt tgtttacgat attttagaag aaagacgaaa tacttttatc
    36661 gctaaagcta aaaaacagta tcctaaaata acgtttataa atgcaatatc agtagaagat
    36721 gcagtacgtc cagcggaaat catttgtacg ttgacctcta gtaaagaggc atttcttcaa
    36781 aaagagtggg taagggaaga cgcacatatc aatgctgtgg gcacatttac tcctgataca
    36841 agagagataa gtacatcgtt gatggaaact tctcaaatct atgtagatga ttactcagct
    36901 accttaacag aaagcgggga cctgcttata gccatggcag aaggagattt agaggaatct
    36961 tgtattgttg gtacattagg cgaattagtt tctgagaaaa aaacatttta taaaggagaa
    37021 tcgaggccga cgattttcga cgcagttggt ctagctattg aagatctttg ttgtgcagaa
    37081 tatatttata acaaattaga gaaaggggct ttaaaatgaa cttagattat tacgtagttg
    37141 atgcatttgc agatgaggtg ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat
    37201 ggctgccaga aggtacgatg caaaaaattg ccattgaaaa caatttatct gaaacagcat
    37261 ttacagtgaa gaaaaaccaa gaatttgagc ttagatggtt tacgccagat cgagaaatcg
    37321 atctttgtgg tcacgcgaca ttagcaacag catttgtttt atttaactat tacaaaatac
    37381 ctgatgaaac gattaaattt tctactcaaa gcggtaatct atttgtgaca agaattaaag
    37441 attattatta catggatttt cctagtatta tgccgaaaaa agtgccgata ttgactgaat
    37501 atgaagaagc aattggggct aagattaaag aagcctactt agcacgtgat ttatttttcg
    37561 tattagaaga tgaaaaaacg gtggctaaat tgcagccaga ttttacagct atcaaaaatt
    37621 ttgaattagg aatagagtca tcgtgacagc agaaagtgtg caaaaagact ttgtttctag
    37681 aaccttcttt ccaaaattaa caatcaatga agatccagta tgtggatcag cacattctaa
    37741 tcttattcct tattgggcag aaagaatgaa taaaacaaaa ttgtcagctt atcaaatgtc
    37801 atcaaggggc ggggatctag aatgtgaatt aaaagaagat cgtgtgataa taggaggaaa
    37861 agcagttttg ttttctaaag gtacctcgtt tatttgattt ctttcaaatg aattaaaaat
    37921 aaattggtgg agagtggcag ccttatagct acaatggttg ttaaaaaaag acgcaaactt
    37981 tttcaattta atgaaaagtt tgcgtctttt tttaattatt ggtgtttgtt ttttattcaa
    38041 tcaagagtaa aatgccccat actaactatc ctcggatgac ttcgatttta taaccatcag
    38101 ggtcaatgac aaagtagtaa gaaggcgtag tgccaggtaa tccttttaag tcagtaatct
    38161 caagacctgc agctttatgt tgctcgtgta atttttctaa atcatcagct gcaatagcga
    38221 tatgaccata gccgtctcct agatcatacg caccatgatc atagttgtaa gtcaactcta
    38281 attcatagtc atctccagga agtgtcaaat aaacaagtgt aaatttgttt tctggaaaat
    38341 cacggcggcg gctttcttca aaaccaaatg ccttttgata aaattcgatt gatgcttcaa
    38401 gatctttcac ccgaacacat gtgtgagcca ttttcataaa ttcatctctc cttttccttt
    38461 tactcaatca taacaaaaag aagtaagaaa atagttgatt tgtgcttgtt catgagaaat
    38521 ttctcatgta aaatggatag aaaataatac tgttaaggtg gaaacaattc atggataaac
    38581 caatttttgg tgaaaaaaag caatctgcta attatcaaac ccgttatgca gcatatattg
    38641 tcatagaaag agacaagcgg attgcggtga tcgaggctcc aaatggtgct tttttccttc
    38701 ctggtgggga aatcgaagga gaagagacga aagaagaagc gattgtccgt gagctgatag
    38761 aagaaatggg aattgtggca gaaatttctt cttatctggg agaagctgat gagtattttt
    38821 actcgaatta tcgtgcaact tattattata atccaggata tttctttgtt gctgattctt
    38881 ggaagcgtat aggagaacca actgaaaaga ccaataaaat ttggtgggtg agccctcaag
    38941 aagcgcttgt gaaattgaaa cgtggtagcc accgatgggc agtcgagaga tggatcgaaa
    39001 aaaataaaaa ctgaaagaaa gcgagtagat cagctttctc tcagtttttt tatttattac
    39061 agtacatatt ttttgattgc ttcagctaca ccgtgctcgt cgtttgatga agtgatgaca
    39121 tttgctgctt ctttgacaag agaaacggca tttcccattg ctactcctag accagcatat
    39181 tcaatcatgg ataagtcgtt ctcgttatct ccgatagcca tgatttcatc ttgatcaata
    39241 ccaaggtgct gtgcgagatt cgcaactgcc gcacctttac ttgcttcttt atttagtact
    39301 tcgaagtaat agggcgcact tttgaccgtt gtgtatttat cacggaaagt ttgcggtaaa
    39361 cgcgcaattg ctgcatctaa aatttctggt tcgtcgatca tcatcatttt gacgatgttc
    39421 atgtctggtg tcatttcttc aggtgttcga tattttagcg gcatcttgac taaacttgct
    39481 tcgtggatcg tataagcgct gatatctcga ttaggcgtgt agatcgcttt gtcatcgatc
    39541 gaatggagat gactgccgac acggcgtgcc atcacttcga tctctaggaa atcttcatga
    39601 gtcagtccgt gacgggcgat gattttacct gatttcgttt gttgtacgag tgccccgtta
    39661 taagtaataa cgtaatcatt atcttgaaaa aggttcaact cgtttaactg ttcctggaca
    39721 cctggtaagg gacggccagt gcataaaacg atcgaaacac cttttgcaac agcagcggca
    39781 atcgcttctt ttacttcttt tgttacttct cttttctcat ttaataaagt accatcgatg
    39841 tcgatagcga ctagtttgat agacataatt tcctccaata tattttaatt tatttcaatc
    39901 aatgcgccat tacggatatg gctggcaaat tcttgataag tttcgtcaaa taaatcataa
    39961 tgatcacgca aggagggatc aaccatttct tttggaaaga aaaatcgctc atctccttgt
    40021 tcttgtcccg ctaatgcagc aacaagctga ctaacctgtg ataattctat caaagttccg
    40081 tcatttcgca aaatctcgat ttgtgtgcga tgtctgcctt gttctgggcg ataaaaatca
    40141 taaggcagat cgtagctaga attcacagca gtgtaataaa cagggttata gccaaccttt
    40201 tctactaaca aagtgagttc ttggactaat tttgaatccc ggttgccaga aaaagtagca
    40261 gatttcagtg gtttacgatt caaaaaacgt ttagctaaat catttaaaat aggatcagca
    40321 gattcagacc attgagtaaa gtaagtgccc aagaccccat catcaagttt gaggtattct
    40381 tctaaagtaa agcttccgtc aagaaatggc agaagtaatt gagaatggag ttcaaaactg
    40441 ctggcatctt cttgatacaa ctcgtgtgcg cgatgaagca gatgctccaa aatcacttcc
    40501 ataccacggg aaacaggatg gaagtacact tgcacataca tttgatatcg gctaacgata
    40561 taatcttcca ctgcatgcat gccactcatg gaaaaagcga tcccgccttc ataaggacga
    40621 atcacacgta atattcgggt aagatcaaat gttccatatt ctgttccagt gaaataagca
    40681 tcccgaagca aatagtccat tcgatctgca tcgatctgac tagaaatcat ttggacaact
    40741 tgtgggttgg ggtaagtttt ttggatcaca ctagctacct tttcaggaaa accttcttct
    40801 actcggttaa gaatctgata cacttctgtt tctggagaag tgatgatctc tacagtgatg
    40861 gcttcatgat tcgtatggaa gatatgttca aatgtatgag agtaaggacc atgaccgaca
    40921 tcatgcaaca gtgccgcaca taaagtcacc agccgttcct gatcatccca gcctccgttg
    40981 cctatttttt cttttgaaaa atttcgagaa aagatatcgc agattcgtct tgaaatctca
    41041 taaacgccta atgaatgggt aaaacggcta tgttctgctc cgtgaaaagt gaaagaagaa
    41101 gtgcctaatt gtttaatgcg tcgtaaacgt tggacttctt tcgaattgat caaatctaag
    41161 attactttat gctggacatg gatatagtta tggactggat cacgaaaaac tttttccata
    41221 ggcagcattt gatatttata aggggttgtc ataatgtagc tcctttatgt tattacgttg
    41281 ttccattctc tggatttggt tgttccattg ctccagctgg atttcttttt ttagccagtc
    41341 ggtagaatcc tgtaaatcac ttgcagaata cagcttttca aaagcttttg caaatgcttc
    41401 tttgacttct tttacaaaaa ggggttggtc aagcagttct tcaagtgttg ccattgtagc
    41461 aggatccact gcaggatagc catttgtacc aaagtcttct tttaaagcag attgataaaa
    41521 tcgcttcatg acttgcccgc gtttttcctg attaccgttg acacttaagt acatcataac
    41581 agaaatccca tttttgatcc gacgctgagc gataccggca aattttttgc catggatgct
    41641 taaatcataa gttccaggac aataagaatg ggtgatttcg aatgcttgta tcggtgcctt
    41701 tggaaaagcg tatttagtca ataggagcat ttgctcgtaa gcgtcatcta tggttatttt
    41761 ttttgtgtca ctttgaggga aaatcaaaga aatatttaaa attccttcat ctgatacgat
    41821 ccctaatcca cctgaatttc tgatcactgg agcgtaacct gcatctttca gtgtttggat
    41881 cccgtcactt agaaaaggag ttcgaacatc cttcatacct aagatgactg tgcagtcata
    41941 ttgccagaaa tgtaaaaaga tttgctggtg ttctttagaa tattccgtta atatatctgt
    42001 taatgcaaaa ggggaaaagt cgtttttttg ataaatgtgc tgatctagtc gaatcgcagg
    42061 gttcattcaa ccggctcctt tgtataaatt catattttct acaatattat actctaaaat
    42121 caaagagttg ttttggaaaa aaggttttct gtaaaaatgg acaagttggt tgacaaaaca
    42181 aaagaaacta ggaacaatgt agctatgaga agtaaaggag aatgaataat ggaattatca
    42241 caaggaacac cgatttcaat caaattgcgt acaaaagtaa aacagcaagg ggaagtgcaa
    42301 gatttttatt ttgatttgaa agggcaaatg gtgacaattg gcgatacgct gtacatacgt
    42361 tataaagaga ttcaggagga gacgggcgat gagattcctg taacgatcaa attagttcca
    42421 gatggtcatg tacagctgat ccgtgcagga gaaatgcgga tgcgtttgaa atttggctat
    42481 aaagaacggt tagaaacatc ctatcgaaca ccatacggta tgatccagat tgctactttt
    42541 acgaaagaac tgcatgttag tttgaaagac cgtcccacag cagggaaggt tcgaatcgat
    42601 tatgatctgt tcatgggacc ggaaaaaatc ggcgaatatt atttgacctt ggattttaca
    42661 gcgtaaatat aaaagaaaag tttgattttt tgtgtctgat tttgtatgat aaggagtaga
    42721 ctgtgaaagg acgtgtcctg attggaaatt agtgtatttg aaggtgcaaa caaaagcgaa
    42781 ttatcaatga tcgaagtagc acacgcgatc ttagaacaac gtggcgatgt catggatttt
    42841 tctgatttag ctaaccagat ccaaaactat cttgagaaat cagacagtga aattcgtgat
    42901 tcactcgcac aattttatac tgacttgaat attgacggca gctttatctc attaggggat
    42961 aatcgttggg gcttgcgttc atggtatgct atcgattcga tcgatgaaga agtaaaccat
    43021 ggaatggacg aagaggatga agatactcca cgtcgccgta aacgcaagaa agtcaacgcc
    43081 tttatcaatg atgatgaaga cgcgatcgat tacaatgacg atgacccaga agatacagat
    43141 ctgacagaag aagatgacga tgatctgttt gatgacgatg atgatgaaga tgaagaaatc
    43201 gctgcttata attctgactt gcaagaaatc ggagcagatg acccgacaga tgatgaagaa
    43261 gacttgccag gaaatatcga agaagattta agtatcatag acgaagacga tgatgatgaa
    43321 gattatgaag aggaagaatt ttctgactct gaaaaagaat aaacattatt ttcttacgag
    43381 aggttgtgac agagacgatc agcctatcag aataaggagg tgtgacacga tttttgtcac
    43441 accttctttt ttgtatataa aaatcccaag taaatacata aaaaataaaa aaattgaaac
    43501 ttttagcgca ccttgtacgt catattaagt gaggcagaaa ggagtggaga atgattttta
    43561 ctggatataa gaaaaaacgg cagatcgaaa aataccgtca cctgctgaat ggactcatcg
    43621 aaactgatta tcaaaaaatg ttccgtatca cgatgaatta cgtacatgat aaagaagagg
    43681 ccttagatgt gatgcaagat agttttcata aagccttgaa ctattttgaa aaagaaaaag
    43741 agatcgagca ttttagtgca tggttttatc ggatattgat ccgcacggca ttagatagtt
    43801 ggcgcagaca aaaaaggacg cctgtggact tatttgaaat tcaagatatc ccccaacagt
    43861 tacctttaga aacttccgtt gcagagctcc atatgatttt agataaaata gacagtccgg
    43921 aaaaagagat tctgatcctc tacttttttg aaggatttcg tttgaaagaa attgcaacta
    43981 tcttggatat gaatgaaaac acagttaaaa cgaaaatgta ccgctcattg aatgctttac
    44041 gtcaaatcct agaatgaagg agagaagagt gccatgtatt cccaaaaaga aaatttaaaa
    44101 aaactaaaga aaacttataa agaacaagca attcccaaag ggcttaaaga ggagatccgc
    44161 cagcgtttta ttatcgaaga gcaatcattt ataaaagcaa ggcggagaaa gagacgtctc
    44221 caagggttcg gagtatcttt ggctatcgta atgattacaa caagtagcct tctttttaat
    44281 agtcaagttc gaagctttgc agaagattta cccatcttag gatcagtcat tgaattgatc
    44341 ttaggcgaac gctttactga tcgatctgaa aaaatcgata ttcaggtacc gaagatcaat
    44401 acacaaaatg agaaagaaaa taaaacaatt catggattaa atcaaaaata ctttcgcgaa
    44461 ggacaagcag attttgaaaa agctgaaaaa gaatatggaa attttgaaac ggatcattac
    44521 caagttttgg gagattatca gaaaatcgta gacgataacc ggtttctcgt gattgaaagg
    44581 cagatcaccc aaacagcagc agatagccat gttgaaaaaa gatacgacac aatcgacaag
    44641 aaaaacagtg tccagctctc acttccactt ttattcaaag atgacactta tctatctgtt
    44701 ttgacaaaag aagtcaaacg gcagatggcc gaacaagtca aggaagatcc atctaagtac
    44761 tattggactg agcaagatat tcaagaaggg acgatagaaa aaccaacact tgttacgcct
    44821 acacgaagtt tttatttgaa caaagagcat caattagttt tgactttttc tcaatacgaa
    44881 attgctcctg gttatatggg tacaccagaa tttgtcattc ctaagtccgt gacaaaaacg
    44941 attttggcat cggaagatta tttggatcat tagacgagaa gtagttgcga ctagcagttt
    45001 caattttcaa gtaagtacaa gtaaataata agaagagaaa aagagaaaga agagatatag
    45061 tgaaaagaaa ttatttagtc atcagtatta ttgctttatc cgtgcttttg tttttcgaaa
    45121 ttacttcggt ggcctccttg atcaccgaag taaaaggaca aacacactct tcggagcatt
    45181 cgtcacaatc tttcaaacaa tcagaacaat caaccattaa aagccaatca actgaaacag
    45241 ctaacgtcaa agaagccatt caagaatcag cggaaaaggt acaaagtcaa tcatcacaat
    45301 caacacaaga cagtacagaa gcaatccaag acccgactgt caaaagcgtc gcaatcagct
    45361 ttgatgatgg accaggagcg accacaacgc cgcagcttct gcgaatctta aaagaaaaaa
    45421 acgtgcatgt cacttttttc gtcttaggag aaaatacggc gcagcatcct gaaatcgtga
    45481 agcagacagc tgaagcaggg cacgagattg gcaatcacac atacgatcat cagaatctgg
    45541 ccactctttc tgcccagtcg atgacggagg aagtgacgaa agcagacact gagatcaaaa
    45601 aaagtcactg gcaaaacccc cgcttttgtt cgtccgccct atggttctgt cacgaatgta
    45661 ggagcaacga tcatccagcg accgatcatc gaatggtctg ttgattcaga agattggaag
    45721 acaaggaatc ctgatttgat tttgcagaag atccaagcaa cagtatacga tgggtcaatt
    45781 attttattcc atgatatcta tcctgaaacg attcgtgccg ttcctcaagt gatcgattat
    45841 ctgaaagagc aaggctatcg catcacgaca gttagcgatt tattagggca tccaacggct
    45901 gtcgagaact attatggcag aaacgatcat cggccagttc agtaagtgag acaagtatct
    45961 ttaataaata acacatttta ataagtagtt gaacacataa ctaccttact tgcaaaggct
    46021 taacaata
//
LOCUS       DAZUMM010000012        45449 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000012 DAZUMM010000000
VERSION     DAZUMM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.012"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(175..1485)
                     /locus_tag="ACG8E6_000623"
     CDS             complement(175..1485)
                     /locus_tag="ACG8E6_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFS5061068.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1497..2915)
                     /locus_tag="ACG8E6_000624"
     CDS             complement(1497..2915)
                     /locus_tag="ACG8E6_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5061069.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3155..4723)
                     /locus_tag="ACG8E6_000625"
     CDS             complement(3155..4723)
                     /locus_tag="ACG8E6_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFS5061070.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4727..5137)
                     /locus_tag="ACG8E6_000626"
     CDS             complement(4727..5137)
                     /locus_tag="ACG8E6_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS5061071.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5134..6057)
                     /locus_tag="ACG8E6_000627"
     CDS             complement(5134..6057)
                     /locus_tag="ACG8E6_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFS5061072.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6054..6932)
                     /locus_tag="ACG8E6_000628"
     CDS             complement(6054..6932)
                     /locus_tag="ACG8E6_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFS5061073.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6919..8256)
                     /locus_tag="ACG8E6_000629"
     CDS             complement(6919..8256)
                     /locus_tag="ACG8E6_000629"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFS5061074.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8259..9362)
                     /locus_tag="ACG8E6_000630"
     CDS             complement(8259..9362)
                     /locus_tag="ACG8E6_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5061075.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9436..10944)
                     /locus_tag="ACG8E6_000631"
     CDS             complement(9436..10944)
                     /locus_tag="ACG8E6_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5061076.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDTEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10910..11602)
                     /locus_tag="ACG8E6_000632"
     CDS             complement(10910..11602)
                     /locus_tag="ACG8E6_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS5061077.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11624..12598)
                     /locus_tag="ACG8E6_000633"
     CDS             complement(11624..12598)
                     /locus_tag="ACG8E6_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5061078.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12898..13770
                     /locus_tag="ACG8E6_000634"
     CDS             12898..13770
                     /locus_tag="ACG8E6_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5061079.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14228..16426)
                     /locus_tag="ACG8E6_000635"
     CDS             complement(14228..16426)
                     /locus_tag="ACG8E6_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5061080.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16658..17650
                     /locus_tag="ACG8E6_000636"
     CDS             16658..17650
                     /locus_tag="ACG8E6_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5061081.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17710..19131
                     /locus_tag="ACG8E6_000637"
     CDS             17710..19131
                     /locus_tag="ACG8E6_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFS5061082.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19124..20623
                     /locus_tag="ACG8E6_000638"
     CDS             19124..20623
                     /locus_tag="ACG8E6_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFS5061083.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20662..22056
                     /gene="uxaC"
                     /locus_tag="ACG8E6_000639"
     CDS             20662..22056
                     /gene="uxaC"
                     /locus_tag="ACG8E6_000639"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS5061084.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22215..22898
                     /locus_tag="ACG8E6_000640"
     CDS             22215..22898
                     /locus_tag="ACG8E6_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFS5061085.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22900..23736
                     /locus_tag="ACG8E6_000641"
     CDS             22900..23736
                     /locus_tag="ACG8E6_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS5061086.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23747..25501
                     /locus_tag="ACG8E6_000642"
     CDS             23747..25501
                     /locus_tag="ACG8E6_000642"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5061087.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25777..26118)
                     /locus_tag="ACG8E6_000643"
     CDS             complement(25777..26118)
                     /locus_tag="ACG8E6_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS5061088.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26186..28357)
                     /locus_tag="ACG8E6_000644"
     CDS             complement(26186..28357)
                     /locus_tag="ACG8E6_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS5061089.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28568..29425
                     /locus_tag="ACG8E6_000645"
     CDS             28568..29425
                     /locus_tag="ACG8E6_000645"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS5061090.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29496..30353)
                     /locus_tag="ACG8E6_000646"
     CDS             complement(29496..30353)
                     /locus_tag="ACG8E6_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5061091.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30338..33040)
                     /locus_tag="ACG8E6_000647"
     CDS             complement(30338..33040)
                     /locus_tag="ACG8E6_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS5061092.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33053..34342)
                     /locus_tag="ACG8E6_000648"
     CDS             complement(33053..34342)
                     /locus_tag="ACG8E6_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFS5061093.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34492..35538
                     /locus_tag="ACG8E6_000649"
     CDS             34492..35538
                     /locus_tag="ACG8E6_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5061094.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35540..37693
                     /locus_tag="ACG8E6_000650"
     CDS             35540..37693
                     /locus_tag="ACG8E6_000650"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFS5061095.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38166..39617)
                     /locus_tag="ACG8E6_000651"
     CDS             complement(38166..39617)
                     /locus_tag="ACG8E6_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5061096.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39614..41338)
                     /locus_tag="ACG8E6_000652"
     CDS             complement(39614..41338)
                     /locus_tag="ACG8E6_000652"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5061097.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41331..41945)
                     /locus_tag="ACG8E6_000653"
     CDS             complement(41331..41945)
                     /locus_tag="ACG8E6_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5061098.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42290..43747)
                     /locus_tag="ACG8E6_000654"
     CDS             complement(42290..43747)
                     /locus_tag="ACG8E6_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5061099.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(43788..44708)
                     /locus_tag="ACG8E6_000655"
     CDS             complement(43788..44708)
                     /locus_tag="ACG8E6_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5061100.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(44720..>45449)
                     /locus_tag="ACG8E6_000656"
     CDS             complement(44720..>45449)
                     /locus_tag="ACG8E6_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5061101.1"
                     /translation="LFSSDQAFLITKNTILYNVAFILLNLLISVVFAIIMSELRNKRL
                     VKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFI
                     LVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTIL
                     TILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQS
                     VVGFVLLMITNTIARRFDSDSALF"
BASE COUNT        13413 a         9295 c         8006 g        14735 t
ORIGIN      
        1 caagtggttc tttttaacga cttagttatc ttatcacgtc taaaaacatg tgtcaactct
       61 tttttggaat tttcataaaa tgtttcaatg aagcgcgaca acgttgttta atatatcatt
      121 aaaaaaaata aaggtcaagc taaaatatta tttaagcttg accttattat aatttcaacg
      181 attgtcaaaa gtattgtttg cagctgtgtt gccagtttct atagataaat cacagctttt
      241 gaaacataag ttttgtactg cttcgattcg cccgaattct tttgcagtta tttggcaatg
      301 agaaaagcag acatcttcta taggcttttc aggaaatgct actaaatcaa aagctcggga
      361 aggaagtgag taacctggag aaaggcatgc ctgtacgttt ttgatgtgta tgtctttgac
      421 tttcgtcatt tgctttgatt caggtatttc ttcagccact gcttgccaac tttctggcat
      481 ttgagcaagg tcattgctat tttttctatt gtattcatta tgccagtcca ttatccaaga
      541 aaaaggaaat tgtacatcaa tcatattcag attttctacc ctgatatttt cgatcactcc
      601 ccctctttct tttgaagatt tcattcgaat accacagtct gactgaaaaa aatcgatatc
      661 gtgaatatat acgtcactaa ctccagcact aacttcgcta ccaatagtta caccgtatcc
      721 tgaacggatt ttacatctag caacctcgat tcttgatgat tttcgattga ctcttgcacc
      781 attgccatct ctgccagact taatagcaat acaatcatca ccacaggcca aatcacattc
      841 atagacacgc acatttgttg aagaatcaat atcaatccca tctgtacttg gtccatcatt
      901 gttgcggatg attacttttt ctactaatac atcgttagaa taagtgattt gcaagttcca
      961 aaaacctgat ttctgaaaaa tcaaatcacg aacaacaaca tgttctgcat gatacagtaa
     1021 acaagcgcgt ggacgtttaa tagcataatc cgcaatccaa cgaagtccct tttgatcgta
     1081 ttcagctctt gtcccgcttt tttgatcttt tccccaatac agctcccacc aatggtctcc
     1141 ttgaccgtcg attattcctt ttccttctat aagaatatct ttcgccgaga gaacattcaa
     1201 aatcgctgct ggccatttca tttctacgcc ggctactcgt gtatcgattt ctggaaatgc
     1261 ttcgatatct gttctcccct tgaggactgc accttcttca aagataagat gactcttatc
     1321 ttttaaaaaa agggctccga ctaaataaat gccttttgga ataaatacag gtaagtgttc
     1381 tttcgcacct tgatcgattg ctttttgcag tattattgta tcatcttgtt gagtgtcgtt
     1441 tgttttaaag ttcaaagcat tgataatccc tttttttgtc cgcaatacgt ttctccctat
     1501 ctttcattga tttttttcaa ttcttcacca ataaatacag tttttgaacg attcagtggg
     1561 taacctacta gcaaaatgat tgcggataca aataagcaga atgctggtaa caagtttgcc
     1621 atcgtataaa tcctttcttg tactaaaact gattggatgg ctccacctgt tgtagacgat
     1681 tggtaaccaa tgagtgccag cataaatccg cctaccgctc ctgcacatgc ttgtcctact
     1741 tttctcgcaa aagaatttac tccatatact gttccatctt ctcttaagcc tgtcttatat
     1801 tgatgataat caatcacgtc agtaatgaat gcccagacca tcaaattgaa taaaccagat
     1861 cctaaagtag cgataaataa aagaatcaga tatacccatg catttgttat atgaaggaaa
     1921 tacatcagca agtacataca tgaagaagca agtaacgcaa atacgcttgc ttccttttta
     1981 ccaaataatt ttgtcagctt tgtagcaaat ggtgctaaaa ctactactgt tccgtaagta
     2041 aataaaagag caacagacat tgcttgttta ttatggaagt aatcattgaa taaataagtc
     2101 atattcgtcc cagctaaaat ctgattgatc acaatgaaaa tgtcaacaat gactaaaata
     2161 atcagtgctc gattagctaa caaacctttt accaactgag aagcagatac tttttcgttt
     2221 ttgacaactc ttactctttc agtagtcaat ttacaagtta acatatatgc gacaaatgct
     2281 attgcagcac atccgattga aatccaaaag aaatgattcc ctgaaacgac ttgttgtcca
     2341 ctcttgttat gggtatacat taaaatcgga atgaagaatc ctgtagttga tccgccaatc
     2401 gcagaaccaa tgcttcggaa cgtggacaac gaggtgcggt cattaggatt tgagctaatt
     2461 gctgctgcca ttgctccata cgggatattg attgttgaga gaaaaatccc ataaacaatg
     2521 tatgaaacaa aaatataagc aatcttcacc gtcattggaa gatgttggac gaatggcaaa
     2581 aacaaaatga cagtcactaa gcaaaatgga tacttcattt ttctgatcca aggaataaat
     2641 cggccagttt ctgttaactc tgagacatca cataatcttc caaccatcac atcagcaaac
     2701 gcatccaaaa ttctcgaaat caagaataac gtacctacga ttgcccctga aacgccaaga
     2761 acattcgtat aataaatcat caagaaacta ttgactaaac ctaaaataaa acagttacct
     2821 agatcaccga acatgtagcc tatcttatcg gaaatcccaa attccttttt ttcttttttt
     2881 attgtatttg ctgtaacagc attttgtgaa agcatttgaa ttcctcctac ctcttctagt
     2941 aaacgttttc aaaattataa ctcttttttt cttgttttta tattcacaat gttggctttt
     3001 tattgcatta tcttgacaga taaaaaacaa aaaataatct aaaaacattg atgcataaga
     3061 ttgataactt tttttacata acaatatata caaacaaaaa aattcactaa ctagtgaaaa
     3121 taagttagtg aacctcattt attaaagaat taaattacag atgatctttt ccatcatact
     3181 tgaaaaactc aaaatcagca taacttcctc tttttttatt tagatcatgc actgctatcc
     3241 cgataaaatt tccagtaaac cctcctgaga gtatacacat gtttttttgc ggacctactt
     3301 tcttccattc ttcagttgat acgtctttca aatgaaactg tgctgtctct ttctttacag
     3361 tgactttcaa aaaagtcttc ttttctgtga gtggaatcgt ccaatcttct atttgtgcta
     3421 ctccgttttc tgattctatc atacgtaggc attttccctt gtcctcattc caagtaacat
     3481 aacaataaat ataattggat tcatttaagt acagtaccag tccagccatc tgattaaaat
     3541 tattaggcat gtattcaaat gctgtctccg catcaaaagt aaaatccgtt tgccgaatag
     3601 ctaacaaatg atgttcgaag gtagattgaa gagattcacc ggaaatcaga cgtaaaaaac
     3661 ctggtctaca ttttgtatca caccaatcag catttggcaa aatgcgtctg gcattccagt
     3721 tcttctttag ctctccttca aatgtatcca taaactgatg atctttttct tgttgtacct
     3781 cttcatttgt ctcaatcatt atttcttcca tcggagttgt ctggctatct ttaagtctta
     3841 accaatcgtc ggacgtccaa tacacttctt gaatagcggt ttctcttccc aaaatagccg
     3901 caccgtttaa tggtctggta caaagatatg tcatgtacca tcttcctgac ggggtttgta
     3961 ctaagctccc atgtcctgta cattgcaatg tagaagcagg tttgtcacta gctgtcagca
     4021 taggaaaacc aggatcaagc tcgtaaggac cagtaatatt ctttgctcgg caaactgtaa
     4081 ctgaatgtcc tcttcctgta ccgccttctg ccgtcatcaa ataataatac ccgttacgaa
     4141 aatacaaatg tggtgcctcc gttttcgcta attctgtacc tgcaaatatt ttatagatgg
     4201 gaccatcaag cttcttcttc tctgcatcat attcttgtaa tacgataccg ctggatttat
     4261 tcggtgtgtc cagacgataa tcccatagtt cattgacaag ccattttttt cccgtttcaa
     4321 gatcatggaa caaagaagga tcaaagccgt tactatttaa ataaattggt tcggaccaag
     4381 gacctgtgat ctcagatgca gtgatcaaat aattgtggac atctttaaac ggacggcctg
     4441 cacttttgac atctgtgtaa acaagataaa acaatccatc agcataactg atctgtggcg
     4501 cccatatact tccatttgta ggatttcctt gcaaagatac ttgagtggtc aaaatatccg
     4561 ttgtatgtga ccagttgact aaatttttcg actcatatac tcggattcct ggaagccatt
     4621 caaaagagga gacaataatg taataccgct ctttcactct taatatatta ggatctggat
     4681 tgaatcctct taaaatagga tttttcactt tgatagtctg catgattcag gctccttttc
     4741 tcattcttac tcgatataaa ctgaatgccc cagctgcgaa ataaaaaaat gcaaaaggaa
     4801 acgcaataaa ctgagtgcca atcaacaata tgaatcctgt tataaaatac aaaccagtgg
     4861 cagttgttaa gtattttcct ttagcagtca aaaacaatgc tactgctaaa aaagcaagtg
     4921 aaataaacaa cacaacggca aacagaccag tcatgtcctg taataatcca aatgtatttt
     4981 ctaaagacaa agttgaatga tctacagcaa gcgactgata acttttttta aactctgacg
     5041 cagacatatt acttaaagta actgctaacc cgccaaagaa aatcaaagat agactgccac
     5101 cgaccattcc tagcaatagt tcccattttc ttttcatttc tgaaccccct gcttttctaa
     5161 atgtatccat tcaggtcttt tgatcgatgt ttcataattg ttattacgtt cagagaattt
     5221 gacagtctgt tcatttttct tgtcatccca attatcccaa ccagttgggt gaacatgccc
     5281 acctatctta cacgcttcaa ataagacatt tgcatacgga cgccacggtc ttcctaaata
     5341 gacgtttcta gtatcaggtt ctgcagtcaa ggtacagctt tcaaaacgat accctgtttt
     5401 ttgatttttc ggcgtagatg ctgctgtgat gtatccagga tttttactgg ctaagctatg
     5461 gatatgacac tgatgaaaaa cggcatctgc cccaccaaat atgaaatcca ccgttccttc
     5521 aatccagcaa tattcatagg tctgacgaca atactcagga ttggcagaag ccggtgtaca
     5581 aaaaggagta ccatcttttt gcaaatcagg caacggaccc gtgcataagg tatcctgata
     5641 accagatagt cgacagtttt ttaaagatac gcgatctcct gaattgaata aagcaacagc
     5701 ctgtcctacc ttttctcctg gacctgctgt attttcgatg tgaagatttt ctaatacgac
     5761 attcgatcct tctaagaaaa aagtagcact tcggaaagtc cctaactggc taccgtcaag
     5821 atactcctgt tttgcaaaac gattaccgga gataactact gttcctgctc ccacaagttt
     5881 aaaattggat aatctgcttt ctatatattc atcatatctt ccttctttta ctatcaagac
     5941 ctttggatgg ctatccggca ggcctgccaa ataatcgatt ccttcttgga tagtttcaaa
     6001 atccgtcggg gtatctatag cattctttcc aattatcagt ttgtctttca tcctcataaa
     6061 tgctccctta tgaattgttt ccaaatattt ctcatttctt gtgtttccat atgaccgcct
     6121 gttaaagaaa aagaagaaaa gttttctgga ctacccattg attgatactt tttgtccagc
     6181 ccttttctca gcttcagtac gccttcgtcc aagcacatcg tatcgttttt tccaactaag
     6241 ctaagacgag ggcgcggagc gattttttct tggattgcta acgttgtata agctgtcaat
     6301 agatttggca catagtaata aaacccatga tgatctaggc ggcgatgtag caaaagtatt
     6361 tcgatgtcca cctgtgccgc tagatcaata caaactttga cacgagaatc caatgctgtc
     6421 acccaccagc ttaataagcc tcccatagaa agaccaatcg ttgcgagccg ttgagtatct
     6481 gtgtcttccc ttgtctccaa ataatcgatc aagctgatca catcataaat acgcattccc
     6541 cataacgttt ttcctgttaa taaaaattgt ttgaacaact cgctttcaga aatacctccg
     6601 cgttcttcaa atccccaagc atcaatcgac caaacagcat atcccattcc tgtcagtact
     6661 tctgcaaaag atggagaagc taagtaagga gcaccatgga tcaattcact tttcccgact
     6721 gaaaaatctc cgccgtgaga atgcaggtaa aggaccggcg gaaacggtcc ttctttttct
     6781 ggataagcat agagggctgg tactgtttcc aattcattta agtacaattg atagtattca
     6841 agcataaaac cgttttgttt ttctcggtat aatcgttttc cactaggggt atgtctgcta
     6901 gatggagcac ctaaaaaatc attcaactgc ataagcaaag tttttacgaa caaccagttc
     6961 atccatattt tctgattgat ttgattggaa tttctcaata ctgatttctt tagtattttc
     7021 taatagaaat gctggtccct cgtgattttc aatcgacatc tggttaaagg aaaccttacc
     7081 cgcaaagcca atataaaatc cttgtctgct catttcctct attcctgtca tcatagcagg
     7141 aacaccggga actgcatctt gggccatcga gacatcgata ttgtcaaatg tgatttcttg
     7201 tatgaattgt tccgctaatc cataaataaa acctgcagaa gcatggacat tccttgccgt
     7261 gatattcgaa aaatggatcc ttctgaatgc tggtgtccgt tcatctatcg gataagcctt
     7321 tttttcccat acatacggtt ctttccctcg cggaccacag aaataataaa gattcagtat
     7381 aaatggacac atcacattat ccatgactat attggatact cgaatatctt caacgatccc
     7441 gcctcgacct cgtctagatt tcaatcgtat tcctcgatca gtttcttgga agatacaatt
     7501 tgaaatggta atattacgaa tactaccact catttcactt cctaatacga caccgccatg
     7561 tccatggacc atcgtgcaat ttgtgatcgt gatattttcg caagcgatcc tttcgtacgt
     7621 atcttcagtc cctgccttga ttgcaataca gtcatctccc acgtcaatat ggcaattact
     7681 aatacgcaca tttttgcatg attctggatc aattccgtct gtatttggtg aatccgctgg
     7741 gttcaagatc gtcaagttat caaacgttgc attactgcaa agaatcgggt tgatcgtcca
     7801 acttggtgac tggatcaatt taatatcttt cacagtaatt cgatgacagt tatggaaact
     7861 catcaattta ggacgaggat aagcgagatt atctggttca tttctaaaag tatgccacca
     7921 tttttttcca tttccatcta atgtaccgaa accagtgaca gagatgttct ctacattttg
     7981 agcatagata caggaagcat aaacttttcg atggactcct tcccaacgtg aatgaacaac
     8041 aggataatct tttggatcgt cagagaattt cagaaccgct cctgcgctta aatgaagttc
     8101 gacattgctt tttaaaaata aagctcctgt tagaaattca cctgctggaa caactacggt
     8161 cccgcctccg tcacttgctg cagcatcaat ggcttgttgt attgcttcag tattcaattc
     8221 atcaattgat gcgccaaatg tcaaaatatc atacatattt ataatgcctc cacttcaatc
     8281 aatgcttgca agaatgcacc aacgcctttt tgatcattac aaataattgg ttcactgatg
     8341 tagtaagcat atgaaccatc tcgctgatct gctccgccta accctgctac ttgacagttt
     8401 ttattcaagt tcagccagcc ttcttttgtt tccagaacaa attcatcaag taatccttga
     8461 tgtgatttct tcacttgtgg aagccattct tctttgttca gcagtcccaa acgtattcct
     8521 ttagccattg cgtaaacaaa catactactg ccggaagctt ccagataatt tcctttccgt
     8581 tttgtttctg ttgttacttg ataccatgtc ccgctgtttt cttcttgata tggaagtaat
     8641 gcagccagtg tcttttctag aatttctttc agcaaagaac aatctgtttc ttcatgaagt
     8701 aattcatagg tatctgctgc agccatcaaa taccagccaa tcgatcggct ccaaaaatga
     8761 tgggatagac ctgtttttgg atcagcccat ggttggacct tcttttcatc ccatgcatga
     8821 atcagcaaat ttgtttttgg atcgaataga tgtttataac tgatttcaaa ctgtctcaaa
     8881 acatcttggt aatctttttc ttcactaaaa ttctggataa attcagcata aaaaggagaa
     8941 cccatatata agccatccaa ccaaatttga taaggataaa tttctttatg ccaaaaagct
     9001 ccttcggatg tgcgtggatg gtttgccaac tgctgggaaa ggagttctgc tgctttttta
     9061 tatttttctt cgcctgtttc tttgtacaat aaaaagaaaa gctttcctgt atttacatgg
     9121 tcgatattgt attcttcaac gctgtagcca cggatcgttc catcttcttg cacgaaatag
     9181 tcgatattgt cttttatgaa atcaaaataa attttttctt gggttcgttc ccaaagtaat
     9241 tggaaccctt taagtacgac gccatagtca taagaccatt ttcctttata ccacttttct
     9301 tcatataagc gaggtgttct ttccatgata ctttttgcta atttctttga ccagttttcc
     9361 attatttgct ctcctttaaa aattaaaata ggatttggga caaataccaa gaagtatttg
     9421 tcccaatcgt ctatattact gtgccgctgc tttgtatgct tcctcgtact cttgtgtgat
     9481 tcgatcgcct ccttgtgctt tccagtcttc tacagctttt ttgaatccat cttcgtcaag
     9541 ctgtcccatg atgtattgca ccgtagcatc aatgacgatt ttttcaattt ctgatcctac
     9601 ttcattgttc gtcggtgaat ccaatggcac agaaggatcc attacagcaa attcttcatt
     9661 ttctttgatc aattgattag agaattcttt ttcagtgtct gtatctttga aagagaacgt
     9721 cacttcacta ggacgagagc ttgataacgg ttgcacctct tgttgccaaa gatccatatc
     9781 gataaattcg atagaaccat catcattcaa ttcataatgc gttccttcga taccgccagt
     9841 catcaattta tacacatctt cttctaaaag atcatcaaca aattgaagta atttcttcaa
     9901 ttgtgcttcg tcttttactt gagatttggg aaatgctagc aagccgccaa taccatttcc
     9961 ttctgaccaa acatgataac cgccgccttc accatctgag atcttgttta caggcactaa
    10021 ttccatatca tcttgaatac cttgacccat cgtcttcaga ttcttgatat ccaccattcc
    10081 agtgtagatt ccagttcttc cttgtgcaaa ttgttcttgc tgatcagtct tttgggtaac
    10141 tgcaaagtct tgtgccaaat agccattctc aaatagatct cttgaaaaat tcaaagcttt
    10201 catatactct tctgtatcaa attctggagt gaatttgcca ttatcgtcta cagcccagcc
    10261 atttggtgct ccaaaataag aagtgaaaag tttaaagctg gtataacgaa tatcactagg
    10321 cgcacgatcg ccaaaaccaa ctgtgtcgtc cacaccattt ccatcaggat cgttttctgt
    10381 aaattgttta gctacttcat aaagctcatc catggtagtt ggaacatcta gtcctagatt
    10441 atccaaccag tcttttcgaa tgactaagcc agcacgagcg tagtcttttt gtatcggtac
    10501 gccgtacaag gcaccgtcga tactcgcagc ttcgattcta tcttcagaaa tttgacttaa
    10561 atttttatag tcttttaaat attctgatac atcccagaat acgcctgatt ttaacgcatt
    10621 gcggacagaa gagtttttca acatcgcaat ttgaagtgta acgatatctc ctagttcatc
    10681 agaagccaat gcagttgtta atctttcttc ttttgaagca tcaggaatcc agttgaatgt
    10741 cagttccgta tcggtttttt cttcgatcaa gttcacaatc gtgtctgtag gcggtgaagc
    10801 atgatgcaaa atatttaacc aagtgatttt agcttttcca tcttctgtct ctgaagaact
    10861 gttacttgaa ctgctgccgc atgcggcaag tgctagaatt cctgcaaaac taagacctaa
    10921 ttttacgagc gttgcttttt tcataaattt tctccttcac tgttttaata atatttttgt
    10981 tttcttctgt tttttctgta tatgagttct tccattccaa atggcaaaat atagaatttg
    11041 ctgtccacgt gactgcggaa aagaagagac tgccaccgat aaaggttaaa acacctggca
    11101 ttgtttggat gatcatcaga taaatcccta ccatacttaa tatcaacact aatgagtaat
    11161 gaagatgtga gaatccgaat aaaaatgcca ttttcatttt gaaaaaatat cctttccaat
    11221 cataatgagc catgattggg aaaatataga gtgctgagag cacatatatg aacgcagcaa
    11281 tatacaaagc gatcctaaga taccaattgt gtatgaaaga aagatccaca agtaaaacaa
    11341 tcccgatggc gccatatatc cagctcatca gcaaggtttc acgatagttt tctttaaaat
    11401 atgtgaagta cgttttcgtc aaaggaaacg tttcttttga gcgcagccat tttctaatca
    11461 ctgcaaccat cgcatacgat gcaggaccga aagtaaaaac tcccaaacct gcgatgacta
    11521 aaccgcccca cagaaaattc aaataaacca actgtaaaat acgatttagc cttccgttta
    11581 ttttatctaa tgactgcagc atatctttcc ctctctttct atcttagtaa acgccgtctt
    11641 ctcctagctt tttggcaatc ttattagcta aaattaccat aaacaatcct acgacccctt
    11701 taaacaatcc cattgctgta ctgaaactta actgtccatt cttcaatcca gctgtataaa
    11761 tataggtgtc gaaaatttct gcaacgcttc gattcaatga attcaataac aagtacatat
    11821 gctcgaaccc taagtctaat gtctgaccga tttttagaat cagcaaggta atgattactg
    11881 gacgaattgc cggcaatgta acatgccatg ttttgcgtaa gcgtcctgct ccatccattt
    11941 ctgcagcttc atacaactgt gtatcgactg cagttatcgc tgaaaggtag atgatcgtag
    12001 accagcccaa ctctttccag atcacttgtc cgatatatac tgtgcgtgtc cattcagggg
    12061 atgttagaaa actgattttt tcaaatccca ttgttgcaag gaattcattc aatacccctc
    12121 cgtcaacatt caaaaataca taagtaatcg aaacaatgat tacccaagac atgaagtgag
    12181 ggatgtaaat gatcgtttga atcccgtttt tcaatcgttt gttcgttact tcatttaata
    12241 gtaaagacaa aatgattggc atcggaaaaa acacgaagat atttaatccg aacaagatca
    12301 acgtatttct taataatgta aagaatgtcg gttctgtaaa caaacgaatg aaatgtttga
    12361 aaccgaccca aggacttccc attattccta aaaatggttg ataatcttga aaagcaataa
    12421 cgagtccccc cataggaaga tacctaaaaa tcaagaaata aaggactccc ggcagaatca
    12481 tcacatataa aaatttattc gttttgattc ctgcaattat tttttttcgt cttctgagct
    12541 tttcttcttt tttcgatagc gtcagttgaa atggttcttg tttctttaca ctttccataa
    12601 gcccctcctt agttttgtga atgtttgctg cttatgactt catgatagat aactatctga
    12661 attacgtata taatcatttt taccgtaagt aaaaacgctt acatagcaat gatttttgtg
    12721 ctatacaaaa cgataaagtg tattttttca caaataaact acgcatagca aaccttctat
    12781 ttttttctat ctgacactta gcataggaaa tgattatata cgtaatcttt caccgatgat
    12841 aaattgaaca tatagaaaca gaaacattta atttattttg aggaggagcc tatctatatg
    12901 aaaaagaaag tctctatagg agaaagaagt tttacacttt tcaacaatac gtttctaatt
    12961 ttgctagctt tgatctgtat cgttcctttt ttaaatatca tcgctacatc ttttgcttct
    13021 acacaagaag tcgtcgcgaa aaagtttatc ctttttccta ctaccttttc tttagacgcg
    13081 tatcgctaca ttctttctac gccgaccatt tttagagcac ttgctgtttc gattggtgtt
    13141 acaggtgtcg gaacgatcgt cagtatgtgt gcaacttcac tgatggccta cggtctttcc
    13201 agaaagtatt tgtttggtcg aggtttcgtc aactttcttg ttgtattttc tatgttgttc
    13261 agcgggggga tgattccaac gttcttagtt gttcgttctc tagggctggt caattcttac
    13321 tgggccatga tcctgcctgt ggctgttaat gcaatgaata tgatcattat gagaaacttt
    13381 ttccaagcct tgccggatag tttggaagaa tcagcaaaaa tggatggttg cacagatttt
    13441 ggtgtatttt tcaaaattat gctgccatta gcgttaccgt ctattgctac tatctcgctt
    13501 ttttatgctg ttacttactg gaacacttat atgactgcta ttttgtatat caacgattca
    13561 tctaaatggc cgatacagat tcttttacgc caaatcgtca ttgtatcaag tgggatgcaa
    13621 gcagaaagta gtgccgttga tgtgattcca ccagcacaaa cagttaaaat ggctgttatc
    13681 gttattgcaa cagtgcctat gctgattgtt tatccttttg ttcagaaata ttttgtcaaa
    13741 ggagcactcg taggttctgt caaagggtga ttatgtactg aacgcaaaat ggtagttgtg
    13801 tcagaagtgg tcagcttcaa gaaataagaa ggcatttacg aaaattgctt cttaaatttt
    13861 tgtgaaattt cggcttattt ttcgaagaat ctactcctgg aacaccgtgt atcagaggtt
    13921 gtgaaagaga cgttcagcct tgagcgatga taaaaactct cacaaacatt gcgacgagta
    13981 acccacagga gcaataagaa gcgattttga aaaatagctt cccatatttc acaaaattat
    14041 gccttattgt cgatagcctg tctcttgaac gccatttatt cgtaaaaaag aactgtgaca
    14101 agacttttgt cacagttctt tttgcgtgct tagccttgaa atttaactat ccataaatac
    14161 aaatactagg gttattctaa cttcttttat tgcttgaatc agtccacaag aaaaacagcc
    14221 tcttgattca caaatatttt tttcgatatt ctcccggcgt aatcgattcc ttctttttaa
    14281 agaaacgaat aaaattttgc ggatttcgat attgcaatct ttctgcaatc tcttttacgg
    14341 agaggttagt ctctttcaac catttttttg ctacctctaa acgatgattt tgaacaaaat
    14401 caccaaattt ttctcctgtt tcctttttaa atacattact taaatagtta gggttataat
    14461 gcaacctatc agcgatcaca tctaaagaca gatcacgatc atattctgtg tagacgatat
    14521 gcataatatt ctccgacaaa tttttaaatt catgatcagt gattgcttga gagcattctg
    14581 tgattggcag aactaattgt tctaaaacaa gtttttctag ttctttggga tgataattgt
    14641 tcagtgcttt tacataaatt tgtttcatgt tatcaaagtt ttttgaatct actcccaata
    14701 actgtcctaa ttgaacgagt tcattgatca agcggaccga aactacttcc cgactcaaag
    14761 gatttttatt ttgtgcaaac aatgcatcaa tcaattcatg tactaaattc ttcacttcct
    14821 gatttccaga acgaatggct tcaaacaatc gattttgttc ttctatcgga tatctaataa
    14881 gtgttttttc atgagcagca ggaacgattt cctgataaaa aataattgaa cttggaccag
    14941 tatttacacg ataatacagg gcttcttttg cccgatcaac cgattgcttg ctttcattca
    15001 gcgtttcgaa ccggttactg atgcctatac tgatagttaa attcaagtat tcttttacac
    15061 gtttttgaat gagtcggcaa tactccatga cttttttgct tgcttctgca tcatctttat
    15121 ccattcgata aatagtggct tgcatttctt cattcaaaac gatcggcagc attcgatttt
    15181 cctttggcac aatttccgtg atcatgttgt tgatcgctaa aagtagcaaa tcacgctccc
    15241 caccgtgact atcttctaac agatcgattt gaaccaaagc tgtataatag cagtcatttt
    15301 gagaactata accaaattgc tgcaaccgct gatctagttc tcgcctttct actcgatttc
    15361 taaataaact aagtacaaat agcgtttcca gctgtggctt ttgggtaact aagtgtgcac
    15421 ttaacgcttc tttttctcct atgatccgac tgactgattg agcgaccaaa tctagttctt
    15481 tcccactgaa tccttttgat ttaagattca gacgttcctg tatttgagaa atcggacggg
    15541 caaatctgtc cgaaaataag taggaaagaa gaccaatgag tcctaccagt gttaaagaca
    15601 ctgcaactag cccgattctc agattgcgga tcattgcgcc aattgcacta gagtcaactt
    15661 ctaatctgta aatccaatta ttataatctg atttcagtac tatatacgtt ttatcttttt
    15721 ctcttatcac tttcagctga ttgtaatcta gatccacgtc cgttaataca gttggttgct
    15781 ctttattttt tactgtctga gtagtaaaaa ggttgccaac ttcgttttca atagaaagta
    15841 tgtcttctcc actttcacca acaacttgtt ctattgtatg tttctctatc gtagcaattc
    15901 ccaatgcata tttttctttt tcaaacattg gcaaagaaat caccatttcg attcctttgt
    15961 ttttcttttg ccaaaataaa ttatttttgt tatttatgta cttctgttga tacttttcaa
    16021 tttcctcttc ggtcagttga ctcaacgatc cattgattac tccccattta ccagccaaac
    16081 tgactaaatc ataattatta tcatccataa caatcgtttc tataaaattt agttctttcc
    16141 tgatttcaga ataagtggag taatctgtat atgtcagcgg atgcttgaat atgtccgaga
    16201 aatttttggt cgaaccatac gtagaaaacg aatattcaat cgttctaatt ttccattcaa
    16261 cgttagtttt aacttgcctg agcaaattct ttttgtctgt taaataactc gattcgatcg
    16321 attcataggt attctggtaa atgaataaac taaatcctat gacaagtacg gctcctaata
    16381 aaaacatcgg taaaaatatt ttataaaata ctgtactttt cttcattttc ttcctctcct
    16441 ccttttaaaa taaaaggctt tcatttttct gtaaaattga tactcatcta attaaatgag
    16501 cattccccgc tttcaagaat agcataatta aaaataaaaa tccaagtaat ttttaacgtg
    16561 tgcaatcttt tcatatcaaa gatctattga cttatcgtag ttatcgtaat atgatgaatg
    16621 cgttaacaaa aagtgaaaaa gtaaggaaaa tgatggaatg gtaacgatca aagatgtcgc
    16681 taaagcagct ggcgtttctc atacgacagt ttctcgtgct ttgaataaca gcagtttaat
    16741 aaaaaatgaa acaaaacaaa aaatacgaca actagctaag gaaatgaatt attcgccaaa
    16801 ctatagtgca aaaggtttag tgaatcaaaa aaaatatcta atcggactat ttttctcaag
    16861 tttgcataaa ggaacttcat caaatttcct agcagattcc attgcaggca tccattcagt
    16921 attagacaca aattacagtt tatctgttga aagtatcgat gttgttgatt taacagaaat
    16981 caacctgcaa cgctacgatg gactgatcgt tatgagtcaa agtgatgatg atcagccttt
    17041 catcgactat ctcaaagaaa aagaatttcc tttcgttgtt gtcaatcgtc acatcaatga
    17101 tccagcggtt atcaacgtca ctgccgatga tgcggccggt gttatgcagg cagtagatta
    17161 cggtattgga tgcggacata cgaagatcgc ttatattggc ggaaagaaaa attttcgttc
    17221 gacaaaagaa cgggaaaaag gggtattgaa aagtctaaaa aaagcgagca tccctgtcaa
    17281 tccagcctat tttttaacag gagattacag cttagaaagc ggcctccaaa acatggaata
    17341 cctattatca ctttctgacc tgcctactct ggttttttgc ggcaacgacg acatagcgat
    17401 tggtgcattg agagctgtac atttagctgg cctccgtgtt ccccagaaca tttctctgat
    17461 cgggttcgat gattctccag tcgtctccta tctcaatccg ccgcttacca ccgtacgtaa
    17521 gcctttgaaa gaaatgtgtc aagaaggaac aaagctttta ctagatttga tcgaaggcag
    17581 gacgatcgaa caatctaaaa ttgagctctc caccttgttg atccttcgtg agtcgatcaa
    17641 ttacttgtaa actacaagta attttatata aaaatgttaa cgtgtgcata acacagaaag
    17701 gaagtttata tgaaaaaact gacaaaagaa ttagtgaaaa aaaacgaagc acctgtaaaa
    17761 gttcttcagt ttggtgaagg caattttatg cgtggattta ttgattggca gattcagcag
    17821 ctgaataacc aacaattgtt taaaggtaat gttgcaatca ttcaaccttt agaacaaggt
    17881 cttggaaata tgatgaacca gcaagataat ttatatacgg tcattttaca aggactcagc
    17941 aatggcgaaa tcgttgatac ttccgagatt atcaccgtag tagaacaaat gatcaatcct
    18001 tatgatgaat gggatgctta tctatctcta gcagaaaatg acgacttggc ctatatcgtc
    18061 tccaatacaa cagaggcagg aatccaattc gtggagactg ataatttaac agatactccc
    18121 ccacagagcc tcccgggcaa actgacagcc tttttatacc gacgtttcaa attaaacaaa
    18181 gctggcttta ccatcattcc ttgcgaactg attgacagaa acggcgaacg tctaaaagaa
    18241 atcattttac gctacgcaga aaaatgggat ttagaacctg cttttgttga gtggttagaa
    18301 aaagaaaatg ttttctgttg tagtttagtt gatcgcatcg tcccaggtta tcctcgcgaa
    18361 gatgcaggtg ttttcaatca acggcataac tatgaagatc aattgatggt caaagcagaa
    18421 ccattcatgc tttgggtgat cgaagggcct aaagaattgg agaatcaact accattcaaa
    18481 caggctggct tgaatgtcat catcacagat gatatgactc cttatcgtga acgtaaggtg
    18541 catttgctta atggaccgca tacagcgatg gtccctcttg ctttgttggc ggaattggaa
    18601 actgtagaag aagtaatgaa agatccttta ttctccgcat atgttgatca gttattcaat
    18661 ctagaattga ttcctatgct ttctttacct aaagatgaat tagccattta tgctgaccaa
    18721 atcaaagaac gtttcttgaa tccttttgcc catcacaaat tagaagcgat ttcattaaat
    18781 agtgtttcaa agttcagcac ccgtctgctt ccagtgttca agaagtatat cgaagaacaa
    18841 aatcaggtac caccgttaat cactgtttca ctagctgcgc tacttcttat gtatcgaggc
    18901 gatcaagtaa agccacatga tgatgaaaag acgataagcg aatttactga tgcttggtct
    18961 gataatggaa cagctattcc tcgattattg caaaacgctg cgctttgggg agaagatttg
    19021 tctcaaatac cgaatgtgac agatacagtt caagaaatag ctgcaaagat cgatcaaaaa
    19081 ggtgtccggt cagtattaca agaaatgaaa ggaacgatgg tccatgcttg aaaccctgca
    19141 accaacaatg aaattaaatc gacgagacag tgtggtcgtc gcattaaccc ctattccaaa
    19201 acaaacacaa ctgacgattg atcagcaaac gatcaccact cttgaagata tcccgcaagg
    19261 tcataagatt gcattgggtg atttgaaagc tggagacaac gtgatcaaat atggctatcc
    19321 aatcggtcat gtgacgacag acgtaactgc cggtcaatgg ctgcacactc ataatgtaaa
    19381 aaccaatctt tctggtgaat tggattatcg atatgaaaaa gatgtccatc caagtcatta
    19441 cctttttgag aaccgaacat ttcaaggtta cttgcgaaaa aatggaaaag tagggattcg
    19501 taatgatttg tttttagtac ctaccgttgg gtgtgtgaat ggcattgctg aactgatcgt
    19561 caaacagttc aaggaaaaac atccagatct gggacagttt gaccatatta cgattttaaa
    19621 gcatccttat ggctgttcgc agcttggaaa agatcatcaa aatactcgag aaattttagc
    19681 ggacgcagta aatcatccca atgccggagg agttttagta tttggattag gttgtgagaa
    19741 caatacagtt cctgaattca aaaaaatttt aggtgaatat gatgaagaac gagtgaaatt
    19801 tctagtggct caagatgttt acgatgagat cgaacaaggt gtggctctat tagaagagct
    19861 tttaacagcg gcggaaaatg accagcgcat atcagtcccg ctctctaagt tgaatgtcgg
    19921 tttgaaatgc ggcggttctg atggtctatc gggaataact gctaatcctt tacttggtgc
    19981 attttctgac tacttgattg ctcaaggagg cagcacgatc ttaacagaag tgccagaaat
    20041 gtttggcgca gaacaagtat tgatggcacg agcggaaaat gaagaagttt ttgactcaat
    20101 cgttgacttg atcaatgact tcaaacagta tttcttgtct tacggggaac cagtttacga
    20161 aaacccatct cctggtaata aagcaggtgg gatcactaca ttagaagata agtctttagg
    20221 ctgcacgcaa aaatcaggaa gctctcctgt cgttgatgtg ctacaatatg gcgaaaaaat
    20281 cagaaaacca gggcttagtt tattgcaagc tcccgggaat gatcttgtag ctgcctctgc
    20341 tcttgcttca tccgattgcc agttggtctt gtttaccaca ggaagaggga ctccgtttgg
    20401 ctcatatgtc ccaacggtca aggtatctac gaatacaact ctttttgaac gcaaaggtca
    20461 ttggatggat tttaatgcag gcgtcttatt agaacaacca atggaagtaa tcttagaatc
    20521 gtttgttcaa aaaatcatcg ctgtagctag tggagaagaa acgaaaaatg aacaaaatga
    20581 cgttcgagaa attgccattt ttaaaaacgg agtaacactt tgattttcat aaaacagtat
    20641 aaaaagaaag aaggaagatt tatgttttta tctgatgatt ttttattaaa agacgattgg
    20701 gcgaaaaagc tttaccattc ctacgcaaaa aacatgccca ttattgatta tcactgtcac
    20761 ttagatccaa aagaaattta cgaaaacaac aatttcagca atctgacaga agcttggtta
    20821 ggaggcgatc attacaaatg gcgcttgatg cgagcttgcg gcgtgcctga agaaaaaatc
    20881 acaggaaatg cttctgattt tgaaaaattt ttagcttggt gtcaaaccgt accaaaaatt
    20941 atcggaaatc cgctttacag ttggtcacat cttgaattga agcgtttctt cggtattgac
    21001 ttgttgataa atgaagaaaa tgcagagaaa atttggacac aagcaaatga aattttggca
    21061 gctcctgatt ttcgccgtcg ggaaatcgca aaaaaatcga atgtagaagt gatttgtaca
    21121 acagatgatc ccgcagacga cttgcactat catcagttgc ttgctcatga agaaccagat
    21181 ttgaaggttc taccttcatt ccgtccagat aaagcactca atattgaaaa agaaggattt
    21241 aatgcatggg tcacactact ggaagaagca gctgaccaaa cgatcaccac ctatcaagaa
    21301 ctgatagaag tcttagggca gcggatagaa tacttccatc aacatggatg tcgtgtttcc
    21361 gaccacgctt tagacatctt acgctataaa gaagcggatg aaacagtatt agaagagatt
    21421 tttcaaaaag cacgagctaa caaactatta tcttcagatg aaatcgatgc ttatcgtaca
    21481 gaaacactga tacatttgac ccacttctat cattctcata attggacgat gcagctgcac
    21541 atccatgctt atcgtaactg taatacacaa atgttggaaa aattagggcc cgataccggc
    21601 tatgatggaa tgaatgatct ttctttgact attcctctgc aaaaattatt gaaccgtgca
    21661 gaagaaacgg atcagttgcc aaagacgatc ctttattcat tgaatccgaa tgactatcct
    21721 gcgattttag ctttgatggg ctgtttccaa aaagaacaga atggaaaact gcagttaggt
    21781 tctggatggt ggtacaacga tactcgagct ggtatgcgcg atcaattgac acaatttgct
    21841 gatggtagtg ctttggggaa ttttgtcggc atgctgacag attcaagaag tttcctgtcc
    21901 tacactagac atgaatattt ccgtcgtgtt ctttgtgaat ttttaggcga aatggtcatg
    21961 cgaggagaag cacctgaaga tgaacaactt ttaggaagtc tagttcaagc gattagttat
    22021 acaaatgcta aaaattactt tggctttttt gcctaattga ttaatagtaa cagtaacgaa
    22081 tcaaaaacag gatagtgaaa taacggaatc cgtttatcca cgttttagag aagaagacgg
    22141 accaaatttg ctccgtcttc ttctcttgct gttctgcttc attgcctata aacctttatg
    22201 aaagaagaga taacatgcct actatccata ttgccggtga ttctactgcg gcagaaaaag
    22261 atgaaaacaa gcgtcccgag acaggttggg gagaaaaaat ctctgcttat ttttcacccg
    22321 agatccagat caataaccaa gcaaagaatg gacgcagcac caaatcattt atccaggaag
    22381 gaaggttagc ccgtctagct tcacagtttc agccgaatga tttcttattt gtacaatttg
    22441 gacataacga ccagaaaatc caagaagaaa gaggtacgct tcccttcggt agctatttaa
    22501 aaaatctgca gatatttttt gaatgcgcaa aaaaagcgaa cgtccaaatg gtcttactga
    22561 catctgtgac gcgacgagat tacttagaaa acggaacatt gaatccagat atacttggag
    22621 actatccaaa agcgatgcgt gcatttgctg agaaacacca tattcctctt ttagatgtgt
    22681 tctcaagatc acaagaattg ttccagacct tctcaaaaga agagacaaaa aaattctacc
    22741 tgcatcttat acctgatact tgtaaaaatt acccagaagg actaaaagac aatacccact
    22801 ttagtccaga aggcgctgat aaagttgccc gaataattgt tgaattgata aaagaatccc
    22861 gtctgcctct tgctaattat ttacaaaaag gagtttgaga tgtttccatt aaatttaggc
    22921 atccgtgccc acgatttaga tgttcgaaat cgtgaagaaa tcgttcaaaa agtatcacag
    22981 cttcacttgt cccatatcca atttgcacca caaaaagcat ttcctgaaca acttaaatta
    23041 actgacatga cacttgggag tgctgcctat tttggtgaat acttccaaaa aaacaatatc
    23101 gaactttcca ttttaggatg ctatatcaac ctatccagca aaaatcaaga agttcgtgca
    23161 caagcattgg caactttcaa acagcaaatt gctctgtgct ctgcctatca agccaagatg
    23221 attgctacgg aaaccggtag tgtctctgtc ggttacacaa aagaaaattt tactgaagaa
    23281 gcttatcaga tcgccagaaa ttctatcatt gaattagtcg ctcatgcaga aaattttggc
    23341 gtaactgttg ctgttgaagc agggatcaat catccaatct ataactatca gttagctaaa
    23401 cgtttaatcg atgaagtagc ttcaccaaat ttaaaaatca ttttagattg tgcgaatttg
    23461 atgcataaag aaaatcatgc tgatcaggaa aaagtgatcc gaggtgcctt ggacaattta
    23521 catggttata ttacagcgct tcatctcaaa gactatatca tggaaaatgg aaagatccgc
    23581 atggtacctg ttggcaaggg ctgtttagat ttccgcccgg tactgcatta tatcaaatat
    23641 gagcgtcctc taatgtacgc cactcttgaa gctactcctg agatttatgt tcctgaagca
    23701 atagaacact tgcagtttat ttaccaagat tgttaaggag aagattatgg aggaacgaat
    23761 acagaaaagt tatcaaaaat atcatgcaaa tgcttttcag acattgctgc atctttatca
    23821 gggaaattat tggcgttttg ccatgtcagg actctttttt attatcaaac atcttccatc
    23881 ttggttaatg ccgatcgcta ttgccaatgt aatcaatgca gcttcatcga aagattcttc
    23941 tcagttgcag gccatttatt tcaacgcttt tttgatggct tgtctgattg gtcaaaatat
    24001 attgacgaat tattttcatg taaaatacca tagcacttcg atccgtcaag tagaagctgg
    24061 actaagagcg acccttatca agaaaatcca agaactatct attccttatc aaaaagaatt
    24121 gcaatctgga cgaatccagt caaagatcat ccgtgatgta gaagctatcc aaacgttgtc
    24181 ctctcaagta tttgtcagcg gattaaatat cgctgtcaat ttattcgtag ctttaggtat
    24241 cacgctttat aaaagtcctg ttgttttcat gtttttctta ttgactgttc ctctcgccgc
    24301 attcgttact ctctttttcc gtaaaaggat cagacagtcc aactcagact tcaggcaaga
    24361 aatggaagag acttctgcaa aagtgatcga agtgatcgaa ttagcaccga ttgctcgagc
    24421 tcatgggtta gaaaaacacg aaacaactcg cttaaataag cattttactc aaatctatga
    24481 aaaaggattc cacttagata tcctgcaatc tgttttcgga tcagtcagct gggctagttt
    24541 tcagatattt cagctgattt gtttgatttt cactgggata ttagctttac aagggcgcat
    24601 ccaacccggc gatgtcgtga tgtatcagac gtatttcacg acgatcgtca attccgtttc
    24661 tggaatgatc acgctgattc ctacgatttc aaaaggcatg gaatctatca cttctattgg
    24721 cgaactgttg attgctggcg atgtagaaac ttatcacggt aagaaaaaaa tccaacattt
    24781 ggatggaaat tttcgttttg aggatgtttc ttttctttat ccggactcac tcaaaccgat
    24841 tatcgatcat ttttctctag aggtgaaagc tggagaaaca attgcttttg ttgggccatc
    24901 tggctctgga aaatctacac tccttaactt attgattgga tttatccagc cgacttcagg
    24961 aaaaattttt ctagatgatc agcctttgga tgaactggat atgcgaagtg tgcgaaatta
    25021 tttagccgtt gttcctcaaa ccactttgtt gttctccgct tcgatcaaag agaacatcac
    25081 ttatggcttg aaaaacgtat caaaagaacg tttagatgaa gtcatcgaag ctgcgcaact
    25141 ttcttcactg attgaccagc ttcctgatgg tctggatacc ttagtaggcg aacatggcaa
    25201 caagctgtct ggtggccaaa agcaacgtat ctccattgca cgagcgttga tacgtcagcc
    25261 aaaaatcatt ttgctagatg aagcaacctc agctttagat aaccagtcag agaaaaaaat
    25321 ccagcaagcc ctgaattacc tgacagaaac acccactact tttattgtgg ctcatcggct
    25381 ttcaacgatc aaagaggcag ataaaatcgt tgtgatcgat gaaggaaaaa tcgttgaaat
    25441 aggtacttac gaaaaactgt tagctcaaaa aggctatttc taccgcttga aaaatgaatg
    25501 acaaaaaata ggagctatga caagactttt gtcgtagctc ctatttctga ataaacggtg
    25561 ttctcctgcc aactcctcgg tcacaagtca caatagtcat caaacatgag aagctgtttt
    25621 ttgaaattgt tccttgtaac tcagagttaa acggtaggct cacgacctct tggtaaatgg
    25681 tgttcaagag acagcctatc ggcaataaga agcaattttg tcatcgctca aggccgaacg
    25741 tctctttcac accctctgtt gtttcataaa atagttttat tcgctgtaaa ggtcgcgaac
    25801 tggtgtcatg atgatacggt tcaagtcatt gatgattgta ctgaagcctt gctctttttg
    25861 catcaagtca ttgattactt cttctgattg gattttcaca gccatttctt gtgctttcat
    25921 tgcatcttcg tcgctgaact cttgtccact catttgtttt tcttgtaatt cgatttgcat
    25981 cgcttggaag tctttgaaca gtttgtgtgc ttcttcgttt gcttgtactt tctcgtacgc
    26041 ttctttcaac gctttgaatt ctggtaattc acggatctca cgttcgattt gattcgcgct
    26101 atcgtagatg tttgacatgt tatgccctct tttcttttag tagttactct ctattgtaac
    26161 atggggttat tcgttttcaa aggggctact ctccttgata tttccgccag aggtctttag
    26221 ccgcttcacc aattccctga acgccttctt tcaccatatc gccgaagttt ttcagccctt
    26281 cttttgcttg ttcaccatat tgattcaagt tatcttgcca atttccagac tctccattag
    26341 aatttcctgt gtttccaact tgatctgcag ctaccacttg tcctcctgtg gcataggcat
    26401 ctgctacact aaatcctgtt tttgaagtat acggaaggat gccttctgcc gctgatttga
    26461 agactttccc gacaacattt ccacttgtcc cttccaaata gtgcagcttg ctggattctt
    26521 cgtaacccat ccatgtcgaa atcactacgt ctggcgtgta accgatcatc cattggtcat
    26581 ttgcttttgt ggcatcgaaa ttagtttctg tggttcctgt tttccctgct atcgtataac
    26641 ctgcaggatt cgcagcagcc gctgttccat ttgaaaatgt accaagaagc atgctggtca
    26701 tttcatcggc tgtttccttt gatatgacct gttttggttt tttgtttgtg ttatcgacga
    26761 tcaccgctcc agtcgaatca acgattttcg tgataagatg cggcgtatac atctgaccat
    26821 cattggcaaa tgcactgtaa gcacccgcca tgatcaatgg agatactcct ttttccagtc
    26881 caccaagggc taagccataa tacttatcag attttgctaa aggcaggccg aattcttctg
    26941 ctttatcata tcctttttgt aaaccgatct catgcaaaag ccatacagta ggcaaattca
    27001 aactttgtgc aacagcttga tacatcggta cttctccttg gtatgtcccg tcaaaattat
    27061 gcgcatcgta ataactttgc ggttcgtctt tcaagatact ggaaggcttg tatccagctt
    27121 ccagtgctgg tgtatatacg cttaatggtt tgatcgttga tcctggtgaa cgacgcatct
    27181 gtgtagcaaa gctgaatcct ctaaagacat gatctcctct tcgtcctact agtgcttgta
    27241 caccacctgt attcggatct aaagctactg aagcagattg gaccatggca ccatctgaag
    27301 catttgctgg gaataatgca ctgttttgat aagtagcttc taattgcttt tgataatcct
    27361 gatctaaagc tgtatagatt ttatagcctt tattcatgac atcttcttca tcaagaccat
    27421 actcgttaac tgcttcatcg atcactgcat caaaataata aggataacga taaccagaat
    27481 ctgaattatc gtatgtgtca ttaagatacg cgctgatatc tgtctggctt tctgcttgtt
    27541 cttgttcttt tgtgattttt ccgttttctt ccattacact tagtactgtg tttttccgat
    27601 cattcgcatt ttttgggtga tcgatcggat tataaatacc aggacctttc agcattcctg
    27661 ccaaagtagc agcctcagac agcgtaacat tcgttgcatc tactccaaaa tactttctgg
    27721 aagcgtcttg gacaccccat accccgttgc caaaataggc attgttaaga tacatggtca
    27781 agatttcttc tttgctgtac ttcttttcga tctcgattgc caaaaataat tccttcgctt
    27841 ttcgatcaaa cgtttggtct aatgtcagat acgcattttt ggcaagctgc tgagtgatcg
    27901 tacttcctcc gccccctgat gttccgccag acaagaccat tcggactgct gcacgggcaa
    27961 tccctttgat atcaaacccg tgatgctcgt aaaaattcct atcttctgtc gagattactg
    28021 catcttggat atagggagaa atcgcgttta attctacata tgttcctttt tgggcgtaca
    28081 gtgtgccagc ttcttcgttt ttcttatcat aaatgactgt cgattggctt aatcctgatt
    28141 ttaacgtttc gacatttgct tgttttgcta aaataaagag gtagatactt gtcactaaga
    28201 cacagatcaa accaatcagc aaaatcaatt tgttcacatg atattttttc cagatgcgct
    28261 ttcgccatcg atggaattgt ccgagatatg gtctgatcca acgccaaaaa ctgatcaaag
    28321 cagcttttat cttttctaaa atgctttgaa aatccatttg cttcttctcc tgtctatttg
    28381 attcaatcgt cttattttac catagtgaaa gagtgttttc ttcctgctta agttgtagtt
    28441 caatatgttt ttttacgtta gtgcaagtag agtaacataa gaatgaacag aaaaatgtaa
    28501 gttttttctt aaatatgttt taaaaatggt acaattagtc tgcgattgca ctgaaaggag
    28561 tacacttgtg aaaatcacga tcactttacc tatgaaccat cctgaaacaa caattcgcga
    28621 cttgcttgaa aaggaatggt tagttcctag aaaggttcga cactttttgc gtatacgtaa
    28681 aaacgtttgg atcaatcaac aacctgcttt gttccatcat gaagtaaagg gcaatgacca
    28741 gatcacattg ttattcgaag aaacagatta taattatcaa gaagttcaat taggagatgc
    28801 gaagcacatc cgtgttttat atgaagatga gcatttgatt atagtggata agcctgcagg
    28861 aatgaagact catccaaatg aaccgacaga aaacagtacc ttactcaatc atttagctgc
    28921 ttatctcgac actaaacagc aacgacctta cgttgtccac cgcttggaca aagaaacaag
    28981 cggtgccgtt ttatttgcaa aaaatccatt tgtcttgccg atacttggaa gaatgttgga
    29041 acaaaaaatg atctatcgga gatatcaagc catcgtatgg ggagaaattg ccaaagatat
    29101 gaccatcacc aataaaattg gacgagatcg ccatgatcgg agaaaaaggg tcgtagatga
    29161 taaaaatggt cagagcgcct tgacacatgt cactgtcaac aaagtacttt ctgaacaaac
    29221 acaagtctat tgtgttttgg aaaccggacg tacacaccag atcagagttc atttagctca
    29281 taagggacat ccgatcgttg gagaccctct ctaccaaaat agaacagctg aacgactgat
    29341 gctccatgca ttagaactgc acatgatcca tccatttact caagaaaaaa tagtcggtaa
    29401 agctttacct ggattatggg aatagaaata aaaaagaaca gagtcttatc agcagataat
    29461 gataagactc tgttcttttt gtgtaatttg tctgcttact tttgatgttc gtgttctgct
    29521 aacagcaaac taccgataat cccactttca ttcggcagtc cccaacgaac gatatattca
    29581 cttacttgag gtgtttccat ataaccaccc atcaattcaa caaattgctg acggattttt
    29641 tgaaggagat gatcctgatt cattacccca ccgcctaaaa taatcttttc aggtgctaac
    29701 gtcaacgtat agttgatcag tgcttgagca atataataag cctgaatagc ccaaacagga
    29761 tgatcttgcg gcagatcttg ccctttgatc cctgtgcgtg cctcaataga cggtcctgct
    29821 gctaatcctt ctaaacaatc tttatgataa ggacaagtac cttcataatg gtcatctgga
    29881 tggcgtttca cccaaatatg tcccatttca ggatgtgcga tccctgaaaa aatttcttca
    29941 ttgatcacga cacctgcacc gattcctgtt cccacggtaa ggtaaataca actatttttt
    30001 ccttgtgctg ctccttgctt caattcccca tatgcagcag cattgacatc cgttgtccaa
    30061 gcagcaggaa tattgtagcg ttccttgatt gtgccaagaa aatcgaattg tgcccaccct
    30121 tttttgggag ttgccaaaac gtatccgtaa ttatcttttg ttttgtcgat accaatcggg
    30181 ccgaatgagc caatcccgat tcccttcaat tcataacgat caaaaaattc aaatacctga
    30241 tccaatgttt cttctggtgt tgtggtaggg atacttaatt tttctaaaat ttctccacca
    30301 tctgaaaccg cacacacaaa ttttgttccg cctgcttcta ttccgccata cattcgtttt
    30361 cctcctcgaa tccgattgtc cgaatctcat aaggttggat tattgggaca accgcttgct
    30421 tcgttggttc ttcaagcaga ttcaatatga cagtttttcc attgtcttta gttatttcca
    30481 atttttctaa atgactagac atattgaaac ctcttactac tactttatca ctaaacttgc
    30541 tgcgtttcaa agctgtaatt gcaaacgttt cactttttat cgttaaatat tgttgttttg
    30601 aaatcaatgt tccttcatga tgtttgattt gttgagtgga aaaaggaatc tgtgctgcgt
    30661 atgcatgcaa ataagtagag aatttttctt ctggtccatg tagttcaatg ctgtaattga
    30721 accgatgctc acctagacac tgtgcttctg gtgttggaaa atatccccaa tcgcctagtt
    30781 ctcccacact gcgaagtagc gtcactgcga tttgtccgtc ttctgttact tcatattcat
    30841 tcaaaccaaa gtttccgact gttacaccgt actcttcatc atgaatattg acaaatgctt
    30901 gctgatgttg cggattcgtg gggttttccc aacttttaga cacatggttt gggcgcttca
    30961 ccacttcaaa aatactatct gcttcgtgat gttccacatg taactttgta ggaaacaaga
    31021 cacgtaagcg atgatctttc atctgattgt cgagagtcgt ttcaaagtcg atttttttac
    31081 tatcttttct gattgtcatg attgttttta tttgaaggat tcgtttttct ttggatcgtt
    31141 ctgcttttcg ataacgaaat tccacaacca tctgctgttc tttttctaaa cgctcgtctg
    31201 cagaaacagg gatttccatt tcctgtagaa tacttatttt tgctatttcc ggtgtatcta
    31261 ctaatacttt tacttcagag ttttctttgt tactggatag gattggctga tcacagaaag
    31321 gttgtttaaa gatatactca tttcctatat cgccgatatc ttcaaaaata tgcagattat
    31381 tcaactttcg tttgtttttg cgatcttcca ttgtaatggt accatttttt tcaatcgtca
    31441 aatgtagagg gccgttttcg atgattcgtc cactctgatg aatcattgtt tccttttctt
    31501 ctgctttcgc ttccccttct acaagcgcga atgtttccca agaaaatgct ggcatgtttt
    31561 ctaatggtaa agtcactttg atcattcttg ccatatatgg cacacggaaa cggtctttcg
    31621 gcaaatcata accaaattgg acagtttctt cagataaaat agctggtata gtggccccac
    31681 tttttgtttc tacatgatac ttacgtttag gcaaattttc taactcttga tatagttgtt
    31741 caggtattcc ttcagcgaat ctttttcttt ctaattcgat ggttatttct gcttcaccag
    31801 ttttatccat tcctgcagta tttacaatga caaatggaaa gctgtctttt gggaatccgg
    31861 tcgtatcgat tgctgcggtc agctgttcta aggaatcttg tgcaagatac tttccaactt
    31921 catctgcttt ttcaaaacga ggtatcattt cccgatggac agaatcgact gaacaaccgc
    31981 aaatactgtc gtgaggatga ttttgcatta atgtcttcca agcataatct aattgatcat
    32041 ggggatagtt tccactaact tcataagcca tcgtcgcaag tggttctgta atattttcta
    32101 gctgtcgctg gaccttggta ttccattgtt ttaagtaaac ccgagcagat gctgtattgg
    32161 ctaaagtata ccagccatcc gtttcttgac ttgtcaattc tccttctact gagcctaagt
    32221 cttctggcac atctttttga actgcttcca gatagtcagt aaaattagaa tgaataaact
    32281 cgtaatctgg aaacagttca tttgcgagat ggattgcttt tgatatatct ttttgtaccg
    32341 gttgatgatc aacaccattc atcatcaata agtggttcgt agaggcatat tgttctgcat
    32401 ccgctaattt ttgcttccaa aaggcaagag ctgcttcttt ttctactgga atctcattcc
    32461 cgttgctata ccaattggca aatagcagac caaagattgc tgtttgatca ggtcctttcc
    32521 accacatctc tgagtattgg gaagaatagt tttctgcttc caaaacttga ttatcaaagc
    32581 caattggttt tacaccacgg ccaaacgctg ctgctgaaat accagcttgt ttcattaatt
    32641 gaggcgtctg tcccatattt ccaaatgtat cgggaaaata gcctaacatg acaggggttc
    32701 cccatttacg gctttcttcc atgccaatca gcatatttct cacgttcgat tccgagctga
    32761 taagaaaatc atcttgcaag atataaaatg gtccaattct caattttcct tcattgattg
    32821 cttgttggat tgcctgtctt ttttctggcc ttacttgaag atagtcatct aaaataatcg
    32881 tttgcccatc caaatgaaaa ctgttgaagg acggatcaac ttcaaatagc tctagtagat
    32941 cgtctatcaa ttcaatcagt cgcatgtgat gctgttcgta agccatgtac cattcacgat
    33001 cccagtggct atgagaaatg atatatactt tctttttcat ttacgatacc tcctactttt
    33061 ctactctaat atcaaagtaa tccatgacta attcgcaaaa catcatattt gcccaagaaa
    33121 accattctct cgtataattt tcaggattgt tcacatcgaa tccttcgtgc ataaggtgtg
    33181 ttcctgcgtc agttgctaca agtaaattca atatccgctc tttttcagat ttgtctgacg
    33241 ttgtcattcc ttccatagct aatgcaatcg gccaaacgta tttttctggc gtatgggagg
    33301 atccaattcc ttttgcgtac tttccttcat agaaatatgg attttcttta ctcaacaatg
    33361 ttcttcttgt ttgcagataa tcttcctctt gttcagaaca atatcccaag taaggtgcgg
    33421 cgatcagatt gggcacatta ctgtcatcca tgactgtggc gtttcccatt ccatctactt
    33481 catatgcata aatcgtttgc cctttttgat taaccgttcg tccgtgattc tgaatcccct
    33541 cttggatctc tttcttcaat ttctgtgttt gctgtacaat ttctttatca tcgagtatcg
    33601 tttgaaaaat ttcttccaca taacctaaca cgaccaccgc aaacatattg gatggtacca
    33661 gatatccata acggcaagta tcatcgctag gacggaaacc agaccatgtc attcctgtaa
    33721 aagctgtttt tgcacctcgc ccgtcattga tcagcgtatc ttcttttctt gtcgtgtctc
    33781 gtacaaatgt ataaggagag tgttcatgat cttgctctgt tttgaacacg tccaagattt
    33841 ttttgatacc ttcaataaaa gattgattga attgatcagt ttttcctgta tttttgtaaa
    33901 gaagataggc caactgtatt gggtagcata aagaatcaat ttcatatttt ctttcccaga
    33961 tccaatcgtt catttttgta tgatctgttt ggtggccagc accgttagct tcttcgttga
    34021 aggcattcgc atatggatcg atattgatat aaaaaaattg ctttttgact aacccgctga
    34081 tcatgtcagc taattcctca tcttcttttg caatcactaa ataaggacga acttgtgcag
    34141 tagagtctct taaccacatc gctggaatgt ctcccgtcaa taaaaaggtg gttccgtcat
    34201 tttgcttttt gactgttgtc aacagagtat tggcaaatgc tgctgtaaaa ttctttgccc
    34261 agtccgcatg gatatcccca catttttgtg tgatatcttc cataaatttc tgcaccgatt
    34321 ttggtatatc tttataagcc actaataaaa ccttctttct atttgatata tctttaaaga
    34381 ttatacttca tacagccaat tatgtctacg ctttcaaaaa atatcttttt atttactttt
    34441 tcatccatta tggtattatt tgaatatgat atatcattaa gaggtgttta tatgaatcaa
    34501 ccattgtacc agacaatatt taatgatttg caaacggcta ttttaactgg tgaattacct
    34561 gtagacagcc aactaccaac tgaaaaagaa ctttcacgta cctataaagt aagcagaatc
    34621 acttccaaac gtgcattgac tgaattagag caattaggac ttatttatcg cgttcgcggg
    34681 aaaggcagtt tcgttaaatc cgcttctccg gaacagattc ttgcttctcc tacaaaaaca
    34741 cgacggattc tgtttcttct tccatatgtc gctgatttgt ctgttggtga ttttagcaag
    34801 ggattgaacc ctgtgatgca agataaacag attgatgtca tgatgacgac gcttgacttt
    34861 ctacaaaata aaacagcaaa agagctaatc aatgaatttg acggattgat ttattatgct
    34921 cttgatacag aacaacatct tgacttactt tttgagcttt cattgaaaga attccctgtc
    34981 gtcatattag ataaaaaaat ctatgagtta ccttttccta ccgttctttc cgataatttc
    35041 caaggcggtg cgcttgctgc ctcttatttg atcgaacagg gacattcaaa aatcgcttat
    35101 ttgtttggta cgcagactca tccacaatcc gttcgacagc gttaccttgg ttaccttgaa
    35161 gcattggaca aagctggttt gactttccac acttcactgg atgacaaaag cgcgacatta
    35221 gatgaattgc tttcttatat cactaagtat gaagtaactg cttttgtttg tgaaaacgat
    35281 gttgtcgcta tccagaccat gaatctcttg agacagcatg gttatcatat ccctgaagat
    35341 ttttcaatta tcggatttga tgatatccaa gcagcagctc ttgttgaccc gccactgaca
    35401 accatcgcac aagattttga acagttaggt aagcttgcgg gagatgcttt ggtcacttgg
    35461 ttagagacaa aagatcgacc atccgatagt aaatttccag tttcattcat caaaagacaa
    35521 tcaacaaagg agaaataaaa tgaatatcgg tcaaatagat actagacatg caacagcgaa
    35581 tcagccaaat ttctctaatg ggaactgcca gccttatact ggcgttcctt ttgggatgaa
    35641 ttattttgca ccgcaaacta cggatcaaaa cggcagctgg tggtttcatc ctgatgaccg
    35701 cgtttttcaa ggctaccgtc ttacccatca accaagtcct tggatgggtg atttcagtca
    35761 tatgctgttt acgccgatca atggaaaact acaggagaac acccttttcc atgctcaaag
    35821 ttcttaccgc ccagaagaaa gtatattttg cccgactcat ctgtcgatct gtcaattacg
    35881 ttatggcatc tgttctacat tgatacctag catgtatggc ggaatcttat ctattgatta
    35941 cagcaaaaat gacagcggtt tacttttatc ctttccggga cgccatcagc tctttgttat
    36001 agatccttac acagtggaag gaaaaatcac acattttgct ggttcagaag atcctgattt
    36061 ttctttttat tttatattga gatttgaata tcctttaaca attgatgagt tagctgtttc
    36121 caacaaaaat agcgactcga tcccgttgta ttttgaacag acaaaaaaac aaactatccg
    36181 atttgggaca tcttttatca gctcggaaca agcagcttat aacctggaac tagaacaaaa
    36241 ccaaagcaaa gagacttacc ttgctcacag tcaagaacaa tggcagtcct attttgatcg
    36301 tatacagctc acacaccatg atcaacaaca attgcggacg ttttatcata atttatatcg
    36361 ggtgttttta tttccacaaa cattttatga aattgatcct agtgggcaaa aaatccacta
    36421 tgatacgact agtcgatcta taaaacctgg tgttctctat acaaataatg ggttttggga
    36481 tacgtataaa acggtttatc ctctttattc tttgattgct gtagaaaaat acgaagaaat
    36541 gctggaaggt tttttgaaca gctatagaga aagtggctat ttgccaaaat ggctgtctcc
    36601 tgatgaacgt ggacttatgc ctgggacatt gatcgatgcg gtcattgcag atgctgctgt
    36661 aaaagatatc cgacgagact tgatgcctga atttctaacg gcaatggaaa aaggtgctac
    36721 ggttcaaagc aaacatccaa attatggacg ccaaggaaca gaagattact tgacatatgg
    36781 ctatgttcct aatcactacc acgaatcagt caatcacaca ttagactatg cttatagcga
    36841 tttttgtatt agccaagttg ccaaggtact agaagaagag gaaatgacga agaaatatca
    36901 gcagcaatca ttgaactatc aaaatatctt tgactatgag tcaggattta tgcgttcaaa
    36961 agataaagat ggcaagttca gagaaccatt ttcagctaat cgttggggac aagattatgc
    37021 agaaggaagt gcttggcaaa gcagctttgc tgtttatcac gatttttcag gactgatcgg
    37081 aaaatatggt ggcagtgaga gatataaaga aaaactcatt gagctgtgca atcagccccc
    37141 aacgtttgac ccaggaggat atggttttga gatccacgaa atgagtgaga tggccgcgat
    37201 agaatttgga caattagcta tttcaaacca gccaagtttt cactatcctt acttgttcag
    37261 ttatatagga caacctgaaa tggcacagcc actgcttaaa cagttgatga ctcagacctt
    37321 cgacgattct ccaatgggtt accctggaga tgaggacaat ggaagtatgg ccgcttggta
    37381 tatcttcaat agtctgggat tttatcctgt cacgcctgga accggtgaat atgttatcgg
    37441 tatgccatta attgacaagg ctgttttgct tttgtcaaat ggaaaggaat tgacgattga
    37501 agcaacgcct aatcggccgc agcaacaatt tatccataaa atcaaacgga tggacaaaga
    37561 atatacgaaa ttatatttta cccatcaaga tttgctgaaa ggcggcacga tctcttacca
    37621 attagggatc atccctcacc ctaagaaata tcaagcaaaa gatttgcctt tttccttatc
    37681 aaactataat taacaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc
    37741 aagcaaaaat agatcctcat atttttcgtt tggttggtct tattaccgca gatgcaagct
    37801 tttgaacacc gtttattgag aggttgtgaa atcagcgttc tgacctgagt attagagaga
    37861 caaatggcaa aatagttcct catattttgc cattttttga gctaataccg agggtcagct
    37921 gattgaacac cgtttagtgg agaggtcgtg aaaaagctgt ttagtttcga gtattaagaa
    37981 aaattttgtg aaaatggctt tccacatttt tgcataaatt tggcttaata ccgagaaact
    38041 ggcttttgaa ctccgtttat cgagaggtca tgaaaaagct gccttgcctc aagtaataag
    38101 aacagcccaa aattagattt ctagacaatt ttttataagt tatttcatta cattggcttt
    38161 ttcttttatg attctccact gttttctcgg tactctttag gggatctgcc gacgattttc
    38221 ttgaataatt tagagaaata cgtggtgttg ttgtagccaa tctcacttgc tatctcattg
    38281 atgttttgat tactgtgcaa aagcagttgt ttcgcttttt ccatacgtaa atggttcaaa
    38341 taagctgaaa aacttttttt cgtttctttt ttgaacagct gtcctagata catgacattt
    38401 aaatgcaggt tttctgacac ttctttgagt gtaagtggtt cttgataatg ctggtgcagg
    38461 attacgatta ctttttccac attgtcactg tactttttct gtctctgatg ctttttgatg
    38521 aatgacagaa gtagctgctg aagttcttgc accgtttttg cctgatggat tttttcgatt
    38581 ccttgtaaat attcctcatc ttccagatca atcaattccc gttgaatctc cataaacagt
    38641 aaaaaactgg aatatcgtat atcttccggc atcatggctg ctaactgaaa ctgttcaaaa
    38701 aaatcagtga tcattttttg agcgacttcc aattgtttat tttgcagcac gcgtctgaaa
    38761 cttgaaaaat cgatggtttg atcttttgtt tctgcttgta ctgcgtaaaa aacatgctcg
    38821 tctttatctc cataaaattg atgcaactgt agattgtctt tcgcttgttg gaatgtttca
    38881 gggattttgt ccaaagaatc tgtctcttcc ccgatactga tcagccattc ctgatcagga
    38941 aagttttctt gaacaaaaca acagaagcca tcaacttcat ctgcctccaa caacaacgtg
    39001 atcaatacac ggtctccgta atttcgttga tagtaaaaga tttgttgccg ttcttgcaac
    39061 catctattga taagagtatc atgagttctt gccagttgaa tcaatactat cctatgctgc
    39121 ttctcactta tttgtcctat tatttcttct tcacttattt catccaattc ttcattcaac
    39181 cattgactga ataacagttc ttgatagatt tcttgttgat ttttttgttc attttgctga
    39241 tctaaccgtg ttttgatttt cttgaggctt tcaaccaatt cgaacttatt cacgggtttc
    39301 atcaaataat tcacggcgcc taattgcata cctcctttca aataatcgaa ttcttggtat
    39361 cctgataaaa tcataaattc aaaattatga cgttcttttt gagccgcttc aataaattcc
    39421 agtccattta tttctggcat tgtcacatcc gttactacta aatcaatctg tcgctgttca
    39481 agtacagcta gtccttcttt ggcattctct gctgttgcta ctatttgaaa gcctaaactt
    39541 tgccaatcaa ttatcttttt taacccattc aaaatcatgt attcatcatc taccaataac
    39601 acttgataca cgctcatccc tccttgaatg taatagaaac ggtcagtcca cctgtctcat
    39661 tattctcaag cgacatataa taactgctgc cgaagctggc acgtaaacgt tcattcacgt
    39721 tttgcagacc gatagaaccg tgaagttcca ctttaggatg agacagtttt tcttcaatct
    39781 gttttagccg tttttcagtc atcccttttc cattatcttt gatgatgatc cttacccgtt
    39841 tgccttcctg caagactttg acacttagcg cattatcgaa tcgagtaaaa tcaataccgt
    39901 gtttaaaata attttcaact aatggctgga tcacaaattt aggtacttcg atcttttcca
    39961 aatcaggatc gatcataaaa tgataggcta ctcgattagg ataacgcatt tgatacaaat
    40021 agacatattt ttcgcaaaag tccagttctt cttttaaagt gatcgttttc tcttgattcg
    40081 tattgtttcg aagcaaagcg gaaaacgcat acacaacatc tgctaattct tcacttcctt
    40141 cgctgattgc atacattcgg atatattcta atgtattata taaaaagtgt ggattgatct
    40201 gtgcttgaag cgcccgcata tgtgcgtctt gctgtttgat ttctaatttg tagatgtctt
    40261 ctacaaactg attgatgcta tccaacattt cgttgatacc gattgaaagt tccttcagtt
    40321 catattccgt gtttgttatg tcgatccgcg tctctaaatt ccctgctgct gtttgatcca
    40381 tggcctgcat gacgatagat acttgctgtg aatatcgttt gaatgtttta taaagaaaga
    40441 taagcagtat attgtctaaa ataatgatac caatgatcag cggggcaaga ttttttcccg
    40501 tttcgatttt tacacttttt ttatctagta cagctaatat acgataatca tcagacagtt
    40561 cttggtgttg aacaaaattg tttttttcca gatcgtcaat cggcaattgt gaatttttct
    40621 tcagactctc tgtgatgatc atttcttgtc tttcaaacgt ttctttagct attttattat
    40681 catggaatgt gtatagtcga tttgtcgttc cagaaatcat gtataaagag agtccattga
    40741 atgacgttag attttctaaa atcatatcta gttctttctt ttgaaaacca ataaaaatac
    40801 ttcctgactc agctcctcct tgtgtgatag gcaacttgat ataaaaggcg tctagtaatt
    40861 ttggtgtccc cttttttatt tccccacctt tgttttctct agaagattca taatactccg
    40921 gtagttgatt caacacgata tatatcgcac ttagttcttc ataatttgcg tagagatctt
    40981 tgatttgatt tgggaaagaa actaattcat cggtactgtt ttgctgttcg tatacataca
    41041 tcagatactg atctatgggt tttgttaagt aggtcgtcac attttcgatc ttttcggaat
    41101 tccctacaag ctggtttttg atgatcctac tcaaggttgt tttatcattc agcattctgc
    41161 ttgtgctttg aacggcatca cttgtcatgt tttctgcctg tttttgattt tgttcccaag
    41221 tatggatact aaaaataacg gtagcagtca tcgttacacc gatcatgata accgcgtatt
    41281 tccgcaacag acgatttagt aaggttcctt ttgaaaatag aatccgaaac ttattcataa
    41341 ctttccaact gtttatctaa ttcttcacgc caatgtgttg tcatcgtacc attccaaacc
    41401 gtcaaaagac caatgacacc aaacaggatc aatcctttga attgccaagt cacgcctaaa
    41461 attataccgc taccaataat tatttttaga aaagacgaaa agctggaaaa aaccgaaata
    41521 aaacttagct ttagcagatt aggaacagag atcgtatatt cactatctaa aatcatactg
    41581 tattgataag tagcaaatag gcagagcaag gcaaaaagta aaataaaatc aatagctaaa
    41641 aaaatgagcc ctttgatttg aacagataaa tataaattgt aactcaataa agcagtacca
    41701 gataaaaata aactaaatag cagattgcct cttaaaaaat ttcttttata tatattccag
    41761 cctcttttta ttgtgatttc tttatactcg aatccgtaca aataaaatag ttcattgact
    41821 gttttccatg ccgcacctaa tccaaagatg atcccgccgg caaatgtata tatccagaaa
    41881 tacaaggtta gttttataat gacccagatc cggataaata actcttccaa aaccttccct
    41941 aacatatctt ctcctccaaa cattaagttt atactttctc attatacaaa aaaaggttgg
    42001 gcaatcagca ccttgacctg attattagag aaacaaacgg aaaaatagtt cctcatattt
    42061 tgtcgttttg tgagcgaata ccgagggtta gctgattgaa caccgtttac caagagtttg
    42121 tgaaatagcc gcttagtttc gagtagtaag aaaaattttg tgaatatggc tttccacatt
    42181 tttacatata tttagcttaa taccgagaaa ctggcttttg aacaccgttt attcggaaca
    42241 agaggttgtg acacgtcttt tgtcacaacc cctttgtctt tattcattat tattcttgtc
    42301 ggaactcatc atattgtttt tgcatttctt tcaatacctt atcatagcca gccttgtcca
    42361 aagcgtcttt caatttcggt aatgtttcat ctggatcaac agtccctgta ttcaatccat
    42421 ctaaatattg attcatgaca ttagaaatat tacttaattc tgttttgaca ctgtcagtgt
    42481 tgaagctgaa tcctaaaatc ggtgaagttt ctgcatcagc aatcgattga tcacgtttgg
    42541 caatcatgtc atccgtaata gactcttgtg tatatagaat cttgttgttt cctgtattcc
    42601 aagcagacat atgtgtattt ggctgataac catctagcaa tttgatttta tcatctgaac
    42661 catcgacttt ttcccatgct tctccctcga tcccccaaac aagcccattc aataattctg
    42721 gatcactgtt aagcagccct agaagttcta ctgatttttc tttatttttt gaagtatttg
    42781 atacgacaaa atttgccatt tgtgcttgag ctgtagattt tagtggaaca gtaattgctt
    42841 ttgagacaag ttcttgtttt gctgcatttg tcaaaattgt atcgccataa tcatacggtc
    42901 cttgtgtttc tccacgcatt agccaagtat ttccttctag tggataatcc ttattgctcg
    42961 ttgcagcgtc acttggaatg agtccttgtt tgtaccagct atgcattgta cgtaacagtc
    43021 cgatatacgt ttcattatcg tattggttga tgattttcga atcatctcca tccagatcaa
    43081 tagcaaatgg taacgtattc cctaaaggat aatcaaagtt cccttggact ttgaagcctt
    43141 gtccaatggc aaaagctgcc gtgtttggtt ctttttcatg gaattccttc aagacttttt
    43201 ctgcatcagc gtaagattgg attccatcaa ttgaaagatc gtatttatct agtaaagctt
    43261 tgttaaaagt aagtacttgt tgagcaaaga catttccatt gactgggaaa gcatataatt
    43321 ttccattgac cagatttcct ttaatatatg cttcatctaa atcattataa gctttttcag
    43381 cgtacttagg cgctaattca gtcaaatcag caaaggctcc tttttgggca ttggaaacat
    43441 agttgttcgc aaaggcgata tcataatttt ccccggaaga aatgatgacg ttcattttct
    43501 tttcgtaatc gccccagcca atgtaattga tattgacttt tacaccgatc tctttttcga
    43561 ttcgtttgtt tgctacttcc atcaattgat cgaaattatc tggtttatct ccgatttgat
    43621 acatctgcaa agtgtccgaa ttgctagctt ctgcctggtc tttagaacca gaacctccgc
    43681 atgctgctaa tgttcccaac aacaacgctg aaacacctgt aaaggctagt ttcttccata
    43741 acttcatttt tcttcctcct aattttatga tttatatttt tgtttcttta ttcttttact
    43801 ccgccgattg ttagaccttt gacaaaatgc ttctggaaaa atggatagct gatcgcaata
    43861 ggcaacgtcg agatgactac gatcgccatt ctggcagatt ctcctggaac tgaggctaat
    43921 ccgcctgtca attggcttcc tgctcctgaa ttttgtgtca gatattgaat attactttgg
    43981 attttcatca aaagatactg tagtggaact agcttatcat cttggatgta tagtaaagca
    44041 ttgaaccagt cattccagta acccaatgcg gcaaataaac tgattgtcgc aatacctgga
    44101 acagctaacg gtaaaacgat ctgaacaaat attcgcagtt cgctagctcc atcgatccgt
    44161 gccgattcaa taatcgaatc agagaccgaa cgtttaaaga atgtgcgcat cacaataata
    44221 ttgaagggac tgacagccat tggcaagatc aatgcccaaa tcgtatcttt taactgaaga
    44281 agatttgtca taacaagata attggccacc atccctggtg agaacaacat cgtaatcaaa
    44341 caaaaaacgg taaaaaaacg acggaatgga aagtttgacc gtgaaatcgc ataagcatat
    44401 aaagaagtag ccgtgctatt gatcagtgta cctaaaacag taacaagtac agtgacgaaa
    44461 agcgcttgga aaattttatc ctgcatctgg tcgaacaagt acgtataacc aaatatactg
    44521 aacttttctg gccaaaaact gtacccgtga gtcgacaata tggactcatc ggtaaaagag
    44581 atggcgataa caaatataaa tggcaaaata caggaaatag caaagagtgc aatcattata
    44641 ttagagaaaa aattagccgt cttattgaaa gagcggactt ggactctctc cactttcttt
    44701 tttttcattt ttttcctcct tagaataatg cagaatcgct atcaaaacgt cgggcgatcg
    44761 tatttgtgat catcaacaag acaaagccga caactgattg gtaaagtcct gcagcagccg
    44821 tcataccaaa atctcctgtg gatgtcaggc cattgtagat atacgtatcc aaaacttgag
    44881 tcacatcata taatgcaccg gaattcctag gaatgttata aaacaggcca aaatcagctc
    44941 ggaaaatgtt tcctacggct aaaatcgtta aaatcgtcat tagtggaata agttgtggta
    45001 tcgtcacatg tttgatctgc tgccatttgc tagctccatc aaccatagca gcttcataat
    45061 aagtcggatc gattcccata acagatgcaa agtaaataat gctgttataa ccaatccctt
    45121 tccatgtacc aatgaacacc aagataaacg gccaatattt cggttcatta taccaattga
    45181 cagcttctcc tccatgtcca gtgatccact gattgaatat tcctttgtca ggacttaaaa
    45241 aagcgtaaac aaagtaactg ataatgaccc aagatagaaa ataaggtagt aaggacatcg
    45301 tttgataaac tttaacgagt cgtttatttc tcaactcgct cataatgatt gcaaatacga
    45361 ctgagatcag cagattcaaa aggataaatg ctacattata taaaattgta tttttagtga
    45421 tgagaaatgc ttgatccgaa gaaaataaa
//
LOCUS       DAZUMM010000013        45092 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000013 DAZUMM010000000
VERSION     DAZUMM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.013"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..689)
                     /gene="msrA"
                     /locus_tag="ACG8E6_000657"
     CDS             complement(162..689)
                     /gene="msrA"
                     /locus_tag="ACG8E6_000657"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5061102.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(773..1450)
                     /gene="pgmB"
                     /locus_tag="ACG8E6_000658"
     CDS             complement(773..1450)
                     /gene="pgmB"
                     /locus_tag="ACG8E6_000658"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS5061103.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(1447..2577)
                     /locus_tag="ACG8E6_000659"
     CDS             complement(1447..2577)
                     /locus_tag="ACG8E6_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS5061104.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(2577..4760)
                     /locus_tag="ACG8E6_000660"
     CDS             complement(2577..4760)
                     /locus_tag="ACG8E6_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFS5061105.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(4757..5782)
                     /locus_tag="ACG8E6_000661"
     CDS             complement(4757..5782)
                     /locus_tag="ACG8E6_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5061106.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(5827..6654)
                     /locus_tag="ACG8E6_000662"
     CDS             complement(5827..6654)
                     /locus_tag="ACG8E6_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS5061107.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(6651..7718)
                     /locus_tag="ACG8E6_000663"
     CDS             complement(6651..7718)
                     /locus_tag="ACG8E6_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFS5061108.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(7744..8580)
                     /locus_tag="ACG8E6_000664"
     CDS             complement(7744..8580)
                     /locus_tag="ACG8E6_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5061109.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(8577..9446)
                     /locus_tag="ACG8E6_000665"
     CDS             complement(8577..9446)
                     /locus_tag="ACG8E6_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5061110.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(9516..10799)
                     /locus_tag="ACG8E6_000666"
     CDS             complement(9516..10799)
                     /locus_tag="ACG8E6_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5061111.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(10813..12498)
                     /locus_tag="ACG8E6_000667"
     CDS             complement(10813..12498)
                     /locus_tag="ACG8E6_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFS5061112.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            12773..13798
                     /locus_tag="ACG8E6_000668"
     CDS             12773..13798
                     /locus_tag="ACG8E6_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5061113.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(14393..18046)
                     /gene="rpoC"
                     /locus_tag="ACG8E6_000669"
     CDS             complement(14393..18046)
                     /gene="rpoC"
                     /locus_tag="ACG8E6_000669"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFS5061114.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(18125..21742)
                     /gene="rpoB"
                     /locus_tag="ACG8E6_000670"
     CDS             complement(18125..21742)
                     /gene="rpoB"
                     /locus_tag="ACG8E6_000670"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS5061115.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            22470..23438
                     /locus_tag="ACG8E6_000671"
     CDS             22470..23438
                     /locus_tag="ACG8E6_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5061116.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            23456..24361
                     /locus_tag="ACG8E6_000672"
     CDS             23456..24361
                     /locus_tag="ACG8E6_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5061117.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            24358..25164
                     /locus_tag="ACG8E6_000673"
     CDS             24358..25164
                     /locus_tag="ACG8E6_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5061118.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            25321..26289
                     /locus_tag="ACG8E6_000674"
     CDS             25321..26289
                     /locus_tag="ACG8E6_000674"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFS5061119.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(26606..27130)
                     /locus_tag="ACG8E6_000675"
     CDS             complement(26606..27130)
                     /locus_tag="ACG8E6_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFS5061120.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            27417..29228
                     /gene="scm"
                     /locus_tag="ACG8E6_000676"
     CDS             27417..29228
                     /gene="scm"
                     /locus_tag="ACG8E6_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFS5061121.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(29383..30369)
                     /locus_tag="ACG8E6_000677"
     CDS             complement(29383..30369)
                     /locus_tag="ACG8E6_000677"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS5061122.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(30392..30958)
                     /locus_tag="ACG8E6_000678"
     CDS             complement(30392..30958)
                     /locus_tag="ACG8E6_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFS5061123.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(30972..31388)
                     /locus_tag="ACG8E6_000679"
     CDS             complement(30972..31388)
                     /locus_tag="ACG8E6_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFS5061124.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(31845..33779)
                     /locus_tag="ACG8E6_000680"
     CDS             complement(31845..33779)
                     /locus_tag="ACG8E6_000680"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5061125.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            34081..35016
                     /gene="pplA"
                     /locus_tag="ACG8E6_000681"
     CDS             34081..35016
                     /gene="pplA"
                     /locus_tag="ACG8E6_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFS5061126.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            35106..36209
                     /locus_tag="ACG8E6_000682"
     CDS             35106..36209
                     /locus_tag="ACG8E6_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFS5061127.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            36222..37175
                     /gene="menA"
                     /locus_tag="ACG8E6_000683"
     CDS             36222..37175
                     /gene="menA"
                     /locus_tag="ACG8E6_000683"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFS5061128.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(38110..39456)
                     /gene="gor"
                     /locus_tag="ACG8E6_000684"
     CDS             complement(38110..39456)
                     /gene="gor"
                     /locus_tag="ACG8E6_000684"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFS5061129.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(39590..40843)
                     /locus_tag="ACG8E6_000685"
     CDS             complement(39590..40843)
                     /locus_tag="ACG8E6_000685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFS5061130.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(40849..41772)
                     /locus_tag="ACG8E6_000686"
     CDS             complement(40849..41772)
                     /locus_tag="ACG8E6_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFS5061131.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(41993..44485)
                     /locus_tag="ACG8E6_000687"
     CDS             complement(41993..44485)
                     /locus_tag="ACG8E6_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFS5061132.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(44492..44959)
                     /locus_tag="ACG8E6_000688"
     CDS             complement(44492..44959)
                     /locus_tag="ACG8E6_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFS5061133.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT        12983 a         9645 c         8290 g        14174 t
ORIGIN      
        1 tatagtgttc tcttaccaaa tccttgtgac ttggagctag gcgacaggtt cacaacctct
       61 ccactaaatg gtgttcaatc agctgaccct cagtattagc tcaaaatgct ggttttacaa
      121 cctctgatat acggtgttcc ataagtagct ttttcggctt cttatttctt gacgccccaa
      181 tgtcgacgaa ggaaacctgc acgtccagaa ccttctcgat aaagattgta atggagcttg
      241 tttttcttgt aataatcctg atggtaatct tctgcaggat aaaattcgct tcttggttcg
      301 atttttgtca cgattggctt atcgaatcgc ccgctgtctt gcaatgcttg ttttgaacgc
      361 tctgcaatat ttcgttcttc ttcatcatta tagtagatca ctggacgata agaatcaccg
      421 cgatcagcga actgtcctaa tgcgtcagtc ggatcggttt gctgccaata gatttccacc
      481 aactgatcat aagaaataac ttctggatca tattcgatct ctactgcttc tgtatgccct
      541 gttgtgcctg ttgttacttg ttcataagtt ggatttggta catgcccgcc agtataaccg
      601 gatgtcacag aaatgatccc tggctgctct tcaaatggtt tgaccataca ccaaaagcat
      661 ccgcctgcga aaactgcttt ttttgtcata taaaatcccc cttttttctc atcatacctt
      721 gatttccaaa aggaggataa cgttttgctt gtgacagaaa aaacggaaac tattatatac
      781 gtgatacggc ttctgataaa tgccaaaacg aacgtacttg atcaaaaacg gttaaatcag
      841 gaatcacata ttcagcagaa ggaagattta tgttttcacc tattcccaat acatgcatgt
      901 taccagccaa agctgcttga caaccagctt ctgaatcttc aatgaccaag catgcatttg
      961 gcggtacatt caattgctgt gctcctttga gaaaaacttc tggatcaggt tttgcttttg
     1021 atacttgcgt gccgtcaata aggacatcaa aataacttgt caaattcgtt ttttctaaaa
     1081 tgaggcgtgc atttttgctt gctgatccca atccgatctt gatgtttgtt tttttcaagt
     1141 actcaagaac agctattgca ccaggcaaaa cggatgtttc atccattttt tgaatcgctt
     1201 gaagatatag attgtttttt tgctcagcaa aggctgattt ctctttttct gagaatttct
     1261 gttccatttt tccaaatgac agcaatcgct ctagtgactc ggttctggat attcctttca
     1321 gttgctcgtt ttctttctca gtgaatggaa tggacagctg gttagctaaa accagccacg
     1381 cttcgtaatg ataacgagct gtgtctacaa gaacgccatc taaatcgaat agtgctgctt
     1441 tgattttcat cgattacctc cttgaaaatg aacgacttga agtaaagatg cgatgttttt
     1501 agctgtgcga tgaagattat cagtggcttg ctgtaaagct tcttgtaaag gcacagctgc
     1561 aggaaggatc ggaaaaacgg ccgaaatgcc aacatctgct gctttttcgc ttcctttacc
     1621 aacacttccg caaatagcta tcacaggaac gccctctggt actcttctag caatcccaac
     1681 tggcgctttt ccttttagac tttgtccatc tagacgtcct tcgcctacga ttacgaggtc
     1741 tgcttgctgt acccgtttat cgaactccag taaatccaat acgtagtcga tgccagagac
     1801 tattttccct gaagcaaatg ctaacagtcc tgcacccatt ccaccaccag cactactgcc
     1861 cgatgcctcg attacttgag gggcaaatct catataaaaa tcatgcatgg cttggtcagc
     1921 cactgctaat tcattttctt tcaagccttt ttgtggtccg aaaacaaaag aagcgccttc
     1981 ttttccgcat aaaagattag taacatcagt aatgacagtg attttggctt taatcagctc
     2041 aataggcacg ttcgttttat caactgtagc aattttacct agattttctc ccagtgcttc
     2101 tactttttga ttatcttgat cgtaaaaagc ataacctaga ccttctgcca tgccgatgcc
     2161 gccatcgttg gtactacttc cgccaacgcc gatgatgatg tgtcgaacac cttgttgcaa
     2221 caattgtcgt atcagttctc ctactcctcg cgtggataac ctaagtggct gttgttggtt
     2281 tagtgggaca tctttcaatc cgacgatttc tgccatttca aaaatcgcag tttgaccgtc
     2341 tgaagcgtac tggatttctt tgtttttgcc aaatgcatta gtaacgactg ctgtttgccg
     2401 cttcaacctt aaactgtctg ctaatgatga aaccgttcct tctcctccat cgcctatagg
     2461 tagcagatca tattcagcct gaacaaaaac ttcttgaaaa cctgattgaa tggcttgtgc
     2521 tgcctctaag gctgtcagac tttctttgaa agaatctggc gcaatcagaa tacgcattat
     2581 tttcccctcc gttccaagat cagttctttt tccagctggt attggtctcc attgacaaaa
     2641 ataggtaatg tttctttcgt ctcttttttt attactattt gtttttttgt caaaacaaat
     2701 agcaacctgt gatttttcca aataaaagga aaagaaattt tttcccatgc tgaaggaagt
     2761 cttggagaaa tagaaagagt tccattcgtt actgtaatac ctgaaaaacc aaatataact
     2821 gctagccata gtgctcctaa tgaagcagca tgaattccgt catctgatga atgcggatct
     2881 ggtcctaaat caatcaggca cgcttcttgg aataaatgat acgcccattc catttcttgg
     2941 atacggcaag cctctatggc gtgaatcgct ttactcaacg atgagtcgtg gattgttttg
     3001 ttttcatagt aatgcaaatt cttagaaacg attggttgag aaaaatactc aggtaataag
     3061 tataacagca tgacaagatc tgcttgcttg ctgacttgga gttcatttac ttcgctacgg
     3121 gcataatcaa gtaaaataga ctggctgcca ggacgttctt tgtaagtggt caaatcaata
     3181 actggcttac tgagatacgt gtcatcttgc ggaatcagct gttctttgtt aggcactggc
     3241 aaatataact tatctaaaaa attttgacat tctttcaaaa aaagcggatc gccagcttga
     3301 tatttaacag ctaaggccac attataatgc gccagatagt tcgtatatgc attattgtcg
     3361 atatattctg tgtattcatc tggaccaatg actctgtgta tctctaaatg atcatcgact
     3421 tttacagctc tgctcagcca aaaagctgct gtttcatgaa tgagcgtttt tccctttgtt
     3481 tctaaaaacg attgatcctc tgttgcttgg acatactctt gaacggcata agcaaggtct
     3541 gcactgatat gatgttctgc ggcagctgaa gcaacttctt gtcttgttcc ggttttgata
     3601 ttcaatgcag caaatttggg tgtctcttcc tgaccagtgt gtgcgctttc ccatgggaaa
     3661 agtgcacctt gatagtgata atctcttgct ttttgccgtg cgccttctaa tcgatcataa
     3721 cggtacagca gcaaattctt tgctacttca ggaaactgat ataaaaagaa aggcaggata
     3781 aaaatttccg tatcccagaa aacatgccct ttataacctt cgccagttag tcccttcgcc
     3841 cctacactaa cacttggatc attgcgggga gtcataatct gtaaatggta agcagcgaag
     3901 tccatcgcta actgatcgaa agaagaatcg ccttggatcg ttatccgatg ctgatgccaa
     3961 aaatctgtcc aataagacat cgactgctct aaaatttctg tataactaac gcttttctgg
     4021 gcttgggcaa tacttttctt aaacgggtct gtttgctgaa gagaggtaga gagcacgatg
     4081 atcttctcca gcgtaaagga cgtatttttt ctgatctttt ctgaaaagga agcggtgatc
     4141 cggcgatttt ttgcttggaa tacacctgct ttattgaatt tagctgcaat cccaatatca
     4201 attttacttt ctgttgtctg gtaggatgca ctgatcaaat cgtcaccaaa tactcgcagt
     4261 tctttttcaa ccaactgctg tgtaccaaaa ttggtttgct gtccatcgat acccgttttc
     4321 acatctactt ggcagtcttc ggtcaacggc ttgatgacca ctttcagggc tgcctgatgg
     4381 agattatttt gatcagcaac acgacaagta atgacttcca ctttttttcc ttgagcagtt
     4441 tcaatgagcg catagcgttt aagctctccc atttctaaat ctagtgcccg ctcgtatgca
     4501 agaactttca tcgtctcaag agaaaaatgt tcaccgtcga tttttatttc ccatctgatg
     4561 acatctggga gattcactaa ctcggctgat tctgctccta aaggtcggtt atatacacca
     4621 gctgcaaaca ttccccttac ttgattccga tagtcttcct cctggcttcc tctgatacca
     4681 aaaaatccat ttccaatggt catctgactc gcatacttat tgatatactc taaatcaaac
     4741 tgtttttctt ctactttcat aaggtaataa cttctcctgt ttctgccgaa cgatataaat
     4801 tttccaccag attctggatg acatatcctt gccagccatc tgcaatcatc tgagtctggt
     4861 caccgagcgc ccgattcaca aaatgagcca tacttagttc atgtgggtca ttaactggcg
     4921 tggtcttttg taaggaaacc agctttccat ctacatcttc gaatagttcg cctggataca
     4981 gattagcacc tgcctgatcg ccacaaaaag ccacattcag tttgtcttct ctgatattca
     5041 aagcaaacga agtattcagt tggatgatct tgccgttttc taattccagt gtgccaaaaa
     5101 ttgcatcttc tactgtgaat ttgcttggat cccatttccc gaatttccct gaattttttt
     5161 tcgtaccaat tttttggaag ctgtacgccc gaacgctttt tactttagga aatccagtaa
     5221 tatacatcgc tgtatcaagc atgtgaatgc cataatcgat caaaggcccg cctccttgaa
     5281 gttctttgtc gataaacgtt ccccagcctg gaacaccaga ccgacgaagc gcattcgctt
     5341 ctacgtaata aatatccccg agaacatcaa gatgattctt gatcattgtt gcttcgtcag
     5401 aaaaacgatg ctggaatcca taagccaaaa tctgcccgtt tttatcggct gtctctgcca
     5461 tctttcttgc ttgatcagct gtcatagctg gtggtttctc gcacatcact gaagcattag
     5521 cttcgagggc agccaataca tgttcgaagt gaaagcgatt gggtgtacag acactcacga
     5581 tatcgggaga aatctcattc agcatttgtg taactgatgt atacgcatga gaaaatcctg
     5641 tacgtcggca aaagtcttct gctcgctggc tgactggatc aaccacagcg gccaattcta
     5701 agacatctga tcttttttga taataagcag catgtacttt ttctgctact tgtccagcgc
     5761 ctatgattgc tactttcttc atttatttct ctctcctttg cttatttact tattgttatt
     5821 ctttctctat tctttctcta aatcatttaa atgacgcgct gtttccagac agtttttcaa
     5881 gtattgcaat gattcctgat aaattttcac ttcattttct cctcgcaaac gacattcata
     5941 taccatataa ccttcatact cattttgaag tagcgtttga aaatggcgag taaaatcaat
     6001 tgcgccagat cctggttggt agcgatgatt atctgctaaa tggatatgac ccacatactc
     6061 tttttgcgta gtcaatgctt ttgaaatatc atcttcttca atattcatat gataaaaatc
     6121 agcgatgatc tgcacatgtt tcaattgatt agagtcaatg taatgacgcg catccgctaa
     6181 caaattgatc atatggtctt ggtatcggtt caaaggctcc aaataaataa ccgtacctgt
     6241 ttcttcggct actttatcta aataacgaag tgattcgctg actgctttat agtctccttc
     6301 tttgcttcgg ggtgaaacca tcggcggtaa tcgataagtg aacattcccc aagctgctgg
     6361 gataacgatt cctttgcctc caacctcgct tagtgcacgc aaaatctcag caatttgacg
     6421 tacaccatta agacgccgtt cttcgataaa gtcaccgatc cacccatcgt agccgttaca
     6481 agctgtcact actgggatcc ctgtttcttt gattgcctgt ttgactgtgt cgatattttc
     6541 tattaacaac ttaccgtcga tttcgtattc ttcaaatcca cagtctttga taaatctaaa
     6601 tttttctaaa atgtctcttg ggaaaaatgc ttgatcttgc gtaccgaatt tcattgttct
     6661 cctcctcgga cttgaacgcc cattttgata ctttcttctg ggtgctggtc tacataacgc
     6721 atgtaagctt ctgcgctttc tgcaaatggc acaactggat caatgatttc ttcacaatcc
     6781 aagtaaccgt tcatcaataa ttcccaacat gtgtcctcga ttcgtttacg gttccatctt
     6841 gggtaatctg ggtttggttc actagatgct cgagcaaaga cgatttttgc attattgaaa
     6901 tgtgcttcgc gaccaagatt aaacccagct ggaaaaggtt tagcaaaggc aacataagaa
     6961 atgatcccgc catagccgat ccctattaaa gaagcttgta atgcggcagc actgccactc
     7021 gtttcaataa ctgaatctac tccacgttta tctgtcaatt cctttagacg aaaaccgaca
     7081 tctgctttca taggatctag cgtgtaatcg gcaccgtgtt tttctgctaa ttctctacgc
     7141 tgcgctatgg gatcgatccc aacgatggta gtagcacctg cttttttagc taattgaatc
     7201 gcaatttgcc cgatagcgcc taaaccgaag attgctacag catcacctgc tcgaacatgt
     7261 gcttcccgga taccacctaa agcgaattga gcaggatcgt aacatactgc atttttccat
     7321 tgatccaacc gatcaagttt acgtagttta taattatcaa caccgtttgt aatcagcgtt
     7381 tcacgaatgg gtccataagt gcagactaac tctccttttt gatactctgt caccaaactg
     7441 cccgtttctt cgatcgtacc gacaatcata tttcctaatt gaaattcgcc aaataccaca
     7501 ccgcgttggc ttccttcttc tcttggcaag aataggcgcc attcttctga aaattcttca
     7561 tcgataaatg gacttaatcc tctaaagtcg acaatttctg ttcctggttt tggagtagcg
     7621 aaaattactc ggatacgtac ttcgttcttt tcgatctcac ggtcttcgta atctttgaat
     7681 ccggctattc ttggttctaa tgcaactaat tttttcatct tttctttcct ctttcttttc
     7741 tttttatcct tttacgccgc cttcgactaa atcaccttta ataaatcgtt cagataacgc
     7801 atacataatg atgactggta aagcggtcac caatgaagcg gccatcattc gtccccaaat
     7861 gtagtctggt gtactgaata atgtgttcaa accgattggt aaagtaaatt tttcggaggt
     7921 cgacaggaaa atcgaagcaa ataaataatc attccacgaa accataaagc aataaacgaa
     7981 gacagaaatg attccagaaa tagccaatgg tataatgatc cgaataatga tctggatccg
     8041 gctaagacca tcgatcattg ctgcttcttc aatatcagtc ggaatcgtgt caaaataact
     8101 ttttagcatg aatatcgcag taggaagtgt ttgaacgacc atcgtgataa tcagtgctgt
     8161 attggtatca tacaaaccta gaccggaaat gattttaaat aatggaacaa cgatcaaaat
     8221 accggaaaac atataaacgg tgtaaaagct agcattgatt ttcgagcgag ctttgaagcg
     8281 tagttttgac aaagcgtatg ccccgctgat cccaatcact actgcagtta cggaggctat
     8341 caaggagatc actaaactat ttttgaaata catgatgaat ggaaaaatct cagcattgaa
     8401 aatatcctga taatgttcaa aagtccattc tttcggaaaa atagtttgtg tcgttgcaat
     8461 tgcttcttgt gaatccttga atgatgtcat cagcatgatc aaaaatggaa acaaattgac
     8521 agctaatagc aacaaaatcg tcgcgtaaaa taagattttc ctgctctttt tatttttcat
     8581 ttcaagtcca ccttctttcg tgagagcaag acgaccgctg caataatgat gaacaataga
     8641 atcgatatag ctgctgcttt tccaagatca ttgaacgcaa aggctgtctg atacaaataa
     8701 acgcctaaga tattgacttt attcgtcagt agatagacat ctgtaaacat atagaacatc
     8761 cagatagatc gtaaaacaat cacagttgct agtactggtt tgattgccgg cagtgtcacg
     8821 atcttgaact tctgccaagc atttgcaccg tccatttcag ctgcttcata aagcgttttg
     8881 tcgactgttt gcaaaatagc caaaaatgaa ataaacgcat acggatagta tctccagata
     8941 gcaaacaaaa cgaccataaa gaagctagca actggctggt cgaaccacag cggtgcttcg
     9001 ttgaaaagct gcaacctatc tactaacaga tcattgacga ttccataact attattgaac
     9061 atatatttcc aagcaaaaat caaagaaata gatggcgtaa cataacttaa taaaatcagt
     9121 gaacgcaccg ttttcctaaa tctgaacttg cggttaagca aaatagctac ggctaatcct
     9181 aaagctgtac tgcctgctac aactaagaat gtgtaagcaa ctgttaaacc taaagaacga
     9241 taaaattcac tatcacttaa aatttcaaga taattgctta atccgataaa aacggcgtct
     9301 gagttaggat tcagcggcat atcgaaaaaa ctgatctgaa tattggaaag cattggaatc
     9361 agcactaaaa cagctaataa aagcatgctt ggtgctaaaa gtcccatggc taattttaag
     9421 tcagaatgtt tccttggacg attcattgaa acctccccct gataatctgc caatagctat
     9481 ctactttttt cgaacaccat tggcagaact tcttgttatt tcgtattttt ttgtgcgtca
     9541 gctaaagctt gatcgacatc ttcgtttccg acagttactt ggttcactgc ttttccgatc
     9601 acacctgagc ttgtgatatc tcccattttc gtgaaattct tttccccgac tagtccaaag
     9661 acttgtactt catcaaaagc actagcaatc tcagatggca gctcgccaaa agacttgatg
     9721 acttcatttt ctttgtaacc atccagttcg actacttgtt tattcactgg ttgagcacca
     9781 ccaggagaca tcaagaccca cttttccatg tttttcggtt gagataagaa agctgtgaat
     9841 gcttcagcag cctctttttg tttctcgtct agaccagaag aaatcgtcaa tccagatact
     9901 gtgccataga cagcttgatc tttttgtgtt gggatagcaa aaccgatatt ttctgagttc
     9961 cccgcttcat aaacagaagg caaaatgtaa gttgaataga ttgccattgg tgctgttcca
    10021 ttcatgaaag catctttgat ttctgtcaca tcattagagc ctggcatcgt gtattgggat
    10081 aagttttgat aaaacgacaa tgcttctctc atcttttccg tatcgatcgt tacctcgcct
    10141 tcatcatcca aaacatttgc tccatttgac aaagcaaatt gagagaacgc ttgctcactc
    10201 attgttcctt cagcagtagg catagcaatt ccatattgtt tattctcttt atctgtgaaa
    10261 tgttgagcaa tttttaaaat atcatcccaa ttttctggtt ctgaaaaacc agcttctgat
    10321 aatttttctt tgtcatacca aataccttga acccagccgc taattggtgc agcaatatag
    10381 ctttttccat cttctgaacg aacaaggttt ttggctccgt cataataatt atcttctcca
    10441 gcttcctcga taacgttttg gactgccgtc tgatcaatca gttcatcttt atccatcact
    10501 ttagcaaaat cctgactgac ttccattact tctggtaatt taccggaacg agctaacgta
    10561 acgatttttg tattgaacgc tgattcttct accggcactt gcttgatttt gatgtctgga
    10621 ttttcttttt caaaatcagc aaccagttca ttgatcacac ttaatcgttc ctgttcgaca
    10681 gaagagtgca tgaattgaat cgttgtctgt tgtgattcgt tagagctttg actttcacca
    10741 caagcagtaa gaagaaatcc agtagctagc agacaggtag taacaataat ttttttcatt
    10801 tttttccacc tcttatttta ttattttcag ccacatatat tcatacggct ggaatatcaa
    10861 ctttcgttct tgactgcaat ggataatttg tccagaaaac aggtctaggt aatctccttt
    10921 tggtaaaaga taacctgctg attgattgga aaaattatgg ataactaaga ctttctcttc
    10981 cttcgcttcg cgaataaaac tgataaaaga atctgctgag tccagtaagt aaatcaaact
    11041 attcggatgg aaggcttttt ctttttttcg aatagatagc atctgggtca attcaccata
    11101 tactgctgtt cttaatggat ctgtctgcaa ctctttctca atatctgcta acaaaaattt
    11161 ttgccgattg atttttcgat tctctcctgt tttttctacg cctttgtaat catttcgact
    11221 tcccagcaca ctttgaatat aaattgccgg cacgcctggt aacgttacca aaaatgaatg
    11281 agcagctaaa aagcgtttgc ttctccattc cgtcgtatct gttggagaac ttaacgcatc
    11341 tagatacgta gcgtttatct catatggact ggtcgttcca tctgggtttt tcttgtagtt
    11401 tactcgggca ccctctttct tcagctgttc cactaattca aggatcgttt tttccggcag
    11461 aatacctcgt accggattca atccgatacc gtcatgggag gctaaaaaat taaaaaaagt
    11521 tgtttttttc gatgcgaaat cgatggtttt taaccattct ttcaacttac ttgtattacc
    11581 cgaacgaatc gcttgaaggg tcaaaggtgg tagcgaaaac tgatagacca tctgtgcttc
    11641 attctcgcca ttcccaaaat aactgatatt atctttgtgt ggcacatttg tctcagtgat
    11701 caataccgtt cctggagctg cttcatcagc gattgcacgg aataattgga tgattttatg
    11761 cgtttctggc aggtgtatac aagaagttcc tagttctttc cataaaaatc ccacagcgtc
    11821 caagcgaata aatgaagcgc ctttttctaa gtaaaacaaa agaatatcga tcattttcaa
    11881 taaaacttct ggatttttaa aattcaagtc tacttgatct tcgctaaaag tcgtccatac
    11941 gtgtttttct ttcccgtttt tgtcaatgaa cgttgtaagt aacgggctgg tccttggtct
    12001 agtcacgctg cttgtatctg tatccagtgg caaatcaata aaaaaatctt tgtacgtatc
    12061 attcaagttt aaatattcct gaaaccaagt actttcggca gacatatgat tgcagacaaa
    12121 gtcgaacatt aaccgtgttt cgcttctcat ttcttctata tcttcccatg tgcccaaacg
    12181 gtcgttgatt gcctgataat ttacaacgga gaaaccatca tcagatgtat atgggaaaaa
    12241 tggcaagaaa tgtacaactc taaatgtatc tgccaaatac ttttgataaa atttcttaaa
    12301 tgtacgcaaa gttggttgct gctcttcaaa aaattgatcc ccataagtaa tcaagaaaat
    12361 atcttcttcg tcccaatgca gtttgcctgc ctggcctttc ttctgctgat aaacactgac
    12421 tttttgttca atcatagagg cggttttctc agacaaatct gagtcgcgat aaatcgcttg
    12481 taaaagtttt gtatacatcg gattcccctt tcttttttgg gagcgttccc aacgatttga
    12541 tcatattatg gaaaacgctt actgtcaata gcattttttt aaataatttt gatatacaca
    12601 aaaatacaaa taagaaagct tatgtatcaa taattaatag cttcttaaaa aaatatggtc
    12661 tctcatgatc atgcaagcta ttcctaaatt ttcaaaatca tagacaaaaa agaatgtcta
    12721 ttgtactatg gaattattct aaaacgctcc caaaaaggag gtgcatataa aattgaaacc
    12781 tactatttat gacattgcga aaaaagctaa tgtctcaaaa tcaactgtct ccagagtatt
    12841 aaataaccag ccaaatatct ctgataaagc aaaagcaagt gtacttcaag caatcaaaga
    12901 attgaattat gaaccgagca aaattgcaag aggactgtca ggaaatggtt ttgacgcgat
    12961 tctagcatta tctcatcgta cttctcatac gacctatggc aatccttttt tttcagatgt
    13021 tattcaatcc atttctgccg tttctgaaca aaataacttc gacttgattc tacaaacggc
    13081 aaaaacacca gaagaagagc tagaaaaatg cgcgaaaaag atccaagaaa aaatgatccg
    13141 aggaattatt attttaagct ctacgatgga tgaaacactt ctttttgaat tggatcaata
    13201 caatattcct attgtggtaa tcggaaaagt cggaaacgac tacacgaaca tctactcagt
    13261 ggacacggat aatttccagg atagttacga tttggtggaa acactgatca agaatggaca
    13321 ccgtcatatc ggctgtctcc atgctccgac agaagtttat gtatccaatg atcgtctcaa
    13381 tggttaccgt caatgcttat ttgatcacca cctagatatc cgtaatgaat ggattataga
    13441 cggcggcttt cgcttagaag atagcatggc agcaatgaat gatttaatga agctacgaga
    13501 aagaccaact gcgatttttt ctaccgatgc attaaaatca ttgagtattt acaaagtggc
    13561 agaacaatct ggaaagcaga tacctgatga tctttcgatc gcaggattca gtgatccctt
    13621 attgtcctcc ctattttcgc cacagttgac tcggatagac attccgacaa atcagttagg
    13681 aatcaaagcc acagaacttt tattccaact aatccaccac caataccctg aagaaaaaag
    13741 aatcattctt tcaaccgaga ttactctcac agattcgatc caacaacaag aaatgtagaa
    13801 cagaattata aaagaacaga aacactgagg ataagtcagt tactctgttc ttttttcaat
    13861 cactcatccc tcgttacccc ctcccggtat tttagcgaat ttttcttatt ttccatctgt
    13921 tttagatagc tgtggataac tataatgttt gtgttaaact attttttcca cagggaagaa
    13981 aaaactattt ttaaccacgt tgtgtataag tttttatagt tcgttttttt aaacacattt
    14041 acccttattt caatccatgt tatttgggga tacttatctc taaaacataa atagaataac
    14101 agacatgtct actggttttg ttttattcga ggaattattc aataatagct agcaaaatat
    14161 taaaaataac cgctctatct tcccatcaat ttattttgca ttcaaaaaat gatctatctt
    14221 tacttttgtt gattgacttc tcttggaaga tgatcgttcc gataccttaa ttggatacga
    14281 accgatgttg ttaagatcct ttcccacttt tcttctagtt agacaaatta ttttagacaa
    14341 accaaaaaag aggttgtgac aataatttgt cacaacctct tttttaaaat atctattttg
    14401 tttgattgtt cattgcgtct tctgcagcca tttgcgcttc aatatctgaa atactgtaga
    14461 cattttcgct tgctacaccg acttcttttg gttccatgtc acgataacgt tgcataccag
    14521 taccagctgg gatgatctta cctataataa cattttcttt caaaccaaga agaggatctt
    14581 tcttaccgcg gatcgcagca tccgtcaaga cacgagttgt ttcttggaat gatgcagcag
    14641 ataagaaact atttgtttct aatgatgctt ttgtgatacc tagtaagaca ggtcgagaag
    14701 ttgcaggaac gcctcctgag actagcgtat tgtagttttg atctttaaag tctgcgatgt
    14761 ctaataatgt acctggtaag atatctgtat cacctggatc cattacacga actttacgca
    14821 gcatttgacg aaccattact tcgatatgct tgtcgccgat ttctacccct tgcatacggt
    14881 aaacacgttg tacttcacga agcaagtagt tttcaacaga taatacatcg cgaacttgca
    14941 acaattgttt tggatcgatt gatccttctg tcaatggtgc accacggtgg atcatatcgc
    15001 cttctgctac tttcatacgt gctgtataag gaacagtata cgtacgtgta tctgtttttc
    15061 ctttgattgt tacttctttg gtacgtgttg caggatcttc tgagatatcg atgacatccc
    15121 ctgttacttc tgtgatcact gcttgccctt tcggattacg tgcttcaaag atttcttgga
    15181 cacgaggaag accttgagtg atatcgtctc cggcaacccc acctgtatgg aatgtacgca
    15241 tcgtcaactg agtaccaggt tcaccgattg attgagcggc gatcgtacca actgcttctc
    15301 cgacttcaac gtcagagcca gttgcaaggt tgcgtccgta acaatgtttg cagacaccat
    15361 gttttgtatt acatgtgaag acagaacgga ttgtcactgt ttcgacacct gcatcaacga
    15421 tttgttttgc gatgtcttcg gaaatcaatt ggtttcctgc aatgatcaat tcatttgttt
    15481 caggatgacg tacttcttta cgggtgtaac gtcccactaa acgatcttct aatgattcga
    15541 tgatttcatt tccctcgcgg atggcttgga tttcaagacc tcggtcagta ccgcaatctt
    15601 cttcacggat gattacatct tgggccacgt caaccagacg acgagtcaag taacctgaat
    15661 cggcagtctt caaggctgta tcggtcatcc ctttacgagc cccgtgagtt gagataaaca
    15721 tttccaagac ggatagtcct tcacggaagt tcgaaatgat tggcaattcc atgattcgac
    15781 cattaggagc ggccatcaaa ccacgcattc cggcaagctg tgtaaagttg gaaatgttac
    15841 cacgagctcc agaatcagac atcatgaaga ttggattttt cgcttccaaa ccttccatca
    15901 gtttttgttg gatgctgtct tttgcagcat tccaaacagc aatgacacgt tcataacgtt
    15961 cgtcatctgt gatcagacca cgacggaatt gttttgtaat agtttcaact tgtttatggg
    16021 cattttcaat gatttcttgt ttttcattca gtacactgat gtcagcgatc cctactgtga
    16081 taccggcatg tgtcgaatgt ttgtacccta agtctttcat acggtcaagc atcttagaag
    16141 tttctgttac tttgaatcgt ttgaagactt ctgcgatgat gtttcctaag tttttcttct
    16201 tgaacggtaa tactaattct tgctctttga tatgtgcagg aatatcagta ccagcttcaa
    16261 caaagtattt gtccggtgtt tggacggtca agttgaagtc tgttggttca ttcagataag
    16321 ggaattcagg aggcatgatc tcgttgaaga tgatcttacc aactgttgtg atcatcgtac
    16381 gttctttttg ccattcagta aatggttttt cacctaatga gttagggttc acaccgatac
    16441 gtgaatgtaa atgaacatat ccgttgcgcc atgcgataac ggcttcgttt tgatctctga
    16501 agaccattcc ttcgccttcg cgtccttctt cttccattgt caagtagtag ttacctagaa
    16561 ccatatcttg agaaggagta acaactggtt taccatcttt agggttcaag atgttttgag
    16621 cagctaacat caacatacgt gcttctgctt gtgcttcttc attcaatgga acgtggacag
    16681 ccatttggtc tccatcgaaa tcggcattat aagcttcaca tacaagtgga tgcaaacgga
    16741 ttgcgcgtcc ttgtactaaa actggttcga atgcttggat accaagtcgg tgaagcgtag
    16801 gtgcccggtt cagcaatact gggtgttcct tgattacgtc ttctaaaaca tcccatactt
    16861 cgtcttcttg acgttcgatt ttacgtttcg catttttgat gtttgaagct agttcgcgtt
    16921 gaacaagctc acgcatcacg aatggtttga ataattcaat ggccatttct tttggaagtc
    16981 cacattggta catcttcaag aaaggtccaa caacgataac ggaacgacct gagtagtcga
    17041 cacgtttacc tagcaagttt tgacggaaac gaccttgttt ccctttcaac atatgagaaa
    17101 gagatttcaa tgggcggtta cctggtccag taactggacg tccgcgacga ccgttatcga
    17161 tcaatgcatc tactgcttct tgcagcatac gtttttcatt ttgaacgatg attccaggtg
    17221 cattcaagtc taacaaacgt ttcaaacggt tattacggtt gattacacga cggtaaagat
    17281 cattcaagtc acttgttgca aaacgtccac cttctaattg aaccattggg cgtaaatctg
    17341 gtggaataac agggataacg tccataacca tccagcttgg ttgatttcct gaagcgcgga
    17401 atgcttctaa aatatccaaa cggcggattg cacgtgtacg tttttgtcct tgtgcagatt
    17461 tcaattcttc tttcaattcg gctgcttctg catctagatc aacattatca agtaattgtt
    17521 taatcgcttc tgcacccata gcagcttgga attcttgtcc atattgttca cgtttttcgc
    17581 gatattcgcg ttctgtcaac aattgttttt tctctaaatt cgtatttcct ggttcgatga
    17641 ctacatatga agcaaaataa atgatttctt ccaaagcgcg tgggctcatg tctaatacaa
    17701 gacccatacg tgaaggaatc cctttaaagt accagatatg tgaaacagga gctgctaatt
    17761 caatatgtcc catgcgttca cggcgaactt tcgaacgagt aacttccact ccgcatcgat
    17821 cacaaacgat tcctttataa cggatacgtt tgtattttcc gcaggcacat tcccagtctt
    17881 ttgtaggacc aaaaatccgc tcacagaaca acccttcgcg ttcaggtttt aaggtacgat
    17941 agttaatcgt ctcaggtttt tttacttcac cgtaagacca gcttctgatt ttttcgggag
    18001 aagccaaacc tatttgcata ctttcgaatt tatttacatc gatcaaaagg ggttccctcc
    18061 atttcattta atggcaaacc gcaagacggc tgatcttcga ccgccttgca gctcatagac
    18121 cggattagtc taaattatct tctgcggttt caaagttttg gatgacatcg tctttttcat
    18181 cttccacttg ttcagcagct ttcttttcta attctttggc agtttgctgt tcggcaaatt
    18241 ttgtcaaagc atcaacagtg atcaaatcgt cgtcttcatc gtccatgtcg cgaagttcga
    18301 tctctgtttc ttcgatatcc aagacacgca tgtccagacc tagtgattgc aattctttca
    18361 ccaatacgcg gaatgattcc ggtacacctg gttttggaat tggttcaccc ttgacgattg
    18421 cttcgtatgt tttcacacgt ccgactacgt catctgattt gtatgtcaag atttcttgta
    18481 aagtataagc cgcaccgtaa gcttccagtg cccatacttc catttcacca aaacgttgtc
    18541 caccaaattg agctttacct cccaatggtt gttgtgtaac caatgagtaa ggtccgattg
    18601 aacgtgcatg caatttgtca tcgaccatgt gagcaagttt gatcatgtac atcacaccga
    18661 cagacacacg tccatcaaat ggttcacctg tacgtccgtc gtacagaaca gttttcgcat
    18721 cgctggccat accggcttca cgaactgttt cccatacgtc ttcatcactt gcaccatcaa
    18781 atactggcgt tgctacgtgg atacctaact gacgtgcagc catacccaag tgtaattcca
    18841 atacttgccc gatgttcata cgtgaaggca cccctaaagg attcagcatg atatcgattg
    18901 gtgttccatc aggtaggaat ggcatatctt cttccggcat aatacgggat acaacccctt
    18961 tatttccgtg acgaccggcc attttatccc cttcatggat tttacgtttt tgaacgatat
    19021 aaacacgaac taacatgttt acgcctggtg acaattcatc tccagcttca cgagtaaaga
    19081 ttttcacatc gtgaacgata ccgccgccgc catgtggtac acgtaatgat gtatcgcgga
    19141 cttcacgagc tttttcgcca aagatcgcat gcaatagacg ttcttctgca gataattctg
    19201 ttaccccttt tggcgtgact ttccctacaa gcaaatcgcc atcttggact tctgcaccaa
    19261 tacggatgat acccatttcg tctaaatctt ttaatgcgtc ttccccgaca ttagggattt
    19321 cgcgtgtgat ttcttcaggt cctaattttg tatcgcgggc ttctgattca tattcttcaa
    19381 tatggataga agtgtatacg tcatctttta ccaaacgacg gctcatgatg atcgcatctt
    19441 catagttgta cccttcccaa gtcatgaaac caactaaaac gttttggcct agagccattt
    19501 cgccttgttc catagatggt ccgtctgcca gagtatcccc tttttcgact ttctcaccca
    19561 aatgaacgat tggacgttgg ttgtagcttg ttccggagtt tgaacgacgg aattttgtta
    19621 catcgtattt atctaatgca ccgttgtcgc ggcgtacgcg gatttcagaa gcatcaacgt
    19681 attcaacgac accgtcatgt ttacataaca aagcagctcc tgagtcatgc gctgatttgt
    19741 attccatacc agtaccaacc catggtgctt gaggattgat caacggcact gcttgacgct
    19801 gcatgttggc acccatcaag gcacggttgg agtcatcgtt ttccaagaaa ggaatacatg
    19861 ctgtcgcaac ggcaactact tgtttaggtg atacgtccat gtagtctact ttatcaatag
    19921 atacttctaa gttttcactt tgagcacgag ccataacaac gtcttgagca aatgttccat
    19981 cttcattcaa tgggctgttt gcttgggcta cgatgtaatg gtcttcgata tcagctgtca
    20041 agtagtcgat ttggtcagta acacgacctg tttcacggtc tacacgacga taaggtgttt
    20101 cgataaatcc gaatttattt actttcgcat aactggacaa actattgatc aacccgatgt
    20161 ttgggccttc aggagtttcg atcggacaca tacggccata gtgggagtag tgaacgtcac
    20221 gaacttcata accggcgcgg tcacgagtca atccgccagg tcctaaggct gaaagacgac
    20281 gtttgtgtgt caattcgcca agagggtttg tttgatccat gaactgtgac aactgagaag
    20341 aaccaaagaa ttctttgata cttgccacaa ctggacggat attgatcaat tgttgaggtg
    20401 tcaatgtttc agtatcttgg atcgacattc tttcacgaac cacacgttcc atacgtgcta
    20461 gcccgatacg gaattggttt tgtaaaagtt caccgactga acggatacga cgatttccta
    20521 agtggtcgat atcatcgaca ttgccgatac cttccatcaa gttcaagaaa tagctcattg
    20581 aagcaatgat gtcggctgga cgaaccgttt ttacagctgc ttctggatag ccgtttccga
    20641 tcacgtttac ttcacgttca gggtctcttg gtgaaaatac tttgatgact tggacagtca
    20701 ttggatctgt gaccacaccg tcttctgatg gatagtaagt cactgaattt aggccattat
    20761 caatgaaagg agctagtgtt tccatgactt ggtgagtcag aactgttcct ttttcaacga
    20821 tgatctcgcc tgtttctgga tcaactaatg tttcagctaa tgtcagattc aatagacgtg
    20881 ttttcaaatc taattttttg tttactttgt aacgaccaac gtttgctaag tcgtaacgtt
    20941 ttggatcgaa gaaacgagca ttcagcaagt tacgtgagct gtctgctgtt tttggttcgc
    21001 ctggacgcaa acgttcgtaa acgtctttca aaccttcttc tgttcttgag tcgcttgcgt
    21061 ttttatgcaa gtctttttcg actgtattgc gtaatgtttc gctgtcgccg aagatttcaa
    21121 agatcgtatc atcggaacca aatcctaacg cacgaactaa tacggttaaa ggaatcttac
    21181 gtgtacggtc gatacgtaca taagagatgt cttttgcatc agtttccatt tctagccaag
    21241 ctccacggtt agggataaca gtagaaccga atccttcttt tccgtttttg tcgacttttc
    21301 catggaaata gacacctggt gaacggacta actgagaaac gattactcgc tctgccccgt
    21361 tgatgatgaa tgttccttgt tctgtcatta atgggaaatc accgaagaat acttcttgtg
    21421 ctttgatttc gccagtctcg cggtttgtca aacgcaatgt tacatgtaat ggtgcagagt
    21481 agtttgcatc atgcgcacga gcttctgcta ctgtatactt tggttctttc aattcataat
    21541 ctacaaattc caaagataga ttgccattga agtcatcgat tggcaaaatg tcttcaaaca
    21601 tttctctaag tccctcatct aagaaccatt ggtaagagtc tgtttgaatt tcgatcaaat
    21661 ttggtaattc caacacttca ctgattcttg cgaaacttct acgttcgcga tgttttccgt
    21721 attttactac gtgtccagcc aagctcttca cccctctaaa atgtcattca aaaagactac
    21781 ccgatcaatt gttacaataa tatttcaaat attattttta ggtaacaaca gacatttttt
    21841 gcggctttta ggcaaaaaaa aaccaaaaat agaacattct cttgaaacct tacttcgtga
    21901 gcttctactt ttgtttcctt atattaaagc cgaaacggac aatatttcgt ttctgccttt
    21961 tcttcatcag atagtttttg caacttcata tactacactt attttctata aaagtcaaca
    22021 cttcctggcg tttcttctgc tttttcgcaa cattttctta atgttttcaa ttttatgttt
    22081 tcaattatct gacaattctt aattgacaga cctttttatc tcggttatac tttagaaaat
    22141 tcattttgtg gaggaacttc tatgcgaacc aacatcaacc aaatcgcacc aacacaactg
    22201 aataactttt actttagcta ctttagatag gtttgtattc atcatgatgg atacaaacgt
    22261 taaacgacag tttgtatcta tgatggctaa agaactttgt attgcagcaa aaagccacgt
    22321 agacaaaatg ctaagtggct gagcagtgaa aatacaaagt aaaagtatca agcattctac
    22381 tttttcaatt tattttccga cttttcaatc catttggaca ttttgtctga atggattttt
    22441 tgtttcccaa aattaaggag gaaaaagaaa tgaaaaaaaa tataatcatc ggattaggcg
    22501 ctgccctgct actagcttta ggaggttgcg cacaaaaaga caattccgca gaggcatcag
    22561 atggacaaaa aacaattggc gtattgcaac ttgtacaaca taactcgtta gatgctgcct
    22621 ataaaggatt caaagaagga ttagcagaag aagggtataa agaaggcgat aatctaacaa
    22681 tcgattatca aaatgcccaa aacaatcaag acaacctgaa aagtatgagt gagaaactcg
    22741 tcaaagaaga tcctgatttg ctacttggaa tcgccactcc agcagcacaa tctttattaa
    22801 atgaaacaac cactatcccg atgactgtca cagcggtcac agacttggaa caagcccatt
    22861 tagtcacatc caatgaaaaa cctggcggca atgtgactgg cacgacagat atggtgccaa
    22921 tcgaaaaaca aatcggctta ctgttaaata tcgtgccaga cgccaaaaca atcggcatca
    22981 tgtataacaa cggtgaagca aactctaaga tccaagctga tttggcagaa aaagaattga
    23041 aaaaagcggg cgtcaaagtc aaagtactga cagcaaattc cacaaatgat gtccaacaag
    23101 tgacgaaaag tctagctaaa gatgtagatg gaatctatat tcctactgat aacacgttcg
    23161 caagtgcggc aagtgttgtc ggtgaagtcg caaaagaaac aaaaacacct gtcgttgctg
    23221 gcgcagttga tcaagtcaaa gacggcggtc tagcaacagt aggaatcgac tacgaaagct
    23281 tgggcaaaca aacagggatc atggcagcaa aaatcttaga cggcaagaat ccaagtgaac
    23341 ttcctgtcga aacagcgaat gatttggaac tgtatgtcaa taaagacatg gccaaagcat
    23401 taggcatcaa tccagacagc atcaaagaag ctgaataaaa cacaggagga ttctcatgac
    23461 aaatttgctc tctcttttct tatcatctgc atcgcaaggg gtcttatggg ccctacttgc
    23521 gattggtgta tacgtcactt tccgtatctt ggacattgcc gacttgactg ctgaaggaag
    23581 ctttccttta ggtgcaggga tcgcggcagt aagtattaca aatggctatc atccattgac
    23641 agcttgtctt ctaggctttt tagggggtgc ggcagcaggg cttgtttccg gactgcttca
    23701 cacaaaattg aaaattcccg cccttcttgc agggatcatc accatgactg gtttatattc
    23761 tgtcacatcc cgtgtgatgg gtgcgccgaa catctcttta ttgggtcagc caacgatctt
    23821 tcattgggtc gagtcattcg gcataacaaa agacaacgct gtactgatcg tcggtctgct
    23881 ttttgctttg atcgttgtat gtttacttgt tttgttctta cgtacagaaa caggacttgc
    23941 tattcgtgca actggcgata atctggcaat gagcgaagca aacgggatca acacagataa
    24001 tatgaagatc atcggttata tgatttctaa tggatgcatt gctctttctg gctccttact
    24061 tgcgcaaaac aacggctttg ctgatttaaa ttctggtatt ggaacgatcg taattggact
    24121 tgcttctatt atcatcgcag aagttttgtt acgtaaccag ccactatttc tccgtctctt
    24181 gactgtcgtt ttaggtgcgg tcatttatcg ttttatttta gcgttagtct tcgaactgaa
    24241 tgttgaacca agtgattcga aacttgcttc tgctttcgtg ctagtgatct gtctttcttt
    24301 cccgcagatc cagcaaaaat tccatttacc tcaaaaaaag aaaggcggta gtgtcaaatg
    24361 aatgacgtct taacattgga aaatctccat catacctttg aaaaaggaac gatcaacgaa
    24421 aatcatgtcc tgaaaggcat taatctttcc ttagatcccg gcgacttcgt gacgatcatc
    24481 ggtggaaatg gggctggtaa atctactttg ctgaacagca tcgccggtac attttctgtc
    24541 gatcagggtc aaattttatt gaatggaaaa aatatcacga aaaaatctgt tgtcgaacgt
    24601 tcgaaatcca tcagtcgcgt attccaagat cctaaactag ggacagccgt tcgtttaacg
    24661 gtagaagaaa atcttgctct tgctatgaaa cgaggaaaaa aacgcggctt cttccgtggt
    24721 gtcaaaccac aagaccgaac atttttcaaa gaccagctgg ttcggctaaa tttggggttg
    24781 gaaaatcgtt tatcctcaga aatcggtttg ctgtctggag gacaacgtca agctattacg
    24841 ttattgatgg ccacattaca gcgtccagaa ctgatcttat tagatgaaca cactgcagcg
    24901 cttgaccctg ctacttccaa aacggtgatg gaattgacag aaaagctgat cactgagcag
    24961 aatctcactg cttttatggt cactcatgac atggaagacg cgatccgcta tgggagtcga
    25021 ctgatcatgc tgcatcaagg aaggatcgtc gtagatatcc agcaagagga aaaacagcag
    25081 ctcacagtgc cccaattatt agcgttgttc aaacaaaaca gcggcacggc attaaaagac
    25141 gatcaagtac ttttatcttc ataatcagaa agaggatggg acaaaaaatg aacgcccacc
    25201 ctcttctttt ttctctttcc ttctttcgtt tttgctttat gtatcaaccg ttgtttcttc
    25261 gaaaactatt ttgttctctc cttcttcctg atagaatgga tgggaaagga gcttgatgcc
    25321 atgacaacga aagacaaggt acttcaatta ttgaaaaaga caccagattt tctttccgga
    25381 gaaaagttgg cacagcaact ggaagtttcc cggacaagta tttggaaagc catcaaagaa
    25441 ttggaaaagg caggctatcg ttttgagcac gggcctacag ggtatcgtta cctgccctct
    25501 gacgtactag atgcaaaaga aatccagcaa gagctacggt cgtttattcc tgatttgaaa
    25561 gtacaaacga tgttgacttc tgaatctacg atgaaagatg ccaaactggc agcagctcaa
    25621 ggagattctg tttcgaagtt atttgttgct gatacacaag aacaagctcg tggacgtttt
    25681 ggacggcctt ttttcgctga acctggacga ggaatctaca tgagtctctt gctccatcca
    25741 gacaaacggt ttgatgaatt gcctcaatat acgatcatcg cagccagtgc ctgtgtgaaa
    25801 gccattgaat ctgtaaccgg tcgttctgct tcgatcaaat gggtcaatga tatctacatg
    25861 gatggaaaaa aagtctgtgg gattttatct gaagcagtca gtgatatgga aagcggcagg
    25921 atcagtcata tcatcattgg gatcgggctt aatttttctg ttcctcaaaa tcattttcca
    25981 gctgatctcc aagaaaaagc catctctgtc tttcccgacg gacaagctag tgtcacaagg
    26041 aatcagctaa tcagcaaaat ctggcaagaa ttgttcactt atctttctca cccagaaaaa
    26101 gcgttggact tctataaaga aaaatcattc gtattgggaa aaaccgtgtc gttcaatcaa
    26161 aaaggaacga cttatgtcgg tcttgcaaaa gaaatcggtg atcatggcga actgatcgtc
    26221 caaacaaatg aacagaagat gagtttgtct tctggcgaaa tcagtctcac atctatccgt
    26281 acgcaataat tatcagattt cacaaaagga cacctgatct catacagtgt ccttttttct
    26341 gaattctctt caaaagactc tccttattct atcagctgac agtttctcta tcctctagaa
    26401 aaaggtattc taaaaatatc tataaagcat ggtacacttt ctttaaatga atcaatgcaa
    26461 aaaacaagag agtcatcagt ttacacgatc aaaagagata gcaggccgct tcctttttca
    26521 atcttataga tagaccatgg gtaacgataa aaacaaaaaa gaacttgcga cttattttgc
    26581 cgtaagttct tttttagcta gtgccttaat ctcttaatcc caacaaacgt ctcgcataga
    26641 tcacctgacc agagtgcata gcagcatcat caataataga aacgagacgc attcctcgtg
    26701 tgactggtgg tgtccagttc tcgtcaatta cttcatctag ctcggaaggt tccagccttc
    26761 ttaagtaaga aatagcaaaa tcagcagctc ctcgcaagta tcctagcaga tcttctattt
    26821 gattagcttg gatctgcttt gcttgttcca gactatgctt ccaatcttgt tcgttatctg
    26881 ctatcgtcat cggaaaccgg ctttcccagt cttgactatg ccaaattgga gaagttcctg
    26941 ctaaatcagc aatctggaaa tctaattctc gagcggtatg ccaagccagc caagtcattg
    27001 atttgatctg cggggctcta tcttccactg gaaaacgatt ggcctcctca atagagacgc
    27061 cttcaaacaa acgttcaaac cgatcgtatg cccgatccac actctcgcat agcatatcta
    27121 aataatccat ttattttcct cctttttatt tatcctactt actttttcct aattctagga
    27181 tacctactta tatgttacta cacaccctta ttttttacac tttaacagtc accatcatca
    27241 atagtttgga gattgaaaga aaaagatagt gtcgaactat caattgaaca agaagcaaca
    27301 atatgaacgt ttcattaata ttctttaaat aaacaatgat ttttcaaacg aacagtttaa
    27361 gactaggaga aaaagagtat aataggaaaa tgtttgaaag gaaaggaagg ggaaaaatga
    27421 aaaaaagggc aagtttacta gtcctagttg ctttgttgtt caatatcttt tcgtatggtg
    27481 tggccgtttc agcggaaaca tacattccta aagaccctgg gaatatttat aatgatctaa
    27541 ctggtaacta tggcttgtta ggaattgcta gtcaattcca cgtatttgct aaaaataaga
    27601 caactgtgaa tgcacataca gacggaaata tagcaacaca tgaacttgat gcaaaaaata
    27661 attttggtac agagcttcat gaaggtttat tgaaccaaga aataaattat atacaaaaaa
    27721 ctgattcttt cgctaattct tcaggcatac ctacaggtag cacaagaatg aataaatttg
    27781 tcgttggcag taacgtggat gtagcacttc aagatggtca tcctacagtc aatggatcca
    27841 ggcttgatca cttaactcca gatgattttt ataaagatcg tgctgggcat gaatatattg
    27901 atttcgagca ggaatttgct aaattgaatg tagcagcaaa tgatcttgca acgatcactc
    27961 cagctaaaac atatactgct gcagattttc cagacatgaa caatcggaca atcgatttaa
    28021 ctgatttatc agataccggg tttttattgg taaatatcga tgcagaagtg ttaacgatga
    28081 atacgccact tcaaatcatt aatccaaatg atcaagtagt tgtatttaat gtcatcaaca
    28141 gttcttctgc attgaacgta cagtcaccaa tcaaatacaa tgatcgttca aatcatgaaa
    28201 cagaagattt ttcggatgcg aatatctctt ggaattttgg aaatgagatg actgatttga
    28261 caatcagtgc tccttttcaa ggaacaatcc ttgcgcctaa tgctactatc cgcattactc
    28321 aaaaccaaga cggaacgatt ataggaacaa atgtgatttt agatgcagct accaatcgtt
    28381 gggatcctaa cgaaatattt atcagcgaca atggtacaga tacgacagac acaagcgaca
    28441 gtacagatac atctaccaca agtgatagct cagattcatc aacttcaagc gacagtacag
    28501 atactacctc taccacaagt gatagcacgg atacatcagc ttcaagcgac agtacagata
    28561 ctacctctac cacaagtgat agcacggaca catcagcttc aagtgacagt acagatacta
    28621 cctctaccac aagtgacagc acggacacgt cagcttcaag cgacagtaca gatactacct
    28681 ctaccacaag tgatagcacg gacacatcag cttcaagtga cagtacagat actacctcta
    28741 ccacaagtga tagcacggac acgtcagctt caagcgacag tacggataca tctaccacaa
    28801 caagtgatag cacggacacg tcagcttcaa gtgacagtac agatactacc tctaccacaa
    28861 gtgatagctc agattcatct accactacaa gtgatagcac ggacacgtca gcttcaagtg
    28921 acagtacaga tacatcctct accacaagtg atagctcaga ttcatcaact tcaagcgata
    28981 gtacagacac gtcgtctacc acaagcgata cacgtcatgc aagtgtctcc ttctcaaaaa
    29041 caagttcgga taacaggacg aactttaact taactaataa aacaagcaat gacaatggaa
    29101 acaaatcaaa aagagcactt cctaaaactg gaagtcaatc gaataattgg ataacactcg
    29161 caggcgttat attacttgta atcggtttaa gaatcacttt tatgagttat aagaaatcga
    29221 aaaggtaaaa aaatattcaa gataaaaaag agcaacgtcg ctaacggacg ttgctcttta
    29281 tttttgcttt ttatcataaa tacaagaact acaattcaat ttgtcaaaaa acacgtagat
    29341 ttccttctga gaaatttatt tacgtatcga acctttccct attcagtctt tgcgatccaa
    29401 aatcagttgg gtaagggaaa agagattctc ttttgttcga agagaggtgg caggtaattt
    29461 ctttatttcc ttcaaagcct tttctgtgta agaggccgct aacttcctag cttcttccac
    29521 gcctcctgta tgttcaacga ttttgcgtac ggcgtcgaca tcttcttctg tcattgccgc
    29581 tttcttctct aaataaggta gtagctttgg tttgtttttt tgcaaagagt agatcaacgg
    29641 taaagaatag accccttgac gcatgtcttc tagtacaggt ttgccgattt cctggctctc
    29701 ttttgtataa tctaaaatat catcgataat ctgaaaggcc atcccgatag aaagtccgat
    29761 atctcctgct ttttttgcga agcgctcgct tgttccactt tcaaaagcgc caacagaaca
    29821 acttaattgg aacagttcag ctgtcttacc tgaaatattt tccaaatatt cttttacaga
    29881 aagttcataa ttatagcgat catccatctg ccctaattcg ccattcaata ctttttccat
    29941 actgcgagaa ttcaattgga gacttttcaa ggaagtggag taatcagaca atagtttaaa
    30001 acagcagaca aataaatagt ctcctgcata gacagaaacg acattaccaa actgactacg
    30061 gatattaggc tgaccacgtc gcagatccgc ttcatcaacg atatcatcat ggatcagtgt
    30121 ggccgtatgg agcatttcga tagaagctgc caaagcgatg gctttgtctc gatcttgctc
    30181 tggtccaaat tgtgagaaca atagctgata ggcaggacgc aacagctttc cgccggcatg
    30241 gatcgtttct ttgatggctt gttcgacagg tttgtttttc aggcggacag tttcttccat
    30301 ccgttttagt gtttctgtca attctttact taaagcagga tagttttccc acattggatg
    30361 tagcttcata aaaaaactcc ttgtgtgacg attatttttt ggtaaacatt gggctccatt
    30421 tttgtttggt tccctgctca taatcttttt ggaatcgttg aagtgtctgc acatgtcgta
    30481 aaagataatt cgctgcgatc ccgattgcaa tccctgataa aatccctaaa actgaaagga
    30541 ctggcaagta caacatgact gtccatgatt gtgcaatgaa agaagccatc aaaagctgtc
    30601 cgacattatg catgaaaccg ccggccgcac taattccgat gatactgaca cgttttggtc
    30661 ctaattgctt gaccaataac attccaaaat aactaagcaa agcaccactc atactataaa
    30721 ggaatgtgga aatcgttccg cctagcaaag tagtcaggaa tagtcgtagc caaagtagta
    30781 agaagctgtc ttgttttttc atggtaaaaa tggcaacgat cgtgattaga ttggctaagc
    30841 ctagttttgc accaggcgca aaagaaaaag gataagggat catattttcc aataaaccaa
    30901 tgatcactcc ttgtgcaaca agtagcgaaa tataaatcat tttttgtaat ttactcatgg
    30961 tgtgtgaagg tctaataaat cagactgtct tcgtcacctc catctgttga acggacttcg
    31021 atcactaatt tatgtggcaa acagacgatg gtttcgccat ttttcgttgc ccagcctcga
    31081 cgtacgcaga tctggtcatc gcaatctgct tcttttatcc gtactcgatc gccatctatc
    31141 tcgatcaagt tataatctcc gtgttcatct ttgtaagtat atgtgttgct ctcttctcca
    31201 gccatcaaag ggaatgtttt gatttctgtg ccatccacac gcagtaccgc ttctttttct
    31261 gctgttgcgg tgttttgctg aagaccaaaa atcatgactg gtaaaaaaga aagaacagtc
    31321 aaaagcaaaa taataaaaac atcccaaggt ttgagcagac ttttttcagc aaattcttta
    31381 aggctcattt ttgttctcct ttttccagat taactcaccc aattttagca taaaaacaag
    31441 aggttgtgac acctgtgtct tgctttgagt aataagaacg aagaatagaa aacagctttc
    31501 catgttttct aattctttgg gcttattacc gagaagcagc aggtggaaca ccgtttataa
    31561 caagaggttg tgacagaagt gggcaacttc aagaaataag aaggaattta cgaaaattac
    31621 ttttcaagtt tttgtgaaat ttcggcttat ttttcgaaga agctacttct ggaacaccgt
    31681 ttataacaag aggttgtaac agaggttgtg acacctgtgt cttgctcatg gaggcttatc
    31741 tcgggcatct tattccttta attttacata aaaaggaggc tgtgacggtt tgtctcagcc
    31801 cctttctatt gaaatcttgc attcaaataa cgttctttat ctatttattt cttgccgtac
    31861 tgggaacttg gtctaccgta ccaccaatta cctagtgtac gttgtagcca tggaattacc
    31921 cagcgatcca acccaatcgc acgtccggaa ccgttcatca atgcgaaagc aacaaaaatg
    31981 aaccaaatgt ttacccagta aaacatgcct gataagcaga acgtgatcac caatacaatc
    32041 gttgtcgcac tgcatagcca tgtgaataat ccagcaatca atgctaatgc caaggcaatt
    32101 tcaaagaata ccatgaattt ttggaagaac attgctacat cattgtttgg catgatcact
    32161 tgcatgatct ttgtgaacca gtctggtgac ttctcaatca cagccattgg ttcttcaccg
    32221 tatgcataac tcaaaccaaa atgtgcagct tgagtcgctg cttctccggc acttgcccct
    32281 gctccgctgg cagctgtggt tgcatcacca gcagcagaag caccactggc aacatctgtt
    32341 acattgtatt cccatgtcca agggaacaca ctgtttccag tgaaccatga tccttcacca
    32401 aaccaagtac ttggtttgaa cacttcacca ttcccaatga tttttttgga agcttcaacg
    32461 aaccaaacta aaccatagaa aatacgaagt ggcacactcc ataagacatt gccgtaacga
    32521 gaagaatgtc ctcgagtcac gttgcgatca tctttgatat ggaaaaactc atgcattaaa
    32581 tattggaaca tataataacc agaacgaata tcaaagaagt atttcaaatt cacgatatgt
    32641 ttcattacga tagctaaaaa tccgcttaaa tggatcttat caaataggtt agctaccccc
    32701 catttggcac caatagaaac cataaatcct tgatagtttc ctttaaattt atgcttttct
    32761 ccgcctttga tcgaagccac gatattcgct gcggctgtgt gtcctgtttg ttcagcagct
    32821 tgaacgatct gcggtgtcgg tgttcctggg aattcttcat aaaagactaa atcaccaatg
    32881 atatagatgt ttttatcttc gtagcctttt gcctgcatat attcattggc gatcaaccgc
    32941 tgtccgcgtg ctgattctaa tccaaagtca gcagcgtcag aagttccttt gacaccagct
    33001 gtccaaatca gtgtgtgagt cggaatgttg cgtccgtctt tcaatttgat atgatcagca
    33061 gccacttcta cgattggtgc attcaataat aattccacgt tgtgcttacg caaatagcgt
    33121 tcagcttttg cagcatcatt tcgatccaac atattcaaga tcgtcggcat tgcctctacg
    33181 accatcagtg tgatttcgtt tggatctaat ttataatctt tggctaggcg atttttccag
    33241 tcgatcagtt ccccaatcat ttcaataccg gtaaatccag aaccacagac aacgaaagtc
    33301 agcatggctt tacgcagttc atcatctggt tccaatgcag cttttgctac tgtcttctca
    33361 atatgttccc gaatccttac agaatcttca aatgaccaaa gggtgaatcc gtgtgctttg
    33421 acaccaggtg taccaaagtc attcggctct ccgcccattc caaggatcag ctgatcaaaa
    33481 ggataggaac ctaatttggt cttgacgatt tttcgttctt tatcgatacc gattacagtg
    33541 tctgtgacaa gttgtacatt cttttgacga gcaaacaaat gctgcagatc gtattggatt
    33601 gcttctggtt ctacacgtcc gccagctact tcatgcaact cggtcatcat agtatggtac
    33661 gaatgacgat cgatcagagt aattgctaca tcttcttttt tcagtttctt tgccaagtat
    33721 tttgtagctg aaacaccggc atatcctgct cctacaacga caatattttg ctttgccatt
    33781 gatcatccgc tccttataga ctatctatat aatgaatata tgataattct tttagtttga
    33841 tacttattac acaatctcca tatattatat agatgttctg ttcttttgtc taactccttt
    33901 tggatttttt tcaaatttta aaataaagcg ttttttcacg aaatttgctg aaagaaaacg
    33961 tttgaaatat tacatgggtt atttatcaaa aatagttgac ttataggcta aaaagattag
    34021 aatgtataat gaatcacaaa gaaaaattgt ggactagaaa tggaatggtg gggtattaaa
    34081 atgaagaaaa aacgttttgt aactggattt gccgtattag cattttcagc tctagtgtta
    34141 ggcgcttgcg gcgcagatga caacggaagc tcaaactctt cttctgaatc atctacagca
    34201 caatcttcga ctgcaaaaag cacaacagaa tcatctgcaa aagttgtagc tggcggagat
    34261 ttgcaagacg gcacatataa attagaagaa aaaaattatt caaatggtta ccgtgcagtc
    34321 tttgaaatgg ttgtaaaaga cggcaaaatc actgaatcaa aatatgataa cgtcaatgaa
    34381 aacggcgaat caaaaacaaa agatgcagaa tacaacaaaa acatggaagc aaaatctggc
    34441 acaaacccag aaaaatacat tcctgagttg aatgaccaat tcttaaaagc acaaagcgca
    34501 agcggtgtgg aagtcgtaac tggagctact cactcatctg aaagcttcca aaactacgca
    34561 caacaattga tccaagctgc tcaagctggt aacacagata caatcgaaat cgacaatggt
    34621 gctgatttga aagacggcac ttacaagctt gaagaaaaga actactctaa cggctaccgt
    34681 gtgcaattcg aaatgacagt tgctggcggc aaagtaactg aatcaaactt tgattatatc
    34741 gacaaagacg gtaaatcaaa acaagatgat acagaatata atgaaaatat gaaagcaaaa
    34801 agcggtacag aaccaaaaac ttacatccct actctgaacg acgaattcgt caaagcaatg
    34861 ggcgaagaag atggctcacc tgctaatgta gaagttgtta caggtgctac acacagctca
    34921 cattcattca tcatgtacgc acaacaatta gtgaatgcag ctgaaaaagg cgacactcaa
    34981 acaatcgaag tagataatat cgtaacagaa aaataatcac ataatcatac taataaaagt
    35041 tggagggtgg gacgatagcc agattaggca gtcgtctcac ctttcaaaat gaagagagga
    35101 aaaagatgcg gaaaaaacta ctattttcct taatcacact gggcatctct tttatggtgc
    35161 taactggctg cagcaactca gcaaataaaa caaccactga aagttcagca gctaccaaga
    35221 tattgaaaga tccatattct gatgaacagt tcttgttagg gacttacgtt cgtatccgtg
    35281 tgtatgatga aggcaaagaa tcagcattga aacctgcctt cgatcgagta aaagaacttg
    35341 gtgataaaat cacgatcaat caaaaaggtt ctgagatcga tgaagtgaat gagcaagctg
    35401 ggatcaaacc agtaaaagtt tctgatgacg tctacacgtt agtcaagcgg gcctacgaat
    35461 atagccaaga ttctcaaggc ggattcgata tggcgattgg tgcaatcaca caattatggc
    35521 gaatcggttt tgatgacgcc agaaaacctt cccaagaaga aatcgatcag gcattgaaat
    35581 tagtggatta tcataagatc gaactaaatg acaaagaaaa aactgtttat ctaaaagaaa
    35641 aaggaatgat catcgattta ggtgcaatcg ccaaaggtta cattacggac gaagtagtga
    35701 aagtcttgaa aaaacaagga gtgaccactg ccatcgttga cttgggcggt aatgtctatg
    35761 tcttaggcca cagcccaaga ggcgaaaacc aagactggac agtcggcatc caagatccga
    35821 acatggctcg cggatctgtt ttaggttcta ttaaagaacg gaacaaaaca ttagttacct
    35881 ctggaattta cgagcgttat ttagaagtag atggcaaaaa ataccatcat ttgtttgatc
    35941 caaaaacggg ttatccgttc gacaatgata tcgcaagtgt cacgatcata acagataaat
    36001 caattgatgg tgacgggttg tctacagcag tcttttcaat gggtgtgaaa aaaggactgg
    36061 aatacgtcga aagcgaattg aataacggga cggaagcgat cttcgtgaca aaagacgata
    36121 aagtatatgt gaccgatcca atcaaagaca ccttcaaatt atcggaggac tcccattaca
    36181 cgatgggtga ccgttctgaa ctaaaataag gagtacgaac catgacacta aaaatctttt
    36241 tagaagtagt tgaaatacga acaaaggtgg ctagtgtatt tccttttgcg gtaggggtat
    36301 tattctctat cgcttttttt catgagatac attggctaaa cactttttta ttttttattg
    36361 gaatgctagt ttttgattta gcgactaccg ctatcaataa ttacatggat tatgaaaagg
    36421 ctcattctga agtatataag tatgaagaaa atgtcattgg ccgcagaaat ctatctcctg
    36481 tattgattcg aaacatgatt ttagctatga ttgcatttgt ttttgttatc ggcgttattc
    36541 taacgatcaa tacgggttgg cttttacttt tgatgggatt tatctgttgt tttattggag
    36601 tattctatac attcggaccg attcctttat ctcgaatgcc tttaggggaa attttcagtg
    36661 gggtgactat gggccttgga atttttgcca tggtcgttta tgtcaataca ttcggttcag
    36721 atgtctatga tctgacagtc aacttttcat ctggacaatt tgatttggta ggaaatcttt
    36781 gggctatagc agcaatcgta ttagcttcct tacccttggt gtttacgatt gccaatatca
    36841 tgttggctaa taatttgcgg gatcttgacc gtgatattga aaatcatcgc tatacactag
    36901 ttttttatat cggacgtcct gcgggaacag tcttattcca gctattgatg tatgcttgct
    36961 atttcgtgat tttaatgggg ttgttgagcg gtatctatga atggccgatc ttggtcacct
    37021 tcttgacctt gccaattgtg aagaagcatt tggatcaatt caagcaaacg ttaccccagc
    37081 ctatcagttt cagctattcc atcaaaaata tggtcttatt caatgccagt tatgtattgg
    37141 ggctattact aacaatcatt atgcaagcca tttaacagca tgaagaggtt gtgaaaaaac
    37201 tgccctgcat caagtaataa gaacagccaa ataaaaatag ctccttatat ttttcgtttg
    37261 gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttatcaggag gttgtgaaaa
    37321 agtcgcttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca catttttgca
    37381 taaatttggc ttaataccga gaaactggct tttgaacacc gtttatcaga ggttgtgaaa
    37441 aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc
    37501 ataaatttgg cttaataccg agaaactggc ttttgaacac cgtttatcag aggttgtaaa
    37561 atcagtgttt tgtcctgagt attagagaaa caaatggaaa aatagttcct catattttgc
    37621 cgttttttga gctaataccg aaggacagct gattgaacac tatttgttcg gaaaaagtga
    37681 ctaactatga cacaattttt ttcacagtcc cttttttcac ttgatttgat tcgtaccagc
    37741 gaacttgacg agaactttct tggtttggta actatatggt attcttttaa catcctcgtt
    37801 tgcttcttct aatttattta tacaaagtca cactttttta aacatctgtt cttcgataca
    37861 tttctatttc ttttaaacat cgataccaat attttgaact taagagtgca taccaaatgg
    37921 attcatttac tcttcgtcag ttacgaagat aatcaaaatc tccaacttcc ctacttattt
    37981 cgttttgaga attatcgaag caatcttaca taaataacta aaaaacaaaa aaccagaaaa
    38041 tcagtatact gattttctgg ttttacatgg tttgttcaat aaaaagctgt tggaactgtt
    38101 aatattaaat tatcgcatcg ttacgaattc ttccgctcct gttggatgga tggctactgt
    38161 attgtcaaag tcttctttag ttgcacccat cttgatagcc acggcaaaac cttgcagcat
    38221 ctcatcgaca ccaacaccaa tagcatgtaa gccgacgatt ctttcttctt ttccgacaca
    38281 gatcaatttc atttcgcatt tttggcgata atcatttaat gcaaagtaca ttggggtaaa
    38341 gcgtgaacga taaactttca attcattttc gccatatttt tccaatgctt cttcttctgt
    38401 caaaccaatt gttgcaattg gtggatgcgt aaagacgact gtcggaacaa gtttatagtc
    38461 aagataagaa tctttctttc cattaaataa acgttctgat aaacgtctac cagctgcgat
    38521 tgcaacagga gtcagatcta atttaccgat cacatcacca acagcaaaaa tatgttcatc
    38581 cgttgtattt tggtatttat ctactttgat aaaccctttt ttgtccaatt ccacatttgt
    38641 attttccaga cctaattttt cggtattcgg tacacgtcca ccggcaaaaa gtacacaatc
    38701 accagttaag gtttcaccat tttcaaatag caccgtatat tcgtcattgt ttttagtaat
    38761 ttctttagca atatgatgtg cgtaagtttt tattccagct tcttggtaca tttggatcaa
    38821 attgtccgaa agcattttat caaacgttct aagtggtcgt tcttttcgat atgcccacat
    38881 gacttcacta cctaaaccac gaaatacacc tgaaagttca gccgcaatgt atccagcacc
    38941 taaaacgatt gctctcttag gtaactctgt taattcaaaa aatccatttg aatcaattgc
    39001 gtattctcca ccaggaatgg tcattttact tgcacggcct ccagtagcga tcaagatttt
    39061 aggagcacga taggtttcac cgtttacttc gatcgtttga ctatcgataa aagtagcgta
    39121 accttttata gcctcgacat gattgctatc taatcctctt tgataagcac cgtgtagaaa
    39181 atcaatatat ttttctcgtc tttctactag accggcgaaa tcaacatttt ttaaatctgc
    39241 ttggataccg taacttgagg catctcgttc aattgtttcc aaaatcgtac ttgcttgcca
    39301 cataactttt ttaggtacac aaccgacgtt gacacatgtt ccgcccaatt cattcgcttc
    39361 aatcagcaaa acttttgcgc catgcatccc tgctctgttt gctgaagcaa taccgccaga
    39421 gccgccacca attacgatgt aatcatatat ttgcattttg ttcctcctta ttgaagagat
    39481 caatcaaaac ctttatttaa cgatcctttt tctacagaaa acaagtgtaa cacttttctt
    39541 gaattttgta tgcgaagatg cttaaaacag ggatatatca gctttttcct tatttacgtt
    39601 ttcggatcat atctccaggt ttcaccggct gtgcgactgg catagttaaa atcatcattg
    39661 gatttggtgc tcggtcaatg gattcgcctt cttcattatg cataacttca acggtttgac
    39721 tgaaatgact gaatcctgga ccataaaatt cgatttcatc tcctacagaa aacaagtttc
    39781 tctgacgtat cgtagcaatc tgtgttgcct catcataaga taatacttca ccgacaaatt
    39841 tatatactgg gattttacga cgttcaccga ataattgttc gttttcagtt ggtgtattgt
    39901 agtaaaaacc tgtagctaat tctcgttgtg ctactttcca taactcgtcg atccattctt
    39961 gcttgcagac atagttttca ggatcttcca tataactgtc tacagccgct ttatagacat
    40021 ttgacaccgt agatacataa tggatcgatt tcattcttcc ttcgatcttg aagctgtcta
    40081 cgccgttttc aatcaattct ggaatatgtt cgatcattga catatccact gcactcatcg
    40141 agaattcttc ttccacttcg cctgtatcag ttaaactgtt tcgttcttga ccaaaaggca
    40201 tatcgaacaa tccatacttc cagcgacaag attgagaaca tccgccacgg tttgcatccc
    40261 gcatcgacat atggttggac aatgtgcatc gaccagaata agagatacac atggcaccat
    40321 ggataaatgc ttcgatttcg gtatccgtat ttttacgaat ctctgccact tcctccattg
    40381 aaacttcacg agctaaaacg acacgttcca acccttcttc tttccagaat tccaatgtct
    40441 caaaattagt tgctgaagct tgagtggata aatggatcgg cagacctggg gcttctgtcg
    40501 cacaaatttc gatcaaagct ggatcagaaa caatcactgc agaaataccg acatcacgca
    40561 aactgcggaa aaattcccct gctccatctt catttccttc atgggtgacc atattagcag
    40621 caacgtatac cttcgcatca tgttcttttg caaaagcaac tccttcagcc atttcttcat
    40681 aagtaaaatt acctgcacgg cttcttaaac catatgcatt cccgccaata tatactgcat
    40741 ctgctccata atggatcgcg gtttttaact tttccagtgt tcctgctggc gctaggattt
    40801 ctggtcgttt caattttctt tttactgtca tttctttcat catactcctt atttgatagc
    40861 agaaggatca atataaaaga atcctgtatc taattcacgg ttttcaggat gcaatttact
    40921 gatttcgcta gtcatttttt ccgctttttc ttgatcccag ctaccttcta aaatttgttt
    40981 ttttgcttcg gcgaatagtc gagtgatgtt tacgaatttt tcgcctggag taaataatcc
    41041 gtccaatttc catgtacagt aatgatgctg ataaagttca ttcaattcat tcgagagatt
    41101 cagatcattg ctggcaaaaa tatgtgtgcc atgactatct tcataaatag aataatgtgt
    41161 ctcttctttt ttaggttccg agataaaaag acctctttca cgatcttttt gttcgtcttg
    41221 tttcgtatag ttatagtaat tctgtagcag tggacgtttg gattgatgga tgcatgtagc
    41281 accatacaca agggtttcta caggaatatc gagtttttct tccatcgcct tcatctcttc
    41341 aaaaggaact tcacgagcaa gtactgctcc aactgcgcct tttttcgccc agaaattgat
    41401 ttgacgtgcg ctcgttacta atgtttcagc atcataaata tacggtatac gcaattcagg
    41461 attttttttc atcgtataaa taatacctgt atcaccaact gtgatcacat ctacgtttat
    41521 ttcttttaaa aaagccaaat attcaggtac atttttcatt ttttcaggat gaatgatccc
    41581 atttacagca atattcgctt ttttaccata cttgtgtgct aattctacca attgtcgctg
    41641 ttcttctcgt gtaaatgaag caggcagacg caatccaaat tgctcttcac cgaaatatag
    41701 tgtatctgca cctgcagcaa gcaactgttc tgcttgttca atagtttcta ctgtggttat
    41761 gagttcgatc ataattttct cctcctacaa cttcataata gtagcatagc tctaaaaact
    41821 ttccaatact tcttctattt aaagtcggat ttttgaaaat tatttcacaa aaaatcaaaa
    41881 taaagttcga tatattctgt ccaaatataa aaagagattg tgacaaaaat cttgtcacaa
    41941 cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt aactataggg
    42001 aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct tttgaagcac
    42061 ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg ctcaatcgat
    42121 cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca ggatcaaatc
    42181 cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata tcttgttctt
    42241 tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt tcatctttac
    42301 tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt cggaaagctt
    42361 tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta acatcttgga
    42421 cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt gaagtcataa
    42481 tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa tgaccatcat
    42541 ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact tcatctaaga
    42601 gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct tcatcatacc
    42661 ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc ataaattctg
    42721 acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa agtgctttgg
    42781 ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca attggacgat
    42841 caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct ttgacagcct
    42901 catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag cgttcgcttt
    42961 cttttttctc caactgctgt agagggacac cagtccactc cgatacaact gtagcaacat
    43021 cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt gcagaaactt
    43081 tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca ttttcaaagt
    43141 cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct agtttgatca
    43201 cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct gattcatcga
    43261 tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca gaaagattga
    43321 ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca tatcttgtac
    43381 gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact tgaatgcgag
    43441 caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca tctagggtcg
    43501 ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata ttcgaagcat
    43561 caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca atgaataaga
    43621 ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct tcaaattcac
    43681 cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg cgcttttctt
    43741 gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca gcaatggctg
    43801 ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt cggctaagaa
    43861 tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc ttgttttctt
    43921 ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct tgcggtgttt
    43981 gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca gcagaatcac
    44041 cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg atacctaagt
    44101 tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc aaaatatgtt
    44161 ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa gcaaaaactt
    44221 gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg ataccaccat
    44281 aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc atttcacgta
    44341 atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca tgttctgaac
    44401 taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat actgcttggg
    44461 ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa cttaaacgtt
    44521 ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg aaattcaatg
    44581 tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt atttggtctt
    44641 cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca tcaaataact
    44701 gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca attcgtatat
    44761 aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta aaacgggtat
    44821 taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt tcaatccttc
    44881 gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca attaaatccg
    44941 aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct taattataac
    45001 gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt tagaatcaac
    45061 aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DAZUMM010000014        42864 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000014 DAZUMM010000000
VERSION     DAZUMM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.014"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..1168)
                     /locus_tag="ACG8E6_000689"
     CDS             complement(65..1168)
                     /locus_tag="ACG8E6_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFS5061134.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1797..2267)
                     /locus_tag="ACG8E6_000690"
     CDS             complement(1797..2267)
                     /locus_tag="ACG8E6_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFS5061135.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            2597..4237
                     /locus_tag="ACG8E6_000691"
     CDS             2597..4237
                     /locus_tag="ACG8E6_000691"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFS5061136.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            4391..5713
                     /locus_tag="ACG8E6_000692"
     CDS             4391..5713
                     /locus_tag="ACG8E6_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFS5061137.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(5781..6215)
                     /locus_tag="ACG8E6_000693"
     CDS             complement(5781..6215)
                     /locus_tag="ACG8E6_000693"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5061138.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(6280..7116)
                     /locus_tag="ACG8E6_000694"
     CDS             complement(6280..7116)
                     /locus_tag="ACG8E6_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFS5061139.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(7858..8484)
                     /locus_tag="ACG8E6_000695"
     CDS             complement(7858..8484)
                     /locus_tag="ACG8E6_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061140.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(8779..9114)
                     /locus_tag="ACG8E6_000696"
     CDS             complement(8779..9114)
                     /locus_tag="ACG8E6_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS5061141.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(9101..9385)
                     /locus_tag="ACG8E6_000697"
     CDS             complement(9101..9385)
                     /locus_tag="ACG8E6_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS5061142.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(9441..10964)
                     /locus_tag="ACG8E6_000698"
     CDS             complement(9441..10964)
                     /locus_tag="ACG8E6_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS5061143.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(10957..12132)
                     /locus_tag="ACG8E6_000699"
     CDS             complement(10957..12132)
                     /locus_tag="ACG8E6_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS5061144.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(12136..12321)
                     /locus_tag="ACG8E6_000700"
     CDS             complement(12136..12321)
                     /locus_tag="ACG8E6_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061145.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(12287..13981)
                     /locus_tag="ACG8E6_000701"
     CDS             complement(12287..13981)
                     /locus_tag="ACG8E6_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS5061146.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(13978..14451)
                     /locus_tag="ACG8E6_000702"
     CDS             complement(13978..14451)
                     /locus_tag="ACG8E6_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFS5061147.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(14520..14675)
                     /locus_tag="ACG8E6_000703"
     CDS             complement(14520..14675)
                     /locus_tag="ACG8E6_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFS5061148.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(14809..15189)
                     /locus_tag="ACG8E6_000704"
     CDS             complement(14809..15189)
                     /locus_tag="ACG8E6_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS5061149.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(15193..15408)
                     /locus_tag="ACG8E6_000705"
     CDS             complement(15193..15408)
                     /locus_tag="ACG8E6_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061150.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(15411..15818)
                     /locus_tag="ACG8E6_000706"
     CDS             complement(15411..15818)
                     /locus_tag="ACG8E6_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFS5061151.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(16084..17559)
                     /locus_tag="ACG8E6_000707"
     CDS             complement(16084..17559)
                     /locus_tag="ACG8E6_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFS5061152.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(17549..18397)
                     /locus_tag="ACG8E6_000708"
     CDS             complement(17549..18397)
                     /locus_tag="ACG8E6_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFS5061153.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(18434..18796)
                     /locus_tag="ACG8E6_000709"
     CDS             complement(18434..18796)
                     /locus_tag="ACG8E6_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061154.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(18797..18955)
                     /locus_tag="ACG8E6_000710"
     CDS             complement(18797..18955)
                     /locus_tag="ACG8E6_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061155.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(19096..19720)
                     /locus_tag="ACG8E6_000711"
                     /pseudo
     CDS             complement(19096..19720)
                     /locus_tag="ACG8E6_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(19717..20028)
                     /locus_tag="ACG8E6_000712"
     CDS             complement(19717..20028)
                     /locus_tag="ACG8E6_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061156.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(20072..20365)
                     /locus_tag="ACG8E6_000713"
     CDS             complement(20072..20365)
                     /locus_tag="ACG8E6_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061157.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            20559..21206
                     /locus_tag="ACG8E6_000714"
     CDS             20559..21206
                     /locus_tag="ACG8E6_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5061158.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            21267..22412
                     /locus_tag="ACG8E6_000715"
     CDS             21267..22412
                     /locus_tag="ACG8E6_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5061159.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(22493..22798)
                     /locus_tag="ACG8E6_000716"
     CDS             complement(22493..22798)
                     /locus_tag="ACG8E6_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFS5061160.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(22823..23254)
                     /gene="ruvX"
                     /locus_tag="ACG8E6_000717"
     CDS             complement(22823..23254)
                     /gene="ruvX"
                     /locus_tag="ACG8E6_000717"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFS5061161.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(23251..23517)
                     /locus_tag="ACG8E6_000718"
     CDS             complement(23251..23517)
                     /locus_tag="ACG8E6_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFS5061162.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(23772..24497)
                     /locus_tag="ACG8E6_000719"
     CDS             complement(23772..24497)
                     /locus_tag="ACG8E6_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS5061163.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(24858..24928)
                     /locus_tag="ACG8E6_000720"
     tRNA            complement(24858..24928)
                     /locus_tag="ACG8E6_000720"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24894..24896),aa:Gly,seq:ccc)
     gene            25045..25797
                     /locus_tag="ACG8E6_000721"
     CDS             25045..25797
                     /locus_tag="ACG8E6_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS5061164.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            25813..26292
                     /locus_tag="ACG8E6_000722"
     CDS             25813..26292
                     /locus_tag="ACG8E6_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS5061165.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(26543..28123)
                     /locus_tag="ACG8E6_000723"
     CDS             complement(26543..28123)
                     /locus_tag="ACG8E6_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFS5061166.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(28116..28859)
                     /locus_tag="ACG8E6_000724"
     CDS             complement(28116..28859)
                     /locus_tag="ACG8E6_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5061167.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(29035..29811)
                     /locus_tag="ACG8E6_000725"
     CDS             complement(29035..29811)
                     /locus_tag="ACG8E6_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS5061168.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(30059..30895)
                     /locus_tag="ACG8E6_000726"
     CDS             complement(30059..30895)
                     /locus_tag="ACG8E6_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFS5061169.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(30906..31835)
                     /locus_tag="ACG8E6_000727"
     CDS             complement(30906..31835)
                     /locus_tag="ACG8E6_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFS5061170.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            32027..32380
                     /locus_tag="ACG8E6_000728"
     CDS             32027..32380
                     /locus_tag="ACG8E6_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS5061171.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(32454..33110)
                     /locus_tag="ACG8E6_000729"
     CDS             complement(32454..33110)
                     /locus_tag="ACG8E6_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS5061172.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            33336..35270
                     /locus_tag="ACG8E6_000730"
     CDS             33336..35270
                     /locus_tag="ACG8E6_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5061173.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(35356..36579)
                     /locus_tag="ACG8E6_000731"
     CDS             complement(35356..36579)
                     /locus_tag="ACG8E6_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5061174.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(36573..38444)
                     /locus_tag="ACG8E6_000732"
     CDS             complement(36573..38444)
                     /locus_tag="ACG8E6_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFS5061175.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(38465..39583)
                     /locus_tag="ACG8E6_000733"
     CDS             complement(38465..39583)
                     /locus_tag="ACG8E6_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5061176.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(39666..40499)
                     /locus_tag="ACG8E6_000734"
     CDS             complement(39666..40499)
                     /locus_tag="ACG8E6_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5061177.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(40492..41409)
                     /locus_tag="ACG8E6_000735"
     CDS             complement(40492..41409)
                     /locus_tag="ACG8E6_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5061178.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(41432..41611)
                     /locus_tag="ACG8E6_000736"
     CDS             complement(41432..41611)
                     /locus_tag="ACG8E6_000736"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061179.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(41636..42760)
                     /locus_tag="ACG8E6_000737"
     CDS             complement(41636..42760)
                     /locus_tag="ACG8E6_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5061180.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT        12847 a         8664 c         7130 g        14223 t
ORIGIN      
        1 tgaactataa aaataggagt acggcatcat ctgccgcact cctatttttt tcgtcattta
       61 tttcttaaat aaaacgattc tcacgcaatt ttattttttt gatatcttct actgtttgtg
      121 ttccagctaa ttgcatgacc atttccaatt ctgttttaaa gaaatcaaac acttgatgga
      181 ctcctgtact tcctcctaaa gacaatccat aaataacggg tctgccaata gcaacaagat
      241 cggcacctga tgcgattgct ttgaaaacat gttgtccacg tcgtacaccg ctgtcaaaca
      301 cgattggtac acgtttatct actgcttctg ccacatattg caacgaatca aacgcagcag
      361 gccccccatc taattgacgg ccaccatggt tagataccca aattccacca gcaccagatt
      421 ctaatgatcg atagacatct tcttctgatt gaacaccttt cacgtagact ggcaaatcag
      481 aatgagcagc gataaattct acatcttttg gactaagttt ttgtttggaa gatttatata
      541 cagcatccat cgtttggcct actcctgatt gataagcctg aacgatcggc attggcagcg
      601 ggaaggtaaa cccgtttctg cggtctgttt cacggtttcc gccaactgtt gcatccgctg
      661 tcaatacgat tgcttttgct ccattacgct tggccacttc aagaatatcc aaattgattc
      721 cattatcttt gctcatataa aattgaaacc attgcggtgc tttctccccg cctgcttctc
      781 ttatctcttc taaagtacaa gaagcgtaag aacttgctgt atagatcgtc ccaaaatctg
      841 ctacaccttt tgcagaagct ttctccgcct tcacatgagc taatccatga gcagcaactg
      901 gtgccatgat gattggtgca gtcagcattt cttcgtcaaa atgtgtagtt gtatcgggta
      961 attctacatc tcttaataca tgaggaataa tcaaacgatg attgaatgcc cgttcatttt
     1021 cttgataagt aaataagtcg ccagcaccac tgctgatgta accataacca ccagctggaa
     1081 tcacttgtga agccgcgatt tctaaatctt ccatattgat aaaatctact attccttcat
     1141 ttgtacctgc ttgatatgtt ttttccatct caatgccttc tctctataaa atttgaatga
     1201 tgaacaatat gatagcgttt acacttttta tattactctt cgacattgat acaggcaaat
     1261 aatctgttta catttcagcc attccagatg tcactactct ggaagaaatg attgtaataa
     1321 aaatagataa aaaaccgatg ggactttgac aaaagtagtg tcaaagtccc tttttttgaa
     1381 taaacggtgt tcaaaagcca ttttctcggt attaagtcaa atttatgcaa aaatgtggaa
     1441 agccattttc acaaaatttt tcttactact cgaaactaag cgactttttc acaacctctc
     1501 gataaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaatgg caaaatatga
     1561 ggaactattt tgccatttgt ctctctaata ctcaggtcag aacgttgact tcacagtctc
     1621 ttccttaaac ggtgttcaaa aacttgcatc tgcggtaata agcccaacca aacgaaaaat
     1681 aggaggagct atttttgttt ggctgttctt attacttgat gcagggcagg tttttcacga
     1741 cctctgattc acggtgtttc tttttcatca acatttttac gaaatactgt ggaagactat
     1801 tgaaataagt gtggattgtt tctttgtgtt tcaatcgttt cttgccattc ttcatttaat
     1861 tttggattac cgttttcttc cttgaactca taaggtccta attcaaaaat cccagacagt
     1921 atttgattca cgatttcata tagttcagtg tgatcagaca catgattctt gaagatcatc
     1981 tcattatcac cacgtttgaa cctcacattc gggctctcat gatgaacctc tatgcatttt
     2041 tctccttttt ttaactgacg gacttctgaa aacatctcat aaatatcttc tccagtaaaa
     2101 ccttcagcaa gcgccagact gtccacaaga ccgcctactg tcagttcaga gattattgct
     2161 tgattggaat ctaattttaa tgtatagctt gtcgcttcta ctacgccaca tgcattgcaa
     2221 ccgccttctg atagttcgct ttttctaatc aattgaactt ctttcatttt ctcttcctcc
     2281 tcttaatttt ctacttatgt atttttatca cctgacatag gtgactccta tcaacgctat
     2341 catgtgcttt tttcctcaga aatcctattt atccattcca tctatccctt attcttctac
     2401 ttttctttct ctagcatata agagatacgc atagtattca agcaaggatt gtcgatataa
     2461 aaagaacaaa tactgctatt ttgatttctt ttggctatat tacatataaa taaaatttat
     2521 catacctgct ctttttgact atatcatcat ctgacttcta tacttcgttt gtattaaaac
     2581 ttaggaggaa tcattcatgc ttacagggtt aaacttatta aataatcctt ttctaaataa
     2641 aggaacagct tttacaaaag aagaacgggc aaaatatgga atcactggta tgcttccaag
     2701 tactgtccaa acactggaac aacaatctgt tcaagcttat ggtcaatact taagcaaaca
     2761 aacagatttg gaaaaacgta tcttcttaat gaatctattc aatacgaatc ggacattgtt
     2821 ttataaattg atgggcgaac atttagttga attcatgcct gtcgtttatg atcctgtcgt
     2881 tgcggattca atcgaacaat acaacgaaat tttcttgaaa ccgcaagatg ctgcatttct
     2941 ttcaatcgac gaaccagaaa acatcaaagc aagtttgaag aacgcggcag atggacgaga
     3001 cattcgtttg attgttgtga ctgatgcaga aggaatctta ggtatgggcg actggggtgt
     3061 gaacggtgtt gacatcgcta tcggcaaatt gatggtctat actgctgctg ctggtattaa
     3121 tccagcacaa gttttgcctg tatcgatcga cgctggtact aataatgaag aattattgaa
     3181 taatccctta tacttaggca atcgtcatgc tcgtgtagaa ggcgaaactt actatgaatt
     3241 catcgatcaa ttcgttcaat cagctaccga attattccca gaattgttat tgcattggga
     3301 agattttgga cgaggaaatg ctgcagcgat cttagaaaaa tacgaagatc aaatcactac
     3361 tttcaacgac gatatccaag gtacaggaat tgttgtccta gcaggtgtat taggcggatt
     3421 gaatatatcc ggtgaacaat tgaaagatca aataattttg acatttggtg ctggtactgc
     3481 tggtgtgggt atcgcaaaca tccttttaga tgagatgatc cgtcaaggtg tacctgaaga
     3541 agaagcacgc caacatttct atcaagtaga taaacaaggt ttattgtttg aagatacaga
     3601 aggattgact cctggtcaaa tcccattcgc tcgcaaacgc tcagatttca ctaatgctga
     3661 tgaattgaca aacttagaag cagttgtaaa agcgatccat ccgacaatca tgattggtac
     3721 ttctacccaa cctggagcat tcactgaaga aatcgtgaaa gaaatggctg ctcatacacc
     3781 acgtccgatc atcatgcctt tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga
     3841 tttaatagaa tggacggacg gaaaagcatt agttggaaca ggtatccctg ctgctgatgt
     3901 tgaatataat ggtgtaactt accaaatcgg tcaagcaaat aatgctttga tgtacccagg
     3961 tttaggactt ggtttgattg cttcaacagc tacacgagta aatgctgaaa tcatttctca
     4021 agctagtcga gcattaggcg gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc
     4081 tccagtagca aaaatcactg aattttctca aacgattgct gaaactgtcg caaaatcagt
     4141 tgtcgcacaa aacttgaatc gtgaagaaat cacggatatc aaagaagcag ttgaatctgc
     4201 taaatgggtt cctgaatata aatctttaga agattaagat cgttatagta aaagaacata
     4261 cagaaacttt gtatgacgtc taaaaagaaa aaagcgactg cggcaaaaca gcggccagcc
     4321 aactcctgct cttttgtcgc ttttctttgg cttttttcat atgatacaga ataagaaagg
     4381 atctgtaaaa atggcacaaa aaacaacaac tgcaactgca gttgaggaaa agaaaaaaga
     4441 aggtttttgg tccacaaaaa tcaccggcgt aagtttgcca ctttatttga tcatggtcct
     4501 tgtcctgatc attgcaatgg ctttagggaa attaccgacg aatatgattg gaccaatcgc
     4561 agttttggtt attttcggta atttatttca ttttatcggg acaaaaatcc ctatcgtcaa
     4621 aagttatttg ggtggtggtt ccgtttttgc gatcttcgct tctgctgcga ttgctacatt
     4681 tgggatctta cctgaatatg tagtgaaatc cacagaaaac tttgtcagca atatgggctt
     4741 ttgggacttt tatattgctg ccttgattac tggtagcatc cttggtatga accgtaatct
     4801 attgatgaaa gcttctgttc gttttattcc cgttgcactt atttcaatgg tcgtctcctt
     4861 ttttgccgta ggactcgtgg gcatgcttat cggaaatggt tttgcaaatt ccgttttata
     4921 catctcatta cctgcgatgg ctggtggtgt tggagccgga gccgttcctt tatcaacgat
     4981 ttatgcaaat gttttaggta cggatgcttc ttctatcatt tcccgcctga ttcctgcgac
     5041 agcaatgaca aatgtcttag ctatcatcgg tgcagcatta gttgctcgtg ctggacaatc
     5101 aatgccaaaa ttcaacggca atggtaaatt aatggaaaaa ggcgatcttg gcgatggaaa
     5161 acctcgaact gtgaaacctt ccattccaca gttaggtgtt ggtttgatgg tatcattgac
     5221 tttcttcatc ttaggacaaa ttggtaacta ttttgttcct aaagtccatg cttacgcatt
     5281 tatgattatt atcgttgtca tctgtaaaat cgccaaccta ttaccagagt attatgaaga
     5341 tgcagctatc atgttcaata acttgattgt caaaaacttg acagcagctg ttcttgccgg
     5401 tatcggtatt gcgttgctga atctgaatgt attagcttct gctttgacat ggcaatttgt
     5461 tgtcttatgt ctgacaagtg ttatcgcgat ttcagttgtt tcaggatttg tcggtcgttt
     5521 gtttggttta tatccaattg aatcaactat tactgctggc ttatgcaaca actcgatggg
     5581 cggaacagga aatgtagccg tcttatctgc agctaatcgg atggaactta ttgcttttgc
     5641 tcaaatgggc aatcgacttg gtggtgccat cgttttgatc atctctggtt tcttgatgca
     5701 attactttct tagtcaaaaa aacaaaaata cctgatcatc aagtatcggc ttggtgatcg
     5761 ggtatttttt tgataaagat ttacaggatt ttcaaaaatc ttttttgcat tttatcggaa
     5821 tgataaccga tgtgttcata aaattggtgt gcttctactc tgtattctgc agaattaagc
     5881 cttattgtcg ttattcctcg ttttttcgct tcttcttcta cgccagtcat caaagccttt
     5941 ccgatgcctc ttttttcagc atatttagac acagcaagtg ccataatatt gaacattgga
     6001 tcgctaaaaa tcgattcata ggactccgct tgtacatagc cagctgcttt tttagaagta
     6061 ggatcttcat aaaccaagaa aaaatttttc tctggctcct tcagcagttt cttcatttgt
     6121 ctcaccgtaa cggttaatgg tatatcatac cctaattgat ccgcattgat ttcacgtagt
     6181 gcttctgcat cttctaaacg catttcccgt aacatccaaa tcaccagcca atttctgatt
     6241 ttttaaaatg aattttttta tcatagtata ctcttttttt catttagaac aatatgtttc
     6301 agtgattttg acttgcgtta gtctagattc tcctaatgtt tgtaagatga caaaggcatt
     6361 gtaacaattg accaaaagaa ccacgaggac aattacttga aaaataaagg tcactttttt
     6421 tggagacaaa accctgctaa atcttgctcc ccacaaacct ccaataacag cagcggggat
     6481 aacataaaac aacatagata aatcaaagcg cataaagcta cttgttaaag caatcgttac
     6541 caattttgcc agttgagaaa agaaaatcgt agatagagaa tatagtgttg cttcttttat
     6601 tggcaatgca aacatcagca tcaacaaaga aacattgact ggtccaccac caatacctaa
     6661 aaaactagct aaaaagccca gaaccccccc acatataagg taccaagtcc atgacgtcaa
     6721 atgaaatttt ggctgatgat gcttggtgta gaagaacgca aaaaccaagg tcaataccgt
     6781 caaaaaaatt tggatcagct gcacatgctt ctcgttttca aataactgta aaaaaacttc
     6841 gaatacaatg ttgccgacta ctcctccaac aacagctcca ctagataccc aaccgacaat
     6901 ctgccaattc agtgatttcc cgcttgatac ttgtcgcaca gtcgaaacaa tggacatcgt
     6961 gaataccgca actgttgagt aaaaagaaat ccctgcaaca gaatgggcgc cgataagatc
     7021 aagaatcggt ttgatcaaca cgcctccacc catcccggaa atagcgccaa ctgtgttcgc
     7081 cagaacgatc accacaaaat aaatcagacc aatcatcttg tatttccttt ctcttgttta
     7141 tatcttttag cgattgttac tcttttcttt tattatacag taaaagaaac aaacgaggct
     7201 tcgaaagctt ttctactttt taaggaaatt tagtgctaaa cgaaaagagg ttgtgaaaaa
     7261 gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agctcctcat aattttcgtt
     7321 tggttgaact tattaccgca gatgcaagct tttgaacacc gtttaagaaa gaggtcgtga
     7381 gcctgccgtt cagctccgag tcacaaggaa caatttcaaa aaacagcttc tcatgtttgt
     7441 tgaatattgt gacttgtgac cgaggagttg gcaggagaac accgtttaag gaagagactg
     7501 tgaagtcaac gttttgacct gattattaga gaaacaaatg gcaaaatagt tcttcatatt
     7561 tcgccgtttt tcgagcgaat gccgaggggc agctgattga acatcgttta tttggaaaaa
     7621 gggattgtga caagacgttt gtcacaatcc ctttttttat atcttattaa ttcattcttt
     7681 tgttctattc ttcttcttcc gccatgaatg agaaacccat atcaaagaac tataaaggtt
     7741 atcaaaacag cgtttatgac taaatgaatt agagttatct atcactattt ttcaagcgat
     7801 ttaaaccaaa tggcatacaa tatggcatac aaaaacttca ataagccgtg ggggttttta
     7861 aatttcttct tgttctttaa aatcaatgag tggagctaca taatttgaat ccactttttc
     7921 aaacgtcact ttttgcatcg acgaatgttt tctttcccaa gataacgtaa atagatttga
     7981 ctctggactt ataaagtcta cttcaccttc tagtattact ttcttatgca aatattcctc
     8041 taataaatat agacttgcta aagcatacaa cacattttcc aaatttgctt ccttataatt
     8101 tgaagaacga ccatgtttta ctctgttata tattttccac caatctggtg tatcttcagg
     8161 ccacccatct ccaaaaggtt ttagtgaagt caatgttaaa gaattcataa ccctaacacc
     8221 attatccaaa cacattaatt cgctagattt aataacagct ttaaaatcag ttatgctttt
     8281 attgctactg ctatcaaatc cgcaaagttc tttgaaaata atctcacact ccgaaccaat
     8341 cgctagtagt agtttttgat attcaataga gaatgttttg aaattattct catcgagcat
     8401 cacaaaatct gtggtattaa taaactcatt ttctaaatgt agataatact tccaataaaa
     8461 atttgtaaac tctgctttgt ccattaaatc acctcttttg aattataaca aaaaaaacac
     8521 gtccacaaca gacgtgttct ttgatttcta cacaatcagc ttgccaaaac catccaatca
     8581 gtcctagcat gcctgtatta aaattgaacg ttcatcatct atcatctttc ttagtccagc
     8641 atgtttgcaa ccacacctac aattttcagt ccaacaatag atttcgacta gctaagtctt
     8701 gcaaaattca ggcagctaac ccttcattta ctaccttaat tatactaaat acccacaacg
     8761 tttacaagtg taatcattct aagaaatctg tttagctatg atcgttgtat aatctttaaa
     8821 cgccgttcct tcattgattg aaataatttt ataactcttg tttttccaag ctactttcat
     8881 atcgtttgtt agttcataat cttgatcgta tcgaataata aacgtcaacg ttccttcaag
     8941 aatggtacct acactagcaa gcacgtcttt tagcatttgt gaacgcacat tagcccaaca
     9001 ttcaaataca gtttcttcac tatccaccat ctgcccatct tcatctttta cccgttttgt
     9061 ctttttaaac ttgactctct cagtcaattc atttacattc ttaatgattg gcataataag
     9121 cacctctcat ttgttgtact aatgcttgta ccgttactgg tatccgttcg ctgctggctt
     9181 cttgacggtt caaataccaa tgttgagtaa gtaatgacac cgccaaatca aattgctgat
     9241 aattatccat atctttatcc gttgcgctac tatcaatagc actttttata tattcgcctg
     9301 ctgtatcaat caattgttga ataaagtcat catcaatagt gtgatcgatc cgcatacttt
     9361 tttttatgct atccaaaatt accattttat ctctcctaat gaaaaaaagg cagtgggata
     9421 aaccgccgcc caatattttt ctatactttt ggtgttacaa ctggcgtaaa ctcaatatac
     9481 actgaagcat tttgatcaat tttcttataa tcgttgcgaa cgatcactgc aagtccttgt
     9541 gagtagtaat cgaatttttc ccattgagtc gttacttgat ttctacgagc aacaaagata
     9601 gattgtgcaa tatcccccat aatcataggg aatgttccag ctttaggatt ggcaaacaat
     9661 gtatctgcaa tcaatactac tggcataccg aataatgatt taccactagg tgctgtcaca
     9721 tcgggttgta aaatgtaacg tccatctgaa tctttcaatg tatctaggtg gtaatatccg
     9781 ctttggttta gaattaccat tttatttaat gctgggtcta atgccacatt gtatagttgt
     9841 ttcaaatcgt ccaacgtagc agccgttttc ttagtgaatg tttttaataa atccatgata
     9901 tgcttattgt cagtgttttc taccaatttc gctaactgat ctttgacctc ttgtacaata
     9961 ttcactgctg aatcttccac aacctcattt gataaggcaa tcttaccagc acgagtttct
    10021 actttataat caactgaagt aaacatttca gcgtcaatat caccaatttc agcaagttca
    10081 gcttttgttg ctaacactgc ttgttggtta gttgctactg gatatttacc ttgtccattt
    10141 gatactgttt ttactgttgc atattgagct aagttataat ttgaacgttt caaatcaaag
    10201 acttcaccga ttacttcttc gggaacaact gccgctgcat ttacagtagt gactccatca
    10261 cgcacttcgc cttgtgaacg gatatattct tcatacccac gcaattctgt tttttcgtta
    10321 tcgattaaag ttttttccat gttattactc tccttttgtg ctttagaagt ggattctaag
    10381 ctattgtttg tttgttttgc ttggttgtta ctcataaacg attcgtaaga acgcttattt
    10441 acttggacat ttgatgaatc ataagcggga acggtcacga cactaatttc attaagtgct
    10501 ttgattttat ctattgatcg ttcaattgtg ccatcttctt ttttgtcgaa tgaatctaat
    10561 ccaagcacga acccaaagga catagaatca acaacccctt ttgagatatt ctcatacaca
    10621 tcatttgaat aggtcgtgtc ggttagctct gcttcaaaat gtaatcctgt atcatcgaca
    10681 ttcaatttca acgtacctgc tttgacacta gctagtggct ttgaataatc gtgtccatat
    10741 aataagaaca catttgataa atccacctct tttaatgctt cgggtgtaat cacttctaca
    10801 aagccgccta aatcttttga cggttgcccg aatttcaacg catagcctga aatagttttg
    10861 ccgtctgttg gttgtttttc attttctttc tctactttgg gagaatcggc tgttagctca
    10921 gcttcttctg tcagccgttt ttccttttct tcttcattat tcaactggtg taactccttc
    10981 ctgttctttt aattgatatt tctccaacgt atctaaatac gtaaagttca aacttgctag
    11041 taaacgatcg ccaccatcca tggggggtag ccccattttc gaccgcccct cattaatcgt
    11101 gagaagtgac cctttgacct gatccaatac attttttact tttgtttcag gatcagtctc
    11161 tagtaactga tcggaattaa agcggatatt ggtttcaagt ttcgtatcta attcactcac
    11221 aaacacatta aagtaatgaa tcagcgtact ttggatatac tggagattac tttgaacggt
    11281 gctagaatgt tcattttcaa cgcctaaacg ctcaatcggt acaccaaacg ccttggcaat
    11341 ttgttttgtg ttccaatcat tggaattgat aagcttcaaa acatcggtgt ttatttctaa
    11401 tgttttgtag tccattgttt catcaagaat gatcgttcta agagcattat ctccactact
    11461 tgaaccgttg gcttcttcaa acttttcacg aattgcgttc tttgctgatc cgtctaaatc
    11521 tgacttatga accttcaaaa tacctgaccc actgacacca cgagtaaaga aattgtgcaa
    11581 tgtgcggttg ccagcttgtt gaattttcag ttcatcacgt aacgcataaa gtggtggtaa
    11641 tcctgttaaa ccatcttggg tgaaatattt gaagtgcaaa atatcgctag acttcacacg
    11701 tcttttttta tcaccaatct gataagacaa cgtaccatta tctaattgag taaccgttac
    11761 acttgagtta ggtaataagt gaatttcttc taccttttca ccgttgcgtt taatctctgc
    11821 aaagctatta ccgtttagca acatattgac cgccaaagca aactttaaat gccaaccatc
    11881 catttctgaa ttaggtttct cgtttagtaa cttcaccagt tcattatcag cttgtggcat
    11941 attgtttttg accaattgaa tcggacttga agcaatatca ctagcaataa ttcgaacggc
    12001 tgcaaacaca tcactatttc ttaatgcacg aacgcttgta aagctggtgg tgtaatcatc
    12061 cgattggatt gataccacat gttgtaggaa aggatctccc gttgtatttt gtctcgcttt
    12121 aaaaaatgcc atcttttaac ctcccttctg ttctgtattt agcatgaccg ctaccccaat
    12181 aagcactatg cccagcgcta acaagccata gtagacattt gttagtaagg tgatagcaac
    12241 caacacacac accaatccaa gaagtaaaag aacggtatga atgtaattag aaagaaaatt
    12301 cttcacttgt ataaaattca ttgtctgctt tcgctccttc cgttgtggtg aagtaatcca
    12361 ttgcaaacac ataggcattg attaatgcag ctattggatc aattttgtta ctgttctttg
    12421 atttattaat ctgaatgccg ttgttatccg tttttaaaat agcgttgttc actgcatggg
    12481 tgagaatcgt atttttattg tgaataacgt tgccttcata aacttgttct ctaaaagttc
    12541 gggtcggaac gtttagagta atcgttcctt gtcttacttc taacattggg tagttggctt
    12601 tttcagcttt actgataagc gaattagcgt tatacgggtc ataacaaatc cccatacatt
    12661 ccaaatcatt ttcttgaatc aaatcttgaa taaactgaaa tacttggtca tagtccacga
    12721 ttccactttc taattgagta atggaacact caccagcttt ttctagttct cgataaggca
    12781 aaccgtcgcg cttttctttg tcttgaagcc catatttggt ggctacaaag ctatgtgaat
    12841 cacaataaag ttcgccgtta tctagcggta cgatccatga aacgctagtt aaatcatctg
    12901 ttttagataa atccactccg atataaacgg gcttgcctgt aatatcttgt ggttctactt
    12961 cggttgcctg ccagtcttca gcaatcatat agctgtcctc actcgcttgt ctccataaat
    13021 tgaagttttt caccaataca gaattaaggt tattttgttt caaagcaagg ttcacatcat
    13081 cttgaatggt tggaatcatt acttttttga tttcttcact ttcaaaaatc ggattggctt
    13141 tgatccattt ctcttgatcg tgaatttctt cgggatcgtc caattcccat atcgctataa
    13201 agtacctgtc ggcttgttct tcgcctttta gcacacgatc taacaataag tattcctcgt
    13261 acataggaac gtttaaatca agcccagcgg tactaataac acacaataag ccgttctttt
    13321 gttgggtcat tcctgactta atgacgttgt acgttttacg agtttttgct tcgtgccact
    13381 cgtcaagaat tgcggttgtt ggtgcaaacc catctagcgt gctggtttca ctagccagtg
    13441 ccatagcgaa cgaattagag ggcaagtgct gaatcttaga gttcataatt ttcagttgtg
    13501 gtcttaaaaa cttactagac ttgaccacgt ttctaagtga attagacagc atatcatagc
    13561 ccaatttagc ttgtttcaaa gcgttactta caaacaatac ttgccttgct tctgctggtt
    13621 cttgttccat aatcaacgca ttggcagcca tgccacttgc gatatacgtt ttcccattct
    13681 tacggctcat actggtaaac gctcgattga atcgcctgta attcccttct ttagtacgcc
    13741 agccataaag actgcctaga atgaattttt gaaatcctaa catcttgatt gctttaccgt
    13801 ccgtagatgg tagcaactca atgaatttaa tcgctttggt ggcttgttct tcatcgaaca
    13861 cataagggaa gctatctgac ttagaacgct ccaaatcgct tatatgacgc tcacacgctt
    13921 ggataatttt cttgggtgca atcagcttac ctgataggac tttctcaatg taatcattca
    13981 tgaattcatc aactctttga acgggtcttc gggttcatca tttttaacct tgttcaaagc
    14041 tagttttgat cgtgattcta aggtcatacc tagtgaggtg gcttgttttt tcaaatcact
    14101 catagcttgt gattgaaccc gaaccgctgg attggctttc tttactccgt tttcgcatgt
    14161 gataaacgta cctgattttc ttatctcggc ttcggctgtt ttcattcgag caaaggctaa
    14221 acaataggct accaatgaac cataatctgc ttcactcaat ggataatctt tttttaatgt
    14281 tggtacgata cgattccatt cacttctagc gctcgctgcc atccagtcag ggggcgtgat
    14341 atttattaat ggttgctgct tgaacaattc ttctctagcg tccacacgga ttgctttttc
    14401 ttcacttgat atattgcctt ttgtatcgtg taatatttta gggtgtccca tgttgtcctc
    14461 ctttcagaca aaagaaaaag gaacgtaagc aattaagctc acattccttc gtttttcgat
    14521 cagaattttt ctttatctaa tttatctata taaatgattt ttccgttctg catttgaatg
    14581 gtcacttgac caaaatgagg taactcaatg gtgcgctgtt tccctgtttt atcagtaata
    14641 acaattgtat ttttcaataa atctcctact ttcattatat actttttcac ttgtgattac
    14701 aagtgtaatc gttatttgta attaccctta tgccttttca caacggttat ttgtaccaaa
    14761 atgagtaggc atcgattgtc agtaagtttc acactatgcc cccttaactc aaagggtact
    14821 cttttctcgt ctttgtcttt cattggctgt cttggaatta tggtgtgcat ggcataaaga
    14881 acgaaaatta gtttcatcta accgtctatt ccaatcatct tttagttcaa ttttgtgatc
    14941 tacaacatca gctttcctta tcaagccttc ttctaggcaa tcctcacata ctggatgatt
    15001 taaccgatac aagtatgaag cctttcgcca tgttctactt tgatagaatt gaaaatattt
    15061 accttcgctt tgctttcttt cttcacgctt aattgccgtt tgctttgctt tatgcttccc
    15121 acaatacttc tgcctataat caatcaatgt tttacatcca gcatgattac attgtttttt
    15181 aactgccata gattaccaac caaagcgatc tcgttcgtca gaatcatctt gttcccattt
    15241 tgatttgtat tcaatataag gcgaaactat cttcaagcac gttccgtctt taaaattaac
    15301 tttagtgatt ttatcttcct caaaactaat aaagtctaca tcttcttgag tgtttgaata
    15361 ctcaatgtgt ttctcaccat cgaagtatct aatactttcc atatgcataa ttattctacc
    15421 tccaataaat tagttatcaa ttcatcatct gttagtagca aagccaagaa cttatcatgg
    15481 atgtttaaaa acaaattatc ctcattcgat gtaagctctc gttgtctcgc tcgatcatat
    15541 aggttaagca gacaattaaa ttcatccatt agctcacctg tatcatcata gttgccacta
    15601 tctaccaatg atcccactac tagcgattga caccacgcta ctgactcctg cagccaatca
    15661 ggttcttctt ccattatctt taatacttct ggatcaaatg tggaataatc ttctgttacc
    15721 aatcccacac taaatgccag cgtgttcatt agaaaccgat ccatttcatc tgtatattga
    15781 aagttcggtg ctttgtttgt tgaaataata ttgagcattt actttcctcc ttgattcgta
    15841 acacgtggta acacctacgt aacaggtacc cgttaccact caatcccaac ggggacaagg
    15901 gttctagcgt tttggtaaca ggtaacgggt aaaatccagt aacattattc cccagcatat
    15961 tagctaacta tttcaaaagt tttaaggaaa taaggtgtta ccccgttacc aaattgctgt
    16021 atcccttggg gcagtaaggg taaactagta acgggctagg tgttactgac ccgttactac
    16081 ccgttacttt ttgtactttc cagctcgatt tttattttca taggcatttg acttattgta
    16141 aaatcctcgt cgtttatttc tttttggcag ttgcttccgt tcccaaccag gaatactgct
    16201 aataatcaag gcaatctttt tagtttctgc atctgatcga ccattcaata aatcctttgc
    16261 ttgtttatca aaaacagctt ccaaaatatc agcggtgagt acgctatcaa ctaaataaaa
    16321 ctcttgttgc atagtagctc cattatagaa atgctgtaaa tttttctcat tacatttact
    16381 gtcaggatag ttctgataat aagaacgtct cgcccacgct ggagcttctt cccatttata
    16441 cgggatttcc atttccaaat atttagtgat tgcttcttta attggattct ccatcttggc
    16501 atcctcttga tagtcttttg caatttctaa gacttcttta tcttcatcgg gactaaaata
    16561 gattcgttgt cctttatcaa aaagcacctt tgcttcagct aacacttgaa gaaaatattc
    16621 atcctttgtt ttaaacggat tcatctttgg cttattctta caaggcaaag gataaaaacg
    16681 gcggtttcct gtatcatctt tcaaatactg cccatcattc gacgttccaa taaatacaca
    16741 ttgccttgcc caagctatga cattccgacc ataaggaggt ctaattttat cgatcttggc
    16801 actgatgaag ttttttatct gttcaatttt agttttgttc attgaagata attctccaag
    16861 ttcaataatt acattgcctt gaagctgcat ataatcatct ttcttttcac ctagcgaatc
    16921 taatgaatcg gtgaaaaaat catccgtgta aagtaatgct ggaacggtac tcttaccaat
    16981 cccttgcggt ccatccaaaa ctggaacgat ctcaaacttt attcctggtt tatagatacg
    17041 tgcgactgat ccgactatcc aacgttttgt tacttctcgt gtgtaaaggt tgtcgtctac
    17101 ccctaaaagg tcaataaaca gtgtttccag tctaggctgt tgatcccatt taactttttc
    17161 gatacgttct tttactgggt tgaaaacatt ctttctagcc acatttacta ctgctttgtg
    17221 tattttgttg tcatcaaaaa ctacattgta gttttcttca atatatgccg ttaaggcatc
    17281 ctcaaaactg tcttcccatt ctccagcggt acgattgatc caaggagaat cttttaatag
    17341 gtgaatgtat ccgctaaatt cgttataagc aattgttcca gctaaattgc tatcattggt
    17401 taaaatcaat tctgcattca caacactgtt acttttaatc tgatcgtctt tattgcatct
    17461 taatcgtgac atccaggttt tcaattcaat gatattgtct tcacgctcgc tggcttcggg
    17521 aaagtctaaa atactatcta atttatgctt atgagccatg agctttcccc ctcaaatctt
    17581 ttttaatcat cgatcgaata atttgattca cttctttatc ttttagcggt ggttcattgt
    17641 acgcatttgc tgtataaatc aattctgcta aagtttgatt atctacaccg tgccatagta
    17701 accaaccgca taacttagtt aaatgatcgt ttctactacc ttcgccagcg ccttgaacaa
    17761 tcatatctaa tttttttgct gtccctgttt ttgctacggg tcggtattga gtctttattt
    17821 cttgcggttt ttttagcagt aggttcacta gccaatcggg agctggtgca atattattta
    17881 gagatacgtt ttcaataggt tcatatgctc tcccaacact tcttgaaata atcatagttg
    17941 gcgctattgg aactccttta gtgataatat caatcccgct taaactttta cctttatcat
    18001 cacgcccgaa gatatcagca aatgcacctg tttcttgttg gataattaca ccatcaggaa
    18061 ttttaaagaa gaaatggatt cctttacctg gtgtgatttc cgtataagta gaaggaaatg
    18121 gatcatacat tgaagcaagt tttttataat tctcaacgcc gtttcgtgtt tcagaatgtt
    18181 gatctaggtc aactaccacc atttgatgat cttcaagcat taacccaata tttctagttg
    18241 catcttccgt ccaccattca ttaatttgat tagaatcgtt agttgcatca ttccaaccac
    18301 tagagccttt tattggaatt ttagttccag gagataaggg ataaattgac catcccgact
    18361 tttgtaattc cgttgctgct tgataattat ttgccaataa acctctcctc ctttttcaaa
    18421 atactggtat aaatcattca gacacctcca tgagcttcaa ctgctcaagt tcgtatttca
    18481 acccatcccg aacaaattca gcacctttta attgattctg atactttacc catgtattaa
    18541 atgttttcac ccttgcttct tgcatatcag taatgtttaa ccatgcggtt aaaatctgat
    18601 tttttagctc gttgatttct cgacaaatta ttttgtgttc tgcatgtttt ttgagtacat
    18661 acaaaactgt tagtttttca ttgaatgaat ttccaaagta ggttttcccg atattatttt
    18721 ctagttcagc gatcccttga ttaatttcct ctaattcaag gctatcgagt tggtttaata
    18781 gttttgtttt gtacatctac ttatgccctc cagttcttaa ttgatagtcg tactcgtcat
    18841 aagcagtgaa gagatataac gctaatcctg caaagatagc tatttttgca attaatggta
    18901 ataagccgaa aattccgccg attaaaatgc ttcctataat taagtttcgt ttcataatgg
    18961 ttccctccga ttttttaata tggtataatc ggaacaaaga atatcgggtt ttatcccaat
    19021 taagttgcct ttatcctgtc cgccaagact gttatggata aggctttttc tttgttctcg
    19081 atcatgttaa tgctcctaag ttgtctgcaa ttcagcagaa cgattataaa ttttctcact
    19141 cactgaaaac tgcaacccat atttttcttt aacagtcatt aattctactg tctcatctaa
    19201 aataggttca cgatcaacca acatttcagg agtcatatct ttctttttta ccattttgga
    19261 atgaccataa atagttgata cagctttatt ggctatcaca ttcgctttaa tgtagtctac
    19321 tggttttggt tcagtaatag cgtggcttaa tttagtcatt gcttcttttt ggtgttcttt
    19381 gtctagcatg cggaatactt gaaagccttc aagtcctgtg gactggcgta gttctttgat
    19441 gatgtcgaaa atccagtctt gaaatgcctc ggcttctttt ttacggcttt tgaatgccat
    19501 gcggtaaata ttttttttcg ttgatgatgt ttacatcttg tgttctgccc aatgaatcga
    19561 tgaccttact agtagtaacc ccatctttag gcaaagagtg aatggctcta gtaacctgtt
    19621 taagacctaa cgcttccgca acatctttcg ccactgccca ccattcatca ttaatatcga
    19681 caaatcttat aatgtgtccg ttccaaattt cagttttcat tcaacgtcct ccagttcaag
    19741 ccctagcaag ctcgctaaca actcaacttg ctgatacaaa atttcttgat actcttcaat
    19801 agaaagtcgt tctttaaact ctgcttgtac taaatcgttc aagccacgca atactccgct
    19861 taccgttggt tcttgtggta gcaatttttg atagtccatt tgttcgtaca aacattcaaa
    19921 cgtttcttct agcgctttgc cttccaagtt tgctaattct gccgctgtgt cttctgtaag
    19981 aattgttgat aatacttttt cgattgcttt attttgtgtc gattccatta ttatttcctc
    20041 ctattaatcc ctttgccccc acggcttgcg tttatttttg gtattcttcc ataaagcgat
    20101 cacattcatc ttgtgaaatt cttacgactc catcaactat aatcaacttt aaaccgttag
    20161 gaacatattt ctgcatcata gtattgtatg aaacgttcat atacttagca gcttgtgcct
    20221 tgttcatata ttttggaaac tggtactttt tttctaataa tgaaagtaac gttccttgag
    20281 tttgctctac ttgttgaaca actcgatcgt caataatttt aaaagctgga tcatcaagcg
    20341 tgagtttgtc aatttttaat tccatattta tcccccctcg ttagcataca tttctataca
    20401 aagattttaa aaaaatgcaa gaggctcttg aatacatttc tatacctctt ataaactcaa
    20461 tataccatca taccttttca tttgcaagaa ttttaacata cttttttgaa cttttatgtt
    20521 tcacacatat ttaaatcgtg atatagtaga gaaaatatat gaatggtggt gaaaatgtgg
    20581 ctagaaaaaa ataccccgaa gcagtggaga caggacaaag aattagactg gctagaattg
    20641 aaaagggact ctcaatggaa caacttggag gtaaattatc cccaccggcc agtaaaggtg
    20701 ccgttagtaa ttgggaaaat ggctataatc ttcccaataa caccaggctt aaacaacttt
    20761 caaaggtatt agatgtatcc actacctatc tattaaacgg atcttataca ttaagagata
    20821 tgcatatgat gcccgaaatt aagcgaaatg tgcttgaaaa gcaattgaaa aaaactattg
    20881 aaaaaaataa ctctataatt gattctaatc tttctaatgc tatcaataac atcgaagaag
    20941 ataacctgac tgtctcacag aagatttatt taacaaacgc aataaaatat cttaaaacag
    21001 gatctcgaat tgaatttcct gacgggagta ccccaattgc ttatctcgca gctcttctaa
    21061 atacgctttt acgatatgac tcattaatta atgattcaga aaaaaataca gatgagaaaa
    21121 aagagtatca agaattttat gaaaatgatc ttctaaaaga gttcacagct cttgttactg
    21181 agctaaataa acactattca aaataataat aaactgcctc ctccaaccgc ccccacggct
    21241 tgctacacta tggagggaaa actattatgg caacattcaa gcaatatgaa actaaaaaag
    21301 gtaaattttg gctctatgaa gcctatcttg gaatgaacaa aatgactggc aaacctgata
    21361 aggttcgtag acgaggttac aaaacaaaaa aagaagccca gcttgccttg tcacgcttgc
    21421 aagtggacta cgataaaaac ggtattaaaa aagcgaacaa cgaaacgttt aaagaagttt
    21481 atgacttgtg gctagaaaca tataaaacca ctgtccgaga agccaccttc atgaaaactg
    21541 aaatcaagtt tagaaagtgg attcttccaa aatatggaaa tcttcgtgtg aatgaagtca
    21601 cggtaaaaaa agcccaacaa attgtaaata gctgggctga aactactgac caatacaagg
    21661 tacttcattc aacttctaag cgtattttta gctacgctat caatcttggg attattgaga
    21721 ctaatccact agacaacgta ttaatgccta aaagaaccac cactaagcat aaagaaaagg
    21781 tcaaagttta cagtaaagaa caactgatca ccttgtttga ttacctaaac agcaaacctg
    21841 aaacttatcg aaacgacttt gataaaacgt tactacgctt tctcttttat agcggggtta
    21901 ggatcagtga agcccttgca ttgaattggt cagacattga ttttcaagaa aatacggtca
    21961 ccatcaataa aacactaagc caatccaaaa atggctataa aatcagtgat cctaaaactg
    22021 attgtagtgc tggggttctt ccactagatg aaagaacaat ttctatatta aaaaaatggc
    22081 agattaatca gcggaaatat atgctgacac ttggtgtaac tgatccgact atgatttttt
    22141 gtggtatcta taaagaacta atcacacacc acgctattta tgcaagattg attactattt
    22201 gtgaaaaagc tatggttcca tttctcggtg tgcatgtagc aagacacact catgcttcaa
    22261 tgctcttgga cgctggagct actatggtag aagttcaaac tagattacgg catacaaaaa
    22321 ttagtcagac gatcgattgt tacggtcact tggcaaaaga aacgaaagaa aagactgttg
    22381 aaaaattagt acaacactta aatattgact agatggcata caatatggca tacagtaaaa
    22441 aataaaaaaa ggagaaaccc tatcatatca aggtttctcc tagctttata gtctattctt
    22501 cttcttccgc catgaatgta ttgaatactt cttcaatcat gtcccattct gcgtctgttt
    22561 cgatttgttc taactcgcct tctgttccat cttcgttttc gatgtatgca taagcttgta
    22621 attctacatc ttcatcttct gaaacaccag caggatataa aagaacatag ttacgtccaa
    22681 attcttcttg tccgtcaact gttagtagga tttcatataa tgtttcattt ccttggtcat
    22741 ccaccaatgt gatgtgttcg tgtccttcat ggtcgtggga atggttatgt tctgtcataa
    22801 gattacctct tttcttttat tattaaaata attgcttacc tgagccgtct aaataatttt
    22861 gtaagatcat cacagctgct aatttatcga tgactttttt tcgtttggct cttgaagtat
    22921 ttgcctgctc tactagcata cgttctgcct gaacagtggt taatcgttca tcttgataat
    22981 caactggcaa ggagaatagt tctttgatct tttctccgta tgacatggaa gcttctgccc
    23041 ttggtccgat ggaattgttc atatttttag gtaatccaac tacaaaccgg tctacttcat
    23101 attgtgctac taattccttc aaccgatcaa agccaaactc ttcttctgct tcattgatcc
    23161 gaatgatctc tactccttga gctgtccagc caaaggggtc actgatagca acacctactg
    23221 tcttagaacc gacatccaag cccattactc tcataaattc actccatgat ttgagaggta
    23281 gtccttgaca attacttcca tgatctcatc gcgttcgtga cggcgaatca aatttcgagc
    23341 atcacgataa cgaggaatgt aggccgggtc tccagaaagc aagtagccga caatctgatt
    23401 gattggattg tagccttttt cttccaaagc ccgataaaca atcgctaatg tttcgctcac
    23461 ttcttttttg cgactatcgt caaaaccaaa acgtacggtt tcatctgtaa aacccatgat
    23521 tctacacctc tttcccgcaa ggtaattcca gcttaccttc tacaacctta ttttactcga
    23581 agaaagggaa aactacaaac aaatattcca aacttccgaa taaattaaga aacactattt
    23641 gtatgttttt ttcctttttt ctgtagcttt atcaaaagta ctcttttcat aaagtatacg
    23701 ctatatttct ttgaatgtat gtttttacac aattttttca taatattcat aaaaaattcg
    23761 aatagctgct actaattttt tgtttttgtt gcttgtcctg tctcataatt gatcgtgtag
    23821 ccatcttgaa cattgtagat aaagacattg aattcaattt ttttgtcttc aatgctttct
    23881 gcttccaatt gcactcccct ggctaccagt tcattcccct caaaatgagg agtgacacgg
    23941 tagcgtacat gatgatttgt ttctttgata tatgccgcaa tatcattttc gtgagcaagc
    24001 atgtatggag cattcaacga acgtgtccct gtcatcaagt ttttaatgtt gttattttga
    24061 ccagtcagct ggaaaccaat taaatggctg cgattatata accattgccc gtcacttaat
    24121 ttcttttgtt tccaacctgt cgggtcaata tacaaaggct ctctcttttc agtagggaac
    24181 aaatcttttc caagcatagc attggcttgt cctactctgt tcaatttatc aatatttgaa
    24241 tagctttccc atgttccttt ggaaaggcta agatcctctt ttgtaaattc cggctgattc
    24301 ccattgattt ctatttcttg atgttttcca tcatattctg gcacatccgc tacttgagaa
    24361 gcttgtggtg tggaatcaaa aatagttgta atctgctgaa agatatctga atctttttgt
    24421 gtggtggaaa caccgaatgc ccctgcaatc acagcagcga cgatccccac gatcgccttg
    24481 gttgtttttt tgctcatttc tttactcctt tactaacagc tccctaaatc ttaacatatg
    24541 ttcccctata aataaatact agaaagaaat agaaacagca aaaaataaaa cctgttttaa
    24601 gatggcttaa aaatacaaaa aagagacgga ttcctcgtct ctaatcagtc accactctat
    24661 ctgtaaggta tttattatta ttcaaaaatt ttcttcatac tcaagacaat acactgattt
    24721 cacaaccgct tggtaaacgg tgcgcaaaag cttgcatctg cggtaataag tccatccaag
    24781 ttaaaaacat aagaagttgt ttttgcttgg ctgttcttat tacttgttgc agagcagctt
    24841 tttcacaacc tattttaagc gggtgacggg aatcgaaccc gcgacatcag cttgggaagc
    24901 tgggatttta ccactaaact acacccgcaa tacaaaaaca gtatacgcct cttttattcc
    24961 ttcctcaagt ttttttattc tttacgcttc tggtatgttt tgtagtaaaa taaaactaat
    25021 caatcaatta ttggagaaga tgctatgacg ggaaaagaat tagatctgtt gctggacact
    25081 tgtttactcg ctgggaaaat catgatggaa agcaatgcgg agatgtatcg ggtagaagat
    25141 acgatgagtc gaattgcttt agcttctgga aatttccgtt tggtcagcta tgtcactcaa
    25201 accggtctct ttattggtct tgatcgtacc tcgaccatcc gaatggaaca aattaccaac
    25261 cgttcgatta atttagaaaa agtagtaaaa gtaaatgatc tttcacgtaa atatgttgct
    25321 tcagagctaa cattagaaga actttactgc gaactgcaag caatcgaccg agaccgttct
    25381 ttctttcctg tctggttgca aatcgttagc gcggcagtga tcagtggaac aattatggtg
    25441 ttatttggcg gaaagatgac cgatttacct gtgactttag tcattggagg attgggatac
    25501 gcagcttatc tatatagttt gaaatttttt agaatcaaat ttttatcgga atttttatct
    25561 tctttattga ttggcatagc cgctatctac agcgtacgtt ttggcttagg aagcaatcag
    25621 gatctaatca ttatcggatg tgtgatgcct ttagttcctg gagttcaaat tacgaacgct
    25681 atccgtgatt tgctagctgg acattatgtc tcaggagttt ctcgcggaac agaagcgatg
    25741 atgactgcta cgatgatcgg ttttgcgatt gcttttattt tccagctttt ttattaggaa
    25801 aggaacacaa gtatggggac gatatttatt cagttttctt ttagtttttt agcgactgcg
    25861 gcatttgccg ttattacaaa tgtacctagg cgttcattga tttgttgtgg tctttctggt
    25921 gcttttggct ggatgatcta ctggatctgt gttcaaatag gcggatctgc tgcttttggt
    25981 tcattgcttg gttctttagg agtagcattc atcagcgatc ttttctctaa acgtttgaaa
    26041 atgcccgtaa cgattttcaa tattcccggg atggttcctc tagtccctgg tggtttagct
    26101 tatcaagcgg ttcgaaatct cgttactgaa tcctatcgag aagcggctca ttatgcggtc
    26161 caagcaatta tgattgctgg agcaatcgct ttaggtcttg tattatcaga agtctttaac
    26221 cataacatcc gtaatttcaa agtaaagcgt gacgcaatca tgatgaaacg aaaaaaacgg
    26281 ctttttaaat aaacgaagtg taacaaagaa aaggttgtga aagagacgtt cagccttgag
    26341 cgatgacaaa atatctcaca aacattgcga caagtaaccg caggagcaat aagaagtaat
    26401 tatgagtatt aaaaaaagtt ttgtaaaaat agcttctcac atttttacat aaaattgact
    26461 taatactgag aaactgactt ttgaacaccc tctatctgta aaaaaggggc tgcgacatga
    26521 tttttgtcac ggcccctctt ctttattcaa attttttcca aaacgttcgt tgataataaa
    26581 cgatccatag agcggaaagt attagaacta aaccgatcac aggaatattc actaaccaaa
    26641 atggataaaa caaagccaac attccgtata agacgataag aataatactg atgatcaatg
    26701 aacccatcat agttgcgact aaacccaaac ttcctgaacc tctgttaaat aattggttaa
    26761 tattggtcca gttcagcagc agcaaccgat aatctctagc gaaataacgt agtgacaata
    26821 agtagcagcc gaataaagaa ccaaatagca agctagggat aaaaaacaaa ggcagctgaa
    26881 aagacagtcc gcttaataat gcgatcccac cagttaaaaa actctgcaat atccatccga
    26941 atctgaactt ttcttttaaa tattggttca tagagattgg caaagaacgg ataaacagaa
    27001 aattttcttg atccaaagaa ataagattgc tgataaaaga agtctgatta actgttaaga
    27061 aagccaatgc gatccctacc aggaaaacaa caccgattaa cttattatct aaatcagtta
    27121 attttatttc gcctcctagg gcaaaagcaa gaataaaaat gattggagtt aacaaggaag
    27181 aagaaagaac ttgcataatc aaattaggtt cttttactaa ttgagcattg taactaaata
    27241 aaagctgatg cacattttgg ttctttttat gggttctttt tattttgaac tcactgtttg
    27301 aatcagtcag ttgctctgta agctttggta ctatcagctg ttttacagca aggaaaaatc
    27361 ccagagccgt gatagctagt attccccaac ccaataaacc ttgttgagaa agtggctgac
    27421 tggcagcatc atagaatggc aataatatcg aaatcggatg ccgatctgtt aactcatttt
    27481 caatagtccc cgtttgatga ttcatccaca tgattccagc aaatgcgatc agcatcgagc
    27541 tgccaagcaa gatactagaa actgtttttc tatgtttttt aaacactgct gttcgagcca
    27601 aaccaaaaac aatcaaacta gccagacaaa aaagaataat caaaaataga gaaaacaaaa
    27661 ggattgagat aatgatagta agcaaaatcc ctagatcagc tcgccatccg gttaaaataa
    27721 acatgactag aagcggaaaa acaaaaggaa tgatggtcaa agcaactacc aaaatctttg
    27781 ctgaaaaaac ctgttcttgt ccaaaaggca gaggcaaaaa tacttgcagg tcatttcctt
    27841 caaaaaagat attataaatg actgatatac tttgagttaa acctaacata ccaaaaagtg
    27901 ccacgtaata agtaaagaaa cctggcaatt taacaaagtt catagcaaac atagcaaatc
    27961 cgtaaatcag taaaaaaatc agccctgaaa aaagattttg agacaaaaga tagatagtta
    28021 atcgctttcc cttttttcct ttctctcttg ctttattcgt gagctgtgga ttaacatata
    28081 aaagattaac tcgaattaat tctaataatt gtcgcttata catacggtcc atctcctgtc
    28141 gcttgccttc cagccatttt caaataaatc ttttctagcg tttcatcagg tgagccattc
    28201 aataattcac taactgcccc ctgataaatc aattcacctt tcttcaaaat agccagttta
    28261 tcgcataact gttgtgcggt atccaataca tgtgtagaaa aaatgactgt ttttcctttt
    28321 gcggcatgag ccttcatcat ttctttcaaa tcaaatgctg cttgaggatc aagtccttgc
    28381 aaaggttcat ccaataccca aatatctgga tctggcagca acgtgccgat caaaatcgtt
    28441 ttttgacgca ttccatgtga aaaacttgcg attgtttcat tttgatgact atacatatca
    28501 aaaagcgctg tcaattctgc taaacgcttt tctttttttt gtgtgtctaa ttcataggca
    28561 gctgaaatca agtcccaata ttctccagcc gtcaattgta aaaaaatatc tggggaatca
    28621 ggcacataac cgattttttg tttgatactc aatcgatttt ccgtcaattt cattccatct
    28681 actaaaatcg ttcccgatga tggttcaata atactaacta aacttttaat cgtagttgat
    28741 tttccggcac cattatgtcc taaaaatcca aaaatttccc cttgtcttat cgagagagac
    28801 aagtctttta atgcttgttt tttcccgtaa cttttactta cattgatgaa ttcaatcatt
    28861 tctgtactcc ttttgtttgt tattactttt tattgtaccc ttcgttattg gtggagtaaa
    28921 tgtcaaaaag agtgcataac aaaaaaggat agaaacgact aggctgctac ttgggttgtt
    28981 ctcttctaca ggaaaacact cttctaacag gtaagatatg agtctttgtt ttctttaaaa
    29041 tgattcattt tctatctttt cttgcataaa attctttagt ttatcaatac ttctctttcc
    29101 catctctagt tgttctccat ttgcaaatgt tactatttcg tttactctgc taatagatgt
    29161 aatgttttca gtattaatga tataagcttt taaatcttta cataaatact catattcgag
    29221 tgatttttta tattttttaa ttgtgccgtt tacgagaatc tcttcatctt tcaatactag
    29281 gcctatttta tttttataac ctggcatgct cattagataa aggatttcat cgtaatggat
    29341 atagtgatcg ctattaccat aacggacagt tgtccacctt tttttcgtag catgttgaac
    29401 aggatagttt tctttttctt tcaataagta actttggatg ctttttttat ttttttcata
    29461 agtgaattct tttatcaaat aaccactagg cgtaatcttt cgattaataa cagttattgc
    29521 tttattcgga tgagacgtta gataaacaat tttacacttt tcattctttt ctctaagcat
    29581 ttcgcctagt tcgataccgc tataataggc gttcaaatcg atgtctagga aaaaaatatc
    29641 tgaggaatag atttcattat ttttaatgaa ttcaacaaat tgtataacgt tcaatccctg
    29701 aacgatctca aatttccaag gacttaataa aattctggat tcttccaaaa tttctactag
    29761 cattttttca aaaaccaaac tatcatccaa aataaatata cgcgttacca taaaaagtac
    29821 ctccactcga tattttacta tcaaaatcat ttcatacaac ttttcaacat acatgttagg
    29881 aataaattgt aattctcttc acaatctttt ccaacacctt tgaaaccgat atcaaatact
    29941 ttcaggatgc tgcttctaaa ataaatatca aacaaatgaa tacatcatgc aaaaaaagcc
    30001 agacaaccga cgtccttgcg tgtcagctgt ctgagctatg tttatttatt ttgttctttt
    30061 aaaaagaacg atacacatac tgatttttcg gagcagagac tttctcagcg tttgtaacaa
    30121 ctacagatgg gatttcacgc ttgaatggtt gataatagtc tgattggatg gttccttcta
    30181 ttttatacca ttcatcattt ttaaccgtta ctccttccgg catatgcgtc aacaaaccaa
    30241 agactcctga atctgccaca caatggataa tgccgaatcg gaacaagaag atatctgtat
    30301 tgctatcgtt cggtgtttgg taaacaaatc cttcatagct gatttttttg ccaatgaatt
    30361 cgctagggta attataaatt agctccataa cttctaaata gttttcatct gtgatcttga
    30421 tagtttgctt atccccatac ttttctttca actcattcat ttggtctaag tagtctgatt
    30481 tgttgaaata aatactggta tccggcttta agtattgtgt ctgcatctcc ggatccccta
    30541 ctgattcttt acttactggg aaattaaatc ctttggcttc tacgatcgta gtgtcgagac
    30601 taactgttgg aaacaacgtc cccacgatta ctggcaaagc tagcagtaca taagcgattc
    30661 cacgttgata aggttttgac aacccatggt catgatcgtg ctcgtgtgcc ttttcttctg
    30721 cttttccgtt tttgacccac aaatacagct gaactaaagc caaaagagca gataaaatca
    30781 ttgaaagaat agctaagtag cgataatgga cattgatata ttgatcgagt ttgccagaaa
    30841 cttgcaagta catcatcaat tcgaaatacc cagataaaat caaaaaccgc agcatatttt
    30901 ccctcctaaa taaataatgt gaatccaata acgctaaggg caatcagtcc gatcagcgat
    30961 aaaataaagc gtcctgtaaa atatcgtttc atcatcagta gatttttgat atcgaccatt
    31021 gggccaaaga ccaaaaaggc agctacaggt gctgtgccga acagacttaa caaagaagat
    31081 cctataaacg catctgcttc tgagcaaaga gacatcgtaa aggccaataa cagcatgatg
    31141 ataatggcta aaatctttgt tgagccgagc gttagcataa ttctagttgg taaataagtc
    31201 tgcatcgcag ctgcgattaa tccgccaatg atcaaataac gacctgtatc aaaaaattcg
    31261 tcgattgaat gtgtcaacac atgcccagtt tgatggatca gctgattttt tggttcattc
    31321 ttctcacttt gttcttcttc atggacatga tgatggtgat tttctgctac tttagacgtg
    31381 attttttctt tcaatacagc gtctttttga aaataggcaa gccagacacc aatcacaagg
    31441 gcaacaaaaa agctgcctat cacccgccag cttgccatct gccaagaatt gccaaatgcg
    31501 ataaaagtgg aaaaaatcac gattggattg atgatcggag cagtcagcat aaaagcaaac
    31561 gctgtgtgaa ctggtacgcc ttttttaaca aattgatgaa cgatcggtac gatgccgcat
    31621 tcacatgaag gaaagaaaaa tcctaagatc gaaccagtaa aaatagagaa gatacgattt
    31681 tcaggaagcc atctcttcac acgttctggg gtcaaaaata cttgtaaggc gcctgagata
    31741 agacagccta ataatacgaa aggtaatgct tcaataacga tcgataagaa aatcgtactc
    31801 atctgcaaaa ccgaatttgg aataaaagaa aacatgagtt tcctcctttt tatttctagt
    31861 tcattcgctt tgcaaatata cgcttttatt caaaaaaaac aagttgctct gtaaattttt
    31921 acacaactct tattttttac caaagcactt caattgactt tttctgttat tcccattaaa
    31981 ttaaggtctc atacatatca cttctatcat aaaagggaga ataaaaatga aacaaaccac
    32041 taacttgact gaagttcaag acatactgca acagtcatcc gttgccgtca tttatctttc
    32101 gatgcctaat tgcagtgttt gtcacgctgt tcgtccacga ctagaggaac ttctcacaca
    32161 ttatgatatt ccggcacttc atttggatgc atttgaaaca ccagaagtag ctagtaaatt
    32221 tgaagtgctg actgcgccag ttgtcctgat ttttcatcaa ggaaaagaag tctttcggca
    32281 agcacggttc atcgacttta aaaaaatccg ttatctacta gatgaactga cagcagaaga
    32341 tgattccctt tcttatgaag aaatcttccg aacaaaataa tcgttgtcca aacagaaaaa
    32401 agcgattgaa caagtgaaag ttttgccttc atttgttcaa tcgcttttta tttttattct
    32461 gctgtatttt cggaatgcgc atttgcgtca gaatgcaaga aatagatcaa ggtttgcaat
    32521 tcattcgtca aatcaacatt ttggatcaat acatctgacg gagcattcaa tcgagcagcg
    32581 gtaaagttca agattccttt gattcctgct tccgttaact gattgactac ttcttgtgct
    32641 ctttcagcgg gtatcgttaa aattgctact tcgatttgtt ggatcttgat ttgttctgac
    32701 atatcttcca ttggataaac ggggataccg tctacgatgc gcccaacgat ttcctcattc
    32761 acatcaaaag cacaactgat acgtatgcta ttactttgat ggaatttgta ttttagtaaa
    32821 gcacttccca agttccctac accgatcagc gcaacatttg tcaattgatc gtcgtttaat
    32881 gtctttgcaa aaaagtgcat aagatcttct acatcataac catacccacg ttttcctaat
    32941 tcgccaaaat aagaaaaatc tcgacggatc gttgcactat ctacttgaac ggcttcactt
    33001 aattcagttg aagacacttt tttctttcca gcattattta aaatccttaa atagcgataa
    33061 tacaaaggca gtcttttagc tgtcgccttt ggaataatat gatctttcac gaaatgacct
    33121 ccacgtattg tctaattaca tctttgattt ctccatcata gcagtattta taggaaaaaa
    33181 tcaattccat tgctcagata ttgtgaaatt ttattcttta ttcatttatt tatcatttct
    33241 gcaaatctcg cagtataatg gaatttatgg tagactacta attgaaaaat agttttttat
    33301 ttgaggagaa ttcacaaagt agaaaagagg attccatgat attattacaa gccaatcaga
    33361 ttgctcgcca ttttggtgca gaagtcttat ttgaaaacat acatttagaa atcagtacag
    33421 gcgctcgtgt cgctttagtt ggcagaaatg gcgctggaaa atcgacttta ttaaagatca
    33481 ttgctggaat tgaagcacct gatgaaggaa agatcgctaa aaataaaaca gcgactctcg
    33541 gatacctcgc tcaggacaca ggacttgatt ccaatgaaac agtttggaac gagatgctga
    33601 aagcttttga acaagttaga caaatggaag agcggatgcg cgagctagag attttcatta
    33661 gcgaaggtac gccagacaca cctgcttata gttcttattt aaaagaatac gaccggctgc
    33721 aacacgattt ttcagatcga aacggctatg gttatgaaaa tgaaattcgt tccgtattgc
    33781 atgggtttaa attcgattct tcattttatg atcagaaaat cgatacttta tcaggcggac
    33841 agcgcacacg tttagcttta gctcgcatgc ttttgcaaca ccctgacatt ttgattctag
    33901 atgaaccaac gaaccattta gatatcgaga cactttcctg gcttgagaat tatctgcaaa
    33961 gctatactgg tgcattattg attgtttccc atgaccgtta ttttttagat aaagtagtca
    34021 atgaagtcta tgaattaagc agaagaaaaa tgcgccatta caaaggtaac tattcgaaat
    34081 atctagattt gaaagcagaa cagttagcaa gtgaatggaa ggcttatgaa aagcagcaaa
    34141 cagaaatcca taaattagaa gacttcgttg ctcggaacct tgttcgcgcc tctaccacaa
    34201 aacgagcgca aagccgcaga aagacattag aaaaaatgga ccggctggat cgtcctcaag
    34261 gggatgaaaa acaagcaaac tttctattca gtatagaaaa gccttctgga aatgtagtct
    34321 tgcaaatcga agatgccgct atcggatatg ataatgtcac cttatctgaa ccaatcaatc
    34381 tagatatccg tcgtcaagaa gcgattgctc tagtaggtcc gaatggtatc gggaaatcga
    34441 cgttgctgaa atcaatcatt caccagatcc cttttatccg tggaaaagaa acgctaggga
    34501 caaatgtcac catcggctat tacgatcaag gtcaagctga tctgcataca aacaagacga
    34561 tcctagcaga attgtgggat gagcatccta ccactccaga aaaagagatc cgaagtgctt
    34621 taggtgcttt cttattttct ggcgaagatg tagaaaaacc aatccctctg ctaagtggcg
    34681 gtgaaaaagc acgggttgct ttagccaagt tagcgatgaa caaagaaaat tttttgattt
    34741 tggatgaacc aacaaaccat ttagatattg acaacaaaga agtattagaa aatgcattga
    34801 tcgattatga cggcacactg ctttttgttt cccatgaccg atatttcatc aatcggatcg
    34861 cgacaaaagt cgtcgaactc tctgaaaatg gcagcaaagt ctatctagga gactatgatt
    34921 attatttaga gaaaaagcag gaagaagaag aaatcgccga attgttggca caagatgagc
    34981 cagaatctgc tcctgctccc acgaacaaat ctgattatta ccaaagcaaa gaacagcaaa
    35041 agcttatccg tagccttcaa cgaaaaatta ctcaggttga agaagaaatg gcgcggatcg
    35101 atgaatggat cgatcagttg gaacaagaaa tggtcgcgcc agaaaacctg gaagatcacg
    35161 taaaactcaa tgagctgaac caagatttag aagctgctag acaagatcaa gaaacaaaat
    35221 tgacggaatg ggaagaacta agcttggaat tagaagaata tgaaaattaa cgaaaaaacc
    35281 aaaacagaaa ccattcatgc tgttttggtt tttctttttt tattttatca aacaaaaatt
    35341 ccatatttac ctttttcacc aataagaaga ccaattacta gtcattctca ctaaagcttc
    35401 taaataatag taatcacccc aaatacaagg tgtatttacc ccattatttt tgggcttatt
    35461 ataaacagac tgtgttaaaa agccatcttc ttcttccgtt gtagcatatc tttctttcaa
    35521 ttgtcgcata attttccaaa cagcagactg gtataagttc ttttctgttt tgtctaatag
    35581 acctgattct attaattcta ataatccaca aaccatgatc accatactag atgtatcttt
    35641 ttcttctctg ctagaatcat caaagatcaa atcccaataa caaatggtat cttctggcaa
    35701 ccgattcata aaataattgg ctaatatttt gcttaaatca aaatattttt gttctttcaa
    35761 ataacgataa cttaatgcaa aaccgtaaat cccccatgct tggcctctag cccaacagga
    35821 attatcacta taaccttgat gcgtacttcc atatcttggt tttccatctt tgacatccat
    35881 ataaaatgta tgataagttg aaccatcttc tcttactaaa taatctgctg cattttgaat
    35941 atgagcctga gcagcttttt tatatttttg atttccagtt tcttcactcg cccaatacaa
    36001 taatggaaga ttcatgcaac aatccatgat cattctacct tgttgtgaag gatcactagg
    36061 atctccccaa gcttgtataa ctcctgcctt ctcccaaaaa cgctgaagta atgtttctgc
    36121 agcatcaatt gaagcttcct tcatttttcg atttcccgtt attttataat ctgctacggt
    36181 tgataaagaa tacaaaaaac caagatcgtg atgattagtg ataatttgac agtcaatcct
    36241 gtggataaaa tctatatctg ctttttcagc tgtttgttga aataaaggat cacccgtctt
    36301 ctcaaataac aaccaattaa ttcctgtcca aaatccttct gtccaaccaa tattgtctct
    36361 taatggataa cgtcctttta taggacactc gcttgggaat tgatactgac tgttactgat
    36421 acgtttgatt aacctctccg tgtgtctgcg acaacgctcg atctcattat tccattcttt
    36481 ttccattaat tctttataac attcatttat ccatttttct ggatgtatta tctcttcaaa
    36541 catcacttta tcaagcattt ccccaccagc ctttagcata attttagtct ttttttattg
    36601 tttttatttt ggatagttag cccggttgtc ccatcaaaat caattttggg acgattgttc
    36661 catacttcta aactataatc ccgatcttga tgagcaacca ctcctgttcc gagccaatgg
    36721 gttccttttc caaatgtacc ctctaatcca ggaataaaag tgttttccca tgtcatcaag
    36781 tttgtattgg gcaaacccat aatagctgtg gattttcgtt taatagtatc gtcacttaaa
    36841 cagataatac ctgaaaagcc agcttcactc accctatagt tttccttgtc ttcttctatg
    36901 attattcctt ctttctcagg agaacattca atagcaaacc ctgtctcata tagaacatat
    36961 tctgtatcta attctacttt gtgtacgcga atctgccagc cttcaaaagg aataagccaa
    37021 gttctgattt ttgcctgtaa agtaggttcc catgtagagg caagtacctc tttcttacat
    37081 acaatgtctc gtacattatt tcgaacgatg aactgtccat aggttttgat gctctcattc
    37141 ttaaaactgg tcgtgatata tgtgtccgct cgctgaatcc ctagcattga atccattgcc
    37201 atttctgctt tcaattgatt cgctcgcggt atcgaaaaac caaattgact agaataagca
    37261 aattttaaat atttttcatt ggtcaatgta tggaaatttg gccctaattg tcccgcattc
    37321 aaaaaaaatg tatgtccatt atcatgttgt atgagcaaat tggcttctga aagaagttta
    37381 gttccttcac gaataggcat aggcatttct ttagccgtcc aatactcatg gtcttcaggt
    37441 agcgccaaca ataaaaaaat cttattcagc cataatggag ataacgtact attatagggt
    37501 tcagtcaatg tcaattgagg gtaggtataa ccaatattca ggataccttt ttcatcaaat
    37561 atttcttttt gcatccacca tcttatattc cgattgacta accccttgat ttcacctaat
    37621 ttaaaaggca aaagatcggc ccacaacata gctgaccaaa agcttgaaac tgcaaaacga
    37681 taaatcatgc ttcgaccaaa aggcacattt gcaccatctt ccgcaaaaaa atgaatatag
    37741 tctctagaaa aatcaatagc tcggttctta tatatctggc taatgggctt ttctggttct
    37801 agtacgctat aaatcaaacc ataataatga aatgcaaaag gattataata atcttggcga
    37861 cctcttgaac tgtctcgata ccagccattt tgaagataac aattttctat attatcaaat
    37921 gcttctttca gtgctgattc tttaactgga taaccaaatt tttggataat tgttccgaca
    37981 attatcttaa aaaattgcca attaccatct gcacattcat attcaaaaat ttgcgagaac
    38041 caatgaataa tctggctttt ttctcctgta tctaattttt tccataataa ttgttcatga
    38101 tgatataacg ctaataacat tgcgggcatt tctaccatac gctggtctct ttcatgtatt
    38161 tctccccaat aatcgggatg ggaaggatct gttccagctt ttattcctga tagaattaat
    38221 ttcaaccatc gttcatctaa ttggtcaatt gcgggccccg ctccccataa aacccgagag
    38281 aatccttcca ttcctgcaat tttatttcca tatcctaccc cttgatgtat ataatcaatt
    38341 tgtgcgccac ttgctcgata aaacggctgg agtggttgat ttatcttttt aaataagtca
    38401 aagtaatcct gtctggtcct taatagtggt actggatgtt tcattgtatt ttctcctctt
    38461 tttactacca aatagctaat tccgactgtt ctaatcgaag taagccttca ataaaaaaat
    38521 aatcagaata gatcagtggc acattataag tcagttctct atggtatgct tctgctccgc
    38581 ctccaatgat cccatcttca tccatattcc aatttgcata gtgcttttct gtcttttgga
    38641 gtatattgat agcagcttca tgatacattt ttgatcgtac agaggaaaca tatcctgcta
    38701 attccagaag accgcatgca gcgcatactc cagcactggt atcaatattc gaatctttta
    38761 cagcaaaatc aatgagagga atataaccag ttttttcgat tttctgaata aaatttactg
    38821 ctatcttttc tgctatagct agatagtacg gattttttgt gtaacgataa cttaacgcga
    38881 atccatataa agcccatgca tttcctctgc tccaacaaga attctcattg tatccttgcc
    38941 cgccaagtaa ccgaatatat ttccctgatt ctgcatcaaa ttctgcgata tgcccggttg
    39001 atccatcttt cctcactaga tacttagctg ttgtatctgc atgctgccga gcaattttag
    39061 agaacctcgg atcaccagtt tccctcgaag cccaatacag taatggtaaa ttcatcatac
    39121 tatcaataat catccaacca ggtttatggt cattccatgc tactaaatag ttccctgaaa
    39181 tattatagcg tccagcaagt accgtagctg catgtaaccc tagttgtcga ctatgtttgt
    39241 tttgagactg ttgataatgt gcaatagccg atggtaagaa taaaaaacct acatcatgat
    39301 gaacgtctga aaaatggtac agggcagatt ctaataattg ttcttgttgt attgctgcct
    39361 ctttgaactt ttctcgtttt gtcttgtgaa agagctgcca taaaatacca ctccaaaaac
    39421 cattggtcca ccagtcgatt cctctgtcct ttattacgtc tgtatatttt ccgttgtaag
    39481 aaatatatgg aattttattc cccattctgt ctaattccgc atctatcttt tcagaagcta
    39541 cattcaaaat ttttcttgac cattccttcg tctgttcatt catggatatc ccgccattct
    39601 tttaattttt atgaatgtgg agttgctcct agaacgaact agatggttaa cattcgttat
    39661 tttttttact tatttgcagc tccaatgtca ttctccttaa atttagactg cgcccacaca
    39721 taaatcaaga ccattgggat cactgaagtt gctaaaatta gcataattgc gccataatta
    39781 attggctcac ctgtactaga aaccgtcata tttttgataa ttaagggaac cgtatattta
    39841 tctttatctg aaagcatgac taatggaaat agatattgat tccaacttcc cataaagata
    39901 agtaaaccac tagtaataat cgtaggtgat aaaatcggta cagcgatttt cgtaaaaatc
    39961 acccactcat tggcaccatc gattctggca gcctccatca gtgctgtagg aaattgactg
    40021 ctatattgtc gcatcataaa cactgaagtt gtactggcaa tcgaagggat gatcacacct
    40081 gcataagtat tcgataatcc taaacgactc ataatgtaaa ataaaggaat caatgtggta
    40141 aaaaaaggca ccatcctaga tagcataact gtattgaaca acagtttatt ccccataaaa
    40201 cgatactttg ctaatgcata acccgccatt gtagaactgg ctgtagctaa aactgtactg
    40261 attacagcaa taacaatcga attccaaata atcaagggat aattatatct cgccatgatg
    40321 gcttgccagt tttttaatag catatcccca aacttgaaat tgattccgcc acttaaaatt
    40381 tcttgattac tttgcgttga agctacaatc atatataaat atggcatcaa aaatattaag
    40441 gcaccaaaca caataatgac gtacgtaaaa attctgcttt gtatcttttg tttagtcatt
    40501 tttatcacct ccgaatttaa attgaatagc cgataatata acagatacaa acaccatcgt
    40561 ccaggcaata gcagaggctt ttccaaaatc aaccatttca aaacttgttt tatataaatt
    40621 gactcctacg accaaagctg aattttctgg tccattggaa gtcccaaaga atatatttgg
    40681 gatattgaaa ttacccaagc cattgatagt ggctaaaact gtggccatta aaatgattgg
    40741 ctttagcatg gggatcgtta ataaaaagaa ttgccgaaat ttcgatgctc catctatttc
    40801 agcactttcg tatatctctt tagaaatccc ttgaagacca gctaaaaata aaatagtgtt
    40861 atatcctaca ctaagccaaa tcatcgtgat gatcattgat attcgtatcc aaaaaggatt
    40921 tgttaaccac tgtacaccgt taattcctag cgcattcaaa aatccattga ataacccagt
    40981 cggattgaaa aaagtcatga atacggaagc tacagccacc gttgatgtaa tggttggaat
    41041 ataatagaca gttctaaaaa atccttttcc ttttaactgt gctgaattca ataaaacagc
    41101 aaagtaaata gctaatagca tcgttattgg aattgatcct gccatgataa tcaaaacatt
    41161 cgtgtaagat ttaataaaca atggatctcg gaagatagct agataattgt caaatccaac
    41221 aaattcaaat gagtagccaa ttttcataaa actaaaagca aacgagataa tgatgggaat
    41281 caaaatcgtc acaataaaga aaataacaaa aggtgtgacc aatccataag cacttttttt
    41341 atgagacgta tctgttgctt ttttcttgct gttctttatt gtcttgcttg gtaacgaaga
    41401 ttgcatcatc gttaagtcct cctaatattt tttaatcacc agttaatttt acgccgtact
    41461 tatgagcaaa attttctgct tcttttagat atgaattttc aaaatcacct gtttgccaat
    41521 agttatatgc agcggtagtc aaataagacg tagcatctga ataagtggct ggtaaaacag
    41581 tatttgatgt attttcattg attttattca atagttcata gtatttttgt cctccttagt
    41641 agtcaaactt tttctcagct gcttctgttt gataggccaa tgagttagat gtagccacgc
    41701 ccagctccag tgcgttggct aagcattctt catctgtcag cataaatgta agaaattgtt
    41761 gtgccacttg tggatttttg gcatctttag ggacataaaa acttgatcct cctgatactg
    41821 caccttttcc tggattttct tttgaaaaag gaatcagtgg agccatgcgc catttgtcag
    41881 atgcatcagg aaaattcaaa gacatattcg tagcatacca gccgccagca acaaacattg
    41941 cagattcttg aaatgctttc tcagcatctt gtgcaccgta gaaatgtaca atatctgagt
    42001 cgatcatttc tttgattaac tttgcagcat gtttcgcttc tttagtattc agacggatat
    42061 ttccttcttc atcaagcaaa gattgattct gctgggctag catgttgata aacaattctt
    42121 ggtccccaga ttctggcaag gcaattccaa aactaccagt tttttctttt aatgcttttg
    42181 ccacctggat atattcttcc caagaactga tttcttcaaa atctacacct atttcagcaa
    42241 atttttttgg ttgataataa caaagtgctg gggtaaaatc actagcccaa ggaataatcg
    42301 tttcattact cgtttgttga cgcaaaatat tcaatttttg atcataaaac tgatcgccat
    42361 atttgtcata aaatccatat tcatcagcac tataaaaagc atcttgaaat ttttccagca
    42421 cacctgctgc tgaagtgtct gccatggaga taatatccgg catatcttcg tgtgttacca
    42481 attttggggt gatcatttgt actgttgctg ttcccgaagc tacattcaca atttttaagt
    42541 caatatttgc tccagttttg cggttataga tttttgcagc attttcaaca tagtttgtca
    42601 gtttgccgtt tgcgacatat acttctaatg tagtggcttt ttttatcatt ggatctgcga
    42661 tgtttgctgt tacttcttcg ttagtttttc cacatgcact taagctgaat aatagggtaa
    42721 tgtttagcaa agctatcacc aatttgtatc tttttttcat gtctatccct tctttctttt
    42781 caagttctta tataaaacgc tttcacttat gtatattaac ggaattagta acacaaatat
    42841 ttatataaaa aaatatttta cttg
//
LOCUS       DAZUMM010000015        42415 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000015 DAZUMM010000000
VERSION     DAZUMM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.015"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG8E6_000738"
     CDS             476..1339
                     /locus_tag="ACG8E6_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5061181.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG8E6_000739"
     CDS             complement(1468..3090)
                     /locus_tag="ACG8E6_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5061182.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG8E6_000740"
     CDS             3339..3860
                     /locus_tag="ACG8E6_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS5061183.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8E6_000741"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8E6_000741"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFS5061184.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG8E6_000742"
     CDS             5941..6759
                     /locus_tag="ACG8E6_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFS5061185.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG8E6_000743"
     CDS             complement(6862..7059)
                     /locus_tag="ACG8E6_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061186.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG8E6_000744"
     CDS             7228..7806
                     /locus_tag="ACG8E6_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS5061187.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG8E6_000745"
     CDS             7901..8038
                     /locus_tag="ACG8E6_000745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061188.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="ACG8E6_000746"
     CDS             complement(8851..9354)
                     /locus_tag="ACG8E6_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS5061189.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="ACG8E6_000747"
     CDS             9713..10738
                     /locus_tag="ACG8E6_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5061190.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11061..11939
                     /locus_tag="ACG8E6_000748"
     CDS             11061..11939
                     /locus_tag="ACG8E6_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFS5061191.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12415..12531
                     /locus_tag="ACG8E6_000749"
     CDS             12415..12531
                     /locus_tag="ACG8E6_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS5061192.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13459..13797
                     /locus_tag="ACG8E6_000750"
     CDS             13459..13797
                     /locus_tag="ACG8E6_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061193.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13891..14121
                     /locus_tag="ACG8E6_000751"
     CDS             13891..14121
                     /locus_tag="ACG8E6_000751"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFS5061194.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14521..14892
                     /locus_tag="ACG8E6_000752"
     CDS             14521..14892
                     /locus_tag="ACG8E6_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFS5061195.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15166..15858)
                     /locus_tag="ACG8E6_000753"
     CDS             complement(15166..15858)
                     /locus_tag="ACG8E6_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5061196.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15861..16646)
                     /locus_tag="ACG8E6_000754"
     CDS             complement(15861..16646)
                     /locus_tag="ACG8E6_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5061197.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16883..18283
                     /gene="lacG"
                     /locus_tag="ACG8E6_000755"
     CDS             16883..18283
                     /gene="lacG"
                     /locus_tag="ACG8E6_000755"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFS5061198.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18308..18625
                     /locus_tag="ACG8E6_000756"
     CDS             18308..18625
                     /locus_tag="ACG8E6_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5061199.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18638..20311
                     /locus_tag="ACG8E6_000757"
     CDS             18638..20311
                     /locus_tag="ACG8E6_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFS5061200.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20325..21956
                     /locus_tag="ACG8E6_000758"
     CDS             20325..21956
                     /locus_tag="ACG8E6_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFS5061201.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22828..24018
                     /locus_tag="ACG8E6_000759"
     CDS             22828..24018
                     /locus_tag="ACG8E6_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HFS5061202.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            24152..26341
                     /locus_tag="ACG8E6_000760"
     CDS             24152..26341
                     /locus_tag="ACG8E6_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5061203.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            26338..28131
                     /locus_tag="ACG8E6_000761"
     CDS             26338..28131
                     /locus_tag="ACG8E6_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS5061204.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            28331..28645
                     /locus_tag="ACG8E6_000762"
     CDS             28331..28645
                     /locus_tag="ACG8E6_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5061205.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            28666..29043
                     /locus_tag="ACG8E6_000763"
     CDS             28666..29043
                     /locus_tag="ACG8E6_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5061206.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            29053..29826
                     /locus_tag="ACG8E6_000764"
     CDS             29053..29826
                     /locus_tag="ACG8E6_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061207.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            29848..31251
                     /locus_tag="ACG8E6_000765"
     CDS             29848..31251
                     /locus_tag="ACG8E6_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS5061208.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            31433..32617
                     /gene="mobT"
                     /locus_tag="ACG8E6_000766"
     CDS             31433..32617
                     /gene="mobT"
                     /locus_tag="ACG8E6_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS5061209.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            32614..32904
                     /locus_tag="ACG8E6_000767"
     CDS             32614..32904
                     /locus_tag="ACG8E6_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061210.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            32901..33122
                     /locus_tag="ACG8E6_000768"
     CDS             32901..33122
                     /locus_tag="ACG8E6_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061211.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            33164..33943
                     /locus_tag="ACG8E6_000769"
     CDS             33164..33943
                     /locus_tag="ACG8E6_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFS5061212.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            34004..34504
                     /locus_tag="ACG8E6_000770"
     CDS             34004..34504
                     /locus_tag="ACG8E6_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS5061213.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            34560..35198
                     /locus_tag="ACG8E6_000771"
     CDS             34560..35198
                     /locus_tag="ACG8E6_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFS5061214.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            35270..35665
                     /locus_tag="ACG8E6_000772"
     CDS             35270..35665
                     /locus_tag="ACG8E6_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5061215.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            35649..38102
                     /locus_tag="ACG8E6_000773"
     CDS             35649..38102
                     /locus_tag="ACG8E6_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5061216.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            38099..40126
                     /locus_tag="ACG8E6_000774"
     CDS             38099..40126
                     /locus_tag="ACG8E6_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS5061217.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            40123..41145
                     /locus_tag="ACG8E6_000775"
     CDS             40123..41145
                     /locus_tag="ACG8E6_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFS5061218.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            41162..42091
                     /locus_tag="ACG8E6_000776"
     CDS             41162..42091
                     /locus_tag="ACG8E6_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5061219.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            42336..>42415
                     /locus_tag="ACG8E6_000777"
     CDS             42336..>42415
                     /locus_tag="ACG8E6_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="regulates the transcription of tet(M) by
                     attenuation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HFS5061220.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKS"
BASE COUNT        13885 a         7039 c         8377 g        13114 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacgctt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat
    10921 aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata
    10981 gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg
    11041 gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga
    11101 aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag
    11161 agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg
    11221 aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag
    11281 aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa
    11341 tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta
    11401 gatttattca tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga
    11461 tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat
    11521 attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata
    11581 ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc
    11641 gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt
    11701 tactatcacc caatcttact aaatggttga aaaaagagtc aaaaatctgt ctttttgcag
    11761 ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct tggatctttg
    11821 gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa
    11881 aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag
    11941 tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc
    12001 acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa
    12061 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc
    12121 ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac
    12181 tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa
    12241 cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt
    12301 ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt
    12361 tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa
    12421 aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa
    12481 cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga
    12541 aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg
    12601 tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta
    12661 gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa
    12721 ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa
    12781 cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg
    12841 aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt
    12901 catgaaatat ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata
    12961 agcagttacg aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa
    13021 aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg
    13081 ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa
    13141 taataagttt tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag
    13201 taataaagta tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa
    13261 ttaatggtta aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg
    13321 tgatactaga ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa
    13381 tgaaaaagaa gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt
    13441 agccaagtaa cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt
    13501 cgtgctacag aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct
    13561 agtgagccaa ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt
    13621 atggtggtaa ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt
    13681 gaaaaggtga gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc
    13741 attttaacgg attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca
    13801 ctagtgaaat actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa
    13861 tcgatcatca aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt
    13921 tattacgtga atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt
    13981 cgctactcaa ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa
    14041 actgtttttt ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat
    14101 tatgttagta tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt
    14161 tattgttttt tcgatatctt tgctgttaat attactaact ttaatgcagt ataaagagta
    14221 tccagagtat tttattttaa cttttgtata tttagtaagt agctacacac ttctatataa
    14281 attcagaagt tcttctagag tttctaaagt ttgtttctta attagcttat tgattctata
    14341 atatttttga tttatatagg aaattaaaaa tattatagaa tcaacactca gagaacgggt
    14401 tggtttttaa tagatttaat aagtactgat aatgaaattt ctcctggttg ggaaaacgaa
    14461 gttggttacc ctgtagaaga attttttcaa gattactttg cggagtatgc cgataggata
    14521 atgagagatt acgtaacaga taaaactcaa gaagtcatca atcaatccca gtcattaaat
    14581 gaattatctg ataaggcgaa agaagaagca gttgccatta tttcttcagg gattagtgac
    14641 tatattgata tgagtggtga aatcaattat ggcgataaag acaaaattgt acgcatgata
    14701 aaagctttta tcaaaacttt tgatgtgaag gcagatctta agtttaatga tacttaccta
    14761 gtaggaaaat taattaatat ccttgggaca gagaaagata cggaaacgtt tatttcctct
    14821 ttgtctatgt tgtttacaga taagcatttt tcaactcagc ttggtggaga ggaactagtg
    14881 gaaatctttt aaccattacg cccagcatta ataaaagtat atcaaaaaag tactcctgat
    14941 gatttttaag attggtttga gaaataatgt gaaaacacta tttttaatgt taattataga
    15001 tagagatgaa cgcgtcatat ggttaaaaga atgaaaaagt caaagataca ctgctatatt
    15061 tcactaagga tatagcagtg tatctttcat gtcgtttcac tggcagtttt ttataaaagg
    15121 aggtgtgcat ttcagttgct tttctttggt agttagttga atcgtttaag aattaacgga
    15181 tgtggcagac aggtgctctg tccaaaaacc agtaaaatct ttgatggttc catctttatt
    15241 tagctgtgcc tgttcaaata ctgttttgtt tttaccgaat ggatcaattg caataattgt
    15301 tccgatcccc gcacgtttgg cagcaagtaa tcctgagtaa gcatcttcga taacaagaca
    15361 atcagccgga gctaagctca gtttgtctgc cgctttaaga aaaatatccg gttgcggttt
    15421 cccagggaaa gagccgtcgt catagacaat cttgtcccag tcaaaccagc gggctaaatc
    15481 gaaaatatca aaataaaaag caacattctc tttgacagta gccgtggcaa tcgttaatgg
    15541 aatctgttgg tcagtcagct ggtcaagagt cgcttcaagc ccttttgtca actgaagctc
    15601 gttttcattt tgcaagcaaa gcatacgata atagctttct ttttcttctg acaatacggc
    15661 gatctcagag gcggtcaaat gctctgaaat gaaatgtttc aaaatctccg tattcgtccg
    15721 accatgaata ttttttaaaa tatcctcgtc tgataaacca tcttttgtat gatcatgtat
    15781 catatgcagc caagcagctt catgcagatg agagtccaaa aacattgttc cgttgaaatc
    15841 aaatataatc cctttcatag ttacgctcct atagtggtat tttcggttga atcagcaatt
    15901 tgcagatcga catattgtgc atagtgctct tgaacctccc gcgagatctg gcgatccgta
    15961 atgagcagat cgaaatcatg caaattataa aacacataaa aatcatctcg attgaatttt
    16021 ttattatcaa cgagtagata tttcttacgg gaattattta gagcgatctg ctgtgcttct
    16081 ccttcttcgg tactgtaagt agagatcgct tcgttgtgaa cgccatttga actgataaat
    16141 gcttttgtaa actttagtct tcttaaattg tcgttggtga tcggtccgac aaatgcgccg
    16201 gtatttttcc ggaaatcacc gccaatcagt agaatttctg gtccatctgc ttgagcaaga
    16261 atatcaaaaa ctggataatt atttgtaatg atccgaattt tcttgttcag taaatgcgtc
    16321 gccaatagtt cgattgttgt acctggtcct aagaaaatcg tttctccctc gtgaatcaag
    16381 gaggctgctg tggcagcaat ttcttttttt tcttgaatct gcagagtggc tttttcagtg
    16441 tgagaaagtt cttgatctaa agtgtaggaa agactttgag cccctccatg gacacgcagc
    16501 agcttgcccg atttttctaa ttcatctaaa tctctgcgaa cggtcatatc agaaacatct
    16561 aattgattga tgatttcatt aacggtaagg attccttttt tattgaccat ttctacgata
    16621 gtaagcaatc tttctttttt caacattgtc ttcacctcta atttaaggat aatctatcat
    16681 ataaacggac aacagtcaat ctaattcgaa caaaagtaaa catttagggt ggtttttatg
    16741 tttgttacaa tagattttat tgctgttttc tggtttttat gattatttgt gtttgttttt
    16801 gttgacaaat gttttggatg cgtttacaat gcaatcagtt agaaagagcg aaaactgatt
    16861 atagatttga ggagagatgt acatgcttaa attaccagaa gattttattt ttggcggagc
    16921 cacagcagcc tatcaggttg aaggagccac taaggaaggt ggcaaaggcg ctgttgcttg
    16981 ggatgacttt ttagaggaac aagggcgatt cagtcccgat ccggcaagtg atttttatca
    17041 tcaatatgca aaggacattg aattgtgtga acgttttggt gtaaatggtc taagattatc
    17101 cattgcttgg agccgcattt ttcctgatgg agctgggaaa ccaaatccag aaggaatcgc
    17161 tttttatcat cgtgtgttcg aagaatgtaa gaaaagaaac gtaacgccgt ttgtgacgtt
    17221 gcatcatttt gataccccta aacgtttgtt tgaccatgga gattttttga atcgagaaac
    17281 aatagaagca tttgtttcct atgccatttt ttgttttcat gaattcaaag aagtcaaggt
    17341 gtggagtacc tttaatgaaa tctatccagt tgcaaccaat caatatttgt taggtgtttt
    17401 cccaccagga atcaaatatg attttacaaa gatcgtcgcc tgtcttcata atatgatggt
    17461 tgctcatgcc cgtgtagtga actatttcaa agaaaacgaa cttccgggtg aaattggtgt
    17521 agttcactca ttagagacga aatatgcggc aacagacgcg ccggaagata agcatgctgc
    17581 ctttttagac gatgcattgt cgatccgttt cttattagat gctacctatt taggctatta
    17641 ttcaactgaa accttgactg ctcttgatga gatatgtgaa gcaaatcagg ccagctatca
    17701 ttttcctgaa gaagatttcg ttgaattgaa aaaagcatcc acgcgtaatg actacttagg
    17761 gatcaatcac taccaatgcc attttgtcaa agcttacgat ggagaaaatg caatccatca
    17821 taacggtacg ggtgaaaaag ggacctctgt ttataaagtg aagggaatcg gagaacgtat
    17881 ttataaagaa ggaattcctc gaactgattg ggactggctg atttatccag aaggtctata
    17941 tgacttgttg ctgcgaatta aaagcgatta cccacattac aacaaaatct atatcactga
    18001 gaacgggatg gggtataaag atcagtttga agatggcatt attatggatc agccgagaat
    18061 cgactatttg agagtctatt tggaatcctt aagcaaggcg ataacagccg gggtaaatgt
    18121 gaaggggtat tttttgtggt cgcttatgga tcttttctca tggacaaatg ggtataacaa
    18181 aaggtatggc ttgttttatg ttgattttga aacccagaag cgttacccaa aagaatcagc
    18241 ttactggtac aagttggtca gtgaaaccaa aacgattatt taataagtga taatggagga
    18301 agaaaagatg aataaagagg aattacaaat gcttggtttt gagatcgttg catattcagg
    18361 cgacgctcgc agtacattat tgaaattatt gaaagaagta agacaaggaa actttgaaca
    18421 tgttgattcg gcgttgaaag aagccgacga aaatttaacc cttgcacata attcgcaaac
    18481 aaaaatttta gcggaagaag catctgggaa agagatggag atgggattca tttttatcca
    18541 tgggcaggat catttaatga ccactttatt gctgagagat ctgattcaag actttattgt
    18601 actgtacaga cagaatcaag gataggagaa gaacgctatg gatggaatca ttaaacaaat
    18661 cgaaaaaggg aaaccatttt ttgaaaaaat ctcacgtaat agttatttag gagccgttcg
    18721 tgacggattt ttaacagcga tgccagctat tttattttca agcattttca ttttgattgc
    18781 tgcggttcct gagatttttg gttaccaatg gcctgaatct gtctccacat ggctatggaa
    18841 agtctataat tattcgatgg gtgttgtagg gttactcgtt tcagcaacca ctgctcgctg
    18901 cttggctgat tcgatgaatc gtcgcttacc gaaaaacaaa gtaatcaaca ccacctctgt
    18961 gatgctggcc tctgtggtaa gtttcctgtt gttaagcgtc tctccgattg aaggtggatt
    19021 ttcaacggaa tatatgggga caaagggact actggcttcc tttgtcgctg catttttaac
    19081 agttaattta tataagtttt gtgtgctgaa agatattacc attaaaatgc ctaaagaagt
    19141 tcctggaacg atttcgcaaa ccttccgaga tatttttcct ttctcttttt cagtatttgc
    19201 ggccgtttta attgatttag gcattcgaca aatcttccaa atctcatttg cagaagcaat
    19261 cattacggtg ctgcagccgc tatttacagc agctgacggg tacttaggaa tcgctattat
    19321 ctggggagcg atggcgctgt tttggtttgt cggtgttcat ggaccgtcga tcgtggagcc
    19381 ggcaattgcg gcaattatct atgccaatgt ggaaaccaat ctgcaattgt tcaattccgg
    19441 tgaacaagct tctaaagtac tgacggtggg tttagggaat tttgtaggaa ctatgggagg
    19501 aactggggca acattggttg ttccttttat cttcctactc tttgctaagt ctaagcagtt
    19561 gaaggcagtt gggaaagctt cctttattcc agtaagtttc gctgtcaacg aaccattgct
    19621 atttgcggca cccatgattt tgaaccctta tttttttatt ccatttttgt taacacctgt
    19681 tgtaaacgtt tgcctgttca agttttttgt cgatgtattg ggcatgaaca gctttatgta
    19741 cgttttgcct tgggcaactc cagcaccgat cggtttggtt ctaggaacgg gcatgggtat
    19801 cttagctttt gttctagcag cggttctaat cgtcgttgac ttcctgatct atctaccatt
    19861 ttgcaaagca tacgatgctg tcttagtaga gaaagaagca caaaaagcag aggtgatcgc
    19921 aaccgaggaa ggtgcttctg ccgatgtacg tgaggaagta cctgtggcag cgagtcaagt
    19981 tatcagcgat gcaaataaag aaatcaatgt gttggtactt tgtgctggcg cagggacaag
    20041 tgcgatgtta gctaatgctt taaaaaaagg tgcatctgaa tcgaaagcac cgatcagtgc
    20101 aatggcgggt gcttatggtt ctcattacga aatgctgcag gattttgata tggtggtctt
    20161 agcaccgcaa gtcaattctt actatgatga tatcaaagaa gacaccgatc gcttaggaat
    20221 caagctggtt gcgacaaaag gagcagaata tatcggatta acgagagatc ctgaaaaagc
    20281 tgttcagttt attctgaacc aatttcaata aaggaggagc cataatgaaa atgggaattt
    20341 ggttgcgtaa agctgtttta tttgcagctg tgttaggggc tttgggggct gtttcaaaaa
    20401 ctgttgaagc atctgccagc gaatttattc gcggagcaga tatttcgatc ctgtcagata
    20461 tggaaaaatc tggtgcaaaa tattatgaag acgatatttc aaaagatgcc ttagagattt
    20521 taaaaaataa tggtgtcaac tatgttcgac tgcgtttgtg gcaggatcct tataatagta
    20581 atggagaaag ttatggagca gggacaaatg atctggaaac gacgattgac cttgcaaaac
    20641 gagcaaaaaa tctgggactt aaggtgctgc tggattttca ctacagtgat ttttgggttg
    20701 atcctgggaa acagaatctt cctaaagcat ggcaaggtct gacctttgaa gaaatgaata
    20761 cggcattgta cgactataca aagaacgttc tgtcggaaat gaagcagctg gacgtttatc
    20821 ctgatatggt tcagatcggc aatgaactga atagcggaat gctctggcct tatggcaagt
    20881 cttggggaga aggcggggga gaatttgatc gattggctgc ttttttgaag tctggtatcc
    20941 aagctgttcg agacactcaa cctaaaacaa ctccagtaat gctccactta gctgatggtg
    21001 gcgatacagg agcatttact tggtggtttg atgaaatcac gagtcgaggt gtgtcatttg
    21061 atttaattgg agtgtcgtat tatccgtatt ggcacggttc aatgaatgaa cttcaaacca
    21121 atatgaatgc gatcagccaa cggtatggta aagaagtgat cgtagttgaa acagcttatg
    21181 ggcatacaac agcgaatgcc gatactatgc caaatgcttt tggtgaagcc gaagcagctg
    21241 cggggggata tgaaccaaca ccgacaggac aagcagagta tttgctggat cttgccgatc
    21301 ggattcaggc agtaccgaat aatcgcgggg cgggcttctt ctattgggaa cctctttggt
    21361 acaacggaaa tgtgagttgg gcaacacaag caggtatgaa ttatctcagt gttcaatcca
    21421 ctatgggaaa cgaatgggac aaccaagcga tgtttgattt tgctggaaac gccttgccaa
    21481 gcttgcgtgc gtttaaacaa gcaggggcgc aaataaatct agttaaaaat gcaagtttcg
    21541 aaagcgatgg accaacgacc agtccttcca attggcaaac ctggtttcaa caaggaaccg
    21601 ctaagggtac gatcaaaacg gaagcgggca ctttttttgg ggactacaaa ctaacttttt
    21661 ggcaggatca agcatacgag gcatcagctt atcaaaatgt tagtggcata agcaaaggaa
    21721 cttacacatt gagtgcctat gtaatgtctg ggggagagtt tgatactaat cagttatata
    21781 tcaaaaatta tggtggagcg gaactaaata ctgcggttgt taaaacaagt gaatggacaa
    21841 aaattacaat caaagacatt ccaattacaa caggtgtttg tgaaattggg gtttatagca
    21901 aggcttcagg aaatgcttgg acgaacgttg attttgtttc attggtgaaa caatagactg
    21961 cggatcgttt cgattcaaga tcggaggttc ccttcagcca catcgttagc gtagaaggca
    22021 ggaatcgaac ctgcacctcg cctttgccaa aaataaccag aaacgatttc cagtcatttt
    22081 taccaaaacg agtaaagcac cagacttcat ctacactttg tattatgcta aagagagttc
    22141 aacgatttcg taagaagcaa tgaaaaaatt gcaaaaagtt gtgcagataa cataaagttt
    22201 gtttaagaaa gggtaacctt agaattccgt cgtgaatatg atttgagcac ttagatggat
    22261 agaaaaaagt tctcggaaaa cagttcgttt ttcgagaact tctttttatg ataagaatga
    22321 aacaaattaa cttatattat agtctctttt ggtagggaac catcttattt ttaatgaaaa
    22381 tcaagcattt tagctattga agaatgttga tttataggga ttccccctcc atattatgtt
    22441 ggctctttat tcccactctg ttttcaacgt aaagtgtata aaacagaatg cgatactatc
    22501 aagggagtta aaacaatata cagaacagta aaacactata aataaaaata aaagtgcaat
    22561 aaaagtgcaa tttttgcacc taatcatact ctgtttatat atgaaaacta gattctgaat
    22621 ccaatatttg gttaacgaga ggcgttcgaa tttgaacgtc ttttatatat tactttcaaa
    22681 taaatatgag gtagaatact atctattttc tttttaaccc aattgttata atggagagtg
    22741 taaaatattt tgtgtaaata gaaaaaagga agtcccttct gtagaataga gttaccacaa
    22801 cacattcaca gaagagagga cttccctatg aacgatttta ctacagaaat tctaaagact
    22861 ctagcgaaca aaggcgattt gaatgaatta ttccgtgtcc atttggagaa agcagtcaat
    22921 acgcttctca aaacggagtt aacggctttc ctagattacg aaaagtatga tcgcattggt
    22981 tttaacacgg gtaattctcg taacggctcc tatgaccgta cggtcaagac cgagtatggg
    23041 gaacttcatc tccagattcc gcgcgaccgc aatggtgagt tcaagcaaca gactgttcct
    23101 gcttatagac ggacgaatga cacgttagag gagaccgtca ttcacctctt ccgaaaaggt
    23161 attaccatgt cggaaatcgc agacttgatt gagaaaatgt atgggcatta ctacacgccc
    23221 caaaccatgt ccaatataac aaaatcattt acagaagagg taacggcgtt taatgggcgg
    23281 gagcttcatg accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg
    23341 aaaaccgtcg ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag
    23401 gaagtattga gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta
    23461 ttggaccttc aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag
    23521 gggatggtag gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta
    23581 cacgtttccc gtaatatcag tcataaagtg cgtgtcgatg atcgtaagga agtctgtgat
    23641 gattttaaaa tggtgtatca agcctcatct aaagaggtgg cgttggaagc acgtggtact
    23701 tttgcggaaa aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat
    23761 cacttgctca ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac
    23821 ttgattgaat cctttaataa acaaatcaag aaatacagcc accgcaagga acagttccaa
    23881 aatgaagagt cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc
    23941 ctaggtcgca gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctaagc
    24001 caactgattg agaattagaa acaatctgca gtagggaaga atcatttaca caaaattatt
    24061 gacgctccct tataatgaga acttttacta acataattta tgataaactt actatataat
    24121 catattaatt aaatagttag gagatctgct tatgtttata tctactcttc aaattcgaaa
    24181 ttttagaaat tatgaatccg aaatctttcg attcgataat gagacaaata ctattatagg
    24241 tgagaatgat tcagggaaaa ccaatgctct tactgcattg agaatcttat tagatgatag
    24301 ttattactat agtagtaaaa cactaaaaga atcagatttt tttcatggaa ttcaaaatgg
    24361 gtggcaaggg cattggatta ttatctctgc gacatttgaa ggaatcagcg aagaagaatt
    24421 tgacaatgag atttgtgctt ccatttcttt aaattctgaa agtcaaacaa tcttagaaga
    24481 attgatttca aatgcagata aaggtgtagg tagtatttct ctatttatta gacctaacaa
    24541 agctattaga aagcaattat ttgatatttc cactgaatta gactctcatc aatttaacga
    24601 atttagaaat tctattcgtc tatccgacta tgaattttac tacacatcta aatcaaatct
    24661 aaatttctgt atcaatgaaa attatgataa attggtagga agactcaatg acaatatagc
    24721 tagtaatcct gaagaagacg atgaagctct gttgggctca agaattgaca tgtcagatat
    24781 tttcaagcat atttcggttg tttatgttga cgctcttcga gatgttctta gagaaatgaa
    24841 aaacaatcga aacccagtca aaagaatcat ggaaacaatt gaatcaaaaa tatcatcaga
    24901 taatgttgag tcattaaaaa caattataca gcaattaaat gagactatta ccagtgttcc
    24961 tgaaattagg aaaattggcg aaaatattaa tcggcaacta aatagcataa ttggttcagt
    25021 gtattctcct aatcttcttc tttcctcaac tatgtcagat gatatgggtt ctttagcaaa
    25081 atttctttca atgaagccag aacaaaatat cgacttagac ttactagggt taggacatct
    25141 aaatatgatt tatttagctt tgaaaatagt cgaatttgaa gcgtgtcgtt caagagaact
    25201 acttaatatt atgttgattg aagaaccaga agctcatatt catcatcata ttcaaaaaac
    25261 tctgttcgaa ggattaaatc ttcaaaaaaa ctatactcag attctcatga ccacacattc
    25321 tgtacattta gctgaagtgt ctgaaatatc tcggatgaat gtattaaaat cgtttaatgg
    25381 caaatctatc gttatgaatc cagcaaataa tctgaactca tttgctaaag aaaagttaaa
    25441 aaagaacaat ctaaatctaa taaacgcagt agagagatat ttagatgtta aaagaaatgg
    25501 attgcttttt tctaaaggtg ttatcttagt tgaaggagat gctgaagaaa tacttattcc
    25561 tcaaatagta aaaaaattat ttggtataaa cttagacgaa cttggaatag gtttgattaa
    25621 tgttggtagt acttcgtttg aatatatcgc tagcttgttt gacgatgata gaatacagag
    25681 aaagtgtgct attgtaactg atttagatga acaggcaatc ccatcagaac atcgattgta
    25741 taaaagcact gcacaagaga aaggtcaagc aagaaaagaa aaattatcct ccctttttaa
    25801 tgaaaacaat tgggttaatt gtttttatgc taatacaaca tttgaaattg agtttttaga
    25861 tgtgaataaa gaaaatattc taacttatgt cagtccaatt atagatgacg cattcaaaga
    25921 taaaaaaaca ataactgctt ataaagaaga aatacaaagt gaacaatatt tagaaagaaa
    25981 tttagcgatg ttaaaactag ccaatcatgt tggtaagggg tggtttgcaa ttgagttagg
    26041 tgaaaaaatt gactttttaa tcaaaattcc taagtatatt cttaatgcta ttgcttttgc
    26101 atgtcaggaa atagtagatc tctcaattta taaaaaaatt ctactccata caatcgactt
    26161 gtacgaggaa actacagaat tgtcagcttt atcagctagt ttaaaaacaa gtgatacagc
    26221 tgatttgaat atagcaataa tttctttatt agagtacttt ggtaaagatg aggatgttgt
    26281 gtatctaatc aatcagatag aaaattacac aaaaatactt tatgctgagg tttcaaaatg
    26341 acaaatatct tcgaaaaaat cgtaaatgat ttaaatttag aacaaaaaga tgttgtggaa
    26401 agtgaagata acctttatgt aaatgcatgt cctgggagtg ggaaaacaag ggtacttaca
    26461 aggaaaattg cttaccaagc aattaaacac gatggatcac taaaaagaat aattgctatc
    26521 acatacacta atagagctgc tgaagaaatt aaggaacgcc taagtttact tagtatagat
    26581 gatgatatta atatttgggt ggggacaata catcagtttt gtcttgaatt tattatttat
    26641 ccttttaaaa tgaatctctc aagagtatca aaaggattta caattattga tgattatact
    26701 caaagagctt acgtcagcca aattaataat atgctgaatt taaatctcaa aacgtatgaa
    26761 ctgaacaaaa ttgatttgtc actttctcat gattttaaaa ttaatgaagt tcagtatact
    26821 caagtttctg aaatgtatca cactatgttg gaaaaaaata aagaaattga ttttgatatg
    26881 attttactag tagcgtataa aattttaact gtaaacagta acgttgcacg taatattgct
    26941 agtaatataa gggcgattta tgtggatgaa tttcaagata ctagagaatt acaatacaat
    27001 gtaatagcta aattacttca aaacaaccct caaattcagt caatgtttgt tggagatatt
    27061 gaccaagcaa tttatggatc attagatggt atagctaaat cagttgaaga actagaaatg
    27121 ctaactagtc acacttttca atcaaagacg ctacatggct gttacaggtc taatcagaga
    27181 ttagtagact tctactctaa ttttcagagt tgtccttacg agattgaaag cagagggaat
    27241 aataaaaacg accgtggttt tataagttat gattgcaaag ttactaagga ggatttaccc
    27301 aacataataa aaaatataat aaaggaaaag attgaaagcg gcattcatga aaaagatatt
    27361 tgtgtacttg ctcctcaaca ctatcctctg ttttcactgg gtgagtattt aaagaaagaa
    27421 ttaccgtatt gcaattttga tgctataaat atatcaccaa ttaaggtaaa taatcatagt
    27481 ttatttttca agttagcaat tttgtacttt actgaatcag gtgaaaaggt tcgaagaaga
    27541 aagctaattg caaacgatat tttaattata ttaaaaaatg aatttggaat agaagttaaa
    27601 gattatttta ttgacttaga tttattacag ctaataaaca gtgtgaaaag aatgtatagt
    27661 ggagacgaca acggtgttga tttatacaaa tttgttacca aacatatatt tgttaatctt
    27721 aatattgacg agaaaaatta cccgtctatt tttaagcact atctattttt tattaacgaa
    27781 gtagaagatc ggataaaaaa taattcatta tctagtagtg tatctagttt taagaagatg
    27841 tttaaatctc gagatggtat cacacttact acagcacata aagttaaagg aaatgagtat
    27901 gatactgtta tcgctattaa tcttctaaac gggaaaattc ctcattggaa tgaaattttt
    27961 aatgtttctg ataaaggaat tagttcagcg agaaaactac tatacgtaat ttgttcaaga
    28021 gcaaaaaata atttatttct tttctcagag gtgggttatt ttacgagtaa aggagacttg
    28081 ctttatccca ctcaattatt agataaaata gaatttgatt ataatattta aaatacctag
    28141 gtagtcgttg ttttaaatat agtaggaatc ggacgctcag tacggagcgt ctattttttt
    28201 acccaatttt gaaaggaagt gaacttatga aaataaaaaa tcaagaatca aaaggtcgct
    28261 ctcccccctt taaaaccatc acacaaataa gattcattca aacataaaaa cagaaaggat
    28321 aggtaaaaat atggaactta aatatgtcat tcccaatatg gaaaaaacat ttggacaatt
    28381 agaatatgct ggtgaaggga acattgaaca acgtcgaata aatggcagac caaccattct
    28441 ctctcgtagt tacaatttat actcaacgat tcaacgagca gatgatattg ttgtcattct
    28501 tccctccgaa gctggcgaga aacattttga atccgacgaa aaagtcaaac tcatcaaccc
    28561 acatattacc gcagaaggtt ataaaattgg caatcgagga tttaccaatt acattatgca
    28621 cgcagacgat atggtgaaag cataagaaag gaaaggtgaa aaaatatgag attagcagaa
    28681 ggtattgtga ttgaaaaaga ccagacattt ggcacattga aattttccgc cttgcgtcgt
    28741 gaggttcgta tccagaatga agatggtacg gtttccgaag aaattaaaga acgtacctat
    28801 gacttaaaat caaaaggaca aggacgcatg atacaagtca gtatccccgc ttctgttcct
    28861 ttgaaagaat ttgactacaa cgcagaagtg gaattaatca atcctgtggc agataccgta
    28921 gcgacggcta cttttcgagg ggcagacgtt gattggtacg tgaaagcaga cgatcttgta
    28981 ttaaaaggac agacaaaacc tcatcagaaa tataaaaaag aagcgtccga aagtgaaaaa
    29041 taggtgttgc ttatgacaaa cacaccattg attttgaaag aaattccgaa ggatgaagcc
    29101 atttctttta ttcggcaata ccattattca aagatactcc ctagattgtg taaatacttt
    29161 ctagggattt tttcagaaga aaaattatta ggcgttgtgg aattaggctg gggtacgcaa
    29221 cccctacaaa ccatacgcaa actctttccc gacagttccc ttcaaaccac agattattta
    29281 gaaattggaa agatgtgttt tcttccagaa atgaatcaaa ccaattattt tggcagtcaa
    29341 gctctttcag ctctaattaa gtggttgaaa gaacatacgg actgtcattt tttatataca
    29401 ctggcagatg gaattgaggg aaaatgtggc tatgtctatc aagcgtccaa cttcttttac
    29461 tgcgggtatt tcaagacaag tgtctatcgt gataagcagt catgggaaaa gatacaccct
    29521 cgaagtgctc gccttttatt agaggaaaat gctcgctttg aacaggtaga gaaaaagcat
    29581 tggctttccc aggcattttg cgaatacaaa gggattgaga aaatcaatgg acgcatgttt
    29641 cgctatctct atccgttgac aaaagaagcg aaaaaactgt taggacacac tctctatcga
    29701 cgtcattatt atccgaaaga aaaaaattta cgctttgaaa agcgaatcgc ttatcagaaa
    29761 tatgaagcca tttctcaacc aacttttgat aaacaggctc gtatctacaa tacacaatta
    29821 ttttgatagg tggaaaggag gatttttatg ccattgcttc atgacagagg agagcgaatc
    29881 aaagccaacg ataagcactt ggtctatcat tttgtactaa tttcattgct ggtagttttt
    29941 ctagtcgtcc tactcccttt tcattggaga gcgttacaat cggtgaactg gcaagcagtt
    30001 cagttagata catttttcta tcaacttcat acgccatact tttggagcag tattctcact
    30061 gctctttttg tatgcggact tatcagctgg ctttattatc actatcgtat agattctata
    30121 aaacagttag agcaccgtca aaagttagct cgtatgattt tagaaaatca ttggtatgaa
    30181 gccgaagaaa ctcaatcaga gggattcttt aaagatttac ccagcaagaa aaccaaacaa
    30241 cgaatccgcc attttcctaa aatatattat cgaatgaaag atggactaat ccatattcat
    30301 gtagaaatca ccttagggaa atatcaagat cagcttttgc acctagaaaa caaactagaa
    30361 acaggattgt attgtgaatt agtggaaaaa acgttgaaag attcttatgt agagtatgtc
    30421 ctactttatg atactattgg caatcgaatt ggcattgaag acgtggtagc tcaagatgga
    30481 cgtgtcaaat tgatggaatc ggtctattgg gagttcgatt ctctccctca catgttaatt
    30541 gctgggggca ctggtagtgg aaaatcctat tttattctca cattgattga atctctcctg
    30601 catacaaatg caaagcttta tattcttgac cctaaaaatg ctgatttggc agacttaggg
    30661 gcggtcatga ataatgtcta ttaccgtaaa gaagatatgt tggcatgtat caatcaattt
    30721 tacgaagata tgattgatcg cagtgaaacc atgaaacaac accctaacta taaaacagga
    30781 gaaaattatg cttatcttgg tttgtctgcc aattttctta tttttgatga atatgtggca
    30841 atgatggata tgctaggtcg tgaaagtacc accgttattc ataaactcaa acaaattgta
    30901 atgttaggac gacaagcagg cttcttttta attcttgcct gtcaacgtcc agacgcaaaa
    30961 tattttagtg atgggattcg ggacaacttt aacttccgtg tggctttggg tcgtatgtct
    31021 gaaatgggct ttggtatgat gttcggaagc gatacacaaa aacaattttt cttaaaaccc
    31081 atcaagggaa gaggttacgt ggatacagga aaaagtgtga ttagtgagtt ttatacaccc
    31141 cttgtaccaa aaaggtacga tttcttagga gaaataggaa aagtcattca aaaaaagcaa
    31201 tctgagcctg taccgcacga agtgaaaggt acaggttcag acttgctttg attggtgtgg
    31261 cgttagccac gccaatcctt tagccccctc tatctaacag gggggcacaa atacaactga
    31321 aaacaggcaa ttctcatcaa ccaatgttga tggtacaaag gattgcggaa aatatgaaag
    31381 gtgtaaactc aagggtcata gtttacatct tttttagatt ggaggtttgc gaatggaaaa
    31441 cgaaaaaatt tggattaagg aattgaaaga aaagcgactg gcttatggcg tttctcaaaa
    31501 taaactggcg gttgcttctc atattaccag accttaccta tcggatattg aaacaggcaa
    31561 ggcagtccct actcaacagg tgaaagaaga tttactgaac gctttggaac gcttcaatcc
    31621 cgataaccca ttggagatgt tattcgacta tgtacggatt cgattcccta ccaatgatgt
    31681 ggctcaaatt atcggtgagg ttttacgttt gaatatggat tatatgctac acgaagactt
    31741 tggctattat tcttatcccg aacactatcg tttcggtgat attgttgtcc tagtctctca
    31801 tgatgtaagt aaaggtgttt tattagaact gaaaggaaaa ggctgtcggc aatttgaaaa
    31861 ctttctattg gctcaacatc ggagctggta tgatttcttt caagattgca tggaacataa
    31921 aggtattttc aaacgcttgg atttagctat caacgataaa acagggattt tgaatattcc
    31981 agatttggca aagaaatgta aacaggaaga atgcatttcc gtctttcgaa gctttaaaaa
    32041 ttatcgcagt ggtgaattgg ttcaccgtga tgaaaaacca tacatgggaa acacacttta
    32101 tattggctca ttaaaaagtg aagtctattt ctgtatatat gagaaagatt atgagcaata
    32161 cgtgaaaaat gatgttccct tagaagacgc agaagtaaag aatcggtttg aaattcggtt
    32221 aaaaaatgac cgagccaccc aagccatgaa agatttattg gcacatcaac aggcggaaaa
    32281 aacagccttt gaaattatca atcgctatat ccgctttgca gataaagacg acactaaacg
    32341 tcggagtgat tggaaaacta atgaacgttg ggaatggttt atcggtaaaa atcgtggagc
    32401 attacgccta accacacagc cagagcctta ttcttttgaa cgaacattga actggttaca
    32461 tcatcaagtc gcccctacat taaaaatcgc aagcatttta gatgttctca acggtacaac
    32521 cattatctcc accatgattc aggaagccaa actgacagaa aaacatgaga aattaattga
    32581 acaacaacat ctagccatgg aggatttgat tacatgagcc ttttaacaaa agaattaaag
    32641 aagttagggt ttcagtgtgg cattgagttt caagcgtata tacaaaatac agggaaatat
    32701 acgtctctca ttattgaggg aaaacggcaa gctggtgata ccatctatac ctatgatttt
    32761 tacaaggtga gattttataa taattatacc aatcgagtaa ccgtctatgg agagcattta
    32821 accccttttc aattattaag gcgtgtaaaa agctatatct attatcgaga aaaatatctt
    32881 aaggaaagga ggacaatcac atgaattttg ggcagaattt gtacaactgg ttcttggata
    32941 atgcacagtc attggtattg ttagcgattg ttgtcattgg actgttttta ggttttaaac
    33001 gagaattttc aaaactcatt ggcttcttga ttattgctct aatcgccgtc ggacttgtgt
    33061 tcaatgcagc tggcgtgaaa gatgtgttat taaacttatt taaccgaatc attggtgctt
    33121 aacagatatt tttcatgata aaataggaag tatatatttt gttatgaaag gatttatgaa
    33181 tatgtttaca aatgatcaat ttaaatctct tttaattaaa acattagaaa gaaagagctc
    33241 ttcaaattat tatgaaggag gtaacgagat tgtttctaaa atgaaaatat cagaggtaac
    33301 tttagatgag actgacgatt ttacttatta taaaggatat aaaaagggct ggaatacgct
    33361 ttgcacatat cttaaaataa gagttccttt tgatgattta gacttttttg aaagtcataa
    33421 agatttgatt acccagacag caagctccat atatgataaa caaggtgata atgtattagt
    33481 ggatactatt ttagtaccac ttccagagaa ttacgaagta attaacttta gtcagttgaa
    33541 aattagtgat gtggtttcac aagctattga agacgctgag tcttttatga gtaatggtga
    33601 atatcaacgt gcctttgaca gagttcacac tgcttttcat ggatatttaa ttgaaatact
    33661 aaaaaaatat gaaattacag ttcctagaga cgaaaatcta tctaaattat atagtaggat
    33721 acaacaatta attgaaaaag aaattcaacc tactgaactt gctgatatag tgaagacaac
    33781 gataaggtca tcaaatggaa tgattagctc tcttaatgaa gcaagaaata ggcattcact
    33841 tgcacaccct aatacaaata ttattggaaa gagagaagca aaactaataa taggtatttc
    33901 aagtacagtt acagattata ttagcggata ccttgataag tagcgtcatt aactgattgc
    33961 tacttttttt atgactattt ttataaaaag gatgtgataa gaattggaaa tgaaagtcta
    34021 tgtggcaaat ttaggacggt ataatgaagg tgaattagtc ggagcatggt ttactccacc
    34081 aattgatgag gaagaaatgg cagaaagaat cggtttaaat gaggactacg aagaatatgc
    34141 aatacatgat tttgagcttc cttttgatgt ggacgaatac acgccgatta gtgaaatcaa
    34201 ccgtctatgt gaagccattc aagaaatcga aggtacacct atttataatg aactcaaaga
    34261 aattcaaggc atgtggttta gtagcttgga agaattactg gaaaacaaag aggatattca
    34321 ttgttatagt gattgtgatt cgatggaaga tgtcgcccgt tattacgtgg aagaaacagg
    34381 acagcttggt gaagtccctt ccaatttaca aaattatatt gactaccaat ctttgggacg
    34441 tgatatggaa atagagggta attacctagt tacctctcat ggggtatttg aatattgtca
    34501 gtaagtttaa caatccgtgt tataattaaa ttaataaata gcacggagag gtataaatta
    34561 tggtaaatgt atttgaaaat cttaaactat taaaactaga catgaaagat aaaggctggg
    34621 tgatagattc cttttacttt cgttacaaac agcaaaatta tattgtactt gttaagctat
    34681 ttgaaaaaga ggaaaaagtt ccagaatatg ctctgttaaa actagaattt ttaaaggaaa
    34741 acgatttttc agatatgcta gctgtctatg ctaattcggt aaaattatat acagacacga
    34801 aaacaataag agaatatttt ggtatcgaat acagtagtaa cttgggagat gttttatttc
    34861 aatttagcca aactcttgct cgatttattc cgacagaagt tagtgagaag aaaaatgaag
    34921 accaaaaaga agccatgtgt ttctcattaa gtcaaagtga ttcagaagat cccagaaaaa
    34981 aatattgttt ttcagttaga agaaatcctt taaagggaaa tggtgagctg ggaaaacgaa
    35041 gtccttttaa tgacaataaa acgagattgt atagacccag cttatatgaa cgattagggt
    35101 cagataccaa tttaagtttc agatattcaa tgaatccaga tgatgaagaa actgatgaag
    35161 gtattattgc aaaatggaca aaaaataaaa ttgaatagtt aaagcattga aagagcaatc
    35221 acctattttg attgttcttt ttctattttt acggaaagga cgtgaagaca tgaaaaaaat
    35281 aagaagttac accagtattt ggtcagtaga aaaagtgatt tatgcgatta atgatttaca
    35341 actccctttc ccaattacct ttacgcaaat gacatggttt atcgtttctt tgtttgtcgt
    35401 catactcttt ggcagtttac caccactttc tctgattgac ggagcactac tcaaatactt
    35461 agggattccc attgttctca ctgggttcat gtgccaaaag acctttgacg gaaagaaacc
    35521 ctatggattt ctaaagtcta tcattaccta tgctctacgc cctaaagtaa cctacgctgg
    35581 caaacaaata cgattaaaaa aagaaacaat gaatgaatcg attacgattg taaggagtga
    35641 acaactagat ggcatatccg attaaatata tggaagataa tctcgtattt aatcatgatg
    35701 gcgaagtatt cgcttattat gaattaattc cctataatta cagtttttta agttccgatg
    35761 aaaaaattca agttcatgat aatttctgtc aattgattgc ccaaaatcgt gatggcaaga
    35821 ttcatgctct gcaactagca accgaatcaa gtattcgagc cacacaggaa caatcgaaaa
    35881 aagaaatcac tggacgatta aaagagatag cttacgaaaa aatagatgaa cagaccgaag
    35941 cgttaatttc tatgattggt gacaatcaag tagactatcg cttttttatt ggttttaaac
    36001 tctacctcaa tgaacaggaa gtatccgtaa aaaatgttgg gaaagaaatt caaaaagcat
    36061 ttgcggaatt ctttcatggt gtgaatcaca aattaatggg cgattttgtt tctatgccta
    36121 tggcggaaat cgaacgcttt tctaagatgg aacagttgtt agaaagtaaa ttatccagac
    36181 gctttcagat tcgtaaattg gagaaagatg attttggtta tattattgaa catctttacg
    36241 ggcaaacagg tgtccctttt gatgactacg actaccattt accgaaacga tacgaagata
    36301 aaaaagtgtt ggtaaagaaa tacgatattc tcaaacccac tcgctgttta atcgaagaaa
    36361 atcaacgcta tttacggatt gaacaggaag ataccactac ttatgtggcg tattttacaa
    36421 ttaatagtat tgttggtgaa ttggatttcc ctaatagtga aattttttat taccagcaac
    36481 agcagtttac tttccccgtt gataccagta tgaatgttga aattgtcgcc aataaaaaag
    36541 ccttatctac cgttcgcaat aaaaagaaag aactgaaaga tttagatagc cacgcatggg
    36601 aaagcgacaa cgaaaccagt actaatgtgg tggacgcttt agaatctgtc gatgaattag
    36661 aaagtaattt agaccaaacc aaagaatcta tgtacaaact aagctatgtg gtacgggtat
    36721 cagcaaagaa tttggacgag ctgaaacaac gttgtgatga agtaaaagat ttttatgatg
    36781 atctgtctat caaattagta cgaccttttg gcgatatgct aggcttgcat aatgaattta
    36841 ttccctcaag caagcgatat atcaatgact atattcaata tgtcacgtcg gattttttgg
    36901 ctggtcttgg tttcggtgct acacaaatga ttggtgaaaa ttctggtatt tacgtcggtt
    36961 acaatctcga tacaggcaga aatatttatc tccaacctag ccttgcaagt caaggaatta
    37021 aaggctctgt aacaaatgca ttagcgtccg cttttattgg ttcattaggt ggcggaaaat
    37081 cattctctaa caatatgctg gtctattatt cagtactctt tggcggacag gcaaccattg
    37141 ttgacccaaa agctgaacgt ggtgaatgga aagaaacctt acctgatatt gcccatgaaa
    37201 tcaatattgt taatctcacg agtgagaacg acaataaagg gttacttgac ccttatgtga
    37261 ttttaaaact cccgaaagat tcagaaagcc ttgccattga tattctcacg ttcttaacag
    37321 gtatttccag ccgagatagc gagaagtttc ctgtcctgcg taaagctatt cggagtgtaa
    37381 ctcaaagcaa acaacgaggg ttgttattgg tgattgatga attgcgaaaa gaagatacgc
    37441 ccatcagcaa tagtattgct gaccatatcg aaagctttgt cgattatgat tttgcccatc
    37501 tgttattcag tgatggcagt gtcacccaat ccattagctt ggaaaaacaa ctcaatatta
    37561 ttcaagtggc agatttggtc ttacccgatt ccaatagctc ttttgaagaa tacactacca
    37621 tggaattatt aagtgtggca atgctgattg tcattagtac ctttgcttta gactttatcc
    37681 actctgaccg ttccattttt aaaattgttg accttgatga ggcttggagt tttttacaag
    37741 tggcacaagg aaaagctcta tctatgcgat tggttcgagc aggacgtgcc atgaatgcag
    37801 gggtctattt cgttactcaa aacgcggacg atttactaga tgaaaagatg aagaataata
    37861 ttggcttgaa atttgccttt cgttccactg acctagtgga aatcaaaaag accttagaat
    37921 tttttggtgt ggacaaggaa gatgaaaaca atcagaaacg cttacgtgaa ttagaaaatg
    37981 gacaatgttt agtttcagac atctatggtc gtgtaggggt tatgcagttt cacccgattt
    38041 ttgaagagtt gcttcatgcc tttgatacca gaccgcctgt gcgaaatgag gtggaagaat
    38101 gacacaaaaa caaaagaaat ttcttcacta tagttggata acgctccttt gtgtgggcgt
    38161 tattttactt ttattaggca cgctgggcaa tgcagtacaa gcgacaggct tagttgatga
    38221 aaccattgat acaagcaatg aatactccaa gtatggtttg aatcaatatc agcttgatta
    38281 ttatgtggac aatagctggg gctggcttcc atggaattgg agtgatggca taggacaatc
    38341 ggtgatgtat ggattgtatg ccattactaa ttttatttgg acaatttctc tttatctatc
    38401 gaacgccaca ggttatttgg tgaaagaagc ctatgcctta gatttcatta gtcaaacatc
    38461 ccaagcaatt ggcgaaaaca tgcagacgat tgcaggcatt acaccgaatg gcttttccag
    38521 ttcagggttt tatgtcggtt tcctccttct ttttattctc attgttggaa tctatgttgc
    38581 ctatacagga ttggttaagc gagaaacgac gaaagtgata cgagctgttt taaactttgt
    38641 cgtggtgttt attctatcgg cttcctttat tgcgtatgca cccgattata ttgcgaaaat
    38701 caatgatttt tccaaagatg tcagtaccgc cagtctgaat attgggacaa aaattgttct
    38761 tcctaacacg gatagtcaag ggaaagacag tgtggacatg atacgtgaca gcttattctc
    38821 tgttcaagta aagcaaccat ggctattgct tcaatatggc tccactgatg tagaagcgtt
    38881 aggggaagaa cgtgtggaaa gtttattgtc taccagtcca gatacaaaca atggtgaaga
    38941 tagagaaaat gtagtgattg aagaaattga agatcatgac aatacctatt taaccctacc
    39001 gaagacgatt tccagattag gaacagtctt ctttttgttt gtattcaata ttggtatctc
    39061 gatttttgtt ttccttctaa caggaatcat gattttttcg caagtgctat ttattatttt
    39121 cgctatgttt ttgccaatca gtttcctatt aagtatgttg ccgagttttg atggcatggg
    39181 caggcatgcc attaccaagt tatttaatgt gattatgaca agggctggta ttaccttgat
    39241 tattaccgtt gcctttagta tttccactat gctttattcg ctgtcagcaa gttctccctt
    39301 tttcatgatt atgtttttac aaattgtgac ctttgcaggg atttatttta aactgggcga
    39361 tttaatgagc ctattcactt tacaaagcaa cgactctcaa aacatgggca gacaagtgtt
    39421 tcgcagacct cgtcaaatga tgaatcgaca aaccagaaga tttcaacgca gtatgagtaa
    39481 gatttttcgt ggaaaagcag ggtcaacgaa gcaaaagaca atgacaacaa agcagaatca
    39541 aaccaataaa gctaaccata ctcgtcaaaa tgaacggact acaccaaaga aagaaacagc
    39601 actttccaat gtgaaaaagc aacaaggcgg tatcgggcaa cgattaggtg aaaagacagc
    39661 ccatgtattg gacacgaaaa atcgtctagt caataaaaga aagcaggcaa aacaacagat
    39721 acaggcgaca ccaacccatg tcaaatatgc ccttcatcaa ggaaaagaga atgtcactca
    39781 taatgtaaca gactttaaac aatctatggc agataccaaa gctcaacgca aacaacagcg
    39841 tatgaaccag caggagcaaa aacgaaagaa cattgctcaa aagcgtttgg agctggataa
    39901 agcaaaagaa aaaagacgaa aagaaaaccc atcatctcct gttcagaata accagcagca
    39961 tgttcgcaag cgtcagcaaa ccgtcacgcc tgttttgtca caaaacacta aacgtgaaaa
    40021 tcatcagcga ccattgaaaa aagagcaaaa acaaaagaca ccaccgaagc aattcaatca
    40081 gaggaagcta aataaaagaa cggataagcg aggacaacaa acatgaagct aaaaataaca
    40141 gtgatttgta gtgtggtctt ctctctcttc ttttttctct tattattcct agtcattttc
    40201 tttgcagatg atacggacag tggcgaaaac aacaaggatt cttctattcc acaaggcggt
    40261 gtgaccgttt caccagaagt gttggcacac cgtccgttga ttgaaaaata tggtaaggaa
    40321 tatgggattg aagactatgt ttcttatatc ttagccatta tgcaagtgga atcgggcggt
    40381 acagcagaag atgttatgca aagttccgaa tctttgggtt taccgcccaa cagtttgagt
    40441 accgaagagt ccatcaaaca aggggttaaa tatttcagtg aattattaac cagtgcagaa
    40501 caacaaggag tggatataga ttctgttatc caatcttata attatggcgg tggcttttta
    40561 aattatgtga ggagtcatgg gaaaaaatat acctatgaat tagccgaaca gttttctaaa
    40621 gagaagtcgg gtggtcaaaa agcagactat cccaatccta tagccatacc tgtgaatggt
    40681 ggttggcgat ataactatgg aaatcagttt tatgttcagt tggtttcaca atacctaacc
    40741 gacacttccc caacagaatt tgatgatgaa accgtgcaag tcattatgga cgaagccttg
    40801 aaatacgaag ggtttcccta tgtctttgga ggggcgtcgc ctaccacctc ttttgattgt
    40861 agtggcttaa tacaatgggt gtatgacaaa gctggcattt ctctccctcg tgtcgcccaa
    40921 gatcagtatg acgctacgca agaaatctct atggaagaag cccaagcagg cgaccttatc
    40981 ttctttcatt caacctataa tgcaggcaca tatgtgactc atgtggcgat atatttagag
    41041 ggaaatcgct tttatcatgc aggcgaccca atcggttatg gcgacttaag cagtcgttat
    41101 tggcaagacc atctgattgg ggctagacgt gttattcata actaagaaag gaatgaatca
    41161 aatgattata aaaatcgagc gaaaagaaaa gaaggagaaa ccaaagaaat ctaaaaaacc
    41221 gagcaagcaa agaaaagttc cgatgattaa agttggcacg cataagaaat taacctttgt
    41281 tttatgggtt ttattgattg gtagtgtcgg atttggaatc tataaaaatt ttactgccat
    41341 tgacactcat actgtccgtg aaacagaaat cattaaacag cagattgtcg ataccaacca
    41401 agtagaaagt tttgtgaaat cctttgcgaa agactacttt tcgtggcaac aatcccaaga
    41461 agccattgat aaacgaaatg aaaaattgac ccattatttg acggaagaat tgcaggtatt
    41521 aaatgaagaa atgattcgta aggatattcc tactagttct agtgtgaacg atatacaggt
    41581 gtggcaggtt tctcaagtga atgagaacac ctttgaggta ctgttttctg ttgaacaagt
    41641 tattaccgaa gacaaagaca aggaaactat atcgtctagc tttcatgtgg ttgtccatat
    41701 agatgaatca gataatatgg ttattatcaa aaatccgaca atgagtaaga aacctcaaaa
    41761 atcggactac caacccaaac agctagaaag tgaccataca gtagatacag aaacgatgga
    41821 cgaaatcact agctttttag aaacgttctt ccagctctat cctaccgcca cagaaaaaga
    41881 attgacttac tatgtaagta atcatgtgtt acctatgatt aacaaggaat atgttttcga
    41941 ggaattggtg aatcctatct ttaccagaaa agataaccaa gtaatcgtga atgtcgctgt
    42001 gaaatattta gatcaagaaa caaaggcaac ccaaatctcg caatttgagt tgatattaga
    42061 aaaacaagat aattggaaga ttgtaaaata acaaatattg gtacatgatt acagatactt
    42121 tgtaatcatg tactcttttt gataaaaaat tggagattcc tttacaaata tgctcttacg
    42181 tgctattatt taagtgacta tttaaaagga gttaataaat atgcggcaag gtattcttaa
    42241 ataaactgtc aatttgatag cgggaacaaa taattagatg tcctttttta ggagggctta
    42301 gttttttgta cccagtttaa gaataccttt atcatgtgat tctaaagtat ccagagaata
    42361 tctgtatgct ttgtatacct atggttatgc ataaaaatcc cagtgataaa agtat
//
LOCUS       DAZUMM010000016        39624 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000016 DAZUMM010000000
VERSION     DAZUMM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.016"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..569)
                     /gene="frr"
                     /locus_tag="ACG8E6_000778"
     CDS             complement(12..569)
                     /gene="frr"
                     /locus_tag="ACG8E6_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFS5061221.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(572..1294)
                     /gene="pyrH"
                     /locus_tag="ACG8E6_000779"
     CDS             complement(572..1294)
                     /gene="pyrH"
                     /locus_tag="ACG8E6_000779"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFS5061222.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1430..2311)
                     /gene="tsf"
                     /locus_tag="ACG8E6_000780"
     CDS             complement(1430..2311)
                     /gene="tsf"
                     /locus_tag="ACG8E6_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFS5061223.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2410..3192)
                     /gene="rpsB"
                     /locus_tag="ACG8E6_000781"
     CDS             complement(2410..3192)
                     /gene="rpsB"
                     /locus_tag="ACG8E6_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFS5061224.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3551..4030
                     /locus_tag="ACG8E6_000782"
     CDS             3551..4030
                     /locus_tag="ACG8E6_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS5061225.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4247..5338
                     /gene="phnW"
                     /locus_tag="ACG8E6_000783"
     CDS             4247..5338
                     /gene="phnW"
                     /locus_tag="ACG8E6_000783"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFS5061226.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5331..6116
                     /gene="phnX"
                     /locus_tag="ACG8E6_000784"
     CDS             5331..6116
                     /gene="phnX"
                     /locus_tag="ACG8E6_000784"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFS5061227.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6462..8153)
                     /gene="argS"
                     /locus_tag="ACG8E6_000785"
     CDS             complement(6462..8153)
                     /gene="argS"
                     /locus_tag="ACG8E6_000785"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFS5061228.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8575..9522)
                     /gene="arcC"
                     /locus_tag="ACG8E6_000786"
     CDS             complement(8575..9522)
                     /gene="arcC"
                     /locus_tag="ACG8E6_000786"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS5061229.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9637..10656)
                     /gene="argF"
                     /locus_tag="ACG8E6_000787"
     CDS             complement(9637..10656)
                     /gene="argF"
                     /locus_tag="ACG8E6_000787"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFS5061230.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10747..11976)
                     /gene="arcA"
                     /locus_tag="ACG8E6_000788"
     CDS             complement(10747..11976)
                     /gene="arcA"
                     /locus_tag="ACG8E6_000788"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFS5061231.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12437..13138
                     /locus_tag="ACG8E6_000789"
     CDS             12437..13138
                     /locus_tag="ACG8E6_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5061232.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(13710..14726)
                     /gene="tsaD"
                     /locus_tag="ACG8E6_000790"
     CDS             complement(13710..14726)
                     /gene="tsaD"
                     /locus_tag="ACG8E6_000790"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFS5061233.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(14723..15187)
                     /gene="rimI"
                     /locus_tag="ACG8E6_000791"
     CDS             complement(14723..15187)
                     /gene="rimI"
                     /locus_tag="ACG8E6_000791"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS5061234.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(15194..15736)
                     /gene="rimI"
                     /locus_tag="ACG8E6_000792"
     CDS             complement(15194..15736)
                     /gene="rimI"
                     /locus_tag="ACG8E6_000792"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS5061235.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(15720..16442)
                     /gene="tsaB"
                     /locus_tag="ACG8E6_000793"
     CDS             complement(15720..16442)
                     /gene="tsaB"
                     /locus_tag="ACG8E6_000793"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFS5061236.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(16633..17613)
                     /locus_tag="ACG8E6_000794"
     CDS             complement(16633..17613)
                     /locus_tag="ACG8E6_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5061237.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(17637..19121)
                     /gene="galT"
                     /locus_tag="ACG8E6_000795"
     CDS             complement(17637..19121)
                     /gene="galT"
                     /locus_tag="ACG8E6_000795"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFS5061238.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(19133..20122)
                     /gene="galE"
                     /locus_tag="ACG8E6_000796"
     CDS             complement(19133..20122)
                     /gene="galE"
                     /locus_tag="ACG8E6_000796"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFS5061239.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            20371..20538
                     /locus_tag="ACG8E6_000797"
     CDS             20371..20538
                     /locus_tag="ACG8E6_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFS5061240.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(20600..22411)
                     /locus_tag="ACG8E6_000798"
     CDS             complement(20600..22411)
                     /locus_tag="ACG8E6_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFS5061241.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(22408..22773)
                     /locus_tag="ACG8E6_000799"
     CDS             complement(22408..22773)
                     /locus_tag="ACG8E6_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFS5061242.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(22936..23331)
                     /locus_tag="ACG8E6_000800"
     CDS             complement(22936..23331)
                     /locus_tag="ACG8E6_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS5061243.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(23349..24311)
                     /locus_tag="ACG8E6_000801"
     CDS             complement(23349..24311)
                     /locus_tag="ACG8E6_000801"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFS5061244.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(24311..24523)
                     /locus_tag="ACG8E6_000802"
     CDS             complement(24311..24523)
                     /locus_tag="ACG8E6_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFS5061245.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(24544..25242)
                     /locus_tag="ACG8E6_000803"
     CDS             complement(24544..25242)
                     /locus_tag="ACG8E6_000803"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFS5061246.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(25262..25804)
                     /locus_tag="ACG8E6_000804"
     CDS             complement(25262..25804)
                     /locus_tag="ACG8E6_000804"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFS5061247.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(25936..26940)
                     /locus_tag="ACG8E6_000805"
     CDS             complement(25936..26940)
                     /locus_tag="ACG8E6_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS5061248.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(26937..27926)
                     /locus_tag="ACG8E6_000806"
     CDS             complement(26937..27926)
                     /locus_tag="ACG8E6_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS5061249.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(27923..28729)
                     /locus_tag="ACG8E6_000807"
     CDS             complement(27923..28729)
                     /locus_tag="ACG8E6_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5061250.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            28895..29851
                     /locus_tag="ACG8E6_000808"
     CDS             28895..29851
                     /locus_tag="ACG8E6_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5061251.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(29928..30446)
                     /locus_tag="ACG8E6_000809"
     CDS             complement(29928..30446)
                     /locus_tag="ACG8E6_000809"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS5061252.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(30534..30683)
                     /gene="rpmG"
                     /locus_tag="ACG8E6_000810"
     CDS             complement(30534..30683)
                     /gene="rpmG"
                     /locus_tag="ACG8E6_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS5061253.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            30911..31357
                     /locus_tag="ACG8E6_000811"
     CDS             30911..31357
                     /locus_tag="ACG8E6_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFS5061254.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(31552..33447)
                     /gene="asnB"
                     /locus_tag="ACG8E6_000812"
     CDS             complement(31552..33447)
                     /gene="asnB"
                     /locus_tag="ACG8E6_000812"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFS5061255.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(33772..34746)
                     /gene="bsh"
                     /locus_tag="ACG8E6_000813"
     CDS             complement(33772..34746)
                     /gene="bsh"
                     /locus_tag="ACG8E6_000813"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFS5061256.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(35082..35169)
                     /locus_tag="ACG8E6_000814"
     tRNA            complement(35082..35169)
                     /locus_tag="ACG8E6_000814"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35135..35137),aa:Ser,seq:cga)
     gene            complement(35314..35880)
                     /locus_tag="ACG8E6_000815"
     CDS             complement(35314..35880)
                     /locus_tag="ACG8E6_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5061257.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            36135..37466
                     /locus_tag="ACG8E6_000816"
     CDS             36135..37466
                     /locus_tag="ACG8E6_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFS5061258.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            37432..37782
                     /locus_tag="ACG8E6_000817"
     CDS             37432..37782
                     /locus_tag="ACG8E6_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061259.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(37874..38293)
                     /locus_tag="ACG8E6_000818"
     CDS             complement(37874..38293)
                     /locus_tag="ACG8E6_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFS5061260.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(38294..38638)
                     /locus_tag="ACG8E6_000819"
     CDS             complement(38294..38638)
                     /locus_tag="ACG8E6_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFS5061261.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            38988..39584
                     /locus_tag="ACG8E6_000820"
     CDS             38988..39584
                     /locus_tag="ACG8E6_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS5061262.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT        11492 a         8187 c         6888 g        13057 t
ORIGIN      
        1 ttatgatttt attaaacttc caataattct ttttctttat cagcagcgat cttatctact
       61 tctttaatgc tgttatctgt caatgtttgt acgtcttttt ctaatccgcg caaatcatct
      121 tcggtaatat caccattttt ttgttgtttt ttatattcat cgatcgcatc acgacggatg
      181 ttacgaatcg cgatttttgc attttctgct tcttttttca catctttagc taattctttt
      241 ctgcgttctt ctgtcagttg cgggataact aagcgaatga catttccatc attggttgga
      301 ctgattccga tatcacttgc cataatagct ttttcaacat cttgaagaat gctcttgtca
      361 aaaggtgtga tcattaaaac acgtgcttct ggaatttgga ttgatgccat ttggttcaat
      421 ggtgttggcg caccgtagta gttcacagta atacggtcca aaaggctagc gttcgcacgg
      481 cctgcgcgga tttgcccaag ttcacgttga agattttgtg ctgctttttt cattttttct
      541 tttgcttctg tcataattgc atctgccatt tttatttccc ccttacagtt gttccgatat
      601 tttcgcctaa gattgcacgg cgaatatttc cgtgttcatt taaattgaag acaagcagtg
      661 gaatatcatt atccatgctt agtgaacttg cagttgaatc catgacttgt aatcctttgg
      721 caataacttc cagatgagtc agttcttcaa attttactgc atttgcatct actttcggat
      781 cagcagaata aacaccgtct acattgtttt tagccatcag gataacatct gcgccgattt
      841 ctgccgctcg taatgctgct gttgtgtctg tcgagaagta cgggttgccg gtaccgcctg
      901 caaagataac gatccgtcct ttttctaaat gacgttctgc tttacggcga atatatggct
      961 ctgcgatttg acgcatttca atcgaagttt gcacgcgtgt cggtacaccc acgttttcca
     1021 acgtatcctg taaagctaag gcattcataa ctgtagctaa catacccatg taatcagctt
     1081 gagcacgttc catgcccatt tgtgctccga tttgtccgcg ccagatattt ccgccaccca
     1141 caacgattgc catttcaacg cccagctcgt gaacttcttt gatttcttgg atgatttctt
     1201 tgattactgg tggtttgatt ccaaatcctg catccccagc taaagcttct ccactcaatt
     1261 ttaagactac acgctgatat ttcggtgtga ccatgttcat tcctccaata ttttctattg
     1321 ccattttacc atacttagat gatttcgaca ctctaagact ggtttttctt gttttgctta
     1381 aaaaaaggga gcgcacaaac cgtgcgttcc cgtctgttat tcaacagaat tattttttca
     1441 cttggctcat aacttcttct acaaagttat cttcgcgttt ttcgatacct tcgccaactt
     1501 cgaaacgtac gaatgtttta acagttgcgc cttttgatgc aacgtatttt tcaacagtca
     1561 tatcaggatc tttaacgaat ggttggtcca ccaaagcgat ttctgctttg aatttcttca
     1621 agcgtccttc taccattttt tcaacgatat tcgctggttt gccttcgttc aatgcttgtt
     1681 ctgtaagaac agttttttca tgttctaatt cagcttctgg aatttgtgat tcgtttacat
     1741 aacgaggatt gatagctgct acgtgcatag ctacgtctct tgcaactgtt tcgtcagttg
     1801 ttccatctag aacagtcaat acggcaatac gtccgcccat atgcaagtat ccaccaaatg
     1861 cagcattgtc gtctttttct acaacttcaa agcgacggaa gctgattttt tctccgataa
     1921 cttgagttgc ttcgatcaag tcagattcaa ttgtaccttt atctgttttg attttcattg
     1981 cagcttccat gtctgctggt ttgttttcag caacaagttc tgcaatttct tttactaaat
     2041 cttggaacat ttcgttttta gaaacaaagt ctgtttctga gtttacttca acgattgctg
     2101 cagtgttacc tttcacagca actgaagcca atccttcagc tgcaatgcgg tcatttttct
     2161 tcgcagcctt tgccattcct ttttcacgta aaagatcaac tgctttttcc atgtcgcctt
     2221 ctacttcaac taatgctttt ttcgcatcca tcattcctac gccagtcatt tcgcgtagtt
     2281 cttttaccat tttagctgta acgtctgcca tgtttaactc ctcctgtgtg tttttctttt
     2341 taaaaaaagc tgcttcaaag gagagtgagg cttgtcgccc agcctttgaa acagctccat
     2401 cattcaaaat tattctgctg aagaattgtc gccttcaaca acatctacga tttcttcgat
     2461 tgatgttgct gtgttttctt ctgtgaaatc ttcttcaact acttgatctt caccttggtt
     2521 tccttcgatg aaagcatcag ccatttttga agtaatcaat ttaacggcac gaattgcatc
     2581 gtcgtttgaa gggattacta cgtcgatttc atctggatcg cagtttgtat caaccatagc
     2641 tacgattggg atatttaatt tgtgtgcttc ttgaacagca atacgttctt tacgtgggtc
     2701 aacgatgtac attacatctg ggattcttgg catatcagcg ataccgccta agaatttttc
     2761 aagacgttca cgttctttgt tcaaaccagc aacttctttt ttaggtagaa cttcaaaagt
     2821 tccatcttct tccattgcat tgatttgttt caaacggctg atacgttttt ggatagtatc
     2881 ccagtttgtc aatgttccgc ctaaccaacg atggttaacg aagtattgtc ctgcacgagt
     2941 tgcttcttct ttgattgctt cttgtgcttg tttttttgta cctacgaata atgctacgcc
     3001 gccttcttca gcaacgctct tcatgtaatc ataagctgca tctactaatt taactgtttt
     3061 ttgcaagtca atgatgtaga ttccgtttct ttctgtgaag atatatttct tcatttttgg
     3121 attccagcga cgagtttggt gaccaaagtg tacgccggct tctagtaatt gtttcataga
     3181 aattactgcc atgtttgttt cctccgattt ggttttattt ttcctcttct tatcttctac
     3241 cttgccggca aactttttca agcacctacc gacaatccaa taagaatgtg gatttgctga
     3301 tgcataaagc accctcagct attatacaga caaaagatcc gtatcgcaag agcaaatata
     3361 cttttctgct caacattcaa tagtttacct gaaagataaa aagaattcaa gaaaaacagt
     3421 agatttctct ttgtttcttt attatccttt ttccataaaa taattttagg tataaaagac
     3481 gaatattatt gggcaatcct tctttttttt gttatgatac atgtgaaatc tttcgtaaag
     3541 gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt aaacaagtca ttgaagaatt
     3601 cccgatccgt acgcaagaag aactattaaa caaacttggg cattatggcg ttaccgccac
     3661 gcaggcaacg atttctcgag atatccgtga tctaaaaatc gtgaaggcac ctgatgaaaa
     3721 tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact cctgagacta ggactgagga
     3781 agaggatcgg ctggttcaaa tgattgaaga cattgtattg aaagtcgaac gagtccactt
     3841 tttgacgatc gtcaatacct tgccagacaa tgctcattta tttgcatctg ttttagatga
     3901 gatcaaacct ccctctattg tcagtacaat tgctggattc gatacgatca tggtcatctc
     3961 ggaaaatgag gaggacgcaa aacgagtaga agaatttctt caccatccaa ctgaagtatc
     4021 acttttttaa tcagagcacg tattcctaat atggaatgcg tgttttttta ttttcaatat
     4081 tttccttatt aaataaacct ttacactctt tacaaaaata catcattttt tattcaattt
     4141 tttcaacacc ttgattgaat cacaataatc atcatttata ttggatgtta acaagagcag
     4201 cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga aatcatatgt ataaattatt
     4261 aactcctgga ccattgacga cttctgaaac agtgaaaaat gctatgctga tcgatcattg
     4321 tacttgggat caagaataca aagaaattac acaatggata agaaaaaaat tattggatct
     4381 tgctcaagta tcggaaggaa tatacacaac ggtcttaatg caaggtagcg gctcctttgg
     4441 ggtagaatcg gtattaagtt ctgtgatcag gccagaagat actgtactga tttgttcaaa
     4501 cggcgcatat ggacaacgaa tgatcaacat gtgcgaatgt ctgaaactga actataccgt
     4561 ttatgaggta tcggaacatc aaatcctcca agctcatgtg attacacagc tgattgaaga
     4621 gaatccctct atctccgcac tagcaatggt ccatagcgag actacttccg gtattttgaa
     4681 taatttggaa gagattgctg ctgtcacaca agaaaaagat cttttgttta tagtggacgc
     4741 gatgtcaagc tttggtggga taccgattcg tgtagccgaa ctagggattg attttttgat
     4801 ttccagctca aacaaatgta ttcaaggtgt tccaggcttt tcttttgtac ttgctaataa
     4861 agcggaacta gaaaagcgaa aaaatgtgag tcgaagtttg tcacttgatc tatatgacca
     4921 gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc acttccccta cccatacggt
     4981 tttggctttt caacaagcgc tgaaggaact agaggcagaa ggaggaatcg aaactcgata
     5041 ttcacgttat ctcgccaata atcatgtact acgacaaaaa atgacagaga tggggttcca
     5101 gcctttcatt caagaatatc aggggccatt catcacctct tttctttacc ctaaggactg
     5161 gcgatttgat tttaatcatt tttatcagta tttaaagcaa aacggatttg tgatctatcc
     5221 cgggaaaatc agcgatcatg aatgttttcg tatcggaaat attggcgaga tctatccgga
     5281 agatattttg gaactgacag caattatcaa agactataag gagaacgtta atgattaaaa
     5341 ctgtaatttt tgattgggca ggaacaactg tggactttgg ctgtatggct cctgtccacg
     5401 cttttagaaa tgcttttcta gaaaaaggaa tccaactgac tgacaaagaa atccgcgaac
     5461 cgatgggaaa attaaaatgg gatcatatcc aacagcttct ttccctccct tctgtcaaaa
     5521 aacaatggat agctatttat ggacaactac ctcaggaaaa agaggtggag gaattgtatc
     5581 aacaatttga acattatctg tttaaagatt tggtcaagca ttctaccttg aaacccgaca
     5641 cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa tattggttcc acgactggat
     5701 ttactgctcc tatgatgaca atcgtagcag aaaccgcaaa aaaagaaggg tattcaccag
     5761 acttcatggc aacttctgaa gacgtcgaag ggtatggtcg accgtttcct tatatgatcc
     5821 ataaaaacat gcagtacttc aaaaacaagt cggttgatga agtaatcaaa gtcggagata
     5881 cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt gacagtaggt gtaattgaag
     5941 gtagttcgct aatggggatt tcttatgatg agtaccaaaa attagagatg gggaaaaaag
     6001 atcgtttaaa acagtctgtt gctcttagat tccaacaagc tggcgctgat atttgtgtaa
     6061 acaatttaaa agagttaaca gaatacattt tgtacgctga caagaatcca gaatgacttt
     6121 tatacaaaat cagaggtcgt gaaaaagctg tactgcatca agtaataaga acagccaaac
     6181 aaaaatagct cctcatattt ttcgtttggt taggcttatt accgcagatg caagcttttg
     6241 aacaccgttt attcgaaaaa aaaagggagc tgtgacacga cttttgtcac agtcccctta
     6301 tttacaaaca aacagtgttt aagagacaac ctctccacta aacggtgttc attcagctga
     6361 ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc tattttctct tttgtttctc
     6421 taatactcgg atcagaacgc tgatttcaca ttctcttttt cttacatctt atctggtgca
     6481 tgaagtccta gcaaacgaag gtcttctttc aataatacag tcactgcata aactagagct
     6541 aagcgtgctt ctttttgttc atcatcagct aaaatcttcg tatgagcata gtatttgttg
     6601 aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg acggttcgta tttttcccca
     6661 gcagacaaga ccgtttcagg gtatttttga actaatttaa ccacttccca gctggtatcg
     6721 tcattcaatg catagtctgc tgtttctgaa gggacaaatc ctgctttttc caacaggctg
     6781 actgcacgag catgtgtata ttgcacataa ggtccagttt ctccttcaaa tcgaacaact
     6841 tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt tcaaatcgtg gaaaatgact
     6901 gccccaaccc caacttgttt agctacagca tctttgtttt ccaaatcagg gtttttctcg
     6961 ctgatttgtt ctttagcaga ttggattgct tcatttaata cttcttctaa taagacgatc
     7021 tttcctttac gagttgataa ttttttacca ccttgcgtga tcaaaccaaa tggaatatgg
     7081 cgcatatcgt cagaccagtc aaagcccatt tccttcaaga cagctttcaa ctgtttaaaa
     7141 tgatagcttt gttcattccc tactacatat agagattgtt tgaagtcgta agtacgttta
     7201 cggtacaatg cagcagctaa gtcacgtgta atgtatagtg tcgcaccgtc tgacttctta
     7261 atcaacgctg gattcaggtc ataagcagat aggtcaacga tctctgcacc tttatcctcg
     7321 ttcagcaaat gtttttcttc taaaagtttg acgatttcat ccatcttgtc attgtagaaa
     7381 gcttcaccgt tcaacgaatc gaaacgtact tccagcagat catagatttt attgaattct
     7441 ttcatcgatt catcacggaa ccattgccat aattgaatag cctcttcatc gccttcttcc
     7501 agacgtttga accaagcacg agcttcttca tttaattctg gttctgtttc agctacttca
     7561 tggaattgga catataaacg aagcaattca ttgattggtt ctgctttaac agcttcttct
     7621 gtaccccatt ttttgtaagc gacgatcagt tttccaaact gtgttcccca gtcacctaaa
     7681 tggttgatgc ggattggctg ataaccgatt ttttccataa tgaaaccgat cgaattaccg
     7741 ataactgtcg aacgcaaatg tcccattgag attggtttgg caatattagg agaagacatg
     7801 tcgattggta ctgttccttg attcccgata ttgctatccc cataatgttc tttttctttc
     7861 actactgttt gcaaaacttt tttgctgata agttctttat tcatgaaaaa gttcaagtaa
     7921 ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga ttttttctgc taaatctgca
     7981 gcgatttgct gtggcgcttt tcggtaaact tttgctaatg aaaaggctgg aaatgcaaca
     8041 tctccgtgtt cagctgattt tggattttct agtaattgtt cgacttgttc cagtgttaaa
     8101 tcatctttga cagcgtcata gacagctttt gcgacaagat ctttattatt catctttatt
     8161 cctcctaaag tgctcccttg attgttaaac aaaaaaaatc tctagcgacg gcatacttag
     8221 tatgccatca ctagagacga gatttcccgc ggtaccactc caattgtcct aaaaaaggac
     8281 cagctccatc tgatatcggt agacgaccgt ctgtttaaaa cagaattctc caagtgcgct
     8341 tcgttgtatc ttcttcattc ggcttccacc acttccgaat tcgctagcca agaatgatac
     8401 aagtactctc ttgttcaaca aatcttagtc ttattaaact ttcttgaatt atatcagaaa
     8461 aaaaaacgaa aggctagtcg tttctgataa aaaaacaatc cagatggcct aaaggagaag
     8521 aaataccacc tggatagcta taaatcattt cgtaaatacg aaagacttaa caaattaatc
     8581 agctacgata atcgttcctt cttcagaagc taaaagattc tcgatatttt ctaaagaagt
     8641 gatgattgcc ttagcgtttg gttttgcttc aacaaatgca atagctgcct ctactttcgg
     8701 aagcatgctt cctggagcaa attgcttttc atcaatgtat tgtttcattt ctgaaacagt
     8761 aacagtttct aatttctttt gatctggttt ttggtaattg acatacacat tgtctactcc
     8821 tgtcaatacg atcagcaaat ccgcatcgat gatttcggct aatttttctg aagcaaaatc
     8881 tttatcgatt acggcttctc gtccttctaa tccagtagct gtttctacaa caggaatccc
     8941 accgccacca acagagactg tgataactcc ttgatcgacc agcgtttcga tgacacgtgc
     9001 ttctttaatc gagatgggtt taggggaagc gactactttt ctccatccac gtccagcatc
     9061 ttctttgaat gtcacagtgg agtcagcatt catttgttct ttggcttctt cttccgtgta
     9121 gaatggaccg actggtttac ttggattttt gaatgaagga tcattttcat ccacaataac
     9181 ttgagtgact aaagaaacca catctttatc gatccctttt tccttcagta cttcgcccat
     9241 ggcattttgt aaccagtaac cgatagatcc ttctgtcatt gcaacacatg tatctaaagg
     9301 cattgccggt gttttctcag aatcagcagc ttgctgctga atcaataggt taccgacttg
     9361 cggaccattt ccgtgggaga tgatcaattc atctccttgt tcgataaatt tcactaaata
     9421 ctttgctgtt tccattaacg cttcttgttg ggcttttgca cttgcatccg tagacaagat
     9481 tgcattaccc cctaaagcga caactacttt tcgtttggcc atttcattcg tcctttcttc
     9541 agttctaccg aattttctag ataacaaaac gagccagcgt tttgtgattt tccctcaatg
     9601 ctgactcgtt ttttcgtgag atgaatgtgg ttatgcttaa acgcgaggga taaacaagtt
     9661 acctaatgtt gctgccatga tcgctttgat tgagtgcata cgattttctg cttggtcaaa
     9721 ttgacgagca tatttgctgc ggaagacttc atctgttact tccatttcag tgatgccaaa
     9781 gcgttctttc atttgttcac cgtaaacagt gtttgtatca tggaaagctg gcaagcagtg
     9841 caagaagatc aaacgatctg tgttgtgtgt tttttctacc atttccatat tgatttggta
     9901 aggtttcaac aatttgatac gttcttcgaa tttatcttct tcacccattg atacccatac
     9961 atcagaataa aggacgtctg ctccgtctac acctttatca acatcgtcgg taatcatcaa
    10021 ttgtgcaccg gatttttcag caaagccttc tgccattttg acgatttctt cttcaggttg
    10081 taattctttt ggtgcaacga tacgcatatt cgctcctaaa attgcacctg tgactaataa
    10141 agagttagcc atattgtttc gaccatctcc gcagtaagca acagtgatac cttctactgt
    10201 tccaaagttt tcttggatag ttaaaaagtc agcgatcatt tgtgttggat gccattcatc
    10261 agtcaagcca ttccaaactg gaacaccaga gaattctgct agttcctcta ccattttttg
    10321 actgaatcca cggaattcga ttccatcaaa catacgacct agtactttag ctgtatcttc
    10381 tgtggattct tttttaccta attggatatc atttgcacct agatattcag gatgtgcacc
    10441 tagatcgatc gctgcagtag taaatgcaga acgagtacgt gtagatgttt tttcaaataa
    10501 aagcgcaata ttttttcctt ctagatagtg atgtgggata ccacgttttt tcaagtcttt
    10561 caagtgttct gaaaaatcga tcaaatattg taattcttct tttgtaaagt ctttttctgc
    10621 taataagctt cttccttgga atactgattc tttcatatta aacattctcc tttagattgt
    10681 ttttatttaa gattaaagag gaaatcccga cggcagctga cagtaagacc gccgtcagga
    10741 aacaaattac ttcaagtctt cacggacaag tggttggctc atgcagcgtg gaccaccacg
    10801 gcctcgtgac aattcacttg aattgatttc taacactttg atgccatagc tacgaagcaa
    10861 ttcgttcgat acataatttc ggttataagt gactacaaca cctggagcga ttgccaatgt
    10921 gtttgatcca tcattccatt gttctcgagg tgcaacgatc tcatctccat taccagttgg
    10981 gatcagtaca agatcatcta atccaagagc agctttcatc gtttcgtgta agttatctga
    11041 atgagtaatc ttgatatcat caccatctgg ttcgattgta aagacatcca tcttaccgtc
    11101 tttactttga attgctggat ggatagtgaa tttatcgtga tctaccattg taaatacggt
    11161 atccaaatgc atcattgcac ggttgtttgg aattttgatt gctaacactt tttcaaatcc
    11221 tgaatttttt gcgaataggt ggcgtgctaa tttttctaaa gctttcgcat tagttcgttg
    11281 agaaatccca acagctacta ctttatcgct taagatcaat tcgtctccgc cttcgatatg
    11341 atctggattt tcacgatcca accatacttc gattccttta tttgcaaatc ttggatgatg
    11401 tttcaaaata tattctgtaa acatcgattc acgacgacgt gcttcaaaag tcatcttatt
    11461 gactgtcatc ccatttccca ttgatgcgga agggtcgcgt gtaaagtaca agtttggcat
    11521 tgggtccatg taaaatggat agtcatcgtc tgctgacaga tcgtatagac tgctagaacg
    11581 tacttcaata tcttttgtac gaaccccagc catgatttta tctaccattt cttgcgtttc
    11641 cattgataga aggaactcat gaagaccctc tcttacagca ttcgatgcaa tatgagattc
    11701 atccaacatt ttattcaaga attgttcttt gacattgcct gcgtcaatcg cttctgccgc
    11761 taatttttcc aagtaaagag tctcgacgcc ttcattttgc aatgtctttg caaagttgtc
    11821 atgttcttct tgagcaatcg gaagatatgg aatatcatcg aacaacagac gatccataat
    11881 atctggtgtc aaattttcca cttcttgtcc aggccttttc agtaaaactg tctttaattt
    11941 ccctatttca gagaaaacgt gaataggttt atccatgttg tgttgcctcc ttctttttga
    12001 ttacatgtat aggatatcct ccttttgtaa tcactttcat aattttgtct cattcaaaat
    12061 gagacattcc tcacattaat attcgataat taaataatta aacgtttttc tatttttaag
    12121 cgtaatttat tcatttttta ttctgtatac actttcaaaa aaatatttat tcattgaaaa
    12181 aaattttttg aataaattaa aaaaataaat tatattagcg ttgttagcgt ttactatatg
    12241 tattattgtt gttttttata cacaaatcat tcgcttcaaa acaaattatt caataccatt
    12301 gtatattttt tacataataa caaattatat ttgtaaagta ttaaaacttg tttctaatgt
    12361 tggtatgtta aaactttaca aattctttaa aaaaagtgac aataaatata gtgaacaata
    12421 aggagaggac tttttaatgc gtgttaataa aatcgatttt cacttttcaa aactcagata
    12481 tcaacctgat tttgaacatt tctcggatga agagttccaa cagatcaagg aacatactgt
    12541 tcttcgttct tataaaaaag gacagatcct tttcgacgat ggtgatccca gacaacggtt
    12601 gtatatcttg accaaaggac tcgttcgttt agaacgttat gatgaaagtg caacttatta
    12661 ctactatgac tatgtgaata caaataacct ttttcctatg tcaggaatgt ttggagaaga
    12721 gacctacact tatacagctc aagcgatgac tgatattgaa acattctacc ttcctgtttc
    12781 ctactatgaa aaattagcac gaaaaaatcc cgaacagttg atctatatga tacaaaaaat
    12841 ttctaaagta gttgaaatac atgaattgcg tatccaaatc ggattgacct ctagcgcctt
    12901 tgaccgggta aaacaaaatt tattcatttt gaaagaagaa ttgggcgtac caacaaaaga
    12961 tggtcatatc accattcctt atccgatcac cttaaaagaa ttagcagtga atagcggcac
    13021 gacacgtgaa actgctggac aagtaatcaa acaactacgt gattccggac tgttagatta
    13081 tgacaaaaag cgttttactt tctgtaagac agctactact ccagctatga gagaataacg
    13141 cgaacaaaaa taagcagtga ccctgatatt tatcagggtc actgcttatt ttcctcccta
    13201 aaacttctga aagatccggc agttttagat cactgggcaa gactcaaaga ttttctatgt
    13261 gtgtttcagt tatgtaatat tcactgtttg caaaaaatac tgatagatta actagaggaa
    13321 gcgtcttgtc ctcatccgac ctagattatt cgatattcct cgacaataaa cgattaatca
    13381 gtgagggcaa tcagaggaac ggataatgat gaataagctg taaaaaatag ggctttgaca
    13441 aaagcgtgtc acagccctta tttcgattaa acggtgtgta cctgtcaact cttcggtcac
    13501 aagtcataat gttcataaac atgaggagct gttttttgaa attgttcctt gtgaatcaga
    13561 gttaaacgac aggcttacga actcttgatt aaacggcgtt caaaagcttg catacgcggt
    13621 aataagaaca gccaggtcaa aaacatgaga agccgttttt cctgggctgt tcttattact
    13681 tgatgcaggg cagatttttc acaacctctt tattcagata aaaccaagct tggatcagca
    13741 ttcaactgat agctagcaaa atttcttttt tgcatttcca catgtgctgc tgctccaatc
    13801 attgcagcat tatctccaca aagtcgaaga gggggaatca ctagttctac ctcagggagt
    13861 ttagttgcca aagcatcctg caatccttca cgcaaccctt gattggccgc tacacctcca
    13921 gctacaatca gttgcttgac tggataattt tgacaagctc gaagtgtttt agtaatcaat
    13981 acatcgatca cacttgcttg aaagctagct gctaaatcat ttttgtctag atcttctcca
    14041 cgctgttgtg cattatgcac caaattgata aatgcacttt ttaatccgct aaaactgaaa
    14101 tcataattat cttcatggat catcgctcgt ggaaaatggt aattatcttt tccctgatga
    14161 gctagctgat cgatttcttt tccgcttggg taagaaagac ctaatactct tccaactttg
    14221 tcataagctt ctcctgctgc gtcatctctt gtctccccaa tgatttcata tgatccatct
    14281 tcttgcatat agactaattc tgtatgtcct ccgctaacaa gaagtgccat caatggaaac
    14341 tggaatggtt taaccagacg ggctgcatag atatgccctg ccatatggtt tacaggaata
    14401 agcggcaatt ggtgtgccca agcaaaagcc ttagctgcag aaataccaat cagcaacgag
    14461 ccgacaagtc ctggtccata agtgacagcg actgccgata aatcttctgc cgacacacca
    14521 gcttctacca gtgcatcttc caagcataag gtgatttgtt ctacgtgatg gcggctagct
    14581 acttcaggaa ctacgccacc aaaacgttta tgactgttta tttgagaagc aacgatgtta
    14641 gataagatct ctgttccatt tctaacgacc gctacactcg tttcatcaca acttgattca
    14701 atcgccagaa tcaattcttc attcatttca tcgtctccgt ttttaacttt ctgctcataa
    14761 tgatcgcatc ttccgtcgga ttgcgataat atgatttccg tttacctaat atacgaaact
    14821 tttctgattc ataaagtttt cttgctgctt cattagatag acgtacttct aaaaaaacat
    14881 gcagcgaatc ttttatttgt tccgtaatca gtaattgtaa caaatgccgt gcatgccctt
    14941 ttccttgttc tgaaactgca acagcaatat ttgtgatctc gacttcatct attactttcg
    15001 agtatccaat aaagccacag atctggttat cttcaactaa aagcagataa tcagagtgag
    15061 gctgttggat gtctgccaaa aactgcattt tcgtccaagg tgaaccatgt ggataagcat
    15121 tttcgctaat catccatacg cattctgcca agtacgcttc atcaaaatct ttttttctgc
    15181 aaatcaacta atctcactca ttttctaaaa gttttatcat atccaacgca tcttcttttg
    15241 tttcagtgta atatcttttt ttgatcgttg ttgcttgaaa acctaacttc cgataaagtc
    15301 tttgtgcatc ttgattactc atacgcactt ctaatgacat cgtctcacag cgattcatta
    15361 tagcaaagtt ctcgatttcg tcaataagca gcgaaccgat tcgtttacgc tgatatgccg
    15421 gtcgaacggc aatattcgtg atatggcaat ctaaaccgaa cgctcttgaa ccgataaaac
    15481 caatcattct tccattatca aagataccta gatatagatg aggaagcggt gacttcaatt
    15541 ctgatataaa ggcacttttt gtccatggca attcgccatt atagacttct cgttcgacat
    15601 ccagcagctc tttgatatct tctgtagtca attctcttag tacgtaacga cgatcgattt
    15661 gttcgattgt tttttctgta taaatcattt tatcttgaaa aaagcttctg gcatacgctc
    15721 taaatttttt caacataact ttcgtctcca ggtgtgtgcg tctccaacca tttttcttct
    15781 gcttctaccc gttttaaata gcgaggaaga aaagcttgtt tctctgcaac tggttcagct
    15841 aaagcaccta attgagccac agttacacca cttggaatat cccatgctgg aactgtatta
    15901 atcatcgcac taggcaatat ttcctttatt tcatagaaga atttgtgaca atcactcccg
    15961 acgaaataaa tagcttcatt cagcttttgt agttgtgtga acaaatcaga agcagaaata
    16021 tgtacatctt caagtacaat ttctaatcta ttttctttcc agcggtatgc cccagcgtat
    16081 acattttttc ttcgagcatc aaacatgggc acaataattt tatccacacc gatgcaatta
    16141 gccgcgatgg ttttcagact agatactcct actagctctt tatctaatgt ataagcaatc
    16201 gtttttgctg tcgttacacc tatacgaagc cctgtataag aaccgggacc atcagaaacc
    16261 gcgattcggt caatgtcttt tggacttatt tgaagatctg caaacaattg gtcgattgca
    16321 ggcatcagtg ttacactgtg gttgcgtttg atatttgttt gtatttgtcc aagaatttgt
    16381 tcgtcttcta ccacaccgat cgccaaagtt tggtttgcag tatctattcc taatgtaatc
    16441 atatatttga tcctttctgc tatgacattc tcgccttcca ttgtagcata cttttttatt
    16501 ctgaggatga aaataccaaa ggcaaaacta gtacaatgat acatgattat ctgctggcgg
    16561 gcaaattttg ccccttcctg aaacacaaaa aagctgcggt atcacttcat ttccccacag
    16621 cttttccatc ttttatttcg ttgattctcg aataatcaac tctgtttcca caataatctt
    16681 tcttgaaacc ggtgctttgg aattacttaa ttgaagaatc gtctccactg ctagctctcc
    16741 catccattcg gtataaactt ttacagtaga aagagagggc gttacatatt tcgctacact
    16801 gacatcattg aaacccatca cagaaatatc ttctggtacc actaaacctt tttcttgaat
    16861 cgctttcagt gcgccaatcg ctaatgcatc attagctgca aaaagcgcat caggaacatg
    16921 cggattcgtt tttaaatact gcttcactgc ttgatatcct gactcgacag aaaaatctgc
    16981 ttcgataatt ggaccttcgt cgtagccttt ttctgccaaa acttgttgaa atgccataat
    17041 tctgggatct tttagccgct gatggttttt cttcgtatat tctgttccag caagcatagc
    17101 gatatgttgc gctttatggg agacaaaatg ttcaatgact ttttgaacac ttgacttaaa
    17161 gtcgacaact agagagtcga ttccttgaga agtgccgtca gaatctacca aaagcaaggc
    17221 attttgggat cctttcattt ctaataactg agcttcgtcg aattttccta gtgcgatcgt
    17281 tccatctgtc ggaagatcac taatggaatc ccacgtttct ttcaataaag taacgtttgc
    17341 ttcttctgcc tttttttcga ttcctagacg aatagataaa taataaagat cttcaagctc
    17401 ttccgtatcg tcgtaccact ggatcaggcg aagcgtttgc ttagtatttg ctgcattttt
    17461 acgatgtttc gtataattta agcgttctgc tgcttcaaaa attttttgct ttgtggctaa
    17521 gccaacggaa agatcaggat catagttgag aactcttgaa acagtagcag gagaaactcc
    17581 tgccaactgc gcaatgtctt taattgttgc catttgtctc tcctcctttt ttacgtttat
    17641 aaaattgcag taaatcgttc aaacgctgcc tgtccttttt catctcgctt gaatactcca
    17701 gcgtcttcca gtacacgtgc aaaaacctga cctacagatt tttgtacgac atcggtgacc
    17761 gtatctttcg tcaaatcagg atgttctttt tttaattgtt ctgcccacgg acgatgatat
    17821 tcagccatct ggttttcttc tcctaataag aaatgctcta cttctactag ttctggtttc
    17881 aaacgaggtg gtaaaacagc aagccccatt acttcgatca acccaatgtt ctcctttttg
    17941 atatgttgga catctttatg tggatgaaaa atcccatcag gatgttcttc tgaaacattg
    18001 ttgtctcgaa ggacaaggtc caactcaaag taattgtccc ttcttcgtgc aattggcgta
    18061 atcgtatggt gtggtgtacc atctgctgaa aaagctctga tagatactgt ttcatccgaa
    18121 tatgctttcc acttttccaa tactgagtta gctgcttcaa tcaacaattt tttctctgtt
    18181 gattgcaaac ggatcacaga catcggccat ttcacgactc ctgcgatgac ttgcggataa
    18241 tctgctagtt tgataagttt ttctatcggt gcttttgcca taggaaattc atgacgtcct
    18301 gcctgataat gatcatgaga aagaatcgat cctccaacaa tcggcaagtc agcattcgaa
    18361 ccaacaaagt aatgaggcaa gacttcagta atccgaagca gtctagcaaa tgtttccttt
    18421 gtgattttca tcgggcggtg ttgttctgat aaaatgatcg aatgttcatt gtaataagca
    18481 taaggagaat attggaatcc ccagctttca ccgtctagat tcattcgaat aatccggtga
    18541 ttggtgcggg ctggatagtt caagcgacct ctatatcctt cgttttccat gcaaagcatg
    18601 caggtaggat agtctacttt taccgcctcg cgttctgctg cgatttgttt tggatctttt
    18661 tctggcttag ataaattgat tgtgatttcc agttcaccat actccgactc tgccggaaat
    18721 ttgatgtttt ttgcgatggc tcttgttttg atgtagtcat ttttcttact taaattaaag
    18781 aaatactcag ttgcttcctc tgggcttttc gaatagtgct gtgcaaagaa tgcattgacg
    18841 actgaaggtg gcggtgtgag aaaatccatt aattgcgctt caaaaatctc ttgttctgct
    18901 ggcaattctg aaagaacacc attttctctt gccttagcga caagctgatc caataagtct
    18961 acagattctt ccttaactgg tcttacttca atgctttcta gagcatcttc tcctaccatc
    19021 cctaaaagac gattctgtaa atataagcgg tccatttcca tccatccgcc agcttcgatc
    19081 gcaagtgtgg caaaatcaca aatcgtttga ctgattgaca tatctcattc ccctattctt
    19141 cataaccatt tggatggctg acatgccaat cccatgctgt tttgataatt gctttgatat
    19201 ctgtgtattc aggtttccaa cccagaacct ctctcgcttt ttcacttgaa gcgactaatc
    19261 ggctaggatc tccagctctt cttggtgcta tttctgctgg aatttctttt cctgtcactt
    19321 ctctagctgc ttctagcatt tccttgactg agtatccttt gctgctgcct aaattgaaga
    19381 agttgctttc atttccttct ttcaagtatt ccaaagctaa aatatgtgca gcaatcaaat
    19441 cttctacttg aacataatct ctgatacatg ttccatctgg tgtatcataa tcgtctccat
    19501 aaacagccaa tgcttttctt tgaccaagag ctacttgtaa aatgattggg acaagatgcg
    19561 tttctggtgt atgatcttct ccgatagatg catctgcttt tgctccggct acattgaagt
    19621 agcgcaaagc gacatagcgc atgccatatg cttggtcaca ccacttcatc attttttcca
    19681 tcatcaattt actttcacca tacggatttt taggattagc tggtgtatct tctgtgattg
    19741 gagactcttt tggttctccg taagttgctg cagtagaaga gaacacgatt ttattcacat
    19801 tgaactcgtg catgacttca agtaagattt gcataccgta tacgttgttg ttgaaataca
    19861 tcaatggttt ttcgacagat tctcctacta aagagcttgc agcaaaatgg attactcctt
    19921 caatcggttc cttttcaaat acggaacgta gaaattcttt atcccggatg tctccttcat
    19981 aaaaatgagc atctggatgt actgcttgtt tgtgacctgt gagcaaatta tctaccacag
    20041 ctacttgata ccccttttga actagctggt ctacagcgtg cgaaccgata taaccggctc
    20101 cgcctagtac taaaattgtc ataaaaaaca ctcctttgat tagtaaaatt gtgtttgttg
    20161 attatatata atttaaaaaa acgaagttta cttttatggt gaaaacaaac aaagtaaaat
    20221 ttcgtaaatt catatggtaa atttataata aaacatttag taaacatttt caaggatttt
    20281 ttgcaaaggt ttacaaacaa acgtaaaact agcctttttt tctcttttgt tccataatag
    20341 atataacaaa taataaatga ggtggattca atgaaaaaat ttgtatctgg aatattagta
    20401 ggaagtctgg caacagccgc tgccgttgct ggtttggtcg cttctgtgaa aaaaacagtg
    20461 atcgatccaa ttgacgaaaa agaagcaatg atcgaagaaa accgtaaaaa agcaatgcgc
    20521 aaacgcgttt ctcgttaata attatttaaa aacacgcatg aagacttgtg aaagaacaga
    20581 taagtcatca tgcgtatttt tatttgtcag tcaatataag ttgtgctatt ttatctgcct
    20641 gtctcagatt ccatggatct gtataaggtc tagctactgc tgcagttagc caatccacac
    20701 cttcaacagg tattccatag caataaaagt cagactggat ttcccctgaa gcatcaatca
    20761 cgcgattcgt acctcgctca acatctatag cttctgaatg ataacttcct gtattatctg
    20821 aaaatgaaaa agggcgaaca agtttgtttt ccttcaaatt ttttatgaca ggattacttg
    20881 aacgagaaaa gcttgtttgt ggcaatcgag cttcaatcaa atagcgactg ctgatttctt
    20941 tatcttgtag agaaataaca tagcggttgt gcttttgctg aatatcaaat ggcggttcga
    21001 acaaatgaaa aatacccgca tctatcagtg ctgctaattc tttcgtccga atgactggcg
    21061 gaccgatcgt taaaaagcta tttagatgag taaaccatcc ccatagtatt tctttgtatt
    21121 cctttgcaga aaaaacgtgc cattcaatag cctgccggat tggatcacgc cagtctttta
    21181 atgcatcaaa tgttgaagca aatgctcctg tgcaattgcc tttttccgct tcttttatct
    21241 gcccttcaat aaatgctcgg ctagcttctg caaaggactc atcctgatag gtaagaggaa
    21301 tttctaaata cgaccaatcc catctataag ggattagtgc tggatgcttt ccgagacact
    21361 cctctgaaga gagggagaga aaatcatgct caaaattttt ctttggaatc ggcaaatgat
    21421 attcttcgat gattttcttg taataaaaca actcaacgtc ttttttcaga tagtcaaaaa
    21481 atgtttcgcc agtcaaaaat cccgactgat gccattgctc caggttttct tttgttagta
    21541 accgtggcca tgccattgat ccgccttgct tttggtttct tcctcgaggg taataaggga
    21601 gcccccttct tgatccactg tatacaatcg gttccttgcc tgatggatga tagacaagtt
    21661 ttcctttttc gttctcgaag cgcccttctc ttgatactgt aaacaatccg acataatcaa
    21721 aaaaacttaa gcctagacct cttaaaaaga cattttcttt aggcggtatc ttcgaaagat
    21781 cgacatctgc aggattagaa ggaagctgat acagtaaatc atttttcttt gcgtagtcta
    21841 cccacactct ttcttcttct gtctcatcat tttcccagtg accgcttgct aaaacaagct
    21901 gatcgataga atagctttgc tgatctgtct gtaaaagata accatctttt cttttgctga
    21961 ctttttttac taaagaataa acaaactgaa tagaagctgg aaattcttct tgcaactttg
    22021 agaaaaacca gcgttgatag agtccgtata aacatcgagt agattgctga tctttttcca
    22081 gacgattgat ttcttctata attagttgct tatttttggt taccgtctgt tcgatataat
    22141 ctgtagcagg tcctttactc cattgaaaca agttgggtcc ctgtgttacc aatcctccac
    22201 tggacaatgt ttcatctgta aaaagcgtca cttgttgaga aacagaattc attaataact
    22261 ccggtggctg attcaaccgc caaactcgac ctccaggacc attgggatcg aaaagaaaaa
    22321 gttcaagaga atgtctgtcc tgattattat ggagaatcct ttctgctata cttaatcctc
    22381 taggtccggc accaatgatc cctattttca tttgcatgcc tccatttcga taaatttttt
    22441 caataaaaca gcatggttct gatgttcatt ttttgcacca taaacaaaag taatgactgg
    22501 atgttctttc ataagttgac tgagtttaaa aatagcttgc ttcttttctt cttcgaaaat
    22561 caattcttgc tggtatttct ttgcaaatga agcaaatcgt tctggtacat ggtcaaaagt
    22621 tttgcgtgct tcattactag gtgcaatttc tttcaaccac agatctaact gcgctttttc
    22681 ttttgaaatt cctcgtggcc agaacctatc cacgagtatt ctatagccat cctctttttc
    22741 agcatcaagg taaacacgtt ttaatttgat catagttgtt tccttctttc tgccttttcg
    22801 cttagcttat atatgtagat aatagcacaa aataaaaaaa atgaacggca caactatcca
    22861 ttgcgccgtt catgtgatct tggtcaaaat ctaatgattg attcggtttt taaagctgtc
    22921 atacaatcat ctgttttata cttttttact tttgattttt ccagaccatc cgtcatatcc
    22981 acccttaagt aaataaatct cttgataacc tgctttacgt aatttatttg ctgtacggat
    23041 gctcaagctt ttcttttgat cgtaaatata gacaggctgg tcttttcgaa tagatcctaa
    23101 tgaatctttc aaaacagtgt aagggatgtt tcttgcacct aaaatatgcc cagcatcaaa
    23161 cacatctttt tcgcgaacat cgattacttg tgcttttctc attccttctc gaaattcttc
    23221 ttctgtcaaa gtcgttgctg aacgcttggc cattactcgt agatagactt cccaaaagat
    23281 gattgcaagc acaatcaata gcaaaatgat attaattacc cataaaacca tgtaatgcct
    23341 ccgttttttt aagcaaattt caaggctaat gaggcagcac caatgacacc agcttcatta
    23401 ccaagctctg ctaatttgat ttttgttgat tctcttactt gcgggaaagt aaattcttta
    23461 aaatatttct ctacacggct acgtaaaaat tctcctgctg ccgatactcc tccacctaac
    23521 acaatacttg acggattcaa tgtattcccc aagttggcgc aagctaaacc taagtagaag
    23581 catacgcgat caacaaccat caatgcaaac ggatcgtctt cttgagccat ttcaaagata
    23641 tctttacttg ttacttcttc accgttgtct aacattgctt tcagttttga atctcctgcg
    23701 tattcttctg ccaagtaacg acctagacgt accacaccag tagcgctaga cacagtttct
    23761 aaacatccgc gtttaccgca agtacattcg aacccttcag gatccacagt gatatggccg
    23821 atctctccgc ctgctccgct cacgccgtga atcaaatttc cgccagcaac gataccaccg
    23881 ccaacacctg ttcctagagt aacgaataca acgtctggat cgttttctcc agcacctttc
    23941 catcgttcac ctaaagcagc aacattcgca tcattatcga tagcgaattt gattcctgtt
    24001 cctgcctcga tcagtttttt cacaaattga acttctgtcc agtttaagtt atacgcacca
    24061 ataaccgttc cagcttcgct gtccacgctt cctggggtac ccatgccgat tccgataaag
    24121 tcttcggctt tcatattata taaattcaaa cgatggttga ttgattcaat gatttcagga
    24181 acgatatgct taccatcatc caaaatattg gtatcgatgc tccatttttg ttgaatctct
    24241 ccttctggtg ttaaaattgc aaattttgct gttgttccac ctaaatcaat cccaataatc
    24301 ttcttgtcca ttacaaattt cctttctttc ggctttcttc aattcggtgc tctcttttta
    24361 atatgcgcca cgctgtcatg tatgtatcat gatcaatcaa acggctatca tacagattac
    24421 gcaattcgat agtcattaat tcaatgtcat agatgcgtgc acccacatag ataaagatgc
    24481 caaatttttt gaacagctgc tgtacatcat ataagctctc catctttaag cacttccttc
    24541 atattataca agtaaaccat atttttttaa acccagtaaa atacatatta caaaaagaaa
    24601 tacaaaaact agtgtagaaa gaatcctttg gtgtatcgaa tagcttgtct gttgtttcgg
    24661 tagacccaaa acattcccta acactaatcc gccaattagc ccaccgacat gtccccaaac
    24721 atctacagag gtatccagaa gaccaaaaac aaatgtcaac acaataaata aaagaaaatg
    24781 tctgactagc tgctggatcg ctgggttatg tttaaaatga aaccctaaga taaacaacgc
    24841 accgaacaaa ccgaatatcg aagtactcgc accaccagac agaacattcg cttcgttaaa
    24901 agcaaagctt gctgtatttc ccattatccc gctgaacata taaatcaaga aaaaacgcca
    24961 atggccatag attgcctcga tttgctgccc cataaaatac agtatcagtg agttcattac
    25021 gaaatgcatc agtccaaaat ggatgaagat tggtgtcact aaacgccacc attcattgaa
    25081 gtgaacaaga taaggaccat acattccggc ataataaggg atctcaagtc gcggaaataa
    25141 ttccatcatg aggaagaccg ctgtctgtat ccctaaaaaa gcataggtcc ataacggtcg
    25201 tcttaaccat gcacgcaatt ttatttgctg ttgataattc atgaaaacct cccatttcat
    25261 tttagtcaat aaacaattgt tggatcttct gatcaaagct ctccggttgc cattgttcat
    25321 tcaattgttc acgaaaaaca aggctacatg tttttttatg aaaattattt aaataccggt
    25381 cgtaataacc accgccaaaa ccgattcgat aaccggctct attgtagata atacctggta
    25441 caatgaggag atcgatcatc tccggcaaat agagcttctc tgattgtggc tcaagaacac
    25501 caaaactcga acgtacaaga acggactgtt catccatttc ataaaacgcc atctgacgtt
    25561 tcggcaaagt tttaggtaca actattttct tttgttcttt ttctgctcgt tggataaccg
    25621 gctgtgtatc cagttcaaat tcattcgata aagtcgtccc tatcactttt gctgacttcc
    25681 agtactcgct ggcgaatagc tgtttataga ttcgctgttc tttttccttt ttcttgccac
    25741 ttttggccag atttttcagc ttttcgattc ctttccggcg taatcttact ttcaattggt
    25801 ccaaaaactc acccttcctt ctctcataaa atggtatagg aaaaaaagaa aaatagcaat
    25861 cgaactttca aataaatgca taaatagaat aaaaagaaga cctagaaatg attggccttc
    25921 cttttttaat aaatttcaac tgttcagcgc gtgtttttgt ctgataaaaa gtgcatacag
    25981 aaaatatggc gcaccaaaaa gggcaatcaa aattcctgta ggtacttccc cacttggtag
    26041 aatcactcta gcaaccatat ctgcactaag cagaaaaatg gaaccgaaca aaccgctcag
    26101 aacaaaggtc cagttatttt ctttacgtac gactagcttc gcagcatgag gagctagtag
    26161 tcctacaaaa gataaactac ctgtcaccgc tacactaccg cttgccaaag caacagctaa
    26221 caacaaaagg attttttgac ttttagccac gttaaggcct aatccttgcg cgcgttcttg
    26281 accaaatgat agcaatccaa tcctctgtgc ataaagtcct cctagcggaa ttaagataac
    26341 cgcccatgga agcaatgccc aaacataagg ccagctcgca ccccagattg atcccgctaa
    26401 ccatgcattc acaaatccat aattttcctt agataattgg atcgtgcata gcaatgttaa
    26461 tgcagaaagg ccggcattaa cggcaatacc ggacaataag attcgattca tacccaaaaa
    26521 gccatttcga tcataggccg caagatagac acagcaagca gctaaaacac cacctagaaa
    26581 ggctacaact agcaatagaa acggcagcga atagtgcgaa aagaaaccta aataaagcat
    26641 cactaaaaat cctgctccgg cattaatgcc caatatacta gaatctgcca attcatttct
    26701 tgttactcct tgcaagatat aaccagaaac gccaagacaa gctcctgcta ataaactgat
    26761 cagtaatcga ggaactcgaa attctctaac aatcagcgcc aaagaagatg attctttccc
    26821 tatgaacgtg gcaaaaagct gatccaggta aataaaaggc gtaccaatca ttaaactacc
    26881 tatagacagg atgacaagta caaaaagcaa gaaatagaac gtccaatata tttttttcac
    26941 cgttgttccc tccttacttg tatcagtaaa aacggaacgc caatcaatgc aatgactaat
    27001 ccaaaaggtg tttcaaaagg gggattgatc atcctcgctg taaaatccgc caacataacc
    27061 aatccaccgc caactaaaaa agagacaggt agaacataac gatagtccgt tccaaccatt
    27121 ccgcgaacaa ctggcggaac caacagccca ataaatgaaa caggtccgac taacgaaacg
    27181 gcaatggctg ctaacactgc aactagacag aaaacaatca accgcgttcg tttaggatga
    27241 cttccaagag ctaatgcatg tgtttcactc aaatgaatca aagatattgc ttttcctaat
    27301 gaaaaagaaa ccaaaacagc aagtatatat aaaggaaaca atactgctaa ttgtttccat
    27361 gtgatattgg cagctccgcc tacaaaccaa aaagcaagat cctgctgcag attaaagaat
    27421 tgggtaagtg cttggctgat agcagtaaaa aaagaactaa tagctacgcc agctaaaatc
    27481 agctgtaaag gattcatgcc cattttcgaa aactgcaaaa aaccaaatac gagcaaagtt
    27541 acagcaaaag aacctgccaa tgacattaga aacacagaaa aatcagaagc tttaggccaa
    27601 aaagcaaaac taagggccat tgccaacgaa gcacccgcgt tgattcctag taagcctgaa
    27661 tctgctaaag gatttcttgt cactccttgc atcaatgcac cgctcaatgc aaaacaactc
    27721 cctacaagaa atgctcccgc cacacgaggc aaacgaatat ttcgtatgat ttggtgactt
    27781 tgttttgatc tatcaaaatc aaacaacgcc tgccaaaaat ctgcttttcc cacagtcgct
    27841 gcgccatttg aaagagcaac aaaaaaaata atcaaaagaa caaaacagac caaaagcagg
    27901 agtctatttt tcttcatagt cctcataatt gatcctcctg ctcttttggc aacaaatcat
    27961 aggttagaaa aactggttta tttgttcttg gaagcgtgac aatcgttgca tcaatctcaa
    28021 atattttttt catacactct tttgtgatca cttcttcagg tgtaccttct gtgatcaact
    28081 taccgttttt cattgctaat aaataatctg aaaaacgtga agcatgatta atgtcatgaa
    28141 tcgacatcaa gatcgttgtt tgtttcattt gattgatttc agtcaataac tgtaggatct
    28201 ctaattggtg tgccggatct aaaaatgttg tcggttcatc caagatcaag atatctgttt
    28261 cctgagcaag cgccatagcg atccagacac gctgggtttg tccaccagac agcgttgcga
    28321 tatcccgcat acgaaatcca gctagacctg tagcatccaa cgcccactcg attgcttcat
    28381 gatcttgcgt gtccatgcca ctgaacagtt tttgataagg gtatcgtccg taagatacta
    28441 cttcttctac tgtcatacct gtagggtgtt cactagtttg tgctaataat gtaatttttt
    28501 ttgcaagttc ttttgaactg tatgtagaga tatcagagtt attgacaatg atctttcctg
    28561 atttcacagg tagtatacgg gtaaaacttt tcaataaagt agattttcca cttccatttg
    28621 gcccaatcaa actagtgatt ttcccttcag gaatcgtaac agacatgttt tcaatgattt
    28681 ttttgttttc gtagccggct gcaatgtcag ctgctcttaa tctcgtcatt tcacccatcc
    28741 tttcgctgaa aatcattctc aattatgacg ctgtttcaac atttcgtcaa gcgctacttt
    28801 catatctata gccaaatgaa gcggttgaca gcgttcgaaa tatcaggaat aataaccaat
    28861 gagaaagatt ctcaattatg ttaggaggaa agaaatgaaa ttaaaaacaa aagtattcac
    28921 attatctagc atattgctgc tggctggagt actgagcgct tgctccccct cttcagataa
    28981 agaaacagct acaactgcca attcgacgac ttcatccaat agtactcata cagtcacaga
    29041 tacacttgga cataaagtag agattccaaa cgcgcccaaa cggatcatcg gcagttactt
    29101 agaagactat ctgattgcac taggggaaaa acctatcgcc cagtggactg ttggcagtgg
    29161 ttccattcaa gattatctgc aagatgattt aaaggatgtc ccaacgattt cttatgacct
    29221 tccttatgag aaagtgttaa gctttgagcc tgatttgctg ttgatttctt cttccgctac
    29281 tgtcgaaggc gggaaatacg aacaatatag caaaatcgcg ccaacttatg ttgtaaaaaa
    29341 cggaacagat gtcacatggg aagaacaact aaaagatgtc ggcaaagctt tggcaaaaga
    29401 aaaagaggca gaaaaaatca tttccgatta tcaaaaatcc atcaagaaaa ctcgagaaga
    29461 actagcagat aaaatcgaga acaagacagc tgcagtgttg tgggtgacca acaactcagc
    29521 ctttatggtc agcaaaaatc ggtcaagtgg aagaatcgta tacgacgatc tggaatttgg
    29581 tgttcctgat ctagtggaag aggtctccaa aaaagccact tctgactggt ctgctgtttc
    29641 ctctgaaaag ctagctgaat tagatgctga ctacatcatc cttgtgaaca gtgacaaaga
    29701 tgctgccatg ttcaatgaac caagctggaa gaatcttaaa gcagtgacag acaatcatgt
    29761 actgcaattc ggacctgaat ctagttggct atataacgga cctattgcca gtcaggaaat
    29821 ggtcaatgat atcaaagaaa aactaaattg attttaacta aaaaaagaat cttcacgatt
    29881 agtcgtgaga tgcttttttt cttattaaca aactagacgc atagatttta ttcttctttt
    29941 gaagcaaccc tattttttac tctttcccat ttttgaaaac gatgggcata tagattttct
    30001 tgatccgtta tcccctcata ctcctctata agggcgaagt ctgaaaaatc aatattccca
    30061 atatacgtat cgccttcaaa cgtgctatcg atcaatgttc gccagatccg accagtgtaa
    30121 ggaagaagtt cttgaaaaat ccgactacca cccgaaacat agatatcttc tgagatcgtt
    30181 tgactatacg acaaaagttc atcgatcgaa tgcattactt ccgcattttc tttcgtgcga
    30241 aaatttggtt gcgttgtcaa cacgatgata tggcgataag gaagtgaaag atcccccatt
    30301 ccttcatagg tctttcttcc catcactaaa attcgatcca ttgtatgttc gcgaaagaaa
    30361 cgcatatcat ttggcaatcg ccatggtaac aaaccatttt tgccgatcag accattttta
    30421 tcttgtgccc acatggaaat aaacatatta tccgctcctt atgattttag atgttttgag
    30481 ataaaaaaag tctcaattct ttgaccagca aggaattgag aactttaaaa acattatttt
    30541 gtttcacggt gcaaagtaac tttgcgttcg cgtgggcaat atttttgttt ttccaaacga
    30601 tcaggattgt tacgtttgtt tttgcttgtt agatagttac gttctttaca agaagtacac
    30661 tctaaagtga tgtttacgcg catgtttttc cctcccattt ttacaagaat cctctaagat
    30721 gactctcaga cttgattatc ttaccatgat tttttctaga ttgctagtgt ttttcacgat
    30781 tttgttaagt aaaactactt gacgataaat acaacggttt tacttctcaa acaaaatctt
    30841 tttctttttg tataaatacc cttacacttg tttatataga ttatgttttt taataaaggg
    30901 ggatactaat atgccatgga acatgaagga ctttcctgca tcaatgaaaa acttagataa
    30961 attgacaaga aaaaaagcaa tcgacatcgc aaatgcatta ttagatgaag ggtaccctga
    31021 taaccgtgcg atcccaattg ccattgatca agcaaaaaaa tggtatgaag atgctagtga
    31081 atcagaaaaa cgaactttcg aaaaagaaaa aaatccaagc aagacggatg aacacgatac
    31141 gaatcctcgt gctggaaaac tgcttgattc agatgtgatc gtagaatatg aggaagaaca
    31201 atggatcgtg aaatctaaag gggcaaaaaa ggcaagcaac cattttgata caaaaaaaga
    31261 agcaatcgaa aaaggaaagc aagtagcaca aaacaaagaa tcaactttgt tcatttataa
    31321 aaaagatggg acaaaagaaa aagaaatcga ttattgaaca tatgtaaagg ggttgtgacg
    31381 aaaatcgtgt cacaaccccc ttatgttcga aacaatggtg ttcctctgcc aactccgaac
    31441 cataagtcat aatatctata aaacgggaag agctgttttt tgaaattgtt tcttatcact
    31501 cagtgttaaa cggtaggaga aaacctcttt tttattgata tttgattttc atcaaacagt
    31561 cacatagtcg attgcttctg cttgcggaat cgtttcatct acaaagtaga ctttatacca
    31621 tgtcaagaaa ctgaagattg tccagatttt tctttgctct tctttacgtc cttcgtgatg
    31681 gtcatctagc aacttcatga ttttttcttg atcaaaaaat tcttttgcaa aatctgcttc
    31741 gaataatgct ttgacttgtt cgtagccgtg ttcttctttc aaccaagctt tgattggcac
    31801 agggaatcct aatttcacac gattcgacca ttcttccgga agatgtttgt tcgcagcttg
    31861 tctgaaaata tcttttgtgt tgttttgatt caataaaaac ttacttggta tttgttgagc
    31921 caatttcatg acttcgatat ccaataaagg tactcggact tccaaagaac ttgccataga
    31981 aagtttatct gcctttaaca agatatcttt tggcatccat tgatgaagat cgacatattg
    32041 catcttattg acttcatctt tgattccttc tgttttctcg taatgaacag tcatgatttc
    32101 atccacagat ggtgctgttt gatattttgg tgttaacact tctgatgctt ctgattcttc
    32161 gaacactcga gcatgaccga taaaatattc ttttgcagga gcaagtgatt catataaatg
    32221 gatccgtcca tggaaatttt tcatttttcc gattttacgt cctaaattat agcgtgttcc
    32281 ttttggtaat tttttcaatc cttgagcaat cacacggatg aattttgaat tcgtatggaa
    32341 tccatacgtt tggtatcccg caaacagttc atccgcccct tctccagatt ggactacccg
    32401 cacactttgt gaagcaagat ttgctaaaaa gtacaaaggt acgcaagaag gatttgaatc
    32461 aggttcatct aagtgatact ggatcaaagg gaatgctttg aacgacatgt ctccgtcgat
    32521 cactgctgct gtattatcta agtccaatag ttcggttagt tttctggctt caatcgcttc
    32581 attataggtt ttatcatcaa acccgattga gaaagaatga tctggtttca atacagaggt
    32641 tacataactg gaatcaaccc cgctggataa gaaagaacca acttctacat cactgaccgt
    32701 atgtgcttca atagaagctt gaacggtttc gtcgattttt tcgatccatt cttgtcggga
    32761 atgcgtttct tttgtttcaa aatctgcatc ccaatattgc tgaatgtcta gttttccatc
    32821 tttatacgta taataatggc cttcaggcaa acgatagaca tctttgaaga atgtttcgcc
    32881 gtttaatgga gaatattgga aagtcatata aggtttcaac gcttctttgt tcaattcttt
    32941 gttaaaatca ggatgaggca gaaaactttt gatttctgaa ccaaacataa atgttccgtt
    33001 catttctgca taataatatg gtttgatccc aaagtggtct cttgcaccga ataactcatt
    33061 cttttcatta tcccaaatag caaaagcaaa cattccgcgg atcttttgca ataattctgt
    33121 tccccattct tcgtaaccat gcaaaaggac ttctgtatct gcatgtgttt gaaaaacgtg
    33181 gccagctgca atcaattctt ctctcaaagg ctggtaatta tagatttcgc cattaaaaat
    33241 aatgatcttt gtccgatctt cattgtaaat aggctgagta ccgccctcta agtcaataat
    33301 acttaatctt ctgaatccta aagcaacatg tttatcgata tattctcctg aactattagg
    33361 tccacggtga acgattcgat ccatcattgt attaataatt gtttttttgt tgtccttgtc
    33421 gttaacaaaa cctacaatac cgcacattta actttcacat cctaatataa aattactgtt
    33481 tatttcattc atatgcatct tatcatagaa taaatagcgg acgaatattt tctctcttac
    33541 aatttcgtca cgtgaaaaca aaagatgaaa gtttctgttt ttacagaaaa taaagtccca
    33601 atatctaatt taccatgttt tcttttttga ggctacctta ttctcttcgt gttaaaaaaa
    33661 tattagtgga tgctatttcc aaaaaaaaca gcaaaaaaag acacgaccta aagatcgtgt
    33721 ctaattatga ctgtatcaga aaatatgata ccaaacggat ctattcatta attaattgac
    33781 atagtttatc tgttgctctt ttatgactgg ataactgacc aattcatgac gatccaagtc
    33841 ttctttattt atatttacag cagtaatttg actgtcttca tacgtacgat aataataaat
    33901 ccctttatca acgttacagc aagaagaata aatcgtatat tcgtattttc cttctccagt
    33961 atcgcatact cctttttgtt gctcgactga gcctagtata tgaaaaaatt gactgatact
    34021 ttcagattct gaatcacctg atgcagcatt cattttagta aatgtggctt ttacaaatcg
    34081 agaaacagaa gagagatctc caggtaagcc caatcctccc atcccacgac tataaacatt
    34141 caagctgatc tggtttgaaa aatgattttc tggtgttcca tttgatagcg agcgataatt
    34201 gttcagatta aataattgat aatcaaatgg cggattattt gtcaacacac cgacaggatt
    34261 atcatataaa tgaagtccat ctttcatact ttcaattact atcgattttt ctttatcagc
    34321 taataaccag tgtaaaggtg acaagggtag tttttcacta aaatcgatat tgactagatt
    34381 gatagtagcc aataattctt tagcttcgtc tatggttgag cattgcccta gtatccatgg
    34441 aataaactca aatggtgcaa cgttatcttt tccttcttgt aattctttat aatcagcatt
    34501 gccaggaaaa ttcagtcctg ccatactcaa ccctttttca tttgtagcct cataatagag
    34561 tggatagttg gataccccag ctgctattcc tatcattgca taatgtttat ctaaattttt
    34621 tacctttcta aaatcgaaac gataatttct tggagtaata gtgactactt cattataaga
    34681 catctcataa tcaaaattcc ttccaaaata atggtctttc gttgcataag tgattgcggt
    34741 acacatcgtt ttccctccta aaacaaatcg tttttacact tttatcttac atctagtatt
    34801 tttttttatc aaaaaaaagg ccaaagaaga ttgatttact agcatttata aaaaatagta
    34861 ctcaggggga atcaaaaaat attgtaggtt aaaagtacaa caataagggg ctgtgacaaa
    34921 agtcttgtca cagcccctta ttccgaacaa acggtgttca aaagcttgca tctgcggtaa
    34981 taagcccaac caaacgaaaa aataggaaaa gctatttttg tttggccgtt cttattactt
    35041 gatgcaggac agcttcttca caacctctaa tcgttgatat aaggaaggta caggatttga
    35101 acctgcgcgc cggtattagc cggttcgccg gatttcgagt ccggtgcatt accactctgc
    35161 caaccttccg taagtactat ttttaaactt ttttcacgaa atgtcaagcc tatttctaat
    35221 agataaataa cagaaatcgc tttttttagc aaaaaagtcc attgacagtt tgcagccaac
    35281 ggacttaccg atcaaattca tactgataga acactatctg attatttcag gattatctaa
    35341 tgctaaaatc gagtcaattg tccgtttgat cagtgcaatg atttgttctt ctgttggatg
    35401 catgatgttt gatgacatca atgacgctaa gccagtcact gctacccatg taccagtatg
    35461 cagagcatcg acatattcat ccgataattg attgtatctt tcatcttctt taactatttc
    35521 acggaaataa ttgtatgaaa aatcatgcat tttctttccg tccccatgtt catcaatgta
    35581 caatgccatg taaagactat gatgcttttt tgcaaagtag atatagttca aaccgagatc
    35641 aatgatttta tcaccagtac gttcaatagg gaaaacatct tcttttagtt gttcaaagat
    35701 agaatccagc aacgtatttt ttaaatcttc catgtttttg aattccagat aaatcggctg
    35761 tgtcgaaatc cccatctttt tagcaatatt tctcgcagta aatcctttaa acccttcctt
    35821 tgcaacaaca tcataagcag cttttagaat ttgatccttg gtatagactt ttcttctcat
    35881 taaaactccc ccacttcttt actgcagtat atgttgtttt attgtaccct atttttagca
    35941 ttttttgcaa acattttact caaaacgtta tattttaaat ttttttcagt cttctttaac
    36001 ttttttttgc aaaattaaaa aaatttggtt aaattcaatg ggttccctta catttttagt
    36061 tttgacctaa atgatagttg tccaaatctt tcatttcaag ttatagttaa agaaaatgaa
    36121 aaagaaggtg tgtaatggaa aacgaaactg acgttattta tattcatcct caaaaacgaa
    36181 tcgtttcaca gaaaagaaag tatttttatc ttggttttac aggtgttttt tttcttttta
    36241 ttggcttgct ttctaatact cctactgata attggagtgg attgctaact attcttacct
    36301 cgccaagcaa tcttttgaca gattattttg ctttaggtgt tttcggaagc gcattcataa
    36361 atgtagggat tttgacctta ctttctgtgc ttttagctta tcggcacaag gtgattttaa
    36421 acggtccctt atttgcttcg attctaacag ttaccggttt ctcttttttt ggcaaaaacc
    36481 tttataattc tatctcaatc attttaggtg tgtatctata cgctgtattt gtcaataaac
    36541 ctttttctca gtacatcatg atcggactgt ttggtagcgt attatcaccg gtcgttagtt
    36601 atatcacttt tggtatgcgt tttcctttac tgataggtat tttattgggg aatttagctg
    36661 gcatagcaat tggtatactt cttccgccct tagctgctca aactttagtt ttccatcgtg
    36721 gattcacact ttataatatt ggattcacct ctgggttgat tgcgatgact tttactgctg
    36781 ttttgaggtt attcagctat tcaatcgtcg aaaatacatt ggttttcaat gaatatcact
    36841 ttcctctcat ttggattatt tttggctttt tttcgctgac agtcggaatt ggtttctatt
    36901 ataattcctt tcggctttcg gggattcgag aaattttcga ttcatctgga aaattgacaa
    36961 ctgatttcat tgctaattct ggtattggtg ctactttgat caacatgggc ttagttggtc
    37021 tgatgctgtc ttcttatgtt ttgctggttg gcggtcagtt aaatggacct gtcatcggtg
    37081 ctattttatc tgcggtgggt ttcagtgctt ttggttgtca tttaaaaaac agttttccga
    37141 tactagtcgg aatatttatt gcttctttgt ttggaacttt tcatgagatc accagtactg
    37201 gcatgcttgt tgcagctgtg tttgggacag gtcttgcgcc cataagcggt ttttacggca
    37261 gtttttacgg tgtaatagct ggtgtgctac atattgctct tgtccataac gtcagcactt
    37321 tgcacggagg actaaatcta tataacagtg gtttttcaac tggtttcgtt gcaggtatcc
    37381 ttgtaccgat actagataac tttaccgctg tcagaaagga aaaaaagaca cttggaaaaa
    37441 gaattatcaa aaagaatcat cgatgagctt cttacttatt tatattccca tgaaataact
    37501 gaaatacgta ttggagtaga atttgctgag gaagggcttt tcatagagat acagggtacg
    37561 gcagaaactc agccagaaga tttagatgta cttgctgatt tgctgaacaa tcctcgggac
    37621 cctagtatgg aaatctacta tcaagaacta ttaggtaatc cccgacatga tcgggaagat
    37681 tatcatctgt taggtagttt gattgatgac gtggaaatca tgtatgccca gccgattatc
    37741 aatattaaga tctttcgacg tgcttctcat tcctattatt aaccctattt tctatttata
    37801 aaagactgaa tcaaaaaagc agctcctgaa agttcgatta aaatctactt tcaggagctg
    37861 ttcaagcttg acattattga ttttccaatc cttcatacac ttcatctgtt acaggttcta
    37921 aaaactctga attaccagca gtgatcgcca aatgttcaaa ccaactgtct tttgtcgcac
    37981 catgccaatg tttaatccct ttgtgcgtta cgacgacatc tccagccatt aatcgtcttg
    38041 ctggctttcc ttcttcctga taccagcctt ctcctccggt cacaagtaag atttgatagc
    38101 cgtctgtatg gacatgccag tgattgcgac aacctggttc aaaagttaca ttacctacac
    38161 caacagaaac ttcgggatca ttcactaaca tattcagata gctttgcccg gtaaatactt
    38221 gagcgtaagc ttcgtttttt tcacctgctg gaaaaatcac gccattgtgt atttcttcgt
    38281 gttttgtcat catttacgcc tctttcttat cttcgctatt ttcttctaaa tagatttctt
    38341 ttgctaaact aaaagctgac caagcttttg gccattctac ataaaaagct aaatgggtaa
    38401 tgatttctgc tatttcttca ctagtaatgc cattttgctt gccaatcttc agatgagctg
    38461 gcaattgttc aaaatttcct cctgaaatta atgcagatat ggtgatgata ctacgctgat
    38521 gagcagataa ttcttctctg gaccatactt gtccaaataa aacatcatca tttaattctg
    38581 caaattttgg tgcaaaatct cctaattgat ctcgtcctgc ggtttgtttt tctgccattt
    38641 ctcttattct cccttcacga ctgttaggtg attcaactat ggttctaagg caactttatt
    38701 ttctattgtt tcttcgcatt tcattttaaa tgtagtcttt cagcctgtcc aatgcttagt
    38761 ttattcagtc gatatgcttt ttaagcatag ttgctaacgc tttttttatt ttaaaaaaaa
    38821 caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt tatacttatg
    38881 atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt tagttgcttt
    38941 tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg aatattgctg
    39001 cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca atcgcgctga
    39061 cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga ggattagtag
    39121 gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg caagtagtta
    39181 ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt ggcccttatg
    39241 ctggatttat ctataactat attggaatct gcattggttc gattatcatt tttctgctag
    39301 ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca tataacaaat
    39361 atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta gctatttttc
    39421 ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac atgtcagtga
    39481 aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta tatagtatgg
    39541 ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta ttttgtaaaa
    39601 ttagtcattg attgacaatg ttaa
//
LOCUS       DAZUMM010000017        38902 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000017 DAZUMM010000000
VERSION     DAZUMM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.017"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..300)
                     /locus_tag="ACG8E6_000821"
     CDS             complement(76..300)
                     /locus_tag="ACG8E6_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061263.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(320..742)
                     /locus_tag="ACG8E6_000822"
     CDS             complement(320..742)
                     /locus_tag="ACG8E6_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5061264.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(885..1595)
                     /locus_tag="ACG8E6_000823"
     CDS             complement(885..1595)
                     /locus_tag="ACG8E6_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFS5061265.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(1613..2110)
                     /locus_tag="ACG8E6_000824"
     CDS             complement(1613..2110)
                     /locus_tag="ACG8E6_000824"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5061266.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(2147..2599)
                     /locus_tag="ACG8E6_000825"
     CDS             complement(2147..2599)
                     /locus_tag="ACG8E6_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5061267.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(2734..3339)
                     /locus_tag="ACG8E6_000826"
     CDS             complement(2734..3339)
                     /locus_tag="ACG8E6_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS5061268.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(3789..4409)
                     /locus_tag="ACG8E6_000827"
     CDS             complement(3789..4409)
                     /locus_tag="ACG8E6_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5061269.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(4411..5280)
                     /locus_tag="ACG8E6_000828"
     CDS             complement(4411..5280)
                     /locus_tag="ACG8E6_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS5061270.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(5303..5710)
                     /locus_tag="ACG8E6_000829"
     CDS             complement(5303..5710)
                     /locus_tag="ACG8E6_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS5061271.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(5729..7018)
                     /locus_tag="ACG8E6_000830"
     CDS             complement(5729..7018)
                     /locus_tag="ACG8E6_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5061272.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGDFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(7045..7347)
                     /locus_tag="ACG8E6_000831"
     CDS             complement(7045..7347)
                     /locus_tag="ACG8E6_000831"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5061273.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(7372..9465)
                     /locus_tag="ACG8E6_000832"
     CDS             complement(7372..9465)
                     /locus_tag="ACG8E6_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5061274.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(9954..10718)
                     /locus_tag="ACG8E6_000833"
     CDS             complement(9954..10718)
                     /locus_tag="ACG8E6_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFS5061275.1"
                     /translation="MKKINYLAIILLVGLCLFALPPQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(10829..11698)
                     /gene="mdcB"
                     /locus_tag="ACG8E6_000834"
     CDS             complement(10829..11698)
                     /gene="mdcB"
                     /locus_tag="ACG8E6_000834"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFS5061276.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(11723..12799)
                     /locus_tag="ACG8E6_000835"
     CDS             complement(11723..12799)
                     /locus_tag="ACG8E6_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS5061277.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(12823..13416)
                     /locus_tag="ACG8E6_000836"
     CDS             complement(12823..13416)
                     /locus_tag="ACG8E6_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFS5061278.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(13420..15096)
                     /gene="mdcD"
                     /locus_tag="ACG8E6_000837"
     CDS             complement(13420..15096)
                     /gene="mdcD"
                     /locus_tag="ACG8E6_000837"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFS5061279.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(15086..15385)
                     /locus_tag="ACG8E6_000838"
     CDS             complement(15086..15385)
                     /locus_tag="ACG8E6_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFS5061280.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(15487..17133)
                     /gene="mdcA"
                     /locus_tag="ACG8E6_000839"
     CDS             complement(15487..17133)
                     /gene="mdcA"
                     /locus_tag="ACG8E6_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFS5061281.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(17140..18051)
                     /locus_tag="ACG8E6_000840"
     CDS             complement(17140..18051)
                     /locus_tag="ACG8E6_000840"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS5061282.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            18171..19028
                     /locus_tag="ACG8E6_000841"
     CDS             18171..19028
                     /locus_tag="ACG8E6_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5061283.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(19215..19577)
                     /locus_tag="ACG8E6_000842"
     CDS             complement(19215..19577)
                     /locus_tag="ACG8E6_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFS5061284.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(19766..19948)
                     /locus_tag="ACG8E6_000843"
     CDS             complement(19766..19948)
                     /locus_tag="ACG8E6_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061285.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            19923..21362
                     /locus_tag="ACG8E6_000844"
     CDS             19923..21362
                     /locus_tag="ACG8E6_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5061286.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(21369..23921)
                     /locus_tag="ACG8E6_000845"
     CDS             complement(21369..23921)
                     /locus_tag="ACG8E6_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFS5061287.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(24035..25366)
                     /locus_tag="ACG8E6_000846"
     CDS             complement(24035..25366)
                     /locus_tag="ACG8E6_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5061288.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(25332..26810)
                     /locus_tag="ACG8E6_000847"
     CDS             complement(25332..26810)
                     /locus_tag="ACG8E6_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5061289.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(27115..28950)
                     /gene="tdc"
                     /locus_tag="ACG8E6_000848"
     CDS             complement(27115..28950)
                     /gene="tdc"
                     /locus_tag="ACG8E6_000848"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS5061290.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            29420..30847
                     /locus_tag="ACG8E6_000849"
     CDS             29420..30847
                     /locus_tag="ACG8E6_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5061291.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(30939..31304)
                     /locus_tag="ACG8E6_000850"
     CDS             complement(30939..31304)
                     /locus_tag="ACG8E6_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS5061292.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(31301..31666)
                     /locus_tag="ACG8E6_000851"
     CDS             complement(31301..31666)
                     /locus_tag="ACG8E6_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS5061293.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(31858..32106)
                     /locus_tag="ACG8E6_000852"
     CDS             complement(31858..32106)
                     /locus_tag="ACG8E6_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061294.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            32105..32233
                     /locus_tag="ACG8E6_000853"
     CDS             32105..32233
                     /locus_tag="ACG8E6_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFS5061295.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            32290..33984
                     /locus_tag="ACG8E6_000854"
     CDS             32290..33984
                     /locus_tag="ACG8E6_000854"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFS5061296.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            34057..34257
                     /locus_tag="ACG8E6_000855"
     CDS             34057..34257
                     /locus_tag="ACG8E6_000855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061297.1"
                     /translation="MIWLDLLPQMQAFEHRLPRGCEKAALHQVIRAAKLKQLLMFLTW
                     LDLLPQMQAFEHRLPRGYEKVV"
     gene            34635..35768
                     /locus_tag="ACG8E6_000856"
     CDS             34635..35768
                     /locus_tag="ACG8E6_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFS5061298.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQNGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            35799..36119
                     /locus_tag="ACG8E6_000857"
     CDS             35799..36119
                     /locus_tag="ACG8E6_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFS5061299.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(36148..36612)
                     /locus_tag="ACG8E6_000858"
     CDS             complement(36148..36612)
                     /locus_tag="ACG8E6_000858"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5061300.1"
                     /translation="MVTYNWIKLREHPELVKQSAKWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(36741..37990)
                     /locus_tag="ACG8E6_000859"
                     /pseudo
     CDS             complement(36741..37990)
                     /locus_tag="ACG8E6_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(38019..38336)
                     /locus_tag="ACG8E6_000860"
     CDS             complement(38019..38336)
                     /locus_tag="ACG8E6_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5061301.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(38362..38682)
                     /locus_tag="ACG8E6_000861"
     CDS             complement(38362..38682)
                     /locus_tag="ACG8E6_000861"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5061302.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDIINMADYGMMNGEKVLDQAEK
                     LINK"
BASE COUNT        11532 a         8102 c         6677 g        12591 t
ORIGIN      
        1 ttcatgaaca ttcactttgg attacaagaa tttctttatt gtgttaataa agtttcttca
       61 ttctagtctc aaaaatcaat agtgtgattt gattttacca tttcgtttct gttcttcttt
      121 gatgaattct tttgtatcga caggattcag attatttagt ccgccaaaac ctgtatcgac
      181 catttttccc cagccttttg tcttcttttt tcctgatttg agattctttg ctcgaagctt
      241 ctcactatgt tttgctcgcc gatacttcaa ataaccttct aatcttccca ttagatccac
      301 tgtttttcct cctttttatt caatcagacg actcagcttt tcctataaca aaataattgc
      361 cttcagggtc agaaaaattg aaagtcaaga tgccattgtt atctgtgatt tctcctggtt
      421 cttccatgtt ctcgtagata gtttcaaatt catccgtaaa aaatactaaa gaaggcggtg
      481 gtcctaatac ctctggtgaa aacttctgca caaatacttt tgggaatacg cctaattcta
      541 attcttttga gatgctgagt acaatactct taaattcttc tggcaattcg attgtttcag
      601 aaatttctgc accaattttt tctacaaaga atcggcaaat catctctacg tcattgacat
      661 aaagcatcac acgtgttttt tgtatcatca tttgaccctt ctttctattt gattttcttt
      721 cctgtctata aactgattcc attatatcac gaaagcgcaa acaccttgtg tttgatacta
      781 ttcaagaaaa ataaagcaat agtttctagg attgctttcc tccacaaact ttttttacaa
      841 aaaaaaaaac ggcaatgaat gccgctcttc ttatttcgat tcttttataa ttctgcttct
      901 gattcatctt tggttagtgt gtgtctaaca ctagttacgc caaatgtttg atcgattttt
      961 tccttcaacg cttcgattgc ctgtgcgcca cgtgccgctt gttcagcatt gatggattca
     1021 atgaccagca ccgctgcttg tgtttcttcc gtcagcatcg ttctttgggc ttcacgccag
     1081 ttgagacagc gacagatcgc tccttgttca tcatagtaga tgatttcttc cggaagtgcc
     1141 ggagcatcgg aatctgcacc taaaggcagg aatgattcgc caccttgggc ttttcctaaa
     1201 tgcagatttc ctgcgatctt ttcgatatct tctccgccgc aaggtacgcc aaaagacaag
     1261 gaaatactat tatagatatc tacaagcgga ttgattggga aaaattcccg ttcttgattt
     1321 gcacgtttca gtaaagcttc aatagaagaa cgtgcacctt ttttagtctt gaatgtggta
     1381 aatgcctgac gccactgagc aatgacttcg ttttggctga atgtttcttc tgtaaggtaa
     1441 tttcttgctt cttttgctgc ctcattcaat aattgatgat acgtttcttc actttctttt
     1501 gttacgtggt tatcgatacc atttacggat aagatttcga tggttgcttc aggaaatact
     1561 tcccagaaat tggtatccac tattactttt ttcatctgtt cacacctctt gattagaata
     1621 attgcttgat gaatagatag tctgtttggg gatcatctcc catataaaaa acatgttcgc
     1681 tgtatttgat aaatcctttt ttacgataaa aagcgagcgc aacatcgttg tgttcccaaa
     1741 caccgagcca aatggctgtt ttttgctttt cttttgcttt ttcaatagct gtatctagta
     1801 aaaatgtacc gataccttga tgtttaaatt tttttcgtac atagattcgc tcgatttcta
     1861 aaaaatgatc agccatttcc tcagattgac tcgttcctgt atttagtttc aggtaacccg
     1921 caggctcttg atcgatactc actaggaaaa aatcggtttc tttatctttt agctcttctg
     1981 taagtttttc tagtgcataa gcttgtgtca agtaatcagc taaatgctcc ggtgaattat
     2041 cttttccaaa tgtatctgta aatgtttcga tactaatttg ttgtagtaag tctacatctg
     2101 aaggcgtcat ttttcgaatt tcgtatttat tcacggcttc ttcctttcaa tagatccttt
     2161 tattgccttt tttgacaaaa ttccaatctt cgctaacatt cttgcatact ttatttaaaa
     2221 gatattccaa ttgttttgct tcttcgtcag aaagaccatt taatgcgact gcattggagt
     2281 agttgttttc tcgaatgatg aaaggataga tttctgctcc ttttttagtc ggatagagtt
     2341 tcttgatttt tttattttct ttatcttcca aacgttcgat catcccattg gattctaatt
     2401 tcttgattgc tcgggcagtc gttgtacgat ctactttgat catttctgct agtttttctt
     2461 ggatgattcc aggattttcg caaatacgta ctaaataaag atactgtcct cttgttaaat
     2521 cgacttcttt gaattcgata ttgctaatag aatctagcgc tcgggcaatc attccgatgt
     2581 ctcgaaggat ttcagccatt tcatcccacc tttattttgt ttgtaagact agttcacctc
     2641 tttttgttgc aaatgcaact attttcaata aaaaaatcaa gttctttttt aataactagt
     2701 aaagtatatt cttttcttct tttacgaaaa agctcaatca caatagatct ggtttttgtt
     2761 ttctaaaata agtaactgtt tcttcgttgt tcgcttttct gatagctttg gcaactcttc
     2821 tatactaaaa aatcccatgt tggaagtttc attattctca ataaacttgg cctcttcacc
     2881 gtggatcgca caagcaaaaa caagtttata atactgaaaa agctgtggaa tatctttttg
     2941 tttattcgta tcaaaaactg ctcgcaactg agtagtttct actgttaatc ctgtttcttc
     3001 atagacttct ttacgcacat tttcttcagg agacaatcct atttcagcaa atccaccagg
     3061 aagtgaccat tctttcgtcc gtttatcttc gattagtagg accttgccat cttttttgat
     3121 ataggcacga acatctactt ttggtgttgg atgtccttct ccaatcgttg tgagcgcttt
     3181 tatttcttcg aaagtttctt ttcctatttc actgatcagt tccaatgaaa tcgtccggat
     3241 ctcctcaaaa cgctctttat caaatacatc ttttccataa tatagaccag cttgagaaag
     3301 tgcgagtagt cttttgtatt gagccaactt cttgttcatt ctcttccctt cttttttagc
     3361 aaatgtatga ctatgtaatc aagtatagaa gactggttta attacggcaa ataaatataa
     3421 tgaaatctgc aataaataaa agaatgttaa aaaactcgag aacaaaagac attcgacttc
     3481 tctggggctg tgaataaatg gcgttcaaaa gcttgcatct gcggtaataa gccccaccaa
     3541 acgaaaaata tgacgagcta ttcttgtctg gctgttctta ttaattgagg caggacagct
     3601 ttttcacaac ctccccacta aacggtgttc tcctaccaac tcctcggtca caagtcacaa
     3661 tattcatcaa acatgagaag ctgttttttt gaaattgctc cttgtgactc ggagctgaac
     3721 gacaggctca cgacctctcg gtaaacagcg ttcaaaagcc aatttttcga tattaagcca
     3781 tttctttgtt acttattgtc attgatttct ttttctaatt tatccaatag tggagtgtaa
     3841 tccacttgag tatagtctgt tacatcaaat tcatatagtg ttcctaactg gaatttgtca
     3901 tattttcggt cggcgatgat ctttttgaat gcttctttcg taatatgatt ggtccctttc
     3961 gttcgatctt ccaatacgtc accatagtga taatgatcca tgatatagct caaatgacga
     4021 tctgattcac gatcacgttt gtaccgacgt tgccataaca cttcaaagtc tgctgttagg
     4081 cgaatcgtga tcacctgata tccgtatcta tctgctaatt cttggagtcg tgctttttgc
     4141 ttatcactaa aaggatactc ggacacaatc actttctttc ctgcttccat atacaaatca
     4201 agtgctttgt aatagaaagg ataaactttc gtcctttctt gttccgcccg ttcttccaaa
     4261 ttgttaaacc cgatggattc tgcatagtat tccttgattt catcgagcgt gatggcatac
     4321 atatctggaa atcgttcccg tagtttattc ataagatatg tttttcctgt accaggacta
     4381 ccagccagaa gaatgaaata ctttttcatc tcatttattt cctaagttaa ttggactttc
     4441 caagttcata aacagtccaa tcttatgctt gacaatttct tttactgctt catttgctgg
     4501 aatcacatta tggcgaagag acttgttgat ttctgtttca ctaaaggttt ctttagctac
     4561 tttcgtccag ttgactaaaa gctccgttcc aacattgaat ttgcggatcc cgttattgat
     4621 caattcagga taatcttctt ctttcacacc tgttcctcca tggataacta gggggacatc
     4681 aactactgca tcgatttctt tcagcaaggg aatgtttact tctgtttttg atttgaattg
     4741 tccatgattc gtaccgatag caatagctaa tgcatccacg cctgtagctt ctacaaattt
     4801 cactgcgtct tccggtcttg tatatacctt gtcattttca gcgacatgga ttccttcttc
     4861 tgtaccgccg attgttccaa gttctccttc tacggaaaca ccgtgcgcat gggcatattc
     4921 tactactgct tttgtcttca ggatattttc tttgaaaggc agatgggacc catcatacat
     4981 gacagaagta tatccgctgt cgattgcttc tttgatatca tcaaagtctc gggcgtggtc
     5041 taagtgcaag actgcatcaa ccatttcttc ttctgcaata gctgtcatta cattgactaa
     5101 cacatgcatg ccgatatatt tagcggtatc gacacttgtt tgaataatga tcggcgaacc
     5161 catttctttt gctgcgccta gcatagcagg aagcatttct aggttatgcg tattaaaagc
     5221 tccaactgta aaattcaact cttctgctgt ttttgttact tctttcaatg ttgtatacat
     5281 cttttattgc caccttatct ttttatttgt ttcctttgat cgttacttgt tttgcaattt
     5341 gtcgcatctc atccattttg cccatgtagt aatcgatccc ttgatcatca ccagcacgag
     5401 cagcatctgc acgcttctgg ttatagaggc tcatcagttt tttataccca tctccgatcg
     5461 tctgtttatt ttccaaactc ttttctaaat attggatggc ttgatcgttc acatacaatt
     5521 cagaaaattt aagtccaact tgttcataaa gtttcgctaa tttatgctta tcttgggtag
     5581 tggaaatctc ttgtttcaat tgtgtaatct ctgattgaat cttttcggtc gcttctggcg
     5641 atagggcttt ttcttctttt gccagaactt ttttcggttc tttttctttt ttcttaaaaa
     5701 atccaaacat cattttccct actttcaatt aaaatgcttt caaaactggg cttaagatcc
     5761 aggcatataa gagtgctgcg gctactgtat ctacatcggt tgctgtagca ttgatgaatc
     5821 ccatcgaatt aaagatcgtt actagcaaag cgggcaataa tgtaataaag aaaccatgag
     5881 caataccgcc aatgatcgct cctcggcgtc cgccaactgc gttgccaaat atccctgctg
     5941 tccctccggc aaagaaattg gttagcatac caggtaaaat catcgctaag ccgaacatgg
     6001 gcaaagtaaa catggcaata atcgtaccaa tcgttgtcgt aatgaaacct aaaatgacag
     6061 catttgggct ataaggaaag aaaactggac aatctaaggc agggatcgca tcaggaacta
     6121 atttcatagc tatccctcga aatgccggca cgatttcacc taacagtaga cgtacaccag
     6181 ctagcaatac atacacaccc actacgaatt ggatggcttg taaaaaggca aacatgatat
     6241 agttttgatc tcctgataat tcagaagcga atggttcgcc tgcaaataaa acagtgatga
     6301 tatatagcgg gaccatcacg accatgacag acaaataagt atcttgcaga aattcgaaat
     6361 gtgtcggcaa cttgatatct tcgatcgact ttttattttc gcctttctca ccaaaaagct
     6421 tagcaacacc ggcttcaaat aagtacccga tcgtacaaaa atgacctaaa gcaatatcat
     6481 ttgatccagt gattttacga atgatcggtt gcgctactgc cggcatagca atggcgaaac
     6541 aagctccgat aaaatcaccg accaagatca atacgatccc tcgcagaccg gcaaaatagc
     6601 caaagactgt cgtcatcgtt gccatccata aaattgcttg accagttaaa aaaatgtact
     6661 tccattttgt gaaacgagcc aaaataatat tgaatacaaa aatggctaag aacgtcagtg
     6721 caatatctct acctaaaccc aaatcattca ttgcttgacc attgatagat tcaatagaag
     6781 ggatgatccc ttgcatatga aatccttcag taaagatttt cccgaaataa gttaaacttc
     6841 caacgatgat gctcgaaccg gcactcaaca cttgaaatcc taggagtgtc tttaacgtgc
     6901 cagagatgac ttgtcctgct gatttcttct gcagtagtag acccaacata gcaatcaatg
     6961 caatggtgat cgatgcctga gtcaaaatat tttcgatgat aaaattgata acgcccatgt
     7021 ttttaccctc ttttcttttt gtatttagat aactccctta tcttgcagaa ctggggtgat
     7081 tttttcttca atttcatttt ttgaaacaat atttttcaaa tagatcatcg ccgttttagg
     7141 atcaactgcg aacttttcga actgcgaacg aaaattttct gctgtgatga tcacatctgg
     7201 tttcatggaa actgcggaag aaatatcaca atggtccagt ttggcttcta ctcctaattt
     7261 gttcaaaaca tcctcagcag acatctgtgc tgcaaaacta cttcctaaac ctgcaccgca
     7321 tacgaataaa atgttcaatt tgttcatctt tgttctcccc cgtatcattt attatttctc
     7381 aactactgaa aaaagcagtt gtttgaaatc ttcgatattt gtgcattcaa tcaatcgatc
     7441 caacttctct tcgttgttga tcaattcaat tagttctttc atgatgttca gatgcgagta
     7501 ggagtctact gctgccaaac aaaaaataat ctttactgga tccatttcag gattaccgaa
     7561 atcgattggt tgctcaatcg tagctacgct gactcctaat ttattgaccc catcttctgg
     7621 tcgggcatgg gctagtgcca tgtgtttgcc aatcacgata tacggtccgt aatcattcac
     7681 tgcacgaatc atcgcttcga catagcgctc ttcgacaatg ttttctttaa ccaatggtct
     7741 agcagctagt tcaatcgatt cctcccaagt agtggctttt tctttgatca tgatattgct
     7801 gtccgtcaaa atatctttta acataggctg gatctccctt ttgttgattt ctaaattgtt
     7861 tgtttcgaat aatttttcta acttgccata gatgattcga tctactcggc caccactttc
     7921 tttcatgaca tctattattt gagtgaataa ctccgtcgat tcatgactgt taggctctaa
     7981 ccgctgtagt tcttcatttc ttttcaaaaa gtcttctata accaagatat tcgtctcagt
     8041 taaaatcgga tcgacaacta aaagcggctt atttcgataa ttcaattgaa aggtgctgaa
     8101 aaccagattg acatccaatt tatctactaa ttcgatttct tttgaactta aaactgcagt
     8161 aatctcgatc tgaggaaact tttctttcag attctctgct aacagatttc cagtagccat
     8221 cccatggtta cagacaacga ctgatttata aacataattc aagtctcgct tgattacact
     8281 ggcgatcgtc gaaaagtgga tgacaagaaa agctatctcg tcctccagta tcttgccacc
     8341 aattacttct tcgatcgtag gaacaaaatg agtgactgcg cggtagattg ggaaataatt
     8401 tgttcggatg ttttctttca atggattcat gacttggata tgattcttga ttcttacaat
     8461 cagtgcagcc atgtgcttat agagattttc gaataaagct tcttccttta gatggaaagg
     8521 tacgtgtgtc tcgttctcta caaattggat caattggatc gttagtaatt gagcttgtac
     8581 ccactcgatt gaattggaga tatctctagc attgaacgtc tcaatagtga aacgaagata
     8641 atttctttct acttgcggga cattgtccaa ttgaaattcc tcgatcatgt ggtcaataaa
     8701 tgcagaaaag ctactgtctt ttatttctgt atttttccaa ttaatggcag agataaattc
     8761 atgtttcctg attcgctgga tccagatcaa tgtaaaaagc aggatttgtt ttttataaat
     8821 atcatcttct tgtttcaaca acttattgga atagatctcc tgtatttttt tgatttccac
     8881 caaaggaaaa taatggagaa agattttttg agccggtgtt aattgtgcat cttgattcat
     8941 aaagaagtcg gtcattccca gattctgatt gataaaatca tagatcatcg tacggataga
     9001 tcgttctgaa cctttataaa ccagtccttg ctttccataa ctgacgatct ctatcccata
     9061 actgaccagt gttgctcgta accgtctcat atcttcatct aatgtacttt tagagatttg
     9121 aaattcttct tcttttttgt ttagatggat cgggattcgg ctgtaagcaa cggctaaaac
     9181 tagatcaaaa atacgttcgc gccgattcaa aaagccgtct ttcacatctt ttatttcagt
     9241 ttctaatcga gcttgctttt ccttttccag cttcaacaaa aaacctttgt tgcgaattgt
     9301 acggatctcc ggaagctttt tactgctcaa ccagatattc gcttcaagaa cttcctgacg
     9361 gatcgtccgc tgactgacat tgaactcact tgctagatgt tttgctttta ctggatattg
     9421 ggctgtcatg attctgtgaa gtatttcaat tgttcttgtt ttcatagaaa tgatctcctt
     9481 ctgatttcaa gtatatggtt ccgcttacat aataaaaagt agatatttct gccacttgaa
     9541 gtggaaagaa agtaagcaaa gcaaacaaaa aataagccat atgctttttc atctcctcaa
     9601 cgaaaaagac atacgcctat tattacaaaa attttgccca tctatacaaa aaagaggttg
     9661 tgaaaaagct gtcctgcatc aagtaataag aacaactaaa ctaaaatagt gcttcctatt
     9721 tttcgtttgg ttgttcttat tatcgcagat gcaagctttt aaacaccatt tagtggagag
     9781 gttgtgacag agatgttcag ccttgagcga taagaagcga ttttgaaaaa tcgcttccca
     9841 tattttacaa aatcgtgcct tattgccgat aggctatctc tggcacaccg tttattcaga
     9901 aaaaggagct gcgacaaggc ttttgtcaca actccgttca taaatatttc ctgtcaatcc
     9961 agcataatcg gctgaataaa atcggcttgc catctttttt ctttctcaga aaaatcaaaa
    10021 taccatatct gagaaaaata ctgtcgttgt gtcttacttc ctgagatcaa tcgcccatct
    10081 tttcgatcga ctgaataatc acagcatctg aaatcaattc ggatggcaaa actattttcg
    10141 tggatctgtc gataatttcc tacttcctga atagttattt ttttagtatg atttcttaca
    10201 ccgtctgctt tgtttttttg cagaactttc tgatgttcgg caaacagctt gtctgtataa
    10261 cagcttctgg ctttttcagg agtttcttct tcccaagcgg attggatctt catgaatatc
    10321 ttttggattt ccagaaactg tgctttcttt tctttagccg ttcctgacat gcgggacaat
    10381 atctccgctt tcagcctttt cttttgtctt ttattcttga cgatagaaaa agcagaaacc
    10441 cctcctactg acaaaacagc taatgcaaga aatcccgttt gctcataact agaaggacct
    10501 gtacctggac cataataccc ataagaatct ctgccgtaag aacgactgcc aaaataactg
    10561 cccccatatg aacgaccacc aaatgaacct ctgcccgaac ttcgaccgct agatgtacct
    10621 ccaccatgac cacccgcgac agcgaatcct tggggcggca gagcaaacaa acataggccc
    10681 actaacaaaa tgattgctaa ataattgatt tttttcattg tcttcctcgt tatcttttat
    10741 tacaaaaaac tatttttatg atagccttgc taaaaggaag agtcaataac cagatgcata
    10801 aactgttttt ttagcaattt tgtcttattt agtcattgag taacatcgga caaatgtatg
    10861 tgtcggcagt ccataattcc tctatcttca gcatgaacaa gcttgcagac aagagatctg
    10921 cgcttcctcc aggagagatt tggttttcct gacaaaagtg caacagtgct gtaaatggac
    10981 gatttggtaa agcactttga ctagcagcat gagcaagttt ttgcatttcc actaacgttt
    11041 tctgatcact gcgatgcaat acgcatgtgt catctagcga actcatcaaa actaacaaca
    11101 tatgcatttg tttctgtaca tatgaatcat tatctagctc cttagctgct tgaattgcaa
    11161 gcctgatatg cggataccct aatatcgctt cttcatatgc accatttacg ccatattttt
    11221 gcttcaccct atgaccatga gtggtttttt tatgacgata ttttgtatca ggaaaagcag
    11281 caagcttgct gacatcatca agtattttcg ataaagaaaa aggctgttcc atactgattt
    11341 tttgtgcaat gacactgatc aatagcccca ttgcccaaat cgctccttta tgggtattga
    11401 tgccatttgt agcaatcagc attgcctttt ccgcttttcg cccaatctca gcaattcttt
    11461 ctcttaactc ctgatcaata gcatgatgcc atgctatttg agccatttct tcaaaaaaag
    11521 gagttagcgt cttagcagac ttaagaaata aagcatagtc catatcggaa tgtgcacccg
    11581 catccagtgc atccactaat ccaggttttg gacttaatgt tacttctgcg atcaatgctt
    11641 cctctaccaa cgcagcgagt tgctgagtca ctgctttttt tcgttcgtca tttatcatga
    11701 cactcactcc ttcttcctac tattacttgc tgaaaccttt tttgatagct acccaaatga
    11761 tatgaagtaa ccagcttaaa gcaaatgcac aacttgccgt cattagataa gctacaagaa
    11821 tcatgactcc tgaaggtaac tgcagccaac tatctttaaa caataataac agtacttgga
    11881 aaacaaatac attggctaaa tacgtacgat aagcataaac agacaaccat ttgaacaatg
    11941 gcaattttgg cgaatcgttt acaatcattt tggaagcgcc tatgaagaca gcaaagatga
    12001 tgactaaact gtataaactt tggatcgtat tcaagtaagg tgcattcgca aagctcaagt
    12061 ctcctggatg tgcgagcaat gaacggacag aaaagaacgc gataactaag gcaacaggta
    12121 agaaaagata acgtactctg tccaaatatt gatagacttt ttcgtgataa acaaatgcgg
    12181 ctaccccata aatcatgaaa ctagcaacaa accgatctaa taagtaccag ctgtcatgat
    12241 aaggcccagt caatacttgt gtttgataga acagatacca tgcgacatat aaaatcgttg
    12301 aaccaatcaa tactggccat acatattttt tattagcaaa tactttgtaa ccgagccaaa
    12361 caaaaaacgg catgaacaat tggatctgca gcatcataac ggtataccat aaatgaggcg
    12421 ctccatcacc tgtgacatat ttcagtaaaa agcttgcagt atccgtatac ggagtttgtt
    12481 gttggactgc tggaaatagc caaagataag ccgtcgtcca tagaagatac ggaacgacta
    12541 gttcaaaaaa cttttctttc aaaaattccc aataagacaa atgctgactt gtttttacca
    12601 tattataaac aattgcaaaa ataaacattg gtgctgtgta tttaacgatg acgtaaaagg
    12661 taccaagaaa agctgtattt gttgcgctgt ggctgtttgc caaagcaaag ctcaatatcg
    12721 tttgcatcat aacagccgtt acagctgata ctttctggaa gtcacttacg ttgattttga
    12781 tttttttgtt ttcaaacatc tctttctctc tctttttctt tattaccaaa gttctttggt
    12841 taatttaggt ccttcgactg ttttcaatag aatttttttc tctggctctc tcaaatactc
    12901 ttttagtgaa aaaccgcctt gccgagtcac tacttgggta tcgaattgct caaaaaaagc
    12961 aggatgcgat tgaattacct gaattggcaa ttcaatcggt gaatttgcgt acagcagcaa
    13021 atcaaaatca ctggtttctt ttactgccgg aatcccagtc gataattcaa atcctaagct
    13081 gccgccgatt ccccatttac atcctgataa tatgtttcta gcagcatgaa attgctgata
    13141 aacaggataa tccatgattt tctcttgtct atacgattct tgttcgatga tgtcccacgg
    13201 atgaatgact tgtttgacgg catgttttga gatttctgtt gaccatctct gattgcgggc
    13261 aggtccacgt aaaccaatcg gtagtatttc tgcgtgatcc gtcgacgcac gacgaactgt
    13321 tccgaacatc ttttcagtta gaacgaccca ttcaggcaag gtgaaccctt ctatttcatg
    13381 tttatcaaaa aggacaatat catgtgcttg aatcattttt tatgcatccc attcttcgtc
    13441 cattttggca cggactttat ttgtttcgac acgtccgaat gttttggctg tttcgtttgt
    13501 gtatcgattt gtcagcatcg tgtcatttgt attgcggaca tcttgaatgg cttcattgat
    13561 tgcttcgatg actttgctga cattttgttc tgaagcagct tggtcggtga tatcttctag
    13621 gaaatgatag atcgctccta gttttgtata attattgata tcgtaagcga tagctggaac
    13681 tttttcagca gctgcttcga tttcagcaac cgaacgtttt gtcacacgtg cagcactttc
    13741 tttagacatc gcttggacgg taatttcttt attgtttaaa gcaatcaagc ggcttgattg
    13801 taatccatgt gctaaaaatg cacctgaaat agcatttcct gcaatcaatc caataactgg
    13861 atgtccagct tgacggagtt ttgcataagc agcagcacta ttagccaaag ccacatggat
    13921 accgatcaat tcttcgttat agccgtatgc ttggcttggt acatcgatca caacgatgat
    13981 tggccgtttc acttttttgt ctttatcttc ttcgtaaacg tgatccaaaa tttgtcccat
    14041 gcggaaacct tcaactaaac caacttcacc gtttcgtaca cggtacatcg ggtttttagg
    14101 atcaggtacg attgatgtca cgatggcttc ttctatgaat ttacttgatt taccataata
    14161 cacagtacta atagaagaag ttggattttt catgccagtt agtgccgcaa accaatcgta
    14221 accaacactt gctgcagctg gttcatttcc ttctgctact tctggtaagt caattgattt
    14281 tgcttgatgg ttagcaattg cttcattgta atcgtcaatg gccattggtt ttgaaagatc
    14341 taaagaatca agtaatgata aataaaatgc tgagctttca ctgcgatgcg tgtcttttcg
    14401 ttcgtccatt gctgcgatga cagcagcttt gattgcttct acactatcag tcactaattc
    14461 atcaatgatt tttgattcta cccgttgacg agatcctaat gtattccaaa tcaagtcttt
    14521 gtcactagaa tcaaattctc gaacgcctgc ttcttgttca ataacttctg gtccattcaa
    14581 accgactctc gctttttttg ttgcgattaa ataactcatt aatgctgaag taatcgacat
    14641 accgccaaat gatcctacac gtcctggaat caaaccgatg actggtacat attgttttag
    14701 agcaatgatt acatttccga tttctgaaat agataacagt ccgtagtttg cttcttgcaa
    14761 acgaacacca cctgtatcta gtacaatgac tggaaagatc tcatttcctt ttttattttc
    14821 ttctaacaca tgttcaagtg cagcaacgat ttttgcaccg gaaacttcac cgataccgcc
    14881 accttggaat tttccttcca tagcgattac tactgcttgt ttgccattga tcgtgccacg
    14941 gctgacaacg atcccgtcat cactttcagg gacgattcct tgtgctggta aacttggcgc
    15001 gatgatttga tcgaatggat caatcaattc acgtccattg tcaagtagag cgttcactcg
    15061 ttctctggcg tttaattcta caaaactatt tttcatctga aagttcctcc attgcttgcg
    15121 tcaatcgtag gtacacaact cctggtgtag caccgaagtc attgatcacg atatctgctg
    15181 aaattggata acgatcaaaa aatctcgtta atacattgtt ccatacttcg ccgaagccat
    15241 cgcttcctgt acaaattttt acagtagatt ctgttttttc tgttggaaac atcaagattt
    15301 ccaaatctcc tgaaccgacc actcctacat ggattttctt attgatcggc tgtttcgttg
    15361 gatatttata agctaattgt tccatggatc tctccccttt ttgtaaaaca gagcatacac
    15421 gttctgctta ttcgtctttc ttcttccatc tgggtaaatg gcgacaaaag gtttttctcg
    15481 ccaattttat gaccaagttc tgaatttcat tggtggttca tacaggccat ctgaccattc
    15541 gatcaaatca tcgatggttt gtgcagctaa taatgaacgt ttcgcttcac tacgtttgat
    15601 gcctagatct tctggtaatt tcacgatgcc ggctgcacgc aagctatcta gtgattcttt
    15661 cgtgctagtc attccaagag gtgtaacacc gccaattgct gcaattgctg cttgtcgttc
    15721 ttcttttgtt tttgctttgt acaaataagc aattccttct tctgtcacga tatgtgtagt
    15781 atcttcgcta taaatcatca ctggtgcatt ggctaagcct gcttgttttt gaacagctag
    15841 tgcatctaat tcttcgacaa agactggttt tttatttgaa ccatatgttt caaccatttg
    15901 aacaactaat ttttggcctt tccctaatgg atcatcattg tcgcgaagtg attgccatgc
    15961 tggtgtagaa tgacgacgac cgccaggatt atgtcccatg ttaggcgcac cgccaaaacc
    16021 agataaacgt ccttttgtta cggtagaaga gtttccttcg taatctaatt gcaaggttga
    16081 accgataaac atatcgatgg catattgtcc tgcagcttgt gataatgttc ggttactgcg
    16141 catcgtgccg tctttaccga caaaaaagac atctgggcga gcagcaatat atttttccat
    16201 accaacttcg ccaccaaaac tatggatgct ttcaacccaa cctgattcga tggctggaat
    16261 catcgttgga tgtgggttca atacccagtt cttcgcgatt ttccctttta accctagtga
    16321 ttcaccataa gttggcagta acaattcgat agctgctgta ttgaatccaa taccgtgatt
    16381 caaagattgt acgccgtgtt ctgcgtagat tcctttgatt gccatcattg ccattagaat
    16441 ttgcaattct gtgatgtttt gaggatctcg tgtgaataaa gcttctaatt gataaggttt
    16501 atctgctttc acgacaacgt ctacccagtc accagggata tctacacgtg gtaaagaatc
    16561 gacaacttcg ttgacttgaa cgatcacgat accatctttg aaagctgcag cttcgatgat
    16621 tgttggtgtt tcttccgtat tgaaacctgt gtacaagttg ccgtctttat ctgctttatc
    16681 tgcagctact aaaacaacat ttggaatcaa atcgatgaat aagcgtccat aaagttccaa
    16741 ataagtatgg atatcaccta atttcattgt gccgtcttca atcatttggg cgacacgcaa
    16801 gctttgtgca ccagcataag agaaatcgat cttgcttgcg attccttttt caaaaatatt
    16861 caaatgttct ggtcgtgaga tactagacat gatcaaatgc agatcattca ctttttctgc
    16921 atctactttt tcaagacttt ctgaaagaaa acttgcttgt ttttgattgt cgccttctaa
    16981 tacaactttg tcgccgggag caatcagttt ttccattact tctacgattt tttcagtttc
    17041 tacatacttg ccattgagca agttgctgat tttttccaat cgctgcgctt ttttttcgcg
    17101 tttagaagcc cagttgcggt ttggtttttc cataattagt catctccatt tttgatatag
    17161 aaacgtggcg tcatcaatac catgttggtc tagattgatc gtacggatcc ctttatctac
    17221 accaaatttt gtatgaatca gctttgtcaa agtattgccg gggggaaatt caatcgtcag
    17281 atccattcct aattcttttg ccaagtccat catttggttc cactgcacag ggaaagctag
    17341 gttttccaac aaatctttac gaatctcttc tgcgtcctga gtggctcttc catccgtatt
    17401 ttttatatac aaacatctag catcagatag atggacagac tgcatatacg gcttgaattt
    17461 cttgaccgta tcattcatca agagacagtg agatggcaca gggactttca gcagcttcgc
    17521 tgtttgcgct tggtgccgtt tggcttctgc caacgtaacg gataaacctt cgatactccc
    17581 agataacgta tgttgcatcg gacaattttc atttgacaga taaactggat ggtcaggatc
    17641 aaatgttttt gctacaattt cctcagcttc gcttctcgtc acacctacta ctacgcccat
    17701 tgcataaccg cttggataag cttctttcat taagactgcg cggtgataga ctagttccgc
    17761 tgcattctca aatctcaaag atcgacaagc aacagctgca caaaaagcac caatcgaatg
    17821 tcctgctgca atatcaggct gaataccgtt tttttccatt tcatctaaat aaaatactgc
    17881 ttttgtcagt aatgctagct ggataaaaac cgtatcttga tagttttctt ctttatcaac
    17941 taaccgatac cccgtcacat tttctaaacg ttccatgtac atttcaggga tatccgctaa
    18001 catccctaac ttctgactgc cttgtccagg aaataagaaa cctgtactca tgagcctcgc
    18061 ctcctcagca cttacttttt ccactgctat cttagtcaat ttctgctata ataaaaaatg
    18121 ccaaaaaaag aaaaaaccat aacaaaaagg tatggatggg atggataatt atgaaattgc
    18181 ttcatctgca gtattttaaa aaagtattcg aaacaaaaaa tgttacccaa gcagccaaag
    18241 aattatttat ttctcaacct gccttgagcc gtgcgatcaa acatttggaa aacgaattag
    18301 gtgtcccttt gttttaccat agcggcagaa acattgagac gaccgtttat gcagaagaat
    18361 tttatccata tgcagtcaag acattagaca cattagaaga aggaatcaaa ttattgtctt
    18421 cggtcaatga gaagatcgtt acttccgtca tattgtattt agaggtagcc tctgtttcta
    18481 ttccgaactt ggtccgaatt tttatggaaa agcatcctga tatccaatta acgatcatgc
    18541 aacatcattt accagatgat attaccgatc ctgtactgta catcacttcg gagatgaagc
    18601 ctggcatgac caatattcct atcatgaaag aacctgttta tgtcgccatt cctaagaaac
    18661 atcctcttgc taaaaaagaa catctgcaat tagaagatat ccaacgcacc cccatcttga
    18721 tgctgtcaaa gagcaacgcc ttccgtcata cactggatgc agcacttgaa gcgaaaagta
    18781 tcgaattgac gatcagttcg acgacagatg atcctgcgac attacgttct attgtcaagc
    18841 aaggattagc tgttagcttt ttcccaaaag tttcttggtc ttacgataaa aatgatcctt
    18901 tcgtcttgcg ggaaatcaaa gatttgccct tgacacgaac aatttattta tcttctagct
    18961 tcacagaaga tcatcctttg atcaaaatga ttgcaaaaac gatgcgggaa ttttatttag
    19021 gaaaataaaa aaaaaagagc tgggacaaaa ttcttgtcgc agctcttttt tcgaacaaac
    19081 ggtgttccag aagtaatttc ttagaaaata agccgaaatt tcccaaaaat ttgagaagca
    19141 attttcgtaa attccttctt atttcttgaa gctaaacatt tctgtcacaa cctcttctct
    19201 attgtcctag acattcattg cgttgattta aaacttccat caatcaaatc tgctagttca
    19261 tctagctttt ctattttacg tagatcaata gagatccaat gttctttatt catgtgatag
    19321 gctggaaaat acccctcttt tcctcgcaaa attccgccaa gttcaggatc gaccttcaaa
    19381 tcaagaatat tgatgatcct atggtcactt gcatcatcat agattttttc ttctggaaca
    19441 gacatcagga ccccaaacca ttttttgctt ttttgatgtc tgaatacgca ataatcagga
    19501 aattttttaa aaatatattc tggagacaaa ttgtatgttt cattgatgtg atcgattagt
    19561 tcttttttcg ttagcatctg cttccatctc tttcttattg attcgcttta ttcaaagata
    19621 ccacgtaaga atagaaaaat gaatacaagt agttgctaat caaatacttc gatatttttc
    19681 ttttttatca actcttaaat gttcaatccg cctgtgttgg tcttgctacc atctactatt
    19741 aaaatgttta ttattgtttg cccacctatt ttttgatttt agtggtatca tttttcaagt
    19801 tcatgatgaa attcttatca tcagtaaatg ctccagaaaa ataaacaatg attgaaaaat
    19861 cagttgaaac ttgaccaccg tttattttct ccgtattctt agaatagaag ggagtctgat
    19921 tgatgtattc actaatgaaa aaaatcatta ccgaaaaaga tattcgacgt catgtttctt
    19981 tagtagaaca attgctgaat catacaaaga taaccgtaaa tgaattagct gaaatcatag
    20041 ggacaaccga acgaacgatt ttttctgacc ttcaatctat tcgctctcag ctgcccgaag
    20101 gctgggacat catcagcgat caagcaggaa tcagtttgca aaaccagcaa aatttattaa
    20161 caaatgacct ttgggagatt tttttcaaac aatcagtcag tgtcgaactg ctgaaaaatc
    20221 tgctttttac aaaaaaagtt gctgtccctg attttctagc cgaccatggg ctttcttatg
    20281 ggacattgaa aagacatgtg actaaaatca atcagcgttt agcaagttac gacttgcaga
    20341 tcgatttgac gaaatataca gcttgtattc ttggaaaaga gcgtgcgatt cgtacttttt
    20401 atcatcgatt gctgattcct ttcactcata acaactattt tttcgaggat tattcgatcc
    20461 atgaatccca ttattttcaa tttttaagaa acctcagtca aactgaacta gcggttgaaa
    20521 cagaagagat tttcggaaca tgttggttct ttatcaatac gatccgtata aaagcgaatt
    20581 gccgattaga ttcttctatc cataccaaca gcacactctc ctccctgtat gactctgcat
    20641 tgaaaaaatt gtatttaacg gaaggtattt atcttaaaga cactgagctt tcttttgctt
    20701 ctttctgctt tctggaaagt tggaattaca acaataacta tggacaagaa accgctcgct
    20761 gcctgcatca ttctccattt ttagaggtcc tcgaaacgtt tgtagaggaa ctagcttcag
    20821 agctgtctct tgatcaacta aaaaaaacat ctctgactga taacttggct ctcctcatat
    20881 taaaatatca tgaatcgccg atattgattg aagcattgga ccgtcaatac catcgttttt
    20941 tagaaacata tgatcagcaa tatgcacact tgtatcaaca gaaagagaat ttactgaacg
    21001 atctaaaaaa agaaatagaa atcaatgaac caaattactt tctccaactc ttgtctctac
    21061 tgatccaaca aacgattttt tctatcaaac cgaatttgtt caatgtctat tttattttcc
    21121 aaggagaacc ttcatggaag gcgttcttgc agcaggaatt aaaagattac ttaggaaaac
    21181 gtgtctgctt cttacctatc gaactaaccg atctttccac tatttctttt gaaaaaacag
    21241 atatcttgat ttccaatttc cctctagatt ctctagatat ccctattgta tacatttctt
    21301 ctattccaac gaaaaatgag ctcaatcgtt tgactgaact cactttcaaa acttttttat
    21361 agtaacggct agcgactgta ttctctagct gttttttctt ttttgcgaaa cagtttgttc
    21421 atgatccctt tttctatttc tacaactaag aatagaagaa tcacatttgc tccaatcaat
    21481 aactgttgtg tcatcgttaa acttactgta ccgatcagct gctggccgac tggtagaaaa
    21541 ataaccatcc cttgcaataa agcaaggata cctaatgaaa gaaaaagcgc tttgttttgc
    21601 aaaagacctt ggttcaatga cggcttggac aactctctgc aattgatcat gtaaacagct
    21661 tgagctaaga cgatattttg cagaagaatg gtttgctgta agctttgccc ttcaaactgc
    21721 atagccaata aataggcagg aaccatgatc agtagggaga catagatgat ccgaaacaaa
    21781 tgataaccag ataggattcc ttgctttgga tctcttggtg ggcgcttcat gatttctgga
    21841 tctggcgact cgaaccctag tgcataagat aaagtgattg tcgttaccat attgacccat
    21901 aaaatctgta ctggggacaa aggtagcggg cgattcagca taagtgccca aatcaagatc
    21961 aatccttgag ctaatgcagt tggcaagaag aaattgatgg ttttttgcaa attatccaaa
    22021 atccgccggc cctcttttac ggcttttgca atcgtatgga aattatcatc tcctaaaacc
    22081 atatcagctg cctgtttgct tacttcacta cctttgattc ccatagcaat tcctacatcg
    22141 gatcttttca aagcaggtgc atcatttacg ccgtctccgg tcattccgac gatttctcca
    22201 tttttctgca aagctttaac aattcgcagt ttatgttctg gtgtcgttcg agcaaagaca
    22261 tcgactattg gcacttgctg aatcaactct tcatcagaca taagatccaa atcgatccct
    22321 tctaaaactt tggcagtatg ttttaagcca atttgttccg caatcgcttt tgctgtgtct
    22381 ttgtgatccc ctgtaatcat tttcactgag atccctgctt gttggcattc ctttacagct
    22441 ttgactgcac tttcttttgg cggatcgatc aatccggcga tccctgcaaa agttaattgg
    22501 gttaaagtct catgcgtgag ttcctgtttc gaatctactt ttttataagc aaatcctaaa
    22561 acacgctgac cttttctcgc aaattctgca gctgtctgac tccatgcttc ttgttctgca
    22621 gaagaaagag ttgatttagc aaataatact tctggtgcac ctttgacgta aatcaccgct
    22681 cctttttcct ccgcatgcat cgttgccata tatttgtatt cagaactgaa cggaatcttt
    22741 gcttctattt ttttcaaagg tagattggct gtttctgtgt gtcgaatcag cgcaacttct
    22801 gtcggattgc cacttaaatt ttctgttttc tgttcttctt ttgtttttaa ttcctgacag
    22861 tttttcataa tggacaacac gcaggcttct tcttttgcag ctacatccac aactgtcatt
    22921 tcattttttg tcaatgttcc tgtcttatct gaacagatca ctgtcataga acctaatgtt
    22981 tctaccgaag gaagtccttt gatgatcgcc ttttcttttg ccatttcatg tactccaacg
    23041 gatagaatca tggtcaaaac agctggcaat ccttcgggta ccattgacac caccaaagca
    23101 atcgtagccg aaagaaggat atgccaatcc ataccatatc gaaatgtcgt aaaaataact
    23161 aagaataggc tcaaacaaag aatgccttga aaaatttgtt tgtttaatcg gttgattttt
    23221 ttaatcagag gcgtcgtttg ttgttcgacc gattgcaatg cttgattgat tttaccgatt
    23281 tctgtcgtgt ttcctgtttc tacaacgaca ccgactgcag aaccagattg tacaagcgtt
    23341 cctgaaaaac ccatattttt ctggtcgccg atcgattctt cttctgtcaa aacggcatga
    23401 cttttttcag atgcttctga ttctcccgtc aaaatcgatt cttccactaa taaatcatgt
    23461 gcttcgatta atcggatatc tgctggaacg acatctccgg cacgtaagac aatgacatct
    23521 ccttttacca gttctttcgc agggattttt tcttgcatcc cgtttgatag tactatcgct
    23581 tcttgtccca tcagctgctt caaaccattc aatgattctt ccgcttttct ttcttgccag
    23641 taactaacga gtccattgac gatcactacg aataaaataa tacttccttc aatatattca
    23701 ctagaagcaa atttcaaaaa agaggcaatg atcaacacga tcatcagcag atcagtaaaa
    23761 tgctttgcta tttttttcca tcgttttgtc tgctgccttg cttttatttc attatatcct
    23821 tgatcagtca ggcgtttctg tcgttcttct tgcgacaagc cttctgttga tgtttgatag
    23881 tttttcaata catcttctac cgtcaatctg taccagttca tcctatcccc tcctgtttaa
    23941 ttgatactgt tattatacgc agggaagttt tttcaggcgt atcgattatt tcaggggggt
    24001 ctgaaataat tcgttttgca aaatgtatct gggcctaact gatatttcta ttgcttattc
    24061 ttttatatac aaaatttaag aatacaaacc agataaccaa taaaaaagga ataaagcgtg
    24121 tcgtgctttc gaataacaag ctgaaagcaa ttgaagtaaa aaacaccaac aaagcaagat
    24181 ctttatatct ttgtgtagtg ttccgtttct tttctttaat aaattgaaca tgtgcacaca
    24241 agatcgaaga ccacacaaat agaaaacaac tcgttgtcat gcctgccaaa agctgaaaaa
    24301 acaatcgact atcagctaca aaaaaatgta gacaaagacc ggcaaaaaat aacattcctg
    24361 taattaaaat accgtgtaaa ggtacgcctt tcttagaaag cgcacaaaac caatggggca
    24421 aacttttttc ttttgctgtt tgatataaaa tcctgctagt actgtagact gcactatttg
    24481 ctgaggacaa agaagcaacc aaaatcatta gattgacaat tgctgctccg ccttggcaat
    24541 ggatcacttg cagcatagtc actaaaggac tttcttttaa atctaaacaa tgccatggaa
    24601 caacaaataa aatcgacaaa atcgttccta tatagaaaaa gagaatacgc caaggcagac
    24661 agttgattgc ttttttcaaa ttcttttgcg gatcacttgt ctcgccaacg gttaagccga
    24721 tcatttcaat tccagcaaaa ctgaataaaa ccataggaaa tgagactacc attccagcaa
    24781 agccaaacgg tagtaattca tcgatctgtc caaaattttc tttgattccg atagactggg
    24841 tatgaaaatc tgagacactc aatcctatac ccaataagat aaaaaggaaa atccccaaga
    24901 tcttgatgat tgagaaaaaa gattccaatt caccaaacca cttgactgca acaaggttga
    24961 ctgccattaa gatcattagt gcgctacctc cgcttatcaa agctggtaca tgtacaaacc
    25021 agaaatggat atacaatcct attgctgtta attccgcaag tccagctcca agccagcaaa
    25081 gccaataagt ccaattgaca aaaaatgccc actttttgcc taaataatag tcaacgaaat
    25141 cactgataga gttaaatgta ctatttgcta aaaataaatc acccatggcc cgcatcaaac
    25201 cataaatagc caaaccgcat atcaaataaa cgagaataac agctggccct gctgatgcaa
    25261 ttgcctttgc tgaacctaaa aataatcctg ttccaattgc gccacctaat gcgattaact
    25321 ggatatttcg attagttaga gtccgtttca ttgccggttt ttccatcaag ttcacctttt
    25381 tctttttcaa gcagcatggc gacttcctgt tcccaagaag gtttctttat tttttcaata
    25441 cagagtggta ctacaaacaa aatgacactg atggctgtga ctgcaattag attgatccat
    25501 gttccagctt tcatcgtcag tccgacagaa aatccgattg ccgcaaataa aacaataacg
    25561 acagtccaaa ctccaagatt gctctttttc tctttttttc caattctaaa tggtcgattc
    25621 aactttgcat gtgtataacg caatctcaaa actgcgatcc ccatgatgac atagacgata
    25681 cagtagataa cagtagttga atttacgagc atcaaaaatg cttgattgac gccaggaatc
    25741 agcaaataaa aaagtgcaaa gattgagata atgacagact gagtcaaaat aacggatttg
    25801 gatacgtcaa attgattcgt cttccaaaaa ttgaattttg gtggatacgc accttttcta
    25861 gcactttctg tcactgtctt tgctggtcct gaggcccaag cagataattg gactgctaca
    25921 ccaatgaaga caagtaaact aaacagattc acaataagat gtggcaaacc aagaatttgg
    25981 caataaatcg aaatagattg cgcgatatta ttcaattcca tttgtccttt tggtacgaca
    26041 tttgctacaa ctaaagcatt caaaatattg acgaaaaata taaaaattaa cgcggcaaat
    26101 actccgcgaa tatacgtttt aactggattt tccaatcgag tgatatacac agaggacatt
    26161 tcgataccag taaaaataaa catgatcggc gcaaaataaa caaatgagga tgcagtcgta
    26221 gtatctggta ttaatttgtc ccatgaaaaa ctgcctaatg ttccggttgg tacgatccca
    26281 actttgatcc acgcagcaaa ccctaataag agcatcatcg tcaaaggcac gtacaatcct
    26341 aaccaaacac cgacttttcc gccgattttt gccatatcaa atttcaaatt caaaaaagtg
    26401 atgatccaat aaacgactaa aatgacaact aatgtaaatt tgctgttcaa accaagcggc
    26461 acattatcga taatattacc aaacaacgga gcgagtgctg aagcgaccat caccatccca
    26521 ggaaacattt ggacccataa cagccatgag acaacgaacc cccatttttt tcctagagca
    26581 ttcgataccc aaagttctgg accacctttt tggggaaaca ttccagcaaa ttcaccagag
    26641 atcaatgaga ttggaaaagc gtacaataat actgcaacca gcatgtaaaa aatcatcgtc
    26701 cagctcgttg ctgctacatc tggaatattt cgtacacttg ctacaagggc gcaggtcatt
    26761 ccgataaagg tcatggtcgt gatttgcttt gatgaaccag tatctttcat ctttattcct
    26821 tctttcaata gcgacatatg tttttgtttt cttttatttt tgttcaagaa cagaaatgac
    26881 aaggtttcat cgattcagta caaaacccag tatcaccgaa acatccattg aacgatctta
    26941 gtgaactggg tttgatacat gaaacgaacg atccaactta tagaacgttt tataaaaaag
    27001 aatatcttgg cgaggaatat atgaaaaaca gggggaaata caattgtttt gatgaagcaa
    27061 ataattgtct gttctccata tgtctcttgt tattctttca cacaaaaatc agttctattg
    27121 gatgatttcg tttagtttct tttccattgc tttggcgatt ttttttgtat aaaaattgaa
    27181 aattcgatca tcattcaaat aaggagtcat gatagccgca cgtagcagag tgattttctg
    27241 ttcttttttc cattcttctt tcgttagacc cattctttca acaaaacgaa tcggagaatc
    27301 accgtaatct tcttgtgtaa atgtagtatg agaagtaata aaccgttcac cataaacatc
    27361 gccatccata taagaagacc gatcgaacat tttttcattt aattcattga tagcgttcaa
    27421 atctgtacag ccttgttctt tgaataccca attgaccata ttaaaatcag gatgattcaa
    27481 tggatagact tcgattgttt ttcctttaac agtaaaagtc agatgatcga gaaattctct
    27541 gaatctttga gctgcctcga ttgaagcccc aatcaattga ccatatccgg tcacattcaa
    27601 tggcaataca cgatgtgctg tccagactgc ggcggcggtt gctccagctt ttgatccttc
    27661 caaaatataa gctcctaaca tatctggagc ctttactgat ttttcaaaca catatggcgc
    27721 gaaataagaa atgatatttc tcatattttt atgtttgatt acgattcctc cagcagcata
    27781 agggacatag cccatcttat gaggatctat cgtgacagaa tccgcttctg aaatagctct
    27841 gaacccttca tatacctctt tgtcaatcgt tacgcagtgg tggaaaacat gatgttcttc
    27901 aaagaattca gctaaggaag catacggcac aaattctcct gcttcgttta gaaacagtga
    27961 acgcgcatag ccgccatatg cggcatcaac atgtaaatag aaatagatcc cttcatcttt
    28021 taacctttct cttaattgga cgattttatc tacactgtct acttgacctt cttctgtcgt
    28081 tcctacgaca gcaaccacac ctagaattgg gattttttgt ccagctaatt cacgaattgt
    28141 tttttccaaa gcattaatat ccattcgata atcctcttga actgggatag caaccatctg
    28201 atctaagccg actccgcaaa tatctaacgc cttcatccaa gaatagtgtt ttgtttgagg
    28261 gaccagccat ttccctaatc tttggatatt ttttccgcta cgggaagaag ctgctttgac
    28321 ctcatccatt tcttcgtccg taaagctttc ggtcatttcc aaaatttctt caacagataa
    28381 attcaaaagt tcccactcag acattttttt cactttttca ggcagcactt ccttgacagc
    28441 taaaggaatc gatttgatgc aacgggcgta ccataatcct tcaagattag caatagaacc
    28501 atctgctgca atgtgtcccc atccgtctgt catattgaat aatgaagcaa aatcctgtcc
    28561 tacttctgct tccatttgcg aagttgccat agaagattcc aatgcaacat tatttgggtt
    28621 ccacagcatc gcatagttgt aagctaataa agcaggcatc aatgtttcag cattcatttg
    28681 tccccagtaa cggcctgctg aatgccatgg gatcgatcct gcgcgtaatc gctgactgac
    28741 ttcatccaag acctctatca tatggtcgcg ggtggctaga taatctggag tgagcttatc
    28801 tccttcgcta attgcaggca tatctgaagg aatataattt tctctccatc caagatgttc
    28861 atctaccatc ttgttcaaca acattttata aactggtcca ttttcagctt tgtcgcctat
    28921 aaagacggcc ttgatatcca tatctttcat ttttcttccc tccatatttt gttggtcaat
    28981 gtcttgcaga tacatgtgtt tatctgtaac acctcttcac tatagcgcca aaaatgagaa
    29041 tttttttttt ataacatcaa atccacgtag atttttacaa acaaaaataa ataaagtcgg
    29101 atttttctga taattcaacg caaaaaagcg aacaattttt caataaatca gtcttctaaa
    29161 atagataacc taaaaaaagc gaacaaacaa ataaaatgac tgtccttcat tcactaaaca
    29221 tatttttatt tttttatatt gttaaaaatt cgcaatttat tttttaatag aattatctgt
    29281 tttccaaatt ttataacata aaaaaccttt tttaacggca tttatatgct ttttcagaaa
    29341 ttaactgatg atttcaaaaa gaaaacatgt cataatattt cccatattgt gcaaaaaaat
    29401 acaaggcgga ggctctacta tgattgaaga atatattgag aaaaatattt taagacagct
    29461 gtttttatgt gggcaattct atgtaaataa agaagtcaat ctagaaaagc tctccaatct
    29521 cctgcatgtt tgcaaaacca ctttactcaa tgatatcaat aacataaaaa aagaatttga
    29581 agaacagatc gcttatacac atcgggaaaa ggaccgttac actttatatt tttcagaaca
    29641 tatacctcga tgcaagatca tgcagcaatt gtctcaaaat tcgttgtttt taaaaacttg
    29701 tctgctttat ttggaagaag atgaacctga ttatcttcaa ttaactgaat gtgaattcat
    29761 ctctgtcagc aaagcttatt cattaaaaaa acaagtatta gcttatttta atgactgcgg
    29821 gatcgaaatc gaccgctact cccctcgatt cactgaaatg gaaagacgtc tgctgcttct
    29881 taatgtgtcg tatcgtttag gcggttttaa ttcttgggag ctacctgaat cgttttttga
    29941 acgtgccgat cgtttcattg aatccgtaac ggaaaatagt ggcagatttt atgataaaga
    30001 aaataaagaa atcttatcga ttggttttgc tatcagtttc ctacgccagc aagtgtgtgc
    30061 tgtgacaata gattcaaaat ttatcgaaga aatcaaaaaa cgtcctattt acaattatgt
    30121 cgaatcagca tgggaaaaca ctgatttcca aacctattat aaaaaagaag aattcgcctt
    30181 tattttgaca ctattcaatc tttgtaatta tggtttccat tcttatcagt tgattgcgga
    30241 agactttcaa cagctacacc aagtgtttat tgacaatact cccgaaataa aagaactagt
    30301 tgctaccttt gaatctcact tcaatcaaga attattcgga aaccaaccgt tcgaacgtgc
    30361 gctgattcat ctgatgcgtt cggcttggga taactatcaa ttattcatgc cagaaaaatt
    30421 ttatctttta aatgaggaac agacaaattt atataaagaa gtccagacaa ttttttccag
    30481 ctggtccagc cagttacctt atgatctacg gttgaatcct aattgcatgc gggcttttgt
    30541 gatcgaattg agcggaattc tccgtctgac aaaagaacat ctgactatct atatcgtcac
    30601 aaattcggat gttcattatc tgatttatcg ggaagccttg gaagctgtaa ctactttcga
    30661 ttttcaagtt gcaccaacaa tttattcaag tatatctgat ataaagaagt atgcgcagca
    30721 gtcctcaaac cgggtattat gtgaacgtac attatataca ccagatgcgg tccaatatga
    30781 aaatattatt cctatttcga tcaatacgat cgatcgagct atcatttcag ctgtgcaaaa
    30841 caaataaggg gctgtgacaa aaatcatgtc acagcctctt atttgttgaa gatgcccatt
    30901 tctgtaacaa tctcttgata cacatcaggc tcacaacctc acttattttg gacatctacg
    30961 ggtccgatct ctttatttaa aaaataagaa atgatggttc ggataactac caacgccgct
    31021 agttggaaaa tatcctgcaa ggtaggtttg ataatcgatt cgatgatccc agctgaaaca
    31081 agaatttcca aacttagaag tatatagcct cccaacatct ttttaatagc attgttctcg
    31141 ctgactcggt cgctttgcac ttttgtatgg acaaataaat atttcaaaaa atccttgatt
    31201 gatagacaaa caccccaaac caataaacag atagcaaaga tatcaagtcc cagtacaagt
    31261 ttttcaaaat atggaagaag aaactgcatc aactctgtcg tcatcctacc tctcctcttc
    31321 tttgtttttc tttgtcttct atttcctgat tcaagaaata agaaatgact gttcgaatga
    31381 taataataag tgcaagcttc aaaatatctg tccaagtcgg cttgataact gactcgatga
    31441 tatcagcaac gactagtact tccaagctta acaaaatata acttgctagc agttttcttg
    31501 tatctgcatt acgagtatta cgttctgcat agtttttctt gacatgagac caatcaaaaa
    31561 gtttgaatag aacaagaacg atcccgacaa ttaagatacc gatagaacaa atatttaaaa
    31621 tagcaatgat ccattctaga aattcaatta aatttgagcg aaacatatac gctacctact
    31681 ttcttttaat ttgttcctat tcttttattg taaaagaaag ttaccttgtt cgtctattta
    31741 aaagttgttc taatgataac ggttcgctta aatttttctt tttcactctc ccctttttct
    31801 ccctccatcc ataaatcgtg gaagtatcca aaacaacgta gcaagttaaa ataaggctta
    31861 gtccgtcatc agcttttcaa agaagcactg tctcggtgct ttttttgtac ccaaatttct
    31921 aaaaatagaa ccatacacct cctcaaatct tttcttctta cctaccctat ttttatttac
    31981 aggaaaatca gacacttctt cttttttgtc aaaatttcct ttttcttctc gtcctatcat
    32041 ttggcattct aataaaaacg aactatcctt ggatatagaa agagaaaaca aggaggcgtt
    32101 tttcatgagt aaaaaattaa tcaaagtcgg catcggttta ggtttgcttg cccttggcgc
    32161 tgcatatcta ggaaaaaaga caggactatt cgaagatgac agtcatttat atgatgaatt
    32221 cgaatcaatc tagcatccta tttagttgaa tcactacgga catacttgga agacattgga
    32281 ggaatcacaa tgagaaaaag taaagcaata atgattggtg ccggtattgc caatatggca
    32341 gcggctgttt atttgattca agaaggcaaa tggaaagggg atcagatcac cttttattca
    32401 ttggatgatc acggttcaaa tgatggtgca ccgaccgaca ctgtagtgga tgaatattgg
    32461 aataaaaacc atccattaga aaaccaaaaa ggctatattg ctcgaggcgg tcgcatgctt
    32521 aattaccgta cctatgtaga tttgatggat ctgttaagtc gaataccttc tgttactgag
    32581 ccagggatga ctgctgaaga agatacgaga gattttgatg caaaacaccg aacctttgac
    32641 aaagcacgtt tgatggaagg tggcaaagga atcattgatg gcggaaaact gggattcaat
    32701 aataaagacc gtattcttct gactaaactg attgctatgc ctgattcaga agaagaaaaa
    32761 ctagacaata tcactatcga agaatatttc aaagatgatc cacacttttt tgaaacaaac
    32821 ttctggttta tgtgggaaac aacatttgct ttcagaacac gtagttctgc tcaagaattg
    32881 cgtcgctata tgcatcaaat gatttatgaa ttcactcaaa tcgaacatct agtgggcgtc
    32941 aatcgaacac gttacaatca atacgaaagt attatgcttc ctttgatcga gtacttgaaa
    33001 gaacaaggat gcaaaatcat tttgaaccgc cgtgtcactg actgggaatt caaagaaaca
    33061 ccgatgcaag atgaaatcac tgtgactggt ttgaaaatga ccaatacact gactcaagaa
    33121 gaagaacacg ttgtggtcga tgaagataca gcagttattt tcactaatgg gtccattaca
    33181 gattcagcta ctttaggaga ttacgaaaca ccagctgtag aaaatatgga ttatggtgca
    33241 gcttctagtc tctggaaaaa agccagtgaa cgtttctaca acttgggaaa tcctgacaaa
    33301 ttctttgctg atcgagatgc aagcgaatgg gtcagcttta ccctgacgac gaaaaaccat
    33361 ttgttgttga atgaaatcac ccgtatcacg actcaagaac caggaaatgc cttgaattca
    33421 tttatttcaa ccacaccgat cacgccgtta ggccaaaaag atgtcaatat gtctatcgtc
    33481 gtgcatcatc aaccgcattt tacgtctcag aaaccaaatg aaacagttat ttggggttat
    33541 ttcctttatc caagacgacg tggcgaattt gtggataaac agtatattaa aatgaatgga
    33601 aaagaaatgc tagaagaact gattggacaa ctttcaaaag tagatcctgg accagtgaat
    33661 attcgtgaaa aagaaacaga aattttcgac agcgtaataa ataatatccc tgtctatatg
    33721 ccttatgctt ccgcgttgtt caataatcgt gccaaaagcg atcgaccaaa agtcattcct
    33781 aaacattcga ccaatctggc ctttacagga gaatttgtcg agcagcctta tcagatgatc
    33841 tttactgaac aaagtgctgt acgttctgga gaaatcgctg ctttccattt tgcgggtatc
    33901 cctatgtcac gtcttgtcaa aacaccgcgt tacgacaaag atattcctac tctagctcgt
    33961 gccgcaaaaa aaatgtttga ataatcaaga ggttgtgaaa aagctgccct gcatcaagta
    34021 ataagaacaa ccaggctaaa acagcttctt atgtttttga tttggttgga cttattaccg
    34081 cagatgcaag cttttgaaca ccgtttacca agaggttgtg aaaaagctgc cctgcatcaa
    34141 gtaataagag cagccaagct aaaacagctt cttatgtttt tgacttggtt ggacttatta
    34201 ccgcagatgc aagcttttga acaccgttta ccaagaggtt atgaaaaagt cgtttagttt
    34261 cgagtatgaa gaaaaatttt gtgaaaatgg ttcctcacgt ttttgcataa atttgactta
    34321 ataccgagaa actgatccct tctgtcataa cctcttttta tttatgtctg tttctttttg
    34381 aaaacaacca ttttttcaac gtttactctc ttgccaatcc aacatcatct aacagataca
    34441 actatttcaa tgattaccat tacactaaaa aatcggaaac tcacctctcc gaacacaaat
    34501 acataagact atagaatgtt tcatttaaga aaaaaagcgt ttcattatca aaaaggtaag
    34561 atctccttta aaatgaaatt atctgtagta ctaataatgc agacaatttt atgaatctga
    34621 aaggagcatc tccaatgaaa gcagtcactt ggcaaggaaa acagaaaatg gaaatcagaa
    34681 acgtagatga tccaaaaata ttagaaccaa cagatgcaat catccgtatc acagcaacag
    34741 ccatttgcgg ctccgatctg catctttacc atcatggtga agcagttttg gaaaaagggt
    34801 atgttgtagg acacgaacca atgggaatcg ttgaagaagt gggatctcaa gttcaaacat
    34861 tgaaaaaagg gcaacgtgtg gttatccctt ttaatattag ttgtggtcat tgtcatttct
    34921 gcctgaatca catggaaagc caatgtgatg aatcaaatcc agatcagtta tatggcgggc
    34981 ttttcggatt tggcaagttg aacggtaatc attggggtgg tcaagcggaa tatctaagag
    35041 ttccttatgc agactctact tcatttgttg taccagataa tgatcttcct gatgaaaaag
    35101 ttttatttct atcagatatt ttgcccacag cttattggag cgttgaaaac gcaggagtaa
    35161 aaaaaggcga tacagttgtg attctaggtt ctggtccagt cggtctattc gctcaaaaat
    35221 tcgctgttat ggctggagca gagcgcgtaa tcgcagtaga tccagtacag caccgtctag
    35281 ataaagcggc taattacaat ggggttgaaa cttatttctt agaagacacg gcacaagctg
    35341 gaacagaatt gtatgaactg gctaaaggtg gcgcagatgt cattatcgat tgtgtaggaa
    35401 tggacggatt agagcctgtc aaagaaaaag cgaaaaattt agtaagctta caaaacggca
    35461 caatctctcc attacagatg gcatcgcaag ctgtcaaaaa atttggaaca gtccaagtaa
    35521 ctggtgttta tatgactcca gcttcttctt acccgctgca agaatttttt atgcgggata
    35581 tcgctgtcaa gcatggacag gcaccaatta tccatctaat gccaaaaatt tacgaaatga
    35641 ttgcagaagg actattcgat cctacacaaa tcattactca ttcgctgcca ctatcagaag
    35701 cagcacaagc atatgagatc tttgacaaaa aagaagataa aaatataaaa gttgttttga
    35761 aaccttgaat tcagtaaaaa atacaggtga ttttctttat gcccaaaatc aaacgaaata
    35821 cccatgagat cgaaatagga aaagaagaag atatcgaaat acgaggacgt cgcttccgct
    35881 tctatttcca aaatcgttac acattgatct cactggctgt cgatttatta accgggattt
    35941 tctatatttt tggaagcctt gcttccttaa ctaaaatccc cgatcgatat ggcacctatt
    36001 tttatttggc tggtgctatt ttcttgacga ttcgtcctat tctcagaatc gtaaggaaca
    36061 tttttattta cgacgaaaaa aaagcaatga agagaacaag caaaaaaaaa gaggcatgag
    36121 atttgaaaaa atctcatgcc tcttttttca ccctatcgta tcactttgat acagacgagc
    36181 ggtcgtccca ctttcttcct tgaccattgt caaaaatgtc cactcgtatc gttcataaaa
    36241 cgtggtatga tctgtaatta agtaaagttt ttcgtaccca agttgatgca tttcttttct
    36301 gatttccgct aatatccgct tggctatccc ctgcttacgc ttttccggtt caacaaacag
    36361 ggcacaaaga tttggtgtca aatcagggcg gttatggaag tcgttttcaa taacgcccgc
    36421 tcctccgacg atggtttctt tttcatctaa gaccacatac cattgaggaa tactttcctt
    36481 attagcgatt ccagtacgaa tactttcctc atacacaaca gcaggaactc cccattttga
    36541 tgaaaaccac ttcgcacttt gttttactaa ttctgggtgt tctcttaatt tgatccagtt
    36601 atatgtaacc aatgtttaaa cctccgacta aaataaaacg tttacaaaaa ttatacaagt
    36661 ctttatgaaa attagcaatg acaacaatag tgatgtaaaa aaaacggaaa acaatcactt
    36721 gttctccgtt ttttgattaa tcaagcatct aaagcttctt ttcttctttc ttggatcgta
    36781 gctaccttga cgaacggtag atagatcaaa atagaaagaa ccaaattgac agcggctagt
    36841 acaccacctg caatactttt tgttgccaat acaccgccaa taattggtgg agttacccat
    36901 ggaggcataa cggtagctgc tggcactaaa cctgtagaag tcgcaaagta agcaacacta
    36961 actaagacca ttggtgtcaa aataaatgga atgaacatga tcgggttcaa cacgataggt
    37021 aacccaaaca tcgttggttc attaatattg aagacacctg gcgcaatcga caagtttgtt
    37081 acaaccatat atggtttgtt tttacgtcct actaaataaa tagctaacag taaacctaaa
    37141 gttgcacctg ttcctcctaa gttgacaaag ctgtcaaaga aaggtttgtt cacgataaac
    37201 ggtgcgctat gtccagctgc gattgcatta acgtttgctt caatggctgg cgcattgatt
    37261 gtttgcatca aaggatcgac catattcgct ccgtgtaaac caaagaacca taagaatggt
    37321 gtgataaatg caagcagtaa ggcagatgga taagagtttg caagtcccat aaatggttct
    37381 tggaccactg tatagaaaga acctacgata tcatcaacac cgaatgcagc aaaaattgca
    37441 gcaactaaag caaacgcaga gatagtgatc attgctggta gtaatgaagc aaatgctttt
    37501 gcaactgccg gtggcacacc atcaggcatt ttgatgatca atttttcatt tccgcttaaa
    37561 cgtacaaaga tttctgttga aataatccca acaataatag caataaacaa accgtttgag
    37621 ctcataccta atgctccgcc taaagcgaag aaagagccta aagaaatcac acctgcggaa
    37681 acaccgtctt ttcggtagcc gttagctaaa tgataagcaa gaaagcaatc aagacagaaa
    37741 gaatggcaaa tgtaccgttc caagcagcag ccccgaatcc tttccatgat tcaccaccaa
    37801 aaattgagtt catcaattct tggaacccgg gaatcggtaa gttattgatc attactgcta
    37861 atgctcctaa gatcattaac ggcatactca ccataaatga gtcccgaatc gcaactaaat
    37921 gccgttgtgc accaattttt gatgctacag gtataaaatg tttctccata aatgcaacaa
    37981 atttatccat aaatcttccc tcttttcttt actgtagatt acgcgttcaa tcgacgatac
    38041 agatcaatga tctcttttgc taaatctgta aaagctatgg atgtcatcag atgatcttga
    38101 ctgtggactg tcaaaagcgt gacttcaacg tgatttcctt gtgcttcttg tgtcagcatc
    38161 tctgtttgag agtgatgtgc ttggactaaa gattcctctg catctttgat tttttcatct
    38221 gctaaagtga aatcaccttt tttcgcagca gcaatcgctt ccattgcatc gctttttgca
    38281 tttccgccaa acatgatcag acccatgatg gcttctagat ttttttgatc ttccatttat
    38341 tgtatcctcc gataattttt attatttgtt tattagtttt tctgcttggt cgagtacttt
    38401 ttcaccattc atcatgccat agtcagccat attgattata tctaaaggaa tgcctttagg
    38461 tgctagcttt tcagcaaatt gattttgcat aaagcgtact tgcggtccta aaagtaaaac
    38521 gtctacattt ttagattcta gttgattatc cgcatctgaa gcagatacag caaagatgtc
    38581 tgcttccatt ccttttgctt ctgctgcctt ttgcattttt gtgactagga gacttgtact
    38641 cattccagct gaacatacaa gcatgattgt tttcttttcc attttaaaca cgttcctttc
    38701 tatacttaca cttgtatata agcaagtagc gtgccaactt tgaaagcgct ttaaaaagtt
    38761 gatatcatca catttaaggt ctataccaag ttgcatacac actaaaaaag aaagtgcaca
    38821 ctaaacacac aaattaattt ttattacctt ttttctttta acgtgcacaa aaaaagttgt
    38881 gacacaaaac ttgtcacaac tt
//
LOCUS       DAZUMM010000018        37452 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000018 DAZUMM010000000
VERSION     DAZUMM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.018"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            160..390
                     /locus_tag="ACG8E6_000862"
     CDS             160..390
                     /locus_tag="ACG8E6_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFS5061303.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            487..1788
                     /gene="murA"
                     /locus_tag="ACG8E6_000863"
     CDS             487..1788
                     /gene="murA"
                     /locus_tag="ACG8E6_000863"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS5061304.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1801..1977
                     /locus_tag="ACG8E6_000864"
     CDS             1801..1977
                     /locus_tag="ACG8E6_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS5061305.1"
                     /translation="MSNQRYIILTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2112..2408
                     /gene="yidD"
                     /locus_tag="ACG8E6_000865"
     CDS             2112..2408
                     /gene="yidD"
                     /locus_tag="ACG8E6_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFS5061306.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2592..3059
                     /locus_tag="ACG8E6_000866"
     CDS             2592..3059
                     /locus_tag="ACG8E6_000866"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5061307.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3144..4028
                     /locus_tag="ACG8E6_000867"
     CDS             3144..4028
                     /locus_tag="ACG8E6_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5061308.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4142..4378
                     /locus_tag="ACG8E6_000868"
     CDS             4142..4378
                     /locus_tag="ACG8E6_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFS5061309.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4466..4546
                     /locus_tag="ACG8E6_000869"
     tRNA            4466..4546
                     /locus_tag="ACG8E6_000869"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4500..4502,aa:Tyr,seq:gta)
     gene            4553..4624
                     /locus_tag="ACG8E6_000870"
     tRNA            4553..4624
                     /locus_tag="ACG8E6_000870"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4585..4587,aa:Gln,seq:ttg)
     gene            4749..4919
                     /locus_tag="ACG8E6_000871"
     CDS             4749..4919
                     /locus_tag="ACG8E6_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061310.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5139..5846
                     /locus_tag="ACG8E6_000872"
     CDS             5139..5846
                     /locus_tag="ACG8E6_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5061311.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5906..6550)
                     /locus_tag="ACG8E6_000873"
     CDS             complement(5906..6550)
                     /locus_tag="ACG8E6_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFS5061312.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6677..6964
                     /locus_tag="ACG8E6_000874"
     CDS             6677..6964
                     /locus_tag="ACG8E6_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061313.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7072..7392
                     /locus_tag="ACG8E6_000875"
     CDS             7072..7392
                     /locus_tag="ACG8E6_000875"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5061314.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7473..8786
                     /locus_tag="ACG8E6_000876"
     CDS             7473..8786
                     /locus_tag="ACG8E6_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS5061315.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8783..9502
                     /locus_tag="ACG8E6_000877"
     CDS             8783..9502
                     /locus_tag="ACG8E6_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFS5061316.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9630..10181
                     /gene="raiA"
                     /locus_tag="ACG8E6_000878"
     CDS             9630..10181
                     /gene="raiA"
                     /locus_tag="ACG8E6_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFS5061317.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10403..12937
                     /gene="secA"
                     /locus_tag="ACG8E6_000879"
     CDS             10403..12937
                     /gene="secA"
                     /locus_tag="ACG8E6_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFS5061318.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDIQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGDE
                     DAVIQSKMLTKQVEAAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIVDFAGSTLVHEDSISLTDIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKDAN"
     gene            13064..14165
                     /gene="prfB"
                     /locus_tag="ACG8E6_000880"
     CDS             join(13064..13135,13137..14165)
                     /gene="prfB"
                     /locus_tag="ACG8E6_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFS5061319.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAVMQEEAEARI
                     NALDEKMNTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14270..14956
                     /gene="ftsE"
                     /locus_tag="ACG8E6_000881"
     CDS             14270..14956
                     /gene="ftsE"
                     /locus_tag="ACG8E6_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFS5061320.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14949..15833
                     /gene="ftsX"
                     /locus_tag="ACG8E6_000882"
     CDS             14949..15833
                     /gene="ftsX"
                     /locus_tag="ACG8E6_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFS5061321.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEKAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFYQLINPSLLRSHYSLIHPENIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            15985..17433
                     /gene="cls"
                     /locus_tag="ACG8E6_000883"
     CDS             15985..17433
                     /gene="cls"
                     /locus_tag="ACG8E6_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS5061322.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDTLGE
                     EVREALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPEFFPTIHSTGDMAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEVYENRGMMIKMKEGLARLISPLL"
     gene            17770..18627
                     /locus_tag="ACG8E6_000884"
     CDS             17770..18627
                     /locus_tag="ACG8E6_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFS5061323.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQAENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18742..19662
                     /gene="pstC"
                     /locus_tag="ACG8E6_000885"
     CDS             18742..19662
                     /gene="pstC"
                     /locus_tag="ACG8E6_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFS5061324.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19662..20546
                     /gene="pstA"
                     /locus_tag="ACG8E6_000886"
     CDS             19662..20546
                     /gene="pstA"
                     /locus_tag="ACG8E6_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFS5061325.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20561..21367
                     /gene="pstB"
                     /locus_tag="ACG8E6_000887"
     CDS             20561..21367
                     /gene="pstB"
                     /locus_tag="ACG8E6_000887"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS5061326.1"
                     /translation="MKKYNWNETNVITMPKDKEVALYTNDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYKDVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21383..22141
                     /gene="pstB"
                     /locus_tag="ACG8E6_000888"
     CDS             21383..22141
                     /gene="pstB"
                     /locus_tag="ACG8E6_000888"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS5061327.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWEDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22154..22831
                     /gene="phoU"
                     /locus_tag="ACG8E6_000889"
     CDS             22154..22831
                     /gene="phoU"
                     /locus_tag="ACG8E6_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFS5061328.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23395..24087
                     /locus_tag="ACG8E6_000890"
     CDS             23395..24087
                     /locus_tag="ACG8E6_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS5061329.1"
                     /translation="MLLIGGKQGVFALGSLIINIVLLLLLLLLFTKTEGISLLNIAIL
                     FVVIGIIVSILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKQQPKISAKQLWLSGRQVGRDVI
                     GSMANILLFVYISGAIPSLLLYLGNQWSFKETVERHLSLELLRVIAGSLGIVLSIPIA
                     VLFFLAVRRLKK"
     gene            24084..24851
                     /locus_tag="ACG8E6_000891"
     CDS             24084..24851
                     /locus_tag="ACG8E6_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS5061330.1"
                     /translation="MNVLIILVGIFAISVLFVGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILVLSIIGFLLIAVVILFFINGYNLKMRAAFASILIFLFCFLLLIPITDFLAI
                     QGFTSIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     MSFNQLRHASFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDISFQQIINEKSFVL
                     EYTMAILTTLAALLVLPLTCVTTAYLFTKKKEHLKME"
     gene            24951..26519
                     /gene="liaX"
                     /locus_tag="ACG8E6_000892"
     CDS             24951..26519
                     /gene="liaX"
                     /locus_tag="ACG8E6_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFS5061331.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDELENGEEKISEPVDPKEKERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEATGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSDDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVNQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATPENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDSIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26695..27003
                     /locus_tag="ACG8E6_000893"
     CDS             26695..27003
                     /locus_tag="ACG8E6_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFS5061332.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF
                     "
     gene            27003..27353
                     /locus_tag="ACG8E6_000894"
     CDS             27003..27353
                     /locus_tag="ACG8E6_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS5061333.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
     gene            27519..28457
                     /gene="hprK"
                     /locus_tag="ACG8E6_000895"
     CDS             27519..28457
                     /gene="hprK"
                     /locus_tag="ACG8E6_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFS5061334.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28472..29302
                     /gene="lgt"
                     /locus_tag="ACG8E6_000896"
     CDS             28472..29302
                     /gene="lgt"
                     /locus_tag="ACG8E6_000896"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS5061335.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGSLPDYDRSLGKNQQII
                     "
     gene            29346..30371
                     /locus_tag="ACG8E6_000897"
     CDS             29346..30371
                     /locus_tag="ACG8E6_000897"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS5061336.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTNV
                     KAAKYVQELFMNHYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30386..31324
                     /gene="galU"
                     /locus_tag="ACG8E6_000898"
     CDS             30386..31324
                     /gene="galU"
                     /locus_tag="ACG8E6_000898"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS5061337.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            31449..31898
                     /locus_tag="ACG8E6_000899"
     CDS             31449..31898
                     /locus_tag="ACG8E6_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFS5061338.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFLKTKNILKRQEENKNG"
     gene            31891..32505
                     /locus_tag="ACG8E6_000900"
     CDS             31891..32505
                     /locus_tag="ACG8E6_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS5061339.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(32568..33671)
                     /locus_tag="ACG8E6_000901"
     CDS             complement(32568..33671)
                     /locus_tag="ACG8E6_000901"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFS5061340.1"
                     /translation="MNNVKINELRQWMAQHQLELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            33881..34882
                     /gene="ccpA"
                     /locus_tag="ACG8E6_000902"
     CDS             33881..34882
                     /gene="ccpA"
                     /locus_tag="ACG8E6_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFS5061341.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            35236..35631
                     /locus_tag="ACG8E6_000903"
     CDS             35236..35631
                     /locus_tag="ACG8E6_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061342.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREAQKQQDLLAVEKQS
                     VKVLKNTFADIKEVKIEQFEKNDMTGIYEALITMTNTEGKAVYFDYSFTKESNEIDGY
                     GVENEEVQKEGITVDEIIVIYTDGSEEKV"
     gene            35628..36884
                     /locus_tag="ACG8E6_000904"
     CDS             35628..36884
                     /locus_tag="ACG8E6_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HFS5061343.1"
                     /translation="MTITDKQYKGISNKVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKNSFDGMQAMAVAPIKNGKVDISQVVIAFAGTNLSDWKDLETDARSVMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAYLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSVKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            36896..37219
                     /locus_tag="ACG8E6_000905"
     CDS             36896..37219
                     /locus_tag="ACG8E6_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061344.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            37212..>37452
                     /locus_tag="ACG8E6_000906"
     CDS             37212..>37452
                     /locus_tag="ACG8E6_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061345.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQTWAEKRLTDQNHQ
                     MTAEEATQEFSNRHAHLKESVKGQFAEKLTELFQRQ"
BASE COUNT        12532 a         6447 c         8117 g        10356 t
ORIGIN      
        1 ttgtctcagt cccttttttt gcatattttt agcatttatt tcctaaaaaa tgtaagggga
       61 ttcctatatt tttaagtata cttgtttttc cttttataca ttttatggta ttatcatttt
      121 attagttatt ataaaacaat cgttagaaag gacgtcatta tgcaagtata cggaattgat
      181 gcggtgcttc gaatcgtgag tcatttggcg tttatctact tagccttctg gtctttacgt
      241 tcgttacgca ttgaaacttt tttcagacca atgcataata cgcaaattag aatggcaatc
      301 gtactttttt caattttttt agggtacact gcaagtaatt ttttcttaga agttatcgct
      361 ttatgccgaa atcttttcgt tagcctgtaa attttaaaaa aaggtactat tttaaaagca
      421 aggatgttat aatagaaaag attttgagta agtgtatgct cattggaatt ttttggaggg
      481 aacaacatgg aagagatcat cgtaagaggt ggcaatcaat taaatggaac agtaagaatt
      541 gaaggagcta aaaatgctgt tttgcctatt ttagctgcta gtttgttagc agaagaaggt
      601 attacgacat tagacaacgt tccgattctt tctgatgtct tcactatgaa tcaagtgatt
      661 cgtcacttaa acgtagatgt agattttgat gaacaaaaga atcaagtaac aatcgatgct
      721 tcaagacaat tagaaatcga agcgccttat gagtatgtta gtcaaatgcg tgcttcaatc
      781 gttgtgatgg gtccgctact tgccagaaat ggacatgcaa aagtagctat gcctggtgga
      841 tgtgcgattg gtaaacgacc aatcgatttg catttaaaag gatttcaagc tttaggagca
      901 aaaattattc aaaaaaacgg ctatatcgaa gcaattgcgg atgaattgat cggtaacacg
      961 atttatttag atttccctag tgtaggtgct actcaaaata tcatgatggc tgccgtaaaa
     1021 gcaaaaggaa caaccatcat tgaaaacgta gctcgcgaac cagaaatcgt tgatttagca
     1081 aacattttga ataaaatggg cgctcaagtc tatggagctg gtactgaaac aatgcgtatt
     1141 gaaggcgtgg atcatttaca tgcagtcaac cactcaatcg tacaagatcg tatcgaagca
     1201 ggtaccttta tggttgctgc tgcaatgaca caaggcaatg tgctgattgc agatgctatc
     1261 tctgaacata accgcccatt gatttctaaa ttgatcgaaa tgggtgcaga aatcattgaa
     1321 gaagaaggcg gagtccgtgt catcggacca aaacatatct tacctacaga tgtaaaaacg
     1381 atgcctcatc ctggtttccc tacagatatg caagctcaaa tgacagctat ccaattagtt
     1441 gcagaaggta caagtgtagt gactgaaaca gtgtttgaaa atcgtttcca acatttagaa
     1501 gagatgcgcc gtatgaacgc tcatgtcaaa atcgacggaa atgtagcaat catggatgga
     1561 aaccatgaat tgcaaggtgc agaagtatat gcaacagact tgcgggcagc agcagcatta
     1621 gttttagctg gcttgaaagc aaatggaatc acacgtgtgc gtaacttgaa ttatctagac
     1681 cgtggatatt ataatttcca tatcaaacta cagcaattag gtgcagacgt tgaacgtgta
     1741 gacatggatc aaacttctgc tgaaaaaaca gcacaaacga ttgcttaagg aagtggctga
     1801 atgagtaacc aacgatatat catattaact ttgataaaga ttttagtggt gatcttattg
     1861 cttatccttc tttttgtggc cggaacgatg atcggatatg gtgtcatcgg tggcggcaat
     1921 ccatttaaag tattccagcc aagtttgtgg attcatattc gagacttttt tcattgataa
     1981 ataagaatca cagtggttct agtcgatatg aagtgagcca tgaagcagaa catgacaaaa
     2041 gaaaacagaa gggaacgttg acgttagtcg cgatcccttt ttttagtaaa agaaaagaag
     2101 gaatctaatt gatgaagaag atattgattt atttagttag aggatatcag cgctttattt
     2161 cgcctgcatt tccacctagc tgccgttatt acccaacctg ttccaattat atgataaaag
     2221 caattcaaac ccatggtgca ctaaaaggga cggtaatggg gactgcacgg atcttacgct
     2281 gtcatccttt tgtcaaaggc ggtatcgatt atgtaccatt gacattccaa ttaacgcgaa
     2341 ataagaaaga taaaaaagaa ccagattata aaaaggttgt gaaaaatacg agtagcactg
     2401 agcgataaag aaacaatttg gaaaatagct tcttatattt tgcaaaattg tgagttattg
     2461 ccgaagtgtt ggattttgaa caccgtcaaa aaaaggttgt gaaaaatacg ggtagcactg
     2521 agcgataaag aaaaagagga aaattattgt caaagtaaca actccgcaac tacttatatg
     2581 ggggacaaaa aatgatcgaa gaactaaaca agaaagattt accatggtcc ttgttattag
     2641 atgcagatcc tgataaagaa aaagttcaag tatatgtttc cagagggagt ggctggatat
     2701 ggaaagaaaa agaaaaaaca ataggtgtac tgatctatgt ggcaagggaa aaagaatacg
     2761 aaatcgtcaa tgtagcagta gccccaacgt atcaaggaaa ggggattggc ggaaaattac
     2821 ttgagacagc ttttcagaag ctttcacaac tcgcttccag tcaaactcag atcatcatac
     2881 gaaccggcag tactagcagt gcggcgttac atctctatca aaaaatgggc tatgtggaga
     2941 ttggtcgagt caaagactat tttatccatc attactctga gcccattttt gaaaatggag
     3001 atcaattaag ggatcaagta attttagcaa ttcacttacc taaaaatagc tttaactaat
     3061 aaactttcaa ttcgagaaaa tgctttcaaa tgctaaatag atacattaca atagctttag
     3121 caaacaaaaa ggagaatatt tttatgggaa tattagtttt tgattttggt ggatcggctg
     3181 tcaaatatgg ttgttgggat ggcaaagaga ttaaaggtaa gggacaattt gcaacgcctg
     3241 aatcttggga agaaatgaaa gcgcaattat ttcaagttta taaaaaaata ctcgtttctt
     3301 ttgaagtcga aggaattgga atcagctctc ctggagtagt gaataatgag aaacaggtga
     3361 ttgagggaat cagtgcgatt ccttatattc atggatttaa tattttccga gatttggaaa
     3421 ctctgtttca actcccagta acgattgaaa atgacgccaa ctgtgccgga atggctgaat
     3481 tttatgaagg tgctgcccgt gattatcaag atgttgcttt tgttgtggca ggaactggga
     3541 ttggcggggc gttgttcacg aaaggacaaa tcaataaagg ggcacatctt tatggtggag
     3601 aatttgggtt gatgttctta aatggcgata agacattcag taaacttggt acagcggtac
     3661 agatggcttg gcgttattgt gaacgcaaag gtctcgataa aaattcttat accggaaaag
     3721 atgtctttga attagcagaa actggagatg caatagcaaa agaagaagtt gagagttttt
     3781 atacgtatct tacaaaggga ttattcagta ttcaattttc gtttgatcca gaagtgatcg
     3841 tattaggcgg cggtgtttct gcaaaagaag ggctcattga tgagatcaaa agccgaatga
     3901 agaagctgac agaacaattc gatctgcatg atttcgaacc gcagatttta ttatgtgaat
     3961 acagaaacga tgctaatctt gtaggtgcag cagcaaacta tctcgctgta cgtcaagggg
     4021 agaattaaga gctttagaga aggtgcagct tgcataaaag aagtgaatga aatgaatagg
     4081 agatttttac ctaaggaatg ttttatttgc tgcagtatga taaaatatct gagaggtgaa
     4141 aatgatgtca aaaaaaaata aagatgttga agtgcgtata gaagaagtga aaaaagatat
     4201 caaaggtaag aattatgaag tcactcagtt atttataggg aaaaaaatga ttggtgagat
     4261 cttagcctat gggccaaaag aatatgaaat tttctttggt gaagaagatt ttggaaaaga
     4321 aaaaagtctt gaaaatgcga tagaaacagt gattcgacac tggaatctgc acgaataaaa
     4381 aaatatcaaa aaaactttca ataaagggtt gcaaaaatct ggaaaattag gtattataac
     4441 taagtcaggt tgatacaaac ctttcggagg agtagcgaag tggctaaacg cgacggactg
     4501 taaatccgtt ccttagggtt cagtggttcg aatccactct cctccatttc attggggtat
     4561 agccaagcgg taaggcaagg gactttgact ccctcatgcg ttggttcgaa tccagctacc
     4621 ccagtatacc acaatcgtaa gctctcgtta gttcgagagc tctttttttt gctttgctac
     4681 cttgttcctt gaagattctg ctgatttaag atagagttaa gttaactaca agaattggag
     4741 ggatccacat gaaatggaaa gaaaaagtag acgaagcagc tgaaaagtta tatgatttgg
     4801 tcaaatcaga aaaatacaat attgaagtga atattccgaa aaaaggcgga aaagcggtcc
     4861 aggtaaaatc aaaacgccca acgaatcata caaaaaaatg gatggctaaa aaccgctaat
     4921 aataacaaaa gatccataag tcggcatttt catcgatttt agggtctttt gttattttct
     4981 aggttgaaca tatctaaaaa attgtttctg acaataggag taggaagaac gatgtgtgtc
     5041 taaatcctct gttgatggat agaaaccttt atctttttca gttgtgaatc aggtatatac
     5101 cagttataat agaagaaacg aaaggacggg tgtacgttat gtcgaatcaa ttacctgtat
     5161 atatacagat tcatgaccaa ttaaaaaatg aaatcgaaca aggtatatgg aaagtcggag
     5221 accgtcttcc ttcagaaaga gaattagcag tgaaattcaa tgtcagccgt atgacgttga
     5281 gacaagcaat tcaaaacttg gcagatgaag gaatcttaga aagaaagatc ggttcgggta
     5341 catatgttgc gcgagaaaaa gtacaagaaa aaatgtctgg tgcaacaagt tttacagaaa
     5401 ttatggaatc gcaaaatcga gtaccgtcta gtcggacaat ctcttatttc ttgacttcgc
     5461 caagctcaag tgaaatggaa aaactaaact tgagtaaaga tgacacgatc ttgcgaatgg
     5521 aacgtattcg tttcgcagat gatgtcccta tttgttttga agttgccagt gtgccacaaa
     5581 agctgatcga tggctacagc aaagcagaga ttactgaatc gttgtatcgc acgctagaag
     5641 aaaaaggcgg gaaaaagatc ggtccagcga atcaaacgat tactgctgtg ttggcttctg
     5701 agaaaattgc tgactattta gaggtaaaac gaggagatgc agttcttcgt ttgcgccaga
     5761 tttcttattt tgaagacggt acgccgtttg agtatgtgcg tacgcaatac gtaggaaatc
     5821 gttttgagtt ttatttggaa aaataaaaaa caggtgatcc ctgattcctt acttagaaat
     5881 aaaagagaat cagaatcacc tgtttttata tatgatcagt taccagttgc tcgtgataag
     5941 aaacaggacc ctcttcaaat tggacttgtc cgttcagcag ctcaatgatg gattctttaa
     6001 agtggtcagc acttttttca tctacagcac agatgacgct aacttgatca gtatattgga
     6061 tttcttttat aaaaaaatgt tggagttcaa gataattctg taattttcct agttgcggat
     6121 acgtgatcat cacagagatc tcctgttgca aggtaccttc tacgattcca atgtcagaca
     6181 atgcttgaga gacagcatga gtgtatgctc gtattaatcc gcctgtgccg agtttcgttc
     6241 ctccaaaata tcgagtgact actgcagcta cattgatcag ttgatttttt ttgaggactt
     6301 ccaacatcgg tacgccagcc gttccacttg gttctccatc atcgctgctt cgctggattt
     6361 catttttttc tccaatgatg aaggcgctgc agttatggtt cgctttccaa tgttcttttt
     6421 ttatttcttg aatgaaactt tttgcttggt cctctgattc tattcgtcta agagaacaga
     6481 taaagcgtga ttttttgatc tcaatctcac tttgtccatt ttcttttatt gtacgatagc
     6541 gctcgatcat cttgtcacct ctttttttca ttttctttat tcagtataaa taaagttcta
     6601 ggttgtttca acactgtaac ggttgcgcaa tcgctgtagt atgcgataat aaagacaaag
     6661 gtaggtggat ctggggatgc aggaacgaat gaaaaagtac gatgcaatta cgcattattt
     6721 gaagaacaat ggcggctctc aagtaacgtt gacctttacc caatttgacg aactgctgtt
     6781 tcccagtaac ggacttccca aaacagcaag ggaaagtaca gattggtggg caaatgatta
     6841 taaacaccct gaaaaaggtg cctatggctg gataaatgca ggctatgaag tagttgtcat
     6901 caatttggac aaagaatatg tcgtgttcaa taaattagtg aaatctagtt ggctatttga
     6961 ttaaataaac cgcgtttaga aaacgttgac aaatttcttt ctttttatat aatagagtaa
     7021 cagacgcgaa gaaaacgttt gcgtagtttg ttttaaaaag gagagaaaag tatggctaaa
     7081 aaaacaatca tgcttgtatg ttcggctggt atgagcacaa gtttactagt aacaaaaatg
     7141 caaaaagctg ctgaagcaaa aggaatcgac tcggatattt tcgctgtgtc cgcttctgac
     7201 gcagataaca atcttgcaaa taaagatgta gatgtactgt tattaggacc tcaagttcgc
     7261 tttatgaaag ctgattttga aaaacgtctg gaaccaaaag gcattccttt agacgtcatc
     7321 aatatggctg attatggtat gatgaatggc gaaaaagtgt tagatcaagc aatcacgctc
     7381 ataggtgaat aaatagaaca gtataactat cgaacaagcg atccaaaaaa tagatcgctt
     7441 gttttttcgt atattttttt agggcgaata atatgaatca tttttggggt agaaggcttc
     7501 tgacgagaga tattaaagca atagactgtg aggaggtaaa tttaccaaat tatgagaaga
     7561 tcgcagcagt ggaacgtttg ggcaactgga ttctttgtca tcgctgtgga atgaggacgt
     7621 ccgtcattga tggacagctt gcagattacg gttatttttg tacacattgt ctatatttag
     7681 ggcgatgcga cactcaacaa gatttatatt tgtttgatca gcctgaagca aaacctcgag
     7741 aagtcgtctt tttttggaca ggacagctta ctgaaaaaca aacggaaatc gcaaagagaa
     7801 tactttgcca ttcagaaaaa agacatcatc ttatttgggc tgtaacaggt gcagggaaaa
     7861 cagagatgct ttatcctatt ttagtggaaa ctttaaaagc tggaggaaga gtagcgattt
     7921 gtacaccgcg agtcgatgtt tgtaatgaat tgtttttacg ctatcgttat gtttttccag
     7981 aagaaaaaat cacgttgctc catgggaata cagcaacggc ttataccttt acttcttttg
     8041 ttatctgtac gattcatcag ctataccgtt tttatcggtc ctttgattta atagtgatcg
     8101 atgagatcga tgcgtttcct tataacgggg atgcaggatt agctcatgct gtgcaaacag
     8161 cgattaaacc agacggacga ttcatctact taagtgccac acctgataaa aagttactaa
     8221 aggaaatcaa gcagacgttt gacatccaca aacttgcttt gcgctttcat cggcgtttac
     8281 ttcctgaacc cgtcttggtt ttcatgaata attggcgaca aaaatgcaag aacaagaaga
     8341 agataaaatc tttggtgcgg atcattcatc aattaattaa agaaaaccat atccttttat
     8401 tttgtccaag tatcacgatg atgcatcgtt tgaaagccac acttcaagag gctcttccga
     8461 gatatgagat cacagatgtt tcttcaagag acaatcagcg ggcggaaaaa gtccagaaaa
     8521 tgcgagaagc aaaatatgac atactgttaa ccacgatgat ccttgaacgt ggagtcacat
     8581 ttgaaagaat atccgttatc gttttaggag cggatcatcc ggtttttact aaatcttctt
     8641 tggtacaaat cgcaggaaga gccgatcgta aagggcctta tacgaacagt caagtatact
     8701 ttttctatga agaaaaaact gcagctattc gaaaagcttg tcaggaaatc aaagaaatga
     8761 atgaagaagg gaagaagatg ctgtgaggtg cagttgctgc agtaaagata tcgtccaaaa
     8821 tttaacattc agcgagatta ttggtgtttc gaaatacaga ttacctcaga cttgttcagt
     8881 ttgtcgcgca caatttcatt tattggaaac aagaggctgt tctggctgtc aaaaaccaat
     8941 ccatttcaat ctagagagta cgaaaaagaa agtaaagtct attcccgttt actgcagtga
     9001 ctgtcaagaa tggaagaaga aatatccgac ctatgatttt cagcaccgtg ccttttttgt
     9061 gtacgatgaa aatatgaagg cctggctcaa tcaatacaag tttatgggag atatccgact
     9121 agcaggcacc tttgcatata aatggacgca gataaaaaaa gaatacccga catacatcta
     9181 ttgtccaata cctttatctg aaaaaaggcg aacagaaaga ggattcaatc aagtaagcga
     9241 gatgttgaaa gcagcaagaa ttccttttca gatgctactc aaaagagaga agcatctgct
     9301 ggctcaagca aaaaacacgc gaaaggaaag attggagatg cctcaacctt ttgaacttgc
     9361 agtcaccttt tctaagatac aaaataaaaa aatattgatt attgatgatg tctatacaac
     9421 aggacagact cttttccacg cagcagattg tttgcaggtc gcacagccga aaatcatccg
     9481 aaccatttct ttagcaagat gaattagatg tggaaatttg atagcgattt aatttgcaaa
     9541 taacctagag ttcgatataa tagaattaag aagagggaaa gagtggtcct tctccttctt
     9601 gattgtggta gacgaaaggg gtaattttta tgtttagata taatgtacgc ggcgaaaata
     9661 tcgaagtaac tgaagcgatt cgcgactacg ttgagaaaaa agtaggcaaa ctagaacgct
     9721 atttcagtga cgcacccgat gcaacggcgc atgtgaactt aaaagtttat acagaaaaaa
     9781 cagcaaaagt ggaagtaacg atcccattac cttatcttgt attacgtgcc gaagaaactt
     9841 ctccagattt atacgcaagt atcgatttgg ttgttgacaa attggaacgc caaatccgca
     9901 aattcaaaac aaaaattaat cgtaaatccc gtgaaacggg catgaatact gcagatgcta
     9961 ctgctttatt caatgaagag gacgaaaacg aaaatgattc tgaattagat atcgtacgta
    10021 cgaaacgcct ttcattgaaa ccaatggata gtgaagaagc agtcttacaa atgaacatgc
    10081 tgggacataa cttctttatt tttgaagatg cagaaacaaa tggtacaagt atcgtttacc
    10141 gccgtaaaga tggaaaatac ggactgattg aaacagactg atcagcctta cttaaataac
    10201 aacgaaaact tgcagcagct actgacagct gccgcaagtt tttttgagtt tcctcctctt
    10261 ttagatgaaa tatgaataaa ttatgaagtt cttactttaa tacgcttaag atatttcaat
    10321 tatctataac taatgataaa atgaaaagta aagtctacat agtctggata atacacagaa
    10381 ggaaaaggag attacacaac gcatggccaa tttgattcgt tcacttatcg aaaacgataa
    10441 gaaagaacta aaacgtttag atagcattgc aaaaaaagtt gaagcatacg ctgaccaaat
    10501 ggcagcatta actgatgaac aattacaggc aaaaactgct gaattcaagg gcagatacca
    10561 aaaaggagaa acattagatc aattacttcc ggaagctttt gcagtcgtcc gagaagcagc
    10621 aaaacgtgta ttggggttat atccatacca cgtacaatta atgggtggga tcgtgcttca
    10681 cgatgggaat atccctgaaa tgcgtactgg tgaaggtaaa accttgactg caacgatgcc
    10741 ggtgtatctg aatgcgttga ctggcgaagg ggtccacgta gttaccgtca atgaatattt
    10801 agcaacccgt gactcgacgg aaatgggcga gctttataat ttcttaggat tgacagttgg
    10861 gttgaatatc aactcaaaat cttcggatga aaaacgcgag gcttataatt gcgatatcac
    10921 ttatagcacg aataatgaat taggatttga ctatttgaga gacaacatgg tcgtttaccg
    10981 tcatcaaatg gtacaacgtc cgctgaatta tgccattgtc gatgaagttg actcgatctt
    11041 gattgatgaa gcgagaacgc cgctgatcat ttcagggcaa gcagaaaaat caacagcttt
    11101 atatacaaga gcagacaact tcgtgaaacg tttgaaagaa gaagaagatt ataaaatcga
    11161 tatacaatca aaaacaatca gtttaactga agcagggatc gaaaaagctg aagaaacatt
    11221 cggtttggac aacttatatg acattgaaaa tactgcattg acccatcact tggatcaagc
    11281 attgagagcc aactacatca tgcttttaga catcgattat gttgttcaag atggaaaagt
    11341 attgattgtt gaccaattta cgggtcggat catggatgga cgccgttatt cagatggtct
    11401 tcatcaagcg atcgaagcta aagaaggcgt agagatcgaa gacgaaacga aaacgatggc
    11461 gacgatcact ttccagaact atttccgtat gtacaagaaa cttgcgggga tgactggtac
    11521 agctaagaca gaagaagaag agttcagaga gatttataat attcaagtat accagattcc
    11581 gacaaaccgt ccggtcatcc gtgacgatcg accagatttg ctttacccaa cattacaaag
    11641 caagttccat gctgtcgtac aagatatcaa agatcgctac cgcaaaggac agcctgtctt
    11701 agtcggaact gttgcagttg aaacatctga gttattatca gatatgctga atcaagaacg
    11761 tattcctcat gaagtactga atgcgaaaaa tcactttaaa gaagcagaaa tcatcatgaa
    11821 tgcgggtcaa aaaggtgcag tgacgattgc taccaacatg gctggtcggg gaacagatat
    11881 caagctaggg cttggcgtaa gagaacttgg tggcctagca gttatcggga cagaacgaca
    11941 tgaatctcgt cgtatcgata accagcttcg aggacgtgca ggacgtcaag gagaccctgg
    12001 tatgtcacaa ttctacttgt ctttagaaga tgatttgatg aaacgattcg gttcagaacg
    12061 aatcaaagct ttcttagatc gaatgaagat cggtgacgaa gatgctgtga tccaaagcaa
    12121 gatgctgacg aaacaagtag aagctgcaca aaaacgcgta gaaggaaaca actacgacac
    12181 acgtaagaac gtcttgcaat acgatgacgt gatgcgtgag caacgtgaag taatctataa
    12241 acagcgtcaa gaagtgatca tggaagaaaa aagcttatct aaaccactga tgaatatggt
    12301 caaacggaca atcagtcgtg tggtagatgc gcatacgcaa ttagaaaaag aaaattggaa
    12361 tttggaaagt atcgtcgatt ttgctggcag cacgcttgtc catgaagaca gtatttccct
    12421 tacagatatc gaaggaaaaa cctctcaaga aatcaaagaa tatctaaacg aacgagcaca
    12481 agaagtattc aataccaaag cagctcaatt gaacggacca gagcaactgc ttgaatttga
    12541 aaaagtcgtg atcttacgtg tcgtggactc taaatggaca gatcatattg atgcaatgga
    12601 ccaattacgt cagtcaatcg gcttgcgtgc ctatggtcaa aacaatccat tagtcgaata
    12661 ccaaacagaa ggttataaga tgttcgaaga tatggtaggt gccattgaat atgaagtcac
    12721 tcgtatcttc atgaaagctg agatccgtca aaacgtacaa cgtgagcaag tttctcaagg
    12781 aacagcttct cactcagaag atggagatgc caaagaaaac agtaatacta gcgcgaaaaa
    12841 acaaccaatc catgtgaaca aggttggtag aaatgatcct tgtccttgtg gaagcgggaa
    12901 aaaatataag aactgtcacg gaaaagatgc aaactaattt ctgacagcag gattggtgag
    12961 acaaattctt ttttaaagaa tttgtctcac cttctttctg ttatttgacg attctgttag
    13021 actagatata ataaaaaaag tatagtgaaa gaaggaaaaa aacatggaaa tatccgaaat
    13081 tcgaaatctc ctgacagaaa tgcaggaaaa ggtcacaagc ttcaggaggt ctctttgact
    13141 tagaccagct ggaagaagac attgcggaag cagaagcaaa aatggctgaa cctggttttt
    13201 gggatgatag cgaagctgct caaaaagtga tcagtgaaaa caatgcaaat aaagaaactt
    13261 atgatcaatt ccattctctt gctgaagaac tggaagaaat cgaattgatg gtggagatgt
    13321 tgcaggaaga acctgatgca gtaatgcaag aagaagcaga agcacgtatc aatgcgctag
    13381 atgaaaaaat gaatacgtat gaattatcta tgttgttaga tggtccttat gatcgaaaca
    13441 acgccatcgt cgaattgcat ccaggggctg gcggaacgga gtcccaagac tggggcagca
    13501 tgcttttacg catgtataca cgctgggcag agcagcatgg gtatcaagta gagacactag
    13561 attatcaagc aggcgatgaa gcaggaatca agagtgtgac cctactgatc aaaggacaca
    13621 acgcatttgg ctatctgaaa tcagaaaaag gtgttcatcg ccttgtacgt atctcgcctt
    13681 ttgactcagc aaaacgtcgt catacttctt tttgttcggt agatgtgatg ccagagcttg
    13741 atgaaacgat cgatatagaa atcaatccag atgatttgaa gatcgacaca tatcgggcga
    13801 gtggtgcagg tggtcagcac atcaataaga cagaatccgc tgtacgaatc actcacttgc
    13861 ctacaggaac agtagtagcg agtcaggcac aacgttcaca attgaaaaac cgggaacaag
    13921 cgatggggat gctgaaagct aagctttacc aattagaagt ggaaaagaaa gaacaagaag
    13981 cagctgcact gagaggagaa caaaaagaaa tcggctgggg atcacagatc cgttcttatg
    14041 ttttccatcc atattctatg gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc
    14101 aagctgttat ggacggagat cttgatttat ttattgacgg ctatttgaaa atgcatctgc
    14161 aataagagcc ctttgttaaa ttgaaataaa attgtaagat tttgcaagca agttgaaaca
    14221 ttgccatgct ataatagcta aagactgaaa aagatatgga gagaacgcca tgattgaaat
    14281 gaaggatgta atgaagaagt actcaaacgg taccactgct atccgcaatc tctcgattgt
    14341 catcgaacaa ggagagtttg tgtatgttgt tggtccgtct ggagctggta aatctacatt
    14401 catcaaattg atgtatcgag aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca
    14461 tgatctaata gccatcaaaa accgcgaagt tccttatcta cgcagagaaa tcggcgtggt
    14521 gttccaagac tataaattat taccaagaaa aacagtatat gaaaatgttg cgtacgccat
    14581 gcaagttatc ggcagaaaac ctcgggatat caaaaaacga gtaatggaag tgctggatct
    14641 tgttggtttg aaacacaaag tccgagtctt tcctagcgaa ttatctggtg gggaacaaca
    14701 acgtgtttct attgcccgag caatcgtaaa tacacctaaa gtacttatcg cggatgaacc
    14761 gacagggaat ctagatccgg aaaactcatg ggaaatcatg aaactattag atcggatcaa
    14821 tgcacaagga acaactgtcg tgatggctac acataacagt acgatcgtaa atacgattcg
    14881 acaccgagta attgctatcg aaaacggccg cattattcga gaccaagcgg aaggggaata
    14941 tggatacgat gattagaacc tttttccgcc atttattaga aagtatcaag agtttgaaac
    15001 gtaacggctg gatgacagta gcttcggtca gtgcggtaac gatcacgttg actttagttg
    15061 gtgtgttcat ggcggtcatc atgaacgcaa ccaaactagc acaagatata gaaggaaacg
    15121 tggatgtatc tgtgttcgta gacattggta caaaagacga agagatgaaa aaattagaaa
    15181 aagaattaaa tgacttagat catgtcaaaa aagtcgaatt ttcaagcaaa gaacaggaat
    15241 tgaagaaaat ccaaaacgaa atgggcgatt cttggaaatt gtttgaaggg gataataacc
    15301 cgttatatga tgtatacgta gtaagtgcta ccgatccttc ttatacaaaa caagtagcag
    15361 aaaaagcggc agatttgaag aatgtttccc gttctgatta cggtggggct tcctcagatc
    15421 ggattttcca aattgctggt gcagttcgaa catggggatt agttgcggcg gcattgctgc
    15481 tgtttgtagc aatgtttctc atttctaaca cgattcggat cacgatttta tctagacagc
    15541 gtgaaattca aatcatgcgt ttagtagggg caaaaaatgg atatatccgc tggccatttt
    15601 tccttgaagg tggttggatc ggcttaatcg gtgccattct tccgatactg atcattacat
    15661 ttggttacgc atggttttac caattgatca atccgtcatt attacgatct cattattcat
    15721 tgatccatcc agaaaatatc gtttggaaaa tcaatctttt gatggtcggt attggtgtga
    15781 tcattggttc actcggctcg atcatctcta tgagacgttt cttgaaattc tagtgtcaca
    15841 agataaatat aatcagaaag ttggacgatt tcgttccagc tttctttttt tatcagctag
    15901 gtggagtaaa tggggcggct tttttctctt tactttaaaa aacaacttgg gtattcttaa
    15961 gttggtagaa aaagaggtga agccgtgaag attatcgttg acaatttttt tacgatcctt
    16021 ttgattatga atattttgct ttcctttatt atcgtttttc gagaaagaaa ggaaactgcg
    16081 caaacatggg catggctgct cgttttgatg ttcattccag ttgtaggatt tattttatat
    16141 atttttttag gaagagggat ttccaaagag aaaatctttg atttgaagat ccaagggaaa
    16201 atcggtaaga atctagaaat cgaagaagat aaacaagcat tgatgcgagg gctttatcct
    16261 catccgccta ctggacaagt agatgtgaaa caattgatct atatgcttac tgtatttgaa
    16321 agtacgcttt atacgactaa aaatgaaatc actttattta cagatggtcg tgaaaaattc
    16381 gatgcgctga tcgaggatat caaacaggca gaagaccata tccatttcca atattatatt
    16441 taccgcagtg atacattagg tgaagaagtt cgagaggcat tgacagaggc agcaagacgc
    16501 ggagtaaaag tcagagtcct tcttgatgca tggggttcca cacaagtttc ttctagtttt
    16561 tttcagaatt tgaaaaaagc aggtggagaa gtcgcatttt tctttcctct attcgttccg
    16621 tatatcaatc ctcgaatcaa ttatcgcaat catcgtaaaa tcgtagtgat tgatgggaaa
    16681 atcggatata caggtgggtt caatgttgga aacgaatatc taggattggt aaagaaattt
    16741 ggttattggc gagacaatca tctgcgtatc tatggagaag cggtttacac cttgcaaaac
    16801 cgttttttga tggactggaa ttctcagcat acaaaagaag taggatattc accggaattt
    16861 ttcccgacca ttcattctac aggtgatatg gctgttcaaa tcgtgacaag cggaccagat
    16921 accgaacatg agcaaatcaa aatgacatat ttgaaaatga tttcgttagc caaacgggag
    16981 atattgatcc aaacgccgta ttacataccg gatggatcga ttcatgaggc attgaagttg
    17041 gctcttctct caggcgtaaa agtccatatc caaataccta acaaaccaga tcatcttctc
    17101 gtttactggg caacgtattc tttcgctgca gagctgatcg agtatggggc aagaatcgaa
    17161 acatatgaaa atggatttat ccatgcaaag acgatgatta tcgatggcgg catcgtttcc
    17221 gttggttcag ctaatatcga cgttcgctca tttagattgg attttgaagt caatacactg
    17281 atctatgatg aacgaatggc ttctcgcgta agaaaagcat tctttgaaga ttcaaagatt
    17341 tcgacgcatc tcacaaaaga agtgtatgaa aaccgaggga tgatgatcaa gatgaaagaa
    17401 ggattagcaa gactgatttc gccgctcctt taaaatacaa aaaggacagg agagccatca
    17461 atagatggaa atctcgcgtc cttttttctc ggggccattt ctgagagtac gtcagaaatg
    17521 gagaaagcgt ctcgcatgag cgaaattaac tttggggtat ctcttgtttt ttggggaaag
    17581 caagaaataa gctaatatat gctattaaaa gagacacatt tcaaagccag tatagcatag
    17641 aaacttttaa tgagatagct ttttttgcaa ttatgagaat ttattttgtt atttaatgat
    17701 tttgattcgt tttcatcaag taatgaaaat gatagaataa acatacttta ggattaaagg
    17761 agatttctaa tgaaaaaact aaaattattc ttaccaataa taggattagc tattctttta
    17821 tcaggctgtt caaagtggat tgatcgagga gaatccatta ctgcagttgg ctcttctgcc
    17881 ttacagcctt tagtagaaac ggttgctgaa acctaccagg cagaaaatcc tggaaaattc
    17941 atcaatgtac aagggggcgg aagcggaact gggttgtctc aagtacagtc aggagcagtc
    18001 gatatcggaa attctgattt gttcgcggaa gaaaaatcag ggattgatgc ttcggcatta
    18061 gtcgatcatc gagtagcagt tgttggaatc actcctattg tgaacaagga tattggcgta
    18121 aaagatatca gtatggaaaa cctgaaaaaa ttatttacgg gcgagatcaa aaactggagt
    18181 gaagtcggtg ggaaagatca gccagtagtt atattgaacc gtgcatcagg aagcgggacc
    18241 cgtagtacgt ttgaaaaatg ggtattggac ggagaaacag ctgttcaggc acaagaacaa
    18301 gattcaagcg gaatggtgcg ccaaatcgtt gcggacacac ccggagcaat cagctatgtt
    18361 gctttttctt acgtttccga tgatgtggat acgttgaacg ttaacggtgt ggaaccaaca
    18421 gatgaaaatg tgacaacgaa caaatggacg atttgggcat atgagcatat gtacacaaga
    18481 gaccatccaa aaaaactgac aaaagaattt ttagactata tcttatctga tgaagtacaa
    18541 gataatattg tcggagagct aggctatatc cctgtttcac atatgaaagt tgaacgtgat
    18601 tggcaaggaa acattgttaa agagtaatct ttacacaaaa tttacaaact tagctaaact
    18661 tttacatgtt attaaaacca aattgatgtt caatttgtac actgagtgag tatgttaaac
    18721 ctgattaagg gggagtcatc tgtggaagat gtgaaaaaag tattattaac aaaatcaaaa
    18781 cgttcaaaaa tggaacagcg cggaaaattc attagttttt tgtgtatcgc gctgatcgtg
    18841 ttggttgtat tatctatttt ttattttgtc gctagtaaag gattggccac cttttttact
    18901 gataagatca atgtatttga ttttctgttt ggtacagatt ggaatccaag ccaaatagga
    18961 gcaaatggga aacccatggt aggggcattg cctatgatca caggttcatt tattgtgacc
    19021 tttttatctg ctattgttgc cacaccattt gctattggag cagcagtgtt catgacggag
    19081 atttctccta aaaacggaac gaagatcttg caaccggtca tcgagctact tgtaggtatt
    19141 ccgtctgtcg tttacggatt tatcggttta tctgttatcg tgccttttgt cagaagtatc
    19201 tttggcggga cagggttcgg tatcttagct ggtacatttg tgttattcgt tatgatctta
    19261 ccgactgtca caacgatgac tgtggatgca ttaaaggcag tgccgcgaca ctatcgggaa
    19321 gcttcattag cgctaggtgc cacaagatgg caaacaatct acaaagtggt gctgcgagca
    19381 gcagtgcccg gaattttaac agcagtcgtt tttggtatgg ctcgtgcgtt tggcgaagcc
    19441 ttagctatcc aaatggttat tggtaatgcg gcattaatgc caacaggact tactactcct
    19501 gcttctacat tgacgtccat cttgacaatg ggaatcggaa atacaatcat gggaacagtt
    19561 gaaaataatg tgttatggtc attagcgctg atcttgctat tgatgtcatt attctttaat
    19621 atcgtgatcc gtatgattgg gaagaaagga gagctgaaat aatgaacgca aaacgctcag
    19681 ataaaattgc tacaggtgtt ctatatgtgg tttcagggat catcgtattg atcttagcag
    19741 ctttattact ttatatctta gttcgaggaa taccgcacat ttcatggtca ttcttgactg
    19801 aaccttccaa agcctaccaa gaaggcggtg ggatcgggat tcaattattt aactcgattt
    19861 atttgctttt gattaccatg atcatcagtt ttcctatctc tttaggtgcg ggtatctacc
    19921 tttcggaata tgccggaaaa aactggctga cagacgtgat ccgtacgtct atcgaaatct
    19981 tgagctcttt gccttctgtt gttgtcggtt tattcggttt cttggttttc gttattcaat
    20041 tccagtatgg tttctcaatc atttctggtg cattagcttt gaccgtattc aacttgcctc
    20101 ttttgactcg aacggtcgag gaatctttac aagcggtcca ttacacacaa cgagaagctg
    20161 gattggcgtt aggtttgtca cgctgggaaa cagtgataaa agtggttgtt ccagaagcga
    20221 caccaggaat tttgactggt gtgatcctaa gttcaggaag aatttttggt gaagctgccg
    20281 cattgattta tacagctggt caaagcgcgc cagcattaga ttttacgaac tggaatccat
    20341 taagtgtttc tagtccgatc agcattttcc gccaagctga aacactggct gtccatattt
    20401 ggaaaatcaa cacagaaggt acgatgcctg atggtgtttc tgtttcggct ggcgcttctg
    20461 ccgtgttgat cattgctgtt ttactgttca acttcggcgc tcgtgcgatt ggcaaacgat
    20521 tataccgaaa aatgacttca gcctaaggag aagaagtaaa atgaaaaaat ataattggaa
    20581 tgaaacaaat gtcattacca tgccaaaaga taaggaagtt gcattatata cgaatgatct
    20641 gcatgtttgg tatggcgaca atgaagcaat caagggagtg gatctagaat tcgaaaaaaa
    20701 taagatcact gcattgattg gtccctctgg ctgcggaaaa tccacgtatc ttcgttcttt
    20761 gaatcgtatg aatgatggga ttgctaatac gaaaattact ggaaagatta tgtataaaga
    20821 cgtggatatc aatgcgcctc aagtggatgt ttatgaaatg cgtaaacgga tcggcatggt
    20881 gttccaacgc cccaaccctt ttagcaaatc gatttatgac aatataactt ttgctttgaa
    20941 acagcatggt gaaaaaaaca aaaagaaatt agatgaaatc gttgaaacaa gcttgaaaca
    21001 agcagctttg tgggatcaag taaaagatag cttggataaa agtgccttgg ctttatctgg
    21061 cggacaacag caacgtttgt gtatcgctcg tgcaatcgcg atgaaacccg atatcttgct
    21121 gttagacgag ccggcaagtg ccttggatcc tatttcgaca ggaacggttg aagaaacact
    21181 tgtaaacttg aaagaagact acacgatcat tatcgtgacg cataatatgc aacaagcagc
    21241 tcgtatcagc gactatactg cctttttcta tctaggaaaa gtaatggaat atgacaaaac
    21301 aaacaagata tttaccagac caaaaattca ggcaaccgaa gattatgtgt ccggtcattt
    21361 tggttaagac ggaggcctca acatgacaaa agaaattatc tcatccaaag atcttcatct
    21421 gtattatgga aagaaagaag cactaaaggg aattgatcta agtttcaatc aaggtgaaat
    21481 cacagcgatg atcggtcctt ctggttgcgg gaaatctacc tatctacgtt cgttgaatcg
    21541 aatgaacgat ctgattccaa atgtaacgat caccggaagt gttttatata aagacaaaga
    21601 catctatggt ccgaaaacag atgttgttga actaagaaaa gaaatcggca tggtgttcca
    21661 acagccaaat ccatttcctt tttctattta tgaaaatgtc atctatggat tgaagttaaa
    21721 aggtgaaaaa gataaaaaag tccttgatca agtagtggaa gaaagtctga aggcagcgtc
    21781 cgtttgggag gatgtgaaag ataaactgca taaaagtgcc ttgtctttat ccggtgggca
    21841 gcagcagcgt gtatgtatcg cacgtgtttt ggcagtgaat cccgaaatca tcttattgga
    21901 cgaaccaaca agtgcacttg accctgtttc tactgggaaa atcgaaagta tgctgttaga
    21961 attaagagag cggtatacga tgatcatcgt gacccataat atgtctcaag cttcacgtat
    22021 ttcagataag acggcattct ttttagatgg tcacttgatc gaatacaacg atacgaagaa
    22081 gatattcatg aatccagaaa aacaagagac agaagattat atttcaggtc gatttggata
    22141 aggaggaaac ggaatgctac gtacacaatt tgaagaagaa ttgctgaatc tccataatca
    22201 gttctacgaa atggggatga tggtgagcag cgctgtgcac aagtcagttc gtgcatacat
    22261 caaacacgat aaaatcttag ctcaagaagt tatcgaaaac gatataaata tcaataatat
    22321 ggaaacgcgt ttggagaaga aaagttttga aatgattgca ttacaacagc cagttacaac
    22381 tgacttgcgt atgattatca cggtcatgaa agctagctct gatttagaac gtatggccga
    22441 tcatgcggtg tccgttgcta aatccacaat ccgcgtaaaa ggacagacga gaataccgga
    22501 aatcgaaaaa gagatctccg atatgtccga ttacgtgaaa aaaatggtgg acaacgtcct
    22561 agttgcgtat gtcaaaacag acgaaaaaga tgcgcgtaca atcgccaata tggatcaaag
    22621 agtcaatgac tatttcaatc gaatctataa tgcaaccatc aaaaacatgc aagaaaatcc
    22681 tgaaacagtc atcagtggca cagactattt gaatgttgcc agctatctag aacgaatcgg
    22741 agactatgtg acaaacatct gtgaatggat cgtctacttg gctacaggga aaatcactga
    22801 attaaatacg aaccacaatg agattttcta aactgaagtt gtgattctgc tgtttattta
    22861 ggaaaagagg ttgtgaaatc agcgttttgt cctgagtatt agagaaacaa acggtaaaat
    22921 agttcctcat attttgccgt tttttgagct aataccgaag gtcagctgat tgaacaccgt
    22981 ttattctgga aaagggtttg tgacaaaaca tgtcacaaac ccttttcatt attccaatag
    23041 aaaggataaa gaatgcaaaa aaagattagc atcatcatca ctctattttt tatttgtata
    23101 cttgcttcat ggtttattca gcgtcaagct gctctatatg acgtacctgt catcgagatc
    23161 gaaagagtga gaatagaaga ggatcaacaa ttgattacag gaagattgat gaatacggac
    23221 caaaaaggag aagtgatttc tttaacggct ccttatcaaa aaaatcaaat cgaaacagca
    23281 aatataacaa caagacaaat ctatcttgtc caaatgcatg gagatgaatg gcaactagta
    23341 gcaaaaaaaa atgatggctg gctttttttc tttacaagtg tcttcattgg caccatgttg
    23401 cttattggtg gaaaacaggg cgtatttgct ttaggaagtc tcatcatcaa catcgtttta
    23461 cttttacttt tgttgctcct ctttaccaaa acagaaggta tttctctctt aaatatagca
    23521 atcttatttg tcgttatagg gataatcgtt agtatattgg cgttagacgg atggaataga
    23581 agcagtataa tcaaaatttc ggctgcagtt acaagtgttt ttttagcatt ttttatttgt
    23641 cttttgacaa tgaatcattg gaaagatcaa ggactgcgct atgaagaact gaattatctg
    23701 acacgaccgt atcgttctgt ttttctttcc agcgtgctga ttgggacagc tggagggaca
    23761 ctcgataccg tgatcacagt cattgcgaca ttagaagaat tgaccaagca acaaccgaaa
    23821 atatcagcga aacagttatg gctttcagga aggcaagtgg gccgagatgt gattggttcg
    23881 atggctaata tccttttatt cgtctatatc agcggagcga ttccatcgct tctgttatat
    23941 ttaggaaatc aatggtcttt caaagaaacg gtagaacgac atttatcatt agaactttta
    24001 cgcgtcattg ctggcagctt aggaatagta ttatcaatcc ccatcgcggt cctctttttt
    24061 ctcgctgtaa ggaggttaaa aaaatgaatg tacttattat acttgttggg atttttgcaa
    24121 tcagtgtgct atttgtcggt ggtacgcaag gtatgtatat tcttttagga ttattcatta
    24181 atttaggaat ctttttccta ttattattcg gctatcatca gaagtggccg atattggtat
    24241 tgtcgattat agggttccta ttgatcgctg tggttatctt attttttatc aatggttata
    24301 atctgaaaat gcgagccgct tttgccagta tactgatctt tttgttttgt ttcctactat
    24361 tgattcctat cactgatttt ttagcgatcc aaggctttac ttcgatagaa ctagaagaac
    24421 tatctggctt agataagacg ttagcaattg attttagact acttacgagg tcgttgcttc
    24481 tgatcagtct tagcggagct gttttagatg cgagtgtagc gatttctagt ggaacattcg
    24541 aagtatatca ggcaaatccg caaatgtctt ttaaccaatt gcgccatgct agctttgcca
    24601 ttgctaaaaa aatattggca tcaacagtga tgacgcttct ttttgcgttt atggggagtt
    24661 ctttagcgtt gattctttgg tttattgact tagacatttc ttttcagcaa ataatcaatg
    24721 aaaaatcatt tgtcttggaa tatacgatgg ctattcttac cacactggcc gcactacttg
    24781 tcctgccctt aacttgcgtg actacagcat atctctttac aaaaaagaaa gaacatctca
    24841 agatggagta aaagttacaa cttaagtcct atgacatttt tactgaatca gtgcataata
    24901 tggatgtaag gaaaagcttg caaacaattt tgcttaagga ggcaatttcc atgaaagaaa
    24961 gagaacgtat tttagactta gtgaaaaaag gaatcctttc gactgaagaa ggcttagatt
    25021 tattagaaag catggcaact gaaaaagacg aaaaacaaat caaaaaagaa gcagacaagg
    25081 taactgctag tcataaagaa aaagatcagg caagtcaatt gatcgatgaa cttgaaaacg
    25141 gtgaagaaaa aatttctgag ccagttgatc caaaagaaaa agaaagacaa gatcaagaaa
    25201 acttagaaaa gatcttagat gaactagcaa ctgaagcgaa taaaacttcg gcgcgtctag
    25261 acgaagtaaa tgctcaattc gctgacaata aggcagcacg taatgagaaa caggaagcat
    25321 tgatgcagct gaatacaaaa gaagaattag gcgagttgac agaagaagaa ttagcacagc
    25381 gtcaaacgtt agaagcagaa atcaaagagt tagaagcaac cggcgataca ctattagaag
    25441 aacaattgaa gctggaagca gaattaaaag atatccgcaa gaatcaatgg tctgagaaaa
    25501 aagaaacatt tacggataag tttgaattgc cagatgattg gaaagatcaa gcgacagata
    25561 cgctgaatca agtaggcgaa aaaatgtcag aagctggatc acagttaggc aaattcttga
    25621 agaaaacatt ccaaactgtt tcagaaacag taaatgataa catggaatgg aaagatgtca
    25681 gcttacgcgt ccctggaatt gcgacaacga aatttgaaca cgaattttat tatgaagctc
    25741 ctgcagcaag tatccttgat atcaaggcag caaacggaaa cgtgacgtta aaaacgtggg
    25801 acagtgatga tgtgaaagtg gaagccaaga tcaaacttta cggaaaaatg ggtgctgaac
    25861 catttgaagc attctctgaa cggagccaga tcgaggtcaa tgaagatcat atctccttcc
    25921 aaattccaaa taagcgtgtg cgtgcagacc ttgttttcta cttgcctaaa cgagtatacg
    25981 accatgcagc tatcaaactt ctaaatggca acatcatgat tgagacattg gaagctaaag
    26041 atatttatac aaaatctacg aatgggaaca tcatcgtgaa ccaattaact gcaacgatgc
    26101 tggaagtaga aggtgtaaac ggcaatatcg atattcgaaa cgggaacatc ttagattcta
    26161 ttatagaaac agtaaacggt acagtgacat ttggtgccac accagaaaac ctttctgtgt
    26221 ctttagtaaa tggtgatgta cgtcttacga ttaaagaaga taatttgaaa aaagtagaag
    26281 caagttcagt gaacgggaat gtcaaagttg ctttaccaga tagcatcggt ttggaaggac
    26341 acgctaaaac aagtcttgga agcattaaca gccgtctgtc caattacgaa gtagttcgtg
    26401 agaaaaaaga acggacaaac caaatgttgc aattccgtcg tgtttcagat gatgagatcg
    26461 cacaagtgca gctttcaacg acaacaggaa gtatctattt aaaagataca gataaataaa
    26521 gaggttgtga aagagatgtt cagccttgag caataagaag cgatttttta aaatagcttc
    26581 ccctatttta aaaaaatgtg ccttattgcc gataggctat ctcttgaaca ccgattaaga
    26641 agaaggttgt cacaacgatt ttcttcattt aaaatagtag aacagaggtg agacatgatg
    26701 aaaaaaaagt tgacaaaatc caataaaaat gttgttttga caggtacttt agcagggatt
    26761 ggggaatata tcgggattga tcccactgtt atccgtgtgc tttatgtatt cttaagctta
    26821 gttgcttttg gaagtccaat cattttatac attttattag ctttgattat cccatctgcg
    26881 cgttctgcac gaacaaacta tggacataac aatgcatatt atgaaaaaaa caattatcgt
    26941 gaacaaacaa aaaaacgcaa agaagcggaa aaaatcgatg atgacgaatg gagtgacttt
    27001 taatgtcgta ctttcagcgt attttggtta atacgttaac attcatatcg ttggctgttc
    27061 tttttcctgc gatggtccat gtgagcagta tttggacggc cgtccttgcc agcttcgtat
    27121 tatctgtgtt gaacatgttt gtcaaaccga ttttgacgat cctgtccctg ccctttacat
    27181 tagtcacatt gggattgttt acgtttgtta tcaatgcagc gattcttaaa atgacttcct
    27241 tttttgtagg agcagaacat tttagttttt cttcatttgg tgcagcgtta ttcgtagcta
    27301 tcttgatgtc acttgtcaat gtaattgtta gtgaacataa cagcagaaaa taaaaaaagc
    27361 tgtgccgaga tctcggcgca gcttttttgt ttagaataaa gaatgcgttt tctattacat
    27421 tggtaattaa ataaaaacgt tttttttaat tggtagtact tttggctaga caatgataaa
    27481 atgatagaga acactctaat agaaatgagg tagcatgaat gtcagaagtt gtaaaagtta
    27541 gccaattagt aaaaaaatta tctttggaaa tcgtcacagg cgacgagaaa agtttagacc
    27601 gcgtcatcgt gactggggac atctcacgcc caggactaga gttgaccggt tattttaatt
    27661 attattccca taatcgttta cagctatttg gcagtaaaga aatcagtttt gcggaacgga
    27721 tgatcccaga agagcgtttg atcattatga gaagattatg tagtgaagat atgcccgcat
    27781 ttattatttc aagaggattg ccggctccgg aagaaatgat ccaagcagcc aatgaaaatg
    27841 ggatagctgt gctgcgttcg cctatctcaa cgtcccggct gctaggtgaa ttatctagtt
    27901 atttagatgg acgcttagcc cctcgaacaa gtgtccacgg tgtgttagtc gatgtctacg
    27961 gattaggtgt actaattcaa ggggacagtg ggatagggaa aagtgaaacg gcgttggaac
    28021 tgattaagcg cggacatcga ttgatcgcag acgatcgagt agatgtctat cagcaagatg
    28081 aactgacggt aatcggagaa cctcctaaaa tcctggaaca tctgattgag attcgaggga
    28141 tcggtatcat agatgtaatg aatcttttcg gtgcgagtgc ggtacgaggc tctatgcaag
    28201 tccagttagc tgtttatcta gaagcatggg cgaaagataa gaaatacgac cgtctgggct
    28261 ctgaagacac gaccgttgaa atcgcggaag tgggcattcc acaaataaaa atccctgtca
    28321 aaacaggacg aaacgttgca atcatcattg aagtagccgc aatgaatttc cgcgcacgta
    28381 caatgggctt tgacgcaacg aaaacatttg aagaaagatt aagtcgcttg attgaagaaa
    28441 actcaggcaa tgactagcaa ggagtgacaa tatgttagca caaatcaatc gagttgcttt
    28501 tgagcttgta ggaattccta tctactggta tgcattgatt atcgtatcag gaatcattat
    28561 tgccatgtgg ctcagcagca gagaagctgt tcgggtagga ttaaaagcag atgacgtgac
    28621 agattttatg ttgtggggat tgccgatttc cattattggt gcccgcttgt attatgtttt
    28681 attcgatctt ccccaatata tagctgatcc aatccagata ttcaatatcc gttcaggagg
    28741 attagcgatt tacgggggat tgattgcagg tgcaatcaca ctgtactttt ttaccaaata
    28801 tcattttatt tctttgtgga cctttttaga tatcgctgca ccaagcgtat tattggcgca
    28861 ggcaatcgga cgatggggaa atttcatgaa ccacgaagcg tatggtccag acacgacacg
    28921 aagtttctta gaaagtctgc atttaccgca attcatcatt gataacatgt atatcgaagg
    28981 aatctatcga caaccaacct ttctttatga atcactttgg agtttgattg gatttattct
    29041 gcttttggtt ttgcggaaga aaccacatct tttgaaaaaa ggagaagtcc ttttatgtta
    29101 tctaatgtgg tatagtttcg gacgtttctt tatcgaaggg ttacgcacag acagccttta
    29161 ctttttaggt gtgatccgca tttctcaatt attgtctgct gttttatttg tcggcacatt
    29221 gattctgttt atttggcgta gaaaaaaagg aagtttaccg gattatgacc gctcattagg
    29281 aaaaaatcaa caaattattt gattttcaga gcgttaaaaa tgaaaaaaat ttcaggagtg
    29341 gtaaaatgaa ttatagagta gcggttttag gaccaggctc ttgggggact gctttagcac
    29401 aagtcctcgc tgaaaacgga catgacgttc ggatttgggg acatcgtgct tcacaagtag
    29461 aagaaatcaa cacccaacac accaatcaac gttatttacc agatcatcat ctacccgatt
    29521 ccatccgagc attcaccgat atggaagatg cagtaaaaga tgcagatgcg gtactattcg
    29581 ttatcccgac gaaagcgata cgttctgttg caaaagaact agttgccaaa ttgaatacga
    29641 agccagtgat catccacgca agtaagggat tagaacaggg aacgcataaa agaatctctg
    29701 aaatcttgtt agaggaaatc ccgacagaaa aaagaaaagc tgttgctgtt ctttctgggc
    29761 caagccatgc ggaagaagta gcaaagcatg acattacgac gatcacagcg gcaagcacaa
    29821 atgtaaaagc agcaaaatat gtacaagagc tgttcatgaa ccattatttt cgaatctata
    29881 ccaaccctga tgtgattggt gtagagatgg gagcagcttt gaaaaatatt attgccattg
    29941 gagctggtgc tattcacggc ttagggtttg gcgataatgc aaaagcggca atcatgacgc
    30001 gcggattagc tgagatcagc cgtttaggtg tagcaatggg tgccaacccg ctgaccttta
    30061 ttggcttaag cggagtaggt gatctagtcg ttacttgcac aagtgttcat tccagaaact
    30121 ggcgcgcagg caacttgtta ggacaaggtc agcctctaga agaggtgcta gccaatatgg
    30181 gaatggttgt tgaaggtgtg aatactactc aagcagcgat ggaactagcc caaatgatgg
    30241 aaatcgaaat gcccatcact caaacaatct atgaagtgct gtatgaacaa aaagaaatca
    30301 aaacagccgc aaaagaaatc atgttacgtg atgggaaaaa agaaaatgaa ttctcattaa
    30361 caattttttg aaggagcgaa tgtatatgaa agtaaaaaaa gcagttattc cagcagcagg
    30421 attaggaaca agatttttac cagcaacaaa ggcgatggca aaggaaatgt tgccaatcgt
    30481 ggacaaacca acgatccaat tcatcgttga agaagcgttg aaatcaggaa tcgaagacat
    30541 cttgatcgtt acaggaaaag ctaaacgacc aatcgaagat cactttgatt caaatatcga
    30601 attagaaaca aacctaagtg aaaaaggaaa aaccgaatta ttgaaattag tagaagaaac
    30661 gacagatgtc aatttgcatt tcatccgtca atctcatcca aaggggttag gacacgccgt
    30721 tcttcaagca agagccttta ttggaaatga accgtttgtc gttatgctag gcgatgatat
    30781 catggaagac gaagtaccat tgacacagca attgatcgat gactatgagc gtacacacgc
    30841 ttctacgatt gccgttatgc aagttcctca cgatgaaact tcaaaatacg gaatcatcaa
    30901 cccaggggaa gtactggaag atgggttata caatgtcaaa aactttgttg aaaaaccaga
    30961 tccaagcaaa gcaccaagtg atttagctat tattgggcgt tacttattga ctccagaaat
    31021 ctttgatgta ttatcagaac aacaaccagg cgctggaaat gagatccaac taactgacgc
    31081 aatcgatacg ttgaataaaa cacaacgcgt atttgcccgt aaattcacag gtaaacgtta
    31141 tgacgttggt gacaaatttg gttttatgaa aacaagtatt gaatatggtc tgacacatcc
    31201 agaagtaggg gcacctttgc gtgaatacat catgtcttta ggtaaaacat tagaagcaga
    31261 aggaagcaag atcaatcata atccaaaagg aataacagaa atcaaagaag aaaagaaaaa
    31321 ataatctttt tttctaacga gcttcagctg ctaattgaat aaaaagtagt tgaagctcgt
    31381 ttttatttgt ttaaaaaaca cgaaaatgct atccttatag aaaagagaga caagaaggga
    31441 gtttgatcat gtcaggagga gaaattgcag gattgatcgc agcaatcgcc tttgcgatac
    31501 ttgtcgtttt catcgttcgc gtattgttgc aagtttccaa aactttagaa aaagtagacc
    31561 agactgtcgc agaagcaaat acgacaattg agatcgtcac acaagacgta gatcttttat
    31621 ctagacaagt agaaggcttg ctagtcaaaa gtaatgagct gctcttggat atcaatggaa
    31681 aagtcgcaac gatcgatcca ttgttcacag cagttgctga tttaagtgaa agtgtatcgg
    31741 aacttaatca ttctagtaaa aacatcgtga cgaaagtcgg cggaatcgga aaaacgacag
    31801 ctaaagcgac tgttgccgga aaagtcggtg gaacagcgat gaaattttta aaaacaaaaa
    31861 acatactgaa acgacaggag gaaaataaga atggctaaaa aaggtggatt tttattaggt
    31921 gcagtagttg gaggtacagc cgcagcaatc gccgcattat tgttagcacc caaatctggt
    31981 aaagaacttc gggatgaatt ggctgctcaa gcagatgatt tgatggacat ggcttcagat
    32041 tacacagata tggcaaaaga aaaatcatct gagttaacag gtattgcaaa agaaaaagcg
    32101 caagatcttt ctcaacaagc aggggatcta gcaggcaatg ttaagcagaa ggtaagtgaa
    32161 tcagcagagg aagcaggaag cgaatcagat gatatcttgt taaacctcaa agaacaatca
    32221 gctgatttat ctaatcgttt caaaaaaaca gcagaagatc tgaaaaagcc agcagacgaa
    32281 ttaggtagta ttgctaaaga tacttcagaa gatatcatga tcgatgtaaa agatgctgca
    32341 gataaagcag gagagacggt caatgaagga atgtcagaag caaaaccagt tgcagatgac
    32401 acaaaagaaa tgttgaaaat ggcaaaagaa gatgcaaaag aagatgcaaa agaagctaaa
    32461 aaagaaatga aagatacaat gacagattcg actaccaatc actaagatta ctcatttcta
    32521 gaataaaaaa ggtgagagcg gaccagctct cacctttttt attttcatta ctctaaaaca
    32581 agtaattctt ttggtgtagt cgtgaatggc acacaaccat cacttgtcac atacacacaa
    32641 tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag agagaagcac
    32701 atcccttctt caatcactaa gtcgtttcct tctacaagag aagggaattc gtggacggtt
    32761 gttccgattc cgtgacctaa acggtgattg aaatattcgc cgtagccata gctgttgatc
    32821 acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctgggcgaac tgcctcttgt
    32881 gcggcaagct gtgcttcaag agtgatgtta tagatttttt cttggaattc agttggtttt
    32941 tgataagcaa ctgtacgagt agcatcactg caatagccat tccaaataac gccaagatcg
    33001 aataacacta gttcatttgg tgaaaccttc gtgcttcctg gtgtaccatg agggcttgca
    33061 gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc ttgttttttt
    33121 aattggtatt cgatttctgc gataattgct tgttcagtta cgccttcttt tactgttttg
    33181 aagccaattt caaaggcaac atcggcccat ttaccagctt cgatcagttt gtctttttct
    33241 tctaccgttt tgatcagttg cagacgctgg ataatagaag ttagatcggt gtaagaatct
    33301 gtacctgtca agatatcaga gatagcatcg aagcgatcga cagtcagagc attcttttca
    33361 atacctacat taccaggttg cccatagcgt gcgcgaagct cttttccgat gatctcccaa
    33421 ggattttcgc tgtctaagta gccacgtaca tcgtacgccc agctgctgtt ttgagcatct
    33481 tcaacttcca gagcaggagt gaaaaggaaa ggatcttgtt ccagagggat aaaaagagct
    33541 agtacacgct cgtgaggatc actttcatat ccggaaaagt aagcgatgtg gccagggtcg
    33601 ctgatataag ccaattctaa ttggtgttgt gccatccatt gacgtagttc attgattttt
    33661 acgttattca tttggcaacc tactttctaa attaattttg tattcaataa ttgtaccact
    33721 gattgattaa aagaccaacg ttcttaaaac tgaaaaaagt aaaaattttc atgaaaacag
    33781 agataaaacg gttgcaattg ttgagattat ttgatatcct attttagaaa tgtaagacgt
    33841 attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc aaacaatcac
    33901 tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac gtgtcgtaaa
    33961 tggcaatcca aacgtcaaac cagcaacaag aaaaaaagtc ttggaagtta tcgatcgctt
    34021 ggattatcgt ccaaatgcag tcgcacgtgg cttggcaagt aagaaaacaa caacagttgg
    34081 ggtaatcatt cctgatgtca gcaatatgtt ctttgcctct ttagcacgtg gtatcgacga
    34141 tgtggcaaca atgtacaaat acaatatcat cttagcaaac tcagatggca acgatcaaaa
    34201 agaagtcaat gtattgaata atttattggc taagcaagtg gacggcgtga tctttatggg
    34261 tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaacgc ctgttgtact
    34321 agctggttcc attgatccag atgaacaagt aggtagcgta aacattgatt atgtatcagc
    34381 aacaaaagat gcagtaaata aattagcaaa aagcggcaat aaaaaaatcg cttttgtcag
    34441 cggtgcgctg attgatccta tcaatggtca aaaccgtcta aaaggctata aagaagcatt
    34501 aagcgaaaac aatctaccat atagcgaagg attgattttt gaggcacaat acaactttaa
    34561 agatggtctg tctctagttg accggatcca taatagcggt gcaacagctg tctatgtttc
    34621 agacgatgaa ttagccattg gtatcttgga tggtttatta gaccgcggcg taaaagtacc
    34681 agaagaattc gaaatcatca caagcaacaa ttctttactg acagaagtag ctcgtcctcg
    34741 tttgacaagt atcacacaac cattatacga tttaggagcg gtatccatgc gcctattgac
    34801 gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt atggaatcga
    34861 agaaaaagga tcaactaaat aataaaagat caatcattta aaggttgttc ccaaagtggt
    34921 caacttctgg aacaaccttt atacgagaaa aggggttatg acaaaatttt gtcataaccc
    34981 ctttttttgt gactttaaaa aaaagagaga atggcggaaa caaaccttac gtccatcgta
    35041 tacgacacaa tctgtgcgta gatgctacat tttgttcact actatcaaaa ttactttaaa
    35101 atgaaaacgt gataaaaaag aaagtagaaa agtagaaaag taactctaaa aaatccaaaa
    35161 aaatccaggc tcgattgcgt acttatctta aaggagaaac aatccatcat acataacccg
    35221 aaaggagact tctatttgcc taagaaaaga cgaaagacca tagtcatcat tttgaccatt
    35281 cttactatag gaatcggagg aaaattttac atggacaaaa gagaagcgca aaaacagcaa
    35341 gatttactcg cagtagaaaa acagtccgtc aaagttctga aaaatacctt tgcggatatc
    35401 aaagaagtga aaatcgaaca atttgagaaa aacgacatga caggaatcta tgaagcattg
    35461 ataacgatga ccaatacaga aggaaaagcc gtttattttg attatagctt tacaaaagaa
    35521 agcaatgaga tagatggcta tggtgttgaa aatgaagaag tacaaaaaga agggattacg
    35581 gttgatgaga ttatagttat ctatacagat gggagtgagg aaaaagtatg acaattactg
    35641 ataagcagta taaaggaata agtaataaag tatattggct agatccaaaa catcctcaat
    35701 atagtaaaag ttacaaagaa ggcgccatta aaaactttgg agaaacaaaa ttccaaatcc
    35761 taaaaatcaa aaactccttc gacggcatgc aggcgatggc tgtagcgcca atcaagaacg
    35821 gtaaagtgga tatttctcaa gtagtgattg cttttgcagg aacgaactta agcgattgga
    35881 aagatttgga aacggatgca agaagtgtta tgaatctcta tgggaataga ccaggagcaa
    35941 gtttttctga aaaagcaaca acaaacaaga ttttatccgt tagtggattg aaaacagcgg
    36001 ggcaaatcaa ttcagcgatt gcatttgcta atgaagtaaa aaaggaatat ggagaagctg
    36061 ctatttctac taccggccat tcattaggtg gattcttagc attatatgta gcagcagaga
    36121 aacaatggaa aaatgtcggc ttcaacgggc cggacccgta tgatctctta tcaccagaag
    36181 caaaaaaatg ggtggaagaa aatcccggta tgctcaccaa ttaccgaaat cacgccgatt
    36241 ttatcggtaa ttttggtggt aatggcacag gtgctgaagt tcggatcagt atggaaacag
    36301 catacttgaa tctgcttgct tcccacagtt taacctcttg gaagtttgat gaaaatggaa
    36361 agctgattat tcctgaaaat gaaaacaatg ttaaagctcg aaaacaacag aaagaaaacc
    36421 aagcgaccca gttgttttcc tatcagttgc aagaactgaa caacatacgt aaaaaactac
    36481 gattaagtgg cggccggctg acagttaatg aacaaattta cttagatgac acacaagcta
    36541 ggttagtggt ggaaaagtta tcggtaaaaa tgaaaagcgc tatggaagaa gttatccgca
    36601 tcaaccaaag aggaattttt gagctagaga aaaaatggca agaaggcttg cggcaagcac
    36661 gtgttgtcgc cccttcgttg actgaacagg aaatcataga agcattagct agtgtgggcg
    36721 ccacaaaaga gaacttagtc gattctgcga aagaagtcta tctgctacga atcaataagg
    36781 caagacgtat gggggaggcc tttgatacat tggcgaagga gatccaagac aaaatcaatg
    36841 aaatcgtggc acgagatcga gaattagcac agcaattcaa ttaaaggaga agtagatgaa
    36901 actagaagaa ttggaaagag attatcgaag tgaacgaaga caattagaag ctcaagaaga
    36961 tgaattgatc cactttaaga atcaaggatt acgattgatg gagcaagagg agcagcgact
    37021 gcatcatgta ttaaagaact tagctttaga taatgagcct ctgtttcaag caagacgaag
    37081 ttattcgaat tatcaggagc attatcaaca agccctacaa gtagaacgac gaaaactcta
    37141 tcagcagcaa gacgaactag aacgttccta tcgtcaaaaa atcagacaaa taactatgga
    37201 gaaatcatct tatgaatgaa tttttaatgc aattactaaa agaattagag aaaaatctac
    37261 atcgatcaaa tgtacgttca gttggtatgg ggatatctca gcaaacttgg gcagaaaaac
    37321 gattgacaga tcaaaaccac caaatgactg cagaagaagc tactcaagaa ttttctaacc
    37381 gacatgcaca cttaaaagaa tcagtaaaag ggcaatttgc agaaaaacta accgaattat
    37441 tccaaagaca ag
//
LOCUS       DAZUMM010000019        37050 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000019 DAZUMM010000000
VERSION     DAZUMM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.019"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..788)
                     /locus_tag="ACG8E6_000907"
     CDS             complement(<1..788)
                     /locus_tag="ACG8E6_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFS5061346.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNII"
     gene            complement(761..1441)
                     /locus_tag="ACG8E6_000908"
     CDS             complement(761..1441)
                     /locus_tag="ACG8E6_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS5061347.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1464..2402)
                     /locus_tag="ACG8E6_000909"
     CDS             complement(1464..2402)
                     /locus_tag="ACG8E6_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS5061348.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2647..3498)
                     /gene="ispE"
                     /locus_tag="ACG8E6_000910"
     CDS             complement(2647..3498)
                     /gene="ispE"
                     /locus_tag="ACG8E6_000910"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFS5061349.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3652..6225)
                     /gene="mprF"
                     /locus_tag="ACG8E6_000911"
     CDS             complement(3652..6225)
                     /gene="mprF"
                     /locus_tag="ACG8E6_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS5061350.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6366..7172
                     /gene="proC"
                     /locus_tag="ACG8E6_000912"
     CDS             6366..7172
                     /gene="proC"
                     /locus_tag="ACG8E6_000912"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFS5061351.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7251..8807)
                     /gene="rny"
                     /locus_tag="ACG8E6_000913"
     CDS             complement(7251..8807)
                     /gene="rny"
                     /locus_tag="ACG8E6_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFS5061352.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9218..10267)
                     /gene="recA"
                     /locus_tag="ACG8E6_000914"
     CDS             complement(9218..10267)
                     /gene="recA"
                     /locus_tag="ACG8E6_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFS5061353.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10376..11617)
                     /locus_tag="ACG8E6_000915"
     CDS             complement(10376..11617)
                     /locus_tag="ACG8E6_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFS5061354.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11857..12435)
                     /gene="pgsA"
                     /locus_tag="ACG8E6_000916"
     CDS             complement(11857..12435)
                     /gene="pgsA"
                     /locus_tag="ACG8E6_000916"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFS5061355.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12644..13567)
                     /locus_tag="ACG8E6_000917"
     CDS             complement(12644..13567)
                     /locus_tag="ACG8E6_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFS5061356.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14306..15592)
                     /locus_tag="ACG8E6_000918"
     CDS             complement(14306..15592)
                     /locus_tag="ACG8E6_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS5061357.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15585..16853)
                     /locus_tag="ACG8E6_000919"
     CDS             complement(15585..16853)
                     /locus_tag="ACG8E6_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS5061358.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17105..17566
                     /gene="mscL"
                     /locus_tag="ACG8E6_000920"
     CDS             17105..17566
                     /gene="mscL"
                     /locus_tag="ACG8E6_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFS5061359.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17625..18071)
                     /locus_tag="ACG8E6_000921"
     CDS             complement(17625..18071)
                     /locus_tag="ACG8E6_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5061360.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18164..18409)
                     /locus_tag="ACG8E6_000922"
     CDS             complement(18164..18409)
                     /locus_tag="ACG8E6_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFS5061361.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(18524..19102)
                     /locus_tag="ACG8E6_000923"
     CDS             complement(18524..19102)
                     /locus_tag="ACG8E6_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5061362.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(19179..19709)
                     /locus_tag="ACG8E6_000924"
     CDS             complement(19179..19709)
                     /locus_tag="ACG8E6_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFS5061363.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(19713..20573)
                     /gene="rlmB"
                     /locus_tag="ACG8E6_000925"
     CDS             complement(19713..20573)
                     /gene="rlmB"
                     /locus_tag="ACG8E6_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFS5061364.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(20566..20961)
                     /locus_tag="ACG8E6_000926"
     CDS             complement(20566..20961)
                     /locus_tag="ACG8E6_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFS5061365.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(20958..22370)
                     /gene="cysS"
                     /locus_tag="ACG8E6_000927"
     CDS             complement(20958..22370)
                     /gene="cysS"
                     /locus_tag="ACG8E6_000927"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFS5061366.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(22367..22903)
                     /gene="epsC"
                     /locus_tag="ACG8E6_000928"
     CDS             complement(22367..22903)
                     /gene="epsC"
                     /locus_tag="ACG8E6_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFS5061367.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(22989..23228)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23225..24685)
                     /gene="gltX"
                     /locus_tag="ACG8E6_000929"
     CDS             complement(23225..24685)
                     /gene="gltX"
                     /locus_tag="ACG8E6_000929"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFS5061368.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(24855..26012)
                     /locus_tag="ACG8E6_000930"
     CDS             complement(24855..26012)
                     /locus_tag="ACG8E6_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFS5061369.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(26046..27416)
                     /gene="radA"
                     /locus_tag="ACG8E6_000931"
     CDS             complement(26046..27416)
                     /gene="radA"
                     /locus_tag="ACG8E6_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFS5061370.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(27479..28006)
                     /locus_tag="ACG8E6_000932"
     CDS             complement(27479..28006)
                     /locus_tag="ACG8E6_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFS5061371.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            28236..29042
                     /gene="proB"
                     /locus_tag="ACG8E6_000933"
     CDS             28236..29042
                     /gene="proB"
                     /locus_tag="ACG8E6_000933"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFS5061372.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            29039..30286
                     /locus_tag="ACG8E6_000934"
     CDS             29039..30286
                     /locus_tag="ACG8E6_000934"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFS5061373.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            30362..30706
                     /locus_tag="ACG8E6_000935"
     CDS             30362..30706
                     /locus_tag="ACG8E6_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061374.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(30823..31149)
                     /locus_tag="ACG8E6_000936"
     CDS             complement(30823..31149)
                     /locus_tag="ACG8E6_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061375.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(31522..32723)
                     /locus_tag="ACG8E6_000937"
                     /pseudo
     CDS             complement(31522..32723)
                     /locus_tag="ACG8E6_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            32840..33355
                     /locus_tag="ACG8E6_000938"
     CDS             32840..33355
                     /locus_tag="ACG8E6_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5061376.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(33554..34000)
                     /locus_tag="ACG8E6_000939"
     CDS             complement(33554..34000)
                     /locus_tag="ACG8E6_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS5061377.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(34047..35525)
                     /gene="msr(C)"
                     /locus_tag="ACG8E6_000940"
     CDS             complement(34047..35525)
                     /gene="msr(C)"
                     /locus_tag="ACG8E6_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS5061378.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(35879..36508)
                     /locus_tag="ACG8E6_000941"
     CDS             complement(35879..36508)
                     /locus_tag="ACG8E6_000941"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS5061379.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(36492..36956)
                     /locus_tag="ACG8E6_000942"
     CDS             complement(36492..36956)
                     /locus_tag="ACG8E6_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5061380.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
BASE COUNT        10480 a         8068 c         6574 g        11928 t
ORIGIN      
        1 ttgatgatat ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga
       61 ggtgtatcta gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa
      121 acactgatag caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga
      181 agaaccatga ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc
      241 acaccagtaa tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg
      301 gcggtatctt catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata
      361 ccgatggcaa cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc
      421 gaaccaaaca gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat
      481 actaacgcta gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt
      541 cggtagatcg ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc
      601 gtaagtgtag gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga
      661 gaaagcgtat cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg
      721 gcgattccgg caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc
      781 aacgccattg agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga
      841 tatcttcatg atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc
      901 ggagaagacg ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa
      961 gaacaaataa gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc
     1021 cgccagacaa ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag
     1081 cacgctgtac atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat
     1141 atcttcctga acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa
     1201 cttgttgagg aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct
     1261 ttatcattcc ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg
     1321 ctgctccatt ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat
     1381 cttctagtac aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca
     1441 ccttacaacc tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt
     1501 tccatgacag aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa
     1561 ggattcaaca cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa
     1621 gaggcatttt cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc
     1681 tcagctaagc gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt
     1741 tttaagtcgt attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg
     1801 tttgttgcat cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata
     1861 taagcatcgg cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct
     1921 tcagcaatcg ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg
     1981 tattcatgtg aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat
     2041 tctaaacggt cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa
     2101 tcctttaacg aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca
     2161 tcggaaattt ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca
     2221 gggatcaaaa gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc
     2281 ggataaaaag tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt
     2341 ccacatgctg caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc
     2401 atttatatgc tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat
     2461 gtatcaaaat aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat
     2521 attcttgatt aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa
     2581 taaaaaactg aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct
     2641 tatattttat tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt
     2701 atatactctc tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc
     2761 actcatcaat gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac
     2821 tggatgccgc tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg
     2881 ataatcttgc tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg
     2941 cgtatctact tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac
     3001 aacccagcac tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa
     3061 tgccgtactt ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc
     3121 cagttcttct aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc
     3181 acagtctgta cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat
     3241 cttgacaccg gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt
     3301 gttccgttca tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt
     3361 tatttcttca aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc
     3421 atgataccca tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc
     3481 tttttcaatc aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc
     3541 atagggaaaa ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca
     3601 acagctttca cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc
     3661 tgtgagcact tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag
     3721 tagctgcatg gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc
     3781 ccaatgatcg acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt
     3841 accataatta tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc
     3901 aacattagct aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac
     3961 aataaacaga ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg
     4021 caacaaatca tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac
     4081 tttttgatct ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg
     4141 tcccacagag aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac
     4201 agcttgatgt aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat
     4261 caaacgttga tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac
     4321 aatccctgct tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta
     4381 gctttctgat atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa
     4441 ctgattcgtt gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca
     4501 tttgtttcct ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata
     4561 aaaataactg taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc
     4621 caataaacgg tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc
     4681 taaatattga taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga
     4741 gaaccacaca tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa
     4801 aaagcgtccg ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct
     4861 agcatcaaag attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct
     4921 ggataaccac acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt
     4981 acgtgtgatt gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt
     5041 tttgactttt actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt
     5101 cactgtctga tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat
     5161 tgcagacaga ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc
     5221 acttacgagc acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa
     5281 tcgattgatt ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag
     5341 aacataataa aacaaccgat aatataatag ccaaactact gctacatctt gccgaacagg
     5401 caattgactc attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat
     5461 cgtcagcatt cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat
     5521 atctattctc atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc
     5581 aaaggatgcc agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt
     5641 tctctttaga taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca
     5701 gatccaatag cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag
     5761 aaacgaccat acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa
     5821 aaccattttt ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac
     5881 accaccaaaa ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc
     5941 ttttctttcc atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata
     6001 caacaacatc ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg
     6061 gctttgttgt cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc
     6121 gacttgattt gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact
     6181 atgttccttt atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta
     6241 acttgtatac ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt
     6301 agacctgttc tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg
     6361 ataacatgaa agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt
     6421 tattaaaaaa aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa
     6481 atgcacaaaa atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg
     6541 catcacaatc agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg
     6601 aagaactaag agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat
     6661 taacagattt agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga
     6721 atgtcaatgt cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg
     6781 aggaacagct tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag
     6841 cagaatcaga tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca
     6901 tgctgatcga tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca
     6961 ctgagattgc tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa
     7021 atccatggga acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag
     7081 ttgccttaga agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg
     7141 agaaagatct tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca
     7201 aaaaaagaag agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca
     7261 taatcagttg cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt
     7321 tcttcttcga ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt
     7381 tcttctggct tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct
     7441 acaccggcga agctgttcga aatattttcc agacttctta aacgacggat gtagttttct
     7501 aatgattcac ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg
     7561 acagagataa cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact
     7621 actgggtttt ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca
     7681 atctcatggt caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg
     7741 atatcttcgc caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc
     7801 ttcaagacgt tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga
     7861 tctggatgaa gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata
     7921 cgttcatcca tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt
     7981 cgtccatctt gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg
     8041 aatccggaaa gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc
     8101 gtctcaagcg ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca
     8161 ttcggaaggg taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga
     8221 atcgctaatg ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca
     8281 gattctttta ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg
     8341 acatctcgtg cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct
     8401 tcaatcattt tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt
     8461 gcttcttctt tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc
     8521 agcgtttgtt cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct
     8581 ctcagctcac tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat
     8641 gcttcttttt ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat
     8701 gattgtgcac cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca
     8761 agaccgacaa ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc
     8821 atactatctt tttcattttt atcatctata cagcttctat gataaattat tctaaactcg
     8881 tcaatatgca tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt
     8941 gtaccccgta gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac
     9001 gatggttttt aaaataaatg acactagttt catataggta aacctttaaa ttgagaaact
     9061 tttctattaa attattcttt gtagatgaac agatattttt attttatgta cccactcgta
     9121 taattaaata actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc
     9181 tactcttcag accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt
     9241 cttcttgtcc tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc
     9301 taactagagc agaaacttct gccatcattt caggatgatt agccatatag attttagcgt
     9361 tttcccgtcc ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa
     9421 caatgtcttt ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat
     9481 acatgacatc tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga
     9541 tctttgtacg gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt
     9601 ccaaacggat cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcc
     9661 caaacatcac gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt
     9721 tgttgatcga accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga
     9781 catgtgagtc acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat
     9841 cgactactac gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt
     9901 gttctcctgt gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg
     9961 catattgcgg atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt
    10021 gtacttctgc aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata
    10081 cttcgatgat acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag
    10141 agccacttgg aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc
    10201 ctttaccata gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat
    10261 ctgccaatac gataatcctc cttatattta caaatcagag caacatttca ttatccttat
    10321 catatctttt ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt
    10381 ttcttaaaag ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag
    10441 cagcacgatc tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag
    10501 cgatccatac tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga
    10561 aagctacact gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac
    10621 attctttgct gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag
    10681 cttttgtttg gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa
    10741 tgtcgcctaa tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct
    10801 gcttgttttc tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc
    10861 cataaaaata ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat
    10921 tccctgcctg aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg
    10981 catatggtgc gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca
    11041 gtcgcgattc accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag
    11101 gaaaacgttc ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag
    11161 gcggaccggg aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa
    11221 gccctgtacg atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt
    11281 ttttggtcat ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg
    11341 tattttgtat gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt
    11401 ctgtcggtcc taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa
    11461 tcaactcttc taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac
    11521 ctagatcagc tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta
    11581 aaagttctgt tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt
    11641 atacgctgta tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc
    11701 ttgtcacagc tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt
    11761 cacaatatac ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt
    11821 agacggcagg ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc
    11881 gctgtttttg atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag
    11941 catgatttga tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa
    12001 gataatagca accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac
    12061 ttctccatgt tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat
    12121 gatcgctact acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat
    12181 aactagcatt ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg
    12241 cgcacgggca atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc
    12301 acctactagc tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa
    12361 aggtaaacct acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt
    12421 atttggtaaa ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag
    12481 tttccaccga tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa
    12541 attgatcttt ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt
    12601 tttataccta caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa
    12661 gagtaaggtt cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt
    12721 ctccattcgc tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg
    12781 ctgtttccgg taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat
    12841 aggcattgtc tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta
    12901 caggcttggt agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag
    12961 ctattgccat tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt
    13021 tggtctgcgt gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg
    13081 aagaagctgt gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca
    13141 cgacaacgac aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa
    13201 agcgacttgg atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag
    13261 gctgcggacg ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta
    13321 aatggtctcc atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa
    13381 atttaccagg caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa
    13441 tttttgtaat ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta
    13501 atttttctcc gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc
    13561 gaagcatctg ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc
    13621 acagctcctt ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca
    13681 atatccaata aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac
    13741 ggcaggctca cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc
    13801 aaaaaccggc aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa
    13861 acgctgattt cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa
    13921 gtccaaccaa gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat
    13981 gcagggcagc tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca
    14041 ataagccgaa atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt
    14101 gaagctgacc acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata
    14161 agttgattaa aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta
    14221 agaataccat aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta
    14281 agacttttcc tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct
    14341 cttcacttat aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga
    14401 tttcaggaag atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa
    14461 tatattcgat cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat
    14521 cgagatttgt ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt
    14581 tttctgctgc tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac
    14641 gatccaagct gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta
    14701 gatagtttcg agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat
    14761 agcgtaacag ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa
    14821 atttatctcg tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt
    14881 cagggaaata acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc
    14941 gaataagttt catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc
    15001 ttggctgata aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac
    15061 tttcaaccgt gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta
    15121 tgccaaaaaa catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga
    15181 ttcctttttc cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca
    15241 gagtcgtcag attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa
    15301 aacttgtgaa agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat
    15361 cttctttttc aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct
    15421 tttctttttc accataggga ataaattcgt tatcgatcga accataattc gtactgaaca
    15481 aaccatatgt cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca
    15541 gtacttcatg ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt
    15601 cctcctgcta aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct
    15661 ttgatcgtta cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc
    15721 agccagctgt ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga
    15781 tattggtttc tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt
    15841 cgcaaacttt ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg
    15901 ataccggttt ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa
    15961 gctagacttt ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat
    16021 aaaccgttaa agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga
    16081 taagctaggt tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata
    16141 ttcacttgag gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt
    16201 aattgcccaa ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg
    16261 atttgatcat ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg
    16321 tccaaatctt cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa
    16381 agttcttgca aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat
    16441 agacgcagat tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata
    16501 tttggataaa ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca
    16561 ttgacatatt caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt
    16621 tttctaactg ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt
    16681 gtctgatccg gataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc
    16741 gtgatgactt cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat
    16801 tgttttgttg gtaagacatg aagattcacg ccttctgcca attgatacac catatttata
    16861 tgctcctttt cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta
    16921 cttattcctc tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt
    16981 tacactttat agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta
    17041 tattgttttt ttatttttta tttttcgata tactaaagac aaataaaaga agggagggtc
    17101 attcatgatc aaagaattca aagagttcat catgcgtgga gatgtcttgg atcttgctgt
    17161 cggcgttgtt attggtagtg cgtttacaag catcgtaaac caagtggtgc aaggcttgat
    17221 cacaccacta ataggttggt ttgtcgcaat gatcacaggt acttctgatt tagaaggagc
    17281 actttcaatt cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg gtaacgttat
    17341 tagtgctatc attacatttc tgatcactgg ttttgtctta ttcttagttg tcaaagcggc
    17401 aaaccgtgcg aagaatttac gctccaaaga agagcaagtg gaagtggaag aaactcctac
    17461 tgcagaagat tatcttagtg acatacgcga tcttcttgcc aaacaagtgg cagaagacaa
    17521 tacacatcca gtagagcaaa caaatctttc cgatacttct aaataataaa gaatctaaaa
    17581 aaaagaaagc cgattttcag tgtcggcttt ctttttttag attcttaact tgctacttgt
    17641 tcctttttca tgtcgatatg tggaatatca tcttccaaat acacatctga gattgccttg
    17701 aatccaaacg actcataaaa agcttgtagg tattcttgtg cgccgatcac gattggtttt
    17761 tctggaaaat ttttctggat catgttaatc gtttcttgaa caatctttct tcctaaacca
    17821 tctttacggt tgtcctgatg aacaaggaca cgtccaaaat ggatactctc cgcttcttca
    17881 tatactcgcg tgtaggcaag gatcttccca tcagtatctt gaaaaaacgt atgccaagct
    17941 tgtccatcaa tgtcatcaac ttcttgataa ggacactgtt gttccacaac aaaaactgcg
    18001 attcgttctt tgacgatcgc gtaaaactct gctgttgaca attcttcaaa tttctttacc
    18061 tgatattcca ctaagcaaac gccccctgct tttttattct tagcataccg attcgcacac
    18121 aaaaaagcaa acgaacctta cgaatctcag gctcgtttgc tttttaaact gcttcagtta
    18181 aaaattcgat ctcaactgtc cgagtaagta catctgaata gctgtaagat actcgttcaa
    18241 aagaattttc ttcaggatcc aaatcaacaa cgaatactga aggataggtt tctgttaaaa
    18301 cgccgcggcg ttctgtttga cgctttcttc ctgtctgagc aaccaacatg attgggctgc
    18361 cgatacgacc ttctaaatct ttcttgattg aagctaaagt tgttggcata cagttcacct
    18421 ctactatcaa ctataccaca tatttttaaa attttcaagt ttaccataaa aaaaaaagaa
    18481 atgcaataaa aataaataag aagctcatta agatttttct aaattattcc tcattataaa
    18541 atttcaagtg tttttttatt ttgagtttta ctctcccgta agcgctcatt acttgcttgt
    18601 gatccattgc taaacattca gccatgtcta caaaatcaac gccttgcata taataatgaa
    18661 aaattttagc ttctattttt gaaaactgga tttcgcaatt ttcgattgct tcttttaata
    18721 aaagatgctg aatcgctaaa ggttcttgca atgtgatctc tgctaaaaag tctggtcctt
    18781 ctgtctctat tttttggtcg attgagactt catctttttg atcacgacgc ttcagcgcgt
    18841 tctgcttcct caacagagaa tagatttgat tgcttataat gcttttataa taacttccaa
    18901 agctaacacc tttgcttttt tcgtaagttc tcaatgcctg atataaagcg atccttgctt
    18961 cttgttccca atcctcagaa acataatttc ttaaataata ggtgtttttc aaacgtagta
    19021 aaagtggaaa atatttttga tacagggcag caaatgcttc ttcgtcacca ttacgtgcag
    19081 cgcgcaataa ctcctcagac ataaaataat tcctccataa attcgtttat ggaggaatct
    19141 tatcatcaat gattcatcga actgattaga acgatatgtt atttattttt tctcattaaa
    19201 tcgtccaatt tttgggacaa tttttttaac tgctcttcat tccatggaga attacgtcgg
    19261 aaatcaataa aatgcatatc tttattttct tgggctattg ttttttccgt tttttttatc
    19321 gtcttatata attcataagc agaagttcgt aatgctcctt gggaaaagat catccactgt
    19381 tctgctaaat cgcttgtcgc cactgtcact tgtgtcaaac ggtcattcag ctgaccagcg
    19441 attctctcga tataactgtc agccgtttcg tcttctttcg taaaaacaac atccaactgg
    19501 tatttcttat aattttgttg gatgcccgga actagttggg catcgaagac aacgatgact
    19561 tccattcctt catatttcgc ataatttgat aaacgatgta aaagagcttc tcttgcatcc
    19621 gccattttgt cctgcttttt aagctggaca agttccggcc acgaaccaat catgttgtag
    19681 ccgtctacaa tcaacagctg ttttttcatg gtctactccc cctgacgttg ggaaaaagcc
    19741 tgatacataa gcaaaccagc agcaacactg gcatttaagc tctggacgtg cccggtcatc
    19801 gggatcgtca gcaattcatc catttctttt ttcaatcctg gactcattcc tttgccttca
    19861 ttaccaatga ctaaagcaat tgatcctttg gcattccatt tttggaagtt tgtgccggtc
    19921 atatctgtgc caaataccca aaatcctgtt tctttgagct gttgcacagc ttgtaccaaa
    19981 ttcgtcacgc gtgcaacagg gacatgttcg actgccccag tggaagtctt tgttacgata
    20041 ggtgtgattc caactgctcg gtgttttggt atgatcacac catcgacgcc acttgcgtct
    20101 gctgtccgta aaatcgaccc aaaattgtgt ggatcttcga tgctatccaa aatcaaaaag
    20161 aaggggtctt cttttttctc ttttgttcgt tcaatcagtt ggtcaaggga taaatattca
    20221 taaggtgtaa tcgcaagaac gactccttga tgcacgccgt gatcgctcaa agtttctaat
    20281 ttttgtttag gaacccattt gactggcaca gcttgttcac gtgcgatttc tttcagttct
    20341 tctacttttt ctcctcgtga atcttcttga agaaagagtt tattccctcg cccttgttgt
    20401 agcgcttcta ctgcggcatg gtgcccaaag acgaaattat cttctaatgc ttcttcattt
    20461 tgatcagaaa ctgactgatt tcgtccagtt tgctttttat ttgaatatct attttgtttt
    20521 ttgtcttcaa atccacgttt cgattgatgt ttgtttgttc tttctttacc catttttttc
    20581 accaactttc tgaatacacc agcggatcaa ttcttccatc cgctcggttt gttttgtcaa
    20641 atgcagatag cccatgagcg cttcgaaccc tgttgcgacc cgatacgtcg taacatcagc
    20701 attttttgcg ctcgtatggc ttttagcatt tcttcctctg cgataataaa gttcttcttc
    20761 ttctgtcaaa agatgttctt ccagcatttg ctgaatcaaa aaggcttgtg cattcgcaga
    20821 aacataattc gttgccgcac gatgcagctt attgggtttt gtttctcctt gatcaatcag
    20881 gtggtcacga acatagattt cgtaaatcgc atctccaaca taagccaagg ctaaaccgtt
    20941 caattgtgtg taatctctca tgcttctctt ctccatcggg ttccttgcgg tgtatcttct
    21001 aataagatcc ctttttcttt caacaaatca cgaatttcgt cgcttcggac aaaatcacga
    21061 ttgcttcttg cttgatttcg ttcttcgatc aattgttcaa tctcttcatc taataactct
    21121 tctgctgtaa ataaaatacc gaagatcgaa agccattgcg agaataaagc ttctccttgt
    21181 gccaagacag cggcagaaac tttcggctgg tctagataag tgttcagcca cttagcaagc
    21241 tcatagacca ctgtgatccc atttgctgca ttaaaatcat cgtccatttc ttcgatgaat
    21301 cgctgttcta aattttctaa caaagcgatt ttatccgcat catctgccaa attctctgag
    21361 gcagtttctt ggcggaattt caaattgtca aacgtattct tcagtcgttg taaattggtg
    21421 gctgcttctt tcatcgtcgt ttcactgtag cggatcggac gacggtattg tgtggtcgcc
    21481 atgaagaaac gcaagacttg tggatcaacg tttttgatca tttcatgtac agtgatgaaa
    21541 ttccctagag atttactcat tttttcatcg tcttcgccaa tcgttacata gccattgtgc
    21601 atccagtaat tagcgaattt tttccctgtt ttcgcttcac tttgtgcgat ttcattttca
    21661 tgatgaggaa attctaaatc ttgcccgcca ccatggatat cgatcgtttc tcctaaatgt
    21721 tttgttgcca taacggaaca ttcaatatgc caaccgggac gtcctttgcc ccatggtgaa
    21781 tcccaagaaa tctcgccagg ttttgcttgt ttccataaag cgaaatctaa tgggtcttct
    21841 ttttgctgct gttcttcccc tgtacgctga cttgctccga cttccagttc atcgatggat
    21901 tggtcactta atttcccata attttcaaat ttacgggtac ggtaatacac atcacctgaa
    21961 gattcataag catagccttt ttcaaccaac acttgaataa atgctaaaat atctggcata
    22021 tgatccatga ctcttggatg cagtgtagca ggctcgacat tcaacgcttt cgtatcttct
    22081 tcaaatgctc ggatgaaacg atcagcaact tctggtgctg taatacctaa ttcgtttgct
    22141 gtacggatga ttttatcgtc tacatccgtg aaattcgata cgaaatcgac tttatagcct
    22201 cgatattcaa aatagcgtcg aattgtatcg aaagcaatgg tgctacgagc atttccgata
    22261 tggatataat tatacacagt cggtccgcag acatacatac cgacatgtcc cttttgtata
    22321 gggatgaatt cttctttttt tctggtcaat gtattgtaga tttttatcat tctgatctcc
    22381 tcactttttc tcctttgatt cgaacgactt ttgctggtac tcctaccgct gtagcgtcgt
    22441 ctggaacatc tttcaacaca acggcacctg caccgatttt ggcgtttttt ccgatcgtta
    22501 ccggtcctaa tatctgcgca ttcgcagaga gcatggctcc ttgtttaaca gttgggtgtc
    22561 ttttacccgt ttcttttcct gtgccgccta atgttacgcc atggaataaa atcacatcgt
    22621 cctcgatttc tgcggtttcg ccaatgacga ttcccattcc atggtcgatg aacacgccag
    22681 ttccaatcgt tgctcctgga tggatctcaa caccagttag gaaacgccag aattgagcat
    22741 tgatcttcgc caataaaaag agccggtgct gatataaaaa atgagagaaa cgatgccaaa
    22801 acaaggcatg aaaaccaggg tatgtcaaaa cgatctcagc tgtcgaacgt gcagccggat
    22861 cgtttctctt tgctgcatca atcgctcggg taaaccaact catgactttc cactcctttc
    22921 gtaagaattt tatgtaggaa atcatcttct tataaagaaa agaagcgcct tttaaataag
    22981 tttccttatc aaaagacgct ttccgcgtgg ttccactttt cttcataatc aatgattatc
    23041 tctgacagct aacgcctgtt atacgttctt ggctactttg tttcctataa aacagttttt
    23101 cctgttttct gtgtttcgcc aagaccactc ccagaggcat ttcggcaacc attcattgat
    23161 accttgcacc acccggtatc tctcttgtaa tgatcagctg cttacttttt tctgttcatc
    23221 gtgtttagtt cagtacttga tttaaatgtg ctaatgattt ttcacgacca agcaattcga
    23281 tcgtttcagg caattctgga ccatgcattt gtcctgatac agctacacgg attggcatga
    23341 aaagattttt tccttttacg ccagtttctt tttgaacagc tttgatggca gctttgacag
    23401 ttggagcatc tactacttcc atttgttcta gctgtgcttt gaatgcgtgg agaacagtag
    23461 gaactgtttc accagcaagt acttcttttg ctgcttcatt caatacagga tgttcattga
    23521 aaaatagcgt agacagatca acgatttctg cagcatagct catttgaggt tgatacaggc
    23581 taacgatctt cttcacccat tcgattgtct tttcatctgg gttttctgct acacgtccat
    23641 cagcgattag ataaggcaga cacatgtcag taaatgcatc tagatccatt gctttgatgt
    23701 aatggttgtt tacccattgc agttttttcg catcgaaagc tgcgggtgat ttacttaggc
    23761 gttttggatc gaacattttg atcaattctt ggtgttcgaa gatttcgtct tcgcccacag
    23821 gtgaccagcc caataatgca ataaagttga acattgcatc tggtagatag cctaattcgc
    23881 gatattgttc gataaattgc aagattgatt cgtcacgttt gcttagtttt ttacctgttt
    23941 cactgttgat gatcaatgtc atatgtccga aggttggtgg tgtccagcca aatgcttcat
    24001 agatcatcag ttgttttggt gtattggcaa tatggtcatc tccacgtaaa acgtgagtga
    24061 ttttcatcaa atgatcatcg accgcaacag caaaattata agttggcata ccatcgcgtt
    24121 tttgtatgac aaagtcgcca ccgatattat cggattcgaa cgaaatagcg cctttcacca
    24181 tatctgtaaa tgcgtattct gtattgcgag gcacacggaa acgtacaaca gattctagac
    24241 cttgtgcttc tttttctgct tgttcttcag gtgtcaagtt cgcacatgtt cctgcgtaat
    24301 gaggcatttc tcctcttgcg cgttgtgctt cccgttctgc ttctaattct tcttctgtgc
    24361 aatagcattt gtatgctcga ttgctagcaa gcagctgatc aatcagcggt tgatagattt
    24421 ctttacgttc agattgacgg taagggccgt attctccagg attttcagga gattcatccc
    24481 aattcattcc caaccaagca aggttttcca actggctttt ttcgccatct tcgatattac
    24541 gtttttggtc agtatcctca atacgaataa taaagtctcc atcattgtgg cgggcaaaca
    24601 aataattgaa taatgcagtt cttgcatttc caatatgcag gtgcccagtt ggacttggtg
    24661 catagcgtac gcgtactttc gtcattttct tcctcttctt tcaaactaga ctttgactaa
    24721 cagcaaaaat tccgtcagtt acggcttctc aaatatcatt tatttaaagc cactcagcac
    24781 aaattttacc ttttctaagg taaatagtaa agccaactgg ttgaatttcc ttaccagttg
    24841 gctgaatttc ttttttattt tgattgttta gtctttttct ctgtctcttt tttattgtcg
    24901 attcctcgtc ctgcatgtgc aggtttagca aagatcatgc gtccagcagc tgtttgaagg
    24961 gcacttgtta caacgacttc gatacgttca ttcatgtaat gctgtccatc ttctacgaca
    25021 accatcgtgc cgtcatcaag gtaggctact ccttgttggc gttctgtccc ggcttttact
    25081 actagaacat tcatcgtttc accagggatg acgactggtt ttacggcatt tgccaacgca
    25141 ttgatattaa gaacaggtac attttggaat tcagagactt tattcaagtt atagtcattg
    25201 gttacaatga caccatctaa aagcttagcc aatttgatca atttgctgtc tacttcactg
    25261 atatcttcaa agtctccatc gtacatttcg acagatatac cctcttcttt ttgcaaggca
    25321 tttaggatat ctaatccgcg acgaccacgc acacgtttca aactatcccc tgagtccgcg
    25381 atgtattgta gttcgtaaag aacaaaatta gggatcatca aggttccttc taagaatcca
    25441 gtctttgtaa tatcataaat ccgaccgtca atgatcacgc ttgtatccaa gattttatat
    25501 ttatggaaat tttctcctga ttttcgttct aacagttgac tctcaacttg actagctgtt
    25561 tcttgctcga tttttttgct tcgtgaacca aaaactttct tccattcttc gattcgtgtc
    25621 gttcccattc tcaatcccag atagcctaag atgatcatca gtaaaatcgg caatacacta
    25681 ttgacgaatg gaatttccat attgtaaaga ggggtcgaga tcacaacacc taaagtcaat
    25741 ccgatgatcg caccgatcgc accgaagaga agataagtca agctgacctc acttagtttt
    25801 tgttctactt gtttgataat agctgagata tgtttggcca acagcaaaga taataaaaag
    25861 aaaataagtg cgcctaacag gctgttggta aatgtattat tcagccatcc gtttgactgt
    25921 tgaccgattg cctgccaagc tgagggtaaa aatgaaatac ccaagcttgc gccgacaatg
    25981 atcatcagca aagtaatgac acgtttttgc atcgtttttc ctccgtttcg acaatcattt
    26041 attagttagc ggaacacacg cttcaacgtt tcagccaaag ttgctactcc aactatctcg
    26101 attccttttg gcgcttccca gttacctaag ttgtttttag gtaggtatac ttttgtgaag
    26161 ccgagttttt ggacttcttt gactcgttgt tcaatatgac tgacacgtct gatttctcca
    26221 gtcagtccaa tttcaccgat gaaacattcc gttggctgtg ttcctttttc tttatagctt
    26281 gaagcaatac tgacagcgat agctaaatca atcgctggtt catttaattt taccccacct
    26341 gcagctttta agtaagcatc ttgattctga agaagcaacc cagctctttt ttccagcact
    26401 gccatgatca gagatacccg attgaaatca agacctgttg ttgttcgttt ggcatttccg
    26461 aacatagtag gtgtgactaa tgcttggatc tctaccaaaa ttggacgtgt cccttccatt
    26521 gccacaacga tagcagagcc agttgcaccg tctaggcgtt cttctaagaa aatctgtgaa
    26581 ggattagcga cttcttccaa tccatgctca tgcatttcaa aaatcccaat ctcattcgtt
    26641 gagccaaatc ggttttttac tgctcgcaaa atccgaaaac tatgatgttg ttcaccttca
    26701 aaatataaca ccgtatccac catatgttct aacattcttg ggccggcgat cgagccttct
    26761 ttggtaacat gaccgacgat aaaaatggct atcccatttg ttttagcgat tttgagtagt
    26821 tctgcagtgg tttctcttac ttggctgaca cttcctgcca cacttgtgat gtctggctgc
    26881 gtcatcgttt ggatggaatc aatgataaca taatcagggg agatatgttc gatggcacga
    26941 ctgatttcgt tcatatctgt ttcagcgtat agataaaatt ctgtgttgat acttccgagg
    27001 cgttccgcgc gtaattttat ctgctctgcg ctttcttcgc cggatacata taagactttt
    27061 ccgcctatgg ctgctaattg ctgagaaact tgtaacagca aagtggattt cccgatacct
    27121 ggatcgccac caatcaagac catagaccct ggcaccacac ctccgcctaa cacacggttc
    27181 agttcttcta attttgtttt gatccgaggt tcttttttag ggacgacgtc tgcgattttt
    27241 gttggttttg ctttttctcc tgtcaaactt gtccgagttc tgcggtccgt tgtatcttgt
    27301 tcgatttctt cgaccatcgt attccatttt ccgcaatttg ggcagcgtcc caagaactta
    27361 ggcgacacat acccgcaatt ttggcaaaca aattgtactt ttgatttttt tgccattgta
    27421 ccgcctcctt ttatcctaat catgagacat taaatcaagc ttaagtatct ttaacgtttt
    27481 atttactgga agaaccaaat ccgccttgac gttgagcagt cgtttgatct tgatctgctt
    27541 ttaaaaacgg caaaaagatc ccttggccga tacgctcgcc ttttttgatc actacatcct
    27601 tgaaaccaaa gttggtgaat tggaacatga tatggccttc atttgcctca ttattgtaat
    27661 aatcactgtc aatcacacct actccgtttg tcaacactaa aaaccgcttc aacggattgc
    27721 ttgaacggtt agcaagctgc aaaaattcat cttctcccat gtaagctttg attccagtag
    27781 gcacgagggt tggtttcaat tgtttttcat tttctgatac atcttttttc aatccatctg
    27841 cgactgtttc caaccccttt ttccagcctg ctttccagat acttggaatc acgacctctt
    27901 ctgctgcttc aaaatcatag ccagcagcat gatgtgtagc gcgctgcggt agattgatat
    27961 tttgatcttt atatttagaa ataacttcaa atcctctttt tctcaccggt tttcctccct
    28021 aaataaacta ctcctattct aacatatccg aggctgtaaa agactttctt tacaaattct
    28081 tcaagaaatt ctgctatctt aaagtaaacc attaattcgt tctttttcca aacgactgca
    28141 agttgtacaa aaaaactatt ttttacatga ctttactcgt caaaacaagt agaatagaac
    28201 gaaattaatg agtggatatt tgattgaggt gtcacatgcg taaagaaatt aaaaatgcaa
    28261 aacgaattat tgtcaaagta ggaacaagta cattgattta tcccaatgga aatatcaatc
    28321 ttgcggcaat cgatcagtta gcttttgtcc tttctgatct tagaaatcgg ggaaaggaag
    28381 tgatcttagt ctcatcagga gctgtaggcg ttggtatgca taaattacat ttgtcagaac
    28441 gtccgacttt gattcctgaa cagcaagctg cggcggctgt tggtcaagcc gaactgatga
    28501 atatctataa tcaaagattc agtgtatata atcaacagac ggcacagatc ctcgtgacaa
    28561 gagacgtggt agattatcct gaaagtcgta aaaatgtgag gaatacattg gaacagcttt
    28621 tgattatgga cacgatccct atcgtgaacg aaaacgatac tgtagcagtg gatgaattag
    28681 atcatctgac aaaatttggc gataatgatc agctgtcagc catcatggca aaacttgtcg
    28741 cggcagacct tttgatcgtc ctttctgata tcgacgggtt ttattcagat aatccgacta
    28801 caaatcccga ggctcagatg tatcgctata tcgataaaat cgatgaaaag attctggcac
    28861 aagcaggtgg tgctggtagc acatttggaa caggcggtat gcagagtaaa ttaaaagccg
    28921 cttctaggat atttgaagca aacagcgcaa tgattttagc aaatggaaaa aatccggcaa
    28981 ttatttttga tattcttgca ggaaaagaag taggtacttt atttaaggag gacttgaaat
    29041 gactgacttg atgcaattag gacaacaggc aaaagatagc gcgcaacaac tagcacttat
    29101 gcctacaaac cgcaaaaatg agctacttgt tcaaatggca caaaccatca aaaagaatca
    29161 acaagcgatc attgaagcaa atcataaaga cttagaaaaa gcagtcgaaa acaatatcag
    29221 tgaaacgatg caagaccgct tgcagctgac acctgagcga atcaatgcca tggctacaga
    29281 gatcgaaaaa attgcttcgt tagacgatcc tattggaaaa gtagatgaga tgtggacgaa
    29341 caccgacgga ttgagaatcg gcaaaaagcg cgtgccacta ggcgtgaccg gcatcattta
    29401 cgaatctcgt ccaaatgtca cgacagatgc agctagtcta gcattcaaat cagggaatgc
    29461 cgttatcctt cgcggtggca aagaagcatt cttctcgaat caattattag tccaattatt
    29521 gcagcaagta ctggtcagtg ctggcgaatc tccttatgcc atccagtttg tcgatgatac
    29581 ttctcatgaa actgcaggac agttgatgcg attgacagaa tacttagatg tgctgattcc
    29641 tcgaggcgga gcaaaattaa tccaacgagt caaagaacag gcaacggtac ctatcattga
    29701 aacaggtaca ggaaataatc acgtatatat cgataaagaa gcacagttat ctatggcagt
    29761 gaacatcatc gtcaatgcaa aagcttcccg tccatctgtc tgtaacgcgg cagaaacatt
    29821 gctgatccat tctgagatcg cacctttttt cttgccagct attgaaaaag aactggtaga
    29881 acacggggtc tctttacgtg cggatgctcg tgcgttagaa tatctagaga cggctgcttt
    29941 agcagaagac acagattggg atactgaata tctagattat atccttgctg tcaaagtggt
    30001 cgattctttg gatgaagcaa ttgcacatat caatcggcat aatactaaac attcagaaac
    30061 gatcgtgaca gatagttatg cggctagtca acgtttctta aatgaagtgg acgcggcggc
    30121 tgtctatgtc aatgcctcca ctcgttttac agacggatcg gtatttggtt ttggtgcaga
    30181 gattggtatc tctactcaaa agttgcatgc ccgtggtcca atggggttaa atgaattaac
    30241 ttccacaaaa tatatcattt atggcgatgg acaaatccgc gaataatgtt tcgtgtgaaa
    30301 cattattccc gccttttgcc ttttttctgt aaaggttcta tccaactaga aaggtgtcaa
    30361 tatgtttgaa attttaatga ttttttttat ttaccttatt tctctgaata tcgctgcttt
    30421 tctgggagtc ggtattttat cccttttctt tcaattcaaa aaacgaagca tagggagtca
    30481 acgggaaaaa tggtcgcagt attttgacaa aatcgggcaa aaagggctgg ttacacgctt
    30541 gcacattagt tacatggttg cattatgctt attagcgaca atcaattact attctttttt
    30601 cgatcattca atcgcttata cgattacgct actgattgct gggatttttc acttatctta
    30661 taaatatcag ctgaataaaa accacttgaa tcggactttc cgctaaaaaa tatccgtacg
    30721 ataagtagag tgccttgaag atcggcaaaa aactgccgga ctttttagag gctgtgacaa
    30781 agattttgtc acagcctctt tttccatata aacggcgttc gcctaccagc tgcctggtaa
    30841 atctgtttcc tcttcgttaa gaagcgaaaa tccgtcaaat gtttcgccat cataataacg
    30901 aagcagctca tccatggaca agttcatcgt tttttctttt tcttcttccg ctgattcagc
    30961 cagtagctgt tcccgataca cctgatacat accagctatt ttttgctgca tcgcttttct
    31021 agtttcacct tccgcattga ttattggcaa ttctttgata tagccacgat aaatcaaccg
    31081 atcatctgaa aattcaatgg gtaattcaac gagtgtcaac ccgacttcct gaaaatattc
    31141 ttccagcatc tattatcccc ccattctttt tcttaaatga attttagctt acctactgaa
    31201 aattttccag tttcattatt ttgagaaaaa aagggaatgt gacaaaaatc gtgtcacatc
    31261 ccctttttcc gaataaatgg tgttcttctg ccaactcctc aatcataagt cacaatattc
    31321 aacaaacatg agaaactgtt ttttgaaatt gttccttgtg attcagagtt aaacgacagt
    31381 ctcacaacct cttggtaaac ggcgttcaaa agcttgcatc tgcggtaata agcccaacca
    31441 aacgaaaaat atgagaagct atttttgttt ggctgttctt atgacttgat gcaggacagc
    31501 tttttcacaa cctctgattt cttattcttc ttcatttgta gcaaaataat tacggtgata
    31561 aatgatacaa catacactga taagaacaat ggcacctgca aaataaaacg catagctttc
    31621 accaatatgt tcaccaatca cacctgcaat aaatggtgct aaagctccac cgaaaaagcg
    31681 gacaaaacta tagctggacg aagcaacgtt tcgttgcaga cctggtattt ccatagcaat
    31741 cgtcgtcaac agtgtattga taattccttg gaaaaatcct gctaacacaa tgctgatcgg
    31801 tacaaggact ggcatagaag ttccagctcc taataacagc aaacaaataa tgaataagac
    31861 aaatgctgct aaaattgtcc gataagtcgt aaacttccgc tcaagagccg gtgcaacgaa
    31921 aatcgaagca atagccagaa gcacacccca gccaaaaaat acaaaaccga cttctaattc
    31981 agaatagcca atcaataaaa atggcgcata cgcaagtaaa gtaaagaatc cgaaattata
    32041 taacaatgcg attagtccaa ctgaacgaag tttatgattg cttaacgctt tgacaccttc
    32101 ccaaaggcct acttttctct ttggtttctc agtttcaggt aaaagtaccg ctagggctat
    32161 aaaaccgcaa aacatcaaaa atgctacccc aaaaaatgga taacgccatg aaccactgcc
    32221 caagacccca ccgactagag ggccaacagc cattccgcag ccaagtgctg cttcatacat
    32281 catgattgct ttttctgtgt taccaactaa aatactgacg atcgttgcta aagcagtaga
    32341 gacaaataaa gcattcccga caccccagcc ggcacgtaac ccaatcagca ttccgatatt
    32401 cccagataat ccgccaagac cagcgaatac aacgatgata aacaatcctg tcatcaatgt
    32461 tttttcaaac cgatccgact agataagaaa cctgtaaata gcatcaccac accagtgact
    32521 aacatgtaac tagtaaataa cagggtcgtt tcagctggcg tagcgtttaa ctgtgcagca
    32581 atcgttttta agattgggtc tactagaccc actcccataa aagcgatcat acaggtagct
    32641 gctacaacta gcgctgaact tttccccttt gtttcttcat ttgtttgttc ttgtatatct
    32701 atttgattct ccaatatttc catccctttc tctctattag catcatacat gatttatttt
    32761 acatgtaaat atgcacatgt aaaacgtgga aactttactg tgatataata aagaaaataa
    32821 gacgagaggt aggattttta tgaatacttt agcctatata ttattatgcg tgcttttatc
    32881 caaaccacgt tctgggtatg aattgaagca gctgatcaca attttttggg aagcccatca
    32941 cagtcagatc tatacaacgc ttgccaagtt agaccagcag ggttatgtga ccgttttgga
    33001 caaagacgag cactctcaaa aaaagatcta ccacttgact gatgaaggac gtgctcttgt
    33061 agaggaatgg atcaaagagg aaactcccgc tcctacgcaa aaagatgaat ttctagcaaa
    33121 aatctatgct ttttcgacgc tcgataaaac aacagctact ggactgctat tcactagaga
    33181 acaacagctg aacaaaatca tgcaaaaaaa tcaaaaaaaa ttgaatgacc tctcgctaga
    33241 caagaaacaa gaatttggac gttatgttgt gatccagcgc agaattatgc tttgtcagca
    33301 ggaactccaa tggtgtcagc aagtaagaga acaaatgcag cagttttttg agtaagggat
    33361 aaacagaacc tttctagaaa tatctattga agagcaaatt atcttcaata aaagagcaat
    33421 acagttggaa aagaaaaagc gatccgaacg acaggccaaa acctatcgtt cggatcacta
    33481 tttttctaga actgcattca agagactatg acaaaaagca tgccaaggcc ttttttcaaa
    33541 taacagtgtt ccatcattca ttactttgaa ttgcttttag cacacttctt gaaagcgggc
    33601 caacgatgat caactgcaac ggtaaagcgg caatcacatt cattttccaa gcattcatat
    33661 aagcagagaa taattgatca ggtattcctt gttccatgat cacaccgaat aaagacatac
    33721 atgtcaccat tcctaaaacc atcaagcatg aaatagttaa gatcatttgg accttgcttt
    33781 ctggatttac tggtagttta aaggcaattt ttttcgccag cacaccgatg atgaagacat
    33841 ccaaaatgaa agccaccacg aatccaggca ctagaccaat caacaaactt gataaatgaa
    33901 gatcttgatg cagccataaa ttatacgcgc tcatccccaa gaccatcaaa aaacacatca
    33961 ttgttgtaaa aattattcct tcttttttat ttgtaggcaa tctgtaccac tcctttaaat
    34021 ttgtcacgaa aatcatcgta gcacatttaa aaatttctcg taagtacttt ttttattttc
    34081 tgccctttta tttcataaac ttcatcagcc gtacgctcga caaagtaaga atcatgagaa
    34141 gtaaacaaaa tcgttcccgg atatatttgc attagcttct ctaaagcttc gatcgttgcc
    34201 atatcaataa aattagtcgg ttcatctaac agcaacacat tacttggctt agtaaaaagc
    34261 aaagcaatcg tcagacgggt cgcttctccc ccacttaatg tacataacgg tttcgtcaaa
    34321 gtttcctgag caaaccctaa gtgattcagc aaactacgga caagcgattc tgaaaactct
    34381 gtttgtctca tgacaaaacg aatgatggat tcttcagatg tcatttgata acccttttgc
    34441 tgatatactt gaaagcttac tttcggagag agaaggattc cttctccttg tttgcggata
    34501 tgttctaata aggttgtctt acctgaacca ttttcaccga caatcgcgat ttttttacct
    34561 aaagaaaact gaaaatcacc atttaccagt aatgttctcc cacttctttc caattggaca
    34621 ttttgtccca tgatgggata gcggctgtgg agttcaagag attttggtac tggaaaacgg
    34681 atttgtttca tctgctgcgg cttggttgtt tctcctattt tttggagtct tctctctaat
    34741 gttttcgcat tcttttggat ggctttttgt acactgtctt tttgtttaga ggaagacaac
    34801 cgatccggtc tgatttgttg ctttttcttt ttactggaca ctttttccaa cttttccgct
    34861 tgttttttct tttcttggat cgatttttcc aatcgttttt tctctttcat gatcgactct
    34921 gcttctcgta gctgagtctt tttctccaat tccttttgag tgagataggc actataattc
    34981 cccgaaaact ctcggatttc tccgtctttt acttcccaga ttttcgatgc tagctcatct
    35041 agaaaatagc gatcatgact aacaaaaacc agcgtaccat aataataacg aagctcttca
    35101 atcagtcgat caatactttt tctatctaga tgggttgttg gctcgtctag aagcatccct
    35161 tgttcataag aagaaagaag acgtatcaac tcgatctttc gttcttcccc tccgctccat
    35221 ttccgctctg aagtttcttc cagtcttaac tggaaatgac tggctaattc caaatttagg
    35281 tcctttgctt cagcgagata ggttaattga ggcaggtagt tgaaggtgat ctctgtttgg
    35341 attttcccat gatcgggaaa aagttcaccg gctattagtt tgagcaaagt cgatttaccg
    35401 acaccatttt ctcctatgat ccctatacga tcttgctgat agacacgaag agaatcaata
    35461 gatagttcta gctggttgcc aaagctgact tgtagatttg ttatatttac tgctaaattt
    35521 tccatatgtt ttacactcct atttcatgta aaacaacaat tatcgatact aaaaaagagc
    35581 agaaaaatct gtttgcaaaa aaactgcatc acatcttttc ctactctgaa cgaaataact
    35641 atgactaaat caccaaatct gtcatagctt tcacctattc tactgtcgtt cgaaaaaatg
    35701 gacatactcc atccatgaaa ttgttgttta tacatgtctt ttgaatttat ttagttgtta
    35761 ttcaaaagtt cgaaacgtaa tttcatcgat gcagtcattt ttccgacctc cttttttatt
    35821 aggttaccac acacgattca aaaaaagcaa tagctactga atagtaactg aaaacctttt
    35881 attcgaacgt agtgatcttt tctggtgcta aacgaacaga ctcgtaacct tcttctaccg
    35941 cttttcctat tgataaaata ataactggaa cataacggtc tttatccaaa ccaaatgcct
    36001 ctgccaactg atctgcttcg aatccaccaa ttggatttgt ttcatagcca tgatcacgcg
    36061 caactaacat gaattgcata gccgctaagc tagaatcgac tttgactaca tcgttcattt
    36121 cttctcttga aaaactcttg taataaggaa tgatcgcagc taattgttga tctctgactt
    36181 cttttggcat cttccctgat tcgtaagctt ggttatagat ttcttcgcca tattcgtaac
    36241 attgcatatc gccaaaaatc atgaccattg cagaagaagt atcattttgt ctcgtattga
    36301 aacggatcaa aggacgcaag atttcttttg cttcctcgct ttcagcgacc acaaagcgcc
    36361 atggttgcat attgacagaa gaaggtgcag tcgtcgcttc tttgatcatt tccagcattt
    36421 cttcgtgtgg gattttgtat gtttcatcat atacgcgaac ggatcttcta ccagtcataa
    36481 ttgttgaaaa atcatttttt tgttccatta ttattaacct ctttcgtttc tgttgttata
    36541 ttgcaaagtt gggtattcaa tttatctaaa agtgttagaa attgtttcat ttctacatcg
    36601 ctgaaagccg gggatagcac ggaacctgta tctttatggt ccatttcaca atgctcgatt
    36661 ttcttctggg ctttttctgt tagcgctaca tacacttccc ggttgttctg aggatttctc
    36721 tgacgcgaaa catagccatt tgcttccaat accttcagat gacgagtaac tgctccttga
    36781 tcaatctgca acttttcttg caaactgctt tgtagcaatg gattttcttc tttcagtctc
    36841 atcatgatct cgtatctagt aagacttata ccgatatttt cttcaaagat ctggacgatt
    36901 ttttgatcgg ctaatttgat ttgatataaa aggtgactaa tctcttctaa agacataatt
    36961 tcctcctttc taataattga ctcgtcaacg attgacttgt caataataga ataaaaaaag
    37021 aaaaaagtca aagaatcagc tgtgtaataa
//
LOCUS       DAZUMM010000020        36569 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000020 DAZUMM010000000
VERSION     DAZUMM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.020"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8E6_000943"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8E6_000943"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFS5061381.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8E6_000944"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8E6_000944"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFS5061382.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8E6_000945"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8E6_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFS5061383.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8E6_000946"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8E6_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFS5061384.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8E6_000947"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8E6_000947"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFS5061385.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8E6_000948"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8E6_000948"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFS5061386.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACG8E6_000949"
     CDS             complement(8053..8523)
                     /locus_tag="ACG8E6_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFS5061387.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACG8E6_000950"
     CDS             complement(8569..8994)
                     /locus_tag="ACG8E6_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFS5061388.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACG8E6_000951"
     CDS             complement(9061..9330)
                     /locus_tag="ACG8E6_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFS5061389.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACG8E6_000952"
     CDS             complement(9341..10936)
                     /locus_tag="ACG8E6_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5061390.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8E6_000953"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8E6_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFS5061391.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8E6_000954"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8E6_000954"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFS5061392.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACG8E6_000955"
     CDS             15402..16376
                     /locus_tag="ACG8E6_000955"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS5061393.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACG8E6_000956"
     CDS             16546..17946
                     /locus_tag="ACG8E6_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFS5061394.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACG8E6_000957"
     CDS             complement(18024..18746)
                     /locus_tag="ACG8E6_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5061395.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACG8E6_000958"
     CDS             complement(18825..19331)
                     /locus_tag="ACG8E6_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS5061396.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACG8E6_000959"
     CDS             19454..21253
                     /locus_tag="ACG8E6_000959"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFS5061397.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ACG8E6_000960"
     CDS             complement(21321..22895)
                     /locus_tag="ACG8E6_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS5061398.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACG8E6_000961"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACG8E6_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFS5061399.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACG8E6_000962"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACG8E6_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFS5061400.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="ACG8E6_000963"
     CDS             complement(24672..25310)
                     /locus_tag="ACG8E6_000963"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFS5061401.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACG8E6_000964"
     CDS             complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACG8E6_000964"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS5061402.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACG8E6_000965"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACG8E6_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFS5061403.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACG8E6_000966"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACG8E6_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFS5061404.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="ACG8E6_000967"
     CDS             complement(29104..29844)
                     /locus_tag="ACG8E6_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFS5061405.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACG8E6_000968"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACG8E6_000968"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFS5061406.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACG8E6_000969"
     CDS             complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACG8E6_000969"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFS5061407.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACG8E6_000970"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACG8E6_000970"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS5061408.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACG8E6_000971"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACG8E6_000971"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFS5061409.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACG8E6_000972"
     CDS             complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACG8E6_000972"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS5061410.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACG8E6_000973"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACG8E6_000973"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS5061411.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7989 c         6706 g        11988 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatcg cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gcaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ccagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 cgtcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc ttcttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc taattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgttcttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gatatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatac
//
LOCUS       DAZUMM010000021        36550 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000021 DAZUMM010000000
VERSION     DAZUMM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.021"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..694
                     /locus_tag="ACG8E6_000974"
     CDS             23..694
                     /locus_tag="ACG8E6_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5061412.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            753..1193
                     /locus_tag="ACG8E6_000975"
     CDS             753..1193
                     /locus_tag="ACG8E6_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061413.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1205..1873
                     /locus_tag="ACG8E6_000976"
     CDS             1205..1873
                     /locus_tag="ACG8E6_000976"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS5061414.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1937..2560
                     /locus_tag="ACG8E6_000977"
     CDS             1937..2560
                     /locus_tag="ACG8E6_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFS5061415.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2616..3002
                     /locus_tag="ACG8E6_000978"
     CDS             2616..3002
                     /locus_tag="ACG8E6_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFS5061416.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3215..4606
                     /locus_tag="ACG8E6_000979"
     CDS             3215..4606
                     /locus_tag="ACG8E6_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFS5061417.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5217..5585
                     /locus_tag="ACG8E6_000980"
     CDS             5217..5585
                     /locus_tag="ACG8E6_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFS5061418.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5765..6706
                     /locus_tag="ACG8E6_000981"
     CDS             5765..6706
                     /locus_tag="ACG8E6_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFS5061419.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6775..7506
                     /locus_tag="ACG8E6_000982"
     CDS             6775..7506
                     /locus_tag="ACG8E6_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5061420.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7650..9830
                     /locus_tag="ACG8E6_000983"
     CDS             7650..9830
                     /locus_tag="ACG8E6_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFS5061421.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9850..10332
                     /locus_tag="ACG8E6_000984"
     CDS             9850..10332
                     /locus_tag="ACG8E6_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFS5061422.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10348..11109
                     /locus_tag="ACG8E6_000985"
     CDS             10348..11109
                     /locus_tag="ACG8E6_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS5061423.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11240..12574
                     /gene="murC"
                     /locus_tag="ACG8E6_000986"
     CDS             11240..12574
                     /gene="murC"
                     /locus_tag="ACG8E6_000986"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFS5061424.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12881..13366
                     /locus_tag="ACG8E6_000987"
     CDS             12881..13366
                     /locus_tag="ACG8E6_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFS5061425.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13359..14042
                     /locus_tag="ACG8E6_000988"
     CDS             13359..14042
                     /locus_tag="ACG8E6_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFS5061426.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14168..16813
                     /gene="polA"
                     /locus_tag="ACG8E6_000989"
     CDS             14168..16813
                     /gene="polA"
                     /locus_tag="ACG8E6_000989"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFS5061427.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16858..17694
                     /gene="mutM"
                     /locus_tag="ACG8E6_000990"
     CDS             16858..17694
                     /gene="mutM"
                     /locus_tag="ACG8E6_000990"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFS5061428.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17658..18287
                     /gene="coaE"
                     /locus_tag="ACG8E6_000991"
     CDS             17658..18287
                     /gene="coaE"
                     /locus_tag="ACG8E6_000991"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFS5061429.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            18339..18671
                     /locus_tag="ACG8E6_000992"
     CDS             18339..18671
                     /locus_tag="ACG8E6_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS5061430.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18983..19474
                     /gene="nrdR"
                     /locus_tag="ACG8E6_000993"
     CDS             18983..19474
                     /gene="nrdR"
                     /locus_tag="ACG8E6_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFS5061431.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19497..20870
                     /locus_tag="ACG8E6_000994"
     CDS             19497..20870
                     /locus_tag="ACG8E6_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS5061432.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20870..21796
                     /gene="dnaI"
                     /locus_tag="ACG8E6_000995"
     CDS             20870..21796
                     /gene="dnaI"
                     /locus_tag="ACG8E6_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFS5061433.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21925..22365
                     /locus_tag="ACG8E6_000996"
     CDS             21925..22365
                     /locus_tag="ACG8E6_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS5061434.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22509..23210
                     /locus_tag="ACG8E6_000997"
     CDS             22509..23210
                     /locus_tag="ACG8E6_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFS5061435.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23207..24328
                     /locus_tag="ACG8E6_000998"
     CDS             23207..24328
                     /locus_tag="ACG8E6_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFS5061436.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24343..25575
                     /gene="pepT"
                     /locus_tag="ACG8E6_000999"
     CDS             24343..25575
                     /gene="pepT"
                     /locus_tag="ACG8E6_000999"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFS5061437.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25707..26615
                     /locus_tag="ACG8E6_001000"
     CDS             25707..26615
                     /locus_tag="ACG8E6_001000"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5061438.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26650..26922
                     /locus_tag="ACG8E6_001001"
     CDS             26650..26922
                     /locus_tag="ACG8E6_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFS5061439.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26933..27979
                     /locus_tag="ACG8E6_001002"
     CDS             26933..27979
                     /locus_tag="ACG8E6_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFS5061440.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28230..30839
                     /gene="clpB"
                     /locus_tag="ACG8E6_001003"
     CDS             28230..30839
                     /gene="clpB"
                     /locus_tag="ACG8E6_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFS5061441.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30990..31676)
                     /locus_tag="ACG8E6_001004"
     CDS             complement(30990..31676)
                     /locus_tag="ACG8E6_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061442.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31654..32148)
                     /locus_tag="ACG8E6_001005"
     CDS             complement(31654..32148)
                     /locus_tag="ACG8E6_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS5061443.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(32168..33388)
                     /locus_tag="ACG8E6_001006"
     CDS             complement(32168..33388)
                     /locus_tag="ACG8E6_001006"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5061444.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33535..35370
                     /gene="lepA"
                     /locus_tag="ACG8E6_001007"
     CDS             33535..35370
                     /gene="lepA"
                     /locus_tag="ACG8E6_001007"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFS5061445.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(35464..>36550)
                     /locus_tag="ACG8E6_001008"
     CDS             complement(35464..>36550)
                     /locus_tag="ACG8E6_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5061446.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        12164 a         6464 c         7816 g        10106 t
ORIGIN      
        1 tgatgaaaaa agggggagaa aaatgaattt tttagttgtt ttaaaagata ctctgattga
       61 gcgatctaca tttctatatg ttcaattgca ggaaaataaa acattgctgc atttttcacc
      121 agagttccat ctagaggggc taacattagg agaaatctat caagcagaag ggctgtcggc
      181 tgttttgcac ttttttaagg tagaattgga attaccagta aaagattata tcgagctttc
      241 tcttgaatct tgggatcgag cagtggttga actgtttcca gaaggtttaa tgatcgatac
      301 aaacgatggg aagatccatc tgactgcagc agaactgcaa aaacaaatgc gttatcaaat
      361 agatgacgaa aattcttttt ctatttttag acgtcagcaa aagattctaa aaagcttttt
      421 aaaagtgatc agtaaaaaac ggaatctgat caaaacgaca cagctgttga aaaaatatcc
      481 aggtctgctt cacacgtcta ttcaatttcc tcaattgatt acgatgatcc atcgttttat
      541 acgtatgcaa cagctaccat ctaagaagct gtttcttcca ttgacggaca catttcgagt
      601 agtcaacaga gaagataaaa agaaagtgat tgtgattgac tttaccagaa atcggaatgt
      661 ccttcaccaa gttgcaatgg aaaaggccaa ttaaggcttt ttctttttgg atttttttgt
      721 cataataaat attagatgat ggaggagata atatgatgca aacatggtta gaaaggctaa
      781 tacgatttca gcagaactat cacaaactta tgcagggaag atatgcgaaa tttgatcagt
      841 tgaatcgcgg tctattgatc gctgcgcttg tctttagttt gctaagtcgt tggttgccct
      901 atagaatcgg acaattgttg ttcatcgtct ttttcggatg gatgatattt cgtttcctat
      961 caaaaaaaat ctaccccaga ctaaatgaga atcaacgtta tttgaaatat cttgatagag
     1021 tcaaagaaaa atatcatcag ataaaaaaca aaaagcaagt caaaatggat cgaaaaagtt
     1081 atactttttt tgagtgcccg aattgtcatc aaaagcagcg tgcacctaaa ggaaaaggac
     1141 gaatacgtgt gacatgcaaa acatgtggta taaaattcga aacgaatgtt taagggagaa
     1201 aagaatgaaa ttagtattat tacgtcatgg cgaaagcgaa gcaaattttg aaaattactg
     1261 gacaggatgg ctagatgtcg ccttaacaga aaaaggaaaa gaacaagcag cagttgccgg
     1321 tcaaaaaatg cttgaagccg gacttcaatt cgatgccgtt tatacctctg ttttgaaacg
     1381 tgcgattctt accggacaag tcgcactaga agaaatgggt cagttatggc ttcctataat
     1441 aaaaacatgg cgtctaaatg aacggcatta tggggatctt gtgggtaaaa acaaagataa
     1501 gatgaaagaa atatacggaa aagatcaagt aaaaacatgg cgcagaggat atacggaagt
     1561 tccgccgtta accgcagaaa atagatttga ccgcagatat gatcacttgg atccgcattt
     1621 gatcccatac ggagaaagtc tggaaatgac agtgaatcgg atcattccct tatggcaaga
     1681 tcatctagca ccaaaactaa aagatcggca agatttacta gttgtgggtc atggtaatag
     1741 catccgagca ctgacaaaat atttagaaga tgttcctgaa gatcaaatgg atacgatcga
     1801 tatcccaaat gcacaaccaa tccagtatac attagcagat gatttgacaa ttctggacaa
     1861 aaaaatactt taaagaacac ctttatctaa aagtgagaga tagaaaaaag aagcaaagga
     1921 atggaacaaa aggaacatga aaaattatat atgggacttt gatggtacat tgtttgatac
     1981 gtatcccgca atggtagatg gtgcatggca agcactaaaa gattttggta tcagtatgga
     2041 caaaaaagaa atctacttca aaatgaaaaa gtattctact agttatttga tcaatgaatc
     2101 aaacttgaat gccagagagt ttaatgaatt atttcaccga tatgaaaaag aaagtacgga
     2161 agtttcccgt ccatttccag aaacgaaaca agtactggaa atgctaaaag acaacggcgg
     2221 tcgtcatttc atcctgaccc accgtttgac agaatcaaca tggggcctgt taaaagaaca
     2281 tcgattggca catctgattg aggaagtagt agggatcgat caggactttc ctcgtaaacc
     2341 tgatccagca tctttaaatt atttgatcga tacttttcat ttagaaagaa cagacacgat
     2401 gatgatcggc gatcgtcgtt tagatatcga agcagggaaa aatgcaggag tcgctacttg
     2461 tctttacgac atcgatcatt ttctgggtga gatcccggca gattatgtgg taggaaatct
     2521 gaatgaaatc cttaccttgc agagcatcat taaaaagtag tatccccttt caaaggagat
     2581 atgttataat tttcataaag gagtgataaa attatatgga aatcagaaga caaaaagaag
     2641 ttgtagaagc atttcattat gatatgagat tgccgaacca agaagtagaa acagatttga
     2701 aagtaggctt ctcgccttta aaagaaacga tcgaaaacta tccgaaagaa catagcatcg
     2761 taggagcacg cttagagttc cgtttagtgt ttgatacata tgttttatct ggaagtgtga
     2821 gtcagatcaa tcatatcctc aatcggaaaa ttgaaggtca aaatgatgtc acacaagaag
     2881 aagtggacga gctagtagca ccgttattcg atatcgtacg ccgaatgtct tatgaggtga
     2941 cagagatcgc cttagatcag cctggggtaa aattgaattt tcaatcagaa aaacaagaat
     3001 agacagtaaa aacatacctg cgatagatta aagctatcac aggtatgttt ttttattact
     3061 aattgtaagc aaaaagaatg tcatttcgat aaaaatcgtc taaaatataa aagtcactga
     3121 aaaaaatttt ttcaggagga tggttgaaga atcattctcg ggaaccttta cttccttcga
     3181 tggacttgtc attaataata tgaggaggag aaaaatgaca acactcatca cagttttagt
     3241 ggttctagtt tttgcggcat tattatatgt gttctatcgt atgcaaaagc gccatacaaa
     3301 attttcaaca cgcgtcttta cagcattagg agttggtatc gtctttggcg gtctgcttca
     3361 attgatcttt ggtgtagacg acaaagtcgt tactcaatca atggaatgga tcgggattgt
     3421 aggtcaagga tatgtatcat tcttgcaaat gctagttatg cctttagtgt ttatttcgat
     3481 cattggtgca ttcacaaaaa tgaaagtttc tgaaaagcta gggaaaatta gtgcgactgt
     3541 attgacttct ttacttggaa caacagctat tgcagcattg atcggaattg gttcaacaat
     3601 gctgttcgga ctccaaggag ctaaattcac acaaggagct gcagaaacag cacgaatctc
     3661 tgaactggca acacgtcagg aaacagttga aaacttatcg attccacagc agattttatc
     3721 ctttatccct aaaaacgttt ttgctgattt agcaggtagt cgtgcaacaa gtacgattgg
     3781 agtcgttatt ttcgcggcat ttgtcggaat cgcttatcta ggcgtgaaaa gaaaagcgcc
     3841 aaaagaagca gcattctttt cagatttagt cgacagtctg tataaaatca ttatgcggat
     3901 cgtgacgctt gttttgcgtt tgacacctta tggagtattt gctttgatga caaacgtttt
     3961 ggcaacaagt gattttgcag ctatcgtcaa tttaggtaaa tttgtcgttg cttcttacgt
     4021 agcattgctt gctgttttga ttgtccactc actgattttg attggtgcaa aagtcaatcc
     4081 aatcaactat ttcaaaaaag cctttccggt attgagcttt gcgtttactt cacgttcaag
     4141 tgctggagct ttgccattga atatcgagac tcagacaaaa gctttaggag tagatgatgc
     4201 aacagctaat ttctcagcaa gttttggttt gtctattgga caaaacggct gtgctggcgt
     4261 ttatccagct atgttggcaa cgatcgtcgc accagcagct ggagtgaatg tctttagttt
     4321 ggaatttatt ttgatgcttg tggcaattgt aacaatcagt tcttttggtg tagctggagt
     4381 cggtgggggc gcaacatttg cttcactgat cgttttaggg gcaatgaact tgccagtagc
     4441 aatcgttggg ttagttatct ctgttgaacc attaatcgat atggcacgta cagcagtaaa
     4501 cgtcagcggt agcatggtag ctggtatcgt gacaagtgca cgcatcaaag atttagatcg
     4561 cgatgtcatc aatgatgatt ctaaagtgat cgatgtgaat gcatagtcaa aaagaggttg
     4621 tgatagaagt ggtcagcttt aagaaataag aagaagtttc ctaaaaatag tttctcaaat
     4681 ttttgtgaaa tttcggctta tttccgagaa gctacttctg aaacaccgtg aataagaggt
     4741 tgtgaaaaag ctgccctgca tcaagtaata agaacagcca aacaaaaata gttcctcata
     4801 ttttacgttt ggttgagctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
     4861 aggtcgtgag cctgccgttt cactctgagt cacaaagagc aatttcaaaa aatagcttct
     4921 catgtttgtt gaatattgtg tcttgtgacc gaggagttgt caggagaaca ccgtttacca
     4981 gaggttgtga cttgttttta gtcacgacct ctttttttat ttattccata taaatacaca
     5041 ttatttattc cgaaaagtga aagcgcaaat aacaaaaaca accaacttat ttattattaa
     5101 agaaaagcgt atttaagtag gacgattctg ttgagagctt tcgtagattt tcccattttc
     5161 ctatggtaaa ctgacaaagt tgagaatgtt agaacagatc gtttaggagg aaacagatgt
     5221 ttacgaatga aataaaaatc atgctttatg tggacgatgt agaaaaaaat gcaacgtttt
     5281 ggagagcagt tggctttgcg gaaaaagatc gtcaagaaat ggatgggacg cttataattg
     5341 aagtaggagt tgaggacagt caaacctcat ttctgttata tgatcgagca tttgttgagt
     5401 cccattcacc tggcacagct actagtgccc catctttgat gttttacagt aaagatatct
     5461 ttggactata taaaaaaatg cagacgcaat cagtcacatt aggtgatatg gtacagcttg
     5521 gagaagaata tgtctttaac tttgctgatc ccgatggcaa ctactttgcg gttacaggta
     5581 aataatagaa ataaagactt tgtttttctg tgcgaaacag gaaagcgaag tctttttaat
     5641 tcaaataata atattatgta aacaaaaaac tttccatgcg taattataca caagcaaata
     5701 aatcgtcaaa tgcgaacgcc ttctttatac tgtttaggaa cgaaaagaag gaaggaagaa
     5761 atacatggat tatcaagtac tgctttatta caattataca aaaatcgaag aaccagagga
     5821 atttgccaaa aatcatttag ccttttgcaa atctttggat cttaaaggtc gtattctagt
     5881 agctgaagaa ggaataaatg gtactgtgtc tggaaccgtt gaaaacacga ctgcctatat
     5941 gaaagcaatg aaagctgatc ctcgatttgc tgatacgatg ttcaagatcg atccaagcga
     6001 aggacatgcc tttaagaaga tgtttgtaag ataccgtcca gagcttgtaa gtttaaagct
     6061 agaagaggac attgatccaa accaagtgac aggtgcctat ctttcaccgc aagaatttaa
     6121 agaagctatt ttagatgaac agacagtggt gattgatgca cggaatgatt atgaatatga
     6181 cttgggacat tttcgagggg ctgtacgccc agatatacgc agttttcgag agcttccaga
     6241 ctggatccgc gagcacaaag aagaatttat ggaaaaacgg gtagtgacct actgtactgg
     6301 tggcattcgt tgcgagaagt tttctggttg gcttgtccgt gaaggattca aagatgtcgg
     6361 acagctgcat ggcggtattg ctacttatgg taaggatcct gaagtccaag gagaattatg
     6421 ggacggcaag atgtatgtgt ttgatgaacg gatcgctgtc gatatcaacc aagtagataa
     6481 acaaatcgtc ggtcgcgact ggtttgacgg aacaccatgt gaacgatata tcaattgtag
     6541 caatcctgag tgcaatcgtc agattcttgc ttccattgaa aatgaagaaa aacatttagg
     6601 agcatgtagc atagagtgtg cgaaacatcc gcgcaatcgc tatgttcaag accatggaat
     6661 ctctccagaa gaagcagaac tgcggtttac agctttaatt aaatagaaaa caatagtttt
     6721 gattgtcgac catttccaat atgcgctata ctagcgttga ggatggtgat ccacatgaaa
     6781 tcattgattt atgctattgg ggatgtccat ggcaaacatg agctttttga aaaacttatt
     6841 cactattatg atccaaagat ccatcagctt gttttattag gggatctcaa tgatcgaggt
     6901 cctcgtacaa aagaatgttt tttattagga aagcaattag tcgaagaaac aggagccatt
     6961 tacctccggg gaaaccatga agaatatttt ttgcaatttc tgcaatcacc ggaggactgg
     7021 tatgcgcctt atatgagaaa tggtggaaaa gaaacgtttg aaagcctgat tcacccaggg
     7081 gcatcggagg aatactcccc aactgaaatc tcgatgatga tccaatcgag atattcagaa
     7141 ctgattaaat ttttagtcca gcgccccttg tattatgaat gggaaaatta tctttttgtc
     7201 catgccggtg tggatttgac aaagaaagac tggcgtaaga caagtctaag agactttatt
     7261 tggataaggg aaccatttca tgaagggaaa aacaatacag gtaagacaat tgtatttgga
     7321 catacgatta caccaatgct ccatggagat atgcagacaa cagacctctg gattcaggat
     7381 cataaaatcg gaatggacgg tggggctgta ttcggagggt cattgcatgg ggttgttttt
     7441 gacaaggaag gtattcttca agatattgaa tatcagaaca acgaaggacc ttggcatgga
     7501 atctgactaa tggaaagaat gaaggagctg tgactttgtt gcggctcttt ttatttttat
     7561 agagaatatt gtttctttca gtagtttaac ccaggataaa aagtatgata gaataaagaa
     7621 gcaattttta gcagaaaaga ggcattttaa tgacggaaaa tttgaaccag acgatcattc
     7681 agcttgtaca aaaagaactt tcagactatc gtcctaagca gttgactact gtacttaatt
     7741 tgttaaatga aggaaacact gttcctttta tcgcgcgtta ccgaaaagaa atgactggaa
     7801 gtcttgatga agtccagatt cgtgaaatcg aagagcggta tgcctatctt gaaaacttag
     7861 aaaaacgtaa aactgaagtg attcggctaa tcgatgaaca agggaaactg actcccgagt
     7921 tagaagcaga aatcacccaa gccgtgaaaa tgcaacaagt ggaagatttg tatcgtccat
     7981 acaaacaaaa acgacggaca aaagcaacga ttgctaaaga aaaaggactt gaaccactcg
     8041 cgaagtggct gatgcagctt acagatggag aggtgcaatc tgaagcagaa aaatatattg
     8101 acaaagaaaa agaagtctct tctgccgaag aagcgttgca cggtgctcat gagattattg
     8161 ctgaacaagt cagcgataat gcaaaatttc ggacttggat tcggtcttat acgtataaca
     8221 aaggtatgta tgtcagcaat gtcaaggatg agcaagcaga tgaaaaaggc gtatatgaaa
     8281 tgtattatga ctttgcagaa cccgtccata aaatggtttc tcatcgaata ttagctacta
     8341 atcgtggaga aaaagaagat attctgaaag tattctttca agtagatgaa gcagcaattt
     8401 tagcttactt ggatcgccaa atagtcaaaa atcctgcctc tccttcttct tcattcgtca
     8461 gagaagccta tcaggatagt tataaacgct ttattcagcc agcaatcgag cgagaactgc
     8521 gcaatgaatt gactgaaaag gcagatgagc aagcaatcgc tattttcggc gaaaacctcc
     8581 gtaatctttt gttgcaaccg ccactaaaag gtaaagttgt cctgggattt gatccggctt
     8641 atcgtactgg atgtaaacta gcagtcgtag atgcgacagg gaaagtttta gcaatcgaag
     8701 ttatttatcc tcataaacct gcagcgcaag caaaaagaga agctgctgga ccagctttta
     8761 tccaattaat taataaatat caagtagata tggtagctat cggaaatggt acagccagtc
     8821 gggaatctga actttttgtg gctgagcagt tgaaatccgc cgaccataaa acctattacg
     8881 ctatcgtcaa cgaagcaggc gcttctgttt actccgctag cgaaattgca cgaaaagaat
     8941 ttccgcatct acaagttgaa gaaagaagtg ctgtttctat tgcccgtcgc ctgcaagatc
     9001 ctttagctga attagtcaaa atcgatccta aagctgtcgg agtaggccag taccaacatg
     9061 acgtttctca aaaacgtttg gctgaacaat tggattttgt tgtggaaaca gcggttaacc
     9121 aagtaggtgt ggatgtgaat acagcaagcc cgcagctctt gcagcatata tcaggattga
     9181 ataaaacaac tgcccaaaac attgtcattt atcgtgaaga aaacggagaa ttcaccgcac
     9241 gaactcaatt gaaaaaagtg ccacgcttag gaccaaaagc atatgaacaa gcaatcggat
     9301 tcttgcgagt ccctggtggg aaaaacattt tggacaacac aggtatccat cccgaaagtt
     9361 attctattgc aaaagaaata ttaacttcag tccaactttc agaaaaagag cttggtacag
     9421 aagaagcagt agaaaaattg acacgattat ccgtagagaa actggctgaa agcctatcag
     9481 tcggagaaga aacgttagcc gatattttag ctgggctgac acaacctgga cgagatatgc
     9541 gtgatgagat gccagcgccc ttattacgta cagatgtatt gagtatggaa gacttgaaac
     9601 cagggatgga attgactgga acggtccgta atgtcattga cttcggcgca tttgttgata
     9661 tcggtgtgaa gcaagacgga cttgtccata tatcgaaact cagcaaaaaa ttcgtcaagc
     9721 atcctacaga tgtcgtgtca gtaggagata tcgtaacagt ttggatagaa caagtcgata
     9781 cgaaaaaagg tagaatcagt ttgacgatgc tttcccctta tgaagagtaa caatgaggag
     9841 taacaaaaat tgacacaaga agaactgcag cagttagtag aagagatatc attaaaaagc
     9901 ttccagcttc ctttcaagca ccaagcaatg ttcaatccac gcttacgtac aactggcggg
     9961 cgctatcatt taaacgatca tcatttagat tttaatgcga agctatttga agagtcagac
    10021 gaagagacga ttgcaggaat catcaaacat gaactttgcc attatcatct tcatctagct
    10081 ggaagagggt atcagcataa agatatggat ttcaagtatc ttttgaaaaa aacaggaggg
    10141 ttgcgctatg caccagcctt gacgaagaaa cagataaaga tggagtatta tcgatgccaa
    10201 gattgttctg ccacgatctg ccgcaagaga agaatcgatg tacgcaaata tcgttgtgga
    10261 agatgtcatg gagaaatcaa atggattgag acaaaaagag aagactttaa tgaagcacaa
    10321 aaaatcagat gaaaggatag aaggcctttg gaaaaaagtg agcagaggaa tctaaaagac
    10381 tggaatcagc tattaaattt tttagccgca cctgaagagg gaaattttga ggaactgacg
    10441 gcacctacac ttattttagc tggaaactgt cttcctatct tagctgataa agcagcacaa
    10501 atgtatttaa atggccaagt agatcaattg tttttggtcg gaggggtggg gcatgcgact
    10561 agaatccttt atgagaattt tgaaaagcaa ggatttcatt ttgaagatgg catgagtgaa
    10621 agtgaaatct gtcgtcagta tctaaaagaa gtctatgatt tgcccgataa ggcttttctg
    10681 atagaatcga agtctacaaa ttctggagag aacgccactt tttcactgga gattcttcat
    10741 tccttaggag ctgtaccaga aaaaatctta ttgatgaatg atccgacact gcaacgcagg
    10801 actcgagcaa cttttgagaa agtatggcaa aatgaacaaa ctattttcgc gaatgctgtg
    10861 ccatttgtgc cggaaatcct tcacttttct gaagagatca tctttacagc aaaagaactg
    10921 aatcatcaat ggcctaagga atatttccat gcgctagtat taggagaaat ggaacgtctc
    10981 catgacgatg agaacggcta tggtccgaaa ggaaaagatt ttattcctca cattgatata
    11041 tcagaggaag tttggtcttc ttacaccaga attaaagagt caataaaaac ggatttttct
    11101 cgtacttgac cattttagca agcgaatcct cctttccaaa taaatttggt tgaaattttc
    11161 aatcgtttat aagataatga ggagcgtgtt gaatgtaata gctaaaagaa tggacgctag
    11221 ataagagaag aggattcaaa tggaaaatca aaataaattg tatcattttg ttggtatcaa
    11281 aggttcagga atgagctcct tggctcttgt tttacatgaa caaggtctaa atgtccaagg
    11341 atcagacatt gaaaaatatt tctttacaca aagagattta gaaaaagcaa atatcacgat
    11401 tctgccattt aacgcagata atgtaaaacc agggatgacg atcattgcag gaaatgcatt
    11461 tcctgattca catgaagaaa tccagcgagc aaaagaatta ggtttggaag tgattcgcta
    11521 tcacgacttt attggtcatt ttatccaaaa ttatacgagt atcgctgtaa caggctctca
    11581 cggaaaaaca agtacgactg gactgctctc tcatgtattg agtggtgtcc gtccaacaag
    11641 ttatctaatt ggagatggaa caggacatgg tgatccgcaa gcggaattct tttcatttga
    11701 ggcctgtgaa tatcgccgtc atttccttgc ctattcacca gactatgcaa tcatgaccaa
    11761 tatcgatttt gatcatccgg attattatac aagtatcgac gatgtattta cagctttcca
    11821 aacaatggct ggacaagtga aaaaagctat ttttgcgtat ggtgacgatg cttatttacg
    11881 caaattaaaa gcaaatgtgc cgatttatta ttatggtgtc acagaaaacg atgatatcca
    11941 agcacgaaac atcgaacgca caacttctgg ttctgctttt gatgtctatc atggggatga
    12001 atttgttggg cacttcactg taccagcatt cggtaaacac aatatcttaa atgctttagg
    12061 tgttattgca gtagcttatt tcgaaaaact ggatctgaaa gaagttgcag aagaaatgct
    12121 gactttccca ggagtaaaac gtcgattcag tgaaaagatc gtagcagaca tgaccgtagt
    12181 cgatgattat gcgcatcacc ctgcagagat caaagcaacg attgatggcg cacgccaaaa
    12241 atatccagat aaagaaataa ttgcagtttt ccaaccccat acgtttacgc gtacgattgc
    12301 attgatggat gaatttgcag aagcacttga tttagctgat aaagtttact tatgcgatat
    12361 ttttggttcg gctcgggaag aacaaggaaa cgttaagatt gaagaccttg gagcgaaaat
    12421 caaaaaaggt ggagaagtca tcaaagaaaa taatgtatct ccattgttag attatcatga
    12481 tgcagtagta atcttcatgg gtgctggaga tgttcaaaaa tttgaacaag cgtatgaaaa
    12541 acttttaagc agcacgacga aaaatgtact gtaatacaat tactgcagtg tccttataaa
    12601 aatagaaaga gggggagtgg caaaaatctt gccaagcccc cttttttgca acttatgata
    12661 aacggtgttc aaaagcttgc atctgcggta ataagcccaa tcaaatcaaa aacatgagaa
    12721 gctgtttttt cttggctgtt cttattattt gatgcaggac agctttttca cgacctctgt
    12781 aaactatagg aatctgttaa atcaagggag cagaacttgc cttttacgct acttttggta
    12841 caataatgta tgattaaaaa taaactggag ggtttctatc ttgaacatag gtaagccgag
    12901 aaaactagga aaaacaataa atgaaatcga ggaaggcgac tcgttatcct taacagaatc
    12961 tattgaagat aaccagcttt tgctgtattt gggattgacg aatgatgcaa atcctttgta
    13021 tatccaacat gactttgcac aaaaaacaga atatcaacag ccaatcgttc catcagtgat
    13081 gctgatgggg atcatcacaa gtgcaatttc aaagcatctt cctggaccag gatcacatgt
    13141 tgtcaatttt tctgtcaatt ttattgaacc ggttttccat tatgaaacac tgacttttca
    13201 gtttgaggtc ataaaagtag ataagatgaa ggatgtggtg acgatttctg tagaagcgga
    13261 gaatacgaac gaagatcgtg tgttggatgc tgtcgttatg gtccaaccgc cacaagttcc
    13321 tgaattggaa atagcaatcg aaagtgaagg tgaattgaat gaataatcca agtacatcaa
    13381 tgtcaggtat caatcgtttt tatgcaaaag tttatggatt tttaggtatg ggacttgccc
    13441 ttagtgcagt cacttcttac ttggctcttt gggtatttcc tttgcaatta gcaactttta
    13501 tcaataattt tccactaggc tttgtgggtc tgtggatcgt tgaactgatt ttagtaatgg
    13561 ttctaggcgt aaaagcacaa aagaatccga gcttagcaat cggtggattt attgcctact
    13621 ctcttttgaa tggcataaca ttagcggtta ctcttgcata ctacgatatt ggaacagtaa
    13681 cgcaagcttt cttgacagct actttgatgt tcatggggat gtctgttgtc ggagtaacaa
    13741 caaaaagaga tcttagcggg gttggaagag caggacaggc agctttaatc ggagtgatca
    13801 ttgcactagt cttgaacatc ttcattttga gaagtagcgt agttgagctg ttcatctccc
    13861 tactgttagt cgtcatcttt gcagggatta cagcttatga taaccaaagc atccgtaata
    13921 tctatgtcca gacaaacgga acagcacata gtggaattgc tgtattcatg gcattgaagc
    13981 tgtatttgga ctttatcaac ttgttcttgg cgtttctgag aatctttgga cggaacaact
    14041 aaaaaagaaa aaaattcatc agttggggct ataccgaatt atttggtatg gtcccgcttt
    14101 ttcttttcgc attccgcagg attctttgtt agaatgaata agaactttac agtaaggagt
    14161 taagaagatg acaaaaaata aattattact cgttgatggg aacagtgtgg cttttcgcgc
    14221 attctttgcg cttcacaatt ctcttgaacg tttcaaaaat agaaacggtc ttcatacaaa
    14281 tgcaatctat gcattcaata cgatgtttga aaatgttatg caaaaagagc aaccgactca
    14341 tgttctggtt gcgttcgatg caggaaaaac gacttttcgg acagaaatgt atgcggagta
    14401 caaaggtgga agatccaaaa cacctggcga attcaaagaa caaatgccat atatacgtga
    14461 tttgttgaca ggactagggg tacagtatta cgaattgccc aattatgaag cagatgatat
    14521 tatcggtact ttggcagaaa aagtagacaa agatcaattt gatgtcgtcg tgctttcagg
    14581 tgatcgggat ttgactcaat tagctagtaa agaagtaaaa gtggatatca ctgtaaaagg
    14641 tgtcagcgat atcgaaagct atacaccaga acatgtagcg gaaaaatatg atgggttgac
    14701 gccaaaacag attattgaca tgaagggact agccggagat gcctctgaca atatccccgg
    14761 tgtgactaaa atcggtgaaa aaacagctat caagctattg aaacaatatg gctcagtcga
    14821 aggaatttat gaacatatcg atgaaatgaa gcaaagcaaa atgaaagaaa atctgataaa
    14881 tgacaaagag caggctttct tatcgaaaaa attggcgacc atcaatactt cagcgcctgt
    14941 ggatgtctct atcgactcat tgaagtatga agggaaaaat ctcgataaac tcgttccgtt
    15001 ttataaagaa atggatttca atcagttcct ttctaaattg aatatcgtag aagaagaagt
    15061 aaaaatggat gatattttgt ttgaagtcgt tcatgaattc aaagaggaaa tgtttactac
    15121 ggatatggcg ttgtacgtgg aaatgatggg cgacaattat catacagagg aaatcgtcgg
    15181 agtagcgtgg gggacagaaa agaaaattta tgtcacgaac gatctatctg tattcgaaaa
    15241 ccgagcgttc cattcttgga tcactgatcc gactcggctg aaaaaagtat atgatgcgaa
    15301 acgaacttat gttgctttaa accgctatac tggaaaaacg aaaggaatcg attttgatgt
    15361 cttgctaggt gcataccttc tcgatacgaa tgaaaaaagc acagatatcg aaggaatagc
    15421 tgctcattac ggatacaacg atatccaatc agatgaagct gtatacggca aaggggcgaa
    15481 aaaaggttta ccagaagaag aggaactctt ttttgctcat ttagcgcgaa aagtagctgc
    15541 aatcaatgcg ttgacaccaa aattgagcca agaactggca gaaaaaaacc aagaagatct
    15601 tttccggaaa atggaactgc cattgtctat cattttggca gagatggaaa taagcgggat
    15661 caaagtagat gcgactcgct tacaagaaat gaagggcgag ttctctgctc gcttaagaga
    15721 gatcgaacaa aaaatctacg aagaagccgg cgaagaattc aatttgaatt caccaaagca
    15781 attaggagtg atcttatttg aaaaaatggg attacccgtt atcaaaaaaa caaaaactgg
    15841 atattccaca gccgtagatg tactggaaca gcttcgtgaa caagcaccaa tcgtagagga
    15901 tatcctcacc tatcgtcaaa ttgcaaaaat tcaatctacc tatgtcgagg gactgctgaa
    15961 agtgatccaa tcagatggaa aagtgcatac ccgttatgtc cagacattga cccaaacagg
    16021 acgattaagc tctgttgatc ctaatttgca gaacatcccg attcgattag atgaagggag
    16081 aaagatacgt caggcatttg ttcctagaga aaaagactgg ctgatttatt cttctgacta
    16141 ttcacaaatc gagttacgcg tacttgcgca tatctcaaat gatgaacatt tgaaagctgc
    16201 cttcctagaa ggtcaagata tccatgccag cacagcgatg cgtgtatttg gaatcgaaaa
    16261 ggcagaggat gtcacaccaa atatgcgtag gcaggcaaaa gcagtgaatt ttggtatcgt
    16321 gtatggcatc tctgattacg gactttcaca aaatctagga atcagccgaa aagcagcaca
    16381 gcaatacatc gatacgtact ttgaaaaata cccaggtgtc aaagaatata tggaaagaat
    16441 cgtacgcgaa gcaaaagacc aaggctacgt cgaaacgctg tatcaccgcc gtcgatatct
    16501 gccagacatc aattcaagaa attacaatat ccgttctttt gcggaacgaa cagctatcaa
    16561 tacaccaatc caaggaagtg ctgctgatat tttgaaaatc gcgatgatcg aattggataa
    16621 acgattaaaa gaaacaggtc ttcaagctac gatgctgtta caagttcatg atgaacttgt
    16681 cttcgaggta ccggaaaaag aacttgaatc attagataaa ttggtgaagg aagttatgga
    16741 acaagctgtt tctcttcatg taccactgat cactgacagc agttggggaa aaacgtggta
    16801 cgaagcaaaa taaacattgt ttaagacaga gcaatataat gaaggtgaaa agaaaaaatg
    16861 ccagaattac cagaagtaga aaccgttcgt aaaggattgg aacggttagt agtaggaaaa
    16921 acgattcaaa aagttcaggt cttgtggccc aagatcatcg agcaaccaga gacacccatc
    16981 tttgaggctt ctcttgttgg cgaaaccatc caatccattg gacgtagagg gaagttttta
    17041 atttttcatc tcgatcattg tgaactgatc tcacacttgc gaatggaagg aaaatatcaa
    17101 tttacgaaag aaaatacacc aatcgataaa catacgcatg tacttttctt ttttgaggat
    17161 ggaagccaac ttcgctacaa cgatgtacga aaatttggtc gtatgacaat cgtcgaaaaa
    17221 ggagcaagtg caacttaccg aggaatcatg aaattgggac ctgagccttt gccagatagc
    17281 ttcttattag cagatttcgc aaatggactg aaaaagtccc acaaagcaat caagccattg
    17341 ctgctagacc aacgtctagt gactggtcta ggtaatattt atgtagatga agcattatgg
    17401 gaagcgaaga tccatccaga acaaccggcg aacacactgc gccccaaaga ggtagaacaa
    17461 ttacgtctat cgatcattga tgttttggat cgtgcaatag aagccggagg taccacgatt
    17521 cgcagttatt tgaatgcatt aggagaatca ggcggatttc aagtagcact gcatgtctat
    17581 cagcaaactg gcaaaccatg tatacgttgt ggaacgccga tcgtgaaaac aaaagtggca
    17641 caaaggggga cgcactattg tccgaattgt caaaggctaa aaatcgcaaa gtaggatttg
    17701 ttttaggttt gacgggtgga atagctaccg gtaaaagtac ggcagccaaa gtctttcagt
    17761 ctcatggatt tcctttgatc gatggcgatg tgattgccag agaaactgtt gagcctggaa
    17821 cgcttgctct gaggaaaatt gtatcagtgt ttggccaaga ggtattgcag cctgatgggc
    17881 aactagatcg agggaaactt gggatgattg tttttccatc aaaggaaaaa cggcaaaaac
    17941 ttgatcagct attagatccg tttatccgaa aagcgataaa agatcaaatc gcctctctaa
    18001 gttccaatca tcctttagta attgtagata tcccattgct ctacgaaggt cattatgacc
    18061 actatatgga tgcagtagct gtcgtttata ctacgccaga aactcagctt cagcgtttga
    18121 tgaaacgtaa tcaactgacg ctagaacaag cgcagcagag aattagtagt caacttccaa
    18181 tcgaagaaaa aaagcagcgt gcggatattt tgttcgacaa caatggaaca aaagaaaaac
    18241 ttgtgggaca aatcgaaaat tggctttttg ctaatcgctt tctttgagta tctgtgtcaa
    18301 ttttgctatc ctgagtatat agaaagtgag ggatagcgat gggatcatta aaaaatcaaa
    18361 aggataaagt cgtaggtaac gcaaaagaaa aaatcggcga gatgctcaat aaagaaaatc
    18421 taatcgatga aggtcgtacg caatcagaaa cagcagaaga aaaagaaaag cagtaccaag
    18481 caagaatcga tgaaatagaa gaccagcagg cagaagaaga agcgaagcgg caagatttga
    18541 ctcaagaacg aaaaaatccg acacctgatc gtagtaaaac accgctccca gaaagtgacc
    18601 acattcccga tgagcaaaaa atcgaggaac agcgaatgaa acactataca ggaatcgaag
    18661 agaagttata aaatagtaaa aaatcgagat gtgacttgaa gcagatgaga aggtgttcat
    18721 ctgcttcaag ttttttgtgc tagatgacta aatcagcttt tgtgtaagtt atttctcagt
    18781 aatcaaggtg atcagtattg gaaaattgaa atttagtaag gacaggggac acaattacgc
    18841 atgaaagtag aaaaaattgt tcttatatcc gtgttttttg ggtaggacag tcgcgtttgt
    18901 gatttgtttc gtgttataat acaactagtt gtaaagaaag acacatcctt tatataaaat
    18961 gcgatgcaag aggtgagaca aaatgagatg tcccagatgt caacataata attcacgagt
    19021 aatcgacagc cgtcaagcag atgatggacg tgcgatccgt cgccgtcgcg aatgtgagaa
    19081 ttgttcgttc cgattcacta catttgaacg aattgaagct gctccgttat tagtaatcaa
    19141 gaaaaacggg gaacgcgaag agttcaatcg cgataaaatt ttaagaggac tcatccgttc
    19201 agcagaaaaa agacctgtag ctatggaaca aatggaacaa atcgtcaacc atgtcgaaaa
    19261 ccgcgtacgc agtctcggag aaaacgaagt atcgacgaat ctaattggtg aatatgtgat
    19321 ggaagattta gttgatttag acgaaatcgc ttatattcgt tttgcaagtg tttatcgcca
    19381 gttcaaagat atgtctgttt tcttaaaaga gttgcaagat atcgtggata aagcaaaaga
    19441 aaataataaa gagaataata aagaaaacaa ttaagaaaca aaaaggaggc atttccttgg
    19501 aaaatgcttg gaaagagctt caaccaaaaa atatttacca aggaagaaaa gcggctccgc
    19561 tgagagaaga cagcaagaac gccttgctgt atctttatca gccactaatc ggcggagaag
    19621 ccttgagtat ctatttcacc ctgttatcag aaatctctca agaatctggc aaaggtcctg
    19681 aagggatgca tgcagatctg cttagtagtc taggctgcgg tatcccgcaa ttttatgatg
    19741 caagaaaaaa gctagaagga atcggcttac tggatgttta ttttaaagaa gatagtgatc
    19801 tgggacctcg ttttatttat gagctgttag aaccactcat tcctaaagca tttttcaaag
    19861 actctgttct ggcttttctt cttctggaaa aagttggtga acgacgattc cagaagctta
    19921 cccaattctt tgaacctaaa aggcatacag cagagggata tcaaaaaatc acaaaaaaat
    19981 ttttagatgt ttatcgcttc aatgaagcag tgtacgctgc taatcaagaa aacatcgaat
    20041 cgctgaataa atcttttgaa gaatctgaat cagtagcatt acttgaagca gattcgacat
    20101 tcgattggca gtttttgaat gattggctgg caaaaaaaca tattgcaagt cttgatgatc
    20161 caatcatcaa acaagtcgac atgtatcatc agctttacgg tttcgatgaa ttgacacttg
    20221 gcgaactgat cagtcaatct tatgacttta gtgaacaaaa agtatctgaa aaagaactgc
    20281 aaaaagccat tttggcaatg cagagacgtc cggtgaagca aacaaaagaa acagttgcgg
    20341 tttctgaaaa cagtaatgaa ccctcgtcaa acgaacattt gacaaacgca gcgcttcagc
    20401 tgataaaaga agcacagagc attccaccga tgaaatatct ggaagcaatc aagtcagaaa
    20461 aaggcggata tgtttctaaa caagaaaact ggttactgca agatcttgtt tctcaatcag
    20521 gtctttccag tagcgtgatc aatgttttgc tgaattatgt actagtgatc aagaataatg
    20581 ctagcttaaa tgccagttat gtaaatacag tggcaaatga atgggcacaa aagaaaatcg
    20641 ccacagcaga agaagcgatc cagcacattc ggcaaataag taatcaagca aaacaatcaa
    20701 aacaaaagaa gcaaacaaat tatcaaactg gcaagcgaaa tgttcgacgg gaaaagcttc
    20761 ctgattgggt caaccagcca aaagatgaaa aacagatcag cagcgagaaa aaagcagaga
    20821 ttgaccgccg ttttaaagaa tacctggcaa aaaaggaggg tgatatctag tggaagacgt
    20881 aggcaaagaa ttgaagagga tcatcagcaa acgcaattat caagaaaagt atgctgaaat
    20941 gatcgctgaa gtcttaaatg atgcggatgt tcaagctttt ctaagcgcac accaagaaga
    21001 attgacacaa gcggatattg aaaaaagtta cgctaaattg tatgaattcg tgcaagaaaa
    21061 gcgtaaatat caaaaaaacg atccaacgat gatcgctccc ggctatgagc cgaagctgac
    21121 gttgaatttt cattatgtgg atgtcactta tgtaccaacg gaagcattgt tagcacggca
    21181 aagacaagaa gaaatcagaa gccgagtaaa agcaatggat atgccaaaag atattcaatc
    21241 ggcttctttt gaaaactttg aacgtacaga tggacgagga tatgcgagct tagaagccct
    21301 agattttgta gagaaatata ctgctgatcc aaaagggtat cataaaggac tgtatctagt
    21361 aggaagtttt ggtattggaa aaacatattt attaggagca acagcgagag aattagcgat
    21421 tgctggattc accacgacgc tagtccattt tccgacattt gccgttgaga tgaaacaggc
    21481 aatcggaaaa gatcaagtgg cagaaaagtt agatgcagtg aaacgttcac ctattttgat
    21541 gatcgatgac attggtgcag atgcgatgag tagttggata cgagacgaag tattcggtgt
    21601 tatccttcaa tatcggatgc aggaacagtt accaaccttc ttctcatcga attttacaat
    21661 ggatgaattg gaacagcatt tgtccgtcac tcaacgtgga gatgaagagc cgctaaaagc
    21721 gaaacggata atggaacgca tacgttattt gacaaaagaa atcgaaatga ctggtcgcaa
    21781 ccgacgcaat ggttgaacag ttgacaagtc caactgacat ttgacctagt agtaaaagca
    21841 ggtcagcgaa tcattatatg gacagaaatg accggagaca atcaccgcaa caagtaatgc
    21901 agaaacttaa acggaggatt ttttatgagg gctatttttt ctagtatatc tgtagttggt
    21961 ctattttttc ttttagcagg ctgcggtagc gcagatagca acacaggttc tgttgattcg
    22021 attgatgcgg gtcaaacagt aaaaacaagc agtacgaaaa cggagagaca aagcatattt
    22081 gtcggaacga ttgaaagtat cactcaagta gaagcagaag aagaaacgct tcagttgaaa
    22141 ctcgtcgatg tcatggccaa agaagatcca gcgaatatgg gaacgtcatt cagcaatgac
    22201 ggagtgatct taaatgctac tgctgaaaaa ataagcgtgg acataaatga attgaaaatc
    22261 ggtgacaaag tccaatttac attagttgag atgccgatca tgacgatgtc tatcccaccg
    22321 caagttcctg gagattcgat tattgatgtt tcattgaaca aataaccaat aacatttttt
    22381 caaaaataag aaaatgttgt tttatagaac gaaaaggtat attttttaag gagatatggg
    22441 aattttttta ttcctagtct ccttatttta tttggaaaat gctataatta tctagaagga
    22501 gtgaagctat gaaccattta gatttatctc agcgcttagc cgcagtgggt gactttgtcc
    22561 ctgaatccgc tcgtttagca gacattggat cagatcacgc ttacttaccc gttgcattga
    22621 tattaagagg aaaaatcgaa tatgcagttg ctggtgaagt cgttcaaggt ccttatgaat
    22681 cagcacgtaa gcaagtcaaa aaagatggat tagaagaccg tatcaccgta cgccttgcaa
    22741 atggtctaga tgctatccag ccacaagatg gtattacagc tgtcacgatt gcgggtatgg
    22801 gaggttcact gatccgagat atcctagaag ctggcaagtt gaatcaacgc ttaagcggac
    22861 aagaacgttt gatccttcag ccaaacatcg gagagcgtac attacgatct tggctgcaag
    22921 aaaatcagta taaaatccaa tcagaaaaaa tacttgaaga aaataaaaaa atatatgaaa
    22981 tcattgtagc cgaaaaagaa caaaaaccag ttacttacaa tgaaatggag ctgatgttcg
    23041 ggccatttct gcttgaagag aaaagtgaga tcttcaaatc aaaatggaca agagagcttc
    23101 aacaaagaaa agctatccgt gaacagctca aacaagcagc taatcaagag gcacgtcaaa
    23161 aacaaatcga gcaggaaatc caaatgattg aggaggtttt aaagccatga caatcagcgg
    23221 acgcactttc atccaaaagt ttgaaagcta ttgtcctcag tggctagctg aagaaggaga
    23281 cccagtcgga ctgcatatcg gaacattgga taagccaatc caacgagtaa tgatgacatt
    23341 agacgttcgt ccagaagtgg tcgaagaagc aatcaagaaa aaaatcgatt tgctgattgc
    23401 taaacatcct ccaatctttc gtcctgtaaa acggctggtt actgatcagc cacaagaaaa
    23461 aatgtatgca gatttgttga aacatgatat tgccgtctat gcagcacaca cgaacatgga
    23521 tattatttgg gatggactga atgactggtt ttgtgaactt ctcggtattg aagtggagag
    23581 ttatttagta aagactcatg agattcatta taaaaaactg gccgtttatg tacctgtgga
    23641 tcatgcacag aaaatgcgtg aagtattagc tgcagctggt gctggtacac aaggtgatta
    23701 taccggtaca agttttacct cgattggaca cggtcgattt actccggaag ctggtgcaca
    23761 accagcgatt gggaaagtag ggaagacaga acaagttcaa gaggcaaaag ttgaggttat
    23821 tttgccggaa actatcgaaa aacaagtgat tcaagccatg cggtcagctc atccttatga
    23881 ggaacctgct tatgatcttt tcgctataga tgaaccagtc gaaatgtttg ggttaggacg
    23941 agtaggtgaa ttgccgcaag aaatttctat agaggcattt gtagaacaag taaaagaagc
    24001 gttccaacta gatggactga gaatagtcca gccaaaaaat gcaaaatcct ctgtgaagcg
    24061 cattgcgatt tgcggtggaa gcggtgaaaa attttatccg caagcaatcg cccaaagagc
    24121 agatgtctat atcacgggag atatctatta tcatacggct catgatatgc aaagcgctgg
    24181 gttgattgca attgatcccg gtcattatat tgaatcgtta tgcaagcaac gatttatcga
    24241 gaaatttgaa tcttggaaac aagaagaaaa ttgggatatt aactttttcg tttctgaaac
    24301 aaatacaaac ccatttcaat ttaattaaaa ggagagaata ctatgtacga aaatcttttg
    24361 ccacgttttt tacgatatgt caaaacagaa acacgctccg atgcaacaag caaaacgact
    24421 ccatcgactc agacacaagt tgcttttgcg caagaattga aaaaagaatt agaagagtta
    24481 ggcttagctg atgttttcta taatgaagat aacggattcg ttatgggaac attaccaagc
    24541 aatgttgatc atgaagtgcg ttccattgga tttatttccc acatggatac agcagatttt
    24601 aatgcagtag gagtcaatcc acaaatcatt gaaaactatg atggcgagtc tacgatcgta
    24661 ctagatgcgg aagaacgcta tacattgaat acaaaagatt ttccaaattt gaaaaattat
    24721 gaaggccaca cattgatcac aacagatggc agtacattac tgggagcaga tgacaaatca
    24781 gggattgcgg aaatcatgac tgcaatggag atcttgatca ataatccttc tatccctcac
    24841 ggtaccatca aagtggcttt tggtcctgat gaagaaatcg gtgtcggagc ggataaattc
    24901 gatgttgagc aattcgatgt agattttgct tatacagtcg atggtggtcc tttaggtgag
    24961 ttgcagtatg aaacgtttag tgctgctcaa gcaaatatta cgatccaagg gaaaaatgta
    25021 catccaggga cggcaaaaga tacaatgatc aatgctctac agttagcaat cgattttcat
    25081 aatcagcttc cagccgatga agttccagaa aaaacggaag gatacgaagg tttcttccat
    25141 ttgatggcat tggatgggaa tcctgaagaa gcttctatga gttatatcat tcgcgatcat
    25201 gatcgtgaaa aattcgaagc acgcaaagca caaatcacac atatccaaga agagttgaat
    25261 cagcgatttg atcaagaacg aatcaaagta gatatgtatg atcaatatta caacatgaaa
    25321 gaaatcattg aaaaagatat gagcatcgtg gaattagcga aacaagcaat gatcgagctt
    25381 ggtatctctc cagtgatcga accagttcgt ggaggaacag atggttcaaa aatctcttac
    25441 atgggtattc cgacgccaaa tctatttgcc ggaggagaaa atatgcacgg acgattcgaa
    25501 tttgtttctt tacaagtaat ggaaaaagca acagacgtga ttgtaaaaat tgcagaattg
    25561 aacacaaaga gttaaaatcg aaaaagaaca gcttatgaca aggcgttcgt cccaatgatg
    25621 gaatgtcatc tgtctaaggc taaagggcag gacaatggaa ttggttgtcc cgccctcttt
    25681 tttgaagagt aaataggagg taaagaatgg aaaagctgct agttttttat aacaaaagtt
    25741 cagggaaaga tgagggtgag aaattagcac gctggtttca agattatgta aaagaaaagc
    25801 agccagaatt atatgtagaa ctagctgaaa ctggcccatc gattgatgat gcggaattag
    25861 tcaagaaagc agaagccata gaagctgata ctttagtaat tataggtgga gacggcacga
    25921 ttcatcatat cgtccaagcg ttccaaaaaa cattatctca ataccatgtc ggattacttc
    25981 caggtggaac tgtcaacaac cttgcacgtg ttcttgagat tcccctcaaa aaagaagaag
    26041 ctgctgacgt gatcctggaa ggcaatgtac ggaaaataga ctatggtcaa gtcaatgatg
    26101 aagtcattat ctctacactg acaatcggaa tcttagcaga tacagcagtt tggatcagtc
    26161 aagaagaaaa acagcgttac ggctcgtgga tctttatcaa gaagtttttt aagttattgc
    26221 tgaagaagaa aaaatatcat ttggatttag aaacagacga ggagcattgg aaaggaaaat
    26281 cccaactgtt gacagtcacg atgtctaact ccgtgggcgg ttttacaaat tttgacgact
    26341 cagcgaccct agatgatgga aaatttcatg taacgatcgt accttcctta gatattttcc
    26401 gatttatttt ttatttgcct agaatcatga gaggcaaatt ctatacgatc cccggcatta
    26461 cttatttcac agccaaacgc atgaaaatcc aagcgcagga aaaatctgtg ggcactcgaa
    26521 cggatggaga cacgacagat gatcttccag ttgaattatc tgttgtatca aaaggattaa
    26581 cagtttattc accaaagaaa gaagaaaaag cttagaaata gtagttaaca gaaagttgga
    26641 agaagagtaa tggatgtatt gttacacaat cctttgtggt tttacctttt cattgtgaat
    26701 ttttacttat tttgtctgat ggggtatgat aaacatcagg ctagaactag cggctggcgt
    26761 gttcctgaga ataatttgat atttgtcagc ctgttaggcg gtggtttggg aggattgatc
    26821 tctcgaaaag ttttccatca taaaactcgg aaaaagaagt ttactatagg attcgctgca
    26881 ggtgtacttg ttgatttgat tctcatttat tttttccatt aaggagcagg agatgtcgtc
    26941 taaaggaacg aaaactaaaa tcattctgaa tctgcttttg ctgattgtga tttttgccat
    27001 tatttattat ttaatcaatc aatcatttgc agatatcttt gccgaattgc tgtctaccag
    27061 tgttgttgtt ttggcagcgg ctgttatatt agggacagtt taccaaatcg ctgaagggcg
    27121 ctcaatcaaa gaaattgctg aacctttcgc gccgaatttt acaacgaaag atgggttctt
    27181 gacctctagc tatattgcgt tttaccgaat cgtttcgttt ggtacaggaa cgctgatctc
    27241 ggagatttat ttttataaca aaaaaggaat gaagctatcc cagggagcag gggtgacagc
    27301 actccatatg attatgtaca aactggcagt gatcacttat gcattagctg gcttggtcat
    27361 ccaattctct cttttttatt ctaatggtcc atcaatgatc tggtttatca tcgctggtat
    27421 ccttttgacg atcgtgattg ttgccttttt attggcgatg tccgtcagct tgaatgttca
    27481 aatatttttt gtcagcttat cgaataagtt atttaaaagt gagcgtttgc ggaaatgggt
    27541 ggatacatgc aatcagcaga tctactcttt aagggaaacg gtccaaacca ttatccaaga
    27601 caggactgcg ctattgcgga tttatctatg gaatatgtta aaacttgtat tttggtatgt
    27661 acttccttat ttgattctga ttgaaaacca tcctaaaata gattttctac tgaccatttc
    27721 gtttatcagc tttgcagtag tgctttcagg ggttattcct acgcctgctg gtattggttc
    27781 ctttgaattt gtctatttgt tattattcag accagttgtt ggaacagttg acgcagtttc
    27841 ttcgctttta ctgtatcgtt tcagttcctt tatcttgcca tttctctatg gatttgtgta
    27901 tgtgattgct gaacggcgaa gagtgatcaa agaagaaata atagaagtaa aaaaagagaa
    27961 taaagaaaat caagggtagg tcttttataa gatctaccct ttttatcaaa tcaaagaaaa
    28021 acgttatgga taagataatt ttacgtttgt ttggctcttt ttttatatac atacaatcaa
    28081 ttttgttttt ttaagattta tataagtcga gaaaaaaagt caaaaaacta tgtgtatatt
    28141 ttgaccaaaa ttgaccaaag cgatataata gtttttgtaa agatcaaaga aataagtgat
    28201 cttaccttta attaggaggg tatacctgta tgaatattga gaaaatgact acgacgcttc
    28261 aagaagcgat tgctgaagct caacagattg cggtcactcg aaaacatcaa gatatcgaca
    28321 ttgcccatgt atggaaaatc tttttacaat ctaatcattt tggtcgaaat ttctatacgg
    28381 atgccggatt ggatgtcgaa tcttttgaac atgaaattga tcgtctactg gatgagtatc
    28441 cagttgtttc tggtggaaac gtccaatatg gacaaaacct cagccagaat ttctttcgat
    28501 tattgaatga agcagaccaa atacgtgaaa gcttcggcga cgagtttctt tcaacggaag
    28561 tcgttatttt agcgttgatg aaattaaaaa attatccgtt aactgtttat ttgaataaaa
    28621 acggattatc tgaaaaagaa ttgcgtaaaa acatcgaaga gatgagagga ggagaaagag
    28681 tgacctcaca aaaccaagaa gaacaataca aagcattaga aaaatacggc gttgatttag
    28741 tacagcaagt caaaagcgga aaaatggatc caatcatcgg tcgtgatgaa gaaatccgtg
    28801 atgtcatccg tattctttca cgaaaaacaa aaaataatcc tgttctgata ggagaacccg
    28861 gtgtagggaa aacggcaatc gttgaagggc ttgctcaacg tatcgtacgc aaagatgtgc
    28921 ctgaaaattt aaaagataaa acgatttttt cattagatat gggtgcattg atcgctggag
    28981 caaaattccg tggtgaattt gaagaaagac taaaagcagt cttgaaagaa gtcaaaaaaa
    29041 gtgatggacg tatcctcttg ttcatcgatg aaatccacaa tatcgtaggg gcaggaaaaa
    29101 cagaaggaag tatggatgcc ggaaatctgt tgaaaccaat gcttgctcgc ggtgaattac
    29161 atttgatcgg tgccacaact ttagatgagt accgtcaata tatggaaaaa gacaaagcgt
    29221 tggaacgtcg ctttcaaaag gtattagtca aagaaccaac agtggaagat acgatcagta
    29281 ttctgcgtgg attaaaagaa cgttttgaga tccaccatgg tgttaacatc catgacaacg
    29341 cattagtagc agcagctact ttatctgacc gttatattac tgatcggttc ttaccggaca
    29401 aagcaatcga tttgatcgat gaagccagtg caacgattcg ggtagaaatg aactctatgc
    29461 caacggaatt agatcaagtt actagacgtt tgatgcagtt ggaaatcgaa gaagctgcac
    29521 tgaaaaaaga atcagatgat gcaagtaaaa aacgcttgaa aaatttgcaa gaagaattag
    29581 ctgaacttcg tgaagaagca aatgcgatga agatgcagtg ggaaacagaa aaagaagaag
    29641 tcaattctgt ttcagcaaaa cgtgctgaaa tcgataaagc aaaacacgaa ttagaagatg
    29701 cagaaaataa ctatgattta gaacgcgctg ctgtattgcg tcatggtacg atacctcaat
    29761 tagaaaaaga attgaaggaa ttagaagcta aagcaaaaga cagcgagatt aagatggttc
    29821 aagagtccgt taccgaaaat gagattgctc aagtcgttgg cagattaacg ggtatccccg
    29881 taacgaaact tgtagaaggt gaacgagaaa aactaattaa attgaatgaa acacttcata
    29941 aacgagtgat tggtcaagat gaagcagttg atgcggtcag tgacgcggta attcgttcac
    30001 gggctggatt gcaagaccca aatcgtcctc taggttcttt ccttttccta ggaccaactg
    30061 gtgtcggaaa aaccgaatta gcgaaagcgt tagctgagaa cttgtttgac tcagaagatc
    30121 atatggtacg gatcgatatg agtgaatata tggaaaaaca tgctgtgtct cgtctagtcg
    30181 gagctcctcc aggctatgta ggttacgaag aaggtggtca attaacggaa gctgtcagac
    30241 gaaatcctta tacaatcgtc ttgttggatg aaatcgaaaa agcgcatccc gatgtcttca
    30301 atatcttgtt acaagtccta gatgacggac gcttgactga ttcaaaaggg cgagtagtag
    30361 atttcaaaaa tacggttctg atcatgacca gcaatatcgg ttcacagtta ctgctggaag
    30421 gcgtgactgc tgatggaacg attccagaag cagtggctga acaagtcaac acgctattac
    30481 gtggaaactt caaaccagaa ttcttgaacc gaatcgacga cacgatttta tttacaccat
    30541 tgagcctaga caacgtgaaa ggaatcgttg acaaaatggt ggcgcaattg gcacaacgtc
    30601 tggaacatca agaaatcctg ctgacaatca gtgatgaagc caaaacgtgg attgctgaaa
    30661 atgcttatga accagcatat ggtgcaagac cattgaaacg atttatcact aaggaagtag
    30721 aaacaccact agctaaagaa atcgttgcag gacatgttat gcctaaatca aaagtaacga
    30781 ttactttact tgatggacaa cttcatttca aaacagaaga gctagaagaa atcgtctaat
    30841 cttccattga gactataaaa gaaaaaaggg gtctgacaca actagtgtca gacctccttt
    30901 tttcaaataa acagtgttcc agaagtagtt tcttcgaaaa ataagccgaa atttcttgaa
    30961 gctgcccgct tctgtcacaa cctcttttat taggtatttt tcactttttc taatttataa
    31021 ctttcaaagt aacgctcatc ttttttactt tgagaccatg caaacaatcc cattaaggca
    31081 gcacctatcg tatttgtcag caagtcgccc atcgtatcca tcaatgctgc tcgtccgaca
    31141 aacagtgtgc catcagaagc agcaaaacgt tgaagattca tgcctaagaa ctggtcgcat
    31201 aagaattccc agaactccca aaacacgccg caaagtccag caaacgcaaa gccgaataag
    31261 aggaacaacc aaggactggt ttttgatatc tcagcatctt tcatcaagta accaatcaat
    31321 ccatagccaa gagctgtcag gaccattgga ctcaccgcat gtaagatttt gtcccaaaaa
    31381 gagatgatac tgatcaaatg catccctgtg cctaaaaaga cagagatgaa caagaaaaac
    31441 cagtaaaaca aaacgattgc tgctggtatt ttaagtttga aaactttcaa aatgacttgt
    31501 ggcatgaaaa tcaatgcaat ccctaataaa gattccacga taaatgtgat tccttgagaa
    31561 gtcgaatatt tttgtgtgat aaattcataa aaattatagc agacaacgaa tacaccaaaa
    31621 gcaagtaaag ctatttgcac ctttttgatt tctttaatca ctgaaagtcc ctccatctaa
    31681 aaatcttttt acttctgaaa gcaactcaat ttctgaagaa accacttttc cgttcaattt
    31741 gatcaaacct attgtatata agttcaggta gtgaaattga ttttcagctg tttcttgtag
    31801 tgcagcgatt tttttcgggt gggttccgcc ttgctgtcga gtatcagtat ataagccgat
    31861 cactgggatg cctttcgcat aagccacacc gatttccgat gctaccccgg catcgatcgt
    31921 cagcccatct agtaaagcaa tcattaaatc actagccaac actttttctg tgtcagcaag
    31981 agcaatcatt cgactgtctg cataagcagt tttgtcattg attgcttcat tttcttgagg
    32041 aagatagatc gaaagctcag aagagagctc tctgatttct ttaactaact gttggttata
    32101 taacaaatca cttttagcaa acattggtgc ggcaaaataa attttcatct aaacatcatc
    32161 ctttttatca aaaataatcg ctaaagtaag cttccatgtt tggaatgata cgctctagtg
    32221 acttcagtgt ttcttcatct gtttctaacc gttcgattct tgccaaataa gaagcacggc
    32281 gataattcat catcaagcaa gtcaatgttt ggatatccag tcgaacggat tttcctatag
    32341 gttcatttaa gacacggact tgtccctgtt catcccatgt taaagcgaag acaccattat
    32401 tccaaggagc gacaggatct tcaatgatga aatgaaaagc atcagctgtt ccaacaaacg
    32461 gaaaccgttg aaggaattct tttacatcaa cgatccgagc cataaaatat ggttcgatct
    32521 gctctttgat ctgactatct tcaatcaaaa atgctaaagg ctcattttta taaatatctc
    32581 catgtaccca gtagatcatg gaaaaatgtg cggaaataaa gttccataag ccatttcgag
    32641 cttcttgatc aagatagaac atttctttta cgtgaaatat ttcttcagct acccagtaga
    32701 acagaacacc tcttggttca tggttgctat cgtagtagac agcggctgtg cgctcatctt
    32761 cattttcgaa acgccaatac tcttcccaat gaaaatcact gcggaataaa gcaccatggt
    32821 tttgttgggc aaatttgtca taaacggaaa atacgtctgg atgatcaact ggtaatcgct
    32881 caaccatacc acttactggg atttgtttag gtagctgtgt atctctgatt ttaaaggaaa
    32941 gtttatcaga catgatttcc catcctttac gccgatagta ggggatactg taaggataca
    33001 aataagagat ccattgctta ttcttgcgca tattatccaa tgcaacgata attagatctt
    33061 gcatcaatcc atggttagca tattctggat aagttccaac tccagtcaca ccacccattt
    33121 tataacgtgc accgtgaata ttgacttcac aaggatagat agcgatttga gagatcagtt
    33181 tattttcgtg gaaccatccg aacactttcg atacttccaa aatcggtttt ttggaacgga
    33241 taaaagctcg tttattctca aacccgcttt cctcgatgtc tgcttcagtc acttggaaca
    33301 cataggaaag caattcattg aattgatcga tatgttcttg agtgaccggt tttaattcaa
    33361 gattttctct aaaatctttt tgatccattt tttcatctcc atttattagc ttgataacag
    33421 tatagcaaaa ttctacaaaa aaaacttcta tagctgacta attaaaaact ctttaaatta
    33481 ctgcttttcg ttggtataat ggaaagtgct gattatatga aaagaggaga cgctatggat
    33541 ataaagaaaa tgaaagaacg ccaagagcag attcgtaatt tttcgatcat cgctcatatc
    33601 gaccatggga aatctacatt ggccgatcgt attttagaaa tgacgcacac tgtgacgtct
    33661 cgcgaaatgc aagatcagct gttagactcg atggatttgg aacgtgaacg tggaatcacg
    33721 attaaattaa atgctgtaga actcaattac acagcgaaaa atggagaaac atatatcttt
    33781 cacttgattg acaccccagg gcacgtcgac ttcacctatg aagtctctcg aagccttgct
    33841 gcctgtgagg gtgccgtttt agtggtcgat gcagcacaag gaatcgaagc acaaacattg
    33901 gccaatgttt atttagcgtt agacaatgat ttggagatcc tgccagtcat caataaaatc
    33961 gacctacccg cagcagatcc cgaacgtgtc cgtcaagaaa tcgaagacgt aatcggaatc
    34021 gatgctagtg aagctgtatt agccagtgcc aaatcaggga ttgggattga agatattctc
    34081 gagcagatcg tggaatatgt tcctgcacca agtggagact tagatgcacc gctaaaagct
    34141 ttgatctttg actctattta tgatagttac cgcggtgttg ttctaaatgt ccggatcacg
    34201 gacggtatgg tcaaacccgg agacaaaatc aaactgatga gtaatggcaa aacatttgat
    34261 gttacagagg tcggggtctt ctcgccaaaa gcagttgcta gagacttttt gatggtcggt
    34321 gatgtcggtt atatcacagc aagtatcaaa actgtccaag atacacgagt aggggataca
    34381 gtaacgttag cagacaatcc tgccgctgaa gctttaccag gttaccgaaa aatgaatccg
    34441 atggtttatt gtggtcttta tccaattgat acttctcgtt acaacgattt gcgggaagct
    34501 ttagaaaaac tgcaattgaa tgatgctgct ttacaattcg aaccagaaac ctctcaagca
    34561 ttaggttttg ggttccgctg tggattcctc ggacttcttc atatggatgt cgtccaagaa
    34621 cgcttggaac gggaattcaa tctagaattg atcacgactg caccatccgt tatttaccat
    34681 gttaataaaa cggatggtac gactgctaca gtagataacc cggcagattt tcctgaacct
    34741 gtaacgattc aggatgtaga agaaccattc gtcaaagcac agatcatggt cccaaatgat
    34801 tttgtcggtg cagtaatgga actttctcaa cgaaaacggg gagaattcat tacgatggat
    34861 tatttagatg attaccgagt aaatgtcgtc tataatattc cgctttctga aattgtcttt
    34921 gactttttcg acaaattgaa atcaagcacg aaaggctatg cctcattgga ttatgaaatg
    34981 tctggttatc aaaaaagcaa gttagtgaag atggacatct tgctgaatgg agaaaaagtc
    35041 gatgcattaa gctttatcgt tcaccgagac tttgcctatg aacgtggaaa agcaatcgtt
    35101 gaaaaactga aaaaactaat cccacgccaa caatttgagg tgccaatcca agcagctatt
    35161 ggacaaaaaa tcgttgcccg ttcagacatc aaagccttgc gtaaaaacgt cttggctaaa
    35221 tgttatggtg gagatgtttc tcgtaaacgt aaactcttag agaaacaaaa agaagggaag
    35281 aagcggatga aacaaatcgg ctccgtcgaa gttccgcaag aagccttcat ggccgttctg
    35341 aaaatggacg aggacgaacc gaagaaatag caagggtttg tttgaaaaca gtgtaaaaaa
    35401 taaaccatgt ttacatggat ttgccaccgg tttgccaccg gtggtttttt tcggtggcaa
    35461 aaatcaagaa ttctttctaa gattttccat gatatcgacc gtttcatttc tcatcttatc
    35521 tgttacatgc gagtaagtat ccatcgtaat tgaaattctg ctgtggccca atcgttcaga
    35581 gatttctttc atttttgcgc cattttcgag aagaagtgta gcatgagtat gtctgagaga
    35641 atggaagtta aaagagaggg agagtgcatt cgatatcctc ctagtattcc atttcactac
    35701 acttggcgta actagctcac cgtcttcctt cgtacatact gcatttgaat caatgtagag
    35761 ctttccatac ttcattcgat tttctaattg ttgtttctta tgttttttca gaattgctag
    35821 caaggtttgt ccaataaaaa tcgttcgatt ggaactgctt gtctttggcg taccatatac
    35881 ccatgcgccg tcattcttta ccatttgttt ctctacagta attgttccat ttgaaaaatc
    35941 gacatcatcc cacgtcagac cacaaacttc gccaacgcgc attcccgtat aaaatccaat
    36001 attcaaagga atatagaaag gatgtccttc aggagtaatt tctagcatat gatcaaagtc
    36061 ctcaagagaa atgattttta gatctttttt agtcgttggt cgtgcttcat attttggtat
    36121 ttttacatac agcataggat tttgcttgat taatccccaa ggataaaccg ccatattcag
    36181 cgcattctta aggacagagt gagtaatagt cattgttttc ttcgagtaac cctttttaaa
    36241 ttcagcattg atgaaatttt gtaaaagagc aggggagaga tccgtaagtt ttttctttcc
    36301 taaataaccg tttatatgat ttttgatggt aaatcggtag ttttcatagg tattgtattt
    36361 tagatttagt ttaacgtatt cctccatcca aaaatcaagg tattgtttta ctcgagtatc
    36421 cgtacctaaa aagtattgtc ctgtttcgtc aatatctgat aaaacttttc gtaaagcagc
    36481 ttcggcctct ggtcgggtgt ctccgccaac tttctccact ttttttcttg agccatcatc
    36541 attgatatct
//
LOCUS       DAZUMM010000022        34733 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000022 DAZUMM010000000
VERSION     DAZUMM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.022"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..911)
                     /locus_tag="ACG8E6_001009"
     CDS             complement(63..911)
                     /locus_tag="ACG8E6_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5061447.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1133..3076)
                     /gene="thrS"
                     /locus_tag="ACG8E6_001010"
     CDS             complement(1133..3076)
                     /gene="thrS"
                     /locus_tag="ACG8E6_001010"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFS5061448.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSRENK"
     gene            complement(3413..3592)
                     /locus_tag="ACG8E6_001011"
     CDS             complement(3413..3592)
                     /locus_tag="ACG8E6_001011"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFS5061449.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3639..5462)
                     /locus_tag="ACG8E6_001012"
     CDS             complement(3639..5462)
                     /locus_tag="ACG8E6_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5061450.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHAHSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5740..8019
                     /gene="helD"
                     /locus_tag="ACG8E6_001013"
     CDS             5740..8019
                     /gene="helD"
                     /locus_tag="ACG8E6_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFS5061451.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETEIDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8112..8759)
                     /locus_tag="ACG8E6_001014"
     CDS             complement(8112..8759)
                     /locus_tag="ACG8E6_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061452.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNNVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8938..9657)
                     /locus_tag="ACG8E6_001015"
     CDS             complement(8938..9657)
                     /locus_tag="ACG8E6_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5061453.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9788..10939)
                     /locus_tag="ACG8E6_001016"
     CDS             complement(9788..10939)
                     /locus_tag="ACG8E6_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFS5061454.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKTTGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTYSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDVGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11156..11896)
                     /locus_tag="ACG8E6_001017"
     CDS             complement(11156..11896)
                     /locus_tag="ACG8E6_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5061455.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEIFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11900..12256)
                     /gene="rsfS"
                     /locus_tag="ACG8E6_001018"
     CDS             complement(11900..12256)
                     /gene="rsfS"
                     /locus_tag="ACG8E6_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFS5061456.1"
                     /translation="MIDSKEQLKIAVEAADSKRAEDIIALDIRNVSLLADYFMICSAN
                     SERQINAITEEIIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERSFYNLE
                     KLWSDAPLVDLSEWLD"
     gene            complement(12280..12873)
                     /gene="yqeK"
                     /locus_tag="ACG8E6_001019"
     CDS             complement(12280..12873)
                     /gene="yqeK"
                     /locus_tag="ACG8E6_001019"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFS5061457.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDVEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12857..13507)
                     /locus_tag="ACG8E6_001020"
     CDS             complement(12857..13507)
                     /locus_tag="ACG8E6_001020"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFS5061458.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13517..13828)
                     /gene="yhbY"
                     /locus_tag="ACG8E6_001021"
     CDS             complement(13517..13828)
                     /gene="yhbY"
                     /locus_tag="ACG8E6_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFS5061459.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13844..14953)
                     /gene="yqeH"
                     /locus_tag="ACG8E6_001022"
     CDS             complement(13844..14953)
                     /gene="yqeH"
                     /locus_tag="ACG8E6_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFS5061460.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNELQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14950..15477)
                     /locus_tag="ACG8E6_001023"
     CDS             complement(14950..15477)
                     /locus_tag="ACG8E6_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFS5061461.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15704..16489)
                     /locus_tag="ACG8E6_001024"
     CDS             complement(15704..16489)
                     /locus_tag="ACG8E6_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFS5061462.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRFDQHELLEKRYKRFRQY"
     gene            complement(16482..17351)
                     /gene="accD"
                     /locus_tag="ACG8E6_001025"
     CDS             complement(16482..17351)
                     /gene="accD"
                     /locus_tag="ACG8E6_001025"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFS5061463.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17352..18731)
                     /locus_tag="ACG8E6_001026"
     CDS             complement(17352..18731)
                     /locus_tag="ACG8E6_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFS5061464.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFFSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDHF
                     GNVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18739..19158)
                     /gene="fabZ"
                     /locus_tag="ACG8E6_001027"
     CDS             complement(18739..19158)
                     /gene="fabZ"
                     /locus_tag="ACG8E6_001027"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS5061465.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19183..19659)
                     /gene="accB"
                     /locus_tag="ACG8E6_001028"
     CDS             complement(19183..19659)
                     /gene="accB"
                     /locus_tag="ACG8E6_001028"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFS5061466.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPETMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19662..20897)
                     /gene="fabF"
                     /locus_tag="ACG8E6_001029"
     CDS             complement(19662..20897)
                     /gene="fabF"
                     /locus_tag="ACG8E6_001029"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFS5061467.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20916..21653)
                     /gene="fabG"
                     /locus_tag="ACG8E6_001030"
     CDS             complement(20916..21653)
                     /gene="fabG"
                     /locus_tag="ACG8E6_001030"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFS5061468.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21665..22582)
                     /gene="fabD"
                     /locus_tag="ACG8E6_001031"
     CDS             complement(21665..22582)
                     /gene="fabD"
                     /locus_tag="ACG8E6_001031"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS5061469.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22689..22913)
                     /locus_tag="ACG8E6_001032"
     CDS             complement(22689..22913)
                     /locus_tag="ACG8E6_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS5061470.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22966..23928)
                     /locus_tag="ACG8E6_001033"
     CDS             complement(22966..23928)
                     /locus_tag="ACG8E6_001033"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFS5061471.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23938..24378)
                     /locus_tag="ACG8E6_001034"
     CDS             complement(23938..24378)
                     /locus_tag="ACG8E6_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5061472.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24684..25649
                     /locus_tag="ACG8E6_001035"
     CDS             24684..25649
                     /locus_tag="ACG8E6_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS5061473.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRAQLPDLQ
                     EMTFEQKKKELQGLFQGQNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKHFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMIASADVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQSEIELLNKKEAEKQLN"
     gene            complement(25706..25936)
                     /locus_tag="ACG8E6_001036"
     CDS             complement(25706..25936)
                     /locus_tag="ACG8E6_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFS5061474.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26022..26909)
                     /locus_tag="ACG8E6_001037"
     CDS             complement(26022..26909)
                     /locus_tag="ACG8E6_001037"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5061475.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26998..28221)
                     /locus_tag="ACG8E6_001038"
     CDS             complement(26998..28221)
                     /locus_tag="ACG8E6_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS5061476.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28238..29284)
                     /locus_tag="ACG8E6_001039"
     CDS             complement(28238..29284)
                     /locus_tag="ACG8E6_001039"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS5061477.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYSIRVPIQAISNGIDLEKYRPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29448..31175)
                     /gene="ptsP"
                     /locus_tag="ACG8E6_001040"
     CDS             complement(29448..31175)
                     /gene="ptsP"
                     /locus_tag="ACG8E6_001040"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS5061478.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(31175..31441)
                     /locus_tag="ACG8E6_001041"
     CDS             complement(31175..31441)
                     /locus_tag="ACG8E6_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFS5061479.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31920..34154
                     /locus_tag="ACG8E6_001042"
     CDS             31920..34154
                     /locus_tag="ACG8E6_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5061480.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGISVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34302..34622
                     /locus_tag="ACG8E6_001043"
     CDS             34302..34622
                     /locus_tag="ACG8E6_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS5061481.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9817 a         7500 c         6065 g        11351 t
ORIGIN      
        1 tttatttgtg gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt
       61 ttttatttcg aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg
      121 ctaagctttt cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg
      181 gccaaaagtg aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt
      241 acgagtacat cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt
      301 tcgatattca tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag
      361 tttgaaacaa gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg
      421 tggatatctt tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct
      481 tctactacta aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa
      541 cactctacga ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct
      601 acttgtgcat tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata
      661 tcactaaaac cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc
      721 gtcagacttt gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg
      781 gaagcttctg ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg
      841 tagttggcca actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta
      901 attacgctca taagttcctc caccctttta atgatagtct atgtaagtat accatatcag
      961 acccttgtta acggtcgtgt actggataaa aaaacaaaaa aacaatccgc acaataggat
     1021 atcttactcc atcaatccca caaattctat tgtccatctg tgggattgtg gaatatctgt
     1081 tctctattgt ttggattgtt ttttatttag tgctttttta taggcagctt atctatttgt
     1141 tttctctgct gtaatttttg acttctgctt ccatagcatc aataaagata ttcaaatctt
     1201 ctacagctgt ttctttactt ccataacgac gtacgttcac tgttgcatct tccaattcct
     1261 tgtctccaac aaccaattgg taagggatct tttgtgtttg agaggcacgt atcttatagc
     1321 ccattttttc attacgatca tcaacttcta cacgcaaccc cttcatttgc aaacgttctt
     1381 tgatttcata agcataatcg ccatgtgcat caacagaaac agggatgatt gttgcttgta
     1441 ttggggccaa ccatgttgga aaagcgcctt tgtatacttc tgttaaataa gcaacaaaac
     1501 gttccattgt agaaacgatt ccgcggtgga taactactgg acgatgcgta ttttccccgt
     1561 cttctccaac atatgtcaaa tcaaagcgct ctggcaataa gaagtctaat tgtatagttg
     1621 ataatgtttc ttctgtacca agagctgttt tgacttgaac atctagtttt ggtccgtaaa
     1681 aggctgcctc gccttctgct tcaaagtaat ctagttctaa ttcatccata gcggctttca
     1741 gcattgcttg ggctttttcc cacattgcgt catcatcaaa atatttatct gtattgtttg
     1801 gatcgcgata gctcaagcgg aaacgataat ctgtaatatt gaagtcagca tatactgcca
     1861 ccatcaattc aagcgtacgt ttaaattcat ctttgatttg atcaggacga acaaatgtat
     1921 gaccatcatt caacgtcatt tctcgtacac gttgtaaacc tgacaaagca cctgattttt
     1981 catagcgatg catcattcct aattcagcga tacgaattgg taattcacga taagaatgga
     2041 tcgtgttttt gtagaccatc atgtgatgag ggcagttcat tggacgaaga acaagcattt
     2101 cgccatctcc catatccatt ggcgggaaca tgtcttcttg ataatgatcc cagtgtccag
     2161 atgttttgta caactcaaca tctcccatta ttggtgtata tacatgttgg taacctagac
     2221 taacttcttt gtctacgata taacgttcga tcgtgcgtcg gattgttgcc ccttttggaa
     2281 gccagaatgg caaaccagaa cctacttctg gggaaaccat gaacaagtca agttcttttc
     2341 ctagtttacg atggtctctt tcttttgctt cttcacggag acggatatat tctttcaaag
     2401 ctttcttgtc aaaaaaggct gtcccgtata cacgttgcat catttgattg tttgagtttc
     2461 cacgccaata agcacctgcc actgataata atttaaatac ttgtatacgg ccagtggatg
     2521 gaacatgtgg tccacgacac aaatcgatga attcgccttg ttgataagca gtaatcactt
     2581 cttcttctgg caattcacta atcagttcta ctttgtacgg atcacttgca aaaatttcta
     2641 atgcttcctg ttttgaaaga acacggcgtt cgattggcag attttctttg acgattttca
     2701 tcatttctgc ttcgattgcc ggcaaatctt ctgcagttac ttggttttgt ccattatctg
     2761 tatcgtagta aaaccctgaa tcgatagctg gtccaacgcc aaagtggata tttggataga
     2821 ggcgacgcat ggcttgggcc atcaaatgag ctgctgaatg acggactaag ccaagtgcat
     2881 cttcatggtc tggtgtgatg atctcaatac ttgcatcttc ctcgattgga cgagtcaaat
     2941 caacgagttt tccgttcact ttaccagcta aagctttttt tgctaagctt tttgaaatgc
     3001 tttcagcaat tgccaatgtt gttgttccag actcaaattc tttgactgca ccatctggaa
     3061 aagtaatttt tatcataata gtttcctcct tgaaatgttt tgattgatca gatcatcaag
     3121 ctgaaaaaca aaaaaatcct agcatctctt gaaaagagat actaggacga aaagttcgtg
     3181 gttccaccta atgttcaaac gcaaatgcgt tcctcaagcg ttgtaacggt ccgaccgccc
     3241 ttgcttcaac aagggtttcg aaagtggtca tcctttggtt ttctcctgga aaagtttcag
     3301 cctagctttt cctctcttat cgaagactct caaattcagt tggctttctc atcaatcaat
     3361 tatgtgttta tttaaccact aataaataga aaaatcaagt gggaaactaa acttattttt
     3421 tccattcccc atttacaccg tatgttcctt ttttcatctc attgatgatg acatggatgt
     3481 gttcttttgg tgcctgcgtg tttttatgaa cagcttccgt gatatctttc atcatatttt
     3541 ctaattgttc cggactgcga ccttctacta attcgacatg tacgaatggc ataatgctct
     3601 ctcctctttc tgatcgttct tttttcaact cgattctatc atactatgtt tgtcttgggt
     3661 actgaatcag gaaaaatggc tggatctttt tttgctctca aggcactcaa aatagagacc
     3721 gtatccacga cttcttgcaa aacagctccg aataatgcgg ggattagtcc cgtacttgca
     3781 attaacatca aaaaaataca aataaagatg ccaatcagta cagactgttt agctattctc
     3841 atcgtatctc ttgaaatcac tactgctttt gctacacgca agagatcatc tttcaagatg
     3901 accgcatctg cactttcact agcagctgta gaaccatgag cccccattgc aataccgata
     3961 tctgcagcag caagtgctgg agcgtcattc acgccgtctc ctaccatcgc aacaggtctt
     4021 tgatctttag ggatactttc taaaatggtc agtttatctt gaggcaagct atgggcatgt
     4081 acagaatcta attggacttt ttctgcaatc gtttgagcaa tggcagatcc atccccggtg
     4141 atcatcatca aatttttcac atcaagtttt cttagctcct tgattgtctt tgctgcttct
     4201 gggcgaatct gatccgaaaa taggatcgtt cctgcaaaag atcgatcaat tgttacatag
     4261 attgctgtat tggacgtttc tatttcttca gctgaaacaa aagaggcacg tccaatcttt
     4321 acttcatgat tattgactac tgcttggatt ccttgaccgg tcacttcttt cagttgacta
     4381 acaggataca gttggctgtt tttcacgttg ttcactaacg aacgagctaa gatatggcta
     4441 gattcctgtt ctgcactagc tgcatacaga agtagctctt ctttttttat tccattgact
     4501 ggctgaatcg cctctacttc caatatccct ttggtaagcg ttcctgtttt atcaaaagca
     4561 atcgtttttg tacgtgcgag cttttcaatc gttgttccag tttttacaac gatcccgttt
     4621 ttacttgaac ggctcattcc tgcaactaaa gcaatgggcg cagctagaat cagtggacaa
     4681 ggtgatgcca ctaccagtac ctcagcaaat cgtaaagggt cttttgagaa ccaccaagaa
     4741 ataccaccga tcaaataagc aatcaacgtg aaagggactg cataacgatc agctaaacgg
     4801 acaaatcggg caggcttttc ttttgattct tcgactagtt taataatcat ttgatactgg
     4861 ctgtctgcgg cacgtttttc agcgatcatt tggatcgatc cgtctccatt taaagaccct
     4921 gacataatct gatctccagg tttcttggtt tccgggcggg actcacctgt caaagaagat
     4981 tcatcgaccg aagattctcc tttggtcact cgaccatctg cgggtacaag ttcgccaggc
     5041 ttgactagta actgatcatc aatatttatt tcatcgatta ctatatcctg caattttcct
     5101 tctttgaatc gatgggcaat tcttggggtg ttatctaaca aagactgcag ttctttaccc
     5161 gctcgtttat tcgcgtaatc ctctagacta tctccgcccg taagcatgat caatatcatc
     5221 aaactagccc agtattcttt gacggctaat gttgcaagga ttgcggtaat cgctaataca
     5281 tctacgccat atcttcctga acgtaacgtc ttgatcattt ccaaaaacat caagaaggcg
     5341 agaatccctc cagtaaatgc gacaatcgca aatgcaaggg ccggctggtg aaaaacaaat
     5401 tcacttaata aagctatccc accaataagc aaagtaagta aaaatttttt gaaatgtgtc
     5461 attgtttttt ctcctctttt atgtaactta gagaggttag taataaattt ccagctgcct
     5521 ctttcatctt aagcatagtc cttgactagc actttttaaa acaataggcc ttaattgaaa
     5581 acaactatca ctatcagcgt ttccaaaatt catcagttct acaaacaaaa atattcagca
     5641 aggattactt tttcgattca tcagttaaaa agtacctttt ttcgaaaaac tatgctatac
     5701 ttttaattcg ttatttacgg cggaaggagc aaacatgcga tgaatgagaa acaactagaa
     5761 caaacgcacg tggatgaaac gatcgaacta atccagatcg aacaaaatat gctggataca
     5821 aaacaacaag aattgactca ggaaatgaat cattcgacaa aagacacagc taatcacaag
     5881 attcgagctg gttccaatga agctttttat gaatctgctg ttgaatatcg ccaacatgag
     5941 caggaattgc ttttgaaata ccatactctt gaagcacagc aaaaacgcct gcaaacgttg
     6001 tctgtcatga aagatagtcc ttattttgcc cgtatcgact ttaaagaaga ccgtgaaaca
     6061 gaaacattat atctaggtat cgcttcttta agagatctgg cagaagaaac aatcgttatt
     6121 gactggcgtg caccaattgc caatctttat tatgaaggtg agattggtcc cgcattttat
     6181 gaaactgaaa tagatagaat agatgtcgat ctgcagttga aacgacaatt taaaatccaa
     6241 gaaggtcgta ttttatctat ggtagatacg tcggaagtaa tcaatgatga ctttttgcta
     6301 gagatattgg atgaatcatc aagcaaccat atgaaaaaca tcgtctctac gatacaaaaa
     6361 gcacaaaatg caatcattcg ggacacaaca agtaaagtaa tgctgattga aggaatcgcc
     6421 ggaagtggaa agacatccgc tttgcttcaa cgtattgctt ttctgttgta tcataatcgc
     6481 aagtggctcg aggctgaaaa cgttcttcta ttttcaccca accatttgtt ttctgattac
     6541 atctcgactg tattgccttc attaggtgaa agcggcgtgc ctacacagac attcaaaaat
     6601 tatttggtgc agctgctgcc agagttttct ttactagatg aacagcaaca agaaagtggt
     6661 tttttgctag gggaaaaaga tccgatacaa gtgatgaaaa gcggattgac cttagtcgat
     6721 cagattgatc gctatatcca aggcatcaca aactacggtc ccttgttccg tgatatgaaa
     6781 ataaatggcc gtacgatcct atcaaaggaa tcgattcgcc aatggtataa agaaacaaat
     6841 gaacaattgc ctttacatca tagactctct ctattacaaa cgaagctcct gaaaaaatta
     6901 ggcggtctgc aaaaagacga aacacgtcag caatgggtca aagatctggc ggaagaacag
     6961 ctccaagaac tatacgcaac agatccaaat cttgaatata cagaacaaaa ggagcgttca
     7021 ttacgtaaaa aactggccaa ccaaatcgta cgtaaaagat tccgtcgaat ccatcgaggg
     7081 atcatgaatt atcaatttgt caatataacg aaacaatatc tccactttct acaaactgtg
     7141 ccactttctc ttctaacacg acataaaatc agcgagacca gatggcagga acatttgaca
     7201 actgttcgta cacacttgcg tcaaagagaa ttgcgtcaag aggatgctgt actattcttt
     7261 ttactgatgc gccgaacata tccagtgacg gttacacaca aagctcgcta tatctttatt
     7321 gatgaaatgc aggatttcgc accatcacaa gtggcattgc tgcaagcctt gttcccaaaa
     7381 gcaaatttga ctttctgtgg agatctaaat caaaatgtct ttggaaatga aacgatcgtt
     7441 ggatcattgg aagacctttt cgtggaccgt gaagtcactc gtttccaatt aacgacaagc
     7501 tatcgctcca caaaagaaat cactgatttt gctaatcatt ttattgtgaa agaacatcaa
     7561 gtagaaacaa cagcaagaaa aggagacctt cctttgctca tccaaggaac aacagatgat
     7621 atcaaattga attggttagc agataccatt cgaacgactc agggaaaagc ccggtattgg
     7681 cgtacagcaa tcatagctaa aacacaagaa gaatgcgaag ctctttatgc tcaactgcca
     7741 gacgagttga aacaacaagt ccagctgatt ttaaatgaga gtgactttat gaaacgttcc
     7801 attattttac ttccagcctt tttagcaaaa ggtctggagt ttgaccgagt tttcttgtgg
     7861 gatgtagatg cagaacattt ttcttctcag caagacaaat tgatccttta caccatgtgt
     7921 acacgtgcga tgcatgaatt gacccttttt acctccaata atgaaagtcc tttgttaaac
     7981 aatatggata tttcatctta tgagaaaaaa gtactgtaag taaaaagaca actgctatca
     8041 ttaatgttag cagttgtctt tttatttaca ataataattg cagaaaaagt aattcaatca
     8101 tttaaggtca tttattctat atataaatcc aaatctgttt caggatattt taatttgaca
     8161 agcgaaataa tcattttcag ttcttccatg ttttctggat attctgggtc aaataccttg
     8221 ctatgacggc caatcccgcc attgtcgatc aatcggtaaa ttcgattcca ttcaatgaat
     8281 tggtcaattg attggaaatt gctgattgga tagccgccac aatccaaaat ctctgataca
     8341 cgaaatgcta ggtatgcact atcgatcaca ttattttttt tcgtgtctat cttctcattt
     8401 ctttcgataa gaattttttc aggacgatgc ttcaagctcc actctaatag tagaaagaaa
     8461 gcctccatcc gatactcttc ttcaaataac cgaaaggctg aatcagaacg acgaaaaagc
     8521 agcggaatca gataattttg tctataaatc agcattggtg cgcggtccac catacactct
     8581 aaataacggt tcagataccc tgtaccgaca tagtttccta atcgatagcc ttcaacgccg
     8641 aatcttgcgt atttttgcgc taattttgaa caatcagtta cgttcacagc taactcttct
     8701 actttgcggt aaccgcttaa gatacgttca aaaggttcta aagtgatttt tgcttccata
     8761 ttcttccccg tccatcttcc acatacttca tatcatttca catgatactt atgctctcat
     8821 cataccacag gttgaattag aaaagaaaat aaatcacaca aaaaagcaag acaatctgtt
     8881 agtgagattg tcttgctttt cgaaaaagac tgaatacttt tcgttaaaac tggttgctta
     8941 gtcttctaat gccacattat ggaacacatg ctgtacatct tctagatctt ccagtgcatc
     9001 aatcattttt tcaaatgttt cttgatcttc tggagatagt gttacctcgt tttgagggat
     9061 catttggatc tcagcgattg aaaaatcagt gattcctttt tcttttaatg cttcttgtat
     9121 actatggaaa tccgctactt caccgtaaac gatgatttgt ccttcttctt ccattacatc
     9181 gcgaacatcg atgtcttttt ccattaggta ttcaaggatc tcgtcagcgt cttctcctgc
     9241 aaaagcaaag attgctgtat tgtcaaacat gtaagaaacc gctccggaaa cacccatgtt
     9301 cccgccattt ttcccaaatg ctgcccgtac gtctgctgct gttcgattca cattgttcgt
     9361 caatgtatca acaatcacca ttgagccgct tggtccaaat ccttcatagc gcaattcaga
     9421 gtatgtttca tctcctgcac cttttgcttt ttcgattgct cggtcaataa tatgttttgg
     9481 tacattataa gttttagcgc gatcaatgac gaaacgtagt ttttggtttg catgcggatc
     9541 aggatcacct gattttgctg ccgcgtagat ttcaatccca aattttgcat aaatccggct
     9601 attattagca tctttagcag ttttctttgc tacgatattt gcccatttac gtcccatatt
     9661 ttcacttcgc tttctgattt gatagtttca taacgcaaaa acgtcctgtt ccattatact
     9721 tgtgaatcac gattctgtga agcttttttt ctttcttcct tcattcttct catttttagt
     9781 caattatcta tactcggatt gggatttgcc cgaaattctg ttccggaata ttgcctccca
     9841 attgatagat ttgatcactt ctaactaaca gttccgcttc ttgtgcctct ttttttccga
     9901 ttcgggaata aatagaaatc tctttttttt cttttaagaa tcttcttcca acatcggtaa
     9961 atccaagtag tcgaagataa tttttatttt gttggacagg aatctcttgc tttttgatat
    10021 tcagcaagac ataacaagat aatcgttgga tccgtgtcca tgtatatctt tttgttttta
    10081 cctttgacac aaaatcctca aaactatcag cagttgcagc tgctgttttc atgcgcgatt
    10141 ctattccttc ggtcatctga tagatatctg tcagttccag caaagtagaa gataacaacc
    10201 gataacgcaa atacggccaa taagattccc aagtctgcag cgaataagtt gaaagatctt
    10261 cttctgtctg aggcggcaca agtccagcta ttctttttcc ttcttctaac gcatggcgaa
    10321 tggctgttgc actggctatc ctctgatctg tgatgatttt ttcatgatag cctgcacttt
    10381 tacgtcggat cggatgtaat tccatagggt ggacatactt ggcattttcc tttgcataac
    10441 ttaatcccaa gatatgattt ggcgaagaaa aatcaagcgt aatatcggga tacagactgc
    10501 gaaaaacctc agtcattttt tgcggataac tatacgtttg atcagacagt tcttgaaatg
    10561 cctcttctat cctatcttga ttctcttgga caaagcgtcc gaaactgctg taatcaaacg
    10621 aagtctcttc ttctgttcca aaacacaaac tgtcacagtc cagtgtttgc aagattttta
    10681 ctgcaccttt agcaaaatag tcagctgact gaaccgccca ttcgacggga agctccacta
    10741 ctaaatccac accattttgt aaagcttcat tcgcgcgatg ccatttatcg atgatagccg
    10801 gttcgcctcg ttgcaaaaaa ttcccgctca ttacagcaat gacgacctct gctcctgttg
    10861 tttttcgtgc catttccaca tgatagcgat gtccattgtg aaaaggattg tactctacga
    10921 taatcccgca gcttttcatg cgcatcctcc ttcatagtta tcgataaaaa agaaatagag
    10981 acaagtaaaa aaaggcaaaa atcggaaaaa atacccgaac tatataaact attctcgcaa
    11041 acacaatact tctgcgacga aaagctgcac tgcaagagca atgcctatat cgttcggata
    11101 tataatttat gttgaagatt tattcttttc ttgttaactc gagcaggcaa aaccgctagt
    11161 ctttgtatgc tacaaagaac cagcgctttg atttctctga tgctgcttcg tctgtaaaat
    11221 cagaaaatgc ttcgactttc gaaaagccgc tgttttccag cattcttaaa taactttcca
    11281 atggataggt acgctcttga tgtagttcat cttcacgaat aaatcgacca gacccttctt
    11341 gttcttcaac gaaaaaggta agaaaatgtt cgatgctgtg tttctcgctt cctggataac
    11401 tatcccacaa aaaagcaaaa ttatcccctt gataatgata gctatattct ggaaaaattt
    11461 catctacttg ataggtggag tgtacgtcaa ataaaaatcg gccattttct tctaacgctt
    11521 gatagacttc atcaaacact tgctgtacct cttgtctatt tttcatatag cacagcgaat
    11581 cagaaaaaca ggtaattgcc tgatattgtc cgatttctga taaatcgagc atattgcctt
    11641 ccacaaattg gatcgatgcg tcttcttcat atgctcgctg gctggcaatc atcaacatct
    11701 cttctgaaag gtctaaccct gtcactttat atccatcttt tgcaaaggca actgctaatg
    11761 ctccagtacc gcatgccagt tccaaaatat tttttgtgtc ttcttgtaaa tgacgtttcg
    11821 aaaaagctag ccattgctga taaagtgttt cgtccatcac ttcatcgtaa atgaaagcaa
    11881 atgtttcata agccatatgt taatccaacc attcactaag atcgactagc ggagcatctg
    11941 accatagttt ttccagatta tagaaacttc gttctggtgc atggaaaaca tgtacgatca
    12001 catctccaag atcgatcaat acccattttc cgccttcttt tccttcaata cgttttactt
    12061 catatttgtt ttcttcttct ttatcaatga tttcttctgt aattgcgttg atttgacgtt
    12121 cgctatttgc tgaacagatc atgaaataat ctgccaacaa ggaaacattt cgaatgtcta
    12181 aagcgataat gtcttctgcc cgttttgaat ctgctgcttc aaccgcaatt tttaattgtt
    12241 ctttactatc tatgatgatt tcctcctaat atgagcagct tatttttttg ctacccagta
    12301 attatatgtt tcgattgttt ttggataaat ctgttgttct tgcgcaagca aatgggccaa
    12361 tgtatgtttt gtttcaaatg ctacagcttc gtctagatca gcccatgcaa tggctcgtgc
    12421 atcttgtacc ccaggaaaat ctcgacctgg ttcaatatag tctgctacat aaatgatttt
    12481 gtcaaggaga ctcatcttag cagccccagt cgtatgcacc cggattgcat gaagaatttc
    12541 tacatcggtg attcctaatt cccgctctac aaacgaagcg ccaacaagac catgccagat
    12601 tgcattatta taattcaata actctggatc aaaaccgtct cttacgatta ccattttgaa
    12661 ctcctcatct ggtcgttctt ttgcataatc atgtgtcaat gcagcaatac tagctttttc
    12721 aggagaagca ccgtattttt tggcaagagc cacggctgtt tcttctactc caagcacatg
    12781 cttgaagcgt tgttcgctca ttctcatctg tattttttgc atcaggtttt ctctagaata
    12841 agcagtatat tcattactat agtccatatt catacaaccc ttcattttga atataatcaa
    12901 ttactttatc tggaacaagg tagcggatag aacagccttc tttgatcttt tgacgtattt
    12961 tcgtggaact gatgtcgatc tcaggtacat ctacccaaat tacaggatat ggagtatccg
    13021 ttgtgtatcc tggtcgacgt atacccacaa agttgaccat agaagtcagc tcatcgattt
    13081 tataccattt tggcaaatat tctaccatat ccccaccgat aataaagtaa taatctgtat
    13141 ccggattgtt ttgcgtcaat tccttcatag tatcataggt gtaacttttc ccaccacgag
    13201 ctaattcaat cgtttcaatc tgcaaaaaag gattgtcttc cacagctaat tccaacatgt
    13261 tgagtcgatg ttttgcatca atcgtctgtt tctcatcaac atgaggtggc tgataagaag
    13321 gcatcaaaaa tacgcggtca agacctaaat tccggccagc ttgttcagcc atcactaaat
    13381 gtgcaaggtg aacgggattg aatgttccgc ccaagatccc tacttgcttt ttcttctcta
    13441 agaattgagg catttcttca ggaaataatt gtgaacgaac aaatgtctta gcttgcgtat
    13501 tcatcatggt cactccttat attgctttta cttcttttga tagcctttga tacttttctt
    13561 ttgtggaagg tttgtataat acaataacac gtccaatgat ttggacgatt tcgcaatcta
    13621 tttctttttc taatacttga gctgcttctt ctgcaatctc atctgtattt tgaagtaacg
    13681 atactttgat cagttctctt ttttctaaag cttcttcaat ttgaacgatc attgctgaat
    13741 tgatcccacc ttttccgatt tgaaaaatcg gctgaagatg atgcgctttg ctacgtaaaa
    13801 aacgcttttg ttttcctctt aaactcataa ttttccttct tccttaaatc aatgctttgc
    13861 gtttcaatac atctacaccc tttggcgccc agcccgcaat tactactggc gctgttacag
    13921 tgatccagcc tagaccggca aaaacgatgt ctgttttttc tttcacagaa aactcaaaac
    13981 gaacaagctc cggaaatgcc tcgacttcat caggacgagg aggctgtaat aatccaccta
    14041 catgtttttg ataaaattcg tctgcttttt ccagtttcgt tcgatgaata tttacatcat
    14101 ttgacacata agcaataaat gaagcacgat tgccgctgat aaaatcaaat cttgcaagtc
    14161 cgcccaagaa aagtgtttgc ccttcattca gctgataaac tttaggtttg atttctttta
    14221 acggtgcaat gatccgcaaa tcttttttac ctaagtagtg cgccatttga tggcgatgga
    14281 taattcccgg tgtatcaatc aaaaaatgcc cgtcatctaa aggaatctcg attttatcca
    14341 aggtcgttcc tggaaactgc gaagttgtga ttacatcttg tactcccgct gtatttttga
    14401 tgatttgatt gataagcgtc gattttccga catttgttac tccgacgata tagacatccc
    14461 gtccatcgcg gtattcttcg attttttcta atagttccgt catctcatga gatttcttag
    14521 cactagttaa taatacatct actggacgaa ttcctgcttc gtaagcacgt tccttcatcc
    14581 attgtgttaa tttagttttt ttcaatgatt taggtaatat atccactttg ttcccaacta
    14641 aaagtactgg attgtcaccg acaaaacggt gtagtcctgg gattagtgaa ccattgaagt
    14701 caaaaatatc caccacattg acgatcaatg catctttctg ccctaattca ttcagtaagc
    14761 gcaaaaagtc gtcatccgtc aatgagacat cttggagctc attataatgg cgtaatctga
    14821 aacaacgctg acaatagact tccccggaag ctaaaccttt ttccaaagcc gtttgcggcg
    14881 tgtatcctaa ttcaccgggt ttgtttgttt gtataacggc accacagccg atacattgta
    14941 attcttctgt cattcgattc cacctcgcca gcccatatca gggtgttttg ctaatagata
    15001 ggccataatt cttttttccc aaaaacgatt aaattgtgtc ttccaactat ctgttgcgat
    15061 aatgggtttt acaaggatgc tacgtatccc tgcacgattg gctccacgta tatcagtcat
    15121 aatctgatcg cctaccatta ctatctcatc atctgctaaa tccaattgtt tctttgctat
    15181 gttgatccct cttgctaaag gtttcattgc tcgtgcaaca tacaaaagat caaatttttc
    15241 aatcgctcgc gcgacacgtt ttgaattatt gtttgaaaca acaacaacag ggattcctgc
    15301 atttttcatt tctaaaatcc aagtaagcaa ctcttctgtt ccatctggat tattccatgc
    15361 aatcaacgta ttgtctaaat cggtcaatac tgctttgatg cctagattct ttaattgggc
    15421 aggtgtaatc tgatagatgg cttctaccat ccatgttggt ttgtaatttg aaaacatgct
    15481 gtctcctcat ctgcaaaaaa tgaaggagca cataaagtac tcctcaaccc cgtattatac
    15541 tgcatatcag caaaaaaaac tacctctata ctcgttcgtc tttcagaaaa agcatttctt
    15601 tacaaaatga ccaaaaataa agattgatca cagaaaaatt tagaaacaaa aaacaactaa
    15661 gcgagcaaga ccattcttta ctctttgctt agttgttaaa cacctaatat tggcggaacc
    15721 tcttgtatcg tttttccagt aattcatgct ggtcgaatcg aacaagttcc gtcaatcttt
    15781 caatcaacgc ttttttcata atgtgtgcga ctttatatcg tggcaactgc tcgccgttga
    15841 acgtttcagg aataactcga tcaacgattc ctaactcgcg tagttctggc gcagtaattt
    15901 tcatcaactc agctgcttct gcggcgcgac taccgtcttt ccacaaaatc gacgcaaatc
    15961 cttctggaga aagcacagca taaatcgttt cttccagcat ccagacttca tccgctaaac
    16021 ctagagcaag ggcaccgcca cttccacctt caccaatgat gatggcgatg atcggtacct
    16081 ttaaatcaga catttcgagc agatttctgg caatagcctc tccttcgccg cgctcttctg
    16141 ctcctacacc gcaatatgca cctgctgtat tgatcaatgt aatcaccggt ctattgaact
    16201 tttccgcttg tttcatcagt cgtaaagctt ttcgatagcc ttcaggatgg ggagaaccaa
    16261 aatttctgcg gatattttcc tgcagatccc gtcctttttg aataccgatc actgttaccg
    16321 gtttgccatc taatcgagcg attccaccaa caatcgcttc gtcatctgca aacaaacgat
    16381 caccatggca ttcctcgaaa gaatcgaaga tctcttcaaa aaaatccaag cttgtccatc
    16441 gttctttagc tcttgctaac tgaacgacat catgtgctgt cttatccacg atctatccat
    16501 cctttctgtg tatgcaattc aagaagcgta tggattttct gcttcaattc catgcgggga
    16561 acaatctgat cgacaaaacc atgagataat aagaattcag ccttttggaa atcttctgga
    16621 agttcctgct tgatcgtctg ttcgatgacg cgtcttccag caaaaccgat caagctttgg
    16681 ggttctgcta gaatgatatc gccttccatt gcaaaactgg cagttactcc tccggtcgtc
    16741 gggtccgtta aaacagttag ataaaaaaga ccttcattgc tatgccgttt gacagcagca
    16801 gagatttttg ccatctgcat tagagaaaag attccttctt gcatccgtgc gccacctgaa
    16861 gctgtgaaca agacgactgg aagactttcc tttgttgctc gttcgaataa tctggtgatc
    16921 ttttctccga caatcgttcc catacttccc atgataaaat taggatccat cactccaata
    16981 atgaagggaa tcccttgaat ggttgcttta ccagtgagga ctgcttcatg aagtcctgtt
    17041 ttatcttgca tttttttaat tttttccgta tattcgggaa atgctagcgg atctttggtt
    17101 acaagatccg tatcccattc ttcaaaactt ttttcatcaa taatcaaagc tagacgctgc
    17161 catgcaccga ttcgaaaagc gtatccgcaa tgagggcata ctttttcttc accgatgtct
    17221 ttcgtataaa ggatatgttt gcaatttgga cactttgccc acatgttatc aggaacagat
    17281 ggcgagttag caggtttgct ctctctatta ggattgatcc gaatatattt ctttttctta
    17341 aacagagcca tctatgcgcc tcctatctcc gtttctggtg tccaattcgg taaaaattct
    17401 tcttgtaaaa atgctgtgct gtaatcacct gctatgactg cagggtgact aatcaaatcc
    17461 atctgaaatt ctgcgttcgt gatgattcct tctgtgataa attcacttaa ggctcgctgc
    17521 atcttcatta atgcttcaaa acgcgtattt ccatggacga ttatttttgc aatcattgaa
    17581 tcataatatg gcgggatcgt atatcccggg taaactgcac tgtctacccg cagtcccatc
    17641 ccacctgctg gaagcagtaa gttttggatc ttccctggag aaggagcaaa atggaaagca
    17701 ggattttctg cattaatccg gcattcgatt gcgtgtcctt ggaatactac ttcttcttga
    17761 cacacattca aaggctcacc ggctgctatc tccacctgtt tttttactaa atcgattcct
    17821 gtgaccattt ctgttactgg atgttccact tggattcgag tattcatttc catgaaataa
    17881 aaatcgcctt cttcatccat cagaaactcg atcgttccgg cattttcata attgactgct
    17941 ttagctgctc gaacagcagc gtctcctagt gcttctcttt ttgtttgtga gatcactaca
    18001 gaaggagatt cttccagtac tttttgatta ttacgttgca aagaacaatc tcgttctcct
    18061 aaatgaatga cattgccaaa atgatcacct aaaatctgaa cttctatatg gcgagcagga
    18121 taaatgattt tttccaaata catatcatca tttccaaatg ccgctgctgc ttcttgctga
    18181 gctgaaaaaa aatgctgtgg gagctcttct tttgattgaa ctttccgaat gcctttcccg
    18241 ccaccacctg cagctgcttt cagcatcact ggataaccaa ttctgtctgc tatttccaat
    18301 gcttcttcta ctgtagataa tacaccagta cttcctggaa tgactgggac atttgcttct
    18361 tgcatcaatt gacgcgcatt gatcttattt cccattgcat cgatcgttgc acttttagga
    18421 ccaataaatg tgatattgca ctcttcacac atggaggcaa atcggctatt ttcagataaa
    18481 aaaccaaatc ctggatgaat tgcttcggct tttgtcacga ttgcagcgct cagtacttcc
    18541 tgaacattca gatatgaatc aacggctttt gccgggccaa tgcagattgc ttcatctgct
    18601 aactgtgtat gaagtgcttc ggcatctgct tctgaatata tcgctacggt ttggatccct
    18661 agttcgcggc atgcacgaat gatacgtaca gcgatttcac cgcggttagc tatcaatact
    18721 ttagaaaaca tatttcacct atccaatcat aaaggttaat tccgcttcgg ctactttttt
    18781 accgtctact ttagcgattc ctttgcctat tccggctgaa gctttcactt tcaatatctc
    18841 aacttcaagg tataaggtat ctcccggtgt aactttctta cggaattttg ctttgtccaa
    18901 tccgccgaaa taagccgtct ttcccttgaa ttgttcaaga gaaagtaatg caactgctcc
    18961 tgcttgagcc atcgcttcaa caattaatac ccctggcata acaggttctt ctggaaaatg
    19021 tccttgaaaa aagggctcat tgatcgttac atttttctta gcaacgatcc gttctccttc
    19081 aaccatttct tccactcgat caatcaaaag cattggataa cgatgtggaa tgatttcttt
    19141 tatttcttgt atgttcattg atttccctct ttcctgcttt gcttattttt gctgaatcct
    19201 aaagagtggc tgtccatatt cgacaactga ctcgttttct acaaggattt ctgtgatcac
    19261 tccgtcggcc tcactagtta tctcattcat cagtttcatc gcttcaataa tgcagataac
    19321 ttctcctttg gcaacttgat cacctacttt tttgaaagct ggtttgtcgg gcgccggttg
    19381 cagataaacg attcccacga ttggcgaaac aacctcgatt ccatcaaata ctgccgattc
    19441 tttttctttc attgtttctg gagcagttgt tgcttgttca ggtatgacat tgactggctc
    19501 cgaaactggc aacgtttcgt ttgtttgaac catattcatg cctcgacttg ttttattttt
    19561 attcatatac aattcaaatt gaccttcacg caaatcgaat tccgttagcg aggattgatc
    19621 aaattgactg actaattctc tgatttcatt gatatccaaa attattcctc ccagcgtttc
    19681 atgcacagaa ccgcattatg tccgccaaac cctaatgaat tactcattgc ataagtaaaa
    19741 cttgcaggtt tgctttcgtt tttgacaact gtcaagtcaa tctcaggatc ttgtttttct
    19801 acattgatcg taggtgggat aaattgatgt tctaatgcca gcaaagttgc caaggcttcg
    19861 atcccgccgg ctgctcccaa taaatgtcca gtcatcgatt tcgtgctgct gacgtaagta
    19921 tctctgaaat tttcacctaa tgcgatttgg attgcttttg cttctgcctg gtcattggct
    19981 ggtgtacttg ttccgtgagc attgatatat ccgacttgtt ccggctgtac accagcttct
    20041 tccatcgcaa gcttcattgc tttagacgca ccagaaccat ctggagtagg agctgtcata
    20101 tggtaagcgt cacagtttgc accgtatcca acgatttctc catagatttt ggcaccgcga
    20161 gcttgtgcat gttctaaaga ttcaagaact agaacacctg ctccttcacc catcacaaaa
    20221 ccattccgat tttcgtcaaa cggaatcgaa ccacgattag gatcagttga ttctgagagt
    20281 gctgttaaag ctgcaaatcc agcaatcccc atctcacaaa tcgttgcttc tgttcctcca
    20341 gcaagaatga catcagaata gccatgtttg atgttacgga atgcttcccc aattgaattc
    20401 gttccgctag cacaagctgt tacgatagct gtacaaatac cttttgcacc tacgcgcaaa
    20461 gcaatatttc cagctgccat gttggcaatc gccattggta cgaacattgg agaaactcgt
    20521 tttgcacctt tttcatgtag acgaataact tggttttcga ttgttggaag accaccgatc
    20581 ccagatccga tgatgacgcc aaaccggtct tgattcattt tttctttatc taaaccgctc
    20641 atctccattg cttctaaagc agcgtagatt ccatagatcg aaaacatatc cattcgtttg
    20701 ctatcttttt taataaaata tttatcgtaa gggaaatctt tgacttcacc cgctaaggta
    20761 atccctgttt ctgaagcatc aaatttagtg atcggtgcga tgccattcgt cccctctttt
    20821 aagctattca aaaagctgtc tgcgtcattt ccaataggtg aagtgactcc atagccagta
    20881 attaccactc gattcatttg tttgctctcc ttttcttatc cgtgcatgac caatccgcca
    20941 tcgacattga tgacttgtcc ggtgatatat ggattttgtg ctaagaaaac tgctgtttgt
    21001 gcaatatctt ctacttgtcc gaaacgctgc atcggaattt gcttttccgc ttgttccttg
    21061 actttgtctg ctaaaacttc cgtcatatca gttgtgatga atcctggcgc aatggcatta
    21121 catgtaatgc ctctagttgc agcttctcgt gccacagatt ttgttaatcc tactacgcca
    21181 gctttactag ctgcataatt tgcctgacca atatttccaa tcaacccaga tacactagag
    21241 agattaatga tggccccttc tcgctgcttc atcatttttt tcaatacgtg ctgcgtcata
    21301 ttgaaggttc caatcaaatt gatatcaaga cattttttga aatcttctgc atccattcgc
    21361 atcacaagtt tgtcatttgt gattccggca ttgtttacta acacatggat cgatccaagc
    21421 ttttcttctg cttctttgat catttgtccc gctttctcat aatcagagat gtcaccggaa
    21481 actcctacgc attttacacc gaatgcttcg atttcttcta tcttttcttt tggaatctct
    21541 ccgcgaccat tcaaaatgat atttgctcca gcttttgcaa aagctaaggc catcgctttg
    21601 ccgattcctc ttgtactacc tgtgataaat acattttttc cttttacatc cataacgttc
    21661 ctccttatag tattgctatt gtttcatcaa atgtctgctt attctcaaca cgcagaactg
    21721 gtattgtttt atcgattttt ttcataaatc cgcttaatac ttttccgggg ccgacttcga
    21781 tgacttgttc gatgccgatt gtttttaact tatggatact ctcatagaaa cgaacaggtt
    21841 gcatgacttg ctgttcaagt agtcccgcga ttgtttcttt cttcattatt tccgttgttg
    21901 tgttgcttat gataggaaaa gaaggttcag agaattgaat ctgacttagg tccttcgcca
    21961 gtttttttgc tgccggttct aaaatagctg tatgaaaagg cccgctgaca tttagaggga
    22021 tcattctttt gaaccctttt tcttttaata gtaaaacagc ttcgtctact gctttttctt
    22081 cgccaccaat cacgatctgc tgaggtgtat tataatttgc aggagatacg atgccgtact
    22141 tgctagcttc atgacagctt tcctcgatcg tttcaatcgg cgcattcatc actgcaacca
    22201 ttttcccgct tccagctggg gcagcttctg tcatataagc gcctcttttt gctactaacg
    22261 caactgcttc agtgaaggat agcgcattac tagctactaa tgccgaatat tctcctaaac
    22321 tcagtcctaa agctgcatct gggatgattc catgctcctt caataagcga taatacgcga
    22381 tgctgactgt tagaatagct ggctgtgtat attgcgtctg atccaaacgt tcattttctg
    22441 taaagcaaag ttcagccata tcgtagccga gaatctcact tgcttcatca aatgtctgct
    22501 ttacaacggc ttcttcatat aaatcttttc ccataccttg atattgtgca ccttggccac
    22561 taaataagaa tgctgttttc atgtttttct ccaatatatc cagaatatat gtttgattct
    22621 tcctattcaa aaaggccgag acattcctat ttcaggaaat gtcccagccc ccaacctaat
    22681 tttaatgatt atgcagtttg tttttctacg taagtcacta aatcttgaac cgttttgatt
    22741 ccgtcttctg tttcgatttt cacgtcaaat tcatcttcaa tttcattgat gatttggaat
    22801 aaatccaagc tgtcagcttc taagtcttct tggatgtcag tagtcaattt gatttcttca
    22861 gtttcttttc ctaattcttc tgcgatgatt tcttgaattt tttcgaatac cataataata
    22921 tattcctccg atattgtttg ctttttattt ttataattta acagtctaca actccatgag
    22981 aatacttccc catgttaaac ctccgccata gcctgtaaat attactttct gctgttttcc
    23041 caaggtgatc tttccactag aaactgcttc atccaataag atagggatgc ttgcagcaga
    23101 tgtattccca tatttatcca tattggtcag aaacttttct tgcggcatct ttacttttcg
    23161 ggcaattttc tcaataattc tgaggtttgc ctgatgcaaa agcagataat caactggttt
    23221 gtctttcatg acttcttgga tattgtttgc aacgtctcgt acggcaaaat caaaaatatc
    23281 tctgccagtc atttgcaaat aatacgaacc ttctgaatct tctgaataga acggattttt
    23341 attttgctgg tagccagcgg tcaatgagct acttctggta ccgtcagctg ctaatttttc
    23401 tttcagcaat tttttctgtg gattcgcttc taagagaatc ccacctgctc catcgccaaa
    23461 caacacagca gtcgtacgat cagaccaatc gattagctta gatagtgttt caccaccaat
    23521 cactagtccc ttagttgagc cgcagcggat cattttttca gccatgctta acgcataaac
    23581 aaatcctgag cacgcagcac tgatatcaaa agcgatagca tgagttgctc ccaatttccc
    23641 ttgtacttgt gctgctacag atggtgtcgt gtaatcgggc gacattgttg ctactaaaat
    23701 aaaatccaaa tctttagggt ttagattttt ctgttttatc aatttttccg ccactaaaat
    23761 gcataaatca gaagtctcct gatttgttac acatcttcgt tcacgaatac ccgtccttga
    23821 cgaaatccat tcatcagagg tatccatcat cttggaaaga tcattattag taataatctt
    23881 ttcaggaagg acgctggctg ttgcagtaat cattccaaat tgttccatta aatatcctca
    23941 cttatagtct tgcaagaagt cgtgcaagtt tttcagtgca tccagcaagg catgctgttc
    24001 ctccgtagcc attccgtcta agatgttctt gaccatttca cgatgaaaat gctgatggac
    24061 ccgaaatagt aactttcctt ttttcgttag tcccaacttt acaacgcggc ggtcatcttc
    24121 actgcggata cgttcgacat agcctttttt tactaacttg ttaatagcag tggtcaaggt
    24181 cccaacagta attgatagtt ctttggctac ttctgaagtt gtttttttct tgtacatccc
    24241 gattgcctcg atcgtgtgca tctctgtgat tgacagatct ttgaattgag attttttcag
    24301 ctcagactct tcgattgtca aaatatcatt gaagacacta acaagataat catttactgt
    24361 ttttaaatct ggttccatgt tccaccgtcc actaattagt ttgattatca aatcttttga
    24421 tgttcaaact atatcttaac ttttttcaaa atgcaacctt aaaagatcat ttttttacta
    24481 tatgagcttt ttttaagatt ttactcattt aaaataactg ataaatcagc atttataatt
    24541 tcttttatta gaaaaatcat ctatctcaca tattttgatt atcaaagtat tttctgtgaa
    24601 cagaactata gaattcctta aaacttgtcc attatttcaa aatttgttat agtcttttta
    24661 ttcaaagcgt aaaaggagat cctatgttaa aagaaaaaat gaaaactctc cccatgctgc
    24721 cgggtgtcta tcttatgaaa gaccgtgatg gtaaggtcat ctatgttggt aaagcaaaac
    24781 ggctcaaaaa tagagtaagc agctattttc atcagaataa acagcattca aaaaaagtat
    24841 tacgtatgat tcatcatata acagattttg atttcgtcgt agtagacacg gaattagatg
    24901 ctttattgct cgagtgtcaa ttgatccaac attaccgtcc tttttataac cgacaaatga
    24961 attatttcag taattataat tatgtacata ttaccaataa gggatttgta ttaacagata
    25021 ctcctacggc tcgaacatac gggccttttc gtctctataa aaaaatgccg tctattttgc
    25081 gtataatgga agaaacttat caaatgcctt ggttgtctga aattagtttg ttagctttgc
    25141 gagcccagct tcctgaccta caggaaatga cttttgaaca gaaaaaaaag gaactccaag
    25201 ggctgtttca aggccaaaat aaaaagctac taacttactt gaaaaaaaga caacaacatt
    25261 tcatctatca gttgaatttc gaaaaagccg gtatgcttca aaaagatatc gaattagtta
    25321 cttattttat tagacggatc caggaacaaa agcatttttt aagaacacca tcgctcacct
    25381 tcagcatgcc attagcagca gatgagagtc agaaaaagca ctacctcgtt tgttatggtc
    25441 aacttgcaga aacaatgatt gcctctgctg acgtttctcc tgacttctat tatgaaaaga
    25501 aagaagctca tttatctttg aaacgtcaat tgtcaaaaga agaaatcgat cctgtacaaa
    25561 tcttgatcag ttaccgcaaa aaattagaaa aagaacagtc tgaaattgaa ctgttaaaca
    25621 aaaaagaagc tgaaaagcaa ctaaattagt tgcttctcgg cttcttttag gcatcttcaa
    25681 cttcaaatgt caattatcct aaaatttatt ggattaagtc atagatttcc atcgctacga
    25741 tatcgatatt atcaaattcg tattgctgat gagtatctgt ttcagttaat tcaaatgtat
    25801 cggtcgcttt atcataagtt actgtacact tctctacgcc ttctttttca aaacggcgaa
    25861 cttgcagttc attatctgct gactcgacca tagcctccag tcgttttatg attgcgacta
    25921 attgtgaatg tgtcatcgga ggtcctcctt ttcttatttc tgttatattg tatcaaaagc
    25981 atagcctact tgcattgatt ttctctaaaa aaactcgttt tttatgattt tttttctggt
    26041 atttttcctt caggccacca aagtttgatc aaagcttggg ttccgtcatt ctcaaaacct
    26101 ttatctgcca actcttcata tagtttcaat gcttgggtag ttgctggcaa tacaaggtcc
    26161 attttttctg cttcttctaa tgcaatcttt aaatctttga taaaatgttt gacaaaaaaa
    26221 ccaggtgtgt aatcttcttt taaaatacga ggagaataat tgcttaaaga ccagttagct
    26281 gcacttccac ctgaaagcgt ctcaatcact ttctttaaat caagtccgtt tttttgtgca
    26341 tagacaagca tttccgtcat tcctgtcatc gtacctgcaa tcatgatctg attagccatt
    26401 tttgtatgct gacctttccc agcagaacca tgcaatgtaa atgtcgtacc aaattctttg
    26461 aataaaggca gtacttgatc ataaacggct tcttctccac cgaccatgat cgtcagtgta
    26521 ccattctttg ctcctaagtc accaccggat accggtgcat ctaacgcatc tgcgcctttt
    26581 tcttttgctg tttttgcaat tttttctgcc aggcttggtg tacttgtagt caagtcaact
    26641 aaaatatgtc ctgagatatc tgcagaaaaa atccccgact gtccatagta gatttcttct
    26701 acatctttgg ggtaacctac cattgtaaag agaatattac tttgttctgc taccgctttt
    26761 ggtgtatctg cccagatagc tccttctgtt actaaaggat cagcttttgc ttttgtccga
    26821 ttatagacag ctacttcgtg tcctgcttct agtagatggc gtgcgacagc acttcccatg
    26881 acgcctgttc cgataaaacc aagtttcatt ttttcgacct cctaaaaatc aaaaatcctt
    26941 gcttctatgc tagtatacgc tagaaacaag gattttttca taaaaaatgt ttgaatttta
    27001 tcttttaaat gaatttaatt ttattttgat tcgttcaatc gagtcttctt cttcttttct
    27061 tcgcatctct tcttcatgat aaacaaccat gtcttgataa aattcgatca tagcttcgcc
    27121 gaaatgtgta gatgaaatat cataaagttt agcttctaaa acttttggat ctttaggaac
    27181 ttcactggaa atataatcaa taagtgctgg tgcaaagtct tcgtcggttt caaaagtttt
    27241 ccctaatgaa ggatgatcga acaatcggtt caaataagca tttccttcca cgacacaagg
    27301 tgttccggct gccatcgctt ctgtataggt aagcccttgt gtttctgaag tagaagcact
    27361 gacaaaataa tctgccgctt tataataaag tgctacatca ttgttgtcca cttctcctgt
    27421 gaactgcaca aattcttcta actgcagttc tgagatcaat tcctctaact cttctacata
    27481 aggtcctttt cctacgataa ctaatctggc gttaggataa gcggagatga cctgaggaaa
    27541 cccattaatc aaggcttgga tatttttctc ataagaaatc ctgcttaaag aaagaatcat
    27601 caaatggttt tcctgaagac ctagtgactc ccttaatttg ctgatatcct gctgagtgat
    27661 atcagaacgt ttgaattttt caatgtcgat accagtagga atcacgcgaa gcggtgctat
    27721 tacaccatag ctacgtaatg tatcaataac acgttcactt ggacaaacta cacctgtcgt
    27781 atgatttgca aataatctag acaagtattt tacatgggat tgcctgatca cttttccttt
    27841 accgatataa tgaaggtagt cttcatacat cgtatggtaa gtatggatca gaggtatttt
    27901 caacttctta gctaccatct tcccaagtat ccctgctccg aattctgtat gggtatgaat
    27961 aagatctagt tctagttctt tggcaaccaa ataagcatac cacatgcctc taacaacaac
    28021 tcttcgatcc ttgaaagaaa taaatggtac acttggcatt cgaatcacat ctttttccaa
    28081 cttatccgca ttaggatcgg tcgttgtaaa aatatagact tcatgcccat gtctttctaa
    28141 ttcttgtttt aatgttttga tcgatgtagc aactccgctg acttgcggaa agtaggtatc
    28201 ggtaaaaaag ccgattttca tgtttttcgc ctccaaacta gtccttgacc tgatttactt
    28261 tttttaattt gctagagact ttttcattca gtacactttc gtatacattt ttaagttctt
    28321 caccgatttt attgatactt cgctcttttg ctgtttgata accggcttgt gtcgtactcg
    28381 gtagttcttg attggtaatc tgttggatca gtcggataaa ttcttcgttt gtcttcccca
    28441 tatagcagtt cttgctatct tctaaccagc cattatacac tggaatatct cgaacaagta
    28501 cgttttgttt gcttgctaaa gcttctaaaa caacgatccc ttctgtctct tcataagaag
    28561 ggaaaaagaa tagatctgca cctgaatacg caccttctat gatctctcca cgaatgtaac
    28621 caggaaacag gacattttca ggatgatctt cttttacaat tttccggatg ttccgaggaa
    28681 tcgagtacat gggaacatga ccaaaccaaa taaatgtgta ttcgggcatc ttcttagcaa
    28741 tttctacgaa atcaatgatt cctttacgtt cgaaaaacaa accgacacaa atgattactt
    28801 tttgatccgg agacagtttg aaatattctc tgaacttttg ttctttcatt ggatcagggc
    28861 gatatttctc taagtcgatc ccattggaaa tagcttgaat cggcacacgg atactatagc
    28921 tttccaacaa ctgttttgaa taaggtgttg gcgtgatcag atagtccgct ttttggtaca
    28981 atcctaccag atattttttt acgataggag aaagctggtt cgatccgata aaggaatttc
    29041 ggaaatcttc ctctgtagaa tgcgcatgat agacaacttt ttttccttct cttctggctt
    29101 tattgaccat attatgacta ttgattccgt atgtattgat gtgcaggatg tcataatctt
    29161 ctttttcatc tagtgtataa gagataccga cttccgtcag agcacgtttt tgatgatcaa
    29221 gcgcacgccc gattccagat tttgcaagca tcttttcact ttcaaaatat aataatacct
    29281 tcaccttacg tcccccttca cttatatagt ataacatagc ctacaaactg tcactcaaac
    29341 ttaagcccta tttttatcta ttcttactga caaaaacgtt ttcttaaaaa agaacaaaaa
    29401 aactggcgta gctcttttac aagccacacc agcttttgat acgttgttta ttttacatat
    29461 tcatgaacaa gttcaacgac ttcttccatc gtatcgcatt ctttcaatgc acgatcagca
    29521 agttcagcca ttttcgctgt gtccaagcgt ttcattaaac tacgcgtttt aagaatagat
    29581 gtcgcactca ttgagaactc atctagaccc atacctacta gcaatggaac agctgtttga
    29641 tctccagcca tttctccaca catacctgcc cattttcctt cagcgtgtgc tgcatcaatt
    29701 acatttttga tcaaacgtaa aattgatggg ttatatggtt ggtacaagta agaaacgcgt
    29761 tcgttcatac ggtctgctgc cattgtgtat tgaatcaaat cgttcgtacc tacagagaag
    29821 aaatctactt cttttgcaaa cttgtctgct aagactgctg ctgctggaat ctcgatcatg
    29881 atgccgactt ggatcgtatc agatacttcc ttgccttcac tgatcaattt ttgtttttct
    29941 tcttcaaaga tcgcttttgc tgcgcggaat tctttgagtg tagctaccat agggaacatg
    30001 atacgtaagt taccatgaac agatgcacga agcaatgcac gcatttgtgt acggaacatt
    30061 tcgtcgcctt gttcagataa actgatacgc aatgcacgat atcctaagaa tggattcatt
    30121 tcatgaggta attgcagata tggtagttct ttatctccac cgatgtccat tgtacgaaca
    30181 actacaggtt ttccttccat accttctaat acagctttgt aagcttcata ttggtcatct
    30241 tctgttggga agtctggaga atccatatac aggaattcag tacggtacag accaactgct
    30301 tctccaccat tattatggac accaactaga tcttttggtg ttccgatgtt tgcagctaat
    30361 tcaaaatgct tgccgtcagc tgtgacagtt tctgcatttt ttaatttttc ccattctgct
    30421 ttttgtgctg cgtaatctgc acctgctttt tcaaattcag ctttttgttc atctgtagga
    30481 tcgatgatta catcgccttc gatcccgttg acagccaaaa tatcgccttc tttgacttta
    30541 gcagtaattt cttttgtacc aacgattgct ggaatttcta aagaacgtgc catgatagca
    30601 gaatgtgaag tacgtccacc aatgtctgtt acaaacgctt ttacaaaatt gcgatctaat
    30661 tgtgctgtat cacttggagt caagtcatgt gcaacaacga ccacttcttc gttgatcatt
    30721 gatggatttg gtaaagtaac gcctaataag tgcgctaaaa tacgtttagc aacatcacga
    30781 atatctgcag cacgctcttg catataagca ttatcttcca ttgcttcgaa cataccgata
    30841 tacatatccg ttacttcttt tagcgctgat tcagcattta cgctgttgtc tttaatattt
    30901 tgcttgattt gacctaccat ttctggatct gaaagaacca tcaaatgggc atcaaatact
    30961 tgcgcttctg cttcacctaa gctttgcgct gctttttcac gaatttgctg caattcttca
    31021 gtagatttag ctaatgcagc atccaaacga gcttcttcag ccgcagtatc ttctactgtt
    31081 gccttactga atgttaaatc cggttgaact agcaggtaag cttttgcaac ggctacaccg
    31141 tcactagcgg caatcccttt tagcatttca accattattc agataagcct tctttcttca
    31201 ttgtttcaac gattgctgcc attgcatcag cttcgtcagc accttctgca gtaatagtaa
    31261 catcagaacc ttgacctaca cctaaagaca taacgcccat gattgatttc aagtttactg
    31321 atttaccttt atactctaag tttacatcag agttaaattt gcttgctgtt tgtactaata
    31381 gtgtagctgg acgagcgtgg atcccagttt ctgctactac gtgaaattct ttcttttcca
    31441 tattaatcgg tctcctttaa gtgagtgttg tttttatgtg agggttttct tttttagtaa
    31501 ttcagagaat cctaacaaat gccacccttt catctgtaaa gattaacatt ttttttcgtt
    31561 agatacaaca gttttcattc aatttcagat aaaagtattc aaattattcc ggattctttt
    31621 tcatcctttt cttcacattt tataacaaac gtgtttattt tctttttctt gtcgctttct
    31681 cttctcctga cgtttatcat tctttccttt gaccctcttt gttttcatat gaaacgaatt
    31741 acttgcttcc ctttttattt ttagtataat aagagtcaaa ggtcaggaat ggtcaataga
    31801 gatttcacct tttctacctc ataaatctct taaaaaacta ggggttattc ttttacctca
    31861 tgaaaaacga gatgtaaaat atggatgtac ccatttttgt gaaaggacgt gaatgattca
    31921 tgctttgtca aaactgcggc aagaatgagg caacgattca cttgtatgcc actgtcaatg
    31981 gtcagcggac acaactcgat tactgtcaga gctgttatca aaaaatcaaa aatcaacaaa
    32041 atggaggtct gactaatatg gcccaaaatg atccatttgg atttggcagt ttggacgatc
    32101 ttttccgttc catgtctcgc caaatgcaac agcaaggaaa ttatgagcaa acaccaccaa
    32161 cacaatttgg cggaaacaac aacttcaatg gtggtcagcc acctcaaggc aatacagaag
    32221 gtttattggg agaatacgga atcaatatca cagaacaagc acgacaaggt gatatcgatc
    32281 ctgtgatcgg acgcgatgac gaaatcaaac gagtcattga aattttaaat cgccgaacca
    32341 agaacaatcc tgtcttgatt ggagaaccag gtgttggtaa aacagcagtt gttgaaggtt
    32401 tagcacaaaa aatcgtagat ggtgatgtac cgcaaaaatt aatggacaaa gaagtcattc
    32461 gtttagatgt cgtatcttta gtccaaggta ctggtatacg aggacaattt gaagaacgta
    32521 tgcaaaaatt gattgaagag atccgtcaag cagaaaatgt gatcctattc atagatgaag
    32581 ttcacgaaat cgtcggtgct ggttctgctg gtgatggaaa tatggatgct ggtaatattt
    32641 taaaaccagc tttagctcga ggagaattgc aaatggttgg tgctactacc ttaaatgagt
    32701 accgcatcat cgaaaaagac gctgctttag aacgtcgtat gcaacctgta cgggtagatg
    32761 aaccaactgt cgaagaaacg atcagtattt tgaaaggttt gcaaaaacgt tatgaagact
    32821 atcatcatgt aaaatatact gatgaggcca tcgaagcagc cgcaacttta tccaatcgtt
    32881 acgtccaaga tcgcttcttg ccggataaag ccatcgattt gctagatgaa acaggttcta
    32941 agaaaaactt gacgatccaa atcgttgatc ctaaaacaat cgagaaaaaa ctgcaagaag
    33001 cagaagaaca aaaagtttta gcttctcgag aagaagattt cgaaaaagca gcttattatc
    33061 gcgatcagat caataaactt caaaaaatga aagaacgtca gctcactgaa gaggaaacac
    33121 ctgtgattac cgaaaaagat atggaaaaaa tcgttgaaca acggacaggt atttcagtgg
    33181 gtgaattaaa agaaaaagaa cagacacaac tgaaaaactt agcagatgac ttgaaagctc
    33241 atgtcatcgg acaagataac gctgtagatc gagtagcgaa agcgattcgc cgaaaccgtg
    33301 ttggattaaa caaacagaat cgtccaatcg gctccttctt atttgttgga ccaactggtg
    33361 taggtaaaac agagcttgcg aaacaacttg cctacgaact attcggttca caagattcaa
    33421 tgattcgttt cgatatgtct gaatatatgg aaaaacatag tgtctctaaa ttgattggtt
    33481 ctcctccagg ttatgtcgga tacgaagaag ctggtcaatt aacagaaaaa gtacggagaa
    33541 atccttacag tcttgtcttg ttagatgaag tagaaaaagc tcatccagat gttttgcata
    33601 tgttcttgca aatcttagat gatggtcgat tgacagatgc acaaggaaga acggtcagct
    33661 ttaaagatac gatcatcatt atgacaagta atgccggaac tggtaaagta gaagcaaacg
    33721 ttggttttgg tgcggcacgc gaaggtgtta ctcgttctgt attgaaccaa ttaaacaact
    33781 actttacacc agaatttctg aaccgttttg acgggatcat cgaattcagt gcattatcaa
    33841 aagaaaactt gatgacaatc gtcacactaa tgctagacga tgtcaaccaa atgctggcag
    33901 cacagcaact tcacatcgaa gttcctacga atgtcaaaga aaaattagtt gacttaggct
    33961 atgatccatc tatgggtgct cgtcctcttc gacgcacgat ccaagaacaa atcgaagatg
    34021 gtatcgctga attttatctt gatcaccctt ctatccatga gttgaaagca aaactggata
    34081 aagatggaaa aatcatcgtt acttctaaac cagaacgttt ggctaaagaa tcagccgaag
    34141 aaacggctga ataaatccag ttttatagcg tgagacggta tttctttgag aaatgccgtc
    34201 tcattctttt ttcagaaaag tcaaaatact gttcatttta taaaaaagaa attataataa
    34261 gagaaacaag caattaaaaa tacataaaag aggtgagcgc catgaaattg gtaaatgtaa
    34321 caaacagcca ttcccgtctt gtattaaacc agcttgaaaa cactgatgcc catctagtca
    34381 aggtctacac agccggaaat accactattg tctacactga agcccctgaa cataatgaaa
    34441 ttctgctgat caatgacaaa cgtaaaatcc agccgaaaga aatcgaagat gctaaaaatt
    34501 atttcttaga taaaatcaaa gacgccgttt accacgagga tgaaatcaaa gtaatcgaat
    34561 tggcaggcct tgttgaaatc tcgattccaa aacatgaatt aggcgctaca ttagccgtgt
    34621 aaacaaattt tgaacataat aattaaagag cgtgggacaa aagtaaagaa cacttttgtc
    34681 tcacgctcta aacacgtata aacggcggaa gcagaagcaa ctccttcgga aat
//
LOCUS       DAZUMM010000023        34282 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000023 DAZUMM010000000
VERSION     DAZUMM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.023"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            151..1917
                     /locus_tag="ACG8E6_001044"
     CDS             151..1917
                     /locus_tag="ACG8E6_001044"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFS5061482.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2019..3278
                     /locus_tag="ACG8E6_001045"
     CDS             2019..3278
                     /locus_tag="ACG8E6_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5061483.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3425..4726
                     /locus_tag="ACG8E6_001046"
     CDS             3425..4726
                     /locus_tag="ACG8E6_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5061484.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4729..5580
                     /locus_tag="ACG8E6_001047"
     CDS             4729..5580
                     /locus_tag="ACG8E6_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS5061485.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5593..6408
                     /locus_tag="ACG8E6_001048"
     CDS             5593..6408
                     /locus_tag="ACG8E6_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFS5061486.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6559..7725
                     /locus_tag="ACG8E6_001049"
     CDS             6559..7725
                     /locus_tag="ACG8E6_001049"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5061487.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            7955..8602
                     /locus_tag="ACG8E6_001050"
     CDS             7955..8602
                     /locus_tag="ACG8E6_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS5061488.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8686..9630
                     /locus_tag="ACG8E6_001051"
     CDS             8686..9630
                     /locus_tag="ACG8E6_001051"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFS5061489.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9765..11216
                     /locus_tag="ACG8E6_001052"
     CDS             9765..11216
                     /locus_tag="ACG8E6_001052"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFS5061490.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11237..12073
                     /locus_tag="ACG8E6_001053"
     CDS             11237..12073
                     /locus_tag="ACG8E6_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS5061491.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12073..12732
                     /locus_tag="ACG8E6_001054"
     CDS             12073..12732
                     /locus_tag="ACG8E6_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5061492.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            12858..13547
                     /locus_tag="ACG8E6_001055"
     CDS             12858..13547
                     /locus_tag="ACG8E6_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061493.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13553..14509
                     /locus_tag="ACG8E6_001056"
     CDS             13553..14509
                     /locus_tag="ACG8E6_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFS5061494.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14553..15065
                     /locus_tag="ACG8E6_001057"
     CDS             14553..15065
                     /locus_tag="ACG8E6_001057"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5061495.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15321..15869)
                     /locus_tag="ACG8E6_001058"
     CDS             complement(15321..15869)
                     /locus_tag="ACG8E6_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS5061496.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(15958..17139)
                     /locus_tag="ACG8E6_001059"
     CDS             complement(15958..17139)
                     /locus_tag="ACG8E6_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFS5061497.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17375..18883)
                     /locus_tag="ACG8E6_001060"
     CDS             complement(17375..18883)
                     /locus_tag="ACG8E6_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFS5061498.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19042..19233
                     /locus_tag="ACG8E6_001061"
     CDS             19042..19233
                     /locus_tag="ACG8E6_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061499.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19436..20353
                     /locus_tag="ACG8E6_001062"
     CDS             19436..20353
                     /locus_tag="ACG8E6_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFS5061500.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20346..21419
                     /locus_tag="ACG8E6_001063"
     CDS             20346..21419
                     /locus_tag="ACG8E6_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061501.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21567..22085
                     /locus_tag="ACG8E6_001064"
     CDS             21567..22085
                     /locus_tag="ACG8E6_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061502.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22066..22458
                     /locus_tag="ACG8E6_001065"
     CDS             22066..22458
                     /locus_tag="ACG8E6_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFS5061503.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22534..23469
                     /locus_tag="ACG8E6_001066"
     CDS             22534..23469
                     /locus_tag="ACG8E6_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5061504.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23602..24381
                     /locus_tag="ACG8E6_001067"
     CDS             23602..24381
                     /locus_tag="ACG8E6_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS5061505.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24374..24691
                     /locus_tag="ACG8E6_001068"
     CDS             24374..24691
                     /locus_tag="ACG8E6_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS5061506.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            24935..26545
                     /locus_tag="ACG8E6_001069"
     CDS             24935..26545
                     /locus_tag="ACG8E6_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5061507.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26702..27550
                     /locus_tag="ACG8E6_001070"
     CDS             26702..27550
                     /locus_tag="ACG8E6_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5061508.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            27781..28260
                     /locus_tag="ACG8E6_001071"
     CDS             27781..28260
                     /locus_tag="ACG8E6_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFS5061509.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            28293..30521
                     /locus_tag="ACG8E6_001072"
     CDS             28293..30521
                     /locus_tag="ACG8E6_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5061510.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            30650..32293
                     /locus_tag="ACG8E6_001073"
     CDS             30650..32293
                     /locus_tag="ACG8E6_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS5061511.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(32366..32911)
                     /locus_tag="ACG8E6_001074"
     CDS             complement(32366..32911)
                     /locus_tag="ACG8E6_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFS5061512.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(32934..33419)
                     /locus_tag="ACG8E6_001075"
     CDS             complement(32934..33419)
                     /locus_tag="ACG8E6_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5061513.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(33409..34221)
                     /locus_tag="ACG8E6_001076"
     CDS             complement(33409..34221)
                     /locus_tag="ACG8E6_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFS5061514.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
BASE COUNT        11231 a         6145 c         6879 g        10027 t
ORIGIN      
        1 taaaagtttt aagtctatct atttggcaag tttaaaaaat aaaaattcgg tatcaaaaaa
       61 ctgttaccga taacattttt gaaagcgttg tcttttgctt ctctatcact tatagtgatg
      121 acgtcatatt aaaacacaag gaggaaatca atggatacag ctgcaattta tcatcgaccg
      181 gaaagtgaat atgcatttct atatacgaat acaagatttc gtattcggct tcgtactagg
      241 aaaaatgata tcaaaaaagt atatgtgcta tgtggagatc catataccat aacaactgaa
      301 aaatggtatc aaaaacaacg accaatgaaa aaatgtctaa gtaccaatgt tcatgattat
      361 tgggaaattg aagtgacgag cgaaacaagt cgcttacagt atgcgttcca tgttgttggg
      421 gaagataaca cggactgttt ttacggtgat caaggaattt ttccttatcg agaaaatgtg
      481 attactgaac caaattttta ttttcgaata ccttattttc atcaaatcga tcgattcact
      541 attccagaat gggtaaaaga aacagtatgg tatcaaattt ttcccgaaag atttgcaaac
      601 ggggacaaag caaatgaccc cgaacacact ttaccttggg gaagtaaaga tccagaccga
      661 gaagactttt tcgggggaga tttgcaaggt gtgatggatc atttggatta cttgacggac
      721 cttggcatca atggcatcta tttttgtccc atatttaaag ccacatccaa tcataaatac
      781 gatacgattg attattatga ggtggatccc gcttttggcg ataaaaaact tttaaaaaac
      841 ttgattgatg aagcacataa aagagggata cgtatcatgt tcgatgctgt attcaatcat
      901 atgggtgtcc attcaccaca gtggcaagac gttctcaaaa acggagaaaa atcgatctat
      961 aaagactggt tccacatccg ttttttccca gtagattctt atcagatgac tgacttacca
     1021 gaaactgctg aaaatatccc atatgatact tttgctttta ctccattcat gccgaaacta
     1081 aatacagcaa atccagaagt gcaaaaatat ttgctggata tcgcaactta ttggataaaa
     1141 gaattcgata ttgatggatg gcgtcttgat gttgctaatg aagtcgatca tcatttttgg
     1201 aaaaaattca gagaagcagt tacagaaatc aaaccagaca tctatatcct tggagagatt
     1261 tggcattcct ctcaagcttg gctgcaaggg gatgaattcc atgcagtgat gaattatgca
     1321 tttacagatt cgattaaaga ttattttgca aaaaagaaaa tcacagcttc acaaatggtc
     1381 agtggaatga atcatcagca aatgctttac agagatcagg tgaacgaagg aacattcaac
     1441 ttattagatt ctcatgatac tgccaggatc ttgacccttt gccaaggtaa caaagaattg
     1501 atgaagtctg tgatggcctt tatgtttctt caaaaagggt ctccttgtat ctattacggg
     1561 acagaaatag ggatgactgg tgaagatgac ccggattgcc gcaaatgtat gatatgggag
     1621 aaggaggaac aagatctaga gctattcggt tttataaaag aacttgtttc tctaaggaaa
     1681 cagttgtcta agattatttc tgaaggttct actcagtggc taatagtaaa tgatcgtgaa
     1741 gataagctct atttcacacg gaaactagag ggacagatca tctatgttta ctttaatcag
     1801 tcaaaagaac cttgggtagt agaacaggaa aatgaggtga ttcttagtca aaattgtcag
     1861 ttgttagaag acgggaaagc agagatacga caatacggat ttttgatcat gtgttgaaaa
     1921 ctgttaccgg taacaacaag aaagcgtttg caaatgaaga tttgtgccca ttataataag
     1981 ggtgtaacag cacgttgaat acaaaggagg aaatgaagat gaaaaaaaga agtgtgaaac
     2041 agttgggttt agggttaatc tctttaggaa tcttaggcgg attagcggct tgtggcaatg
     2101 gtaactcttc agcagaaggg gaagccgatg aaggaaaaac gttaacagtt tccgttgatt
     2161 ctggatataa agattatata aacgaaataa aaaacacttt cgaaaaagaa aacgatgtga
     2221 aaatcaagct agtagaaaag gacatgttcg atcagttgga atcattagcg ttagatgggc
     2281 ctgctggaaa gggacctgac gttatgatgg cagcatatga ccggatcggt gcgttaggac
     2341 agcaaggaca tctggcggaa gtaaaactgg gaaatgaagc agcttacgat gaaacagaca
     2401 aagcccaagt gacttatgat ggaaaaatct acggagaacc tgcagtaatc gagacccttg
     2461 ttttgtacta taacaaagat ttagtcgata cagcaccatc aacgtttaaa gatttggaga
     2521 atctatccaa agacagtcgt tttgcttttg agtcagaagc aggaaaaaat acaggatttt
     2581 tagctaaatg gactgacttt tattattcct atggtttaat tgcaggttac ggtggctatg
     2641 tatttggtga tgatggcaca aatccttcag atatcggttt gaataatgca ggtgctgtag
     2701 aaggaatctc ttatgcaaca gattggttca aaaatgtttg gccaaaggga atgcaagaca
     2761 taaaaagtgc aggagacttc gccagccagc aattcatgtc caataaaact gcagcaatca
     2821 ttgacggtcc atggcaagcg caaacataca aagaaaacaa tatcaattat ggtgtagcca
     2881 aaattcctac tctaaataat ggacagcctt atcaaccgtt tggcggagga aaaggatggg
     2941 tagtcagcaa ttatgctaaa aacaaagacc tctctcaaaa atggcttgat tatgtaacga
     3001 atcaggaaaa tcaagaaaaa ttctttgaaa tggtaaatga aatccctgca aaccaacaag
     3061 ctagggaaac agcaaaggct aaaaacgatg aattaacaac agctgtgatt gaacaatatg
     3121 aaacagctca agcgatgcca aacatcccag aaatgggcga agtttggaca ggtgcagaaa
     3181 acttgatgtt cgatgcagca tcaggttcaa aaacaccaaa agaatccgca gatgaagcag
     3241 taaaaacaat cagtgaagcc atagaacaaa aatactgatt aaaatgataa ccgtccattt
     3301 ttataaaaaa tggaacaggt gcttcagata aaagtggcaa gagcgagatg gaaaattatc
     3361 ttgctcttgt tgccattatc ttaaaagcag tttgcggttt agcaagttta gagggggcat
     3421 tcagatgcag caagataaaa caagcaatat caacgaaaaa aaaatcacgc tttgttcact
     3481 tattccaggg atggggcagt ttgtaaatgg ccaagctttt aaaggtatcg tttttttcgc
     3541 catattcgtc ctattcattg gtcagatgct tctgggcgga tttcatgcat tggtaggatt
     3601 ggttactttg ggaagtatac caatggaaga tcattcatta tttattttga ttgaaggcac
     3661 tttgcaattc atcattacaa tcattttctt gattttttac ggtttgaatc ttttagacgc
     3721 ttatcgagta gcgaagatgc gtcaagaggg caaaaaaatc aatcggacga tgaaggaagt
     3781 catacataat ataggtgatg aaggttttcc ttatctattg acacttcccg cttatcttct
     3841 catgattttt acgatcattt tcccagtcct agttacattg ttcactgcat ttactaatta
     3901 tgacttcaat catattccac cagccagttt aattgattgg gtaggggcta aaaacttttt
     3961 tagtatcttc tttttaagct catatcgtaa tacttttttt gcagtctttt cgtggaccgt
     4021 gatttggact ttatgtgcaa caactttgca aatcaccctt ggtattctca cagcggttgt
     4081 tgctcaccag aaatttatta aattcaaacg tattttcggt atcattttct tattaccatg
     4141 ggcggtccca gcttttatta ctatcatgag tttttcgaat atattcaacg atagcgtagg
     4201 tgccatcaat actcaagtga ttcctttcat aaaccagctt ccttttgtag atatcagtgc
     4261 aatctcttgg aaaacggacc cgaactggac gaaagtagca attatcttag tgcagggctg
     4321 gttaggattt ccctatatct atgtgatggt tacaggaatc ttacaatcga tttcagaaga
     4381 cttatatgaa gcagcaaaaa tcgacggagc aaatgcatgg cagcgctttt caaacatcac
     4441 gcttcctgct gtcttcctag tggcagcgcc aacatttatt acacagtata cggggaattt
     4501 caataatttc tccatgatct atctgtttaa caatggcggt cccggaagcg taggcggagg
     4561 agctggttct actgatattc ttatttcctg gatctataaa cttacgactg gaacttctcc
     4621 gcagtattct atggcttcag caatcacgtt gattatctcc gtcatcgtta tctgtgtgtc
     4681 attgctggta tttaaaaaga caaatgcgtt caatatgggg gattaacaat gaaaaaattt
     4741 cattcgagtg ctcgattcca aaaacgactt tctcactttt ttacttactt ttttctttta
     4801 atactgtcag tcattatcat ctatccgctt ttgatcacag caagtactgc cttcaagtca
     4861 ggaaatattg cagcttttag tttagatctt tcaagtgact ggactttagg aaactttact
     4921 cggctattca gtgaaacact ttacgggact tggtataaaa atacattgat catttctgtc
     4981 ctaacaatga tcgtacaagt gactgtagta actttggcgg gctatactta tagtcgctat
     5041 cgttttatcg gccgcaaatc aagtttgatg ttctttttag tgattcagat ggtaccgact
     5101 atggcagcat tgactgcctt ttatgtcatg gctttcttat tagaagcgct cgataagtac
     5161 tggttcttga caatgatcta tataggcggt ggaattccaa tgaatacttg gctgatgaaa
     5221 ggatactttg acactgtacc aatagattta gatgaatctg ctaaacttga tggggcaggg
     5281 catttcagga tttttttcca gattgtatta ccgcttgtca aaccgatgct cgccgttcaa
     5341 gcattatggg cttttatggg accttttggg gatttcatgt tagctaagtt cctgcttcga
     5401 acacctgaaa atcagactgt tgctgtcggc ttacaaacat ttatttctga tgtgacaaac
     5461 caaagagtct ctctttttgc tgctggggca attttgattg cactaccgat ttgtctatta
     5521 ttcttctttt tacaaaagaa ctttgttacc ggcctgacat ctggcggaac aaaaggatag
     5581 gagcagagta tcatgcaaac atttccgtta aactattttt ccgcattacg tacgccaact
     5641 caagtgtttg cgggacgcaa actattatca tggcctaaat ttttcttgat atttgtattt
     5701 ttagtttcct tgatggtcat gcctgtgact cttttttatg ccaatcagat ccaagcgatt
     5761 ccattagaac aatttctatc cgttcattcg ctgatcgatg aacaaggaac ccaaaaattt
     5821 tctgaacttg aattatctga aactgggtta caagcatcgc aacaaacgat agctgtgacc
     5881 cctgaaattt tggtaggtgt gtctttatct gagaaacaac aatccgatca tggaactttt
     5941 attgattttg agaaggaaca atgggtgatc caacaaaagg ataaatctgg tatacgccgc
     6001 tatacgatga attatagccc atctttccaa ccagattctg tacgtacgcc cgaagatttc
     6061 caacgatttt tagaaagaga attttatgcc agtaatcgtc caacaatcat tttatcttat
     6121 agtttaagtc ttggtcttgt cctatttgtc atgacgagtc tcattttatt cggggcttcc
     6181 tttttcttgt ggatgacaag aaaaagccaa ctttcatcca ttcatacatt caaagagagt
     6241 gcaaatttga tgctgaatgt tatgggaata ggttccatgg cagcagcagt tgtagggttt
     6301 atccactttg actttatctt gatgctaagt gtccaaacaa cgataactgt cttgctgctt
     6361 ttatgggttt ttgtcaaaac aagattcaaa gataagcgtg tcgaatgaca agtttaagcg
     6421 atttatgcga caatgaatgt gaaaaaaatg aaaaataaag tagtttttga aaaatttctg
     6481 aaaaaattaa acttcttagt tgattctttt cttttttagt attaacgtaa tagggtagtt
     6541 tttttaagga ggaaaatcat gttagagaaa actttataca atcaattatt ttcacgttct
     6601 ttctcgctcc ctgtcgaagt aaattattgg gacggtacaa cgaaacaata cggagatacg
     6661 gacactcctc cccaaatcaa aatcaatatc catgaagaaa tcccaatgaa agagattaca
     6721 agtaacgctt ctttggcact aggagaagcc tatatggatc atcgaatcga gatcgaggga
     6781 agtatccaag cattgatcaa cgatgcttat cagcaagcaa acagcttttt acgaggaaaa
     6841 gattatctaa aatggctgcc aaagaaagaa aaacatacga aacaaaaaaa caaagaagat
     6901 atccatgcgc attatgactt aggtaacgac ttttataaac tttggctaga tccaactttg
     6961 acttattctt gtgcttattt caatacacct gaagatacgt tggaacaagc acaaatcaat
     7021 aaagtccatc atatcttaga caaactcttt atcaaagaag gcgaaacgtt gcttgatatc
     7081 ggttgtggct ggggaacatt gatgttcact gctaccaaag agtataatgt caaggcaact
     7141 gggatcacac taagtgaaga acaatacgat ttgattacca aaagaataaa agaagaacat
     7201 ttagaagata aatgccgtgt attattaatg gattaccgcg aactaaaagg cgaaacgttt
     7261 gatcatatca caagtgtagg tatgttcgaa catgtaggtt ctgaaaatct tgaaggttat
     7321 ttcaaagtgg taaaagattt attgaaacca aaaggaacag ctttgatcca tggaatcagc
     7381 cgacaacaag gcggtgcgac aaatgcttgg atcaataaat atattttccc tggcggttat
     7441 gtccctggtg ttactgaatt agttgataat attacaaaaa acgatttgca actgattgat
     7501 ttggaaagct tacgtcgtga ctaccaattg acattagaac attggacaaa gaattttcat
     7561 caagtccaag atcaagtcac gaaagaaatg ggtgattcct tctatcgaat gtgggatcta
     7621 tatttacaag cttgtgcagc ttcatttgaa tcaagtaata tcgatgtgat tcaatatttg
     7681 cttgttcaac ctggaaacaa tgacatccca atgcatcgtg cataattgag gcaaaagtac
     7741 tgtttaaaga ctatttaaaa agaagctaag ttttatgatg ctccagaagt gaactttttt
     7801 gtctaacttc taggattgtc tctaaagctt agtttctttt tctttatatt ttcataataa
     7861 aagtttggtt ctttaatttt taaacatgac agaaaatttt ttcttcgtta tacttgtaat
     7921 agaatactat tttataacaa aggggatcga atcaatgaaa aaaactgtta tttatactag
     7981 tttatttgca gctttgtttt tagctgcttg tcaatcaaat accaatcaag gcacatctgg
     8041 gaatacgtct tccagtagcg aacagcgctc ttcacaggta tcggactcct caacaaaagc
     8101 aagcacatct tcgtctagcc agacaaaaag ttctagtgaa gcaagtactc agtcagatat
     8161 caaagtgtct gcagctcgtg cgatcgaatt gttccaagat aaatacccag atactgcaat
     8221 tactagtttg gaattagata gtgattgggg atcttacttc tacaagatag aaggtgtcga
     8281 tgatcaaaaa gaatacgaag caagaattga tgctcaaaat gaaaaaatgg agacagagaa
     8341 tccagaaact ctagatcgag atgaacaaaa tggtcaaaag aaaaaagaag atggtctgga
     8401 tgttgataaa ttgatcacga ttgaggaagc agcaaaaatc gccgtcgaca aagtaggaag
     8461 cggtacggca acagattggg atttggataa agatttaggt actacttatt gggaagtcaa
     8521 agtaaaagac ggcaatcaaa gtacatcagt caagatcaat agccaaacag gcgaagtact
     8581 gtcaactgaa caagatgatt aagaatttct tcggaaaaca agagttgatt tttcttttag
     8641 tcatgataca ctagacaaaa taacttgttt gaaggagcga tttacttgag tttagaaacg
     8701 acctttgcca atcatatctt tgccaatcct ttgatgaatg catctggtgt ccactgcatg
     8761 actacccagg aattagatga actggctcat tcagaagcag gagcatttat cactaagagc
     8821 tgtacgatca atgaacgaaa aggaaatccc gaaccacgct attttgatgt tcctttaggg
     8881 agtatcaatt ctatgggact tccaaactta ggtttttctt attatctaga gtatgctctt
     8941 gcgtatgaaa aagtgcagga aaaccaaaat caaccgttat ttttttccat tgccgggatg
     9001 agtgtccagg aaaacttgga aatgcttgag aaaatagaaa aaagtgggtt taatgggatc
     9061 actgaattaa atttatcttg tcccaatgtg cctggaaaac cacaactagc ttacgatttt
     9121 gaggccactt atgaaacact gaaagaagta ttcagcatct tttctaagcc gctaggaatc
     9181 aaattgcctc cttattttga ttttgctcat tttgatcaga tggctgatat cttgaatcag
     9241 tttccattga cctatgtcaa tgctatcaac agtgtgggca atggtttgta tatcgataca
     9301 gaacaagaag cagtagtcat caaaccaaaa gaagggttcg gcggcattgg cggagagtat
     9361 atcaagccga ctgctttggc gaatgttcgc gctttttata cacgtttgaa accagaaatc
     9421 caaatcattg gtacaggtgg gattcggaca ggacaagatg catttgaaca tctgctatgt
     9481 ggcgcaagca tgttacagat tggtacagag cttcataaag aagggccgga aattttttct
     9541 agaatcataa aagaattaac gcagattatg tcagaaaaag gatatacgtc aattgacgaa
     9601 tttaaaggca aattacgcac catttcatag caaagagacg aaggtcatga ttgatcttcg
     9661 tcttttttta taaaaaacac tcgtctgttc attcctgtgc aagcgtactc attgtatgtt
     9721 agacgaactt gtgggaaaat aacagaggca aaaaggagga taatatgaat gatgcaacag
     9781 aattagcaaa agagcttaaa gaaaaagaat ttagttttca agaactagtt acggatatac
     9841 agaaaaaaat aaagaagcaa aatcatgaat tgaatgcgtt tgtcgcgctt gaaccattcg
     9901 agagtaattc ccacgagcta ttgattcccc agtcagaaaa agggccattg gctggtatcc
     9961 catttccttt aaaaatgctc ggtcaagaaa aaaagggatg gctagctact tcaggatctc
    10021 gtttatttga aacacaccga gcttctcata catctaatta tgtgcggcaa gcagaagcga
    10081 taggtctagt tccttttgga caaaccaatg cgccagagtt tggtttcaaa aatattacag
    10141 acccagtcat ctatggccca gcaagaaacc cttggaacct tgaatgtaca ccgggcggat
    10201 caagtggagg agctgctgct gcagtttcat caggaatcgt tcctttagct ggtgctagtg
    10261 atggcggtgg ttctattaga atacccgctt cgttttgcgg attgattggg ctgaaacctt
    10321 cgcgagggac gatgccagtt ggtccatatg cttggagagg atggcaagga gctgctattg
    10381 atttcggctt gacagtttct atgagagaca cggaggcttt attttatggg atgagaagta
    10441 tccattccgg tgcgccatat caagtatcgc cagttgagtg gcaaacacat ccaagaaaaa
    10501 aacggttgaa gatagctttt tgtgttgatt caccgatcca ctcacagatt agtgaagagg
    10561 cagtacttgc tgtaaaaaaa gctgtagatt tccttggaaa agaagggcat gatttgacag
    10621 agattcctta cccactagat ggtcgtaaac tgatcacgtc ttattatcag atgaatggcg
    10681 cagaaacagc agcaatgatc cgctcgattg aaacaggcat ccagcgagct gtcagaaaag
    10741 aagaacttga acccatttct tggactttgt tccaatatgg tcagaagctc tctgccgcta
    10801 cttacatcga ttctctgcat gtatgggatg aagcagcaat tgtcatggaa aacctacatc
    10861 aagaatacga tctattcctt tcaccaacga ctgcatttac cgcccctaaa atcagcgaag
    10921 atttgcaaag tgatgcgata agagcacaga tggaacacat cagtgattgt tctgaagtag
    10981 aaggtttaga cgttgtttct tccatgtttg aggagagctt gaagatcaca ccttatacac
    11041 agttggctaa tctgactgga caaccagcaa tcagcttgcc tactcatttg acaaaagaaa
    11101 acatgcctct aggaatccaa tttatggctg ccagaggaag agaagatttg ctatttcaaa
    11161 tcggctatct atttgaagca aatcatcagt tccatttacc aaatccatgg aaatagcaga
    11221 aacaaggaga agaagaatga agaaaaaaag attgtttgct ctaataacta gtgccttttt
    11281 tattttaagc gcttgcggta cagctgccgc taatcaggaa gataacagct ggagtaatgt
    11341 agaaaaaaag aaagaattag tagtagcgac ttctggcaca ctgtttcccg catcttatta
    11401 taatgatcaa aatgaattag ttggttatga tgtagatttg gcaaaagagg tggctaaacg
    11461 gttaaatgtc aaaatctcct ttaaagaata caacgtggat ggacaaatca cttctgtttc
    11521 aaaaagagaa tcagattttg ctgcgaacga ttttggtatc acaaaagaaa gagaaaaaaa
    11581 attctccctt tcagagactt tcaaatattc atttgacagt atgatcgtac gaaaaaaaga
    11641 tgattcagga atccactcac ttgaagatct taaagggaaa aaagcagctg gcgaacccaa
    11701 tactagctat atgcgtttag cggaaaaaca cggagctaaa ttagtcacat atgacaatgc
    11761 cacgaatgat cagtatttaa gcgatgtagc aaatggtcga acagatgtta ttctaaatga
    11821 ttattatctg cagaaaatgg ccgtatctgc tttaccagat atccctgtaa aaatactgga
    11881 gaacgtttat ttcaatgaaa acgaaagtgg ttttttattg aaagaaggaa atgatgctct
    11941 gaaaaagaaa atcgatggcg tattaaaaga gatgaaagaa gatgggacga caaagaaaat
    12001 atctgaaaaa tattttcaaa ctgatgtttc agtcaaaccg aaagaaacga tggctgagtc
    12061 ggtgagtgag tgatgtactt tcctctaatt gatttttccc tcatgtggac ttcacttcct
    12121 ttcgtactgc aaggcttggt atacacatta gggattggct tcgtgtcgtt tcttttagga
    12181 aatttggtag ggttgctttt gacagtttta ggtcttttgg attggctgcc tctgaatgta
    12241 tttattcgtt tttatttatc gttctttaga ggcattcccg cactagtcct gctgtttctg
    12301 ttatatttcg ggttacctta tcaattaagt gccttaacgg cttcagtcat ctgctttacg
    12361 attaccagca gtgcatttat tggtgaaatc tatcgaggat cactagcagg cgtttcttca
    12421 ggtcagtggg acgctggttt agcttctggt ttttctttct ttggggtatt gaaatatatc
    12481 gttttgccgc aagcttttcg aatctcagta ccggcactca gtaacgtagc tatggatcta
    12541 gtgaaaggta cttcgttagc agcaacaatc acagtgccag aaatatttca aaaagcaaag
    12601 atcataggcg gtcggacatt cgattatttt tctctctata ttttagttgc actgatttat
    12661 tgggtcattt gtatcgggat cgaaaaagtg caacagtatt tagaaaaacg ttctatccat
    12721 cagtcaatct gaaaaataga aatggtgcta gcgtggctgg caccatttct attttttgtt
    12781 tcttgtatat tctgcctagt attttaaaac catttcatgt aaaatgtaaa tcgaaaatat
    12841 gttcggaggt agaaaacatg aagaccccgc taaccatcga aatagaatcc tcgttatacc
    12901 aatattgcat cgagcaaggc ggcttggttg tagaagaagt cacgatgcct gatgaccaag
    12961 gaattgtaga tacgcttagc tgcttgacaa aatttgatgg aagccgagaa tggcgatgct
    13021 atgagctgaa agtgacaaaa gcagattttc gctctacagc caaactgtca tttgttggac
    13081 attataatta ttttgtttta ccaagtaaac tatatgaaga agtaaaaact gaaatcccta
    13141 caggaatagg tgttttagtg tatcgtccgt acacggttga agaggagatg cctgcctctg
    13201 gtacatttct gattgcgaaa aaagcagcaa agaaagagct tgctgtaccg gaagaagctt
    13261 tgacaaaccg cttcatggct tctttattca gggaagtaag aaaagcaaag caaatggagc
    13321 gaggcgtaaa atattttcca aaagatcaat tgtacaaaga attgaaaaga cgttcagaaa
    13381 atcaagatcc attttctaac gaacgatatt atgaacggtt tttagaggaa atagctagtc
    13441 aggcaatcaa tgagttgcaa gaagaagtta tcgcgcttca gcatgattat gaatatttga
    13501 ggcaacaaca gcagatccgt cggttgccta cagagccatt ggagtgaaaa atatgtacta
    13561 tccttattta agaggaaaac aatttgacct attagcatta aaagaagcac tgtcgcgagg
    13621 attgctctcc aataaaatcc aaccagtgat cgaaccagta cgtgattccg ctacgcttaa
    13681 aaatgtgatc gaattatttc aaaaaaagca ccatccaatc gctgtgatcc aaaatcccca
    13741 agtaggacaa ttcaaattat tcgatcagca tgtgcacagt tggacagtca aagaaaatag
    13801 ctctgtcgtc cctgcacaga tcttaacgcc agaaaatctc agtgaagtac tggattcacc
    13861 tccagcgttt cttgttttcg atggacagca ctacccaaaa gatacagagg tgtggaaaca
    13921 gttggcagga gttgattcga aatttttgat accagatact agtcgctttc gcatctggct
    13981 gcccgaaaac aaaattgtga tccgtgactc ttttcaaacg agaaagcatg ttgaaagtta
    14041 tgcagataaa acagatgact tttttagtga tgactatctt tttttccacg cagacggata
    14101 tattggattt tctgatttca cgatcgaagg tagccgttat ttcgataaag ggttccctag
    14161 ccgagcgctt gctatccatc taacgtatat tgatgcttac ggcaatatac gcgtgaaaca
    14221 ttttgtctct gacagtaacg actctgctaa agaccaagca ttgaagtttc ttgaagcagg
    14281 agataaaatg aaaaaatgga tcatgcgtca ccatcatcaa ctcttgatta cttcaggaat
    14341 gattgaatta ttggctcttt accagcaaca aaaattccct ggactgggag tgttgaaaaa
    14401 gtggtcactt atgcatcatt tggaacttat tagtcacctg ctagatcatc caaaagattg
    14461 gcttaaatat tattcaaaag ttcaggaact atatgaagtg atccaatgat tagataatgt
    14521 aaatgaaaaa ggaatagaaa agaggaaact ggatgtatat tcggcaaaca aggaaagaag
    14581 aagtagaaga agtggtagaa atcattaatg ccgcagcgga tttcattgct tcgcaaggct
    14641 ctcctcaatg gcaaaacggg cagaaaccaa caaaagaaac gattttgaac gatattgaaa
    14701 gaaaagaaag ctatgtatta atagtggaag atcaaattgc tggaacagct gcattagtct
    14761 caggcgtgga tcccgtttac acaaaaatag atggtagttg gaaagaatca actggtcctt
    14821 atttatcgat tcatcgtgtc gctttagcag aaagtactcg aggaaaaaaa ctagcaaaac
    14881 cgttcttaga acatttgttg acgattgcag ccagcaaaaa tatccaagat atccgtatcg
    14941 atacttatcc agctaatgaa ccgatggaaa agaccatcta ttctgctgga ttcacttatc
    15001 aagggatgat tgaatttcct tttgaacacg gcgaaagaaa agcatatcaa ataatcatca
    15061 attgactata aaatcatctg agcaaagata aagacattaa cactactgaa aaaaacaatc
    15121 atatgatagc caagaggttg taacagaagt ggccagcttc aaaaaacaag aaagaatttc
    15181 cgaaaatgct tctcaaattt ttgtggaatt ttagcgtatt ttacgaaata gctgctttct
    15241 gaaactccct tcatcgagag gtcgtgacat agcttttgtc acgatctctc ggcttatcct
    15301 acgagtatta ttcgatacat ttatatcaat aatccttttt ttattttatc tgcagcttct
    15361 tggaacgctc catcgtttga cacgactaaa agtgagggga tagctttcaa acgtttcaac
    15421 tgtgctaaat cttctacata aaccgccagt ctggctgaaa tctgttcaga tgaatctcca
    15481 cgttttttca ggcgcgattg caatgtttct ttagagatcg taataaagat aggacggact
    15541 accgtcccga aatactcata taaatgccaa aaaccatttg ctgtcaaaac ggcgtaacaa
    15601 tttcctactg tcagtttctt tttaatttct gctttactgg aaccatattt atgttgagca
    15661 tattggtccc actctgcaaa ctcatttccc ttgatcatct gattgaatgt attgtcagaa
    15721 acaaaataat aatcctcttg atctttttcc ccaggtcttg gtgatcgatt cgtgtaagta
    15781 atgatctttt gttccggctg aaaaatagct tctgcgagtg aagtttttcc agaaccagaa
    15841 ggaccgataa atacatagca ataattcatt gtcatttctc ctttgatgat tgaacaagtg
    15901 aaaaagcttt ggaaaatatt tccaaagctt ttaaaagtaa ctttgctttt ttgtatttta
    15961 taccagcaag ccagtcacaa tagcacttaa aaagctgact aaagtcgctg ttagcaaaat
    16021 tttcaatccg tgttttgcga caagatttcc tttttcttca tttaatccct tcatcgcacc
    16081 agaaatgatc ccgatagaag agaagttggc aaatgaaacg aggaagacag atataattgc
    16141 tgttgtacgc tcagtaaaat ggaaattacc atttgctaat tctgtcatag caacaaattc
    16201 atttgtaacc agtttagtcg ccatgatact accggcatcc actgcttctt tccatgggat
    16261 tccgctgatg aaggcaagag gggcaaagat gtaacctagg atctcttgga aagtgatgcc
    16321 gaatatcccg tggaagatag cattgatcat ggcaatcaaa gcaacaaaac cgataagcat
    16381 agcagcaacc gtgatcgcca catggaaacc atcaagaatg tattctccca acatttggaa
    16441 aaatgtctgt tctttgtttt cttcaatgat cagctcatct tctttttctt ctaatacata
    16501 gggatttaca attgaagcca gaatataacc gccaaataag ttcaaaacaa gggcagtgat
    16561 cacatattta gaatcgatca gtgccatata agatccgacg atcgacatag aaactgtaga
    16621 catagccgat acactcatgg tgaacaaacg tttctcagat aggaagggta attccttttt
    16681 tatggaaata aaaacttccg attgccctaa aatagcagaa gctactccat tataagattc
    16741 caatttgccc atcccgttga ttttacttaa tcctagacca acagctttca taaataatgg
    16801 aagaatcttg atatagcgta gaataccgat aatagcagaa ataaatacaa ttggcatcaa
    16861 tacggaaaga aagaaaggtg cacttccttt atttgctaca ccggcaaaaa caaagtcaac
    16921 accttgccca gcaaaagcaa gaagatgatc aaacattttt gccaatacag aaacaaccgc
    16981 tgtaccaaaa gttgtacgta ataatacgaa accaagaaca agctgtaaaa cgaacatcac
    17041 aattagtggt ttcaatttga ttgctttttt gttactgctg atcaaaaaag caagccctaa
    17101 tgtgacaaat aaaccaataa tacctaataa ataactcaaa taatcactcc tattccaaat
    17161 tccgttttat tttacctgtt ttgcaaataa aatactaatc tttagtgaaa ttttcattac
    17221 ttttttagag tgaaacgcat tcattttcca ttagaaaata aagcgaacaa aataaaaggt
    17281 ttaaaaagtg taacgtctta gtaaagtcta taaaaaaacg aaactatcga ttaagctaat
    17341 agtttcgttt tcaacattta taagtaaact ttttctattg gtaatataga ttttcggaag
    17401 ggtagacagg tgcgcaggta atatcgattc ctagtttttt caatgtttgt tcatcatttt
    17461 tattgaccat cacagtagag tgggcttgtg ctccctcaag ttcagctagt ttatcataag
    17521 caacttgagc gattggattc gttactgcac tgattgataa agcgatcaag acttcattga
    17581 tgcttagtgc atccaaagag gaatgaagtg tctttgattt caatccttgt attgtttcaa
    17641 gaactaatgg tgacaataaa ggaatgtcat ctgcgatatg ggctaaatgt tttactgcat
    17701 ttaagataac tgccgctgac gcatccatca atgaacttgt acgcccggta atgatgactt
    17761 catctggtaa ttcgattgca atcacagcag aaggatcttt tgtaggactt tgcgcttgaa
    17821 tcttatctgc atagtcatgg gcagggacta ccacgtttcg gtcttcagga cgtaaaccga
    17881 cttcttccat aatcagtttg attcgattaa ctgcttcttc gtcgatcaat ccttttttga
    17941 agtcgcattc tgtttggaaa taacgacgga tgatttcttg tttagaggct tcttggacaa
    18001 cggtatcatc cacgatccca aatcctacac gattcacacc catgtcagta ggagattgat
    18061 aaacagattc tttaccagta attttttcaa taatacgttt gattacagga aatgtctcaa
    18121 catcccgatt atagttcaca gctactttat tgtaggcatc aaagtggaag gaatcgatca
    18181 tattgacatc ttttagatcg actgtggctg cttcataagc aatgttcagc ggatgtttca
    18241 acggaacatt ccatactggg aaagtttcga acttggagta accagctgca tgtccttttt
    18301 ggctttcatg atacagttgg ttcaagcaag tagcgagctt tccacttcct gggccaggag
    18361 ctgtcactac aacgattggt ttagttgttt caatatactc attttttcca aaaccatctt
    18421 ctgagacgat tttttctacg ttgactggat agtcttcgat ttctgagtgt ttatagacct
    18481 tgatgtttcg tctttctagt ttattgataa agactttcgt ggcaggctgt ccactatagc
    18541 gagtgataac gacactgttg attaataagc cataaccacg taattcatcg attaaccgca
    18601 agatatccat atcataagta atcccataat caccacggat cttattacgt tcaatatctc
    18661 cagcatagac gcaaatcagt atttcagctt gatctttcaa tttttgcagc aatttgattt
    18721 tcgcatcttc ttcaaatcct ggaagtacac gtttcgcatg cttgtcgtca actaacttcc
    18781 caccaaattc taaatataat ttgtcatagt ggttcactcg ttccaaaata tacgctgact
    18841 gctcttcaat atatttttct gaatcaaaac caattttttt cactaggttc cctccttaag
    18901 tactaaagca ttttttcatt tttttgagga ttcgtcaact ggaagagcga aattcctcaa
    18961 aaaaacaaag aaaatggttt aaaccaactt gtctagatag ttattcattg gtatgatagt
    19021 ttttgataag gaaggtgcta catgaaaaaa agacagaaaa agaaaaacgc atataaacac
    19081 tatatccgtt cgatttttac cggttatgaa agaatgcttg aagatcccga attggaacaa
    19141 ttgacgttta cttatctaaa cgaagaaacg cagctaactc gtgatgagca tcagcgcatt
    19201 catttcacaa caagagatct gcctagtaaa taaaaaataa gataaaggtc atcggattcg
    19261 attctgtcct atcagatgga aagactagtt gatcgaaaaa aaggctgtac caaattttgg
    19321 tgcagcctgt tttatggagc attgcccgct cttttttata acaatttttt acagaaaatg
    19381 gtactattga agtattaata agtgaaaaac agatgagtaa gaggaggcaa gtagtatgaa
    19441 agagccagat acaaatgcaa attatgagcc agacgaacta gaggagacga acgcggatca
    19501 ggatacagga caagaatcgt cttcttttta cgatatcaaa gaaaaagcaa gcatgaatct
    19561 ttcccaatta ttagaagact tacaggcttt tgaaaaagct gtccgcgaag aagatatccc
    19621 tgagatctac cgtttataca atggccgatt gaatgaagaa ttaaaaagaa gctcaaatga
    19681 aaatcacgag atcgatcaat tgctgatgcg taagatccat gatcgttttt tacaggaatt
    19741 tccttttatg gaacaagtag aaaacatctc gccaacgatg agctattaca aaatcggcac
    19801 ttattaccat gatcggccca cggtcggtat tgatgcaagt ctgccagaaa tttttgtttt
    19861 gcctaagatc gatgaagagt gggagaaata ttcccaaccg gagtcaacgg actatgacaa
    19921 aaaaatcaat gagctagacg caaaggtgat cactgctcag acagaaattg aacgtttaga
    19981 tgaacaaata aaagaaatca atcgacaaat gactgatttg aacgacaata aaggattctt
    20041 gaatcgtaaa aaaatcgagg aagaaattca agaattagag aagaagaagc aagtactatc
    20101 taatgaaaaa ttaggctggc ttccttatat tgagacgcct gaaacaatcc agcagcaaaa
    20161 agaagcattg aagcaagaag caagagcaga ccagcttcgt gcagccatcg tagaaaaaga
    20221 acaaaggcaa atcagacgct atttcggtag caaagaggga ttcggtcaag caatccatga
    20281 attcttgatg aattatcttg gcaatgagaa tcctaataat caatctaatg aaggaggttc
    20341 cgagtatgaa tgaacacgat gaagcagaat ggacaaaaaa tgaagaacaa acagaagatc
    20401 atgaagaaac gaacacttca ttggaagaac aaacaagtaa tcctctggat gacacagaag
    20461 aaatccctat gctagaggaa acaaaaatac atgaaaaaga aaaagtaact ccgacgaata
    20521 cgaatactgc agaaaacacc catgcttacg aaataactga aaatcccggg tatttagaag
    20581 gaaaagagca agatcatcaa gatatcgtca gtttgattga aacagcggaa gcagaattag
    20641 cggatttacg tatgagaaaa gatttgatcg aatcagattt tccacaatta ttagaatcat
    20701 atgatcgttt tatattgaat gaagaaccaa tctccacgat cggaaagaca agcctgatcg
    20761 aatacttcac gtatgaatta ctgaaaccgt tgaatgatat tggtctggaa cggtcaaatt
    20821 cagcttatga cacatggaaa acaagacatt tttcgatcaa ttatacgttg aatggacaaa
    20881 acgaattagt cttttccttt gtattaccga ccatcgatca acgataccaa gtggaatcga
    20941 tgagtctcct aaacgtatca ccagagacaa tggaaatcaa tgtccaagat gaccgggtgc
    21001 tttctttgat ccgacactgg tcagtggatc gagtattctc tgcagggcag atcacgatat
    21061 tcaatcacaa attgaatcaa ttattagccc atgcaagaac acttggtttt accgttaatc
    21121 agacattact agacaatacg aagccacttc gtttgaatct tcagagcgaa tttgaattga
    21181 cgaatgaagt attggatgat atttttatcg tggcaatgaa aaatccatcc tacgatatgg
    21241 aaaagatcaa cgaaactact tatcaagtac gattagataa aggacagtct ttaacgataa
    21301 caacaaacga aaaagatcaa acagtcttgt cgatctcatc tggagactac ccacgatctg
    21361 tcattgattt ctttatcaat tacgaattct tggtcccatt aatggtgaga aagtcctgat
    21421 tggataagtt ggattaccag atgtcattca gataattctg ttccaaagga cttgcggcag
    21481 ttatccagaa taattgtcac aagtcttttc tgtatgtact aaatggacgt gaataacttg
    21541 ctgatgaagg agggatttca ttaggaatgt ccacaaataa aatagcacag cttagcttat
    21601 tgtctgctgc ttgtgtagca ggtaggatcg cttttcaatt tattccgaat ttccagccaa
    21661 tgactgctat ttttttattt atagtacttt acttaagctt aaaagatgcg ttaattgtca
    21721 tgagtctttc tattgcgatc agcagttttt attttggtgt tggaccatgg gtcatcggcc
    21781 aatggatcag ctatactgtt gttctgagtt tgttttcaat cgtctgcttg agaaaaacag
    21841 tgcaaaggaa tctatggtta aagggagtct gtttttttct ggcgggcatg gtttacggaa
    21901 taggtatgac cgtttttgat acgctgttat atcgtttgcc tcaaccttgg atctattacc
    21961 ttcaaggggt gtcgtttgat ttgatgcaca gtataggcaa tgttgtgttc tttcttgttt
    22021 ttctgccagt cgtcaaacgt ttttacaatc attcaggagg aaaaaatgaa aaaaataatt
    22081 tatagtttgc ttgcaatgag tgtcagtata ctcattttga caagttgcag tacaacctat
    22141 acacaatcgg cttccaacca aaaggaggaa gtaaaaacat cgcaaaaagc aacaatcacg
    22201 ttacaagaaa atggtcaaaa cttttcggaa aaagaagtgg tcttcaaaaa aggcgatgat
    22261 ttactgagta ttttgaaacg aaattttgaa gtcaaagaag ataacggctt catcacttcg
    22321 cttgaagggc actcacagga tgagaaaaat caactctatt ggatgtttac tgtggacggg
    22381 aaaagtgcta ctactggtgc caaagagatc aaattgaatg atggacaatc ggtcattttc
    22441 aatttgtcta aactataagt ttcataaaaa actgttaaag ggcgaatatt gccataattc
    22501 agtgagttca gttgatgaaa gaaggaaaat aacatggaaa attttaaaaa aagcggaatg
    22561 ttttccgtaa ttgctgctct aggggctaat attctagttg ctatcagcaa atttgtcggc
    22621 tatgcaatca gtggcagtgc cgccatgcta aacgaaagca tccacagtat cgttgattgc
    22681 agcaatcaga ttttgctgtt attcggtgat aaacaagcca ataaaggaca gagcgagttg
    22741 catcaatttg gtgaaggacg agcaaaatac tttttcagta caatcgtcgc aatgatgctt
    22801 ttcttcggcg gtggggcact tggggtgatg gaagcttttg aaaagttgac gcatccatcc
    22861 catgaagtgg gtaatccgat cattgttata gcaatactgt tatttggttt ggtaatcgaa
    22921 ggaagttcgt tacgcgtggc aatgaaagaa atcaaagaat tgaatactga aaaactttcc
    22981 acgatgcgct ttttgcgcga aagtcgacac agtgaaatac tgattatttt tacggaagac
    23041 ttgtgtgctg taatcggttt ggtcttggct ttagcaggaa caatattgac ttatgcaacc
    23101 gggattgctg catttgatgc gatcagcgga ttgctgatcg gtttcttgct gatgggagcc
    23161 gccattttcc tagcaaaaga attttatagt ctgatcatcg gtgaaagcgt gacagcaagc
    23221 gatttatcaa aaatcaagtt agcttttgaa cgtcctgaga tcagtcggct gatcgatata
    23281 aaaacagttc atttaagtcc aacggagatt ttagttgcag caaaagcaga tatcgttggt
    23341 aacaaagaga atgaagctta ctcggtcatc aatgatattg aaaaaaatat gcgtcagtct
    23401 cttcctgaca agaagatgta tatatacatt gaaacagata aatatgaccc aaattattct
    23461 cgtaaataaa acgtttattc atgaattgaa gcaaaaatga gaaaaatata atcttgtttt
    23521 taaataaaac ggctacaatt tagcgaattt atgttacaat gagtccgctg tttatttgtt
    23581 gtaattaaag gagatatttt aatgaacaaa aagaaaagtg cgccatggct tcaagctttg
    23641 catgcagtga tgccactttg tataagctat ataccagtag gattagcttg cggagtattg
    23701 ttgcaaaaag ttggatttga tcctctctta tcaggactgt tatcgattct ggtcttttcc
    23761 ggtggcgcgc agtttttagt tgcatctatg cttacgacgc aagcatcatt tgcaacgaca
    23821 ttattgatgg ttttcttttt agaattacgc tatacattat taggagcaag tcttgccggt
    23881 tttatgaaaa aagaaaagcg gacgtttttg gctgtctttt ctcaatcact gaatgatgaa
    23941 aactatgcgg tgaactactt aaagttttca actgaccctt cctggaataa aagaaaggct
    24001 ttatatgtca attggttctc aatgactgca tgggccggta gcaatatgat cggtaatttt
    24061 ttcggctcgg tgattcgagt agatgctgac cttgtccatt ttgctttgac ggccatgttc
    24121 attttcatgt ttatcatgca gatgaaaagc gcactactga tctttacagg tttattctca
    24181 ggcgtattag gggtcgtatt catggtatta ttccaaaata ctttcggatt gattgcagca
    24241 acagtcattg cttcatttgc cggatttatg ctggagaagg cattcaaaaa agaaaaagct
    24301 aagaaactaa gagaccgcgg taaagatgtc aatgagccgc tcttcaactt cccggaacag
    24361 gaggccaatc accatgacta aactatattt gctactttta gttgcttgtc tgttcatcgt
    24421 ggcatatatc ccgcgcttgt tcccgatgct ttattttact catcgaaaag tgccatcctg
    24481 gttcagtgac tggatgaaat atgtaccggt ggcattattt gcggcgttag cttttaaaga
    24541 tgttttcatt actcatgaac atctggatat tgcttggaat atcaaaatag ctgcaatgat
    24601 tctagtagca ggcgtggctt acaaaacacg ttcgatggcg ttgtcagttt tgacaggact
    24661 tgcgtctgtc tttttgctgt ctatgcttta aactgtttta ttttattaaa aacagagatt
    24721 gcgacataag ttgttcaaac tgatctatcc gaactgcatt tgcaacagtc aattctggat
    24781 gcaagtttat tcgtagggat aaaaaagatt agtgttggct tatgatgcaa gctctttttt
    24841 attttaggaa ttagagaggg atttgtcctt ctatgatttt ttgctataat agggaagatg
    24901 tttattttat gaaagaatag ttgagggaat ttaaatgaat aaaaaattga tgcaaggaac
    24961 attttggcta acctttgcta atcttctatg taaagtttta ggggtagtct atttgattcc
    25021 ctggctaagt atgatgggga acaatcaaga tggtatgctt gctacgacat tgtataatgt
    25081 tggctatctt ccttatggat tgttcctgat gcttggtaca gtcggatttc caaatgcgat
    25141 tgcaaaaaaa gtagctgtag ccacaaaaga aggagataac gctgcttgtc ggctgatttt
    25201 tagaagtacg atcaatatca tgtttgtcat cggtatcgcc tctgctgcac tgatgtattt
    25261 atttgctcct ttactagccg gaatcagtcc gattgctaac gttaataatg ggatactagc
    25321 tatcagaagt ttatgcccat ctttgattgc tatcccaatt cttagtgcaa tgagaggata
    25381 tttccaagga aaaaatgatc tgcggcctta tggaacttct ttgattatcg aacaggctgt
    25441 gcgagtgatc gttatcttgg ctggtactta ttacttgcgc gtattgactg acggaaccat
    25501 attagaagct gtattgatca gtacagtcgc ttctttcttc ggtgggttag ctgcgattat
    25561 ccatatgtat atcgtcggac gcagaaaaga cttcttcgaa cttaaagatt ttttgatttc
    25621 cagccgttat ttcgaaagag aaaaccgatc tgcctctgtc tctatcattc gggaaacact
    25681 accgtttatt tttgtcggct cagtcattac tatcgttcaa atgatcgacc aagtaacgat
    25741 gaaaccactg cttcactttt tccgtccaga gatcgcagat cagcaactgg aattcttatt
    25801 cagccgtgct tctgtcaatc caaataaatt gacattgatc ctgatctcca tggtaggaac
    25861 agtagctata agcagcttgc caatattaag cacgatgaag aaaaaagatc ggatacaaat
    25921 cgaaaaaaca gtaggagata gtttttcgat tgcgttattg attcttcttc cttcattgac
    25981 tggaatgtct ttattagctg gaccactata cacattgttt tttggctatg atcctgaaag
    26041 tgtcggatat ttccaaatgg ctttgctagc ttctttgttt ttctctttat tcacgatcct
    26101 atctacgatg cttcaatctt tgaatcacca tcgttttgcg atccgattaa ctgtagaagc
    26161 aattatcctg aaagtcatct tccaagtaat cggattagga ttagttggcg gatacgggat
    26221 gagcttatca aatggcttag cattcggtat tgtctttgtt cgcggctatc actttatgtg
    26281 caaagaatac cgaatagcac ctttagcaaa gatcagcaat tttttcttaa aaactttccg
    26341 ctccaccctg attatgcttg cagtatgttt tgccgttttc tttttgctga atcagcgatt
    26401 gtcaatggaa tcaaaaagct atgcaatgat ttattgtgtg gctgtaggaa gtattggcgg
    26461 cttagtattt ttattttctc aattcggaaa aaatgggttt cgaatgttga aaaatgttaa
    26521 acatcatagc agaagcaaag aataggttaa ctaatcgttt tttgctgtca cttgaaaaac
    26581 ggcaactcgt atgattacgc ttgtcgtttt tctattttcc aattttagtt tcatatctaa
    26641 tcttacttat gatatagtta taaggtctgg aaaaattctt tgaaacgagg aaattaaaca
    26701 catgaaactt gcagtagtta cagatagttc ggcatatctg ccggaacgta tcaagaacca
    26761 tcccgactta ttcgttattc caatcccttt gattatggat gggaaaattt ataacgaagg
    26821 aatcgatatc gaagcggatg aatattatgg acttttaaaa aacagtaaag attttcctac
    26881 tacttcacaa cctgtcgtag gggaagtttt gggtttatat gaggaattga aagagaaagg
    26941 gtatgacacg attttaagta tccatctttc ttcagggatt tctggctttg tcaacacatt
    27001 atttgcgatg aaagatgaca tcagcggagt aacggtcatt ccttatgact ctaagatcac
    27061 tagtatgccg atgggacaca tggtagaagc tgccttagac ttaaatgatg aaggtcagtc
    27121 ccttgatgaa atacttgcac atatcgatcg aatccgagat aatacctacg cttatctgat
    27181 tgtagatgat ttgaacaatt tagtacgagg aggacgtttg acaaatggtg cagcgctgat
    27241 tggcagctta ttaaaaatca aaccgatcct gacattttct gaagggaaaa ttgttttatt
    27301 cgaaaaaatc cgttcaagta aaaaagcatt tgctcgagct gaagaaatta ttggtagaag
    27361 aaataacgaa atacaaccgc cagtcaaact ttacgtgata catgcaaata atttagaagt
    27421 agcgattgcg gagaaagaga aattgcagaa aaaatatcca aatgcagaaa tcgaaattgg
    27481 tcatttcgga ccagtcattg gtacacattt aggtgaaaaa gccatcggca ttgccatttc
    27541 agcgcaataa ttccttttat tcaaaaaaca taccaaaaat attccaacat catcttggac
    27601 tttctacatg ttactaatga gcgtgtaaaa gaacgagatg gtgtttttat ttctatcaat
    27661 gaagctcttt ttctttgcaa tcctcacttc aatcctctat aatgtcgcat atagtttagt
    27721 aaatgagtaa actactaatt tacttattta ctaaaaataa aaggatgagg agttaaaata
    27781 atggaaatac caacaaattt aaaacataaa ccagtaatcg ttgtagaaga ctatgataaa
    27841 gttgatggaa gaaatgcatt aaatacggat gcaaaagggc tttcactggg ccttgctcag
    27901 tggaatgatc gtggaaaagt cgatatctca ggcaaggtat ggcgacatac tggagaaaaa
    27961 tggtcacgac agtcggaaga attaccaatc acacgtattt tagatttagc gatcttagct
    28021 gcacaaggaa gtttatactt ccaagatgct tatcgttatg aaaaatttta tgatccagaa
    28081 aatccagtcg tagacatcat tggattacaa gggaaccgaa tgacggtaga agtagatacc
    28141 aaaaatccaa agatcgatga agacatcatt ttgtattacg atacactaca aaaagacgga
    28201 gaactgattg gggaacgatt ccgtatttta aaacgtcttt tagatgagct aggatattaa
    28261 tagacagaga tgatcaaaag gggaagagaa gcatggagaa tttctcacta acagaaaacc
    28321 gaaccttttc acggctagaa aagcaaatga tctggaagaa accgaaaagc catcggacaa
    28381 gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa ttggcaagat cctgaaatga
    28441 aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg gaagacacaa ttagccaaag
    28501 cactatctgc tgatctgcaa cttccctata caaaagtgac ctgttttgca gatatggaca
    28561 aatcagatgt tttcggagcc ttattacctg tagtagattc taataaggaa caagaccaag
    28621 aattactaga agctatttac caaacagata cacttgaagc tgtactgaca ctgatccaga
    28681 accattacgg cattgatcct ttttctgcta aagaaaaatt agcagagcta gttcaaagga
    28741 tcgaagctga ttatccagat gcaatccatt acagctatta cccttctgaa atcgttcgag
    28801 cgattgaaaa aggatattta ttagagattc aagagccgac tgtcatccga gatgcctctg
    28861 ttcttgtggc acttaactca gcgctcgaac caaatgggat gttgaattta ccgacaggaa
    28921 tcattcgtcg ccatccagat tgcgtcattg tgatcactac aaatcgaaat taccaaggaa
    28981 atcgcccatt aaatgaatct ttaagagatc ggatgcaaca tgcggaaaaa atggatcttc
    29041 ctgccttaga agtcatggca gaacgtgcga tggcgaaaac tatgatccaa aaacaagaac
    29101 ttctcttaaa gatggcagag attatccgtt tgttggatga aactgcaaaa gctaacgcca
    29161 tcaaaggtgt agcaggaatg cgttcttatt tttattgggc aaatacctat aagcaaggac
    29221 aagatttact ccagtcgatt tatccaaaag ttttgtataa attatccact gattcagatg
    29281 aattggcaat tcttgaacaa gctttgtctg acagtggatt gctactggaa cttcaggaca
    29341 tattgagaaa agaaaaatgg ggtcatcctc aatctgagca tacaaaagga agaaccatta
    29401 gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc tgaagaaatt ctgcgcttag
    29461 atgaaaagaa atcgttgtct gctgaagaac caaatgaggg agaagagaaa caagaagaaa
    29521 caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga acgcagtagc gatatcgaag
    29581 gaaaaaatca aaaagaacaa caaacaggag atgattctca agaaatgtct gcttctgaca
    29641 tggaagcaca tgataaggaa atcaagaagc aactgaataa agaagcgcga caaatcatga
    29701 aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg accagctttc caaggaaaaa
    29761 cgccggaagc acgtgagcta cacatgcaag taatgccaat agtagaatca cttagccggc
    29821 aaattttaga gttactggat aatgaacaaa cagagacgta ccaaaaagga aaatacgaag
    29881 gtcagcggtt caatgcaagc aaagtagcat atggtgattt gagaaatttt gacaaaaaga
    29941 acccgccaca tgagcaacct tctttagcgg tagctgtacg gatcgatgaa tctgggtcaa
    30001 tgatacggga tgatcggatc gaagcggcaa aaaaagcagc gattgccatt gccgaatttt
    30061 ctaagaaagt cgatatccca ttgttgattt acggagatac agcagaccgt tcgtcaagag
    30121 aaaagacttc tttgttttct tataaagaat ttgaagacgg atttcaatgg ttaaatgaaa
    30181 aattagtcac tatgaaacca aggcaaaaca atcgtgatgg tgctgcactt cgtttagcag
    30241 cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt gcttaatatt agtgacggtc
    30301 agccaaaagc gttgccggat tatacaggca aaaaagcaaa agaagatatc cagtctgttt
    30361 tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc agctattggc caagacaaag
    30421 aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga tattactgac ttgaatgaat
    30481 ttcccaagca aatgattcaa ctgattgccc gctatctatg ataaaataaa gctcaacgaa
    30541 cgcacttagc agattcgttg agcttttcgt ttctagttac cggtaacaag gatacttcta
    30601 aaatagaatc ggttataatc aagagggacc gttaaaggag tgaaacgata tgatacaaga
    30661 acaaaaatgg tggaaagaag cagtaggcta tcaaatctat cctgctagtt tcaaagatag
    30721 caatggcgat ggtcaaggag acatcaatgg gatccgtgaa aaacttaatt atttgaaaga
    30781 tcttggtatc ggctttatct ggatcaatcc gatctatcaa tctccatttg tcgataatgg
    30841 ttatgacgta agtgactatc aagatataga agagagattc ggaacgatgg aagattttga
    30901 tttattgcta aaagaagcac atgaattagg gatcaaagtg attatggatc ttgtcatcaa
    30961 ccatagttcg gatcagcatc aatggtttga agaatcgaaa aaatctaaag aaagcccgta
    31021 tcgcgattat tacatttggg tcgacggggt agatggcaaa gaaccgaata attggacatc
    31081 gattttcggc ggttctgctt gggagtattc tcatgaaact ggacagtatt atctgcatgt
    31141 ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag aaattgaaag aagaactatt
    31201 caatatgatc cgatggtggt tggataaagg gatagatgga tttcgtttag atgcgatttc
    31261 tcatgtaaaa aaagatgaat attcagtaaa agccacagag aaccctttct caccctttca
    31321 aaatgtctca ggaatcgaag aacatttgac cgatttgaaa catgtttttg aagagtacga
    31381 tatcatgact gtcggagaag caagtggtgt cactgcagaa gaaggaccac aatgggtagg
    31441 gaaagacggt tatttcgata tgatattcga atttgatcat atccatatct ggcagcaaga
    31501 aaaagaaggg caattagatg tccttaaatt gaaacttgca ttaagcgctt ggcaaacttc
    31561 attggatggt atcggatgga acgctttata tatggaaaac catgatgttc ctcgtgccgt
    31621 ttcagtgttt ggagatactc gtcccgattt ttgggctatg tctgcaaaag caatcgctat
    31681 gatgtatttc tttttgcaag gtacgccatt tatttatcaa ggacaggaaa tcggaatgac
    31741 gaatatgccc tttgaatcga tcgatcaagt tgatgcagta gattcgaaaa gactttataa
    31801 aaggctgctt gcggaaggta agacaagaga agaagcattg gatatcattc gtgaaacaac
    31861 gagagacaac agcaggacac ccatgcaatg gacttcggaa caatatgctg gtttttctac
    31921 acatgaacct tggctgatca ctaatccaaa taccaaaacg atcaatgtag aacaacaaga
    31981 atatgaacca gagtcggtcc tgcaattcta taagaacatg atccgcatta gacaaaccaa
    32041 taaaggtctg atatacggtt cttataaaga atatcttcat gaacatccac aactttatgt
    32101 ctatgaaaga tatttagaag atgaacaata tcttattatg gtaaacttaa cagaatcact
    32161 tgctgactat gaacttccaa aagaagcaga tcagtcctgg acattactct tgtcaaattc
    32221 ctctagcgga gaatttgaag ctaaaggcat actagcacct tatgaagctc ggttatataa
    32281 aacaaacaag taaaacttcc tcgttaaagt tcattagttg gactttgcga ggaagttttt
    32341 cagattgctc tgtcaattac tcatttcaat aattcgctct aggaccaccg atatatttag
    32401 gacgagagcg aagggctgtc acgtgagctt gtccaagggt tttcaaacga ggtctgacag
    32461 gtacttggac atgtttgata tgcataccga ttgatgtgtc accgatatcg ataccggctt
    32521 gtccaactac gtgttccact tcgacaggat cattgaactg gtcaaaagca gcaacagaac
    32581 aagcacctcc agcatgtagt gcaggaacaa cagagacaat ttcgaaattt tttgcttctg
    32641 ccactgttcg ttctactact aatgctcggt tcaaatgttc acaaccttgg acagctagcg
    32701 caatttcttt aggatccaac aactctttta acgtacggat gatccattga ccaacttccg
    32761 cactggaatt ttttccaata tgtcctccaa cgacttcgct tgtgctgcat cctaaaacaa
    32821 aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc attaactatt tcagtcaatt
    32881 ggtcttttaa ttctttttct gacaacttca ttttctaaaa ccttcttttt caattaatat
    32941 tgcttccgtg cttgaatacg ttgaagactt gcagttatag gaaaggcaag tcccattcca
    33001 atcagatttt ggaagatgtt tcctggtata gaagccactc cagccggcca atcatataaa
    33061 atccaaccgg cgataccata gccgatgatc ataactaagc ttcctaataa aagccctaat
    33121 ccccacatac gagtagaaga tttgctgaaa gaaccagcaa catatccttg taacccgtgg
    33181 atcactaatg aaaaaactgc ccactgcgga tatcctgaaa tcaaatcgat caatagacca
    33241 ctggaagcac caacaactaa tccacccgtt ggaccataaa gaaaagcagc ggtatagatc
    33301 ccggcctcgc ataatgtcac aaatccattc gtagccggta cagggatcaa aattgttaat
    33361 gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg ttaattgctt agttttcatt
    33421 tctcattcct cctttttgaa taccgccaga ccagacactt ccggtatgat cgttcatcag
    33481 cacgccatta gaaatacaat gggcaacaaa ttttttactt tctgctactg ctttggtaaa
    33541 atctgcccct aacacaagat tcgctgttat tgcagaagaa aaggcacaac cggccccgtt
    33601 tactgttgta gtgtcgattt ttttatgaga aaatacctca taatgatcaa ttgaatagag
    33661 aagatccaca gcttcatctc cagccatcct tgtgccgcct ttgatcacaa tgttttttgt
    33721 tcctgaagca gtgagttctt tagcgagatg gatcatttct gttaacgaag agggaagagc
    33781 ttgaccagaa agtaaagcag cttctgccaa attaggcgta atcatcgtaa caagtggaaa
    33841 aagttcttcg atgagctgcg aaatatacaa ttgattgatc acagcttcag tttctttgaa
    33901 tgccaaaaca gggtctaaaa tgattgggat attttgtttt gtttttatga agtctcgaac
    33961 aaccatgatt gcttcacttg aattcaataa cccaattttg ataccatcta atgaagtcat
    34021 tttatcaatc gaaactaact gtcgtttaag caattccaaa gatatagatt ccagcataaa
    34081 tcgctcatta atatctagtg cccctataga agtcaaggcg cttagtccga atagaccaaa
    34141 attctcaaaa gtttttaaat ctgtttggat tcctccacca gcaaatggat cagaaccgcc
    34201 aattgtcaag attcttttca taacattttc ctccatgaat aagagtataa cgaaattttc
    34261 aaaaaagaaa aacagccaat tg
//
LOCUS       DAZUMM010000024        33269 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000024 DAZUMM010000000
VERSION     DAZUMM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.024"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..233)
                     /gene="rpmB"
                     /locus_tag="ACG8E6_001077"
     CDS             complement(45..233)
                     /gene="rpmB"
                     /locus_tag="ACG8E6_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFS5061515.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            503..865
                     /locus_tag="ACG8E6_001078"
     CDS             503..865
                     /locus_tag="ACG8E6_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5061516.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            917..2599
                     /locus_tag="ACG8E6_001079"
     CDS             917..2599
                     /locus_tag="ACG8E6_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFS5061517.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2716..4752
                     /gene="recG"
                     /locus_tag="ACG8E6_001080"
     CDS             2716..4752
                     /gene="recG"
                     /locus_tag="ACG8E6_001080"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFS5061518.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4801..5802
                     /gene="plsX"
                     /locus_tag="ACG8E6_001081"
     CDS             4801..5802
                     /gene="plsX"
                     /locus_tag="ACG8E6_001081"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFS5061519.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5924..6166
                     /gene="acpP"
                     /locus_tag="ACG8E6_001082"
     CDS             5924..6166
                     /gene="acpP"
                     /locus_tag="ACG8E6_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS5061520.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6434..7438
                     /locus_tag="ACG8E6_001083"
     CDS             6434..7438
                     /locus_tag="ACG8E6_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5061521.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7439..8383
                     /locus_tag="ACG8E6_001084"
     CDS             7439..8383
                     /locus_tag="ACG8E6_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5061522.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8383..9345
                     /gene="opp4B"
                     /locus_tag="ACG8E6_001085"
     CDS             8383..9345
                     /gene="opp4B"
                     /locus_tag="ACG8E6_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS5061523.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9372..10286
                     /locus_tag="ACG8E6_001086"
     CDS             9372..10286
                     /locus_tag="ACG8E6_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5061524.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10312..12093
                     /locus_tag="ACG8E6_001087"
     CDS             10312..12093
                     /locus_tag="ACG8E6_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5061525.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12441..13127
                     /gene="rnc"
                     /locus_tag="ACG8E6_001088"
     CDS             12441..13127
                     /gene="rnc"
                     /locus_tag="ACG8E6_001088"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFS5061526.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13147..16728
                     /gene="smc"
                     /locus_tag="ACG8E6_001089"
     CDS             13147..16728
                     /gene="smc"
                     /locus_tag="ACG8E6_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFS5061527.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16737..17546
                     /locus_tag="ACG8E6_001090"
     CDS             16737..17546
                     /locus_tag="ACG8E6_001090"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5061528.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17557..18555
                     /gene="ftsY"
                     /locus_tag="ACG8E6_001091"
     CDS             17557..18555
                     /gene="ftsY"
                     /locus_tag="ACG8E6_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFS5061529.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18742..21009
                     /gene="gshAB"
                     /locus_tag="ACG8E6_001092"
     CDS             18742..21009
                     /gene="gshAB"
                     /locus_tag="ACG8E6_001092"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFS5061530.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21098..21550)
                     /locus_tag="ACG8E6_001093"
     CDS             complement(21098..21550)
                     /locus_tag="ACG8E6_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFS5061531.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21700..22650
                     /locus_tag="ACG8E6_001094"
     CDS             21700..22650
                     /locus_tag="ACG8E6_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS5061532.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22643..23602
                     /locus_tag="ACG8E6_001095"
     CDS             22643..23602
                     /locus_tag="ACG8E6_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS5061533.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23599..24354
                     /locus_tag="ACG8E6_001096"
     CDS             23599..24354
                     /locus_tag="ACG8E6_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5061534.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24377..25330
                     /locus_tag="ACG8E6_001097"
     CDS             24377..25330
                     /locus_tag="ACG8E6_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5061535.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(25974..26270)
                     /locus_tag="ACG8E6_001098"
     CDS             complement(25974..26270)
                     /locus_tag="ACG8E6_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFS5061536.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26625..26981
                     /locus_tag="ACG8E6_001099"
     CDS             26625..26981
                     /locus_tag="ACG8E6_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS5061537.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            26991..27890
                     /locus_tag="ACG8E6_001100"
     CDS             26991..27890
                     /locus_tag="ACG8E6_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5061538.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            28410..29138
                     /locus_tag="ACG8E6_001101"
     CDS             28410..29138
                     /locus_tag="ACG8E6_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS5061539.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            29283..31310
                     /locus_tag="ACG8E6_001102"
     CDS             29283..31310
                     /locus_tag="ACG8E6_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFS5061540.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            31418..32515
                     /locus_tag="ACG8E6_001103"
     CDS             31418..32515
                     /locus_tag="ACG8E6_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS5061541.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            32822..>33269
                     /locus_tag="ACG8E6_001104"
     CDS             32822..>33269
                     /locus_tag="ACG8E6_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5061542.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT        11143 a         5759 c         7124 g         9243 t
ORIGIN      
        1 aataaaaaac cgttcactat ctgaacggtc caagtatcat tcaattaaac gcgttcaact
       61 tttcctgatt tcaaagcacg agttgacacc caaacttttt taggttttcc gtcgaccata
      121 acgcgaactt tttgtaagtt aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa
      181 cggttgtttc cactttttgt cttgcgacct gtaaagtaac aaacttttgc catggtaatt
      241 tcctcctttg ccttgatttg gagcaataga ataattcagc tcataccaga ctaacatatc
      301 atatagaatg ggaatttgca agtacatttt catagataca attgacaatt gagaagaata
      361 attgtaaatt aaacaaggtt atacctgaaa ttttgcaagg tttttgagta ctttgggtaa
      421 ctgcctttta tttcaagttg cgctatgata aaatatgata gaagaaaaat gggagttttt
      481 tacccgttaa ggaggctcat tcatggctgt aaaaattcaa acgtcagctg gaaccattga
      541 aattaccaat gatgtaattg caacagttgt tggtggcgcc gcgacagatg tgttcggtgt
      601 tgtaggaatg gccagcaaaa atcaaattaa agataatatt actgagattt tgcgtaaaga
      661 aaattactca agaggagtag ttgtccgcca agaagataat ggtattgctg tagatgttta
      721 tacgattatc agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt
      781 gaaatacaat ctagaggcat tattaggcgt aactgccaat tcagttaatg tctttgtaca
      841 aggtgttcgt gtactgccag actaggcagg agactggctg gtaactcagc agtcgatctg
      901 aaggaggatt atctaggtga gtgtaacaga aataagtgca agccagttcc aggaaatggt
      961 ccaggcaggt gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt
     1021 ccctgtgcca gatggcgata cgggaacaaa tatgaatcta tctatgacta gtggagctaa
     1081 ggcagtagta gattcaacct cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg
     1141 attgctgatg ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt ttcgtggatt
     1201 ttctaagcaa attccagatg ttgtctcatt aaatgcttct gatcttgctg cagccttcac
     1261 tcatggagtt gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa cgattttgac
     1321 agtagctaga gaagcagcga aagctggaga aaagaaagca aaaagtactg atgatgtcgt
     1381 tgaagtaatg acagctgttg taaaaggagc aaagcgtgct ttagctaaaa ctccagactt
     1441 gcttccagtc ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt
     1501 ttatgagggc tttttaagtg cattgaatgg agaattccaa gcggatgaaa catatgaacc
     1561 ttctccagca gaaatggatg atatggtcaa tgcagaacat catcgcagtg tccaaggtca
     1621 aatggcgaca gaagatatcc attttggtta ctgtacagaa atcatggtaa aaatcggaga
     1681 aggacctaca gtcgatagta cattcgatta cgaagaattc cgtaactatt tagatggtat
     1741 aggagattct ttacttgtgg tcaatgatga cgagatcatc aaagtccacg tgcatacaga
     1801 aaacccagga gaagtcttga attatggaca aaaattcggt tcattagtca aaatcaaagt
     1861 cgacaacatg cgcttgcagc atgagacgat tttggaacac gatgaagaag tgaacgagtt
     1921 tgcgaaaaaa gaaataccac gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg
     1981 cgtccaagag ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg gacaaacaat
     2041 gaatccaagt acggaagaca ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat
     2101 tgtcttgcca aacaataaga acatcttcat ggcagctgat caagcagcag aagtagcaga
     2161 tatcccagta gcagttgttc cttcaagaac gatttcccaa ggattgacag ctatgctttc
     2221 attcaatgat caagcttctt tagaagaaaa caaagaaacg atgacagaaa tgttgtctag
     2281 cgttgtgagc ggtcaagtca ctcacgcgat cagagatacc tcaattgacg gtgtagagat
     2341 caaagaagga gactttttgg gcatgatcga tggaaaaatc ctgatctcta acccagatat
     2401 cacagcaaca gcgttaagta cactagaaca aatgatcaat gaggacacag agatcgtaac
     2461 gatcttggca ggtgatgaag gaacagttga acaagcaaat gaatttgctg agaaactaac
     2521 agaaaaacat cctgatttgg aaatcgagat ccaccaagga gatcagcctg tctatcctta
     2581 tctattttct gctgaataat ttctgtgtca gaatatcttt ctaacacagt attcattcat
     2641 aaaaggcctt cggttagtga tcaggtatga tcgctgacca agtccttttt ttgttaaagg
     2701 agggaggaaa aatttatgcg ttctttagat gatccaatca ctatgctggc aggagtcgga
     2761 gaaaaaagag cagctagtct tgccagcttg ggcatccaaa cgattgaaga cttactgact
     2821 tattatcctt ttcgctacga agatattcag gaaagaaatc taaacgagat ccaggatcaa
     2881 gaaaaggtta ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg atttggttat
     2941 aaaaaaagcc gattgcaatt tcgtatgatg caagaacatg atgtgttcaa cgtttctttt
     3001 tttaatcaac cttatctaaa agaaaaagtc attttatcgg aggaaatcgc cgtatacggt
     3061 aaatgggatg caaagcgtaa agccttgaac gggatgaaga ttttaggttc tcaaacaatg
     3121 gatgactttt cgccgatata tcacgtcaac aaatcaattc gtcaaacgac attagttgat
     3181 ctaatcaaaa aagggtttca agaatacggc acattgattg aagaaaatct gccaaacaac
     3241 cttgtggaga aatatcgact gcttgaccga gcttctgctg taagaagtat gcattttcca
     3301 aaagaccacg aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta
     3361 tttcaaatgc gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag
     3421 atcctttatg atgtccaaaa attgaaacaa tttactcaaa agctaccttt tgaactgaca
     3481 aatgcacaaa aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg
     3541 cagcgacttt tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta
     3601 tacgctaccg ttacagcagg tttccaaggt gcgttgatgg tgcctacaga aatcttggca
     3661 caacaacata tggaaagctt gcaacagctg tttgatccca atgaagtaac catcgcattg
     3721 attacggggt caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg
     3781 gaaattaacg tcgtgatcgg cacacatgca ttgatccaag atggagtcga atttcagaat
     3841 ttaggtctag ttattacgga tgagcagcac cgttttggcg tgaaccagcg gaaagtactt
     3901 cgtgaaaaag gctggcgacc agatgtctta tttatgacag cgactccgat tcctagaaca
     3961 ttagctatta cagcatatgg tgagatggat gtatctatca ttgatgaact tccagctgga
     4021 cgtatcccga tcgaaacacg ctgggtaaga acacctcaat tggacagtgt actggattgg
     4081 acgaaaaaag aactggcaag aggacatcaa atgtatgtca tctgtccgct gattgaagaa
     4141 tcagaagcat tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg tgaactattt
     4201 gcaccaaaat atgaagttgg cttgttacat ggtaaaatga agaatcagga aaaagaagta
     4261 atcatggaag ccttcaaaga caaccagatg cagatattag tttcgactac tgtaattgaa
     4321 gtaggggtaa acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga tcgatttgga
     4381 ttggcacagc tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt
     4441 atcttagttg ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat catgacagag
     4501 accaccaatg gttttgtact aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta
     4561 tttggcttcc gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt gacggatgga
     4621 aatgttttgg aggtagccag agaagaggct gcagcccttt ggcaaataaa ggattggcag
     4681 atgcttcctg agtatcagcc tttagcgcaa agcctccaaa tagaaaatca ggataatcgt
     4741 tatttcgatt agcagactgt gctatacttt atactgatga cggaaaaagg agtggaaaca
     4801 atgaaaattg ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc
     4861 gtggctttag cgcagcaaga ttttccagaa gtagaattct tactttacgg aaaagaagca
     4921 gaaatcaaga aatacttaac aaatgaaaaa aacatcacga ttatccatac agacgaaaaa
     4981 atcaatagtg atgatgagcc agtaaaagca attcgacgaa aaaagactgc atcaatggtc
     5041 cttgctgcac aagcagtgaa aaacggtgaa gcagatgctg tattttcagc agggaataca
     5101 ggcgcgctac tagcagcagg gttgttcatc gtaggacgta tcaaaaatat cgaacgccct
     5161 ggcttgatgt ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga tatgctggat
     5221 cttggcgcta acgcagaaaa caaaccagaa catttgttgc aatacggtat tttaggttct
     5281 ttttatgcac gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga
     5341 acagaagcaa caaaaggtag tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa
     5401 acaagcttga attttattgg aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat
     5461 gtagtggtca cagatggatt tacaggcaac gctgttttga aatctattga aggaacagca
     5521 atgaatatga tgaatctatt aaaatctgct attctaaatg aagggatcaa aggaaaaatg
     5581 ggcgctatgc ttttaaaaga cggcttacgt tctttgaaag cagaaatgga ttattccaaa
     5641 catggcggtg ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca tggagcaaca
     5701 ggccccgatg ctgttcgcta tacgatccgc caaatccaca caatgcttga gacagatgta
     5761 gttggacaat tagtcgaaca atttgaaaaa agcgaagaat agtatttgga tatctgaagc
     5821 gatccgttcc attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa attagccatg
     5881 gtgtaggtaa ttgacaagtg tatgattctt ggaggtgaaa aagttgactc gcgaagaagt
     5941 atttaacaaa gtagcgaaaa ttatttcaaa tcattttgag atagataccg ataaagtgac
     6001 ggatgaattg aacatcaaag atgatttaaa agctgattca attagtatta tggaattcgt
     6061 tttagagctt gaagatgaat tcggtacgga aatttcagat gaagacgctg aacaaattga
     6121 aacagtcggt ggcgcagtag attatatcag cagccatctt aaataatttc aaataaaagc
     6181 aaagaaggat aaaaagctgc atgcagcttt ttatccttct ttttttaatc tttttgacac
     6241 gtaaaagtca tttttctgat aattctggcg ataaacagaa attatgttgc aaagtaaagc
     6301 ggttttctat ataattatta ataattcatt acacaaagat aagaataacc tttgttataa
     6361 aacgatttat tctgtgtaat gtcctttttt attaagaaaa acttccattt aggaagcaga
     6421 gaggggtatt cgattgtccg ataaccaatt attagaagtt cagaacctac atacaggatt
     6481 ccgactcaaa gatgactatt acgatgcagt agatgacgtt tcatttactt tagacaaaaa
     6541 cgagatttta gcaatcgttg gggaatctgg gtgcgggaaa agtaccttgg ctactacgat
     6601 tatgggatta cttgatccta ataacacaaa aattacaggt gagattttat ataacgatct
     6661 gaatttaatc gacctgaacg aaacgctcta taataaaatc cgcggaaatg atatcggaat
     6721 gattttccaa gatccgttgt ctgcattaaa tcctctcatg agaatcgaag accagatcaa
     6781 agaaggactt tcctatcaca caaagatgaa tgcagaacag cgtcaagctc gtgcactaga
     6841 attgctagaa caagtaggga ttcctaatcc tgcgagagtt ggacgccaat atccccatga
     6901 actatcaggc ggtatgagac aacgtgtcat catcgcaatc gcaatcgcct gcaaacctcc
     6961 aatcgtcatt gcagatgaac cgacgacagc cctagatgta accatccaag cacagattct
     7021 tgatttactg aaagaccttc aggaagaaac aaaaacaggg atcatcttga tcactcatga
     7081 cttaggagtc gtagcagaaa tggctgatcg agtggcagtg atgtatggcg gacagttcgt
     7141 tgaagtagca ggtgtaaaag aactgtttga tcatcctaag catccttata cacgctcttt
     7201 actgaactct ataccacaag aaggaaatca tgatgcagaa ctccatgtga tcgaaggtgt
     7261 cgttccttct ctgaaaaaca tgcctcgtaa aggttgtcgg tttgcaccac gtattccatg
     7321 gataccagca agcgatcatg aagaagagcc tatccttcat gaagtcgcac cgaaccactt
     7381 ggtacgttgc tcctgctata agcatttcca ctttagagac gaaaaaggag aggtgtagat
     7441 gacagaatta atcacaatca aagacctgaa agttcattac cctattcgca gtggattctt
     7501 caatcgagta acggatcatg tatatgctgt agatggtgtg gattttatta tcgaaaaagg
     7561 aaagacatat ggtttagtgg gagagtctgg ctctggaaaa tctactacgg gaaaagccgt
     7621 tgtgggatta gaaaaggtaa cagccggaca aatcatgtat gaaggaaaag atgtaacgaa
     7681 gcgaagcaac cgcaaaaaaa tgggttataa caaggatgtg cagatgatct tccaagattc
     7741 aatgtccagt ttgaatccta aaaagcgagt gttggatatc attgctgaac ccatccgaaa
     7801 ttttgaacgg ttaagcgatc aagaagaaaa gaaaaaggta aaaggactgc tagatatcgt
     7861 tgggatgccg gaagatgcgc tatataaata tcctcatgaa ttttcaggcg gacaacgtca
     7921 acgtctagga gtagcccgtg ctgttgctac caatccaaaa ctgatcgtag ccgatgaacc
     7981 tgtatctgct ctggatctat ctgttcaagc gcaagtattg aatttcatga aacgaatcca
     8041 gcaagaatac ggcctcagct atttattcat ttcacatgat ttaggtgtag taaaacacat
     8101 gtgcgacaat atcgcaatta tgtacaaagg tcgatttgta gaaatcggta caagagaaga
     8161 catttataat gacccgcgac atatctatac gaaacgtctg ctttcagcta ttccgcaaat
     8221 cgatgtagaa aatagggaaa gccacaaaga aaatcgacga agagtagaac aagagtatat
     8281 ccaaaaccaa aaagaatact atgatgcgac tggtcgagtc tacgatttgc gtacgatcac
     8341 aactacacac aaagtggcgt tgaaagatgg aggtgcaagc taatgtggaa aacgattctg
     8401 cgacgtgttt tattgatgat cccgcaagtt attattttaa gtgttctgat cttcttatta
     8461 gcccaagcga tgccggggga tccatttacc ggtttgatca atccaaatca ggatccggca
     8521 gtgatcgaac agatgcgaca agcagctggc ttgaatgacc catggtatgt ccagtacttc
     8581 cgttggatcg gaaatgcttt gcaaggcgat tttggtcaaa gtttcttata caaacgctct
     8641 gttgctgact tgatcggcga acggatcatc aacactttat atctatcgat cttgacagtt
     8701 gtccttactt atttgattgc tgtgcctctg gggatgcttg ctggccggta tcaaaattca
     8761 atcttggata agattgtcgt aatctacaac ttctttagtt ttgctgttcc tttatttatc
     8821 ttcggcttga tcatgctatt tgtttttggt tatcgtttag gctggttccc aacaagtgga
     8881 tcacaaacac taggtttgca aggtggattt atcactcaat ggatagaccg tttgaaatat
     8941 attctgttac catcaattac ccaagcattt ttggcgactg ccgtcacaat ccaatatctg
     9001 cgaagtgaag taatcgattc gaaatcgctc gattttgtca gaacagcccg ttccaaaggg
     9061 gtaccgacga ataaagtatt cagtcgacat attttccgaa atgcagcgtt gccgatggct
     9121 tctcaaatgg ggtatgaaat cacttcgttg attgctggtt ctgtagttat cgaaaagatt
     9181 tttggttatc cgggagtcgg aaaactattt attgattcca tcggccaacg ggactatacc
     9241 gtgatcacat ccttagtatt gattttagga atcgcgacgc tagtggggac attactttca
     9301 gatatcatca tgagtatcgt tgacccgcgt attcggattc aataaaacca atcagaaagg
     9361 agaatggatc gatgagtgaa aaaaacaata cagcagttca agaaagtatt cctcctatgg
     9421 ggatacgaat gattgctcga gaatttaaaa aagataaaat tgctattttt tcattaggtt
     9481 tgctagtggt tttattatta gtgatcttta tcggtgcgat gctgaccgat caagacaaag
     9541 tgatgtatgt tagtatcttc gacaaatacg ctgcaccagg aacagtttcg acacaaggac
     9601 aaaagttcat tctgggagca gatgaaggcg gacgtgatgt attaggacaa ttgatcatag
     9661 gtgctagaaa ctctgttacg atcggatttg cgatcacgat ccttacttcg atcatcgggg
     9721 ttggattagg gatcatctct ggctactacg gagggatcat tgacaacatc ttgatgcgga
     9781 tcgttgattt tatcatgatt ctaccgatca tgctgatcat tatcgtcttc gtatcgatta
     9841 tttccagata caatgtctgg tcctttatat tcatcatgag tgcattttat tgggtagcta
     9901 aagcccgatt attccgaagc aagacattat ctgaagcaag aagagattat gtcagtgcct
     9961 caaaaacaat gggaaccaat gattttaaaa tcatgttccg cgaaatcatg ccgaatctaa
    10021 gttcattaat cattactaat ttgacgatga actttgcggc aaacatcggt atcgagacaa
    10081 ctttgacttt cttaggtttc ggcttgccgg caaatgtacc tagtttaggt acattgatcg
    10141 gttatgcaag taacggagaa gtcttagtga atcggcaatg gatctggtta cccgcatcaa
    10201 tccttatttt agttttgatg ttgagtatca attatgttgg acaagcattc aagcgttctg
    10261 cagatgcgcg ccaacggtta ggctagtata gaagcgaagg gggaaatagg aatgaagaaa
    10321 acgatttttg gatttgcagc gctgatgtct gctgtcgttc ttgcagcatg cgggggcgga
    10381 ggtacagata cctctggaaa caactctgga ggcaacggca ataacaaaga ggaattaact
    10441 tttccaattg caacaaaaaa taatgatgat gcaatcgaag acggcgaatt agaagctgcg
    10501 gtcgcaacgg acagtcagtt taaaggattg ttccagtggg agttttacca agattcatat
    10561 gatgcagctt ttatggctcc ttctcatgaa ccgctatttg caaacgatga cgatttcaga
    10621 atcacagacg acggagctgc aacgcttgat ctagatgaag atacgaaaaa agcgacaatc
    10681 aaagtaaaag acaacgtcaa atggtcagat ggtgaagatg taacggcgga cgatgtgatt
    10741 ttcccttacg aaatcattgg gaataaagat tacactggta tccgttatga cgacactttc
    10801 cgtaacatcg taggtatgga agaatatcat gatggaagtg ccgacacaat ctccggaatc
    10861 aaaaaaatag acgacaaaac agttgaaatc gaatataaag aagtaaaccc aagcatgctt
    10921 caatcgggtg gtggtatctg gtcatatgca gcaccaaaac atacgttaga aggtgtagca
    10981 atcaaagata tggaatcaag cgatcaagta cgtaaaaatc cagtgacttt tggcccttat
    11041 tacatgagta atatcgttgc aggagaatca gtagaatact tgccaaacga gcactactgg
    11101 aatggtacgc ctaaattgaa aaagatcacg atgaaatctg taccgatggc aagtgcaact
    11161 gaagcattga actctaaatt atatgatatg attttccaaa tgccaacaga tacttacgat
    11221 acctataaag aaattgctgg ctatacaaac ttaggccgtg aacaaacttc ttatacttat
    11281 ctaggattca agttaggtaa atggaatgca gatacaaaga gtgtggaata caatccagat
    11341 tcaaaaatgg ctgataaatc attacgtcaa gcaatgggct atgcattaga taataatgca
    11401 gtaggtgaac gtttctatgc aggtttgaga agcaatgcga cttcattgat tcctccagta
    11461 ttccagtcat accatgatga tgagatgaaa ggattcacac aagatctaga caaagcgaaa
    11521 aaactattag atgatgctgg ctacaaagat gtggatgacg atggtttaag agaagacaaa
    11581 gatggaaata aactaacaat caactttgct tcgatgtccg gtggggaaac agcgcaacca
    11641 ttggcagatt actatatcca acaatggaaa gaaattggtt tggatgtcca atacacaaca
    11701 ggtcgtctga tcgaattcaa ttctttctat gataaattag aaaacgatga tcctgaaatt
    11761 gatgtttacc aagctgcttg gaatacagga actgatccaa atccaagtgg actatgggga
    11821 ccaaatgctg cgttcaacta cactcgttat gaatcagaag aaaatacaaa attgatcaac
    11881 gatatcagtt caagcaaagc atttgatgaa gagtaccaaa aagaagcata tcaaaaatgg
    11941 caacaatatg catttgaaga agcatttgcg attccaacgt tgttccgtaa tgaagtattg
    12001 ccagtcaacg accgtgtcaa agactgggat tggtcttatg atgtaccaaa cccatgggca
    12061 aatgtttctg ttacagcaga ttcacgttca taatcactaa aaatgaccga aaaagggcga
    12121 tgtccttttt cggttttttg atatttttct gtttatttct ctaatattca ggtcaaggcg
    12181 ctgatttcat gacttctcca ataaacggtg ttcaaagtcc agtttctcgg tattaagtca
    12241 aatttatgca aaaatatgga aagccatttt cacaaaattt tttcttcata ctcgaaacta
    12301 aacgaatttt tcacaacttc tcgtcaatca ctaggattat taaagcttta cttacctttt
    12361 gaccgaacca tttcaaattt gacaaaaaac cagtataata aggaagtatc gaaaaaatat
    12421 ccataaaagg aatgtggaaa atggacaacc agctgacaaa agaactaaaa gaaaactata
    12481 acatcgtttt tcacgatcta aatttgcttg aacaagcttt tacccattca tcctatgtga
    12541 atgagcatcg caatctgcaa ttatcggata atgagcgact tgaattttta ggagatgccg
    12601 ttttagaact gatggtttct cagtatttat tccgtttata tcctcatttg ccagaaggaa
    12661 aattgacaaa aacacgagct gcgatcgttc gcgaagacag cttgtcgaaa tttgccaaag
    12721 aatgtcactt tgatcaatat gttttgttgg gcaagggaga agagaattca ggaggacgta
    12781 cgcgaccagc attgctttgc gacctattcg aagctttttt aggcgcgtta tatcttgacc
    12841 agggattcga cacgacccat gcattcatcg aaaaagtgat ttttcctaaa gtcaaagcag
    12901 gtgctttttc acatgagatg gatcataaaa caaaactaca agaagtcttg caaaaatcag
    12961 gtgatgtatc aatcgaatac cgattgatca atgaagaagg tccagctcat gaacgagtat
    13021 tttggatcga agtttatgta gatgatcaat tgattggcac tggacaaggg aaatcgaaaa
    13081 aattagcaga acaagctgcg gcggaaaatg cattggcagc actgtaaaag taagtgaggg
    13141 aaagctttgt atttgaaacg aattgaaata gccggtttta aatcatttgc agacaagaca
    13201 gtcattgatt ttgaaaatag cgtcacagca gtggttggac caaatggcag cggaaaaagc
    13261 aatattacag aggctatccg ctgggtatta ggagaacaat ctgctaaaag tctgcgaggc
    13321 ggaaaaatgc cggatatcat ctttgccggt tccgataccc gtaagcagct gaatattgca
    13381 gaagtcacag tgattttaga caatagtgat cattatctgc cattggagta caatgagatc
    13441 agtgtaaccc gccgttatcg acggactggg gaaagcgagt ttttcatcaa taaacaaagc
    13501 tgcagattaa aagatatcca agaattgttc ttagattctg gattaggaaa agaatcgttc
    13561 tcaatcattt ctcaaggaaa agtagaagcg attttcagta gtaaaccgga agaccgtcgt
    13621 gggatctttg aagaagcagc tggtgtcttg aaatacaagc agcggaaaaa gaaagcagaa
    13681 cagaagcttt ttgaaacaga agacaacctc agccgcgtcc aagacatcat ccacgaattg
    13741 gaagaacagt taactccttt ggccgcccag agtgaggcag caaaggagtt tttacggcta
    13801 aaagaaacac tgactcaaac cgatgtctct ttgatggttg ctgaaatcaa gacagcaaaa
    13861 aaagattggg acaacaaaca agcacagctt gcgaaattca atctagaatt aggcaaacta
    13921 tctgagtcga tccaagaaca agaatccatt ttggcgaagc agcgtaaaga aaatgcgcaa
    13981 gccgatcgtc tgatcgaaaa aaatcagcaa gtattattag atctttcgga aaaactgaag
    14041 cagacagaag gacaaaaaga tgttttgcag gagcggacaa agcatacaca aaaaagctcg
    14101 caagaatatc aaacttcatt agcagaagca caaaaaaagg ttaaacattt tgaaaagctt
    14161 caagaaagct tgatgaaagc agcagcagaa aaagaaacag agatccaaaa agctgaagca
    14221 aatctcataa aaacacagca agaattagaa aaataccaaa aatcaacgaa agaattattg
    14281 gcagaattaa gagatcaata tgttgatttg atgcaagaac aagcagctgt aggaaacgaa
    14341 ctgaagtatc tagaacgtca atatatacaa gaaacagcaa aaagcaagca gacgttagcc
    14401 aagcaatcgg aagtagaagc atcagtagac cgattgatct tacaaaaaga agaattgaca
    14461 caaaagcagg cgcagctaaa atcatcttta actgaaacaa aagagaagtt agaaatgatc
    14521 caacaaaacg ggaaaaagtt ccaagaagca ttagcaaaag aacagcctaa aatgtatcaa
    14581 ttaatgaatc aagtccagca gctacgtgct cgtaagaaaa gccttcaaga aatccaagaa
    14641 aactattttg gtttttacca aggggttcga ttggtcttgc agcataaaca gcagctttcg
    14701 ggtattgtcg gagcagtggc tgaactgatc gatgttcctg cagatttcac gttagctatc
    14761 gaaacagctt taggtggggc agcccagcat gtcatcgttg aaaatgaaaa agatgctcgg
    14821 caagccatca cgtatttgaa acagcaaaga ggaggaagag caacattcct tccactgacg
    14881 acaatcaaac caagacaatt accggctcat atattgacac aagcagcagc tgtagaaggg
    14941 tttataggga ttgccagtga acaagtcagt tatcccgatc aaatccaaac tgtggttcaa
    15001 aacctgctag gaacgatcct tttagccaaa gacttaacta gtgccaatgc aatcgcgcaa
    15061 acgatccgct atcaatatcg agtggtttcc ttagaaggtg atgtgatgaa tgccggagga
    15121 tccatgacag gtggagcgaa taaacgagga aaccaaggca gtttgtttgt acaaaatcaa
    15181 gaattgaaac agttgactag tgaatttgaa gaagcagata aacaactcca agcacaagaa
    15241 aaaaaagttc aggagctgca acaagaaaca gctcgtttag ccgaagaaca agaagttttg
    15301 cgtacacgag gcgaacaatt acgttttgaa gaacaagaag cgacaaatca gctacaaaac
    15361 atcataaatg aacttgaacg ttttgaaaaa gaaaagcaaa tctcaacttt tgagacacgt
    15421 gaactgcagc agtttatcga agattacgaa aagcaaacga acgaattgaa agacaaacaa
    15481 acagatcttg aatcgcaacg acaaaaaatc gacgaagaaa tcaaatcgtt aagtcaagaa
    15541 agcgaccaga tggaagccag aagagcacaa gtacaaagtc aaaaagcaca agaacaagca
    15601 gatcttgctg tcttgaaaga gcagtttaat catctgcaga tccagctccg cggtgcccga
    15661 gtccaaaaag cagaggcaac cgaacgtcaa gaagcgatag aaaagcagtt ggcgacattg
    15721 acagcagact tttctgatca tgaagtcaca gaagaaagtt tggaaaaaca aattaacgaa
    15781 ttatctgctc agcgagaaac attaaaagcc gagttggcaa aagccaaaga gcagcgagat
    15841 agaaggcaaa aggagatcga taagcttgaa gcagtcttgg cagagcgtaa tcgtgagcaa
    15901 aaagcacgac tgtcggaaca atccaaatta gaagtccaaa aagaccgagc tgaaatgatt
    15961 ttagataatc atttgaccta tctgcagtca gaatatcagc tgacatttga gaaagcttcc
    16021 caagactatc aagaaacaac cgatatcgaa gacagtagaa caaaggtttc atccttaaaa
    16081 gaacagattg aaaaactggg accagtaaac ttaaattcaa tcgaacaata cgaacaagtc
    16141 agtgaaagac atacattctt agcaacacag cgagatgatc tgttagcagc gaaaaaccaa
    16201 ttgtttgaaa cgatggatga gatggatgat gaagtgcgga caagattcaa agaagtattt
    16261 gaagctatcc gtcaagaatt caaaattgtc tttccaaata tgtttggcgg cggtaaagct
    16321 gaattagtac tgacggaacc aactgatttg ctgaaaacag gtattgagat tgaagtacaa
    16381 cctcctggca aaaaactgca aagtctaagt ttgctttctg gaggagaacg agcacttaca
    16441 gccatcgcgt tgctcttttc cattatccgt gtctgtcctg ttccgttttg tatcttagat
    16501 gaggtagaag cagcgttaga tgaagccaac gtgaaacgtt ttggtcgtta cttgagcgat
    16561 ttccaagatg acacacaatt tatcgtagtc acacaccgca aaggaacgat ggtcgcagca
    16621 gatgtactgt acggagtgac gatgcaggaa tccggtgtat caaaaatcgt ttctgtacgg
    16681 atggaagata tcaatgaaga ggcgaaaatc aacgaaacga aagtgtagga acgaaaatga
    16741 tcaaattagt agcaatcgat ttggacggta cattattaaa tgaacaaaag ctgatttcag
    16801 atgagaataa acaagcatta gcacaagcaa aacagcaagg ggtcaagatc gttctatgta
    16861 caggacgccc cttagctgcg atggcgcatt atctgcaaga attaggactg gtagatgaag
    16921 gtgactacag catcactttc aacggcggtt tggtacaaaa aaatgatact ggtgaaatca
    16981 tggaaaagaa agtgatggaa gtatcagata tccaacgctt atacaaactg gctcagcaat
    17041 tggatctgcc actagatgtt ttgtctgaca atgtggtttt acagctgcct agtgccccaa
    17101 ggaagcaatc cttgtacaat gtgttgaata agctgctgca attccaacca gcggatataa
    17161 gcgatattac agatgaattt gtgcttaata aggcagtaat cgcttatgac caagaagaat
    17221 tagatcctaa aatcaaagaa ataccagcag aattccatga aatgtatgag atcatcaaaa
    17281 caagaagtat gcttttggaa tttatgccaa aaggtgttac aaaagcttat ggtatttctt
    17341 tattggcaaa agatcttggt ttgaaacaag aagaaatcat ggcgatcggt gatgaggaaa
    17401 atgatttacc gatgatccaa tatgctggac taggcgtagc gatggagaat gctgtgcctt
    17461 tcgtcaaaga agcagcagat ttcgtgacag ccagcaatga agaaaatggc gtagccaaag
    17521 tgatccaaaa atttgtatta gagtaggggg agtcaaatgg gattttttga taaaatcaag
    17581 cgtgctttta ctggcgaaga gaaaacagaa gaagttgaag tacaggagaa gtacgacaag
    17641 ggtttagaaa agacgcgtaa aacatttggt gaacggatga atgagttgtt tgccaatttt
    17701 cgaacagtcg atgaagactt ttttgaagaa ttagaagaaa cacttatcgg tgcagatgta
    17761 ggttttgaaa cagcgatacg aattaccgaa gcattacgtc aagaagtgaa actcagaaat
    17821 gcgaaaaaat cggcagaagt ccaaaatgcg atcatcgaga aacttgtgga tctctatgaa
    17881 gcggaaggaa tcaatgaagt aaatgaacta aatatccaag aagacggact aacagttatt
    17941 ttgtttgtcg gtgttaatgg tgtagggaaa acaacaagta ttggtaaact tgcccatcag
    18001 ttcaaaaacg aaggtaaaaa agtattgatg gcagcagcag ataccttccg cgctggagca
    18061 atcgaccagt tagttgtctg gggcgaacgc gctggtgttg aagttgttcg tggcaatgca
    18121 ggaggcgatc cagcagctgt cgtattcgat gctttggaaa gagcaaagac agagcaggca
    18181 gatgttttac tagttgatac tgcaggccga ctgcaaaata aagtgaactt gatgaacgaa
    18241 ttagaaaaaa taaaacgagt aatccagcgg gaattaccag aagcacccca agaaacactt
    18301 cttgtattag atgcaaccac tggtcaaaac gcgatggttc aagcaaaaca attcaaagaa
    18361 acaacagatg tgaccggtct tgttttgaca aaattagatg gtacagcaaa gggcggtatc
    18421 gtacttgcta tccgtaatga attgcattta ccagtgaaat tggtcggtct tggtgaagga
    18481 atcgatgact tggagccgtt tgatccaaat gattttgtcg ttggattgtt caaaggatta
    18541 ctaaaagaag aataacccat gtctaaaaag caggagctgt gacaaaagtt gcagctcctt
    18601 gtttgaatga ttgtttttaa catagctgtg tttttgatgc cttcattttt atacttattt
    18661 gtaagcggtt ctcaaacaat ttcgttcttt ttcttagact ttttgattat aattagattc
    18721 atatgaattg gaggtcactt aatgatgaat tttaagcaat tattattgca tgtcaatgcg
    18781 cgccctttta tcgaccaagc tcgatttggt atagaacggg aaggacagcg ggttgatctt
    18841 gcaggaaatc tagcaaaaac cgatcatcca gcaatctttg gcgatcgatc ctatcacccc
    18901 tatatccaaa cagattttag cgaaacacaa acagagatga tcacccctgt taccgattct
    18961 attcccgaat tatttcagta tctcgctgct gtttatgatg tgactgctcg ttctataccg
    19021 aaagaagaga tgatctggcc attaagtatg ccacctgcct taccggaaaa agacgaagag
    19081 atcattattg caaaattaaa aaatttcgaa gatgtcttgt atcgacgtta tttagcaaaa
    19141 gaatacggga agcgaaaaca gatggtaagc ggtattcatt tcaattttga atttggcgat
    19201 gagttgctaa gaacattgtt cagccatcag gaagaatttc aagatttttc cgaattcaaa
    19261 acagaacttt atttgaaaac agcgagaaac tttatgcgct atcgctggat gatcacttat
    19321 ttattcggtg cttcaccaat gagtgagaag aactactttt tagatgaatc acatccgcaa
    19381 gaacctgttc gcagtattcg caacagtgcg ctgggctata cgaatcatcc aaatgtgaaa
    19441 gtttcctatg cttctatgaa acagtattta gcagatatcg agcgaatgat cgaagaaggg
    19501 aaactttcgg aggagaaaga attttataca ccgcttcgtt tccgaggcgg aaagaaagtc
    19561 gcagatttag caacaacagg tgttcgttat attgaattgc ggaatatcga tttgaatcct
    19621 tatgcaagat tgggtatcaa tccagagcaa gttcggttct tacaattatt cctgatgtac
    19681 atgttatgga cagaagaaaa agaagactgt gaccaatggg tagcagaagg tacgactcga
    19741 aataacaaag tagcactaga acaaccatca gatcaaacag aatttcatca agaaggtaga
    19801 gaaattcttg aaggaatgaa gcaaatgctg gttgaattgg attggttgga ttctctttat
    19861 ttagtagagg aagcattgac tcaaatggat catcctgaac aaacgttagc agcgaaactc
    19921 tatcaagaag cacagctatc gagtcagcaa gaagttgctg tggcattggg acatcaatat
    19981 tataaagaaa gccatgaacg tccctatcag ttggccggtt ttcgcgaaat ggaactttct
    20041 actcagatat ttatgttcga tgccatccaa aaaggtgtcc aagtcaaagt attagatgag
    20101 tcggatcaat ttttgcgtct gcaattccaa gaccatgtag aatatgtgaa aaatgcaaac
    20161 atgacaagta aggacagcta tatcgtccca cttatcatgg aaaacaaaac agtaacgaaa
    20221 aaagtcttaa aagaagctgg gttccgggta ccaggaggcg cagaattttc atccatggaa
    20281 gaagcggtaa aagcttatcc gagatttgcc gagcaggcat ttgtgatcaa acctaaatca
    20341 accaattacg ggttaggtat cacgattttc aaggaaggag ctagtctgga agattatcaa
    20401 gcaggtttag ccattgcttt tcgtgaagac agttcggttt tagtagaaga atttatgccg
    20461 ggaacggaat atcgtttctt tgtgatcgac ggagaagtcc aagccatcat gttgcgagtc
    20521 cctgctaatg ttattggtga cagtatccga acagtgaaag aacttgttga agaaaaaaat
    20581 agtgaccctt tgcgtggaac gaatcatcgt gcacctttag aattgatcca attaggtgaa
    20641 ttagaacaat tgatgttgaa agaacaaggg ttgacgatcg aatcagttcc ccaagccaat
    20701 caaattgttt atctaaggga gaactcaaat atcagtacag gtggtgattc gatcgatatg
    20761 actgatgaat tttctgaagc ttacaaaaaa atcgccgttt ctgcggtaga agcactagga
    20821 gctaagatta gtggtatcga tttgattata ccagataaag aaatcgatcc tacaactgat
    20881 aaaaaagcat atgggatcat cgaagcaaat ttcaatccag ccatgcatat gcatgtttat
    20941 ccttttgcag gaaaaggcag aagattgacg atgaatgtat tgaaattgtt atatcctgaa
    21001 gtattttaaa agaaaaagct tttgacagat tgtcctattt gatgtcaatc tgatcaaaag
    21061 ctttttttgc atgtaaaaat ttatgtgttt tttcgtttta aaataaacga tcccagaagc
    21121 tttttttctt ttttggtttt tctttttcag ctggcgaaga gttttcttct gggtattttt
    21181 tttcgatatc aatcacaggt tcatttactg cttcttttgc tgttaagagc aatccgaatt
    21241 cctcaggatt tgcagtgacg taatcgttga ctacagtgaa acggctattg tttttcgttg
    21301 ctagctggat atattggttt tggacagaaa ggggtagagc cccattcaag agtatggaag
    21361 catctggatg ttttttgagt tcttttaaaa agttttcttt gttttttggt tggatcattt
    21421 cagcaacagt catactaaga tagcagcgtt ccctgaatgt ccctaaatat ttatgctgtt
    21481 cttcagggtt cactaaaggt gtaccataaa gtcccttatc caaatggctt tttacatcat
    21541 cttgtgccat aaaaaatacc ctccttcttc tctttgtctt tcttacgatt atagcatatt
    21601 cccaataata cctaaaacat atgaaaaagc ggttgctttt gttcaacgat tcacttaaaa
    21661 taagaatgaa aatcattctc aattagaaag gtggattgga tgcgctacgg acgactagta
    21721 gcattattag ttattttaag tagtatttca ttgttcatcg gagtatcgaa tgtacaattg
    21781 tcagaactgc tccagttaga tgggcaaagc ttacaaatct tgctagtgac tcgtttgcca
    21841 cgaacgattt gtttgatcct tgtgggagcg accagtagta tttgtgggtt gatcatgcaa
    21901 catttgaccc aaaataaatt tgtttcacca acgactgcgg gcacgatgga tagtgcgcgt
    21961 ttaggtattt tagtggctat gattttccta cctggtgcat ccttgattgt ccgatccttg
    22021 acagcatttt gctttgcttt tgcaggaaca ttgctttttt tatcccttac gcgattgttc
    22081 ctccagaaaa atcaattgat gcttccgttg attggagtca tgttagggaa tatcattggt
    22141 tcagttgcta ctttttttgc ttatcaattc cagttggtac aaaatatgac ttcatggctt
    22201 caagggaatt ttgcgactgt gatacgagga aattatgagt tattgtatgc tgttgttcca
    22261 ttgttgctct tggtttggct atttgcttac cgatttacga tcattggtct gggcgaatcg
    22321 tttgcagtaa atctgggaat agattaccag aagatgcagt ttttaggcat tggcttagta
    22381 tcattggcta gtgcagtcat gttgatcatg gtaggaagca ttccttttat cggagtgatc
    22441 attccgaatc ttgtctctag aatatatgga gatcaagtgc acaaaacaat tggcgtaact
    22501 gctttagcag gtagtttgtt cttagtattt tgcgatcttg tcgctcgcgt gttgattttt
    22561 ccttatgaaa taccagtcag tgtgatcgtt ggaatcatcg gtggggctat cttcctttat
    22621 cttctgataa ggggacgacg atatgcttaa aaaaaagcca tctattttta ttggtatgct
    22681 tgttcttgtt ttatttgtta caggaatgta cttgacgatc catacatatg gaaactggca
    22741 ttttgcatgt gcattgagga gtaagaaaat ttttgcattt atccttgtct cgctagctgt
    22801 cagcagtgcc acaatcagct ttcagacaat tacgcataac cagtttttga ctccttcagt
    22861 attgggactt gagtcgttat atgtccttgt ccaaacggtc cttttcttct ttatcggagg
    22921 agtgacgatg cttcagcaga caggtacagt ttttttctta ttgaacgttg ggctgatggt
    22981 cgggctgagt ctattgttat ttttctttct tttgaaaaaa atgagagcga atcttttttt
    23041 attactgatg tcagggatga ttttaggtac gctttttggt agtgtcagta cctttttaca
    23101 agtcatcatg gatccgaatg aatatgattt gcttcaaggc agattatttg caagctttgg
    23161 caatatccag agtcagtatc ttttgcctgc ttctttgatc attatcggct gctggtacct
    23221 gttatttcga tatcgacatg tgctagacgt tttccatctc ggaaatgaac aagcagtaaa
    23281 cctcgggata gacacagatc gttttgaatt tttgatttta tgttgtgtca gtcttttgac
    23341 agggacatca acggctcttg tgggtccagt tactttttta gggtttattg tcgcaaatat
    23401 cagttatcgt gtcttttcta cttatcgtca tggtcagcta tttatcggaa gctttctggt
    23461 agctatgctg tttctgacag gaggacagct cgcagtagag cagattttcg gctggaatac
    23521 aacagtgagt gtcgtcgttg aatttgtcgg aggaatttat tttgtcacaa aattgattcg
    23581 cgaaaggaag agtatcctat gattgattta caaaaggtca ataaactgta ccatggaaaa
    23641 tcagtggtta aagatgtaca gttaacgatt cctgaaaaga aattgactgc ttttatcggt
    23701 ccaaatggtg ctggaaagtc aacggtgttg tcgatgatca gtcgtttgat accaaaagat
    23761 acaggtgaaa tctatttaga ccataacgaa gtaaaggctt ggaaatccaa tgaattagcc
    23821 aaaaaattat cgatcctacg acaatcgaat gaaataaatg tccaaatcac cgtgaaagaa
    23881 ttagtagaat ttggacgatt tccttatacg aaaggaaaat tgacgacaaa agacgaggaa
    23941 atcgttcagg aatcactgga acaccttggg ttagaagaaa tagcggatca atcgattgac
    24001 actctttcag gcggtcaact ccaacgtgcg tatattgcaa tggtgctagc gcaagacacg
    24061 gattatattt tactggatga acctttgaac aatctagata tgaattttgc ggttcaaatc
    24121 atgcagattt taaaaaattt agtacaagaa atcgggaaaa caattgttat tgttcttcat
    24181 gatatcaact ttgctgcaag ttatgcagac gaaattgttg caatgaaaga tggacagtta
    24241 tttacgcaag gaacgaccca agaaatgata aaaaaagatg tattggatga attatacgaa
    24301 atggacatac ggatttgtga gattgaaggc cgcagattct gtctttattt ctaaaactaa
    24361 cattaggaga gaagagatga aaaaattagt agtaggtatc ttgattctag catcatttgg
    24421 tttagcagca tgtggaaata gcaacaacac cagtcaggca gacacaaaaa aaagcagcac
    24481 acaaactgaa actacattga cgataacaga tagtaatgga gaccaaatcg aggtgccaaa
    24541 taatccagaa aaagtcgtcg tattcgacaa tggttcttta gatacgatgg atgctcttgg
    24601 tgttggagat aaggtagttg gagcagctac aagcagcctt cctgagtatc tttcatccta
    24661 taaaaaagta gaatcagcag gtggaataaa agaaccagac ttagaaaaaa ttaatcagct
    24721 tcagccagat ctaatcatca tttctggccg acaaagagat tttcaaaaag atctaagtca
    24781 aattgctccg accattttcc tttctttaga tgcgaaaaat ccatgggaat ctttccagca
    24841 aaatgtcact gctttagggg agatctttgg taagcaagaa gaagctaaaa ctcaattaga
    24901 ggaattatct agtgcaatcg atcagacgaa aaagaaagca gaagctactg acaaaaaagc
    24961 actagtcaca cttgtgaatg aagggcaatt atcagcctac ggatctggtt ctagattcgg
    25021 tttcatccat gatttatttg gctttgaaca agcagatgat caaatcgaag catccactca
    25081 cggtcaaagt gtttcttatg aatatgtgct ggaaaaaaat ccggatatct tatttgtagt
    25141 agacagaaca aaagctattg gtggagacga cagcaaagat gatatttctg ccaatgaact
    25201 ggtagcacaa acgaatgctg gtaaaaatca gcagatcatc tcccttgaac cagatgtctg
    25261 gtatttaagc gggggcggtt tagaatcaat gaaactgatg atcgaagatg tgaatcaagc
    25321 atttaaatag cataaataac agaggattaa actctcatac tatgtaacaa taatgtttct
    25381 tacattctta aatagcaaga gcctatcctc aaagaagtta tcaaaaacca tccgtaaaat
    25441 cgtattgatg attttacgga tggtttttat ttatttgaga aatctttttt caacgtgaat
    25501 tcattatcta catcatacgg gttgattcta gaatggggag tggcaaaaaa tagtgtgatc
    25561 cattttttca gcatcaagat cgcctccttt gatttgaatc aaaggtacaa agcgaaactt
    25621 agataaaact taaatttaga tatttttatt ttgtaccgat ctcttcttta aacgatgttc
    25681 catctgctgc ttctcggcaa taagtccact agactagaaa atatgagaag ctattttctg
    25741 tccagcgttc ttattactca aagcaaatcg cagttgtcac aatctcttct aaacgatgtt
    25801 ccatctgctg cttctcggca ataagcccac tagactagaa aatatgagaa gctattttct
    25861 gtccagcgtt cttattactc aaagcaaatc gcagttgtca caatctcttc taaacaatgt
    25921 tcaatcagct gaccttcggt attagctcaa agcactgatt tcacaacctc tatttaggca
    25981 tgtgtctcag caagcggttg atagttgatg atctgcattt ttcgataaaa atcggcggga
    26041 agcttactcc accgttttaa taattgatag aaataactct cttctgcttt gattttatac
    26101 tcgcttaata tcaatccaaa actattcgtt ttttttgaag ctaatttttt ataaatatcc
    26161 aatgctttgg ttttatcttc attgacaaag agtgttttta cttcaatata agaaccctcg
    26221 atattttgtg tttcttcgat aaaatagatg ttttttatat attccttcat agtgattcct
    26281 ccaaagaagt cttttatttg ataagtttac tctaccgaag agttgttaat aatccgtgaa
    26341 caaaaagtaa ttctttttcc aatagttgtt aaaggttttt tattttttat agtatttttt
    26401 gttatttaag aaaggaataa ggttaaacaa ataacaggtt gctttttggg tctttttccg
    26461 ttcaagaaac tgaacgaaga aaagggaaag ttgtctcttt tgtgataaga gaaatcataa
    26521 taaacgaatg acttactttc caataaattg agaacagtca gaaccagaca aattagttgc
    26581 atctgatgaa taaagtgatt attctatgac atgaggtgat acagatgaaa ttagaagtga
    26641 caaaagaagc acaagaagta ttgaagaata aattagaagc agacgatcaa cttttattag
    26701 atatcgaaaa cggtgacggt ccatttgcag aaagccaagt cagttgccaa ttggatacgg
    26761 catttcgatt gatcattgtc aaaaaagata cacaaacaga tctaagtctt tattctgtag
    26821 tggctgatac accagttgga ccaatcaaaa tcaaagacag tgcaatactt tacatggatg
    26881 atccatccac acttgcttta gaaccaactt ataatagttt acaattaaaa ggaccaagcg
    26941 gcttgagaaa aggcaacttg caagttgttc gaaaagacta gagggataaa atgggaaaat
    27001 tggcgatcat cagcgaccta catgtggaca tcaataaatt cggcgaatca gagctgatgc
    27061 agctttggca agttttgcaa aagcacaaga tcactcggct tcatctagcc ggagatacgg
    27121 ctaataaagt agaccgttgc ctagctgttg tcgaattttt cgtcaataaa ggattatcta
    27181 cgacatttca tttcggcaat cacgagttgg cagatgtgcc caatgaacag atgatggacc
    27241 attttccaga cccacatttt ttaaatgaac ggtatattgc tttaaatgag cagaccgttc
    27301 ttttagggat gaatggctgg tacgactatc agttttccga attgaaagat tacgaggcaa
    27361 tcgtacgaat gaagcgtttg tattggtatg atcggttcat ccagcgtggg aaaacagatc
    27421 tcgaggtcaa tgcagaaatg cttgcagctg caaaaaattt gctagaccag ctggaagcaa
    27481 aaaaacttga ggtgatttta tccactcatt tcgttcctaa acgggcattt atcatgtatc
    27541 aatctgctcc ttatgagcgt tggaacaaat taaatgcatt tttaggatca gaatcattcg
    27601 gagaattact tgaccgttat tcaaatatca aacaagtagt ttttggccat acgcatcgtc
    27661 gctttgaaga taaatccatc ggtcacacga tttacagctg cagacctttt ggttattatt
    27721 atgaatggca gctgacccga gattttgtct tgaaagaaca gttgctagac agctatgatc
    27781 ctatgaaatt acgtgtcctc ttgcgtgccc atcacccagc ttttcaggcc tatcaaacac
    27841 aacatttgca agaggaattt gaacaagcga tgacaatcgt tccgtattga gacagcatta
    27901 gtaatgtgta aaagaaaata ccttgtaaaa cataaggata agtaagagca gtagatccat
    27961 ttatcgtgga tatgctgctt tttttgcgga aaataatcag cgaatgttta tttgatcgga
    28021 tgatttgtaa ctcgcagagt actagatgca agcgtgtatt ctttttttga acgataatca
    28081 caaaaaaaaa tataatatac attttttatt taattataat taatattgat tgaagtctag
    28141 gcagtcaagt cacattttga tagataggag ggggacttac gaattatttt aaaggctatg
    28201 acagctgtat ttctagattg tgtttgcatc aagttgcaat aaaaactaga aatcaaatcg
    28261 ggctgataat catttataac taatcgatac ataggaccaa atataggtct atgttctttt
    28321 atttcataag tatcgtaaaa cagaattttg ctatggatta atccaatcga ctgatatgtt
    28381 tagtaaataa agaaagagga atgtcaatga tgaaaaaaga aatcgtatta acagcagcaa
    28441 tgatgttatt cagcatggta gcaagtacga cttttgtttc tgcaactgaa gtatacccaa
    28501 aggaatataa cacagaagga acgattacat ttgaagcagg agatgagggt gtaactcctc
    28561 ctgttgatcc agagaatcct gatcccaata aacctgtcga tccatcagat cctccaagcc
    28621 ctggaacagg aggggcatta tcgattgatt atggatcaaa attcaaattt gggactcaaa
    28681 aaatttctac tgcggataaa acgtattatg cagctgctga tgtgatgaac gatgggtcta
    28741 gaaaaccgac atatgtccaa gtgacagata ggagaagtac cttatctggg tggaaattaa
    28801 gtgtctctca accagaacag tttaaaactg ccagtgggga tgaactagtt ggagcacagc
    28861 tgaaatttac gaaagggcag gctgtttctt tggttgatcc aacatatacg ccgcaaacgg
    28921 tgaattctga attaacgttg acaccgggag gaaacaatac gttagcaatt aatgctaaat
    28981 caggaacagg tgtggggact tgggtctatc gatttggtgc aaatgaaaat gaaaatcaag
    29041 atgcggtaca gctttcggta cccggtaaat ctgtcaagtt agcgcaacaa tattccacta
    29101 aattagtttg gacattagaa gatacaccta acaactagtt tactgaaaag aactgttaaa
    29161 aataaatacg tagtcatatt gtcttgcgag gtaatcgagc aaaaattgtc aaaggctagg
    29221 ctaaatttgt ttggatacga atagttcatc taggaattta acgatcagga ggaaaagata
    29281 tattgaaaaa gaggaggatc gtgctcattt taagcagtct cttagtgact aatatctttc
    29341 cgatcatagg gtactctttg gaattgaatc agttggagca tggaaataga agagacgaag
    29401 aaagcaaact cacttcgaca ttcgacagtt taatagaatc caaccagaca caagaaagca
    29461 gtgaggattt aagtatttcg agtagtggag agaccactga caacgtagaa gctgagggtt
    29521 cggacaaaca aggactagaa gatgcaccaa tttctgcggt tgagctgcct gacgatccca
    29581 atattatttc tatcgataaa atttttcaaa cacctatcgg tactagcacg tccatattgg
    29641 aaaatgggaa gttactgcag ttgaacccag cagaaaggtc tcaaagagga gctatttggt
    29701 caaataagaa gattagttta ctctcggatt ttacatttaa atcttattta tacttaggaa
    29761 atcaacgaga tagagcggga gatggcatga cctttacatt gactggcgat cctagaatgg
    29821 ctagtaatcc cagtcaagtt attggatcgc caggtatggg aattggctca tattctactc
    29881 gttctggcca accttacatt gcgaatgcgc tctcgattga gtttgatacg tataaaaata
    29941 gtggttcttc tgatcgaatg gatcgagaga taactgctga taatagaggg catggacatg
    30001 tcgcttttgt cacgcctaaa ccaaataata ataattattc tggagagcat actggcgtga
    30061 cggtagctcc tacatttctt tctaatggca catggcgctt gttaataatt aaatgggacg
    30121 cggagcaaag aagtcttact tatgatttgg aaggtgtcgg tcactcttct cggattatac
    30181 ctgatctcat taggcagttt ggttctacag aagtaaattg ggggtttact tcttcaacag
    30241 gtactttttt tcaagaaaat gctgtcgctg tcacgcgact tccatcgaat gtcacatcaa
    30301 cagctgatgt aaaagtcaac gatagtgatt atggggccat cgctgaagct ggaggaaacg
    30361 ataagataac tatcagaaat acattgaagg tcgaggggga ttttcttgaa tataagaaaa
    30421 tgcagactag tatcgacctc ccacaagaat taactattcc cgagcatatc ttgatcaatg
    30481 gaaatcaaat tgatgccaaa gatatcttga taaccggaaa tcgagtcacg ctaaatctga
    30541 cagattattt tattgctgga gatacgttaa taatcgaatt ggagacaact ttgcaggaag
    30601 taattcctga aaaagtactc actatgcatt ttgaatacct tgaaaacggg aatttgattg
    30661 agcaatcgaa tgaaataaac ctaaagatcg caaagctgaa ggagatagat atccatgttt
    30721 attataaaga taaagacagt cagattgata ttgctgaaat gaaaacaatc actgggaaaa
    30781 taggagagaa gtataaagaa actcctattg atattgaagg gtatgttttt gactcggatt
    30841 cagcgaatac agaaggcatc ttctccgaaa atacagagga tatctatttt tattacagaa
    30901 aaggcaaact ttatctctca actgctcctc aatatgtcaa ttttggaaaa cataaaatat
    30961 ccagtagacc attaagtgaa ttcggacagt tttcaggtgg gttgaaaatt atagatgaac
    31021 gggcttttgg aagttggaaa ttacaattga aacaagagaa agttcttaca aatggagatt
    31081 ttgagcttcc agaagctctt tcctttgtta cctctgaaaa tacaacggtt atagggacat
    31141 ccgctgttac gattttcgaa agtgatcaaa agggtgaaag cgatttgtcc tctcttttag
    31201 attctacagc tcatcgtgga atcagaatag atattcctgt agaaaaccaa agaatcggaa
    31261 cctttgaggg gaaactatct tggatacttg cagatgtccc aggaaattaa caggaagaga
    31321 agaatataac aaaattattg agtttcaaga agggcacagg taccataggc tcttgtgtcc
    31381 cacagagaca agattttgtt atagtcttct cttacatatg attaaaggag cgattttctt
    31441 gagaccaaaa caacaattat ttataacact cctagttatt ttaggggcac tttttaacaa
    31501 tatatcagtg tcagctagtg catttaattt ttcagtgacg ccgataactt cagagaatca
    31561 aatcgacaag agaaaaactt acttcgatct tcaacttgat ccggatcagg aagtagaagt
    31621 aaaagcggaa ttgagaaatg atacagaaaa agaagtgaaa atcgatattt ctgtgaatag
    31681 cgcaaccacc aattcaaatg tgatggttga atacggaaaa aacgagattg aaaaagacat
    31741 gagcctgatc tttgatctgg tagattatgt gagctatcca cagatagtga cgctaaaacc
    31801 taaaagtgtc caaaccgttg cttttcatgt caggatgccg aatgagcgat ttgatggtgt
    31861 attggctggt gggatcacat tccaagaaat ccagacggaa aaggaccaaa cagaaactaa
    31921 ggatcaaagc ttatcgattg agaatgaata cacctatatt attgctttgt tgatgcaaca
    31981 gacactgaac gaagttgctc cgaaccttac tcttcatgaa gttaaaccag atcaaatcaa
    32041 tgcaagaaat gtcatcttag caaacgttca aaatgatcaa aaaacctata tcaatcaagt
    32101 agttattgaa acaaaaatta caaaaaaagg acattctgaa gttttatatc aagaggaaaa
    32161 agaaggattg cagattgcgc cgaatacaaa tttttctttt ccaactgctt taaatggtca
    32221 accgctaacc ccaggtgaat atcaccttac aatgactgtt ttaggaaatg aaaatgaaaa
    32281 tggaaaattt tcgagaaaaa aaggaaatac tacgatcaac tataccaatc aatgggtatt
    32341 tgaaaaagat tttacaatag acggaaaagt agccaaggaa ttgaacacga cggatgttac
    32401 gattaaaaaa gacaataatt ggatttatat cttagtagct tcacttttct tattgtttat
    32461 tttattatgc atacttttgt ttgttttgag aaaaaaagaa gagaaagaaa agtaaggtta
    32521 agataacaaa tagtaaattt ttattattgt aggatggttg ggctcttttt ataacaatat
    32581 aaaatatacc attttattaa taaaaatgca tactagtaaa tgatataatt ctaataacag
    32641 gtttgatcgc gagaaaggcc aaataaaacg ctctccaaaa tgttaaaaaa aagacaggac
    32701 gatgtatatt atacaaacaa gaatcgctga caacagtgac tcggatccag gaaatcagtt
    32761 ttatccccag ctagttggtg aatgcatgga aagtaatata cgtagaaaga gctgatgact
    32821 aatgaattat gagttttttt taagcaaaga agatcacaga agttattcat tacttaaata
    32881 tttagaagca agctcaaatt tatcagagtc aatatcgaat gttcaagaag agttgtcgct
    32941 atcgacattt ctcttgaaaa aaacgatcga taagctgaaa catgatttac aaaagttcga
    33001 tctagagaaa aatttgcgag taaccgtcag tgaattggat atatgtctgg aaattaatgg
    33061 aaaatgttct agcaaagctc tgttatctcg ttatataacg gattctttat ctatgaaaat
    33121 ggtattggct ttctttcagg aaaaatatat ctcagtagaa aattttgcta aacagcatca
    33181 cgtgagttat tctgtggctt ataaagtatt acaaagacta aaaagaaatc taaaaaaata
    33241 tcaaattttt tttgaaaaga gaaagttga
//
LOCUS       DAZUMM010000025        31022 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000025 DAZUMM010000000
VERSION     DAZUMM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.025"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..185)
                     /locus_tag="ACG8E6_001105"
     CDS             complement(<1..185)
                     /locus_tag="ACG8E6_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061543.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
     gene            complement(332..667)
                     /locus_tag="ACG8E6_001106"
                     /pseudo
     CDS             complement(332..667)
                     /locus_tag="ACG8E6_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(679..2271)
                     /gene="mobP2"
                     /locus_tag="ACG8E6_001107"
     CDS             complement(679..2271)
                     /gene="mobP2"
                     /locus_tag="ACG8E6_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFS5061544.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDQEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYVVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNSLTKVVKEPVRYD
                     RHAMNLLNEVYRNLPEKRSEWNYGDENRTKLSPYTREKLDELTQYVLTKEPEYQEYIQ
                     ISKALKEEAKNLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            complement(2291..2713)
                     /locus_tag="ACG8E6_001108"
     CDS             complement(2291..2713)
                     /locus_tag="ACG8E6_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061545.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKSINESVHARVDPFDPTKFLKR"
     gene            complement(2755..3030)
                     /locus_tag="ACG8E6_001109"
     CDS             complement(2755..3030)
                     /locus_tag="ACG8E6_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061546.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            complement(3793..4911)
                     /locus_tag="ACG8E6_001110"
     CDS             complement(3793..4911)
                     /locus_tag="ACG8E6_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061547.1"
                     /translation="MKKVYQKCLWISYITVGIVGFFFIVAVTLGTIFLWFTNEDLLLK
                     GLMSVISCVLLILLGTEINKLLPSRWQEKKKEMREEIEIKSTDWIKHTWLPLKRNSKR
                     KEFWILQLWPLGKLCSYLARFILDLIIYSIVVFGTAYYLLNDPNRIAASDIFTVLMTL
                     IFVMLLSIFTQIVMQFTRQKLTAGKLFKSLFDLIIVNAILATLIKQIEQFGRTTKGVK
                     EFLMTFVHFVNGHMYLFEVGIILFLVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYYVD
                     FGRYDELINTDIVHTSIHEATVKGIRTTLKDGQETKAIDRRKEVYNFYEVEANLRYGI
                     GKLSLNGRNIPQNKPYIGWKSDALEVTKKLPMEEEEIQ"
     gene            complement(4929..5159)
                     /locus_tag="ACG8E6_001111"
     CDS             complement(4929..5159)
                     /locus_tag="ACG8E6_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061548.1"
                     /translation="MKRANLVGKDTFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLDTLKFRSFEALYLAAKKYFTAERK"
     gene            complement(5182..5988)
                     /locus_tag="ACG8E6_001112"
     CDS             complement(5182..5988)
                     /locus_tag="ACG8E6_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061549.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEISTNAFVNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFAVKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKENI"
     gene            complement(5999..6643)
                     /locus_tag="ACG8E6_001113"
     CDS             complement(5999..6643)
                     /locus_tag="ACG8E6_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061550.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNLVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            complement(6659..7864)
                     /locus_tag="ACG8E6_001114"
     CDS             complement(6659..7864)
                     /locus_tag="ACG8E6_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5061551.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKNSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            complement(7864..9888)
                     /locus_tag="ACG8E6_001115"
     CDS             complement(7864..9888)
                     /locus_tag="ACG8E6_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFS5061552.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            complement(10051..10644)
                     /locus_tag="ACG8E6_001116"
     CDS             complement(10051..10644)
                     /locus_tag="ACG8E6_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061553.1"
                     /translation="MRVKGQNSVDRKSILQFGKPEERSILDLFPYKSCTEEGVLITKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(10645..10977)
                     /locus_tag="ACG8E6_001117"
     CDS             complement(10645..10977)
                     /locus_tag="ACG8E6_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061554.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTIGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(11068..11541)
                     /locus_tag="ACG8E6_001118"
     CDS             complement(11068..11541)
                     /locus_tag="ACG8E6_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061555.1"
                     /translation="MEDILIATFITALGAFLTIFVATIKGFKDTESLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTELFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENLNRLNDEMQRVHLENEELRAQNYELIQMKRNLEQKIFELKEEN"
     gene            complement(11602..13962)
                     /locus_tag="ACG8E6_001119"
     CDS             complement(11602..13962)
                     /locus_tag="ACG8E6_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFS5061556.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLENDPNKVEHIPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKIIGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNVAKNVGSGAKNLASAGKSGLSNLNDIRKNGFGN
                     RKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAE
                     DLTEKAKDTLEEKLDGTRAKELAEAVKNPSETLEKQKEKASEVKEGLKDAYGEGQVNA
                     AVKANTTTEKENQEGAAIPSAKDHQDELHSNENSEEGQNGQLPKTDPEQYRYKSNINS
                     ENEKRISDLPKRNQEVQTDDDSIDLPNDNGVEVGTEKSTKNGLPKAQNLPMKDSRLNQ
                     FKENDQSFVHQSTAGNVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAP
                     EPIQKVHTGYKPANVAFTAPTKINVTRDTKNRNNALKEQLLKDIKP"
     gene            complement(13959..16568)
                     /locus_tag="ACG8E6_001120"
     CDS             complement(13959..16568)
                     /locus_tag="ACG8E6_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFS5061557.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPDNEFKQLNALDEFMNQLPPGNTAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHRGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     INSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLRQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            complement(16568..18631)
                     /locus_tag="ACG8E6_001121"
     CDS             complement(16568..18631)
                     /locus_tag="ACG8E6_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061558.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGQLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            complement(18701..19003)
                     /locus_tag="ACG8E6_001122"
     CDS             complement(18701..19003)
                     /locus_tag="ACG8E6_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061559.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            complement(19021..19263)
                     /locus_tag="ACG8E6_001123"
     CDS             complement(19021..19263)
                     /locus_tag="ACG8E6_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFS5061560.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            complement(19260..19724)
                     /locus_tag="ACG8E6_001124"
     CDS             complement(19260..19724)
                     /locus_tag="ACG8E6_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061561.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            complement(19751..21841)
                     /locus_tag="ACG8E6_001125"
     CDS             complement(19751..21841)
                     /locus_tag="ACG8E6_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFS5061562.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKTTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            complement(21838..22098)
                     /locus_tag="ACG8E6_001126"
     CDS             complement(21838..22098)
                     /locus_tag="ACG8E6_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061563.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            complement(22111..22872)
                     /locus_tag="ACG8E6_001127"
     CDS             complement(22111..22872)
                     /locus_tag="ACG8E6_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS5061564.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLFSDQLKLVSNGKTKTIDQQT
                     GVFEFSIEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            complement(22888..23640)
                     /locus_tag="ACG8E6_001128"
     CDS             complement(22888..23640)
                     /locus_tag="ACG8E6_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5061565.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIVAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWTWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            complement(23655..25631)
                     /locus_tag="ACG8E6_001129"
     CDS             complement(23655..25631)
                     /locus_tag="ACG8E6_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5061566.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGAGGITVNGVKSETSPYSVFKWGDIA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGIGIKATPLEGAEFAAKNAEGKYYGGLVDTDKNGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            complement(25684..26354)
                     /locus_tag="ACG8E6_001130"
                     /pseudo
     CDS             complement(25684..26354)
                     /locus_tag="ACG8E6_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
     gene            complement(26436..27509)
                     /locus_tag="ACG8E6_001131"
     CDS             complement(26436..27509)
                     /locus_tag="ACG8E6_001131"
                     /inference="COORDINATES: protein motif:HMM:NF037933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFS5061567.1"
                     /translation="MDGGVKSSYDIIYDNAAQLVTFFVGCLFLLNLYKVKRNDLFFIA
                     AGILFYLFFSFFRSYRVDTFYGDLIVPTIIAIILIVKWTEFTVFDRSLYLVVFYVFLL
                     VTVYRVFTEIPVPEGQSIWNPSNKLSDIWINTNTIGSSLMTLALLITGFASSFVKWYM
                     RLLSLPALILAGATIWVCQSKAALYALVIFVILEILPKKIFRNTHLSFWMYAAVSVLA
                     LPISYFAAMSDKVHLFTGREEIWRKFYETLGEKTGQIFVGMKPFFFHIGNQILGNHNT
                     YHALVNHYGLIGFALSIIPLLSFLWLLVRKCELTNGQLTFLWAFLAIMCQSFMEETLT
                     TISWVPIVFLMLAMATHQYKTTR"
     gene            complement(27604..28293)
                     /locus_tag="ACG8E6_001132"
     CDS             complement(27604..28293)
                     /locus_tag="ACG8E6_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HFS5061568.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGHQKDKIAEKVDGVTK
                     TIHIQEQDKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYPDAEMGNGNYILAGYNVDTPSVLLSDMIDLSEGEEIDLIDA
                     HYKYQYEVSKRGKIYDYSQVVQSKENNSFLSLPKAGKKPLLTLLDFEANNMKKCDVIQ
                     CELVNVEKINK"
     gene            complement(28420..28658)
                     /locus_tag="ACG8E6_001133"
                     /pseudo
     CDS             complement(28420..28658)
                     /locus_tag="ACG8E6_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28724..28963)
                     /locus_tag="ACG8E6_001134"
     CDS             complement(28724..28963)
                     /locus_tag="ACG8E6_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061569.1"
                     /translation="MARPVLDAMDMDTLTKGRYVQLLARSYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWRIYNGIKDLKPARPVVKRKKKITQE"
     gene            complement(28989..29111)
                     /locus_tag="ACG8E6_001135"
     CDS             complement(28989..29111)
                     /locus_tag="ACG8E6_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061570.1"
                     /translation="MKFFEVKSPCYYALIAENNEEECAEITTNNKKLVLSLDIE"
     gene            complement(29130..29471)
                     /locus_tag="ACG8E6_001136"
                     /pseudo
     CDS             complement(29130..29471)
                     /locus_tag="ACG8E6_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
     gene            complement(30090..30422)
                     /gene="traE"
                     /locus_tag="ACG8E6_001137"
     CDS             complement(30090..30422)
                     /gene="traE"
                     /locus_tag="ACG8E6_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFS5061571.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
BASE COUNT         8598 a         6265 c         4766 g        11393 t
ORIGIN      
        1 ggataatctt ctgccgcatc gacgaacgtc ttcgtagtca gatttaacgt taaaatatcg
       61 ccactttgat aagcttttgc agctttgtct gtgactacaa aagctctgcc tgtttgaatt
      121 tctgtcacaa aaacgagatt gtctctgctt ttttcaacaa caactccaat agttgaataa
      181 gccatttctg cttcctccct tctctttctt ttgatataat gtagtcggaa atcgcaccac
      241 tcaatgaatt ggcggaaaac aattcagtga aaaaaaagag tcctggacaa tcacgttcag
      301 gactctttta cttcttttat tcacttaata actaacactc tgtgagtgaa atagactctt
      361 ttatgctttt acatggaaag ttattgattc cctcttcatt catttcccaa gtagtataat
      421 aaggtgttaa cttggcctca tattcttgaa tgataaaata tttttcaccg ttcacatgat
      481 tacaacaata cacaccagta gctgggtact caacgactaa aaattaattt ggatcaatct
      541 gttcgcttct caacatttct cttatctgaa acaaatgctc tttttccaaa caatcgggaa
      601 gttgttcgat tcgttctttt tttagtgatc cttctatgac tttccattga ttcaaatatc
      661 gtttcatttc tcttcctcct actgtctttc ttgttccatt tgaattcttc gttgttgctc
      721 ttcgtatgct ttacttgcgt ggtatttttc ttcatgggca ttaatcgttc gatttaattg
      781 acgcaaactc cgctgcgtga caactctttg ttggaattgt ttcctgcgct tcatgaattc
      841 ttcgtaacta tatttgtatt cattcgttgg caattgttta agcgttcgaa aggatcgttc
      901 attgtaaata gattgcatct gatcaacact ttctattaat ggttttattt gactatcctg
      961 tctcttcagc tctgttagga tggcatttcc tgtgcgcttt tttaaatcaa agagagcatt
     1021 tttctctgca tcctttgatt ctctcttgct ttccccataa agattttttg cttcttcttt
     1081 caatgctttt gaaatctgta tatattcttg gtattcaggt tcttttgtta acacatattg
     1141 ggttaactcg tcgagtttct ctcttgtgta tggtgaaagt ttggttcgat tttcatcccc
     1201 ataattccat tccgaacgtt tttcaggtaa gtttctatat acttcattta ataagttcat
     1261 tgcgtgtcga tcataacgta ctggctcttt taccactttt gttaagctgt tacgcaaatc
     1321 actttttcgt gtcaataaat ttcgtttctc cataccaatc attcgatcct tttcataaga
     1381 gaaacgttct aactcatgca cataattggt tttcattgcg tctaatgttg cttgccttct
     1441 ctttcctttc ggtacaacat aactcacacc attttcaacc tcagtgtaag gtttacgcgt
     1501 attttctttt tcaacaactg caaaatgaat atggatatgt ttagtgttac ggtgaatcgt
     1561 tgccatccag tagcctgttt cgttcagttg ctctttctcg aataatgtct ccatcatttt
     1621 tcttccagct gcttttaacc cttcttcatc taaagtatct gttttgggat cgtacaatcc
     1681 tattttttca agatacgaat tatcatgaga gacaacatct tgataaatga ttgagccttt
     1741 ttgttgtcct ctttggaaga tttgcttaat ctccttcaga tcacttcctt tcacttcgtc
     1801 tgaagccata gaaaaaactc caggaaggtt tcctgaagct tttttgtgtg gctctgtttg
     1861 atattttttt attgcttggt tcaagcgttg gtattctttt tgttcccttt gcgtcagttc
     1921 tttcttttgt tctaacgcat agtgacgggt catataagaa agatatttgg taaaatcttg
     1981 ttcctctaac ttgtcaaagt tgattccatt tcccatcaaa gattgagctt cattcgacgt
     2041 ttcgctgaat tcttgatccg tcttactctc cgaaaaagtt tctccttcag gaatataaaa
     2101 atcctctaaa gcattcttga ttcgttgcag ttcctgttct tcttcaggcg tacgtcgttc
     2161 catttcttcc agcgcttttt ctcttgtcat ataatcaatg tatgttgaaa aagatttttt
     2221 attgggcaag gtaaattgac aagtgagaat gactgcaggc gatttattca tcatgttccc
     2281 cttctacttt ttatcttttt aaaaactttg ttggatcaaa cgggtctact ctggcatgaa
     2341 cagattcatt tattgatttc ttgattttag gagaatactt cttcgtttga ttcgctttta
     2401 ttttcccttc cacacgctct tttgcttctt ggtaaacagt tgaatttgta ccaattaaaa
     2461 tccctgtatc tttcaaatga agatccgcaa actctgaaac catctcggtt aaaatagaaa
     2521 tttcttttcc cattgcattc aatttaattt gaattggatt tattttttct tcgatttttg
     2581 gatcatacat catacacaaa tgtctgactg cttcactata attagatata tcttcatgag
     2641 tagaaattat ttcatcaagt acttcgatat gcatatcgta aagatataca tgttttcttg
     2701 ttgcttttgc caaaattgat ttcctccttt tataaattca caatactatt tttattattc
     2761 aattaacaaa ccaattaatt gattatttgt tgcaatatat tcttctaaag tttttgtatt
     2821 ataagtttga atatccacaa atttttctaa caaccgatca aattggtctt ttttataatt
     2881 cagaagagcg tgatcaaaat cattttcgat caaaactttt aggtattgat ttcgactcca
     2941 ttttttgcca gtcttgaccg taagttcttc acaacgtttg tcaattttat taatgtactt
     3001 ttcttcaata tttctaatta ataagtccat attttcctcc aaatttatta tgtgtaggga
     3061 tatgtgtagc tacacatatc cctacacatt ttttgatttt gctacacaca aacgcttatt
     3121 ttactgccca cattcgcttt catcctaccc acaaatctac acacaaatcc aaaattttga
     3181 tatcatttat ctttcttaga aacgtggtag gcacagccta cctagcacct ttcgtaagaa
     3241 agtgatatca cgtttcctta tgctctttaa aaattaaggt tatttgtgta tttagacaat
     3301 aaaaaatcct tctaactttg tcgattagaa ggaaatattt tgtcatatga aaaatgaatt
     3361 taagaaaaag tgtatgctat aattgatacg ctgtcttcct gtttggttga aatcttcagc
     3421 cagaaaggag gagtgttaaa tgttttatgt gcttgatgtt tgcgttagaa tcgtggttaa
     3481 tgttttctcc catctcatta acaaatggtt agattctaaa catgataaag acagctagcc
     3541 tgttcattat ttggttaata gtgaataaag aaaagagcct tacgcagctg caactgctag
     3601 ggctcttttt gagtattaaa tgttttatgt gtttgcatta tcagtatagc atacactttt
     3661 ttcacagaca actttctaca ttttttcttc ttttttcaat caaaataaac taatgaataa
     3721 tataaactct tattgagaac tgttggaaat gattactgat atcttcaaac aaatgattca
     3781 aattgactag actcattgaa tctcctcttc ttccatagga agctttttag ttacttctaa
     3841 agcatcactt ttccagccaa tgtatggttt gttttgtgga atatttcttc catttaacga
     3901 caattttcca atgccatatc ttagatttgc ttcgacttca taaaaattgt agacctcttt
     3961 tcttcggtcg attgctttcg tctcctggcc atcttttaac gtagttctga tgcccttaac
     4021 ggtagcttca tggatactcg tgtgaacaat atctgtattg atcaattcgt catatcttcc
     4081 aaaatcaaca tagtactcat aaccaagaag tgtcggaatc aagtcaatct caatttcttt
     4141 ttcaattgaa cgtttaataa gtttcgtgga tacatacccg actaaaaaga gaataatacc
     4201 cacttcaaac aaatacatgt gcccatttac aaagtgaaca aacgtcatta aaaattcctt
     4261 cactcctttt gttgttcttc caaattgttc gatttgtttt attaaagttg ctagaattgc
     4321 atttacaata attaaatcga atagactttt gaaaagtttt cctgctgtta atttttgcct
     4381 ggtaaattgc atgactattt gagtaaaaat agacaatagc atgacaaaaa tcaacgtcat
     4441 tagcactgta aaaatgtctg atgctgcaat cctattggga tcatttaata agtaataagc
     4501 cgttccaaat actacaatcg agtaaatgat taaatcaagt ataaaacgag ccaaatagct
     4561 acaaagttta cctaatggcc ataattgtag aatccaaaat tctttacgtt tgctatttct
     4621 tttcaaagga agccaagtgt gtttgatcca gtcggtcgat tttatttcaa tttcttctct
     4681 catttctttc ttcttttctt gccaacgaga aggcagaagt ttgttgattt ctgtccccaa
     4741 caaaatcaat aggacgcatg agattacact catgagtccc ttcaataata aatcttcatt
     4801 agtaaaccat agaaaaatcg ttcccaaggt cacggctaca ataaaaaaga agccaacgat
     4861 acctacggtg atatagctga tccataaaca tttttgatac acttttttca cataaaatcc
     4921 tcctgttttt attttctttc agccgtaaaa tattttttag cagctaaata caaagcttca
     4981 aatgatctga attttaaggt atctaaactc tttctaccat tcttcaactc agatattgtt
     5041 gtgttagata aattcacttc ttgacatatt tctttagctt ttacaacttt gagataggct
     5101 ttaatcaata ctttctgctc agcgaaagta tctttgccga ccaagttcgc tcgcttcatt
     5161 tcttttactc cacctattct tctaaatatt ttcttttgtc atacgatcaa ttttttcttg
     5221 aagtttttga atgatctctt cttgatcttt catttgcgtt ttcgatgttt caatttcttg
     5281 ctggtaacta ttaatcttat tttcagtatt ttcctgatcg tctgcaactt gataaggcaa
     5341 ttgatctttt agtttcttca ctaattcttc attcaaatca atggttgctt ggaaggattc
     5401 aatctgtttt tgtgctgctt tttgttcttt tttgtatcct ttcactttgt aattgatcgc
     5461 atgttgttca taatcttcat ctttcacttg atctttaacc ttatcttcgt gaacgtaata
     5521 aatcagatct tgcggttctt gcatatccac ttttggatca attttttcag gaataatggt
     5581 taaacgcatg gcaatatatt cttctggtac atgagatagc ttcactgcaa aataatcatc
     5641 agacactttg atcactttgg cttttattgc ttgataatcc cctttagctg tctttacttc
     5701 tacgcgataa ttaagattag ccagttcttt taataagctt tgatcttcat cgtcgattgc
     5761 aaattcaaaa actaattgct ggttcgctcc tttttctttt gaaaccatcg tcagttcctt
     5821 cgtgtttaca aatgcgttcg tgcttatctc atctggtatg tctaccttct tatgcgtata
     5881 aattggacta gcaagaatca aaaagccgag aatcataaag atccccagcc atctaagata
     5941 actttttcgt tttttgaaac gttgtcttgc tccttttttc ttactcattt tcttcctcct
     6001 aacgagtgaa ataactaaag tctgtgactt tccattgacc atctacttgt ttttcagtga
     6061 cagttaatcc accttttatt ttttgcggtt tgacatcttt gaattgagga ttgccgattg
     6121 tataatcaac aaatacgaca agattttgtt cctcttttac actgcttgtt tgagcgtaag
     6181 tttgcacgtc acctaatgta aaggagtaac ttccgtaatc atcatgttgg tgatcatcgt
     6241 ctgcaggact taatttgtcg atcacttctt gggttgcgac atcttttagt ttttccgtta
     6301 attcatttct gcttaatgat ttatcaatgg ccaattgacc gaactcttct gccgtctttc
     6361 tttgtgcttc agttacttcc tcttttgctt ttgtgccatc ttgttgcttc agctgatcga
     6421 tttcttggtt tttctcattg atggtttgat ttaacttgtc gatcttcgcc tgtgcctcag
     6481 aggaattttt tttgagcttc tcagacgcgt ttttagccac ttcttcaatt ttaaaggtat
     6541 ttgtctcttg ttcttctttc agctgccaaa acaacgcgat attgccgatt aaactaacgc
     6601 taattgcagc tgttaagacg atttttaaag ttttattttc catatttttt ctcctgatct
     6661 attttttgct gctattcaaa aatggtaatg gatcaatcca tgtaccatcg tctgtgaatg
     6721 cgtgtccttg tgcttgacgc caatccattc gagtgaaacc taaatgcaag tgtgctgtat
     6781 ctcgtatccc gattacttga ccaactttca cttgatcgcc aaccttcaca cgagaatttc
     6841 ctgtactatt cgcaaattct tgatatacca tattcaaacc gtcgtaatta atcacgatca
     6901 cacatgcacc taaacctgaa ataccaggat tgccaacgta cacgactttt cctccatgca
     6961 ccgcgtgaat ttcacttcct ggatgatcga ctgaaccaaa gtctaatcca tcatggaagc
     7021 cattcggacg gaattcaccg cctggatttg ttccaaataa ttgaccacca ctgaaggaat
     7081 ttttatctaa actgctgcca gggaatggat tcgtccattc accagtgatc gtgccgttgt
     7141 tattgccacc ttcaccccaa tgtttgatgg cgaattcatc atattcggtt aatttattag
     7201 aagtgataat ggacatacat cctgtataat acggactatc ttccgtaatt cctgcagcgg
     7261 cataaccgcc tcgtcctaag atcaataaac ttgcgccagg gcttttattt cctacacatg
     7321 cattaaaatt ggattgtgtg gctaccataa agccgtaata tgagaaggca gcttttgagt
     7381 tgggaaactt catgtaccag ccaccttcaa tgccaccaat tcctcgtgca gttcctttag
     7441 gaaacagaca accatcaaac caagtaatcc caaaatagtt gagatcattt cttgcggacg
     7501 ccgacgtacc aaacgctgat tcataaccgt attgcgaaag gatcacactt ggtaataatt
     7561 tatatttcac tgctgcacct acagcatatt gcgcaatctc atcagaaaaa gggcctcttc
     7621 ctttgatttc tttataaatc ggtaagcctt ccgaatattc tcctgtaaaa gcttcgccac
     7681 cactacttga atctgaatca gaattatcaa tcgaacccgc cgcaagtatt agaaaaaggg
     7741 tcacgaacac agtaatcaaa atagtaatca aacctgcaga agtaccaaac aaccacaaaa
     7801 ataagcgtcg ctttgcacga cgcttaacat actgcttgat taaactttca ttttggtttt
     7861 ccattatttt cctccatcaa acaattttaa ttgacgtttg ttcggactaa aatgaacacg
     7921 aatggtttct tttgagccaa aattgatcac agcgtctcct tgattttgtt gaggaatgga
     7981 ttctaattct gagtttgtca ttgatttacc aactaatcct tctatatgac gtaaaataga
     8041 ttcatccata cgcataaaga tcttcgtaga agaaaattca aagactttct tcagtttcga
     8101 gaagttatca gaagacatat tttctggcac catttcttgt ggggattgtg tagctaaaac
     8161 agtcattgct tctactttac gcatttctcg ttgaaagttt gtgacataat ttaccgtttc
     8221 aataaaatct ggattaatcg cattatggca ctcatcaatc atcgccaaga agcatttgaa
     8281 gtcttgttct tccatttgtt tttctttaat taaacgtcgt tgtcttcttc cttgaatcaa
     8341 tgcctgattc caaatcaacg tcatagccat gtacatttga cattgttgta ccccacgatc
     8401 taaacctgta atagagtcaa tgtcaaaaaa gaccaactgt tgttggtcaa ggtgtcgaat
     8461 cgtactatga ccatcaaaaa tcatgccata cgtctcacat aaggattcta cgatggattc
     8521 catttcttct tttcgattct ttttctcttg ggaagcacct ggtaattcca gttgtcctaa
     8581 aaagtctaaa aattctttga acgtcggata agcttctgga tctttccctg tgacttgcgt
     8641 ctccgtattt gaacgcgagt ttgaggaagt gaaaccgcgt ggtactgttc catactcttc
     8701 ataaaaacga tttaaatagg tcttaaattc tgctttcatc gttcctttta gctgaggact
     8761 cactaactgt acctgataga ccactttgtc taagtgcgtt tgataggacg ctgcttcatt
     8821 gatcttcaaa tctttgactg atccatcgtc atataaactg gaagcttcat catacgtacg
     8881 tgtggcaaat atttctaaag gattgatcat cccgtcagat ccgtctaaac gcaccataat
     8941 gccgttatat tcctttaaaa agggaataaa gtcttttccc ttttcaaagc cccaaataaa
     9001 gtctcctgca gctaaatggg cttccgcgat cattttaaga acggtcgatt taccagctcc
     9061 catcattcct agaagcaagg tattggtaga caaccgcaca ctatttttat acgtggcatt
     9121 ccaaacaaac gggccgcctg tcatggtttc cccatattgt gttccacctg gatcttttag
     9181 cgtttggtgg tggaacggga aacctccgcc aatgtttagc gatggtagtg attgcccttt
     9241 gatatttcca aaccattcag cttgttcatc gaacatggtg aacatcgatt gccagtctct
     9301 tttcgttttg tgcagatagg tcactgcacg ataatccatc cctttgagat tttcgatcag
     9361 ttcctgtgct cgttcatcta cttcttgaat cgtgtttccg taaacatgta ctctcacttt
     9421 gacttgttta gaaatttccc cattagaagt caattttcgg gcatattccg ctagttctag
     9481 gtattcttcc gcgcttttat ttcggtcagt cgcttttcgt tcattatctg accgatcatt
     9541 taattcatct aaggcacgat tgatttgatc aatcactttt tgcttttcat ctgtgctaac
     9601 atctagtgtt gccactgtat gaggattgtt catgagattg gccaaccaaa aaagcgtagg
     9661 attttttgtg tagtgataaa ctgtcacaca tcgtgaataa ccatttccat aggtcacata
     9721 ctcttcatcg aaatttaatc caccttgtgg catcacttgt tttaaaaagt ctaaatcata
     9781 ccctttggat tttaaaattt ctatttcccg atttcctagt tgctttaatg gatcaagtac
     9841 cttttgatca ttttgtggtg aaaaagctgt tttaattgct tctaacatgt acagtcacct
     9901 cttcttgttt gattctttac ttggttaaaa gattttcctt ttcccaactt acccaggctt
     9961 aaagtgtgag gaaattttct agtggtctct tctttcggaa catctttttc taaccatatc
    10021 aacggatatt tcattctctc ttccccattc ctactcatca ttcatattat tcattcgata
    10081 aagaatcttg atcaattctt ttttgtagag tggttttaac cctaaaatgc cactagatcg
    10141 cattaactga cggtttctgg tttgcaaatc cttgattgtc tttccttcaa tgacgaaaaa
    10201 gaagcttaaa tcagggcgtt cctgttctgc tcgtttcaat tcctggattt cctctactaa
    10261 tttcttttca tattttctta atcgctgtag atttcttcct aaggactgtt ccatacgatt
    10321 tttatctaat aactgccgct tttgcacgat ttgctccgtt agatccgttt ctgtggtcaa
    10381 actaactaac ttaatcgttg gcacatacgt acgcatgaca gtggtcaact gattcattcg
    10441 ttccagtttc tcttgttcat ttaatccttc cacatctgta gaaactacac gataataacg
    10501 ttgaaaatga tcttctttag taattaagac tccctcttct gtacaggatt tataaggaaa
    10561 taagtccaag atagagcgct cttctggttt tccaaactgc aaaatggatt ttctgtcaac
    10621 tgaattttga cctttgactc gcatttattc atcctcctta tacgtaccat gataatttcg
    10681 gttaggatct aacgcatgat aatggttctt gtccgtaaaa aaaatagact cgataatcgc
    10741 aaaaatctgg cgttgacctg ggttattata gggtcgccaa acaatcacta aagctatcgc
    10801 aatccataag ccataattca agagtgcaaa aaagagtggt aagtggacaa gattattgac
    10861 tattttcgcc caaaaaaagc aaccaccaat cgttaataga tctaaaaaga aaaaggcgaa
    10921 aaacttccat tccccactaa atcttggtgg atttttcata tgaatgtatt tatccataat
    10981 gtaaccatac tccttatgtt ctttattttt agaaacaaaa aacactgtga tttctcacag
    11041 cgtttttcta aatattataa ctctaattca attttcttct tttaattcaa aaattttttg
    11101 ttctaggttt cttttcattt gaatcaactc ataattttgt gcgcgtaatt cttcgttttc
    11161 caaatgaact cgttgcattt catcgtttaa tcgatttaaa ttctccaatg attcttgtat
    11221 cgtttcttgc ctaaaattaa gtgagccaag ttgacgatct ttttgttgtc tttgctcttt
    11281 caattctgac gatatttgct caacagaatg aaatagctcc gttaatttta ctgtgaatgt
    11341 gggcatagca tctttcaaat aagtgaatcc ttctgaaagg tgtacaataa tgctttcggt
    11401 atgtgttttt tgaccatcta aaatagactg atgatctttt tgtaaactct ccgtatcttt
    11461 aaatccttta atagtcgcca caaatatcgt taaaaatgct cccagtgcag ttataaacgt
    11521 agctattaaa atatcctcca caattttctc ctttccattt taaacttgtt ttataaagaa
    11581 aaaggccctt aaataggcct tttatggttt gatatccttc aatagttgtt ctttcaatgc
    11641 attgttcctg tttttggtat ctcttgtcac atttattttt gtcggtgcag taaatgccac
    11701 atttgctggt ttgtaacctg tgtgaacttt ttgaataggt tctggtgcag cttctcttgt
    11761 cacaacattt tgaacggacg cattccctgt ctccccactt tcagataaat tgtaagaaac
    11821 agttgtcaga tttccatttt caggcaaacg acttggtttc atttgaacat tacctgcagt
    11881 tgattggtga acaaagcttt gatcattctc cttgaattga ttcaaacgac tatctttcat
    11941 tggtaaattt tgtgccttag gtaaaccatt tttcgttgat ttttcagttc caacttcgac
    12001 cccattatca tttggcagat cgatggaatc atcatccgtt tgtacttctt gatttcgttt
    12061 tggtaaatct gaaatgcgct tttcattttc agaattaatg ttagatttat aacgatattg
    12121 ttctggatct gtttttggta attgaccatt ttgtccttct tctgaatttt cgttagaatg
    12181 caactcatct tggtgatctt tggctgaagg aatcgctgca ccttcttgat tttctttttc
    12241 ggttgttgta ttagctttta ctgctgcatt gacttgtcct tcaccatacg catcttttag
    12301 tccttctttg acttctgaag ctttttcttt ttgtttttct agtgtttcgg atggattctt
    12361 cacagcttca gccaattctt tagctctcgt gccgtctaat ttctcttcta aggtgtcttt
    12421 ggctttttct gtaagatctt cagctgttcc ttttaaccca cgttcattga cgtaaccaag
    12481 accttctgcg gctttagaaa caccattttt cgctttcccg aacatttcgg aaactgtttt
    12541 cttactatta ggtacagttg tagctttatc gtctgattgt tcttccccgt tatcaggggt
    12601 aacatctttt cgattaccaa atccattttt acgaatatca tttaaattgc ttaaaccact
    12661 cttaccagca cttgcaaggt ttttagcccc gctacccaca tttttcgcaa catttcctaa
    12721 ggcaaacatg ctaagaagag aattgctgcc gtttttcaga ctcgtatcaa cgccgaaaat
    12781 tttcgtaact gattgagagc ctttgaatag cgcaatggtt aatacaatta aagcgacaac
    12841 atacaagaat ccaatgatct ttcctgccgc atgcaaatca ccgatacctg aaagcattaa
    12901 tgtatagatt cgtaattcta aaatcgtaaa tgccaataac aagaaggatt gagaaatatc
    12961 ttctatagca cttttcatcc gttgtcctgt ctcaaagtcc gttgacgcgg caatcactaa
    13021 atagaatttt ttaaatacca gctcaataat gcaagtaaca atcgtaaaca agataaataa
    13081 ataggctacc gacaaagaag attcaccagc taaaataata cctggactac taatgtatcg
    13141 gcgatagcct gaagtataca catattttgc taaaccttca tcactaattt gaacagggat
    13201 ttctttccca ttatcgtcta actctagtcg atactgtaat gcctttaacc gatctttttg
    13261 tactgtacta atatcacttg attcagacat tttcttagct tgatcaggtg taataatccc
    13321 attgatattc gctgttttaa aatttttcgc cgtgagagca tttcttttgt ctgttggtat
    13381 gtgttctact ttgttcggat cattttctag taacgtactc acatcaatca cattattttg
    13441 aataatttga aaagcaggtg aaccagtttt atctgcttta tttgctgcag tcacgtctcc
    13501 gtaaaagttt ttactaatat ttagcccttc atcgattaaa gaactaccac caaaaatcaa
    13561 aatcatggcc ataaatagat taaccaagat atttttgatt tttggtggat gtttactcat
    13621 aaaaaatttt aagcctaaat aaattaatgt gacgacacaa aaaagagcca ataccttact
    13681 aatcaaactt tgaaacagtt cattaacacc agcggcgttc aataagtctt ttaaatcaaa
    13741 gctttgataa attgtttttt ctagtaaagt attgagatta tataaccctt taatcaaacc
    13801 ccataaaaaa gtattacaaa tccaactgaa tacatcttga tactgcagat aattaccata
    13861 atcttgtaag actttcgtta cggttttggg atctgcatcc ccatccccaa ataagcctga
    13921 ccaccagtcg gcaatgccgg aaaatattcc tccaatgatc attctttcac cgccttttca
    13981 gcttctagca aaagtcgttt ccttataacc tctgtaattt cttcattttg acgcaatttt
    14041 tcaaggtatt cttgcaaacg atctatttgc ttctcttcat aaaattgaat aagtaacttt
    14101 tgtgccgttg agttgtctaa agattgaacg attttaataa aaatgttttc tttagcttcc
    14161 tcttctattt gatcgtgcat attgtgataa agtcgacgtg catagatatc aggtagaaaa
    14221 taatcgtaat caatttgtaa agccttaaga tccaagtccg catgtgtaca atgtatgtct
    14281 atatcattga tatcccacga tggattgaaa tcttcaatat attgataagc ataaggaaga
    14341 ttcgtttccc catgattaaa aataggatac ggccgtattt tatgaccttt caaatcttgt
    14401 cgttttaaag gagcgagtac aatcgtttca ccttctaaaa aagtagaaat tctttcagga
    14461 gttaagatgc gatctgaatc aacattgcga ttgatatgag tattcatacc taatgaattg
    14521 atggtactgt tctcttgttc aaccgtctta ttccccactt ttttggaaaa ttcttctatg
    14581 gtattatcgt ctgtcgacat aatataaata tgtgtctgac aattttcttt gataatcgct
    14641 gcgttttgtt ccccatactt cgcataaagt tgctgatagg attggataaa caaatcccac
    14701 aacatgtttc taccagctgt gactgtcatg attccttcca tattatccat tggtggcata
    14761 ttcccaaatt catctaaaat aaaatgaatc ctacggtgac atttatctcc tttagtctta
    14821 acacattgcg tagataattc tttatacaac tgactaagaa aaatactaac aatgatatgg
    14881 ttagatggat cataatcagg tgtgagcata aaaatagcca ttggtttatc ggtatagtgc
    14941 atccgaatag actctactaa taagttactt ttatcctcat ctaatgttag aacggcttct
    15001 ttttctaatt ctggcaaatg ggtttctcct cgtttttttc gataaacgat atttccctct
    15061 tcgtctttca atactctttg agaaaactca taccaagcaa catattcttt tcctcgatga
    15121 tcgtatttta agcaaacatg atctccctgt tctaaatgac aatcaaagtt aatttcacaa
    15181 aaacctctat agcctacttt gacacgttcc tcatgaaatt tttttccttg atgataaaac
    15241 tctacaacaa ttcgctggtt taacagactc tcgtgtagtt tgagatccaa atacttcgga
    15301 aaaccaatgg acttataatc aatggttgat tgagaagtca ttttcgcaat tttcgtcagt
    15361 gtaaatggat ttagtcctgt gatcgctgtc gatagaatgt tgcctttcgc tttatcagaa
    15421 ccaatttttg ttgccccata ttgttttttt gctgtgtttc caggtggtaa ctgattcata
    15481 aattcatcta aagcattgag ctgcttgaat tcattatcag gatcttggta attaaaacca
    15541 ccaatctcag aaagaagttc gacgagatta ttcggtgtta atttggagta atcccctgtc
    15601 tgatccgctt gttccaataa cgcaaaaata agcgcattga cagctttttg cgcattttcg
    15661 ttaaagtatt tattgtctga agcgttggca tcataataaa tcgtattcgt gaaagtattg
    15721 gttaactgtg tcgctgtgtc tatgtctcct cgcttccagt atttcacgat gagtgccaat
    15781 ggattatcac tcattgattg cagtggatcg gtgatattaa atgcatacac atcataccct
    15841 ctagcttcta gttcatcttt actagcaaca tataattctg tttttggatc attaacaacc
    15901 attgaacttt gtctttctgc acgagacaaa ttgtcaatgg ttgctaaaac ttctatctgt
    15961 cctttccctg aacgggaagt accaacaata caagtattta ctgtatccgt ctgaatatac
    16021 cagtactttt tataatgagt aattggtatt ccgccataac caggaaaacc tccctttgat
    16081 tctggtattt gcggataaat ttgttggatt tcctttaacg tttttaatcg gttatctcct
    16141 ttttgcccat ctgctagagg acgaaaccac atgtccattt tcaatcccat aagtacagct
    16201 ggaacaacac aaatcgtaga aacaactaaa cgtgtgccta aacttgttgt tggggtcaaa
    16261 ggattccaaa aaccagttga atcaaaatgg aataactgcg gcatttcttg taactgaaaa
    16321 atattcactc cttgtaaact tcctggaata atctcaaatc gatgaaatgc ttctggtatt
    16381 cttgtaacaa catagtttaa aggaagataa gctaagccac tataaaaaat cttttgagaa
    16441 atacgtttgt catgtttttc tggtagctct tctcctgaag gagtatagcg atagaaatga
    16501 gaaagatacc tattcttttc taaattgaat cgtttgtctt ttcgtctcgt tgtttctcta
    16561 acacgcatta tttcacctca tttgattctg aactttcgga agtgtttgat tgtacagttg
    16621 aatcgcttga tccttcttta gccttactag gatcactctc tttgctattt tccatacttg
    16681 attctgttgt tgactgattg gtacgattca ataattcttc taattgttgt tcatactctt
    16741 ttcttgcttg ttcttgggct tcttccttga ttgacgtcgt tgtggccaca gaatcagcct
    16801 gtgaagattg attgatatga ttaaaagcaa atactgttcc tccacctaga agcaagctta
    16861 aacttgcaat acatcccacc atccatccgt taaaggaacg ctgtggtgaa ggagttagat
    16921 ttgtttggtt gttcattggg gtttgaacag cagctactga aggcgttggt tgaggttgtg
    16981 ctagataatt taactgagct tgtcgcaatg tatccatttg ttcttgacgt tccttacgag
    17041 cagctgcttc tttttccttt tcttggttta accgttgttt ttctattgcc atttgttgtt
    17101 ccgcaagatc ctgttcacgt ttttttagtt ctaattgttc gcgctctttt tctttttgcc
    17161 gttgtttttc ttcttgttct ttcaattctt gttgttgaat ttcatactct tttttccatt
    17221 ttggaatttg cacttgtaat tcttgctgga tcttttcgtg tttttccata agaacttcag
    17281 tcgttgtttg atatagctta ttcagttgtt catttaattc tttgctaatc tttagcttat
    17341 ctgtacgcaa acctcggtaa aattcttctt ttcgttcttt aatacgttct tttaacaagt
    17401 tttctttttc tttcgataac gcttcttgat tcgtctgagt aaaattattg atctttgcta
    17461 cacacaagga atacgcattg tcgcgtttct tttcgtatcg ctctttaatt ttttgtactt
    17521 cttcttcaat cactcgctgt tcttctttat ttagttcctc ttttgctttc tcccaattag
    17581 acgactgttc cgtcgcatac tcattaattt cttgatctgt ttcttgttga cgttcttcaa
    17641 aggtggctaa taaatcttct tgtgcaagct catgccaatt tgttgattgg acagactcta
    17701 actgttctaa aagtgtgcct ttaccctgtg agattaattc ttctttactc tctaaaaatg
    17761 cttgataggt ttggggtaat tgatatttcg aataaagctc atttaaaaat aatttgtttt
    17821 tttctttcat ccattcatgc tctggcggat attgatccaa aatataatca aatacagttg
    17881 ccttttgtat ctctatttcg tttttttcta cttcttcact gataagtggt tcaatatatt
    17941 cctgagaatc cccttcagtc catgttgact cttcattagc ttcaactgga tcaacttcta
    18001 cttcggccgt ctcttcatcc tccattgttt cttcaaaggg atctacctcc aattgaccca
    18061 tcacatcgtt ctcttcctca aatatcgatt tctgagtcgg aatatcagct tgttcgtcaa
    18121 catagactgt ctctttttct tttgactcct tctttggaaa tattggtcgt tcccatggta
    18181 cttcttcatt tacttcgact tcactccctt gattcacttt tctttggtcg ctctctaact
    18241 cttcttctaa aacttgtaaa agtttttcgt ttcgataaga ctcttccaca ctcatagttt
    18301 catccgtttc tttaaacaca tcaaggcgga ttaaatccat caatgtagga tagaaacctt
    18361 ctgaaatttg ataatccagt tgcccaaaaa cgtattctgt accagctttc tctatttttc
    18421 cttctgtgtc atgtaacgtg agagtcaact taacagaaga tcgactttcg tgttccagta
    18481 ctttccctaa cacatgtagc tgttcttccg tttcaatgac agaatattta attgttttcg
    18541 gtaaaaaaga agtacgcttt cctacttcct tcgttgcttt ttttattaac tctttatctg
    18601 ttttggtatt gcctgcaatc gtaaagtaca tgagtattct cctttcataa aaaaggagta
    18661 aaaagtatcg actacttctt actcctactt ttttactttc ttataatcct tgaggtgttt
    18721 gtccaaacaa tccaaataac gaaaccacaa tagaagctgc tgctaaaata actcctaccc
    18781 ccatcaatac atagcctacg tgcatcttcc cattttcagc tcctcttcgt ccaaatagga
    18841 agaaaagacc cccaataaca atggaagcaa cggccgcagc agcagcaaaa atcatcagat
    18901 tgttttttaa actagttgcg gcagtttgta atgctctaaa aatatcaacc gcagctacta
    18961 ctttcggttg tgttaaaaaa ttcattgtaa ttgattcaaa cataaaattg ttcgctcctt
    19021 ttagtgttta ataatttcgt cgaaaacaag cttttcacgt tcacgttgat agatatatcc
    19081 ttctaagtcc ttgtcttttc cccgtaatag gtcaaattgt gctttttcaa ggacttcttg
    19141 tttccactgt gtttcagata caccgcggcg ttctaagatt ttagacgcgg ctttttcaaa
    19201 tggcgtttta gcttttgcca ctgtgattcc tccttcagga caaataaaaa gactagcaat
    19261 cactcgctag tcttttgtaa cttatattta ttggattgtg atccatcgtg aatcaatttc
    19321 tctttcgttc gcttttcatc cttgctagat ccaagaaaaa ttattttttt tacgccattc
    19381 ttctcatagg gaacaacaat aaataaagaa tcagatttgt ttataaattc cctaacacca
    19441 gctgaatcta ctattttgta tctttctaaa cctttatagt ctttttgcct cttgactgtt
    19501 atttgtacag gatctggatg ccatacactc tcagagtatt cccaggtagt ctcgtctata
    19561 attttcaaat tataaacaag accacctact tcttctttcc acatagttcc tgatttcaat
    19621 acgccattat cttgattagt ttgtttattt ttttcattac tacatccgac taaaataaca
    19681 atcatggcaa tcaaggaagt aactactatt attaactttt tcattttatt cattctttcg
    19741 tttttttatt ttaaatgtat ctcagaaccg cataactaaa agttgctaac acaagtaata
    19801 gtaagctgac cgctgaaatc gttactaaat gtgtagaacc tgttttagga acaatgacct
    19861 gtggcaagtt aggatcaacc ccaattgtca caacatttgt tttattagaa acattatcta
    19921 ctgctactac ggcttggtta tcaatatttt tttctgattg taatggagtt ttagctgtaa
    19981 atatcagctg aatttctcgt ccatctaaaa gattgttact taagaagtca gcggaagctt
    20041 cccacttcag cgttcttgaa ttgctatcat aggttagtgt gccttcagaa gtaatatctt
    20101 ttcctttact cttatctatt acttgcgcag aaccttcttt atatgtcaaa cgagcttcaa
    20161 ttggatcttg aatactaaat tgtttgtagt gatctgacgc agcgtctacg ccttttgtgt
    20221 gccatttttg tgtgactaga tattgtactt cagatcctgc gtttaaagga gctgtcggcg
    20281 tacccttctt accgtcattt cgtccgttta catcttttcc attttgttgt acttctttca
    20341 ctggaatttt agggaaataa acggtaatga tatttgtatc cttttctact ttattaatga
    20401 tcgacgttcc ctgatttttg gctacactat tttgattggc caattcattg acttttacat
    20461 tgatcactaa ttcgtaagtt tcgccgttat acttcattcg attttttaat gtatcagctt
    20521 tcgctgcata ttttacctgg tgttttgttt tgtcataggt cacttcacca gatacatcga
    20581 attttttatt tgtaccactt tcaacgtgtg catcgacgta atttacttct tttggtaaat
    20641 tatcgattaa ctcaaatcga tcgtaaaccg ctagtaaatc cactcctaat cgattcacct
    20701 tctgtttgat tcgatattga atcgtgtctc ctacttgcaa cattttatcg ttcacgttat
    20761 ttccattttt atcaacccct gttttttcag gttgatcagg aacaacacta aagattgtcg
    20821 cagaagataa agtaaaccag gtttgaacat tagaagaacc aatcacaaac gtcggatttg
    20881 cttcagatat tttaaaggac acagtacctt gaccaaactt cggatctcct aatttatcat
    20941 gcaaattatt gaaatcatta tccgcttgtg tcagcttaga gcttcctcct gggtggccac
    21001 ctaccacgtt cagattgttg tagaaagatt taaattctga aagaaccgta tcttttctga
    21061 cataccaatc tgtcgtattc attttgtcat agtgggcata ttctccttta agatccgtgg
    21121 tttttcctcc tgcagggttc aaagagttaa aactgataaa tgtatctcct gaaaattgaa
    21181 cggggctacc gtcctcatat gtggctacta gctgcacatt catttgtgtt gaatggtaaa
    21241 caattcctga gaaaagattg tctgagatcc aaagtactgt tcttttttta gatttactca
    21301 ttgcaccatt cgtattttta tagccatcgt ctcgaaaatg agtttttgta tagttgttat
    21361 ctagtagatt ccacggcaac gtatccgagt ataagtgaat attagataga actagcttga
    21421 tattgacttt tttcccgtta tacgtaccga tttttccata gtcaatattg actttggtgt
    21481 tggttgttac gccttctaaa acaaggtttg tttcaccgta ttctttttgg aaagaaggat
    21541 agcgtatatt tttgtttccc tccactaatc ctacgtagtc gtctgtaaaa ccacctttgt
    21601 tggcatcaac atgatatttg taaacttttg acgctccgtt tacagtaata tttgtttttg
    21661 cattaggaac atcttttgga ataataaaat tattctttac gttctggatt ggatttgacg
    21721 gatcgacaat aactttttca gcaaagacat tggctgtttt taacccaaac aatgtaccta
    21781 acatcatcat tatgggaaaa acatatttta accgtcttgt ccacgagtgt ttaaaattca
    21841 tcccttcatc ccctcttttt tataaatgac tagtgaaatt ccgacaataa cgatcaaatt
    21901 gatcgtagta aataatggat tcctaatgag cttttccata atatctagcg gcatggaagc
    21961 agctggtatg tttaaaatcc tcattaggta tagttggtag cttggcaaaa tagatagttt
    22021 agaaaataat aaaccgcagt acaaaaagac tgcggttaaa caagaaaaat tgaataataa
    22081 taatttgatc agtttcattt tatttcttcc ttaatctcta gaaaacaagt gagtaacaaa
    22141 atcccaacaa ttctcaaaga agtttttctt tacgccagta gaaacgattt tttttacttc
    22201 ttctttaggt tgcgaagctt ctgatttagg atagatccac acatccttta agaattcacc
    22261 gtttttatca gaaacaggtg ttaatagcaa aatatcctcg ctattagaaa tgttttcagg
    22321 acttgattca ttgacaatat aataagcttg tttctgattt gcctgtactt ctatcgagaa
    22381 ttcaaagaca cctgtttgtt gatcaattgt ctttgttttt ccattagaaa ctagcttcaa
    22441 ttgatcactg aatagttttt tcactcgatc ttttaattgg gattcaattt ggtcagtagt
    22501 gagatcctta acatccatct tttgtaaaat gtccgataca tcataaacag aaaacttcgc
    22561 gccatttact ccttcttgtt tttcaagttt ttgcccctgg ttgtctaatt gttcggaaga
    22621 aataattttt tgaacgtgaa tggtctgttt gactatttga tcgtcttcag caattttcgg
    22681 ttcttctgaa gaatcaactg ttgacgaatc gacggtttca tcgctgcttt gaatcgtgct
    22741 gctatctgac gtttggattg tttgttctgt gctatccgtg ctttcttgtc tgtctgcaaa
    22801 cgtcaactgt gtgtaggcag tgaaagaaaa aagagcggct gcacaaacaa atagccattt
    22861 tctatttctc atgttggtta ccctccttta ttttttcttt ttataaacaa atagcgaacc
    22921 aatgatcatg actgaaagaa aaacagtact tcctaaaagc catgtccaag ataatttttg
    22981 tttcttattt tcttcatctt ttaattctga ttttgtaaag gttactcgct tcccatacac
    23041 tagaaaacgg tgtgtgttga tccccttagg cgtacatgtc attagcgtta ctcgatcttc
    23101 attgggattt ggttctaagt aagtgacaaa gtttctttgt agttcgtctt tttgcacttc
    23161 ctcaatccga tcgatttgat aagcgtgctg ctcaccatca actttgatat aaaacttttc
    23221 tccctttttt actgaaggta aatcatcaaa aagattatct ttataaagtc cactatgacc
    23281 tgcgattaaa ggatttttac cgtttcctcc tgtaatatca ccagttcctt ccgtaatacc
    23341 tacaccattt tctaaaattt tgtcggacgt tccgtcataa attggtagtt ttaaagaaat
    23401 acttggaatt gtaagaatac caacaggatc gcctaactca atcttagcaa catccaaaga
    23461 agaatccgtt tctgctgccg ttcctgttcg atcgattgcg acaatctgct gattttgttg
    23521 gtatttcgcc acgctcgctt cagcaggcat caaaagtaag acacctacag ctgaaagtaa
    23581 ccagaaagca atgatccatt tacccatgat tcgctttttc ttaggcttcc tgataatcat
    23641 tggttttgat ccttctaggc tttcttctta aagtaaacaa ctgcagaaag tgcccctgct
    23701 cctagcaaca agcaagagat taatacatac gtttgaaaac cgttgctacc ggtcattggt
    23761 acggaagctt tttgattgtt tttgatcgtg attcggtttt cttctttgta tgaatctttg
    23821 tctactttga aagagatctc tttcgttaat ttttgatagt tttcaggcgc ttttgtttct
    23881 tccagtgaat attcaccttc agttaaacca gtgatttcaa agtgaccttc tttatctgat
    23941 tttaggatcg ctgcatttgc tacatcgtct acccatactg cttctttgac accgttttta
    24001 tccgtatcaa ctaatccacc ataatatttt ccttctgcgt ttttagcagc aaattctgcg
    24061 ccttcaagcg gtgttgcttt gattcctatt ccaaatagtc cgctatcgac ttttaagaat
    24121 tttgcaccat aagtcacaac gtcagctttc gttttttcgt ggtgttcttc ctgattaaat
    24181 ggattgttgt tataaacgaa atccacgcta tttgttaatg gattggcaac agctgcgcta
    24241 tcatttacat acatgttata agtcatttga agatcttgtc cgtgtaaact tgctacagta
    24301 tcacttactt ggccattatt cacgataaaa tcgatcacaa agccattttc tgaattttgg
    24361 attttataat ctgtattttc ttttaatacc acagatccat ctttatttgt tactttgata
    24421 tttgctttag cagcgacata agaaagtcct tgtcctgcta tatcccccca tttaaataca
    24481 ctgtaaggag aagtttcgct tttcactccg tttactgtga taccgcctgc tccgcctgca
    24541 atgtctgttg ggatatgaat cgttccttga taatttactg cgtcaccaat ttggaaagaa
    24601 tgttcttgtc ctgttccatt tgttactgtt ttatcgatca ctggtgcttt atagtttacg
    24661 tatgaagctt ttaagtcact tgtcacgcca tcattcgtaa tttcaaaggt taatttattg
    24721 tgcgcatcgt tgcgagcgtc tgcgcctaat aaatattgat ctgctgttgg ttctttatct
    24781 gatcccacta caacttctga aggaacttcc acaaaatagt attttcctaa cgtcaaatca
    24841 gtagcagtca gctttccttg atcatccgat acaagatctc ctaattttgt tccttttcca
    24901 ggttcgccct tgtataactc aaattttgcc ccttttaaag gtgtttgttt gctttgagct
    24961 gtgccatcgt caccaaattt cgttaattca aaactcaact tagaaacaat atttttagga
    25021 tataagtgaa tgtcttttaa aaaacctgaa cctgtattat cagtcattgg cgcaatcaca
    25081 accattggtt ttgctttttg tgtgactaat ccagcagcgg atttactttc ataaaccaca
    25141 tatacattcc ctttggaatt gacataggct ggttgatcag caaaagtaat ctcaccattt
    25201 tcatctacag ctgaagtaat tttattggtt gcattcttga cgtactcact gttagatccc
    25261 ttgtcttcta catctttaac gatttcgtcc actttcgcgt tggttagatc agaatcttca
    25321 gtcggtaata cttgatccgt aatatttcct aacgtaaact caacatcacc ataatctgcc
    25381 ttgttgtatt tcgttacacc tgtaggatat tgatcgtgcg tataaccatc gtttttgatc
    25441 ccgtcaatat caacattcaa ttgcgttcct tgatcgtaca ttaatttatg aaccgtgata
    25501 ttgaccttct ctggtggcgt tttttgtgtg acatcttctg catatgcttg atacccctga
    25561 aagcagcttg caatgattcc taacattcca acaagcattc cagctttttt tacataccga
    25621 tttattgcca tttttttatc tcctcatttc atatttcaac gactcgcgtt gattaaaacc
    25681 ttgttattgt agctctccct gtaccacgta tctttctttt ccttgtgacg tataaacaca
    25741 agtaaacaac gttaataagg gtttctctcc tttctttggc aaagacaaga aagagttctc
    25801 ttcaggaacg ccatcgatta atttaaagta ctcaggtacg ataaatttac gcgttacctt
    25861 gtaacgatag acgccatcgt tactaactaa gtccatcacc tcccctaatg acaacttttg
    25921 aatatcagaa aataaaacac ctggcatttc catgttatga cctgctagga catagtttcc
    25981 tttgcccatt tcgccatctt cataaaaata agtagccgct ccaagactca aagtgtattg
    26041 atttgtccct ttaaaaattg gcaatgctaa accaagtgca ggaatctgaa tttgtgcaaa
    26101 tacttgttta ttgacgccca tttcgattgc ttctttttgt atggacaaca tttgacccaa
    26161 agacatatca ttattgacgt tcgttgcatc ctccttataa ctctttgcgt gtgcattgat
    26221 ttgatctacg ggtatctttt tttctatatt ttgcttaaac acctctcctt catgtttttg
    26281 gtagctaaca tagccaaaca cactgcaaaa gacgattaaa gcgactccta aaaaaaaaat
    26341 aaccattttt tcatccatct gcctcctttc agataaaaca aaaaacactt tttcacaacg
    26401 aaaaagtgtt ttctcttatt actattctat ttcatttatc tggtggtttt atattggtgt
    26461 gtagccattg ctaacattaa gaatacaatt ggcacccaac tgattgtagt taatgtttct
    26521 tccatgaatg attgacacat gatcgctaaa aaagcccaca agaatgttag ttgtccgttt
    26581 gtcagctcac attttcttac gagcaaccat aaaaaactaa gcaaaggaat aatcgataga
    26641 gcaaaaccaa tcaatccata gtgattgacg agtgcgtgat acgtattatg gttacctaga
    26701 atctgatttc ctatatggaa aaagaaaggt ttcatcccta caaatatttg tcctgttttt
    26761 tcgcctaatg tttcatagaa tttgcgccaa atttcttctc gtcctgtaaa taaatgaact
    26821 ttgtctgaca ttgcagcaaa ataagaaatc ggtaaagcta gaaccgaaac tgctgcatac
    26881 atccaaaagg acaagtgtgt gtttcgaaaa atcttcttag gtaaaatttc taggataacg
    26941 aagataacaa gcgcgtataa tgctgctttc gattggcaaa cccaaatcgt tgccccagca
    27001 agaatcaacg ctggtaatga tagcaatcgc atgtaccatt ttacaaatga actagcaaat
    27061 cctgtgataa gaagggctaa tgtcattaaa gaactaccta tcgtatttgt attgatccaa
    27121 atatctgata atttgttcga tggattccaa atactttgtc cttctggtac tggtatctcg
    27181 gtaaatactc gataaaccgt tacgagtaaa aagacataga atactacaag atacaagcta
    27241 cgatcaaaca cagtaaattc cgtccatttt acaatcaaaa taatggcaat aattgtcgga
    27301 acgatcagat caccatagaa agtatctact cggtagcttc taaaaaaaga aaagaaaagg
    27361 taaaaaagaa tgcctgctgc aatgaagaat aaatcgtttc tctttacctt gtacagattc
    27421 aaaagaaaca aacagcctac gaaaaacgtc actaactgtg cagcattatc ataaataata
    27481 tcgtaggagg atttaacccc accgtccata ttaaatatag aatggatacc aacagtaaca
    27541 gaaaaatggc actcatttta ttgatcaatt tttctacaag ttcttgaatg tgactcactt
    27601 cccttattta ttgatttttt ctacattgac taattcgcac tggatcacat cgcacttttt
    27661 catattattt gcttcaaagt ctaacagtgt gagtagtggt tttttacctg cttttggcaa
    27721 ggataaaaag gaattgtttt ccttagattg aactacctgg ctgtaatcat aaatttttcc
    27781 tcttttggag acttcatact gatacttgta atgagcatcg attagatcaa tttcttctcc
    27841 ttcagataaa tcaatcatat cggataataa aacgcttggt gtatctacgt tataaccagc
    27901 aagaatataa tttccatttc ccatttcagc atctggataa taagtagaag ctactcctaa
    27961 actaagcgta tcgctattcg ctccttgaaa aacagggaga ataaatcctg tagaagaaaa
    28021 aacaagtttt ccagcaattt cacctttgag atccttttct aatgcctgtt tttgattagt
    28081 aataagttgt ctcttagaaa tatccgttaa actggttaaa ttatcttgat aggttttatc
    28141 ctgttcctga atatggattg tttttgttac accatctact ttttcagcga ttttgtcctt
    28201 ttgatgacct tgataagcaa aataactaaa aaggagaaaa acaatgatta cgcccacgta
    28261 aggattaaat aaagttttat tgtttttatt catctatcta ccttctatat ttgaattaat
    28321 ttacctacat ctaaccaaga taaaccgcgt taaattttac tattcttttt attaaataac
    28381 aatgccacat cataattgat cattttcata tcataatcgt caagaaactt ctaataatag
    28441 ttggcaatta gatcttattt tcacataagt tcgtctcaac tgttcaaaat ttttcattga
    28501 tcccaataga caggctttct cctttcctgt tatctgcatg tcactgatat attgcatgac
    28561 agataacctt ttaaggtgtt tttgtttcat cgtttttagt aacttttctt ccatttcttt
    28621 cgacactttt ttctattttc gatttcttta caattcatga gttttcctac cttccttgtt
    28681 ttgataaaaa taaaaaagcc tatgatcaca tacgctctac acttcactct tgggttatct
    28741 ttttctttct tttaactact ggccttgctg gtttcaaatc cttgattcca ttatagattc
    28801 tccaataatc aaactctttt cgtaatcttt ctttttccat atctgttcct ttggataagg
    28861 ctttaaatac atctttaggt gaataactac gagctaacag ctgtacatac cttccttttg
    28921 tcaaagtatc catatccatc gcatctaaaa ctggtcgcgc catctaattt gcctcctaaa
    28981 taaattcttt actcgatgtc taatgataat actagctttt tattattcgt cgtaatttct
    29041 gcacattctt cttcattatt ttctgcgatt aacgcataat agcatggtga ttttacttcg
    29101 aaaaatttca tgatctattc ctccaatttt tattcttctt tatccgctac aaagtcaatg
    29161 atttcaacac tgacaatata atcactcaaa tatgaccagg tatcttctac agacaattcg
    29221 tctcctttgt cgttcgtaag cgtcatttta aaccactcat cgccttcatc atcttcaggt
    29281 agcaagccga atccaattgg attatcagtc aatgtttaat cgtataaatc atcctcgtct
    29341 aatgctaaaa catctaatcc tttacaattt ccgcctcttc taaacgtcac aaatcctttg
    29401 taatcccaac gttggaaaga aaccttaata gtatgtgtgc ccaatcgtat attcggatca
    29461 taatttttca tttgtcattc ctccattacg taaatttcat tttctctatg ttttatatca
    29521 attttttcac tttattcagg acttgttcat attttatcgt ttttcgttta cttaaaaata
    29581 cataaaaaat ttcgactcgt ttatggcgtc cgaaacgaga aaccctagta gacagcggta
    29641 aatgctcgat tgatccaata atcaacgtcc tcgcaagtat ctacatgtac tgcagcacac
    29701 gacacaattt cttgtgcgtc ctccccatta cgagaagacc tactccttat tccatgagtt
    29761 tgcattaaag tctagatccc ttagtagctg ctcatgatta atctagacgc tattctaact
    29821 tgtctaactt ctaaattccc tcttgtttac tctgcgctta ttatttacac aaattcattt
    29881 caccactatc caaacaagcg catttactcg attgaccaat tttttacgtt accaatcgtt
    29941 aaaacctacc ccgtcactcc ccgaagggaa ttgttgatag atcaagtcta ctccttattt
    30001 ttctagtgac tctaagttcc tttagaagtc tcgacaatat tttcaagcaa gcgaaaaacg
    30061 ataaaataca agttatgaga atgatcaatt cagctgatca aacgccccat cccttttgcg
    30121 gtacaacgca agactccatt ttttcttcga ccaatcagta caccgttttg gtcataaaca
    30181 tgtttgattg ttggatatag ctcttttaag gggataggat cttttaaaat tatttgcttt
    30241 tgtttcccaa tgtaataaat catttcctct gagactttgt ctttctggat tatatatttc
    30301 ttacactgca caacggtttt actttggcca gttgatagtt tcttcgtgat tttctctgta
    30361 gcggtacacc gtttaccgta gtgacaaata tctttgagac tcaaaattcc tgtatatgac
    30421 ataaaataaa ccacccaatt tctttaaata tttttaacaa gtttttcttg ttatttttca
    30481 aaaatagaga attgattttc gttcatgaca aacaaaaaag agggaacgca tccaaacatg
    30541 cgttccctct aatttcagcg ttcgtcatac ggcaaagagc cacacggaca tttttataac
    30601 attatgctta ggcatagatt catcgtaata tcacgtattt ttaaccttaa cacagccaaa
    30661 aaaagtggta tcacttttga atatctgtta tactaaatcc gttgcgggta ataccgtaac
    30721 actgaaaggt taacgcatta agggccaaat cttaatgccg tgtatgaacg ctgacgaaga
    30781 ttaccagcct tccgagttca taccgccttt cttttttttg tattttcaat tgtccaaatt
    30841 tatcgtccaa aagtgcagaa cgaaaatcaa ttcatcacag gcatagcaga caattaaatt
    30901 tatacaaaaa aatgtaagcg aataatattc gtattgcctg aaaagaagta atatcaagat
    30961 ttttagcttg tttaaataat gaaattatct ctcttcgcgt attattataa ctcaaaaaaa
    31021 ag
//
LOCUS       DAZUMM010000026        30696 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000026 DAZUMM010000000
VERSION     DAZUMM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.026"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(71..157)
                     /locus_tag="ACG8E6_001138"
                     /pseudo
     CDS             complement(71..157)
                     /locus_tag="ACG8E6_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(566..1474)
                     /locus_tag="ACG8E6_001139"
     CDS             complement(566..1474)
                     /locus_tag="ACG8E6_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFS5061572.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKG
                     ITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFES
                     ILLAYSVLLSRAQRRFEGTLRYSLGY"
     gene            complement(1568..2617)
                     /locus_tag="ACG8E6_001140"
     CDS             complement(1568..2617)
                     /locus_tag="ACG8E6_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5061573.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFTRGAS"
     gene            complement(2699..3388)
                     /locus_tag="ACG8E6_001141"
     CDS             complement(2699..3388)
                     /locus_tag="ACG8E6_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS5061574.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(3494..3862)
                     /gene="rplL"
                     /locus_tag="ACG8E6_001142"
     CDS             complement(3494..3862)
                     /gene="rplL"
                     /locus_tag="ACG8E6_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFS5061575.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(3915..4418)
                     /gene="rplJ"
                     /locus_tag="ACG8E6_001143"
     CDS             complement(3915..4418)
                     /gene="rplJ"
                     /locus_tag="ACG8E6_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFS5061576.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(4622..4749)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(4828..5517)
                     /gene="rplA"
                     /locus_tag="ACG8E6_001144"
     CDS             complement(4828..5517)
                     /gene="rplA"
                     /locus_tag="ACG8E6_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFS5061577.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(5621..6043)
                     /gene="rplK"
                     /locus_tag="ACG8E6_001145"
     CDS             complement(5621..6043)
                     /gene="rplK"
                     /locus_tag="ACG8E6_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFS5061578.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            6386..7054
                     /gene="sdaAB"
                     /locus_tag="ACG8E6_001146"
     CDS             6386..7054
                     /gene="sdaAB"
                     /locus_tag="ACG8E6_001146"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFS5061579.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            7067..7939
                     /gene="sdaAA"
                     /locus_tag="ACG8E6_001147"
     CDS             7067..7939
                     /gene="sdaAA"
                     /locus_tag="ACG8E6_001147"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFS5061580.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(8030..9130)
                     /locus_tag="ACG8E6_001148"
                     /pseudo
     CDS             complement(8030..9130)
                     /locus_tag="ACG8E6_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(9222..10109)
                     /locus_tag="ACG8E6_001149"
     CDS             complement(9222..10109)
                     /locus_tag="ACG8E6_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5061581.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(10186..10731)
                     /gene="nusG"
                     /locus_tag="ACG8E6_001150"
     CDS             complement(10186..10731)
                     /gene="nusG"
                     /locus_tag="ACG8E6_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFS5061582.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(10832..11002)
                     /gene="secE"
                     /locus_tag="ACG8E6_001151"
     CDS             complement(10832..11002)
                     /gene="secE"
                     /locus_tag="ACG8E6_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFS5061583.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(11026..11178)
                     /gene="rpmG"
                     /locus_tag="ACG8E6_001152"
     CDS             complement(11026..11178)
                     /gene="rpmG"
                     /locus_tag="ACG8E6_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS5061584.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            11340..11984
                     /gene="cbpA"
                     /locus_tag="ACG8E6_001153"
     CDS             11340..11984
                     /gene="cbpA"
                     /locus_tag="ACG8E6_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFS5061585.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(12040..12543)
                     /locus_tag="ACG8E6_001154"
     CDS             complement(12040..12543)
                     /locus_tag="ACG8E6_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS5061586.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(12556..12602)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            13140..14105
                     /locus_tag="ACG8E6_001155"
     CDS             13140..14105
                     /locus_tag="ACG8E6_001155"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5061587.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(14204..15376)
                     /locus_tag="ACG8E6_001156"
     CDS             complement(14204..15376)
                     /locus_tag="ACG8E6_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5061588.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(15363..16508)
                     /locus_tag="ACG8E6_001157"
     CDS             complement(15363..16508)
                     /locus_tag="ACG8E6_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5061589.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(16522..17454)
                     /locus_tag="ACG8E6_001158"
     CDS             complement(16522..17454)
                     /locus_tag="ACG8E6_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5061590.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            17571..18650
                     /locus_tag="ACG8E6_001159"
     CDS             17571..18650
                     /locus_tag="ACG8E6_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS5061591.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            18647..19276
                     /locus_tag="ACG8E6_001160"
     CDS             18647..19276
                     /locus_tag="ACG8E6_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5061592.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(19386..19613)
                     /locus_tag="ACG8E6_001161"
     CDS             complement(19386..19613)
                     /locus_tag="ACG8E6_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5061593.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(19716..22304)
                     /locus_tag="ACG8E6_001162"
     CDS             complement(19716..22304)
                     /locus_tag="ACG8E6_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFS5061594.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(22324..22983)
                     /locus_tag="ACG8E6_001163"
     CDS             complement(22324..22983)
                     /locus_tag="ACG8E6_001163"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5061595.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(23032..23535)
                     /gene="trmL"
                     /locus_tag="ACG8E6_001164"
     CDS             complement(23032..23535)
                     /gene="trmL"
                     /locus_tag="ACG8E6_001164"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFS5061596.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(23756..24622)
                     /locus_tag="ACG8E6_001165"
     CDS             complement(23756..24622)
                     /locus_tag="ACG8E6_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS5061597.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            24731..25372
                     /locus_tag="ACG8E6_001166"
     CDS             24731..25372
                     /locus_tag="ACG8E6_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFS5061598.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(25434..26795)
                     /gene="mgtE"
                     /locus_tag="ACG8E6_001167"
     CDS             complement(25434..26795)
                     /gene="mgtE"
                     /locus_tag="ACG8E6_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFS5061599.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(26813..27712)
                     /locus_tag="ACG8E6_001168"
     CDS             complement(26813..27712)
                     /locus_tag="ACG8E6_001168"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS5061600.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(27714..28511)
                     /locus_tag="ACG8E6_001169"
     CDS             complement(27714..28511)
                     /locus_tag="ACG8E6_001169"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFS5061601.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(28489..29193)
                     /locus_tag="ACG8E6_001170"
     CDS             complement(28489..29193)
                     /locus_tag="ACG8E6_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFS5061602.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            29324..29899
                     /locus_tag="ACG8E6_001171"
     CDS             29324..29899
                     /locus_tag="ACG8E6_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFS5061603.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            30017..30694
                     /locus_tag="ACG8E6_001172"
     CDS             30017..30694
                     /locus_tag="ACG8E6_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFS5061604.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         9081 a         6437 c         5470 g         9708 t
ORIGIN      
        1 atgaaatacc tctttgttac tcataattat accataatta gtcacgaatg caaccaatag
       61 aaaaaaatac ttagaacggt agatcatcgc taataacaat gcattgtcca ccaaatggat
      121 tttgtgaagc attaaataca ttgttattaa ttgtcgctgc ttttaactag gcctctcccc
      181 ttctattata aaagaagata tatattattt cttaattacc tactacctta aaccaaaatt
      241 taagtactac ctaatttaag tagtacttaa ataatctaaa aaatagttat cccaattgtt
      301 atgcttttat aatacgatta tttgtaacaa aatgaatatt atataattaa acactaccat
      361 atttaggtag tgtttaatta ttagcaaaac aaaaaatatt tattctattc tcttaacttc
      421 aactaagtta tgctgcacac ctttatataa atcatatgca actcattagc tagacaaact
      481 acaagcatgt agttttatct gttaattatg tactacctaa ataaattagt ttataattaa
      541 caagtacccg tggtgctagt taattttaat atcctaaaga atatctcaaa gttccttcaa
      601 aacgtcgttg tgctcgactt aatagaacac tgtaagccag taatatgctt tcaaatctac
      661 tctctagccc tttaacagaa cgtgagttga aattttgaca acctaatttt tctaaggaag
      721 aaaaaacagt ctcaactgtt tttctttgtt ttccaagtaa gctgtggtct agtttttctg
      781 atttatccat gtttttacgt ggcggaacgg atatagtaat gcccttaagg gcaagctttt
      841 catggatttt atggctaata tatcctttgt cccctaggat attgggaaga ttcgcttctt
      901 ccgataaagt ctctaacact ttcacgtcat ggacacctgg attcgtgatt gagtaagtaa
      961 tcggaaagcc agtcttagta acgatcatat gaattttaag accataaaaa taagattttt
     1021 ttgttgagtt atagccaact ttggctattt gattcaatag ttttgcttgt ctatttctaa
     1081 ctggtttaca taatggacta ggaaaactat ctataattcc gactagttca cctttggtga
     1141 gcttcttgat gaaaaagtat cgaataatct tgatagtgta agctaagttt gaacttaagc
     1201 gagtgaatcg actcctacta ggaaagtcac cattaggaaa taagacggaa caaaccgctc
     1261 tatacgtggc tctttggcta gtatagccat taattatgcc ccatataaca caagcaataa
     1321 tcactgtatc gcgttgcttc agttgatcag tatttcttcg gtttttaatc ttatctggta
     1381 cacaattgtg gtatatgtta gaaacagttc tcataatttc cttgaatgtt ataaaagtat
     1441 ctgtataatg ttcagtatat tttgatgaac gcatatgtag tcctcatttc ttgggagttt
     1501 tgagactact atatgcgttt ttttgttgat ttaccaacat gtttagacat tttcctctag
     1561 gaaaaaatta actagcacca cgggtaaata cagtattatt tcggttgctt gctgcttcac
     1621 ctagcccttg tactcgtgtt tgattggcga cttgaccaaa ttgattcccg acgtctaata
     1681 aaataatagc taaaagcaaa atggccaaat ggcttccgcc aaccaataaa gcaataaagc
     1741 ttattgtttg cataacaagt cccatataca cgatttttct ttcagaataa gaatctgcca
     1801 gtcgtcccac gataggtgca gcaactgctc cagacaaacc aaaaatcgct aatatccctg
     1861 cttcgaatgt tccccaattg taggcgggac tgctgatata aaaaatcaac gtagaccaaa
     1921 agatcgaaaa tgttccaaac atgaaaaatc cactgactgc cgcttctctt aatagacgtt
     1981 gagaagcaac taaatgcggc aaacttttaa gtgaatcaag gtatgaaaca gctgtactac
     2041 tttgctggac agaacgcggc attttccagt aaagcagaag tgtaagaatt ccgactgcac
     2101 ataaagcaaa taaatagatc atccgccaag aaaaaagact ggcaacaata ccagataccg
     2161 tccgagaaag caaaatcccc acgagtaaac cgctaagcaa tgtacccatg accttccctc
     2221 ttgcttttgg tcccgctagt accgcaccgt aaggaatgat gatttgcggc acgatcgaaa
     2281 gtaatcctat taaaaaagaa gcgcatgcaa ataacgtaaa cgaaggcgca aaaaaagcgg
     2341 ccaacagaga gacggcagat aacgctacca ttcgaatgat caattttgga cgatccacca
     2401 cgtcccctaa aggaactaaa aacagtaacc ctaacgcata gcctaattgg gtcaacatcg
     2461 tcaatatccc gactgcactt gtgctaacag ataaactagc tgctactttt gttccaatcg
     2521 gctggatgta atacatattg gcaacaacaa cgccacaagt gattgataat agtaaaataa
     2581 gcgaagttgt taaaggtgtt ttttcattac tcttcattga tttcccccat ttcctatctt
     2641 agtttacaac ttatggaaaa gattggaaat agaaaaatac aactattcgc cacacttatt
     2701 atttgtgctt ttgttttcct tgttttttct tgcgatcttt ataaatgaag acaactaacc
     2761 ctactagcac agtaacactt gcaaccaacg tgactgcagt tttgttatat ccgtgaaaca
     2821 gagcttctcc accacacgca tacaacacag acgaagggat gactccaatc acgatcgaca
     2881 atatgatttg ctgtagaggc aatttcatcg catctgcagc aaaattgacc atagaagatg
     2941 gaataaccgg tatcatatag cctaacatta cacctatttt tggatgtttc atttgtcgga
     3001 ttgcttgcac ccaatgattc gtttcggttt tcttatctaa aaattttaag cgatgcataa
     3061 ggaaaattgc agtgatgtta ccaagagcat tacccaaaat gttgatgatc gcacctgtca
     3121 gtggtccaaa gctcaaacca gcaatgactc caactactga agtaggaata cctggaacag
     3181 cacacatcac cgctgtcaat acgatcaaga gtatcgatgc aaaagtccca tgcgaacgaa
     3241 tatgtgcgag tagctctgcc ttactcgctc tcggatttaa aaataattgg atgtctgtct
     3301 gatattctaa atacaaccga tagcccacta ccagtatcag aatgattccc aacgagatca
     3361 acatcaatcg gattattttc tttttcatat ttcctcctga aaaaaatcta tattcattaa
     3421 ataatagttt aacataaaaa caaaaaagca ccgaaatcca acataggatc tcagtgcttt
     3481 taaatagtaa aaattattta acagttacag atgcgccaac ttcttcaagt ttagctttca
     3541 attcttcagc ttcttcttta gatacgcctt ctttaactgg tgcaggagcg ccatcaacta
     3601 cagctttagc ttctttcaag cctaaaccag ttgcttcacg aactgctttg ataactttaa
     3661 ctttttggtc tccagctgca gttaattcta cagtgaattc tgtttgttct tcagcagcag
     3721 cagcgccacc agcagcagct acagctacag gagcagcagc agatacgcca aattcttctt
     3781 caatagcttt aactaggtcg ttaagttcta ggatagttgc ttcttttaac tcagcaacga
     3841 tgttttcaat gttcaatgcc attataattt cctccgttta attgttcttt tttatttaat
     3901 gctttatgcg gcaattaagc tgcatctcca tcttctttgt ctgccactgc tttgacagcg
     3961 taagccacgt tgcggactgg cgcttgtagt acagatagca acatagatag aagtccttcg
     4021 cgatttggca attttgccaa tgctgtgatt tcttccaatg aagagacttt accttcgatg
     4081 ataccgcctt tgatttctaa tgcttttgct tctttagcaa attcgtccat gattttcgct
     4141 ggtgcgacta catcttcgtt actgaatgca acagctgtag gtccagtgaa tacttcgtcc
     4201 atgccttcaa ggccagcttt tttagctgca cgtgaaagaa tagaattttt gataactttc
     4261 atttctacgt tagcatcacg aagttgttta cgtaaacgtg ttacttcatc aactgttaat
     4321 ccgcggtagt caacgactac tactgatgca gctgctgtaa atttttcgct tacttcatca
     4381 acgattgctg ctttttttgc gataattgct tcactcattt ttgagtttca cctcctgaat
     4441 tttgatggaa gagttttttg cactgaaaag gcaagtcact tttatagtta ctttgctcaa
     4501 ccagtattat tcatcaccta ttcacttcgt tcatagtgat tctactcgct ttgctcgaaa
     4561 ggaaactcct ttcgctttgc tcaaccagta ttgttcatca tctcttcact tcgttcatag
     4621 tgattcacaa taaaaaactc tatgccaccg cagacataga gggactattg aatatattca
     4681 ataaacgtcc tcggtaggaa attaaggctt gatgccacct actgtcttcg gtacaagtta
     4741 attattaacc tcaactagct tagcatgcta aactagttga gtcaagaatt atttttcagt
     4801 tggatcaatc aacattctaa tatttaatta gaaagaagct tggtcaacat ggataccagg
     4861 tccaaatgtt gttgtgatcg ttacattttt catgtattga cctttagctg ctgatggttt
     4921 agcttttagc aatacatcat ggattgctgc aaagttttca actaattttt catcgtcaaa
     4981 tgaaactttt ccgattggta catggatatt tccagctttg tccacacggt aagttacttt
     5041 accagctttc acttcgttta ctgcttttgt tacgtccatt gtaactgtac cagttttagg
     5101 gtttggcatt aagccttttg gtcctaatac tcgtcctaga cgacctacag tagccatcat
     5161 atcaggtgtt gcaacaacta catcaaagtc aaaccatccg ttttggattt tttgaaccat
     5221 gtcgtcgtca cctacgaaat ctgcaccagc tgcttcagct tcttttgctt tgtctccttt
     5281 agcaaaaact aaaacagttt gtgttttacc agttccgttt ggtaatacaa cagcaccacg
     5341 gatttgttgg tctgcttttt tagggtctac gttcaaacgg tatgcaactt caactgttgc
     5401 atcaaatttt gcaatgtttg tttcttttgc taaagctact gcttcggcaa caggataaac
     5461 tttacttgaa tcaacttttt ttaatgcatc ttgcattttt ttgctctttt tagccatttt
     5521 ggttcctcct tgattgtggt tataacggtt gtacctccca cgtgttctat atctttcaat
     5581 atagagccaa ctgagaagct gctgtcttag ggtgtataga ttattctaca gtgatcccca
     5641 tgcttcgtgc agttccttca accatgcgca ttgctgcttc aacgttagct gcgtttaggt
     5701 cttccatttt taattcagcg atttctctta cttgatcgct agaaactttc gcaactttat
     5761 ttttgtttgg ttcgcctgaa cctttttcga ttttagctgc ttttttcaat agaacagcag
     5821 ctggcggtgt ttttgtaacg aatgtgaatg aacggtcttc gtatacagaa ataacaaccg
     5881 gaatgatcaa acctgcttga tcagctgtac gagcgttgaa ttcttttgtg aatcccatga
     5941 tattgatacc cgcttgacct agtgcaggac ctactggtgg agctggagtt gctttacctg
     6001 caggaatttg caatttaacg attttttcta ctttctttgc cacgagacat acctccttaa
     6061 gtccgtgatg tggttaattg gagatgcata tttctcctcc cacgttttat ctgatctgaa
     6121 aaaatccaga caaataatgt gcaaaacaat gcacactggt acagtatagc aaatttattt
     6181 gttttttcaa gatttttttc tgctattttt acattttgct tgaataaacg ctaaatttat
     6241 tctaaaaaaa caacaatttt tcgttctcat ttcttctttt atgttacact tgcgctagca
     6301 tggttcaaca gatgaaagca aacaataaca cggtttttta gacccttgta caaagattcc
     6361 tacataacaa aatgaggtgg agaaaatgaa agatttaagg tatagaagtg tctttgatat
     6421 tattggacct gtgatgattg gtccaagtag ctctcacact gccggagctg ctagaatcgg
     6481 gaaaatcata cgtagtatat ttggcgaaca gcctgattct gtagatattt atctatatga
     6541 atcatttgca aaaacctatc gggggcacgg aacagatatt gcattagttg gcggcctgtt
     6601 agggatggaa cccgatgatg aacgtttagc tgattctttg aaattggcat atgaagccgg
     6661 tatggaagtc ttgtttgttc ctaagagtga aaaagccgaa catccaaact ctgtaaagat
     6721 gcttctaaaa aaaggaacac gaaagctttc agtcactggc atttcgatcg gcggtggcaa
     6781 tatccagatt tctgaattga atggattcaa aatttcttta agcatgggta ctcctacctt
     6841 gatcgttgtc caccaagatg ttccaggtat gattgctaaa gtgactagta tactttctga
     6901 gaccaatacg aatatcggca ctatgacagt gacacgggaa tcaaaaggtg aaaatgccat
     6961 tatgataatt gaagtagatg atcctcaagt agaagaaacg gtaaaaagac tcaaacagct
     7021 gccaaatatc gacagcgtaa attatttcga ttaaggagct aaaaaaatgt ttttatcgat
     7081 tgaagaatta gtagatcaag caaaagctta tccaagtgta gcagaattga tgattgcagt
     7141 tgaaatggaa atgagtggcc gttcccgtag tcacatcatc gagatcatgg aaaaaaacct
     7201 ctcagtaatg gaacaatcaa tcaacgaggg aaaagaaggt gttacctccg tcacaggaat
     7261 taccggtggt gatgcaaaaa aattagatga ttatttaaat gaggcagact ttttaagtgg
     7321 agaaacaatc ttaaaagctg tacgcaatgc tgtagctgtt aatgaagtca atgcaaaaat
     7381 ggggttgatt tgtgctacac cgactgccgg aagcgcgggt gttgttcctg gtgtgctgat
     7441 ggctgtccga gatcggcttg atttgacaca tgaacagcaa ttggaattct tatttaccgc
     7501 tggcgctttc gggttagtca tcgcaaacaa cgcttctatc agtggtgcag aagggggatg
     7561 tcaagcagaa gttggttctg caagtgcaat ggcagcagca gctttggttt gtgcaaaaaa
     7621 tgggactcct catcaagcag cacaagcagt agccattaca ttgaaaaata tgatgggact
     7681 gatttgcgat cccgttgcag gcttagttga agtcccgtgt gtcaaacgta atgctttagg
     7741 ctcttctcaa gcttttatct ctgctgatat ggcattagcg ggtatcgaaa gtgtcattcc
     7801 gccggatgaa gtcgttgctg caatgtacca agtcggacgt caaatgccgt cgattttcaa
     7861 agaaacagca gaaggtggct tagctgttac gccaactgct aaacgacttg cggaagagat
     7921 tttaaataag agggcgtgat aaagctcctt aatttcgagt attaagagat tagttatctt
     7981 cataacaaaa aacgaagaga ggactaattg cccttctctt cgttttttat taatccatca
     8041 atttcgtatt ttttacttca tcgatcgttt ttgttcctgc aagttgcatc gtgatcgaca
     8101 attctttgtt caggtgttca aaaacagatg ttactccttc tgccccacct agattcaacc
     8161 cataaatcac tggtcggcca atcgcaacta agtcagcacc acttgccaga gctttgaaga
     8221 catgctctcc tcgacggaca ccagagtcga aaatcactgg tacacgtttg ttgactactt
     8281 cggcaatttt tggcaatact tcaaaggacg caggacctcc atctaattga cgtccaccat
     8341 gattggatac ccagatacca tctgcccctg cagagatagc gattactgca tcttctggcg
     8401 attgtattcc ttttacgata actggaagat gggtgatttc tttgattgtt ttgatgtcat
     8461 ctggtgtcaa gccttgtttt gcagctgcat aaatttctgc gattcctttt ccttcgccat
     8521 taccacttgc actttgttcg ctatacgcag ctaagttagg cattggtaat ggaaattgga
     8581 attgattgat gacgtcttct tcacggtaac caccgagcgt tgaatctgcc gttaaaatga
     8641 tagctttagc ccctgcttct actgctttgt tcaaaatgaa ctcattgaat ctgtcgtctt
     8701 tactcatata taattggaaa aactgcggtg cgcctggcgc tgcatcggct gcgtctttga
     8761 ttgtcgtatt tgcataagtg ctgatggaaa agattgatcc agcagcagct actcctttag
     8821 ctgtatcagc ttcccctttt tcatgtgcca atccttgggc cgctgatggc gcttggataa
     8881 tcggtgtatc taattcaatt ccgaaaacag atgtatgcaa atcggctgag tcgattccgc
     8941 gtaatatccg tggcatgatt ttctttgtat taaaagaagt cgtattttct ttcatcgtcc
     9001 actcatcttc agaaccaccg cgaatatagc caaaagctcc tgcttccatg cgctctttta
     9061 cacgaccttc tagttaggca atgtttacaa tatcgattgg atgttcttca ttacttgtat
     9121 gataagtcat agtagatcct tctttcatct tcttgattac atataaatca tactaaactt
     9181 acaaaaagta agcaaacatt tactctcaga tacttttatc gctattcttt ccaaataaat
     9241 cgacttacaa gctgatctac ttcttggttt tcatgaagca tactatgaga ggcattttca
     9301 cctgtcacta ctttttcctc tatttcattt ccgcgttgct tcaacaacgc atagaccgct
     9361 aatgcactgt ttaacggtac tgttccatca ctcaagtctg tttcatctag ttgaccagca
     9421 atcaagaaaa atcgagtggt tataggaata ttaccgatca tttgctggta atcttgatag
     9481 cggttagaaa cgacagcagg cccctttgct aactcatctg tcagagactg cgcactatca
     9541 tcgacaaaat cattaaaagg cgcgccaatt gctgttaatg tctgaacttg tggcaatgac
     9601 gtatcctgtc catacgttcc taaatagcgt agtgcactaa ctcctcccat ggaatgcccc
     9661 actatgttga ctttatctat gccgtagttt tctttcagat acaaaagaca agttttgatc
     9721 cattctgctt gattccactc gttattttta ttatcttcaa acaatacttg gaccatcgga
     9781 ttgtctgctt ttccagacaa atctcctgtt gcttgtattt gtccatctgt tcctactgtt
     9841 aaaaccaatt cttttactgc ttgctgattt ttttccaaac gccgcaacat ccctttaaag
     9901 gaattttctg tcccgctgta gccgtggaaa aaaagtgttg gaatagtggt tttttctatc
     9961 actttactgg attctgccgt tttttcttta tcagaaactt cagtagtatt tatcttcgtc
    10021 gtctggcaac cacttaacgc aaacaaactg ataaggacta aagctgctgc agttgcacgt
    10081 ttgcataaat ttttgattgg ttctgtcata cacctcttcc tttccatata gaaaaaagaa
    10141 agacttgaaa ggccaatcat atgatcgaca tttcaagtct ctttattaat cgattttatc
    10201 tacttgatcg aaatcaagtt ctgtactggt ttcacgtcca aacatatcga tgttgacttt
    10261 caatttttct ttttctccgt caacttctgt tacttgtcct tctaatccag caaatgcgcc
    10321 ttcgatgatt ttcacgactt cgccaacttc tacttctaga tcattttgtc gcaggctcat
    10381 accgatagaa cgcaagatat gattgacttc ttctggaagt aatggtgctg gtttgcttcc
    10441 ggctccgtgt gaaccaacga atcctgtcac accaggtgtg ttgcggacaa cataccatga
    10501 agcgtctgtc atgatcattt caacaagtac gtatcctggg aatgttttat gaacgatttc
    10561 tttttctttc ccatttttga cttcagtttc tttttcttca ggaacaacga cacggaaaat
    10621 aaagtcggcc attttcatac tttgcgcacg agattcgata tttgctttta ctttgttttc
    10681 atatcctgaa tatgtatgca agacatacca gtttttttca aaagattcca ttgaaggctc
    10741 ctcatttcat tgtaaaataa aaaaacctca gtacacccga agtttttctt tcgcttcatt
    10801 atagcactaa aagtacgttg tggctagcga gttattttaa aatccagcca aataagcttt
    10861 ggatggctgt atccattacg taaaacatca atccgaaaat aatagaagtt tcgatgacta
    10921 ctaacgtatc tttacggagt tgttttctgc ttggccaagt tacttgtttg atttcatctt
    10981 ttacgctttt tagaaatttc atgagacaac ctcccttgct actgtttatt tcgtttcttt
    11041 atgtaatgta tattgattac aatatttaca aaacttattg atttccaaac gttcgccgct
    11101 ttttccttca ctgactgatt ttgtgtagtt gcgagaccca caaacggaac aagcgagtgc
    11161 tgcttttttc tttgccattt acttctgccc ccttgctgag aatcagactt atacttcata
    11221 acagtatcac gtaacttttt acttgtcaat ttgtgaaata aactatctat tgttttggat
    11281 aaaattatga tatgattaat ttgaaattaa gctaaaatga gacaattgga ggtatagcga
    11341 tgctattaaa aacacttgta tataagaaaa aagatctgac aaccgttaca gagactagta
    11401 cattagaaga agcattaaaa attttagaaa cttccggata ccgttgtgtg ccgatcttag
    11461 attcttcagg taatattttc cgtggaaaca tttacaaaat gcatatctat cgtcataaag
    11521 caaatggcgg tgatatgacg cttcctgtca ctcatctttt gaaaaacgct acaaagttca
    11581 tttacctgga tacttctttc ttcaaagtat tctttacgat caaagagctt ccttatattg
    11641 ctgtccttga cagtgaaaat catttttatg gtattttgac acatagtacg ttgcttaata
    11701 tgctctctca atcttggagc gtagaacaag gaagctatgt actgacaatt gcttccacag
    11761 gaaagcaagg agatttggct gctattgcaa aaatcatttc aaaacacagc agcattgcca
    11821 gctgtattac tcttgatatc gaaaaagacg aatacattcg acgtacattg atcacgctac
    11881 cggctaacac tgaagcgaaa gtatgtgaaa cgatcgtcaa ccacttagag aaaaagaatt
    11941 ataaagtggt agaaattgaa aatttgcaaa accaagaagc ataatcaaaa agcgaaaaaa
    12001 gaaaacagct aatctgcttt cttttttcgc tttttataat tagacacgct tttcaaaatc
    12061 aagcgcgcga tctaaataat acatcaatgg tgctgaaaca gccatgacga taaactcact
    12121 taatgcaagc gtgccatagg ttgtccaaaa tgcttggcca cctgttggaa ctaacatata
    12181 agcaatcagt cccatgctga tggtaaagaa gatcgtgttc aatactaaac gcatctttac
    12241 atttttcact ttgttttgca ataaagctgt caggcctaaa gaaagcaatg tttggccgcc
    12301 accaaaaacc acatccatga taccaaaacc aaagaaggca ttatagacga tgaccccgcc
    12361 taaaactccc caaagcaatt tacggttaaa gacaactaaa tgattcaaac tttctgaaat
    12421 acgaaactgg atcggccctg aggatacagg cgcaactaaa atagttaacg ctagataaag
    12481 agccatgatg atgccattta caacgattac ggataaccgt tgtctgcttt tcgttttttg
    12541 cattttctct cctcttttct cctagttttt ttaacgcggg atggttgatg aaccgcggtt
    12601 atgtgaaaca aacaataatt ataacagact tttcatttat ttcaatccgt cttatctgct
    12661 tttaaaaaag caaaagctta aacaatgtaa acgtttgcta aaaatcgtgg gatatggtat
    12721 aaagtaagtg taaggggtca taagaattcc cagtaaagca gacgaagtag gatctgcaag
    12781 aacagaattg ttgtgaaact taaataaata aaaaaggatg tgggaatttg agcaacctct
    12841 tactagagaa cggtctagca agcagctgaa gatctaagtg gacatgccga accttggaac
    12901 ttctccttac gtaagtaatg gatcatgcgc ttgctaggct tttttgtgtt tttcggtctt
    12961 cggacctttt tcattttcct agacttctgc tataataacc aagaattaat tttgtaacag
    13021 atttttttga aaaaacaagt ttctttcatt caaaaaatag aaaaagcttg ctaattattg
    13081 gttaaccagc aaacaggagt attaaaatat cttgttttat tgaatagcag gaggaaatca
    13141 tgaaattcca ttatcatata ttgatcaatc catcggcagg cagcggaaat ggctataaag
    13201 tagcagaaag aattttaccg gtattaaaaa ataagcatat tgactataca cttcattata
    13261 gtgaatacaa aggacacgat gctgagattg ctgaaacttt ggcaaaagaa acattgattc
    13321 cttggataga agaagaacag aaaacaatcg atactttccc ccttttgatc atcgtaggag
    13381 gtgacggcac cttacatcag gtacttgata ctttctatca gatggaagta gaattcccag
    13441 ttgcttatat tccagcaggt tctggaaatg attttgctcg aggggcaggc cttccaaaaa
    13501 atcccaaaaa aggacttcag ctgattttag cagctcaatc accagaaaaa gtccacataa
    13561 tggcttatga agaaaaaata agtgaaaaaa aaggaatagc tgttaataat ttcggtatag
    13621 gattagatgc agctatcgtc catgcgacaa atcattcttc cacaaaaaag cgattgaaca
    13681 aatacaactt aggatcattt tcgtatttat tctctattct ccgtgcttta ttcactcaaa
    13741 aaggatttcc aattctggta gatgcaggag gaaaacaatt aagtttttca aacgcatttt
    13801 tatgtacagc aacgaagcat cctttttttg gcggaggaat cgcgattgtt ccaactgctg
    13861 atgcgtctaa accagtcatt gattttgtcg tggtcgaacg aatcaatttc tttaaaatct
    13921 tgtggctcat tcttttactt atacagaaaa aacaattgaa atcgaaatat ttccatcact
    13981 acacaacaaa cagattacgt atcgtttcaa caacaccgca acatggtcaa gaagatggag
    14041 aagaaatgga aaaacgacct tttgatatca caataaccac aactaaacag ttgttttggt
    14101 gttaataaaa gaggttttgt cagaagctac atctggaaca ccgtttattc ggaaaaaagg
    14161 actgtgacac gacttttgtc acagtccttt ttttactatg cagttaattc tttgcccaat
    14221 tgggttgcca tcgatagttt ctttctttac ccaaaataaa ttggatcatt gaaaacaccg
    14281 cagcaaaagc aactaagaca caaccattga accaaaaaga ttctgcaaat gtttgattgt
    14341 aactgagtgt ttcactgatc aagttattat tttgaacaaa ccaatatgtt ggtgtgaatg
    14401 ccgctatttt gctaaccatt tctggcaagt attctgctgg tacaaacacc ccaccgataa
    14461 agcaactgcc tgtgataaaa atatctttga tcccaccgct ggtttctgag ttttttacca
    14521 atgaagtgat acaagccgaa aagctaatga tcggcaataa aaacaagaaa gtatttaaaa
    14581 tatgatacca taccgttata tcccacgagg tgtggatcac taacagaacc attagaagta
    14641 agaagccact gaacaacagc agcgagtaac taatcaaact aaaggttact ttgcgattca
    14701 agtttcgttt ggtaatgggc gcactctggt ttcgtttact gatctctttg cgattaaacg
    14761 ctagattgat gacgctgaaa ccgctaaaaa tggatagaaa caacccataa gacaagaaat
    14821 taaaggttgt gcccgtcaac agctttttca tccgctgact atatcccgca cttagctcaa
    14881 cttctccagt aaccgagagt gccatttgtg tctgttccat agctgcatcg attgttttat
    14941 cttcttcttg aaacagaatc agtgtttgca aaaaggtgtt cacttgctgt gtaacaactg
    15001 ttttagaaaa ggcatctggg cgactctgta tcttgatcgt cgccatcttt cctacttcga
    15061 cttgttcggt aaagtctgca ggcagatgta agataatgtt tacacgatta aaataaagag
    15121 catcatcgat ttctttttgt gaggttccat ccaatgtaac gactgtttcc ttgtcattca
    15181 aatatttacg aaggccatcg actacaggcg actcttcttc actaatcaag gcgattttcg
    15241 catcttgcag agaggtattt tcaacatcaa tattttgcgc aaaaaagaat gtggcaacaa
    15301 cagtgataat tacacctatc agtaaattcg atttatttgc tttaagcaat gtccaaatcg
    15361 ttttataaag cgtcataacg cacccctctt tccatccaag catttcctaa cgcaaaaagg
    15421 aaaataaatc cgatcaacca tagaagattg gtataaaaac tagccaaatc agtgtagaaa
    15481 taaagtttat acaaactttc gctgatcaag ttgacgagat ttaattgacc aacaatcgga
    15541 atatgaagat caatccaata cttcacttgt tccgacccca tcataccagc aaaaaagctc
    15601 atcgcaatcg tcactgaaat caagatgcta tccttttgca ccagattcat ttttggtatc
    15661 aaattaccaa ccaatgtacc taacaaaatc gcacacaacg accccagagc ggtaacaatc
    15721 atcaaccatt gccaacgttc accaaagtct acttgataga taaaacgaaa gacagctaac
    15781 ataatataaa tctctgtcaa aacaatcgta aagcttgctg ccaaattagc gattgaaact
    15841 aacgattgct ttctaggaat caaggacaag cgaataccat ttgacgattg atttgcttgc
    15901 tgatcctgta cattgcggac cccccacatc gtcccgtaga gaatcgccat tccaatcaga
    15961 gtgaagaaat aaaaactttt gaaactcata ttacgacctg catgaacttc ttgaaaagat
    16021 acttcttgtt caaaaacagt catgatgggc gcgagatcaa cgttctccac ttctgccact
    16081 ttactcgaaa ttataccaat acgctgtaaa aattgatcca tgacgttttt catcaacgtc
    16141 tgactcattc ctgcttgacc cacatggagc gaaacagatg aaccatcaat gagataaaaa
    16201 ccagccaaat catctgcatt tagcttttca ttcgcttcta cttccgatag ttcctgtact
    16261 tcaaataatg gtttatcatt gacttcgact tgttctaaga ctgtggtaaa attttccgaa
    16321 agcgctgttt gatccgcttt gattacaatt ccagtattaa tcgtttcaag attgccacct
    16381 tgatcaatct cgccaaaggc cgccataaac atcaatccta aaagaattgg aaaggccaat
    16441 gtccaaaaca acatggattt atttcgcagc agtatttttg tgcgatattt ataaaaatgc
    16501 caaaacatta tttcccctcc tctagtctct caaatccttg ccagtaatct ctaaaaagac
    16561 atcattcaag gacgcttctt tggttgctat ttttccgtaa ctgatctttt cttcttgtaa
    16621 gattgtaaga atcggcaaca atactgtatt tagttcagta gttgttactt ggagctgacc
    16681 ttttttataa gcgattccat cagaagctat tgttcgctga atcgctgaca acacattttc
    16741 tgggagtaat gggatgtcga tcgtcaaacg atccgccgtc cgtaccatat cttttagaga
    16801 ttctttggtt cctttagcaa tcacctgtcc ttgatccatg atcacgatct ggtcgcaaag
    16861 aatttctact tcttccatgt aatgtgttgt atagacaatg gttgctcctt gtcgattcag
    16921 ctccttgata ctttctaaaa ttttattccg actttggggg tctacagcta ctgtcggctc
    16981 atctaaaaaa attaaccgcg gcttgtgggc aatcccacat gcgatgttca accgacgttt
    17041 caagccacca ctaagttctc ctggacgaaa cgaaacatat ttttctaatc ccactaattg
    17101 aatcacctct tcaatcaagg ctttcctttt ctctttttcg gaaacatata aaccgcaaaa
    17161 ataatcgata ttttcttgca cacttagttc gtcaaagacc gcaacgtctt gaggaacgac
    17221 cccaatcatt gccttgacag cataattttc aggagtcatt gttgtcccga acaatttaat
    17281 ggttcctttg tcataattta acaaagaaag catacagttg atggcagtcg atttcccact
    17341 gccgtttgga cctaacagac ctaagacttc tccttcttgt acttgcagat caaaatgatt
    17401 caatgctaca aaatctccat agcgtttgac taatttatca atttcgataa tcatcatgaa
    17461 aaactccttc caaatttctt gttgatctga ctataataaa gtgcaagaaa agaaactagt
    17521 gacgattggc taaaccacac atgacatttg tcacaatgaa ggagtctgct atgcataaat
    17581 ctcggctact caacgaaact agctttctac tggttatctc attgcttttg ctggtaagtc
    17641 aaggattcgt tgctatgcaa gttatctatt tattgatcgc cgttttattc actggtgtca
    17701 gttatttact gcctacaaag ccaaattgga ttttccaagt tattctattt cttttatgtg
    17761 cctttttacc tgatcttgtc ctgtttctgc cagcgacgat gcgagtgatc gcccctgaag
    17821 aaagccacct tgattacttg tttttgttag gttttttgtt gatttcgacg gttggtaatc
    17881 atccccccat caaggtcgcc tttgtcgtaa ttttgctgtt tttttccttt tacttacgaa
    17941 aaaaagatca acgaacgacc gatcttgaga aaaagtacat tgtctccaaa gatactcatt
    18001 gggaaaaaca acaagagtta tcggaacaaa ataaagcatt ggccctgtca caagaaacac
    18061 aattagcctt gcaaattgtg gaagaacgga accgaattgc ccgagacatc catgacaatg
    18121 tcggtcatct gctgtcaagt gcgattttac aaattggtgc gcttgaaatg atcaatcaag
    18181 aaccaaaact aaaagaacca ttagaaaatt taagtgccac cgttcacact gggatggatc
    18241 ggattcgtca aagtgtccat gacctccatg agacatcgat ctcttttcaa cactccttag
    18301 agtttctgat cgctgatttt ccttcttctg ttacgattga aggaaacttg tttgaaggat
    18361 tgaatgaaac gcagcaaaac tgcttcttaa tggtcatcaa agaagcctta accaacagta
    18421 tcaaacatag tcatgccaaa accgttgttt tacattttcg aacgttgccg gccttttatc
    18481 gcttgcaaat cacgaatgat ggcaccagcc cacaaaaaaa ttctgctccc ggtattggtt
    18541 tgaaaacgat gcgtcaacgt gttcaagaac aaaagggaca agtccatatt ttccaagaaa
    18601 accagcaatt tcagttaacg atcattttac caagggaggg acaccaatga taacagtagt
    18661 cattattgat gatgatccat ttgtgacgaa atcgttacaa accattttag aaagcacgaa
    18721 agagatccgt gtgttaggca ttggtcattg tgccaaggat gcgctagtct tatacgaaac
    18781 ccatcgtccc gatgttttgc tgacggacat ccgcatgcct aaacaaacgg gcatcgatgc
    18841 tgccaaagaa attttggcta aatttcccaa agccatcctt ttgcttttga cgacattcaa
    18901 agatgaagaa tacatccgcg aagcattttc catcggtgtc aaagggtatt taatcaaaca
    18961 gaacttgcaa gcgattattc cttctgtcaa atccgcttat aatggacagg ccgtatttgg
    19021 taatgaaatc atcgagactt tcactcaaat gatgaagaat aaaccgacga ttgatagtca
    19081 tgcttatgct tcttttagtg aacgtgagct tgcaatcatc aaagaagtag ctgccgggaa
    19141 aaacaataaa gaaattgcag atgccttgta cttgagtgac ggcactgttc gcaattacat
    19201 cagccaatta ctagaaaagc tagatttacg ggatcgcacg caattggcta tctattttta
    19261 tcagcatttg caataagtct tatctaacac aaaaaaacct gaatgatgct ccttcgcatc
    19321 attcaggttt ttttcacatc ttctattcaa ttgtcccgca aaccatttcc tcacgattga
    19381 aagaatcaag gattcgcagg gctgccagga ttcgttggat tgttcggcgt gttaggcgga
    19441 aaaataggcg ccttgtctaa cgttttttct agttttgatt cttcacgatc gtctcgttct
    19501 acatcaggac tttcattcaa tttgaattct ttttccatct catctaactt tttaaagtca
    19561 ttgccagatt cttttgctaa tttcgctaat ttatcttcgg tttctttagc cataaagtaa
    19621 cgcccccctt ttgatttcag cttagcatat cacactgaaa aaacaaaaga aaacgctaac
    19681 caacgatcat ttttagctat ttctcatctt taaaatcaag cattttttgc cggtataaaa
    19741 tcgtagggag aactgttttc cataccaatc atcggatcta ctaactggtt atcgatcact
    19801 tctgctgtaa gaacgaaatc tttagaaaca gcaggttcta cctcttcttt cttttcttct
    19861 ccaacaaatg aagaaaacag atctggttcc actgtttcca ttacttggtt tttcgtttca
    19921 ttcgtcccac tgtcatcttc tacaggtagc ttttctttta caacccgtgg ctcttttgta
    19981 tagttcccat cttcaaatgg atcatttccg accaaagaat cttcataagc tgctagtttg
    20041 gtccggagag tgatattcat tttatcatta tccatgatcc ttgatttcaa cgtagtcatc
    20101 cggcttgctg attcattcgc tacacaagtg acataggctt gctcttcccc taacaaaatc
    20161 aacagttcct tactgcgagt cactgccgtg tagagcaaat tacgctgaag catccgctga
    20221 tattgatgga ccatgggcaa aataaccatc ttgaattcac tgccttgtga tttatggatt
    20281 gagcaacaat aagaaagagt gattttattc cattcgtttc ttttataagt gacctcattt
    20341 gcatcaaact ggataactaa ttcatccact ttatcttcag aatcttttgc caagatgata
    20401 cccgtgattt ctcccatatc tccattgaat acattcaatt cgggagtatt gactaattgg
    20461 aggactttgt ctcctatccg ataaacggtg tcgttccaat gtacttcctt ttttccaggt
    20521 ttccctggat tgaatatctc ctgcatcatt ttatttaacg catcgatccc tgcaggtccc
    20581 cgatacattg gtgctaatac ttggatatct tgtgcacgga atcccttatt tttcgctttt
    20641 tccacaattt tagagatcaa gggttcaatt tgataagcat cacttcggaa ataggaacga
    20701 tctttttgat tctttgtgaa atcttttggc agacgtcctt ctttgatctc atgggcaaga
    20761 ggaataatac tagaaccatc accttgccga taaatctgat tgagttccat tttgggaatt
    20821 tgttcgattt ccaaaagatc atggagtact tgaccaggac cgacagaggg aagctgatct
    20881 ttgtctccta ctaagataac ttgcatattc gtcggaatcg ctttgaataa tgtgttagcc
    20941 agccatgtat cgaccataga catctcatca acaatcaaaa gtccgccttc taattctttc
    21001 gtactgactg aaggtgtttt ttcccgtcca ttcagcccca gaagacgatg gatcgtactg
    21061 gcaggcagac ctgtcgtttc gttcattcgt ttagctgctc ggccagttgg tgctgcaagt
    21121 agaataggaa aaatttcttg ggtatatttt gaaggatcca attccagtcc gttcaactct
    21181 gcaaaaagat tgacgattcc gttgatcaca gtagttttcc ccgtacctgg tcctcctgtt
    21241 aaaataaata gaggggaacg gattgcttca gcgatagctt gttcttgcga aggtccatac
    21301 tgtatgccga atattttttc gatacgccga atattcttct gcacttcttt ttcaggataa
    21361 tggatttctt ttttacgact gagaaggcgc tggatcgaat taccgatccc ccattcagaa
    21421 aagtacaaac tgttttcgta aagattagtc tcttcttgtt gtatctttcc ttcttcaaca
    21481 agttggataa ttacagtagc aacctgatca ggctggattt caattgggcg acttgtttct
    21541 aaaatacgga tcgtttgctg aagcaatgtc tccgccggta cgtatgtatc tcctgtttga
    21601 actgcatgct gaaaaatctg atgcaagata gctgctcgaa ttcttctcgg tgaatctgct
    21661 gcaataccga tttgttcggc tagattatct gcacgtttga acccgattcc ttctatgtct
    21721 tcgactagct ggtacggatt ttcttcgatg atatccaaag actcgttctt ataggcctgg
    21781 tagatactga aagctaactg actaccgaac ccgtaacgat tcagtcccat gatcacttga
    21841 tccatcccgt ggtttagccg aatcgtctca gcaagcattt ctctttttgg cttcgtcaaa
    21901 ccggagattt gttttaatac cgaaggatca gcaatgattt tttcgatagc atcttctccc
    21961 agcaattcta caattttttc tgctgttttc ttcccgattc ctgggaattt ttcgctggaa
    22021 agatacgcta cgatcccatt tgcagttgtt ggcttttctt gctcatatct ttcgagttta
    22081 agctgccttc cgtatttagg atgatccact aattctccat aaaaacgata agtctcattt
    22141 tcttgaatat cgccgaaact tccggtgact acaatctctt tttcacggta atcagctgtg
    22201 gtttcactga tgtgtaccaa aagcactttg taaaaattac taggattttg aaaaaaaatc
    22261 gcggcaactg taccaacgaa ataaggcatt tcttctggct gcatcggaag ccgcctcctt
    22321 cttctatagt aaaggttctg gtgctgtttc agttcctaaa aaatctgttt ggttccatac
    22381 tttcagttca tatggcaata atctgcaagg ctcttcagtc tctaaaatcg tcaaactact
    22441 attgaacaat ccacccattt cacgcaactc acttagctct tttcctgtca tccattgaat
    22501 ggctgcagtt aaagaagctc catgtcctac gaataaaagt gggccatctt tatgtcgggc
    22561 aaccgcatct gctacaacag tttcgatacg tgtgattgcc gattcaatcg tttctccgtg
    22621 aaaaggagaa ggatcataca gatctaaatg attacgcaaa ttcggcagat tatccggata
    22681 tttctggtac atttcatcga tcgactgacc ttctaaagta cccagcccta gttctcttag
    22741 cttatctgta taaaaaatct ctaccggacg agcaagctct ttgttgatac cttctgctgt
    22801 atcacgtgct cgctttgctg tactggaata aattgcttta aaaggaacat attttacctt
    22861 tttcccaaat gctttgattt cttcgtaact ttgtggcaaa agcggagaat cgccattgct
    22921 cccttgaaaa cgtctttcct gattccattg tgtcttccca tgtcgggtaa aatatagttc
    22981 catacgcagc aaactcctgt ctttctttta ttttactcta taataaactg tctaatccag
    23041 cttgtcattt tcatagtgat gcgtcaattc taaattaggg aatccttgct ggcgaagtgc
    23101 ttcatacact atcattgcag ctgtattcga taaattcaat gagcggacat gctcatcatt
    23161 catagggatg cgtaaacact tttcttcgtt ttcacgcata aatgtttctg gcaaaccggt
    23221 tgtttctttt ccaaataaaa agaagtgctc ttctccatca gcaaaatttt cgtcactata
    23281 tactttatgg gcaaattttg agatcaaatg tagtttacga ttacctaaaa atgttaaaaa
    23341 agcttctaaa ttactgtggt aagtaatatt tacatcattc caataatcca accctgcccg
    23401 ttttagatgt ttgtcatcag ttgaaaaacc tagtggctcg attaaatgaa gcggagtatt
    23461 cgtggctgca cacgtacggg caatatttcc agtgtttgca gggattaatg gttcaaataa
    23521 aacgatatga ttcatcagga tcattcctct catatttatg ttcgtttcta ttttctgaca
    23581 atttgatttt tgcataaaaa aaaacgtatc aactcggtgt cagtcactgc gagtcgttac
    23641 gttagtgaag ctcttctcac taacttttat cagaacagga acctgataaa aaccaatgaa
    23701 aaaacaactt gttgctaata taacaccctc aacttttttt tgcaacctct actcattaaa
    23761 aactgctaga aaaacgtttt ctcttagttc cttttaataa ttctatgtca attgtaatct
    23821 tcttcaaagg attcttatat aagtctgctt tgattcgctc ttctacctgc cattctaaag
    23881 gtgacatggc caaaagatct ttttgatttt cctctggtat ctcaaattca taggttacac
    23941 gctgacgttc agttaatttc attgcttcag caaacttggc tactacacgt tcattggaat
    24001 aactctgttt ttcaggttga ttcggataaa aagcctgacg tagttccttt aaataatttt
    24061 cttgtggcac caccttcaac acttctccat cttctgagag tacacgttgg aattcttggt
    24121 aatgagaagg cgaaaagata tttaaaaccg cgttgaatgt ttgatcagca aacggcaaat
    24181 tggtcaaatc agctacacac caaaatgctt cggcagattg ttctgtagct gcgtagactc
    24241 cttcttttga aatatcaaat ccaacagcat tttgaatctt tttcaatttt gaaagttcat
    24301 tcaagaagct accttctccg cagccaacat ctaatagcga gacttcttca ggtaaatgct
    24361 gcgcaatagt ctcaataacg ggctgataca tgccagattg gatcattcta cgccttggaa
    24421 cgaacattcc ttgatcgtat tctgttttaa ccgcatggga taaaaaataa agtgttcctt
    24481 ttttggaaag gtcaaaacgg tgattatttt tgcatatcaa gccattatca gactttttta
    24541 cttcttcttg gcaaaccgga cagcggaaca gctgatgatt ttctgccaaa aactggcgtc
    24601 ctcggtcaat tttttttaac atagttcccc ctcatttctg ttttatctta ccgttagatg
    24661 ctcactttgg caactgctaa aaatatgata caatgcctag aagaaagtga aagataaagg
    24721 agaagattag atgatcaaag aattttgcgc tgaaaactac acatctattc ctctagcaat
    24781 tgctagagga gccgatcgga tcgaattgtg tgacaactta gcggtcggtg gcacaacccc
    24841 gagtactggt gtgatcgaag aaacgttaag ttatgctggg gaaaaagata tccccgtaat
    24901 gacgattatt cgtcctagag ggggcgactt cgtctataac gatatcgaat tgaaaatcat
    24961 gcatactgat ttgattgaag caaagaaatt agggacggat ggcgtagtat ttggttgtct
    25021 tactccttca ggctggctag acgaagaggc cttagaacta ttgatcgaaa cagcagaagg
    25081 gttgcagatc accttccata tggcttttga cagtattcct gaagatcggc aattcgaagc
    25141 aatcgattgg ctggtagaac atggtgttga ccgtatcttg actcatggcg gtcctgctga
    25201 caaaactatt gtagaaaatt tcccacggct taaagaattg attgcctata cagatggccg
    25261 cttaatcatt ttgcctggtg gtggtgtcac tgcagagaat gcagcacatg tagctaaaga
    25321 attgaacgta agcgaagtac acggaaccaa aatcgtggat cttcagttat aatcttataa
    25381 acaaaaaaac atttgcaaca ggcataatgc ttattgcaaa tgtttttgtt tttttacata
    25441 aaatagccca taaataaaga agcaatattg aaataaatca gtacactcgt caaatcgctc
    25501 aaagtcgtga taaagggacc gcttgctact gctggatcaa atcctagacg atccattagc
    25561 atcgggatta aactacctgc aagattcgcc actgtgattg caaatagcat agccatccct
    25621 atgacaaacc ccaagataaa attatgtttc caaaaaccta cgattagaaa aatcgtgatc
    25681 cccgtaacag ctcctgtgac aacgccggtc aagacttcag ccaaaacaag tcgaacaaat
    25741 ccatgatctt tctcatcaga aatcgcaagt cgacgaacag ccacagctag cgactgggtt
    25801 ccagcattac cggcagttcc tgtaatcagc gaaatgaaga ctgctaaaat actcgcttca
    25861 cttaccaagt cttcatagtg actgattaaa gaagcggtcg acatacctaa aaacaacaga
    25921 gtgatcagcc aaggcaaacg tcgagacgct gcctttactg gattttcatt aatttcttcc
    25981 acattgaccc cggcaagtcc tgaataatcg ctagcagctt catcatcaat aacgtcgatg
    26041 atatcatcga ctgtgacgat tcctaacaaa tgatcatcat aatcggtaac aggaagagct
    26101 aagaagtcat aatctctaaa tgtttgtgcc acatcttctt ggtcgtcgcc tacatgaact
    26161 gaaagcactc gttcgctcat cagatcagaa atcattgcgt cgtcatcatt cacgattagg
    26221 tcgcgcagag aaataacccc tactagctga ccttcttgat taacgacata gatgtaatag
    26281 attgtttcag ctacatctgc ttcgttcttc agcacgtaca tcgcagagcg aacagtttga
    26341 ttggctacga tcgaaacaaa ttcagttgtc atgatcgcgc cagccgtttc atcctcgtaa
    26401 tggagaagtt ccttgatttc actggcatcg tctgccgaca tcaaacttaa atatttagca
    26461 atttgctttt tatccagcgt attaagcaag tcaacagcat tatctgtata catctcagaa
    26521 agcatatcag ctgcatagct aggacgcatt tcatctaggt attctttcat atgttcgttg
    26581 tcttcttcaa tcacatcgaa catatccgct aattctttag gcgacagata ggaatagatc
    26641 agctgcctgt tctcttctgt aagagattgg tagaattgcc cctgctcata gatatgcatc
    26701 tctaaaaaac tgtttctgaa tgcctgaata ttattttccc ttaaatcttg agtcaactgg
    26761 gcaaatcttt cctctaattc ttgattttca tccaaaccaa tctcacctcg ccttattctt
    26821 caaaccgtct cacaactgcc tccatgtctg cggctaaagg ctgcttgaac tccagcaact
    26881 cttctgtaaa tggatggtaa aaacgcagat cataacaatg aagggcttgt ctttttattc
    26941 ccagatccat tcgcccgcca tacatatcat caccgagcag ggcacagcca attgcggaaa
    27001 aatgaactct tatttgatgt gtccttcctg tatgcagctg gatatcaact aacgcgattt
    27061 ctgttagtcg cttctctatc cagtactcag tctgtgccga acgtccttca tcagagacca
    27121 ttcgcttcaa tagcgaagaa taatctcggg cgatcggcaa atcgatatgt ccatgtgccg
    27181 ataaatctga aagccttcca ctgactaagg cctgatattt tttcacaaac ttcttttcac
    27241 gaagttgcat atctaatttt gcgtgcgcaa atccatgctt tgcaaaaagc atcaagccag
    27301 atgtatcccg atctagtcgc gtcaccacat ggattacttg gttttcgtaa ccttgttttt
    27361 tataataagc ctttacacga ttagccattg tcccatttgg atggtattgg gcaggaatag
    27421 acgaaacgcc tgctggttta tttactacca gaacgtgttc atcttcatac acgatatcga
    27481 tcggtgtttc atccaaaagt acggtttcat gttctccttc agcaggaatc acaatggtta
    27541 ccttatcatc cttagctaga gaaaacaaga cattttctac ttgatcatta actttgattt
    27601 gaccgccttg aaattttatt ttcgctaaca gaccctttga gatccctttt tcttttaaaa
    27661 aatattttac ttgctgagaa tgatctttct cataatccca tgtaaactgc atatcaatcc
    27721 tcactaataa aggcatcttt cacacgatgc caaaaatgca tatgtcgata agaagcaaaa
    27781 tggatccgtt cttctgctat tcgataataa attgagcgga tatttgcttt tgcgacattc
    27841 aattgatcaa ccgttacaag ataatcttct gtattttcca attttatttc tagccattca
    27901 tcatgggcaa tcacgatcgg agaacccaaa gtacgaaaca ctcgattatt cagtgaagca
    27961 atctctgcca actgaaaggc attgatacta ggatggatca cagcaccgcc gacactttta
    28021 ttatatgctg tcgaaccagt aggcgtagaa atcgtcaatc catctcctct aaaactttca
    28081 aacagctcgt ttttgatgta tacatcggct accatcgtac gattggcccg tttaatcgtt
    28141 gattcattca gtgctaaaaa atgctggtcc ggtgtttcat ccaaataact gatccgaaca
    28201 tccagcaagg gataactaac actttgttcc cggttcgttc gtaagctgtc tactagttct
    28261 tccagctcgt aatcccgcca atccgtgtaa aaccctaaat gacctgtatg cacgcccaag
    28321 aaacgcacat cattcagaca atgactgtag tgatggaacg ccgacaataa tgtgccgtct
    28381 ccgccgactg agatgaccaa ttctggctca tgatcatctc gttgaatccc tgcttgttct
    28441 aaaagtgcgt tcaaacgttc tgtcacttct atagtctttg cttctttatt atgaacgatc
    28501 gctactttca tcccctgcct cctctctttc gctttctttc ttcaaaagcg ttcgatagta
    28561 gacatcatct gattttcctc ttccatggga gaataaccgc tgtgcttctt ggatttcttc
    28621 acggatactg gacatctctt catccaactg ataggcagct tctgccgcac gctgaagtcg
    28681 ttcactcatt tcttctggaa aaacgccttg atatttataa ttcagcgaat gctcaatcgt
    28741 tgcccagaaa ttcatcgcaa gagtccgaat ctggatttcc gccaaaattt tcttctcacc
    28801 gctgatcagc tgtacaggat actcgatcac cagatgataa gaacggtagc cacttgcttt
    28861 tttatttgtg atgtaatctc gttcttggag aactttcatg tcttttcttt tacggatgat
    28921 ctcaacaact tgatggatat cttctacaaa ctgacacatg atccttaacc cggcaatatc
    28981 agacatttct tcttctaaac gtcccattgg aatatgccgt aattgtgatt ttgttacaat
    29041 actatcgact ggcttgactc tcccagtgac aaattcaatc gggacatgtc gattttgttc
    29101 acggaactgt ttgcggatcc cccgcagttt cactttcaat tctgatacag tttgttcata
    29161 tggtgctaaa aatgcttccc aatctcgttc catagctgat acctctactt cattaacatt
    29221 cattgtttat tctaccatat atgcaaagtt ttttcgtact cttgatttat gcattgacca
    29281 aaaaaagaaa attaggcaaa atagacaaaa aggagtcgat caaatgacac aaatgcttga
    29341 aatcgaatat aaatctatgc tgacaaagca agagtacgag atattcatcg ctcactatca
    29401 attaacagat aaagatttcc gtgtacagac taatatttat ttcgataccg ttgatgaaca
    29461 gctgaagaaa caaaattgtg gcctacggat ccgtttcgtt ggtcatcaag ctgaatatac
    29521 attaaaaaca cctgctgaaa aagggcgtct cgaaacaacg gataccttta taacagaaga
    29581 agcagaagca tttatcttcg aaaaacgtct gcctcgttca ggcgcagttt ttcaaaaact
    29641 ttctgatttg aatatcgatc ctgcttcatt aatccaaacc ggaaagctta caacaaaacg
    29701 agctgaattc cctattgaag aaggactttt agctattgat gaaagctggg gagacaatct
    29761 acacgatttt gaactggaac tagaggtagg ggatgcatct gtgggaaaaa ttgcttttat
    29821 aaatttttta aaacgttttt ctttacctta tcgtcctgct aagaacaaga tccaacgtat
    29881 gcttgaagct aaaaattaga aacctttcat tttcacaagg cacttgagtt cattttttta
    29941 cttttttctg ttaagttata ggatgtgaaa gaatctagtc actagttgta tagaatctat
    30001 tttgggggag attcatatga tcgaaatcta tttatttgtt aatcctttag gggaatactg
    30061 tttcgaaatg gaacaacagt tattacaatt tatcgaagaa gagtacggga caccatcaaa
    30121 agaaaagatg caatttcgct ttcttccttt ggtcaatttg cagacaattg gggatgtcat
    30181 gcaacaaaaa ggaatttctc agaacaatct tgaggaacgt aatcacttat tctcaatgac
    30241 ttattctgca gcattggact gcaaagcggc gcagtttcaa ggaaagaaaa aaggccgccg
    30301 ttttttgatc aaattgcaag aagcagtcgg ctgtaatgac ataccatatt ctcaagaact
    30361 tgctgaatcc atatttgaag aaatcggcgg cgatttgaat atgtttaaag aagatcgtca
    30421 gtctgacttc gttaaagaag tattcttttc tgatcaaaaa attgctcggg aaatgggcat
    30481 cacaaaacac ccttctgcag tcgtttacaa ttatgcctgc gaccgtgatt ttggtgtttt
    30541 agtagaagta aaaggtacaa gtagcctttg gagaatcaca gagctgtgta aaacaacaga
    30601 taactcatat caagttttta atggtgaaca tttacataca aatgattctc agttccattc
    30661 aagaactagc catttacact tgctttctaa ttaaaa
//
LOCUS       DAZUMM010000027        29571 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000027 DAZUMM010000000
VERSION     DAZUMM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.027"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..1342)
                     /gene="purB"
                     /locus_tag="ACG8E6_001173"
     CDS             complement(47..1342)
                     /gene="purB"
                     /locus_tag="ACG8E6_001173"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFS5061605.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1355..2479)
                     /gene="purK"
                     /locus_tag="ACG8E6_001174"
     CDS             complement(1355..2479)
                     /gene="purK"
                     /locus_tag="ACG8E6_001174"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFS5061606.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2472..2960)
                     /gene="purE"
                     /locus_tag="ACG8E6_001175"
     CDS             complement(2472..2960)
                     /gene="purE"
                     /locus_tag="ACG8E6_001175"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFS5061607.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3376..4683)
                     /locus_tag="ACG8E6_001176"
     CDS             complement(3376..4683)
                     /locus_tag="ACG8E6_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFS5061608.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4686..5267)
                     /locus_tag="ACG8E6_001177"
     CDS             complement(4686..5267)
                     /locus_tag="ACG8E6_001177"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFS5061609.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5383..5480)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5839..7131)
                     /locus_tag="ACG8E6_001178"
     CDS             complement(5839..7131)
                     /locus_tag="ACG8E6_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5061610.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7128..7751)
                     /locus_tag="ACG8E6_001179"
     CDS             complement(7128..7751)
                     /locus_tag="ACG8E6_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061611.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7754..8590)
                     /locus_tag="ACG8E6_001180"
     CDS             complement(7754..8590)
                     /locus_tag="ACG8E6_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5061612.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8607..9494)
                     /locus_tag="ACG8E6_001181"
     CDS             complement(8607..9494)
                     /locus_tag="ACG8E6_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5061613.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9510..10793)
                     /locus_tag="ACG8E6_001182"
     CDS             complement(9510..10793)
                     /locus_tag="ACG8E6_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5061614.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11476..12159)
                     /locus_tag="ACG8E6_001183"
     CDS             complement(11476..12159)
                     /locus_tag="ACG8E6_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS5061615.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12177..12794)
                     /locus_tag="ACG8E6_001184"
     CDS             complement(12177..12794)
                     /locus_tag="ACG8E6_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS5061616.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12804..13628)
                     /locus_tag="ACG8E6_001185"
     CDS             complement(12804..13628)
                     /locus_tag="ACG8E6_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFS5061617.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13621..14181)
                     /locus_tag="ACG8E6_001186"
     CDS             complement(13621..14181)
                     /locus_tag="ACG8E6_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061618.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14355..15311
                     /locus_tag="ACG8E6_001187"
     CDS             14355..15311
                     /locus_tag="ACG8E6_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5061619.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15616..16572)
                     /locus_tag="ACG8E6_001188"
     CDS             complement(15616..16572)
                     /locus_tag="ACG8E6_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFS5061620.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16676..17278
                     /locus_tag="ACG8E6_001189"
     CDS             16676..17278
                     /locus_tag="ACG8E6_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5061621.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17366..18865)
                     /locus_tag="ACG8E6_001190"
     CDS             complement(17366..18865)
                     /locus_tag="ACG8E6_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFS5061622.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18881..19405)
                     /locus_tag="ACG8E6_001191"
     CDS             complement(18881..19405)
                     /locus_tag="ACG8E6_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFS5061623.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19703..20452
                     /locus_tag="ACG8E6_001192"
     CDS             19703..20452
                     /locus_tag="ACG8E6_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS5061624.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20443..21228
                     /locus_tag="ACG8E6_001193"
     CDS             20443..21228
                     /locus_tag="ACG8E6_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5061625.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21197..21913
                     /locus_tag="ACG8E6_001194"
     CDS             21197..21913
                     /locus_tag="ACG8E6_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5061626.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22015..23034)
                     /locus_tag="ACG8E6_001195"
     CDS             complement(22015..23034)
                     /locus_tag="ACG8E6_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFS5061627.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23053..24054)
                     /locus_tag="ACG8E6_001196"
     CDS             complement(23053..24054)
                     /locus_tag="ACG8E6_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFS5061628.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            complement(24333..25481)
                     /gene="nagA"
                     /locus_tag="ACG8E6_001197"
     CDS             complement(24333..25481)
                     /gene="nagA"
                     /locus_tag="ACG8E6_001197"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFS5061629.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(25557..26393)
                     /locus_tag="ACG8E6_001198"
     CDS             complement(25557..26393)
                     /locus_tag="ACG8E6_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5061630.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(26577..27794)
                     /locus_tag="ACG8E6_001199"
     CDS             complement(26577..27794)
                     /locus_tag="ACG8E6_001199"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS5061631.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(27943..28104)
                     /locus_tag="ACG8E6_001200"
     CDS             complement(27943..28104)
                     /locus_tag="ACG8E6_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061632.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            28177..28260
                     /locus_tag="ACG8E6_001201"
     tRNA            28177..28260
                     /locus_tag="ACG8E6_001201"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28211..28213,aa:Leu,seq:gag)
     gene            complement(28500..>29571)
                     /locus_tag="ACG8E6_001202"
     CDS             complement(28500..>29571)
                     /locus_tag="ACG8E6_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061633.1"
                     /translation="VFEKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFS
                     LLTFYPFFQLLRGEEINRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRY
                     NYKLSVFSIISLAELIFSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKP
                     FFLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNG
                     NKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTS
                     IFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKN
                     EFMQFGLTVLGICTFIITVINR"
BASE COUNT         8843 a         6304 c         5064 g         9360 t
ORIGIN      
        1 tatttttgct attctatttt aattgaattt tgcaactcac tcttctttat tccaaaccta
       61 ctcgtttgaa tatcacgtca acgtttttca aatgatgatg ataatcaaag gcatcgtcta
      121 tttcttcttt agataatacc gatgtgatgg ttggatcttc ttctaacagc gggcggaact
      181 gcacttggtt gtcccaagcg tacgcagttt ttggctgaac aagatcataa gcttcttctc
      241 ttgtcattcc cttatcgatc aatttcagca taacgcgctg gctataaatc aaaccaaatg
      301 ttgcagacat attccgcttc atgttttcag gaaagaccgt tagattttta acgatattgc
      361 caaaacgatt taacatgtaa ttcaataaaa tcgtggtatc aggcaagatg attcgttctg
      421 ctgaagaatg agaaatatct ctttcatgcc ataatgtgac attttcatag gcagtgacca
      481 tgtgtccgcg caccactctg gctagaccag ccatattttc tgaaccgatc ggattccttt
      541 tgtgcggcat agcagaagaa cctttttgac ctttcgcaaa aaattcttct acttcacgtg
      601 tttcggattt ttgcaatccg cgtatctctg ttgcaaaacg ttcaatactt gtagcaataa
      661 gcgccatcgc tgagaaatat tcagcatgta gatcccgagg gagaacttga gtagagattt
      721 cttgtggacg gatccccaat tgtttacata catactcttc aataaatggc gggatatttg
      781 cgaacgttcc cactgctcct gagatttttc ctgcttctac tccttttgcc gcatgttcaa
      841 atcgttcgat gttccgtttc atttctgaat accatgtggc aagtttcaat ccaaaagtag
      901 ttggttcagc atggacaccg tgtgtccgac ccatcatcac tgtatatttg tgttcttttg
      961 ctttttcgcc aatgatttcc gtaaaacgtt tcaagtcttc ccgtaaaata tcatttgctt
     1021 gcttgatgag atagccataa gcagtatcta cgacatccgt acttgttaga ccataatgga
     1081 cccattttct ttcttctccc aatgtttcag aaaccgctcg tgtaaacgca acgacatcat
     1141 ggcgtgttaa tttttcgatt tccaagatcc gatcgatatc aaatgatgca tgctcacgaa
     1201 tttttttcac atcttcttgc gggatttcac caagttctgc ccaagcttca tctgctaaaa
     1261 tctcgacttc gagccacgca cggtatttgt tctcttccgt ccagatattc cccatttcag
     1321 ggcgtgtata acgttctaac atgttttttc tcctctaatc ccaaatattc gtttgtttga
     1381 tttcttccag cgtttgctgg atgtcttctg tcaagatagt gatatgtccc atcttgcgac
     1441 ctttttttgc ttgttttttt ccatagaaat ggaattgcca gtctggctta ttctcaatca
     1501 agcgatagct ttccagcagt tgttctccca aaacattgac cattactgca ttgcttatca
     1561 aacgtacttt ttctcctaat ggccaaccac aaatacctcg aatatgggca tcgaactgac
     1621 tcatagaaca agcttcaata gaataatgtc cagaattgtg aggacgagga gctaattcat
     1681 tcacatatag cccgcctgtt tttgttaaaa acatttctac ccctaaaaca cctgaaaggc
     1741 tcactgcttc tgcaatcaca cgggcaatac gctcagcctc ctcgatcaca tcctgatcaa
     1801 tcgcggctgg agcaatcgtt tcatgaagaa tattgttgtg gtggatgttt tccacgaccg
     1861 gaaaagtcgt gaaatctcct tgaccatttc ctgccaccat gatcgaaatc tctttttcaa
     1921 acgggatcca tgcttctaaa acacaggttc cttctcttaa aaggtccatg gcaggagcta
     1981 gatccgcccg acttttcaaa acatactgcc ccttgccatc atagccgcct cgagtggttt
     2041 tcaaaacaca aggatagccg atcccgtcaa tcgcatcttg gatatccgtc ggactgacga
     2101 tcgttgcata aggagcaatc acgatgttat tcgtttccaa aaaagatttt tctaacaggc
     2161 ggtcttgcgt aatcgcaagc aagtctgtcc cttgaggaat aaatgacatg ggtaaaatcg
     2221 catttaacgc ctctacactg acgttttcga attcataggt aatgacatct gttcttctgg
     2281 caagctcttt cagcgcaaag gtgtcatcat agacactttc aatgtgccaa tctgcaactt
     2341 gtgcggctgg acaatcgctc acaggatcca gcacacctac tttaaagccc atttcttttg
     2401 cactaatcgc catcatacga cctaattgac cgccaccgac gatcccgatc gttgaaccag
     2461 gcaacaaagg tttaaccaag ttgatcacta ctttctataa cagttttagc caacattgaa
     2521 cgtctttcag ccaatttagc ctccaattct aaatcataca tggaaagcat ctgtatcgca
     2581 agtagtccag cattaattgc tcccgctttt ccgattgctg tagtagctac aggtactcct
     2641 ccaggcattt ggacaatcga aagaagcgaa tccaaaccat ttaatgttcg tgattggacc
     2701 ggtacaccga taaccggcaa agttgttttt gatgcgacca tacccggcag atgcgcggct
     2761 ccccctgctc ctgcaataat gattttgatt cctttttttc gagcgttttg tgcatattca
     2821 aacatcaaat ctggggttcg atgagcagaa acgacctttt tttcataaga cacaccgaat
     2881 tcatccaata tttgacatgc ttctttcatt gtttcccaat ctgaagtact ccccataatc
     2941 acagagattt ttgctgtcat tgtttttcct cccagttttt ctacattcat attttaacaa
     3001 tcactttttt tgatgtcaaa gaaatttcga atgttaacaa agaaaaacat tttattgttc
     3061 gtgtttatta aagataaaaa aaaggaatgt gacaaaagtt aggtcacatt cctttttatg
     3121 gataaacggt gttctcctgc caactcctcg gtcacaagga acaatattca acaaacatga
     3181 gaagcttttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
     3241 ttccttcaac ggtgttcaat cagctgaccc tcggtattag ctcaaacaaa cggcaaaata
     3301 cgaggaacta tttttccgtt tgtttctcta acaatcaggt caaaacgctg atttcacaac
     3361 ctttcttttg ctctattact tcttgaaggc ttcttccaat atttcttcat cttttttcaa
     3421 tccattaaag atcaaattca atatgatagc cgtaatgcta ctcattacga tgccatttcc
     3481 agtgaacatt tgcaatgttt gcggcatttg actgaataat gtcggcgtaa cattgaaacc
     3541 cagcccaaat ccaacagaaa cagcaacaat caatagattt ttatcattgc tgaaatcaac
     3601 ttccaacagc atacggattc cttggactgc caccatcccg aacatgacta acattcctcc
     3661 accaagtacc ggctcaggga tgatttgtgc taacgcaccg attttaggga ataatcctaa
     3721 aacaatcaag aagaatgcag agaaatagat gggttttctt gttttgattc ccgaaagttg
     3781 aaccaatccg acattttgag aaaagccagt ataagggaat gtattgaaaa taccgcctaa
     3841 gataacggca agtccttctg cacgatatcc ctttttcaat tcttcttctc caacattttt
     3901 cccagtaatg tcaccaagtg caaaatatac acctgttgat tctaccatgc tgaccatgga
     3961 aatgatgatc attagtaaaa tcgaccatac gtcgaaagtt ggtttaccaa aataaaaggg
     4021 ttgtggaaca tggaacaacg gcgcatctcc aatcgcgctc agatcaatca ttcccatgaa
     4081 agcagctaag atgcttcctc cgactaaacc aatcaatacc gcgattgaac gaatgaaccc
     4141 ttttgccagc atttgcacgc ctacaattag gagaatcgtc acaaatgcaa gtagcagatt
     4201 tgttttgtca ccaaaatcag ctgctgttgc attacctcca cccattttct cgattgctac
     4261 aggaatcaaa gtcaaaccaa taacggtgat aaccgttcct gttactatag gagggaatag
     4321 ttttttaatt ttagaaaaga caccagaaac taacacaaca aagatcccag aagcgataat
     4381 agatccgtag atagctccta ctccttcatt ggttccgatc aagatcagtg gagctactgc
     4441 ttggatcgca catcctaaaa cgacaggcaa gccaattccg aaaaatcggt taactgtcaa
     4501 ttgcaataat gtcgctaatc cacacataaa gatatcaatt gaaatcaaat aagtcatctg
     4561 ttcttgatta aagcctagtc ctgtaccgat caaaagaggc accgctactg ctcctgcata
     4621 catcgctagc agatgctgca gccctaaaac cgcagctttt ccattttgtg tttctttttc
     4681 cacgattatg cgtcctcctc aagaaactca acttgtccat tcgtcaagga agcaatacgc
     4741 gcaagagaaa cgactctcaa tcccatttct tcaagtaact gacgtccatc ttggaaagac
     4801 ttttcgatga cgatcccgat tccttctact tgcgctccag cttgctggca taattcgatc
     4861 aatccttttg ccgcttgtcc gtttgccaag aaatcgtcga taatcaatac tttgtcttct
     4921 gaagataaaa atttacgaga aatagaaatc gtacttgtca cttgtttcgt aaaagaataa
     4981 acagaagctg tcaataattc ttcatccatc gtcaaacttt ttgcttttct agcgaagatc
     5041 attggaacat ctaattctct tgcagcaaat aaagctggtg cgatcccaga agcttcaatg
     5101 gtcacaactt tcgtgattcc ttgttcttta aatacttctg caaaccgttt tcccatttgt
     5161 tccatcaata cagggtctac ttggtgagtg acgaagctgt cgacttttaa tacgccctcg
     5221 cctaatatac gtccgtcttt ttgaatacgt tcaactaatt ctttcacttt atttgctcct
     5281 ttatcattta ttaataccga aataaccgct tttgtgcaaa aacctactat gtttctgtta
     5341 aagaaaaaca ccctccactt tatcataaga aaagaagggt gaaatagcca atcttttact
     5401 tatagtccag catttacggt actggctaga aactgtcatc catattatga cgatatataa
     5461 gtaggtatgt aaaactaata atagtgtaaa ttcattttct tgcaactgtt ttgcacgtga
     5521 aggcagtttg ccatatttga ataaaaaatg ttgtttcaaa tattgaaaac agtctaacac
     5581 tactttctga aactgacaac gtggtttact tatttttttg taaaatctaa tgatttttaa
     5641 tgcattattc ctttttgttc gggtttaaat agttattttt attttctcct atctaaaaaa
     5701 gcagcccaca taaaaagaag gcatgacaaa aagcatgtca tagccccttt ttccgaataa
     5761 atagtgttca aaagacagcc tatcggcaat aaggcaagat tttataaact acgggaagta
     5821 atttccgtaa atctattctt attcttttaa gctgacaagc tcgtcaatca ctactgcttt
     5881 tcctgaagca atagaatgat ttgctgcgat tcctgtcaaa atcgacaaaa taccatctac
     5941 gtgagttgct gcccgattcc acggatcttc ttctttttct tcaccaaata gatcatctaa
     6001 taaacgcgga tctcctccgc catgtccgcc cgcttcttgt tcgacgatta cctcatatgg
     6061 ctcaccgaac atcggataga catggatgga ctgaagaacg gctgctcctt catccttttt
     6121 atctccacct gcattgatgt aagacttttc gatcactttc atttcgatcc gtcctttgct
     6181 gccattgaaa gaaacattga atccttccca cggcatatac gcgtttagcg aatacgtcaa
     6241 aatcgcacca gtttcatatt tgaccgttac gcctagtgta tcttcaatag agatatcatc
     6301 ggaaaataca ctgcggtcac gaatatatcc actgtctttt tctgcttcta aatacatttc
     6361 tcgcaattcc tcattggctg acaggtctat agcaaatgga tcagctttag ctagttcgct
     6421 tccatagcat cgactgtaaa atatttctac tccgcgtttc tccgcattat gcatcccata
     6481 aaagtttaat ccaccgaatg caaatacctt ttggggacgg ctgttcaacc aaaaattcac
     6541 tagatcaaaa tgatgggtag atttatgaac aagaagcccg ccgctattgt gcttatttcg
     6601 atgccatctt ctgaaataat ctgccccgtg ttgcgtattt aacagccatt cgaagtggat
     6661 agaaaacaca tcaccaatca catcactttc tatcagttcc tttatcttcg tgtgatacgg
     6721 tgcataacga taattaaaag tgactcgcag gcttcttccc gtctgttttt ggaccgttat
     6781 tatttcttga gctttttcct catcgatagt cagaggtttt tcgcaaataa catcatatcc
     6841 ttttttcatt gcacgaatga tataatcatg atgcgttcga tctactgacg taataatgac
     6901 ttcagccggc cgtacgtcat ccagtagttg ctcaaattgt gatggcatac atgtctttac
     6961 ttttttctgt ccatacttgt tcacaagtat ccgatttgca taatccatcc ttgtttgaga
     7021 tacatcacaa aaaccgacta gttcacaagt ttgatgatac gtagaggcaa ttgcctcata
     7081 aaaaaaacgc gctctcccac caagtccgac ttgtacatat tttttcttca tttaaagtca
     7141 ttctcctttt cttttaagca aaatactgtg acaaatggca gccctggata cagtaatgca
     7201 gtgagtaaaa aatcatgctg tatacaccag aacagtccat acaaaaggca taaataccac
     7261 agaaacccct tgacgttttt tgacaacagc cagatattcc ttttgatcaa ataaacaaga
     7321 ctatctgctt tatcttcttt gcagatatca attagcgtac taaaaaaact atgggataca
     7381 aaaagcaaca gacacaatag aaaaggaatc agataccaag ctccaatctt tcccactaat
     7441 aaataccaat caactgcaaa aataatgaca aacagctgaa acaacgtaat gatgaaacac
     7501 atccgtttga tttttccttt cctccaacat accttttttt ctgccaagta atggacagat
     7561 agataataag aaggatacat catcaaaaaa taaatcagat agacaacacg agcagacagc
     7621 actaatccta aagtgaacaa acatcctaga actaaagcga aaagagcatt actgataaga
     7681 ctggttaaaa aaatccgaca gacatagtag ttgagcttgt aaagaaacat ctcttctatt
     7741 ggctttttca tattcagccc ttcagaccgc ttgtactgat cccctggaca agatatttat
     7801 tgaagaataa gaaaatcaga aaaaccggta gcagcgacaa cgtagacatc gcaaacatac
     7861 tcgcgtaagt cgtactagat ccagcgtctg caaataattt gatggcaatc gataccgtgt
     7921 aagatctcgg cttgtttaaa taaatcaatg gtcccatgaa gtcgtcccat ttccaataaa
     7981 actggatgat gatcgtcgtg atgatcgaag gtttcagcaa aggaaaaata attttggtaa
     8041 agacactata cttgctgcat ccgtcaataa tcgctgattc atctaactct ttggggatgc
     8101 tgaccatgaa ttgcatgatc tggtaaatga aaaatgcttg tccaaaaaaa tgtggtaaaa
     8161 tcagcggcac atacgtccca accaaattca gccgattgta aataataaac tgcggaatca
     8221 aaatcacttg tgcaggaatc atcatcgtta ctaacatgac gacgaaccag aatctttttc
     8281 ctttgaaatc gattctcgat aatgcgtaag caatacaagt cgagctcaga actgtcccta
     8341 tcgtcgaaac tgcagagatg acaaacgaat tcctaaagaa agtaaaaaat gaaattccag
     8401 caaaccccgc ccatcctttt gcgtaatttt cccattgaaa agtctctgga atcaaattca
     8461 gcgaaccact cataatctct tgatccgttt taaaagaacc tgcgatcatc cataagaccg
     8521 gataaatcat gactagacct aatagcaaaa taaaaccgta ttgaaaccat ttcgttactg
     8581 tattattcat ttcttcctcc tctcattcac acaacgaccc atttttttga tgatttgaaa
     8641 atcagaaagg tgacagctcc cataatcaac agcatgatcc acgaaatagc actagcatac
     8701 cccatagaat taaaagaaaa agcttggtta tacacataaa gagcataaaa gttcgtggca
     8761 tcattcggac caccttgggt aatgataaat gcctgcgtaa aagtcataaa cgccgcgatt
     8821 gtctgcataa ccaagttata caaaatgatc ggcgaaagaa gaggtaacgt gatcttgtaa
     8881 aacgtctgcc atttgttcgc tccatcgatc tctgccgctt cataatacga gcctgggatc
     8941 tctttgatac ctgcagcaaa gatgatcatt gccgaaccga attgccaagc agacatcaaa
     9001 acaagaggga tcatgaccag tttttgatcg ctaaaccagg aaagcgcagg tgcgcctaat
     9061 cgttccagga aaatattcac taacccgttt ttagaaaaca actcccgcca aactagagcg
     9121 atagaaatac tgccaccgat caaactaggc acataataca aagaacgaaa gaaagtcgtt
     9181 cctttgatat tcctcgttaa aagataagca atcaatagac caaagatcag tttcacaggt
     9241 acagatgcaa atacatagag aaaagtgact cggatggagt tcaaaaaacg ggcatcactc
     9301 agcatcttta tataattatc caagccgatc catgagactg gatttcccaa acgataatcg
     9361 gtgaacgaat aataaagtga caagatcatc ggtatcactg ttatcaatag aaatcctagg
     9421 atgaatggag aagcaaatac gtaaccgacc acattgtctt gatccaagaa ccgttttagt
     9481 ttttcttttt tcatgccttc ctcctgctgc tatcctagaa tgtttgacgc aaactcataa
     9541 agactctctg ctgcttcttc tgctgttact tcttcataaa tgactttttc aactaaaaga
     9601 ttgtattggt cttgaatttc cggattcaaa gcactatcaa agacattcac ttcgccgttg
     9661 tccatatcgg aaatcatttc taaataatca aatgtcgctt gcaaagttgc atcatctgca
     9721 cgagcgagct gttctcgaat attcgacatg attggtacac cacgttctcc ttttaaaatt
     9781 tcattggctt cttcactgtt ttgaaagaaa tcaataaact tagcagcttc ttctttatgg
     9841 gctgagcttt ttgccatact cagcatttgc gaagaacgta aagggatacc agattctcca
     9901 tctgcttttc tgcgaggtgg attaatcaac tgaatctcca tatcttcagg agcccctgac
     9961 aatatttctg tcaactggtt acttgataga aacaccattg ccgcttctct atccctaacc
    10021 gcctgaaaag acagatccgt cgtacttgca gctccaccac ctggttctgg atgggcccct
    10081 ttgtctacca gatttttacg cagctggaaa aaatctgtta gatagtctgg attttcaaat
    10141 cccaagctgt tagatagatc ttcccgatta taaaaattca atcctttctc aacttgtggt
    10201 atttgaataa tacagccagc tataaaatct tcgaatcttg aaatagcata gattcctaat
    10261 tcttcagtga tttgatagct gatttgttca aattcatccc atgtccaatt tgaagctggt
    10321 tcagggatat ttaattgttc aaatatattt ttattgtaag caatcgcaaa cgaattcacc
    10381 ccgttagaca gtccaactaa ttgcccgtcg taagtcgtcg ttgctaaata ggattcatct
    10441 gcattagaaa tatcaattat cccttgctcc acatattcat ctaatggttc aatctgattg
    10501 atgtattgag gaaattgatt gttcagttgg aaaatgtccc agatatcatc tgatgctgct
    10561 agtgtaccta attttgtgta atatccttca taatcataaa attcataatc gatcgtcaca
    10621 tggggatttt tttcttcata aagctcgatt acttttactg ttgcatcgtg tcggacttgc
    10681 gaaccccacc aagccatgtt cagcgtgatt tttttgtcat cttcttgccc acaagccgtc
    10741 aagaagaata acataacact taaagctaag atgattctca ttcttttctt cattctctgc
    10801 acctctactt ccattgttat ttttcaatat actgtatttt tatacagaac atatttatta
    10861 aacgatcttt ttcaagggga ataaaaacca agacttgcct tatcgttctg cctaggatga
    10921 acaactcaat actttcaaga aaaattagat agtgctgatt cttctttcat cgctaaaaac
    10981 acttcttgct tcaatgatgg gaggataatt acgcaaagaa aaaaggatat cagacaactg
    11041 cccaatatcc ttgacatagc taaataagca tgcttcttcg tgtgcgctct tccctaaaaa
    11101 acagaaaaat aactagtaat tctgtatgaa attaggtaca atatacacgt cgatgcacaa
    11161 gcattgacta tggcaacttg attggtcagt tcccgctgac ttttcaagcc ccgacaattt
    11221 agctgctact aaattgtcgg gtgccttttt tagtttttat tcgtttttca ttctatagaa
    11281 agcgcttcaa taaatcaaga catttttttg ttagaatgct aataataaca aaaggtttta
    11341 actcttctta tgtacattac aggtaaaaaa acaaaaaaat ttctcttttt tttgaattct
    11401 gaacaaatag tactggagta caaaagacga ggaagagcca ctatctgctc ttcctcgtct
    11461 tttgattctc acctgttatt catgattctt tttctcagtt tcccgatatt tttccatttt
    11521 acgactaaaa agctctccgc tacttggtgg cgtatatgtg atggcatttg ctcctgcttg
    11581 aatggtctca gcaatacttt cttccgttgg accacctgtc gcaatgatag gaagttcagg
    11641 atatttttta cgaaaatggc gtactgcttc tgcagtttct ttacctgcac tgatattgat
    11701 aatcgaaata ccagctgcta acttttcatc tatttctgta tatttcgacg ttaccgtact
    11761 gataactgga atatcaacta ctcgacaaac ttcttttact gtctctaaag gcgtaggacc
    11821 gttcaaaacg acccctaacg aaccttgcgc ttctgcgaac atgctcatgt aggaagaacg
    11881 agcaccttgt gtcaaaccac cacctacacc ggagaaaact ggaatgtcag ctgcctcaat
    11941 aatgctttta gtaatcgctg gatgaggtgt aaaaggatac acagcaatta ctgcattcgc
    12001 atttgtatta cgtatgatcg caatatccgt agtaaagatg atcgaacgga tcaattttcc
    12061 aaatatcttg atcccgcttg cttctaaaat gatttccggt actcttacga tatcttttct
    12121 caattccgtc atgatttctg gcgtaaattg ttctgccatc agccacttct cctttgctat
    12181 ctccggtcat actgattcaa ttgatacgtc tcgatgacct ggatgatttt ttgcgcataa
    12241 ccggggtctg tcgcataacc tgcatcttgc aatgcttgtg cggcttcctg atagttggtg
    12301 gccgtaatca ctttttcata cagcgcagga ttccaatcta ctccttggac gaacagttgt
    12361 gtatggtcat ccatggactg ttcccatgaa tcatacactt tgaagtctcc ttggatagtt
    12421 atccattctt cgttaacaaa ttcctttgtt tccaggctta ctttcttctg atccccataa
    12481 gccttgatcc cgaaaagatt attgtatttg ctagccaagg tactttttcc ccaatttgat
    12541 tctaatatag cttgtcctaa aatgatacta ggaagcactc cataaccatc ttgtagttct
    12601 tgggcatgcg gaacaagccg ttcaataaat tgctgatggg attgcgcttc attttcttgc
    12661 tgattatcag taaaaggaga agacagattc ttcaaggaaa aaacaaaggc taagagtatc
    12721 actgctacac cggcaaccac tgctggccaa cggatattct tgcgcttttg ctttttccac
    12781 ttttttttcg ccatgatcaa ttcctacttt tcatttaatt cattcagata ttcttctagc
    12841 gtcaattggt gtttggtgat atactcagcg ttttctttac cgacataacg taagtgccaa
    12901 ggttcatatg tgatcttagt tatatcttcg tgtccttttg gataacgaac gataaatcca
    12961 tactgcgcag cattatctgc gatccattta gctccaggct gatctccgta acttgcatcc
    13021 aatacagtag aaggataact gttgtaccag ttttgatcga cgacatcgac tgccagacct
    13081 gtgtggtgtt cgctgtatcc tggctccgta atcgtttctt tagtaatctt tgtcgcctcc
    13141 tcttctgata caccttgacg actcatcaca tcttggacat tttgttcgaa aacttgttgt
    13201 tgggatgata cagaccggta agctgagacc atcactaatg gaaatccagc tgcttcagca
    13261 gccttggcaa atgcttcata ttctggagca atgcgtttgt cgatcaagta tccgttactt
    13321 aatgatgcca attgagtaga ctcatcaatt tctttttcta atttgtgatc tggaccaacc
    13381 aaaactagct tccaatcgtc tgttttaaca tctggcaatt gtttggacgt acgatctttt
    13441 gtttctttgt cctttacctt gctgctggtt gtttgactgt tcttttcttt actagcagtt
    13501 gttttggtgc ttgttcgttc gactgcttga ttgccggctt gaacatgcga atcatcatcg
    13561 ctaaaaataa catagcccat tgctgcgatc ataatcacaa ctgctccaaa tcctaaaaat
    13621 ttattcactt gaaaaatcct ttcgttgttc ttcacgttcc ccattcgcct catgaacgta
    13681 agtttcttcc agattttcat tcacccaatc gagaagttcg attttttctc cttcgatctg
    13741 tttttgaaca tctgctggca gacgaaaatt caaaatatca tcatagcccc attcatcata
    13801 ttgaatactt actttcaagt caaggtatct accagcctgc tgattatttt tcaaagggat
    13861 cagctcgact tttgctgctt tgctgctaag ccatttttgc gcgatgcgtg ttttgatttg
    13921 cttgtactct gcaacggcaa cgtttcgttt ttcgggaaaa ataatcgttt gacgcaaaat
    13981 cttttgcgtt agcatccatt cataatcagt gaaaagattt tttactttct gcatcgcttc
    14041 agatggcaga tttttctcgc catctttcca tttttgccat tgttctggac tgatcagcaa
    14101 gtattcatct aaaaaatttt tttcagtttc aaactgttct aataatacac tcataataag
    14161 atcaatatat acttgatgca cgtttatccc ctccttttaa ctattctact taagttttga
    14221 tcaaaaagca cctcttaaat cgctgctttc atttttttta atgtttcaag tcctctcctc
    14281 tttattcttc gtgatttttg ttataataat agacagacaa gaaactcaat ccaactatga
    14341 aggagcgttt tcagatgaaa aaatatcaat ggggaatcgt tggtcttggt accatcgcac
    14401 atgagtttgc cgaaagcttc aatcaagaga cgagcgaact aacagcagtt gcttccagaa
    14461 cttcagaaaa agcggaaaac ttcgctcacc gttacaatat tccaaaagct tacggaagct
    14521 atcaagaaat gttagacgat gctgaaatcg acatcgtgta cattgctgta ccaaatagac
    14581 agcatatcga ccatatcttg gcagcattgg aagcaggcaa acacgttcta tgtgaaaaag
    14641 ccatcacgat gaataaaaaa gaactggctg acgctatgcg cttagcagaa gaaaagaatt
    14701 tgatcttagc agaagcgatg acaatcttca acatgccact ttatcagcag ttacgctcaa
    14761 taatggatac aggaaaactg ggggctttaa aaatgatcca agcacctttc ggcagttaca
    14821 aagaaccaga ccctaaaaac cggttcttca atccagaatt agctggagga gctctgttgg
    14881 atatcggtac gtatgctgta tctttcgctc gtttcttttt gagctctcaa cctgaagtag
    14941 ttgcctctac aatggttcct tttgaaacag gcgtggacga acaatccgtc actatcttgc
    15001 gtaacaaaga aaatgaactt gctgctgtca gcttgacttt tcaggcaaaa atgccaaaag
    15061 tcggtatcgt tgcctttgag aacggttatg tgacgattgc tgattacccg cgtgctgatc
    15121 gtgcggagat cttatttaca gatggtacaa aggaattcat cgaaagcggg aatacatctg
    15181 aagcaatgaa ttacgaaata aagaatatgg tacacatgat tgaaggtgac ttgcccaatc
    15241 gctctctctt cttgacaaaa gatgtcatcg aaatcttgga tcaaatgcaa gttctatgga
    15301 aaaatatgta attttaaaag gttgtgaaaa agctgttctg catcaagtaa taagaacagc
    15361 caaacaaaaa tagctcctcc tatttttcgt ttggctggac ttattaccgc agaggaaagc
    15421 ttttgaacac cgtttaagaa agaggttgtg agcctaccgt tcagctccga gtcacaagga
    15481 gcaatttcaa aaaacagctt ctcatgtttg ttgaatattg tgacttgtga ccgaggagtt
    15541 gacaggagaa caccgtttaa agaagaggtt gtgaaatcag ctttttgacc tgaatattag
    15601 ctgaattcac aacctctatt tttggatttt ttgttgggag aattctgcct tgatttcaat
    15661 aaaactttta aaagactgca aaaactggaa caattctgct cgatcctcga attcagggcg
    15721 actagtcggc agaggcattt gccgatacaa tacataaact tcttcaatac gtgttaaaat
    15781 ttgtctgcca tcatttgact ctgaaaatgt ttctgcagta taaatcaata aaccatagat
    15841 atgttttcca taataagctg gttgctggat tctctctaga ttttccagca tgtctttaat
    15901 cacattggcc tgtgctcttc tcatagagaa gtaagtttcg tagtatagcg gctgtctgag
    15961 ccagtagttt tcttgatgtt ctctggcatc ttcttggctt tcgcgaatga atgtcagtaa
    16021 atgttcgcat ttgatctgca catcttcttt attttctgat aaaagaaatt ctgccatttg
    16081 tctcaaaata tttcgaaatt ctttttcgat ctgtatttgg ttttccttca gctgcttcgt
    16141 acgatctggc atgtaaacat ttgcaagcaa agcaaatact aatccaatcg ccattaatcc
    16201 cgcttcattg ccgatcagct gccaagacat gttttcctcc actaaataat gagtgaccaa
    16261 aaccgaattt actactatcc cttcggttaa tttgaagcga gaggcaagcg ggatgaacag
    16321 gaataaaaaa agactgaagc caaaaatagt atacccaaca gcaaaaaaac tgaacagtgc
    16381 tgttgccgta gctaatataa aggctgcgat tcgattcatg cccgtacgca atgtagagcg
    16441 cttggtattt cctacactta ataatgcaat gatccctgca gctggccaat acaacagcga
    16501 taagctaccc gctgtgacca tcgaaaagaa gacactgatc actgttttga ctgttcgcat
    16561 tcctatacgc atttacttcc ccctttttta ttttaattat acttgaataa tgaaaaacta
    16621 gctatcagac aaaagtttag gtatgatagt aaaaagacca aaaaggagaa tctctatgca
    16681 atttggccgt tttcgactag gtatgcgtac gatcaaaagt gccttagctg tttttttatg
    16741 tatcttgttt tttcatttga ctcaacgagg aatcccgatg attgctgcat tatctgctgt
    16801 tttttctttg cgtcaggact tgactacaac cgtttctttt ggaaagtcta ggatcatcgg
    16861 caatacatta ggtggtacat tagctatcat ttattttttt gtcaaaagct attttcacaa
    16921 tgattttttg gtagaattga tcttgcttcc tattctagtt attattgtga tcgtcgtgtc
    16981 agatggtatt aacaataatt ccggtatcat ttccgcaata gctacgttgt tgctgattgc
    17041 gttgagcatc cctcaaggcg aatccgcttt gtatgctatc caacgtgtac tagatacttt
    17101 tatagggaca ttcattgcaa tcggtatcaa tttctttctt cgtcctcctg aaacagaaaa
    17161 gaaggaagaa ctatcagaag atttggtcga actccaaaag aaagaacgat tgctaagaac
    17221 aaatttacaa gagatagaag ctaagatcca aaaacaaaag aaaaatagcg aggactaatt
    17281 ttcttttgca ataaaataaa aaaatggaga gaagaacttt aaaaagtctt cctccatttt
    17341 tgattgaacg gtgaagtgta ctactttatt cttccttcat cgagttttgt tctgcctctt
    17401 ttggcgcttc atacatattt acttggctag tagtcccacg tgtcgacaaa ttctctacga
    17461 tttccttcaa gtcaagtcct gttgtcgctt ttagtgtttc ttgagtagaa gaaagcaaat
    17521 ttgtggcata atttgtcact cggttcgctc cactggtttc tcctgaactg cttcctgtat
    17581 cgacaacaga gattttttcg atatttccta gcggctgtgc tgcttcgcgc atcaattgcg
    17641 gtagcatatc gataaccata ctcaagacag ctgcttcgcc atattccttg aaggcattgg
    17701 cgatcttttg ttttgcttcg gcttctgctt ttccttttgc tagtgctgct tcggcttctg
    17761 cttgtccagc taaacgtgtc ttattggctt ccgcttctgc cagtgcttcc acacggaatc
    17821 gttctgcttc ggcttcagtc acttctttga ctttttgagc ttgtgcttct tgttctttag
    17881 cgtaccgatc ggcatctgct tttttcttca cttccgaatc atattgcttt tcacgacgtg
    17941 tgatttcttt ttcttccagt tcgatttgtt tttggcgttc tacgatttta atctgcattt
    18001 cctgttcgat tacttgttgt tgtgctcggg cactttctaa attgtaggct tggtctgctt
    18061 ttgctttagc aacatcttgt tcttgtttgt aggttgctaa tttcagttct ttttctttta
    18121 atgattctgc gatctctgtt tgtcgctgta attcagctgc ttgtgattct ttttctgctt
    18181 gagctttttt gatacgcgtt tcttttagag cttccgcttc tgctatatct gcatctcttt
    18241 tgacttgggc gattcttggt ttccctaaag agtcaagata accgtttttg tctctgacct
    18301 ctttgatagt gaaagaaacg atgatcaacc ccatttttgc aaggtctaca gaagcgactt
    18361 cttgtacgct ttgactgaac ttatcgcgat tttgatagat ttcttctaca gtcatagaac
    18421 caagaatcga gcgcaagtgt ccttctaata cttctcttgc ttcattttcc agttcttctc
    18481 ttgtcttgcc taaaaactgt tctgctgctg tagcaatctc ttctacagat gaaccgattt
    18541 tgatgatcga agtaccatca cacatcaccg gtacgccttg ttccgtatac acttcagggg
    18601 tggagacgtc caatttactc gataatagac tgatccgatt cgaacgttga aaaactggca
    18661 agacgaatgc gccacctcca cgcacaattt tgattttgtt attgctttca tcaatgtgca
    18721 catttttgct tcctaaataa cttccactga tgatcaacgc ttcatccggt tttgctgtct
    18781 ggtacttcgc tacaaagacg atcagcaaca tcagtaaaat caaagctgcg attgcgatcg
    18841 cgataaaaac aactggtatt tccatttaac acacgctcct ttaagctttt atcatgcttt
    18901 cataaggcat gacataacag attctttcct tgatctcaat gatcaatact ttcgttcccc
    18961 gctcagctgt ctgttcttgg gggctgtaaa aacaagctgg acgactaatg gaaccggtaa
    19021 cacttttcaa ctgaatttct cccatacctc gtacagggat cggtgtgatc acaacagcta
    19081 cttgtccttc cagagttttt tcagaagacg acatcgttga ttcggcatgt ttcatcggca
    19141 tcaatatata gaaattaagc aaaaatacca acactatcga tatgcctcca ctagcaataa
    19201 ataaaaataa agatgattga tctgttaaac gctcacctag aaaaccaaat aagcttgtga
    19261 aagttaacca agggataata agcatcggat caagcgggcc atcaaaatcg aatacatctc
    19321 caaaaataat caataggact gccaatcctg cacaaatcag caatgtatac caataaatcg
    19381 tctcaagtgc gattccccct atcatataca cgcctccttc catttctacg tctatcatac
    19441 caatgtttga aaattctgta tagcttcttt taccgactca gtggtgtttt ttataggaag
    19501 gttgtgttta tcgttgacaa tttgttaaat ttttgttttc atgttaaaat tttcacaata
    19561 aattctttcg aggtagccta tgtttccttt ttttaagaaa aaaccattaa agctccagaa
    19621 cacgatcatc cttgtcgttt gtttctccac attcgtttct ttgattgttt ctgccatatt
    19681 gatcaggaat tttattcttt cgatgaaccg atctagtgtg tcagataaaa tctctagcat
    19741 tgctaaaatc gttgccaatg ataaactcat ccaaaaaaag ctgcagactg gtgacccgac
    19801 acatgcgatt caaggaataa caaatgaaat catgattgca tcgaatttag attttatcgt
    19861 tgtcatggac cctgaaggca ttcgatattc tcacgcagac ccaagttaca tcggtcttcc
    19921 tttctcaagt catgaagatg tgaaaaaagt gattggagga caagaatttt tctctgaaaa
    19981 agaaggaatg ttagggttcg gcttccgcta tttcgtccct atttattatg atcaagagat
    20041 tattggtgca gtttgtgttg gcttaacaaa caacacgatt gacaatcaag tcaaagatgg
    20101 acaaaaaatg attgtttttt cattgattat tggacttagc gtaggcatca taggcgccgt
    20161 ttttcttgcc caaaaaatga aaaaagtact gcttggactt gaaccacaag aaatagcaga
    20221 acagttaagt gaaaaaacga ttatcgaaaa tgaagtagat gaaggaatct tagccatctc
    20281 ttctgataaa actatcctct tgatcaatac acctgcaaaa aacattttct accaaacaga
    20341 gcagctattg gatttcgaag taggaaaaaa atcagtgata atctatatga cgttttgttt
    20401 aaagagacga ttgagcatca aacgaccgtt cggactcaat ccatgtattt aaatgggata
    20461 gaaactattt ctagcgttac acctcttttt agcaatgacc gttttttagg agcagtcgca
    20521 actttacgaa atcgatcaga agtaatgaaa ttaatggagg agcttggggg gacgaaacaa
    20581 tatataaact ccatgagagc tcaaacccat gaattcatga ataaacttca tgtcatttct
    20641 ggtttgattg atttgaagaa gtatgaagaa gtcacccatt ttatccagca gctaaatcaa
    20701 aattataaaa aagagattgg ccaagtctca agtctgatca aagtacctgc aattgccggt
    20761 tttttattag gcaaaataaa cgaagccaaa gaacaatctg ttgaaatgct gttagaaagc
    20821 gattctttta tccctaaaat ggagatggat gactccatcc attcactttt gcaagtgtta
    20881 gggaacttac ttgataatgc aaaagaagcc gtgctacaaa aacatgaaag aaacggacag
    20941 gtccggattc atttaaacta tgacacagaa gctgaagtat tcatcgtgac cgtctgcgat
    21001 gacggtgtcg ggatatcaga agaaatctca gaaaaaatat ttacgctagg tttttcgacg
    21061 aagggaatca atcgtgggtt tggattgaat ctcatccatt caatcgtcaa acgccacggt
    21121 ggatttttag aattttccaa acaaacaaat ggcggtacaa tcgtctatct agaattacca
    21181 aaaaaggaag atcaaaatga tgacaaatgt cctgataatc gaagatgatc caatggttgc
    21241 agaattgaat cgtacttttg tggaaagcat ccctaatatg aacgtaatcg aaagtttcag
    21301 aaatgtagaa gaaggaaaaa aatatttcag acaaaaaaac gcagatctgc tgctgcttga
    21361 tgtatacatg cctggcgaaa gtggtatcca ttttttatct tggcttagac agcaaggaaa
    21421 tgatatccca gtgattttga tcactgctgc agatgatatc gaaaccataa agaatgtgat
    21481 ccgctacggt gccttagatt atttgatcaa accgttcact ttgcaacgtt ttcagctggc
    21541 gatcgaaaag tatcttgctt tctctcagac aatccaaacg actgaaaaag ccgatcagaa
    21601 attgatcgat caagtattca accacaccac tcccattcac gaaataccat cattcatcga
    21661 tttgcctaaa ggtctgtcaa aactgacttt aaaaaaaata agtgcattta ttctaaaaga
    21721 agatactatt ttttcaacaa aagaactggc tcaaaaaagc tcactgtcac gtatatccgt
    21781 caagaaatat ttgaattttt tagtagaaca agatatattg aacgaaagaa tcaaatattt
    21841 ggaaatcggc cggccgctaa cctgttatga attgacaaag aatgggcgga aaaagctcac
    21901 agattactgt tgagtcatcg aattatccca tacaaaatag tccaagtata cagaagtaac
    21961 aactgtttac ttggactatt tttaaacatt ttattgacga atatccatgt tttcttatct
    22021 taattgacgg gctttaggaa gagatacctc atttttcgtt tttccagttt ctattatttc
    22081 acgaaagttc tcgaaacttg tcgaaatcat atttgataat gcctcatctg tatttgaacc
    22141 tacatgcggg gttacaagaa ctctagggta catttctact aattgctgga tagcagggtc
    22201 tggaatcgtc tcccatggct tgaaggatcg gaaaaaaacc tctgtttcat ttgcaaaaac
    22261 atcggttgca aaaccagcta aatgattgct tttcaaagct cttaaaatag cttcattatc
    22321 ttgaagctct ccacgggctg tattaatcaa gatgctacct tttttcattt gagctaaaaa
    22381 tccttcattg atcatttgat cgttttcacc tgggatatga ggtacatgaa tactgacaat
    22441 atcgcttttg gctaataact cttcaagcga gtggaacgtc aatacttttt tagcagcttc
    22501 tgtctgatga atatcgtagc cgatcacttt ggctcctagt cccttaaaca aaacagcttc
    22561 cgtcaaccct atcctgcctg ttcctatgat ccctactgtg caatttctga tttcacgact
    22621 aaacatttgc tcatccactc gaaaatcttt gaaagatgtt cggatcgtcg tatacgcagt
    22681 atttctcaaa agcatcatgg caaaagtcaa agataactct gctatcgcat ttggcgaata
    22741 cgatggtaca cgggctactg tcataccaaa atcagctgcc gcttgtagat cgatatgatt
    22801 gactcccaca gtccgtgtaa aaacataacg aatcccatat tcctgcattt ttgcaagatt
    22861 ggtctcatct gctatacaat ttcccctcaa caatacagca tcatggtcct ttgctgacac
    22921 gatattgtca tgtgtcaaca gttcttcata aagactgaga tcataatggt aaatattcaa
    22981 tttttcaaaa aaatctatct cgttcggacg aacaccgtaa catgcgattc tcatactttc
    23041 ttcctccatt tcttatgaaa ataaacctat gccatgaatc atttgtaata caacgatcca
    23101 tacaggtgtc agtaccacag caaaaatagt tgacagtaag gatgcattgg aggctaaaag
    23161 tgcctcccta tcaaaactga tagcataggc tgctgccact gttgcagctg gtgtagccat
    23221 catgataacg attgttgcca gtgcttcaaa attgactggt agaatattcg ttgccgtcaa
    23281 aactgctaga atcacgatat tcaatgcagg tacaaatacc actttgacta gactgtaata
    23341 ccatgaagtt ttttcagatg cagctttttt gaagcttact tcacctaatg ttgcgccgat
    23401 tgctagccac gccaatggtg aacttaaacc agctaaataa tccatcggct gaaatagcca
    23461 cggtgcagtc tggtcaattc gcaaaaacgc tacttggctt atctctcctt ctgcacctgt
    23521 aactgacact tgtggcagat acccttggaa cagccaaatg aaaaggccgg caaaagtagc
    23581 gataatgatt ggattgacga acatcgcttt gatattttta gcttccatct tcaagccact
    23641 catttttatg tatccataag aatacaaaaa gattcggtag ccaatattga aaattgagga
    23701 atacatcacg ccgacaggac cataaatcgc actgacgata ggcgttccga aaaaggtcgt
    23761 agaaccaaag atcgtcagga cacgcaacgt tgtttgctta tcccctttat agcttaagaa
    23821 aaaaggtttt gaaactagaa taagaataat ataaataact aatccccaaa tcaagacatt
    23881 gattccttgc atcagtaatt ctgtattgat atcttgcatg aaagctcgaa atgccagtgc
    23941 tggcaaagcc actgttaaga cgactttcga tagaattttt ccaacagatt cagaaaaaat
    24001 cccctttttt ctacaaacaa accctaataa tataataaaa actgttgaag tgattgcact
    24061 gacaatcgac atatctgtca aagtcgatcg aaaaatgtca aataagttca tctttctcct
    24121 cctaattatg ttcatttatt cacaataaaa aaaagctttt attttgctaa tacgtattaa
    24181 catttttaat tttatcttta cattgttact tattgaacaa taggaaatta aagaaacttt
    24241 tgaaacttat gtaaagtgat ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa
    24301 gaagtttagt tttaaggtta acttaaatta tattatgcgt ggaaacgttc cacgccatct
    24361 agataagttg cttccaatgt catatcaggg ttcaatacga taaagtctgc atcgcgtcct
    24421 tgagcgattt ttccacatgt atcatcgatt ttacagctga ttgctggaat cagactagcc
    24481 atcatgactg cttgttctgg tgttgctaat ccccagtcta caacattttt aattgcttct
    24541 ttcaatttca atatacttcc agctaagttg ccagattcta gacgtgctgt tccttctttt
    24601 actactactg gaaattcgcc taaattgtaa tttccatcag gcatcccacc ggccatcata
    24661 cagtctgtaa ttaatgcgac atgatcatgt cctgcttttt ccattaggat tcccgcagca
    24721 tttggatgta catgatggcc atcacaaatc aattcagaaa agacatgttc taaagataat
    24781 aacgcaccca ccatacctgg ttcgcgatga ttcaatccac gcataccatt gaaagcatgg
    24841 acaaatacac ttgcgcctgc ttcaacagct ttttgcgctt cttctagtgt agcattactg
    24901 tgtcctagag aaacaactac tccttcttca gtcacttgtt tgacaaactc ttctacacct
    24961 tgacgttccg gtgctaaagc aatttttttg attaaaccac ctgaagcttc ttgccattca
    25021 tggaaagtat ccaaatcagg atccccgaaa taacttgggt tttgtgcccc tttgtgttct
    25081 tctgtgaaga aaggaccttc aaaatagatt cctttgattt tagctcctgg tgcttcctga
    25141 tacattttgc cgattgtctc agctacatct tttaaacgtt ctttgcttga cgtcaatgtt
    25201 gttggtaaaa atgaagtcac tccacaagat aacagacctt cagacatcac tttgatgcct
    25261 tctgcatcat tatccattac atcatgattc atatagccgt gaatatgtgt atctaccaaa
    25321 ccaggagcaa tccatttgcc gctttggtca atgatgttga cttctccctc aggtagttct
    25381 ttggtgtatt caccaaaaag tccatcggta atttcaagat agcctgcatg tttgacacct
    25441 gacgctaaaa agaatttgtc agcatagata aaagttctca ttctattctc tccctttgtt
    25501 tttctacctc taaagtactc ttatttagag aagaagtcaa gaatggtcta caccatttac
    25561 aaattttttg aaatttgttt cacagcttgt gctaagcctt catagaatgc tgctctttct
    25621 ggttcttctt tacgcttgta tattaagctt ttaagcacaa ataaatcatt gaggtaatag
    25681 tagctattct tctcttgagc ccaatagatt ccttgattca cctgttctaa cgcttcgtcg
    25741 atttcatttt gatggaataa gaaatttcca taattataaa aaatttgcga aaattcactt
    25801 tttgatcgtt catcagctga ttgatgcagc agttggatgc ttcgactcaa atagtagcgt
    25861 gcttcagccg tttttcctgc cacaccatat atttttccga tacaactaat cagttgtatt
    25921 tctgtgtcag aaacatggat tctttctttt tggctagagt agcttaatgc ttctttcaaa
    25981 taataaaggg actgagagat attttgacgc aacgtgaatt cgcaaacacc gagataataa
    26041 taatagcgtt gaaagtcagt tgcttgatac agattttcag aaatatcaga agattttagt
    26101 acttgttcta acttccgata atcacgtttt tggaaacaac tatccaacag ttcaaataat
    26161 ttatcgtttg tccgagtagt ttttcgagag agattcatca catgatcaat cgttacccct
    26221 agccgttgac aaatctgcat catcaccaat acattcggca gttcctcatt attttcgatc
    26281 cgacttaata cactttgtga acaaatatca tgcgaaagca ttttttgcgt cattttttgt
    26341 tcttttcgaa tctctttaat gatcgttcca aaggactcga ttaacgtttc cataagtatc
    26401 ctccccaaaa tatgcgtatt cgcattctat tcgtatttta ctttaggaag tcgcaaactt
    26461 caagaaaaga cttgcaacta aataatatac tttagttatt taagaaaact ccactagaaa
    26521 gagagcctca gtgtgtttgt ctgtggccct cctactgaaa aatttggttt ttaaaatcat
    26581 ttttggcgat aggttgctag gaattctctc attttcttag ctgtttgttt caaatcatcc
    26641 attttaggaa gataaacgat tcgaaaatga tctggctggt tccaattaaa tccaccacca
    26701 tgtacaagta gaatatgatg ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa
    26761 atattgaatc gctttgtatc aatttttggg aatatataaa aagcagcttt tggttttacg
    26821 gctgaaagac caggaatatc attgattgca ttatagatat attctctttg ttcatagatc
    26881 cttccgcctg gcaggagcag ctcatcgaca ctttgataac cgcctagcgc ggtttggata
    26941 atttgctgag acaatacatt ggaacacaaa cgcatggagg acaacatatt cagcccttct
    27001 atatagcctt tgacgtgtga cttatctcca cttaggacca tccagcctac tcgaaatcct
    27061 gctacacgat gagatttgga tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat
    27121 gtagcaatcg gaatatgttc tagaccatcc atcactaagc gatcatagat ttcgtcggaa
    27181 taaataatca ggttattttg tcgagctact tcaacgattt cttctaataa ttcttttgga
    27241 taaagtgccc cagtaggatt attaggatta atgataacaa tagcttttgt ccgactcgtt
    27301 atttttgatt tgatatcatc aatatcagga taccattctg cctcttcatc gcaaatataa
    27361 tgaacaggtg tgcctccagc taatgcaata gaagccgtcc acaacggata gtccggcatt
    27421 ggaaccagta cttcatctcc attgtccagc aacccctgca tacacatcgt gatcaattcg
    27481 ctcactccgt tgccggtgta gatatcattg atcgtcacat tcggaaaatg tttcagttgg
    27541 caatattgct caattgcttt tctagcagaa aagatcccct tggaatctga gtagccttct
    27601 gagtcacggg cattcatgat caaatcacga atgacttcgt ttggtgcttc aaatccaaaa
    27661 ggtgcgggat ttcccgtgtt caattttaaa atactaatgc cctcttcttg catccgctcc
    27721 gcttcttcta gaacgggacc gcgaacatcg taactgacac cttctaattt gttggatttt
    27781 tcaaattctc tcatcactta gccaatccct catttcaaaa agttagaact ttccaaattt
    27841 tttgttattt actatttcaa tatcatactc catcctagaa aattttgcca cttatttagg
    27901 aaaaacgtaa gtattaaaaa aacacaaaca agcgttgaga acttaattgt catgatagtc
    27961 attccataga ttcgctgccc aatccattgc aattccacaa atgattccta gcataaaacc
    28021 aagcaaaaaa cgtccgaatg caagtccaat caatacacct aagatcattc ccagtcctga
    28081 aaattctccg tcatatttct ccatcttaat aagtcctccc atttttgagc ttgacaacta
    28141 tgtaaaacct cgctatcatt agtgtgttat tattacgcag atgtgttgga attggcagac
    28201 aagcatgatt gagggtcatg tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca
    28261 ctacaactcg tgaagttaat tttgcttcac gagttttttt gtttgaacgt ttctttatgc
    28321 cattatatac atatcactgt atcctttaca aaaagaacgt acaatcatgt aaacagcctg
    28381 aggtgagtat atgaaggtcg gaaacaagct actcgtagaa acttgcaagt gttaaaacaa
    28441 ttaatcgaaa aacgagtaaa atccctcacc taatactaaa gcgagggatt ttttgtatat
    28501 tatctgttta tcaccgttat tataaaggta caaataccaa gcacggttaa tccaaattgc
    28561 ataaattcat ttttttgtac actcatgctt ttcattagct ctttaactgc cattagtact
    28621 aagatggcga taacaacata aagtatatag tacgtccgca ttccggaagc ccatactgtg
    28681 ggagaaaatc ccataattaa ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa
    28741 atagaagttt tcttattgtc aaaacttaaa taaattccaa caattaaaca tagtaataat
    28801 gccaagacta ctaaagtagc tatccaagta cctggataaa aaagtgacag tttcgttcct
    28861 aactctgtaa agagattatt caggttacta ctactccaaa tcattgccct tttatttcca
    28921 ttgacgtaag taaagctttg acccattgta ttaccaaaat aaataatcag gtttaagaaa
    28981 aacggtaatg cagtcaatat acgcacataa tagttttgac atttcctata tgttagaagg
    29041 aaaattaaga aaaatagcaa taaaaataag atattcatgt ctaaaaaaaa cggctttcca
    29101 aatgatgaaa tacctaaatc taacttatta agaaagttga aatttttata ttcaggaaac
    29161 catttattaa tttcatgtgc agcacgtaat gcattccctg gagttgtcaa agagaaaatt
    29221 aattctgcta agcttattat tgaaaatact gataacttat aattatatcg tccattgaca
    29281 atcaaatata aactaactat acttgttaga acaaaaaaac aggcgttgac ttgttcttga
    29341 ttagttaaaa atatcaataa aggaatactt acccaatata tttttctatt tatttcttct
    29401 cctcttaaga gttgaaaaaa cggataaaaa gttagtaaac taaatgctac tggccactga
    29461 tagttcaagg tggttgctct ccaaccggtt tcccccatta ctgtcaatgg tattaaacag
    29521 tatatagagc aaaccaataa taaattttta acatttattt tctcaaaaac a
//
LOCUS       DAZUMM010000028        27837 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000028 DAZUMM010000000
VERSION     DAZUMM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.028"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            306..1565
                     /gene="lysA"
                     /locus_tag="ACG8E6_001203"
     CDS             306..1565
                     /gene="lysA"
                     /locus_tag="ACG8E6_001203"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFS5061634.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1552..2352
                     /gene="dapF"
                     /locus_tag="ACG8E6_001204"
     CDS             1552..2352
                     /gene="dapF"
                     /locus_tag="ACG8E6_001204"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFS5061635.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2355..3236
                     /gene="dapA"
                     /locus_tag="ACG8E6_001205"
     CDS             2355..3236
                     /gene="dapA"
                     /locus_tag="ACG8E6_001205"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFS5061636.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3233..3949
                     /gene="dapB"
                     /locus_tag="ACG8E6_001206"
     CDS             3233..3949
                     /gene="dapB"
                     /locus_tag="ACG8E6_001206"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFS5061637.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3955..4650
                     /gene="dapD"
                     /locus_tag="ACG8E6_001207"
     CDS             3955..4650
                     /gene="dapD"
                     /locus_tag="ACG8E6_001207"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFS5061638.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4666..5667
                     /locus_tag="ACG8E6_001208"
     CDS             4666..5667
                     /locus_tag="ACG8E6_001208"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFS5061639.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5664..7025
                     /locus_tag="ACG8E6_001209"
     CDS             5664..7025
                     /locus_tag="ACG8E6_001209"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFS5061640.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6985..8136
                     /locus_tag="ACG8E6_001210"
     CDS             6985..8136
                     /locus_tag="ACG8E6_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS5061641.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            8111..9280
                     /locus_tag="ACG8E6_001211"
     CDS             8111..9280
                     /locus_tag="ACG8E6_001211"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS5061642.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9455..10834
                     /locus_tag="ACG8E6_001212"
     CDS             9455..10834
                     /locus_tag="ACG8E6_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5061643.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            11115..11912
                     /locus_tag="ACG8E6_001213"
                     /pseudo
     CDS             11115..11912
                     /locus_tag="ACG8E6_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            12101..12769
                     /locus_tag="ACG8E6_001214"
     CDS             12101..12769
                     /locus_tag="ACG8E6_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFS5061644.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(12969..13355)
                     /locus_tag="ACG8E6_001215"
     CDS             complement(12969..13355)
                     /locus_tag="ACG8E6_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061645.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(13327..14709)
                     /locus_tag="ACG8E6_001216"
     CDS             complement(13327..14709)
                     /locus_tag="ACG8E6_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS5061646.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(14865..16052)
                     /locus_tag="ACG8E6_001217"
     CDS             complement(14865..16052)
                     /locus_tag="ACG8E6_001217"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFS5061647.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(16059..17090)
                     /locus_tag="ACG8E6_001218"
     CDS             complement(16059..17090)
                     /locus_tag="ACG8E6_001218"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS5061648.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(17106..18560)
                     /locus_tag="ACG8E6_001219"
     CDS             complement(17106..18560)
                     /locus_tag="ACG8E6_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFS5061649.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            18735..19640
                     /locus_tag="ACG8E6_001220"
     CDS             18735..19640
                     /locus_tag="ACG8E6_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFS5061650.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            19731..20408
                     /locus_tag="ACG8E6_001221"
     CDS             19731..20408
                     /locus_tag="ACG8E6_001221"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS5061651.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            20405..20575
                     /locus_tag="ACG8E6_001222"
     CDS             20405..20575
                     /locus_tag="ACG8E6_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061652.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            20689..21585
                     /locus_tag="ACG8E6_001223"
     CDS             20689..21585
                     /locus_tag="ACG8E6_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5061653.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21640..23010)
                     /locus_tag="ACG8E6_001224"
     CDS             complement(21640..23010)
                     /locus_tag="ACG8E6_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5061654.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            23248..23994
                     /locus_tag="ACG8E6_001225"
     CDS             23248..23994
                     /locus_tag="ACG8E6_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS5061655.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            23991..24830
                     /locus_tag="ACG8E6_001226"
     CDS             23991..24830
                     /locus_tag="ACG8E6_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS5061656.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            24831..25781
                     /locus_tag="ACG8E6_001227"
     CDS             24831..25781
                     /locus_tag="ACG8E6_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS5061657.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            25922..26674
                     /locus_tag="ACG8E6_001228"
     CDS             25922..26674
                     /locus_tag="ACG8E6_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061658.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            26693..>27837
                     /gene="queG"
                     /locus_tag="ACG8E6_001229"
     CDS             26693..>27837
                     /gene="queG"
                     /locus_tag="ACG8E6_001229"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFS5061659.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRK"
BASE COUNT         9142 a         5053 c         5840 g         7802 t
ORIGIN      
        1 tatttatgaa gaaattgaaa tgattcataa aattgaaaat atccgaacta ttccgaacta
       61 tattgaagcg aaaatatttt ttcatcacta aatagtgttg aatttatgtt tttatgtata
      121 aatagcttct aatagttcgg gtattttgtt tttataacca tttttattct tatttttaaa
      181 aaaatatttc gaaatatact agacaattca gaaaattttg tttaatatta ttccaataaa
      241 attcaaatct ttatcaaatg aaattccaat aaaaacggaa aaaaagtgaa aagaggaatg
      301 aaaaaatgac agaattgata attggcggag tcacagcaag cacattggca gaaaagtata
      361 agacacctct gtatgtgtat gatgaagaaa agatgagaca aacgatgctg tcattcaagc
      421 gaggctttgt atcgaaacgt tttgatacaa aagtgcttta cgcctctaaa gcattccaaa
      481 cgattgagat gctgcattta gttcgtgaat atggattcgg gctagatgtt gtcagtggtg
      541 gggaaatcta taccgctttg caagctgatt ttcctagaga tactatctac ttccatggta
      601 acaacaagac gccagaggaa ctgatctatg cacttgaaaa tgatttgttg catattgttg
      661 ccgataattt gatggaagtt gaattattag cagaattaag ccaacgctat ttaaaaaaaa
      721 tctgtatcat gcttcggttg aatgtcggag tagaagcaca tactcatgaa tatatcgtca
      781 ctgcgcatat tgattcaaaa ttcggcatga gctatgaaag taaagagtgc caacaagccc
      841 ttcagttgat aaaagaaagc agctatctag agttagaagg atttcatgct catatcgggt
      901 cacaaatttt tgatgttact gcttggttag ctgaaatcga caaattgacg aattacttaa
      961 ccgattttga ccaacctctt tccttgaatc ttggcggagg gtttgggatt cgttatacac
     1021 aaaatgatca gccgatccca gtcgaagagg cactaaaaaa actgattgaa tatacggaag
     1081 aagctttgga aaaacgaaaa ctgaccattc gtcaattact gatcgaaccg ggtagaagta
     1141 ttgttggaga agctggtaca acactataca cgattggttt catcaaaaag acaccacata
     1201 aaaaatatta ctttgtagat ggcgggatga cagataatat ccgtccagct ctgtatcagg
     1261 cagaatacga ttgtgattta gcagcaaaac tgtcggaaga aaaaacagag aaagtgacga
     1321 ttgcaggaaa aatgtgtgaa tcaggcgatg tggtaattaa agaagcttat ttgccgaaag
     1381 cagaatcggg tgatctttta gctgtttatt ccacgggagc ttatggatac tcgatgagca
     1441 gcaactacaa tcgagcgaca cgaccagcag ttgtttttgt caataatggc caatcaagac
     1501 tgattgttcg cagacaagca ttttctgatc tactaagggg ggaagtttcc tatgatcctt
     1561 cataaatata atggctgcgg aaatgatttt atcttattag attacgcaga agacacagat
     1621 tatagtcaat tagccgcttc tttatgcaca aaagaaaaat atgatataga cggattgatc
     1681 gccgtaaaaa tcgatccgtt ggaaatgatc tattacaaca aagatggcag ccgcgcgccg
     1741 atgtgcggaa atggcattcg ctgttttgct cgatacgtaa atgatcagaa catgatgaat
     1801 gaacaggaat tcaacgtaga gacattagca ggcacaagaa aaattacggt gttggatccc
     1861 tcaccttttt attgcagcgt agatatggga gtgccagatg attctccgga aaaaatcaaa
     1921 gctaatcaaa cttatccaat aaaaaaccag gaaatagaga tcaatggtga aaaagtaacg
     1981 atcactagtt tatttttagg aacgattcat accgttgttt ttgttgaaaa tgcgcgtgag
     2041 gaagtctgga gaaaaagagg tgaactgatc tgtcatcatc ctttgttcaa agaaaaaacc
     2101 aatgtcaact ttgtagaagt agtcaatgaa caagaattga tcgtccgtac ctatgaaaga
     2161 ggagtaggtt ggacacttgc ttgcggtaca ggttgctgtg cctcttatgt ggttgctaag
     2221 gaaactggga aaatttcagc agatcaggta gttgttcact tggaacaagg agacttgatg
     2281 atctctggtg aggatgcgat acaaatggct ggcccagcag ttcatgaatg gacaagaata
     2341 ttggaggaat gaagatgata aaaggttcga tcgtggcgct gatcacgccg atgaatgaag
     2401 agggatcagt agattatgct ggtttggaaa aattgatcca attccattta gatgaacaaa
     2461 cagatggatt attagtttta ggtacaacag gagaatcttc tacgctcaca caatctgagg
     2521 aggagcagat ccttcaactg acagtaaaga aagtggctgg ccgtgtgccg gtgattgcag
     2581 gagctggaac gaataatacg aaagaaacga ttgaaaaagc gaagcatttt gcttctttgg
     2641 gggcagacgc acttttggtc atcacccctt actacaacaa aacaagtgat gcaggactag
     2701 ctgctcattt tacagcaatt gcagaagcct caccgattcc gcttattttg tataatgttc
     2761 cttctagaac aggcatgtcg attccaatcc atgtattagt aaatttagca gaacatccta
     2821 atatcattgg tttgaaagaa gcctcaggag atatggctta tgtgatggat gccgctcggt
     2881 tgattggaga agaattcttc ctttacagtg gaaatgatga tttgatccta ccagtaatga
     2941 gtgtaggagg aagcggtgta ataagtgtat gggcgaatat ccaacctaaa atagtccatg
     3001 aattagtaaa agatacgcag gacggaagat ggcagcaagc aaaagaaaag caattaaatg
     3061 cacttgaact gatccatgcc ttgttcagtg agaccaatcc tattcctgtc aaagcagcca
     3121 tgtcattatt agacttacct tccggacctc tacgcctgcc tttagtttct ctttcaaaag
     3181 agaaaaagaa acaattagct cagcttttgt taaaagaaag gacggttgaa aaatgaaaat
     3241 cggactgatt ggcagtggac agatgggaaa tagagtcgaa gaagttgcac aagaaagagg
     3301 agatacggtc cttcttttac ggacggcacc taaagaagca actacgcttg cttttacgga
     3361 actgccagaa ctgcttattg atttttctca tccagataat ttagagaaaa tcctttcgta
     3421 tagcttatca tatcaacttc cgttagtcat tggcacgaca ggttatacac aatcacagtt
     3481 ccaagaaata aaagaacatg ccaaacatgt acctgtgatg tatagtgcta atttttcgtt
     3541 cggcatcatg gtgatgaatc aactaatcaa gcaagcagct gcaatgttgc aaggctggca
     3601 gatcgaactg attgaaaaac atcatagtcg taaaaaagat gcaccatctg gtactgcaaa
     3661 tatgctgctt caaacaatcc aagaagtgca aaagctgcag cccgtataca actggcaagg
     3721 gaaaaaaaga gaagaaaacc aaattggtgt acacagtatt cgtgcaggtt ctttacctgg
     3781 tgaacatgat gtattgtttg ctgctactga tgaaatattt acgatcaaac atgaatcttt
     3841 ttctaatcga atctttgctg caggcgcagt agaagcagcg gactggttga aagatcagtc
     3901 accaggttat tacaaattag aagatttatt aatggacaaa ggggtgtaaa aaacgtggaa
     3961 gctcaagaaa tcatccgcta cattcaaacg gcatctaaaa agacgcctgt gaaagtctat
     4021 ctgaatgtaa ccgagccaat cgaatttaaa gaaagtaaag tttttggtga aggaagcagc
     4081 ttcgttgttt ttggtgacta tgagacgatt gctccggttt tagaagctga atcagaaaaa
     4141 atcattgaaa tcgaaatcga aactgctgca agatacagcg cagttccttt gctagatatt
     4201 aaaaaaatta atgctcgtat cgaaccaggt gctattatca gagatcaagt gtcaatcggc
     4261 aacaatgccg tgatcatgat gggagcgatt atcaatattg gtgcagtaat cggtgaaaac
     4321 acgatgattg atatgggtgc tgtcttaggt ggacgagcga cagtgggcaa aaactgccat
     4381 attggtgcag gagctgtctt agcaggtgtg atcgaaccag catcggccaa accggttatc
     4441 gtagaagacg gcgtactggt aggtgcgaat gcagtgattg ttgaaggtgt ccatatcgga
     4501 aaagatgcag tcgtggcagc aggagcagtg gttttagaag atgtggcagc agaaactgtt
     4561 gtcggtggaa tacctgcacg tgtattaaaa ataagtgatg atcagacaaa agaaaataca
     4621 gcattgattg ctgcgttacg cgaattatag aggagatgga gaaaaatgcg cgaaatagat
     4681 acctgtattg tgggggcaac cggattagtc ggccaaacga tgctcaaagt gttagaagaa
     4741 tatgattttc cagtaggacg attgaaatta ttagcttcaa aacgttcagc cggtaaagtt
     4801 tgtgtattca agaaaaagaa acacactgta caggaattga cagaaacttc atttgaagga
     4861 tatgatttag cgctattttc tgcaggtgct tctgtatcca aagagtttgc gccaatcgca
     4921 cgaagtcagg gactatttgt catagataat tcctcagcat ggcgggaaga ttcggatatt
     4981 ccattaatcg ttccagaggt caatctggaa gactatacat tgaatcggct gattgcaaat
     5041 ccaaattgtt caaccattca agcggtactt ccgcttaaag ctttacaaga agcctttggt
     5101 atccagcgag tcaattattc gacctatcaa gcagtttctg gttccgggca aaaaggcatt
     5161 gaagatcttc atcgtacgca aaatgacgag gaaccagttt tgtatcctta taatattagc
     5221 caaacggtga ttccggaaat cgatcagcct ttggaaaatg ggtatacaaa agaagaacag
     5281 aaaatgataa atgagacccg taagattctg catcagcctg ctttgccagt atcagcaacc
     5341 tgcgtacgtg tgccaatcga aaatggacat ggagtctctg tggcagttca actgcaaaaa
     5401 gagtttacga cggaagaaat tagagagtgt ctcagccagt ttccagggat tcaattagaa
     5461 gatgatctag cagcacataa atatccgacg agcattcttg ctagaggaac agatatggtt
     5521 tatgttggga ggatcagaaa agacacttct ttagagaacg gattgttgtt ctatacgact
     5581 gccgataaca tacgcaaagg agccgctgcg aatgccgtac agattgctgc agctttaatg
     5641 cagaaaatag cggaggtggc aagatgagta atacgctgaa agtagcgaaa ttcggcggaa
     5701 catcgatgtc tcatgaagag caattcaaaa aagtccgtac aattgttctt tcagatccca
     5761 atcgccaagt gatcgttgtt tctgctttag gcaaacgtac gtcagatgat gacaaagtaa
     5821 cagacttact ttatttgatt tatgcttacc ttcagcatca tgttgattgg acaccccttt
     5881 ggaaaaggat ttgtgatcgt ttcattcaag taagagatgt tcttgccctg cagttaccta
     5941 ttgaagatga actagcacgc atacagaaac aactggaaga cgaaaagata actgaagatt
     6001 atttagtaag tagaggtgaa tatctgactg ctcaactgat ggctgagtat cttggttacc
     6061 agtttattga cgcaaaagac ctgattttct ttgactatga tgggaaaatc gatcaggaaa
     6121 aaacagcaca ggaaatcaca aaagcattta aaagatatcc gaaaatggtt gttccagggt
     6181 tttacggggc gaaccctgca ggaaaagaaa aattattggg ccgtggggga agcgacatta
     6241 ccggcgcgat actggcgaag atgttgcaag cggaaagtta tgagaattgg acggatgttt
     6301 caggaatcat gatggctgat ccgcgaatca tcgatcatcc taaaatcatc gaggaaatct
     6361 cttttagaga actgagagag atggcttata tgggggctaa cgttcttcat gaagcagctg
     6421 ttttcccagt tcaagaggct gatatcccca tccaaataaa aaataccaat gatcctttcg
     6481 ctcaagggac aaaaatatcg aatcacgaaa tagaaaagga aaatggactg acaggaatag
     6541 ctggacgcaa ggattttctt tccatcactt tattcaaacg tcatatgtcc gatgaaatcg
     6601 ggtttatctg gaaagcaatg agcatttttg ctaaacatga gatatcaatc gaacatattc
     6661 cttcagggat cgacaatatc ggagtgatcg tctcagcaga agcgattgcc gataagttat
     6721 ttctcatcac gaaagaatta aaagaaacac ttggcgtgga agaaatcgtc gtgatcgaag
     6781 acttagcttt gatctctgtc gttgggggtc cgcataaaga attgattgga ttatctggga
     6841 aagtgttatc gatattgaac aaattagaaa ttcgcacatc gatcttatca caaggagcac
     6901 aggagttgaa tctgatcatc ggtgtaccga ataatcaata tgaaacagta gtgaagggta
     6961 tctatgaagg gatggtgaat acggatgctg aaagaatatc gaatggcact ccatcaaatt
     7021 cctgaaatcg ggtttcatga attcaagaca aaaaaatatt tatatgaaca agtaaaaaac
     7081 agcgaggccg ttatccatga aatcggcgaa acaggactac tcctttattt tgataatcag
     7141 caagaagaaa cgattgcttt tcgaacagat atcgatgcac tgccagtcaa agaagaaacg
     7201 aatctctctt ttgcttctaa gcatgaaggt tatatgcatg catgtggtca tgacggacat
     7261 atggcgatgc ttttagggtt gactgacttt ataactaaac acattgagga tcttatttat
     7321 aatgtagtac tgatctatca gccatcagaa gaaatttccg gtggcgcaca aagcgttatt
     7381 cagtcaggat tgttggataa atatgaagta caagcaatat ttggctttca tctatggcct
     7441 ggactagcta agggagaagt tttctctcga ccaggacctt tgatggcaca aagcagtgaa
     7501 acagacatta tcattaaagg ccagtctgca catattgctt ctagtgaaaa aggaatcgat
     7561 agtctggagg cagctgtacg atttttgaag aatgtttatg attttgaccg gcaattaccg
     7621 gcagagtgga atcatctaat gaaatttggc caaatccaag gtggaacgat ccgaaatgtt
     7681 ttagctggag aagttattgt atccggaagt atccgttctt acagttcaga tatgcaaaat
     7741 tatttgaaga ggggattaga ccatttagca gaacagtttc actctacagc tactatttct
     7801 tttcgctata atgacggcta tccagctgta atcaaccata gaggcttata tgcagcactg
     7861 ttagattcaa acaaactaca tgaattagat gctccagttc ttcaagccga agattttggc
     7921 atatatacgc aacaatatcc atgtgtattt ttctttttag gtgtcggtga tacccctgct
     7981 ttacatgatc cgacgttcga ttttgatatg gatgttttag aaaatggtgt agaatggtac
     8041 aaaacgattc tttttacaga aaatcttctt gtaaaaatcg atagcagttc aaaagaggag
     8101 cgagtaaata atgatgaatt tatccaatcg atttagtcct aggctttcta gaatagaagt
     8161 atcaaaaata cgacaattcg atcagcaaat atcgtcgata ccagatgtga tcaaactcac
     8221 cctgggagaa ccggattttc caactcctga acatgtcaag caagcaggaa tagcagcgat
     8281 tgaagaagac ttttctcact ataccggaat gagaggatta gaggaactgc gggaagcggc
     8341 ttgtatattt cagcaacagc gttatggatt aacttatgat ccgcaaacgg aagttttgac
     8401 gacagttggt gcaacggaag cgattgcctc tgcgttgtta tctgttttgg aagaaggaga
     8461 caaggttctt attccagcgc ctgcttattc aggatatcag ccattagtgg aactagctgg
     8521 tgctgaattg atcccgatag atacttctga cacagggttt gtctgtcagc cggaacaatt
     8581 tgaacgagca tttgaacaat atggatcagc tgtcaaagca gtcattttaa actatcctaa
     8641 caatcctact gggacgacat tgtctgcaaa acaattgaaa gcaatcgctg aggttttaaa
     8701 aaaatatccg gtatttgtta taagtgatga agtatatgct gaattgactt atagtggaac
     8761 gcatatgtcc atagctactt atcttccaga acaaacaatc gtgataagcg ggttatccaa
     8821 gtcacatgcg atgactggtt ggcgagtggg ctttattttt gctcaaaaag cccttattga
     8881 cgaactaatt aaagtccatc aatatctggt gactgccgct acaacgatgt cgcaaaaagc
     8941 tgcggctgaa gcgctaataa atggggtgaa agatagcgag aatatgaaag agcaatatca
     9001 aacacgaaga gattatctgg tccagcaatt ggcgccttta ggatttgaag tgacccagcc
     9061 aaacggtgca ttttacttgt tttgcaaact cccagctgcc attcaaactg actcttggca
     9121 gttttgtgta gaattagcgg aacaggcaaa agttgcgtgt atcccaggaa ttgcatttgg
     9181 cccagaagga gaaggttata tccggatttc ttatgctagc agcatggaaa aattacatga
     9241 ggcctgcaaa cggatcaaag actttttaac agaacagtga ttcatagatt ttatcaaaaa
     9301 aacaatcgtt aattttgcgg tatattctgc aaggagacga ttgtttttta aatttagttc
     9361 aaatgaacgc gtttcttttg attcataact aatgtaatgt taagtttaaa ttgtttagaa
     9421 tgattttaaa agaatattca aggaggtttc atcaatggaa aaaatcaaag ttgtcattgt
     9481 aggtgcatct catggagggc atcaatcgat cttggaacta ttgacacgat acggtgaaaa
     9541 tgtagatatc acgctatttg aagcaggaga ctatatttcc ttcatgtcat gtggtatgga
     9601 gctgtattta gaagacaagg taacagatgt aaatgatgta cgtaatttca gaccagaaaa
     9661 ctttccgcaa tccaatgttc atattttaaa taatcacgaa gtaaaaacga tcgatgccga
     9721 taaaaaaaca gtaacggtta ctagcgcatc agataacgaa acaaaagaat atccttatga
     9781 caaattgatt ttgagttctg gtgtcaaacc aaacaatctg ccagtaccag gagcagatct
     9841 agaaaatgtc tacttgatgc gtggatacaa ttgggcaacc aaaatcaaag aaaaattaaa
     9901 tgatccggct attaaaaaaa tcgctgtcgt aggtgcgggt tacattggga tcgaagcagc
     9961 agaagcaagc cgaaaagctg ggaaagaagt cgtattgctt gatgtgatcg atagaccttt
    10021 aggcacttat ttagatgctg agatgacaga tattttagaa caacacctaa atgaaaacgg
    10081 tattgaagtt ttgacaaatg caaaaatcga agcttttact ggtaacggaa aagttgaggc
    10141 aatcaaaaca agtgaaaaag aaatcccagc agatctagtc attcaagctg ccggtgtgaa
    10201 ggcaaataca gaatggttaa aagggatcgt cgacttagat gaacgtggct ggatccaaac
    10261 aaatgaatac ttgcaaacga atcttccaga tgtgtatgca gtaggagatg ctacacttgc
    10321 ttattctatc ccagcacgca aaaaaatgcc gatcgcttta gcgactgttg cacgtagaga
    10381 agcacgttat gtagtaaaac atctttttga agaagtacca aacaaaccgt tcggcggagt
    10441 cgtaggttcc tctgcattaa gtgtctttga ctatcatttt gcagcaagcg gattgaatag
    10501 ctttacagca gcaaaagcag gagtagatgt acgagtgtct tattatgaag atacgttacg
    10561 accaaaatat gtaccagaag aacaaggaaa tccaaaagtg tccatccaat tattctcgga
    10621 tccaatgacc catcaattgc taggaggagc agtattatct acctatgatg ttacaggtca
    10681 aggaaatgta ttggcactag cgatccaaaa caaactgact ttagaagaac tagcagaagc
    10741 tgacttcttc ttccagccag gctttgaccg acaatggagc atgttgaatc tagccgctca
    10801 acaagcgtta ggagaagaac catttgtaga ataagaaaac gaaatatttg aaaagaggtt
    10861 gtgaaaaagc tgccttgcat caagtaataa gaacagccaa gtaaaaacag cttcttatgt
    10921 ttttaacttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattttcaa
    10981 aaaagggact gtgacacgat ttgtgtaccg acccccaaaa gttagaccaa aaaatctaac
    11041 ttttggaggt cggtattttt tatggttaaa tatacttttg aacttaaatt aaagatcgtt
    11101 catgattatt tggatggcaa aggcgattgg accgtaaaaa tccagatcaa gagattgaag
    11161 cggaaatgct taaaattcgg gagaagcaca aggattatgg ctgtttacgg atgaccaatg
    11221 aactaagaaa ccgcggtttc tctatcaata aaaagaaagt tcagcgcttg atcaagaagc
    11281 taggaataaa agtaacggca tacacgcgta aatcacgtcg ctacaattct tatcgaggaa
    11341 aagttggagc agtagccaaa aatcggatcc atcgtcgttt ctacaccagt atctgccatc
    11401 agaaaatcac gacggataca actgaattta aataccatga agtggatgcc aaaggcatta
    11461 tccgtcaaaa gaaattgtat ctagatcctt ttatggatat gttcaatagt gagattttat
    11521 cctatcgtat ctctgaaaag cccaatgcat tagcggtaat ggaaggtttg gaggaagcta
    11581 tccagaccac taacgattgt ccctatcgtc gtacgtttca ctcagatcaa ggttgggcgt
    11641 atcaaatgaa agcttataga aacaaattga aggagtacaa aatctttcaa agcatgtctc
    11701 ggaaagggaa ctgcttagat aattcactta tggagaattt ttttggctta ttaaaacaag
    11761 agatttttca cgggaaagtt tataactgtt tcgaggaatt aaaatcagcc atcgatagct
    11821 atatctatta ctacaataat gaacggataa aacagaaatt aaactggcaa agtctcgtcc
    11881 aatttcgtaa aacaaccgta acggttgtgt aaaaatatca ttttagagca taaaaaaaag
    11941 gagtggattt ctccactcca aaaaagtcta acttttgggg gtcactacag tgtcacagtc
    12001 ctttttttag taaaaggata catttcttcc ttgtatattc catgcgtgtt cgtgagaaag
    12061 catatataat ttaattaaca gatagttagg gggagaaaaa atgatttttt caattacaat
    12121 gaaactggtg ttcgggttga tcggtttatt attagtggta cgtttgcttg ggaagaaatc
    12181 catgtcagaa atcacaccgt ttgatttagt ctatacgctt gttttaggtg ggatcttaga
    12241 agaatcaacc tatgatgaca atgtccatgt agggcatgtc ctatttgcca ttgcgctttg
    12301 ggcagtaatg atttatggta tcgaacgtat cgtccaaaaa aatgaaaagg tcaatcgttg
    12361 ggtcaaaggc gagccatctg ttctaatcaa agacggcgtc atcaacatga cagagttgac
    12421 aaataaccat atcgagatgg agcagttgcg agcaatctta agacagcaag aatgcttttt
    12481 tcttgaaaat gccaaacatg ttatcctaga gaatgccggt caaatgagtg tattgaaaaa
    12541 atcagatgaa gacaaagctt tatcgatcct gcttgtagat gaagggcaaa ttcagcataa
    12601 agtgcttcag tccaatcaat tgactgaagc atggcttatg gagaacttga agaaagaagg
    12661 ctatgcagat gtaaaacagc tgatttatgt tgaatggtca gaagaaaaag ggctctacat
    12721 actaaataaa gaagacgtca tgcatgaatc ttaccgaata gatggataga tagacagagg
    12781 ttgtgaaatc agcgccttgg cttgttactc aaaactaagc gactttttca caacctctga
    12841 tatctaatat acgttgttcc agaagttgct tcttcgaaaa ataagccaaa attccacaaa
    12901 aatttaagaa gcaattttcg aaaaatcctt tcttattttt tgaagttgat cacttctgtc
    12961 acaacctctc atgtcacaaa ccattttttt gtttccttta actcaatata ccgtacgtct
    13021 tcccacaata tcggctgatg gtcaatcata aagtaatctt cataggcttc accaatgaag
    13081 tgcccttcga cctcatccaa gagtcttcca aattcatctt ttaaattgag ctgaatgatt
    13141 actgctttac gagataaaaa ggcttctgtc agtatactat cgatttcttg caatgtcatt
    13201 tgttgtttag gaagaggctg tttgcgtgct tcttcttcat tttttttgat accgttgact
    13261 agttcttcca tcgcaaaagc agtcccccat tttaatccaa atggccgatc tcgatactcg
    13321 ttatagtcaa tgaatttttt tgctgtccgt ctcatctttt ccaccttcta atccactcat
    13381 accacctgca tgcccgccaa cgagagaact acgtgcgatg gcgcgtcctc cttgcaagag
    13441 actatgcgca tggatgattg attgaaagcc gtattttttt ctaatcgtat cgacaacaaa
    13501 atcaactttc gcttcattta tctgcttttc aggatcttca aaaaggttaa gctgtaaacc
    13561 tgtcgcgtat acgaggtcgg aacagttcac gcctacacgc cgaatatcct ggtagtggta
    13621 atgctggtgg aaaatggtca ataaagcatc tgctagatct ctgctagcat ttgtcgcttc
    13681 gattttcatt tgctggtgaa acccggtctt gccatttgga tcaagatagc ctaaagagta
    13741 tcctacccat aagctgacta cttgtgtttt tgctgcagat cgtctcaagc gagtgccgac
    13801 ttggtcggcc atttctttga taacgatctc gatttcttct cgtcttgtat aatctctatc
    13861 taggatttga ctatttccga tactttttga ttttggtgta tattcttgtc ccaaaaaact
    13921 tcgatcgatg ccccaagcat gggcataaag ctgagtaccg atgaccccta gcttgctttt
    13981 caagtgataa taatccgcgt gagctaaatc ataaacagaa tgtataccca tttgtttcag
    14041 acgttttgct gttcgtttgc cgattcccca aaagtcagtg atatctgaga ttttccataa
    14101 cttttctggt acatcttcat aacgccattc agcaaacatt tgctttccgt cttttgagcc
    14161 attgtctaag gctagtttag caagtagcgg gttatcgcca attcctatag tcgtgtagat
    14221 tccggtctgc tcaaacacat ctttatggat cagtttagcc atgtcgtacg catttgtttt
    14281 accaaagagt gccatgctat ttgttacatc aaggaagctt tcatcgacgg aatagacatg
    14341 atggttcgct tcgtctgcat attttttata gatagaattg atttctctgt tcttttccat
    14401 ataataggcc atccttgggg caacaatata taaatcactt ggatagggga agggaagatc
    14461 tctggcacgg ctgatattgg tgatgccgta agcttttttt gctgcaggac tggaagccaa
    14521 gattaatccg ctaccccgtg tttctgtcga atcggacgga taggacatga ctactaattt
    14581 agttgtaagg ggatcaagat ccttttcaac gcactctacg gaggcgtaaa aagatttgca
    14641 gtcaatacat aaaatatctc ttgaaggttc tttttcataa acaaataccg gattttgatt
    14701 cgcccacata acaatcacct cttcaacaca attagaacac acgttctctt tccgtgtcaa
    14761 taaaaaacag caattttcaa atacgagcaa aaggctcaca tttggaaatt gctgagaggt
    14821 tatttttatc tatttgtttt tagctgagat cgttgatttt tttatcaaac atgaacagct
    14881 tctggatcga tctttgttgt cccatttcca atgtgtgcaa cataaaattc cggagcatag
    14941 ccgacaactt tcatgtattc atcatagaca ttgtttttga aggcaggtat attttcttct
    15001 ttgacaagag caatcgcaca gccaccaaat ccagctcctg tcattcgtgc acctaagaca
    15061 ccttcttgtt tttgagcagc atctactagt gtatctaatt cgatcccagt tacttcataa
    15121 tcatagcgaa gagagtggtg agaatcattt aacagttgac caaatgcttc taagttccca
    15181 gcttctaatt cagcttttgc cttcaaagtc cgctgatttt cagaaacggc atggcgagct
    15241 ctgcgtatca atgtatcatc gccgatttgg tcaatagttg attcaaattc ttcttcatct
    15301 aagtctccta aagaagagat aggcaaagtt ttttgcaaac gtgccaaggc ttcttcgcat
    15361 tcgctacggc gttcattgta ttttgaatct gccaattccc gccgcttatt cgtattcatg
    15421 atgacgattg cataaccatc tagtttcact ggaaccattt cgtatttcag cgtatttgta
    15481 tctaataaga ttgcatgatc tagctcaccg aagccgatgg caaactgatc catgatacct
    15541 gaattgacac cgatgaattc attttctact tttttacctg tttggactag ttccaagcga
    15601 tctgttttta aatcaaacag atcttctaat acaacaccta ctaataattc taatgaagcg
    15661 ctactggaaa gtcctgcacc attagggatt gttccttcca ctaataactc aaacccatga
    15721 tcgattgagt gtccagcttc ttttaattta aggaccattc cttttacgta atttgcccag
    15781 tttgcttttt tatcatattc tagctcatct aaagtaaaac taatgacacc gatatcgtca
    15841 aagtttgtgg aataaactaa tattttatta tcgtctcgag gtgctgctac accttgcgtt
    15901 ccataagtga tggaagcggg gaagacatgg cctccattgt aatctgtgtg ttcaccaatc
    15961 aaattgatgc gtcctggtga gaagtattgt gtagtttttt taggtccgaa tgtttcggca
    16021 aattttgtgt ttaagcttct tttgatttcc atgatgagtt attcctttct tgttttgatt
    16081 ttgaattctg tcactgtttc aaatactgat ccggctttta aatgaatcga accaaatgct
    16141 gagaagcgct ctgcacctgg agctgtttga gtctcaaatg tgatgccacc gtgatgtgcc
    16201 agttttcggt tgcgcatttc tggtgtttct gtgccgaaat ttgcagtgaa aataaccaca
    16261 ctagaagcat ctgtagcaat atctacttgg attcttttat cggggctagt caagcgagcg
    16321 gcttctttac ccaagccggt ttcttttaag aaaaatggat gatcgattcc gtcaacaaga
    16381 tttttttgat ccaaatcact agcaaaaacg gacgctaatt ttttaggtat ttgaaaatcg
    16441 aaagcacttc ctgcagcatt ttcttttacc cctataggaa tactatctgt tcgcaaagga
    16501 gcataagctt cgctattgag ccaaagctca tgttgatcga ttgactgaga agcatctccc
    16561 gtcaagttga agtaaacatg atttgtcgga ttgaataaag tatcttggtc actggtgccg
    16621 cgagaggtca ctcgccagat attatctttt gtcaaagtat agcggatttc cacatccatt
    16681 gtacctggaa aaccatgttc tccgtcggga cttgtagtag taaaaatgac agaagcttca
    16741 ttttcgccat tcaaaatgac ataagaccat ttttttaatt caaatccagg agcaccacca
    16801 tgcagattat gtccagtttt tggatctgtg gctaattggt aagtttttcc attgagagaa
    16861 aagtggccat tttcgattct ccctgcggtg cgaccgatag aagcaccgat atacggatct
    16921 ttttcgatat attcttcagc ggtgtcaaat cccaaaacta attctcgatc atttgatttt
    16981 aaactgacga ttctagcacc taaatcagag atagaaagtg ctagcttgtt tttgttctcc
    17041 aaggttatca agtgataacc ttggccaaaa tctttttctg taatcttcat tttcaagatc
    17101 ctttcttaat gggaaggaac tactgcatcg tgaagttcat cttccattgt atcagctgga
    17161 gccaatttgt tttccaattt ctcaacgatt tgtgcatgtt ttttctctgt caatgtaatc
    17221 ttccaagcaa aaataatagc ggagaaaatc atcaaagcag caggagcata gaatgcataa
    17281 gctttgaaag tcgttacgcc atgagcagtg atatcagcag cagtagcatt accagtcatc
    17341 ccagaagcga tagcgacaaa ccctacgatc ccgttagaca aagcaccagc gattttatct
    17401 agtaaagggc gaattgatag agtaacagct tcgttacgga taccattttt ccattgacca
    17461 tattcaacag agtctgtgat cgtcatcaaa gcggataaga aaatcaattg ttgagggaag
    17521 tagaacaata ctagcccgat gataacgatt ggcaaacttg aacctgcgat cgtaaagaaa
    17581 aagtaagaag aaatcatcat tgcgatccct ccaaggtaaa cgtagcgacg agagatgacg
    17641 cgtgtaagca ttgggaatag tggaacagct actaaaccaa gtaaggcagc aactagacca
    17701 acgatccaaa attcagttgg ttggccaagt acatatttga agaagtagaa taatacggca
    17761 gttgttgcta cattggcaat cgcataagcg atataagcaa gggcaagcca catcaactga
    17821 tcattttgag caactgcttt gaacacatct ttgatcccag ctttttcatt ttgctgacgg
    17881 atcgcagtat cgacttcttt tgtgcccaag gcagtgataa tcgcagtacc gcctgaaata
    17941 acagcagcaa tcacaccgaa ccagaaccag cctgaagcgc cttcaatatg tttatgagta
    18001 acaagatagg taaagaaagt aacgataggg acaacgacta gagtagtacc atttgcacct
    18061 agagaagagc cgaaacgagc aaacgtagca aatttttcac gttcctttgt ttccgtactc
    18121 aaagctggaa tcattgacca aaaagcgata tccttaaatg aataaaaact atccaataag
    18181 atgaaaacga ttgcaaataa tatgataaat aaagtcgtat tatgagtagc taagccaaag
    18241 aaattggtaa agattgctac caagagtacg gaactaacaa cgccgccgat aactaaccat
    18301 ggtttgaatt taccgtattt tgtacgagta ttatcgatga tgccaccaat caaaggatca
    18361 aaaataattt caactaagcg gatacctaca actaagcttg taactgtagc aatcattttt
    18421 gcattcgttg ccttatctgt tcctgcgaac atctggcttg tcacgaatac cataaaataa
    18481 gtgcttaatg taacatagaa tgtgtcgtgt ccaaaagcgc ctaaagcgta ggacacacgt
    18541 tggatcattt tgcttttcat aatctgtatt cctctcctgt tcaatatata atactaagta
    18601 aaccggttac aacgaataac aaagatcttt taatgactta aacttaacaa gaaatcgctt
    18661 cctttttaat atatttctgt gacttttatt taactttttg ttgcattata ctaaaggagt
    18721 aaggagggat agacgtggag caatcgattt ttttaaaaaa ggaacatcaa caagtagaaa
    18781 aagaattgta ttttgatttt tgtggtttct ctaaaacgat gccgtttcat tcttttggtc
    18841 ctgctattcg caatgattac atcctgcatg tcgtgatgga aggcaaagga tcttatcatg
    18901 tgaaaaatca acaattccaa ttgaaaaaag gtgatctgtt cttgattcgt ccaggagact
    18961 cgacgtttta cttatcagat ggagaagatc cgtggatgta ttgctggctt tcatttggtg
    19021 ggagtgctgc agaagaaatc attcggcagt cgcttttcag agaagaccaa tacacgatgg
    19081 tctcttctga catacaaaaa tatatcgaca ttattttaga atgcatgaaa gatacggaag
    19141 aaaccttgga aaatgaatta cagttaacag cattaaccta ccgtttttta tcagtccttt
    19201 taaaagatgg cggaaaggtc agcggtggtg tacaaaaggt tcattcctct ttagctgttg
    19261 aaacagtcaa atacatcgag gcacattatg ctgaaaaatt gtctgttgag gaaatcgccc
    19321 agcatttagc ggtcaatcgg agccatttgt cgcgagtatt caaaaatcat atgggtacca
    19381 gtatcaagga atatttgatc ggggtacgga tcaatcgagg ggcgtttctt ctttctctga
    19441 cggatgaatc cgtggaaagt attgcctatc aagtagggtt caatagtcta gtcgtattta
    19501 gccgaatgtt caaaaagagt accggagaaa cggctacgaa ctatcgtaaa cgtatgaaaa
    19561 acgaaaattt taagaatatt gctttggata ccttgaaaaa acagttggaa aaacaaaccg
    19621 cagtttcttg gacaacctga ttagtctggt taaaatgaca agcatcggca aaagaaactt
    19681 gtggcggtta tgctacactg taaagcgaag actaaaaagg aggaaaaggg atgcgtacac
    19741 ttatcatcat tagccatcca acgatcgaag aatctttaaa tcagcaattt ttcaaagaag
    19801 cttctgctgg tttaccagct acttggcatc atttggaatc aagttatcca gacggaaaaa
    19861 tagatataga gactgaacta cagctcttgg cccagcatga tcggatcatt ttccagtttc
    19921 cgttttactg gtatagttct cctgctcatt taaaaatttg gcaagataat gtcttggaac
    19981 gagcagcaca tgtattaaaa gggaaagaac taggaatcgt attgacaaca ggtgtggcgg
    20041 aaaaagaata ccaagctgga ggaaaagaag aatatacgat ttccgaattt ttgaggccgt
    20101 atcagcgaat cgcaaataaa ttccatatga cctatcttcc gccattcgtt ttagcacaat
    20161 ttatgtattt atcccaggaa aaacgttggg agaagctaat tgcttatcag cagtatctct
    20221 cattggaagg caaaccatcg ctgactcagc ggattgactg gtttattcaa agagtacagg
    20281 aaaatcagaa aatgcaggaa gaagaatctg aaaaacaagc atatatcgta gaagcactga
    20341 ctgaggcaaa agagcagatc gaagacttga cctttacact gcaagaaatg aaaggaactt
    20401 cattatgaca aatgattggc tgatccaaca attagatgaa ttaagcgaaa aatcagaata
    20461 tcaagaccgt gcgctcttat atgaaataaa gaaactgatg gctgagatag acgaacgaac
    20521 aatgcagctg caaggagaaa tcgacggaca atcttggaat catctcaact ggtgaaattt
    20581 tcttataaat aacaaagata aaaatgtgca cagcgacaat tatttgctac aataagaata
    20641 tgaagcgttt gataacgcta ttatacataa aaattggtgg tgaaaaaaat gtatgatgaa
    20701 aatgagtggg gagaaaatgt tcggatgacc cgaagaagac agcaaaaaca ggcagctcgt
    20761 aagcggatga tcatgattgg ggcagcagtt gccgtgtttt tgatcatagc aggaggagtt
    20821 gcgggtctat attttcatgg aaaaaattcg tcagaagcga aaagtacgac cataacggaa
    20881 acaacgacaa agaaaacaga gacgaaacaa acaagtgagt catcaactaa aacaaaagag
    20941 acacaaactt ctgaagaaaa aacagcaaat ggtttgccag agcaacttca agcaatcgta
    21001 aatgattatt cgaaaaagat cgaagaaaag acgcctgcat tgattgaaga ataccagaca
    21061 gaaattcagg gaaatcaaga gggaatagct ggtttatcag cagttgctaa ccaaaaagca
    21121 cgtgagcttc aagcaatctc cgacgaaggg atcagaaaat tacgtgcagc gtatcaggca
    21181 gcagaaaata aagacggtgt agacttagat actttgatca atcaattatc ggctaattat
    21241 acgaatcatg tcgctaagat ttccgatatt tatttgcaga ctagcgcctc tcttcaagca
    21301 gagtcaacaa gcacacaaga cacaacatct tctgattctg agacagcaga gtcggtagaa
    21361 gcttctgaaa tgcaagctcg aacaacacaa gacacaacag attcctctga aactaatacc
    21421 aacagcgcga cttcacaggc tgcgacaacc gtagttttgg aaggagaagg tccaaatcaa
    21481 atagccgaac gaactggcgt accagtagaa acgatcttgt cgctgaatgg gatgagcatg
    21541 gagaattatt tcctgactcc cggtgagacg ctaaaattga attgataaaa gcaataaaaa
    21601 agaacgaagc ttccactaag gtgaagcttc gttctttttt tattttccgg ttcttttttc
    21661 gcgtacaaag taatggatcg tcatgtaaag caagaaaacg agtccaataa tcaatgagat
    21721 tctcgtttca ggattgataa acatgaaaat caatacgata gctaaaaaag caagtgtcac
    21781 ataattcgta taaggagata gtggcatttt aaaaggatga tcatgcattt tatctgcgtg
    21841 cttctttttg aagcgaatct cgctgatcaa aatcactacc caaggaacca taccaggcaa
    21901 aatacttgag ctgtatacga aaacgaaaat attttctgac tggttgatga acattggtag
    21961 aagataattt aagatcagac caattaagat tccacctgag attgctacga caggataaaa
    22021 aggcgcaccg tgacgagata gtttcagaaa ttttttagac atctttccat ttatggcaag
    22081 tgtatagacc atacgacttg cgctgaaaat ccctgaattg caacctgata atgccgctgt
    22141 aatcacgaca aaattaatca gtcctgcagc aaatgtaatg ccgactttag aaaaagtttg
    22201 tacaaaaggt gaaccaatct cactcaattg attccacgga tagatactca caatgataaa
    22261 aatagcacca atataaaaaa tcaagattcg accgatagtg gactggacag cttcaatgat
    22321 cgtcttttta ggattctctg cttcaccagc agtcattccg atcagttcga ttccttgata
    22381 ggaagcaaca acgatcgata atgcaaagaa aaatcctttg acgcctccag tgaagaatcc
    22441 accatttttc caaagatttg aaatccctac tgcctgacca tgattaccaa atccaaataa
    22501 gattacccca agacctgcaa tgatcatcaa aatgatcgtt atgactttga tgatcgaaaa
    22561 ccaaaattct aattcgccaa acgccttgac agaaataaga tttgccagca taagaaacaa
    22621 gacgacaatg attccgggaa taattgccgg caaatcaggc caccaataat tcatataaga
    22681 tcctacagca atcacttcac ttattcctac gacaatatat tgaaaaatat tactccaagc
    22741 ggtcaagtag ccagccacag gatggatgta ctcgctcgcg taatttgcaa aagaaccagt
    22801 cgaaggatca acgtaaagca tttcgcctaa agcacgcata atcaaataca aaaagaatcc
    22861 tgcaatcgca taggctccca tcactgaggg ccctgtccaa cgtatggttg aggaggcacc
    22921 cataaacaga ccgacaccga tcgttccacc tagtgcgatc atttggatat gacgggcact
    22981 aagacttcga gttaactttt cttcttccat acaaaaactc ctttcgttgt tgagtcaaca
    23041 agaaaaaata atcaaacgtt tcaacacatg tttttcatta ttaggttagc cttattgaac
    23101 tcttgatgtg attctacaga aaattttcat aaaatgcgag gaaaattcga aaaaaacttt
    23161 ttcatcattc tacttgaaaa tgctttccat tttagttaaa ctatatttaa ataaaaagtt
    23221 aggtaaacct aaaaaggagt gtaagatttg gaacgtacgg ccattgtaat tcaagacctt
    23281 tcagccgctt atcaagggaa aactgttttg aaaactatca acttgacgat aaatccacaa
    23341 aaaattaccg gtattatcgg accaaacggt gcaggaaaat caacatttat gaaatctttg
    23401 ctagagttga taccagctac tactggtaaa gtcacctttt ctggacagtc ggtaaactca
    23461 gtccgtaaaa aaatagccta cgtggaacag cgaagtgaat tggatctttc ctttccaatt
    23521 gatgtattag gcgttgtact tttaggaaca tatccatctt tacgaattgg acaaagacct
    23581 gggaaaaaag aaaaagaacg tgcaagacaa gctttgaaaa aagtagggat ggaagaatat
    23641 gccaaaagac agatcagcga actatcgggt ggacagcttc agagagtttt tattgcaaga
    23701 gctctagccc aaggagcaga atggatcttt ttagatgaac cattcgtagg gattgatgcg
    23761 ttaagtgaac gaaagatctt tgacatcttg caggaattga agaattcagg aaaaacgatt
    23821 ttgatcgtcc atcatgatct tcataaagta gaagaatatt tcgatgaggt tattctttta
    23881 aataaacagc tgatcgcttc cggtccagta caagaagtct ttacatcgaa gaaccttcaa
    23941 ttggcctatg gtgagatcat cagacattta gtaaaaggag gagagaaaaa atgatccaat
    24001 cctttattga tggattaatg aattatcaat tcctgcaata cgctttggtc acttccatgc
    24061 ttgtaggact tgcttctgga gtcatcggtt cttttattat tttacgaggg atgtctttga
    24121 tgggagacgc tatctctcac gcagttcttc caggtgtagc catttcttat atgtttggtt
    24181 ttagttacat ctttggagcc actgcttttg ggatgctaac agcagcagtg attggtttcg
    24241 tgactcagcg gagccgattg aaaaatgata cagcgattgg aattgtcttt agctcctttt
    24301 ttgcattagg gattatcttg atttcatatg cccagagtgc gacagatttg tatcatattt
    24361 tatttgggaa tgtcttagct gttagggaaa gcgatttgtt gctgacagca ctcgttagtg
    24421 gaatcgtctt gatatttgtc tttttctttt ataaagaatt gaagatcaca tcattcgatc
    24481 cgacaatggc aaaagcttat gggctgaaca catcgctgat ccattatctt ttgatgttct
    24541 ttttgacatt agtggctgta gtcagtttac agacagtagg aacaatcttg gtgattgcca
    24601 tgttgatcac accagccgcc acggcttact tgctaacgaa ccatttactg aaaatgatca
    24661 ttacagctgc aggaatcggt atgctaagtg cagttgtcgg tgtgtttttc agttatagtt
    24721 acaattggcc atcaggagct acgatcgtgt tagcatgtac cgcatttttt atccttgctt
    24781 ttttattttc tccaacaaaa ggaattttat ttcagaaagg aagcagatag atgaaaacaa
    24841 aaaaatcact ctttttaatc ttggcagtta gtttcttggt attagccggt tgcggaaagc
    24901 aggcttctga ccaagcggat gaaggaagta aagaaaagtt gtcagtcgtg gctaccaatt
    24961 cgatcttggc ggacatggca aaagaggtcg gaacagatca aatagatatc cacagtatcg
    25021 taccagtcgg aacagatccg catgaatatg aagtactacc agaagacatc aaaaaggcaa
    25081 gcgatgcaga tgttatttta tacaacggtt tgaatcttga aacaggtaac agctggttcg
    25141 ataacttgat ggaaacggct aaaaaagaag aaggaaaaga ttattttgca gttagcaaaa
    25201 atgtagaacc tctttattta actagcggtg aagaacatac aaaagcagat ccccatgctt
    25261 ggctagacct atctaacgga ataaaatatg tggaggaaat cgcacgtata ttctctgaaa
    25321 aagatgcaga aaatgcgaca ctctataaaa aaaatgcaga agcatatgtg gaaaaactaa
    25381 aagaattaga tacacaagcg aaggaaagtt ttgcttctat cgaagagaac aaaaaattat
    25441 tagtaacaag tgaaggggca ttcaagtatt tttcacgagc atatgatttg ccagcagctt
    25501 atatatggga gatcaataca gaaagtcaag gtacgcctga tcaaatgaaa gcgatcattg
    25561 atcagataag agcaaaagaa gtaccggttt tattcgtgga aaccagtgta gactcaagaa
    25621 gcatggaacg ggtagcaaaa gaaacgggtt tgaaaatcta tgataaactg ttcactgatt
    25681 ccatagcaaa agaaggagaa caaggagatt cttactatca gatgatgaaa tggaatattg
    25741 aaacgatcca tgaaggtttg tcacaaacaa aagaaagctg aaaagtgcaa ggtctagctt
    25801 gaggagcaga ccttgcattt tttataagag atatgaaaaa aaattatgct gtttttcgtc
    25861 gtacagatac cttttttgaa gaaattccgt tatagttaga gtaaggaagg aaggtgtggc
    25921 tatgaatttt acacgtaaag ataaatatac gattaaagaa gaagtctttc gattgatcag
    25981 ccagcaggaa aataccgaca aggatcaaga ccttcaattt cttgaagcag aattagctaa
    26041 attattcata gcaaatcaaa atgagacaac agaaccagat gaagagctgt acataggcaa
    26101 atggttgttt gatgaatttc agcctttaga tgaaaatacc ggtatcggtt attttgtgtt
    26161 cctttctcca gtcggcttca cacctggcgt attagttaaa caaaacgacg aaataaccgt
    26221 caaagcatgg cctgcattat tttcagtcat caaagcagaa ggagtaatca aaaagatcca
    26281 gtcggcctat tataaaagtc aatttattgc caacagcaaa ttaatgaata tgttttataa
    26341 tcgtctagta gaaattgcta ggcctgggaa aatcccgcaa acattcacgt acacacagca
    26401 aaaaaatctc tatgaagaag tattgtattc ccgcgatctg tctgagtttt cgccatggga
    26461 agaggtaagg ctccatgaac tgttaagtac aaaaccagtt tcattgaatt tttttaatgc
    26521 attagataac aaaagaaaat taatttcaac aaattattgg gaagtatttg ctgcaagaaa
    26581 aaaatggatg gaagaaaaga agattgccga tcagcagatc cagtttgtcc agctgaaaaa
    26641 agaaaaacta tattatgggt tatacagccg ttaacagcaa gcgaggaaga ccatgacatt
    26701 aaaagaagaa atcatacaag aaagcaaacg attaggaatt gacaaaatcg ggttcaccac
    26761 ggcggaacct tttgattcat taaaagaatc gttggaagaa caaaaagcag ctggccatac
    26821 ttctggcttt gaacatccaa atatcgatga gcgtctttat ccagaaatga catttgatca
    26881 gccgaaatcg atcatttcca ttgcacttgc gtatcctact aggatccatg aagaagtgcc
    26941 tagggatgaa aaacgcgggc agtttgctcg tgcttcttgg gggatcgatt accatgatat
    27001 actcagagac cgtttgaacc gattgatcga attcatccaa acaagagcag aaaaatggca
    27061 aaaagaagag gaatggcgtc tagctccgca agtagatact ggagaactga tcgatgtcgc
    27121 tgtagcacag cgagctggtt taggatttat tggacgaaac ggattgttga tcactgaaga
    27181 gttcggttca tttgtctatc ttggtgagat cgttactaat attgtatttg agcctgataa
    27241 accaggagaa tttggttgcg gtgactgtat gcgctgcgtg actgcttgtc cgacaaaagc
    27301 attgcttggt gacggaagaa tgaatgccca gcgatgtttg tcttatcaaa ctcagacaaa
    27361 agggatgatg ccggaagaat atcgtaaaaa aatgcgaaat gtcatttatg gatgtgatat
    27421 ctgccagtta gtctgccctt acaatcaagg aaaagatttt cattttcatg aagagatgga
    27481 accaaaagta gacgaaatat atccaaaatt aaagccaatg ctttcgatgt ctaataaaga
    27541 cttcaaacag caatttgggc atttagctgg ttcatggcgt gggaagaaac ctttgcagag
    27601 aaacgcattg attgctttag ctaatctagg tgatcgaacg gcattgcctg agatttcaga
    27661 atgtttaaca gatgtccgcc ctgtcatcag aggaacagca gcttgggcta ttggacgtct
    27721 aggaacaaaa gaaccggaaa aatggcttga tgtattagat gaaatattag ctaaagaaac
    27781 agaagaagtc gttataaacg aattggaaca tgcccagaaa ttattgcgga gaaagtt
//
LOCUS       DAZUMM010000029        27825 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000029 DAZUMM010000000
VERSION     DAZUMM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.029"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..628)
                     /locus_tag="ACG8E6_001230"
                     /pseudo
     CDS             complement(1..628)
                     /locus_tag="ACG8E6_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            707..883
                     /locus_tag="ACG8E6_001231"
                     /pseudo
     CDS             707..883
                     /locus_tag="ACG8E6_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1059..1628)
                     /locus_tag="ACG8E6_001232"
     CDS             complement(1059..1628)
                     /locus_tag="ACG8E6_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5061660.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1702..2991)
                     /locus_tag="ACG8E6_001233"
     CDS             complement(1702..2991)
                     /locus_tag="ACG8E6_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061661.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            3318..4193
                     /locus_tag="ACG8E6_001234"
     CDS             3318..4193
                     /locus_tag="ACG8E6_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5061662.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4207..4530
                     /locus_tag="ACG8E6_001235"
     CDS             4207..4530
                     /locus_tag="ACG8E6_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061663.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4527..5279
                     /locus_tag="ACG8E6_001236"
     CDS             4527..5279
                     /locus_tag="ACG8E6_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFS5061664.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5269..7440
                     /locus_tag="ACG8E6_001237"
                     /pseudo
     CDS             5269..7440
                     /locus_tag="ACG8E6_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7552..9723)
                     /gene="copB"
                     /locus_tag="ACG8E6_001238"
     CDS             complement(7552..9723)
                     /gene="copB"
                     /locus_tag="ACG8E6_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFS5061665.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9743..11929)
                     /locus_tag="ACG8E6_001239"
     CDS             complement(9743..11929)
                     /locus_tag="ACG8E6_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5061666.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11929..12138)
                     /gene="copZ"
                     /locus_tag="ACG8E6_001240"
     CDS             complement(11929..12138)
                     /gene="copZ"
                     /locus_tag="ACG8E6_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFS5061667.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12151..12591)
                     /locus_tag="ACG8E6_001241"
     CDS             complement(12151..12591)
                     /locus_tag="ACG8E6_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFS5061668.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12665..13204)
                     /locus_tag="ACG8E6_001242"
     CDS             complement(12665..13204)
                     /locus_tag="ACG8E6_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HFS5061669.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(13819..15285)
                     /locus_tag="ACG8E6_001243"
     CDS             complement(13819..15285)
                     /locus_tag="ACG8E6_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5061670.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(15596..17677)
                     /locus_tag="ACG8E6_001244"
     CDS             complement(15596..17677)
                     /locus_tag="ACG8E6_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5061671.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(17819..17941)
                     /locus_tag="ACG8E6_001245"
     CDS             complement(17819..17941)
                     /locus_tag="ACG8E6_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061672.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEKKKKE"
     gene            complement(18201..18560)
                     /locus_tag="ACG8E6_001246"
     CDS             complement(18201..18560)
                     /locus_tag="ACG8E6_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFS5061673.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(18590..18931)
                     /locus_tag="ACG8E6_001247"
     CDS             complement(18590..18931)
                     /locus_tag="ACG8E6_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061674.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(18928..19602)
                     /locus_tag="ACG8E6_001248"
     CDS             complement(18928..19602)
                     /locus_tag="ACG8E6_001248"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS5061675.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(20655..21953)
                     /gene="asnS"
                     /locus_tag="ACG8E6_001249"
     CDS             complement(20655..21953)
                     /gene="asnS"
                     /locus_tag="ACG8E6_001249"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFS5061676.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(21993..23183)
                     /locus_tag="ACG8E6_001250"
     CDS             complement(21993..23183)
                     /locus_tag="ACG8E6_001250"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS5061677.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(23204..23731)
                     /locus_tag="ACG8E6_001251"
     CDS             complement(23204..23731)
                     /locus_tag="ACG8E6_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFS5061678.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(23778..26567)
                     /locus_tag="ACG8E6_001252"
     CDS             complement(23778..26567)
                     /locus_tag="ACG8E6_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFS5061679.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(26714..26914)
                     /locus_tag="ACG8E6_001253"
     CDS             complement(26714..26914)
                     /locus_tag="ACG8E6_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5061680.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(27366..27686)
                     /locus_tag="ACG8E6_001254"
     CDS             complement(27366..27686)
                     /locus_tag="ACG8E6_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061681.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
BASE COUNT         7822 a         5903 c         4886 g         9214 t
ORIGIN      
        1 ctggatcgtt tgtcgcacgc atcatgacgt atactttctt attagggaaa tcacgcatat
       61 tgaaagatac atgatagcca acatttccta aagtattata agcttgattt acgtctggtc
      121 tataaatccc atcagctctt actctagcta gttcttttcc agtattgtag tccataatga
      181 aaatatactc gtatttataa ttagcaatgt gccatccagc tacatgcaag tttgcgtttt
      241 cgatttctcc gaactgatca atgtgggcgt aatttgttcc atctgtcagc gtagaatttg
      301 cagcacctgc tcgagttgga tcaattactg gtttatcctc cgaagtagtt ggattatcat
      361 ccgtaaaacc atgagccaaa tcataggcta gtttttcttt acttacaccc atttcagaaa
      421 gataaccgta aggatctgta tgatcgcccc aaatattttg tgttacccat aaatgcgatt
      481 tgattcctgg ttggttataa ggagtgtcta atgttaatgg aataccatat ttcattgctg
      541 aatctcttgc caattcaacg tatactttat agttcttttc aaaagtttct ttatcatgtg
      601 tgtgttgtaa ctcgatttgc acaggacttt tggctcacga ggttgtccta ctctacatct
      661 tgctgataga tatgctgaaa ggaaaacacc aacattatgt tttgcttttt cagctagaac
      721 gagacaagca ggaatacctt tagaagatat caaagatttt ttaggtcata aagatgtttc
      781 cactacacaa gtctatgccc atatttctcc cgaagtgaaa aaaagatcaa tgaatcaact
      841 agaaaactat atagaagagc aaataaaaaa gcactcaaat tgagtgcttt tttaagctca
      901 ctatcactaa aactatcact aattttattt ataagcgaac atgtttctat cactgatttt
      961 ttaaatctag cgaaaaaccc ttatcttaaa ggcgttcgtt caattctttg gcaaggtctt
     1021 caaagcctgg cttgcctaga agtgcgaaca tggttttctt atagctcatg tagaaaaatt
     1081 ttcttgatga tgattttatc catcaattga gattgactgt tttgctctcc tatgatttca
     1141 gctatgattg tccgattttg cggttttctt cttaactggt agttgatttt cccagtttcc
     1201 atcaactcgc taagcatatc cacgactccg ccaaatccgg tctttttcgt aataaaacct
     1261 agtgctacca ttcgttcatc tagatttttt gctgtaatcg tcacaccagt aatgagcgag
     1321 tgctgtaatg tttgggccag ctcttccatc ccttcagcat tggatacgac ggaagctgtt
     1381 ttgttaatag gttcttcgtt tatttctttt ggttctttcc tctccacttt cactgtttga
     1441 cgaaggacat ctagttcttg caagagcgtt ttgtattgtt cttgggtcag catcacgcca
     1501 gctacttttt cacgattaaa tatatatacg ccagttcccg cgtctcgcgc ttgagcaaat
     1561 acatccatcg gcgatcgttt cacttctgtg atcgaagatg tgggaacttc taatttcttt
     1621 aattccaatg gatttcctcc tttactaatc gctttttatt atagcacaga ctgacttgtt
     1681 ttgatctttt ttccaaaggt tttatcggtt tttgcctaac cagtaattga tatcggtaag
     1741 aagtctttcg ataccaaatg aaggatggta cagatacgtt ttttgatctt cgttatggtc
     1801 tagtggaaaa aaatgatcgg tgatcactag atctgcatac cgaggcggct tatttcgagt
     1861 acatacatgt atagagatcc gttttcgatg tcttagcgtt ttgttcagta ccccagcaag
     1921 tcttcttgct tcttggtgtt catagcgaaa aaaaaagacg attttcactt caggagggtg
     1981 gacatctaaa taccctcgtt cctggagtgt tttcagtaaa agcatccccc actctactgc
     2041 ggggtgtgca agtgcttgtt ggatcgctag ccgaaattga tatagtttat ctttggaatt
     2101 gggaaatttt tctaatgttt cctgataata acgaacttct tctatcagcc ggtcatggat
     2161 accaattacg taacgaagcc attcttggta aagtactttt atcagctttg atttgctttc
     2221 gctctcaaat tcgctattca gccaaaacag cgaatcaagc ttttcagtca caatcgtcaa
     2281 tttccgatag tcttgtgtct cttctatccg tttccaccgc ttttcagtaa tctggttaca
     2341 gctcaagcgc aaaattgcta gataattttt ttcttttatt ggcaaaatct gctcctctct
     2401 aaatagcaat tcaggaaaca attcaaagaa tagtgttcga ctgtctccta aaatctcgat
     2461 ttctgccgga tcctttcttt gcttcaataa ttggatgaac atctcagaaa aggactgctc
     2521 tccatcatct gcaaaaaaat gcggaaaatc tttcaaaaag taaaacagca gatatcttac
     2581 ttttctttct gaaccaaaga tctgtgcttt tttataagaa aaagaaaggt ggtactcgcc
     2641 aaaccacttt ctcaatcgct cttgcgtaga acgatcgtac ttctgctcgt aaggttcatt
     2701 taatagcaga tttttcagta aacaaaacgg gggtgattgg atcagaaaaa caaacaagag
     2761 ataaggttcg ctatatcctt gatcataaac taactgaacg gttttcttat gaagaatcag
     2821 ctgaatggga cagtcgcttt ccgccaaacg tttcagatgc cgatccactg ttttaacatc
     2881 gattttcagt tcttcagcta aatcagcttt tcgccagatc aaatctttca ttagattgat
     2941 tagtgaaaat aaattctgtg cattttttga taatagtttt tgccatctca tcgatcattt
     3001 ctcacctgat ttctaaacat attgtttgat caaaatgagt tcactcctaa aaagataaaa
     3061 aactcccttt ctatttattt aaatacgcaa atcttcctca ttatttttta ttttactcaa
     3121 tttaatcgta ttctcatttc aaaacgaatg tactttaaaa aaacataata agatcacttt
     3181 gcttgtagga tgtattccct tccattacaa tgaaataaat agcgatttta tgagaaggtg
     3241 atgtgaaaat ggaagaaaaa atagaagaat ccaaacaaaa aatcgatcag ttgatataag
     3301 aattagaaaa agcggtcatc ggcaaacgtt caactatcca attgatgatc gtggccctat
     3361 tagcaggtgg tcatgtctta ttcgaagata ttcccggtgt aggtaaaacg ttactggtca
     3421 aagcactagc aaaagctgta cagggaacct tcagcagagt ccaatgtact cctgatctgc
     3481 tgcctagtga tattcttggt ttttccgtat acaattcaca atcaaaagag tttgaatttc
     3541 gtccgggacc gatctttacc acgatcttgt tggcagatga gatcaatcgt acgactcctc
     3601 gtactcagtc agcactttta gaagcaatgg ccgagaatca tgcaacgatc gacaacaata
     3661 cataccctct agacaatcat ttttttgtct tagctacgca aaatccaatc gagtacgaag
     3721 gaacatatcc tttgccggaa gcacagctcg atcgtttttt attccgacta caaatcggtt
     3781 atccttcttt tgatgacgaa cttttgctac ttttggataa attcgaaaaa gtgttggata
     3841 acttaaatga agtattgaac agttatgaaa tagaagaaat aaaaacaaat gtggcgaatg
     3901 tctttgtcag tcccgaagtg gcatcttatg ccttgcagtt agtgtcagca acaagaagtc
     3961 atgctgccat ccagcttggc atcagtccac gaggaagcct ttcctttatt caggcagcaa
     4021 aagcttatgc tttaatccat gggagaaatt acgtcacgcc caaagatttg caggatttag
     4081 ttccttacgt gttttcccat cgcctgattt tttacgatcg ttcattgaag gaagacgaaa
     4141 agaaacaaat cattcatact cttgttgcac aaattcctat tcctgtgagg tagattgagg
     4201 tagacaatgg aaaaaatcaa agattatttc attaattttg tgttatttct ggtatatttc
     4261 atcattgtgc tttttgctct tgtattcaac aattctttag gctggttctt gttggccttt
     4321 tttactcttt atgtatcatg gagcttgatt tcattaagca gctttctaaa gaaactttcg
     4381 gttacttgta cattgcagcc actcgtttat cgcaaaaaaa gtatgcaggt tattttttcc
     4441 atagcaaaaa agagaacttt ttctttacct ctccctcgtt tacaaatcgg cttcccagca
     4501 atctttgcag aaaaaaacaa gagattttga ttcttacaaa taaaccgcaa gaagtccaat
     4561 tgctttggca gccaaaagaa agaggatgcc atgaaacatt aactgtcatt tttacagcct
     4621 atgatagtct aggacttttc agaaaaagtg caaaaagaga gatctctttt cctgtgactg
     4681 ttctgcctgc tttttgtaag attcaagcag aaaaattaca gagagttctg gaaaaaaaac
     4741 agcagctgaa cgcgtaccaa agaggtcttg attttcggga acatcgcgct tatcgtccag
     4801 gtgattcttt caaccgaatc gattggaaat tgtctgctca tactcaagag tggttatacc
     4861 gtgaatacga ttatcaagaa gaagaatgtt ctcctctttt ttgcttttgg ggttcgccca
     4921 gtccaaaatt tgaacctctg ctagacttgt atttttcttt atggcatttg agaaaagaaa
     4981 agcagccgga acttctgata ttaggcaacc gcgcctttca tggcaaagac cccggtcata
     5041 cctattttgc ttctttggaa gctggcgatg agaaagcttt tttcgcacac ttgaaaaaat
     5101 atgcaaaaaa acaaatcgtc ctctttgttc ctgagaattc tcctgaagtc tcagaagcta
     5161 ttgaaacatt gtcaaaagat cgaacggctt atcttgttta tttcgtagaa aaacaactca
     5221 tggtccaaga aaaagacaag atatgttcat tgccaggagg tgaatggatt gacgattaaa
     5281 cgggaaaaat ggctgcgtgg cgtttactcc tttgtgatac taagtgtagg tgctccgata
     5341 tttgtagtag ctaatcaatt gcaaaacagc taccttctta tcctaactat tggtgtccta
     5401 tgccttattg tctgctgtat tcctaaaaaa tgggtacgtt tgctgctttc tttgccagta
     5461 atgataggtt gtttgttttt atattttcct tcgaagacat tttccatatt atggatctta
     5521 cagttttttt cgtcagttac agaggaattc ttccatctat cccaaggtaa actttattat
     5581 ttgccagaaa agacagcttt ttttctcata atgttgctga tctatttatt tattacattg
     5641 accgtggatt acgactattg gtacgcaccc tttctttctt tgatggctta cttcatgatg
     5701 cttaccgttt ttcggaaaaa agatttactg tacgaaatga ttgcggtagt aactgttttg
     5761 ttcgtgtatc tggcagcaag gcaatttttc tccatccggt tgttgtcggg aaacactcga
     5821 ttccagtcat tgactacagt tctgcttatt tgtttcttaa cttttagcgc tatcttgcct
     5881 ttatatcttc cgaaagtaca ccaatctctc gaggaaacga gtgaaccgat ccgagaatat
     5941 cttaatgatg aagggcttta tgacacactc catgagtatc aattaaccgg aagtgcaaga
     6001 acaggcttca gtgagaatga tgaacagctt ggtggacctt tatatgataa tggcgaagtt
     6061 gtttttacag cgaaccaaaa aggaaatggc tattggaaga tcgaaaataa aaatcattac
     6121 accggttctg gatggataga agatcaggac gcacccatcc aaccaatcca agaattccct
     6181 gttgacatca cgagctacca aaccgttata gaagacaaag aaaacattac attgacccta
     6241 aaaaaacaag ctttcttacc ttatccttat ggaaatacta ccttgactga tttttcatta
     6301 gctagtggac aaacgcttta ctatttgcct gaaagccagc gattcattcc tgtcccgaat
     6361 gaatcgacac gttctgcaac tttacaaaca gaaaggttag tttttacgag agaagaatta
     6421 aaaagcagtc agttaaaagc tgatgattta gaaaatcagc agacaaatct tcaattgcca
     6481 aaaagtttgc ctaaacgtat tgaggacttg gcggtcgaat tgaccaaaaa tgaagcaact
     6541 ttaattcgta aagtcgaagc tgtctaacag tatttaaaaa caagcggaaa tttccgctat
     6601 tccaaaacgg ataccgggca tactccgaaa gaacacgatt atgtggatta tttcctcttt
     6661 gattcaaaag tcggctattg tgacaacttt tcaacttcta tggtcgtcct tcttcgaaca
     6721 cttggtatcc cagctcgctg gacaaaaggt tttacaaatg ggctcagaac aggcacagat
     6781 aatcaaggga atgaggaata cagcattctc aatagtcatg ctcattcttg gccagaggta
     6841 tactttcctg gatttggctg gcttccattt gaaccaaccc cttcatttgc caatccaatg
     6901 ggttcacaac cagttgctga aacaaccgaa accgtccagc ctgctatcga acaaccaaat
     6961 gaaagcgaaa atatggcaga aactatttcc agtgagagat ctgaggtcat tccatcagaa
     7021 gaaacaacac tcacaccagc ttataaaatc gtcttattca gccttggagt gtgtttatcc
     7081 gtcttgttgt tctttctatt gttattcaga aaatctctgc gctggacaag ttatcttatc
     7141 tatcttcatt tttttgcttc tactaatacg attgcatatc aaaaacttct taaccctttg
     7201 gaaacattgg taccgcgcaa acccgcagaa tctttgcagg aatatagcct gcgaatagaa
     7261 caagaattca agctagatct gcgtttcatt cgattaacta ctcaatatga agcagaaata
     7321 tatggacgac aagcagatcc ggtaaaattc gataaaaaaa cctatttaga aataacgcgt
     7381 ataataagaa aaacggtata caaaaaaatt ctgacaaaag ggagccgctt caaaaaataa
     7441 gataaaattt ctggagaatc tacttctgga atgccgagaa ccagctgatt gagtatcgat
     7501 tactcgaaaa aaaggttgtg acacgttttt tgtcacaacc ttttttcatt attatttgat
     7561 tttcaatgtc aaagcattta gagctacaac taccgtactt aacgacatca agacagcacc
     7621 aactgctgga cttaatatga taccaattgg cgcaagaatt ccggctgcta gcggaattgc
     7681 aagaatattg taacctgccc cccaccagag gttctggatc attttctttc gcgtttgctt
     7741 ggctagatca agaaaatgca aaatatcttt tggatcgctg tttgtcaata cgacatccgc
     7801 agaatcgatt gcgatatctg tccctgcacc gattgccatc ccgattgaag cacgggctaa
     7861 acttggcgca tcattgatcc catcacccac catgattact tttttccctt ggtcaaggta
     7921 tttctggaca acagcttctt tatcatctgg taataaaccg ccataatatt cttcgatccc
     7981 taaataatct gctactgctt gggcagcgtt tttattgtca ccagtaagca tgacaggagt
     8041 gatttttctt tctttcaatg ttcggataaa ttcttttgct tctgttttta ctacgtcacc
     8101 taaagcaacg attgcagcta gatgattttc taaaatcagg aagctgatgg tatttccttg
     8161 ttcttggtaa ggtttcaata atgcttgttc tacgtttaat ccgagacgct caacttcttt
     8221 ttcattaact attttcactt gttgtttttt gactgttgcc tctagcccga ctcctgaaag
     8281 attttttaga ttttcagctt gataaggctt gatttcatgt tctttcaaat aattcataat
     8341 gccaacagcc aatggatgat tggcattcgc ttccaaggct cctagatatt gaaggatttc
     8401 atttttattg aacgcatcat caagtacttc gacacccgtt acagtaaatt ttccttctgt
     8461 tagtgttcct gttttgtcca gcatgatcac atctagatca ttggcttgct ccagtgcatt
     8521 gcggtttttc aaaagtaatc cgtttttagc tgctattgat gtggagcgag caacaactaa
     8581 tgggatagct aaacctagag catgaggaca ggcgatgata aagactgtta ccatacgttc
     8641 taatgcatta ggaagatctg tcaagaacaa ccaggcgatg aatgccagga tacccacaat
     8701 taatgcgaca taaaacagcc attttgccac tttgtcagat aatgactcta gcttggattt
     8761 ttctccttgt gcttttcgca ccatttccat aaccttcgct agataaccat tttcaccagt
     8821 tccagttact tcgatttcga tcgttccatc cccattgata gaaccgccaa tcactttgtc
     8881 attggcttgt tttttgattc ctcttgattc gccagtcaca gctgattcgt caacaattgt
     8941 ttcacctttc aagattacac catctgttgg cattttatca ccagaccgga cgatcaaacg
     9001 atcaccttcg tttacttctt tcaatgaaac tgtctcttct tttccatctt tcgtcaagcg
     9061 tttcactgat tctggcagta attctgctag tttttgtaaa gcattgctgg cattcgacac
     9121 agcattcatt tcaatccagt gacctagtag cataataacg atcaaagtag ccaattccca
     9181 gaagaaatcc atgacgtgtg tatgtggatt gagcagattt gcgataaacg aatagacact
     9241 ataaatatat gcaactgtaa tccccatcgc gatcagtgtc atcatagcag gacttttttg
     9301 tttcaattcc atcttagctc cacttaaaaa tggctgaccg ccatagataa atagtatagt
     9361 tgctaagatc aatacgagcc aatcagaacc aggaaacgtt acttgaaatg gaaattccat
     9421 tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg ataagcgata gccaaaattt
     9481 ttgtttgaaa ttccccatgt gcatggaatg atccatgccg ccgtgttcgt gatccatgtg
     9541 catcgaatgg tccatttgtt tgctgtttgc tatatctcca tggttatgtc ccatgtgcat
     9601 ggaatgatcc atctgactat ggtcatgttt catctccatc tgtttttcat gagattctgt
     9661 actttctttg tttactactt cttttttatg ctcatgtttt ttctccatat cgttcccatt
     9721 catttttact ccatcccttc tcttactttt tcatgtgtcg atttaagctc agggagttca
     9781 acaatacact gacggaacta aacgccatcg ctcctcctgc aatgattgga ttcaaaaagc
     9841 ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa agcccagaaa agattttgtt
     9901 tgatttttct caatgtcaac ctagacaact cgattgtttg tgcaatggat gccaaactgc
     9961 tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc cgttccactt cccattgcca
    10021 taccgacatc agctaatgct aaggccggtg catcattgat tccatcgcct gccataccga
    10081 cttttttgcc atctttctga agtttttcta cgtaggctgc tttatcctct ggaagtactt
    10141 cagcaaagat atgctttgga tcaataccaa cttgtttacc aatcgtttcg gctgccagtt
    10201 tgttgtctcc tgtcagcata aaaacatcta atcctttatt ctggagctgc ttgattgctt
    10261 gtttggcttc tagtttgatt tgatcagcca cggcaatcca cccgatcact tgcttttcat
    10321 cagctaaaaa catgactgtt tttccctgct gttctaattc taacgcgtgt tcttggtatt
    10381 catcaaatga taggttcaat tcaattaatc gtttacgtgt acccgcaaaa tagcgtactc
    10441 cattaatcgt accgctgatc ccagcacctg ggtgggctgt aaaatcaatc atatcgtatg
    10501 ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt acctagtgga tgttcagaag
    10561 cttgttctaa agtataaaaa atagatagga cttcttttga accgactaga tctgtaactt
    10621 tgggctttcc ttctgtaatc gttccagttt tatccaaaac aatgctgtct aagtgagcag
    10681 ctgcttctaa tgcttctccg cctttaatta aaattccatt tcgtgcacca acacccgttc
    10741 ctaccataat agctgttggg gtcgctaaac ctaatgcaca tggacacgca atgactaata
    10801 cagagacact atggatcaat gcgagttgcc aatcaccagt gatcaaacct gtagctatga
    10861 gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat ccctgagatc cgatctgcga
    10921 ttttttggat tggggctttg ctcccttgag catcttccac catttgaatg atttgcgcta
    10981 atactgtctg cttgccgact tgtgtgactt ccatgtgtag cagaccattc gtattgattg
    11041 tcccgccaaa aagcgtgtca tccggattct tttctacagg aagattttca ccagtaagca
    11101 tactttcatc tatcgcactg cttcctgaaa taattcgacc atctgctgga atctgctcac
    11161 caggacggat gactagttga tcaccaacga ctacttcttc aatcgctaac gtttcttctt
    11221 tcccgtttct tatcacttgg gcggtttttg tttgaagaga catcaattgt ttgattgcat
    11281 tacctgtttt cgtttttgct gtatgttcca aatatttgcc gagcaaaatc agcgtgatga
    11341 tcatactgct gctttcaaaa taaagatctt gggggtgtcc acggaaaaat ccgttgtaaa
    11401 tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa tacatccata tttggtgctt
    11461 tcgtcttgat tgcatggtac gcacctttat aaaaacgagc accgacataa aattggacag
    11521 gagcagacag cactaactgg acgattggaa aatgaaaaaa gtgaacaatc gccgcatggc
    11581 ttcctaacat catagcaatc attgacaaca tcaacggcaa agtcaaaacc gtactcaata
    11641 taagatcgaa tagcattttt ttcaaatacg cttgcttttc ttctgcaatt ttttgtttgt
    11701 gggcttcatc gtacaaaatt gcaccataac cgatgttttc tacgctttgg attaattttt
    11761 cagtggtcgt cccttcatat ttgacacttg ctttttcagt ggccaagttg actgtggcat
    11821 tgaccacacc aggttgtccg ttcagctctt tttcgatcct agcagaacaa tttgcacaag
    11881 tcatacctgt aatgacgaac gtatccattt tggtttctgc tgttgccatc aaatcacctc
    11941 tgctttataa cccaatccat tgattgcttg acaaatcttt tcggattgaa cttttgtttc
    12001 atcaaacttg accgtaccgt ttgctttttt caaatttact ttcacttttt gcacgccgtc
    12061 aagtgcgctt actgtttctt ctactcttgc tgcacaatga ttgcagctca taccttcgat
    12121 tgaaaattgt tgtttcattt tttcttcctc ctattgatgt tgtttacatt cacattgacc
    12181 tgggatacag ttacattcga tcgtatctac tggttgcttt tgttccagtt gtttcaaaat
    12241 ctcattgata tcttcttgag tcaaagtggc ttcttctact agatccgcaa tcgtctctcc
    12301 aatccttttt gcacagatat gggaaaaaag attttccgtc gcactgcgaa ctgtttccat
    12361 ctccgagaca gctggatgat aaatgaattt ttttccttct tgttctgtcc agatcacttc
    12421 tttttttacc aaacgtccca gcaaagtttt gatcgttgct ggtttccaat ccatcgaatc
    12481 agctaagatc tgcgtgattt cttgggctgt cgtctgtcca agtgtccaaa taacacgcat
    12541 gatctcccac tctgaatcac tgatctttac tggcgttttg actttttcca tttatttcac
    12601 acctttcgtt tacaaatgta atcattagct tatgaataca gattacgctt gtaaacaaaa
    12661 actgtcaatt atttttgctc tctattttca atttagttaa aaattcttgt ttctctcgtc
    12721 ttgttttaag gatgatcagt ttatttcttg cattagctac ttggatcagc tgctgtattt
    12781 tcggttcatt gttttcttca aaatgccaga taaaagataa aaattctaag tattccctgt
    12841 ttagtttctc tgaaaatgat tcaggcatat ctggccgaga ttttttgttt atctttcttt
    12901 ttagagaacg ccaaactact cggtagatgg cgagataacg cggcacttta agccaaatga
    12961 tcttatctgc ttctttaatc ctttcctcta atgtagaagt ataatttcct tcaacaatcc
    13021 atgacggatt agataccata aacgattgct gttcttgcaa aaaccacttt tttgcctgaa
    13081 tactgtagtc tgttgtatgc cataaaaggt ctagcggcag tagcggaaag tcggtgattt
    13141 ctttgatctt ccttgcgagg gtcgattttc ccgacccact ttgtccaatc acgatgatct
    13201 tcattcttct ccctcacttc tctttggaat ttggttcgat cataccactt ataacgccgc
    13261 aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc cttgtttcga ataaatggtg
    13321 ttcaatcagc tgaccttcag cattaactca aaaaacggca aaatatgagg agctattttt
    13381 tcgtttgttc ctctaatact caggtcaaat cgctgatttt acaacctctt ccttaaacgg
    13441 tgttctcctg ccaacacctc ggtcacaagg cacaatagtc aacaaacatg agaagttgtt
    13501 ttttgaaatt gctccttgtg actcggagtt aaacggcagg ctcactacct ctttcttaaa
    13561 cggtgttcaa aagcttgcat ctgcggtaat aagcccagca aaacgaaaaa tatgaggggc
    13621 tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcaca acctctgatt
    13681 cacggtgttc cagaagtagc ttctgcgaaa aacaagccga aacttcacaa aaatttgaga
    13741 agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctctt
    13801 gtaacaagta tttctatttt aaggctgaac cactgtttct ggttcagtga tttctttgtt
    13861 tttgtctcga cgagcaattg ctggcaagat catacctaat aagatcaaga caattggtgt
    13921 gatgatattc gtgaataatt ggaaccacca tgctttagga tcagcagtat aatctacttt
    13981 tggcaccatt cctaggatac atgcaaacgc agtgaaggca aagcaccaag caccaaagat
    14041 aaaaccgatt tttggatttt taacaaattt atattcagaa ttgaatttct tataagcttt
    14101 attcaacatc atatatgcca agaacaccca taggtaacgc attggcatga caactgagtt
    14161 caagttcgtc aaccatttta ctaattcatt catatctttg ataccaaaga tcggcaacaa
    14221 aataatgatt cctactagta tacctgtcag aagataacca ttcaccaatg tacctttacg
    14281 attacgttta cgtagccagt ttggtacaaa ttctggatcc gcatctgcta acaagatttt
    14341 tagtggtgca tcaattgaaa aggctaatgc ggagatttgt ccaatcatgt ttgtcaatgc
    14401 gtaaatgatc atcaatgagt taccgatacc atagtaatta cctaaaattt gaaaagcact
    14461 gtatgcccca tttgccatca agtctttagg aatattatcg ctagcaaaca gcatacccat
    14521 tgcgactgag cctaatacag cacaaatccc taccattgct gctaagaaga tcatcccttt
    14581 aggaaattcc tttgcaggat ttcttgtaga gttgacataa ggggatattt tttccgctcc
    14641 tccaactgca aatactagca tcgaaacggt tgtgaaataa ctgaaatcaa atgttggaat
    14701 atatgttttc acttgattca tatgtggtgt ggcaaattcc atcgttggat tgatgaacgg
    14761 tgccgttaca gctaaaagaa taaataagat cgacatgacg aacatcgctg ttccggccag
    14821 tccgccaatc actttcaaag tagaaagtcc ttttgttgat aaatataaaa aggcaagaaa
    14881 tataataagt gaaataactg ctaccatttg tactgacatc gtatttacga tatttccatt
    14941 tccttggact gcccaaccgc cagcaatcag gattgcttga ggcttttgtg ccaaataagg
    15001 aatatggacg acccagtaag tccaagcggc atagtatgcc agtcgtttat tagatgtcgt
    15061 attttcgacc catgaactga cgcctccttt actatccttg aaggctgacc ctaattgtcc
    15121 aacgatcaat gcgtatggaa taaaataaat ggccaaaatc aatagccaag aggtgacaac
    15181 tgatactcct tgctgagcat agttattaac tacattcccc atgccccaaa ccatgttaaa
    15241 tgcaattaac gcaacagtaa accagcgtaa ttgtttgttg ttcattcgtt ctctccttta
    15301 tgataaaaat ttgttatcca gatgtaatca tattcaattt ttaacaaact tacaagacag
    15361 tgttatttta tgtgtgtatt cttttattta atacacatcg ttttttcata ttttttccat
    15421 caaaaaaata agttattttc ttttatttca tttaccttat tgaatttcaa gctattttga
    15481 attttctgca aaattaaaaa cattagttat tttataatcc tatctatttt ctttacattt
    15541 ttcattaaga aaaggatata aaaaaaagag gacgttcatt cgtcctcttc attgatcaac
    15601 gtttattttt tacatgcaag cttcgcatgg cattcaaaac agctaatact gtcaccccaa
    15661 catctgcaaa tactgcatct cccattgatg caaaaccaaa agcacctaag gcaagtacca
    15721 aaatttttat cccaatagca aagacaatgt tctgtttgac gattttcaat gttttctttg
    15781 ctaaatggat tgcttctgca atcttcgatg gttgatcatt catgatgact acatctgctg
    15841 cttcaattgc agcatcgctt cccaatccac ccattgcgat tcctacatct gctcttgcta
    15901 gcaccggtgc atcgttgatt ccatctccga caaatgcggt ctttttattg tttctttgta
    15961 agatttcctc taaccgctgc actttatctt ctggaagcaa ttcgctatat atcttgtcca
    16021 cgcctacttg cttcccgata tgatcagcaa tttttttcga atcgcccgtc aacatgactg
    16081 tgttcttcac accagccttt tttaattgaa ccaatgcgtc tacggcatct tctttcaagg
    16141 tatctgcaat aacaagataa ccactgtaac tttgatcgat cgctagataa agcaatgtac
    16201 cgatttcttg acttgaagga aaagagattt caaatctttc catcaatttg tgattacctg
    16261 ctaatacttg tttgccatca acttcagcta agatcccttc acctgcaaat tcttggatat
    16321 tagctgttgg taaaagcgga cctgcgtagc cttctaaaac agattgagca atcggatgtg
    16381 tagaaaactg ttctgcactc gcaacatatt gcatgaatat ctttggatcg atttttgtat
    16441 cgatcgtctg gatcgaaaag tcacctttcg ttaatgtgcc ggttttatcg aataccactg
    16501 tctcagtttg tgcaagcaac tccaagtaat ttgaaccttt tactaataca ccgattttac
    16561 ttgctcctcc gataccgcca aagaaactaa ggggtacgga gatcactaat gcacatggac
    16621 atgagatcac taaaaatgtc aatgctcgat agatccattc agaaaacgcc gctccagaaa
    16681 caacaagtgg aggtaaaaca gctaataaaa cggccaatcc tacaacgact ggtgtgtaat
    16741 agcgcgcgaa tttagtaatg aaattttcag ctggtgcttt tttactgctg gcattttcta
    16801 ctaattctaa taatttgctg atagtagaat tttcataaga tttttctact tgaactaaaa
    16861 atggtttatt tttattgata aatccactta atactgtttc accaacatat acagatttag
    16921 ggactgattc tcctgtaagt gctgacgtat cgactaatga ttctccttcg atgatcgttc
    16981 catctaacgg tacacgttca ccaggcttca caagtacaaa ctgaccgatc tgcacgtctt
    17041 cagggttcac ggtcatcaat ccttccgtcg tctgcacgtg agctgtttct ggacggatcg
    17101 ccatcaattc gcgaatcgat tttcttgaat gactgactgc caatccttgg aaatactcgc
    17161 cgatttgata aaatagcatc actgctacgg cttctggata ttcaccgatc aacatagcgc
    17221 caatcgtagc aacagacata agaaaattct catcaaagat ttttcctttg acgatattca
    17281 tcactgcctt cttcactaca tcatatccta tcagcagata aatcaataaa taccctatca
    17341 agcgaattgg ttcattaaaa tcaaataata gcaataaagc gatacctgca agggaaaata
    17401 aaatccggaa cagcgtattt ctctcttttt catttccatg ttcagatact ttttccttat
    17461 ctatttctgt tactttgaca tctggttcta attccttcac tacttttttt atttcttgtt
    17521 caatcgtagc caaatgatct tcctctgcgt caatcgttag ttttccagaa gtgaagttta
    17581 ccgttgcttc cttgactcca ttgatcttct gaactccttt ttcgattttc attgcacagt
    17641 tcgcacaatc taaaccttct aatctgtaac ttttcatttt aatgacctcc tcgcctcaac
    17701 atatgaatat ctcttcataa ctaattataa atgaacggtt attcatatgt caaggtatcc
    17761 gaaagaaaaa atgaaaaaca tctgcatcgg aaaatttctt ccatacagat gttttggctt
    17821 attctttctt tttcttttcg atcattgcca ctgctcttat ccaacccgca cttgccttct
    17881 tgatcttgac aggatagcaa ctaagtttga agcctgttgc tggcaatttg tctaaattca
    17941 ttaatttttc gataggacaa tatctttttt caatccctgc aaaatgtcct tcccagatat
    18001 tcgaagctcc actacgcttt caaaaatata gaaagagttc tttaatgaaa attatttcaa
    18061 ctttccataa acagtaaccc catataaata aaagacgtaa aagtcacatg gaagaactta
    18121 tacaggagaa cgcatagagt caggtgaaat acatgtatcc cacctgactc tatgctttat
    18181 ctaaccttta ttattctctt ttatacaatt tctgcaccga atggcatcaa cgccaatcga
    18241 gcaagtttga aagattgctt ggcaaaaggt atccctatga tcgtgataca tagaaggatg
    18301 ccgctgacga catgtgcgag agcaattggt aggcctgaaa cgatcagcca gatgatatta
    18361 acaatcaatg acatgccaga ctggtgataa attacttctt tgccaaaagg cgcaaaagaa
    18421 agggaggcga tcttaaaaca ttgcataccg attgggatac ctatgatcgt gatacaccaa
    18481 agtatccccg ctgctaacca gctaagacca ccgattagtc cgccgaatat aaaccagatg
    18541 acatttccta aacaactcat tgtatttctc cttctattac tgtttttgat taaaactggg
    18601 caaaaaatga ttttattctt ctttttttag cttccagtat caaatattct gcaaattttt
    18661 tttggatttc ttccgtattt tcttttgtta ctaatgtgtg ttggattagt tttcctgatg
    18721 gtcgctgatc caatcgttct tctgactgtt gcacgtccgc ttgcgcaaat ccgataatcc
    18781 gtttttcttt tgttacagca acgagcaata gtttattctt atcatttagg tcagctgccg
    18841 tttttgcttt tacgaatgat tctggatgat cagtaatcag ctgattgatt tgaggtagat
    18901 cttcttttac tgcccaccgt atttccatca tttctcactt ttctttcgct catttttatc
    18961 tccactaaaa aaatcgactg cggcaattgc atctatccag ctttgtgcaa tgatctcatg
    19021 tccgccaatc gttgggtgga ccccatcttc tcttgtatat ttcgaaaaac cataaacaat
    19081 ccctaaagca ttgaatatac catctaaagg aaccagcaat gcgccatatt ccttagcaag
    19141 tttacgaaca atttggattt tagggtctaa gtctttacgc cagccatgac ggtcgtcggt
    19201 tataggaagt acaaacggct ccatcaagat gatgttgcag attcccactg tttgaagttc
    19261 atttagtaat tttcgataaa cggattcaaa ttgctgagca gatttctcag ttccgaatat
    19321 agcctgatcg ctagtattat gccaggtatc attgattccg atcaaaatcg atacatagtc
    19381 aggttgtaaa gctaaacaat cttcttgcca gcgtacttgc agatctgcaa ttttgtctcc
    19441 tcctatccca cggttcatgc attcaaattc catcgttgga tattgggaga ttagttttcc
    19501 ggcaatcaaa agtggataac ctttacctag atcagttgct tctgctcggt ctcttcctgc
    19561 gtctgtaatg ctatcaccga taaataatat acgttttttc atttgtcaaa aactcctttt
    19621 tgaataaaac gatgattttc ttccttatat tatcccataa gccctatcaa gacgaaagag
    19681 aaaatatatg tataaaaaat gacaccagcc tagtcccttc aaacaattga atgaacgctc
    19741 atcaagtgtc tagtttttta tgctattttt ttcttaacgt gccggattcc ccttgattcc
    19801 ttcgttgttt ggtccagaga gatcgatcac acctaagctt ccgtcaatca gaccactgat
    19861 gacgccatca gaacatatat ttataggctc atctaagact tctagtaaat gtccctttac
    19921 taatatctct tctagattgc ttgggataga ttggtcataa ctttatctgg tccgatgatt
    19981 tcaagggtga attgaactta catatggatt attgcatgct gaaaatacgg acctactcca
    20041 tcttcgaatg ttactattag tttgttttca ggtgctagat gctgctttat ttcaacagca
    20101 gaagcatcat caaatcagat ttccatttga attaccttct ttgtaaatgt ttgattgatt
    20161 gcgatttcgt tatagaacct tgtgaatcaa atagcaattt cttttgcttt cttctcttgt
    20221 ctgctggaaa cttcctttat cccgtatttt aaaaaaagaa ctgcgagagt tcgcagttct
    20281 tttttagaat aaacggtgct ctccggccaa ctcctcggtc acaagtcaca atagtcatca
    20341 aacatgaaaa actgtttttt gaaattactc cttgtgactc ggagctgaac ggcaggcgca
    20401 caacctcttc cttaaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatgc
    20461 aaaaatgtgg aaagccattt ccacaaaatt tttcttaata ctcgaaacta aacagctttt
    20521 tcacaacctc tccactaaac ggtattcaaa agcttacatc tgcggtaata agtcccgcca
    20581 attcaaaaac atgaacagct gtttttgctt ggctgttcta attacttgat gcagaacagc
    20641 tttttcatca cctcttatgg ataaattcgg ttcaacaaac gtgggaatgg aatagcttca
    20701 cgtacatggt cgataccagc aatccaagtg actgttcgtt caagacctag accaaaacca
    20761 gagtgcggaa cagaaccgta tttacgtaga tcaagatacc atgaatattc tttttcatcc
    20821 aaaccgtgat tacggatttg ttctagcaag aaatcatagt ctgttgcccg ttcgcttccg
    20881 ccaataattt caccgtaacc ttctggtgcg atcatatctg cacagatcac cacgtcttct
    20941 cttgttggat gtggtttcat gtagaatggc ttgatggctt ttggatagtt taggataaag
    21001 acaggctgtt cgaatgaatt cgcaatgaac gtttcatgag gtgaaccaaa gtcttcgccc
    21061 cattcgatgt catcaaatcc atttttcttc agtaattcga tcgcatcatc ataagtaata
    21121 cgagggaatg gcaattttgt ataagttttt aagacttctt tgtctcgtcc tagtacatct
    21181 aatgcatatt cgcaatgatc taaaacattt tgtactaaga atgctacata ttgttcctgt
    21241 acttctaaac tttcttcttg atgagtgaaa gccatttctg gttcgatcat ccagaattcg
    21301 atcaaatgac gtcgtgtttt tgatttttca gcacggaaag tcggaccgaa tgaaaagact
    21361 tttccgaaag ccatagcagc agcttccatg tataattggc cactttgaga taaataagct
    21421 ttttgatcaa agtaatttgt ttcgaataga tccgtagtgc cttctggtgc agaaccagtc
    21481 aagatcggcg gatcaatttt gacaaaatga tgttgattaa agaattcata cgttgcacga
    21541 atgatttcat ttctgatctg cataatcgca tgttgtcttg aagaacgcag ccataaatga
    21601 cgatgatcca tcaagaagtc tgttccgtgt tcttttggtg tgatcggata atcgtggctc
    21661 tcgcttacca cttccacatt tgtaattccg atttcataac caaattttga acgactgtct
    21721 tcacggattt ctccagttac taacacggat gtttcttggt gtagattttt agctgtttgg
    21781 aaaacttctt ccgacacttc gctttttaca actacacctt ggaaaaaggc tgttccgtct
    21841 cttaattgta aaaatgcgat ttttccgctt gatcgcttat ttgcgaccca agcgccgatt
    21901 gttactgttt ctcccacatg gtttttcgaa tcgatgattt gaatttgttc cacgtatgac
    21961 tctcctttta tattccttga atttctttag gattaatttt gacttttact ctcaataaat
    22021 tgcttgattc tgcgtaccgc ttcttctaac gtagcgcgat cagtagcata actcatgcgg
    22081 acattttctg gtgccccaaa gccttcgcca gtcacaagcg ctacccctgt ttcttctaaa
    22141 agatcttcta cccattgtgt gacattttca tatttacaca tgcgtagcgt ttcttttaca
    22201 ttaggaaaaa gataaaaagc tccttgtggc ttttcaagag aaacacccgg cagttctgcc
    22261 acaagcgaat ataacgtatt caggcgttct tcaaaggcgt tccgcatttc ttccactgta
    22321 tcttgctcgc ctttcaatgc ttcaacagct gcatattgac tgactgctgt cggattactg
    22381 gttgactggg aagcaacagc gatcattcca ttgatgatca caggatttcc caccgcataa
    22441 ccaatgcgcc agcctgtcat cgcatatgtt ttagacacgc cgttgatgat gatagtctga
    22501 tttttgattg cttctgaaat cgttgcaatt ggagtaaact cattgccatt ataaactaaa
    22561 cgaccataaa tatcatcagc aactatcaaa atatcatttt ttactgccca ttcaccgatt
    22621 gcctgcagtt cattcttgct ataaatcatt ccagtcggat tcgatggaga attgatgatt
    22681 acggcttttg ttttttctga acgtgcttgt tctagctgtt caacagtaac tttccagcta
    22741 ttggattctt cccctttcac aaagacagga agtccttctg ccagttttac ttgctcaccg
    22801 taactgaccc agtaaggcac aggaatgatg acttcatctt gcgggtcaag gatcgtttgg
    22861 aaaagggtat acaaagcaaa tttcgctccg tctgttacga ctgtttgtga aggttcatag
    22921 acaagtccat agtttttctt cagataatca acgattgcct gtcgaagctc tgggattcct
    22981 gctgttggtg tataatagct agctttcccg ttgcggattg cttcgattgc tgcttcttgg
    23041 atgttttctg gtgtcgcaaa atccggttct cccacagtca aactcagcac atctctgcct
    23101 tttgctttta atgccttggc ctttgccgca gctgccaaag tgacagacgg ttctaatcgt
    23161 tttgttcttt ttgataactc catcccattc attcctttac cttttaaatt cccttgattg
    23221 tcttgacttc atctccagat tcaaagccaa tcaggtaata attcagtttc ccatttgttt
    23281 cttttcctac tacttcccac acaggctgat catcatacat ccctaaagca gctttttcga
    23341 tttggatttc aggatgggcc gcagcaattg tctgttttgc ttcgttttca gtcagtccct
    23401 ttgtttgatc caatactttt actttttcac cagatttagg aatgataacg gcgatttctt
    23461 ttcctttatc atttgtacct gtcagtgaaa agtatgtctt ctttcgagta aaccaataaa
    23521 aatgatcggc ttcctcaaga tttgcatatt tctttgcgat ttctactgct tctttcttcg
    23581 cctgcaccgc cggacgactg gaacgcaagt aaaaaatcga gctgatcacg atgaccgcta
    23641 ataaaaatac gatcaagcca agtaacacgc gattaatcgt tttttccttt gtctgttttt
    23701 tcttcaaggg tcgtcgctcc tttatcttca ttccttttca ttatagcaga ttttaattat
    23761 gaaaaaagac agctcctcta acttccttct tcatttttct ttaagaattc atggatgtcc
    23821 tgatagattt cctccatagc tagctctttg atcggtactt ttttcggtaa tgcatggatg
    23881 atccgtgcac catattttgc agtgactagt cttcgatcca aaataagcaa tacgccacga
    23941 tctgtctccg tccttatcaa gcgacctaat gcttggcgta acttcaaagc tgctttaggt
    24001 acggattcct ggtaaaaagg attgatcccc tctgattcta aataagcgtt cctagctttg
    24061 accatcgggc gaagcggatt atcgaagggt aaacgtgtga ctacaacgat ctgcaaagca
    24121 tccccaggca aatctacacc ttcccaaaag ctgtcagcgc caaacaatac gcttttttct
    24181 gacaaaatga agcgtttcaa aagcttctcc cggcttccgc caaacccttg agctagtatt
    24241 tctcgtcctt tttctagcat agacagatgc attttctgat agaccttctg caaaacatca
    24301 tgagaagtga ataacacaag tattggccga tcttcttgtt cgatcagacc tgtaagtgct
    24361 gtctctaaat aatcgatata tgcttgattg ctgacttgat ggacggcagg tgcctctttt
    24421 ggcacataga tccttgcctg tttctcataa tcataaggag aggcaatcac tttaagaggt
    24481 gctgcttcaa tgccaagccg cttagagaga tagtgtcgat tagggccgat cttcagcgtt
    24541 cctccaatat aaagaatacg atcatatcga ggataccaaa cagtcgacgg caacatagcc
    24601 gcttgaaaat catggagatg aaaaatcatc tgctgcgctg tgttcccagg aacgaaccaa
    24661 tggatcaaat gatctcgcca gtcttccagc cattgatcca tcagcaagta ctgcttgtct
    24721 aattgatcaa ataagctata taaacttgct aaatcgatcc gcatctggtt tgtccagctt
    24781 tcttggataa tctcaaaagt cagacgcatt tgtgactgta gctgctggat ctcctgataa
    24841 aaaagctgca ggtgatcaat ggctgtccca gattccagtg gcagctgatc caacatctct
    24901 tttgtcatca tgacttcttc tggatactgt ccgttaggtt gcttgccgct cactaaactc
    24961 attaatcctt ctgccataca aatctgtgct tcgcatagcg cagaaagttc ttctcgataa
    25021 atcgataacc agcgtaaggc ttcatgttct ttcggtaaat agctttgtat tcggtcaaac
    25081 agctgatttg gttcttctaa ttgctggatt tgtttatgga aatagttaat acttaatcgt
    25141 ttgtcattta ctttttcagc gatttccggc aaatgatgta cttcatcgat caacaaaaat
    25201 ggactagctg gaagcaatgg ttcttttcgc tgtgtttctt gtattaaaaa cgcatgattg
    25261 acaatcaaaa aattactttg cctgattttc ttttgcaaga aacgaacaaa gtcttgttcg
    25321 taaaatgact gatttcttgc taaaaatcca atacctctgt gcgcgatatc tgcaaagaat
    25381 gaatggttca aatttgttaa atgtaattca tccaaatcac cggtcttcgt ttgcgtcagc
    25441 caaactaaga ccgccatttg atacaaagca tattgttttt gttctggcgg ttccacaagt
    25501 gtgcctttaa aacgctgtaa atcaatatag tggcgatagc ttttgataat tacaggttgt
    25561 atgggctgat ctagcaattg attcagcaat ggaatgtctt tttctatgat ctgctgctgc
    25621 aaaactagcg aaacggtact gatgacagcg ggtttttcag gtgtagccaa ataactcaat
    25681 ggcagcagat aaccgattgt tttacccatt cctgtagctg cttcgatcat caaatctttt
    25741 gattcatctt ttgtgaaatg gtcgtagacc gcattcatca gtctgttctg ttcttttcga
    25801 tacatcagtg ttttaccaaa cattttttct tttgcttgtt tttttcttgg atatgtccgc
    25861 tcgccataat ggacggatgt gaatagttcc acttcttttt tctgcaaagc tagcccatcg
    25921 acgatttcta atgagggatc aagtggctct ggtttttctt tcatttcttc aatcacatga
    25981 taaatgaaac gactcgtatc catacctgtg tgtctactta atcgcgcaat ctgctctaac
    26041 gtgatgattg gcaattttct catccgttct tcgatcagca aaagaagttg acccgtcact
    26101 tcggcatcac tatctgcctg atgaggattc tcatgaataa atccaaggct ttctgacaaa
    26161 tcagctaagc gaaagcttgg ttccgtcggc aagaaaattt gtgcaagttc tactgtatcg
    26221 atgcctggta tcttcaaact aggaagtccg catcgcatga gttcatggtt caaaaaatta
    26281 taatcaaaat aaatattatg cgcgacaaat atggtgtctg ccaaaagatt gtaaatcgtt
    26341 tgggcgacat cttcgaaata aggcgctttt tggacgcgtt gattcgtgat ccctgttaaa
    26401 tgctggattt gttttgaaat cttccgccct ggatgaatat cgatcgaaaa acgtgaagtg
    26461 atccgacctg attcaatcat gacgcagcca aattggatga tccgatcttc ttttggattc
    26521 gtcccagtcg tttcgatatc aacgactgca taagttcttg gtttcaaaat atcttcctct
    26581 tctcctgcct ctaatgttct tttgaaatta tactacaaag tgcagaaaaa tactctcatc
    26641 gctttttaaa gaaaatagac aaaaacgccc agacacgcaa atgtctgggc gtttattttt
    26701 tagtataggt taattaagct ttagtaacgt ttgttgcttg aggtccgcgt tggccttctt
    26761 caacatcgaa agttactgct tggccttctt ctaaagtttt gaatccgtcg ccttggatag
    26821 ctgagaaatg aacgaataca tcgtttccgt tttctgcagt gatgaatcca aatccttttt
    26881 ctgagttaaa ccattttact gtacctgttt ccataataaa aatcctcctt gtgtatcaac
    26941 acatattttg caattacttg aatggtgtat atctggaaga tgtttatttg aaacaagcta
    27001 ccacagatta ccgaacaaaa actactccta tagtatatca taccgtttac aaaaaagcta
    27061 gagtttttcg acttttttta ttaaaaaaag ttttctcagc gattacataa agtgaaaaac
    27121 cgaacttttt ttaaaataaa cgacatattg ttcagttggt gtttttttgc tctgatgttc
    27181 ttctttaaac taaaaaaatt attctttctg actacttatc ggttctgttt caacggtcga
    27241 ggctggggaa gattctttag taggtctgat gttctcatgg tatttctgat agcctatgac
    27301 agttaccaga agaacaaggc aaaccaatac gatcaacgag attagttttt cttttttgct
    27361 catatctata cctcataaat aacaggatga tgtccatcat atttctgagt atgagtaaaa
    27421 gcaaaagctg cagctgtaga atgtaagaaa tgccagcaac tttcataatc acaggtcgga
    27481 cagtaccaaa agtaaccaaa tcttggctgc aaagaccgtt cttctgtttc aggaatcgca
    27541 gaatcaagat ctgttgtctg tacataaaca tttttgtaca gacgtgtgcc tgtaatagga
    27601 gaccgctgtg tttcttcagc aaaaactggc tgacttgtta aacttaggga tagaaacagc
    27661 aaacaccata acagtctttt tttcataaag acctccttat cgataagttt ttcccaatta
    27721 aaatcatatc tcaagaacgc ccgttattca cattttgttc ataaatgatg ttttttgtat
    27781 cataatcgtc aaaaagaggt cgtgaccacg tcacaacctc ttttt
//
LOCUS       DAZUMM010000030        27758 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000030 DAZUMM010000000
VERSION     DAZUMM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.030"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(304..1248)
                     /locus_tag="ACG8E6_001255"
     CDS             complement(304..1248)
                     /locus_tag="ACG8E6_001255"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS5061682.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1544..2488)
                     /locus_tag="ACG8E6_001256"
     CDS             complement(1544..2488)
                     /locus_tag="ACG8E6_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS5061683.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2709..3923
                     /locus_tag="ACG8E6_001257"
     CDS             2709..3923
                     /locus_tag="ACG8E6_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5061684.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3945..5960
                     /locus_tag="ACG8E6_001258"
     CDS             3945..5960
                     /locus_tag="ACG8E6_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HFS5061685.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6043..7416)
                     /locus_tag="ACG8E6_001259"
     CDS             complement(6043..7416)
                     /locus_tag="ACG8E6_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS5061686.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7442..7726)
                     /locus_tag="ACG8E6_001260"
     CDS             complement(7442..7726)
                     /locus_tag="ACG8E6_001260"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5061687.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7728..9743)
                     /locus_tag="ACG8E6_001261"
     CDS             complement(7728..9743)
                     /locus_tag="ACG8E6_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5061688.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIKKGDE"
     gene            complement(9922..10227)
                     /locus_tag="ACG8E6_001262"
     CDS             complement(9922..10227)
                     /locus_tag="ACG8E6_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFS5061689.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10316..11347)
                     /locus_tag="ACG8E6_001263"
     CDS             complement(10316..11347)
                     /locus_tag="ACG8E6_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5061690.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11554..12429
                     /locus_tag="ACG8E6_001264"
     CDS             11554..12429
                     /locus_tag="ACG8E6_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5061691.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12581..13423)
                     /locus_tag="ACG8E6_001265"
     CDS             complement(12581..13423)
                     /locus_tag="ACG8E6_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5061692.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13515..15392)
                     /locus_tag="ACG8E6_001266"
     CDS             complement(13515..15392)
                     /locus_tag="ACG8E6_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5061693.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     RPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEATIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIDNQTNTPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDHETSGTYAYVLYDG
                     DKEIDPTNYSVTEQTNGFTVSVDSNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15389..16810)
                     /locus_tag="ACG8E6_001267"
     CDS             complement(15389..16810)
                     /locus_tag="ACG8E6_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5061694.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16813..20202)
                     /locus_tag="ACG8E6_001268"
     CDS             complement(16813..20202)
                     /locus_tag="ACG8E6_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5061695.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20459..21850
                     /locus_tag="ACG8E6_001269"
     CDS             20459..21850
                     /locus_tag="ACG8E6_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5061696.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21923..22570)
                     /locus_tag="ACG8E6_001270"
     CDS             complement(21923..22570)
                     /locus_tag="ACG8E6_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5061697.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22578..23264)
                     /locus_tag="ACG8E6_001271"
     CDS             complement(22578..23264)
                     /locus_tag="ACG8E6_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5061698.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23270..24343)
                     /locus_tag="ACG8E6_001272"
     CDS             complement(23270..24343)
                     /locus_tag="ACG8E6_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5061699.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24565..25239
                     /locus_tag="ACG8E6_001273"
     CDS             24565..25239
                     /locus_tag="ACG8E6_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5061700.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25244..26584
                     /locus_tag="ACG8E6_001274"
     CDS             25244..26584
                     /locus_tag="ACG8E6_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS5061701.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26733..27719)
                     /locus_tag="ACG8E6_001275"
     CDS             complement(26733..27719)
                     /locus_tag="ACG8E6_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFS5061702.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8092 a         5256 c         5036 g         9374 t
ORIGIN      
        1 ttgaatgata aaaaccagtt tctttattca gttaatcaaa tatgaacgaa ttagatatga
       61 atcattgtat ttgccgttgc ggaaggattt tttaattaac cacagataaa cattgataac
      121 gattcgctta ttgtaatcaa cagattaaat ttcatatgtt catatgtacg gatacatttt
      181 cggaccgatt aattttaacc attctgttta tttgcttacc tattagatat caataaaaaa
      241 ctgcttaaca gctatttcag ctgttaagca gttttaggat ttttagttca tattcattgt
      301 ttcttataat acagtgttca atacttctct taaagcactt gttgatttgc tgaacattgc
      361 tttttcttct tcgttgattg ataattcaac aacttcacga acaccatttt cgtcaacgat
      421 tgatggcaca cctgtaaaga tatctttttc gtcgtattca ccagttaaat aagcagatac
      481 aggcaataca gcttgttcgt tattcaagat agcttttacg atacgtgcag tactcatacc
      541 aataccatag taagttgctt ttttacgatc aatgatctca taagcagcat tacggacttt
      601 atcagcaatc acatctaatt catcttgggc aatacgatgg tctttttcta caatttcaaa
      661 cactggttta ccaccgacag ttgtatgtga ccatgcagca acttctgaat caccatgttc
      721 acctaagata tagccgtgga cgctgcgtgg gtcaactttt aatttcaatg cgatttcttt
      781 acggaaacga gttgtatcca atgttgttcc agtaccgata acacgtgatg ttggcagacc
      841 agattcgttc caagcgatat atgtcaaaat atctactggg tttgaagcaa caacgataat
      901 accgtcgaat cctgatccca tgatttcttt tacgatttgg cgcatgattg acgcatttgt
      961 tttaactaaa tctaaacgag attgaccagg tttttggttg ataccagctg tgataacaac
     1021 gatatcagca tctttacatt cttcatagcc acctgaccat acagccacat tttcatcgcc
     1081 ccaagccata ccatctaaaa ggtccaatgc ttccccttct gctttttctt gattcacatc
     1141 gatcaatact aattcattag agattccttg gttgatcatt gcataagcga tacttgttcc
     1201 aacaaaacct gtaccgacga ttactacttt acgacttgtt tttttcatga ataaacagct
     1261 ccttttcata actatttgtt actttcatga aaagtatagt cttgtttatt cattttatca
     1321 acatgttaat tataatagtt gttattataa tttttgatgg aaacgaattc ctgaatacgc
     1381 ataacagata aatgacaaat gtttacacct atatcccctg ataatacaac caaaagttta
     1441 gaactgaaaa taaatccaaa aaaattcgct gcaacgatat aataagacag caatcgcacc
     1501 taaaacaatt ttccttcata tgctttagtg aaagattttc ttcttaatgt ttatttctcc
     1561 attcattggg agtacatgtt gaatacttct tgaataattt gaataaagaa gtcgtttgtt
     1621 tatatccgat acgattagcg atttcttcca acgaaaaact cgtttcttga atcagttcca
     1681 acgcaaagtc gaattttttc tttagaacca gttcttgaaa ggaagcatca aaatacattt
     1741 tcaatttatt actaagatag accggattgt agccaaaatg agctgctgtt tcttttaacg
     1801 tacaagaagt aaaatgctta tcgatataat ctaatactct aagcatctca ttttctgcgc
     1861 atccatctgc tgatgaaaag ctttgttgtt ctaataaatg agtcaattcg atcagtaata
     1921 gtgatgtata gagatttaac gaacggatct tatgactgta tgtatcaaaa taaatcaaca
     1981 gcatattttc aatcaaatgc gtcaaacgac ttgtttttcc gccgcgaaaa tgaatgaatt
     2041 gtgtatgttc ccctttttct tttgatgcat ttatcaaaaa agaagaaagt agattatttg
     2101 tgcttgccaa atgattcaat atctctgtag ataaggtttc ttcttttaat aagatgttga
     2161 ttaaaatatc ctcttgccct gtgtattcga ttctttgagt agcttcttta tcaatgatca
     2221 ccacgtcact ttcttccatc tctatccttt ctccattaat aatttgtaaa catgtaccgc
     2281 tatacatata attgatttca acaaaatcat gtgtatgctc gggaacttcc gcatatcgac
     2341 ggtgtttact gatatgaata tttccctgtt gaaatagatc ccctcggtgg atttttggcg
     2401 gtgtctgaag atggtaagct aagtttaaat cattgatatt cattccttgt tcacgctgca
     2461 gagtttcgat tgattcttcg ttacgcaaaa aaaatgtcta attcagctag ttccaattga
     2521 ctccctcctt tttgtaagcg gtttctttcg cttattagtt cacgctgtac tgctattata
     2581 tgcaaagcta tttttggttt cataaagact ttctatggtt gggacatgaa cttcctgtga
     2641 ttccgctatt gagagaataa ccacttcata ctatattttt gataagcgct tacaaaaatg
     2701 aaaggaacat gcttatgaat aaaaagatga tgaaattttc tctgctgtca gtctctctta
     2761 ttttgacttc tgctggtgca gtcacgtctg caattcccag tatgtcagct acttatgcca
     2821 cgcgttcttt atctgctgtt gaattattaa cgactgctcc ctcttttatg gtcactattt
     2881 ttgttttgat tagtagtttt attgctagga aaatcggaca aaaacaaact gtgatcatcg
     2941 gattagtgat tgcatcgatt tatggtatcc tccccctctt ctcacaaagt tacagtattg
     3001 tcttagcttc aagagctgga ctaggtattg gctttggatt gatcaattct ttggcagtca
     3061 gcatgattgc tgccttttat cacggagatg aacgagcctc tatgattggt tttcaaagtg
     3121 catttcaagg atttggtgca gctttaatga catttgttgc aggacagcta gtacagttcg
     3181 gttggcaata taccttttta gtctacgcca tcagcttacc tattcttgtc ttgtttgttt
     3241 tgtttgttcc tacacctgaa acaactgaac ttcaatcaca tggcacagcc ccaacaagac
     3301 aagtaatcaa aacgaatcct aaaatgatta gttatggtat ttttctcttg ctggtcatcg
     3361 tggtatataa tgctgtatcg attaaattag ccacagtcct gacgacttat tctctaggaa
     3421 acgaaactaa tgcagcgaat atcttaacaa tcatgcaatt ggcaagtatg gctgccggag
     3481 tttgttttgg ctggcttcag acaaaagcac atcgctatat gcttacactt tctttactac
     3541 tgatgggaat cggctttgta ttgattgcca ctgtgataaa cctgtatgtg attgggtttg
     3601 gtgcgatcct aacaggaatt tctttttcgt tattcattcc ttacttattc aaccaagtaa
     3661 gtatccaagc gcccaagtac gcggagagtt tcaatacttc aatcttattg gttggcgcta
     3721 atttaggtac tttcattgca ccatatggtt tgctctttct ctcttttgct tctcttggga
     3781 gtaaaacact tgctgttttc gtcaatggca gtatcttgct gttgattagc gcgcttatta
     3841 gccttctagt agtacacagg acgaatagaa agacagtgat aagagtagat gaagaaataa
     3901 aagaacaatt gacaacaaaa taaatttgat ggaggtcgtg taaaatgaca aatgaaaaac
     3961 ataaagatag atggctttgg tatcctggag actttgagat ccgccatgga ctgctacaaa
     4021 atttccaacg ggaagaacga ggttttgact ggccagcgta ctggtacatg gatgattgcc
     4081 atcggaacgt caagtttaag agatattact ttcttgatca accttccatg tttaaagtga
     4141 cgatccaagg agttgggtat gtagaaatta atggacaaaa acatccatgt ggaaaatggc
     4201 tgacttgtcc agctggtaaa gcaaaaattc gtatctttgt aggacataca tcagggctac
     4261 cagcgatgtt tatcgaggga gacgaagtaa aatcagatat tggctggaca gcaagtaatt
     4321 ttattgaaga atatccagct ggctggagtc cgctttatgt cgatatcgca aaagatccaa
     4381 atcaaatcta ttatcaaaaa gaatgcgtcc atccagtcac tgaaacggag attggcggag
     4441 gaatcttatt cgattttggg cgagcagtga atggctgtgt agaactaata tctctggatg
     4501 accatatcga atctatcact ctatgttatg gagagtccga tgtagaagct atggatatcg
     4561 attattgcta ctataagcaa ataggaatga caagaaaaat gaaagctcgt aaacgcgctt
     4621 ttcgctattt gtttatacca gaggtttcta ctggaatggt tcaacttcaa gtttttcatg
     4681 aatttttatc tctttcgaaa caagctgctt tttcaagcga ttcttcttta ctgaatcaga
     4741 tctggtcagt atcagaagaa accttcaagt tatgtagtga tttgtttttt atcgacggta
     4801 tcaaacgaga tcgctggatt tggtctggag atgcgtatca atcattcatg atcaatcaat
     4861 atcttttttt tgacgaggaa atcaataaac gaacgatatt agcattaaga ggacaaaatg
     4921 aaatcaagca gcatatgaat actatcgttg actattcgat cttgtggttg attggtatcg
     4981 aaaaccacta tatgatgacg aaagactatg aatttttacg catcgtctac cctaagatgc
     5041 aatcattaat gagatacttg atggagcaaa cgaatgaatt agggtttatt tatgggagag
     5101 caaaagattg gatatttatt gattggtctg aaatggataa agaaggaacg cttgctgcag
     5161 aacaagtctt attactgaaa gcctatcatt ccatgatcac ttgcggagat atcttagctg
     5221 aaacatatgg agagaacgaa aattatcata ctcatctcta tcaagaaaaa tatcaactat
     5281 tgaaacaaaa cctatttgac tacttttggg atgaagaact tggcgctttt atcgattgct
     5341 atgaatctgg gagaagaaat gtgacgcgtc atgcgaatat tttcgctgtt ttattcgatt
     5401 ttgctaacaa agaccaacag caatcgatct taaagaatgt cttattgaat gacactatca
     5461 cacagattac tactccatat ttcaaattct atgaacaaga tgctttatgc aaactcggac
     5521 agacgaatat cgtctaccaa acgattcttg attattgggg agggatgtta tctcatgatg
     5581 cggtaacttt ctgggaagaa tacgacccta ctcaaacagg aaatgaaaag tacagtatgt
     5641 atggagatcc ttatggaaaa agcttatgcc atgcatgggg agcaagccca atctatcttt
     5701 taggacgcta ttttatcggt ttgtatccaa caaagccagg ttatgaagaa tttatgatac
     5761 aacctaatct agattcattt gaaacactga attgtacgct acctatcaaa gatggaaaga
     5821 tttcgcttga aaaaacaaaa gataagatcg tgattacttc tgatagaaca ggcggaacag
     5881 tgaaaatgaa tggtaaaagt tatcctcttc tagcaaaaca gccacttgtc ttaacaagct
     5941 attcaaaaat aaatgcataa aacaaaaaac tgcttgcgac atatcagaag actttaaatc
     6001 ttcttctgat attgtccaag caatttttat tttgaagttt gcttaattct gttctttaga
     6061 aacaatgttt gttctttttc cggcaaaaag aaagctagat aatacgacaa gtaaaaagaa
     6121 agcgatgatt ccaactgtca aggctatttg accgtattct cctagtttcc caagtacaag
     6181 tcctggtact agataatcag tatcggcaaa tgtagaagaa gcgattttaa gatctcctaa
     6241 tactggaagg ataaataatg gcagccaact aatcaataag ctattgaaag cagaacctaa
     6301 taccgcccct cttctaccgc cagtcgagtt gccgaagaca ccagaagcag ctccgcagaa
     6361 gaaatgtcct actaccccag gaatgataac tgtagtttgc aagcctatca tagcaaacat
     6421 ggatagtgtt cctacaaaga agctaacaaa aaagccaatt aatacagcat taggtgcata
     6481 tggaaaaacg ataggaacat ctaatgctgg tttagaatcg ggtacgagtt ttttagcaat
     6541 cccttggaat gctggaacga tttctgccaa caccattcga accccttgaa ggacgataac
     6601 aaacccagct gtgaatgttc cagcttggat cagtgcaaag attacagcgt tgtctccatt
     6661 gcttagttct ctttcaacaa atccaggacc agctaacaaa gcaagtgcga catatacgat
     6721 cgacatcaag agcatgatcg atacagtaga atctcgcaaa aagctgaaac ttttaggaat
     6781 ctttatgtcc tctgtagaac ggtctttgtt tccaaaaaac tttccgatcc atccactaga
     6841 agcataaccg atatttccgg tatgccctaa cgcaacttga ttgtttccag tgatgttctt
     6901 cataaacggt tgacaaagag caggcgtaat agttaatagt gttccttcga agaccccgcc
     6961 taataagatc atccacaaat ttgcagtaaa ccccgcgctt actaaaatga ctgctgttag
     7021 acaagaaaca taaagcattg cttgacctgt aagaaaaatg aatttatatc ttgtgaatct
     7081 agcaagtaaa atattcacga tcatccctac tagcatgatc aaagcagtag gtgtaccgta
     7141 ttgttgcaaa gcaatcgcca ctactgcttc attgctagga actactcctt gagtatgcag
     7201 agctgtttgg aacatcgaag caaaaggggt tagtgaatta ctgattaaac cagcaccgcc
     7261 gatcagtact aggaatccga caaatgtttt gatagttcct tggatcactt ctgaaaattt
     7321 cttcttctga agaacgagac caagcagtgc aatcaaagaa acgaggatgg agggctgact
     7381 gacgattgac acaaacaaat tcaacaattt atccattgat agttcctccc ttgaatcatt
     7441 tttattcttt tgacaagatc ttcgttactt tttcttttac ttcttcgctg tcaatgatcg
     7501 attgcaaagg tacgagcttt tcatcaggta tagaagttag cgtatctttc aaagaatgct
     7561 caatgaagaa ataatcagcc atatcgctag atacacttcc aacatccgta tgacttactt
     7621 caaactcgtc cacatctgct ccaagatctg caagtgcttg tttgatgttc atctctacca
     7681 taaaacttgt tcctaatcca ctttgacata ctgcaaaaat tttcatatca ttcgtctcct
     7741 ttttttatga tcgctaatat ttcttctttt gttgatgcgt gtaataaatc ttgaagattt
     7801 tgtttattag aaagtagctt tgttaaattt gccaaagccc gaagatgtgc ctcattatcg
     7861 atagcagcta aacagatgaa caaactaatg ggatgggctg gattatcaag cagatccaca
     7921 ggttgttgaa gtttcagcaa agacatccct acttcgttta cgccatcttc tggacgagca
     7981 tgaggtaatg cgatgtgatc gccaatatgg ataaacgcac catactgctc cacgcgctcg
     8041 atcatagctt tgatataact ttcttctatt ttattttctg ataatagcgg ctgtgcggca
     8101 aatgtgattg cttcttgcca gttttttggt tgatccgata attggatcat ttcttctgtt
     8161 aataattcag atagcatagg acgttgatcc tccttctttt ctaaaagttt gttcatttta
     8221 cgatttaata cttgatagag cttttcttcg tctacgcctt cttttaattc gatataaggc
     8281 agtagcgccg acaacaattc ttgggcagaa ggcattgaaa atcctggtat cagccaatct
     8341 ctttgtactt gattgatcag ctgatttttt tccaagtcag tcatgattgg atgcagaaca
     8401 tagagagact ttttcttatc agtaagtgtg agaggtacag tagaaaaaat aatatcgtaa
     8461 gaagtttcgt caacctcgtg taagcggtca gctgaattag tctctgtaaa aaagatcatc
     8521 gggaataatt gctgaagttc actttttaag atcaatgaag agctaatgcc acttggacag
     8581 acgatcacgg ctcttatttt tctatttctt tcttctgcgt ccgcttttct gatctctccg
     8641 ccaaatagta tagtgaaaaa accaatctct tcttcaggga tgctcttccc tattcgtctt
     8701 tctaacggaa gtaatgcttt tcttgtcatt tcgtagatgg aagcatattg atgcttaatt
     8761 tgatcgatca tcacattagg taaataaaaa gaataggaaa tacgaaaata ggcagggacc
     8821 aagtgataaa agacattcat caataattca cgaaacgaag caaattctac ggccatcagg
     8881 aattccatgc gatggatgac ttctgaagaa cattccagca aaaaatcaag tgcaggctcc
     8941 tggacatctc cttcaacgat cgatgctaag caaagagaga aaaatataat ctccgaattt
     9001 tgatgttcgg aatagccaag gccagtcaat acttgtcgac taatgtcagt gactgcaaga
     9061 gaagccaaca gttcagcatc ttttaccagt tccatggatg tttttccaat tcgaatggtt
     9121 gtcaaactca aataataagc taattcatcc attcgatttg gtgcgaatcg aagatctgat
     9181 agtgaaatcg ttttatctag caaatctcta actttggcgt aataatcaat ggaaagggtt
     9241 gcggttaaat acgtgagccc aaatcttcca gcaagtgact gcatcaaacg agcagtgcga
     9301 ttttttgctt ctcttcttaa tattgcttct gaccctagca gcctaaaacc agttttccta
     9361 gtatacgaca gctgacaatc ttccacttct aattcatttc ttatcttttt gatatctgac
     9421 aggactgtcc ctttagaaac ctgaagaaaa tcttgaaaat gaaaaacaga aacagacgtt
     9481 gcagtcgaat aaaccatcaa ataaagaaga tctcttcgct cttgttcggt atacacagct
     9541 tcaaatcgag taatatccaa aaatttttct gcccagtcga ttgctcggaa tttctttata
     9601 cggatacggg tatcatccac atattcggca aaaggagtta attgtttgat cgcctcgctt
     9661 gtatttactt cgatgatctc tgctgctttt tctagactga tttctcttgg tgttttcagg
     9721 agataactaa ttttgtagtt cacagcgctg ttcacctcct acttatattc tagtatggta
     9781 gcgcttttaa atcgaggggt gtttggtaca acttacagtt ggattttttt tacgactgta
     9841 ccgaacgatt tctatcgaat ggatacagaa aaaaagcagg tgaccaaatg tctcctgctt
     9901 ttatgaattg atttcgtccg atcaatgatg atcatgttta ttttttgagg tcagttcttc
     9961 aaatttttct ttgacatctt caaccgcttt ttcgatatct ttttcaaagt gattgggctc
    10021 attaggcttt gtttcctctt ctacagaatt tgaaccagat aaagctgttt ctttattttc
    10081 tgtttcacca gagtttgtgc cagctaatga ttcttctttg tcttctgttt caccagaatt
    10141 agttcctgct aaagccactt cttcttctgt tttaggggcc attgaacctc ttaatcgttt
    10201 gtgtttttca ccgtcataat ttgtcatgtt ggttcctcct agtagttcta tactttttca
    10261 tcgtacaata agacagttta tgcgtcaaaa aacaaacccg attcttacaa atatgtcata
    10321 tttttccttc tgattcttga atgcttttga ttccttcttc caaaattttc atttgtaaga
    10381 ttgtttcttc atctttgatg actttttctg cgccattaac taatgtctcg taaatccctt
    10441 cgtagacacg gctgtaatca ccaacctcgg aaattacttt ttcttcatga taactccctt
    10501 gttcatctat ataggtcaat acaccataat gttcgggcaa atcgatacca aagtcttcat
    10561 gatcaggcat gtaaaacatt ttcagatgtt cttcttgtcg atcttttgtt tgtttcacga
    10621 acatcccttt ttttccgtaa acgacgaaac ttggacgttc tttgatgcgg aaataactgg
    10681 attttatcga aattttcata tttccataga aaaaatccag atcgaagtaa tcattcattc
    10741 gtcctttccc taaaagttga cgtacatcat agctgatatc atctggttcg ccgaaataag
    10801 aaagaacttg atctactgta tgacaagcat gtccatataa atagctgttc gcagtagaaa
    10861 attcatgcgt attttctggt acttccggtc tgaaataatc ataatgcatt tctacttcta
    10921 aaatatcccc taattttccg ctttcgatta ctttttggac tgtcaagaag tcagaatcga
    10981 accgtctgtt ttggtaacat tggataaata gacctttttc ttttgctaat gcaaataact
    11041 cttctgcttc ctttgacgtt tctgcaaaag gtttctctac tagtacattc ttaccatgtt
    11101 cgagtgccat ttttccatat tcataatgaa attgactagg tgtagtgatg acaactaatt
    11161 ggatctcagg atcatcccaa agatcagaca gctgatcagt atattcgatg tcttcgaatg
    11221 ttttccacac tggttcagat ggggaaaaaa tttttttgat attgattttg ttcgtaagtt
    11281 ttcttgaaaa aggcaaatga tagcgatttg tactctttcc attgccaaga taagcgattg
    11341 taagcatgag tggtgcctcc ttttattgat gttttcagta tatcctagaa aagcgcagaa
    11401 agtatttata atagctattc tggtaaaaaa gttcagaaaa acacttgtta tcattcggtt
    11461 tggtgaaatt aatgttcaaa gtaaggataa aaatcaaaaa gatagtagcg gaaccaagtg
    11521 tttatcagta agatagaatt tggaggtcag atcatgctta ttgaagaaaa actaacaaaa
    11581 caagagttgt ttaccacgac agagaaacgg attgtcgatt acatccgtag gaatatcgaa
    11641 gctgcagtct acatgacgat tgaagaatta gcaaaagcta cgtatacttc ccattcagct
    11701 atcattcgtt tatgcaaaaa aatgggattc agcggattta aagaattccg tttcgagctg
    11761 gggcgggaag ttcatcaatt gattgctcag ttcaatcaaa cgattgatcc taattttcct
    11821 ttttcaagtg aagatagcac tcagcaaatt gcaaagaaaa tggcggattt atcgatccag
    11881 tcaataaaaa aagcacaatt gcagattgaa catgaagatt tagatcaaat cggtaaagta
    11941 ttgaacaagg cacagcgaat ctttcttttt gcaaaaggtg attctcaaat cacggcaagg
    12001 aaattccaga ataaaatggt caagttgaat aagtttttga tcatggctga agaatacagt
    12061 gattcttctt ggaatgcagc caatttgact agcgaggatt gcgccatttt tattagctac
    12121 agcggtcgca tccatcatta tgaacgaata atgacctatt tgaagcatgt agaggcccct
    12181 accttgctga ttaccggaaa tcagcattca gaaatggcca aacaggctga tatgtgtctt
    12241 gttatatcgc aggaggaata tgattttgct aaagtagcga ctttctcctc tcaaattgcc
    12301 ttcgattacg tattaaatac gttattctca gtgatatatt ctcaaaattt tgaagagaat
    12361 atccgcaact tgaaaaacaa acaagaattg atgcaaaaag gccttcttaa agaatatcgg
    12421 aaaacttaag aaaagatcct catttagtaa taagaaagat agtgtttgat aggctctctt
    12481 tgatatcatc ataaaaacag gctgaagttt tctttttcaa acctcagcct gtttgcttac
    12541 ggaacgattc gaatagatat attatagaaa tgaggaatag ctaacttctg tttcttattt
    12601 tgaacgattg acgaaaaaga tatataagaa ggaggaaaat cagtatacat gcagctaaca
    12661 atccatacag gaaaaatcgt ttgttctcgg atactttctg tacttctttt tgaacttctt
    12721 ttgcatgata aggaacccgg tgccctctga caagtaagcg atgactgttg accatataag
    12781 gtgtacaagt taggagtgtc acgaaatcct gccctttttc gattctcaga tcttttgtat
    12841 cagtcggttc taccgtcttg atctgatcga cttgataagc gagtgtccgt ccattgattt
    12901 cgatatagaa agaatcacct aaagtcagtt tcggtaagtc tgtgaacaac tttgcttgag
    12961 gcagtcctcg atggctggaa ataacggcat gtgtattttc tccccccgta ggataagacg
    13021 ttccttccaa taatgaggca cctttttcta gcagcacagt attcgtttca tcaaaaattg
    13081 gcaaatggac attgatctca gggatcgtca aaataccgat cgtatgtttc tcaaaatagg
    13141 ttctatcctt tttcgcaggt tcttttttct tagtaaaggg atctgcacct ggatttccac
    13201 cttcttttgc taattgctgg tttttctttg tcatgttttc ttggatttta gccatgactt
    13261 cttcattttc tttgaccgct tgttgctgat aatgagagat gatctgctga tccagataat
    13321 tattcagcgc gtcgctaaca aatggataag ctagtatccc tattcctgag agaagcaaga
    13381 aaagcatcag cagatctaga agtttccttt tctttggttt cattcgtgtt ctccttgtga
    13441 tgactgtttg ataataaaag aaaaaggagc atttgcgtaa tccgctgctc ctttttccaa
    13501 ttcttgcaaa aagactaaat ctgactgtgc ttgcgtctag caaagtacag tccagcaatc
    13561 agtagaagga ctactcctgc accgatatac acatagattc ccttaccgcc tgttgaagga
    13621 agtgtacctt tgtgtttatt tggtattttt tcaggagaaa tcaactgtcc tgctgttaca
    13681 taagattgtt catcgatagt aaatgctaca cggtttgtta atggaacata tttttctggc
    13741 gctttcgttt cttccagata gtacgtgcca tattttagac ctgtcacatc gattaaaccg
    13801 ttacttgtgg ttgtaaaaac cgttgctgat tctttcgccg atacccagct gacggctttt
    13861 gtggatgggt cgatcgataa atatttcgct gtgtcactat cttgatcacg aacgacgaat
    13921 tctgctccag caagtgtttg atctgatgta acgtcaccat ctactttaac aaatcgtttg
    13981 cccccagtaa cgacatcgac tgacggtggt gtttgatcat ttgtatgccc gttatcgaca
    14041 tttgcttggt tagaaaatcc tttggttgga tctgcttttt cgttcaaatg catatagtaa
    14101 acaaatttca gcgtaccacc aggagtgagt gaaggaatat aattcgagtc aactgaaacc
    14161 gtgaatccgt ttgtttgttc agtgacagaa taatttgttg gatcgatttc tttatcccca
    14221 tcatacaaga catacgcata cgttcctgaa gtttcatgat caaatgtcaa agcggcgtca
    14281 tgagtatcaa tgagtttgaa tgtcgtgtac ttgttttgtg ttccttcttt atcagcgata
    14341 cctaaaggaa tattgacaga gatctcatat tggatttttt ctccgattgc gacatctttt
    14401 ccattcaatt gaggtgttgt tttatcaact ttggacgtat catttttgat tgtcttttct
    14461 acaggagttt gactatttgc caagatctca aacggcgtgt ttgtttgatt atcaatcatt
    14521 tccgcattat caggagcttt tacttcttct aaattatatt ttccaacctc taaatgattg
    14581 acgatcaact gacctttttc aatcgttgcc tcggcaaagt cattgttgcc gatgtcataa
    14641 gaatgaccag taatgaaatg tttggctttg gtttgatccg ttgtccaagt gtacaatcca
    14701 tctgtgacac tttggatgta ttttttgacg cttggtcttc cttcttcttt agaaataata
    14761 aattctgctc catttagtgc ttcgttttcg gcagtaccga tttttgtaac tttcaacgtt
    14821 ccatcattac cgactgtatt tttagggtag agatggatag tatctagttc ttctgtcccg
    14881 tatttataag agccatctgc ttgtttgatc atctcatata cagggaaagc taaaaccatg
    14941 tttgcggcag ctgacactcc gtcttttggt tcttctttga tcgtgtagac tgcatctttc
    15001 ccattttgtt ttttaggtaa agataaagtg acatttccat cagcatctgt cgttcctgaa
    15061 gcaactggtg taccaggagt caaagactgg actgcttgtt ttgctgcatc cacggacgct
    15121 cctttatctc gttgcgcata aaattcttga gtgacgttat aaactgaaaa tgaaatatcg
    15181 gctaatcctt ggtattgatc gaattcgctc atttctttcc cgctgttttg gattaaagga
    15241 tcgggtaaat cagtcatttt ctttttatga aggatgactt gagctgctgt ctcctctgca
    15301 aacacttttt ttgcgccgaa gctgtttgtg agtaatggta aaataaggaa aaggactcct
    15361 aacataacgt ttattttttt atgatttttc attttttcac tcctgttctt tttttgataa
    15421 acaaccagat tgcaaagctt gcgaatacca taccaacaac gcctaaaagt gtatttttcg
    15481 tttcgtttgt ttttggaaga aatggacggc tattttcttt tttattgact actttcaata
    15541 tactgtccgc ctctttatcc tcagaaagaa aagttgaaat ggtaaacgga atttctgttt
    15601 cacttaaaat ataaccagaa ggtgcttcga tctcttttaa tcgataactt cctgttttca
    15661 acccggaaat tgaaaaagcg ccatttttat cggaaatcag gtgaagcgca tctgattcgt
    15721 tctttgtcca cttgtatccg ttggctgttt gctggaggta atttcctgct ttgtttttaa
    15781 taaggaatac tgcgccagct aatcgtttat ctggtttatt tgtctcgatt ttgacaaatt
    15841 gcttactccc tgtatataca gagactttct ttgtactgac accaaaacca aaatccagtg
    15901 ttccgttgtt gttgatttcc ttgttcgcct gtgctgtact gcttagctgc atctgataag
    15961 aaatggtcag tttttttcca gcatattttt ccaacgaagg gatcgaaaat aaaacatcaa
    16021 atccgtgagg tttcttgtgt atgacaaagc cttctgtcag cttttctcca ttcgatgaaa
    16081 tcgttaaact attaggtaaa aacgttaatg cttcatccgc actatctgac aattcgaact
    16141 tttgatagtc aaggatattt acaggaattg tcgtatgcaa ggaataagtg attttttcac
    16201 caatcgtaaa atcatttcta ggctcttcta ataccttctc aaaaggtgga tcagggtagt
    16261 cgttttcttc atttttagga tacaaatgga tagttgaaag tttttgattg ttttgtccat
    16321 gtacaggaag aacaactacc atattctctg ccttttcttt gacgacttct ggtactttgg
    16381 attcaatgaa aagatacgct ttatctcttt tttcttgatc tttagcggcc agagaaaaag
    16441 aagcgatacc atcttcgttg ttaagagttg tagttgttgc ttctgcggta aacataccgg
    16501 aagacgcacc gttttttgcg atctctgctt gtgcttcttc taccgttttg cccttttccc
    16561 gtagatggta aaaagaatct gtgacatcat aaacttggaa tgtgacacca tttaatcctc
    16621 gatacgtttg taataaagct ttttcttgtc cgtcatttgg atgatttttc ggcagttgcc
    16681 cgtttgggaa aagcaatttg tgtaaagtaa tctgaaccat ttctgtttct gtttctgttg
    16741 ctacctgagt aaaagctggt ttaaatagta acaagaagag acacatacta agccaaccaa
    16801 gttttttcat atttagctgc cccctctttc ttttttcaaa aaaagatacc aaatagaaaa
    16861 cgcacaacca atcatcccta ctagatagat tcctaaacgg ccaattcctc ccgtttcagg
    16921 taatggtact tttgcctgat tcgtgatgtc taaagaaatc tggttgtttt tggctcctgg
    16981 tgacagaaca gattcatgat cttgtccatc cacttgaatt gacccatctt cgtgtataac
    17041 tatagtaact ggctctttta gaccttggta tccttctggt gtaaaagttt cggtcagcgt
    17101 ataagttcca ggttttaaat tttcgaatag aaaggtatct gtttcttgtc catcttttgg
    17161 cgattctaag tccatttctg gtccctgcag tctgaatttt gctcctttta atgtctgtcc
    17221 gttgtcttct tttttgtgta ccgttagatc aaacgctttt agtcgatttt gatgagtgag
    17281 cgtccataca ttatttgtca tctcggtatt ttttcccgtg taaatgattt ttccatattt
    17341 atcgatttgg aattcataat ttcccggcga agtatcatat ccggcaggac cagctgtttc
    17401 aacgactcga tatttaccag gtgtgagtga atcaaaactg gcaagacctt tttcattcgt
    17461 tgtttggctg tcaattggct gataagtacc atttgcttct tttttctgta gggaaaatgc
    17521 tgctccttta agatttatct ctttgtccgt cccttgcatc gcatacttac gtactgctac
    17581 aggaacttca acaaaaggat tttcaatagt caactggatc gtatcatcgg aagtagtgac
    17641 tgtttttcca tcaatggtta ccgtaccatc agaagcgatc ttgatttccc aagtagtctt
    17701 tttgcttaat ccatgccctt ctggagcttt tgtttctgtc agcgtatagg tcatttcttt
    17761 ttgcaatttg acattttctg gaagagaata ggttccgtcg ccatgatctg ttagtaaagt
    17821 atcaatagaa tctcctgtta atttgaaaac agcgccaata agatcctttt cacctgtaga
    17881 ggaatttttc gttattttca agtttaacgg tgtgaattgt ttagtatttt tccatgtcat
    17941 tgcatctatt tgttgagaat cgtatcctgg tacaggtaat tctttgattg tttggtaact
    18001 gaatgcttga ccttgattat tgtattgagg tagtgataat atctcttgat aactttctcc
    18061 gctatttgca gataatttgt caatgtctgc acgttcccac gtatttgttg tatctttagc
    18121 tggtttaatg attcgaatat atccgttttt ccaagctgca gcatttgtcg tatgttcccg
    18181 ttgaatctca aaagtaactt gatctggacg atcagctaga ttgttgtcaa attcttccca
    18241 taactttttg atgtgaagac tgactccggg agctttagca gatggtatac cgaattcagc
    18301 taattcattg gtatcaatgt ttggctggaa agttgtcctg ccgttcattt gataccagaa
    18361 atttggatgg aagtcctcat tttctgtttg gattctcact tggtaatgga tttggatttc
    18421 ttggtctttt cctaaataga tctgattgct cttgatggta tttccttcta tggaaataga
    18481 tggagtgacc gttgtaggac ttgtccccac actcttgact gaaagtgtac caggctgata
    18541 aatgaatggt tctgcgattg gatcatttat ttgtccattg cttacagttg ctgagatctg
    18601 tacagctttt ttggctaggt attcagagat atctgttgca tgatcagctg attcatagta
    18661 aagatcgcct ttttcatctg atgaaaccat ttgtcgcata cgagactcta cttctgcttt
    18721 tgagagacca gctgccggat cgctttgaag ttggatgcca agaccatgta tttcgattcc
    18781 tcgttctttg agtgccatcg cttctccgat ggttgcgata aacgtactgt cgattcttct
    18841 ggatagattg ttttggtcag gtgcatcata gattcttgag attagagaag tatttcccgg
    18901 ccgatcttgc gtattagaaa actgagttcc gtaataattg ctgcttgatt gtgcgtgtac
    18961 ccgctgtact ttataggaaa atgttggtac accatccgtc agcaaaacga tcactttttt
    19021 atgtccattt ggaacggata gcatgcttcc tgcatctctt agtgcttttt gtgtaaaagt
    19081 accaccattt gtccgtgaag gtgtaatgga ttttacttga tttttcactg aatcaaatga
    19141 acccatctgt actgcaccgt tactgtagct atatccttcg cttgagtagc cgacatatcc
    19201 catattgatt ttgtctgtga taccgctatc tgctaaagta tcgacaaaac gatcgacacc
    19261 aatctttact tcaccgatcc gattattgtc gttcatactt cctgaccagt ctacgaccaa
    19321 tacgagatca agaggcgtga tatctttttg tacatttcca cgagcattca aataaacatc
    19381 aaacaatccg ggtgtacttg tttcctttgc atactttcgt atcgcatagt ctgcttgatt
    19441 accggttcct ccatattcga tatacgaatg ggtccgatca ttaggatctc cattccaaga
    19501 atttatacca tcccattgat ttgttccgtt tttattgcct tgatgatcaa gaacatttgt
    19561 atttcctgta ggttgccaac tggctttagg ataagtgccc tgatcatctg ttgtatattc
    19621 tgtaggaatc gtttgattag atggcacact cacagctggc aaggataatg taggcaatgt
    19681 aaatgggctg acagaatttt cgttttcttt tgatgattct tcttctattt cgtctggctt
    19741 tggtactgac acatctgtat ctttcttatt ttctgtctca tcaataatgt catattcttt
    19801 attatcgacg atcagcttgt ttgcctggct gtggtaaagt gctggatcga ttggaaaact
    19861 cagatcgata gtttgtttag tgtttgatga gatttccaac gtaattttcc cgctatttac
    19921 ctgataagca tagccatctc cttcttttct ttccacatca cttaagtaat tttcgactag
    19981 accaatttct ttttctatct tttcagcact ggtgttattc gcatttagct tgatgttcag
    20041 tgcttttttt ccagactgat ccgaaatgac ttcagctgaa attgtttcta aaactgttgg
    20101 atcatctgta gtgtctgcta ttgcagataa tggcacaaag tttgataaac tcaatattat
    20161 cagcatgaaa ataactttca gtttcttccc tgtggtcgtc atttttcccc tcctttttct
    20221 ttttttctgt tttgacgtga atagtgaaaa ttattaaaat aaacaggcgt tacataattt
    20281 tatttatttt aatatgataa ttattatacg aagggcgcta aagggaatca caccaatttt
    20341 cataccaatt ggtttcattt atgaagttga ttccttcgat taatgagcgt ttcattgaaa
    20401 gaaaagggtt gagggagaaa aacatatgat caggttgttg gaatgaaact atagaaagat
    20461 ggaaaaaatc attcgaattc tgtccatttc tgacaagtgg atgactaaaa aagaacttgc
    20521 caaacgaatt ggtagttcag aatctacatt tgtaaggtat atcgaagaga tcaaaatccg
    20581 ctggggaacg gatgtaacga ttcaatcttc acaaaaattg gggtatcgtc ttgaacattt
    20641 gaatgtacca gcttatttaa atgttctgat ggaaatgtcg caaagttcta caaacagtcg
    20701 tttattaaat gagattattg tcaatccggg taaaacgatt gagtattatt gtgaaaaaat
    20761 ttccatcagc cggtcttctt ttacacgtaa gttgaaacaa tgcaatcaaa ttttagaaag
    20821 ctatttatta aaagtgatcg ttgaccaagg ttttcagtta actagcctta aaagtgagct
    20881 ccagttgcgt atctttgtta cctttttctt tttaagttac tacggaagac gcacgttgcc
    20941 atttccaatc gaaaaaaaga agatcaaaga aattatggtc aagaataatt gtcagatgaa
    21001 tttgtttcag gaagaaagcc catatgagca atctttcttt attatgtatt ttgttgtgca
    21061 tctcatgaga gaatcccaag gaactccatt tgaatggaaa gctggagact cttatgtaca
    21121 gttgataaat atcgataaaa aagacttctt aatcttgaaa aatctcttag gaaaattgaa
    21181 gtacagcagc tatataaaag tagtgaacta ttttgtcaat acgatttttt cagaattttc
    21241 ggagaaagaa aaaaatgaaa tcagtgagca gatacgaaaa aatatgtcct actgttcgtt
    21301 ttcatccgct gattgcaaag aaaaaaacga agaatatgtt gttcaattat taacgcaaat
    21361 ttattttttc tcccgtattt tcccatacga tacgactcat ttgacgatcc gtgcaactta
    21421 ctttgcacag cagatgcaac accgccagat acgtctatat gctggatttc gtgcagaact
    21481 tcaattttat tccactctac tgaatcagaa tctgatttat tatttatcta gctatatctt
    21541 ttggatgctt atcggtaatc ctgaaattca ccattatact tctgataaaa aaatactgat
    21601 tatcagcgac ttaagtcttc gacatagtca atatattgaa gaatatatca gtgatattct
    21661 tgctgtgcat aaaattcact ctgagacaac tgctattact gaagatcagc tgtcgaaata
    21721 caatcttctc gagtatgatc tgatcgttac gaatcaaccc attttgaatg cccatgttcc
    21781 tcatatattg attgatgata gtgtgtcatt cgcaaatgaa gaagaactca tccgcttatt
    21841 cgagttatga ataatcaaaa aacagctatg tataaaccac cctgataaaa atttcaggac
    21901 aaatacacat agctgtttct ttttattgta ttccttcagc aatccaacga gatttatagc
    21961 gaaagtcttt taacaactct tcttttgtcc aattatttgc aatgagatcc gtcaaagtgt
    22021 tcattacgag ggtcatggaa aagtatagga aactaactgc ttcttccgac gattgaatgg
    22081 agaaatgatt gattcttaat aattccagcc atttttcgtt cattggagaa tcagccgata
    22141 gttctctacg tttggttgtc tgctgatcat ttcgctgggt taacaagtat aacctttgcc
    22201 aatagatact ttcgtgatct aagtcgcagc accactctaa atatttttcg atccccaaaa
    22261 agaaatgttg tttgttttcg ctaatgaatt ttaagatatc ttgatggact atgtgagaac
    22321 tgcttttgat cgtatacaaa taagcatctt ccaaatcttg aaaatattta taaaaagcac
    22381 cgcgcgacat ccccatcttg tcaacgattt ctgagacttt gacttggctg atgtgctgtg
    22441 agcaaaatat ctccagtaat aggtcatcga ttctctgttt ctttgcgttc gataaattga
    22501 aaaaagtttc ttttggcatc cacatctctc ctttatctct gattatacgt ggatagccct
    22561 tcattgtcat gaaggattca ttccttcttt ttctcttctt ctgtcaagta cccatcttcc
    22621 attcgataaa cgcgatcact ccattgaatc atacggacat catgagtaac cataatagtc
    22681 gctttttgtt tctcatgagc ttctttaact agtaatttga cgacttcata tgcatgatct
    22741 gtatccaaac tagctgttgg ttcgtctgct aagatcaagc ttggctcatt atataaagcg
    22801 cgagcaattg ccactctctg tctttctcca ccagaaaggt ctttaggata actattttta
    22861 agtgactgga tatctaaaga ttgcagcaag gaatcgattt ttgttgaatc ttcttttttt
    22921 gaagtaactt ggtcaactag ttcaaattgt tcctttaccg ttaaaaaagg aatcaaatta
    22981 gagctttgga gaataaatcc tatttcttta aagcgtaaat ctgctcgttt tttttctgga
    23041 agctcagtta gtgagtgacc gttgatcact atttctccgg aagtcggtgt ttgcaatcct
    23101 cctgaaatgg taaggaaagt acttttacct gaaccagaag gaccaatgat gctgacgaat
    23161 tctccttcct ttacataaaa atcaattgct ttcaaagctg agacctttgt atgaccagaa
    23221 ccaaatgttt tggtaacttt tttcatcgtt aatatttttt tcatttttat taacctccta
    23281 ttgctgtaat tgggtctact ttggttatcg tacgtatcga aaacaatccg cctagcattg
    23341 cgacaatgat caaaatccca ctatatagca accattgtga ccaaaaaaca gcaaaaggca
    23401 tcgcgcttgg taaaatcaga ctagtcaaat aagcaaacaa aagagcaagc agaacgccta
    23461 ccaccccaac aataaaggac tgtgctacta aagatttagc gatgaagaaa tttttgatcc
    23521 cttgtgcttt cattactcca aaaatagcag tcttctgaag tgtcatcacg tacatgaaaa
    23581 taccgaccac ggcagcaacc actaagaaca aaaagtatat catcgcattt aaggtcatat
    23641 tttgggcaga atagccagga atactttcaa tgaatgtccc aatagttaat aactgtagac
    23701 ccttcgatgc tttttctttc gaaattttag ctgaatcctt tattacgatc ccattgatcg
    23761 gctgttcttc ttcagaagca aacggctgtt gtccatactt caaagcagtc cacgtatcta
    23821 gggacgcata gatgacggga gaaactgtgt aataagtttc aggaaagata ccgacaatcg
    23881 tcaaaggatc gtcgtaactg ccaattttga tcgtatctcc aattttatag cctgaatcag
    23941 ccagattttg agagatgatg atttcattct ttgttttaaa ttcatgccct ttcgtgagtt
    24001 ttggtaataa aaatgcttct ttcgttgtgc cgaaaactgt aacattttct ttgttttttc
    24061 ctttgattgc tccgctgtac aatccgattg gtgctttctc tcctccttca atgtatttca
    24121 gatctcctct tgttaattgt gaagctgcaa atacttgatt tgcatcctcc gacaaaacaa
    24181 tctcttgtgc gtcccaatca tcgattgctt ttttaaactc ttctgaaagt ccattcgcta
    24241 gtcctgacaa catgaatacg acatacgcaa tcaagaacat aataccgacg atcaagccat
    24301 atcgtaattt agaatagcga atttctttga tagctagaaa catttgtttt tcctccctat
    24361 gatagattga tagtgacaaa atgtcacttg ttgaatagtg taactctaaa gtgacaattt
    24421 gtcacttgtg ttgttttttt cttatcaata aattgtctaa gacatctttc gataaaacct
    24481 taacatgagt cataaaaaat ttatttaagc cttatttatg ctttccatga taaactaaag
    24541 tgaccaaatc aatgaggtgg gaaaatgatt aaactattag tagtagaaga cgataaagta
    24601 ttatccaata atataaaaga aatactcgct gcaatcggcg agataacaca agtttatgat
    24661 ggtagtgaag gactgttcga gttggaaagt ggagtttatg atcttgcaat cttggatttg
    24721 atgatgccgg aaatgaatgg atatgaagtg ttagcaaaaa tacggaaaga aaaaatacag
    24781 actccagtat tgattttgac tgcaaaagat agtttggaag ataagattga aggatttgaa
    24841 cgaggtgcag atgattatct aacaaaacct ttctatcgag aagaactgat catgcgcgtg
    24901 aaagcattat tgaaacgctc gctaggtctc tttgatgaaa atgttctgaa gtataaggat
    24961 atcagttgtg atttaaaaag caatgaagtc ttgtataaag aacaagtttt gcccatacaa
    25021 ggaaaagagt ttgaattgct cgtttatttt ttacaaaata agggaatcat tttgacaaaa
    25081 gagcagatat ttgaccggat ctggggattt gattctgata cgacgattac agttattgaa
    25141 gtttatatga gcaacttacg aaaacatctg cgaagtactg gattggataa agaaatcaaa
    25201 accttacgta atgtcggtta tatactgcaa agggtgtgat gcaatgaatg gagatattac
    25261 acgaaagcaa cgtagtcgct tttttgcgac aaatgtagca gcatttgctt ttatcttttt
    25321 tcttttggga ttcattactt tacagatatt aaaccaatct gcatacagag aaacagatct
    25381 gtcattagag aaaataaatt ttgattctcg aacagtacaa ttagagatac aaagatatca
    25441 gcaaggcgat ccctttttaa acccgactca aacggaacaa aacagaacaa acatggaaca
    25501 gccattaatg gaaccatcaa atgaccgttt taacactcaa gtgatcttgt ggtctaaaaa
    25561 tggtgagata ttgaataaag cagctattgg tgggcgtttc aaccaaatca aagaattaaa
    25621 aatggatacg aaaaatctga ataccatcca aaatattgaa ttaaatgaag acagtggctc
    25681 atctgccctc tctttccgtt cgattacgaa aaaagcggag acgaatcaag atggcgttgc
    25741 ctacatccaa attttagaaa atacaaatca aatcaagaat tcattagcga cttttcgcac
    25801 gattatgatt ttgtgtatga ttgttttttg gattctctct attggtatca gttattactt
    25861 gtctagtcta agcatgcgcc ctattattct ttcttggaga agacaaaaag aatttgttga
    25921 gaatgcttct catgaattgc gcacaccgtt aacgattatt caaaatagtt tggaacatct
    25981 ttttaccaag cctaatcata ctattattga agaatccgaa agcatcgctc aagcattgtc
    26041 agagacgagg cgcctcactg gattgacttc ggatttgctg acaatcgctc ggaatgattc
    26101 tgatcagcag ctgctttcaa agcaaatgat caatacacaa gaatatatcg aacaacttgt
    26161 taaaccattt caagagatgg ccatcataga tggaaaagag tttattctcc ataacgatgc
    26221 aacattagaa gtaccaattg atgaaaaaaa aatccatcaa gttttggtta ttttgctgga
    26281 taatgcatta aaatatacga atccagaaga taagatcatt ttggattcat ctgttaacca
    26341 taaagaatgg ctgattcacg taaagaatac tggcccttct atcagtgaag aagatcaagc
    26401 aaggattttt gatcgatttt atcgggaaga tcaatctcgg gcgaaagaga ctggcggata
    26461 cgggcttggc ttggcaattg caaaacaggt aatcgaacag catcatggac agatttctgt
    26521 atccaattat cttccacaag gagtcgattt taaagtccaa ttgccgttga agcctaaaaa
    26581 ataatgctta tccctaaaaa agggctgtga caaaaatctt gtcacagccc ttttttgttg
    26641 cgaataaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt ctttaagctg
    26701 accacttctg tcacaacctc tttagcttac gcttacgatt ttttattgcg gtcttcaatg
    26761 tgtacatcac cgcttgtgtc aatatccaat tcttctcggc taacatcttc tgtcactttt
    26821 tcaacatctt catgttcttc tttatgaatc ccaacttctt cacgaataac tgtatgtttg
    26881 cttacatcta cttgctcttc tttgattggg atagtaatcg tgtcatcttc caatttttcg
    26941 tctgttccct gtgaagattg atctgttact ggtttgcgtt cgatgacgat ttcttcacgt
    27001 ttgaccggta cttctaccgt ttcagtatcg ttgaccacat gtttgtggat gtcgacttct
    27061 ccagttgtca catcatgtgt atttacatct aatttttctt cttttagttg aattgtatca
    27121 tcatctagat ttggtttgcg atcgatgtct tcagtcttgc ttgtacccat tgtatcagtc
    27181 tcgccaggta cgtccatcgg atcagttcct ggtgtcatat tcaatggatc cgttgtattt
    27241 tgaaagcctg tcgtgccacc tacgtccatt ggatctgttc cagtgccagt ttctaatcca
    27301 gtccccattg tgcttgctgg gctaacagta tcttgtgtat ccattgtatc ttctgttgtt
    27361 tctggctgac gatagccttt tactgtgatg acaaattttc catcagaaat atctcctcta
    27421 tattgatata aaggatcatt ttctggtgta gccgtctcag aatcataggt atcaaaagaa
    27481 aaagtatctt ttattttttc ccataaagaa cggtcttctt caccatccat ccgattttca
    27541 tctgtttcaa catctatacc agacaatccg tcaaatccta gtctagatgc tgcttcatca
    27601 gtagtataaa taacgatatc atctcgttca tacccttcgt tacgcaaccg ttcaattact
    27661 gataatgctt cttccctcgt actatagctt ccgatgaccg ttaaattttt ctcttccatg
    27721 taaattcctc ctagcaaata gatttatgat tggtatat
//
LOCUS       DAZUMM010000031        27582 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000031 DAZUMM010000000
VERSION     DAZUMM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.031"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(174..1754)
                     /locus_tag="ACG8E6_001276"
     CDS             complement(174..1754)
                     /locus_tag="ACG8E6_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFS5061703.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2155..3531)
                     /locus_tag="ACG8E6_001277"
     CDS             complement(2155..3531)
                     /locus_tag="ACG8E6_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS5061704.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3659..4747)
                     /locus_tag="ACG8E6_001278"
     CDS             complement(3659..4747)
                     /locus_tag="ACG8E6_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5061705.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4919..5353
                     /locus_tag="ACG8E6_001279"
     CDS             4919..5353
                     /locus_tag="ACG8E6_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5061706.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5431..5964)
                     /locus_tag="ACG8E6_001280"
     CDS             complement(5431..5964)
                     /locus_tag="ACG8E6_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFS5061707.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6058..7236)
                     /gene="mutY"
                     /locus_tag="ACG8E6_001281"
     CDS             complement(6058..7236)
                     /gene="mutY"
                     /locus_tag="ACG8E6_001281"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFS5061708.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7229..8026)
                     /gene="recX"
                     /locus_tag="ACG8E6_001282"
     CDS             complement(7229..8026)
                     /gene="recX"
                     /locus_tag="ACG8E6_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFS5061709.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8133..9515
                     /gene="rlmD"
                     /locus_tag="ACG8E6_001283"
     CDS             8133..9515
                     /gene="rlmD"
                     /locus_tag="ACG8E6_001283"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS5061710.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9689..10267)
                     /locus_tag="ACG8E6_001284"
     CDS             complement(9689..10267)
                     /locus_tag="ACG8E6_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFS5061711.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10589..11059
                     /locus_tag="ACG8E6_001285"
     CDS             10589..11059
                     /locus_tag="ACG8E6_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFS5061712.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11167..12393)
                     /locus_tag="ACG8E6_001286"
     CDS             complement(11167..12393)
                     /locus_tag="ACG8E6_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5061713.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13147..13347)
                     /locus_tag="ACG8E6_001287"
     CDS             complement(13147..13347)
                     /locus_tag="ACG8E6_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5061714.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13481..14089)
                     /locus_tag="ACG8E6_001288"
     CDS             complement(13481..14089)
                     /locus_tag="ACG8E6_001288"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFS5061715.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14174..14644)
                     /locus_tag="ACG8E6_001289"
     CDS             complement(14174..14644)
                     /locus_tag="ACG8E6_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFS5061716.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(14692..15606)
                     /locus_tag="ACG8E6_001290"
     CDS             complement(14692..15606)
                     /locus_tag="ACG8E6_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5061717.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            15752..16555
                     /locus_tag="ACG8E6_001291"
     CDS             15752..16555
                     /locus_tag="ACG8E6_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFS5061718.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            16733..17680
                     /locus_tag="ACG8E6_001292"
     CDS             16733..17680
                     /locus_tag="ACG8E6_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFS5061719.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(17748..18041)
                     /locus_tag="ACG8E6_001293"
     CDS             complement(17748..18041)
                     /locus_tag="ACG8E6_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFS5061720.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18069..18734)
                     /locus_tag="ACG8E6_001294"
     CDS             complement(18069..18734)
                     /locus_tag="ACG8E6_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFS5061721.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(18874..19506)
                     /locus_tag="ACG8E6_001295"
     CDS             complement(18874..19506)
                     /locus_tag="ACG8E6_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5061722.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19513..20580)
                     /gene="liaS"
                     /locus_tag="ACG8E6_001296"
     CDS             complement(19513..20580)
                     /gene="liaS"
                     /locus_tag="ACG8E6_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFS5061723.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20577..21308)
                     /gene="liaF"
                     /locus_tag="ACG8E6_001297"
     CDS             complement(20577..21308)
                     /gene="liaF"
                     /locus_tag="ACG8E6_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFS5061724.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(21476..21955)
                     /gene="greA"
                     /locus_tag="ACG8E6_001298"
     CDS             complement(21476..21955)
                     /gene="greA"
                     /locus_tag="ACG8E6_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFS5061725.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22091..23266)
                     /gene="mltG"
                     /locus_tag="ACG8E6_001299"
     CDS             complement(22091..23266)
                     /gene="mltG"
                     /locus_tag="ACG8E6_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFS5061726.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            23431..24591
                     /locus_tag="ACG8E6_001300"
     CDS             23431..24591
                     /locus_tag="ACG8E6_001300"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS5061727.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(24631..25578)
                     /locus_tag="ACG8E6_001301"
     CDS             complement(24631..25578)
                     /locus_tag="ACG8E6_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS5061728.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(25711..27102)
                     /locus_tag="ACG8E6_001302"
     CDS             complement(25711..27102)
                     /locus_tag="ACG8E6_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFS5061729.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            27228..>27582
                     /gene="tagD"
                     /locus_tag="ACG8E6_001303"
     CDS             27228..>27582
                     /gene="tagD"
                     /locus_tag="ACG8E6_001303"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047348 - glycerol-3-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HFS5061730.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTP"
BASE COUNT         8453 a         5540 c         4416 g         9173 t
ORIGIN      
        1 taactagcac cacgggtatt tttatatttt tttgattaga catttctatg attttaatat
       61 agtaaaaaga gccgtcacgg aaaatccgtc acggctctaa acaaaatatg agaggctgtt
      121 tttgtttggc tattcttatt acttgatgca gggcagcttg tgtcaagact tctttacatc
      181 aaacttttta attttacatt aggatattta tccgcaaacc agcgttctgc aaattggttc
      241 tcgaataaga acaatggttg gtcgaaacgg tcacgagcca agatattgcg acttgaactc
      301 atcttctcat ccaaatcttc tggatcgatc cagcgagcga ttttgtgtcc cattggagac
      361 atgattactt cggcgttata ctcatttaac atacgatatt ggaatacttc gaattgtagc
      421 tgaccaacag ctccaatgat atattcttcc gttagatatg ttttataaag ctgaatcgct
      481 ccttcttgaa ctaattgata gattcctttg tggaaggatt tttgtttcat gacatttttt
      541 gcagtcactt tcataaacag ctctggagta aatgaaggga gttcttcata agcaactttt
      601 agttttcctt cgaacaatgt atccccaatc tgataattgc ctgtatcata tacaccgatg
      661 atatcgcctg ctactgcttt ttcgacgttt tctctcgaat ctgccataaa ttgtgtgaca
      721 ttgcttaatt tcagtttttt atttgtacgt tccaaccaga catccattcc tcgttcgaat
      781 gttccagaac agattcggac aaaagcgatt cggtcacgat gggctggatt catatttgct
      841 tggatcttga atacgaaacc tgaaaattct tcctcataag gacttaccat ctgatcatct
      901 tgtgttttgt gtccataagg agctggtgcc aattcaacga aagtctctaa aaaagtctgt
      961 acaccgaagt tagtcaatgc agaaccaaaa aatactggtg tctgatcgcc acgagcaatt
     1021 ttttcttgat taaatgcatc tccagcctct ttgattagtt ctacttcttc taagacttgt
     1081 tcatataagg aattttcagt tagaggatga ttttcgggaa gcagtccatc ttcatccaat
     1141 tctactaatc gttcatcctg ataattttcc ggacgataaa actctacacg attatggtag
     1201 atatcataaa gaccttctag ccctttaccc ataccgattg gccaattcat cggataagat
     1261 tcgatatcta atagctcttc taattcttct aacaattcca atggttcacg accatcacga
     1321 tctagtttgt tgataaaagt aaatatcggg atgccacgtt tttttacaac ttggaataat
     1381 ttctttgtct gtgcttcgat cccttttgca ctatcgatta ccatgaccgc actatccacg
     1441 gccatcaaag tacgataagt atcttcagaa aaatcttcat gtcctggtgt atccaagata
     1501 tttactcgtt tcccttgata atcaaactgc ataacagaac tagtaacaga aatcccacgt
     1561 tgtttctcga tttccatcca gtctgatttg gcaaaattac ctgttttttt gccttttacg
     1621 gtcccggctt gacggatagc cccaccaaac aatagtaatt gttctgtaat cgttgttttc
     1681 ccagcatccg gatgggaaat gatcgcaaaa gttctgcgat tgtccacttg ttgctttaat
     1741 tctggattat tcataaccta actccatttc ttccattcaa ttatttatat tttctctagg
     1801 cacagattgt ccgatctctt aaagaaagac aatttcatat agggctgtga caaaagccgt
     1861 gtagcagtct ctttttccga gtaaacggcg ttctagagac agcctatcag caataagccc
     1921 acccaagcca aaaatacgaa aagctgtttt tgcttggctg ctcttattac ttaatgcagg
     1981 atagcttttt cacaaccttt gtcaactact acaactgaat cagtatagca tggattcatc
     2041 gaatgaaaaa gccaccaaac cataagagtc aaatattttt ttgtcagctg actaaaagaa
     2101 aagacgtgtt gcttgtttat tcaagcaaca cgtctttcat aaccaagttt ccaattatct
     2161 tcttggctcg tcatcttcga attcttttct gaagtatctt cgttcttctt caggttccgc
     2221 ttctgtttct tccggatcat gaaaaatgac acggatttta aggattcgag aaccttccat
     2281 ttcttcagaa accagcgtaa tattatcgac atcaaacgat aacttttcgc cttcatccgg
     2341 aatcatgcct aatgctgtaa tcaaataacc agccatcgta tctacatcac tcatatgcag
     2401 atcactgtca aaagcttcat tgaactcatc aatcagcata cgtccttgga tgatatattc
     2461 gtgttcatcg attttttcat aaagattctc aacttcatct gattcatcat cgatttctcc
     2521 gacgatttct tctaaaaggt cttctagtgt tgctaaccca acgacgccgc catattcatc
     2581 taataaaata gccatctgat tctgcgtctt tttcatttca tataacagat catcaataaa
     2641 gaccgtttca ggaacgaata aaggctcctg catgattttc ttgatatcta aattatcaaa
     2701 accgaattta tgggcagctt ttagtaaatt ctttgtatga aggattccga ctactttatc
     2761 tttgtcttcg ttataaacag ggattcttga atagttttcg gataaaactt cattcacatt
     2821 ttcttccaca gtgtcgttga tatcaatcat aaatgcatcc gttcgtggga ccatgacttc
     2881 tcgggcaact ttcgtatcaa gagaaaaaac cccttggagc atttctaatt cttcattatt
     2941 caatacgcct tcgttttcaa gcatataacg catttcatct ctggtcattt ttgaatcttc
     3001 atcatcaaaa gtcatcggcg tgatacgcgc caataaactt gtggaagcag aaagcagcca
     3061 gacgaagggc ttagctacta caccaatcaa gcgtatcagc cctgaagtga actttgcgac
     3121 ttcttctgat ttatttaaag caatacgttt agggtacaat tctccaaaaa caatcgatac
     3181 ataggtcaaa atagccagca caatgatatt tgcaatattt tttgccgctg tgccgccacc
     3241 taataccgga gctaaccgtt ccgataaagt attagctagt gaagcaccag acaaaatgtt
     3301 gaccaaagtg attcctactt ggattgtcga taggaaatta tttggattct gcaatatttt
     3361 taaaagcttt tgagatttca catctccttc atcagctttt tgctcgattc gattcttatt
     3421 gatcgaaaca acagcaattt cggaagctgc taaaaaagca ttgatcaatg tcaaaacgat
     3481 caataataaa atctgcgcta acagcgactg actctcaggg tcagcattca taatcgcatt
     3541 ctctccttat aaaattaatt tttagtttac gatagaaaaa aacgtggcac tatagccacg
     3601 ttaaaatggt atcacaaagc aattttcttt gcaataaaaa caagtgtact aaaactacct
     3661 acgaggtcgt agcaattttc ttcttgtttt cattttgctt gtcttctttg acgattttga
     3721 ttatatagga aatgatcgtc ttgcagattg cgtaaaaagg aacacctaag aagataccaa
     3781 gcaatccggc aatatttcca gctactagaa gaacgataat gatcgttaat ggatggattt
     3841 tcagtgattt cccaattaca ttaggataaa tgatattccc atccaactgt tggacgatta
     3901 gcacaaccac acaacaaagt aatgctttta ccggatcatt gaagactgtt actagaaatg
     3961 caggtgctag tcctagataa ggtcctaaat atggaattag gtttgtcaat cccgcaatga
     4021 ccccaaataa gaaagcataa tccacaccaa ttaccatata tcctaaaaat gtgaaggtac
     4081 caacgaataa acattcgatt gcttgtccgc taatgtaatt agccaatgtt ttattcagtt
     4141 gccccaataa ctccacaatt tggtttcttc ttttttctgg gaaaaactgt tggatgtttg
     4201 gcactagttt ttctccgtct ttcaacatat agaacaagat gaatggtgct gtaatgatta
     4261 ctacggttgc cgaagcgatc gttccaacga tcgaacctaa actattggat actccactaa
     4321 ggaattgctg aatgattgtc ccgtaagaaa tgtctaattg ctcaaaatat ttattgatat
     4381 ctacttcctt gaacaaaggc aattcggcta cctcttttaa ccagctttct acctgtttga
     4441 tgaaatcagg catattagat gccaatgagg aaatttgctg tactagactt gggatgacac
     4501 taagtaaaat ccaaataatt gctgcaacta aaaggagcag cacaattgcg acagcccata
     4561 ttcttttgat ttttgctttc atcaacagct tgacaatagg attcaataaa tagtaaagaa
     4621 agcctgcaat cagtacaggt gcaaataacg tggaaaaaaa tgtcccgatc ggttcgaaaa
     4681 taaagtttat ttctgatgaa atcatgatca atgcagctat gatcaataat tcaagcgacc
     4741 aaaacattag tttagactgt ctaagttttt caaacaatag atttcctcct ttctttctta
     4801 ctagtatata ttatattcgt taaaaattct tttattcttt ttttattttt gaaaacttgc
     4861 catacttttt atttgtttta ttttccagta tactatacga aaggaaggtg gctagaccat
     4921 gaaagttgta caaacaaaag atacaatgag caatatttac ttagatgctg tacggatcag
     4981 gcatcaagtt tttgtagtcg aacagggtgt gcctttgtct cgagaaatag acaaagatga
     5041 agcccattgt atccacttcg tcctgtactc agataaaaaa gaaccgcaag gaactgtacg
     5101 tcttttgcct ttagaaaacg gaaaaatgaa attacagcgg atggcgattc tttctgaata
     5161 cagacaccaa gggttaggaa aaatactgat tgaagaagca gaaaactttg ctaagaatca
     5221 aggctataat acgattttat taggggcaca gtccacagct gaaacttttt acgaaaaact
     5281 aggatataca gcttacggcg atccttttga agatgctggc atgcctcata tttatatgaa
     5341 aaagacttta taaaaattta tttcgaaaaa agcaaaaaac agcaagcttt tgagaaaaat
     5401 ctctcactgg cttactgttc ttttttgtgt ttacttacgc gacaattgct tgtaacggtt
     5461 ataccaaata tcaatatagc cttccgagaa cgggccatct ccattattga tccagtcaac
     5521 cagaatcttc acattttctt ttaggatgaa gtcaatatcg ttcggataat tcatcatttt
     5581 gctgtgcatc tcatattcat cgacatccaa tagtcgtttc tcgccgtcag gaaatacctt
     5641 gatatccaag tcatagtcga tatatttcaa ggcctcatcg tccagcaaaa aaggtgaagc
     5701 tagattacaa taataggaaa cccccttttc tctgatcatt gctattacat tgaaccagta
     5761 ttttttatga aaataaacaa tcgctggttc gcgcgttacc catcggcgtc catcagattc
     5821 agtgactaat gtgtgatcat tcacgccgat catagagtgt tcgcttgttt ttaataccat
     5881 ggtatctcgc cacgttcgat gcaatttgcc gtcgtgcttg taactttgga tcgtcacaaa
     5941 ctctccttct tttggaactc ccatagcaac ccttccttaa gcagcatttt gtgctttgtg
     6001 tttgggtgtt cattacaccg acatcataat tattatatca ataaaaatga gctttgccta
     6061 gaaaggattc ctattgtttc gatttttctc taactgatct accagtttca tttggacctt
     6121 aggaaaaacg accgaatcaa atgcatcagg cttcaaccac ttcgaagttt tgttctcggt
     6181 aaattcttgt tctgctccct cggttgcccg tccgtaaaaa aggagaacat gccatttcag
     6241 atgactaaaa atatgagtaa cttctcctaa atgtcgtgtc tgccaaacaa agagttcctg
     6301 cttttcaaat gggagatttt gctcttcttc tgctactagg tcatcaaaca gatcgatttc
     6361 tggctttgtt tcccattctt ttttcaatcg ctcatactcc tcttgtgtca cttccatcat
     6421 tggaaatgtc cacatattcg ccagcaattt ttggctatct ctttcttcaa aataatacgc
     6481 tccagaatga ttttgaagag ccgcactgat ataataaacg tcttttggct ttgctttttt
     6541 tgtttttaca ggaaaactcg tttggattcc tcgtttgtta gctaaacaaa aggcttggat
     6601 cggacaagtc tcacactttg gagaagttgg tgtacagata gctgaaccta gatccatcat
     6661 tgcttggttg aattcacctg gatgcttttc atcaatgatt ttccgcatcg cttcatcaaa
     6721 gattttcctg ctagaagctt ttgcgatgtc tgcttcaatg caaaacaatc tgctgactac
     6781 tcgcatcacg ttgccgtcaa ccgcgggttc tggaagtcca aatgcgatac ttgcaatcgc
     6841 gcctgtcgta tatggtccta ttcccttcaa tgagctaatt tcttcaggcg tttgcggcat
     6901 ttccccatca aattctgaca tgatctgttt agcagcagct tggatgttcc gggctcttga
     6961 gtaataacca aggccctccc atgcttttaa aagcttttct tctggagcat ttgctagctc
     7021 ttcaattgta ggaaaccatt ccatgaaacg ataaaaataa tcgatcactg tgtctactct
     7081 ggtttgctga agcatgatct cagagatcca gatacgataa ggatcacgat tatagcgcca
     7141 aggcaggttt cttttttctt gttcatacca ttgaatgaac tgatcttgaa attcttttgt
     7201 ttctttatct gtccattttt cccattcttt attcactttc ctcttccttt tccgtaatga
     7261 atcgctggat catatccaaa tcaaatcctt tttgatataa gctttgcttt acttttagat
     7321 ttcttttttt aggttcaaac cttaaatttc ttcgccatag tttatctcct tgtttgacta
     7381 aataatcata ttctatttct tgatctgctt cttgcggaag atcagccatc gcttgttgga
     7441 tcacttcttg agaaaaacct ttcgtcatca atgtttgttg gatttttctc aataattctt
     7501 tttggctttt tccgcgattt tttccaaacg ctttttcggc tgtctgatgt gcattttgaa
     7561 cttggtcttc aaatgtatat tggctgagcg cttgttcaat gatttcaggt ttcaaacctt
     7621 tttgctgcat ctgctgcgct aatttctttg gtcctttgtc acttaagcgc atattcgtgc
     7681 gcacataact ttccccatag accaaatcat cgacaaggtc caattctttt aagcgggcta
     7741 cgattttatt tcgatcttct tgaaggattt ccttttcttt taagtatgtt cggatttctt
     7801 tttccgatcg caactgataa cttaagtaat tcattgctag gtgaaaaccg aaatcagctc
     7861 cgctactctt cttgatctct tggatagttt cttcgtctaa ttcccgtcct tttaataatc
     7921 gatagcgtac taatacatct tcagatactt gtaaagcttc tccttcagat aaatctacgc
     7981 gatagaaagg gcctttgcct ttactgattc tcgtaacggt taacataaac atcctcctcg
     8041 aacatatatt ctctatcata ccacagaaaa tccccgtaat caggtattat atgttagaat
     8101 aaaccacaga aaaaagaaaa agggagatac ttatggcaga aacaattgtc aaaactggac
     8161 aaacggttcc tttgaaaatc aagcgtttag gcataaatgg tgaaggaatc ggttatttca
     8221 aacggttgat catatttgtt ccatatgcac taccaaaaga agaagtacta gtgaaaatca
     8281 cgaaagcaac tcctcgctat gcagaaggag aattaataaa agtcaaaaaa acaagcaaag
     8341 atcgcgtagt ggcaccatgt cctgtttact atgaatgcgg tggctgtcaa ttacaacact
     8401 tagcttatca agctcaattg gattttaaaa aggatctgct tttgcaagca ttggaaaaat
     8461 tcaaaccagc aggctttcga tcttatcaac ttttgccgac gatcgggatg gatgagcctt
     8521 ggcattatag aaacaaagca caatttcagt tacgaaaaaa taagcagact caaaaaattg
     8581 aagctggttt atacgaagca aactctcatc agttagtccc tataacagac tgcttggttc
     8641 aagaacctcg aacacaaaaa gtaatgaata ctgttgttca actattgaat aaatttgatg
     8701 cacccatcta tgatgaacgg acaaacagtg gaatcttccg cacaatcatg gttagaatcg
     8761 gtgttaaaac tggggagtta caagttgttt ttattactcg cagtaaaaaa ttcccgcaaa
     8821 aaaacgcaat gatccgtgag atcaatgaac gactgcccga agttgtttca atcatgcaaa
     8881 atgtccaggc acaaaaaacg tctctcgtga tgggggatga aacgatccac ttatggggaa
     8941 aagaagccat tgaagaacaa atcaatgaag tgactttcga tctttctcct cgtgccttct
     9001 ttcaactcaa tcctcagcag accgaattgc tttatggcga aggaatcaaa gcattagatg
     9061 tccagcctaa tgaaacgatc gtggatgctt attgcggagt tgggacgatc ggcttaagtg
     9121 tagcaaaaca agccaaagaa gtacggggaa tggataccgt tcctcaagca atcgctgatg
     9181 caaaaaaaaa tgcggaaaga ttaggatatc acaacactcg atatgaagtc gggacagcag
     9241 agcaagttct gcctaaatgg ttgaatgaag gttttcagcc tgatggtatc atcgtagatc
     9301 caccacgtac gggtttggat aacgcattga tccaatcatt attgaaatac cctccaaaaa
     9361 aacttgttta tatctcctgt aatgtttcta cacttgccag agatctggtt aatttaagta
     9421 aaaagttcaa gattgaatat ttacagtctg tcgacatgtt tcctcagact gcacgttgtg
     9481 aagttgtcgt gaagatgacg agactcgctg actaaatgaa agaggttgtg aaaaagtctg
     9541 ccctgtatca agtaataaga acagccaaac aaaaatagct cttcatgttt tatgaatacc
     9601 gtacttgtga ccgaagagtt aacaggagga cactatttat ttaaaaaaag ggttgtgaca
     9661 tagtttttgt cacagccctt tttgattatt attggatatt cacgtctatc acttgataaa
     9721 atgcatttgt cgtatcgctg atattccata ctgctaaaat cacattatat ccttttcgat
     9781 cctgcggaag tgtgatcgtt tggttgactg tttttggtgg aacagttgct ttgtcgtcta
     9841 tttttgtgat caattcaaaa tcactaaatt tcaaaggttg attaggattc caagttggtt
     9901 tggtaatgta gtaatcccaa gtacttgtcc gatgttgtgc tgttaaattc caagtaatat
     9961 ttaatgttcc tggattaacc atgtttttat gccatctgtt agatgtctgt tcatctaaag
    10021 gctcaaagcc gctgactcct gcactagcaa tttttccatc aatgaatgta tttttggtag
    10081 cttcgatact ttgcggttcc cattgtgctc ttccgacatt ttgatttaag tttcctgctg
    10141 ctgatgttcc taggtatgca cggcttcctg gagaagtaat ataaccatgc gcaaaacctg
    10201 gaatgctaaa accgaacaac cctaaactaa ctaacccaat aaacaataac gtactaatct
    10261 ttttcatgta gaaacctcct tcatttgata accacattgt aaacgcttta ttgtattaac
    10321 acaaacatta tgcttttata attttatgtt gaagtaaaaa aggtgaaaaa agtaagatgc
    10381 agattataac agaagtagac gagaaaagta caaatagaaa acatgagaac caaatcatat
    10441 gatctttttc aaaaacatca ttcatataaa caaatgttca actttttaat aacaacaatc
    10501 atcaattttt ttcttacttc acaaactaaa tacaggtctt gctgtgagcc ttcatggtaa
    10561 actactatta tagaaaggag ttagtaaaat gaagatgata aaagatattc gtttttgggt
    10621 ttgtgttata ggaattgtca tattaggctt tttatcaggg ttgcttagcg gaaatcccgg
    10681 agaatattac tattctttgc aacttccacc atttgcacca cctagctgga tatttgggcc
    10741 gatgtggacc ttactgtata tattgatggg tatttctctt tatttgttac ttaatcacaa
    10801 caacaaaaaa cagcgaaaca atcttgtcgg tctttttgtg attcagttca tttttaattt
    10861 tatatggtct gctctatttt ttaatttaag aaatattttc attgcagcaa tcgatattac
    10921 acttttagtt attttcttgt cagtgcttat gtatcagttg tggctacatc atcgcctggc
    10981 aatgtggctc atgattcctt attatttatg ggtactattc gcaactctat tgaattattc
    11041 aatttatttt ttaaactaag cgctaaaaag agacgtcctg taatttaaca catagaaaaa
    11101 gtgccaccta agttagattt tttgtctaat ttaggtggca ctctttatta accaaactat
    11161 ttttttctag tacccacttg ttgttccata ctcattattc tggtcataac cataaccatt
    11221 ttgttcctgt ccagcgtaag gatcataact ttgagtatct tgacttggtg tatagtactg
    11281 acttgtatct tctgtttgct gataataggt attcggatct tggtactgat catatgtgtt
    11341 ttctgaatag gaagaataat cggtgtaccc ttcactattc gatccatcac cgtttgtcga
    11401 atcgccatct tccgaatcat caaagaacat cattgaagca ttctcatttt tcaaattagg
    11461 taatgtatcg cttgttttta tatttaattg tttcttcagt tcattttgga tcgaaagaag
    11521 atcatttttt cctaaaattt gatagtagat atcattgatc atttgtcctt cgccttgcaa
    11581 ctggtcttgt ttgattgttt caaacgcagg ggtgtagttc gtcgctacat caagcatgtc
    11641 atcccaatct aagtccgtcg tcacattgtt ctcaactgct tttaaaattt ttctataatt
    11701 gcttacgcca tctaaactta aaactttatt gacgattttc gtcactactt cccgctgacg
    11761 acgttgtcga ccgatatcgc cttctggatc ttcgtgtcgc attcgagaat aggctaaacc
    11821 tttttctcca ttcaatgttt gttcacctac tggtacatgg acaccatcca aagtaaagtc
    11881 aattttatta ttcactttga tgccaccgac cgcattgatc aaatcttcca tgccatccaa
    11941 attgatcgtt acataatgat caataggaat atcgagcagt tgctcaattg aatccatggc
    12001 catttcgaca ccaccaaatg cataggcatg atttaattta tctaccgttc cataacctac
    12061 gatattcgta taaatatctc ggtccaaact gatgatcgtt gatttttttt gtttcggatt
    12121 aactgttact accatcatcg tatcagaacg tccttgttcc gttcttccta atccaccggt
    12181 atctaaacct aatagcaaaa ccgaaaaagg ttctttgtca tcaatgctta ctctctcaga
    12241 acgtttggag gatattcgat tagagttttt agaaattttg tttactgttt ggctcgcttc
    12301 tcccataaat ctgattccgt atgctgataa tccggcaatc agcaaaacaa atacccctaa
    12361 caaggttaat agaacttttt gccatcgctt cataatacac ctctgtttca cattttctaa
    12421 aatattgtct catataactg ttcaatcagc aaattcactt tttaatcata agaaaacttt
    12481 aaaaaacatt tttgtgtaac gattactatt ttttaaaatt tctcaaaatt cttaactatc
    12541 ataccatacc gtgaaaaaga tcgaaagacc gaatacacgc agttcgcaat cgttttatct
    12601 ttttttacgt attttttcca gaaaaagcga acaacaaggt attttccaaa taatcgtgca
    12661 gtaaaatgat cgcacatacc tttcccatct ttttaacagt ctttgcctac cgttcagatt
    12721 atcattcgat ttcacgaaaa tttcttccta tgactggtcc ttttgttaaa cccatcttct
    12781 attcgtttta tttccagctc actgcatggc tcttaaaatg actcgaacta gtgatttcat
    12841 taccatcatc ttttaatgga tagatatgct cctcttcaaa gtaaaacgat tttcttttaa
    12901 agtaatagaa ttgacatggt aatgagtatg tatactagtg gtttcacaaa tggtctttta
    12961 ttcaacatcc aaaaactgaa atttctttaa aatccattac taacgaagaa aatgttcctt
    13021 gcttgataat tagttaggac atgtcaagtg aacaaatgca tttacttagc caaaaaatct
    13081 aaaaacttca taaatagaca aaaagacacg actctctaag aagtcgtgtc tttattcaac
    13141 aaattattat aatttagtca cttcagcagc ttgaggtccg cgtgcacctt caacaatcgt
    13201 gaattctact tcttgacctt cttctaggct cttgaagccg tctccttgaa ttgctgagaa
    13261 atgaacaaat acgtcatctc cgccgtctac tgtaataaac ccaaatcctt tttcattgtt
    13321 gaaccattta accgttccgt gttccataaa tgtatagcct ccaaaaaagt tcttgcaagt
    13381 aaccttgcaa gaacattata cccaatttag aaaacgctag caagctttcg ctcttttatt
    13441 ttccaaatta tttttaagac tgatatgacg gaggaaatgg ttattttgct gcttcgaaac
    13501 gtttgtttac ttcatcccaa ttaataacat tccagaatgc tgcaatataa tctggtcgga
    13561 catttttata tttcaaataa taagcatgtt cccaaacgtc caaacctaga attggtgtct
    13621 ttccttccat caatggagaa tcttgatttg cagtagaggt aatagctaat tttccatttt
    13681 ccattacaag ccaagcccat ccagaaccaa atcgtccagc ggctgctttt ttgaattctt
    13741 ctttaaaaga agaaaaatca ccaaaagctt cattaatcgc ttcttttatt tctcctgtag
    13801 gttcgccacc agcatttggt gccatgattt cccagaaaaa tgaatggtta gcatgtccgc
    13861 caccattgtt acgtacagct gtcttgatat ctgttggaat agcgtccata tcagataata
    13921 attcttctat tgttttttcg cctaattctg ggtatttctc aattgctgca tttaaattcg
    13981 tcacataagt attgtgatgc ttatcatgat gcagatgcat cgtttcttcg tcaatataag
    14041 gttccaatgc gtcatatgca taaggtagat ctggtaatgt gtaagtcata aaaaaattcc
    14101 tcctcaatgt tttctttata atgtctacat tacaaagagt aacaaagaga aggaaattct
    14161 tcaattgtta tgcctagtcg tctttttcac tttgacctac ttcttcttca aaaacctcag
    14221 tttctttaaa aataaacaac ttgctgaaaa tatagttcgc taggataaca ataatttgag
    14281 tgatgatttt cgccacatac tcattgctgt tcataacatt gatcaacaat accattgccc
    14341 ccatgtccaa tccgtaagaa aggatacggt agaagaagaa cttaccgcat tcaataagtg
    14401 catgtgtaaa attcggtgat tttgaatgga atacccaaag tttgttcgtt acaaaagcaa
    14461 acaatacaga acaaaaccac gaaatagaat tggataaggc catacttaaa tcaaaatatt
    14521 ggcgtgcgag agcaaaacta acaaaattta caatcgtagc caaaccacca aaaaagagat
    14581 agactagtat ctcccaatat ccttttgctt ctaaatatcc ttttaactgt cgatactgct
    14641 tcatgagcca acctacctta ttaaaaattc ttatttatca tactctatat ttcaataact
    14701 ttcatcttta tttaaaaaaa ttaatttttt ttgatccttt gcctttgtct ctgcttggat
    14761 cgtcacatgt gtaataccat attttaattt taataaagaa attatacgag gatatatctt
    14821 ttcaatctcg cttagtagct gatcttcaaa atcaatatgt ccgtcgaaaa taattatctt
    14881 ttcgctatac tgccaaatat ggacatgatg aacattcttt accccatcga ttgcttcaat
    14941 ttcttgttcc attttttcat agtcaatagc aggtgcactt tgtatcaaaa tagcggcagc
    15001 ttttttgatc accattatcg cttctatcaa tatatatatc gaaatgatca gtgtaataac
    15061 tggatcgatc cagtagatat cgaaaaaacg gatcaaaatt gctccgataa tcacacttac
    15121 cgaagacagt gtatcactta acaaatgcaa ataactagat tttatattca aactttcatc
    15181 tgcttcttgt cgtaataata atactgagaa caaattcgcg agcaatccaa taactgctac
    15241 tgttagcatc aaatgactat ttatcttttc tggtgaagtg aaccgcttga atgcttcaac
    15301 ggttaaaaac aaggataata tgatcaatat gaatgagttg atgaaagcac tcaatatttc
    15361 taaacgctgg tagccatacg tacgtttttt atttgcttct tttcgggcca ttttttgtgc
    15421 cccgtaagaa aaaataattg ccaatgtatc actaaggtta tggaaagcat ctgaaacaag
    15481 agacaaactc cctgacataa tccctccaat aaattctgat aaagtgatca ccgcattcaa
    15541 aaccactact ccaaatagtt tttttgatga tctctgtggc tgtgtaggca ctattccttc
    15601 ctccatttta tttatattgg atagtcttta gacaagaata acattttttt gatatttcac
    15661 caatcattca gatgtttccc taaagcactt gtatttcact tgatacttcg ctagaattgc
    15721 aagtgatgac actactagat gaggagtact tatgacaaca aaacaattaa ttatcagttc
    15781 ctttatacga ttcaaagacc taaaggagac gagaaatgtc ccattttgga aagccattgt
    15841 ctatctttta gttttgagta taattatggc tttgccaatc tcttttcaag tttttcaagt
    15901 cttaaacaac attcaattgg atgggcaaaa gattgctact cgcattcctg attttacaat
    15961 ccaaaacggg cagcttcaaa ccgaagaaaa agaaggattc atttaccaaa caaactccat
    16021 tatcttcaca tttgatccag agggaaaacg aacagaacag gacatctcta ctgatttgat
    16081 ggggaatttt gttagtgtcg ggatgttaaa aaacaaactt atcatagctc ttcctaatac
    16141 aggcacgact tctgctttgt tgaacaataa ccaattagaa cttccctaca ccaatgaatc
    16201 tttaaaaaac ttaacaggta aggagctccg ttctttttta agtaaagcat ctataccact
    16261 ttggataaaa gtcctgacat ttatattttc tatttatcct agttttctga atctgctgat
    16321 cactttgcta tttaccaaca ttgctgcttt tctctatgca cgctttcgac taacaaaagc
    16381 tacttttctt gattgtttaa aaacattgat ctattcaatt tcattgccta ctataattgc
    16441 aacaatcttg atggtctttc ttccttcttt tgacacaagc gccttcattg cttttgcagg
    16501 aatctttatt tttgctcaag ctgtcaaagg atgggcaaaa atatcgatca attaagaaat
    16561 aaagacaact cgttatttct acttcaatag acccttttag aggttgtgac atttttttgt
    16621 cacagcccct tttttatttg aaaaatattt ctttctcaaa aaacaagcaa ggcgcttcaa
    16681 agttcttctt gtatgcgcta cacttagagt aaagaaaggc gggaaattaa ctatgtcaga
    16741 aagaatcatt tatgcagatc gtccatttcg tgatgaattt atcgaagaaa tccatactat
    16801 agcgcctggt tatatcttta aaacagaatt acaaccagag gatctacaaa atgtagagat
    16861 cagtttgggc tggaactcag actaccaaga aaaactatta gcctcttcta ccttgaaatg
    16921 ggtccaatct atctcagctg gtgttgatca gctacccctt gagacttttt caaagaaaca
    16981 tattttactt tcaaatggta gtggcatcca cagtgaatcg attacggagc atgtcttagg
    17041 aatcatcctt gggtattctc gtggtctttt tcaagcacaa aaagcacaga gtgacatgaa
    17101 atggttaggt tcatcggtcc attaccaatc tatccaagga aaaaaaatac taatcattgg
    17161 aactggccat attggaaaaa tgattgcaaa aaaatgcaaa gcattagaca tgaactgcta
    17221 tggaatcaat acatccggtc atccagcaga aggaatggaa aagacttttt ctttaaatca
    17281 actaaaggaa gttcttcctg aagtagattt cgtagtcaat atcttacctc taacggagga
    17341 aacaactggg ctctttgatc agcatatgtt tgaacacttt gatccaaaat ctgtattcat
    17401 caatgttggc cgtggtgctt ctgttaagac acaagatctg gtgcaggctt taaataacca
    17461 gcaactttcc tttgcagcat tagacgtttt tgaggaagag cctcttccac aagatcatcc
    17521 tctttggaaa atggacaatg tactgattac ttcccacatt gcaggtttga cacctgattt
    17581 tcaaaagaaa ttaatggcta tttttttaac caatctgaaa agctactttg ccacccatga
    17641 gcttcaaacc aatcaagtaa aattgtctgc aggatattaa tagctgaaaa caatattttc
    17701 aaaagaacaa agagaaggtg acaaaacttg tgtcaccctc tcttttttta ttgtttttta
    17761 aagtgatagg ttggctcctc caggttggga tcataatcaa caagtaaatc atatccttta
    17821 aagaaccatt catctgcttc ctcgataaaa aaaagtgtcc catccatttc cactttcttt
    17881 acaggatgtt ctggctgttc aactgacatt ccaatcgaaa aaccatcatg aacctctgtt
    17941 ttgccgtaga cttttccaaa aaaacgtatt ccgtaagttt ctggcagatc aatttctgag
    18001 tcaaaccact tttgtgcttt tggcgttacc gttaattcca tcccaacaac ctactttctc
    18061 ttttctttct attgattcat cttatcgtct agaatatcca catcgcctgt ctctccaatc
    18121 accagtagat tatcattttc gcgaacatat tcatctgctg caggagaagc gataaccgtt
    18181 tgatctactc ttcgtatggc gactactgtt aagtcaaaac gttgcctgaa atttaattca
    18241 gctaatgttt tgttgaaaaa tttaggattg gagactcgga tttctgctaa agaatattta
    18301 tctgacagct ctaagtagtc tagaatattc tttgatacta agttatgtgc aatccgtatc
    18361 cccatgtctc gctcagggtg tacgacacga tctgctccga tcttctctag tacacgggca
    18421 tggtactcat tttgggcttt tgctaaaata ttcggtacgc ccatttcctt taccatcaga
    18481 gtaactaaga tactggcttg gatatcttct ccaatagcga caattacatg atcgaaattt
    18541 cttataccta atgaacgaag tgtcatctca tcctgtgcat ttgccacaac agcatgagtg
    18601 gctatattca tatattcatt gactcgatct tcattactgt caatagccag tacttcttgt
    18661 ccagcttcga tcagtgtacg gcagatgctt ccgccaaatc gtccaaggcc tacaattgca
    18721 aaattttgtt tcaccatttt cctcctttag tatcttcttt tctcagtata acaaagcctt
    18781 tccaaaaaca aaagcaagca tcattattgt aaaaaagcca agaaaaataa tagttatttt
    18841 ctcagctttt gattatcctc tttatcatcg tttttatttc accaaaccat gtttaaacgc
    18901 atagatagct gcttgagttc ggtcttctac ctctaactta gctaatatat tcgacacatg
    18961 cgttttaact gttttcagtg tgataaacaa ttcatccgcg atttcctgat tacttttccc
    19021 ttcagaaatc agcatcaaaa tttcattttc tcgattcgtt aattcttcat gcaatactgg
    19081 atcatttcgc cgactcattt tttccatcat ttttgttgtc acttcaggtt ccaatactcg
    19141 ctcgccgcgt tgcgtagccc ttatagcatc agctatttca tgtgcggtag acgtcttcaa
    19201 caaatatcct gctgctcctg cttcaattgc aggataaact ttttcatcat caataaaact
    19261 tgtgacgata atgattttgg cttttggaca atcttttaag attgcttttg ttgattcgat
    19321 gccatccatt tcttccatga ctagatccat taaaatgaca tctggtcgaa gtgccatcgc
    19381 cttttcatag ccttgacgtc cattttcagc ttctccaatc acttcaatgt cttcttggat
    19441 agataaataa gaagagacgc ctaagcgtac catttcatgg tcatctacta ataaaacttt
    19501 tatcatcttt atctatgcct ccttcatcaa agggaccttg atctccacgc tcgtaccttg
    19561 ccctttaaaa ctaatgattt ttactgttcc accaatacca gctactcgtt ctttgatatt
    19621 gttcaatcca taacttcccg ttttggtttc attcatatta aatcctgtcc cgtcgtcaat
    19681 gatacgtaat aaaagattgt tatctatttt atgcaaatag acttctaatt cattagcctt
    19741 agcatgtctt aatgtgtttg ataacaattc ttgaacgatt cggaacaagt gatcctcaat
    19801 ggaactagtt aattttacat cttctacatc ccattttagc gaaatctgga ttttattctg
    19861 caattccttc aataactgct caatgccttg ttttaaactt ttttcttcta aattgactgg
    19921 acgcagatga agaagcagcg cacgcatctc agactgggaa gcgttgatga tctctgccac
    19981 cattttcaac tgcttacgga acatttctgg tgtctcactt ttttctgctt gctccgtcaa
    20041 tgccgacatc atcatcatcg ctgcaaataa ttgctgtgaa actgaatcat gaagctcacg
    20101 agctaatcga tgcctttcta attctaaaat ctcttctttg gtttgtccat ctacatattg
    20161 aggacggcta gtcataatct gtaattcact ggatacttcg atcatttttt cttttatttt
    20221 agcaatttcc atatcaatat ctttgatgta cagatcatcc gacgcgcttg ggatcggctg
    20281 atgcaaaagt ttggattcat aattgccaga agaaagtgca cgaagctttt cttcgatttt
    20341 cccgtatttt gccttttgaa taagtgataa caatagaaat acgatcaatc ctacacctaa
    20401 tgaaattgcc atcaaatgga agataagcgg aacgtataat aaacgcgcac gcagcaattc
    20461 tccccaccaa tgactttggt tactggcata aaaataagtg aaaagtgaaa atagaataat
    20521 aaggaaagcc gcaatccccg aataaacagc tagcattgct ttggatattc ttcccatcat
    20581 attcgtatca cctcgacatc tccaaccaag gtattggtaa tgatcttcaa tctgcgagga
    20641 ttttcatcat aatcactgct gcagattttg atctgttcgt tttttaagga aaactgctct
    20701 tcttcaaaca agacatttcc tactaatgtc gcatgctcta aacgaatagc cactcccagg
    20761 ggtactaaga tccgtgttct gcctatccct tttcttacaa tcacaatatt atcatctttt
    20821 ggcaaaagtg tattacccaa atcgatgata gtatcaccag aaatgatggc aatgttgata
    20881 tcatcccatt catagacgtc gtttccaata cgttggttac caaataattg ctgtctttta
    20941 cgctcattat tatgtgtttt taattgctct gtttggacca tcatgatctg tttttttcgc
    21001 caaaaagcat tttttgtcaa atctattcct gaaatctcta cacctttcag cccgataaat
    21061 aaaacggcca atacaaccat catccataaa gcaggactgt tgactaagct aaaaaaaatg
    21121 accaagctcc ctaagatcaa ttgaaaattt tgaaatttag tacgaggata tttgcgcata
    21181 gctagatata tattgaaaat accgaatatt actaaaagca aaagtccagt attgtttacg
    21241 atttgccaaa cagcaaataa cagcagcagt gcttcgacta ccacaaaaaa acgccaagaa
    21301 ctattcatcg ctttttctcc ctcctgacac attctcattt catcatacct atttttttga
    21361 ctgttggcta taggacttta gatgtaagat cgttcagtga aacctgcaat actcaaagag
    21421 aaaagatcta gagtgcgtca tcagtaagat gaactctcta gatctttcag tcagactacg
    21481 cttgttctac tttgacgatt ttaactaaca tatcgccacc tggtgtagca atggttactt
    21541 catcattttt acgtttgcca atcaatgctt gcgcgatagg agaatcattg gaaatctttc
    21601 cagaaaacgg gtctgcttca gcacttccga caatcgtgta ctcttcttca tctccatcag
    21661 gcaactcgat gaacgtaact gttttaccga tcgatacctc atctgcatct acgccgtcat
    21721 tatcgatgat ttgtgcaaaa cggatcatat tttcaattgt tgtgattctt ccttcaacaa
    21781 atgcttgttc atctttagct gattcgtatt cggagttttc ggaaagatct ccgaaaccgc
    21841 gagcgatttt gattcgttca atgatttctc cgcgtttaac tgtttttagt tcttctaatt
    21901 ctttttctaa tttttcttta ccttctagag tcattggata aactttctct accatgtgta
    21961 acgtctcctt ttttcgataa aataccgcaa aaggtaatct tttgaggaat atagtccaaa
    22021 aaagattacc acgcttttct taaaatgtaa atgtttttct aaaaatgatc aatgactagc
    22081 ccctgttttt ctaagaatta tttacatatt tttgcactag ttccatatgt tcttcatacg
    22141 ttttagcgaa ataaacattt ccagtgctcg tatctgctac gaaataataa tagtcatttt
    22201 ctgctggttc taatactgcc ttgattgcct tttcgcttgg attgtcaaaa ggaccaggcc
    22261 catagccggt attggtatac aaattgtatg gtgaatctac agctgtatct tcatacgtca
    22321 ccaattcttt atgttctcct aatgcatata aaacagagat atctgactga agtggcatat
    22381 ttgccttgat tcggttaaag aagacttgtg caatattttt acgatcattt tcttttacgc
    22441 cttctttttc aactagagaa gccaaagtta aaacttcttg tacagtcaaa tttttctgtt
    22501 tgatagctga ataatagttt tgcatgaccg tatttgtctt gttcaccatc tcaatcacaa
    22561 gatccttaag cgaagtactc gtgtagtaat cataagtagc tgggaataag tagccttcca
    22621 aacggtattt tgtgttctca gctttagagg cactttccaa caattctggg aatttttgat
    22681 acaactcgtt aaagaaagtc tcatcattta ctaaatctaa aaattctttc ttgtctttac
    22741 ctgttacctt cgctacccgc tcagcaatct gatcgatgtc gtagccttct ggaatggcaa
    22801 ttttcccatc tgcaactttt gttggctcag atgtgccgcc ttcttgcaac tgcttgccaa
    22861 tctcatcaag agtcatatta ggcgctaact gataataacc agcttgaaag ccagttaagt
    22921 ttttaaattt tgtataataa ttgaaaacaa tcccactttt aataatattg tctttctcca
    22981 aaatttcgcc aatctgctta ttagaagagc cgtttgggat ttccacttgg actaattggt
    23041 cgtccgattt atctaaaggc ttcaacccgc tatctacata acgatatacg gtaaacccga
    23101 gaaatccgcc aataatcaat aatgtaagag cgatcactaa aatgatcttc ctcacgatct
    23161 tatcttcttt tttacgcaga tttctccgct tgtcgattat atctgctgta gctgccttac
    23221 tggcttcttt tttgtctttg cttgggtgtt gtttgtttcc tgacaaaaca actcctcctt
    23281 actatcatct tcgtaactct gtttattata catgaaattt ctactgccgt gaattatttt
    23341 accaaatatt atgtcaaaat tgtaagtggc aaatgctctt tttgaggatt acaatgaaaa
    23401 atagttaaaa atggatagaa aggatttaat atggcaaaag gaaaacatca aaaaaaacag
    23461 cagatctctc tttcttttct cgcagttttg ttgctggctg tcagcttctt ttcaacaggc
    23521 atcattacag gatggcaatt aacaaaacaa cctcaaccac aaatttataa aacggaaccc
    23581 gcacaacttg cttccaattt aacatctaat gagcagatgc ttgccagcaa aattgatcaa
    23641 ttactgcaaa aatcagatta tatcggttcg atttatgtac gcaaaaataa tcgagtgata
    23701 ttagaaaaag gatatgggta cgcaaatcta cgcataaaag aatcgaattc tccttctctt
    23761 tactatcaaa tcggttcgat ccaaaaagcg atgactgctt tacttatcct aaaacaaatt
    23821 caatctggca agataacttt tgacacaaag ctatctgaat tttatcctca gattcctgga
    23881 agtcagcaaa tctcaatcaa aaatatgctt tacatgcgct ctggtcttca tcgaaccgct
    23941 tctccaaaaa aacctatgtc agatagtgag atcattcagt ttgcccttca tcatttgaaa
    24001 tttactggtt atgatacgta tcattatgaa ccattaaatt atacattgct tacaggggta
    24061 ttgatcaaac taatgaatca gccttatgaa actcttttaa aaaaagaaat cattcggcca
    24121 ctccatctag aacatacaga tttttatgaa aatgtaaaaa attcccctca gcatgctgta
    24181 tcttaccaaa tgtctgcaac tgacgattat tcaaaagcat tggtcgagcc tgaaacagac
    24241 atccgaaatg aattaggcac aggaaatata agtatgtctg tttatgactt gaacaaattc
    24301 tttaccagtg ttctgtctgg cgatattata ccaaaagaac tacttttctc attgtggcaa
    24361 aataatggtg ataaacatcc ttacagcggc ggtgtttaca gtggaaatga ttatatcctt
    24421 gctcagggaa atatcaatcg attccatgca gtagcggcga tgaaaaaaga cacaaaagat
    24481 gcaatcgtca tggaaagtaa tatccaatct gataaaaaca tcaaattgcc ggccactgat
    24541 ttaagaaatc agatttacga actgatggaa ggtgttaatt taaaaagcta agtcgagaca
    24601 aaaaaaccag caggatgctg gttttttgtt tcaatcgatt tctgtcttta aataacgttc
    24661 cattccgttc aatatatcaa atttgggttg ataacctaaa gaacggatct ttgaaatatc
    24721 cgctaaagaa tcacgaatat cccctgaacg ctccgcttca tatttaagcg ctagctctgt
    24781 tcctaagatt tgatcaattg aatgaatcaa ttcaagtagc gtagtgcttt ttcctgtacc
    24841 gacattgaat tgctgtccta aagctttttc ttccttagca acaagtaaaa gtgcttgtat
    24901 aacatcttct atataaacga agtctctaga ttggctacca tcaccaaata aagtaaattc
    24961 tgttttttct cctgccaatt gttttttgta tcggtccact agaatcgaaa taactccgct
    25021 atatggagag ttaggatttt gatttggacc ataaacgtta aaaaatcgga cagcactagt
    25081 cggaacatca tataaatgac agtaatccaa aacatattgt tctgatgcaa acttatcgat
    25141 ggcatatggt gttaaaggac ggatgaccga ttcttctttt tttggcaacg ttggttcatc
    25201 gccataaact gcagctgatg aactaaatac caatcgtttt aaaccagatt gatattttcg
    25261 taccaattca agcaataaaa gcgcactgtc aaaatttaca cgatgagttt ctagaggacg
    25321 ttctacagaa tctgccacac tagctacagc agctaaatga aaaatatagg caaactgata
    25381 ctgtttcatg attctttcca tcaattgagg atctgataca tccccctcaa tgaaagtcac
    25441 ttgtttctcc atatccaagt tttctttttt tcccattgat aaatcgtcga ctactacgat
    25501 tttgttttct tttcccaaat aattcgctag cgttgaaccg ataaaaccag ctccaccagt
    25561 aatcaatata gtatccatca aattcactcc aattatgcat tctcatactt aataaagtta
    25621 gtttaccatt tttatattgt aaaaactata aaatttgttg aagttgggat aactagtttt
    25681 cttttctact ttatatacct aaaaatagtt tcaattagtt actccttctt ctttagatat
    25741 agttttttgg atagaaccca gatatcccca aaaaataaat gaacctatcg tactaaccaa
    25801 aatgagttct gtgataaata atgccgaaac tgctattgta agaatcatta aaatagcaaa
    25861 aaagtgctta tcttttacta gatttggttg tgtgaaacaa taacgcaaag ttttaaagca
    25921 ttggataaat aagaaggtta acaatgtcaa tagacctaat cctcctgtag cagccataac
    25981 taagaaaata aaattatgag cagttaaagt atcttttccc attaatgtat atggtagttt
    26041 atcatgtgcg tattcaatat agtatcctgg agaaccgccg gatatcggct tactcttgaa
    26101 tatttcaatt gcacttttcc aaagtccaaa ccttaaatta gaaacatccc cgccatcagt
    26161 gtcagggcgt tttaaatcaa ctttattgct gctagttgtt cctgatgaat cagatatatc
    26221 tttttttatt tctgtaatat gcggaacatg tgcccatgct tgagcagtta tatctgttac
    26281 ataatacatc gcaattatac ttagcaatcc tactactaat gcagttagcc atctttttaa
    26341 agtattagtt ttaagaaaac gactactata acttgtaaag aaactaaaaa caaaagttgc
    26401 tattaataaa caaactaatg tggtgcgtga gcccgataaa gcaataaacg agaattgaat
    26461 aaaaatatta attgcaaaaa atattttaaa tagaagattt aaatgcttgt aagtgaccaa
    26521 ataatatata gcaataatga tgacaattaa tgatataacc gcaccaaaat ttggatctgt
    26581 atatacacca aataaacggt tgcctaacca ccctaaacga actgggtaat acttatattt
    26641 tattggaatt tttatgccat attgaatgac aaaccagtat aatgatacta aattagctaa
    26701 aaaccatacc aatgaaagta tccaagtaaa tactttgaaa aaaaagttac tactgtagtc
    26761 tttgccaaac tgataaatca taaagaaaaa gattaattgc cacatgatta acttgatatt
    26821 ttcgctatat ccatatttta aattcattaa actgcttact agcatcgcgc caataaaagc
    26881 aattaatagc caatcatatt ttttaagatt tttttctcta aatgcaataa ttgcatctat
    26941 aatgatcaag acaactcccg ctattccagc aggccaaaaa agtaaagtat catatctagt
    27001 aggtaatagt aaatacgggc ctattgctcg taacattaga ataaaaccaa aataaataaa
    27061 ataccaacct ttcacatatt ttaaaaattt ttcattcaac atttttcctc catccaccta
    27121 tttctcataa acatttctta tcctttttag gataaactgg ttctttcaat tctaacaaaa
    27181 aaatgctaaa ctaacaatgt atattaatga atttcgagag gtgaaagatg aaaagagtaa
    27241 ttacttacgg tacctttgat ttgcttcact acgggcatat caatttactg aaacgagcaa
    27301 aacaatatgg agattatctt attgttgccc tttctacaga tgaattcaat tggaatgcga
    27361 aacaaaaaaa atgttatttt agttatgaaa aaagaaaaca gttacttgaa gctattcgct
    27421 acgttgattt agttattcct gaagaaaatt gggaacaaaa agtaaaagat ataaaagaat
    27481 accatgcaga ttatttcgtg atgggcgatg attgggctgg acaatttgat gaacttgaac
    27541 aactaacaga tgcacaagta atttatttac caagaacacc tg
//
LOCUS       DAZUMM010000032        26549 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000032 DAZUMM010000000
VERSION     DAZUMM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.032"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..873)
                     /locus_tag="ACG8E6_001304"
     CDS             complement(136..873)
                     /locus_tag="ACG8E6_001304"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5061731.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(940..1509)
                     /locus_tag="ACG8E6_001305"
     CDS             complement(940..1509)
                     /locus_tag="ACG8E6_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFS5061732.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1637..2509
                     /locus_tag="ACG8E6_001306"
     CDS             1637..2509
                     /locus_tag="ACG8E6_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5061733.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            2851..3723
                     /locus_tag="ACG8E6_001307"
     CDS             2851..3723
                     /locus_tag="ACG8E6_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5061734.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            3720..5678
                     /gene="nagE"
                     /locus_tag="ACG8E6_001308"
     CDS             3720..5678
                     /gene="nagE"
                     /locus_tag="ACG8E6_001308"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFS5061735.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(5929..6780)
                     /locus_tag="ACG8E6_001309"
     CDS             complement(5929..6780)
                     /locus_tag="ACG8E6_001309"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5061736.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            6903..7370
                     /locus_tag="ACG8E6_001310"
     CDS             6903..7370
                     /locus_tag="ACG8E6_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFS5061737.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(7633..8115)
                     /locus_tag="ACG8E6_001311"
     CDS             complement(7633..8115)
                     /locus_tag="ACG8E6_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS5061738.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(8215..9078)
                     /locus_tag="ACG8E6_001312"
     CDS             complement(8215..9078)
                     /locus_tag="ACG8E6_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS5061739.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            9676..10524
                     /locus_tag="ACG8E6_001313"
     CDS             9676..10524
                     /locus_tag="ACG8E6_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFS5061740.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(10695..11036)
                     /locus_tag="ACG8E6_001314"
     CDS             complement(10695..11036)
                     /locus_tag="ACG8E6_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFS5061741.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            11122..11829
                     /locus_tag="ACG8E6_001315"
     CDS             11122..11829
                     /locus_tag="ACG8E6_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS5061742.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            11816..12139
                     /locus_tag="ACG8E6_001316"
     CDS             11816..12139
                     /locus_tag="ACG8E6_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS5061743.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(12228..13265)
                     /locus_tag="ACG8E6_001317"
     CDS             complement(12228..13265)
                     /locus_tag="ACG8E6_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5061744.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(13712..14695)
                     /locus_tag="ACG8E6_001318"
     CDS             complement(13712..14695)
                     /locus_tag="ACG8E6_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFS5061745.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(15111..15881)
                     /locus_tag="ACG8E6_001319"
     CDS             complement(15111..15881)
                     /locus_tag="ACG8E6_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5061746.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(15874..16809)
                     /locus_tag="ACG8E6_001320"
     CDS             complement(15874..16809)
                     /locus_tag="ACG8E6_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5061747.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(17403..19052)
                     /locus_tag="ACG8E6_001321"
     CDS             complement(17403..19052)
                     /locus_tag="ACG8E6_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS5061748.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(19247..20143)
                     /locus_tag="ACG8E6_001322"
     CDS             complement(19247..20143)
                     /locus_tag="ACG8E6_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5061749.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20136..20816)
                     /locus_tag="ACG8E6_001323"
     CDS             complement(20136..20816)
                     /locus_tag="ACG8E6_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5061750.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(20817..21599)
                     /locus_tag="ACG8E6_001324"
     CDS             complement(20817..21599)
                     /locus_tag="ACG8E6_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5061751.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(21592..22509)
                     /locus_tag="ACG8E6_001325"
     CDS             complement(21592..22509)
                     /locus_tag="ACG8E6_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5061752.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(22512..22796)
                     /locus_tag="ACG8E6_001326"
     CDS             complement(22512..22796)
                     /locus_tag="ACG8E6_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061753.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(23497..24117)
                     /locus_tag="ACG8E6_001327"
     CDS             complement(23497..24117)
                     /locus_tag="ACG8E6_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061754.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            complement(24198..24608)
                     /locus_tag="ACG8E6_001328"
     CDS             complement(24198..24608)
                     /locus_tag="ACG8E6_001328"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061755.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            24638..25816
                     /locus_tag="ACG8E6_001329"
                     /pseudo
     CDS             24638..25816
                     /locus_tag="ACG8E6_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            25993..26279
                     /locus_tag="ACG8E6_001330"
                     /pseudo
     CDS             25993..26279
                     /locus_tag="ACG8E6_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7959 a         5520 c         4636 g         8434 t
ORIGIN      
        1 tctttttgat aaatttgctt tttctacata aaaagacgta aatgaagaat attcttctcc
       61 attcacgcct taaattgatg gtcgtttgaa taactattta acaagtaatt ctctttggat
      121 attatccgtt atggattata cttcctgtgt cgtcggacgg ataatcatgt tactgacaga
      181 catccggtct ggcgtttcta tcgcaaatgc tacagcagaa gctatatctt cggaggtcaa
      241 tccccattct ttttgcgctt catgaagttc aagagcagcc tttttgtcgc tgatggtttg
      301 atataattca gtttgaacag ctcctggaga gatgatcgta gatttgatgt tattttgccg
      361 ttgctcttga cgtaatcctt ccatgattgc acggacagca aattttgttc cgcaataaac
      421 ggcggaatca ggataaacaa catgtcccgc cacagaatca gttgaaatga tatgcccaga
      481 tttttgtttt tccatgattg gaagtacggc ggaaattcca tttaacacac ccatgatgtt
      541 gatatcaagc atattttgcc attcatcacg gtgaccttca actaatggag cggttggcat
      601 gattcctgca ttattgtaca atacatcgat tcgaccgaac ttttccatcg ctaaatcgat
      661 aactgcttgg acttgtgcaa aatctcttac atctgctgtt tgaatatata gctcagcttc
      721 tggtaaagat tcttttattg cttttaggcg atcttctcgg cgtgcagcta taactagttt
      781 agcccctttt tctgctaata gcttcgttgt tgcttcaccg attccgcttg aagcgcccat
      841 gatgacaatc actttttctg ataaatcatt cattctattc tcctcctgtt ttgaaaaatc
      901 tcttgcttat actcactatc tcttatttca tcttactgtt caaataatcc gttcgacagg
      961 tttgacgatt ttggcaggga ttcctgcaac gatggtattg tctgggacat ctttcgtaac
     1021 agttgcattc gctgcaacaa tggcattctc gccaatcgtt acgcctggca gaatagttgc
     1081 tcctgctccg atccaagcat ttttcttgat gtggattggc tttactgaca agcctcttcg
     1141 ttcttttgga tcggttagat ggttcaccgt gatgaggttg acacgaggac caatcaatac
     1201 tgaatcttct aaacagatgc cacctaagtc aacgaacatc acttgacgat tgataaagac
     1261 atttttccct atactaatat gtttgccgaa atctgtataa aaaggcagca gaacagtcac
     1321 cgtatcatct actttgtcac taatgatatt ttctagccgt ttgcggatat ctgctggttc
     1381 atggtaccca gtattcagcg ctgctaaagg ctcttcgttt tcagcttgga tctgatggat
     1441 cacttcaaag agcggatccg ttactcgaat ctctttattg acgatttgtt tttcgatgag
     1501 gcttgtcatt ttgctccctc cttttatcct tactttataa cggacggctt atcatgtcta
     1561 atacttataa aacatactta actattcctt ttaactatac ttagtggtat gcttttttaa
     1621 aaacaggagg gaaaaaatgg atattcgtat actgacgtat tttttagcag tagctagaga
     1681 aaaaaatatc agccgagcag caaaatctct gcatatcacg caacctactc tctcaaaaca
     1741 actgaaagat ttagaagaag aacttggtgt gacacttttc actcggggct cccgagagat
     1801 acagttgacg gattctggtc gatatcttta ttcaaaagga aaagaaatct tgtcgttggt
     1861 agaaaaaacg gcgaacaatt taacaaatga cgcaatcatc tctggagaaa tctacattgg
     1921 cggaggcgaa actaaagcaa tctctttcat ttctgatagc cttcattcgt tgatcaaggc
     1981 gcatcctgat atccagtttc atttatatag cgggaatgca gatgatatca tagacaaact
     2041 ggatagcgga ttacttgatt ttggactcgt aatcgaaccg acaaacaaac aaaaatacca
     2101 gtcatttcga ctgccagccc aagatcgttg gggacttttg atacatcgaa atcatccttt
     2161 agcaaagcaa accgtcatcc atccgcagga actgaaaact gttcctttga tcatttcccg
     2221 gcaaacgtca gtggacagtc aactagctga atggctaggt ttcagcatag atcagttatc
     2281 gatcgtcgga acatataact tattgtacaa tgcttctttg atggtcgata acgggacttt
     2341 tggtgcatta gctctagatg ggatcatcaa tacttatggg acggaaaaaa tattcatccc
     2401 actcgatccg ccattacaag cttcactgaa tctcatttgg aaaaaagacc agcctctttc
     2461 tcctgctgct cgtaaatttt tagatgtcct aacggagaaa tacagttaag tagagttttt
     2521 ttccttcgct ttcagctttt taacaacgaa taagataagc aaaagatcca acgattaaat
     2581 aacataagtt tcttaattta tttcttcctt ttatttctga taattccagt aaattactat
     2641 aattataaaa aaagaattga caaaacataa ctgtctatat aaaattgatt acgttaacaa
     2701 taattcgttt catgtgacta gatcatacta ggcatgaaaa atctctgagg aacatggttt
     2761 ccctaggagg tttttcgtgt ctatttttgt ttggccgcaa tcaactggtc tagcaaacgt
     2821 gaaaaccaaa tcttcaaaaa gggtgcgcgc atgaaaatca aaaagatctt taatcaaaat
     2881 gcagtattgg tagatgacgg tggacaggaa aaagtagcta ttggaaaagg gattggattt
     2941 gacagaaaaa gaaatgattt gatttttgat cgtgatgtcg aacggatatt tgtcatggaa
     3001 ccagaaggtc aaatcaagtt gcaaaattta cttaatcaga ttgatgaacg ctttttattt
     3061 gcagcagaaa cgatcattga tcatgcggaa actgttttga tggaaaagtt gaatgaacat
     3121 gtattgatcg ctttgaccga ccacattgct ttttctgcgg agaacattaa taacggtatc
     3181 gtcatccgca acaaactttt aagagaaatc gaagtgcttt acagtgatga attcagtatt
     3241 gctcaatggg cagtagagta tctgacaaaa gagcttggga ttccatatac ttatgacgaa
     3301 gcaggatata tcgcaatcca tattcacagc ggacgaagcg gtcggaacaa taaccatcgc
     3361 agtatccgtg aagtgacgat tgtatctgac atcatccatt tagtggaatc agaattggaa
     3421 acagatatcc attcagaacc attttcactg aattattcaa gactggtgaa tcatttacga
     3481 ctgcttctac aacgaaccca tgcgcaacaa tatgctgttt tagacacgga aatcgtagaa
     3541 atggtcaaac gaaaataccc agaaagctat aagatttcta aaaaaatccg tgtgttgctg
     3601 accaaagaat accagctcgc gatcacgaaa gaagaactgg gttacttggc catccatatc
     3661 gagcgtttaa gaagcgcaat cgtacaaaca aatgttaaca atcatgagga ggaaaaaaat
     3721 tgaaagcata tctacaacga atgggacgtt cccttcaact tccagttgcg gtcttacctg
     3781 ctgctgcttt actagtaggg atcggaaact ggtgggcatc ttatagtaac gacattgtcg
     3841 ctcatttttt acaagctggc ggtaacgcag ttttaggtca attacctttg ctgtttgccg
     3901 ttgggttagc actagggatg tcagtggaca aatcaggtgc tgctgccttg gcaggtttgg
     3961 tcgcatttga agttccagta aatgttttaa aaactgattc agtagctact ttattgaata
     4021 tcaaaccaga atcagttgat ccatcttttg ctcagatcag taacgtattt attggtatta
     4081 ttgccggatt gattgcggca gctctttata atcgtttcca cgaagcaaaa ttaccgatgg
     4141 ctttatcttt ctttagcggc aagcgtctag tgccaatctt ggctgccttt gtcatgttgc
     4201 tgatcagtgc agtgctactg ctcgtatggc ctccggttta ctctgcttta gtttcttttg
     4261 ctaaattcat tttgagttta ggctggggtg gtgcaggtct ttatggcttc ttcaaccgtt
     4321 tactgatccc tacaggattg catcaagcaa tgaactccgt gttctggttt gacgtagcag
     4381 gaatcaatga tattggtaaa tttttggctt ctgaaggaac aaaaggcgta acaggaatgt
     4441 atcaagctgg atttttccca atcatgatgt tcgggcttcc agcaggagca tatgctattt
     4501 acagaaatgc acgtccagaa aacaaagcaa aaacagcttc cttgatgcta gcagcagctt
     4561 ttgcttcatt ctttactggt gtgacagaac cacttgaatt ttcatttatg ttcgttgctt
     4621 ggccattata tgtcttgcat gcaattttca ctgggatttc tttagcagtg agtgcattct
     4681 tccattggac agcaggattt gcctttagtg ccggatttgt cgattacttc ctatctttga
     4741 aaaatccgat tgcgaatcag ccattgatgt tgatcgtcca aggattagta acagcagtga
     4801 tttactactt tggctttaac tttgctatca aaaaattcca tctgatgact cctggccgcg
     4861 aggaagcaga tctttcagat gacgatactg caacgactaa ttcagacaat aaatatgcgg
     4921 cacaagcaag caaaatctac gctgcattag gcggagccga caatgtaact tcgatcgata
     4981 actgtactac acgccttcgt ttgcaagtca aagatacagg cctgatcgat caaaataaaa
     5041 tcaaagccac tggcgtacct ggtatgaaga tcatcgacgg aaaaaatgcg caagtcatcg
     5101 taggtacaga agtacaattc gttgcagatg agatggcaaa actacatggt ggtgctgcag
     5161 cacgacctgc acaaacaaac accgtggtta aaacagaaac atttgccggt gaaacgatcg
     5221 aacaagatat ttacgctatc gcaaatggga aactgattcc aatcactgaa gtttcagatg
     5281 atgtattcgc ggaaaaaatg atgggtgacg gctatgctgt attaccagaa aatggagaaa
     5341 tcttctctcc aatcgctggt acgatcacaa acattttccc aacgaaacat gctgtcggaa
     5401 tccaaacaga tgctggtatc gaagtcttgc ttcatatggg catcaacact gttgacttaa
     5461 aaggcgaacc attcacttta tatgtagaag aaggtcaaaa agtcgcaaga ggtcaattga
     5521 ttgctttagt tgatcttgcc gctattcaat ctgcaggcaa aaacacggat atggtcgttg
     5581 tattcacaaa tggcgacaaa gtccaaagct tagaaatcga acccgcacgt gatgtaaaag
     5641 caaatgataa aatcggctct gtttccaaca aagcctgagc catcgtcttc cccagacttt
     5701 tttatcgata aacaaacagg agctgagaca aaaatcttgt ctcagctcct ttttccgaat
     5761 aaaaacggcg ttctcctgcc aactccgcgg taataagtcc agctaaacga aacatatgaa
     5821 gagctatttt ttatttgact cttcttatta cttgatgcag aacagctttt tcacaaaatt
     5881 tttcttcata ctcaaaacta atcggctttt tcacaacctc tttatacatt attcctgatc
     5941 caatatttct tttaatgctg cttcaagaga agttaatggt tttcccaata ctttttctaa
     6001 atcatcagag gaaatagcta actgatcctt tttaatatca tgttggatcg ctaaaaacat
     6061 gtcagctgct tcttgaggga atccggcttc ttttagattt tcgacaaacg ctgcatcact
     6121 tgcttcaata atttctaact ctttacctgt cgcacgctct aatgccttgg ctaattcttc
     6181 atagtcagtt agattgccgg ataattctaa gatttctgga gagtcacttt ctactagtac
     6241 ttttgctgca gcttctgcat attcacgttt caatgcccaa cctgtttttc catttcctgc
     6301 agcataaacg aattgaccag tttttaaagc ctgcccaatg ataggtaatt cattctctac
     6361 ataccaatta ttccgtaaaa atgtatggga aatacctgaa tccttaatca ttttttcagt
     6421 gaattggtga tctggcgcta atacactgat cgagtgatcg gcatctgcaa aacttgtata
     6481 agcgatataa gataccccag ctgcttgtgc tgccttcact acattcttat gttcggcttg
     6541 acgatttcct ggaacgccag agacaaaaag caatcgatct attcctttca acgcttcttt
     6601 cattgagtct aaatcatcat aatcaccaat acgtatatta aatccagctt ctttcagtgc
     6661 ttctcctttt tcttcactac gtactagtac atagatttca ttttgtggaa ccatcttttt
     6721 taagaattcc agcgcataac caccaaaacc tccagttgct cctgttacta aataatgcat
     6781 atttgccact ccctattctt tttatttgtt aagatttaca gctgtacatt tataaattac
     6841 acctttatca taaagagtgt ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac
     6901 tgatgaagta ttctctgcaa tttagtgatg caatccatgt acttgcctat attgaaatgt
     6961 ttcaagatac ccgatcactt tctagtgaga tgattgccag tagtatcgaa acaaatgcag
     7021 caaatgtacg taaaatcatg agtcagctca aaaaatccgg tttgatcttt acaaaaaccg
     7081 gaaaaccgca gccttccctt gccaaaagtc cagatcagat ttctttacta gatgtctatc
     7141 gcagcatcga aggtaataca cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg
     7201 tcgttggtgc gaatatccaa gaagctttag aagaaagcta cgctcggctg cagaaaaagg
     7261 cagaagaaga aatggctaag atcacacttg atagtctgat acataaaata gccgttttgg
     7321 aaaaagaaaa aagaccagaa aacacaggcc tcgtagagag atttttataa ttctctctac
     7381 tgtgcctgca tttttctgat cggaatcgtc tacataaatt cttttattct ccgggggaca
     7441 aaagccttgt catgcccctt tttcctctct aatgctcagg tcaaagcgct gaagtcatga
     7501 cgtcttgata aacggtgttc aagtagctga ccctcggcat tagttctgaa aaatgcaaaa
     7561 tatggaaagc tatttcccat ttttcctctc taatgctcag gtcaaagcgc tactttcacg
     7621 acctctatat tatcatggtt tattgactac cggtatccag atttctccca tatatttccc
     7681 gttggtaact cccatttcga ctacagcatt tggaccccca acataggcgt aatcttctac
     7741 ttctcttaaa acatgcccaa aggccgtatt cgtcaaatca tttcttaatt cttcaggcga
     7801 ttctgctaaa gcgcttacaa caagatacgt tccttttggg aatgggatga ttcgcgtagt
     7861 gtttggaaca tcctgactcg tttgaacacc gacatagtac atcattttcc cgttataggg
     7921 ttcgttgact gtaaagaaat aatgattttc agcagcttgt ttcaatgcat ctattgttcc
     7981 attaccgata actttttgct caaatgcttg tttctctgca gccaaaccga caaaatccgt
     8041 attgctactc ttcaattcca caccaatacc cgtcaccgta aaatctggtt tttcttcgat
     8101 cctatacttt gccatttctc ccaccctttc tattatatta agttcatctt acataaaatt
     8161 aaagggaaat gacacgatta cgctcttttt tctagggata tttttatttt ttaactattt
     8221 ttttgctttc actgcaatcc aaatctcact gtaaacatta ctgaaatcct ctccccattt
     8281 tgtaaaagag atctcgggag cttcgactaa cccataatct gaagaaggaa gccattctgc
     8341 atagattttc ccccaagtat cttgtaaagt ttggggaaat ggccctttat taggaaaaat
     8401 ggcccaagtg tgttcttcta tagcaagcat ttctaaatca gcgtacggat tttcttttgt
     8461 cgtagcaaag ccaatcatgt gcatcaaact gcccttttct tccaagcggt ctccatcaaa
     8521 tccataagaa acgttgatta cttgatttgg atataaatct cctaacttat gcatctcttc
     8581 cctttgagtc tccgtgatcg tctgcgctaa cttttgtatt tcttgattga ctccttcaaa
     8641 tttgacagga acttttttcg ccacacctgc cacataaaac tgttctttct tttcgatacg
     8701 aaattccatc gattttcctc ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt
     8761 ttgtatgccg tatctactga tttctgaagg cagatatcct gaccaatcac gaaaagctct
     8821 tgaaaaacct tcgactgttt catatccata tctaaacgcc gtttctgtca ctcttgatcc
     8881 atctagaaga tcgcgattag ccaaagccaa tctacggttt ttgatatagt ccattagcga
     8941 catccctgca ataaacgtaa agatgcgttt taattgatat tcagagattc ctgtattttc
     9001 ggctattatt ttataggaaa gtccttctgt caaatgttct tcgatgtaat cgatcaattg
     9061 gttcagatct ttaagcatat ttatcttcct ccttgattgc ctttttatca tattatcaaa
     9121 gaaagtcaat tacttgatga atcaggaacc aatttagtca gatgtagaaa atgttgtatg
     9181 gatctaaaga aaattctcaa gcagcgtttc gtaacggata attggtatta tctgcctatc
     9241 tatgttttac ctttagaaaa ataaattttg gggaagacag attctaagta aacaaagcgg
     9301 taaaaatatc ttttattccc ttagttaatc cgcttactac ctactgtaca aagcaaattg
     9361 ttgttcaaac tgctgatcag aacgctgctt ggatttttct ttgcgataca tcatacctct
     9421 tttatcaatt ggaacaatta tttatttcta acaaaatcac ttcatttttg tcagataaat
     9481 aaaaaacaaa aaaagttcca tctttaaaac acgctattct tttacttttt ctcacattct
     9541 ctaagaaaag aaaaggagaa tgaatttttg acaattcgag caaaagtgat tatcaaatat
     9601 gtaaaatcag ctactttttt cttaaacgtt tctttatact aaatataaat aagtgtttca
     9661 tgaaaggaga actagatgaa taattggctt gagtattttc ctgaaaacgt tttggaacgc
     9721 ggttacagct atcatctgca cggttttgtc cgtcatttga actatacaag caagtacttg
     9781 tccgcaacag tttctggcac ggaagattat aaggtagtga tcacttggga tgaaaagacc
     9841 aacatgactt gtgattgtct atatgccata gaaggaaaaa aatgcaaaca catggctgct
     9901 gtcttattcg cttacgaaga acgtccaata aaaaaatcca actactcact ttccgaactc
     9961 tcgtcccttg tttcctccgc tagttcctct cttgtacggg agcttttaac agagattctt
    10021 atagaacatc cgcactttat cgaacgcttc aaagttaaga tgccatttca tgcaataaat
    10081 tattcagata aattaacaac tatcatccat aaatacgatc atattatcaa gaaaaacaaa
    10141 aacaggaaaa cagcgaagtt catcatggag atgcgaaagt tcatccaaga agcagttgaa
    10201 tcgcttattc agcaaaatgc ctatcttccc gcattcgaat tgatcaatga agtgattgct
    10261 actctcgaaa cattctattg ggagcctgag gatgaacgaa cccttcttct gatagaggac
    10321 tgttattatt tatggaaaga attattagca gaagcacctc atgcagaaaa aaggcaaatg
    10381 ttctcttggt tcgtctgcca agtcgatcat actgacgcct cttattcaaa acgctactct
    10441 ataaaaatcc tgaaagaaga ctttcgggaa aaagaatttt ctaaccaaaa gaaaaaaata
    10501 gataaaaaac taaagaaacg gtaaagaaag acgatttcaa tgaaaaatag caagtaaacg
    10561 ataacaacca tcctaaatcc acaagaaata cagcagacct tgctttaaaa acacgaatca
    10621 tgcaaaggat gaagtaaaga agacttaacg cgtacccaat attagtcatt tgactattag
    10681 gtacgcgttt tgttttataa gtcatgacaa atttccttcg ttgctggata gattttccgg
    10741 cattgttgat agattttatg atacttttct acattttgta agagaggttc cgctgcatct
    10801 gtatattgga taaaattctg acaacaactt tcaggtgtgt cgaaccaccc taaccctagt
    10861 gcagcaatca ttgttgcgcc taatgcaggt ccttcctcca tacttaaaca atagaccgtt
    10921 gcatcgaaaa tatcagcttg gatttgtagc catgcaagat ttttcgcgcc tcctcccacc
    10981 gaaacaatac gtgtaaatct gcgacccgct agttctttac gtttcaatcc caccattgac
    11041 aaatgctcat taaaaattta gaatattcac aagtttaacg acaattatga aaatttatgt
    11101 cgtgctaaag gagttttgtt attggatgaa aagttagata taaaaactgc gatcaaagac
    11161 acgttaccta ccgttttcgg ttatatcggt attggacttg catttggtat cgttgggaaa
    11221 gctgccggat ttcatccact agtcgtcacg ttgatgtccc tgctggtcta tgctggttct
    11281 gcccaattta tcacagtcag catgcttgct agtcacagcc cattgctttc catcgttttc
    11341 tcgacctttc tagtcaattc ccgaatgatt ctgatgagta tgacgattgc tccttatttc
    11401 aagaaaaatc ggctgcttca aaatctgctg attggcacat tgctgacaga tgaaagtttc
    11461 gcactgggaa tgaataaatt gaactatact ggacaaaagt taaactttcg ttggatgaat
    11521 acagctaatt ggatttctta tttgacgtgg gtgggttctt ctcttgttgg cgcactttta
    11581 gggaatttta ttactgatcc gaaaaagttt ggtttggatt tcgccatcgt cgctatgttt
    11641 atcgggttac tttatcttca agtcatttct gatagaaaca cctctaaacg attacagttg
    11701 atcctgattg gtctcacttt gattctggta tatgtcggac tgatttttat cccaagcaac
    11761 ttagtgattg tagttgtaac actgattggg tgcggattgg gggtgtggat caaacatgcc
    11821 ttcttttgag tttacattgc tgactatttt aggttgtact ttagcaactt ggctttctag
    11881 ggtcctacca tttgtcttac tgaaaaaatt cgatttaccg caaccgttgc ttgagtattt
    11941 aagttttgtc ccgatcgtca ttatgtctgc tttatggttc agcagtctat ttacccaaaa
    12001 tatcggacat ctgccgcaga tcaatctcga aaatgctttg gcttcagttc ctacactttt
    12061 agctgctatt ctttcaaaaa gcttattggt gatcgtcttg gcaggaattc tttccctttc
    12121 gttgattcgc ttattataaa taaaaaaaca agagcttatg gcaaattact gtcatgcgct
    12181 cttgttttta tcaaagtcaa ataagaaaaa aatcggcgta ttcttgctta tccgttgaac
    12241 ggaccactaa caacgacttt tcctaacata tgtcctgttt ctacgtcttt ggttgcttgt
    12301 ttcaagttct tcgcattcat cccatctgaa tagaccttgc tcaatgtact gcggataatc
    12361 ccttcatttg ctaaagcagc aatgtgcgct aaagcttctc cttgcgtttc gatttcatag
    12421 ttatagtctg ttttagcgaa catatactcc cagtcgaagc tgatagattg gtttttccaa
    12481 acacctatat ctaacggctc tttgatccct acgatcgttc ccacatgtcc aaatggtcgg
    12541 acaaggtgct tgatttgatc aaagtagcgg gtaatatcga ataatacagc gacatactct
    12601 acttgatcga ttcctaattc acgaaaactt ttacttaagt cgtgatgata atcgattgga
    12661 tagtctacac ctgttttttt cagccattca aaattcttcg gacttgcagt cgcgtagact
    12721 tctaggccag cccaatgcgc tagttgattc aaaatcgaac cgacgccccc ggatccgttg
    12781 atcactaaga tttttttccc gcgattggca ttttcttctg gagttagacc aaatttttca
    12841 aataacaatt cataagcggt caatgaggtc aacggcaagg ctgcagcttc ttcatctgaa
    12901 agattttttg gtgcaagagc gacaatacgt tcgtcaacta gttggtattc ttgattgctt
    12961 cctgctcttg ttgttgtacc tgcgtaaaaa acgcgatcgc cgacagaaaa tttttgcact
    13021 tcgtcaccta cagcaacaac tttcccgact ccatcaaaac ctaagatacg taaagcattt
    13081 cttatcccgt tatcttggcg aagtttcgta tcgacaggat taacagatac tgcgttgact
    13141 tttaccagaa gatcccgtga actgggtaca ggtatctgtt ctttttcatc aataaagctt
    13201 tttggatcat cgatcggcaa cccttcataa aaaccgacta ccttcattaa ttccttttct
    13261 gacatacgaa ttcctcctta gcttcatcaa gttggagcat ccatacagtg ttcatttcaa
    13321 tatatatagt atgctatttg ttccaccttt tttattcatc ataattattc tcttacttaa
    13381 agtaaactat ttgcactttt ttggttaaaa aagaggttgc gacaagactt ttgtcacaac
    13441 ctcttataaa cggtgttctc ctaccaactc ttcggtcaca agtcaaaata ttcatcaaac
    13501 atgagaagct gttttttgaa attgctcctt gtgactcaga gttaaacggc aggctcacaa
    13561 cctctgatat aaacggtgtt caatcaactg accctcggta ttagctcaaa aaacgacaaa
    13621 atatgaagag ctatttttgt ttggctgttc ttattacttg atacaggaca gctttttcac
    13681 gacctctaat tcacggtgtt caagcaagaa atcattgttc actgaaaagc gttggatgtt
    13741 ctttttcaaa ggtctctaag atctctcgta cttcatcagt agatttcgta ttcatcaact
    13801 gaactcgcaa gtcatttgct cctggaaatc ctttgatata gattttaaag aaacgatgta
    13861 gtcctgtgat ggaacgcggg atgatttctg aataatgatc ttgcagatct agttgcagac
    13921 gaagtagtcc gatcagttct ttttctgtat gttccctcgg gtctttttca aatgcataag
    13981 gattcttgaa aatccctcgg ccaatcatga tgccatcaac gccatatttc tcggccaatt
    14041 cttctcctgt ctggcgatcg gggatatctc cattgatcgt gattagtgtt tgtggagcga
    14101 tttggtcacg taaagcaatg attttaggaa tgacttccca atgtgcatct actttactca
    14161 tctctttacg cgtacgcaaa tggacagaaa gattggctat atcttgatga agtaggtgac
    14221 tgatccattc ttccatctca gacatttccg tatagccgat acgcgtcttg acgctgacag
    14281 gcaaaccgcc agctttagct gcttcaatca attgtgccgc tacttctgga cgaaggatca
    14341 agccactgcc ttttccacgt tctgctacat tagggactgg gcagcccatg ttgatatcga
    14401 ttccttggaa gcccatttct gccatagcaa tgctcatttc tctaaaaaat tctggcttgt
    14461 ctccccaaat atgggcaacc atcggctgtt catcttctgt aaatgttaat cttcctcgta
    14521 cactgtcttt tccatctgga tgacagaaac tgtctgagtt tgtaaactca gtaaagaaga
    14581 catccggtgc tgctgcatgc tttacgacat ggcgaaatac aacatctgtt acatcttcca
    14641 ttggtgctaa aacaaaaaaa ggctttggca gctctttcca aaaattagta gtcatttatt
    14701 tgttactctc cattctatca agcagtgaag tcgcaatctt tctcaaaaac attgtcatta
    14761 tactaggaaa caaaaaaaga agcaaagtga acagcttaaa agatgtttct gattgagcaa
    14821 aaaaaagact gtgacaaaaa tcttatcaca gtccattttc cggaataaat ggtgttcaaa
    14881 agcttgcatt tgcggtaata agtccaacca aacgaaaaat aggaggagct atttttgttt
    14941 ggctgttctt attacttgat gcagaacagc tttttcacaa cctctccact aaacggtgtt
    15001 caatcagctg accctcggta ttcgctcaaa aaacaacaaa atatgaggaa ctattttccc
    15061 gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctctttt ctaacttctc
    15121 aatcctcttc ctttattgat caaataccaa gtaacggcgt acaaaacaac gatgaataac
    15181 acaagtacga tcatcgatgt tgctactggc acatcgatcg ttccaaggaa tccgtaacgg
    15241 aaaccggaga tcatataaac gatcggattg acttttgaaa cagcttgcca aaagggcggt
    15301 aacatagaga tggcatagaa aacgccacct agataagtta acggctgcaa gacaaatgta
    15361 ggcacgatcg atacatcatc ataagactga gcaaaaatcc cgttgagcag tcctgctaat
    15421 gaaaacaaga ttgccgtcat caataatgta atgatgacca tgaaccatga atacacatga
    15481 agtggtacaa agaacaagga aatgatcgtt acgagagagc cgacgagaat actgcgaccc
    15541 acaccaccaa tcacaaagcc ccaaataatc acatgcgtag gaacaggtgc taccaagatt
    15601 tcttcgatat ttttctgaaa tttttgagag aagaaggacg aagaaacatt ggcatacgaa
    15661 ctagtgatag cagacatcat aatcaaacca ggaacgatga attccatata tgaaaaacca
    15721 cccatgtcac cgatccgccc accgatcatt tttccgaaaa taacaaaata caaggatgtc
    15781 gtgatcactg gcggaaccag tgtttggacc catatacgca aatagcggtt cgtttccttg
    15841 atagctaaac tttttaatgc agtaaaataa agattaaaca tttttctcct ccgtatgatt
    15901 attttcttct gtgattttca agaacagttc ttctaaacga ttcgacttat tcctcataga
    15961 aagaactttg attccttgtc ttgtaagctg atcgaagatt ccgttcaccc cttgatttcg
    16021 ttcaacttcg acagcgattg tctgctcatc ttcaaatgta caagcatatc cttcaatcac
    16081 aggtttggta tcatatggcg ctaaatcaaa gataaatgtt tcaaattgca gttttgacag
    16141 aagctctttc atacttgtat tctcgatcag ttcacccgat tggatgattc cgatgttgcg
    16201 acaaagcatt tcagcttctt ctagataatg cgtcgtcaaa atgatcgttg taccattttc
    16261 gttcaattct cgtagaaact cccacatctc gcgtctcaac tcgatgtcaa ctcctgctgt
    16321 tggttcatcc aagatcagca aacgtggttc atgcatcaat gccctagcaa tcatcagtct
    16381 tcgcttcatt cccccggaaa gcatacgtgc acggacattt ctcttttccc aaagattgga
    16441 ctgcttcaaa tatttttcgc tgcgtttcat cgcttctttg cgagagacac cgtagtagcc
    16501 tgcttgattc acgacgattt gctggaccgt ttcaaaaggg ttaaaattga actcttgtgg
    16561 aaccaatcca atctgttgct ttgctcgaac tagatccttg tccagatcat aaccgaagac
    16621 attcactttt ccagatgttt tattgactaa agaagtaata atcccgatcg ttgtagactt
    16681 cccagctcca tttggcccta atagcgcata gaaatctcct tctttcaccg aaagatcgat
    16741 tccccgcaat gcttcgaccc cacttccata gatctttttc aaattattta tttcaagcgc
    16801 atttgccatt tatttcctat ctccttatag acatgattct taattgtatc atggaataca
    16861 gcagatgaaa aaacaacttg gcttccacac aaaaacacac ctgctaacaa agaggagatg
    16921 tgttttctag ttcatgatca tcttcgatag aataactatc taaacaactc tggaactttt
    16981 aaaatcataa aagtaccgac agcaactact acgatgacat atagaagatc cttataactg
    17041 aggttcttgt cttttttatt catgttaagc tttcctccgt ccttttatcg aaagcttttt
    17101 tcttttattt taaccgaaat aaataaaatg gaaatagtta ttttcaattt ctgttttcgc
    17161 tgtttgtttt acggagaact ttgccatgga acgtgcgtct cgtcgctgat gctaagtttt
    17221 ttcgataaaa gctgctagaa ggaagacagc gtggtttatt ttgtatcgtc ttttgctaaa
    17281 tcttccaaga tcatctggac agcagactcg ttcccattga attctgccac ttttttggca
    17341 tcaatgtact tcttttgata atcgtctttt tggacccacg catcatgctt cgtttgataa
    17401 agttaatcaa acggatacca ctgacgctgt tcatcgaaca acaaactttc tttttgggcc
    17461 tgttcaacta tggatttagg ataattcaaa actttcagca aggcaatcgc atttcgggtc
    17521 cttgaaggtc cttcttttaa tttgaaatca aaactgattc cttttccttc tgttacttgt
    17581 tcttcaaagt ggacattctc acaataattc tttaaaatct ctgtcaattc gatatcatgt
    17641 gtggcgacaa atgctaaaga tggatactcc gcaagccacc gaacgactga agcggaagaa
    17701 gcaatccgtt cgatcgtatt tgttcctttt aaaatctcat ctacgaagca gtagcagcgt
    17761 tctttggaag ccacttggtc cagcaaacgt ttgatcgatt tgatttctga aacaaaataa
    17821 ctgtcgcctt caaataaatc atcttcgatt gccatggaag tcaacacatg tccaggttgc
    17881 agggtaaact gttctgcaat cgctgtttgg atcgtctgtg ctagaataca gctgatggct
    17941 atacttttga catacgtaga ttttcctgat gcattggaac ccgtgactaa tgtattcttc
    18001 tgccagttga ctggattaac taccgcttct tttaacaatg gatgatagat atctttcgct
    18061 gtcacaccgc cctcagtaaa ctctggtcgg caagtgatcg gcatataggt gcgataattc
    18121 aaaacggctg ctgcaacttc caattctccc agcaattccc acagcttgat ggcttcagtt
    18181 gaatgctttt ctaatttagt gagaacgaaa tgataggaga taaatggcag catgaacatg
    18241 atgttgacat attcaaaaat caattctgct tcactcccgc tcttcattcg aaatgaaaaa
    18301 cctaatttag ggatgtctct taatggttta gtttgctgtt tcagctcttt ttgaagtggt
    18361 gtcgtcattt ttgctaactg attggcggct gaaatcgttg aaaccaaata actcatgcta
    18421 ttcagctctg tttctagtgt ttgtttcttc actagataat agaccgtatt gaatactaag
    18481 ccaccgattg ctagatagat tcctagtact tgccctaaaa gaagcaaacc tacacccacc
    18541 agcggaagca agccaagaaa aataaataaa cctacgcgtc caatcttttt actttttcca
    18601 tccgctagat attgttttgc aaaattatga tctttttttc ctaaacgagc gaattgatat
    18661 tggattcttt ctctcagttg aggattttcc tgataaaaag caattagttt ttctaactga
    18721 taatcttctc caaaatcaaa gctacgtaaa cgctgataca acgcttctga acctacacta
    18781 gaataggtac tgttgatccc ttcaaatact tcgaacatat ccaagtcata ccaagtaaga
    18841 tcgtcgattt cactatccca tgaacggaat tttttctccg tgagccacgc atccttcaaa
    18901 ctttcttctt tatcaaatct cggttgataa ggaatttttc cccaattact acgaactgtt
    18961 tcctttactt tcaaacgtga aaaaatgtct aaaacaacta ataaaataac aactgcaata
    19021 ataattaaaa cgataaattc tctttggccc atatccctct ccgttccttg atttgttagt
    19081 tttagtatag cactgttgcg ctatttgaaa agggggtaca aaaataactg tcttcgttac
    19141 ctaattcgta tcaatcacac caaaataaaa acaccttcca aaaaagaaga tgtctttaaa
    19201 acgagataca attttttgtc attcacttca ggtaaattag agccaattaa accctccgca
    19261 acttgacgga gatggagaag atgcccccgt ctaatctagc ggtcatctct cctcccattc
    19321 cttccaccaa tttttttgac aggtaaagcc cgattccgct agattccgta tttgtgcggc
    19381 tggtattttc ggtataaaaa cgctggaaga ctttttcaac agaggtttgc gctcctggtt
    19441 tgataggatt ggccactgtt aatcgaatga atgaatcctc ttttgaaaat gtcacctttg
    19501 cttgtccatc tgcataacgc aataaattct gtatgatatt ttgcagcact cgcagcaaca
    19561 tgagcggatc tgctaataca gttgtattta tcgagtgttc agcgaacgct acctcgatat
    19621 ttttctcaga aaattgttca taaaaagaga aaaatacttc ttccacctct ttagaaaaag
    19681 aaagcgtatg gagatccatt tctttttgct tcgtctctat tcgagcaaga tcataaaact
    19741 gatccgtcat actgagtaat cttgcaacag attgtttgat gatcgttaaa tacttctccc
    19801 gtttctccgt atcctctacc tgttccagta attgtacata accgttaata gcagtcaaag
    19861 gagttctgag atcatgagaa aggccgacga tcgatagtct gatgttttct tccatttctt
    19921 gaaatgcttg tcctttttgt tcattttcat ccacccatgt attcagtgca tctactaggt
    19981 ccattagatt cttttctcga aattcgactg aaagacgacc accatattct tgtcgttcag
    20041 aaagagtggc gattttctta gttaatctct tgatttccct ttgcaggaag aaaataaaac
    20101 ttcccataaa gataacaacg agaagcaaca gccacttaat caaatctaaa ccccactccc
    20161 cacaccgtat gaatatacac agcatgtgcc tttgctagtt tccctcgaag attggaaata
    20221 tgcatattca ctgttttctc actatcaaaa tatggttctt gccaaactgt ttcataaaga
    20281 tttgctcgcg aaaatacttt tttcggatgt tctaaaaaaa gcttcagcag atcgaattct
    20341 cttcctgtca aatggacagc cacaccattc acatttacat ccctcgactc tttgtccagt
    20401 aagatttcct tgtaatagat gcgttgcgac agatctttta cttggacaga agtctgatga
    20461 cgaagttgga tgccgattcg taacaacagt tcttcgatat caaatggttt cgtcacatag
    20521 tcatccgctc ctgctgccaa caaggaaact ttatctcttt gatcgctttt tgctgaaaga
    20581 acgatgatcg gaacattcga tgtctgtcgg atttctgcta acaatgcttc acctgaaagc
    20641 cctggaagca tgagatctag aaggatcaaa tcgacaggat atgtgcttaa caataatttt
    20701 ccttccgtac ctgaataagc atcaagaagt ttgtatctct gttcaagaac ctcctccagt
    20761 aaagaatgaa cagcttcatc gtcttcgata atcaaaatag tttccagcag gaacacctac
    20821 ttaatgtcag atttttggaa aataaacatt cctagagctg atgataccac gattgttact
    20881 agactgacca gaatcatttt cggtgcttgc gaataagttg cttgttgcgc aaagtccgta
    20941 tacatgagcc acgtttccgt aaaccactcc gttagaaatt ccaagccttt gatttttgcg
    21001 aaaacaccgg gaacgaaggc cgttcccatg atataaagaa aagaaagaag caaactgaca
    21061 ctggtacttc tagtgatgaa tgtcaaagcc actggaacag ataatgtcgc taatatcagc
    21121 agaagcaata caggaaatac ttttagaaca taatttccat agtttttgat tccctcaact
    21181 tcaccaaatc cgaaagcaag tgtgtaagca atcatggcta gccccgtaat caaaaccacg
    21241 ccaagtgttg tgatcaaaag cgtaacgaga agtttcgaca cataataagt cattcgattc
    21301 cgtcctaaag ataacgtatt gcgaatcgta ccgttttgaa attcttcccc aatataaaag
    21361 cacgcaaatg ctgcgaaagc cagaagaatc catgcagcca ttttttccac cattaaaatc
    21421 cctgttgcac cgttgatgac tgtatccgtt gcttggttta attcattgcc gtattttgta
    21481 tgcatatacc attcgttcca agaataaaaa agcggaagta cgaccatcag tcctagtgag
    21541 acccacattt tacggctctt ccatactttt attttttcag cagttaatag attaaacatg
    21601 tggattgcct ccagttaatt gtaagaaata atcttccagt ttttgcccaa ctaggtggat
    21661 accatccaca ggaacttttg cctctaccaa tgcttgattg atggctgcta cttggtcaat
    21721 tccttcaaaa agctggataa cacggctaga ttggacaaaa taatccctat aaccaagaga
    21781 gtcgagaacc gttactgctt gttccgtcgc cgaagtgtca atttttataa attgacgaga
    21841 ttcttgtgcc aattcttctt tcgataattc tttgatcaag tgtccttcat gcaaaatacc
    21901 gtagtgagtg gcgatctgtg acaattcatc tagcaagtgg ctagaaagca agacggtgat
    21961 gccccgttct tttaccaaac gttggatgat ttctcgcatt tctgccatcc ctgctggatc
    22021 catcccattc gtcggttcat ccaaaatcaa aaattctgga ttcgtgatca gtgcatttgc
    22081 aatagctaaa cgctggcgca tacctaaaga gaaattcttt gctttctttt tccctgtatt
    22141 ttccaatcgc atcattttca gcaggtcttc gatttctcta tccgacacac cgccgttcgc
    22201 tgcttggata cgtaggttat ctcttgcagt taattctgga taaagagcag gggtttcaat
    22261 cgattgaccc atacgtcgtc ttgctgcctg caaatccgtc gttccaaata attcgatcga
    22321 accttcatca atcgagatca aacccataat agttctcata aaggtagact tacccgctcc
    22381 attctcccca atcaaaccat agatcattcc acgtttgatt tcgatcgata ctttatctaa
    22441 tgctttggca gcaccgtatt ttttggttat accagttgct ttcaagaccg tttcatttcc
    22501 aaaatccata cttatccttc tttcttattg ataagtgaag tatagttgac gggtataaag
    22561 aaagcagcca gaaatgtata aagaaagtgt aaattttcca attgaatttc aagaaaaatc
    22621 agactgtctc gcagacgtgg ttgagttgtc tgccaatcaa agagcgcacc aaacagtgct
    22681 aatccaagtg gactggccac catggtaatc gttgttatcg ttgacatcag acgtccttgt
    22741 atatgcgttg gaacttctcg ctgaagccag accatataaa aagcaaaaga aaacattaat
    22801 ccttaaaaac ggacttatga aaaataatac atgccgtttg ggatttacaa ttagctacca
    22861 aataagaggt tatgacaaaa gacttttcac aatcccttct tccgaataaa cggtgttctc
    22921 ctgccaactc ctcggtcaca agtcacaata tttatcaaac atgagaagca atttttgtaa
    22981 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcagaagc ggataaataa
    23041 tattgtttga tgatacttta tctttttccc gtttgtttat agatcgaatg atattataga
    23101 ccaagaatcc tacctattaa catagctaaa aacacaaaaa aggcactttc caaattgtgg
    23161 aaagtgccag aaaagttagg attaaggtaa ctgcgctcag cattgcttcg cccagtacga
    23221 ttctcgtaac tcgtgaactc gaacgataat cgcaattaac gttttgagaa ctgtgaagct
    23281 ttacgggctt tcttaagacc tggttaaaca aaactctatt ttattgcatt ttacaggaat
    23341 gtatttcatg cgaaattaat aagtctttgt ttatagagtt ttactagggt ataaaaagtt
    23401 gtatgttatc attacctcaa aacattacct cagcttggcg agttggttga ttgaagaata
    23461 aatcatttat ctagatatct caataaaaaa tgatttctaa gaaacttgta ttttcgtgtt
    23521 attatttttt tgccctatca aaatttctaa ttcttctagc gtaatatgat tatgacacat
    23581 aatcgattca attacttcaa tacctatttt tttcgagaaa gatccctttt gttcactcac
    23641 aaaaatactt tcagaagaca tatataagga acgaagtttt ggaattagta tatcgtacaa
    23701 atagaccacc ataaaatcgt ccttgcaacc attctcaaaa agaattaaat ttgctgattg
    23761 tgttacatat atcactgaat tacattcatg gtcaattcca aaaacaatgt ctacttcatt
    23821 ttcttttcta attcgcgcag ttacattttt caaactttca ccattactta taaatgcttt
    23881 agttactgta agctctgatg tttgttcaga tattcctttt ataatatttt gttcatcagc
    23941 cacattcccc ataaatccgc cgattccttt taagtaataa ttctcaccta agagtcgctt
    24001 gcattcactg aattgataaa aaaaccagtt aaaaaaatct tctggaaaaa taaattctga
    24061 tcgaacttta tttatatttt tagctcctat taattttata actcgattaa tgttcacttg
    24121 ctgcgatgaa catataactg aataaattga gttgtttaat tcaaaaaaac aactttaaca
    24181 tatgcatcat taattcgtta ttttttaggt agatattgat cattgttatc tctctttttt
    24241 tgcttcccat actccaactt agaaattgcc gttataaaat ttattttaaa ggtgactttt
    24301 tcccctgaag tatcggtagt aggaatactt aatacctcag cttctgagga ggtatcccaa
    24361 ttatagttat actttgcatc atcaccataa agaacaattc catttttttt atcttgtgcc
    24421 gttagcttta ctggttcgct tacttttctt gttaatatct tggactttac atcatcgata
    24481 ttgcttattt tatcaagctt aaataattta caaattaatt tcgtactagt ttttatattt
    24541 gacgagagtg taaaatattt tgtgtaaata gaaaaaagga agtcccttct gtagaataga
    24601 gttaccacaa cacattcaca gaaaagagga cttccatatg aacgatttta ctacagaaat
    24661 tctaaagact ctagcgaaca aaggcgattt gaatgaatta ttccgtgtcc atttggaaaa
    24721 agctgtcaat acgcttctca aaacggagtt aacggctttc ctcgattacg aaaagtacga
    24781 tcgcattggt tttaacacgg ggaattctcg taacggctcc tatgaccgta cggtcaagac
    24841 cgagtacggg gaacttcatc tccagattcc gcgcgaccgc aacggcgagt tcaagcaaca
    24901 gactgttcct gcttatagac ggacgaatga taccttagag gagaccgtca ttcacctctt
    24961 ccgaaaaggt attaccatgt cggaaatcgc agacttgatt gagaaaatgt atgggcatca
    25021 ctacacgccc caaaccatgt ccaacatgac taaagtgctg actgaagaag taaatgcatt
    25081 taaagttaga actctaaatg ataagtatgt cgctattttc atggatgcta cttatattcc
    25141 tttaaaacgg caaaccgtct ccaaagaagc aatctatatt gccattggta tacgagaaga
    25201 cggcactaaa gaagtactaa gttatgcgat tgctccgact gaatcgacct acgtttggaa
    25261 tgagttgtta caggacattt actccagagg ggttcatgat gtcttactgt ttattaccga
    25321 tggcttaaaa ggcatgaaag atacgattca ccagatttat cctaaagcta aataccaaca
    25381 ttgctgtgtt cacgtttccc gtaatattgc tcataaagta cgtgtcaaag atcgaaaaga
    25441 aatctgtgat gattttaagg ctgtttatca agctagctca aaggaagagg caaatacctt
    25501 tttagggagt atgattgaga agtagcaaaa aacttatcct aaagtgacgc agtcactgat
    25561 aaaaaatcaa gatttattga ctttttatga gtttccgcct ggtatccgcc gaagcattta
    25621 ctcaactaat ttaatcgaat ctttcaagaa acaaatcaag aaatacagcc gcagaaaaga
    25681 gcagtttcaa aatgaagaat cactagagcg tttcctagta tccatctttg atacatacaa
    25741 tcaaaaattc ttaaatagaa gccataaagg cttccaacaa gtgacggata cattagcttc
    25801 aatgtttact gagtaaccca ttattttgca gaaggacgag ttatttacac aaaattattg
    25861 acgctccctc caaattactt catcaaaaca ttgatataat ttttattata tcagtattta
    25921 ttcttgatac ataaatgaga aaataaatac tttaaatcta ttgactacaa gtagtcatta
    25981 agtcttcata agatgtacaa tatgacatta aaaaaataaa agcttacgac catccgttaa
    26041 atctgacact aaataatgga ctgctttctt ttgtattagc aggttacgtt gactatgaat
    26101 attcaccaga ttatttggag aaacctcttc aacacgagaa caatgatatt aattttagat
    26161 ttgtctacga gtcaaaaagt aaaacatggt tagttgatca attaagcacg ataaacacaa
    26221 gcttccatga attacctaaa cctgaaaatg aagttgagat ggtcattaaa atcttctaaa
    26281 aaagaagcgt tgaaggggaa atattccttt caacgctctt ttttatatgc ttagaaacat
    26341 aaataaattg tatactttct ttttagcccc tattactttt cacgaaaaat atcttttttt
    26401 cgacgctagc gtttctctac ttctaatact gtacacagtt tttgtattat tgaaaaatta
    26461 ctcattattt agcattaatc cacatcgttt tatcaatcga ccttcatact aaactacttt
    26521 aaagtcatct tccttctcgt ttttcatta
//
LOCUS       DAZUMM010000033        26058 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000033 DAZUMM010000000
VERSION     DAZUMM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.033"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG8E6_001331"
     CDS             212..604
                     /locus_tag="ACG8E6_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5061756.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG8E6_001332"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG8E6_001332"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFS5061757.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG8E6_001333"
     CDS             2698..2943
                     /locus_tag="ACG8E6_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061758.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3164..4774
                     /locus_tag="ACG8E6_001334"
     CDS             3164..4774
                     /locus_tag="ACG8E6_001334"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFS5061759.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4751..4963)
                     /locus_tag="ACG8E6_001335"
     CDS             complement(4751..4963)
                     /locus_tag="ACG8E6_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061760.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5066..5935
                     /locus_tag="ACG8E6_001336"
     CDS             5066..5935
                     /locus_tag="ACG8E6_001336"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFS5061761.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6100..7371
                     /locus_tag="ACG8E6_001337"
     CDS             6100..7371
                     /locus_tag="ACG8E6_001337"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS5061762.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7368..8663
                     /gene="rho"
                     /locus_tag="ACG8E6_001338"
     CDS             7368..8663
                     /gene="rho"
                     /locus_tag="ACG8E6_001338"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFS5061763.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8762..9025
                     /locus_tag="ACG8E6_001339"
     CDS             8762..9025
                     /locus_tag="ACG8E6_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFS5061764.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9470..9919
                     /locus_tag="ACG8E6_001340"
     CDS             9470..9919
                     /locus_tag="ACG8E6_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5061765.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9976..11394
                     /locus_tag="ACG8E6_001341"
     CDS             9976..11394
                     /locus_tag="ACG8E6_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS5061766.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSISTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11394..12254
                     /locus_tag="ACG8E6_001342"
     CDS             11394..12254
                     /locus_tag="ACG8E6_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS5061767.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12428..13201
                     /locus_tag="ACG8E6_001343"
     CDS             12428..13201
                     /locus_tag="ACG8E6_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5061768.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13348..13713
                     /locus_tag="ACG8E6_001344"
     CDS             13348..13713
                     /locus_tag="ACG8E6_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFS5061769.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13861..15069)
                     /locus_tag="ACG8E6_001345"
     CDS             complement(13861..15069)
                     /locus_tag="ACG8E6_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5061770.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15088..15366)
                     /locus_tag="ACG8E6_001346"
     CDS             complement(15088..15366)
                     /locus_tag="ACG8E6_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061771.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15559..16215
                     /locus_tag="ACG8E6_001347"
     CDS             15559..16215
                     /locus_tag="ACG8E6_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5061772.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16657..17454)
                     /locus_tag="ACG8E6_001348"
     CDS             complement(16657..17454)
                     /locus_tag="ACG8E6_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFS5061773.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17451..18635)
                     /locus_tag="ACG8E6_001349"
     CDS             complement(17451..18635)
                     /locus_tag="ACG8E6_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFS5061774.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18660..19907)
                     /locus_tag="ACG8E6_001350"
     CDS             complement(18660..19907)
                     /locus_tag="ACG8E6_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS5061775.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVCPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20122..21867
                     /gene="dnaX"
                     /locus_tag="ACG8E6_001351"
     CDS             20122..21867
                     /gene="dnaX"
                     /locus_tag="ACG8E6_001351"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFS5061776.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21887..22201
                     /locus_tag="ACG8E6_001352"
     CDS             21887..22201
                     /locus_tag="ACG8E6_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFS5061777.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22226..22822
                     /gene="recR"
                     /locus_tag="ACG8E6_001353"
     CDS             22226..22822
                     /gene="recR"
                     /locus_tag="ACG8E6_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFS5061778.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22886..23530
                     /gene="tmk"
                     /locus_tag="ACG8E6_001354"
     CDS             22886..23530
                     /gene="tmk"
                     /locus_tag="ACG8E6_001354"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS5061779.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23545..23874
                     /locus_tag="ACG8E6_001355"
     CDS             23545..23874
                     /locus_tag="ACG8E6_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFS5061780.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23888..24826
                     /gene="holB"
                     /locus_tag="ACG8E6_001356"
     CDS             23888..24826
                     /gene="holB"
                     /locus_tag="ACG8E6_001356"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFS5061781.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24862..25686
                     /locus_tag="ACG8E6_001357"
     CDS             24862..25686
                     /locus_tag="ACG8E6_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFS5061782.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25679..26026
                     /locus_tag="ACG8E6_001358"
     CDS             25679..26026
                     /locus_tag="ACG8E6_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFS5061783.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
BASE COUNT         8487 a         4628 c         5556 g         7387 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga atttcggctc cctattctta ttgaagaata gggagctttg tttattgtct
     3121 gagacttttc ctgcacataa attaaaaagg agtttttgaa cttatgacaa aatatatttt
     3181 tgtgaccggc ggtgtcgttt catcaatcgg aaaagggatt gttgcagctt ctttaggacg
     3241 cttattgaaa aaccgcggct taaaagtaac tatccaaaaa tttgatccat acatcaacgt
     3301 ggatccgggg acgatgagcc catatcagca cggagaagta ttcgtaacag atgatggtgc
     3361 tgaaacagat ttggatctgg gccactacga acgttttatt gacatcaatt tgaacaaata
     3421 ctccaacgtg acgacaggaa aaatctattc tgaagttttg cgtaaagaac gtaagggcga
     3481 atacttggga gcaactgttc aagttattcc tcatattaca aatgagatca aagaaaaaat
     3541 catgcgagca gctaaaatga ctgattcaga tgtgatcatt acagaagtag ggggaacagt
     3601 aggggatata gaatctttgc cattccttga agcacttcgt caaatgaaag cagatgtcgg
     3661 tacagataat gttatgtata tccatacaac attgattcct tacttgaaag ctgctggaga
     3721 aatgaaaact aaaccaacgc agcacagtgt aaaagagcta cgtggtttag gtatccaacc
     3781 gaatattttg gtggtacgta cagagcaacc tgtttctcaa agcgtgaaaa acaaattggc
     3841 acaattttgt gatgtcgaac cagaagcagt tatcgagtcg ccagatgtag atacacttta
     3901 ctccatccca ttagttttac aagcacaagg aatggatcaa attgtttgcg accatttaaa
     3961 actagataca ccagaagcag acatgacaga atggaaagaa ttagaagaac gtgtcttgaa
     4021 cttgaagaaa aaagtacgga ttgcgcttgt cggtaaatat gtcgagttac cagatgcata
     4081 tatctcagta gttgaagcat tgaaacattc tggtttcgcc tataattcag acatcgagat
     4141 cgattggatc aaagcacaag aattaacgag agaaaatgta gaagaacgtt tgaaagacgc
     4201 agatggaatc ttggttcctg gtggatttgg tgatcgcgga gtagaaggga aaatcgaagc
     4261 gattcgttat gctcgcgaaa atgacgttcc gtttttaggt atttgtcttg gtatgcagat
     4321 ggcttgtgtc gaatttgcac gtaacgtcgt tggattagaa gatgcgtcat cagctgaaac
     4381 gaatccagat acagcaaaca atattattga tttaatggct gatcaggaaa acatcgagaa
     4441 tttgggtgga actttacgcc ttggattata cccatgtaaa atcaaaaaag gaacgaagac
     4501 ggctgaagca tatgatggag ccgatgttgt gcaagaacgt caccgtcacc gctatgagtt
     4561 caataacaaa tatcgtcaat tgtttgaaga acaagggcta gtcttttcag gtgtctcacc
     4621 agataaccgt ttagtagaaa tcgtagagct gccagagaaa aaattctttg tgggatgcca
     4681 attccaccca gaattgattt ctcgtccaaa tcgcccgcaa aaattaatca aagcatttgt
     4741 cggagcatca ttagcagatc acgaaacaaa ataatttttg aacttgatag aaagagaaaa
     4801 gtagcaagag aaggggataa gcaattttct tgtcactttt ctctttttct gatttgttat
     4861 tatttgtagg aaaaaagata gaaagcaaaa gacttttttc gattaaagcg ctattggata
     4921 ttttattttt tgcaaaaaac ttcttgaaaa ttttcatttt cattgatttt agtagaaaac
     4981 taccgaaatg tttggtaaaa tagggtcgtt ggttaagaat tgtcttaaca aatttagttt
     5041 tacaaaactt aggaggaatt ttattatgcc agtagtatca ggagctgaat ttttaaaagc
     5101 tgctcgtaaa ggcggttacg ctgtcggcgg attcaacaca aacaacttgg aatggactca
     5161 agcaatcctt gaagcggctg aagcaaaaca agcaccagta cttatccaaa catcaatggg
     5221 tgcagctaaa tacatgggtg gttacaaaat ctgtaaagat ttaatcactg atttagttga
     5281 agcaatgaac atcactgttc cagttgctat ccacttagac catggtgatt acgaagctgc
     5341 tttggaatgt atcgaattag gctatacttc agtaatgttc gatggttcac atctaccatt
     5401 tgaagaaaac ttgaaattag ctaaagacgt tgttgaaaaa gcacatgcaa aaggtatctc
     5461 agttgaatgt gaagttggtt caatcggcgg agaagaagac ggaatcatcg gtaacggcga
     5521 attagctgat attgaagaat gcaaacaaat ggttgctaca ggtatcgact acttagcttg
     5581 cggtatcggt aacatccatg gtcaatatcc agaaaactgg aaaggattag cttttgacca
     5641 cttacaagca attgctgatg ctgtaggtcc agacatgcct ttagtattac atggcggatc
     5701 aggtattcct caagatcaaa tcgaaaaagc tatctcaatg ggtatctcaa aagtaaatgt
     5761 taacactgaa ttccaattat catttgctgc agctactcgt aaatatatcg aagctggtaa
     5821 agataaagaa ggtaaaggat tcgaccctcg taaattatta gctccaggta aagcagcaat
     5881 cgttgaagat gctaaatctc atatcgactg gttcggttca gctaacaaag cgtaagcatc
     5941 cacataacag atacgatata taggaatgga gaacgacttt tgaagtcgct ctccattctt
     6001 ttttacttaa aacggcagac catgctcgtt tctttttttt atctatgata aaatacaagc
     6061 atatccatga aaaaagatta ataaacaagg tggaaacaga tgaaaaaaat cgtaatcaaa
     6121 ggaaaccgac ctttagctgg tgaagtaacg atcagcggtg caaaaaatag tgcagtcgca
     6181 ttgattccag ctgcaatctt agctgattcc ccagtgatct tggaaggtgt gccggacata
     6241 caagatgttc attcattaat tgaaatttta gagatcatgg gcgcaactgt ccatttttcc
     6301 aacaacatac tagaaatcga tcctcgagaa gtcgtatcga ttccgatgcc gcacggaaaa
     6361 atcaatagtt tgcgtgcttc ttattatttt atgggaacac tgcttggtaa atttggcgaa
     6421 gcggtagtag gcctgccagg tggctgttat ttaggcccta gaccgattga cctacacgtc
     6481 aaaggatttg aagctctagg tgctgaagtg acaaatgaac atggtgccat gtatttacgg
     6541 acagaaaata aaggattgcg tggcaatcgg attttcatgg acgtggtttc agtcggagca
     6601 acgatcaatg tgatgttagc tgctgtaaaa gcaaaaggac agacagtaat tgaaaacgct
     6661 gcacgagaac cggaaatcat agatgtggca actttactga ataatatggg ggcaaaagtt
     6721 cgaggagcgg gtacagatgt gatccgtatc gaaggagtcg aaacattaca tggctgtcgg
     6781 catttcatga ttcctgaccg catcgaagca ggaacctatc tagcattagc agctgcagta
     6841 ggaaatggta tcaaagtgaa aaatgtgatc tttgaacatt tggaaagctt tatagcaaaa
     6901 ttgcaggaaa ttggtgtcca tatgaaaatc agcgaagatg aaatcgaagt ttacccctcg
     6961 aaagaattaa aacccgcaaa catcatgacc tatccttatc caggatttgc cacagatctg
     7021 cagcagccac ttactgcgtt actattgatg actacaggca cttcagaaat catcgatacg
     7081 atctatgcga aacgtgtgaa ccatgtgccg gaactagcac gaatgggcgc tgatattacc
     7141 gtggaaggaa atatgatcat cgtgaatggt ccaaataaac ttcacggcac agaagtcgtg
     7201 gcttctgatc ttcgggcagg tgcttgttta gtgattgctg gactattggc agaagggaca
     7261 acgacgattt acaatgtaga ttatatctta cgcggttatg atcatattat cgagaaacta
     7321 acagccttag gggcatcaat tgaaatgata gaggaagtgg gagctgaatg agcgattatt
     7381 tgacaatggc agaactagag aacaaaacgt taaaagaagt ttatagttat gccaaagaat
     7441 ttaaaattcc ttattacagt aagatgaata aaaaggaatt atctctagct gtgattcgtg
     7501 cacaagcaga gaaacaagga ttttattata tggaaggaat attggatatc gttggtcaag
     7561 atggttacgg ttttttacgg ccaatcaatt atggaccaag tgcagaagat atctatattt
     7621 ctgcctcaca aatcagacga ttcagtttga gaaatgggga caaagtggca ggaaaagccc
     7681 gtccacctaa agaatcggaa cgttattatg gtttgatgca cgtggaaagc gttaatggaa
     7741 aagatccgga agaagcaaaa gaacgtccgc attttccagc cttaacacct ctttatccgc
     7801 aaagacagct tcaactggaa acaacaccgc aaagactatc tacccgaatg atcgatatct
     7861 tttctccaat cggttttggt cagcgtggat tgatcgtcgc accgccaaaa gcagggaaaa
     7921 cttctgtatt aaaagaaatc gcaaatggta tcaccgaaaa ctatccggaa gtagaattaa
     7981 tcgttctact gattgatgaa cgaccggaag aagtaacgga tctagaacga agcgtgaaag
     8041 gcgacgtggt atcttctaca tttgatctgc agccgcaaaa ccatactcga gtttctgaat
     8101 tagtattgga acgcgcaatg cgtttagtcg aagataaacg agatgtcgtt atcttgatgg
     8161 atagtattac tagacttgct cgggcctata acttagtcgt tccaccaagc ggacgtacat
     8221 tgagtggcgg gattgaccca gccgcattgt ataaacctaa gaaatttttc ggggcagcaa
     8281 gaaacatcga agaaggtggc agtttgacga ttttagcgac cgctttagtc gatacaggaa
     8341 gccgtatgga tgacgtgatc tatgaagaat tcaaaggaac tggaaatcta gaattacaac
     8401 tttcacgtga attatctgaa cgtcggatct tcccagccat cgatatcaaa aaatcaggta
     8461 cccgaaaaga agaattattg atgccaagtg aagaattaga agaaatttgg aaacttcgaa
     8521 aaaatatgac tggagattca ttagaatata cggaacaact gatccgcttt ttgcgtaaaa
     8581 caaaaaacaa tcagcaattt ttcaaagaat ttcaagatgt ctcctttggt aaaaaaaatc
     8641 cgaccagaaa tctgaaaaga taattgcaac cttgactgaa acatgttaag atgttaatgt
     8701 tgtaaatcga caaatcaact ctggttcagc aaatggagca gggcaaagga gcgaaaaaat
     8761 catgaaagaa aacatccatc cagattacca cccagtagta ttcatggatt caacaactgg
     8821 tttcaaattc ttgtcaggtt caacaaaaac ttcacaagaa acagttgaat gggaagacgg
     8881 taatacatac ccagtcatcc gtgtcgaagt aacttctgac tcacatccat tctacactgg
     8941 acgtcaaaaa ttcacacaag cagacggacg tgtggaccgt ttcaacaaaa aatacggtct
     9001 caaagacgca aacgctgcgg aataataaaa acgctgataa ctcaacgttt agcagactat
     9061 ctgataggga tagtctgttt ttttatgttt tttgccaatt taaaacttcg tttgatgctg
     9121 aaaaagtcgt aggatcataa tgcacaaaac aatgaaaaac tagtatatag ttttaattat
     9181 ccacttgata aaaacacctt atattaacga aatgaagagg ggttatttcg aagtaaaaaa
     9241 ccatttacct aaaattagaa gctaagcttt cctttcaagg aaggcttttt gttttgttat
     9301 agacttgcga ttgtttgtga gctagatcgt tcattttaat gacttataca cgcggatgat
     9361 gggtcttttt cctctgttca ttcataatat tgaacggtac agctagcgat gctatactga
     9421 cctcagttcc aagaaaacgt taacattttt tgtgggggag aggtttttta tgaggtttga
     9481 tcaattgatc gcagaagaac tgattgtttt cgatccatcg atagattcaa aaggatcatt
     9541 atttgagcga ataggggatg tgttgaaaaa aacaaataag gtaagaaatg ttcaaaaagt
     9601 cgttaaggca ctattcaaac gagaggagga gatctccaca ggaatagaag atggatttgg
     9661 gattccccat gcaaaaagca aatatgtgac aaaaccagca ctgtgttttt ttcatacagc
     9721 caaattaacg gactatagcg ggatagatga aatgccaatt gaatatattt ttgccatcgt
     9781 agttcctgaa aaatcagcag atatgcatct agagattctc agcacactct ccagacgatt
     9841 gatgaatgca gattttagaa agggaataaa agaagccact acgccagcag atatcatcag
     9901 acagattagt caagaatgaa aatagtagag tctttgtccg agtatcgata gtgaagaaaa
     9961 aggaggaaaa taatcatgaa aatcgttggt gtaactgctt gcccgactgg aattgctcat
    10021 acttatatgt cggcagaaaa attagaagta acagcaagaa atctaaacca tcaagcaaaa
    10081 ttcgaaacac aaggtgtaaa aacagaaaat cagttatctg aacaagaaat caaagaggca
    10141 gatgcaatca ttcttgcagt agataaagaa atcgaattgg accgatttgc cgggaaaaaa
    10201 gtaaagcgtg tctctacaag ccgagcaatc aaagaaccgc aagtagtaat agacgaagca
    10261 ttgagagata ttggcgtatt cgttgtgtct aatgaaaaag aaccagctgc aaatgagaaa
    10321 aagccgacaa tctataatca tttcatgaat ggtgtgaatt acatgcttcc atttgtgatc
    10381 gcaggcggaa tcatcatcgc actgagtttt gcctttggga ttacagcggc tgatcccaac
    10441 tcagcagact ataatgtgtt agcagctgca ttttcaagga tcggaggaga tacagcgttt
    10501 gccatgatgg ttcctgcgtt aggcgcgggg attgctacat cgattgctgg aaaagcaggt
    10561 tttgccccag gaattgttgc cggattactg gcctctacag gtggttcagg ttttcttggc
    10621 ggaatgattg gtggcgtgct agcagggtat atctgtgatt ttctggcaaa taaaattagc
    10681 gtaaaaaaag aattttctgc tatttatcag ctgattgtcg tccccttttt atcgatctta
    10741 attattgggc ttttgatggt atttgtgatt gaacaaccga tcacatgggt gttggatgct
    10801 ttgacaaatt ggctgaatag cttgggaaat acatccgggc tattgttcgg attacttatc
    10861 gggatcatga tggcagctga tatgggtggg ccgatcaata aatcgatttc gactttctcg
    10921 atcggtttga tgtcagctgg agtaaatgca ccgattgctg cttgtatggc tgcaggaatg
    10981 acacctcccc taggtattgc tttagccact ttattattca aacataaatt taccaaagaa
    11041 gaacggactt caggtcaatc atgctggatt ttaggcgctt cgtatattac agaaggtgct
    11101 ataccgtttg cggtatctga cccgatccgc gtgataccta gtttgatgac aggttcagca
    11161 gtcgctgcag ggatttccat ggcagtagga gttacttcaa tggcacctca tggagggatt
    11221 tgggtgatgc taattccaaa tgttatgaac aatttaccta tgtatgtctt ggcaattgca
    11281 tcaggtacag tcactacagc ctttatgctt ggactattga aaaaaacagt gaaagagaaa
    11341 aaacaagaca agtggacgaa aaatgaaaat ctaacaaatg aggtggaagc gtaatgttat
    11401 atacaatgaa agagttattg aaagtggcta aagagaatca atttgcagta ccagccttca
    11461 atatttgcag ctatgatatg atgaaagcaa tcatcgaaga agcagagaga ttagaagcac
    11521 cagtaatctt agaaattcat cctgatgaaa tcgcctattt aagtgatgag tttgttgcgg
    11581 cagtaagagc ctatgcttat acaagtaaac tgccgatcgt tatccacttg gatcatggcg
    11641 gtacgataga agatgtctta cgggcgattc gaaacggcta ttcttccgtg atgatcgatg
    11701 cgtctttgaa atcattcgac gacaatgtac agattacgaa aaaagtggtt gcgttagctc
    11761 atgcggtaaa tgtctcagtg gaggctgagc ttggaacgat tgggaataat ggttctgctg
    11821 aaggaggtgc tgaaacaatc atttatacag atcctgatca agcgaagatt tttgtgaaag
    11881 aaacaggaat cgatacactt gctgtagcaa ttggaacagc gcatggcttg tatccgaaaa
    11941 acagaactcc aaaattaaat atcgacttac tgaaagaatt gaaccaacaa atcgatcttc
    12001 catttgtttt acatggcggt tcaggtaatc cggacgcaga agtcagcgaa gcagtaaaat
    12061 acggagttgc caaagtgaat ttgagctcag atcttaaaag cgtctttttc aatgctttga
    12121 gagaaatatt gagtgaaaat ccatcgatgt acgagccgaa tgcaatttat ccagcagcta
    12181 ataaaaaggt acaagaagta gtcaaacata aaatggaaat tttgaacaca gtaggcaagg
    12241 ctagtttata ctaaaaataa aaatgtgtgg tcaagagggt atgacaatag tcttgtcata
    12301 ccctcttaac cacacacgat gttcagaagt agttttttcg aaaaatttct tcttatttct
    12361 cgaagccaat cacttctgtc acaacctctt ttttcctgtt actatataaa gtaaatgggg
    12421 tgattttatg ttgaatgaag tggagaatag acagcaggag attgtgaatt atctaaaaaa
    12481 caaccaattt gcaagagtca ccgatttgat cgagttagta aattatagcg aagccacgat
    12541 taaacgtgac cttgtttttc tggaaaaaag tggattgatc cgtagagttc gcggtggcgc
    12601 catgcttatt gacaatcgaa aaattgacgt gccttacctg atgaagatca ccaaattaga
    12661 agaagaaaca gaaaaacgta agattgcaga tacagcttct tctttgattc gagatgatat
    12721 ggtcttattc ttggattcca gtacaacctc tttacactta gtcagaaacc tagcaagatt
    12781 tgatggtttg cagatcatta cgaatggcat cgtcacagct gctatgttga gtgaatatac
    12841 aacggcaaaa gtatctgtat tgggaggggc aattgttccg aaacgagcaa caatcaacgg
    12901 ttcaaaagcc tataacgatg cattgtcata ttatgcggat ttagcgataa tgagttgtcg
    12961 cgggctagat ttcagccaag gagtaacaga aactcatgaa ggagaggcaa tggtcaaaaa
    13021 agcttttaga aaacaatcag accggcttgc cgttttagtt acacagcaaa aaattgcaca
    13081 taaattcatg catcaagcca ttgcttttca tgaaatcgat tatctggtga caagtaaaga
    13141 actgacaaaa gaacagtatg cgaaagtaaa ggaacaccat atcgaatgtc tgtacggata
    13201 aattattacg tgaatgttct aagtggcttc ctcattttta gataggacct tatctttgct
    13261 cgcttctctc agccgatggt ataataatga aaaggttatc ttgttagata atcttacttt
    13321 tttatccagg agaaaggagt gcagaaaatg aaacatgtga aggcgttggt agtgaaggca
    13381 atcatgatct gggcgattct gtgggtcgtg ctgacaggtt tatacggtgt tagctttatg
    13441 gattcaacga ttgttggggt aattattgta gtcatgatct atgtgctagg ggatctgttg
    13501 atcttacgta aagtagggaa catcgcagcg acgatcgcag atgctggttc agctgcagtt
    13561 atcttgtggc tctatctata ttttatgaat gatacagtcg atatttggat gaaagtattg
    13621 attcctgctc tattgatcgg tgtggcagaa tggttcttcc acaagtggtt gctaagtcaa
    13681 ggcatcgtac cagatgaacg gaaaatggaa taaatcaaca gttaaactat tcttatcgat
    13741 aaatgaaagc tttaaaaata aatagatgtt taattaagaa taatcataaa ctatttttac
    13801 tcaaaaaacc gctcagatta ttttcagatc tgaacggttt tttttatcta ttcatcaaga
    13861 tcaaatttca tttttcaatg attcaatcaa ttcgccctct gcttcatgac tatgttttcg
    13921 gccattaaca agatagagtg ctgtacatat caatggaatg actccggcaa tcacatacaa
    13981 tcctctaaat gaagtaagag attttagcat ccccatcaaa taaggaccaa cgcctaagcc
    14041 taggtctaaa ccaataaagt aggtagataa agcaatactg attcgatgtt cgcggacgat
    14101 ttttaaacaa acagcctgtc catttgacat aaacgtacca taacctaggc cgactaatgc
    14161 tccagagata agcaatgtca cactattcgt cgttacactc agtaaaaata atcctgctgt
    14221 taagaataaa tagctaggat acatcacgta ttgttctcct ttgacatcga aaatccttcc
    14281 agtcaatgga cgtgtaaatg tgatgaccaa agcatatacg acgaagaaaa atgacccagc
    14341 agcaactaag tggattactt ttgtataaga agataaaaat gataaaacac ttgaataaga
    14401 aagacccatc aaaaaggcaa ttcctgtaat gaaaagtgct ttcttttcaa taaaactatt
    14461 gatattccat gagcgaagag atattttttg ttcttcagat aatggaatat tttttacagg
    14521 aaagacaagg caggcaatcg taacaaagaa gaccagtaca attgaaaacc agataatgaa
    14581 acgaaaatca gtcatattca gcaaaatcat tcctaaaaat ggcccaacag cggcagctaa
    14641 actagtactt aatccataat aattgatgcc ttcccctttt ttagatgcag ggatatatgc
    14701 agtaacgata gcatttgtag ctgtagaaac tgtaccgtag ccgaatccat tcaagaatcg
    14761 aactaaatac aatgccccga tagttggcgt ataaagatat gctaatgttg aaaggagata
    14821 gaagatcgcg ccgcctctta aggtaaactt ccgaccaaat agttctaact gtttccccat
    14881 gaataagcgt gccagcaatg ttccgatgat atagatccca gaagcaaaac ctgcttggct
    14941 caagctggcg tgtaaggtgt cttgcgcaat gactgccatg atgaccatca acagataata
    15001 aatcaggtag acaataaaat tgatcaatgt gattccaata aaatatttat taaataattt
    15061 cttttccatg tatgttatac attcccctta agctgaatat gcttttaatt tttgtatttg
    15121 aacttctaca tcgtgataat tggatggata gcgatcaaca agttggcgaa taaataaatc
    15181 tcttgtagac ataaacagat ttaaaaattc ttcgcattcg tttgttggat tctcatccac
    15241 tgctttgatc aattcgatcg agttttgata cgcatcattt aaaaagacta aagaagcttt
    15301 catatcaaat cttgtttgat atgctgtttc taatcgatta atgattgtgt tcatccagta
    15361 attcatgttt tttccctcta ttcttaaaaa attttacttc tgcagtataa cgaataaaaa
    15421 aaaggaaaaa aatcgcataa gtgatggaaa aaggtactta acaaatgtta atatcattag
    15481 taaaatacct ttggacttta aaaagtttta aagtactgta tgatcttttt agatgaaagt
    15541 ttggatgggg gagcatcatt ggatagtaga gtcttaaagg aatatttgga taaaaatgat
    15601 tttccgatta tacgcaaagg atatcataaa tatttgacat tcgaagggat ggaagattcc
    15661 catacgtata tcttgaaaga gggaacggta aaagcaagtg ttatttcaaa tgatgggaga
    15721 gaatttaacc ttcgctatat caaagatctt gaaatcgtct ctcttttaaa agacgaatat
    15781 tcacaattca tcgattcgcc gtataatatt cgagttgaat cagaatatgc agagttttat
    15841 cgaatcaatc gtgtgcgctt ttggcaaaat atcaaccaag acccaaagct acaacactat
    15901 gtaaaagagt attaccgcta tcgtctgctt tattcgatga aaaaaatgca gcaaatgcta
    15961 ttaaacggaa agctgggagc tgtttgtacg caactttacg aattatatga tttattcggt
    16021 gtgcggacag aagaagggat gctgatcgat ttttcgatca cgaatgaaga aatcgccaaa
    16081 ttttgtggca tctcaactgc tagcagtgta aacagaattt taaaacaact gaaaaaagat
    16141 ggtattatca ccgttaaaga ccatcatatc gttattttga atatggactt gttagaagac
    16201 aatattatct tttgatgaac aaagaggttg tgaaatcagc gttttgacct gagtattaga
    16261 gaaacaaacg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
    16321 agctgattga acaccgttta ccaagaggtt gtgagcctgc cgtttaactc tgagtcacaa
    16381 ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg tgaccgagga
    16441 gttggtagga gaacaccgtt taccaagagg ttgtgaaaaa gctgttctgc atcaagtaat
    16501 aagaagagcc aagtaaaaac agtctcttat gtttttaact tggctggact tatcaccgta
    16561 gatgtaagct tttgaacgcc gtttatttgc aacaaaaggg gctgtgacac gatttttgtc
    16621 acagcccttt ttttgcgaac attcatttag caatttttag aaacttctgc cgccaccgcc
    16681 aaaagtcccg ccaccgctag aatgggtagt acttccgcca ccgccgcttc caggaggtgg
    16741 gcttttgggg attcttcttg tcgtgacaaa agaatttgtc agacgatcgg ttttttcagt
    16801 taatttgata ctggattttt ctcggaatgg atatttgtag atgcctgatt tcaattgata
    16861 acgcgaaaca gtcacaaaat aaaagacaag actaaggata agagccaaaa caacagcaat
    16921 gatgatttct actgtcgtca aaaccttata gcgtgtaatt ttgccagttt cttcgtctac
    16981 acgataatgt cctcctggaa ctccgtcttc cacatatgtt gctgatttag acaggtaagc
    17041 ttttgcagca ttgtaatagg cagcatttgt catctgatca taaacatcat ctaagatcga
    17101 atcgattctg ctatcagtca aataatcgat catattacca gaagtcgaaa tgtagatttc
    17161 acgctgtccc atatctaata gcaatactga gccattttga tcatttccga ctgcgtctct
    17221 taagtaatta tcagcaaagt ctcttggttc ttctgaatta gatgtagtcg ttacgatcaa
    17281 gactcgtcct ttgatttttt cattcagctt ttctgcctgt tgttccaaag attggatttg
    17341 atctgttgtg aataacccag cctcatcatt taccgtagct gtatctgcat gagtagccac
    17401 cggccagcca acacttacta gcaaaagaaa gaataaaaaa aattttttca tcatatcaag
    17461 taacctccga tcagaaagag gatcgctagc ccagcgaaaa tcccaagagc ggtcagtccc
    17521 aattttttct ggctgatcgg caggacaccg ctgacctttc ctgtttgtcc attcattgcg
    17581 tagtagtaga cttttttact tgagtcattg cttcgataag taacaagcca taccggcagt
    17641 aatacgtaat tatttacttc gtttgtgatt gaagcagaag ttcgcacacc ggtcaatgtt
    17701 gtatatccat ttgcggtatc ttttaacagc ttctctgaat agtcttttaa ttcagattga
    17761 atctgtgatt tgattgcctc atattcgata tctctttttt ctgcttggaa cccggctaaa
    17821 tactggcttt taaaatcaat tgctttatct aacgggaaag gttgcacgcc ttcgaccatc
    17881 ttttgttgcg tattttttga taaggcattt tttactagtt ctttaaaaga aagcttgcct
    17941 gctcttgaaa cagcaaattg cttcgtttct gtgtattcga tgtcacctat ccgccatatg
    18001 cggataactg taccattcgc ctgcatggca ccatctactt cagcatctgt agcccaataa
    18061 gggaaataca cacctgtcaa cttatcgatt tgatccttat tgaaaaaagc tttgggaaca
    18121 aaccattttt tcttggtcca agcgagaaat ctgttgattg cttcttcttt ctcgacagca
    18181 aaaggaagta ctttatttgg caagaattct ccgcttaaac gtcccgatag tacgacggga
    18241 ttatggcagt agtaacaata agtcgcagca gttgtcgcat cagtcacgat ctctgcacca
    18301 cagttgggac aaagaaaaag atctatcgct gcggtattat ctgcagattc gtccgctgta
    18361 tctgaaaaac cgcctgcctc gtcaaccgct ttgcgctcgg cttcattcat atcggcaagc
    18421 tgttcttggg ctgtaaaagt aaaatcttcg gtaggtgaag gttcttcttt gctttcttcg
    18481 ccagccatac gcgcttcttt ttgcttttgc tcatattggc tgacttcttc ttctgtatag
    18541 atattcaggc agtattcgca atgaaatttt tggtctttgg gatcaaaagt caacggaccg
    18601 ccgcaattag gacatttatg tgtaagaaca tccatcagct gcacaccttt ctaaataaat
    18661 taattaacgg ggattccttt aaacggttta ccacagtttg gacagaattt agggatgccg
    18721 ttggaaagat ccacgatctc attgcattca ctgcatcgca tttgtagctt aggctgaacg
    18781 ttttctacag gtttggcagc tccgcagttg gaacagaatt ttccggaatt ttctgtcccg
    18841 caaactggac atgtccaagt atccgcattt ccttgtgcag tttgttgatt ttgtttttct
    18901 gcttgttgtt tgatttgttc ttggttattt tgagctgctt gagaaagata agaaccattt
    18961 gcattcattc ccatacctac acccataaag ccggtcattg ctcctgcttc attttttccg
    19021 gctgcttcca taccacgtgc aatggaacct tgcacgtatc cttcacgtac gctaggatca
    19081 cctagcatcg ccccttcgtt acgcatattg atcagtttca cagaatcatc ggtgtaagaa
    19141 atgctggcta cagccacaga aacaatctcc atcccacgta attcacgcca agaatcatcc
    19201 aatgcagtat ccatgtattt gcttaattcc aaactcttag atggtacata agaaatccgt
    19261 tgaccatctg cagacatctg attgattgct gattgtagcg ctgttaggaa ttcagctaaa
    19321 tactgctcat tgatatcatt gatttccact tgtgttttgt ttttagggat tgcgtttgta
    19381 taaaataaaa tcgggtcaac gacatgaata gaataagtgc catgtgcccg taagaataat
    19441 tctgcgttat agaaattgtc aaagtagttc aacggcgcgc ttgtaccgaa tttgattccc
    19501 tttatttctt ggagattgat ataaaaaact tgttgtttct gcggtgtgac gccgccaaat
    19561 ttgaatcggt caaaagtttc agcaattgct tctttcagtg ttccattgaa catggaagga
    19621 gctgcgtcat ttttaatggt gtaataacct tcctcagcgg tataatcgat gatttttcca
    19681 ccatcgacca aaagcatcat catgtttgga cagacatgga cgactgttcc gtctgttaaa
    19741 acatcttctg ttcctttacg attggagcca cgtttgtcat ctttgcggac tttgacacct
    19801 tttgtcatga ccgttgtatc actcatattg tctggttcga tgacttctag ccattgatct
    19861 gccagaccgc cgccaaccat acttgttgct gctttgataa gacccataaa aaattcctcc
    19921 tctgattcag ctagtgccaa acaattattg tttatttatt ataccatagg aagatgtgaa
    19981 acttctcgga ctagagacgg aattgaaatg gaaaattgcg tattgtttaa atacctgaaa
    20041 aagccgagaa ggatagtagt ttttttacct aatatcacgt ataataagag atagtgaatt
    20101 ttttaaaaaa gggggatcct tatggcatat caagcacttt atcgagtctg gcgttcgcag
    20161 cgtttcgaag acatcgttgg acaaaaagct gtgacacaga cactaaaaaa tgctattgtt
    20221 tcccacaaaa cctcacatgc ctacttgttt acaggaccca gaggaaccgg aaagacgagt
    20281 gcagcaaaga ttttcgcaaa agcaatcaac tgtccgaaca gtaaagacgg tgagccatgc
    20341 aacgaatgcg aaatgtgtcg atcaattact gctggtacgc aagaagatgt gatcgaaatc
    20401 gatgctgcca gcaataacgg agtcgaagaa atccgattta tccgtgatcg ggcaaattat
    20461 gcgcctacaa aggcagcgta caaagtgtac attattgatg aggtacatat gctttcaaca
    20521 ggagctttca atgccttgtt gaaaacattg gaagagccaa aagaaagtgt catattcatc
    20581 ttagcaacga ccgaacctca caaaatacca gctacgatca tttcacggac tcagcgattt
    20641 gattttaaac ggatcaatac ggcagatatt gtcgaacatc ttgaatttat cttgaaagaa
    20701 tcgactatca gctatgaacc agctgcattg aatgtgattg cccgttctgc agaaggcggg
    20761 atgcgggatg cattgagtat tcttgatcag gcaatctcgt ttagtgaagg aacaattact
    20821 ttagatgatg ccatgcaagt gacaggtagc ttaactgacg aaatgatgga cagttacttg
    20881 agtgcttgcg tgaaaaaaga agtaccagaa gcattggaaa cattgaacca aattctcgca
    20941 gccggcaaag aatcacgtcg ctttctagag gatatgttac aatactgccg tgacttgctg
    21001 atgtatcagc aagcacctaa cctagttgag gaacaatcga gtacgatcag aagtacattt
    21061 acagaattgg cacaaactgt ttcagcggat cagctgtttt caatgataaa gattttgagc
    21121 gatactcaaa atgaaattcg ttttaccaat agtgccgcta tttatttgga agtcgctaca
    21181 gtcaaacttg cacagctacc gtctgcttct gttccttcag cagatgtagc atttgcagat
    21241 gatcagatag taaaacagct acaacaagag attaaggaac taagaaacga aatcaaggaa
    21301 ctacgcagta atacaggaat tgagaataca aaagtacagc cggtacagca aacaaatcat
    21361 aaaaaacaga caagtaccta tcgtgtaccg acggaacgag tatataaagt tctgcgggaa
    21421 gcgacaagac agcatctgaa ccaagtaaaa gaagtatggg aagacttgct gatgagtcta
    21481 tctgtcactc agcgagcaat gcttaaagca agcgaaccag ttgcagctgg acctacagga
    21541 ctagtgattg cgtttgatta tgagattgtt tgtcaaagag cagcaaatga cgaagaattg
    21601 cagctttcgg tacacaacaa cttaagccga atgatcaaag attatgcgcc tgatcctgtt
    21661 tatattacaa gagaaagctg gccagaactt cgaagaaatt atttgacgca ggcacaagaa
    21721 gagggaacag aaaactcttt tgcttctgaa gactcagaag gagaaatcga gttgcttcct
    21781 caagaacaag aagtaccaga agaaacactt gtagtaaaag cagaggaact gtttggctcc
    21841 gaagctgtta cagtggtaga tgattaataa aaaggagtag ataattatga tgcgtggaat
    21901 gggcaatatg caaggcatga tgaaacaagt acaaaaaatg caaaaagaaa tggcagaagc
    21961 acaagagctg ctaaataaaa cagagtttac aggtaaagcc accaatgaat tggtcaaagc
    22021 ggtttttaca ggcgatcgtc gcatgaaaga tatccaaatc aatgaagaag tcgttgatcc
    22081 tgatgatatc gagatgcttc aggatcttgt cgtaatggca gtcaatgatg cgatcgcaca
    22141 aattgatgtg gaaactgaga aaaaattagg gaaatacgca aaaggtttgc caggatttta
    22201 gttttaaact gaaggagtaa tgaatatgca atatccagaa ccaattgcga aactgatcga
    22261 cagctacatg aaacttccag gaatcggtca gaaaacagcg acaagactcg ccttttatac
    22321 gatcgatatg aaagatgaag tagtaaatga atttgctaaa tctttgttaa gtgtaaaacg
    22381 cgatttgcat ttttgtagta tctgcggaaa catcacagaa gaagatccct gcgaaatatg
    22441 tagaaatcct tcacgtgatc gaagcgttat cctagtggtc gaagagccga aagatgtaat
    22501 ggcaatggaa aaaatgcgag aatatcacgg actttatcat gttctccatg gtgtgttatc
    22561 accgatggaa gggacaggtc cagaagacat caatatttct tctctgttgc agcggctgca
    22621 tgatgcagat gtaaaagaag tgattatcgc aacaaatgca acaacagagg gagaagcaac
    22681 agctatgtat ctctcacgtc tgatcaaacc cgcaggtatc aaagtgactc gtctagcaca
    22741 tggtctgtct gtaggctctg atatcgagta tgcagatgaa gttactttat tgaaagctgt
    22801 cgaaggaaga cgtgagttat aaatttctaa ttaagtgttt tttcagatac aatataaaag
    22861 cagcacaaga gatgggggca acaatgtgaa cggactattt attacaatcg aggggcctga
    22921 tggtgcagga aaaaccagca tcttgaatga actttaccct cttttgaaag aagtagcaaa
    22981 aacagaaatc attcaaacaa gagaaccagg aggcattccg attgctgaag agatccgagc
    23041 agtgatcctt gatccaaaaa atgatcggat ggatgaacgt acggaagcac tgctttatgc
    23101 agcagcaaga agacaacatc tagtcgaaaa ggttctgcct gctctggcaa aagggaaaat
    23161 cgttttatgc gatagatttg tcgacagttc tcttgcttat caaggtgcag gccgacgcat
    23221 cggcgttacc gagattgctc ggttgaatga gtttgctaca gaaggtacta cgccggattt
    23281 taccctttat cttgacgtag attcagacac cggattaaga agaatcaaga aaaacagaca
    23341 aaatcaaatc gaccgtttag attcggaagg tttggaattc caccagcgtg tacgtcatgc
    23401 ttacttaaag ctggcagaag agaatccgga aagaatccac aaagttgatg caagaaaaag
    23461 ctttgaagaa gtgctgcaaa caagttatca tacaattatt gaacagtatc cgcaattttt
    23521 tgaaaactag gaaggtgaac gtttatgaaa attattttag cgattatcca agacaaagac
    23581 agcaaccgtt tatcaaatga attgattgat gcaaatatcc gtgccacaaa actatcatcg
    23641 acaggcggct tccttaaggc tggaaacagt acgtttatcg tgggtattga agatgaccgt
    23701 gtagaagaag cattggaaat catcaaaaaa acgtgtgaat caagaaagca atttgtatct
    23761 acacctgtta cactggatat ttcgatggat gggggcgtcc cttatccagt agaagtagaa
    23821 gtaggcggtg ccacagtctt cgtcttacct gttgaaggat tccatcaatt ttaggaggag
    23881 ctgagctatg aatcaagaaa taaacttggc tcaaatgcaa ccgatcgtgt atcagcagct
    23941 tcaacggagt tttgagcatg ggcgtatcgc tcatgcttat ctttttgaag gggagaaagg
    24001 aacaggaaaa catgaagtag gtatatggct tgcacagcac ctgttctgta cccaaatgaa
    24061 agacaatctg ccttgtggaa aatgcaacaa ttgtcagcgg atcgaaaaga aagaacatcc
    24121 agatgttttg gttattgaac cggaaggaca aaccatcaaa gtcgatcaga tcagacgttt
    24181 gcagacggaa ttcagtcgca gcggttacga atcaagaaaa aaagtttttt tgatcaaaga
    24241 agctgaaaaa atgaatagca gtgcggcaaa cagtttgttg aaattcttgg aagagccccc
    24301 tggagatttt ttagcggtgt tagaaacaga tgcagtagga agaattctcc ctactattca
    24361 atctcgttgt caagttctgc attttcaaga actttcgaaa gaagcactga tccatcgatt
    24421 gcaagaagag cagattccat tggaaaaagc aaaacttttg gctttcttaa caaacagttt
    24481 aggaaaagca gttgaaatct ctcaagatga atggtttaat gatgctaagg acttgattta
    24541 tcaatggttc gtttacttgc aaaagcaaga tactcaagcc tttatctatg tccaaaaaaa
    24601 attggtaaaa tcatttaaag aaaaaacaca acagtttaca ggattatctg tcttgatgtt
    24661 ttattatcag gaagcacagc gtcaagcagt agcaaacggc gaatatggca aagtgaaaca
    24721 gataaaccag ataattgaac ggatattgat cgcagagcag aaactacgca gcaatgtcag
    24781 ttttcaggct gttgcggaac agttcgtttt gcatacgatt ttttagcttg ccgcaaagga
    24841 tgatgatcaa ggaggatgga aatggtagaa gtagtaggtg tccgatatag agatgcaggt
    24901 catatctatt attttgcacc aggagactct gaatatgttt atagtgacaa ggtattagtc
    24961 gaatctcagc aatccttaca tattgctact gttgtccttc ctaaaaaaga gattgatccc
    25021 aatgaccttc ctgaagctat ccgtccgatc ttgcataaag cgtcacaagc tgaattagaa
    25081 aaagacaaaa aaaacaaaaa agatgcttat gatgcattga aaatcgcaaa agaaaaagtg
    25141 cagttgcgtg agttggaaat gaaaatggtc aaagccgaat acacatttga tcgaaataaa
    25201 ttgatttttt ctttcacagc ggacgggcga attgacttta gagaattggt aaaagatctg
    25261 gccgctgtat ttcgtactcg tattgagctt cgacaaattg gtgtccgcga tgaagcaaag
    25321 ataataggcg gaattggtcc atgtgggcgt ccattatgct gttcttcttt tttaggcgat
    25381 ttcgtgcctg tatcaatcaa aatggcaaaa gatcaaagtt tatctttgaa tccaacaaaa
    25441 atttccggtt tatgcggacg cttgatgtgc tgtctgcaat atgagagcga agaatacgag
    25501 acagcgaaaa aagaactccc tgactttgga aaagtaattg ctacacctga tggtaaaggg
    25561 aaagtcgtag ggttgaatct gcttagccgt atcgttagta ttcggctgac tggacgggaa
    25621 aatcctgtcg aatatgaatg ggatgaattg aaagaattta tccaagtagg tgagatgaat
    25681 ggataagaga tcattatacg acggattgaa tcaattagaa tcagagttga gaaatacatt
    25741 aacagaactt gttgaaatga agcatgcttt gcatgagatc gtagaaaaaa atacaacttt
    25801 ggagatggaa aatcaacgtc tgcgggaaca cctgcgggaa ttgaaccaag ttgcacaagc
    25861 tccagctgaa aatgtgaagc aggaactttc taagtctcgt atgaatcttg aaaaaattta
    25921 tgaagaagga ttccatgtct gctacgattt atatggatca agaagggaca atgacgagcc
    25981 ttgtgcgttt tgtctagaag tgatctatcg cgaaagcggt cgatgaccaa taacaagagg
    26041 agactggagc ggagaaat
//
LOCUS       DAZUMM010000034        25695 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000034 DAZUMM010000000
VERSION     DAZUMM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.034"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            376..2436
                     /locus_tag="ACG8E6_001359"
     CDS             376..2436
                     /locus_tag="ACG8E6_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFS5061784.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSNVYEKDYSQFEGKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2519..3088
                     /locus_tag="ACG8E6_001360"
     CDS             2519..3088
                     /locus_tag="ACG8E6_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5061785.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3263..4069)
                     /locus_tag="ACG8E6_001361"
     CDS             complement(3263..4069)
                     /locus_tag="ACG8E6_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061786.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4207..5721
                     /locus_tag="ACG8E6_001362"
     CDS             4207..5721
                     /locus_tag="ACG8E6_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFS5061787.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(6124..7107)
                     /locus_tag="ACG8E6_001363"
     CDS             complement(6124..7107)
                     /locus_tag="ACG8E6_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFS5061788.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(7112..7759)
                     /locus_tag="ACG8E6_001364"
     CDS             complement(7112..7759)
                     /locus_tag="ACG8E6_001364"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFS5061789.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7844..8476)
                     /locus_tag="ACG8E6_001365"
     CDS             complement(7844..8476)
                     /locus_tag="ACG8E6_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5061790.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8492..9043)
                     /gene="coaC"
                     /locus_tag="ACG8E6_001366"
     CDS             complement(8492..9043)
                     /gene="coaC"
                     /locus_tag="ACG8E6_001366"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFS5061791.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNTITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(9040..9777)
                     /locus_tag="ACG8E6_001367"
     CDS             complement(9040..9777)
                     /locus_tag="ACG8E6_001367"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFS5061792.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9774..10763)
                     /locus_tag="ACG8E6_001368"
     CDS             complement(9774..10763)
                     /locus_tag="ACG8E6_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS5061793.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            11021..11563
                     /locus_tag="ACG8E6_001369"
     CDS             11021..11563
                     /locus_tag="ACG8E6_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS5061794.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11580..12665
                     /locus_tag="ACG8E6_001370"
     CDS             11580..12665
                     /locus_tag="ACG8E6_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5061795.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12677..13483
                     /locus_tag="ACG8E6_001371"
     CDS             12677..13483
                     /locus_tag="ACG8E6_001371"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5061796.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13484..14311
                     /locus_tag="ACG8E6_001372"
     CDS             13484..14311
                     /locus_tag="ACG8E6_001372"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5061797.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14308..15381
                     /locus_tag="ACG8E6_001373"
     CDS             14308..15381
                     /locus_tag="ACG8E6_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5061798.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15809..18505
                     /locus_tag="ACG8E6_001374"
     CDS             15809..18505
                     /locus_tag="ACG8E6_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5061799.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18570..19070
                     /locus_tag="ACG8E6_001375"
     CDS             18570..19070
                     /locus_tag="ACG8E6_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5061800.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            19082..19576
                     /locus_tag="ACG8E6_001376"
     CDS             19082..19576
                     /locus_tag="ACG8E6_001376"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5061801.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19597..20406
                     /locus_tag="ACG8E6_001377"
     CDS             19597..20406
                     /locus_tag="ACG8E6_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5061802.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20403..21224
                     /locus_tag="ACG8E6_001378"
     CDS             20403..21224
                     /locus_tag="ACG8E6_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5061803.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21275..23374
                     /locus_tag="ACG8E6_001379"
     CDS             21275..23374
                     /locus_tag="ACG8E6_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFS5061804.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23371..24489
                     /locus_tag="ACG8E6_001380"
     CDS             23371..24489
                     /locus_tag="ACG8E6_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS5061805.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24528..25406
                     /locus_tag="ACG8E6_001381"
     CDS             24528..25406
                     /locus_tag="ACG8E6_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5061806.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25552..>25695
                     /locus_tag="ACG8E6_001382"
     CDS             25552..>25695
                     /locus_tag="ACG8E6_001382"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS5061807.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFI"
BASE COUNT         8142 a         4471 c         5428 g         7654 t
ORIGIN      
        1 ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa taccatcatc
       61 agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat ggcagctttg
      121 tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact agatataact
      181 agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat tattccgatt
      241 ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct tcttttgatt
      301 ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga caagagtagg
      361 gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa acacgcttgt
      421 cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat gatgcgcacg
      481 atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc aacgacacaa
      541 accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat cactattact
      601 catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc ctataataaa
      661 gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac attatggaca
      721 tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga tcctacaaaa
      781 ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga tataaaggac
      841 ggtggaagcg cacaagtttc taatgtatat gaaaaagatt attcacagtt tgaaggaaag
      901 aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga aaggctgaag
      961 aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg gaatatcgtg
     1021 gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc aattgcttta
     1081 aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc tatcaaagga
     1141 aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt tgaatatgta
     1201 acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg gacagtagat
     1261 actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa aacatcaaat
     1321 gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc gactgtcatc
     1381 actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca tatgagtagt
     1441 gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt gccaaatata
     1501 gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg gatgcaagca
     1561 aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca ctcagaactg
     1621 atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta tataccagac
     1681 aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat aaacaaagaa
     1741 gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa ggatgtatcg
     1801 gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac aggtgatttt
     1861 acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc tcagtcacta
     1921 ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag acaagaagaa
     1981 agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg gaaagtaaaa
     2041 caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc taataaaacg
     2101 ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga aattttagag
     2161 cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga agatcagaag
     2221 gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga ttccaccgat
     2281 gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc tggtggaggt
     2341 attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg ggtatacgct
     2401 aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc tgttatagtc
     2461 aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg atagaaaaat
     2521 gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc aacaaaatgt
     2581 tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag aagatgaatc
     2641 tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg aagcacttga
     2701 gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga tgttttttgt
     2761 agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa aatcaagaga
     2821 gcaagtgtat ccggtttttc attggttagc agtgaaacca gattaccaag gaaaagggct
     2881 cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata cggacggacc
     2941 aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata aaaaaatggg
     3001 ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg aaaaagttat
     3061 gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc agtattggga
     3121 taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact atcggataac
     3181 taatggcatg ccatacattc aaaaaagaag gggcagtgac aaaagtattg tcactgcccc
     3241 ttctcttgtt ttttagcatg ttttatcgtt tgattttttt aggaataaaa ttcttgatga
     3301 acgtaaattt ttggtaatcg tttcgactat agaggtaacc aaatacactg aaaacaactg
     3361 ctccaataaa attaacaaat aaatccttca tagtatccat gattcccacg tctaagtaac
     3421 cattttggat gattgtttta tctgttttac catctgcatt ttgactttca atgattgtcc
     3481 gagtgatatc tggaacatga tagacagtgt ttccgtcttt acttaactcc acactattga
     3541 tttgctggat gatcgtatct ttttgcatat cgaattgcag taatttatct gctgaaaatt
     3601 ccacaaactc ccacataaca ccaactgtca tcgaaaaaca gaaggaaaca actgctagta
     3661 ataaaggagt caagttcatg gtttttgtat ttttattgag caaataaaca agggaaaagc
     3721 cgacaccccc agctaaaaat ccgctgaatg catgtagaaa agtatcccac attgaaaaat
     3781 agccataaaa atcacttaac tctcctaaaa ctgttgaact aaaaataaaa gataacaaaa
     3841 tgatttctag catatttgga atctgtatct ttaatatctt ttccagaaaa taagggattt
     3901 caaataaaat taatgtcagt aataaaagca ttgtatttcc ccagtgttgg ttccagacct
     3961 gatccactag cataataacg acgatgatac gtaataaaat atagattccg tttgtgattc
     4021 tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa atgattcata aaataactcc
     4081 ttcatgtata tccttaatta tactaagaag cgtgtgttta ccaaagctat atgacttaaa
     4141 gaaaaacaga gttgatattg tgaaaaaaat cacttaatat agtataagga gaaaaggggg
     4201 atacaaatga aaaaattagt tgctggttta ttaacagccg ttgcacttct tgctatgagc
     4261 gcatgcggcg cagatcagaa aaatacagcg cagacaagtt ctagtacgaa ggaatctgcc
     4321 gaagtatcat caggcgcttc tgaacaagta tatacagatc caaaagaaat gaaagaatct
     4381 tatgatgtcg tgattattgg agctggtggt gcagggatgg cagcagctat cgaagcaaaa
     4441 gatgctgggt tgaatccagt aattttggaa aagatgcctg ttgccggagg aaacacatct
     4501 aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc aaaaagaaga aggaatcaaa
     4561 gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg gtggaggaac gaatgataaa
     4621 gaaatgcttc gatactttgt cgatcactct gctgatgcgg tcgattggct agatcaaaac
     4681 ggtatcaaac tggacaactt aacgatcact ggagggatga gtgaaaagag gacacatcgt
     4741 ccgtctgatg gctcggctat tggtggatac ttagtggagg ggttattgaa taacgtccat
     4801 gagcgggaaa tccctatttt tgtcaacgca gatgtgacag acattaagaa aaatggggaa
     4861 acggtcaatg aagtgactgt acatgttcaa ggagaagaac caaaagaggt caccggaaaa
     4921 gcaatcgtag ttaccacagg cggttttggt gccagcaaag aatttattga aaaatatcgt
     4981 cccgatttag ctgattatat tacgacaaat caagaaggat caactggaga tggtatcacc
     5041 atgattgaaa aattaggtgg acaaaccgtt gatatggata aaatacaaat tcatccaact
     5101 gtgcagcagg atgagggggt cttgattggt gaggttgttc gcggagaagg agcaatcttg
     5161 gtcgatcaag aggggaaaag atttgtcaat gaaatggata caagggataa agtatctgca
     5221 gcaattacag cattgcctga aaaatcagct tatttgattt ttgaccaagg cgtgcgtgat
     5281 cgtgccacag ctattgaatt ttatgaccaa aaaggatatg tcacaactgg aaagacaatg
     5341 gaagaattag cagaaaaaat cgatttacca aaagaaacac tagcaaaaac agttgatgga
     5401 tggaatacgg atgttgcgga gaaaaaagat gctcaattca accgtgccac agccatggaa
     5461 catgacttat cgtctccgaa ttattatgcg atcaaaatcg caccaggaat ccattacacg
     5521 atgggcggtg tgaaaatcaa tacgaacact gaagtattag ataaagaaaa ccagccaatt
     5581 agggggttat atgctgctgg tgaagtcaca ggtggcttac atggaaacaa ccggatcggt
     5641 ggaaattcag ttgcggagat cattattttt gggcgacaag cgggcataca agcagctcga
     5701 tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa gaggttgtga aatcagcgtt
     5761 ttgagctaat accgagggtc agctgattga acactgttta gtgaagaggt tgtgaaaaag
     5821 ctgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg tttttaactt
     5881 ggttggactt attaccgcag atgcaagctt ttgaacaccg tttattcgga aaaagagcgt
     5941 gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca
     6001 gaagaaattc cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca attttcgtga
     6061 atttcttctt atttcttgga gttaaacgct tctgtcccag cctctttttt gatcagcatg
     6121 attttattca gttatcttca aaatgatttt tccgatgttt ttgtttgctt ccatatattc
     6181 atgcgcttgt ttgactgcgc tgaattggaa tgtacggtca atgattggcc gaatcttgcc
     6241 attttctaga aaaggcccag ctttttgcat aaaagtttgt gcgagctctg ctttatactg
     6301 atcacttcta ggtgtcaata atgtaccaag cagttggaca cgtttcccaa tcaatacacc
     6361 aaggtctatc tcaggcacga tggtcccacc tagcatacca atcaagaccc aacgtccatc
     6421 aacggctata gaagctaaat tcttttccca gtaagaagcg ccgatgaaat ccaaaataac
     6481 atcgactccc cgaccttcag tgatttttcg tacctcttct tgaaagtctt gttttttata
     6541 gttgatcaat tcatctgcac caagttcttt acaaaaggcg agtttttctt ctgatcctgc
     6601 agtaacgatg attttcgcgt gagaaagctg tctagctaat tggatcgctg ctgttcccac
     6661 accacttgct cctgcatgga tcaagacagt ttcgccttct tgtaattttc caagccagta
     6721 aagtgtttga taagcagtca aaaaaacttc tgagattcca gcagcagaaa catagtctag
     6781 cgtatcaggg agaaggattg cacgatcggc aggcatcaca gcatattcgg cataaccacc
     6841 tagattgact aatccgtata cttttgtccc aggagtaaaa gtggagatat cacttttatt
     6901 ctcaactacc tctccagcta cttcgactcc caaaataggg tagggaggtt tcaaagagac
     6961 tgattctctt ttcataatat ctgttcgatt gacagctgct gtatggactt tgatcagtaa
     7021 ttcgccttgt tttggtttag gaacaggcac ttcaatttgt tctaatacat ctacagaacc
     7081 aggatgcttg attgaaatgg cacgcatgga tctattcctc ctttgcccat tcttggaatg
     7141 ctgtattcaa ctctttgcta ccttcagaaa gtactagagt cgtacgagaa atatccgttt
     7201 cttccactag agcggcaagt acagcagcaa ctgtagagcg agtgatcgtt tttgatgcct
     7261 tctcattagg cttagttaat tgaatggaag taacttcttc atcgttagtc aatgtgactg
     7321 gttgaagaat gacaaaatct aagttagttc gtttcagcca ttcatctgca taatgtttag
     7381 taatataata atctgtcatg gactcaggcc attttgctgg ttcatctgca taaacagcac
     7441 tgaccatcag atagcgagag acatgcgcat tttcagctgc gatcactgtt ttgattgcgc
     7501 catccaagtc tacttgcaga agattctttc cttgagagcc ggcagcaaaa atcaatacat
     7561 cgatgccttt gaatgcttcg gtcatttttt ccacggacca agttaaatca aaagagacat
     7621 acgaaacatt ctcttttttt actaatgttt gggaagcaac atctctcacc cctgcaaaaa
     7681 tttcatggga acttggagct aaatctttta tcaggtgtcg gccaatttgt ccgtttgcac
     7741 ctacaacaaa aattttcatt tggatcatcc tttcgacgtc aaatctttcc attcaatagt
     7801 aacaccaaaa gagaaaaaaa tcagagatcg gctacgatga ctatcagtta gtaagagaag
     7861 ggtgcttgaa gtatcttaac aagacattgc atactgctgc agtcgcaaga cctgcgacga
     7921 ttccttcagg tataccactt gtaaaaataa ccgctagaat agctgaataa acagcattta
     7981 aatttgttcc gatgactttg gcataatctt gctggaataa aaagtagatc atattcatca
     8041 ctaaaatcgt attggtcaat gaaccggaaa gtccagctaa gaataaagaa acggaatctg
     8101 ctttttgttt cattagtttg cgcagacctt tataaacgaa ataaggaacc accccaatca
     8161 agatgcgagg aataaacgcg atcagcaagg ctttccagct tccatgttct gttccgatga
     8221 ctgggataaa cggagaaaag acaaaagaca aaggtgtttg gataactgtg ctacggatca
     8281 tgctgatcag tccaaatgta ccgccaagaa aagcacctag tcttggtcct aaaacgatcg
     8341 aagcaatgat tacaggaata tgcatggttg ttgcattgat tggaccgata gggataaaac
     8401 ctaaaggcgt aacagctaat aaaatcaaaa tagctaaaaa catcgcagtt aatgtaaagt
     8461 tttttgtatt tttcataagt actccttttt tctaagcatt tctttcatta agagtcagca
     8521 tgacctgttc gatgatcgtc tgaatatcag ctaaggcacc ttttccaaaa tctccacaag
     8581 ctaaaagaga ttctcttggt tcaatttcct gatagccgac agatctcaat gtttccagat
     8641 tacgctgagt gatcgtgttt tgatacatat tcgtgttcat ggcaggggca atcaattttg
     8701 gtgttcccac tggaagcgcc attgcgactg ttgaaagaag atcgtctgca atgccatttg
     8761 ctagcttgcc gataatgttt gctgtagcag gcgcgatgag aaacaagtca gcttgttttg
     8821 ctagttcgat atgattgatt acctgcggat ttgattcttg catgacgtct gtatggactg
     8881 cgcgcttcga cagagattgc aaggttaatg gcgtgataaa atgtgtgctg cttttagaca
     8941 taatcacatc tacctgggca cctaatttga cgaattgact tgtaagatca gcgcttttgt
     9001 aagcagaaat acttccagta acgcctagta agatcttttt cattttgatt cctccagtct
     9061 aattttctca gtaatcaatg cagcaatctc agatttactt tgagcttctt ccactgttcc
     9121 atctttagat aagagatagc cgtggtgtgt ctcgccatgg atttccgtca aatcatttgc
     9181 gaaaataaaa tcagttttgt tatttatcag actatttttt gcgactgcca gaagagattc
     9241 ctttggtaca tctactaata gtttgaaacc aatgattttt gcttttggct gccattcacg
     9301 tagataagaa atgatttttg gtgttttctt caaaacgagc acaagatgct cggtgttaga
     9361 agagatcttt gaaacagtgg tatctgtctg ttcattttcc gcaaaccaat tgtccagttc
     9421 ttcttgcgtt gaagaagtgg gtaggttttt tgccagctgt tcttctgaaa aactaaaagc
     9481 aggagtaaaa tcactgactg ccatactgtg gataatcgca tcgtagtgtt cttttttcgt
     9541 tagttcttct aattgtaaaa ataagtccag tgcagattcg atcgtataaa tcgttatatt
     9601 ggagtgtctt ttcggtttca gtgcttgttt cgtagtcacg taatcgattg tggtatccgg
     9661 attggcagct aaataatcag ctaatgcttg ccctaatcgt cctgtcgaat gatttgtaat
     9721 agcacgtact tgatcgattt tttcagtcgt tcctccagct gtgattagaa tcttcattta
     9781 tggtcaccta catttactag tgttcttcct tggtgtgttc cagcaagtag tttagatgct
     9841 gtttcaggca attgatccag agtaatctct tggacgacca gttcatctgc aatctgcaaa
     9901 tccgccatct gttgccataa agaaagacgt tgatctattg gtacattgac agaatcaatt
     9961 ccaatcaact ggatgtttcg cagaataaaa ggcaagacag tcgcgtttag tttgattccc
    10021 ccagcattgc cacataaagc tactgcaccg ttataagaga tcaatggaag aagactgctt
    10081 agctgctcgc cacctactgt gtcgatgaca tagtcgattt gctgttttcc taaagctttt
    10141 gttgtctctg gcagaaattc atcaggtcgt acgacttgca aagcacctag actttttagc
    10201 catgtgacag actcttcttt tctggaaaag gcaatgattg actggaatcc tagtttatgt
    10261 aacagggcta tcgctgtact gcctacaccg ccagaagcac cagtaacgac aactttttta
    10321 tcggaggtca taccttgacg gattaaagcg ttcacacaca acatagcagt aaaccctgct
    10381 gtaccaagga tcatcgcttg acgcagcgtc atattttttg gtagaggaac taaccagtct
    10441 cctggtactt tttgatattg gctataaccg cctggatggg tgacgccaag cccaaaacca
    10501 gtagcaagta caagatcttc ttttgaaaaa cgtggattgt tactttggac gacagttccg
    10561 gataaatcaa tccctggagt catcggatat gttcgtatca cctgaccaga ctcgctcatt
    10621 gccaatgcat ctttataatt gacatcagaa taggcaattt ttaccaagac ttcattttca
    10681 gccaactcag taaatggcag atcactgatt gctgtatgga aaacaggttc ctttattacc
    10741 tgaaaagatc gaaaagcgtt cattcgtgag acactccttt taaaattttc ttttatcata
    10801 ccataaagcc tactctttta ttcttgttct tccgtaaatt tagacaaaaa ttaggtgaaa
    10861 aataaagaat caaacaacta gaatttattc caaaagataa agaaatattc agcaaaaaaa
    10921 attttacaaa gggttgcctt tttcaataaa tttcgtatac taatacgagt ggtagtaaac
    10981 aggaagttta ctattagaaa acttcgattc ggaggaaatt atggaaattg gtgagaagtt
    11041 aagaaatttg cgcatccaaa agaatttgac acaagaagaa ctaggcgaac gcacagattt
    11101 gtcaaaaggg tatatttccc agttggaacg agatttaagt tcaccatcaa tggaaacatt
    11161 cttttctatt cttgaagtct taggtgtgac tccagaagaa ttttttcatc aagaagcagc
    11221 gaatctgcaa gtggtttatt caaaagaaga tcatacggtt tatttcgatg aagaaaacgg
    11281 gtatgaattg gaatggctcg tggctgattc gaatgaaaaa gaaatggagc ctgtcttgct
    11341 gacttttgaa ggtgcaggag aatataagac gtttgaacct tcattatctg aaacgtttat
    11401 ttttgtatta gaaggggaat tagagttaag cttaggagag acggtctatg tggcaaaagc
    11461 aggacagtcg atctattatc aagcgacaaa acaacatcaa ttaaggaatc atctcaaaaa
    11521 acgaacaaaa gtattgattg ttgcaacgca atcatatttg taatataggg ggtaaaaacg
    11581 tggggaaacc aatcatttca tttgatcaag tagtgaaacg ctatgatgat gaaacagtat
    11641 taaaaaaagt aagttttgag atcgaacaag gaaaatttta tacactgctt ggtccttccg
    11701 gctgtggtaa aacaacgatc ctacgtatta tagctggatt caccgaagct acagaagggg
    11761 acatctattt tgaagggaaa cggttgaatg atttgccagc taataagcga caagtaaata
    11821 cagtcttcca agattatgca ctttttcctc atatgaatgt ttttgataac gttgcttttg
    11881 gattgaagat caagaaaatg tccaaatcag atatcgaaaa gaaggtaaaa gatgctttgc
    11941 gtatggtcca gcttcctgga tacgaaaaac gggagattag tgaaatgtct ggtggtcaaa
    12001 gacagcgtgt agcgattgca cgagcaattg taaatgaacc gaaagtattg ctattagatg
    12061 aaccgttatc ggcgcttgat ttgaaactac gtacagatat gcagtatgaa ctgcgggatc
    12121 tgcaacaacg attaggaatc acgtttattt ttgttactca tgaccaagaa gaagcgttgg
    12181 caatgagtga tgagattttt gtcatgaata aagggaaaat cgtccaaagt ggtacacccg
    12241 tagatattta tgatgagcca atcaatcatt tcgttgctga ttttgttggg gagagcaata
    12301 tcgtggacgg tgtaatgatc gaagataatc tagttgagtt tgttggtaag caatttgaat
    12361 gtgtcgatgg cgggatgcgt ctaaatgaac cagttgaagt tgttttacgc ccagaggatt
    12421 tgacgatcac gacaccagat aaaggaaaac tagtcgtaac ggtcgatact cagctgtttc
    12481 gtggtgtcca ttatgaaatc atctgttatg atgaacagca aaatgaatgg atggtccatt
    12541 caacgaaaaa agctaaagaa ggaagcaaag taggattggc ctttgagcca gaagatatcc
    12601 atgtcatgcg tttcaacgag tctgaagaag aattcgatgc acgtcttgac agctacgaag
    12661 aatagggggg acaagaatga aaacaatgcg caggatctat tccgtccctt atattttgtg
    12721 gctcggttta ttcgtgatcg cgcctgtatt aatgatcatt tatcaatctt tttttgatat
    12781 gaatgggcaa ttcacgctga ataattatct atcttatttt acgtcgggga catcgctttc
    12841 tatgacgatc aattctgtat ggtatgcatt tttgatcacg tttttcacgc tgatcatcag
    12901 ttacccgact gcttattttt taacaaaact gaagcacaag caattatggc tgatgctgat
    12961 tattttgcct acatgggtga atctgctgct taaagcttac gcatttattg ggattttcag
    13021 tgtgcacggc agcgtgaatc atttctttga ttttatcggg atcgggccga aacagatatt
    13081 attcacagac ttcagttttc tgttcgtggc aacgtatatc gagattccat ttatgattat
    13141 gccaattttc aatgcattgg aagagatgaa tccttctttg atcagtgcta gtagagattt
    13201 aggcgcgaat agctttgaga ccttccgacg agtgattttt ccgttatcat taaatggtgt
    13261 gaaaagtggt gtccaagcag tatttattcc ttctctttct ttattcatgc tgactcgtct
    13321 gattggcgga aatcgggtga tcactttagg aacggctatc gaacagcatt tcttggttac
    13381 acaaaactgg gggatgggtt ctacaatcgg tgtgatttta atcattgcta tgttcatcgt
    13441 gatgctcctg acaggcgaga agaaaaaagg aggtcgctca taaatgaaaa aatttaaatg
    13501 gtcgtatctt tatttaggga tcgtcttttt attcttatat gcaccgatct tctatctgat
    13561 tttttattcc ttcaatgaag gaggaacgat gaacagcttt acaggattta cttgggaaaa
    13621 ttatgaagct gtttttgaag atacacgttt gatcattatc gtattgaata cgttcatgct
    13681 ggctttctta tctgcattga tcgccacgat catcgggaca tttggggcta tgggaatcta
    13741 ttacacgaaa aaacgacgtg cacggacagc gttgttgagc atgaataaca tcttattagt
    13801 ctctcctgat gttattatcg gtgctagttt cttgattttc tttacactac taggattcgg
    13861 attaggtttt cagagtgtat tgctttccca tattgctttc agcattccaa tcgtcgtctt
    13921 gatggtcttg ccaaaattgc aggaaatgaa cgattcaatg gtcgatgcgg ctagagacct
    13981 tggtgctaat aatttccaag tagtcaaaaa tatcattttg ccttatcttt ctccaggaat
    14041 catagctgga tattttatgg catttaccta ttctcttgat gattttgcgg ttacattttt
    14101 cgtcacagga aatggcttta cgacgctctc agtagagatc tattctcgtg caagacaagg
    14161 aatcagttta gaaatcaatg cgctaagtgc gttagttttc ttgttctcca tgatattggt
    14221 cattggatac tactttatta gtaaagaaaa ccgtccaaaa cggctgaaga aaaacagaag
    14281 agtacgcgag ggggtggcac ggctgcgatg aaaaaattac aatctctgtt tttaggtatt
    14341 ctcatcatta ttgcggtgct gctgcttggt gttcgtcaac ttgaaaaaac aagtggcatg
    14401 agtggagcaa aggtacttac catctataac tggggagatt atatcgatcc tagtttgatc
    14461 acgaaatttg aaaaagaata tggatacaaa gtgaattatg aaacatttga ttctaatgaa
    14521 gcgatgttca caaaaatcca gcaaggtggg acaaactacg acatcgctat accaagtgaa
    14581 tatatgatcc aaaaaatgat caaagaaaaa ctagtacttc cattagatca ttcgaagatc
    14641 aaaggattaa ataatattga tccacgattt ttagacttgg attttgatcc agataatcag
    14701 tattcgattc catatttttg gggaacttta gggatcgtat ataacgataa attctttgat
    14761 gcaaatcaaa ttaagcattg ggatgatttg tggaagccgg aattaaaaga cagtttgatg
    14821 ctgattgacg gtgcacgaga agttatgggg ctttctttga atagtttagg ctattctttg
    14881 aatagtaaga atatggcaca gctgacagaa gcagcaaata aattgaatca tttaacacca
    14941 aatgtcaaag caatcgtggc agatgagatc aaaatgtata tgatcaatga agaagcctcc
    15001 gtggcagtga cattttctgg cgaagctgcc gatatgatgt gggaaaatga acatttacat
    15061 tatgtgatcc caccagaagg atccaatctt tggtttgata atatcgttat gccaaaaacc
    15121 gtgaaaaata aagaaggtgc ctatgatttc atcaacttta tgctagaacc ggaaaatgcg
    15181 gcacaaaatg cagagtatat tggttattct acaccaaatg aagaagcaaa agcgttattg
    15241 ccaaaagaca tttcgtcgga cgaacaattc tatccatctg atgacacgat ctcccatctt
    15301 gaagtctaca aagatctagg atcgaaatac ctaggcatct ataatgacct attcttagaa
    15361 ttcaagatgt ataggagata atggcaaaac tcagtaagaa tggggactta tcaacgttct
    15421 aaagagctaa aaatcgtcct ttttctatct gagttttcca atcttctacc aaaagcggat
    15481 gaaacgccgc tttttttgtg ttattttatt ttttctacca cgtttttcta ccagagaaaa
    15541 gtggacaaaa ccattgtaaa atagggcccg agacgggcta gtaaatctaa gattgaaaaa
    15601 gttacagaag gataaagtaa ttggaaacta tgcaaatata cttaaccctt ctgtaatcta
    15661 ttaataaaac taatatatga atccagttca atatttgtag ctatgaaaaa acataaaaat
    15721 catattagat aaaaactaaa ttggtagata aattattttg tcagatatct gaggcaaata
    15781 ttcgactatt tgtgtttcaa agtaaaaatt ggaatacaaa tatagaatag acatattgca
    15841 gccgatagtt tattatgctt ttagacacat taaactggag gaaaaaatga aaaagagcgc
    15901 taaagatcga gtttttgatg agctgatacg actaacaggg aatatgtctt ctgtcactgc
    15961 cgagaaaatt tcgaagaatc taggtatctc tcgtcagaat gccagccatt atttgacgag
    16021 attagtggaa gataaaaagg tcgagaaatt accgggaaag cctgttttgt ggaagccttt
    16081 agatgaatat gcggttgtag ataatacgga gcaaataaac gaagcctttc attctgtagt
    16141 tggtcatgac ggaagtctga gagaggtcat tcaaaaatgt atagccgctg tcaaatatcc
    16201 gcctaacgga ctcagtgtat taataaatgg agccacgggt gttggaaaaa gttttttagc
    16261 cacaaaaatt tttgaatacg ctatccatga gcaaattatt gaaaaagatg caccatttgc
    16321 tatcttaaac tgtgccgatt atgcggacaa tcccgaactg ctttcggcaa cactgtttgg
    16381 atacaagaag ggggccttta cgggggctga aaaggatacc gatggtttat tggcaacggc
    16441 caacaacggc tatctttttc tcgatgaggt ccatcgccta tcgaaagaaa atcaagaaaa
    16501 gctctttcta tttattgata ctggaaacta tcgtccagtc ggtgaaaatg taaattggca
    16561 ttctgctaag gttcgtttta ttttcgccac gacggaaaaa ggtgaaaatt atttattaga
    16621 cacctttgat cggcgtattc aaattagtgt tatgttgcca acttttgaag atcgccccat
    16681 tcgagaacga ttagagctga ttcaattatt ttttcaaaat gaagcggatg ttttacaaaa
    16741 agatattatt gtttcaagag aagccttgtc tttaatgtta tctcatccct ttagtgggaa
    16801 tattggtaag ctgaagaata tcattaaaat cagttgtgcg gatgtgtata gtcgaacaca
    16861 agaagaacca ttaacgattg ggaaaaatga gatcttgatt cagttaaata tgctggaatc
    16921 cgaagtagaa agcttgccga ttgctagttt attgataaag aaaaatcaaa agataaaaac
    16981 ggatcgaatc aaaccgtttg attatgattc gattttggaa aaaattactc agctgataaa
    17041 tgaaggtaat ttcaatgaaa atttttcttc gataaagaat gaagtacagc atttggtgca
    17101 tcacattgat aaggattctt ttttatcaat gagtagaaaa ttgatgattc agatgtttag
    17161 aaaggtctgg gtatccgttt taggaaaaac gtacggattg acgacgagtg gacctgttgc
    17221 agaaattatt agcagatcct atgtcatttc cccaagctat aagacagact taaccgggat
    17281 aaatcgtaca ttagctgcaa atctacctcg gacgacttac ttatgtaatc agttcttgga
    17341 gcatttacca ccgctctcag aaaaggacga gaactttcta agactgctta ttgtcgtgac
    17401 tttatctgat tatgtagatg aatccatcga gctgaagggc ctgttgcttg ctcacggaga
    17461 tagtacggct agcagcattc aagcagtagt gaatcaatta tgcggcaatt atgtttttga
    17521 agcgttggat atgccagtag agagtaatgt gtcagagatt gtcgaatcga caaggaattt
    17581 cgtgaaacgg caagtgaata cagatagtct agtgctgatc gtagatatgg gctctttaaa
    17641 tcaaatttac acacagatca agaacgattt aaaaggggag ttacttcttc tgaataattt
    17701 gacgacttcc attgcacttg atattggctt gaagatgacg atgaatgcgc catttaaaca
    17761 gattgcggag gatgcagcag acaagtattc aatcaatgtt caatactttg aaggcttttc
    17821 tcaaagtagc aacattatca tttcctgtat gtcgggtttg gggatttcgg acaaattgaa
    17881 agatgttttt cgtcatcatt tatcggatga atccatcgaa gttttcacga aggactacag
    17941 agaactacgt gaattaatcg accaaaatga tgaaacttat tttgacaaga caaaattggt
    18001 catcacgact tcggatcttc cacgttcctt ttcaattcct aatattaatg tttatgacat
    18061 cttagacggt gatggagcag ccaatttgga gcatattttg agtcctgaaa tgaagccggc
    18121 agaatttaac cgcttgattc aggagctagt aaaattcttt tcgttggaag gaattgcgga
    18181 ccgtttgaac tttctgaacc caacgatcgt gatgagtgaa gtggaaacag tcatttctaa
    18241 atatgaaaat tactatcatt tcactttaaa tggccgtgta aaactaaatc tctatatgca
    18301 tacggcattg atgatggaac gtttattctt ggctcggaca gaaaaatctg aagaaccgaa
    18361 ttatgatttg aaaccagatg aagatgaatt ttattatgtt tctagaagta tttttcaatc
    18421 aatggagatg aaatataatt ttaaagtcaa tgcttatgag ctttcgctgc tctatgaatt
    18481 attaggaccg tatattcaaa aataaagtgt ctaaaaaagt gtctaatctg ttttttttag
    18541 gttagacact ttttttaatg tgaattaacg tgattaaacc aatgttttta aaagttggca
    18601 caggaattgc ttatataaaa gtgaacagaa agaaaggaat taaccatatg aagaaaattc
    18661 taatagcgac tcacggtcac ttagcttcag gctttcttag cagtattcaa ttactgacgg
    18721 ggaaggttca agagattacg gtgattaatg cttatgtgga tgagtgtgat tttgaacagg
    18781 agttgaatgt atttgtaaac caggtgaacg agcaggatca gatttttgtt tttaccgatt
    18841 tatttggcgg aagtgtcaat cagaagatta ccaaaacctt tttggagaaa gagattgctg
    18901 taactatttt gtctggcttt aatctgccaa ttctgctcga aatagtattg gcaaccaagg
    18961 aattaacaaa ggaggatatt caggaactgg taaataaatg ccaacaggaa ttaaagatga
    19021 ccattcttga tgaggtacat gtagtaaatg aagaggaaga ttttttttag gaggaagaaa
    19081 aatgattaaa gcagtgcgaa ttgatgaacg attaattcat ggacaagtag cgatgacttg
    19141 gacaaagagt ctaaatttaa ccgggttagt agtagcaagc gatgaggcgg ctaccaacga
    19201 tattcaaaag atgacattga agatggctgc accatcgaat gtcaaagtca ttattaaaac
    19261 agttgatgac gcgattcatt tgttaaaaga tccgcgggcg aaggacatgc gcttgatggt
    19321 tttagtccct aatgtcaaag atgcggtaaa agttgcgaaa gctttcccaa cggaaatcga
    19381 attaatgaac gtggggaatg ctgggaaaat gacaccgcca tcggaagata agaaaacctt
    19441 atcaaaggaa gtgatgttaa cgacgtcaga aattcaagac ttaaaagaat tagttgaact
    19501 ttatccagat accgtttttc agcctacacc ggcgatggaa aaacgttcag ctatctctat
    19561 tttgagtgat ttttagaaaa aggaaggaga aaaattatgt atgatgcatt tgtagtcgct
    19621 ttggccgttt ttattggagt tgctggacat gaaatgttcg ggatgtccat gctgagtcgt
    19681 ccgattgttg ttgctccgtt agcaggagcg ttgatggggg atttgcagac ggggttgatt
    19741 gtcggggcgt cactagaagc catctttatg ggtgtcgtca atatcggaat ctctagtacg
    19801 gcggagcctt ctttggctgc tgggctggct acagcgttcg caatcaaaat gggaggaaac
    19861 gtggatgcga ttattcccat tgtctttcca ctagcagtat taggattaca gttaatgaat
    19921 atgatttttt cttttgtatg tggaccgatg gctgctaaat ttttcaaatt tgcaaaatct
    19981 ggtgatcaaa aaggaatcat caatctgcac ttctttatgt ggtttgtcca ctatgcgctt
    20041 tatgctctta ttccattctt tgccgtttta tttggagcag atgttgtcaa aagcgtcttg
    20101 gataatattc cagaggtcat catgaacgga ttaacagtcg ctggaaatct gctgccggca
    20161 gttgggatgg cgatgctgtt acggatgctt tggagtaaaa atattgctgt gtggtttttc
    20221 tttggggccg ttgttatggc gtatctcgag ttaccattga ttgcaattgc cgcagttgga
    20281 acgattattg caattgttat agcccaaaga gatctacaac taaaaaatat atctgttcat
    20341 gcagttcaaa gcgatgctgc tgtaaatgca acgcttgacc aagaagagga ggatttcttt
    20401 caatgaaatt agctgatgga atttctaaag atgagaaaaa attggttcgg aagatgtttt
    20461 ggcgctcgat gactatgtat gcttcggtga atccggtgac gatgggcggc ggtggtttcg
    20521 cctattctat gatgcccttc attaatcatt tctacaaaag tgaagaagat cgagctgcgg
    20581 cgctggaacg tcatacacag tatttttcga cgaccattcc ttgcgcgtcc tttgtcatgg
    20641 ggattgcggc atcgatggag aaggaaaatt ctgaaaaagc tggttttgac gtggaaagta
    20701 tcaacgcgat caaaacgagt ttgatgggac cattggctgg aattggcgat tcattgttct
    20761 ggggagtttg gcgtgtcgtt accgcgggac tagcgatcaa cttagccaat gccggaaata
    20821 ttttggcacc aattatcttt ttagttatgt tcaatatacc taattacttg atgcgatact
    20881 atggcggcgt tttaggctat tcattaggct ctaaatatat tgaaaagctg tatgcgaacg
    20941 ggttaattga aattctgaca aaagctgcta gtatcttggg tctgatcatg gtaggtgcaa
    21001 tgacgatcag tatggtcaaa ttcaataccg ttttgagctt ttcaatgaag ggcgaagaac
    21061 taatgaatgt ccaagaaatc ctcgatagta tttttaaagg aattgtaccg ttattggtaa
    21121 cattgagctg tttcaaatta ctagataaaa aagtcggcgt catcaaaatt atttttggaa
    21181 tggtcgtctt gggaatttta ttgtcattac ttggaatcgc atagtaggaa gctgcagcta
    21241 ctaggctgca gtttttttag caaggaggga aaagatgact attaatgaag taatttattc
    21301 acccaaacac ccatttttta atgctgagtt aaacgacgga aatgtgacta cttcaaacga
    21361 atacagctac tatcggaata ttcctgatgt aaagggattg accttcgatg taagtaatca
    21421 aaagacatta gcaaaccttg atgtttgcgg gacgattcaa acgttgacct tttatcgaga
    21481 aaatttacta actgaggaaa aaccaggtgt ttgggtcaat aaacagctct ctcaaaccaa
    21541 ttcgttatcc ttaaaggttg aagtaaacgg gattgtctac catttgtcag aatcggatca
    21601 tcgtattgaa gtcgatgtga ttcaagactg tttgcctaga tatattcatc attattcgac
    21661 gtttaaggtc attgtagtat cctttgctcc catactggct aataagcgtt taagtatgct
    21721 tgttcaacag gtttacattg tgaatgaaac acacgaaagc cttcaagttt cagtgaaaga
    21781 tgtgccttcg tatcaagaga agtattcgga tcagcaaaat gtgttgattt ctcaaaaagg
    21841 aaataaggac acgatcgcac aacaggaagt ggcaagcttc gccattgctt ttgtggaccc
    21901 aaatgctttt gaagaagtaa tgacttttca ggaatctgat atcgaaaaat ggctgaagga
    21961 aacattgaac tattttgctc agctatttgg gcagctgtca atgccaaacg atagagttgt
    22021 ccatctctat cgtcgtgcac tttatcaaag cttttctagt ttcggtatga atcgacaggg
    22081 acaggtcgtc ggcagcaatt ggggcagcta tcctgctact aatcgaattt ggaataagga
    22141 tatgtattac gcatcattgc cattcaccat gtttgaaccg gagttatgtc aaaaaacggt
    22201 tctatggttt gatcaatatg gcgttaagtt tcctggttca aaatttacag gaggtatcaa
    22261 tcattctctg agtaattcgt tatccagtgc gttactggct agtctttatt ttgaacatac
    22321 tggggataca aaattctttg agcggtgtcc gcaggttcta ctgaatgctt caagaattat
    22381 tgaggacata ttagctcaac gtcggtcagg ggaaccaatg ctttttccat ctgtttggtt
    22441 atcagacgcc tttgcacttg gaaagtatca taccggcagc aatctttgtc tttggaaggc
    22501 ctgtattgga cttgcagaaa tttttgaagc cctagaacaa ctgagtctta gcaagcgata
    22561 caaaaacatt gcctgtaaac taaaggaatc aatttgtaaa caaatgacta tcgatggtcc
    22621 ttttggtgag caatttttgg agggaatcgg tgatgaggag aaaacaacct attcagtgaa
    22681 gcactatcaa aaaccgattc ttgagcaagg gttaatcttt ttaagtgatg tgattgtgga
    22741 tggaaaaatt aatttattga tgcatgatgg agaagaatca gacactacgt tgatgccttt
    22801 ttatcaattt ttagataata aagatcattt atatcagaac accatggcct tttctgctag
    22861 ttctttcaat ccaacctata gcgaggaaat caaaggcatc acttggggat tagaatccgg
    22921 cgccactttt cctggcttta tcactgtttt aatgagcgag ctgcacaacc ctcaagcttt
    22981 cgtaacccga ttagaagaac taatttgttt ggcagattta gacggttcgt ggtggtggtg
    23041 gccgtatcgc ttagaggctc aacaaggaga agtcgtgcga gactttggct gcggcaaatg
    23101 cggctgggca tcgggtcttt ttgtcagcct aatgatcagt caatatttcg gattgaaatt
    23161 caaaaaagga gtattgcaaa ttgatcctgt tgataattta acgttttctt ggaagaattt
    23221 gaagttcggt caagccttca tttcgattga atgtacgcaa tccgagttga gtatttcaaa
    23281 tttgtcagaa aagccattga aaataagtga tacgaaaaaa attcttcacg tagaaaaggg
    23341 aaaaacgatt cacattcaaa ggagaaaaag atgagtatac taaaaaatat tacccaattt
    23401 aaaaaaatcc agagtcgagc aatcactccc gaaaatccaa caggcgagaa aggtaaagct
    23461 gcgatgttgc caagcgagct aggaccaagc cgcaaaggtc gtggcgcaat tccgctgact
    23521 tctggtgaag aaactgtgat tgcggatatt actggaacgg gaataattcg tcatatgtgg
    23581 atgacgattc gtgatcgaac agaatttggc agttttgttt tacgtgattt aatcttaaga
    23641 atttattggg atgggtcgga tagccctgct gttgaaagtc ctttagggga tttcttttgt
    23701 aatgggtttg gagagcgttg cgaggtgaac tcgttgccga tagtggttaa tcctacaggg
    23761 ggctttaatt cctatttcga aatgcctttc caaacatccg ctaaaattac gatcgagaat
    23821 ttacatccga aggatatgtc ctcatttttt tatacgataa attacgcttt ggtggacgaa
    23881 ttggaagaaa atagcctata ttttcacgca aattggcatc gggaacgcat taccaaatta
    23941 caggaggatt ataagttaat tgatcgatta aagggtcaag gctattacgt gggtacttac
    24001 cttgcgctta cctgcctaga acgttattgg tggggcgaag gcgagtttaa attctacatt
    24061 gatggggata gtgagtatcc gaccgtaacc tcaacaggtt cagaagatta ttttggcggg
    24121 gcgtgggcct ttcatgagta cgatgagttc ggacgtccac atgcgaaaaa ctattcaaca
    24181 gcctttttag ggtatcctta ccaatcaaat agagatgcca caagagatcg ttttgaaacg
    24241 ggaaaattga atgccgttca tgcctttggt gacgacgcaa tgccacgaca tggtctttat
    24301 cgttggcatc ttcaagaccc catctgtttt accgaagatt tatcggtcac tttacaacaa
    24361 atcggcaacg atgatgttcg cttatttgag cggcaagacg atgttgctag tgtcgcttac
    24421 tggtattcaa cagaagcttc aggaaatact aaacgtcatt tcactgatcg agaacgcttg
    24481 ccgcgttaat gaagtgtttt tcacatattc gaagggagga acctatgatg atatatgggg
    24541 caattgaagc tggagggact aaatttgtct gtgcagtcgg tactgaggaa ttaacgattt
    24601 tggaaagagt cagttttccc acaacaactc cagttgaaac aatgaagcag gtcattgatt
    24661 tttttaaacc ttttaaggaa caattggcag gcatcggggt cggctgcttt ggtccaattg
    24721 atattcaatg caaatcaacg agtttcggtc atatcacatc aacaccaaaa cttgcttggc
    24781 aaaatttcaa ttttgtagga acgctaaagc aatatttcaa tattccgatt gaatggacaa
    24841 cagatgtcaa tgcggcttgc tatggtgaat atgttgctgg ttctggcaaa gggttagcag
    24901 gtgttgcata cttcactgtg ggaacaggta tcggaggtgg tgccttgttc aatgggactt
    24961 tcgtggaagg gttcagtcat ccggaaatgg ggcatatgtt gatcaagcga catccaaagg
    25021 acaatttttc aggaaactgt cccttccacc aagattgttt agaggggatg gcagcaggac
    25081 cagcgattga aaaacggttg ggtgtaaaag gccagaattt attggctgac gattcgtttt
    25141 ggcagattga agccttttac cttgcccaat gtgcatacaa tacaacactg atgttttcgc
    25201 cagatagaat tatttttggc ggtggtgtaa tgaaacaaga acacatgaag aaaaaagttc
    25261 aggataaatt tgttgagctt ataaatggat atgttgaaat accaccaatt gatagctaca
    25321 ttattacgcc cgaattaggt gataatgccg gaataatcgg cggattagca ttggccagaa
    25381 aagcggtaag aaataagcaa ccataattaa tgaatgtttc attgatactc atgtcaaaag
    25441 tatttaaccg agtaatcaaa tgatcaaatg aagttggata aagcgaaaat gaattcaaat
    25501 aaaaaggctc taagtcaaat cgtgttgttt tcattaaatc actgaaagga aatgcttctg
    25561 cgtttccttt tttctttttg ttgtgcgctg tataccttga ggatattcac tggtggcgta
    25621 cacttaaata gtaatatatt gtgttacttt atgtctgtgg taatagttat agttgcttac
    25681 caatgtatgt tcatt
//
LOCUS       DAZUMM010000035        25270 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000035 DAZUMM010000000
VERSION     DAZUMM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.035"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(113..628)
                     /locus_tag="ACG8E6_001383"
     CDS             complement(113..628)
                     /locus_tag="ACG8E6_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HFS5061808.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNVERLSF"
     gene            complement(810..1421)
                     /locus_tag="ACG8E6_001384"
     CDS             complement(810..1421)
                     /locus_tag="ACG8E6_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS5061809.1"
                     /translation="MIDIISAITVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            complement(1447..2898)
                     /locus_tag="ACG8E6_001385"
     CDS             complement(1447..2898)
                     /locus_tag="ACG8E6_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5061810.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQA
                     VAYTTVFSYMFFCVIHYIFYRKVCKELLDGKNIYDVRGILLISAITIAGGILISIINK
                     FDFIKYLIIAIACVAAIIFRKRLLLLVRRIMSK"
     gene            complement(2922..3653)
                     /locus_tag="ACG8E6_001386"
     CDS             complement(2922..3653)
                     /locus_tag="ACG8E6_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5061811.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            complement(3671..4066)
                     /locus_tag="ACG8E6_001387"
     CDS             complement(3671..4066)
                     /locus_tag="ACG8E6_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFS5061812.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            complement(4168..5805)
                     /locus_tag="ACG8E6_001388"
     CDS             complement(4168..5805)
                     /locus_tag="ACG8E6_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFS5061813.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            complement(5840..7174)
                     /gene="gmhB"
                     /locus_tag="ACG8E6_001389"
     CDS             complement(5840..7174)
                     /gene="gmhB"
                     /locus_tag="ACG8E6_001389"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFS5061814.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            complement(7216..8325)
                     /locus_tag="ACG8E6_001390"
     CDS             complement(7216..8325)
                     /locus_tag="ACG8E6_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WavE lipopolysaccharide synthesis family
                     protein"
                     /protein_id="HFS5061815.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAEYEKYMDFVWEE"
     gene            complement(8350..9585)
                     /locus_tag="ACG8E6_001391"
     CDS             complement(8350..9585)
                     /locus_tag="ACG8E6_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061816.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFIPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLNVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            complement(9616..10722)
                     /locus_tag="ACG8E6_001392"
     CDS             complement(9616..10722)
                     /locus_tag="ACG8E6_001392"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS5061817.1"
                     /translation="MKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRDVPPLDPKI
                     KSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYEGGLYVLCK
                     RHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAEFPELNAKS
                     YVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMDVRKKVHFT
                     IAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAIFPYKEIYG
                     SGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKYVNLSPAEK
                     SKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            complement(10733..11506)
                     /locus_tag="ACG8E6_001393"
     CDS             complement(10733..11506)
                     /locus_tag="ACG8E6_001393"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HFS5061818.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            complement(11520..11975)
                     /locus_tag="ACG8E6_001394"
     CDS             complement(11520..11975)
                     /locus_tag="ACG8E6_001394"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFS5061819.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            complement(12397..13503)
                     /locus_tag="ACG8E6_001395"
     CDS             complement(12397..13503)
                     /locus_tag="ACG8E6_001395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS5061820.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRNVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            complement(13521..14282)
                     /locus_tag="ACG8E6_001396"
     CDS             complement(13521..14282)
                     /locus_tag="ACG8E6_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HFS5061821.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            complement(14358..15356)
                     /locus_tag="ACG8E6_001397"
     CDS             complement(14358..15356)
                     /locus_tag="ACG8E6_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS5061822.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWEPTVSLEEGLRESYNWIEKQIKG"
     gene            complement(15375..16028)
                     /locus_tag="ACG8E6_001398"
     CDS             complement(15375..16028)
                     /locus_tag="ACG8E6_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5061823.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            complement(16038..17048)
                     /locus_tag="ACG8E6_001399"
     CDS             complement(16038..17048)
                     /locus_tag="ACG8E6_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HFS5061824.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFENGGTRVIHYTPETYIEKQ
                     NG"
     gene            complement(17067..18317)
                     /locus_tag="ACG8E6_001400"
     CDS             complement(17067..18317)
                     /locus_tag="ACG8E6_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFS5061825.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            complement(18824..19104)
                     /locus_tag="ACG8E6_001401"
                     /pseudo
     CDS             complement(18824..19104)
                     /locus_tag="ACG8E6_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697748.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(19663..19773)
                     /locus_tag="ACG8E6_001402"
     CDS             complement(19663..19773)
                     /locus_tag="ACG8E6_001402"
                     /inference="COORDINATES: protein motif:HMM:NF020874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HFS5061826.1"
                     /translation="MNPNGYEWLRAKGSYPVPKYCNYSETLMEWYQLIIT"
     gene            complement(20179..20868)
                     /locus_tag="ACG8E6_001403"
     CDS             complement(20179..20868)
                     /locus_tag="ACG8E6_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS5061827.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(20913..21677)
                     /locus_tag="ACG8E6_001404"
     CDS             complement(20913..21677)
                     /locus_tag="ACG8E6_001404"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFS5061828.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(21705..22403)
                     /locus_tag="ACG8E6_001405"
     CDS             complement(21705..22403)
                     /locus_tag="ACG8E6_001405"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFS5061829.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(22416..23195)
                     /locus_tag="ACG8E6_001406"
     CDS             complement(22416..23195)
                     /locus_tag="ACG8E6_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFS5061830.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            complement(23211..24155)
                     /locus_tag="ACG8E6_001407"
     CDS             complement(23211..24155)
                     /locus_tag="ACG8E6_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5061831.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(24196..24975)
                     /locus_tag="ACG8E6_001408"
     CDS             complement(24196..24975)
                     /locus_tag="ACG8E6_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061832.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHFNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
BASE COUNT         7786 a         5433 c         3700 g         8351 t
ORIGIN      
        1 atctatatct aaatttagtt ttatgtattc ctcatagaaa taaccgagca tttgctcggt
       61 tattgttctt cttattttcc agtatccttc ttttctccac agctcatttt aatcaaaagc
      121 ttaatctttc cacatttttg tctttctcac ttataacgaa acactgccga ctgaccggcc
      181 attcaatatc tacgctttca taacttatac atttggcagc attagaattg aaatcattat
      241 ctacgcacca ctgaactagc gtattgtcct ccatagtaag atagccatga gcatatccac
      301 gtgggatata cgcacattta ccattgcttt cactcaatgt gttgacatac acacatccat
      361 aagtggaaga tccttgtcga atatcaagtg cgatattcat aatttcacca tgtaggcaca
      421 tcactatctt tccttgagca tgctctccac tctgataatg tagccctcgc atagtatatt
      481 tattatgact aaatccttga tttatttgct taatgtgaaa ctcaatacca atcgagaaaa
      541 gagtctcatt atccaatgca acagcagtat atcctctgaa atccgaaaac ttaattatat
      601 caaacacttt taccataact accaccatgt tatatatttt accaattttt taattcgctc
      661 gacctcattt aaagcaataa aattccctac ctcttgaaga tataacaaca atgttttagc
      721 aaaaatagat ggcatgtaaa gtattcttga tgagattaca caatcctgtc tgcagaaaaa
      781 tattaaccat attaaaattc aaagcagttc tattttaagt ctatatactt atttaatcgg
      841 tagaatagcc aatatagcaa agcaccaatt aagcccccta gggtgttata aaacaaatcc
      901 gatagttgaa atgtaccaag cctaaaaaac agttgtgtaa attctatact taatgagaat
      961 aaaaacacat acttaatccc aacatagatg gtgcgtataa tatcacattt aatgtcacgc
     1021 caccataaca ataacatgca aaaagggata aacagcatcg tgttctccac gcattctgtt
     1081 gtcaatctgc tctctcctgt agcattatct ataatatata atgaccactg tccaataacg
     1141 tttgaaagcg ggttcatcca catatttcga ttaagcaagg ttttaaataa tatcaaagta
     1201 aaataaaacg caagaaaaaa gagtctgcga aatttactag atgctttaaa atatctcaac
     1261 cagattaata cagaatcttt taagttttta ggatctcgag agttatcaac tataaataga
     1321 taccagtaca taaacagata cgctgttaat acggcaaata agaacggctg atataatgct
     1381 gttaatacat ttaccgttat agcagaaata atatctatca ttctctatct ctctatgagc
     1441 aaatgtttat ttgctcataa ttctccttac taaaagtagc aatcgttttc taaatataat
     1501 tgcagcgaca caagcaattg ctataattaa atattttatg aaatcaaatt tattaattat
     1561 tgaaattagg atgccaccag ctatcgtgat tgcagaaatc aaaagtattc ctctgacatc
     1621 atatatgttt tttccatcca atagttcttt gcaaactttt ctataaaaaa tataatggat
     1681 gacgcagaaa aacatatatg agaatacagt tgtgtatgct accgcctgat acccatataa
     1741 ctttataaaa acataattta gtacaatatt cataattgca cacaaaaccg atggaataac
     1801 gacagtgagt ttcttttcaa agtattcctg tactctagca aacagctgaa acatatagtt
     1861 gaaataaata ccgatacata taggtacaat taaattaaca ctgtcgatat atttctgccc
     1921 accaaatatc aacacaattt ctttactaaa tagcattaat gcaaaaagca tagccgctac
     1981 caaaccaaga acttcattag ctctttttga tagtaaatca tattctcctg acttgatttt
     2041 ttgatactga taaggtgcaa aagtggaatt gacagaattt gtaacaaggt taattagagc
     2101 accaatagaa tatgccacgc tgtaaatagc aacatctgac gaaccacata agtaatttat
     2161 cataatttta tcagaaccat tcagaataaa ttcagataaa tagtgtggca tcaacgatac
     2221 acaaaatact actgcgaaat tccacacttc attatcctta aaatccttac atcttctaaa
     2281 taccgatata tacacagcca cgccaacaac accagtcact aggacattca ttaaggctct
     2341 cgcctcagcg gtaggagata taaacagaac cgctaaacag ccaactacag atgagccaac
     2401 agtaactgca agggtagcta ctaataaagt cttataatca tatatatatc gtttgtgtaa
     2461 agaccaacat tgtatttctg cctgaaatat aaaatatgag aacatacaga caactagaac
     2521 tggtgtcaat cctaaaattt ctgccaatat atccttaata aaaatagcaa taacaaacca
     2581 aaaaatagtc agtactaaag tcaatccgga tactgtcgaa agaatatgtt cttgattatc
     2641 atattttaca taaagattca tcatggcttt gaacaaacta agcgatgtaa tcatgagcaa
     2701 taaggattcc cacgatagat aagtgctgta taaaccgtat tgttctgtag acaaaactct
     2761 agtaaaaatt ggggttgtaa tcattgaaac ccctctctgt aaaaaaccac ataccataaa
     2821 ccataatgca gccttaacag gaagtggagt attcttatat ctgcttaatg tttttacaat
     2881 tagattatta ctggtcactg ctattttctc ctaaacacta tttattcttg attcaaaaac
     2941 acattgagct cttctggagt accaagcaca tgaacctttt tcgcatctac tatgctaata
     3001 gtcaccttta accctttttt tatcataaag ttatataatg gtgctatata tttttcattc
     3061 ttctccatat tagaggagtc tgcataatac tcttcatata aagctttata cagctttgcc
     3121 gatgagaaat aatacgcacc aagagtacaa ttatcagaaa tgcgaacttt ttcgcggact
     3181 tctattactt catttcgatc gttaagcttt gcaaaactcc aatgatcgcc atcagcatga
     3241 aagcatggaa tatgtccatc tccacaaata tcttcgtatt tcatctcatt tgattccacg
     3301 tatgtatcaa tattgtaaac cataatctca ttgtcttgat tgcaataagg aatagccaac
     3361 atacatgtag ttgcttgacc atctgtcata tagtctattt caatcacttc tacgttttca
     3421 ataccaaagt aagcacaatg ctcctttata aaagctgaag cgttgtcttc ctgacgaaca
     3481 acaaaaatat acttatcaac atttttattg tatccaacca aactatccat ggcccaatca
     3541 aataaagttc ttcctttggc attaatcata tatttaggaa ccgcataacc aatttttcga
     3601 aaacgacttc ccaaacctgc catcgtaatg ataactgtca tctttttttc catagaacta
     3661 acctccttat ttagaatctt ttttttgaac tgttgcagat ttcatttaat tcatctacag
     3721 agcatctcaa aaactcatca ggtctaaccg ttcgatcatc tacatagaag cctttatgcc
     3781 ccggccaagg cttaccatac aggatttcat catacggaat gtcccattta tccaaccacg
     3841 ccaagagcac ttttgcagta ttagcattta tcattcccaa atttccttta taggaattaa
     3901 cattgcgtga ggtgaacaat gtaattttag caccattctc cttatagaat ctcagttttt
     3961 caaccatatt ctcatacggc accaaatcct catatcgctc atccttcttc tttatcggac
     4021 acaaagtgcc atcgatatca aacacaaaag aatattcact atacattttt ctcttccctt
     4081 ctattgtatt aattaatata atcgtgcctt aatgcctaca ttgctaaaac agtattgtaa
     4141 ccgttattca ttaaggcaca atttatatta gagtataact ttattactta ctcttgcaag
     4201 aagttccata cctgtagcaa gcatcactat ttggtgatca aaactttcac cgtgtaatgg
     4261 aatcatacta agaaatagca acgcttctat caatacaatc ttatcaaaat cgtccttaat
     4321 ctcgtccgaa aagaccgact taaacaattc aaataaatcg taatctctta cacgcgtatt
     4381 aattgtataa tctatcactg cacactcggt atcatattct acagtaaata gatccttgat
     4441 gatataatca tatttaccat ctaaactatg aaatagtttt gctaattcat atctgctgtc
     4501 cccataaata tcatattttc caaattttcc cctaggatca ataaccttaa caaaggaaca
     4561 attggaatct accatcatat tagcaaaaca caaatctcca tgtataatac aaaattcatt
     4621 tacatcatag agcatacttg ggataagatt ctttagaagt tcaattacac cggttaaaga
     4681 aataaactta cgaccattaa tttgaatttg tttctcaaaa aaagctgaaa atcttttatc
     4741 ttcagacaat ttttgaaatc tctgcatggt ttttcgcaaa tacatatctt ccaatgactc
     4801 tctaatcttt tcatctttta aggtgtatcg cttaaaatcg ctacagataa actttatacg
     4861 gctaaacaga tcatgccact ggttatattt taaatcacca tataagtata actcatgaac
     4921 cgtatgatat gcataatatt ccatatcaac atagggagct acgtaagatg tagaataatc
     4981 aaaaattcta ggtcttacat attcaatatc agcaggaagt tttaaatacc actttatttc
     5041 tccaataaat ttatccttgt catcgctagt tttcttcaat atccctcgat ctttatctat
     5101 agagatatga ttaaactctc gtgctttaac ttccaacttt gtattgtaat atttatcgat
     5161 gtgtccaata tcaaaccact tttctgttct tacggccttt agcggacaac gttcgctgta
     5221 tagctgtaaa gcccgataaa atgagtttat tcccccctgt ttccgactct caactgcctc
     5281 tcttaagcaa ctcttcagaa gttttgacga agaaaacata aatacgccaa taaataattt
     5341 ttcacgttta tcggatacgt aagcttcctc tttatcgtgg atatcagtaa ttattccttc
     5401 attaatttta aagtatgtcc aagtactcga cacaaaatct tcagaataat agaatgtatc
     5461 catttctata tcagacatac tctccataac aattgtatct gcgaagttaa ttagaacagg
     5521 ctcatcttca tctgataacc caaaatacac ggtttgccct agatccccta attcaggaag
     5581 gtcacatata tgaattctgg aatcatgata gtttttcagt cttctatgaa ccttatccgc
     5641 tttttcataa cagataatat ccaatgaatc gcagattgca ttatactgat tatacaaata
     5701 atcaaagaca atttttttat taattggata gataattgct ggtaacattc ctatactctg
     5761 caattctttt tcaacaactt ttgctgctgg aattaatacc ttcatctaat tactcctttt
     5821 acaagtttca ctaaaccaat tattgcatag tgcataaatg atttttaaca aattctaaca
     5881 atgaacagac actatagtct tggccataat tagcaggcaa ggtgatgtct tctgtaatca
     5941 gtactgtctt acaaccagca ttttttccag caattacatc actttcacta tctccaacca
     6001 taaatgattg acttaggttt atgttaaaat cctttgatgc ttctaataac agtccaggtt
     6061 ttggcttgcg acaattgcaa tcaattttta attctataac ctctcctgca tatcctttat
     6121 ggggatggtg cggacagtaa taaataccgt ttaaaaatcc accagcatgc cctagcattg
     6181 tttcaagctt attgtgaata ctattaagct catcaacagt aacttcacct cttgcaatta
     6241 caggctgatt tgttataaca atacacagaa aacctagaga attaataatt ttaattgcct
     6301 ccgatactcc atcaattaac tcaaattctt ctgcatttct caaaaaacca acgtatttat
     6361 taatcgttcc atctctatct aaaaagattg ctttttgtgg attggataga tttttaccaa
     6421 caataattcc cttttgaaag tccttaatta cagctctata tcggtccggt gttcccatat
     6481 ctttaacata ctctggagaa tcgtagcaat acatacgccc tgtgttagcc aatggtttca
     6541 gaatcattct atctaaatca attttgtatg gtagcccccc accttcaact attgatttat
     6601 ttacaatttc cattaatgga ttttctctca acaccttcgg atttataaca tgaagacctg
     6661 catttactcg gtttttataa tattcaggtc tttgatcttc cttcgctaac cattgaagca
     6721 ctttcttatt gtcatcggat ataattaatc cactatcata cggatgtgag ttaggatgcg
     6781 taaacaaagt aacatctgcg ttattctcaa catgaaatct aatcatctgc ctgaaatcaa
     6841 catcaaatac tgcatcagca ttcaaaagta aaaacggttc atcacccagt tcatccaaca
     6901 aaaaatacaa tgcacctgca ttgccaagtg gagttttttc aataaaatat ttgattttaa
     6961 ttcctaattt tgaaccatct ccgaaatgct cctctatttt ttcatgcaaa taggaaacag
     7021 tcaaaataat atcattaaag ccttgatctc taagcgaaca caattctcgt tctaaaactg
     7081 gaatgccatc tataggaatc attggcttag gaatatccgg aaacaattcc gaaattcggc
     7141 ttcctcgtcc gcccgccata attactgttt tcataatatg cccttcccta aagtactttt
     7201 tactataaaa gttaattact cttcccaaac aaagtccata tatttttcat attctgcttt
     7261 gtattcgtat tttccagtgt acaaattcaa ccaatttata aaatcaaaat tatagcagtg
     7321 aagtgcatct tggctatcat caatataata cgtaccataa atagtattat ttgaataacg
     7381 acctacatat ttatgccagt acaaatcaat ttgatttttg ttaatagtaa taacatggtt
     7441 ttttacaaac tcccaatatg ccctaataga gcactcctca ttgcccccca ttcgtgaaat
     7501 atagcttcga agaagctgaa cttctggagc taaattattt tcagatatct cccttcttga
     7561 gactcctcta gacacagctc ccttttctct cacatcacta ggaatcgaga agaggtttat
     7621 catatcatca acatcaccaa aatacaggaa gtctgacaaa caatacggga aaaacatgtc
     7681 tccatacggc attgatagta acagtaatcg ctgattttgt attaatttcc aatcatcttc
     7741 gttattaaca ggatactgtt caagtaaact gcagaaaaac gccattgcat ttggatggta
     7801 tattctttga tctgttctag ttttacaaac atactttgca ccaagctgct ttgctctgac
     7861 tattccaggc aaagtgtttc caacttgata attaatattt aagtgtccag gcttttcagg
     7921 tttctcacta caaataacta taactccaag atcctctatc cttttcaatg ccttaatatc
     7981 ttcgtccttc catgtcgaaa gaataattaa agcatttgga taacatttct tatagtaggc
     8041 cagagtatta taagagaaat catcctttaa acatagtggc ccctgcatta caattgctac
     8101 atctttgcaa caattaacta aacccatcac tggaacatca gacatgtcct ttgagcgcaa
     8161 ttgaaacgct gcataaactt cagaattttc ttctactgtt gcacaatcag cagtcaattt
     8221 ccaagatacg agtttactta tattatgacc aaacatgttc ctaaacacat atggtaaatt
     8281 ttcgtacttc ttcaatatgt tatataaaat ctctttctta ttcatgagat gcactcctat
     8341 taatatttcc tattctatgt tagccacact tctacattct tgatagatgt attctataac
     8401 cgctaagcat acaggaatag ctggagtaaa tatcggataa aaccagtcta tattaatccc
     8461 tgttagcaag acatttaatg taattccaaa tgctaaatat tgatagccag tcatacccac
     8521 ttttgatgca agtaatgctg ttttgaaccc ttttataata aacagaatat caaatataag
     8581 tatacctaca ccatattgag taattgtata gaaaaatcca atatcagata ggtgtcctct
     8641 aactcctcct ggtaatataa acgtaaattc ctcaccagta agaataccag taccgaacaa
     8701 aggcgattgc aaaaatttag gaataaaaaa ccaaactgaa gcaaatcgag attcagcaga
     8761 tcctgcatat tcacctgtac tgtttgtgaa agtttcaaca aatgttgaga acatggtcga
     8821 attaataaat atgactacaa gcaacccagc taaaacccac attgcaattt ttcttttcga
     8881 agaacctttc ttaaaaagga tatagcaacc aagtacaata atttgataga caaggacaga
     8941 tctagaacca tgaataacag cagaatatgc cagtgcgcta gcaatcagta aataatattt
     9001 tacttttctt ctagctacaa cttgtcgctc aatcatatac aaagcaatcg gtattattat
     9061 aattgtaaaa catggtggat taattcttaa aaagcctcca cgataccaat tttccggggc
     9121 acactcacga tatatgtttt catagagaag tactcctgaa aatttctgaa taagtgaaat
     9181 agatgttcta agaatgtatg aaagaaatgt tacaccacat atcgtattta gcattgaatc
     9241 caaattccac gcttggttaa ttaaagtaca gaacaatgga tacgccaata atacatagta
     9301 taaactcata ctttccctag atttaaccca tattgtctta tctgagtaga tgtaagctgc
     9361 tattataaca acacaaatca gtaaagtgca gaaatttaaa tatctatttt ttctaaatgc
     9421 tggtaatgag gctattaaca aacatactat tgctagaatg actgtcacaa gtctaacgtt
     9481 catcgtttct cccaaccagc cgcccaaagg cttcaatatg cttgatgaga taatcattag
     9541 aaagaccatt agaaactgta tgattttcca tttactaatc tgcatgttaa ttattattcc
     9601 tttctaattg ttttactact cattcaaaat ttgtttgtat gcctcgtatg tgagttttgc
     9661 agatgccgcc caggtatact tgcggtttac aagctcatat atatcatcct ctattttgct
     9721 tttctcagct gggctaaggt tcacatactt tacaattgtg tctgctaggc tcttagaatt
     9781 caaattttcg aaaagtaacc ctatctttcc ttcctcggtt tcttccataa aagcaggtac
     9841 attactaaca atagttggta agcaataagt gtatgccata agaagcgctc cacttccata
     9901 gatttcttta tatggaaata ttgcggcatc tgcatttctg aacatactgt ccatttcttc
     9961 atcagaaatt ctctctggct tatatagaac acaatctgaa acctttaaat cttcaatcat
    10021 ttttttatag tcagtactat cctgtttagg atgttgttga cctgctatgg taaaatgaac
    10081 tttctttctt acatccattg gaagttctgt gattgcatta agcagtacat caattccctt
    10141 atatggacga atttgtccaa acaagataaa ttcaaactta tctttatcat acgctctaga
    10201 tatatgttta acatctccat agatagcatg tggacaaaca tatgactttg catttaattc
    10261 tgggaattca gcatataaat gctgttctgt tactcgattg tgaacaatga aaccatcaca
    10321 atttgaatat atattaaagt gctcttggcg atccttttca cgaacctcat gtggtaacac
    10381 attatgaact gtcataatta attttttata gtgtctctta cataaaacgt acaatccgcc
    10441 ttcatatttg agacttttca tcaattgcaa atgaacaata tcataatcac caaatcttat
    10501 ttccttccaa attaagaaca agcttttcag aaaaacaagt gccgaaaaca ctttatttgt
    10561 acttttatga tacaaaatat gtttgctctt aattttagga tctaaaggag gaacatcacg
    10621 cttacacagc aatgttaaat caacatattg attaagttct ttagaaagat caaacgaata
    10681 ccaattctct ggcatttggc tttcacacat caataccttc ataatactca ccttataacc
    10741 aatcaacaga aaattctgct ttcagttttc ctttaatttt acgttttgct ataaacattt
    10801 ccagtatctc tcgcattttc gtctgatatt ttgtcgtttt accataaagc gagtgattgt
    10861 cccctgttcc taatacatat actgctgctt tgaatggaag aggttttaat ggacgccctt
    10921 gctcttcaca tgctggaaca acgcccccat gtcgttttct aatgatccat ggattatcat
    10981 cacaatttgc tgtcataact tccggatatg cctcaggcag gtcttccact gcatatttta
    11041 ctatacacgc acttcctgat gagaactttg gtagaacctt catataatcg tctccaacaa
    11101 agtacacgta tcccgttttg acataaaatc cattttcctg gggatgggaa ttcacatatt
    11161 ccgccaaatg agaacttaca agatcatctg cgtcaaccat catagcgaaa ccgccgccca
    11221 actctcgcaa acgcattgcg agagcatgtg ttttataacc cttatcaatc attttttcgt
    11281 cttgatttgt gggaataggg gcatcctttt cgctaacttg aataaattca actctgtcat
    11341 catattcatg cgtgagttta ggaatttcat ggcaagcaac aatgattttg aaatcaggat
    11401 cagtctgatt ataacacgac cataatgttc ggttaaataa ggtactaacc atctcccaat
    11461 tgtttgctgc cttcgcactc attaaaggaa taacaaaata caacatacaa tctctccttt
    11521 tagtaacgat tcaaactaca ataatcattt acatcaatat cttttttcaa tactctagcc
    11581 ggaacgcctc ctaccgaaca attaggttca acatcattaa tgaccacagc attagcccct
    11641 actattacat tgtcccctat tgtgatatta cctaaaattt ttgcgccagt agcgatatat
    11701 acattgtttc caattgtagg cataccaggc ttattggatc taccaccagt cagtacattc
    11761 gttccgatta taacgtgatt acctattttt gtatcttttc caattacaca acctatgcca
    11821 ccgtataaga acttacaatt atctccaatt tctgtttgat atgaaattga gctattatga
    11881 agtacataaa ttagtgcctt tacaatcttg gcaaaaaact ccattttgtg taagtaaaac
    11941 catcgttcta ctttgtataa tttatatgca ttcatgtttt atctccccat ctttttagca
    12001 ggtacccccc acacttcgta gggtggaata gacctcaata ccactgtatt tgcagcaact
    12061 acagcacctt ccccaacgca taattctgtg ttagatttac aaagaatttt agcacccaca
    12121 cataaaacag cgttcttttt tactcgaatg atgatacgtt cattactatc tgctcaacca
    12181 cacgtcacat tctgatagac tttaacacca tcttcaagga ttgtgtcaga atgaatcaca
    12241 gtgcctacag aattatgaac aaattcaaca gagttcccta tctgtgcacc tttaccaata
    12301 caacaaccaa gaattttggc tatttttctc gtgcaattat caactatgtt taaagcatat
    12361 cgattcctca atgccattaa tttcgttact tccatatcat ttatcacctt ccaacatgct
    12421 aagcataatt tctcccgact ttgaccatct aaattctgta gttacacgct gatatgctgc
    12481 atctatgata tcgttggtaa atcgtccgtc ttttaagaat gtcaaaattt catcgtcaga
    12541 tttagccaac attccatatc tattatccaa aataatattc tgtgggccag gtgcattaac
    12601 agcaataatc ccgaggcgat agtacattgc ctctagcaaa accatacccc aaatttcagt
    12661 atagtttaga tttacaaata catccgataa gcaatataac tcccaaatat ctttgttagg
    12721 aatacgctca attattttta ctctattctg cagtccaagt aaatttatct gctgcttcgt
    12781 ttgctcgagt aaagcaccag ttcctaccat cagcaaataa ttatgctcat caagtttatt
    12841 aaataaacta atcatatcta acggtctttt atccttttca aaccgtccta catataaaat
    12901 aacacgaaca ttgcgaggaa tattccattt ttctctgaga gtagtaaaat ctattgcttc
    12961 gtaattctta tgtaactgtt caaaatccac acaaacagga gcaagtttca ctttatttac
    13021 tcctaattgt actaattttt cagaaactcg atcatttttg gcaacacacc catttttctt
    13081 ataaatttta taatttcgat gcgccaatat ttcaataatt gtctttatat atttattgcc
    13141 actttgactt cgcgttatcc cattataagg tatatatttt attccatttt ttacagtcca
    13201 tgagtataca tgaggtactg atagttgaag gtccgcaaat tgtattagaa catccgcatt
    13261 cttatcaaaa ttctttgttg ataagaatcc atttgatcct acataacgac acttgatata
    13321 gtgaatcatc gccgtattgc ttaattgctc tgttccaact tcctcatcac tttttacact
    13381 tttatataca ataacttcgt ggcctaattc agctaaccct cgagctaatc ctatttcctg
    13441 agaattatag gaccccttct taccaaagtt attcgtcatt gtaactacta taagtatttt
    13501 catctatttt tattgccccc ctacttagat tcaatagtat agtagatata ctttatacct
    13561 cccaagatat accgcctcca tagtctacga ggttccttta ccagtctaaa taaccactct
    13621 agactagctt tttccatcca ttcaggagct ttttccaaag tgcccgcaaa aaagttaaag
    13681 ccggcgccta caccgaacat tactgtgtta tggatcttct tctggtgttc ccacatccac
    13741 atttcttgct taggcgcacc aataccaacc cacagaaaat ctggatttaa gttgttgatt
    13801 tcctcgcaaa tcttgctatc ctcttcttca tttaacggac gaaatggtgg ggaatacatt
    13861 ccaacaattt tgattcccgg gtaatctctt tctaggtttg cacgaagctt atttaacact
    13921 tcgttatctt ttccaccata gaaataatgt gtttttccct gtctaacgcc ttcttcaaaa
    13981 accattccca taatatctgg tgcagcacat cgttcacagg taaatccttt gcgccgtcca
    14041 attttaacaa taggcatacc atctattgca gccattgtag cctggctata catttcagca
    14101 attttgggat tttcctttgc cgaaataatg gccggcactc ccacgagatc aacagtcgca
    14161 cttaccggag atgttggcat ggaaaaaact tcccttacaa actcgttctt tgtagtagca
    14221 caaacaccta ctattccaca cacagtgaaa tcacgttttc ttttatacat attcttttcc
    14281 atctcagtac atccttactc atatgttctt cctccgcatc ctctaattac acggaggaag
    14341 aacagcttat atacttatta acccttaatc tgcttttcaa tccaattgta ggattcacga
    14401 agaccctctt ccaaagaaac agtaggctcc caatccaaaa tctctttcgc cttagaataa
    14461 tctgcgcaac gagccttatc accttcaggc ttagaagtat caaagaaagg cttgatgtct
    14521 ttgccagaaa tttcaatgat cttatatgca atttccttga tggaagtaca gtgagaaggg
    14581 ccaatctgaa tatgaccatg accccaaccc ttatccaggg cgagcaccaa tgcatttacg
    14641 atatcattga catgaatgaa agcacgtccc tgctctccgc taccccacac attaaaaggc
    14701 tcttcaggat tattgattgc tttacgaatc agagcaggaa taacctgact acgttcgcca
    14761 aaatcagtag gtgtgccata aacattatgg agcatcagga tacagcacgg aattccggtt
    14821 tctttttcca aataccccat ctccaactgg cccatcagtt tgctccagcc ataagcagac
    14881 tcaggaagag cagggaacaa ctcctcttca cggagaggct caggattcaa agtattctgt
    14941 ctggtcagag ggaaggagca tacggtacca acatagataa cacccttgat tttctccttg
    15001 ccagccttac ggcaacaatc aaaaacatta gaattgatca gattattgat tcggaacaat
    15061 tcgccctggt tcttaaacac atagtcaatg ccagccacaa catcagccag atgaactaca
    15121 tactcagtcg taccaataac ctttttagcc tgctcataat cagtcagatc agcattgaag
    15181 aaatgagtat ccaggtcgat gacaggatgc ccatcttcgt cgttcaggta ctccaacttt
    15241 cctctccaca gattatcagc tacataaaca tcccagcagc cttccttcac cagtctctta
    15301 acaagattgg agccaatcat accgcaacca ccggtaacca taattgtctt tttcatttaa
    15361 cattcctcca aattttattc gtttccaaag aatttatctt ccagcatcaa acaccaacaa
    15421 tgatatatcg gcaagtgata ctcttgaatc atgtatgttt caacttcagg agccttaaca
    15481 caaacatctg ctaccgtggc aagttcccca ccctttgcgc ccgtcaagcc aataactttt
    15541 ataccgagag ccctagcaac aacagttgcg ctcataatat tcttgctatt tccggacgtc
    15601 gaaataccaa ggaatacgtc accagatcta ccaaagccaa acagctgctg tgcgaagaca
    15661 cccaaaccat cgacatcatt aatataagca gtagacaatg cttcgtgtgc gaccaaagga
    15721 attgccgtta aaccacactc gagatttttg gctaattccg gacctcttac agggtcaatt
    15781 gcaactaact tatcggcaaa ttccttactt acaggacgtg gcgtcttaaa ccgcttcatc
    15841 aactcgccag caatatgttc cgagtcagcg gctgatcctc cgtttcctgc cacaagaagt
    15901 ttaccaccat tcaaatagca ttcctccata attaggtatc catcgataat gtcctgttta
    15961 atcgtctcaa gtgtaggata tcgtttaatc aataagtcaa tatgtttatt aagtttttcc
    16021 gctaacattg ttactcctta tccattctgt ttttcaatat aagtttccgg cgtgtagtga
    16081 ataactctag ttccaccatt ttcaaattca aacggaatat acatcaaatc acgcatagcc
    16141 caacgaactg catcctgttt ctcaggttgt acatagaaaa ccaagaaacc tccaccacct
    16201 gcaccgagca actttccgcc taaagcacct gcagccatac ccttttcata tagttcatca
    16261 atgcttcctg tactaattgc tccaccagta ccttttttca atctccaagt aacatcaagc
    16321 tgtctaccga aatcatctaa atttctgttc ttatcagtaa gaattgcctc agcttcatct
    16381 acaagctcat acatcttttt cagtcttact ctcttatcct ctgtgccact gacgttgtta
    16441 gccttttgaa catcagaaga aaatctagtg aatccggtaa agaacatcat cagattgttg
    16501 ttaagctgct ttttacgttc aggcgaaata atgacaggca gcacctcata accatccaca
    16561 ttgaaattaa ttcgattgaa cccaccataa gatgcggcaa tctgatcctg ccatccaccg
    16621 gcttcattac acagaactct ttcaagataa atggcttcat cagcaagttt cttcttgtca
    16681 gcatacttac cctccagtgc ataaaaagca ttcaacatac caactgcaaa agaactggat
    16741 gtacccagac cagagcgagc aggtaaatca gcttcgtatg taagccgaat ttcatgcata
    16801 tcgagcattt tcatcgcatt acgaatcgca ggatgctcaa tatcttcaat gctagttact
    16861 ctctcagtac gagaataaga taactcagcg ctgtaatcaa aaaatctagg cagatgtctc
    16921 acattcacat aacagtactt atcaaaagta gtagaaagaa ctgcaccgcc attctctttg
    16981 aaataatcct ccatatccgt tccaccgcca aaaaaagaca tacgaaacgg tgtttttgta
    17041 ataatcataa atactctctc ctaatcttag tctctctgga agatatcacg ggtatatact
    17101 ttttccttaa tgtcatcaag gcaagcatcg tagcgatttg ctataacaga atcacacatt
    17161 gccttaaact gttccaaatc attcacaacc ttactgccaa agaaagtctc tccatctttc
    17221 aaggtcggct cataaatcac aacagttgca cctttagctt ttacacgctt cataacgccc
    17281 tggatagaac tctggcggaa gttatcagaa ttggatttca tggtcaaacg gtaaacacca
    17341 atcacaacag gcttttcttt gccagcatca tactgatttg cttctgcgta attgtaataa
    17401 ccagccatct gaagtacacg atccgcaata aagtccttac gagtacggtt agattcaacg
    17461 attgcctcga tcagattttc aggaacatct gcgtagttgg ccagaagctg cttggtatcc
    17521 ttcggcaggc aatagccacc gtaaccaaag gaagggttgt tgtaatgagt accaatacga
    17581 ggatcaaggc aaactccatt aatgatctgc tgagtgttca agcctttcat ctctgcataa
    17641 gtgtccagct cattgaagta gctgacacga agagccagat atgtattggc aaacagctta
    17701 acagcttctg cttcagtaaa gcccatgaac aatgtgtcca cattctcttt gattgcgcct
    17761 tcctgtagca gtgtagcaaa agtgtgtgca tcctcaacca aacgctcatc atccatatcg
    17821 gtacccacaa taatacgcga aggatacaga ttgtcataca gtgccttact ttcacgcagg
    17881 aactcagggc tgaaaataat attcttgcta ccagtcttta cacgaacaga agcggtgtat
    17941 ccaacaggaa tggtggactt aataaccata atggcatcag gatttacttc catgaccagc
    18001 ctaataacag cctcaacagc ggatgtgtca aaatgttgag tgcggctatt gtagttagta
    18061 ggagccgcaa taacaacaaa atccgcagac tggtatgctg cctttgcatc caatgttgcg
    18121 gtcagattca gttctttctc agccaagtac ttttcaatat aatcatcctg gattggagat
    18181 ttacggttat taatcagttc aaccttctct ggaatgatat ccaccgctgt aaccttatgg
    18241 tgctgtgcca acagagtggc aatactcaaa ccaacataac cggtgccggc gactgcaatg
    18301 ttatactgtt tcttcataat atttcctctt tccaccactg ctgtggtcac tactttctta
    18361 acaaaatgct gtttgtgaac agtaacctgc tatttatata aaacagcccg aaagctgtgt
    18421 tcattcatac ttaaaacagc cagctcctac gccggcaaat tcaatttttc ataaatcccc
    18481 tacccgttta gggttatatt gcaagtttga gtcattttta ttgacctcgt ttggggttat
    18541 aatacatttt cgcattgaaa tgtacctaca ttttccactc aaccagtcac caaaaagttt
    18601 ataaattcac ttattaccct ctattcttac tccaaaggta tgtttaacat cgctttattg
    18661 acctattttc accccaaatg gaagtttgtt tggtacatta ttggtctatt cccaccccaa
    18721 actgtctatt ttgagcgagt ttttgttttt tatcattgca cattttcaag gctcaaattc
    18781 taagcggatt tactggcagg gagacattgg taaatacttg aacaatttca cggctttcat
    18841 catcacatcc tacgataata attctaattg gataaagatt atcattaata atctactttc
    18901 acgtaaaaat tctgaactaa ataaaatcta ttgataatat ttttttcttc cactttttgt
    18961 atatcctaca ggtatagtgg atttaattat ccccaatgcc tctgaagtaa cagacatcac
    19021 taattcaata acacattcac taccatagtt tgtaggaaca aaaatgacaa caaaatctgc
    19081 ttccttatgt gatgtttttc catctgtcca aattttatta ccaatgaaat ctagtttaaa
    19141 atccctctta gaaactagtt cactctatat ttttattcag tttcattagt tctgtttttt
    19201 tgtcttcagt caccatatac atatatatct taattatcta tccatttcat cctacgcatt
    19261 cttgcgtaaa tacaattcac tctgttgagc tgatataaat cattggtggt acaaagataa
    19321 aatgataact gtttctatac ctccaaaaat tccagtaaat atatctatag atagtttcaa
    19381 aaaaaattgt cttagtttca taatatttaa gatagtggta catactttat aacaacgtac
    19441 ttttccaaat attattttcc gttatgaaca tccaatatat tacaaagttt aaaagtttag
    19501 atgaactata atcttcattt ctgcacagtg gataataaat tcttatagat aaaaaatagc
    19561 ctcttctaaa gtcacaaata ccttactccc acattttaag taaacactct gcagcattct
    19621 ctttgttata aaatgataga tattcctcaa ttttatctct tattatgtta ttatcagttg
    19681 ataccattcc attaatgttt cagaataatt acaatatttt gggaccggat aactcccttt
    19741 tgctcgtaac cactcatatc cgttaggatt cactaaaagt ttaccgttta tttttcgatt
    19801 ctttttttga accgtcccat gaacataccg attcaacaag ctaaattata aaaattggtg
    19861 tgctgtcatt attttttgga aaatttatta atggctatca atatactttt gtatcatact
    19921 aaatagctcg tcagttccta tatcggatat accaatatta aagcatttag atcattatat
    19981 ttaagagtgg tatcatccca aaatttcgaa tattttcttt tatataagaa taatgatact
    20041 gaattgcccc attcaccttt ctttttttag attttctaca agtgattcaa accctccata
    20101 attacagtta ttacttttga aacgataata tgaacatgtg taatcttttt tattaacaat
    20161 ccatcattct atccctaact aatatgctcc attgggcttg acaataacat aaaacgtttt
    20221 taaaatcaac ctcaagtcgt acattaaact tcgatgacta atatactcta aatccaactc
    20281 aaccatttta tggaaactaa cattatttct atcactaact tgccacaaac ctgaacaacc
    20341 aggttttaca tacaagcgtt gaagatcata tctcgtgtac tcagctactt ccctctccaa
    20401 aggtggccgt ggaccaatta gactcatatc gccttttaaa acattccata gctgtggcaa
    20461 ttcatcaatg cttttggcac ggataatttt acctatttta gtgacacggg gatcttcttt
    20521 catcttgaac attgcacctt ctatttcatt ttgatcaagt aattcttcca atttttcttc
    20581 agcatctaaa cacatcgaac ggaatttata cattttgaat gtttttccat ttttaccaac
    20641 acgtgtatgg caaaaaaata tcggacttcc tggctcttct tttctgattc gataagctac
    20701 atagaacatc aaaggagaag ctaaaatcaa accaataatg cttcctacaa tatcaatcat
    20761 tcttttaaaa aataaataga tatatcgttt ttcaactttt tctttatcca accaaacttg
    20821 attaggatgt acttcactaa tacgagataa taaatcttca ctttccatac tttccctctc
    20881 tcctcatttc ctttttatct acgaataaag aactaaaata aaccaaactt cttctttttt
    20941 acctcttcat agactggata actgatttca tccccgttga tcagatcctt tgcgacttgt
    21001 ttcatctgta ctactttttc cttaccaaag tccttagcaa tctgctcata gcactctttt
    21061 aagtaaaagg tacgcttaga gaccccatgg gcatcagaag cagccatctg cacaagatta
    21121 tgtttgacca tcattttggc tgttttctgg atgtcttttc caaatacacc gacataactt
    21181 ggtgcggtga gttgggccaa gcatcccata tctaggaatg gcagcatatg attcgggtct
    21241 ttacgaaaat gcgcgttgcg ttctggatgg acgatcactg gtgtctttcc tagttgtaac
    21301 aactcaaaaa agagctgttc tgcatataac ggcacttcca gtgtcggaaa ttcgattaga
    21361 agatatgtat catctaaatc tgtaaataaa atttcatctc ggtcgatgtc ttcgatcagt
    21421 gttccggtaa tacgtacttc ttgtccttcc aataacgtca aaggcaattg tcttttctct
    21481 aattctgctt gtaacgaagc gactagagaa atgacttgtg atttttcatt gctgtatttt
    21541 ccattattat gatggggtgt gcataaaata tgtgtgatcc cttgttgaat cgctttttca
    21601 gccatggcga tactggcatc tatgttttct gctccgtcat cgattcctgg caggatatgg
    21661 caatgtagat cgatcatttc tcttgtctcc tctcttttta tttcttaatt cccgtagtaa
    21721 tagccagcat ctttgctgtg ttctgcccca ttgtatacaa caccaaggac tcgcgcttgt
    21781 accatatcca gcaattcttt tgctttagtc aatgattctt tccgtgacat attttcacgt
    21841 acgaccaata aggtgccatc cgttttggaa gacatgatct gtgcatctgt tacggcaact
    21901 actggcggca tgtcgaagat cactacatca aatagttgtc ttgcttctgc taaaatctga
    21961 tccattcttg gcgaacttag tagttctgat ggatttggtg gtttaggtcc acttggcaga
    22021 atcgacaaat tctcgactgg tgttcgttgg atctcatctg ctacacttcc tgatgaacta
    22081 agaacggtac ttaatccgct tgcgttgttc aacttgaacg ttttatagat gacgggctta
    22141 cgtaagtctg catcgactag cagcacccgt tgaccagatt tggcaaaaac aacggcaatg
    22201 tttgctgcag tggtcgattt cccttctcca ggtcctgatg aagtcacaac aatcgtttta
    22261 atttgctgac cagctgcgga ggcaaactgg atattcgtcc gaatcgtacg atattgttca
    22321 gcgattggtg aagaggggtt tgtgagtgtg accaaactga ctgcatgagt ttgggctgct
    22381 ttttgtttct gtgttcgtgc catttttctt tcctcctaga ctcttgagcg gcttctgcgt
    22441 gatggcgctg ttttatcgtt tgtagctttt ggttgtgtcg gagctggtcc ttttttacgg
    22501 ataccagcat tcaattcttt cgctgacatt tctggtacag tccctaagat cgggaagccc
    22561 aatgtatccg tcacatattt ttcatctttt actgtccggt cgagtaattc aaataagaaa
    22621 gctagaccta cgcctaccat cattcctaac actaagccaa tcactagatt caatttatta
    22681 tttggcgaaa caggtgtcgt attggcaact gcacctgaga tgatcgagat tttatcgaca
    22741 ttcatcacgt ctttggcgtt ctcttggaat acttgtgacg ttgtattagc gatttgttga
    22801 gccgtcactg catttgtgct tgttgcttgg atcgagaaca tttgtgaatt ttgtgactga
    22861 ttgacggaga tggcatcttt gatttgtcca gcagtcattt ttacattgtc ttctgtttct
    22921 aaacgatctt tgacttcatt tagtactaga tcactgacga tcatatcctt atacgtattg
    22981 atcatttgca ggttcgtatt gacatcattg acatttgcag tatctgattg cggcagtgtc
    23041 acgatcaatt gggcttgtga gctgtatttt ggcgtcaaga tgaaaaacgt cacgatgcct
    23101 gagatggcca agccagcaaa cattgagatg aggattgtta tcacgtgttt gcgtaatgtt
    23161 tcaaaaattt ctttcaagct gattgtctct tccatttttt ctccactttc ctatttattc
    23221 tctagattca tttgatcttt taattctttt ttcacacgat caagttcttg atcgctgact
    23281 ttttggtatg agatattgtc ttgcatgaag ccttctcctt gcagctgatc ttgcttgatc
    23341 gtaccaaatg catcccgata atctaaagca atctttttca tttcatcaaa gctcatgtct
    23401 gttttcatat tcgactctag agcattcaaa acggattgat agttgcttac accgttcaga
    23461 ctcaacactt ttttcgctac accttctacg atctttcttt gacgttcttg tctgccgtag
    23521 tcaccatttg gatcttcata acgcatacga gaataagaca atgcctgatc cccgttcaat
    23581 gtgattttac cgatcgtaaa atcatagcca tcttgtgaga aggtgaggtc attattgact
    23641 tcgataccac caactgcatc aacgagttct tttagacctt tcatattgat tgaaacatag
    23701 tgatcgattg ggatgtctac atatttttgt acggtatcca tcgccatggc agctccgccg
    23761 aatgcgtaag cgtggttgat cttgtctttt gtgtcatgtc caacgatgtc gacatacgta
    23821 tcacgaggaa tgctgacaat cgttgtctgt ttgtcttcag gactgactgt ggcaaccatc
    23881 atcgtatctg atcttccttg atcgacccga cctaaatctc ccgtatcaat ccccatcaat
    23941 agaacagaga aagattcttg tttgtccaga tcaactggtt ttgttcgttc tccttgggtt
    24001 cgttccactg attcgtaagt tttttcaata gaatgtgaga gatctagata taattttgct
    24061 cctactccta cgattgctaa tactaatacg acaagaattg ctaaaataat tagaaatatc
    24121 tttttccctc ttgatcttct tcttcgtttt gccatagttt cctccgtttg ctagccttgc
    24181 ttgaattctc gcttattaat agctcgtatt gctatagccc atttgctgag cgactgtttc
    24241 tgcttttgac gcgtatgcgg ctcttatttt ttcattccgg atctgttcga tgagactgag
    24301 tgcggtcaaa tattgatcgt aagtagcagt agctgttact tgaccgtctt tcagcatcgt
    24361 atcgaatagc tgatctagtt gcgtgacttg cgcgatctga gtatcagcga atcctagata
    24421 ttgcataaca accgttttcc aaggactatc tttaaaaaag ctcatatttt cacgtacacg
    24481 agcgacatct gcacttgtca ccttttcttt gatgacgaca tcgtctacag cttgctgcca
    24541 gtttgataca tttttagtaa ataaatcgtt gacagctgtc tggatcttca gttttgattc
    24601 tgcatcctct aactgcgtta agactgcttt cttttcttct tggatcgcag cagcttcttt
    24661 aggtaattct gattccttga tgtcataatc atctgccgaa agtttgatag aagatagttt
    24721 atgtgacaaa tttgaaactg tttcttctgt tatttcagga tttaggaaaa ttttttcttt
    24781 tgtactgtac agcttatcta cttcttttga taaagaggtc acggtgtctt tttgattggt
    24841 gatggcagat tctgcgtaat tgatccgttg atgatgttgc cgctgattat tgaaatggta
    24901 tccaaacaag caaatcacaa tagcgcctgt gcctaccgcc acagtagcca ataacttgac
    24961 gaacggcttt tccatagatt cactcctaaa tattgatgat agataaataa agatgtgatt
    25021 cattcccatc agaatgagcg ctttttattt taaaagcata attaaactaa tatattcata
    25081 gaaatattta taaacaggtt ttccctatat aatcatacca caaaaagttt tcttgactag
    25141 ggaaattcct aatttaaaag taaataaaac agtaacaaga tcatgattaa tcgttttcat
    25201 ttttatttcc tctcttttca tgagcatttt gaaaattcga acaaagaata aaataacaaa
    25261 aattattaaa
//
LOCUS       DAZUMM010000036        24798 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000036 DAZUMM010000000
VERSION     DAZUMM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.036"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..429)
                     /locus_tag="ACG8E6_001409"
     CDS             complement(<1..429)
                     /locus_tag="ACG8E6_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5061833.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQ"
     gene            complement(532..876)
                     /locus_tag="ACG8E6_001410"
                     /pseudo
     CDS             complement(532..876)
                     /locus_tag="ACG8E6_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
     gene            complement(1001..1084)
                     /locus_tag="ACG8E6_001411"
     CDS             complement(1001..1084)
                     /locus_tag="ACG8E6_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFS5061834.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            complement(1133..1372)
                     /locus_tag="ACG8E6_001412"
     CDS             complement(1133..1372)
                     /locus_tag="ACG8E6_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFS5061835.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDMMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            complement(1492..3018)
                     /locus_tag="ACG8E6_001413"
     CDS             complement(1492..3018)
                     /locus_tag="ACG8E6_001413"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFS5061836.1"
                     /translation="MAICSKKQVKFRAMNYSGFFEVEASIKVNQGSFKGVLSPTQRFK
                     SQEELLSFVSSKQAKIGNQEGIIADVQTKEKKTNSPSLFSLSSLQSKVNQLYKATASQ
                     TLKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMVQTEPRKRY
                     VDGSKVQEHHAIIPTKQVPTESALAKMDDLQRKIYALVVKTTVAMFLPDYLYEETKIQ
                     TKVADLLFQSIGKTPKQEGWKILFKQQTKEEKEDVQTLPLVIIGEHAEVDVKSAEKET
                     QPPKAFTEGTLLTAMKTANKTVDDEEAIKILQEVEGIGTEATRASIIEALKQKEYIQV
                     IKNKLVVTEKGKLLCQAVESQHLLTSAEMTAKWETYLKKIGKREGNQENFINNIKKFI
                     VHLLEAVPNDIEKLNFSDYQEQKEKEAEKSIVGKCPKCGNNIVLKKSFYGCSNYPECK
                     FTLAEHFRKKKLTKTNVKELLEGKETLVKGIKNKEKKPYNAVVKIGEKGYIDFISFSK
                     "
     gene            complement(3170..4489)
                     /locus_tag="ACG8E6_001414"
     CDS             complement(3170..4489)
                     /locus_tag="ACG8E6_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSsu5 family transposase"
                     /protein_id="HFS5061837.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGRLSSDSGLVLVKELMNTFGFSH
                     LAKQWLHITDHRAYFTHDNLAILEQLIMQLIAGYSADSSANLLRRDPVFQAVLGKKQL
                     ASQSSISRFLDRFTEENIDQLQALNQSLIDKARLIRNDTELIIDVDSTHSDTFGRQEQ
                     TDYNAHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKAFINPLLHHYKSTLPH
                     TEILVRGDSGFATPEVYESCEATESQYVIRLKSNRRLSQLAEHSVLYGDNQEWEEREV
                     QYFSLPYQAQSWSKPRRVCIRSVREGGELLFHHAFIVTNLSDNVSPEVIFSLYGKRGT
                     MENFIKEAKGGFYFDKTDSPRFLENHVRMMISVLAYNLVNFLKSIGFDKVNQGMTIHS
                     IRLILLKVAGKLVQTGRQVYLKLSSYHVYQTEFYKVFERLRRSKQWI"
     gene            complement(4717..5244)
                     /locus_tag="ACG8E6_001415"
     CDS             complement(4717..5244)
                     /locus_tag="ACG8E6_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HFS5061838.1"
                     /translation="MESTDVINEIRGILPRNSIGKYDLLTIFTHKTVFDKIVKVLSLD
                     FQNKVDYVAAPEAIGWILGTAIAKELGVGFIGVRKGDKLPYAKEEIISTHFTDYSGQD
                     KSFEISKSSIVRNKRVLIVDDWIETGSQMKALIALLEKLDCSIIGLATIGIDINEVTQ
                     KWIDSSFVAYIGSNI"
     gene            complement(5288..6151)
                     /locus_tag="ACG8E6_001416"
     CDS             complement(5288..6151)
                     /locus_tag="ACG8E6_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFS5061839.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHLNQILRFELLRMMSWKVGIKTEFSLSVGKNYKYINKYID
                     EDLWNRLLSTYRMDSYENIWKSLFICHQLFREVSKEVAELLGFDYPEYGKNITRYTED
                     MYKKYVENDYF"
     gene            complement(6184..6918)
                     /locus_tag="ACG8E6_001417"
     CDS             complement(6184..6918)
                     /locus_tag="ACG8E6_001417"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5061840.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            complement(6899..7768)
                     /locus_tag="ACG8E6_001418"
     CDS             complement(6899..7768)
                     /locus_tag="ACG8E6_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS5061841.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            complement(7783..8007)
                     /locus_tag="ACG8E6_001419"
     CDS             complement(7783..8007)
                     /locus_tag="ACG8E6_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5061842.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            complement(8244..11231)
                     /locus_tag="ACG8E6_001420"
     CDS             complement(8244..11231)
                     /locus_tag="ACG8E6_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFS5061843.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLL
                     EQLDLPSSANEMSLWLKNDLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKE
                     DKSFRRRIQQLLPKIDLSDLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSE
                     SCNIGFSPVSKEGFDSLKYDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDG
                     HMASADGIRYITPQRSLYSRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSL
                     YLLEGFLNQPSELTPNQIMTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDAN
                     AHYGELDTLSQNSINMELIYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGK
                     AITEYGKVYKTKHQLRYISDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYD
                     GMEEQLNALGLVTNAIIYWNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVG
                     KYTFRYEAELSNGKLRPLNVEEF"
     gene            complement(11545..12030)
                     /gene="vanZ-A"
                     /locus_tag="ACG8E6_001421"
     CDS             complement(11545..12030)
                     /gene="vanZ-A"
                     /locus_tag="ACG8E6_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFS5061844.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(12183..13094)
                     /gene="vanY"
                     /locus_tag="ACG8E6_001422"
     CDS             complement(12183..13094)
                     /gene="vanY"
                     /locus_tag="ACG8E6_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFS5061845.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYETWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(13522..14130)
                     /locus_tag="ACG8E6_001423"
     CDS             complement(13522..14130)
                     /locus_tag="ACG8E6_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFS5061846.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(14136..15167)
                     /gene="vanA"
                     /locus_tag="ACG8E6_001424"
     CDS             complement(14136..15167)
                     /gene="vanA"
                     /locus_tag="ACG8E6_001424"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFS5061847.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(15160..16128)
                     /locus_tag="ACG8E6_001425"
     CDS             complement(15160..16128)
                     /locus_tag="ACG8E6_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFS5061848.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            complement(16343..17497)
                     /locus_tag="ACG8E6_001426"
     CDS             complement(16343..17497)
                     /locus_tag="ACG8E6_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFS5061849.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            complement(17475..18170)
                     /gene="vanR-A"
                     /locus_tag="ACG8E6_001427"
     CDS             complement(17475..18170)
                     /gene="vanR-A"
                     /locus_tag="ACG8E6_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFS5061850.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            complement(18384..18959)
                     /locus_tag="ACG8E6_001428"
     CDS             complement(18384..18959)
                     /locus_tag="ACG8E6_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5061851.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            19105..22071
                     /locus_tag="ACG8E6_001429"
     CDS             19105..22071
                     /locus_tag="ACG8E6_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFS5061852.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLQADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SEKVVSLNSLRPLKLS"
     gene            22212..22817
                     /locus_tag="ACG8E6_001430"
                     /pseudo
     CDS             22212..22817
                     /locus_tag="ACG8E6_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(22862..23029)
                     /locus_tag="ACG8E6_001431"
     CDS             complement(22862..23029)
                     /locus_tag="ACG8E6_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5061853.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(23063..23362)
                     /locus_tag="ACG8E6_001432"
     CDS             complement(23063..23362)
                     /locus_tag="ACG8E6_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFS5061854.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            complement(23403..24020)
                     /locus_tag="ACG8E6_001433"
     CDS             complement(23403..24020)
                     /locus_tag="ACG8E6_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5061855.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            complement(24345..24740)
                     /locus_tag="ACG8E6_001434"
     CDS             complement(24345..24740)
                     /locus_tag="ACG8E6_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061856.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         7409 a         4900 c         4160 g         8329 t
ORIGIN      
        1 ctgttctgtt aagctagaaa cgaaggactt agagacggat ttaccacaaa gttcttccac
       61 aatttttgat actttacgag ttgaaacgcc tgatacatac atttccaaca ttgaagccat
      121 gagggctttt tcgtttcgtt gataacgttc aaacactgtg ggtgaaaaat ggccatcacg
      181 tgttctgggt acttttaatt ctagcgtgcc tacacgtgtc gtaaagctgc gctcataata
      241 gccatttcgt tgactttgtc ggttttctgt tcgttcatat tcttttgctt gaatatattc
      301 tgttcgttga ttttccatta gttgattaaa taccgttgtt aaaatatttt tagaaacgtc
      361 atcctttaca gaatattcaa taatgctttg aatctcttcg cttttcagtg taaaatgtac
      421 ttgggtcatg taaaagtcct cctgggtatg tttttgtcgt taaaaacatt gtaccgtaaa
      481 aggactgtta tatggccttt ttacttttac acaattatac ggactttatc caaaaaccac
      541 ttttttaata atttgtgtgc ttaaatggta aggaatattc ccaacaattt tatacctctg
      601 tttgttaggg aattgaaact gtagaatatc ttggtgaatt aaagtgacac gaatgttcag
      661 ttttaatttt tctgacgata agttgaatag atgactgtct aattcaatag acgttacctg
      721 tttacttatt ttagccagtt tcgtcgttaa atgcccttta cctgttccaa tttcgtaaac
      781 ggtatcggtt tcttttaaat tcaattgttt tattatttgg ttgagtactt tttcactcgt
      841 taaaaagttt tgagaatatt ttatattttt gttcatgtaa tcactcctga agtgattaca
      901 tctataaata aatacagaag ttaaacgatt tgtttgtaat tttagttatc tgtttaaaaa
      961 gtcataagat tagtcactgg taggaattaa tctaacgtat ttatttatct gcgtaatcac
     1021 tgtttttagt ctgtttcaaa acagtagatg ttttatctac attacgcatt tggaatacca
     1081 acatgacgaa tccctccttc ttaattacaa atttttagca tctaatttaa cttcaattcc
     1141 tattatacaa aattttaaga taatgcacta tcaacacact cttaagtttg cttctaagtc
     1201 ttatttccat aacttctttt acgtttccgc cattctttgc tgtttcgatt tttatgatat
     1261 ggtgcaagtc agcacgaaca cgaaccgtct tatctcccat catatctttt tttgcactga
     1321 ttggtgtatc atttcgtttt gctttttgtg cgcctaaatt tcccacaatc actcacttct
     1381 ttctatttct tcttactctt attttatcat cacaaatcgc aagtgatttg tgatttgtga
     1441 taggtgattt gtgattaata tataaaagcc ctgtttaaag ggcttttatg tttattttga
     1501 gaaagagata aaatcaatat atcccttttc tccgattttt acaacggcat tgtagggctt
     1561 tttctctttg tttttgattc cttttaccag ggtttctttt ccctccagta attcttttac
     1621 attcgttttg gttagttttt tctttctaaa atgttcagct aaagtaaact tacattcagg
     1681 ataatttgaa caaccataaa acgatttttt taatacaata ttgttgccac acttaggaca
     1741 ttttcctaca atactttttt cagcttcttt ttctttctgt tcctggtaat cagaaaaatt
     1801 tagtttttct atatcgttag gtacagcttc cagtaaatga acgataaact tcttgatatt
     1861 attaataaag ttttcttgat tgccttctct tttaccgatt ttttttaaat acgtttccca
     1921 tttagccgtc atttcagcac tcgttaaaag gtgctgactt tcaactgcct ggcacaataa
     1981 ttttcctttt tcagttacaa caagcttatt cttaatcact tggatatatt ctttttgttt
     2041 taacgcttca ataatacttg ctcttgtcgc ttctgttccg atcccctcga cttcttgtaa
     2101 aatcttgatt gcttcttcat catcaaccgt tttattagcg gttttcatag cagttaataa
     2161 tgtaccctct gtaaaagctt tcggtggttg tgtttctttt tcggcactct taacgtcaac
     2221 ctcggcatgt tcgccaatga taaccaaggg taacgtttga acgtcctctt tttcttcttt
     2281 ggtttgttgt ttgaaaagaa ttttccaacc ttcttgtttt ggtgtctttc cgatcgattg
     2341 aaacagcaaa tcagctactt tcgtttgtat cttggtttct tcatacaagt aatcaggtaa
     2401 aaacatggca acggtcgttt taacgactaa agcatatatt tttcgttgta aatcgtccat
     2461 ttttgctaat gcagattcgg taggtacttg ttttgttggg ataatggcgt ggtgttcctg
     2521 taccttacta ccgtccacat aacgctttct aggctctgtt tgaaccattt cgagatcaag
     2581 tcctaaaaaa ccgctatatt tgccaaaatt tgcttttaaa taagcaaatt cgttctctgt
     2641 aataaatggt gtatctgttc ttggataact caataatttt gcttcataca gtccttgcat
     2701 agcttttaaa gtttggctcg ctgtcgcttt ataaagctga ttgacttttg attgtaaact
     2761 acttaaagaa aacaaactcg gactattcgt tttcttctct ttggtttgaa catcagcaat
     2821 tatcccctct tgattgccta ttttagcttg tttagaagaa acaaaggata aaagttcctc
     2881 ttggctctta aaacgctgtg tggggcttag aacgccctta aacgaccctt ggtttacttt
     2941 tatactagct tccacctcga aaaaacctga ataattcata gctctaaatt taacctgttt
     3001 ttttgaacaa attgccaatt tttaaattag gcagatactt tcttccaaaa taatgtgttt
     3061 tcgaacagga tgcctacgga agaagatcat aataaaaatt atttgtctat ttaagggcag
     3121 acgaatccct tgaaaaagaa tcggttaaaa aatttgtgtg ataactagtc taaatccatt
     3181 gtttggatcg ccgcaggcgt tcaaaaacct tataaaattc agtttgatac acatgataac
     3241 ttgacagttt gagatagacc tgtctcccag tctgaacaag tttaccggct actttaagca
     3301 atatcagccg gatggaatga atcgtcatgc cctggttcac tttatcaaaa ccaatgcttt
     3361 ttaaaaaatt aacgaggttg tatgcaagga cactgatcat cattctgaca tgattttcta
     3421 agaaacgcgg gctatctgtt ttatcgaagt aaaagccacc tttcgcttct ttgatgaaat
     3481 tttccattgt tcctcgcttg ccgtaaagag aaaatatgac ttcaggcgag acattgtcgg
     3541 atagattagt cacaataaac gcatgatgaa atagcaattc tcctccttca cggacggagc
     3601 gtatacagac acgacgaggt ttcgaccagg attgtgcttg ataaggtagt gaaaaatact
     3661 gcacttctcg ctcttcccat tcttgattgt ccccgtaaag aacagaatgt tcagctaact
     3721 gacttaacct ccgattgctc tttaatcgga taacgtactg gctttcagtt gcttcacaag
     3781 actcatacac ctctggtgtg gcgaagccgc tgtcaccccg aaccaatatc tcggtatgag
     3841 gtaacgtgct cttgtagtgg tgtaaaagcg ggttgataaa ggcttttacc cctttagatg
     3901 tgtactgatt gcctgaacgc agttcagctt ttaagaaatc tccggtcagt ccatcaaagg
     3961 caactaatgg gtgatagccg taggtttgat aatgggcatt ataatctgtt tgttcttggc
     4021 gtccaaaggt atccgaatgt gtggaatcga catcaatgat taactcggtg tcattgcgaa
     4081 ttaaacgtgc tttatcaatc aatgactgat ttaatgcttg gagttgatcg atgttctcct
     4141 cggtgaaacg atccagaaac cgtgagattg aggattgtga tgccaactgt tttttaccga
     4201 gtacagcttg aaagacagga tcccgtctta atagattagc tgatgagtct gccgagtaac
     4261 cagcaattaa ttgcataata agctgttcca atatcgctaa gttatcatgg gtaaaataag
     4321 cccgatgatc tgtaatatgg agccattgtt tagctaagtg cgaaaagccg aaggtattca
     4381 ttaattcttt aaccaggact aaccccgaat cacttgatag acgaccaccg gtatgtgaaa
     4441 tagtgatatt tgaattgaat ttaacttgat ttttgtgtaa gctagtcatg agaagaactc
     4501 ctttcttatg gttggtttcg tcacctttac catagcagaa tggggttctt ttttcatcac
     4561 ttaaggggtg aaactaaaaa agtaattatc agactgccgt taggcagttt cagacggttt
     4621 caattaaaaa gtatgaatta ttcagggtaa acatctttcg ttgtattgta aattaacgcc
     4681 atccaaaatt ttgattcaag attttctttc ggtttattat atgtttgagc caatataagc
     4741 tacaaaactg ctgtctatcc atttttgagt aacctcattt atatcaatcc caatagtagc
     4801 caacccaata atgctgcaat ccaatttctc taacaaagct attaaagcct tcatttgaga
     4861 acctgtctct atccaatcat ctacaatcaa aactcttttg ttcctcacaa tagaactctt
     4921 agatatttca aagcttttat cttgacctga atagtcggta aaatgtgtcg aaataatctc
     4981 ttcttttgcg tatggcagct tatcaccttt tcttactcca ataaacccca ctccaagttc
     5041 tttggctatg gcagtgccta gtatccatcc aattgcttct ggggcagcca cataatctac
     5101 tttattttga aaatccaatg atagtacttt tacaatttta tcaaaaacgg ttttatgtgt
     5161 aaaaatagtt agtaaatcat attttccaat tgagttacga ggtaatatac ctctaatttc
     5221 atttataaca tcagtacttt ccatacattt acaccccttg cttatggtta ataatctttg
     5281 ctaatcttta aaaatagtca ttttcaacat attttttata catgtcctcg gtatatcttg
     5341 ttatgttctt accatactct ggataatcaa accccagtag ttctgctacc tctttggaca
     5401 cttccctgaa caattggtgg catataaata atgacttcca aatattttca taggaatcca
     5461 tgcgatatgt agataataat ctattccata gatcttcatc aatgtatttg ttaatatact
     5521 tataattttt cccaacactt aatgaaaatt ctgtctttat cccaaccttc cacgacatca
     5581 tcctaagtag ttcaaaccgt agaatctggt tcagatgatc gattgcaaac agtatctctt
     5641 tgcggcacaa tcctttaata acataaggtg ttacattcca aaattcattg cagcaatcat
     5701 catactccct tgcgcttggc tttcttacat gataatctat atcagtcgga actatgtccc
     5761 ttttaattct acaatcttta tcaattagaa cctttattaa tttatcgccc tttaggtaat
     5821 tatctaactc ttccaagggc aataaggtaa gatcaatttt attgtaatca tcaaatagca
     5881 taagatagga aaatcccttt tcttcaggtg ggaataattc catatcctcc ggcttttgca
     5941 tcattattat attcccaaat tgattaagcc agtcatcatt agatataaac ggttctatat
     6001 cacttacaaa atatgtaata tcataatcct gaaattcatc tttaggtata ttaatatttg
     6061 cgcgtgaccc ctcaagggtc acaattcgaa tacgttcatc ctgttctgct aaagaaagta
     6121 ctaaatccat catttctttt tctgatctca tttacatact cctcgtttat tttttctata
     6181 ttattacatc ttcttttttg ccgatacaat cagcatcatt gggcgtcgca tttcatccgc
     6241 catccccgga atatccatca tgttctctgg cggctgtggc tccacaatct gatttattat
     6301 aaaactattt gaaagcagtg tatttagata tgtggtcagt gttctatgat attttgtaac
     6361 cttttcttcc aaaaacatag ctgtccgttt gccctcataa taataattgt ccaccgggaa
     6421 atgcagtatt tctccttttt cgttataata ccagtcttgt gttccatgag cagtaaaaac
     6481 aggatgttca actgtaaaaa ctaaattgcc accagccttc agcatcctat atatcttttt
     6541 tattaaattc tcatagtctg ctacataatg aaacgcaagc gaacttagta ttacatcaaa
     6601 gctctcctct gggaaatcca catcttctat ggcacagcat tcatattcaa tctgtggaaa
     6661 atgggttttt ccttttgcta cttcgagcat tttatgagaa atatcaacac ctactacaga
     6721 ggaagcaccg ttttccatcg catatataca gtgccatcca tagccgcatc ctaaatcaag
     6781 cacacgctta cccttaaaat caggtagcat ctttttcaaa gtctcccatt ctcccgcacc
     6841 agccagtcct ttctgcgagc gactcatttg actgtatttt tgaaaaaata tattatcatc
     6901 atatttgttt tctttcatct gaactctcct cgtttaaaaa gttatttatc tccgatacaa
     6961 tttcattcac ttctttataa agtttctcgg tcatatcgta gcattcaaaa agagaaagac
     7021 cgagtacttc aaaaatatga ttaactcttt cggcatattt ttcaggttta tattcaaaag
     7081 tttcaagcag ttttatagct ttcttttcgt taatgcaata agcattatta catgcaaata
     7141 gtacttgatt taagcatgaa ataatacgaa aaacatggcc tgcaatataa tatttatcct
     7201 ctacccccgc atttgctttt acaaacatta aagagaattc agcttcaaat ataaaaaagt
     7261 tcatcaagct tttctttaga gcagtagggt aaatttctgc ctgctttttt aattcgcata
     7321 agctttcgtt cttagcatat agtattttac taatcgctaa ttctccacga tacattgcac
     7381 ttatataacc atggggatgc ccggtctgat aattggcagt aacaattcct tgctccgtat
     7441 ctttgattat ttgttctacc cgttttatat cacgtaaaat caagtcaaca tgatacccat
     7501 ttataaataa ccatccgccg ccattaatcc aatcacccca cgctccggga ggcacaacaa
     7561 ggttgtttct atttttatca tccaattctg tagcaatttg attaatcgct gtcaggtcaa
     7621 atgagtctga attgtaatag attccgatat ctatatcaga attttctgta tgggtgcctc
     7681 ttgcacgtga gccccctaat acgatgcctt ctataaaagg caaagatgat aatttctctg
     7741 ttactatttg aataacatta cctaccatat aaacatcctc ctttattcgt tagatatttt
     7801 tatttcttct ccatctggta aaataaatgc ctgcacaaac ttgacaccta gcacatcagc
     7861 aatggccttc aactcatcca aggttactgt ttctcgttgt aattttttat tgaaattctg
     7921 tggagtctgg ccaatacgtc tagcaagttc ggaaacactt atattcattt gctcacacag
     7981 ttctttaatc atttctgccg tagtcataca gcacctccaa gtttacgcta agaatattat
     8041 aaaccatttg gttgataaac acaatagtta ttaaaatatg gggtacatgg aacatagaaa
     8101 cgaaatagtg cggtagcaca aaagtaaaaa cacaagaaac gttgatttaa cgctgttctc
     8161 gtgtttttct ctttttctat tcgaagtgat tcgctggaac agtcatacga aatagtgcgg
     8221 tagcatgcaa aacccgaaca ttgctagaat tcttctacat tcaatggacg taacttaccg
     8281 ttggataatt cagcttcgta tctaaacgta tacttaccaa caaaattgat gtgatcacta
     8341 attagtggag attgcctgcg tatcatttct tcggaacagt cgaatccttc agctttcatt
     8401 tgttcaaata ctttttctaa atacagtgag ttccaatata tgatggcatt agtaactaat
     8461 cctaatgcat tgagctgctc ttccatccca tcataataag tctggtagag ttttcctttt
     8521 cgtccataaa aaatattacg gcataaggca tgccgagctt cccccttatt caattgttct
     8581 aaaatttgcc gtgcatatat ttcgtcagaa atataacgaa gctgatgttt cgtcttataa
     8641 acttttccat actcagttat tgcttttcct aaaggcgtgg gttgcccatt ccgttgaaga
     8701 gatttcgtta attctgttgc attcactttt ccagatttca acgaacctgc aaccctaaga
     8761 atgtcctccc aatgggtata aatcagttcc atattaattg aattttgaga caacgtatca
     8821 agttccccgt agtgagcgtt agcgtcgatt cgccaaagtt tggttccttt atcattggca
     8881 attcttggac taaattgaaa ccctaataag ccaaagagac caaaaatcat atcgctataa
     8941 ccagctgtat cagtcatgat ctgattagga gttaattcgc tgggttgatt gaggaatcct
     9001 tctagaaggt aaagcgaatc tcgcagtgtc cctgaaacga ccattccgtg gaaccctata
     9061 tattgatcag agacaaaatt gtagaatgtg atcccacgac cacggccaaa atattttgga
     9121 ttacttctag aatataggga acgttgtggt gtgatataac gaataccgtc agctgaagcc
     9181 atatgcccat caccccatat cttagaggtg gcaagtttcc tgtgtgcatg tattattttt
     9241 tgattagctc ctgaaagtgt atccactcga atataatgat gattgacata gacaagacga
     9301 tcatacttta aagaatcaaa tccttctttt gaaaccgggg aaaaaccaat attgcaggac
     9361 tctgataaaa gaactgctaa taaactaata tccaagtctt tcattttggt gtccttgtca
     9421 cttaagtggt taaatgattg agtaagattt aactgttggt ttacttccaa taacaaatct
     9481 gacaaatcaa ttttaggaag taactgctga atgcgacgtc taaaagactt atcttccttt
     9541 ttttcatatg tctttttaag tctagagaca accaggcgat ctgcattttc tattctggcc
     9601 attttagaag taggccaacg atttagcgct tctttataag acagttccaa atcgttcttt
     9661 aaccataaag acatttcatt tgctgaagaa ggaagatcta gttgttctaa taaaacgtat
     9721 ttttgttgaa cccaagtttt ctcatcaatc aagcaatcca taggattgtt atataagaca
     9781 ctctgaggaa tataaatatc atggcgtttt aaagattgaa ttaacaattc gacagctgca
     9841 atcaatacac atttattagt ttgttcaggg tattcatgaa tgtaataaat ccatttcttt
     9901 ggaagagtat gtttaatagc tccaaacatt ttgtaagtaa tcggcttagg aaatctatta
     9961 ttaatgaagg tccagagttc cttacagttg ttaccgtagt cattgctgtc aaatgttagt
    10021 gtagttaaaa tatccggaat aaattttctg aactttcgat aagtgttcag aagttcagaa
    10081 atggctattg gttcacgatc atttttaacc aatagccgaa cttgcgatac agcttcggta
    10141 acattttcct cagaatattt ttctaagata cgatcgcgaa gggatagttc attagtatca
    10201 gaatgactgg acattactaa atctacaata tctgaaagtg tcgatgcggc tcgatctaga
    10261 tcttttatgg tacgaagacg ttctttagat tccttatttt tggcgcgtct aaaaacagtt
    10321 tcaaaaaaat tagatagtgc taaaatttgt tcatccatag ccttacgttt gtatatgaaa
    10381 ataaatgcca ctaacaacgc cagttttttc tgttcactca ttcgttgaat taattgagct
    10441 ttagctttaa atgcataatt tgcaagaact tttattttcc cttcaggaat agattccatc
    10501 ttccattttt cagtagagaa agcttgaaat tgctttagtc ggtcaaaacc tctggctatt
    10561 tctttatgac tatcgtcagc taacggggtt ctcaaaatgt ccattttgat tattgcccca
    10621 taaactggag ttccaattaa ttctaaatga ttgagaagcc gctttctctc atcttcagtt
    10681 ggaaccaaag acaattgtcg gtataaatgc tcttcagctt tttcaattgc actagcaaca
    10741 aaacgagaaa aagtagtaat tccaggtaaa acaatctttt catccagaca tttttttaga
    10801 agcatatcaa acaacatatc tgaagattct gttgtataca gcgctctatt taaaagccag
    10861 ttagctaggt atgtttctat ttccggttgg ttgaaatctt gatatccata atgtgatttt
    10921 atcaattttg tgtgctgata cctggtctgt ctcctgctat agtagataat ttctccagga
    10981 tcaatattta gttgagttgc tacataactc aatacatttt ggggaatatt ggtaagatct
    11041 aatataaaag ttcctaaaaa acgtagtgag actaactgta gtgcaaaacc taatctagta
    11101 gctggtcgtc gtatagtctc catcaattct aaatcttttt catgcaaata aaaaaattgg
    11161 tttaattgtt ctgtactagg ttctcctaaa taaatgccaa atgctttttt gttacttttg
    11221 atttcatcca tttaaaccct ccatgaagta aattttaagt ctacataaaa tttattttta
    11281 aaaatgctat gataggggta ccgtcaggaa aatgcggatt tacaacgtta aggaaattta
    11341 ttaaagagac caattcctca gcactaaaat tgataattgg gctttctttg ttactatagt
    11401 gttaggtaag ctttttactt tatcaccggt tttcccctca cttcacacct acgggcgagt
    11461 ttccccacat cccgttttcc atctaatcca attcattcaa tatctttatg tgtatgaaag
    11521 atcagattgc ttgatttaga catcttacac gtaatttatt cttaaatggg tacggtaaac
    11581 gagcaataat acgagcaaaa gtatacctac aaaaataata actctgtcta attttttttg
    11641 attcatatgc ttattgctta aaccatataa tttcagtcca agaaagcctc caacagtatt
    11701 tgtaattaca tctgttatgt ctgtcgctcc aatagcgaag ataaattgaa ttatttcaaa
    11761 agtaagactt aaaaccagta caaaagcaaa cttaggtaaa aatccgattt ctttaaaatt
    11821 gacattcaaa agcaagccaa atggaataaa gattataaca ttatctatca tctctctgaa
    11881 attcccagta gcagtaaatg gagtcaagtt aagacttctt tgatgataat taaatactga
    11941 taaaatattg tattgtaatt tgaataacac taaccagatt agtgtcacta aatataaagc
    12001 tagcaatcct ctagataata tttttcccaa tataatttcc tctaattctt ttgtttaagt
    12061 tgtatcatta gtatagcaat gtcctagtta acgtgggttt taaattaaaa agctaacgtt
    12121 accccaaaag tgctagtaca ataatgctgt tcattaattt cgttggtttc attccgccat
    12181 ccttacctcc ttgaattagt atgtgttgat ccgggaaaca ctgtcacaat tacaccgtct
    12241 atattgtttc ctgatatctc ataacgaaga ttagtcggca catgaatggt ggtattttta
    12301 gtaacaggat aataaaagat ctcatatttt tccccattta cactaacaga aatggttttt
    12361 tcttctttta ggtaatccat atattcctcg agaacgaaat tcttttcttt cataatcgca
    12421 ctatgtggta aaccaacata gcgaatatgc catgtttcat attgaattcc tgttaactct
    12481 gttttgtcct ctggataacg taaaatgaac ccgtatttcc aagcattttc ttctatccac
    12541 tttccttcag gggctcgttc cattttcgtc aagcttgatc ctacatctag tgataaacct
    12601 gaattatgct cactataacc tgctggtaag gcatactcag cccccatttc ttggtaaagc
    12661 acactttgct catcaaagtc tcgatagcca ctattaataa taaaatgact aacgccaccc
    12721 tttacagcat cattgaccat ctctgaaaat ttttgtgcta tttcttttga catataaata
    12781 ttactatcaa gcaacccgta tccatttatt aattcgtcat gtttagataa attcacgata
    12841 tctgacttca cactttcttg gcgaacagga tatttactat tgattaatag cagatttcct
    12901 tgataaacct gttcttctgt aattgttttc tcttgggtat tttcagaagt cccactattt
    12961 tctaaatgtt ctttgggatt ttgatcataa ttttgaaatt cgactttttc ctgagaaaac
    13021 agtgcttcat taacgtagtc ataacctaag tatattaaga ataacaataa caataaaaaa
    13081 aacaacttct tcattttcag tctcctcctt aactatatag ttggaagtat gaaatatttc
    13141 tagaatcttc cccttaaacg gcacaatctc gtttgagatt gtgccgtttt gccccgtaaa
    13201 ttacagtcaa ttatttcgcc ctactggatt tactattatc accaatgtag atgaattttc
    13261 ataagtgata cccatgctga atttaacgag gctgaactgc ctgttgacaa tgggcggtgt
    13321 gaaataccac ccctctatgc gatcagtctt attatcaggc tatcttgccg cagcgcacgc
    13381 agataatgac cgcaatatcg ctctgcctta catcttcaat actatctact atatataaat
    13441 tccctaagaa ccagttacat acgtcgggtt tcctgccgaa agagttagct tatatagttt
    13501 gtccgtgcaa cggttaaaag tttatttaac ggggaaatca aaatagctat tggggtatgg
    13561 ttcgtctctt aatacatagt gccaccattc gaggctatat gcttcaaacc cactgttttc
    13621 catgatggag cgcaaacgtc tgcgattttg cgcttcattg catgatattc catttgccgc
    13681 atgatgagag cgttcatcca taaaatcaaa tcggctcccc attggtacaa gctcacccgt
    13741 gtctaatcga taaagcgtaa gatcaatggc actgccgcgg ctatggcttg attttgaagc
    13801 cacgtatcct tttgaaatca tctcagttcg gtcaatattg ggataataac tttcctttgt
    13861 caggttattt tccggctgtg cagcccattg cataaaacag tttacagcac gcttaggacg
    13921 gtaaccgtcc catagaagca atccgtaccc ttgggtagca gccagttctt ttgccttcaa
    13981 aagcgattca gccaactcgt atgtccctac aatgcgattt acttcataac cgtcaaccgg
    14041 ttttccggtg aaattatccc aagtggcata tttagcgtcc caacgaacac cgtgtactat
    14101 ttcatctaaa aaagtaaatc ctatttccat gcttatcacc cctttaacgc taatacgatc
    14161 aagcggtcaa tcagttcggg aagtgcaata cctgcagcgg ccatcatacg gggataacga
    14221 ctgtatgacg tgaaaccggg cagagtattg acttcgttca gtacaatgcg gccgttatct
    14281 tgtaaaaaca tatccacacg ggctagacct ctacagccga gcgctttata tatttttttt
    14341 gccgtttcct gtatccgtcc tcgctcctct gctgaaaggt ctgcgggaac ggttataact
    14401 gcgttttcag agcctttttc cggctcgact tcctgatgaa tacgaaagat tccgtactgc
    14461 agcctgattt ggtccacctc gccaacaact aacgcggcac tgtttcccaa taccgcacaa
    14521 ccgacctcac agcccgaaac agcctgctca attaagattt tgctgtcata ttgtcttgcc
    14581 gattcaattg cgtagtccaa ttcgtccgcg ctattgactt ttttcacacc gaaggatgag
    14641 cctgaacgcg ccggcttaac aaaaacagga taggtaaacg tagctgccac cggcctatca
    14701 tctttattaa taacccaaaa ggcgggagta gctatcccag catttttcgc aacgatgtat
    14761 gtcaacgatt tgtccataca aattgctgag ctttgaatat cgcagcctac aaaagggata
    14821 ccggacaatt caaacagacc ttgtatggat ccatcttcac ctgacttgcc atgcaaagct
    14881 gaaaatgcta catcaacatg gttgatttca tattcatggt tctttttaac aagtaatccg
    14941 tgcatttttt tatccggcga gagtacagct gaatagcaat tgtcgttttc ccattccgcg
    15001 caaggttttt cgcacatttt ccatacacca gatttcgtaa ttccaatgta taacggctcg
    15061 tatttttctt tattaatgtt agcggctatc tctattgcag attttaccga tacgtcatgc
    15121 tcctctgagc aacccccaaa cagtattgca acttttattc tattcatgct cctgtctcct
    15181 ttcaaaatcc aaacagtttt taatggtttt ttcaacggta tcacgcaacg cttgctcggt
    15241 ataataggcc gtatgcggtg tgattatcac gttaggcatt ctttgaagtt taagtaaaaa
    15301 ttgattatca attggttttt gggtgcaatc agagtagaaa aactcttcct ctccttccaa
    15361 tacatccaat gcggcaccgc ccagtttccc gttttctaat gctttaacca actcataggt
    15421 atctacaagt ggaccgcgcc cagtattgat aagaaatgct ccttgcttca ttctctgtat
    15481 ttgttcgtgg ctgataatat agtgcgtatc cgtattgagc ggcacatgaa gcgtaacgat
    15541 atcgctattt tgcagcaact catcaaacgg tacatagttt acctctatac ttcggctgcg
    15601 actataagcc aacactttac atccaaatcc tcgcagccgc tcaataaccg ctttgcctat
    15661 ctggcccgtt cccaccacac caactgtcat gtcgctgagt accttgccac ggtcgctgtc
    15721 caacctgaaa tcatgttttt ccacagagcg cacaatcgat tttacgttgc gtactgccat
    15781 aagaattagc atcatagtat aatcggcaac gctatccggc gagtacgcca cattgtcgac
    15841 agtgatgccc attctcttag cagcagttgt atctatatga ttgcagccga tgcttcgggt
    15901 agaaatatat ttcacaccgg ctctcttcag cgcaagaaga atagaggcgg aaatctctga
    15961 tttatgtccc acactgatac attgattgaa aggcgcggat ttggcgttgg attccgacac
    16021 gttggcgtta attatcgttg ccataacgcc aaagcgaggc gaaagagcat ggaatgcatc
    16081 tgcctcatcc tgctcacatc cataaacagt aatgccgatg ttattcataa ttaagaccaa
    16141 ccctttctgt gaaaggcacc aaataaaacg gggatgccaa tggtaatctt gtcaaatctt
    16201 tctctgaaga acgaaaacgg ctcgttctta cggtattaag tctaccgtgt aagaacgagc
    16261 cgtttcttaa tattaaattg ttaatttcct aagaaaaagt aaagataacc ttgagatttt
    16321 cctaaaaaat tatatacatc tcttaggacc tccttttatc aaccaagtct ggcatcgctg
    16381 gaagctctac cctaaacgtc gtatagttat cattgctttc cgcgtaaatc tgccctccat
    16441 gctgaacaat aatttctttt gcaatcgcca atccaagtcc cgcgccaccc gtatcggaag
    16501 aacgagcatt gtccagccta tagaactttt caaatatggc agctagctta tcttttggga
    16561 tgcttccagt gttcttgaat tcgattgaca ccacatcccc ggagaggccc gcggtaatgt
    16621 caatgatgct gttatcctca ctgtatgcag cggcgttttt caaaatgttg ttaaagactc
    16681 tcgcgagttt atcagggtcg ccggacacgg tcagatcctc gggggcgtga ataaccgcct
    16741 gttttccatg tgcggaaagc tgaggataaa attcatcggt catctgcacc agcatatagt
    16801 ataggtctat gtgcgttttt gttagcgtta tcgtttgtag gttataccgt gtaatctcaa
    16861 aaaactcgtc gattagctgt tcgagtcgat acgctttgtc caacgtgata tgcacatact
    16921 ttgccttttg atctaccggc atgtctggag cctcgtcaag caggctcaaa taaccgataa
    16981 tggatgtaag gggcgtttta atatcgtgcg ccaagtacat aacaacgtca ttttttcttt
    17041 gttcggccag ctttgcatcc tgctctcgct tttccagagt ccgttttaat gtgttgagct
    17101 tttgttccat aacatccatt tccgcagaaa gctcaatttg tttatcttcg ttctgaataa
    17161 gtacatcaat gccggtattt atctcgtcaa agtattttgc gaattttgaa agcatgacgc
    17221 gacatagaat aagaatacta atgacaatcg ccacataaat aaagatatct atattgttcc
    17281 gtatggaata ttgatataat ttcatcgcgt ccaggtgatt taagtcatat ttgttttcca
    17341 aaatacttaa gatccaatcc ccaagtttcc ctcggatcat tgaacgaata tacaacacga
    17401 atacaattgc taccacaaca attgcaacga tatacatgta aagttttcgt tctagtttgg
    17461 aatagtcgtt ttttttattt ttcaatttta taaccaaccc cccataccgt ttttatatat
    17521 ttcggattat caatggtgtc gttcattttt tcgcgcaaat gccggatatg cacggtgatg
    17581 gtgttgttgc tcttgctgaa atattcgtcg ccccatatct catgaaatag cagctcggag
    17641 ctaaccacat tccccttgtt ttcacagagg attcgcagta ttgaaaactc ggtgggagta
    17701 agggataact gcttctcgtt cagataacac tcatgggtgt taacattaat gacaaggccg
    17761 gagtggacga taacattttc gttctgctcc tttactccac tgaatttttt gtatcggcgc
    17821 aactgggcct ttacccgagc aattaactcc agtgggcgaa agggcttcgt tatataatca
    17881 tccgcgccga ttgttaaccc tgtaatttta tctacctctg tatctttccc ggtcagcatg
    17941 ataatcggat aggtgtgctt gtcccttatt ttttgacaga tagtaaggcc gcttgtgccg
    18001 ggaagcatga tgtccaatat ggcaaggtca atctcagact tgtctataca ttccaatgct
    18061 tctttggcgg tatagtattt gaaaaccgta taattctcgt tttttaagta taattcaacc
    18121 aaatcggcaa tttcatgttc atcatccaca ataagtattt tatcgctcat agttatcacc
    18181 ctttcacata agtttgccct taataattta tcagattata gggccgaatt tataagcatt
    18241 tacataataa atgactaaga atttcttaag atgatgattc tttttttatc aaagggcttt
    18301 tcggcatcgc taggaggctg ttaatatttt atagtttcct tttcttcttt aaaaatattg
    18361 cccattagcg gaatacagaa tggctaatta ttcacttctg ataatttcct gtataatgaa
    18421 gccctagata cattagtaat ttcacaaatt tgatttacag tcatatttcc ttctttatat
    18481 agctttaccg cataattcat tcctgcgtga tttttatgat acttctttaa tcgaccttta
    18541 aactttcctt ctttcttagc caattcaatc ccttcacgtt gtctcatccg aataagatct
    18601 cgctctaatt ggttaacacc agccattaca gtaattaaga attggctgta tggattatct
    18661 tctgataaat caagccatgt atcttttagt gattttaaac ttgccttttt atctcgtatg
    18721 ttatcgatta attcaaatag atcttgtgta ctacgagtga ttcgagttaa gtctgtaaca
    18781 taaatgatgt catcttcctg taaatcgtct aacacttttt gaagttgctc gcgatccttt
    18841 gttgctcctg aaactttctc ttcatatata atatccattc cgatctcgtt caactgctga
    18901 aattgtcttg aaggattctg gttagtcgaa ctgacacgaa tataaccgat tttccgcaaa
    18961 atatcacctc atttttgaga caagtcttat gagacgctct taactatgat tttatcagtc
    19021 tactacattt gtatcaatag agtacactct attgatatat aattgaacta ataaattgaa
    19081 aatacagaaa tggaatgata ctgaatgaaa attgcgagag gtagagaatt gcttacaccg
    19141 gaacagagac aggcttttat gcaaatccct gaagatgaat ggatactggg gacctacttc
    19201 actttttcca aacgggattt agaaatagtt aataagcgaa ggagggaaga aaaccgttta
    19261 ggatttgctg ttcaattagc tgttcttcgg tatcccggtt ggccatacac tcatatcaaa
    19321 agcatcccag attcggtcat acaatatata tcgaaacaga ttggtgttag tccatcctcg
    19381 cttgatcatt atcctcaaag ggaaaataca ctttgggatc atttgaaaga aattcgaagt
    19441 gaatacgact ttgtaacttt taccctgagt gaatatcgaa tgacatttaa gtaccttcat
    19501 caattagctt tggaaaatgg tgatgccatt catctactgc atgaatgcat agattttcta
    19561 agaaaaaaca aaattatact gcctgctatc actacacttg aaagaatggt gtgggaagca
    19621 agggcaatgg ctgaaaagaa gctatttaat acggttagta aatctctaac aaatgagcaa
    19681 aaagaaaagc ttgaagggat tattacctcg cagcatccat ccgaatccaa taaaacgata
    19741 ttgggttggt taaaagagcc accgggtcat ccttcacccg aaacttttct aaaaataata
    19801 gaacgactcg aatacatacg aggaatggat ttagaaacag tgcaaattag tcatttgcac
    19861 cgtaaccgcc tgttgcagct gtctcgctta ggctcaagat acgagccgta tgcattccgt
    19921 gactttcaag aaaataaacg ttattcgata ttaaccatct atttattaca acttactcag
    19981 gagctaacgg ataaagcgtt tgaaattcat gataggcaaa tacttagttt gttatcaaaa
    20041 ggtcgtaagg ctcaagagga aatccagaaa caaaacggta aaaagctaaa tgagaaagtt
    20101 atacacttta cgaacatcgg acaagcatta attaaagcaa gagaggaaaa attagacgtt
    20161 tttaaggttt tagaatcggt tattgaatgg aatacctttg tctcttcagt agaagaggct
    20221 caggaacttg cacgtcctgc cgactatgat tatttagact tactgcaaaa acggttttat
    20281 tcactaagaa aatatacgcc aacgctatta agagtattgg aatttcattc tacaaaggca
    20341 aatgagccac ttttacaagc tgttgagatt atccgaggaa tgaacgaatc tggaaagcga
    20401 aaagtgcctg atgactcacc tgtggatttt atttcaaaac gatggaaaag acatttatac
    20461 gaggatgatg gtacaacaat taatcgtcat tactatgaaa tggctgtttt aacagaactt
    20521 cgggagcatg ttcgggcagg agatgtttcc attgttggca gcagacaata tagggatttt
    20581 gaggaatatt tgttttcgga agatacatgg aatcaatcga aggggaatac gagattatca
    20641 gttagtttat cattcgaaga ttatataacg gagagaacca gcagctttaa tgaaaggtta
    20701 aagtggttag ctgccaattc caataagtta gatggggttt ctcttgaaaa aggaaagcta
    20761 tcacttgcac gcttagaaaa agatgttcca gaagaagcaa aaaaatttag tgcaagcctt
    20821 tatcagatgc taccaagaat aaaattaact gatttactca tggatgtggc ccatataaca
    20881 ggatttcatg agcaattcac tcatgcttcc aataatcgaa aaccagataa ggaagaaaca
    20941 atcattatca tggctgccct tttaggaatg ggaatgaata ttggcttgag caagatggcc
    21001 gaagccacac ccggacttac atataagcaa ctagccaatg tatctcaatg gcgcatgtat
    21061 gaagatgcca tgaataaagc ccaagccata ttagtaaact ttcatcataa attacaattg
    21121 cctttctatt ggggcgacgg tacaacatct tcgtcagatg gtatgagaat gcagctaggt
    21181 gtttcatcac tacaagcaga tgcaaatcca cattatggaa ctggaaaagg agccaccatc
    21241 taccgattta caagtgatca attctcttct tactacacaa agattattca tactaattca
    21301 agagatgcga ttcatgtttt ggatggtttg ttacatcatg agacggatct aaacatagag
    21361 gaacattata cagacactgc cggttacact gaccaaatat tcggactgac tcatttatta
    21421 ggatttaaat ttgccccaag aataagggat ttatcggact caaaattatt tacgatagat
    21481 aaagcaagtg agtatccaaa actagaagcc attttacgtg gacaaataaa tacaaaggtc
    21541 attaaagaaa attatgagga tgttttgcga ttagctcatt ctataaggga gggaacagtt
    21601 tcagcatccc ttattatggg gaagctaggt tcctattcaa gacaaaacag cttagctaca
    21661 gccttacgtg agatgggccg aatagaaaaa acgatcttta ttttgaatta tatatcggat
    21721 gaatcattaa gaagaaaaat acaaagagga ttgaataaag gagaagccat gaatggattg
    21781 gcaagagcta ttttcttcgg aaaacaaggt gagcttagag aacgcaccat acagcatcaa
    21841 ttgcaaagag ccagtgcttt aaacataatt atcaatgcta taagtatttg gaatactctc
    21901 cacctaacaa cagcagttga atataaaaaa cggacaggta gctttaatga agatttgtta
    21961 caccatatgt cgcccttagg ttgggaacat attaatttac taggagaata ccattttaac
    22021 tcagagaaag tagtctcatt aaattcttta agaccactaa aactttctta acgttgttaa
    22081 aaacgaggga ttcgtcagga aaataggctt agcgttgtaa atccgcattt tcctgacgct
    22141 acccctgata gcagtaaaac tacaagtaaa ttttacatgt aaattatatc acttttaaaa
    22201 aggagttttt tatgggaaaa attggctata ttcgagtatc ttcacaagat caaaatttgg
    22261 atcgacaatt ggcaatgatg gaaggactta acattgataa attattccaa gaaaaagcca
    22321 gtggtaaaga tactgagcgt cctgagtttc aaaaattact aaattatatc cgtgaaggtg
    22381 attgcgtcat cgttacatca ctagatcgtt taggaagaga ttatgaagat ataaaaaata
    22441 cagttgcttt tatgaaacag aaaaaagtag cgttaaaaat attagatgca cagtttttag
    22501 attttaatac tggaaatgaa ctcctggata ccgctatgtt cgatatgttt ttatcactgc
    22561 taagttatat tgcacaaaac gagcgtgaga aaattcgtga acgtcagcgg taaggagtat
    22621 tattggctca aaaagccggc cgatataagg gacgaccaat cgagtattct attaactcaa
    22681 cagatccgca aaaacgctta gtttttaaaa ccgtggtgga catgttaaag caaggcattc
    22741 cagttgcaga gattgcaaaa gaaaacggga tctctcgacc aactatttat aaaataaaga
    22801 aagcgaatga tttgtagaaa tatactacat tcattcgctt ttctaccgca ctatttcgtt
    22861 tctatgttcc atgtaccccg tatcggcgat ggcatttagg acagtaaaag taattttttt
    22921 gataaacact ttttgctaat tccccacagt cacactccac gtgaaaagca atatttcgat
    22981 taaaactagt tatttcaatg ccccaacgat cataagattt tcgcttcata aaaattttcc
    23041 tccttgtaaa ttttactaac gattataaat accgttgcca ttctttcaac tgttgactaa
    23101 ttaacgcata aaattcgact tctgataaaa tggtaatctc atgacctaac gcaatcaagt
    23161 cccgcgcggt ttgttccttt tttgtataga gagaatcatc aaacaacgac ttagaatact
    23221 gaccacaaac caggaaattt gtttttgaag taacacttga ttgacaatgg ccgtttaaag
    23281 agtgaaccaa accgatggct tgggttcgtg tcatttgctg cagcgttcca gtaaaaacga
    23341 ccctttgatt cgttaagtcc attttcaaac tctcccttcc cgtctaatag ctgttatatc
    23401 tatcaagaaa agtggtgcaa ggtatgttca tattctttca cccgtcgata aaaggtattt
    23461 cgtttcatgc ccaattgatc catagcatag actgcagtta tctttcctgc tttccagtca
    23521 gaatagatcg caggaaattc aggaggtaaa gcaattttct ttctgccgaa ttttacaccc
    23581 ttttgtttcg cagcttctac gccttctctt tgccggctat taattcgttt ccgctcgtct
    23641 tcggccatat agctgagtaa ttgcagaatt aagtcagaga taagcgtctc taacccatct
    23701 agttgcttgt aactagcagt atctaacaga ggcatgtcta acacagaaat atcaatctgc
    23761 ttttccttag ttaattctcg ccattcttct aaaatcattt gtttatttct gcctagtcta
    23821 tctaatgatt gtagatagag taaatctcct tttcgaaaca tccgtttcat taattgatat
    23881 tcagggcgtt taaaatcttt gccgctggct ttttctacaa atatatattc ttcagggatt
    23941 cctaattcct tcgcggaaat cagttgtcgt tcgatcgatt gatcataact actgacccgg
    24001 atataaaaaa attttctcat cgtttttatc tacccttttt tcactaaaat tgtttcaaaa
    24061 caatcagatt aaatcagaaa cgtttcaaat tgtattatgt ggttattatg ccatacataa
    24121 acaaagaaaa tcaaggcatg tcaaaaagta tactttttga aacagtaagt ttacactccc
    24181 gcatgattat atttattccg ctaaaataca taaaaatggc aggccttaac ggtctgccat
    24241 taaataaact agttatccag tttttctttt atgtcttcca tggtatattt tatttttcac
    24301 atcagaattt gacttcttta acttttacaa tttttttaac actattattc tattacaaca
    24361 ctatatattt ctaatttagg gttaaaaata aatctttttg atacttttgc ctttacaata
    24421 taattgtatt ctttcttttt ttctttgaag tcgcttgatt caactgtaac ttcaaaccac
    24481 atttcatttg gagactcttt taattttttt acttgaaatg aattatctct tggatcataa
    24541 ggtggtactc tttcaaactc aggaagtttt ctctgtattt gttcttcagg agtattcgca
    24601 aagtaatcta ctatcttgac agttattaaa agtactacta ttcccaaaac tataacaaac
    24661 ataaagataa ttccacatcc agaattattt ttattatttt gagtcagatt attccattct
    24721 atttcttcca taatcatcat gtccttttac aattcatata cttattttat cgtatatatt
    24781 taaaaaggaa aaggacac
//
LOCUS       DAZUMM010000037        24747 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000037 DAZUMM010000000
VERSION     DAZUMM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.037"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..104)
                     /locus_tag="ACG8E6_001435"
                     /pseudo
     CDS             complement(57..104)
                     /locus_tag="ACG8E6_001435"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(418..1332)
                     /locus_tag="ACG8E6_001436"
     CDS             complement(418..1332)
                     /locus_tag="ACG8E6_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5061857.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            complement(1347..2348)
                     /locus_tag="ACG8E6_001437"
     CDS             complement(1347..2348)
                     /locus_tag="ACG8E6_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS5061858.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            complement(2345..4528)
                     /locus_tag="ACG8E6_001438"
     CDS             complement(2345..4528)
                     /locus_tag="ACG8E6_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS5061859.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            complement(4531..6978)
                     /locus_tag="ACG8E6_001439"
     CDS             complement(4531..6978)
                     /locus_tag="ACG8E6_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5061860.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            complement(6962..7354)
                     /locus_tag="ACG8E6_001440"
     CDS             complement(6962..7354)
                     /locus_tag="ACG8E6_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5061861.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            complement(7441..7938)
                     /locus_tag="ACG8E6_001441"
     CDS             complement(7441..7938)
                     /locus_tag="ACG8E6_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS5061862.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            complement(8055..8279)
                     /locus_tag="ACG8E6_001442"
     CDS             complement(8055..8279)
                     /locus_tag="ACG8E6_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061863.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            complement(8383..10293)
                     /gene="ltrA"
                     /locus_tag="ACG8E6_001443"
     CDS             complement(8383..10293)
                     /gene="ltrA"
                     /locus_tag="ACG8E6_001443"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS5061864.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(11077..12282)
                     /gene="mobT"
                     /locus_tag="ACG8E6_001444"
     CDS             complement(11077..12282)
                     /gene="mobT"
                     /locus_tag="ACG8E6_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS5061865.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(12305..12458)
                     /locus_tag="ACG8E6_001445"
                     /pseudo
     CDS             complement(12305..12458)
                     /locus_tag="ACG8E6_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(12461..13846)
                     /locus_tag="ACG8E6_001446"
     CDS             complement(12461..13846)
                     /locus_tag="ACG8E6_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS5061866.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(13875..14261)
                     /locus_tag="ACG8E6_001447"
     CDS             complement(13875..14261)
                     /locus_tag="ACG8E6_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5061867.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(14277..14591)
                     /locus_tag="ACG8E6_001448"
     CDS             complement(14277..14591)
                     /locus_tag="ACG8E6_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5061868.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(14944..15423)
                     /gene="rlmH"
                     /locus_tag="ACG8E6_001449"
     CDS             complement(14944..15423)
                     /gene="rlmH"
                     /locus_tag="ACG8E6_001449"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFS5061869.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15851..17140
                     /locus_tag="ACG8E6_001450"
     CDS             15851..17140
                     /locus_tag="ACG8E6_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFS5061870.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(17179..17784)
                     /gene="ytpR"
                     /locus_tag="ACG8E6_001451"
     CDS             complement(17179..17784)
                     /gene="ytpR"
                     /locus_tag="ACG8E6_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFS5061871.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(17980..18444)
                     /locus_tag="ACG8E6_001452"
     CDS             complement(17980..18444)
                     /locus_tag="ACG8E6_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5061872.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(18466..18786)
                     /locus_tag="ACG8E6_001453"
     CDS             complement(18466..18786)
                     /locus_tag="ACG8E6_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS5061873.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(18929..20008)
                     /gene="pepA"
                     /locus_tag="ACG8E6_001454"
     CDS             complement(18929..20008)
                     /gene="pepA"
                     /locus_tag="ACG8E6_001454"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFS5061874.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            20208..20558
                     /locus_tag="ACG8E6_001455"
     CDS             20208..20558
                     /locus_tag="ACG8E6_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS5061875.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            20743..21696
                     /locus_tag="ACG8E6_001456"
     CDS             20743..21696
                     /locus_tag="ACG8E6_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5061876.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            21709..23496
                     /locus_tag="ACG8E6_001457"
     CDS             21709..23496
                     /locus_tag="ACG8E6_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6020 family protein"
                     /protein_id="HFS5061877.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            23483..24685
                     /locus_tag="ACG8E6_001458"
     CDS             23483..24685
                     /locus_tag="ACG8E6_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061878.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         6992 a         5007 c         4518 g         8230 t
ORIGIN      
        1 gcatcaacat gagctaaaac tccaatatta ataattttca tgtgattttc ctccattcaa
       61 aagcccaaaa gggcataaaa atcccagtga taaatactct tatcactggg atttttatgc
      121 ataaccatag gcatacaaag catacagata ttctccggat actttagaat cacatgataa
      181 aggtattctt aaactgggtg caaaaaacta agccctccta aaaaaggaca tccaattatt
      241 tgttcccact atcaaattga cagtttattt aagaatacct tgccgcatat ttattaactc
      301 cttttaaata gatacttaaa taatagcacg taagagcata tttgtaaagg aatctccaat
      361 tttttatcaa agagagtacg tgattacaaa atagctgtaa taatgtacca atatttgtta
      421 ttctataatc ttccaattac tcccgttctt ttcaagtacc aaatcaaatt gagatacctg
      481 cgttgctttg gtctgctggt cgatatactc cactgtcagc gataccgtga cttgattatc
      541 cttacgattg taaataggat ttaccagttc ttgaaagatg tactcttttc cgattggttt
      601 taatatcccg tcattcacat agtaggaaag ttcactggct gtcgctgtag gatagagctt
      661 gaagaacgtc gttaaaaact cattgatttc attggttgta atggaatcaa ccgtaccctc
      721 actttcaata gcttttggtt tatagtctga tttctttggt atgctagtaa tggtcggatt
      781 cttaatgagg accatatttc cagtactgtc tacatagaca ctcactatat aagcagagtg
      841 gacggtcttt gtattttccc cctctgtaat gagttggtct acactgtagg ttacatcaaa
      901 ttgattgtcg ccatctgctt ctaccgtcca tatctgaaat ccttttacag aagacgatac
      961 aggaatatct ttacgaactg tatcgacatt cagagcttga agttcctctg tcagatatgc
     1021 tttcaggttt tctatgcgat tatcaatgga cttgtcgttt agttcccatg aatagtagac
     1081 tttcgcaaag ttctctacaa aattttccac atgatgagta tcgacatatt ccttttctat
     1141 gatggtggtt tcatgaatgg tatgggtatc aatggctgta aagtgcttga atatcgcaaa
     1201 gctgaaacta agtcctaaaa gtacccatag gacaatcaca acctttttac gaggattgac
     1261 cttatagaca cgaggtttct tttcctttgg tgtctgtttt tctgtattct gattttttcc
     1321 gaatttcatc attgaatcgt cctttcttat tgtttgattc gtcctgctcc cactaaatgc
     1381 tgttgccagt aagagcttgt taagtcggca taaccgatag ggtcgcctgc atgaaacata
     1441 cggttatcgc ctaaataaat cccgatatga gtaatataag agccagcgtt ataggtagaa
     1501 tgaaagaaaa ccaaatcgcc ggcttttgct tccaataatg ggatatgccg ggttacatca
     1561 tattgctgtt gtgctgttcg tggtaagttg attccagctt ttccatacgt ccattgtgtc
     1621 agtccgctac aatcaaaaga ggtagtcggg gaagctccgc catagacata cttccagcct
     1681 tcaaatttca gagcttcgtc catgatggct tgtactgtat catcatcaaa ctctgttgtg
     1741 acaagatact gcgttaccag ttgcacataa aacatattcc cgtaattgta tcgccagccc
     1801 ccattgatag gtatggctat gggattgggg taagacactt tttcgtcacc cgaatactct
     1861 tttgagaaac tttgagccag ttcaaaggta tatttattcc cacgattagc cacatatcct
     1921 aagaaaccac caccataatt gtaggactgg ataaccgatt ctaaatctac accaagcctt
     1981 tcgctactgg ctaataactc actgaaatac ttcacacctt gcttaatgga ttcttctgta
     2041 ctcaatgaat taggtggaag accgagggat tccgaggact gcataacatc ttctgctgta
     2101 ccacccgatt ccacctgtat aatcgcaaga agtatattga cgtattcttc aattccatat
     2161 tctttggcaa ctttttctac cataggctta tgagccagca cttctgcgga aacatccaca
     2221 ccgccatagt gaatgttgga aaaaccgcca tcttgttcat ctgaaaataa aatggcgaca
     2281 aacagaagca gtgagaagac catcaagaat aatcccgaac caccaatcac taaagttttc
     2341 agcttcatgg tttcttactg cctttcttaa tggtggcggt tttgattggt ggtctacttt
     2401 ttgtattttg tagtggtact ctttgaacag tagactgacg ttcttttgtg attgaacgtt
     2461 gtgaagctct atctgttggt gcagttgaag ttactggctt ttgaaccgtt ctttcttgaa
     2521 ctgtattact ttgacgttcc gtttttaaac ttgaagaatc ggacttaact gttggacgct
     2581 cttgtttggc ttgttgagat tccctatagg aagcctgtac agttgtttgc cttgaagtct
     2641 gtccatcatg agattgttct tgcttagtgg ctggtctttc atgaacggaa gaagctggct
     2701 gttttttctg tttgacctgt tccatttcag agcgacgctc cgcaatggtt ttccgtcttt
     2761 gttcctgctg tttcttgcgt ccactggctt tatccgcttt ggtttgagaa atacttctgg
     2821 ttaaatcatg gacattatcc gtcactttag attttccttg atatactgca tatcttgcat
     2881 tggtcggcaa atccttaacc tgttctttca aattaccagc agtatctacc attctgtctt
     2941 tggtatcagc tactgtaccg atggtttgac cgatacgctt tcctagtgtt gatttttcct
     3001 gcccgtttgg tcgagagtga tctgcttgtg tactcgcaga acttcccgaa cccgactgtc
     3061 cttttttacc cgtagcactg gcaatggcag acccagcccc taaagcagtc acggagcgtc
     3121 caagtttccg ctgtagacgg tgcatgtgag cgtgcataag catacgaggt tttctcatca
     3181 tacgacttcc cacactttga gaatcgttac tctgtagaga aaacatactc attaaatcgc
     3241 ccagcttaaa gtagattcct gcaaaggtca caatctgtag aaaagcaatc aaaaagaacg
     3301 gataaccagc cgataaggta tagagcatgg ttgaaatact aaatgctgtc gtaataatca
     3361 atgtgattcc agctcgtgtc aaaatggtat taaagagctt tgttatggct cgttttgaca
     3421 taccatcaaa tgatggaatc atgcttaaaa taaagctcac aggcagaaac atagcataga
     3481 tgataaaaag tacctgcgag aaaatcatga ttcctgttaa taggaataca aatatggaaa
     3541 tcccaatatt gaagacaaat aggaagaaga ctgtacctaa acggttaatc gtctttgtaa
     3601 tcgtcatatt ggtattgctt ctatcttcaa tttcttccgc aacaattttt tctctgtctt
     3661 ccccattgtt ggaatctgga ctggttgaga gcaggctttc cacacggtca ataccaatac
     3721 tttcaatgtc tgaactgtta tattgaagca gtagccacgg ttgctgaacc tgtatggaaa
     3781 ataggctgtc acgtatcaag tccacactgt ccttgccttg actatctgaa tggggcatga
     3841 caatttttgt acccagtgac aaactagcgt tgctgatgtc tgatgaaaag tcattgattt
     3901 ttttaatgta gtcgggagcg taggcaataa aggaagccga taggataaaa accagcacaa
     3961 aattcatgag ggcatgaatt gcctttgtgg tttctctctt tatcagtcct gtataggcaa
     4021 cataaacccc aagaaccaaa atcaagagta aaaggaatcc aacatagaaa ccctctgttg
     4081 atagtccact aggcgtaacc cctgcaaggg tctgcatatt cttgccaatg gaatctgctg
     4141 tagcggaaat gaagtctaag gaataggctt cctgtactaa gtaacccgtc gcattggaaa
     4201 cataaagact gattgtccaa ataaaattgg taatggcata tagtccatac atgacttgtt
     4261 ttccaattcc gtccgaccag ttccacggta gccagcccca gctattatcc acataaaaat
     4321 ccagttgata gttttcaagt gggtatcggc tgtattcatt tgccacattg accgtatcat
     4381 ctaccaagcc cgcagcttga accaccgttc ccagcatggc taaaagaaaa atggcaatca
     4441 caagtgtgaa agccactgtc attgccactt tacctagacg tttcagcgtc cagtttgatt
     4501 ttattctgtt taatattgat ggtttcacat ttacacctct tttcgcacag gtggtctggt
     4561 atcaaaggca tggagcagtt cttcaaatac agggtggaac tgtatcacac cgacacgacc
     4621 atataaatca ctgataaggc attgcccgtt ttccaaatca cgcaatcgct tctgattgtt
     4681 ttcgtcctct gggtctacac caaaaaaggc aagggttttt ttaatctcgt taaggtcagt
     4741 ggaacgaaag gcaaatttta agccgaggtt atttttcagt ttttcatcta agaggtcatc
     4801 tgtattttgg gtcacgaaat ataccccagc gttcatagca cgaccagcac gaaccagctt
     4861 catagatagt gttttccctt gtgccacctg taagaagctc catgcttcgt ctaagtctac
     4921 aatcttgaaa atacttcggt ctgtatggat aaagtccaac gcaaaggtac taatgacaat
     4981 cagcattgct accgatagta actccattgt ggtatattcc tcaaaggaag tttccttgtc
     5041 aggaagtacc aagtcggcaa cctgtataat gttcagttgt ttttcaaggc tgatagactg
     5101 ctctacataa ccatcactaa aaagcagatg ggcaaagtca tagtctgtaa aactttcgat
     5161 atggtcggct atgctggtac ttagtggcgt attctcaacc cgtaattctt caatcacttt
     5221 catcaaccct cgcacttcac tattggttac tgcacgaatg gcttttctaa ggattgggaa
     5281 gcgttcccca tcacgagagg aaatccccgt aaggaatgtc aaaatatcaa tagccagtga
     5341 ttcagaatct ttggtatttt tcataattac ataagggtca agtaagcctt tgtttttctc
     5401 atcagaagtc agattgacga tattgatttc atgggaaatc tctggcaagg tttctttcca
     5461 tctgccacgt tctgcttttg ggtctacaat cactgcttgt gccccataaa gcactgcata
     5521 atagacgata aggttattcg caaaggattt accaccaccc agcgaaccga caaaggcaga
     5581 cgctaacgca ttggttactg aacccttaac cccttgactg gcaagagcag gtttcagata
     5641 gacattgcgt ccagtatcta agctgtagcc aacataaatc ccctcatttt cacccagcat
     5701 ttgagtagca ccaaaaccta aaccagcgag gaaatcagag gtcacgtatt gaatatagtc
     5761 attcatatat cttttgctgg caggtaaaaa ttcttcatgt aagcccagca tatccccaaa
     5821 aggtcgtacc agtttcacgc tcaaatcatc atagaaatct ttcacttcat tacaacgacg
     5881 tttaagttcg tcaaggtcat ttgctgatac ccttacaaca taggacagct tatacataga
     5941 ttccttgctt tggtctaaat tggtttccag ctcgttcaca ctttccagag cttccgccac
     6001 attagagctg gtttcattat cactttgcca agcgtggtta tccaagtctt tcagttcttt
     6061 ctttttattg cggacagtag atagggcttt acgattcgct acaatttcca cattcattga
     6121 cgtatcaatc ggaaaggtaa attgctgttg ctggtagtag aagatttcag aggacgggaa
     6181 gtccagttcg ccgacaatac tgttgatggt aaagtaagct acataggcgg tttcgtcttc
     6241 ctgctggatt ttcaaatatc gctgtttttc ttccaccaga cagcgagtag gcttaatgag
     6301 gtcataggtt ttaatcagtg tttcatgttc cagctttttc tttgatagag gatactcata
     6361 ctcttcatag gcagtgcctg tctgtccgta aaggtgttca atcagatagc cgaagtcatc
     6421 cttatctaat cttcggattt tgaaacgacg ggagatttta ttttctaaaa gcttttccat
     6481 cttctgaaaa cgcaggattt catcattact catactaaca aaatcgccca tcagcttatg
     6541 gttcacatca tagacaaaat cagtaaaagc attttttgct tcaacggtaa gacttttcat
     6601 agaaaactcc tgatcgttga gaagcaactt aaaaccgata aagaaacggt agtccacttg
     6661 attttcgcca atcatggata ttaaagcgtc tgtctgttgg tcgattttgt cataggcaac
     6721 cgttttgagc tttccagtga cttcattttt ggaacgttct tgtgcagaaa gtatgctgga
     6781 ttctgtactg atttgtaaag catgaatctt gccatcacga ttttgtgcga taagctgtct
     6841 gaaagaatca tgtacttgta ttttctgttc cggacttaga aatgagtaat tgtagggaac
     6901 aagctcatag taagcgtaac attccccatc tttattccag accagattgt tttcaatata
     6961 tttaattgga tatgccataa aattcactcc taactgctgt aatggcttct tgtggctggt
     7021 ttctgccaag cgttactttt tttcctgcat aggtcagctt tcgtcgcagt gcataagcaa
     7081 tgacagactt caaaaatcca taaggctttt taccatcaaa ggtttttgta gacataaacc
     7141 atgtgaaagc cacaggaatc ccaaagtatt tgagaaatgc tccctctatc atagaaagag
     7201 ggggcaagtt gccaagtatc ataactgcaa agagtgacac gacaaaccat gtcatttgcg
     7261 taaaggttat gggaaacgga agtctaaagt cattgatgga gtacaacact ttctcaacag
     7321 accagatact ggtatagctt cgtattttct tcatgtaatc aatcctttca taaaaatggg
     7381 gatagtcttt tgactacccc gtaaacaaga aaatctgcca gtaagaatgt accgacagat
     7441 ttaatagacg atttcaaaaa tcccgtgatt ggttgagata aacgttcccg aaaggtctaa
     7501 atcccgacca taggattcat aatcaatata gttttgaaga ctggctggca cttcgcctaa
     7561 agcacccgtt tcttcaatgt agtagcgtgc cacgtcagac atatcatcac aatcggaatg
     7621 aatgataata tcttcttgat gttcgcttag ttcttcaata cttgaaaaat gagtgagcag
     7681 agcagatagc tccgattgta gttcttctgg caactccgat accatttccc ataggcgatt
     7741 gagttcgcta atagaagtgt attcgtcaac cgtaaagggt aactcgtaat catgaatggc
     7801 gtattcctca tattcatcat tcaagccgat tttctctttg acttcctcaa agtcaatggg
     7861 aaaggtaaac cacgcaccga ccaattcgcc ctcattgtat ttgcctaaat tcgcaatata
     7921 gacttgcata tcgtccatat attcacgtcc tttctttgta gagattcaaa aatccctacc
     7981 gcacttcgtt tggtgtacca ttcctttgcg gaacataaga aaaccactta tattccacaa
     8041 aagaacggtt ttatttaagc accaataatg cgattgaata gctctagtaa aatgtctttt
     8101 actccagcag cgttgaagac taagccaacc gcaataatcg caataattaa aaagccaatc
     8161 agtttgctaa actcacgctt gaagccaaga tacaagccaa tcacaacgat tgctaaaagc
     8221 accagtgatt gagcgtttga tagaaaccag ttatatatgg ataggtaagt tcctgatatt
     8281 ataaatatct caaacttttc cccccattca cacggagcgt gcgactttca ccgcactccg
     8341 cgttccatcg tgtttatatt gaaaagtatt tatgtagtag acctatatca agtttaattc
     8401 ttgattgcca gctaatactc tatatttatt tagtcttatg agctggactt tatttagctt
     8461 caatagagct atatattttt gtattgtttc ttctgtttta gcgtgtatta gacggtgtac
     8521 cgcctctttt accaaaataa gatttttata atcgtcattt ccaccagctc cagttggtac
     8581 aatatgatga caatgtattt cattagcttg ttgaaattct tccagagtta tagcacattt
     8641 tccatgttga gcagaaaata gcgaaattcg attgtctgcg tattctattg tgcgattttc
     8701 caaagataat ttcattaact ggtacatcag taatttatca acacttagat tggtgtggat
     8761 aaattttctt ccctctagag tgtagcaatt cgctaaatac acctttgcca ttggttttct
     8821 gtgttggata tatccaacag ggaaaatagg ttcgccctcg ccagcaacat accgaagcat
     8881 tttagattta ccatatcttg ttgcctcctt tgtcgtaagt tgtctgcctg tttttctcaa
     8941 tctgcccctt ttgtttgtac ctaatctgtt agtgaagata gccattacag ctctattcag
     9001 tttcatacaa tccagattta cattcgttgc aatctgataa taattttgca tacctaatac
     9061 cattgaatta taaagtcttg tttcttctaa ttccgttttc ccttttgagg gagaagcaat
     9121 tttctttgcc tgtgatacca gcttgtcttt ttggtttttg aattgtttat cacttatatg
     9181 tgatttaaca actaacttct ttcttctcgg tatcatttta atcttgaaac caagaaagtc
     9241 ggagtatctt tttctaacat ttacaattct tgttttttct tgtgatactt ccaattttaa
     9301 tcgttgttca atccattgag tgattgctat tttggctttt tcagcacttt ccttgtagcg
     9361 gcagaaaact ctaaaatcat ctgcataacg aaccatatac atttccttta actttgtttt
     9421 cttcatttct ttgtaagcgt tgcttttaca tgggctaccg cttttattga tatgaaccac
     9481 atagtttttt gtaacaggat tttcctgcca ttggctttct acccaatggt ctaactcatt
     9541 aagaacaatg tttgctagta gtggggatat tattccccct tgcggagttc ctttctctgg
     9601 atatgttatt gtaccgttag tcattttgat tggagctttt aatatccttt ttaaaatgaa
     9661 tatcaagtgc ttgtcccgta ttcccatagc ccatatctgt tttatcagct tggaatggtt
     9721 cacgttgtca aagaaaccct taatatcaaa ttcaatcaca taatgtagct ggctgatttg
     9781 taggcggtta taggttgctt ttattgcatt ttcaactgac ctattaggtc taaaaccata
     9841 gctgttttca ctaaatttag cttcgcagat tggttctaat acttgtttta tacattgttg
     9901 tataagcctg tcccacatac aaggaatacc tagaggtctg gttttaccat ttggttttgg
     9961 tatttctttt cgcctcactg gcttaggtcg atacccatgt ggactgcctg caacaatgta
    10021 tcttactttt ttgacaactg tttctgctgg caatttgcca atatctttaa ttattgtacc
    10081 gtctgttcct gctgttttac tgcctgcatt tgctttgata tttcgatatg ccagtaatat
    10141 attttctctc gataggataa tatccataag atgagtgaaa atatctccat tcttactttt
    10201 ggcatataaa tcatcaaagg tgttttgcat ttcgtaatat tcagcgtgtc ttaaatcttc
    10261 tacacacaat gtttcatttt tatttttagg cataaggctt cactccttta cttgaaattg
    10321 agtgagcttt tttatcttac tcgtaatcct tattgaaaca acataatact tttctaatat
    10381 atacacgact tgagggcatt cctccattct cattacaaga atttcttagg ttcgccctct
    10441 gcttttcagc attagttcaa ctgcttattg ttcttcgtca gtttattctc ttgatgagaa
    10501 cccaatacct ttcctcgttc cgataatctt atctatacat atatatcctt aggtcactag
    10561 ctataagcct gctggcttga taccgcctgt aacgttatat ggcatttcat aacctatatt
    10621 cttactttgc cacaccagct acgatttctc gtacctttgc atttctacaa agttcaaatt
    10681 tagacccgta cattcgctgt ttcgtcagtt ccttatagaa cattctaacc atagatattt
    10741 tatagacccc cgacctgttg gatacgctac ccacctctgg atagttttcg atagaagata
    10801 tatgttcatc aactattacg gtatctttca gccgtcttta ccgagcttca aacattctga
    10861 gtttcctcat acaatgcttg tcggaggctc agggcaggcg tttcagagcg ttacttcatc
    10921 attccacctg tcagattatc agttctccta ataaccaaac tttttcgttc ttttattagt
    10981 gccttagctt ttcactaagg aacgagtcgc acaaaggttt tgtccaaaat tcataaaaat
    11041 gttctcctct ctatattcaa tgaatttgta tttgagttat ttttttgttg ttatcacgtc
    11101 ctgttctttt actgactgtt gcttcaaaat ctgcttgtgt cggtctgtca gtttcgcatg
    11161 gtcgagaatg tcttttacaa cctgcgtctg gttgatttca tcaagtttaa tcgcaacctt
    11221 taaggtcggg gcaacttgat gagatagcca gttcagcgtc ctttggaagg agtaaggctc
    11281 tggttttgtg gttagtttta atcgttcacg attgttccca ataaaccaag cccattcttc
    11341 attcagtttc caatcagaac gaggtttgga atcgtcttta tctacaaaac ggatataccg
    11401 attgataatt ttaaaggcgg tatgctctgg attgtcatag acgagtaaat cacggactgc
    11461 ataataggca cgctcatttt tcaatcgaat ctcaaaacgg ttttttactt ctgcgtcttc
    11521 aatgggaata tcatttttct tgtactgctc gtagtccttt tcatagatac agaaataaac
    11581 ttcactttgt aatgaaccga tatagagggt gtttcccata cattcctttt cctctttgcg
    11641 taccagttcg ccactgcgat agcttttaaa actgcggaag acggagatac attcttcctg
    11701 ttggcacttt tcagtgagta cagggatatt taaaatccct gtcttatcgt taatggcaag
    11761 gtcaaggcgt ttcatcacac cgccagccac caaaacgtcc ataaagaact cataccagct
    11821 tctttgttgt gccagaagat agctttcaaa ttgtctgcac ccacgacctt tcaattccac
    11881 cagaactcct ttgtccagtt catgggagca aaggacgaat atgtcgccta aagcataatg
    11941 ctctgaataa gaatagaaac catagtcctc atgaagaaaa taggacagtt tcagttgtaa
    12001 gatgttttcg accacctgct gtacgtctgt tgtcggaaag cgaattctta cataatcaaa
    12061 cagcatttca aggggagcgt cgggattgaa gcgttccaga gcttcccaaa gggactgctg
    12121 taaatcctct gatggcttga cttttcctgt ttcaatatcg cttagatact gccttgtaat
    12181 accagtcgca acagctaaac ggttttgaga tagtccataa gccaagcgtt tttcttttaa
    12241 atgctgtaac caagtttgtt cattcagtaa aaatccctcc aatcaaaaag gcgtatgtca
    12301 acttttaaag cccatttgac atacgctgaa attttgtaaa tcccttgtaa ccaaaggatt
    12361 ttctaatgtt tttttgactg tttcctgtcg atttgtaccc ccctgttaga taccgagggg
    12421 ttaagtgctg gcgtggctat tgccacacca gccagcaaga tcagtccaca cctgcgactt
    12481 ccgcttcgca cgtcgcctgc gtggactgtc tgctgttgga taacttttta atttcctcca
    12541 agaaatcata tccttttggt acaaggggag tataaaactc tgatatgaca cttgttccta
    12601 catcaacata gccacgacct ttgattcgct ttaagaagaa atccttttgt acgtcactgc
    12661 caaacatcat gccatagccc atttcagaca tacgacctaa agccactctg aaattaaact
    12721 gatcacggat tccgtcgcct aaatattttg cgtctggacg ttgacaagcc agtattagaa
    12781 agaagccagc ttgacgacct aacatgacaa tctgtttcag cttattcata actgcggtgt
    12841 tttcttttgt tcccagcatt tccatgaaag cgacgtattc atcaaagatt aagaagtgtg
    12901 ccgggagacc taagtaagca taatttttgc cagtcttata gttcttcatc tgcttcattt
    12961 cctcactacg tttcatcatt tcttcataga atgtttcaat gcaagaaagc aagtcttctt
    13021 ttctatagta gacatttgcc atcacagaac ctaagtccgc aaggtcagca tttttcgggt
    13081 caagaatata cagttttgaa tctgtatgaa gcaaggcttc aatcagtgtc agtataaagt
    13141 aagttttacc gccacctgta ccaccagcaa tcaacatatg agggagctta tcatattccc
    13201 accatacgtt tttcattaag cgaagtttac catctttagc ttctacttca tcaatagaaa
    13261 tacgactggc tatggtgtca tagagcaaag tatattccac ataggaatcc tttaactctt
    13321 tatccgtcag ctcacagtac aagccactct ctaatttctt ttccaagtgt aagagttggt
    13381 cttgatattt tcccagcgtg atttccaccc gtatctgtat caagccattt ttaagtcgat
    13441 aatacatttt agggaagtag gttatctttt cctttgtacg accagcacta tctttaaaga
    13501 aaccctctgt tttgacctgt tcagattcat accacttgtt ttcaagtatc atctttgcca
    13561 gtttttgacg gtggtaaagt tgtttaaccg tatcatagcg aacccgtttg aatacaaacg
    13621 ctaccagcaa gcagataaga attgcgacac tgaaactgat aattaaatag ggaatgtcaa
    13681 tcttatctgc ttgtgatagg ttaaaatcct gccagttgat ctgctggatt gtcttcacat
    13741 gaaacagtcc gacaaccagc aggaaaacag gcaggagtga cgctatcgta aaatgaaaga
    13801 ctaaatcttt accagatggg cgaatccttt taccacgctg tttcatgcga aaaagtctcc
    13861 tttctaccta gcgactattt gtcttgtgtc ggttctttct ttgcttgtgg ttgagctttg
    13921 aatgaactag aatcctttgt cagcacaata tcgtctgcct tgatatacca gtcaacatct
    13981 gctccttgat aggtggcagt agcaacggtg tccgcaatgg gattgataag ttccacccgt
    14041 gcgttataat caaactcttt caaaggcacg ctggcaggaa tacttacttg aatcatgcgt
    14101 ccttgtcctt tggattttaa gtcataggta cgttccttga tttcatctga aaccgacccg
    14161 tcttcatttt ggattctcac ttcacgacgt agagcagaga atttcaattc tccaaaagtc
    14221 gtgtctttat ctaatacaat gccatttgct aatctcatca tttttcctct ctttctttat
    14281 tcttttatca tgtcgtcagc atgtaaaagg taatttgtaa aaccacgagt gccgattttg
    14341 tagccctctg cggtaatacg tggattgact aacttcacac gttcctcaaa gccgaaatgt
    14401 ttttcgccag cttcagcagg aagcaccacc acaatatcat ctgctctttg aacatcagaa
    14461 tagagattat agcttcttga taagacagtt agccgtccgt tgattcttcg ctgaacgact
    14521 ttatcctcgc cagcaaattc taaattgccg aatgtttttt ccatgttggg aatcacaaat
    14581 ttaagttcca tatttttacc tatcctttct tttttattgg ctgaatgaat gtttgatggt
    14641 cttaaagagt ggggaacgac cttttgattc ttgatttttt gttttcataa gttcacttcc
    14701 tttcaaaatc gggtaaaaaa atagacacct cattttttga agtgtctacc tattaaatat
    14761 tcaaatttta ttggaagtat ctttatatct tcacttttca aggataaatc gtcgtatcaa
    14821 agctcattca taagtagtaa attagtagta aattgagtgg ttttgacctt gataaagtgt
    14881 gataagtcca gtttttatgc ggataactag atttttatgc tatttttctc ttaaaaaata
    14941 tcattatttg tggtatggag cccccgcgtt gatccggaag gcacggtaga tttgctcgac
    15001 gaggatcagg cgcatcaatt gatgtggata cgtcattttg ccaaaagaga tctgagcatt
    15061 gcttcttttc atgacggctt cgctgagtcc taaagaaccg ccgataacaa aaacaaggtg
    15121 gctttttcct tggataccta attggtcgat ttgtttagca aaagattcgc ttgtgggatt
    15181 tttcccttca attgctaagc catagacgta ttcttgttct ttgattttcg ctaagatccg
    15241 ttctccttct ttttctttta cttgtctcat ttgcgcttcg cttaaattct ccggggcttt
    15301 ttcgtcagga acttcgatca attcgatttt cgcataggca ttcagccgtt tgacatactc
    15361 attgatacct tggatcaagt atttttcttt taattttcct actgaaataa ttttgatatt
    15421 catgttttct ctcctttgat cttcacgctc actttgttta ttataatcaa ttttttcgtc
    15481 tatatgttta ttttgactat ttttgttaaa aagatttccc caacagttat gcacaacatc
    15541 cacaggttta tccacagaaa acagcgttgt tttaccttat ttcatcacat tcattaggca
    15601 aacatacgtt ttataagaat ttatccactt tatacacagg ctgtggatat gttttatccc
    15661 ttatttttcc aatcctatcc gcgtagtttt acacaatatc tgtggaaaaa gaaaatcaaa
    15721 tattttcatc tgaaaacaat gatttttcct aaagtttttc ataaaaagtt caaagtctca
    15781 aagtagacta aagtcaacaa ataagactat actaacagta gataaatgaa tgtaattgga
    15841 ggaggaacac atggatagaa aaaatgtgac accaaagatg aaaaaaaata aaaataatag
    15901 cctctggcgt aagttgggtc tggggcttgt tggagggatc gttggaggac tactgacagc
    15961 tggcattttt tatgcagcga tgggcactgg caacaatagt tctactgcta caagcggcaa
    16021 tcaaaattct gccggtgaaa cagttgtgga aaatgtcaaa gtcaatgtag attctgatat
    16081 caccaaagcg gtagataaag ttcaagacgc tgtcgtttct gttatcaact tacaaagtca
    16141 aaaccaatca agcggttttg gcggattatt cgggcagcaa gaagaaagta gcagctcaga
    16201 tgatagtaat ttggaagctt atagtgaagg aagcggcgta atctacaaaa aagacgggaa
    16261 tactgcttat gtagtcacaa acaatcacgt agtagatgga cagcaaggtt tggaagtcat
    16321 gatgaaagac ggtacgaagg taaaagctga attagttgga acagatgcct attcagattt
    16381 agcggtgttg aagatcaatt cagacaaagt cgaaacggtt gcctcttttg gggactcaag
    16441 tgctttgaag gttggtgaac ctgcgattgc gattggttcc ccattaggat ctgaatatgc
    16501 gaattctgtc acttcaggaa tcatttcttc cttgaaccga caagtaacaa gtacgaatga
    16561 atcgaacgaa acagtcaata tcaatgcgat ccaaacagat gcagcgatca acccgggaaa
    16621 ttctggtggt cctttagtga atatcgaagg acaagttatc ggtatcaact caagtaagat
    16681 cgcaagtact tctgaatcga cttctaatgt aagcgtagaa ggaatgggat ttgccatccc
    16741 aagtaacgat gtagtgaata tcatcaacca actggaaaaa gacggaaaag tgacacgtcc
    16801 agctctaggt atcacaatgg tcgacttatc tgccgtttct actcagcaac aagaacaaat
    16861 cttgaagatt ccttcatctg taactaacgg tgtcatcgtg acaaatgtcc aaacagctac
    16921 accggcagaa aaagctgggt tgaaacaata cgacgtcatt actaagatcg atgacaaaga
    16981 agtttcttct ggtgtagaac ttcaatctgc tttgtataag aaaaaagtgg gagattctgt
    17041 caaaatcact tactatgaag gctcgaaaga aaaaacaact actgttgaat tgacgatcga
    17101 tcaatcggca ttaaaacaaa atcaaaatag tgaaaattaa taaaaaagag gttgtgacaa
    17161 agacttgtca caacctcttt attttggaaa tacttctccg actacggcat cctcaggcaa
    17221 tactaagatt cctttttttt ctggagcatt tggcaaatgc aattcacgag cagaacaaat
    17281 cattccatag ctggctaccc ctcgcaaagt tcctggccag atcatcattc catctggcat
    17341 catggctcct ggttttgcaa cgacaacttt ttgtccagct tcgatatttg gtgcaccgca
    17401 aacaatttgc aatactgtcc cattgtccac ttctgtctca gtaatcgata aatggtcaga
    17461 gtcaggatgc gggacgcatg tttttacata cccgacaacg atcttcggtt catgatcagc
    17521 ttcaagctgt tcttcgaatc ctgcttcttt aatcaatcga ttcaatttcg ctaattcttc
    17581 atctgttaag cttacttggc cgttcccttc aattgttaga tgatcggaga tatggaaaaa
    17641 gttccaagca acaacttgac cttgttcatc tttgatccga gcaacattcc atttacgttc
    17701 gacagtgttt tctgttcctt tatcgtcggc tatgataacc atcaaagtgt ctccgacgtg
    17761 tgctttgtta taagcaaaaa tcatgttctt tcctccatta tcctttgctg agatttatct
    17821 taacaaaaaa ttgcattcac tgcatgagat ttttttcatg ctttatttcc gaagaagctt
    17881 cttctggaac accatttatt cgtaacaagg gactgtgaca agacttttgt cacagtccct
    17941 ttactgtttg attaaaaagg ttatacaagt tccacttgct taattactcg gctgttcttc
    18001 ttcgtcagaa acgatcaaga catcacaagg tgaatgacgt atgacgtagc tggctacact
    18061 tcctgtcata aagcgttcga ctgcatttag tccagactgc ccaaccatga ttagatcgat
    18121 tgcatatttt tttggcaatt tgactgccat ggattcacga acagagccgt atgcaataat
    18181 tccttctata tttgtgtaat caacagattt tccatattct ttacattctt cgatcatttg
    18241 cttggctgtt tcagtttgct gatcgactag attttgatca ataggagcat agcccataaa
    18301 attataaaat tgctgatcga ttacgtttgc tacatatact ttcccattat tacggcgagc
    18361 cacttctaac gcttttttga aagcagcaag tgcttgttca ctaccgtcaa tacctactaa
    18421 aatgttctta tattcttgat tcatcttgac ccctcctaac agatgtcagc cttttattac
    18481 actttctagg aacgattcga tttcctgttt tgttttacga tctttattca ctagacgacc
    18541 taattctttt ccgtcttgga tcacgacaaa acttgggata ccaaaaatgt tccattcagc
    18601 ggctacatct atgtacttgt cacggtctac ttcaataaat tgccaatcag aaaagtcatt
    18661 ttcgatttca ggcatttgcg gtttgataaa acggcagtct ccgcaccagc cggcagtaaa
    18721 gaaaaataca ctttttccag tttctacgta gcctgctaat tcttctaaac tatttgggat
    18781 aatcattctt tttcctctac tttctgttta tgtatttttc aattaaatta tacacctttc
    18841 tttcgaacta acaaacattg atcctctttt tataaaaaaa atctgacaaa aggttgcttt
    18901 tgccagattt tatagtttca ctatcgcgtt atttcccgta tacgatcgtg ttcactgttg
    18961 atttatctaa acctttcaat gtttgaatca acatttctcg tgcagcttca aaatcgcgaa
    19021 tactgaacat cgtttgatgc gtatggatat aacgtcccac tacgccaatc actgtacttg
    19081 ggattccttc attttgtgta tgagcagcac ctgcatctgt accgcctttt gaaacaaagt
    19141 attgataagg gatgttgttt gtttcagcga tatctagcaa gtactcacgc aaacgaggaa
    19201 gcatgatcaa acctggatct tggatacgca tcaatgtacc ttctcctaaa tgtccgaatg
    19261 tgccattttt cgtcactgtg tcatctgctg cagagcagtc tactgcaaag aacagatctg
    19321 gcttgaattt attgacagaa gctttagaac cacgcaagcc tacttcttct tggacattcg
    19381 cgcctgcaat caatgtatga cctaatgttt cattttgtaa cgcttctaaa gcttctaata
    19441 ccattgtaca gccatagcgg ttgtcccacg atttgctgat gatattcttc ccgttggctg
    19501 ttttgatcgt ttcagcgtaa ggaacgattg tgtctcctgg aagaacaccg taactcattg
    19561 cctcttctct tgattcaaac ccagcatcga acaaaatatc tgttacttct actgattttt
    19621 gtccgcttgt tcctcgtaat aaatgtgggg gaacggaaga agaaatacat ggataattct
    19681 taccagtaga ggttttaagt gtaaaacgtt gagcagaaac aacgtaaggg ttccatccgc
    19741 ctagaggtac gactttaaaa agcccgtttt cttggatttg tgtcagcatg aatccgactt
    19801 catccatatg tgctgcgacc attacacgag gtgcatccgc ttcttcatgg tggcgcagcc
    19861 caaaaatgcc gcccaaacca tctaactgta actcatctac cagcggttcc atatgacttt
    19921 ccatataggc gcgaacgtct tgttcaaaac cgcttgttcc ttgtaattct gttaactctt
    19981 tgattcgttg aaatgttttt tcttccatat gctattcgtc taaatttcag actaaactcc
    20041 tctcagactt atattgatct gcggataaaa tatgattttt tctgcagacc gatcgtattt
    20101 attatagctt attttttctg gtagcggtta tttatttcaa agaaaatatg agagtgtggt
    20161 aaaataaaca caaagagtag caagtgagaa gaaggaggta tatccacatg tatgaagaaa
    20221 gcgaattaag ctattttaaa ggcgggttag ccgttgggat cggtctagga ttattgagtg
    20281 gggtagcttc aacgatttgg tatcatcgca aaaaagtgtt atcagcagac caagtgcttg
    20341 aaaatatcaa aacatctttt ttaaaagaag gcgcaatcga aggtgcttgg atctcttttg
    20401 aaaaagaacc tattcggaaa ttcgccgttc ggtcgagtgc ttatcggggt gggctttccc
    20461 ggatagaaga tggcgaaact gtttattatg aatttttagc agatgctcat acaggaaccg
    20521 tattagacat ttcaagaaaa aagggcgcaa tagattaata cgtcaaaaaa atgaaataac
    20581 gaatacgcac ccactatcta aaagagaggt atcctatcct tcttttatag tgggtgattt
    20641 ttataaaaat agggctattt cctcttggaa tagctagtat ttgtagttat tatacggtat
    20701 tcttgaaata agattagttt tattatataa ggagagtgac aaatggaatc acgtgaaaaa
    20761 attgccgtgc tcattccttg ttataacgaa gaggcgacaa tttcaactgt tattgaagat
    20821 tttaaaaggg aattaccaga agcagatatt tatgtctatg ataataattc gaccgatcga
    20881 acatacgaat tggccgttca aggcggagca atcgtaaaga aagaaccacg tcaaggaaaa
    20941 gggaatgtaa tccgtcaaat gttttttgat attgatgccg attattattt gatggtagat
    21001 ggtgatgata cttatcctgc agaagctgca catggtttgc ttgagaaatt acgttctggc
    21061 gaagcagata tggtcattgg cgatcgtcta tcaaatggaa cctattttga tgaaaataag
    21121 cgtcctttcc atgattttgg gaataatctg gttaaaaata caattactta cttatataaa
    21181 acaaaaattc gcgatgtcat gaccggctat cgaggattca atcgaatttt tgtcaaaagc
    21241 ttccctatta tgagttctgg ttttcaaatc gaaacagaat tgacgattca tgctttagat
    21301 aaaaaattca agttcgttga gattccaatc gattatcgcg atcgtcctga aggtagcgaa
    21361 tcaaaattga atacattctc tgacggattc aaagtgatca tgatgatcat caaaatgtgc
    21421 aaagattata aacctttgct tttctttggg atctggacca tcttcttctt tttatttgga
    21481 ttggtcatgg ggattcctgt gattcgtgaa tatatgctga caagctttat tacacgcatc
    21541 ccttctgcga ttttaagtac tggattgatg actttagccc ttctttcatt agtaacaggg
    21601 ttgattttgg atacggtcgt cacaaatgcc aagaaagaat atgaattgaa tttataccat
    21661 gtctaccacg aatatcaaaa taaagaaatg aagtaggagg ccttttttat gcataagaaa
    21721 aaggagaatc tatttcctac tttttttgca actttatttg cgtcaggttt tgcttttttt
    21781 acttcttatg gtctaggaac tttgaatcca acagtaatca ttggtttagc catggttttg
    21841 ctacttgctt ttaagcaata tggaatagag ttgttttcaa taaaaaacaa agttgcagct
    21901 attgcccgat tgtttttttc tgcagctttt gctcttacac tttctattgg cagacagata
    21961 caatttcagt atgtctcagc aaactataca aagacaacga ttgctgatgg aagtattttt
    22021 tactttcttg tttatttctc catttttgtg tattcttttt tatttttaca atcgatttat
    22081 ttatcaatgc caaaactgga aacatttttt actgctcaaa acacacaggc tttctcttca
    22141 agaaaatatt ttttattctt tactgtcttt ttagttctgt gttggcttcc ttatctgttg
    22201 gcattgtatc ctggagtggt tttgcctgat tcgttaagtt cggtcgccca atctatgggt
    22261 gatgttccta taagcaatca tcatccagtc ttattcacct tactggtcaa gctatttgtt
    22321 catgatttac caactgatac gatcaatcac ggtgtatttt tattttcgat ttttcaaaca
    22381 ttgataacag cttcagcgat aagttattct ctttgctggc tgttaaaaag aaacatccat
    22441 ttttttccgg tactcattag tttatgctat tttgcatttg caccggtttt tccaatctac
    22501 gccttgaaca tgcaaaaaga tactttgttc tctatcgctt gctttttatt ttccttagcg
    22561 gtttttgact ttatttttca acgagaaaag ccaacgattc tcaatcagat ttacgtaatc
    22621 atccttgctt tactgaccac ctatctaaga aataatggga tttatgtagt aaccggcaca
    22681 ggtttgattt gcggcttgat tattttgaaa caaaaaaggt atcaagcatt ctttcaagtg
    22741 gtgctgggtt atacagtgat tgctggacta ctcatccacc cgttgatggc tcgttatgct
    22801 attcctacag aaactgctga acaattaggt gttcctttgc agcaaatttc tagaactatt
    22861 gttatggatg gtgacttgtc taagaaagat aaagcattca tgaatcagct actcccgata
    22921 aaagattatg aagcgtattc tcctagtctt gcagactcaa tcaagtggca taaaaatttt
    22981 aacaatgagt ttttgaatac acataaaaaa gaatttctat cactatgggc aagaaattta
    23041 ccaagtaata taaaaatcta tacagatgct tatattttag aaacgttcgg gttctgggta
    23101 cctggtacaa aaaatagtta cggcttttta gatactcgtg taaacacgaa taattatggt
    23161 atgaagcaag tagatttgat tgaacatctt actggattgt caggaatgaa aaaaatcatc
    23221 gatgctcgtg atttctttgg ttccggcaca ttactgtggt tagctatcgg cagtgcactt
    23281 ttagcaatct ttaagaaaaa atatcggtat ttactccttt ttcttcccgg tatgcttgtt
    23341 tttgctacta ttatgattgc tactcctgtg gcttttagtt tacggtatgt gtttgtttta
    23401 gcattaggtc tacctgttta tatcctctct ccttttttac tagataaaag agaaaaaatg
    23461 aaaacagata taggagatac tcatgaaact atttaaaaag atattttttg cttttgttct
    23521 cttgatcgct agctattgcg tactactgac aattactttt ttcattccgc agcactctat
    23581 tgaatcgaat gcagaaaaat ctctacagat ggttgaaaaa gaaggaatgt atccttctat
    23641 caatcaagga aatatgctag gcactcggtt agataatttt actgatcatt tgatgatacg
    23701 aaaaacaaaa gcagatcctg aattgaactc cttggagaac gcgatgtcta tggcggatta
    23761 tccccgttat tggcatggtt atcagctttt tttacgacct ttactgctag ggatgagttt
    23821 ggggagtatt cgaatgattt acgcagccat tctttttctg ctgattggat tgacgagcta
    23881 ctttttaacc aagtgtagcg acatctattt aacgacagcc ttgttgattt cgcttgctat
    23941 aggaaatgct gcaacctttt tcttcagtat gcaattttca aatatttgga ttttgacttt
    24001 attgtctgta ttactatttt taacaaaacc acaactctcc aacaccaacc aacaattatt
    24061 cttgtatttc tttgtcattg gttctttagc taactttttt gacttgttga caacaccggt
    24121 catctcttgg gggatacctg tgatcattat ttattatgta acaaataagt atttaatgga
    24181 taaacgcagt tcattgagca aacaagtagg cagttttgtt tttaccggta tcttttgggg
    24241 attaggctat ggtttgacat gggtggctaa atggatccta tcatcgatca tattgaacaa
    24301 aaacattgta aaagatgcaa tcaaccaaat cctctttcga acggaaggaa acgacaaata
    24361 cccgttgcat cgattagaga tgctgaaaag caatattaga ttgatgtatc caaaagtagc
    24421 aatattattg ctattgatta cttgtcttgt ttttctctat tttgcttatc gtaaacgaaa
    24481 tagctggcgt ccgttccaac ttctagtttt gtttgtttta gctgctgtaa cgccgtatat
    24541 ttggtacaat attttagcta atcactcaca gattcattat tggtttacgt atcgtacgca
    24601 aatcatcaca agttttgcta tacttagtgc tttcgccttt ttgatccctc ctgagaaatc
    24661 aaaagacgaa ctaaattact tttaagaata ccgatgtact aattcatttt taaacataaa
    24721 aactccagct gtcagagata gccggag
//
LOCUS       DAZUMM010000038        24271 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000038 DAZUMM010000000
VERSION     DAZUMM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.038"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..572
                     /locus_tag="ACG8E6_001459"
     CDS             <1..572
                     /locus_tag="ACG8E6_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFS5061879.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="ACG8E6_001460"
     CDS             576..1217
                     /locus_tag="ACG8E6_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5061880.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="ACG8E6_001461"
     CDS             1214..2131
                     /locus_tag="ACG8E6_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5061881.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="ACG8E6_001462"
     CDS             2128..2799
                     /locus_tag="ACG8E6_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5061882.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="ACG8E6_001463"
     CDS             complement(3340..3828)
                     /locus_tag="ACG8E6_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFS5061883.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="ACG8E6_001464"
     CDS             complement(4029..4295)
                     /locus_tag="ACG8E6_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS5061884.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="ACG8E6_001465"
     CDS             complement(4295..4558)
                     /locus_tag="ACG8E6_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5061885.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5079..5219
                     /locus_tag="ACG8E6_001466"
                     /pseudo
     CDS             5079..5219
                     /locus_tag="ACG8E6_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5361..7184)
                     /locus_tag="ACG8E6_001467"
     CDS             complement(5361..7184)
                     /locus_tag="ACG8E6_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5061886.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="ACG8E6_001468"
     CDS             complement(7782..10487)
                     /locus_tag="ACG8E6_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFS5061887.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="ACG8E6_001469"
     CDS             complement(10793..11740)
                     /locus_tag="ACG8E6_001469"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFS5061888.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="ACG8E6_001470"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="ACG8E6_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS5061889.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="ACG8E6_001471"
     CDS             complement(12375..12737)
                     /locus_tag="ACG8E6_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5061890.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /gene="alr"
                     /locus_tag="ACG8E6_001472"
     CDS             complement(12748..13869)
                     /gene="alr"
                     /locus_tag="ACG8E6_001472"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFS5061891.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /gene="acpS"
                     /locus_tag="ACG8E6_001473"
     CDS             complement(13885..14235)
                     /gene="acpS"
                     /locus_tag="ACG8E6_001473"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFS5061892.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /locus_tag="ACG8E6_001474"
     CDS             complement(14365..15876)
                     /locus_tag="ACG8E6_001474"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS5061893.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /gene="murF"
                     /locus_tag="ACG8E6_001475"
     CDS             complement(16198..17550)
                     /gene="murF"
                     /locus_tag="ACG8E6_001475"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFS5061894.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="ACG8E6_001476"
     CDS             complement(17703..18779)
                     /locus_tag="ACG8E6_001476"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFS5061895.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="ACG8E6_001477"
     CDS             18975..20720
                     /locus_tag="ACG8E6_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFS5061896.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="ACG8E6_001478"
     CDS             complement(21000..21437)
                     /locus_tag="ACG8E6_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFS5061897.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="ACG8E6_001479"
     CDS             complement(21421..22794)
                     /locus_tag="ACG8E6_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061898.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23116..23604)
                     /locus_tag="ACG8E6_001480"
     CDS             complement(23116..23604)
                     /locus_tag="ACG8E6_001480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5061899.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23626..24228)
                     /locus_tag="ACG8E6_001481"
     CDS             complement(23626..24228)
                     /locus_tag="ACG8E6_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS5061900.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6959 a         5163 c         4488 g         7661 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agctcaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgtc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcgt aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctg caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    22861 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    22921 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    22981 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    23041 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta
    23101 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    23161 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    23221 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    23281 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    23341 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    23401 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    23461 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    23521 cggcacaaaa gcttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    23581 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    23641 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc atggtttcaa
    23701 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    23761 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc
    23821 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    23881 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    23941 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    24001 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    24061 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    24121 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    24181 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    24241 catctttttt catttattaa aactgcctcc t
//
LOCUS       DAZUMM010000039        24270 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000039 DAZUMM010000000
VERSION     DAZUMM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.039"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..800
                     /locus_tag="ACG8E6_001482"
     CDS             <1..800
                     /locus_tag="ACG8E6_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFS5061901.1"
                     /translation="LVGFVLGILVNNALADMFIGNSEWIGAIAGTVGHLAVFLLALFL
                     ASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYLTGAYRLDSGNANIV
                     PLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALFALLHGLNPNMTFLA
                     VFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVSGTQEQGFFSTLIQS
                     QNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTAVYQ"
     gene            996..1970
                     /gene="sgrA"
                     /locus_tag="ACG8E6_001483"
     CDS             996..1970
                     /gene="sgrA"
                     /locus_tag="ACG8E6_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFS5061902.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            2268..2624
                     /locus_tag="ACG8E6_001484"
     CDS             2268..2624
                     /locus_tag="ACG8E6_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061903.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            2756..3784
                     /locus_tag="ACG8E6_001485"
     CDS             2756..3784
                     /locus_tag="ACG8E6_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061904.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(3810..4250)
                     /locus_tag="ACG8E6_001486"
     CDS             complement(3810..4250)
                     /locus_tag="ACG8E6_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5061905.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            4410..4916
                     /locus_tag="ACG8E6_001487"
     CDS             4410..4916
                     /locus_tag="ACG8E6_001487"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS5061906.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            5084..6997
                     /locus_tag="ACG8E6_001488"
     CDS             5084..6997
                     /locus_tag="ACG8E6_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5061907.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            7002..8003
                     /locus_tag="ACG8E6_001489"
     CDS             7002..8003
                     /locus_tag="ACG8E6_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5061908.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(8205..8411)
                     /locus_tag="ACG8E6_001490"
     CDS             complement(8205..8411)
                     /locus_tag="ACG8E6_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFS5061909.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(9057..9905)
                     /locus_tag="ACG8E6_001491"
     CDS             complement(9057..9905)
                     /locus_tag="ACG8E6_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5061910.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            10069..10971
                     /gene="gnd"
                     /locus_tag="ACG8E6_001492"
     CDS             10069..10971
                     /gene="gnd"
                     /locus_tag="ACG8E6_001492"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFS5061911.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            10973..11974
                     /locus_tag="ACG8E6_001493"
     CDS             10973..11974
                     /locus_tag="ACG8E6_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFS5061912.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            11971..12255
                     /locus_tag="ACG8E6_001494"
     CDS             11971..12255
                     /locus_tag="ACG8E6_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFS5061913.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            12288..13085
                     /locus_tag="ACG8E6_001495"
     CDS             12288..13085
                     /locus_tag="ACG8E6_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5061914.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            13099..13932
                     /locus_tag="ACG8E6_001496"
     CDS             13099..13932
                     /locus_tag="ACG8E6_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5061915.1"
                     /translation="MEILSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            13950..14807
                     /locus_tag="ACG8E6_001497"
     CDS             13950..14807
                     /locus_tag="ACG8E6_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5061916.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            14829..15338
                     /locus_tag="ACG8E6_001498"
     CDS             14829..15338
                     /locus_tag="ACG8E6_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS5061917.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            15375..15776
                     /locus_tag="ACG8E6_001499"
     CDS             15375..15776
                     /locus_tag="ACG8E6_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061918.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            15888..16241
                     /locus_tag="ACG8E6_001500"
     CDS             15888..16241
                     /locus_tag="ACG8E6_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061919.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            16275..16718
                     /locus_tag="ACG8E6_001501"
     CDS             16275..16718
                     /locus_tag="ACG8E6_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5061920.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            16735..17820
                     /gene="uxuA"
                     /locus_tag="ACG8E6_001502"
     CDS             16735..17820
                     /gene="uxuA"
                     /locus_tag="ACG8E6_001502"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS5061921.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            17813..18526
                     /locus_tag="ACG8E6_001503"
     CDS             17813..18526
                     /locus_tag="ACG8E6_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5061922.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(18733..18882)
                     /locus_tag="ACG8E6_001504"
     CDS             complement(18733..18882)
                     /locus_tag="ACG8E6_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061923.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            19278..19478
                     /locus_tag="ACG8E6_001505"
     CDS             19278..19478
                     /locus_tag="ACG8E6_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5061924.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            19653..19775
                     /locus_tag="ACG8E6_001506"
     CDS             19653..19775
                     /locus_tag="ACG8E6_001506"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061925.1"
                     /translation="MNMNRDVLKMNEFDQSLSYRNLQFRSIGLSINEKVKKNMF"
     gene            20046..20609
                     /locus_tag="ACG8E6_001507"
     CDS             20046..20609
                     /locus_tag="ACG8E6_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061926.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(20670..21035)
                     /locus_tag="ACG8E6_001508"
     CDS             complement(20670..21035)
                     /locus_tag="ACG8E6_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFS5061927.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(21111..21356)
                     /locus_tag="ACG8E6_001509"
     CDS             complement(21111..21356)
                     /locus_tag="ACG8E6_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5061928.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(21426..21680)
                     /locus_tag="ACG8E6_001510"
     CDS             complement(21426..21680)
                     /locus_tag="ACG8E6_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061929.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(21844..22167)
                     /locus_tag="ACG8E6_001511"
     CDS             complement(21844..22167)
                     /locus_tag="ACG8E6_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061930.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            22354..22427
                     /locus_tag="ACG8E6_001512"
     tRNA            22354..22427
                     /locus_tag="ACG8E6_001512"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22388..22390,aa:Ile,seq:cat)
     gene            complement(22812..23309)
                     /locus_tag="ACG8E6_001513"
     CDS             complement(22812..23309)
                     /locus_tag="ACG8E6_001513"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFS5061931.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(23349..24158)
                     /locus_tag="ACG8E6_001514"
     CDS             complement(23349..24158)
                     /locus_tag="ACG8E6_001514"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5061932.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT         8366 a         3806 c         4951 g         7147 t
ORIGIN      
        1 tcttagttgg ttttgtttta ggaattcttg tcaataatgc acttgcggat atgtttattg
       61 gaaatagtga atggattgga gcaattgccg gtacggtcgg acatttagct gtttttttac
      121 tagctttatt tcttgctagt aaggtcgaag gaaaaagaat agaagattat ggtgtttctg
      181 ttcgaggaaa agactggggg tatttagcgg gaggtctcgt tgttggaatt ggagtgtttc
      241 tgcttattac tagtccatta tatttgacgg gggcgtatcg gttagattca ggaaacgcaa
      301 acattgtgcc gctgataacg agttttattt tatttattgc agtgggggca tcagaagagc
      361 ttttattccg tggctttttt cagcatcaga tgcttagatt tggtccgtta atagcaatga
      421 ttggatctgc tgcattattt gctcttttgc acggattgaa tcctaacatg acatttctgg
      481 cagttttcaa tatcttttta gcaggttgct ttttcagcgc acttatctac agtacaggaa
      541 gtttatttac ggctattggt gctcatatta cttggaattg gacacaagga gctattttgg
      601 gtataccagt cagcggaacg caagaacaag gttttttctc cactctgatc caatcgcaaa
      661 atacaataat tactggggga aactttgggg ctgaagcatc tatatcaact acattattct
      721 tgttagtctt gattgttggt ttgttactct atgcatataa aacaaagcgg atgaagaaat
      781 acactgctgt ttatcaataa aagatgaaga aagcaaggct gatatcggtc ttgctttttt
      841 aagtctgtca ttgattaaac gaaaaagtaa acaaattcaa gcgaatacac gacatctttt
      901 gttagatttt cacaactgac tgtcgtgttc tcatcatttt cattttatat aattccttta
      961 tcaaatagaa ggtaattata tggagagtga tagaaatgaa gaaaacagcg accgtattat
     1021 ttgttattct tgggctgagt ataattgcag caatgattgg aatcagtact tctgcaaatg
     1081 aacgggcaaa tgagaatctt tcatttactg tgaaaactga tcggattgtt tatgacatga
     1141 cacaacaagt cattactatt ccagtcaaac caaacaaaag cgtgaatgct agtgatgtac
     1201 atgctgttct aacatatggt tgggatggta atggaagttc agaaaaagtt attggggaag
     1261 tttatttaaa agacgtacaa tggaccgctg gtattgagta tacaataatg atttctgcag
     1321 aactatcgat tgatgagatc aagagtaaag ataaagttga tttgattgtt ttttatgatg
     1381 gacagatgac gatcacggag aatttgaaac caagcagttg gacagttgtt ggaccgccaa
     1441 gcagtacgga tagcacaagt agtgaaagca gtacagaaaa cacgagcggt gaaagcagta
     1501 cagagagtac gagcagtgaa agtagcacag aaaacacgag cagcgaaagc agtacggaga
     1561 gtacgagtag tgaaagtagc acaggaagca cgagcagcga aagcagtacg gagagtacga
     1621 gtagtgaaag tagcacagga agcatgagta gtgagagtag cacaggaagc acgagcagcg
     1681 aaagcagcac aggaaaagta aataatgaac ttgatttggt acctatcata ccaactaagg
     1741 aacaaaagag aaatgcaggg agcaacctaa aaagtgtgct aaaacagcct gattttgttt
     1801 ctcgtggaat tgatcatcaa gaaaaagcga aagaaacaga taatagaaaa agcgatttac
     1861 cagaaactgg tagtagaacc ttgaacaagc taattacatg gatgggtgtg ttactcattt
     1921 tgattgtagg agcaagctat tttcgttctc tcttccatcg agtgaaataa agaataaaag
     1981 caacagatct aaaattgaat aaaacgatag atttttgagt gaatatttca ttcgagagtg
     2041 ctgtattgcc tggcgaagtt gtagctttgc cagatctatt tttgtttact aacatatttg
     2101 agaaacagga aataacagga acacaaatgg aaaataagag caagaataat tgacaatgca
     2161 cactgcgcat ggtagtatct ctgaaaaatc agtaacgaat ctatttgtac gttattcatg
     2221 tagctgtcgg aataagtcta aataatagat aaaaaggagt atgtactttg tttgaagaat
     2281 ttattgatat caatgaaagg caagtatatc aatttttgaa ctattgttat gaaagagacg
     2341 agaaattata tgtggtgaag gatatcgctt tagatctaaa ctacaccctg gctaagatga
     2401 attcggtgat tcaacaagca gaatcgtttt gcgaaaggta tccagaatat aaattatctt
     2461 ttctttcaga aaacaagatg ataaaagtcg agttttcttc tcagttttta ctgagtaaag
     2521 tttattctat acttttagag ggaacgattg gttatatttt actggattca ctttacaaag
     2581 gtacttatca atccttagaa aatcttagtc aaaaaattat ataagtttgc gtacgattca
     2641 aagaaaatta agagaactta atactatttt gaaaaactat catttatctt gcagcttgaa
     2701 aaaaggtaat ccgatagaag gagaagaata ccgtatccga tacttttttc acttgatgta
     2761 ttggcagtca ttcgacgaaa atcattctga tatattatca agtataaaaa ataaacgtca
     2821 actattagaa aaatttgcga aacatgcgcc atatacgcgt agtatagata gaaagaaatg
     2881 gatcagccta ctaagaattt cgttgcaacg aatgaaacaa ggccactcga ttacgtatgt
     2941 cccagaagag atgaacaaat ttgtgcatcc attgataaca aaagaagagt ttacgcatta
     3001 catactagag cctttttttg aagcaaactt ctggtcgatg aatcacatca ctccaacaga
     3061 gcaagcttac ctttatttta tgtttggtgt aatgaacacc tatttggaag aagatttgtt
     3121 ggatactcca gataaagtac aagaaagttg gggctggatt gaatcgtcta ttgtagaaaa
     3181 ttttgaaaaa aacttcaaga tttgttttac aacttcagaa aaaaattatc tatatgtcaa
     3241 tttggcaatg atccatttgt attcaagggt ttttgggaca aaaaagaagg tggatgcttt
     3301 tggaaaatca gcagaagaca gagattttca gcatatattt ccagtgatgt atccaatcat
     3361 gcaacagttt tttagtgggg tagctcgcag aattccagag cttaatactt attatgaaaa
     3421 gaacagacgg ttgatttttc aatactgtat gttagtcagg gatattttta taaaacacga
     3481 acaacctgtc atattctata ttcaatcaaa gtatggtaaa acacaggaat tatgggcaaa
     3541 gaaaaggata ttatctatga ctgaacgccc aataaaatat agtgcatccg ctgatcagtt
     3601 acctgatatt gtcattagcg attatcctat cgacaaaaag gcatatgatc aaacgaatac
     3661 acagatattt tactggaatg gacaacctac aaaaaatgat tgggcgcgtc tgaaagctga
     3721 aatcataaaa atcaggaatg agaaaaatag atccatattg gaaaaattgg atttcgttaa
     3781 ttaagaaaag cagattgttt atgccaaaat taagacattg caaaaagatt attttcttta
     3841 aaatcgacaa gatgaccaga aagcaagagt tcgttgagct cttgctttct tttagtttta
     3901 gaaaggaaca tcctgatttt atcttcgcta aatccagtca aaagttttgc agattggtcg
     3961 tagataatcg gttttttcaa taaagaaggg gatgtacgaa tctcaatata tagttcactc
     4021 aaactgagtt catctattct gtgaacaaga tttttataac taatagaatg agtagagagc
     4081 atttcggtca cgccgttgtt tgtcaatttt agtaagtgga taaattcatt tttggttagt
     4141 ggttgacgaa ttaagttctg ttcgataaac ggtatatcat ggacgataag ccaacgtttt
     4201 gcgcgtcttg aagacgagca gccagaacct gtatacaatt taatcatcat acattcctcc
     4261 aatagctaag tatttatgaa ataataacat gaagaaatag tcgaaaagtg ccatttagag
     4321 gtgtaaaata agaaaatgtt gtcacgtata atggaactag ctagataaga aatactatta
     4381 aatcaagaaa aaagaaagaa ggcacaagta tgtcaaacac agccgacgca atagtagtga
     4441 tcgatttaca aaaaggggta tgttttgatt cttcgccgat acacgaatta gaaacagtga
     4501 cagcattagt gaataaaaga atcaaaacat acgaaacgat tgggaaacca gttattttcg
     4561 ttcaacacga agatgctcaa ttagtgccac aaacaaaacc atgggagcta ttgtcggaat
     4621 tatctattcc tgagcacagt tattttgtga gaaaaaccca tgcgaattca ttttataaga
     4681 ctaatttgag tgttttatta actgaattgg actgtcattc gatcgaatta tgtggggcac
     4741 aagcagagta ttgtatagat acgactgtga agtttgctca tggattagga taccagctta
     4801 tgatgtgtag aggagcaacg acaacttatg ataatccttt tatgaaagca aaggaaacaa
     4861 tcgcttttta cgagaaaatt tgggataaac gatttctgac atttttggag caataagttg
     4921 tttcttcgtt taaaaaaagc agaatggata ctggtttatc cattctgctt tattacgtaa
     4981 aatgagaaaa taatcctgtt ctatttttat gggataaacc atttattttt tgtataaaaa
     5041 atgtttgcgt ttacatataa ttctcctaga aacataaaaa ggagtgaata gcttgagaac
     5101 aaaacaagta ttaaagggag cgttggtcat cttatttgct gtaagtacaa gcgtcccgca
     5161 atggacagta tatggagaga gcatttcttc cgaatcggaa tctcgggagg tagaaaccac
     5221 agaaactact gatgctggaa atgaaacgaa atcgagtact tcacaaacag aaacaaatac
     5281 tgaaacaacg gttgagacat ctgttactag cacagatcaa gaaacgaccg ctaccacaga
     5341 taaccaacaa acagaagaag caacagctac aaaaatgtca aacgacgaat cctcttctgc
     5401 taaagtaagt gacaaagaga atgggacaga aaatgatgat atcggtaaag gtgaaaattt
     5461 caatggtaaa gaatatttga attacttcgg taaacagatg acagaaatac ttaatggacc
     5521 aacagaagac atcaagtcaa aattaataga gttattagtc actgagcttg ctgttaatga
     5581 ttatggtgta gataaagaga ttatcaaaca tctgattgct acttatctat tgtacgatat
     5641 cgcttttgca aaaaacagtg tgttgatgtc gatgcttgga ggtacagatg atgggacaac
     5701 gcctacagaa aacacaggct tctatccaaa tacaaaacaa cttcatcaaa ctgtactaaa
     5761 caaacaagat ggcaaaatga tggatgtata tgcatattat gtcgatcaag gttcagataa
     5821 aacagtcatg atccatggcg gatttcgagg gaattggaat aacgggatcg ttacagaaga
     5881 atacaatgat ttttataagg ccgggtataa tttattattt gttgattcca gagcgactgg
     5941 taatagcgga ggcgattatg tgacctatgg acaatatgag tcagatgacg tcctttactg
     6001 gataaatcaa gaagttcgag agcggccatc tcaaaagatt ttactttatg gtggttcgat
     6061 gggagcagct acgatgatgt ctgtattagc gaaagatatt cctgtcaatg tcaaaggaat
     6121 tattgaaaat tgcggatttg catcaattga tgaacaacta cgttttacgt acagccagac
     6181 agtagccccc gcactaccgg atgccataaa aaatcagtta gacatcattg gggatcaaga
     6241 gcatgaagat ttatttatgg gccttttgaa acaatactat tttgatcagg agatgcattt
     6301 ggatacaaaa gctgctctac ctacgattgg aatgtcagat tcattgccta aattgattat
     6361 ccatggaaca gcagatgatg tagtacctgt gtctaacgcg caaaaattat atgagttatc
     6421 aggagggtat aaagatcttt tattagtaga aggagcaggg catggaaagg cacaggaagt
     6481 tgaccatgct gcctacacga aacatgtaac ggactttttg aaagtagtgt tccatgatca
     6541 gataaacgta aagtatgtgg atgaaaacaa taaaagtcta ctcaaagacc aggacgaaat
     6601 ccagctttac ggagcttatg gagaaaatta tatgacagag caaaagacat tcgaaggata
     6661 cgaattagca aatgttgaag gaccgactga agggattttt aatgagacga taccaactat
     6721 tattttctat tacaaaaaaa tcccagtagc tccaccaaaa aagcaagatc cgacagagaa
     6781 tgcagacaat aagggaaaga atgaacctcg agaggatcaa tcaagtgatg atacgcaacc
     6841 gattcagatg acagtgaaag tacaaaaaaa gacagatgca aaaaaaagaa tgttgccgaa
     6901 aactggagaa aagaaacatt cgcttctcat ttattcaggt ggatttttat ctgttagcac
     6961 gattatcaca tggatttggc gaagaaagag agggtaaatg aatgaaatgg ttcaagcgaa
     7021 tagtgattgc agtaccagtt atcatcgtag ttattttagt cactgtattt ttgattaatc
     7081 aaatcacacc aaagccgatt agcttgcttg ttagaaaaca atttgacaca agtgaaaaag
     7141 aacaagtata tgcccgacca gatgatttcc agcaaaaaat cgaacaaatc gatataaaaa
     7201 aagaaatatc ctatccttca aagtacaaaa ataataatat ggatatctac acaccgaaag
     7261 tggaaaagaa aaaattaccg attcttttct ggatgcatgg aggcgcatat gtaggagggg
     7321 ataaaaatga ttgtcgtgat tatttggaat atctatgttc agacacgcag caaatcatcg
     7381 taaatataga ttatgaacga tcaccggaag caaaacatcc tacaccagtt atccaattga
     7441 acgaagccat tcaagcagct aaaaaagaga taaaagatca agcagattgg tcgaatattt
     7501 cgattggtgg aaattcggca ggtgcacaaa ttagtggaga atatttgtta gcacttcaaa
     7561 atgaagaaat taaaaaagct gatcatcttg atccgacttt ggagaacaag cagattacga
     7621 agtttatttc tttgtctggt ctgttggatc cttctgcact tactcaagta agtgataaaa
     7681 tctctagctt tctttatgaa aaatgtggat gggcttactt tgataataag gactttgaac
     7741 aatcagagaa ggtcaaaagt ttagccttgg tcagacatgc ggatcgttgg acgcaaaaca
     7801 cattcttaac ggatggaaat acaaatacgt ttacgaaaca aatggaagaa gtaacttctg
     7861 tatttgagaa agcgggggtg aaagtcacaa aagtagatta tgcggagaaa aatgctgttt
     7921 tgaaccatga atatcagttt gatttcagca ataaacaagc gcaagaaaca tatcaaaagt
     7981 tagtaatgtt tttcaaggag taaagtctta tgatgcaaaa gaggttgtaa tctgtcactt
     8041 agctccaagt cacaaggagc aatttcaata aacaagcttt tcatggttta tgaatattgt
     8101 aacttgtgac tgaggagtta gagatggcaa ggggcacctt ttattcgaaa tttgggaata
     8161 tcacacgatt attgtcacga tctcttttac gtaaatttta taagtcagat ttgttgtttt
     8221 gatcgtttaa aaaaagcaac aaatccgaag cctgcggagg caagaaggga caaaaagccg
     8281 agtggtaagc aaaagaatgc tggttccact aatattccgt tgttattgat ataaggctgg
     8341 gaacaaagat gaatagcaaa taaaatcaat gcactagcga aaaaaagcaa agaaatatgg
     8401 atagctttca taagcagcac ctcctttttt ataattgtat gataaataga ttaagaatct
     8461 ataaatgtga caagtttgtt aggatgtgaa taaaatgatc atctagcgaa cttgaaacaa
     8521 agatggctaa tgataagcaa ttaatgattc agcaatcaga taggtacaaa gtttaccatc
     8581 atacgtctag accaataata gaaaatatgg gaatataata gaaaagattt aggatacccc
     8641 caaaagaatt tccccaaaaa ttcgatgtat cctaaacttt tttattttac ataacaatca
     8701 tatcaactgt gaaataaaat tacaaatgat aacctaaaat gaggccaaaa gccagtgtta
     8761 tcaaggaaat acttaattca ccaaaatttt ttttaatttt ttagaaaata agaaacaaaa
     8821 ataataaaaa aaagaaaaga taaatttttt actactgtat actaactttg ttatcgcatc
     8881 taacaagtaa ctcgaataat accaatacaa ccttctccaa agatgcgatg acatacgttc
     8941 taacccaccg actattgatg ttctgtacca cccttgcaga accaaaaaag ccactaccaa
     9001 atatttggta gtggcttttt ttgttttatg gatttttcgc tttttctgtt atggtgctat
     9061 tctttgattt cagaaatagt atgtcgaact ttagcgatag aacgacgtga ttctttttcg
     9121 ttgtaaaaca tcaaaatcac atatagagag tccaaaatgc ttagttgaga aatacgggaa
     9181 gctaaagctt cagatcggaa ttctgtttct tcagaaatag aaataaaaac gacatcgcca
     9241 agtttagcaa gggaagaatt agcttggcta gtaataacga ttgtttttgc tccagttttt
     9301 cttacggttt cagcaatatg gattgtttct tttgattgcc ctgtgtgaga aatcaaaaca
     9361 gcacagtctt tttccgtcaa taaagaagct tccatcaatt gaatatggta atccgtgctg
     9421 tgagaagttg cgatagggga acgcaaaaat ttatgataag cgtcagttgc tacaatctct
     9481 gaaccgccaa caccgaaaaa atagacacgt ttagattcat tgataatagt agccgctttt
     9541 tttagatcct tttgttgtaa caactttttt gtgtcattta aagttgaaat actagactcg
     9601 aacacctttt gcgccatagt gagctcagta tctgttttct gaatattttc gtggatagaa
     9661 atagcagata gatcattttc ttggatcaac atggccattt taaaatcttt aaatccatta
     9721 taaccaagtt tttttgtaaa ttgaaaaaaa gtagagtcag caatgcccag ttcacttgct
     9781 aaattgctaa taggactatg acttactttg cttggatttt ctaaaatata atcggcgata
     9841 gcctgttctt ttgggctgaa gtcgttgtac attgttttga tttgtagtct tatggagacg
     9901 ctcatgagaa ttcctcattt tcataataag attatcattt tttccgttct cattaatgtt
     9961 aaagatacaa ggagagtagg aaaaaatcaa acaaaaaata tttttgtaaa atctatttac
    10021 aaaaaaattt ttttgtataa tgagggtata agaaatggag gcgctatcat ggaaattggt
    10081 gtaattggtt tgggaaaaat gggattgaat ttagctttaa acttaaaaga gcaccatcac
    10141 aaagtacaag gactcgacat ttcaagtaca gctgttagtc aggcaagaga aaatgacatt
    10201 cctgcttatc aagagatctc tgaatttcta gctacttttg agcagaaaaa aatcatctgg
    10261 ttaatgcttc ctgctggaga gataaccgaa aacatgttaa gaaaactttt tccaaaactc
    10321 ggaccaggag acattattat tgaaggcgga aactcttatt ataaagattc aatccgtcga
    10381 gcagaagagt ttcagaaaaa tgaaatcggt tacttggatt gtgggacttc tggaggaatc
    10441 gaaggagcac gacatcaagc ctgcttaatg attggaggag ataaagaaac ttttctatcc
    10501 gtggaacaat tattcaaaga tgtagcaatt gaaagaggat atctgtatgc tggtgcatcc
    10561 ggaagcggac acttcttgaa aatgatccat aatggaatcg aatatggaat gatgcaagca
    10621 atcggtgaag gattccaact tgtacaaaaa agtgagtatg cttttgactt ggaacaagtc
    10681 gcacgtgttt ggaatcatgg ttcggtgatt cgcggctggc tgatggaaat catggaagat
    10741 cagttaaacg aacatcccaa tttaagtaat tatcggggaa tcgtttctgc atcaggtgaa
    10801 gcaaaatgga cggttgaaac agcgctagaa atggctgttc cagttccagt aattgcaact
    10861 agtttgttca tgaggaatct ttcacaggaa gaagatagtt tttcagcaaa agtagtttca
    10921 gcactaagaa atgggtttgg cggacatgaa atagttgaaa agggtgagta aaatgccaga
    10981 ttatcatata gcaatcgtta attccagcag tttcggaaga gtctttccac accacttgga
    11041 aagattaaag tcagttggag acgttcattt ctttactgtt gatcaagaga ttccaggaaa
    11101 agaactagcg gagttactta aaggattcaa tatcattatt gcaagtgtta cgccattttt
    11161 tcgacgagat ttttttgaaa acaaagatga actgctattg atttcccgac atggtattgg
    11221 ttacaacaat attgatttaa aagcagcaaa agaacaccag acaatcgtat ctatcatacc
    11281 tgctttagtt gaacgaaatg ctgtagcaga aaataatata actaatttgt tgtccatgct
    11341 tcgacgcacg acacaagcac aagcaagagt caaggacgat cgatgggaag atcgggcgga
    11401 gttcgtcggt aggacgctgt ttaacaaaac agttggtgtg attggtgcag gaaataccgg
    11461 aagcggagta atcgaaattt tgcgtaacgg ttttcgctgt gatgttctag cttatgatcc
    11521 ttacaaatca gatttataca tcgaaagctt tggtgcaaaa aaagtagaac ttcaagaatt
    11581 actggaacaa tcagatatca tttgtttatg tgcgaatttg acagaagaga actatcacat
    11641 gatttctgat aaagaagtcg atcagatgaa agaaaatgtt tatctatcta atagtgcaag
    11701 aggcgctcta gttgatgaag aagcgatgat taatggtctg aaaagcggaa agatcgcagg
    11761 atatgccaca gatgttttag aagaagaacc gggacgtcaa gatcatccgt atttagcgtt
    11821 tgaacaagta gtgattacgc cacatatatc ggcctacaca agggaatgtt tagaagcaat
    11881 gggtgaaaaa tgtgtcagag atgtcgaaca gatcagtcag caccttcttc ctgaacgttc
    11941 tgtccagaca aagagcaact atataaaagg atgatgaaga aaatggtgaa cggtttaaaa
    12001 gtgaaaacag gccctcaatt ttatttatat gaagaaggtg gtatttcaaa agtttctgat
    12061 ctcttgaaat catacggcgc gaaaagagta ctggtcaccc acggaacggt atcatgggaa
    12121 aaggcgctac ctaaattagt atttttgaat gacgaaacga ttcaattttt ttatcatcgt
    12181 tattctggag aatgtagtta tgctgaagcc agacgaatag ctacaatcat caaaaaaatg
    12241 aaatcgattt cgtagttggc gttggcggag gaaaactgac cgatctagtg ggttatagtg
    12301 ccaatcttgc aagaatcgat ttcggagtca tccctacttt agctagtaat tgtgctccat
    12361 ggacacctct tgctgtcatg tataaggaga atggacaagc agaagggaaa acagaacact
    12421 tttacagaca agctgctttt tttataacag atccccaatt agtgattgat tcccctgttc
    12481 catatttcat tgccgggtta gcagatacac tagcaaaatg gtatgaatct gagctcatct
    12541 tagaacaaga ttttttgcaa gatgaatcat ttttgaagct agcacaagat actgcgaaga
    12601 tctgcaaaga agagattttg aatcattctg ctaaagcaat ccaagatatg gcacaaagaa
    12661 aactgacatc ggagttcaaa cagctttcag agattatctt tgctgttgct ggtttagttg
    12721 gaggattagg agataaatac gcaagaaatg cggcagcaca tgctatgcat gacgctatca
    12781 gtaaatatat tccggaaagc catcgttttc tacatggaga aaaagtagcg tatgggattt
    12841 tctatcaact ggcactagaa ggtcgctggg aaaaaataga cgagttatat cctttgtatc
    12901 gggaattaga actgccggtt tctctagaac aaatgggatt atttccgaaa gataaacaga
    12961 taatagataa aatggctgaa tttattgatt cgaaagagaa agtgcatttg attcctatag
    13021 aggtaacaca gaaatcactg atacagtcaa tgatggaact ggaaggttat atcagaaaga
    13081 tctgaaggag gaggacacat ggaaatatta agtgtagtac aaagtttgtt gattgcttta
    13141 tgggtagccg cggttatgtc ccgctggtta ggtggaggag ccacgttgac cttacgtttt
    13201 tctcctttga tgactggatt agtcgtcggg ttgatcatgg gggaagttgc acaagcgatg
    13261 attgttacag ccgctttgca aatgatttat atgggggttt tttctccagg aggatcaatg
    13321 ccagctgaac catctattgc agcagctatc gcagtaccag tcgcattatt aggggatctg
    13381 tcgccagaag cagcgattgc cgttgcggtg ccagtcggat tgctcggaag ttacttgtat
    13441 cagtttcgtt ttttcatcaa tactttttta ggtaaatata cagacaaagc tgtagaagat
    13501 ttgaatacaa aagctatggc ccgatcaatt attctttatc caacggttgc ctcattttta
    13561 ttattcgttc cgcttgtttt cattgcttta tatttagggg cgccattgat cgctgatgta
    13621 atcaaagctt tagagggaac agtcgttatc cacattcttg aagtagtagg tggagggtta
    13681 gcggcaatcg gtatcgcgac aactgtatac gttatcggtc gaaaagatta tctggtcttt
    13741 ttcttccttg cttattttat gagtgttgtt ttgaaatctt tagatattac gatggttact
    13801 tatgccattt ttgggattat tatcgctttg atctttgtcc agtcgcaaaa agggaaacaa
    13861 gtacagcctg ccacaagcaa taacaattca gcatcttttg atgacgaaga tgactatgat
    13921 gacggatttt aatttaggag gaatgggaaa tggctgaaat aatggataat caaacaggtg
    13981 ctcctgaaga aatcacgaaa aaagatgtaa caaaagccta tctgagatgg cattttgcaa
    14041 acgaaattcc tcactctttt gaacgttact tggcgccatc actactatac ggaatgatgc
    14101 caatcttgaa aaaactttat aaagatgaag actctttaaa agcagcatat aaacggcagt
    14161 tactgttctt caacactcaa ttaagctggg gtggcggtgt gattactggc ctaatgtctt
    14221 ctatggaaca gcaaagagca gaagaagagt atgaagaaaa agagatcttg atgcaggatg
    14281 acttgatgta taacacgaaa gcgggattga tgggggcact agcaggaatc ggagatgcaa
    14341 tcgattcagg aaccgtccaa tatattttta tcgcaatcgc ggtaccttgg gcacaacagg
    14401 gaagtccttt aggcgcactt tttcctttta tctgctttgg gttgtatcag gttttactag
    14461 gggtattttt cgcaagacaa gctttcacta tgggccgtaa tgctacaggc ctaatgcaaa
    14521 acactggtgt tcagacagcg attgaaatgc tatcagtttt agggttgttt atgatggggg
    14581 tgcttgcggg aaactatgta aaagtagaat cttcattgaa atttgcgata tccggacgtg
    14641 agtttgttat ccaggaaatt cttgatcaaa tcgtgccagg gatattacct ttagctgtcg
    14701 taatgggtgt gtacttgttc tatacgaaaa aaggattgaa agtcacacaa gcattattat
    14761 ggctgactgg aatattaatt gtgctagcag gaatcggtat tttataaaaa gaagggaacg
    14821 tgaataagat gggtgtagtc aatttagcgc gtgtggatga gcgtttgatc catgggcaag
    14881 taatggtaac gttatctcaa aaaagtggag tgaattccat ttttgttgta gacgaagttg
    14941 tagcaaaaga taaatttatg agagatcttt acaaaaatgc aggaagccgt acaggacaaa
    15001 aaacaattgt cattacacca gagaaagcta aattctattg ggatgaatat caatttaaag
    15061 aatacaattg tattttgatc gcaaaaacag tccaaacgat ttacgactta gtaaaacacg
    15121 gaatccccat ggaagaattg aatattggag ggattgctca aaaaaatcct gatacagatc
    15181 ttttagtgac gaaatctgtt tatttaaata aatcagatgc tgaaaaattg aaagaaatga
    15241 atgaacaata tggcgtaaag aatatttatt ttcaagccac tccatctgca cctaaaacag
    15301 gattaaaaga tgtattgaac aagtttgatt tgtcataaac aaatgcttag aaaaaagata
    15361 aagagggcga aaaaatgaag aagaacgagc tgtttcgtga ttgggaattt cgttatcgct
    15421 atatttaccg taagcgccgt acaaaaaaga gtaaacagcg ttttctttca gcattagtat
    15481 cagatattta cagtatgaga acagatgtga cagtcattgc ttacgatacg ctagcctacc
    15541 gatcaaaaaa tatttatgtt ggagatatcg aaaaagcaga gaaagttatc tgtacttatt
    15601 acgatacacc cgttcatgca ttaggaagtt atttcatgtt tgattggaaa gaccaaagga
    15661 aaaaaacaat ctactctata ttattgtcct tcattttgtt gttcagttta ggttggtggg
    15721 gaatgatgat atataacaaa aatcctcatc acgtattcga tctgctatcc gtataaacag
    15781 gtatcactgt actagcattt gggagctact ttttcttatt gggaaaagca gctcgcggat
    15841 ggcctagcag acaaactttt attcgaaata cttcatccat attaacgatg ctagaaatga
    15901 tccgtacgat tgatgatccg agtgtagcgt atgcgtttgt tgatgaaggc tgttatggag
    15961 aaaagggctt agacagcgtg agatcgggta tgaaaaaaga ggctattctt ttttatctag
    16021 actctgttgg ggcagataca ccgctacagt tttcgggaaa ttatttttca aataaagaac
    16081 agtggctgaa acaagttgat aagttgaaag aaaaaaacgt caattatatt tttagtgcaa
    16141 gaaaaaagca agcgcagttc ttctatttga ccaaaacaga tttacgaggg aaaacattta
    16201 attggcaaaa tgccaatcag attattgctc tttttcgata agtaaaaagt gcaaaacaag
    16261 gagtgagaag actaatgttg gaaatcatga tcgttacaca tggtaagttg agcgaaggac
    16321 tgaaagatgc ggcggaagta attgtcggga catcgagcca tattgaaaca atgtgcttag
    16381 agtctgggga ggacatccaa acattcggag agaaggtcaa agaaaaatta actatgatca
    16441 acacagcaga acccatgcta gtatttgttg atctaattag cgcaagtccg tacaatcaag
    16501 cgctcttggc agtcaatgaa ctgactgaag aaaagaaagc agccacttat gtcatagggg
    16561 gcgtgaatct gccaatgttg ttagaagcaa tcaatcatca gatgcttcac acaccgatag
    16621 aagaagcggt ggaacaaatt gtcaaacaag gaaaagataa tttaactgtt tggcatgctt
    16681 cagcggaaga aaaaaaatta gatgaggacg atttttagaa ggaaggctga aaagatgaag
    16741 tggggattta gatggtatgg tgaaaaaggg gattcgatcc cattaaaata tgttcgccaa
    16801 ataccgggaa taaatgggat aattggaact ttgctgacta aaatgcctgg agatgtttgg
    16861 gagatttcag aaattgagga gttgaaagca tctgttgaga gccaaggact ggaactgctt
    16921 ggaattgaaa gtgtagcgat ccacgatgca atcaaggcag ggacagatga acgagattac
    16981 tatattgata actatatacg aacaatcaaa aacttgtctc gatgtggggt tcgattgatt
    17041 tgttatagct tcaaaccaat ttttggatgg gcgaaaactg atttgtttaa tgaaaacaac
    17101 gatggaagct attctctttt atacgatcag aaaatcgtcg atcagatgga gccaggagaa
    17161 atgtatcaat tgatacacag ccagtcaaag ggattcaaat tgccgggttg ggaagaagaa
    17221 cggttgaaaa aattcgctca gctggaaaaa ctgtatgaag gaatgaccga agaagaatta
    17281 tttgaaaatc tgacttattt tttagaaaaa gtgattccag tgtgcgaaaa agcagatgta
    17341 aaaatggcta tccatccaga tgatccgcca tgggaacttt ttggttttcc tagagtgacg
    17401 aaaaatcttg gcgacttgaa gaaaattcta aatgcagtag attctccata taatggacta
    17461 acgctgtgca caggatcttt aggggcaaat ccggaaaacg atttagttga aatcatccat
    17521 gaggtaggtt cgcgcatcca ttttgtccac ttcagaaacg tggagttttt aggagaaaga
    17581 aagttcaaag aaacggcaca tccctctaca gaaggctccc ttgatatgta tcatattatg
    17641 aaagcgttgg tgcaagaagg gttcgacggt cctatacgcc ctgatcatgg tagaaccgtg
    17701 tggggtgaag tggctatgcc tggatatggc ctgtatgatc gtgcaatggg actcacttac
    17761 atgcaaggtc tatatgaggc gataagtaag gaaaaaccac tgggagagca ttatgaataa
    17821 aatcaagctc ttcatctttg atatggatgg ccttttatta gaaactggac gtttagctta
    17881 tcgtgcttat gtaaaatcag cacaaaagta tgattatgaa atgcgtaagg aagtctatta
    17941 tttactaact ggccaaacag aaatggcgat aagagaacaa atgggcattt tgtacggaga
    18001 agatgttcca tatatcaaat ggcgagaagc aatcaatcaa tataaagaaa aaatcgtgaa
    18061 agaagataag agagtatata cgaaaaaagg agcagaagaa attttatctt ttgcaaaaga
    18121 aagaggaatc catacaatcg tggcttcgtc taatacacgg gtaaaagtag aaatgtattt
    18181 aaaaatggaa aatctatatg ccttgtttga tcaaatcatt tcaggggatg atgtaaaaaa
    18241 aggaaagccg gaaccagaga tctttttgaa agcttgttca aaaatgaata ttcctccaag
    18301 tgaagcactt gtatttgaag actcgattgc agggatcgaa gcagcaaggc gagcgggcat
    18361 tttgtctttt ctcatcaaag atcatttgat agggttgccc gatcataatg ggaagcataa
    18421 gttgaaagta gatgtaagtt tatatgaatc caataaagca gactatgaat tttatgacct
    18481 gcatcaagca aaacgatttt tagaaagtaa agggttgtat ctctaataaa gaggactatt
    18541 ttctagtcct ctttatttat tgtcctgcac gactgaggga acagatgaaa aagaagtaat
    18601 tttttatttt gaagtatcta ccttatttgt agtgaacaat ttagaacaaa cacaaaacgc
    18661 atctactgtt atctaccagt agatgcgttt aaagagaaga acgagaatcg tccatatgag
    18721 ggtttccact ccttatgcag atttttttaa ttcttgcact gcttgttcga tcgtaacacc
    18781 tgtcgctaca tgattggtat tttctttgtc aaaaacgaca aatagattca aatctgtatc
    18841 caaggttact cgatattgta tatctttgtt atagaatgtc atagccattc tcctttcttt
    18901 aaatacttgc tgaaaggcaa cgaacgcgtt gataacgaaa ttccatttat aagtatacca
    18961 ccaattgaaa ggttttcata gttaagaaat tttaaagtca aaagttgcag ataaaagcaa
    19021 ataaacaaga aaacaaaaag tgaatcaatc gcggttattc aactttttct tcgattcgaa
    19081 gacaatagaa gacaatgaat tttatatttc attgcattat ccttgtgtta ttagaaaaag
    19141 catgatatat taaatgagta atctattttg aaacgtaatt tgagcgatat atctacatag
    19201 ataaaactcc ttttgcctat caaatattta ttgcaacgaa aaaacgtaca ttgatacgta
    19261 tcaggaggaa tttatttatg aataacggta cagtaaaatg gttcaacgca gacaaaggtt
    19321 ttggctttat cactggtgaa gatggaaatg atgtatttgc acatttttca gctatccaag
    19381 gtgacggctt caaaacttta gaagaaggcc aagcagtgtc ttacgatgtt gaagatggtc
    19441 aacgtggccc tcaagcaaca aacattgtta aagcttaata aaaattgact tttaaccgtc
    19501 ttttcgaaag aaaagacggt ttttttgtac gaaaaaattc attggtcgtt gaatgggctg
    19561 aatagtagtg tataaaacaa ttttgtggag cgtgaagctt ttatggtaga ggaaaatacg
    19621 aataaagtga tgttgataaa aagattccta tcttgaatat gaatcgagat gtcttgaaaa
    19681 tgaatgagtt tgaccaatca ttatcctatc gaaatttaca gtttcgttcg attggtctgt
    19741 caattaatga aaaagtaaaa aagaatatgt tctaggaaga gggcggcaca tctaaatttt
    19801 taagtaacgg acagttcaaa tatcaagcat tgaaagaggc tatcctactg tttgaaggaa
    19861 tacgaatata taaaaacgac aaagaggaaa aagtagttca ttaaatccct tcaagtgatg
    19921 aaagaatgag atctaataaa acctgatctt tcatctgttt ttttaataac gtaataattt
    19981 ttttattttt tgtgctttcg ccgttattgt agagtgattt agcattctta tttttaaaaa
    20041 taactgtgtt atatattaaa aaaggcataa tagttataag cttttttaaa gaatatttag
    20101 aaattattaa ttttcgtctg aaaacaaaaa aagaaagcgt tattatttta gtctattttc
    20161 agggggggaa ccttatgata aaaaaactat ttttactatc aatattaacg ctagcaatgg
    20221 caggttgttc tgactctggc ttagcaactg aactgatcac aaaatcaact gaagccacga
    20281 cagaggagtt aaaatgtgga aacgatcaca gtgactacag aggcttaaat cgacgatgca
    20341 tgggaatatt aggcagaagc aattgtaagc atcgatgtag taatcgaaga aggtgtagaa
    20401 gaagcaacag aaaaatcaaa agaaagatca aaagaagcaa tctgtgctgt gcatcgtata
    20461 cagacgtcga agatgcggta cttttaaacc acgtcaggag atgttcattt atgtctaggg
    20521 aagttggcga tgaaccaaaa ctagagtacg cttttagttt tgcacgtgag gatgaagata
    20581 accctaaaac ttacgacatt ttagcttgat ctaagatggt taaagctttt taatgaagta
    20641 agaggagata gaaggctttt ctagttatat taggtaagat aaattccttt ttgctccaaa
    20701 tttgctaaac attctgctag ataaacttca tcgtgttccg gataaatcgg cacgttgcgc
    20761 tctacagtag gtaaatcaaa aaatgcttcg catgatttgc cacgatacat aggatttttc
    20821 cactctggtg ctgggctgta accacgtttt tccatttcat ccatgacaag ctgatgatat
    20881 tggaataatc tataaggaga atagtgaaaa acataatcca cagtagcatg tttctttccc
    20941 cagccatttc ctcttaatgc acagcattcc cgatgttgtc ctaaaagctg ttgacgagga
    21001 agctttgaaa tcaatttttc atgccataat cgcatagaaa tgtctccttt atatatagat
    21061 tgttaacctt agacgttaaa tgaaaaaacc agcaccaagg ctggaaaaaa tcacttcagt
    21121 ttgcttgctg tttctgagcg accaattagg taatcaacgg aaatatcgta aaagtcagcg
    21181 atatagatta attcaatgat gcttggaatt tcttctcctt cttcgtactt acgatatttg
    21241 aataaaggaa tacttaacgc gctagaaaca gctacttgag ataaagatcg gccagttctt
    21301 aagaaattca gacgacaacg taaagtatcc agtaaaatag cttcttcgat actcatttaa
    21361 tcacctttca ttatagtaga atagttataa tgactataga aagctacaca tgcaatcaat
    21421 agtactcaat gttttttatt taaacgtatc aaataaagaa tgacgatcaa taaagacaaa
    21481 accagtcctg ataattcttc tataaaaaca ctatcgggtt caaagtagtt gtaattaaaa
    21541 actttccaca taaagtagat agccggaaga gtcaaaatag ctaaataaaa ccgttctttg
    21601 ttccaattca taagataaac aatacttgcg accagtgcga ccgcaggcac ccaaaacaaa
    21661 actctcacca acgtaatcat gtaacggact cctcaacgat tcatataata aaagataaca
    21721 tgttattgca tacatcacaa atgaaatgct aggaaaataa aagaagaata gatcaatgaa
    21781 aaatgaaaaa gcagaacagt tttctgctga taagcaggaa ctgttccgct gaatgtgtct
    21841 tatctatcga gaaaaaatca atggattgat atctaagtaa ttcaatacat ataaaaagag
    21901 gacgatcgca ttcactaaaa aggaaagaat gatgatccca tagcgaatgg aaacatattt
    21961 aacaaatact tttgtttgaa tacgggcata tctttttcct ttatagctga gcatcatgac
    22021 aagaagcaaa agggtaatca caccttgaaa aatcattcca ataaacagaa ttcctgaggt
    22081 ttgataattt aattggagaa aataagctgc tgccgcaact aaatcgaata aaatcgcaat
    22141 aaatggcaaa ataatcactc ctaacattga gaataaataa aagaataaca ttttttctaa
    22201 aataagcaaa taaaaattaa agtaacaaaa tagttatgac acaagtaaaa gaaataagaa
    22261 taacaatatt attttactta tattttgaaa cttgtgagag tttgtcattc gtgctatagt
    22321 tgaaaagtaa caaaaataaa ttttatttat gatgtacctt tagctcagtt ggttagagca
    22381 gacggctcat aaccgtccgg tcgtaggttc gagtcctaca aggtacataa gaggttgtga
    22441 aaaagctgcc ctgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
    22501 acttggctgt tcttattact gcagatgcaa gcttttgaat accgtttaag gaagaggttg
    22561 tgaaatcagc gttttgacct gagtattagc ttaacaaacg gaaaaatagt tcctcatatt
    22621 ttgccgtttg ttaagctaat accgagggtc agctgattga acaccattta agaaagaggt
    22681 cgtgagcctg ccgtttcact ctgtgtcata aaaaagccaa tctactttga tagaaaaagc
    22741 acgtaggact aaaacagttc tgcgtgcttt ttttattgtg tgttaaatat cgtaaatgaa
    22801 gatagatatt tttatgattc gacgatagag acgttgaaat ctttcaacag ctctaaagcg
    22861 gattctccgt gttttctact ggagactaac attggatcaa caaaaaagtc tgcttcagga
    22921 aaagcactct gcaggcctat cagattagag agtacacaca agtttgtttc aatcccgact
    22981 acttcgatct ctttttgttc ttctttgctt tcaaataaag tttcctggat ttcagctaaa
    23041 gtttcaggtg gaatcgtaaa gtaatacttt ttaatcactc gttcattttc ttgtggagaa
    23101 agaagaggga tcaattgcaa atcttctcgt tcttcttctt tgtccttcag ttcaactggt
    23161 atatcccgtg tgaagagaat atattcatta ttttggcgtg ctttagctaa tctttgagca
    23221 atacgttctg ccaattgatc tgactctgga atataaaatt cgctgtttgg atcaagaata
    23281 tgattttgca tatcaataat aactaacatt ttctttcctc ctacattcat atcgtcattt
    23341 tattcttttt aatagaagtt ttttaaaata taagcaacac cagcttcttc attcgtcagt
    23401 gttacttggt ttgcaatttc ttttacttgt cgctgtgcat tccccatcgc aatactcaac
    23461 ccgctgtaat tcaacatagc aatgtcgttt tcttgatcgc caaaagccat gatgtcactc
    23521 gattcgattc caaggtaatc cgccagaaaa acaagtgaat gagctttagt gatacctgta
    23581 ggcgtgattt ctaagaaatg cggatcagag aatactgatt ggataccatc tgcttgcaga
    23641 tctctttgta gcttctgtag tttttcttca ctcttatctg ttaatgctaa tttaagatag
    23701 ctaagagaag gatcttccaa catatttaaa atttgatcaa aagaatggtt ctgtaaattg
    23761 aaatgttcat aataagggtc atctttgact agatttgatt catcgaatgt cagctgatca
    23821 tcttgggttt ctcggctagc aatgatggat attttcgctt cttgagcata gaagaaggct
    23881 tttctcagtg tatcgctatc tatttttgaa cgtctgagaa ctttcgccat ttttccatcc
    23941 ttcatttcag caatcagtga accattggaa gaaatcaaat agccaccgat tcctaattcg
    24001 gaaaaaatat ctttagcaga aatgctgttc cttccggtag cgacggttac gattccgcca
    24061 ttttcatcaa atttccgaag tgcttggata ttttcttctg gtatctttcc atttcgatca
    24121 agcaaggtac cgtctaaatc aatcgctgct agtttcattt tatcttccta ctttcttttt
    24181 acatatctat tttcctgtta ccttaagatt aaatgagaat ttaaaaaaat gaaactaact
    24241 tgctttgtca ttatgaattt tttcaaaaaa
//
LOCUS       DAZUMM010000040        23827 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000040 DAZUMM010000000
VERSION     DAZUMM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.040"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACG8E6_001515"
     CDS             complement(53..787)
                     /locus_tag="ACG8E6_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS5061933.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="ACG8E6_001516"
     CDS             1114..1524
                     /locus_tag="ACG8E6_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFS5061934.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="ACG8E6_001517"
     CDS             1517..2218
                     /locus_tag="ACG8E6_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFS5061935.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="ACG8E6_001518"
     CDS             2218..3804
                     /locus_tag="ACG8E6_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5061936.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="ACG8E6_001519"
     CDS             3823..4044
                     /locus_tag="ACG8E6_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFS5061937.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="ACG8E6_001520"
     CDS             4041..4802
                     /locus_tag="ACG8E6_001520"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFS5061938.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="ACG8E6_001521"
     CDS             5182..5691
                     /locus_tag="ACG8E6_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS5061939.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="ACG8E6_001522"
     CDS             complement(5766..6305)
                     /locus_tag="ACG8E6_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFS5061940.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACG8E6_001523"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACG8E6_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFS5061941.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="ACG8E6_001524"
     CDS             7318..8169
                     /locus_tag="ACG8E6_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFS5061942.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="ACG8E6_001525"
     CDS             8197..8832
                     /locus_tag="ACG8E6_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFS5061943.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="ACG8E6_001526"
     CDS             8852..9625
                     /locus_tag="ACG8E6_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5061944.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="ACG8E6_001527"
     CDS             10033..10830
                     /locus_tag="ACG8E6_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFS5061945.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="ACG8E6_001528"
     CDS             10808..11221
                     /locus_tag="ACG8E6_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS5061946.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="ACG8E6_001529"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="ACG8E6_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS5061947.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="ACG8E6_001530"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="ACG8E6_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFS5061948.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="ACG8E6_001531"
     CDS             15998..16558
                     /locus_tag="ACG8E6_001531"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFS5061949.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACG8E6_001532"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACG8E6_001532"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFS5061950.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="ACG8E6_001533"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="ACG8E6_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFS5061951.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="ACG8E6_001534"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="ACG8E6_001534"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFS5061952.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="ACG8E6_001535"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="ACG8E6_001535"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFS5061953.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="ACG8E6_001536"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="ACG8E6_001536"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFS5061954.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="ACG8E6_001537"
     CDS             21905..22483
                     /locus_tag="ACG8E6_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5061955.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="ACG8E6_001538"
     CDS             22535..23017
                     /locus_tag="ACG8E6_001538"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFS5061956.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="ACG8E6_001539"
     CDS             23134..23508
                     /locus_tag="ACG8E6_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061957.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4285 c         5115 g         6646 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcacccggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaataa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagtt
//
LOCUS       DAZUMM010000041        23672 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000041 DAZUMM010000000
VERSION     DAZUMM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.041"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..117)
                     /locus_tag="ACG8E6_001540"
     CDS             complement(<1..117)
                     /locus_tag="ACG8E6_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS5061958.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            296..1957
                     /locus_tag="ACG8E6_001541"
     CDS             296..1957
                     /locus_tag="ACG8E6_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5061959.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2027..2374)
                     /locus_tag="ACG8E6_001542"
     CDS             complement(2027..2374)
                     /locus_tag="ACG8E6_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFS5061960.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2458..3399
                     /gene="opp3b"
                     /locus_tag="ACG8E6_001543"
     CDS             2458..3399
                     /gene="opp3b"
                     /locus_tag="ACG8E6_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS5061961.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3400..4443
                     /locus_tag="ACG8E6_001544"
     CDS             3400..4443
                     /locus_tag="ACG8E6_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5061962.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4449..5519
                     /locus_tag="ACG8E6_001545"
     CDS             4449..5519
                     /locus_tag="ACG8E6_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5061963.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5509..6462
                     /locus_tag="ACG8E6_001546"
     CDS             5509..6462
                     /locus_tag="ACG8E6_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5061964.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6474..7484
                     /locus_tag="ACG8E6_001547"
     CDS             6474..7484
                     /locus_tag="ACG8E6_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS5061965.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7769..8590)
                     /locus_tag="ACG8E6_001548"
     CDS             complement(7769..8590)
                     /locus_tag="ACG8E6_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS5061966.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8687..8965)
                     /locus_tag="ACG8E6_001549"
     CDS             complement(8687..8965)
                     /locus_tag="ACG8E6_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061967.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9233..11242
                     /gene="metG"
                     /locus_tag="ACG8E6_001550"
     CDS             9233..11242
                     /gene="metG"
                     /locus_tag="ACG8E6_001550"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFS5061968.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11721..12308)
                     /locus_tag="ACG8E6_001551"
     CDS             complement(11721..12308)
                     /locus_tag="ACG8E6_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061969.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12483..13805
                     /locus_tag="ACG8E6_001552"
     CDS             12483..13805
                     /locus_tag="ACG8E6_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5061970.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13960..14736
                     /locus_tag="ACG8E6_001553"
     CDS             13960..14736
                     /locus_tag="ACG8E6_001553"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS5061971.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14729..15298
                     /gene="rnmV"
                     /locus_tag="ACG8E6_001554"
     CDS             14729..15298
                     /gene="rnmV"
                     /locus_tag="ACG8E6_001554"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFS5061972.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15291..16175
                     /gene="rsmA"
                     /locus_tag="ACG8E6_001555"
     CDS             15291..16175
                     /gene="rsmA"
                     /locus_tag="ACG8E6_001555"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFS5061973.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16626..17255
                     /locus_tag="ACG8E6_001556"
     CDS             16626..17255
                     /locus_tag="ACG8E6_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061974.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17379..18488)
                     /locus_tag="ACG8E6_001557"
     CDS             complement(17379..18488)
                     /locus_tag="ACG8E6_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5061975.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18697..19119
                     /gene="mgsA"
                     /locus_tag="ACG8E6_001558"
     CDS             18697..19119
                     /gene="mgsA"
                     /locus_tag="ACG8E6_001558"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFS5061976.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19306..19404
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19442..19978
                     /locus_tag="ACG8E6_001559"
     CDS             19442..19978
                     /locus_tag="ACG8E6_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS5061977.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20018..21781)
                     /locus_tag="ACG8E6_001560"
     CDS             complement(20018..21781)
                     /locus_tag="ACG8E6_001560"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5061978.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21784..23499)
                     /locus_tag="ACG8E6_001561"
     CDS             complement(21784..23499)
                     /locus_tag="ACG8E6_001561"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5061979.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7639 a         4321 c         4802 g         6910 t
ORIGIN      
        1 gataagggac tgtgacaaga cttttgtcac agtttctttt tgtttattct tttttatctt
       61 tgtatggcaa taatagacaa tataacgaaa attatttaat ggcttttgga agaccatgga
      121 aaaccttgta caaagctatt ttttctgaaa aattccttga aacctcacga aaaaatcatt
      181 attttatgaa aaagacaaaa aaaagtgttg attttttaat aattattcat taaactctta
      241 aacaacttca gaaaatatta attattctga aaattccaat aaaggggtac gtaaaatgaa
      301 aaagaaattt acatttggtt tggttgcggt atgcggcctt gtacttgctg gatgttatgg
      361 cggcagttca gccacaagca gtagttcagc aaaaagcggt tcagcagatg gaggaggcgt
      421 atttaactta gtcgtgcctc aagaaatgcc tacagctgac ttatcagttg caacagatac
      481 aatcagtttt acagcattga ataacgttta tgaaggaatt taccgtcttg atgaagacag
      541 caaaccacag ccggcaggag ctagcgagtt ggcagaagtc agtgaagacg gattaactta
      601 caaaatcaaa ttacgagaag acgcaaaatg gtcaaatggt gatcctgtta cagcagcgga
      661 ttacgtttat ggctggcaac gaacagtaga cccagctaca gcttctgaat atgcttacct
      721 gtatgcgcca gttgaaaatg cagaagacat tacagctgga aagaaagata aatcagaact
      781 agggatcaaa gcagttggtg actatgaatt agaaatcaaa ttgacgaaac aaacaccata
      841 tttccaatat ctgcttgctt tcccatcatt tttcccacaa aaacaatccg tagtggaaga
      901 aaatggggat gcctatgcat ctgcaagtga taaagctgtt tataatggac cattcacatt
      961 agaagatttt gatggaccag gaacagatac agaatggact tataagaaaa atgatcatta
     1021 ctgggacaaa gatacagtaa aactttctga aatcaaagtc agtgtagtaa aagaatcttc
     1081 tactgcattg aacttattca aagatggcca agcagatgac gtgatcttgt ctggtgagtt
     1141 agcacagcaa aacgcaaacg atccagctta tacttctgtt aaagaagcgc ggacaagcta
     1201 tatcgaattc aatcaacgtg aaaagaattc accattcaac aatgtgaacc taagaaaagc
     1261 tatttcttat tctatcgatc gtgaagcttt agtaaaacaa gtactaggtg acggatcagt
     1321 tgtctctaca ggattgatcc catcagatat ggtaaaaagt ccaacaacga acgaagactt
     1381 tgcaaaagaa gctggaaaat tagtttctta tgacaaagat aaagcaaaag aatattggga
     1441 aaaagcgaaa aaagaattgg gcgttgattc tttagaattt gatttgatgg cttctgatga
     1501 tgattcatcg aaaaaagtaa tcgaatatgt tcaaaactca atccaagaaa acttggatgg
     1561 cgtaacagtc aaaccaacac ctgttccatt ctctgtacgt cttgaccgct caacttcggg
     1621 tgattttgac gcagtactag gcggatgggc tgctgactat gcagacccaa gcagctttac
     1681 tgatttgttt gtcacaggta attcatacaa ccgtggtcgc tggagcaatg cagaatatga
     1741 cgaagcagtt aaagcagctt catcaacaga cgctggcgat gaacaagccc gttgggatga
     1801 tttccaaaaa gcaatcaaga tcatctctga tgatatgggt gtcgttccgg tgtaccaaaa
     1861 agcagaaggt cacctagtga atgaaaaaat caaaggtatc gttcaccatg cggcaggcgc
     1921 ttcatgggat tacaaatgga catacgttga agaataatcg ttaataccgt gagcatgaga
     1981 aaaaacgagg ctgcggcaaa tgtcgcaacc tcgttctcat attcacttat cctaacaaag
     2041 aaaaaagcat gaaaatagca gaaagagcaa ttagtattcc ggcaatgatc agccaaaagc
     2101 tgtacatacc gtagctggca gacgagagcg aggaaaattc tggttttttc tttcggttcc
     2161 ttgttcgtgc aagatggacc gatcgctgaa aatgatcgaa gaaacccgaa gaaaagaccc
     2221 ataaaaaacc gccgataatc agaaacggca atgaccataa gaatagatca ttagataatt
     2281 gaatgagtgt aaaggaatgg ttgatgagat tttttattaa aataaccaga atcagtagaa
     2341 aagctactgc gtaggggata gatttttttt tcatttgatt gctcctttat ttttggttca
     2401 ttatctaata atgtagcgaa acatctgtca gaagtcaaaa gctaggggga aacattagtg
     2461 aatagttata tcaaatatgt attaaaacgt gtctttttca tgatcatcac gctttggtta
     2521 attgcaacaa tcaccttttt cttgatgcaa ttgctgccgg gcacgccata tacgaatcag
     2581 gaacgtttga gtcctgaaac aatcgagatg ctaaataaac aagtcggttt ggataaacca
     2641 gttatcgtac aatatgggat ttatctttct aatttgcttc aaggagattt tggaatttcc
     2701 ttccaattta aaaaccagcc agtcgcacat ttgttagctg gaagaatcgg accatccttg
     2761 cagttagggt tacaggcaat tatctttgga acagtcctag ggacgatttt aggaacgatc
     2821 tctgccatga agcaaaatac atgggcagat acatcttcta cactcgtagc gatattaggt
     2881 cgttccattc caaactttgt atttgctgtt ttattacaat atatctttgc aatcaaatta
     2941 caagttttgc caatcgcaaa atgggatggc tttgtgtaca caatcttacc aacaattgcg
     3001 ttggccatgt cgccgcttgc tgactctgct cggtttatcc gtacggaaat ggtggaagta
     3061 ctgcatagtg attatgtgga attagcaaaa gcaaaaggat tgagccgttg ggaaatcgcc
     3121 tttaaacatg gtcttcgaaa tagtttgatt ccattgatga ctttattagg accgttagct
     3181 gtagcattga tgaccggatc actggtggta gaaaatatct ttgctattcc tggtatcggg
     3241 gaacaattcg ttaagtcaat cacaacaaat gattatccta cgatcatggc tgtaacgatc
     3301 ctttactcat ttatgctgat ttttgttatt ttgatcgtgg atctattgta cggtctagtt
     3361 gatccaagaa ttcgtgtttc agaagggagc cgaggataaa tggaaatgat accaaagcgt
     3421 tatgaaacag ttgcagatat ccctgcggat gaatttcttc cattacaaaa aaataccgaa
     3481 gaagaacgtg aacagattac tgcaccttct ttgaatttta tccaagattc atggcgccgt
     3541 ttaaagaaaa acaaagctgc cgttatttca atggttttac tattggtcat cattttgatc
     3601 tctgtgatca caatctttgt ttctcctcat gatccaactg cccaaaacgt ggcttatatc
     3661 aatttgcctc ctcgtattcc gggaatcaat atcgatggat tgaatggaaa agcaatggtt
     3721 ggtggtgaac ttgttgataa atatgctcaa gcaaacgtcc cttcaaacgt aaatttcttt
     3781 ttaggaacag acggtttggg acgtgatgta ttaagccgct tatttatggg aatgagaatc
     3841 tcgttgctga tcgcatttat tgctgcatta ctagatgtaa cgattggtgt agcttacggg
     3901 ttggtttctg gtttgttagg cggacgtgtc gataacgtga tgcaacgttt tttggaaatc
     3961 ttatcaggaa ttccaaacct cgttgtcatg attttgatgt tggtcgtttt tgaaccagga
     4021 attttttcaa tcgtggctgc tatggccatc acaaactgga taccaatggc acgtatcgtt
     4081 cgtgcacaaa caatgaaatt aaaagatcaa gaatatgttt tagctggatt gacattaggt
     4141 gaatcaagaa tgaaaattgc attcaaacat attttgccga atatctctag tgtcattatt
     4201 attcaaatga tgtttagtat tccatctgct attttcttcg aagccttttt aagctttatc
     4261 ggtttaggtc ttacgcctcc gtcagcttca ttaggaacga tgttgagcga agggtacaag
     4321 acattcctgt atctgcctta tctattatgg attccggcag taactttatc ggttatcatg
     4381 attggcttta acttacttgc tgatggattg cgtgatgcct ttgatcctaa aatgaaagag
     4441 tgaagacgat gacgaaaatt ttagaagtga aagacttaga aatctctttt gatacgtatg
     4501 ctggaaaggt acgagcgatt cgcggtgtca attttcattt gaataaagga gaaacattgg
     4561 cgatcgttgg ggaatctgga agtggaaaat cagtgacgac cagaagcatc atgcgcttgc
     4621 tgtcaagcaa cgccaatata gatgctggtc aaatcctctt taaaggtaac gatatcgttg
     4681 gaaaatcaga aagagagatg caaaaaatcc gtggtaaaga aatagcgatg attttccaag
     4741 atccaatgac ctctttagat ccgacgatgc ccattgggaa gcaagtcgct gagtccctta
     4801 gaaagcataa taaagtttca aaaaaagagg gacaaaaagc tgcattagaa ttgcttaaat
     4861 tagttggaat acccgaagct gagaaacgta tcaacagtta tccgcatcag ttctcaggag
     4921 gtcagcgtca acggatcgtg atcgcgattg cactggtatg ttatccggaa atcctgattg
     4981 ctgatgaacc tacaacagcg cttgatgtga ctattcaagc acagattttg gagctgctga
     5041 aagaaattca gacaaaaatc gatacatcaa ttatctttat cactcatgac cttggtgtag
     5101 tagcgaatgt cgctgatcgt gtcgctgtca tgtatggagg aagaatcgtt gaagttggaa
     5161 catcggaaga aattttctac aacccgcaac atccctatac ttggggactg cttggttcaa
     5221 tgccgacatt agatagtgcg aacgatcgat tgtatgctat tcctggttct ccaccggatt
     5281 tattgaaccc gccaaaagga gacgcattct atccaagaaa tgaattcgcg atgaaaatcg
     5341 atgcagaaca agaaccacca ttttttgaac tatcaaaaac acacaaggca gcaacttggc
     5401 tacttgctcc gcaggcaccc aaagtgacac cgccagaaga aatccaacgt cgctgggcta
     5461 tctttaaaga gaaacaaaaa catacttacg gtcaaggagg aattgctgat gccaaataaa
     5521 ctacttgaag ttaaaggatt gaaacagtat ttcaatgtag gaaaaaagaa tgaagtacat
     5581 gctgtaaatg acatcagctt tcacatttat gaaggagaaa cattcggttt agttggagaa
     5641 tctggaagcg gaaaatccac tactggccgt acgatcattc gcttgaatga acctactgat
     5701 ggtgagatat tattcgacgg tcaagatata acgaagatca aagataaaaa aggtttgacg
     5761 aaattccggc atgatgttca gatgatcttt caagatccat acgcttcgtt gaatcctcgt
     5821 atgaaagtca gagatatcat tgccgaagga attgatgtta atggactagc taaatcatct
     5881 gaagagcgtg caaaaaaagt cgatgatcta ttgagaactg ttggattgaa tccaagccac
     5941 ggtactcgtt atcctcatga attttcaggt gggcaacgcc agcgtattgg tattgcacga
     6001 gcattggcag taaaaccgcg ttttatcatt tgtgatgaac caatttctgc actagatgtg
     6061 tcgatccaag cccaagtggt caatttgttg caagatctcc aaaaagaaca tcaattgacc
     6121 tatctattta ttgcacatga tttgtcgatg gttaaacata ttagtgaccg aatcggtgta
     6181 atgcataacg gcttgttatt agagatggga acaagtgatg aaatctataa tcacggtgtt
     6241 catccttata cagaaagttt gttatctgct attcctctac cagatccaga ccatgagaga
     6301 aaacgcagaa gaatcaaata tcaacctgaa ccagatgacg gacaagtacg tcaacttcgt
     6361 gaaatcgcac cagaacattt tgtctatgca actgagcagg aagtcccata ttacgcaaaa
     6421 aaactaaaac gacaaaagga atctcttctg gttgctaact aggagggaca tagatgctaa
     6481 aagcttttaa attccgtatg tatccgacag aagaacaaaa acagcaattg attcgtactt
     6541 ttggatgtgc tcgatttacc tataaccatt tgctgaaaaa gcggcagaaa agttggcaac
     6601 aaacaggagt agccaatttt tcgctcacac ccgctacatt aaaaaaagaa tacccattct
     6661 taaaagaagt agacagtctt gctttagcaa atgcacagct taatttggat cgggcttttc
     6721 gcaattattt caagggacgt gccagcttcc caaagcttaa aacgaaaaaa agtatgtggc
     6781 aatcttatac gaccaacaac caaacccgta cgatctatct ggaaaatggc tacttaaagt
     6841 taccaaaaca aaaagaacta atcaagataa atcagcatcg gccagttgaa ggatcgattc
     6901 gctccgctac gatttctgct cgatataatg aagaatttta tgtcgcacta ctttgtgatg
     6961 tttcgccagt aaaaaaagaa tctttagcta aatggatcgg gatcgcctat catccgaaga
     7021 cattgatcca aacatctcga cctctagaag taactttacc aaaatttcac cagacagaag
     7081 aaaagctgca acatgcacag cggaaattga atgtgaaagt ccgctccgct caccatagaa
     7141 agatcaggtt agatcaggca agcaattatc agaaacaaaa gagaaaagta atggatcttt
     7201 atctgaaaca aaagaaccag cgagaagatt atcttgaaca attatctggg aaattggtca
     7261 aacaatatga ctacttattt gtagaatctt ttccaaaaga agaggcacat gctgattttt
     7321 ctattcacga ctggcataaa ttaataacaa aattgcgtta taagtcccaa tggtataata
     7381 aaaaatttct tttgatcaat accgacggag cagaagaaag caattccgta agaaagagcc
     7441 aagtagtaga aaaaatgggc agacattcgg ttatcaaggg ataaaaaatg aatgaaactg
     7501 ataggagcgt tagggttagc cttgttaata aaaagtctta atgacttttg tttctaggaa
     7561 tttttctttt gaagaaaagc tcactaatta tgacaaacca ataaaacaat agcagaaaaa
     7621 gttcgtacga gctttttctg ctattgttaa ttttttattc agaaagttta cgaatacatc
     7681 aaaattctga aacaaaaaac gtaactcaca aagagttgtt tttgcgtttt ttcttgaatg
     7741 attaggtctg aatcttttgc tcacaacctt attcatggtg ccccggcttg agctgataga
     7801 tttttgaagg acggccgatt cctacaggac gttcaccgat ctcatcgaaa tattggagca
     7861 tagctttctt aaaattggaa tgatcgatcg ttttataatc gatccctaag aatttagcaa
     7921 atacttttcg agcttccgta atcgtgaaat ctttacctaa aacttgtaga acttgcggct
     7981 cgtgttccat tttattgact acacggttga atgctttcaa gatgatttga ctatggtcaa
     8041 aagccaaagt atctttgcca gaagactctc cagtgactaa atcaagcgag atagccactt
     8101 ctccgctaga taaatggatc ttgttactat gacgttctag tgtgaaccag cgtacttcct
     8161 tggcatcatc tccggcgatt agcggttctt ctccaataaa ggctaagtag ctgactgtga
     8221 cgacccagcc tcgcggatct cgattaggcg tactgaatgt atgcagttgc tcaatatttt
     8281 cttttgaaat aaccacacca gtttcttcct ttgtttctct taagacgctt tctccagttg
     8341 actcatcctt ttggacaaag ccaccaggca atgcccatga atttcgataa ggatgccctt
     8401 ttctttggat caacaaaact ttcagctgat cttcttcttt gttatagcac atcagtacga
     8461 tatcgacagt caaagagggc ttgtcatatt ctggacgttc ctgtttttga taccagttaa
     8521 gaaattcttc ttcacttgct ttatgttcat aatataattt ttcttcttct ttagaagcaa
     8581 aacggttcat ctgaccacct ccattgacag taaaagtact ttttacctta ttgtagcaga
     8641 ctctgaaaaa aaaccaagga tttacttgaa aaatctaggt tatcatttaa atatgaaaaa
     8701 taaatggaag tatgagattg aataaaaaga aaagcagata aaacggcaat actttatacc
     8761 gctcttcacg aatggcaaag aagccacaaa taacgatata tacgtcaatg ataaaacgca
     8821 aaatccaagc tgctgtcata aaactgttca aatgcccatg gaggttaaaa taaccataca
     8881 tacgaatata aaaatagctg aatattaaat gtgctaatga aagaaaagca atactaagtg
     8941 taaaaaaaag atgtttcttc tgcatgattg ttccagtccc ttactaagat agatagtctt
     9001 tattatacca cgtgtgaggg gagaaataaa aagccgtttt ttagtagata tgaaacaaaa
     9061 caaaaacccg catttattca acctattcat ccttttaaag gatagattga acgtagatag
     9121 aagtcgttta tccagtcttt ttcattttga aaaaaagcta acagcatgct ttttttatgg
     9181 tatactagca aggaaacgaa ttcgcatcat gcgataaagg aggaagagcc ccgtggcaga
     9241 aaaggaaact ttttatatca ctacgccaat ctattatcca agcggcaaac ttcatatcgg
     9301 caactcgtat actacgattg catgtgacgc aattgctcgt tataaacggt tgatgggctt
     9361 tgatgtgttt tatctcactg gagtggatga acatggtcaa aaaatcgaaa caaaagcaga
     9421 agaactaggt gtaaaaccgc aagaatatgt agataaaatg gcggctgatg tcaagaaatt
     9481 atggaaaacg cttgatatca gttatgataa attcattcgc acgacagatg attaccataa
     9541 agcagctgtt caaaaaatat ttgatcgttt attagaacag ggagatattt acttaggtga
     9601 atatgaaggc tggtactctg tttctgacga agaattcttt acagaaacac aacttgcaga
     9661 agtgtacaaa gatgacgaag gtaacgtgat cggtggtaaa gcgccaagtg gacacgaagt
     9721 agaattggta aaagaagaat catacttctt ccgcatgagc aaatacgcag accgtctatt
     9781 ggaatactat gaagaacatc ccgaattcat ccagccagaa tctcgtaaaa acgaaatgat
     9841 caacaacttt atcaaaccag gattagagga tcttgcagtt tcacgaacaa cttttacttg
     9901 gggagtacca gtcaagagtg atccgaaaca tgtggtgtat gtatggatcg atgcattatc
     9961 caattatatt actgcactag gatatggctc cgaagatgaa agtctattcg aaaaatactg
    10021 gcctgcagat gttcagatgg tcggtaaaga aatcgttcgc tttcatacga tctattggcc
    10081 aatcatgctg atggcccttg atttgcctct acctaaaaaa gtatttgggc atggctggct
    10141 attgatgaaa gacggaaaaa tgtctaaatc aaaaggaaat gtcgtttatc ctgaaatgct
    10201 tgtggaacgt tatggtttag atgcactccg ttattatttg cttcgtgctg ttccgtttgg
    10261 gtcagatggt gtctttacgc cagaagattt tgtttcccgt gtcaattatg atctggcaaa
    10321 tgatctaggg aacttattga atcgaacgat tgctatgatc aacaaatatt gtgatggact
    10381 cgttcctgac tatgcctcac ttgtcactcc atttgatagt gaactgtcta cgacggctgc
    10441 taacgttgtc ggacgttacc atgatgcaat ggaaaaaatg gaattcaata cagcattggc
    10501 tgaaatctgg gtgctgattt ctcgtgcaaa caaatacatt gatgaaacag aaccatgggt
    10561 attagcaaaa gacgaagaaa agaaagctga attgaatagt gtcatgatcc atttagctga
    10621 atctttgcgt atcgcagcga tattacttca gccaatcatg acagaaacgc cagagaagat
    10681 cttcaatcaa ctaggattgg actctgaaac aatgaatctt gaaggattac actttggcga
    10741 atttccttca ggaacaaaag tagttgcaaa aggtacacca atcttcccac gtttagatat
    10801 ggaagaagaa gttgctttta ttcaagaaaa aatgtctgaa ggtactcaaa caaacgaaga
    10861 tacagtcaaa tgggatccag aagaaacaga acttgtttca acgaaagaaa aacaaatcaa
    10921 atttgatgta tttgaaaaag tagaactaaa agtagcagaa gtcatcaact gtcaaaaagt
    10981 agaaggggca gataagttgc tgcaattccg actagatgct ggagacagcc aagaccgtca
    11041 aatcttgtca ggaatcgctg aattttatcc tgatccaagc gaattgatcg ggaaaaaagt
    11101 cgtcattgta gcaaatctga aaccacgaaa gatgcgtgga caaatcagtc aaggaatgat
    11161 cctttctgca gaagcaccag atggctcttt acaagtaatc gaagcaccaa aatccatgcc
    11221 aaatggctca gaaatcgcat agaaattcaa agggggtatg acactagttg tgtcataccc
    11281 cctttttccg aaatttgaga agcaattttc gtaaattcct tcttattact tgaagccaat
    11341 catataatac agaaacagag aactatccaa aataaatcct agaaaaagaa gtacgagctt
    11401 cgctgatttt tgttaattta gaataatact aataggaatc gtaaaaacag aaaaaaagca
    11461 taatgataaa gggtcgggca gattttaatt gattcatctg gtactctttt ttattgtcct
    11521 tacattcata aagtttgaat gcaatgactt tattacatct atatttatat ctaacagtag
    11581 cgttgatgtg ttaattataa gttctatcac tttatgttca attagtttgt gctcttatat
    11641 gtgtgattga aaatatagat aaaaaaacaa tcatcggctt tgacttcgat gattgttttc
    11701 tttattttgt aaaaatccta tcacttgatt ttatggtaac cttttatttt tgtcttcaaa
    11761 cggcttggta agagagcagt aagcagtgca aatgggatcg aaaagacata ccagaaaatc
    11821 gtacgtccga taccataatg ttgatagagc tttgtcaaca tcacgtgtaa caagatcaac
    11881 cctaaaagca aggagccagc tataaaccaa tgattatccc aatgcatgac ttcactaagt
    11941 gccatcactc caaaaggata gaaataaaaa tagatcccga ctaaggtcat ccgccagttc
    12001 tttgtaaatg ttccgatatt caataaaaag aaaatcgctg aaagcaaaat accgataggc
    12061 ggaaaaagcc caaccaaaaa ggaatacaac atcccccaca agatcatcac aggacctttg
    12121 atcaatgcaa cgattgcgat gaacaaagtc agcaatatta gtttttgtga taacgttgca
    12181 acagtcatta atccaaataa aattaatgtg aaccaatgcc aatgggactg ctgttgttct
    12241 ttacgggaaa aatcgacccg gttggataaa taatctgcta atttcggata agaacgctta
    12301 aaatccataa actgttcctc cttcgtggaa aaggtaggtt tagtatacga aaaataaaaa
    12361 agaaatacat ccgtcttaag agggagtttt tcactgaaag tggagaaaaa agaatggtaa
    12421 attaaaatat tctcagttat actgaaaatt ataccaaatt tcaaagaaga aagaggatgg
    12481 gaatgaaagg caaactaaaa cgagaaatca atctgtttgg cgcattagct accgttatgg
    12541 gaaccgtgat cggtgcaggc gtcttcttca aaactgctgc tgttactgct agtactcagt
    12601 ctacaagttt gacattgcta gcttggcttt tgggtggttt cttaacgatc tgtgctggat
    12661 tgacagtggc tgaattagct acagcaatcc cggaaactgg tggagctgta aaatatattg
    12721 aagctgcgta tggaaaattg cccagctttt tattaggatg ggcgcaaagc ttgatttact
    12781 ttcctgctaa tattgctgca ttatcgatta tctttgcgac acaattgacg aacttattac
    12841 aattatctac cgattatctt ttgttgattg cagtgatcac cgctgtttct gtgacagggt
    12901 tgaatttgtt agggacaaaa gtcggtgctt ctgtacaatc agtgacgttg attgttaaat
    12961 taataccaat tgctgtgatt gtcatatggg gattattgac cccaggacaa ggaactgtcc
    13021 agttatttcc aatcgaagct ggaaaagatg ttacttttgt cgaaggattg agcagtgcgt
    13081 tgttagcaac gttgtttgct tatgacggct ggttaggtgt cggtgcaatg gcaggggaaa
    13141 tgaaacggcc ggaaaaagat ttgcctaaag caattattct tggtttgagt tttgtaacag
    13201 tcgtttatct attgatcaat tttgtttttt tgaaaacttt gccgattgat catcttgccg
    13261 gtaatttgaa tgcagcttct gaagcatcag acgtcatttt tggaggaatc ggtggaaagc
    13321 tggtcactat tgggatcttg atctcggtat acggtgcatt gaacggttat acactaactg
    13381 ggatccgcgt gccttatgcg atggctttag aagacgagct acctttcagt aaacagctga
    13441 caaagctttc aaagaagttt acggttcctt atgttcctgc ggttttccag ttagcagtag
    13501 cttgtattat gatgagcctt ggttctttcg actttttaac agacatgttg atttttgtga
    13561 tgtggctatt cagtttactg atttttatcg gtgtctttat tttaagaaaa aaagcgccgg
    13621 aattgccacg tccatataaa gtaccacttt atccaatcgt tccgattatt gcgattctcg
    13681 gtgctatttt cattttaggt atgacgatga tgacgcaaac gaaattagca ttgatcggta
    13741 taggtgttac cttgataggt atcccagtct attatcaaaa aaagaaaaaa cgctcagaag
    13801 agtaaaatat ttcaacaaca gacagaaaaa gtacgaacta ggtcctctag atcttcgtgc
    13861 tttttctatt tcaataaaac aagttcttct attttgattt ttgctacttc tgcaagtagt
    13921 gcttatgtgc taaagtaaga atgaaaagga gagataaaca tgatatttga ttctcatacc
    13981 catttaaatg ctgaacagtt caatgaagat attccagaaa cgattcagcg agcacaagaa
    14041 atgggggtaa cgaaaatggc ggtcgtgggc ttcgatacgc caacgattga aaagtctttg
    14101 caattaagcc atgactatcc aaatatatac agtattatcg gctggcatcc gacagaggct
    14161 ggcagttata cgaaagaaat cgaaaataaa ttacaagaac aattgacttt accaaaggtt
    14221 gtagcattag gggaaattgg cttggattat cattggatgg aagacccgaa agaagtccaa
    14281 gataaagtat ttcgcagaca gattgctatc gcaaaagaaa tgaacctacc aatcagtatt
    14341 catactagag aagcacttga agacacgtat caaatcctaa aagaagaaga tgttcgagat
    14401 atcggtggaa tcatgcatag tttcagtggt gattatgaat gggcaaaacg atttcttgac
    14461 ttagggatgc atatttcatt tagcggtgtc gtcactttca aaaaagcatt agacgtccaa
    14521 gaggctgcag caaatgtacc aatcgaacga ttacttgtgg aaacagatgc cccttatctg
    14581 gcaccagttc cttttagagg aaagcgaaat gaaccaggct atactcgtta tacagtagaa
    14641 aagattgctg aacttcggaa tcttccgaca gaagaagtgg ctgcccaaac atggacaaat
    14701 gcgcaccgat tatttggtct aagcgaacat ggataagatg aagatccaag agattatagt
    14761 cgttgaagga aaagacgata caagaaggct gcgggaagta gttgatgcag atacgataga
    14821 aacaataggg tctgcaatca atgaagagat cctgatgcaa attgaacatg cccaagagac
    14881 tcgtggagtg atcgttttta ccgatcctga tttttcagga gaaaaaataa gaaaaatcat
    14941 tatggaagct gttcctcagg caaaacatgc ttttttgccg cgcacacaag ctaaaccaaa
    15001 gaaaaaggga agccttggtg tcgagcatgc aagtgatgct gctattttag aagcattgaa
    15061 aaaggtcgtg acgccagaca tttcccaaac ggctgtttct gaaatcacta gacaagacct
    15121 tttagactat ggtttaatag ctggggcaca agcaaaagaa aaacgagaaa aactaggtga
    15181 cgagttgcgc atcggttaca cgaacggcaa acaattggaa aaacgtttga agatgtttcg
    15241 tattacgccg aaagaactcg cccaagcaat gaaaatagtg gaggaacaac atgagtgaat
    15301 atcgtgaaat tgcgacaccc tctagaacaa aagagatttt attgaaacat ggtttttcat
    15361 ttaaaaaaag tttaggacaa aattttctga ctgaaccaaa tattttaagg aaaatcgtag
    15421 aaactgcgca aatagatgaa catacgaacg tgatcgaagt tggaccagga atcggtgcat
    15481 tgacggaaca attagccaag catgcaaaac aagtcgtagc atttgagatc gatgatcgtt
    15541 tgatccctgt attggctgat acgatgcaac cctatgacaa tgtgactatt gtacatcaag
    15601 atattttaaa gaccgatttg tcaacagctg ttagagaaac atttcatgag gagcttccgt
    15661 taaaagtcgt agcgaattta ccttactata ttacgacacc gatcatgatg cattttcttg
    15721 aatcagattt ggttgtggat gaactagtcg tcatgatgca aaaagaagta gctgatcgta
    15781 tctctgctga accaggaacg aaagcttatg gttcattatc gattgctgtt caatattata
    15841 tggaagccag tttagcattc atcgtaccaa aaacagtatt tgtccctcag ccaaatgtag
    15901 attcagcgat tttgaaactg acaagaagag atataccggc agtcgaagtg acagacgaaa
    15961 aagcattttt ccgattgaca aaagcggcat ttcagcaaag aagaaaaacg ctttggaata
    16021 acttgcagca ttcgtatggc aaagatgacc agacaaaagc atggttagca aaaagcttgg
    16081 aaacagctgg aatcgatcca aaacgtcgtg gggagacttt gtcattacaa gaattcgccg
    16141 cattaagcaa tgcgatgtca gaaaatatgc agtaaaaaaa gaggttgtga cagaagttgt
    16201 gaaaaagctg tcctgcatca agtaataaga acagccaaac aaaaatagtt cctcatgttt
    16261 ttcgtttgtt tgggcttatt accgcagatg caagcttttg aacaccgttt aagaaagagg
    16321 ttgtgggcct gccgtttaac tctaagtcac aaggaacaat ttcaaaaaac agcttctcat
    16381 gtttttgagt tagttggtct tattacttga tgcaagcttt tgaccactat tatgccgaac
    16441 aaggagatgt gacacaaatt ttgtcacatc tcctattttt gtttcttgat caaaaaattc
    16501 ttatacttga tactcacact cattttgtat ggatttagaa aagaatatgt gtcttgacag
    16561 ttattagtaa gtgagttata ctaagtcgac atttatgtgt caagacatat taggagagag
    16621 aaaaaatgag aaaaaattca gttcgaaaac taacgatttc tgctttattg attgggatgg
    16681 gggtcattat cccaatggtc atgcctaaaa tcatgattcc accagcttcc tttactttgg
    16741 cgtctcatgt gccattattt attgcgatgt tttttacacc aggagttgca gtggctgttg
    16801 cgctaggtac gacatttggt ttctttttaa caacgccggt tatcatcgca ttgcgagcat
    16861 tgtcacatct tgtgtttgcg ttgattgggg cttggtactt gcaaaagcat ccgagtatcg
    16921 ttttaaaaaa tggacagttt acttttgcta attggcgttt ccaagggttc aattttgtga
    16981 tcggtgtgat tcattcttta gcagaaatgc tagtagtcag catcttttac tttattggta
    17041 atatgcctga aacatattat tctcaaggat atttttatac agtattcatc ttgatgggga
    17101 ttggtgggct tatccacagt ttagttgatt ataatattgc ttattttata gcaggaacac
    17161 tcagcaaata ttttgatatc cccgtattta cggcagcaaa aaaagaaaat cggaaagagt
    17221 cgattcaagg gtacgcgaag aaaacgacat attagacatg atctctcaaa aaaaaaactc
    17281 tcatctagta atggataata aatcctctaa gatgagagtt ttttgtccta aaaagagctg
    17341 caccaacatc ttctggcaca gctctaacag ggggcaattt atggaagttt gatgacttgt
    17401 tctgtttctt tatcaaagaa gtggcctttg ctgatgttga aggcaaggtc gactatttcg
    17461 cctggtttat ggaaatctct tgcatctact tttgagatga actcagtaga accagttttt
    17521 gtgtaaagca tcgtttcagc tccaagtaat tcggaaacga caacttctga tttcactacg
    17581 gcttcaggtg aagcttcaag ggctacttgt tcactgtgga tgtcttctgg acgaataccg
    17641 aagataactt cttttccttc ataacctttt tcaactaata ctttgttttt tccttcagga
    17701 atacgaagct taagcccatg tccatctgtg ataacaccat ttttcaatgt cacattaaag
    17761 aagttcatgg caggagatcc aataaagccg gcaacgaaca tattgacggg tgtatcataa
    17821 acttctttcg gtgtaccgat ttgttggata aatccgtctt tcatgataac gattcggtca
    17881 gccatagtca ttgcttctgt ctgatcgtga gtaacataga tggtcgttgt ttcaagacgc
    17941 tggtgaagtt tggcaatctc agcacgcatg gctacacgta atttagcatc taaatttgat
    18001 aatggctcat ccattaagaa gacttttgca tcccgaacga ttgcccgtcc caatgccaca
    18061 cgttggcgtt gtccaccaga taaagcagct ggttttcgtt tcaagtattc tgtcagacct
    18121 aagatctctg ctgcattgtc cacacgtttt ttgatgtctg ctttatcata tttacgtaat
    18181 ttcaagccaa aagccatatt atcaaaaaca gtcatatgag gatacaatgc gtagttttgg
    18241 aaaaccatgg caatatcacg gtctttaggt gccacgtcat tcatgacttt tccgccgata
    18301 tttagttctc cttcagagat atcttccagt cccgcgatca ttcgcaaagt agttgacttt
    18361 ccacaaccgg aaggaccaac aaaaacgata aattcacggt ctttgattga tagattaaaa
    18421 tcagttacag aataattttc tgcattgtca tattttttat atacgttttt cagagccatt
    18481 tctaccataa tacgttccac actttctttt ttagttttat taactattta aagtataaat
    18541 gaaagcgtta actaacttca atgtcatgtt gaacaagaat tttttctttt ttttgtcaat
    18601 gtgctagttt taagctttaa taaatatcca taagaaataa aaactgatac actggagtaa
    18661 ataagtatgt taacaatatc aaaaggagtg tctcttatga aaatcgcttt gatcgctcat
    18721 gaccgaaaaa aacctatgat ggtgaaatta gcaacagcgt ataaaacaat attggaaaaa
    18781 catgaacttt ttgctaccgg gacgactggg cagaggatca cggaagcaac cggcttgccg
    18841 atccatcggt ttaaatccgg accgttaggc ggggatcagc aaattggagc catgatttcg
    18901 gaggatcagt tagatttggt gatttttctt cgagacccgc tggctgcaca gccccatgaa
    18961 ccagatgtaa cagcacttat ccgtttatct gatgtctatg agattccgtt agctacgaat
    19021 atcggaacag ctgaggtgct actaagagga ctggaagccg gcttcctaga ttttcgaaga
    19081 atcgtccatg aaatagacaa gcatccactt tcattttgat ggataagcgt atggaaaaat
    19141 aacgtttaat gttcgcctat atgtagctat aaaagctaaa caaactgata atatgcgata
    19201 taatacgaac gtattggata atataaacaa taatattagt ttttcgtatt tgcagtaaat
    19261 gtgaaaatat gctatagttt ctgtgggaaa agatcccaaa aataatacag agtagaaaat
    19321 aaagcgttaa gtgctaagag gatgggatgt tgcctcttgg acgaaggacg taatgttcgc
    19381 ggttttattt tcgcattcgc tgcatgatac gatgtagcag ctcttaaagg agatgctaaa
    19441 aataaagaaa caccggttag acgcacgtat gattgctatt atgggcttat tgattgcatt
    19501 gatggtcact ttgtcccgac tggtagcaat cgagacccca tttattaaaa tcagtgtcac
    19561 atttattcca caagtgatca tgggcattct ttttggtcct ttttggtcag ggataggcgc
    19621 agttttagct gatttagtag gaatggccct cttctcgaaa tctgcattct ttattggatt
    19681 tacattgaat gcatttattg aaggagcaat ctacggattc tttttctatc gaaaagagat
    19741 cacttggaaa aatgccattc tggctacgct tagtgtcacg ctgatcatca acttattttt
    19801 gacaccatta tggcttgcgt tgatgtacca tgtgccattg ttcagctggg tcgtttgggc
    19861 gccccgcctg ttaaaaacag taatatggtt accgattcaa tcaattgcca tttattacgt
    19921 aggacgtagt attccttaca aaaaaatttt aagaagttta gcgatccacg ctaaataaaa
    19981 aacaagcagt cttcccatcc cagaagactg cttgttttta ttgtgctgtg aattgtgctt
    20041 gatagagccg tcggtaataa ccatcttttt gaagcagctc atcatgggtg cccatttcga
    20101 caatagctcc ctgatccatc actaatatct ggtccgcatt tttgattgtg gacaggcgat
    20161 gggcaatcac aaaactggtt tttccttcca tcattgctaa gaaggcttgt tggatttttt
    20221 tctctgtcaa tgtatcgact gagctggtag cttcatctaa gatcaacata gggggattac
    20281 tgatcatcgt tcgagcaatc gtcaacagtt ggcgttgtcc atctgatatc tttaaccctt
    20341 gaccgccaat cttagtatct aatttttccg gcaaacgttc tacaaattca tacatataag
    20401 aggcttttag ggcatcattg atttctgcgt cacttgcttc cggattccca aaacgaagat
    20461 tttctcgcaa tgtactgtca aatagccaag tatcttgtaa caccattcca aaacttttac
    20521 gtaatgtatc acgttggata gagcggatgt catggtcatc gactgtaatt gttccttttg
    20581 ttgtgtcata aaaacgcatc agcaaattaa caagtgtgga ttttccggcc cctgtttttc
    20641 ctactatagc aatggtttga cctggataag cagtaaaatt gaaatcttcg atcaaacgct
    20701 gcttggggtc ataagaaaaa gaaacatgat caaaagagac ttttcctttg atatttttgg
    20761 gttccaaggc ataggcatct ggcaaatcag gcttttcttc tggctgatcg atcaaatcaa
    20821 atgctcgttc tagaccagaa aaagcggttt gcagttgtgt cgtgattcca gaaagttcga
    20881 taaatggttt agaaaactga cttgagtaga tcgtaaagct ggaaatcaca ccaacagtga
    20941 ttgacgaacc actcttcaat gccaataggc cgccaactaa accaatcgat aaataagcaa
    21001 ggtgatctac aaaacgagaa gacgggtttg tcaaggaaga agaaaattgt gcccgttgtc
    21061 ctttttcata tagatcagca tttagcagtt caaattgctg ttgactagct tcttcttgct
    21121 gaaaagcttt cacgattttc tggttaccga ttcgttcggt cacaaagccc gagatatccc
    21181 caataatcct ttgctgtcta tcgaaatttg tttgtgaagc acgagcaacg atccaactca
    21241 caaggaaaat aattggtgta gaacaaagaa cgaccaatgt cagaagtgga cttagacgca
    21301 tcataaaaat aaacgaaaga atgatgattg ccactccaga aaataattga ttgaaggccg
    21361 cagaaattgc aatggaaata ttgtccatat catttgtaaa tcgactgacg atatttccgt
    21421 gggagttttg atcataataa cgaagtggca atcgattcag atgagcaaat gcatcttttc
    21481 ttaattttgc aacggataag taagctaacc gattgcttaa cacttggatg agccattgac
    21541 tgactacgtt gattaaaacg ataccaccga atagaataag taaatggaac agtcgattga
    21601 agcggacctg tccttctcca atcatttgat cgatactttg accaatttgt aacgtcatca
    21661 caactgttgc aatcccattg atgattccta gtatcacagc tagccaaatg tcttttgcat
    21721 aggcagtaag gtaaggaatg aatcggcgga gcgtctgcaa ggataatttt tttactttca
    21781 taactattct tcctcctgtg atgcgacgat ttcctgatat tctgctgatt gagcaagtaa
    21841 ttcttcatgg gtaccctgag cagcaatctt accttgatcc atcactaaga tatgatgtgc
    21901 ttcttgaata gagcggacac gttgggagat aataatcact gtactgccaa gataagtctg
    21961 tagcgcctta cgcaaatcca agtctgtttg ataatccaac gcacttaaag agtcatctaa
    22021 aatcagtaat gctggcttgg caatcaaagc tcttgcaatc gtcagtcgct gtttttgtcc
    22081 gccagagaaa tttttcccat tttccatgac ttttgtatct aaaccatttg gaagctgacg
    22141 gacaaattct tctgcctgag cgattttcag agcttcccaa cattcttttt ctgtggcgtc
    22201 tttcttaccc cattgaaggt tttcacgaat agtcccggta aatagtacag aagtttgggg
    22261 aacaagagca atttgctgac ggagatgatc cagctgccaa gaacgaacat ccattccgtc
    22321 cattataact gtaccggaac tgacatcata gaaacgcgga atcaattgtg tcaaagtcgt
    22381 tttcccgctt ccggttggac caatgattcc aaggactgta tttcgcttca gctgaaatgt
    22441 aatattttgg agagaaagac cagaatcagg agtataacgg aagtcaacat ggtcaaaagc
    22501 gataattgct tcagaagaaa aatcgggagt agtactgacc tcagcttcaa gaatcgaatt
    22561 ttctgtgtct agcacctctt tcacccgatt tcctgaagct tcggcacgtg tgaagatcac
    22621 gacgaggttt gaaacaacga tcagtgccaa aagcatttga ttcatataat tgatcaaagc
    22681 aagaacttgt ccttgttgca agctaccaat atttactttg atcccgccaa ctgtcagcaa
    22741 acagatgatc ccgacattca tgatcaaggt tgtagcaggt gataataggg cagagatatt
    22801 ggaaacacgt aaataattgt tcgctagatc atttgtgctt ttatcgaact tctctgtttc
    22861 tttttttgtc tgcgcaaatg cgcggatgac acgaacaccg ctaagattct gactaacgtg
    22921 ttcattgagc tgatctagtt tttcttgcac tttttggtag agaggaacgg tttttttgat
    22981 gataaaaaat aaaatgattg agaaaatagg caataatgct aagaaaaaca gacctacttc
    23041 ccagtcgatc acaaaagcca tgaccactga cccgatactt aaaaacggtg ctctgatcaa
    23101 taaacgaatg accattgcta aagctagctg aagctgattg atgtcgtttg tcattcgggt
    23161 gatcaaggta tctgttccaa aatgattcaa ctcgttgtga gagaaagtat tgattttttt
    23221 gattagctga ttacgtaact ctgtaccaaa tccttgtgaa gctactgaag catagtattg
    23281 gcaaatgata gcacaaatca gaccaatcac agacatagcg accatccata agcccatttc
    23341 aatgattttt cctcggtcat tcatttttag tccattatcg atcaaggaag ccatcattaa
    23401 aggcaggaca agttcgaaga cagcttcaag aaatttgaag aacgggccca aaatgatttg
    23461 cttacgatag tcttttgcat aacgtaataa ctgaatcata tgattcctcc tattatttat
    23521 ttgtccacaa ttgaaaaaac aagtactacc acattgtagc ttaggaaatt tagaatgaaa
    23581 agagaacaag ccttttcata aatgaaagtt ttattatgaa gaagctttag aaattgaata
    23641 gactattttt tttaaaaaaa agaaaatgag aa
//
LOCUS       DAZUMM010000042        23572 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000042 DAZUMM010000000
VERSION     DAZUMM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.042"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACG8E6_001562"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACG8E6_001562"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFS5061980.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="ACG8E6_001563"
     CDS             complement(1173..1868)
                     /locus_tag="ACG8E6_001563"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFS5061981.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="ACG8E6_001564"
     CDS             complement(1881..2597)
                     /locus_tag="ACG8E6_001564"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFS5061982.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="ACG8E6_001565"
     CDS             2927..3532
                     /locus_tag="ACG8E6_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFS5061983.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="ACG8E6_001566"
     CDS             3857..4180
                     /locus_tag="ACG8E6_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFS5061984.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="ACG8E6_001567"
     CDS             4362..5732
                     /locus_tag="ACG8E6_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFS5061985.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="ACG8E6_001568"
     CDS             complement(5862..7163)
                     /locus_tag="ACG8E6_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS5061986.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9384)
                     /locus_tag="ACG8E6_001569"
     CDS             complement(7393..9384)
                     /locus_tag="ACG8E6_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFS5061987.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="ACG8E6_001570"
     CDS             complement(9528..9794)
                     /locus_tag="ACG8E6_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5061988.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACG8E6_001571"
     CDS             complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACG8E6_001571"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFS5061989.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACG8E6_001572"
     CDS             complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACG8E6_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFS5061990.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13138..13701
                     /gene="def"
                     /locus_tag="ACG8E6_001573"
     CDS             13138..13701
                     /gene="def"
                     /locus_tag="ACG8E6_001573"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS5061991.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13954..14475)
                     /locus_tag="ACG8E6_001574"
     CDS             complement(13954..14475)
                     /locus_tag="ACG8E6_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFS5061992.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14478..15320)
                     /locus_tag="ACG8E6_001575"
     CDS             complement(14478..15320)
                     /locus_tag="ACG8E6_001575"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFS5061993.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15313..16038)
                     /locus_tag="ACG8E6_001576"
     CDS             complement(15313..16038)
                     /locus_tag="ACG8E6_001576"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFS5061994.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16214..16510
                     /locus_tag="ACG8E6_001577"
     CDS             16214..16510
                     /locus_tag="ACG8E6_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5061995.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16766..16940
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17078..18670
                     /locus_tag="ACG8E6_001578"
     CDS             17078..18670
                     /locus_tag="ACG8E6_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5061996.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18683..19423
                     /locus_tag="ACG8E6_001579"
     CDS             18683..19423
                     /locus_tag="ACG8E6_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5061997.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19591..20940
                     /locus_tag="ACG8E6_001580"
     CDS             19591..20940
                     /locus_tag="ACG8E6_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5061998.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21068..21658)
                     /locus_tag="ACG8E6_001581"
     CDS             complement(21068..21658)
                     /locus_tag="ACG8E6_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS5061999.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21805..23157
                     /gene="brnQ"
                     /locus_tag="ACG8E6_001582"
     CDS             21805..23157
                     /gene="brnQ"
                     /locus_tag="ACG8E6_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS5062000.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6751 a         4746 c         4490 g         7585 t
ORIGIN      
        1 agggggtgcg acaaaagagc ttcttcaaaa aaataagtcg aaatctcaca caaattcgag
       61 aaataatttt cgtaagtttc ttcttattta ttgcatctga ccacttctgt tacaacctct
      121 cgttaaacgg tgttcaaaag cttgcatcta cgataataag cccaacccaa cgaaaaacgt
      181 gaggagctgt tttatttgga tgctcttatc gcttgatgca aagacagctt ttttacaacc
      241 tctacttcac ggtgctcaat cagctgacct tcggtattag ctcaaaaaac ggtaaaatat
      301 aaacagctat tttccgtttc ttctttttaa tacttaggtc aagatgctgt tttcacaacc
      361 tcttgttata aataaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt catcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc
    10261 tgtcaccttc acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt
    10321 tcgttgaacc atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat
    10381 cgatttcagt tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat
    10441 tgacacgatc gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt
    10501 tgacgaatgc accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt
    10561 tgacttcttt ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag
    10621 ctgaagcaat acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga
    10681 tgattccgtt gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga
    10741 tctggatcat ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg
    10801 ttgaagtcaa ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag
    10861 ttaggatttg ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat
    10921 cttttgtacc ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat
    10981 cagttaggat cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg
    11041 gcgctttgat tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt
    11101 gggaagaaga accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt
    11161 cttcttctga agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt
    11221 cccgacgccc tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt
    11281 agtggtggat gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc
    11341 ctagaggtgc gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg
    11401 atccatgtac acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt
    11461 tacgcccgtc aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa
    11521 gatcttctgt gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa
    11581 atttctcagc ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag
    11641 caagtttttc ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt
    11701 cttttttcaa tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg
    11761 cttgaacgat ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca
    11821 atgcaccaag catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg
    11881 cttgtttcgt tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg
    11941 gattcaagac gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa
    12001 atgggatatc tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg
    12061 agcagtctgt ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt
    12121 ctgcaaacat cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac
    12181 ttggacggcc ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt
    12241 cttcataatt gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag
    12301 ctgcacttaa gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg
    12361 ctagctgacc gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt
    12421 tttctgacat aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa
    12481 aactgccgaa agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt
    12541 gatcacggaa ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc
    12601 actctttttg actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt
    12661 tgaacgtcag ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt
    12721 aggccgcggt atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg
    12781 atttcttcag ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga
    12841 gcgtattcgt tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct
    12901 ttcgattatt cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt
    12961 catgtgccta gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta
    13021 atcattaaga aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa
    13081 actataatgc gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg
    13141 attacaatgg atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa
    13201 gtttctcttc cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta
    13261 aaaaacagcc aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta
    13321 gctgctcctc aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac
    13381 ccagaggcag atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat
    13441 tctgttcaag atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta
    13501 ccaggatatg ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa
    13561 aaacacaaga ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat
    13621 atcaacggtg tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa
    13681 ggcgtattgg tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag
    13741 ctttataaaa aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa
    13801 tcgaaattta gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg
    13861 catcttttct tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa
    13921 tagtttctag gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc
    13981 agctagtttt tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag
    14041 atggatgccg cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat
    14101 aaaggaatcg atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag
    14161 gggatgttcc acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc
    14221 ttcttctgtt tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt
    14281 ttcttctgtt tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg
    14341 tagtaaagca atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt
    14401 gctagcactc actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt
    14461 ctctccttcg atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc
    14521 atcaaatcaa catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta
    14581 acaactttat ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct
    14641 tttttcactt gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac
    14701 tctcgatcga tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct
    14761 tcctctactg tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa
    14821 gatttggtca caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc
    14881 gtgacgataa ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac
    14941 tccagaccgc tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca
    15001 ttcggagcga acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa
    15061 ttgaaatttg cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata
    15121 actggctcca tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat
    15181 cgcagttccg ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg
    15241 tcggcaacca gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt
    15301 tcggttttga attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc
    15361 tccgtcttct tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac
    15421 taagattttg gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa
    15481 actcgttcca acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat
    15541 gactccatct tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca
    15601 tgcttgatgt ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg
    15661 acaatgacat tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc
    15721 gtgcaaaccg tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc
    15781 tatttccaaa tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg
    15841 atagaattta tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc
    15901 catcccgtgg tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac
    15961 ccctgcaccg gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc
    16021 tactgttaat tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa
    16081 atttattgtt gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat
    16141 ttatcacaat gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt
    16201 gaggtgaatc gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc
    16261 ctggttagcg ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa
    16321 tctttgggct tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc
    16381 cgtatatcga acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt
    16441 agccatttat ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc
    16501 ggcagcttaa atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt
    16561 ttattgtatt acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat
    16621 tcacattttg attgtttttt cggtaaaagt aaagatttct atatttatca gtattttttc
    16681 aaaaaccagc agttatagat tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa
    16741 ataactgaat acgagatgcc tcaatcaatg aggtagaggt cgcgagaatc attaaccctg
    16801 tggagtgaag caaacatgga gaagcaggaa tagggaggat cgccgaaatg tgcacttttt
    16861 tgcttggagt gtatgttggg acgcaagtta agagcttgcg gactgtctta gcagtgatgc
    16921 taagttgcgc tatgttttgt gagaggttta tcaccttatg gaattttagc ggccttttta
    16981 tatctataaa tcggtcgctt tttatttttt cagataaagc tttaccgcat ttcatctaga
    17041 ccgctcgtgt atcatataat cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt
    17101 tgccgtacta tttgcttttg tcttctcact attcagtgtg ggatctctgg caaatgcaga
    17161 tgaaaaaaca ccgagcgatg agtttcgcgt aggtatggag gctgggtatg cacccttcaa
    17221 ctggtctcaa caaacagatg agaataatgc cttgccaatt caaggccaaa atagttacgc
    17281 cggcggttat gatgttcaaa tcgctaaaaa agtagcagat ggtttaggga aaaaattagt
    17341 catcgtccaa acgaaatggg atggtctggc tcctgccctt caatcaggca agatcgacgc
    17401 gatcatcgcc ggaatgagcc ctactgctga acgacgaaaa gaaattgatt ttaccgatcc
    17461 ttattacgaa tcacaattag tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa
    17521 aagtttagca gatctttctg gagcaaagat cactgctcag ttaaatacat tccactatac
    17581 tgtgatcgat cagataccag atgtgcagaa acaacaagca atggataatt tctcagcaat
    17641 gagaacagct ttagcttctg gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt
    17701 aacggcaact agtgtgaaca aagacttgga aatgcttgaa ttcagcggtg cagatggctt
    17761 ccagacaaac ccggaagatg ttcagatttc tgttggtatg cgtaaaggcg acccggatat
    17821 ccaaaaagta aaccagattc tttctggcat ctctaacgat gaacgaacca aaattatgga
    17881 ccaagcagtc aaagatcagc ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc
    17941 tgatttcact acgatttgga aacaatacgg caatatgttc ttacgaggtg ctggacttac
    18001 cttgttcatc gcattgatcg ggacagttgt cggtactaca cttggattac tgattggtgt
    18061 cttccgttcg gtacctgaat caggaaatgc actgactcgc ttcttccaaa aaatcggaaa
    18121 tgcacttctt tctatctatg tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat
    18181 ggtcatcttc tacggtctgg cactcgcttt tggcatttca ttgaaccgaa cgattgccgc
    18241 tttgtttatt gtctctctca atactggagc ttacatgaca gagatcgttc gtggggggat
    18301 ttttgcagta gatgaagggc agttcgaagc tgcgcaagcc attggtatga cccatggcca
    18361 aacgatgaga aaagttgtta ttccacaagt tttacggaat atcttgccag caacaggaaa
    18421 tgagtttgtt atcaatatca aagatacagc agttttaagt gtaatcggcg tagctgattt
    18481 gttcttccaa ggaaactctg cttccggtgc gaatttccaa ttcttccaaa catttacgat
    18541 cgtcgggatc atctacttag tcatgacctt tacgatcacc agaatcctgc gggcggtaga
    18601 aaagaaaatc gatggtcctt cagcttatac caagcttgaa gaaatcgaca attcaaattt
    18661 gcaggaataa ggaggaagaa tcatgaatga aaaaattatt gaaatcaaac atctaagaaa
    18721 aagcttcggt gaaaatgaag tattgaaaga tatcagcgta acagtcaata aaggtgaagt
    18781 tgtgacgatc atcggttctt ccggttctgg taaatctact ttcttgcgct gtatcaattt
    18841 actggaaaaa ccaacaggcg gcgagatcat ttataatgga gaaaatgtat tagctcctaa
    18901 atataattta cctaaatatc gaacgaatct aggtatggtt ttccaatcct tcaacctgtt
    18961 caacaatatg aatgtcttgg aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga
    19021 taaagaaact gccaaaaaag tagcaatcga aaatctggaa aaagttggta tggatcgtta
    19081 tatcgaagcc aaaccagcac aattatctgg tggacagaaa cagcgtgtcg caattgctcg
    19141 ggcattatca atgaatccag atgtattgct attcgacgaa cctacttcgg cgttagaccc
    19201 tgaaatggtt ggcgaagtgc ttaatacgat caaagactta gcacatactg gcttgacaat
    19261 gcttatcgtg acgcatgaga tggaatttgc aaaagatgta tctgaccgtg tcatttttat
    19321 ggataaaggt gtgattgcag aagaaggtac accagaagat atctttgtcc atccgaaaga
    19381 agaacgtaca aaagaattcc tttcacgtat tttaaatgct taggatcaat agctgattgg
    19441 gacaaagagc tgcggtgttt accgcagttc ttttttatga attactcata aaaggtaaat
    19501 ttctgaaaat tttggaatct tagttgtgtt tttcagaaaa ctctcttaat atggaagtaa
    19561 ttaaactttt ttagttagga tgatttaaaa atgaatgaag agcaagaact gcagcgtgga
    19621 ctgaaaaata gacatgtcca actgatttct attggcggag cgattggaac ggggttgttt
    19681 ttaggttccg gaaagtctat ccagctagct ggcccttcga tattgctcgc ctatcttatt
    19741 actggatgca tctgtttttt cattatgcgt gcgttaggcg aacttctgtt atcaaacacc
    19801 aataaccatt cttttctgga ttttgttgca gaatatcttg gcaaaaaagc ggctttcatc
    19861 actggctgga cgtattggtt ctgctgggta tcgattgcaa tggccgattt aacagccatc
    19921 ggaatgtatg tacgctactg ggcgcctggt gttcctcagt ggcttcctga actgattgca
    19981 ttaggtcttc ttctttgctt gaatatggtg gctgtctcat tgtttggtga gttagagttt
    20041 tggtttgcat tgatcaaagt tgtagcgatc attgccttta tcctcgtagg agcttatatg
    20101 atcctcaccc actatacgac agatattggc acagccagta tcacgaatct atggtctcat
    20161 ggcggattct ttccaactgg tgcaaaagga tttatcttag ctttccaaat ggtaaccttt
    20221 gcttttgctg ggatagaatt agttggatta gtcgctggag aaacagaaaa tcctgaaaaa
    20281 gtgttgccgg aagctatcaa taatattcct atcaggatca tcttatttta cttaggatct
    20341 ctttttgtca ttatggcaat ctatccatgg aacagcttga atccagataa cagtccattt
    20401 gtagaagtat tttcggaaat tgggatcacg attgctgctt ccttgatcaa tttagtcgta
    20461 ttaagtgcag cagcttctgc ctgcaacagt gcaatatata gtacaggacg tatgcttcgt
    20521 tctttagcgc aagaaggcag tgcccctaaa aaattcaaaa agttaacaac acatcatgtg
    20581 ccagggaatg ctttgacatt ttctactatc gtcattttca tttctgttat cttgaattac
    20641 gtgatgccta gtgaagtatt cacactagta agcagtattg ccactacttg tttcttgttt
    20701 atctggagta tgcttgtcta cacgcacatg aaatatcgta aaagtatcct cggcaaaaaa
    20761 gctcattcat ttaaaatgcc gttgtaccca tttagtaatt atctggtatt tctttacatg
    20821 gcctttgtgt gtgtcgtctt gttcctagga aaagatacga gaatcgcttt attactaaca
    20881 cctgtgtggt tcttgcttct attactaatc tatcatatga aatatgaacg taagaagtaa
    20941 gaaagtagtt gtgacagaag tggtcagctt cgagaaataa gaaggaattt acgaaaattg
    21001 cttcttaaat ttttgtgaaa tttcggctta tttcttggag ataaaagctt ctgtcccagc
    21061 ctcttttcta tttccccgga aacaaccgct gataataggc atctgctttt ttataggaaa
    21121 aaatccaaat accagataca aaaaaacgtc cgaataaccc gatcaacggt acaaaaaaga
    21181 caccggcaat caagcataag ttaatcatca tggagataag acttcgacgg tcctttgaca
    21241 aaactctcac gacatgggaa tctggtttat ttgctttcat gatttgcctc atcaagatac
    21301 caaaagaaaa actccacatc gtgtatacta agacataagc catctcaggc caaaaagctt
    21361 cagggttacg cccgacccaa ctagtaacag aaggacataa agtcagccaa aagagcaaaa
    21421 agatattcga ccagaccact cggtaattaa ttcgttctgc catctgaaat atacgatgat
    21481 ggttcaccca gatcaccgca acaaatataa agctgatggt ataagctaat aatgtatttc
    21541 ggatgaccca taaagaatga aagctagatg tattagggat cttgatctct aatacaataa
    21601 ctctcaaaac aatggctacc actccatcag taaatgcttc taatctcgac tttgtcacga
    21661 tgattcctcc tcagatgatt cagcgctttt attttatcac ttacgttatt aaataaccat
    21721 cgttttatat agggaatttt ctgaaaattg ttgcatattt ttcgaaattt tgttactttt
    21781 aaaaaaaccg taaaggagtt atgtatgaca aaaaaattat catttaaaga ctatcttttt
    21841 ataggctcca tgcttttcgg tctatttttt ggtgctggaa atcttatctt tcccgttcat
    21901 ttgggtcaag aagcaggcgc tgctaccttt tgggcgaatc taggtttctt ggttaccggt
    21961 atcggtcttc ccttcttagg ggtgattgcc attggtgtat cgaaaagctc aggtgtatat
    22021 gaattagcaa aacggataaa tcaaacatat gcgatggttt ttaccatctt gctttactta
    22081 gttatcggac cgttttttgc tttacctcgc cttgcaacca catctttcga gattggttta
    22141 gcaccattta tcgatcctag cagacagacg ctatatctag caattttcag tattgtattt
    22201 tttgtgctgg cttggtggtt ttcaagaaaa ccgacaaaga tattagatta tgttggaaag
    22261 ttcctgaatc ctgcattttt ggttttatta ggtgctttgc ttctccttgc ttttttgaat
    22321 ccactaggtt ctgtaaatca agcgcctatt cagccaaatt atcaagagca tgcttttttc
    22381 acagggttta ctcaaggata taacacatta gatgcgcttg ctgctttagc atttgggatc
    22441 atcattgtca caacaatcca aaacatgggt gtaacaaaac ctgctgaaat cgctaaagac
    22501 acgatcaaat ctggtgcgat cagcattata ttgatgggaa tcatttatac tttgctcgct
    22561 tatttaggtg ctatgagctt aggtagcttt gctctaagtg aaaatggcgg gatcacgctg
    22621 gctcagattg ctgatcatta tttaggtact tatggcagta tcttattagc atttatcgtt
    22681 atattagctt gcttgaagac cggtattggt ttgattacag cttttgcaga aacattcaca
    22741 gatttgtttc cgcaaagaaa ttacgcattc tttgttgcac ttgctagtat ccttccttgt
    22801 ttagcggcaa acgtaggctt gacgaatatc attcaatttt cgttgcctgt tttgatgttc
    22861 atctatccat tagcaatgac actgatttta ctagtgttag ttggtccttt gtttaagcag
    22921 cgtacggctg tttatcgaat gactacttat tttacattga ttgcttcgat ttttgatggg
    22981 ttgaacgctt gcccagaaac tatcaaacaa acacctattg ttcaaaatct tttatatgtt
    23041 gcggaaagct acttgccgtt tttcaaattg gggatggggt ggatcgtacc agcagttatc
    23101 ggttttgtta tcggactgat ttggagtttc gctaagaaag aagaagtagc agattgataa
    23161 aagaggttgt gaaagagacg ttcagccttg agcaataaaa agtaattttg aaaaatggct
    23221 tctcacattt ttgcataaat ttgacttaat accgagaaac tgacttttga acaccgttta
    23281 ttgagaggtc gtgaaaaagc cgcttagttt caagtattaa gaaatatttt gtgaaaatgg
    23341 cttctcacat ttttgcataa atttgactta ataccgagaa actggctttt gaacaccgtt
    23401 tattgagcgt ctataaactg acctgagtat tagagaaaca aaacggcaaa gtagctccat
    23461 atactttgcc gttttgtttt gaactaatac cgaagagaag ctgatcaaac aacatttctt
    23521 ttataatata gattttagac atatatatga tataaaaata gttttcgcaa aa
//
LOCUS       DAZUMM010000043        23394 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000043 DAZUMM010000000
VERSION     DAZUMM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.043"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..552)
                     /locus_tag="ACG8E6_001583"
     CDS             complement(<1..552)
                     /locus_tag="ACG8E6_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS5062001.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(678..2607)
                     /locus_tag="ACG8E6_001584"
                     /pseudo
     CDS             complement(678..2607)
                     /locus_tag="ACG8E6_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(2913..3629)
                     /locus_tag="ACG8E6_001585"
     CDS             complement(2913..3629)
                     /locus_tag="ACG8E6_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS5062002.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(3630..5453)
                     /gene="glpO"
                     /locus_tag="ACG8E6_001586"
     CDS             complement(3630..5453)
                     /gene="glpO"
                     /locus_tag="ACG8E6_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFS5062003.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5467..6963)
                     /gene="glpK"
                     /locus_tag="ACG8E6_001587"
     CDS             complement(5467..6963)
                     /gene="glpK"
                     /locus_tag="ACG8E6_001587"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFS5062004.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7099..8559)
                     /locus_tag="ACG8E6_001588"
     CDS             complement(7099..8559)
                     /locus_tag="ACG8E6_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5062005.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(8549..9844)
                     /locus_tag="ACG8E6_001589"
     CDS             complement(8549..9844)
                     /locus_tag="ACG8E6_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5062006.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10176..10853)
                     /locus_tag="ACG8E6_001590"
     CDS             complement(10176..10853)
                     /locus_tag="ACG8E6_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFS5062007.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(10883..11974)
                     /locus_tag="ACG8E6_001591"
     CDS             complement(10883..11974)
                     /locus_tag="ACG8E6_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFS5062008.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12075..12509)
                     /locus_tag="ACG8E6_001592"
     CDS             complement(12075..12509)
                     /locus_tag="ACG8E6_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS5062009.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13093..14550)
                     /locus_tag="ACG8E6_001593"
     CDS             complement(13093..14550)
                     /locus_tag="ACG8E6_001593"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5062010.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(14547..16442)
                     /locus_tag="ACG8E6_001594"
     CDS             complement(14547..16442)
                     /locus_tag="ACG8E6_001594"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5062011.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(16500..18275)
                     /locus_tag="ACG8E6_001595"
     CDS             complement(16500..18275)
                     /locus_tag="ACG8E6_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS5062012.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18325..19821)
                     /locus_tag="ACG8E6_001596"
     CDS             complement(18325..19821)
                     /locus_tag="ACG8E6_001596"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5062013.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20015..21637)
                     /locus_tag="ACG8E6_001597"
     CDS             complement(20015..21637)
                     /locus_tag="ACG8E6_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS5062014.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(21965..23278)
                     /locus_tag="ACG8E6_001598"
     CDS             complement(21965..23278)
                     /locus_tag="ACG8E6_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS5062015.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
BASE COUNT         6712 a         5131 c         3842 g         7709 t
ORIGIN      
        1 ttttctttga aacatctttc ggtactcgac agcatacaag acaaaagatt gtaaaagacc
       61 gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt ggtaattctg ggaaaagcag
      121 caaaaggatc atgccaacat agatgacagc agtcgctact ttgccatacc acttcgctcc
      181 acttaatttg atatttttag atagcccgat taatccagct acaaacataa agccttcttt
      241 tatcaagaat agaccaaaca acagccaaag ccacggtcga taccatgcca tagacaaaat
      301 cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc accttcccta agtctgttac
      361 ctgattatat ctccgagcaa tgaccccgtc tagataatca gacaaacctg agacaacaat
      421 gaccgctcct gctatataaa atgactgtct cagcacaaaa taaagataaa tcggcagcaa
      481 aaaaatacgg aatatcgata aaagattcgg aatcgtttgt atttctttac gccagtctgt
      541 gcttaatttc attttatcgc cctacctcct cagcactctt ttctttttat tttagcttac
      601 acggggaata aaaccaatga tttcactgtt tttatttttc tttacagaca ctgatattat
      661 gagctaattt gagataatca cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt
      721 ttgcgcattt cttttactag tactttatga gctttttttg atcctcgctc taggtcacgc
      781 tgccgtttac gtgttttata atcgataaac agactgtcta aatcgtaacc tgtcgggtac
      841 agatcttctg ctgaacgcac cagacgaaca cgctgacgag gcacgtggat tatttttttg
      901 tctaaatata catcaatcgt atcttcacct atatcttcat aaactaacgc ttctttttgg
      961 tattcattga caaatatccg atcacctttt gcaaagagct gacgttcttt tttattctcc
     1021 ttcctatttg taactgctgt cttaggaaga aattcttttt tctgtgtccg ataggctttt
     1081 tccgccaagt aacgctctgc ttgatggatc aatttcatcg acatatccat tttatgagca
     1141 atccacaaag cttggctttc tcctgtttct tccaacccgc aactgatatt taggacgaag
     1201 tgcttcccga tcgaaagcca tagcagccgg cacaaaatct tcatgatctc cggcgaattt
     1261 cttgatttct ccataatggg tcgtggcaat gatcaatgcc ccttgctcat acatagattc
     1321 catgattgcg atcgctagtg cagccccttc atttggctct gttccactgc caatctcatc
     1381 taaaagtacc aacgtattcc tttttatctt tttcaaaata gcagcgatgt tttgcatatg
     1441 accagaaaat gtactcaacg cattttctaa attttggtgg tcaccgacat cgacgaaaat
     1501 ctcatcaaag acagcaagct ctgtcccttc ttttgccgga acttgcatac cgaacatcgc
     1561 catcaaagtc aataaaccga ctgtcttcaa cacgactgtt ttgcctccgg catttgctcc
     1621 ggtgataatc aagcctcgat agtctttacc gatttcaaca tccaatggta ccgcatgctc
     1681 aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt tccgatttat tgattctagg
     1741 tgtgcttcca ttgatttctc ggctaaattt tgcccgagca aagatgatat ctaacaccgt
     1801 gatcgtttcg atcaataaat cgatcacttc ttcgttttct gcgatagcac cagtcaatgc
     1861 cgccaggact tgatattctt cactgatttc ttcagctttt aacaactgat agtgttcatt
     1921 caatttagaa atcacagttg gttcaataaa aaccgtcgtt cttttatttg attcatcgat
     1981 aatcgttcca tctattttat ttttatagct ggctttgata ggaatcgtat agtattcgcc
     2041 tttttgaaca atcattgctt cttggatcat ttctttgttt ttcggatgac gcaagaattt
     2101 cagtaatttt gactggattt ctttttcgat tagttgtaat tgtttgcgta ctttacgcaa
     2161 attacgtgac gcgtcgtcac tgactttatt attttttatt ttctggtcaa tcaactcttc
     2221 tacatttatc aaatcaggaa gatgtttgct ataagcaaac aacaagggtg tctggtattg
     2281 attcttatca aaaaactttg tgatcatccg actgcttcta agaaaatccg catattcgat
     2341 caaatcggtt ggctgcagaa tcaacccttt tttgacttgt tctgtcagtg catcgatcct
     2401 cggcaacccc ataaatggaa catgctgact gctttctaag atcaagcgtg cttctcttgt
     2461 ttcttcttga cgatccaaga cagtttgtag gtttgtcgag accgtcatat cgctgattcg
     2521 ttttttgcta tagtttccaa ttgctcttgc ttggatttcc tttttgatcc gatcaaattg
     2581 taattgacta atcatttcgt tgtccatatt ctctcctttt cttgaagaga tacctgtccc
     2641 aaaataaata agggcctaga gataaataaa aggatagaac acagcttaaa aaagtcatgt
     2701 cctatcctta ttagaataca aagatattgt tttcctgaca ggtaccgttt cataagcaca
     2761 ccaaataaac tttgtttatt ggctattcga cttactgatt agataccagc tacactcaca
     2821 aaacataccc cattcttcta ggtcttattt attttccact taactatagc aagacacatt
     2881 ttggttgtca aactttagta tgttttatag atttacacca ttaatgaata gacacctacc
     2941 gcgataattg ctccgatcat tggacctgcg attggtaccc atgaatatgc ccaatctgaa
     3001 gttcctttat tagctattgg caataccgca tgtgctaatc gaggaccaaa gtcccgagca
     3061 gggttgattg catatcctgt tggccctcct aatgaaagac cgatggctaa gatcaatcca
     3121 cctacagcga atacgttcgt tccaggtgca aattcgtttt gtccaaaagc tagcaaccca
     3181 agaactaata caaatgtacc aattaattct gtgatcacat ttgctggata attccgtata
     3241 gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat ctttcgttgc attccaatgt
     3301 ggcaaataag aaagccaaac aagaatggcc cctgcaaaag ctcctaaaac ttgcgcaatg
     3361 atgaaaggaa cgacaagatt ccaactaatt gcccctgtca tcgccatagc tagagagact
     3421 gcaggattca aatgagctgg gcccatgaat ccagaaatat agacagccat tgttacagcg
     3481 agtccccaac caaatgcgat gacaatccag ccagaagcct gtgctttact tttgtttaaa
     3541 ttcacagccg cacacacacc atctccaagc aaaactagga tcagtgtccc aataaactcc
     3601 cccatgattt gagtcatatc actgttcatt tatttctccc cctttagttc ccgcagatct
     3661 gattctgcaa tcaatgcttc taaacgttta gtctgttgcg ccttctcctc ttctgtccat
     3721 tcaaaatatc cagcaattgc atcgatcaca ggttgcttga tctcatcaag ctgatctcgt
     3781 tcaaacaaca gatgatttgt acgacgaatg agataatcgc ctggggccaa aaccatttct
     3841 tcttctaaac cataacgtaa ccgagctgac tcagcgagac tcaaaccagg ataaggtgcc
     3901 atttcttttg ctaattcaaa gatacgtcgg gcattagtcc cgtaaaaatc agcgatataa
     3961 gtagcatctt cttcctctaa accggctgct actccttctt ttgctaattc tgtcactgtt
     4021 tcttctaatt tagttggatc gaagtttcca cctgaaatct gatatttttt agagtcgatt
     4081 tctttcgtct ctattccgta ttcttctttt aataattggc gtatcaatcg caaagctcct
     4141 tctgccatct tacggtaatc ggtgattttc ccaccagata aagtcagcaa accatctggt
     4201 tctctttcta aagaactacc tctggaaatc gtagaaggtg ctttttcatc acggctgttt
     4261 tccaaatggt tcaacacgtc ttctacttca gcacgagaaa ctttgttttc cttatattca
     4321 cttactgtat cgacaacttt attgaagctc ttgtctgaaa tcgatccatt atctccacca
     4381 ttgtaatcag aaccagaatt accaatcaac agtggacgaa gtcctgccca gcttgcttcg
     4441 atatcattca atgtgatatt tgcttctgga tagcgatgat tgatcacatc caatagataa
     4501 tctacatctt cttgtgtgac tttcggatcc gtaaaatctc cttggtaatc cgtatctgtc
     4561 gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa ccatccgccc atcttgcttt
     4621 cctgtatcga agtatgtggg ttgcggtaca ggcagttttt tcgcatctac aactaaatgg
     4681 atccctttag ttggacgcat tttaggagag actggacgcg taaaattcaa gtttcgtact
     4741 ttatctaccc aaggaccact cgtattgatc actaattttg ctttgatttc gatcacttca
     4801 tctgtcagca ggtcacgagc tttgacgcca acgatttgat cgccatcata taaaaagcca
     4861 accgctttca ttttacttac tagataagcc ccatcttcgg cagccttttt gatattatca
     4921 atcactaaac gggcatcatt gttgcggaaa tctagataga cacccgcgcc ttttagccct
     4981 tcttttttca aaaacggttc tctttccaat acttcttctg gtgtcagcgt ataattctca
     5041 tatttggttc ctgtcacatt tgccagtttg tcataaaggt ccattgctac tttgaccgag
     5101 aacatattga aggttgttgc tccttcgccc tcatagattg gcaaaagcat tggatctggt
     5161 tttgggatgt gtggggcaat actttgtacg actgcacgtt caccaactgt gtcagctact
     5221 acttccacat caaatgtctt cagataacga ataccgccat gaacaagttt ggtcgagcga
     5281 gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc ctgttttgat tcctgatgct
     5341 gctgactgga ctgccacacc ggcaccagtg attccgccac cgatgatcaa cagatccaat
     5401 tcttcttgct gcattttttg aatgctatct tgtcttgtct tgtttgaaaa catatggatt
     5461 tcctccttat tcaaatgctt gggtagcttt tactgctttt ttccaaccgt tgtaaagttt
     5521 ttctcggcgc tcttcctcca tttgaacttc aaatagcttg ccagcttcat aaaattcgcg
     5581 gatctcgtct gtatctttcc aataaccgac agctaatcct gctaaaaatg ctgcccctag
     5641 cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca ttcaagatat ctgtttggaa
     5701 ttgcatcaga tattcattgt ttgctgcacc gccatctact ttcaatacag gaatcttgat
     5761 tccagtgtca tcttgcatcg tatcgatgat atctctcact tgataagcaa tcgactgtaa
     5821 agtagcttta atgatatctt cttttgtcgt accacgtgtc aagccaaaca tagcccctcg
     5881 tgctgcctga tcccaataag gagcacctag cccaacgaat gcaggaacaa cataaacctc
     5941 atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg ctagcttttt gcaacatttg
     6001 aagtccatct ctcagccatt gaatcgaaga accagccaca aaaatacttc cttctaaagc
     6061 atagtaaacc tttccattta tgccgtaacc aattgtcgtc aataaattat ttttggataa
     6121 ttgcggtttt tcaccggtat tcatgacaat aaatgaacct gtaccataag tattcttgac
     6181 catgcctggt tcgaatgcca tttgtccgaa taatgcggct tgctggtctc ctgccatccc
     6241 tgaaattggt gcttcgcttc cataaaaatg ataaccttgc gtataaccat aaacttctga
     6301 gttagaagtg acttttggta acatcactcg aggaatattt agtaaatcta aaatctcctg
     6361 atcccaatct agctgatgga tattgaacaa catcgtccga ctggcattcg agtaatcagt
     6421 aacatgtgct tgtccatcgg tcagtttcca tactaaccaa gaatcgatcg ttccaaacat
     6481 caattcgcct ttttcagctc gttcttgcgc gccttctaca tgatcaagaa tccagcgtat
     6541 cttcgttgct gagaaatagg catcgatcac taaccctgtt ttttcgtgga aaaattcttg
     6601 atagccttca ttttttaagt tatctgcaat tccagctgac tgtctggatt gccaaacaac
     6661 tgcatgatag attggacgtc ctgtatcttt ttcccatatt actgttgttt cacgctggtt
     6721 cgttatgcca atcccagcaa tctgatttgg tttgatccct gattcgatga acgctccggc
     6781 aataactgac tgtacagaat tccagatttc atttgcatca tgttccaccc aactttcttg
     6841 cgggaaatat tgcgtgaact ctttttgaga actgccgatg tgtcttcctt tcttgtcaaa
     6901 gataattgct cgtgagctag ttgtaccttg gtcaatcgcc atgatatacg ttgattctgt
     6961 cattgtgatt ccctccatta tatgcaaacg ctttctttaa ctactcttat catactactg
     7021 ttttaaaaaa agaaactgtc aaaattttat tctttttttc tttttttgac agttttttat
     7081 tatgaagcta ttttttaatc aattcttttc ttttttcagc gtctctattc ttcttttcaa
     7141 tcgaataatg tcgtaaggtg acgcaaattc tgacagataa tattcacact gttttttgtt
     7201 tctctggcaa aatgtgatca ccaaatcata ttctttacct tcttcataac ttccaatctc
     7261 aataccgccg attcccctca gttctctgat cagatattgt tgaaaagcaa tacgataaat
     7321 cggcagactt tctaaatcga ttccaatcac tactggtttt gcaatataaa aatcgatcat
     7381 caaaagaaca ttggcatact ggatcatcag gtaactaagc tcactatctt cagctctttt
     7441 cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc agtaactgtg ttaaggaatg
     7501 cccgagcatt ttctgctgct gttccaaaag ccgatcacgc tcatagactt cgatctttcc
     7561 aacaaagaaa aaccattcat tgttgatctg ggaaatttgg tagcctactg ccttttcttc
     7621 tttgattttc aaacgacgag gacgataatg ccataacatc gtttcccgaa tgtaagtatc
     7681 taaaacagcg gttggtaact ttttgctccg tgtcaaatca tactgataaa atatttcttc
     7741 gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc attgtttcgt atacattcag
     7801 ttctgctgct gttcgactaa gatgcatcac gatgattgga tcgattgctt gataaagccg
     7861 gtccgactga tagagtaatt tcttttcttt caaagttgcg tacgtacttt tttcattcaa
     7921 tagtcttttt ttcgtaatcc cgagccagca agcaatctgt gcttctgctg atgccgtaaa
     7981 cgttgtttct aatacacggt ccaatcctag aatgaacggt cttttttctg gtgtattttg
     8041 caaaaaaagc ggtcgctgat cttccgggat atattggaat agttcataat aaaaatatcg
     8101 tatctgtagc tcttctccgt ataattgacc gtttttgatt tgtaatccaa attcctctaa
     8161 gagctggttc aattctttga tctttcgaaa aacagaagac tcactgatat taaacgctgt
     8221 cgctaatcga acgatggata cttgctgatg ttctaataaa tagacgagca tcttaaactt
     8281 caagctttcc tgcaataaaa acaatagaat tttttccaaa cttacatctt cgtccaacac
     8341 aagcagcgcc tgatactcat ttcgctgtac ttctactttt ttttccatca ttcggccaat
     8401 caattcaata tcttccaaat attgatcaaa cgaggctttt gacagttgga catgctctct
     8461 caaaccattg atagaggctg ttcccccttg cagaacaagg tttcgcaaaa tcaggatctg
     8521 taactgctct tttctttcta ataatgcttc aatcttcata ggcagtattc gccacctttg
     8581 accacaccat atctggcaca cggtattctg gctggaaaaa tagctcatgt tcttctaact
     8641 gttcaaacgt ccatccttct tctactgcaa ttgtcagttg gtcaagaagg tggaacatct
     8701 cttttttgct ttggagctgt gctccaagaa tctttttttc agaatcaaat agaaatttga
     8761 gtttaacttt gctttgattt attgctgtaa gcttgtagtc tacgtatcct gtttcaattt
     8821 tttggggata aaaaatgctc tcttcttctg tcaaacctac gcttcctaag aaatagccaa
     8881 accactgatt gccgactgga cgataggttc cctgatcctt tgtctgtgag ccactaattg
     8941 tcttgctgac atttcgtgct gtacggacag cgttatttac tagagaaaca tacatttttt
     9001 catttgttgg acgaaaagtt acttggatcg catcgccaat cgcataaacg tctttttgag
     9061 acgtttgcaa gtagtcatct acccagatcg ttccatcatc attcaatgtt aattgttctt
     9121 cccacatctg attgtcggga cgagaatgat tagccagcaa aacaagatca tagttctcct
     9181 ttcttttttc cgtctctaca atagcggttt cattttcggt caaagacaca acttgttctt
     9241 ctaaaaaaag tgaaagccct tgatttttca tttcctttac taacggttct gtcatttcag
     9301 gatctagata acgaaaaaga attgactttc ggctttcgta aagatggatt tgtttttttc
     9361 ttgatgccaa cccttcagct atttcgattc ctgcttgtcc tgcaccaata attgcaattt
     9421 tctttgcatt catgatcttt tgctgaagag ccgttacgtt ttgtatcttt ttaactgggt
     9481 gaataagcgt tgattctgca gaaatatttc gaaacttttg atttgaacca gtcgctagaa
     9541 tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac gacttttttt tctttcaatc
     9601 ctattactgt cttttctgtg taaacatgga ttcgatagcg gtcagtcagt tcttctttcg
     9661 tgatccaaga aagttgtttt tcactggtaa cctttccttt taatatcaaa ttgaacccgc
     9721 tgggaatcca tcctaacttc gattgctttt caatcaaata tatttcactt gttggcatca
     9781 attggcgaag ataaatagcc gcatgaatcc ctgcaaacga cccgccgaca attacaatgt
     9841 tcatctgcat tcctctttct tataaggtgt tctactgcta acttctcaat cagaaaacac
     9901 agtattcata actcgctttc aaccttatga tacatagcga tgcagcagaa tcaaccctct
     9961 ccatcatacc gtgttctagg tctgcttctc aataatgtac tagtggacct aattgaagag
    10021 tatcatttct gaagagatgt ttcaaaaaat gtactcttat aaaagaaaaa aactcgcctt
    10081 ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa aagacgagtt ttagcgtgtg
    10141 taatgtaata gttagaatag acacatcatt tatttttaat tctttaatgc ttcaacaact
    10201 gcttcgccta attgatgagc agattgagga ttttgccctg taagaagccg cccatcaatt
    10261 tgaaccactt ctgaaaaagc tgctccagac tgatagtttg ctcctttttc tttcaaagca
    10321 tcttctgcta agaatggtac ttcattcgtt gtaccatttg cttcctcttc ttcattactg
    10381 aacccagtca ctgtacgtcc agctaaaata gattttccag actcatctgt aactggcaac
    10441 aatcctacaa caccgtgaca aacagctgag accagccctt catttcgata gatttgtgaa
    10501 gcgactttcc caagatctat tgcttctgga aaatcccaca ttgttccgtg gccaccagca
    10561 taataaaccg ctgcataatc atttgcatcc acatcatcag gacgtaaggt atttcccaag
    10621 gcccgatttc tgaaatcttc atcttcataa taggcccaat cgatttcatc catattcaaa
    10681 acactttgcg gttctaaagg tacatatccg cctttaggac tgacaaaatc tgcatcgatc
    10741 ccatttttta ctaatacatc ataaaaatga gtcagttcac ttaaccacaa acctgtagga
    10801 cgctcgatct tttcgtactt ttctacattt gttaatacga tcaatacttt cattttccca
    10861 tcctctttct ataaaaattg cgttattgat aacgtgcttt caagaaatta tataatgggc
    10921 ttggcaaata aagctcttta tatgcttctt caaaaggttt cgttacaaat tcatcctcac
    10981 ggttttccaa taatttctct gcccagtcag gatcgacgat catttgctgt ccaattgctg
    11041 caagttctgc atatttcaat acctgctcta catcttcgcg attgcggatc ccaccgacac
    11101 caactaaagg gatctttcct tgaatcgtgt tatggaccac ttcaagaatc gatttatcgt
    11161 tgtatttatc agaacgagct attcgatcat acatatttaa tgaaacatgc agataatcta
    11221 gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc ttcaaaacgg atacctggtg
    11281 tttcatattc ttctggtgag aaacgatacc ctacgatgaa tggtttttta gcatattgtt
    11341 caaccgctga gaaaaccgtt gttaataatt cttcaataaa acgataacga ttttccaagc
    11401 taccgcccca ttgatcctct cgtctattcg aatgcggaga gaaaaattgt tggatcaaat
    11461 acgtatttgc tccatgcaac tcgattccat caaatcctga ttcaatcgct ctacgtgtgg
    11521 ctttgccaaa tgcttggatt gtttcaacga tttcttcttc actcattgct cgtggtgttt
    11581 ccgcatcagg acgttttgct gcaactgcac ttgcagatac aggctgttct ccagataaag
    11641 ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat aatcgctttt gcgccttctc
    11701 cttggattgc tttcgctagc tcacttaatc gaggaatcat ttcatcatga gcgatgctga
    11761 gttcacctgg ccagcctttt ccgatatctt ggacatttgc agcaccagta ataacagcac
    11821 caagaccttt agaacgttgt ttataataag tgatctcatc ttcggtgatc actccattga
    11881 aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg gttcttcaat tgaactcctg
    11941 aaggtaaagt aattgattga tcaaattgtg acattgtcat tcctcccata attcgctctg
    12001 attaacctat cgagaatcat ctatcattaa accactcgat tagttgtaca tgcaactaat
    12061 ttgcttaaaa aattttattt tttttgtaac ttatctgttg catcattcat tgtctgggct
    12121 aattctttac ttgcttcatt tcccaaaact gctttgtatg actgatgaag ttcttcccag
    12181 ctaccatcga cttccttagc aaattggcgt ccttgttctg taagataaac taatgacaag
    12241 cgaccttcgc taactgtttt gacgtatcct ttatattcta atttttcaac aaaacgagca
    12301 atcgttgatg gggcggaata gagcatttgt gcaatatctt tttgtggtat tccatctttt
    12361 tcttttaata tcaataaaat aaaaccataa gttggtgcca gacctgtcct tctaaacgct
    12421 tcatcagcta atttattcaa catgcgatct agctttttca cgctaaagaa aagacattca
    12481 tctaacgctt tttcaattgg cttttccatt tcatcccctc cattggcgta catatcatag
    12541 tatgattttt attttttgtc aaaaaacacg gaataatgtc cgcctcctct gtctagattt
    12601 gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt tttcgacaaa actccttctg
    12661 gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt ttgagtatga agaaaaattt
    12721 tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaaa aactggcttt
    12781 tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct gcatcaagta ataagaacag
    12841 ccaaacaaaa atagttcctc atattttgtc gttttttgag ctaatgccga tggtcagctg
    12901 attgtacacc gtttattgag aggtcgtgag cctgccgttg aactccgagt cacaaggagc
    12961 aatttcaaaa aacagcttct catgttttat gactatggtg acttgtgatc gaggagttgg
    13021 caggagaata ctgtttattc gtaaagaagg ggctgtgaca agacttttgt cacagtccct
    13081 tatttccttt tattatagga tttcgccatt tgattggatg acttgtctgt accaatcaaa
    13141 tgattttttc tttaaccgtt tctttgtgcc attcccttcg tcatcttgat caacatagat
    13201 atatccataa cgttttgaca tttgtgaagt agaagcgctg acgatatcaa tgcagcccca
    13261 cgacgtataa ccaaataaat ctactccatc tttgatagct tccttcatct gctcaatatg
    13321 ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt ccatcttctt ctactttgtc
    13381 tatagctccg agtccgtttt cagctatgaa cagtggtttt tgatacctat catataattg
    13441 attcaaagca attctcaaac ccttaggatc aatctgccag ccccattcac ttgatggaag
    13501 atacggattt ttaattcctc cagttattaa gtttccttgg accccttccc aattttctgg
    13561 atgagcactg actgcactag acatgtagta gctaaaagaa acaaaatcaa cagggtaggc
    13621 tgctaagatt tcttcatcac ctacttctat ttttaagttg acaccttttt cttcaaacat
    13681 tcgttgcgta tacgctggat aatatcctct tgcttgaaca tcactaaaga acaaagaagt
    13741 tctttgctgc tcaattgttt tttgaatatc atccggatca caagaatacg gaacaagaag
    13801 ctgatagctg atcatacaac cgacttgtga acctttgata atttcatgac aagcttttgt
    13861 agctaaagca cttgctataa actgatggtg tgcaccttgg tatttcgctt gttccaaatg
    13921 gggatggttt tcttcaatta ctcctacgct aacaaaaggg tgatgtttta cacagttgat
    13981 ctcattgaaa gtcatccaat attttacttt atttttatag cgtttgaata ctgtttcagc
    14041 gaaacgaacg taaaactgaa taagctttcg attaggccat ccaccatatt ctgtaaccaa
    14101 atataacggc atttcataat gggaaagaga aacgactggc tcaatcccat attttagaca
    14161 ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca ttgggtgttt cttcatcacc
    14221 atgaggaaaa attcttgtcc atgcgataga catccgatag catttaaaac ccatttcagc
    14281 aaataaacgg atgtcttctt taaagtgatg atagaaatca tttccgtgtc ttttaggata
    14341 ataatacttg ctgttgggat cctgcgccgc ttttacttgc tgatgcgtca tcatatgcca
    14401 ttgatctgtc catttttctt taggtaaatc gctgtcgaat gtatacacat cagacacaga
    14461 taatcctttt tcatctacat cccatgcacc ttcacattga ttggccgctg tcgctccgcc
    14521 ccataaaaat ccttctggaa attttttcat atcgatcccc ctaaacttgc tagttttctt
    14581 gtttgattat ttttcttatg actagtaatg atatttttga atctactact atttgtaaca
    14641 ataaccattg tggtcggatc atatccctct ttgattaatt cttgcaaatc tacttccatc
    14701 aataaatcgc ctttacgtac tttttttcca tcagatactg cactttgaaa gtatttgcca
    14761 tttaaattca ctgtatcaat accgatatgg atcagtatct ctgctccttc ttcactgact
    14821 accccaattg catgtttcgt tggaaaaact gttgaaactt ccccgtttac agaactgtag
    14881 atttttccgt ccgttggaat gatcgcaaac ccttctccta aagatccact agcaaatgtt
    14941 ggatcgttta cttcagataa atcaattatt tcacctgctg caggagtact taacactata
    15001 tcctttgagg attcttcttc gatttcatcc ttgtagccga aaagaaaagt tagcatacag
    15061 gaactgatca tcgctaagaa ataactagca actagccaaa caaatccttc accaatataa
    15121 atcgggaaag acaaaatact tcttgtagca actgccaaag caccggctcc tgcggcacca
    15181 gctactccac caaatatagc tccacatata caagcaatgt aaaatggtcg tttatattta
    15241 agagtcacac cataaacagc aggttcagtg attccaaata atgctgtgat aaatgcagat
    15301 aaagataaac tttttacttt cttatttttc gttttcaaaa ataccccaag tactgctcct
    15361 gcttgcgcag ctacggcagt catacatgct ggacctagcg tatcacgacc gtagaaagaa
    15421 agattgttgg tcattactgg tacaagtccc caatgtaacc caaaaatcac taacacttgc
    15481 caaaatccgc cgataacagc accagataaa acagggctaa agttataaac agctaaatat
    15541 ccagctgcaa tcagctgact tgcataatct gcaattggac ctacaactaa taaaataatt
    15601 gggaccatca cgatgataca aatgaatggt gtcaataaat ttcgaatact ttcgtgcaag
    15661 cttttgcgaa tcttaggctc aatataagaa agaacccata cagctaaaat tgctggaata
    15721 accgtacttg tataccttgc tagaatcacg ggcatcccta gaaatcgcaa ggttatgcta
    15781 tcactatatg cactgacaat cgtaggataa acgagagctg ctgcaataac catagcgatg
    15841 aaacgatctg tattgaactt ttttgcagca gtatatgcca aaaatatagg caagaaatag
    15901 aaaaatgcat cagctgctgc attcaaaatg atataagttc ctgattcagt agttaataaa
    15961 ttcaaactcg tacataaggc gagtactcct ttgatcaaac cacttcctgc caagacgcct
    16021 aaagtcgggg caaaaacagc ggcgatcatg tctataaaag agtttaaaat gccttttttc
    16081 tcagttcctc tctttccact agatgcatca tcttctagtt tcatttgttt ggataatacg
    16141 tcatacactt catttactgc atttcctact acgacttgaa acattcctcc agcttcgact
    16201 acagtgatca caccttctag ttgctctacc ttctccttat ctgccttctt attatctttt
    16261 agggtaaagc gtaaccttgt tgcacaatgg taaacattgc tgacattttc ttcgcctccg
    16321 acaaatcgga caatatcttt cgctaattgt gaatagtcca tttttacttc ccccttgtta
    16381 attaatgccg tagcaatcct tcttacaaga taagtatagt tcttagaaaa cggtttctcc
    16441 attcaattag taccccttct aatagcgaac gaaactgtac aatagtgtgt atggtttact
    16501 tagccgagca aatcactcac tgcttgaaag aaaacaagta tatcgtttct cttatagtca
    16561 aaggagacaa aaaaaacacg cttgatcttc ctttgctgat gtataagtgg tatctcaagc
    16621 tgatactctg tgatttttgt ttctacttgg cttttgataa gacagcacaa caaagtctcg
    16681 gcatttaacg gaagcataac taggtcagaa ctaggagaga ttagttgctg aatggaatct
    16741 atctttttat ggatttttat ttgattttct tctttgtagt aatgatcaag cagttttgtt
    16801 ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa tctgataaat ctcccaagat
    16861 tcaaactcat ctagaacact aggtagataa atcaaatcat cccactcaaa aataagctct
    16921 tgctcagtag tcaaaattgc atcatattcc gttattttgt ttgattgatt tttgaaaaga
    16981 tacactggtt ctatccggca ttctttgatc cacatgttca tactttgttg aatctgtttt
    17041 tttagtgtat tcaaaatact aattggctgg ttactaacaa tcagtatctc cccttgtttt
    17101 cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat aagcgctttc agctaaattg
    17161 ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta aataaatttc taaaggataa
    17221 cgggagatga tcttttgtac aaaatgattc atggtattat gtccggcatc catcctcgtt
    17281 ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat ggaacaataa cgaaacagga
    17341 agatctaaaa aacgaataat cgtttgctca aatgctctta gattcttctc tatttctttg
    17401 tttttttcat cattaatcat caaataattt aactctaata gtcgattttc aattgcttgt
    17461 ttttcttgat tggaaaaaga atagggtagt tctgatgata acttttttgt gagggtctct
    17521 accatcggaa taaataatgt atacctgttt gttttttcta aaagttcttc tcttcgaaga
    17581 atggtcaaaa cgagataccg agaaaatcgt atgaagtcct ctcctgaaac aatataatta
    17641 tatgatataa gaacctcttt taatgtcttt tggacagtcg gcatgatatt ggaaaaaatt
    17701 gtactgattt gttgaggcaa ccttgcgtgt tggatcaccg tttctgttcc aacaagagat
    17761 aagaaaattc tttttgaatt ttcttcagca atgattttta gtccttttgt cgacgaaact
    17821 tccagttcta atttaggatt gcgctcgatc caacgcaaaa tcgtttttat ttgtaatgat
    17881 acggctgttt ttgaaagaaa gaaaatgtct gctatctttt gcatactgac ataccctttt
    17941 tgaaacaata agtacgtcac aatagttgaa gctcggaaat ccatgctgga atagatttta
    18001 tgtttcgtca cctcttgtat gatttgctgt ttagtttcag attcaattga ttctaaaaaa
    18061 taacctgaac gtttatttga ctggataatg attttcttct cgtagatagc aagcatattt
    18121 ttgatttcag tttggatcgt tttcttgttg gtacctaaag cttcagctaa ttcttcgctt
    18181 gttatccaat gattcttatt tcttaatact acaagaattt ggagttgtcg tttatttaag
    18241 tccatttgct tacacctcct ctttgttata aacatacagg aaaaacctct caaatgacaa
    18301 tagtcacttg cagaggttta aaaatcattt gaagaaagat ctatattcaa ggtttttacc
    18361 attagactgg ataatttgtt tataatattc aaaactatct tttttcaatc tcctaaagct
    18421 tccgctccca tcattttgtc tatccacata aataaagcca tatcgcttcc tcatttcccc
    18481 tgttcctgcc gatacgatat cgattggtcc ccaccataag taaccaatga tttggcaacc
    18541 atcttttatt gcctcagcaa tttcctgaag atgtattttt agatactcac aacgttttgg
    18601 atcgtggatt cgattcaatt catctggtgc ttcatctagt ccaatgccat tttcaactat
    18661 gaataatggt aattcataac ggtctgtcaa ttcattcatg acataacgaa accctagagg
    18721 atcaattggc caactccaag gttcgggaga tttttccttc aaaaagggat tatcgaaacc
    18781 atttgctccg ccagtatcta cgcgcatttc tgcttctttt ttatagacag cgctacgata
    18841 ataactaaat gcaatataat ctgccgtgtt agcttgcaag cactgttcgt ctccatctaa
    18901 cattactggt gcacagtctt cttccttcca tgtcctgtaa atataagctg gataatgccc
    18961 tcggcacatg acatcggaga aaaaccatga attgcgttta aattgcagtg aaccaaatac
    19021 attctctgga tcacagtcat atgcataagt cgctaatgca gaaaagctac acataatacc
    19081 catcttagca tttggatcga ttttctttaa taattgagta gcacgggcgt tcgctagaaa
    19141 catatgatga caggcttgat aaatcacttt ttttgttagt gtagcaatgg gttcaagttt
    19201 atctctagca gggaatgtaa ccaatccagc agctgcataa ggcatacgaa aagcattatt
    19261 gatctcatta aaggtcatcc aatatttcac tttcccttgg tagcgttcaa agacggtctg
    19321 cgcataaaat tcaaaaaagt cgattaattt tcgattttgc caccccccat actctcctac
    19381 caatgctaag ggggtttcat aatgactaag agtaattact ggctcgatat catatcgatt
    19441 taatgtattg attagtctgt cataaaatac gagaccagcc tcatttggct tctgttcgtc
    19501 tcctcttgga aatattcgtg cccatgagat gcttgttcga aaagctttta aacctaactc
    19561 cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa tcaatcgctt catgagaaag
    19621 ataatggagt gattcgtcta aagcaatctc ccaaatcttt ttttcttcat tccactgaat
    19681 actaggatgc tggttcatga tcccgataag cgtatctgtc acattaggta atttgccgtc
    19741 ttccaaccat gcaccttcac attgattagc agctactgct ccgccccata aaaaattttc
    19801 tggaaatatt tcctgatcca taactatctc tcctcgctat caacaatttc ttacttgaag
    19861 tatagctcga ttcatcaaaa caagatattc taactatttc ggcagaagag aagaaaaaaa
    19921 ttgaatgcta ctcttcattt ttaatattta tatataacaa atacataaga cgatccccga
    19981 aagaaaacac tcgttaacca tctaagcaaa cgatttattt tacttccaac acaaatgctt
    20041 cataaggtcg taaatcaact ttgccgtctg gtaactgttt cagaggataa tttgacacaa
    20101 taacccttaa agcttcacgt ggaatagtga acgtctgctt ttcactggac atattacaaa
    20161 taacgagcca ttgctggtct tcatatatgc gagaataggc aaagacatgc tcaggcgtct
    20221 tttccactaa ttcatagtct ccccagacga tgatgtcatg ttttctacgc aaatcgatca
    20281 atttcttgta catataaaaa acagaatctt gatctgctaa cgcttcttct gcattgattt
    20341 ctttataatt tggattgact cgaagccatg gaattccagt agtaaaccca gcatgttcac
    20401 gggcgttcca ttgcataggc gtcctagcat tgtctcttcc tttcgccttg attgattcca
    20461 agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt atacatattg attgtctcaa
    20521 tatcttttgc ttccgcgata tcatcgattg ggcaattcgt catgcctaac tcttctcctt
    20581 gatagatata aggggtgcct ttcatcatat ggagcaagat agcaaacatc ttggcacttt
    20641 cttttcgata cacatcgtca tttccccaac gagaaacaat tcgcggaaga tcgtgattat
    20701 tccaaaataa actattccat ccttcatcac ctaaagatgt ttgccatttg gataatactt
    20761 ttttcagttc agagatactc aagggtttca aatcccattt gtctttgcct tcttgctgat
    20821 ctaatcctat gtgttcgaat tggaagacca tggataattc atttctttta ggattggaat
    20881 aaagtttagc gatttcggta gttgctcccc atgtttcgcc aacagtcatc agatttttgt
    20941 caccaaaagt tgcttgattc atttcctgaa gatactcatg cagtttaggt ccattacttg
    21001 tgatttcttt atccggttct ttgccaatca tatcgatcac gtccatacga aagcccccaa
    21061 tacctttatc gatccagaaa ttcatcattt gataaatctc ttggcgaacc tgtttatttt
    21121 tccaattcaa atcaggttga cgcttgctat acaagtgcag aaaatattgc cccgtttttt
    21181 catccaactg ccaagcagaa ccactgaata ttgagcgcaa tctattaggc tcttttccat
    21241 tgaccggatc acgccaaata taatagtccc gataaggatt ttctttattt ttacgggcct
    21301 caatgaacca cggatgttca tcagaagtat gattcaccac cagatccatg atgatacgga
    21361 tatttctttt ctttgcttct tcaatcagtt cttccatttc ttccatcgta ccgaattctg
    21421 ccatgattgc ttgataatta ctgatatcat acccattgtc atcattcgga gactcgtata
    21481 ctggagaaag ccaaattgca tcaataccta aatacgccag ataatccaac ctggaaataa
    21541 tcccccgcaa atctccaata ccatcttgat ttgaatcttg aaaacttctt ggatagattt
    21601 gataaacaac tgaatggtgc caccattttt gttccatgat tttcctccta taaatggatg
    21661 tttctatttt ttgcttaatc atttatttta gtctctctca ctaaaaattg taacgctttc
    21721 aaatagctat tgcaccttgt tatcggtaac agaataaaac aaaaaaacag ctgaggaaat
    21781 tccctcaact gttttttgaa aggattgatt tatgcaggga agaatgctga tttcacaact
    21841 tctttataaa cggtgttcac tcagctgtcc cttggtatta gctcaaaaaa cggcaaaata
    21901 taagaaacta ttttttcgtt tttttctcta atactcaggc cagaacgctg atttcacaac
    21961 ctctttattt cacattcaat atccacatga atcccacaaa tacaaagaac agaccataca
    22021 tgattggaga tacttctttt cctcttttag ctgctaacat ggtaattgga tagaagataa
    22081 aacctaatgc aatcccatct gaaatactat aagtcaaagg catgccgatc aagatcaaga
    22141 atgaagggat cgcaatctct aagtttcccc attcgatttg tcgcaaagat tgtgccatca
    22201 atactcctac cataatcaat gctggtgcag ttacttgtga agtaacaact gatagtaatg
    22261 gagaaaagaa caagcctaac aagaagaaaa tccctgtagt aatagctgtg atacctgaac
    22321 gtcctccaac ggcgatacca gctgatgatt ctacaaatgc accaacagga gaagtaccaa
    22381 ataaggaacc cgcaagcata gctgttgaat cagaagctaa agctttccct acacgaggca
    22441 tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc aactaaagtt cctgctgtat
    22501 caaagaaagt cacaagcaag aatgtcagca ctacgaccca catttgaaga gaattgatgt
    22561 cccctacatg ttttaatgca actaaaaatg ttggtttcaa acttggtgct gcggcaacaa
    22621 tttcagaagg cattggaatc aaacctgtga ccagacctaa aatcgttgta gctgccatcc
    22681 cgatgaagat gccacctggt acacgccgaa caagcaagat agcagttacg actaatccaa
    22741 aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa tcctactaat gtagattcat
    22801 tcgaaacgat gatcccacct tcgctcagac caaggaaagc gataaataga ccgataccgc
    22861 cagaaatcgc atatttcaaa tctgctggga tcgcatcaat gatcatttcc cgcagcttga
    22921 agattgtgat caaaataaaa atcaaagaag caacgaatac gccggctaat gctgtttccc
    22981 atggaatccc cattcctaaa caaacagaat aagcaaaaaa agcattgatc ccaagagcag
    23041 gtgccgttcc aattggataa cgggctaata cccccattaa aacacagccg atcgcactgg
    23101 ctaatgcagt cgcagtaaac actgctcctt catccattcc agaagcacct aatacagttg
    23161 gatttacaaa taagatataa gccattgaga taaatgttgt aaatcctgcc agtacctcac
    23221 gttttaccgt ggtgttcaat ttttctattt cgaagtagga aagtattttt tccttcatct
    23281 tccatccccc taaatccgaa cattataaat aaagtccgtt aaaatttcga tttattaata
    23341 aaacaacgtg ttttatcata tactaaaaca aaacataagt aaacaaaaaa ttcg
//
LOCUS       DAZUMM010000044        22725 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000044 DAZUMM010000000
VERSION     DAZUMM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.044"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..3650
                     /locus_tag="ACG8E6_001599"
     CDS             123..3650
                     /locus_tag="ACG8E6_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFS5062016.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3647..7369
                     /gene="addA"
                     /locus_tag="ACG8E6_001600"
     CDS             3647..7369
                     /gene="addA"
                     /locus_tag="ACG8E6_001600"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFS5062017.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7438..7779
                     /locus_tag="ACG8E6_001601"
     CDS             7438..7779
                     /locus_tag="ACG8E6_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS5062018.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8088..9131
                     /gene="pheS"
                     /locus_tag="ACG8E6_001602"
     CDS             8088..9131
                     /gene="pheS"
                     /locus_tag="ACG8E6_001602"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFS5062019.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9136..11556
                     /gene="pheT"
                     /locus_tag="ACG8E6_001603"
     CDS             9136..11556
                     /gene="pheT"
                     /locus_tag="ACG8E6_001603"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFS5062020.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11726..12736)
                     /gene="trpS"
                     /locus_tag="ACG8E6_001604"
     CDS             complement(11726..12736)
                     /gene="trpS"
                     /locus_tag="ACG8E6_001604"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFS5062021.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12990..13727
                     /locus_tag="ACG8E6_001605"
     CDS             12990..13727
                     /locus_tag="ACG8E6_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5062022.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13741..14556
                     /locus_tag="ACG8E6_001606"
     CDS             13741..14556
                     /locus_tag="ACG8E6_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS5062023.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14569..15216
                     /locus_tag="ACG8E6_001607"
     CDS             14569..15216
                     /locus_tag="ACG8E6_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5062024.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15232..15888
                     /locus_tag="ACG8E6_001608"
     CDS             15232..15888
                     /locus_tag="ACG8E6_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5062025.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16050..16871
                     /gene="racE"
                     /locus_tag="ACG8E6_001609"
     CDS             16050..16871
                     /gene="racE"
                     /locus_tag="ACG8E6_001609"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFS5062026.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16874..18229
                     /gene="rph"
                     /locus_tag="ACG8E6_001610"
     CDS             16874..18229
                     /gene="rph"
                     /locus_tag="ACG8E6_001610"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFS5062027.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18230..18748
                     /locus_tag="ACG8E6_001611"
     CDS             18230..18748
                     /locus_tag="ACG8E6_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5062028.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18855..19355
                     /gene="cbpB"
                     /locus_tag="ACG8E6_001612"
     CDS             18855..19355
                     /gene="cbpB"
                     /locus_tag="ACG8E6_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFS5062029.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19635..20555)
                     /locus_tag="ACG8E6_001613"
     CDS             complement(19635..20555)
                     /locus_tag="ACG8E6_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFS5062030.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20681..21733
                     /locus_tag="ACG8E6_001614"
     CDS             20681..21733
                     /locus_tag="ACG8E6_001614"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFS5062031.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21843..22598)
                     /gene="fabI"
                     /locus_tag="ACG8E6_001615"
     CDS             complement(21843..22598)
                     /gene="fabI"
                     /locus_tag="ACG8E6_001615"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFS5062032.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         7573 a         3957 c         4830 g         6365 t
ORIGIN      
        1 tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga atatagaaca
       61 aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga aggaggaaaa
      121 gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa gtcttgatcg
      181 acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc cttgttccca
      241 actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga agaagcggaa
      301 aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg gcttggtatt
      361 tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga tctgcaatga
      421 tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc ggagaaatca
      481 ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt acaggaaata
      541 ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa gaccagcaac
      601 ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg actcaacgtg
      661 cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca caagatttca
      721 acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag tatcaattac
      781 tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat cgtccttata
      841 cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt tatttccgtc
      901 tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt gcaccagagc
      961 agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca tctagccaaa
     1021 agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg gaagctgaaa
     1081 atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc agtgaagaaa
     1141 actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat ggaaatatcc
     1201 ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa caaatgatgg
     1261 aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc tattcttatc
     1321 ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg gaagcagaaa
     1381 ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg gacatcactg
     1441 aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa aaagactggc
     1501 agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat ttggaagaac
     1561 agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg aaaaaaataa
     1621 agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg atccaaagtg
     1681 gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga aacttagaag
     1741 cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat gaatatgtag
     1801 taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt acaagtggtc
     1861 tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga atcaatcgtt
     1921 tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg aatgatcaag
     1981 tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga gaagtattaa
     2041 atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt atctcttttg
     2101 agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat cttagttatg
     2161 cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt agaatcacag
     2221 aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc gaccctaatt
     2281 gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat cgattagctt
     2341 tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta gtactgaatt
     2401 cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta aatgttcctg
     2461 tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat acttcaattt
     2521 cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt ggtttgaaat
     2581 tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt tatcatgaat
     2641 cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg aatatgtccg
     2701 atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga gaacttcggt
     2761 ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta ggacagacga
     2821 ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg catcctcttc
     2881 agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt ctggaattac
     2941 cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc gatgtagctt
     3001 ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga cgttcattcg
     3061 atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg gacgtagcat
     3121 tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga tcatattatt
     3181 tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat acattgaaaa
     3241 aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta ctagatcatt
     3301 cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg aaagatgaat
     3361 accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg caactattca
     3421 tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc ggagaaatca
     3481 aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt ccattcagaa
     3541 gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg gagaaaatag
     3601 ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga atcaaattcc
     3661 tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt ttgatcaagg
     3721 agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc ttgttcgaag
     3781 agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg tgacatttac
     3841 tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag aatccgtcaa
     3901 tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt tattgcccac
     3961 agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt attattactt
     4021 aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga tcttgatgaa
     4081 ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg aacgattttt
     4141 ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga atctggtgtt
     4201 ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag aacagttaag
     4261 cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa aacaaatccg
     4321 tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga tgactcagtt
     4381 agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac aacagcagat
     4441 caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg ctggtttgga
     4501 acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag aaatttccaa
     4561 tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa gtaaaagtta
     4621 ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac cgttagtcga
     4681 agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta aacgggaaaa
     4741 aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca cagaaaaaac
     4801 gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat tcaaagaagt
     4861 aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat actggctaag
     4921 agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac aatccatcta
     4981 ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt tctcaaagaa
     5041 agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag aagtcctttc
     5101 ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa tcaattatga
     5161 cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac agtttgagcc
     5221 ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc cgacggatga
     5281 tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa ttcgtcaatt
     5341 aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc cgatagagta
     5401 caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt tagagatttt
     5461 caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc aagcgacaga
     5521 aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag atattccgct
     5581 tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag ccagcattcg
     5641 attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag catcaaacga
     5701 agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt ggcgagaact
     5761 tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga cgggatatct
     5821 agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt atgcattggt
     5881 tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt tcgtgcgttt
     5941 cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt caatagaaga
     6001 taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc cagttgtatt
     6061 tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt atgcttttga
     6121 agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt tatatgatac
     6181 gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg aagaaatgcg
     6241 taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg gttcctataa
     6301 aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg agttagtttt
     6361 tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg gttacggatt
     6421 gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt ctcttcttca
     6481 acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca ttgaacaaag
     6541 acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag aagatgaaac
     6601 tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc aagcttcttc
     6661 acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag atccagatga
     6721 tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat cccgtcaatt
     6781 tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc ggcaagtgtc
     6841 agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat tagagatgcc
     6901 aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt tagtagacga
     6961 aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa cagaacttgg
     7021 tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt ccatgctgct
     7081 tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat taatccacgg
     7141 aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt ttaaaacaga
     7201 ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa aatatcaagg
     7261 acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag tagaaaatgt
     7321 tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat tataattgag
     7381 caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt gagtatgatg
     7441 gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt ctctatttta
     7501 ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc gcaacgcttt
     7561 gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga acgcttgaag
     7621 gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa gagccgttca
     7681 gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca aatacaagtt
     7741 tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa aaatcttgta
     7801 aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt tggctaaacc
     7861 agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa tgaagttcac
     7921 tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt aagagtgcgg
     7981 tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc ttaacaggaa
     8041 tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg tctctacaag
     8101 cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca caagatttaa
     8161 atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc acagaagtat
     8221 tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt ttcgcaaatg
     8281 agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta gaagaagaag
     8341 cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt agaaaagtaa
     8401 aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat atttttgtgg
     8461 gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac aatttcgaac
     8521 ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc tatatttcag
     8581 acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg gaaaaacatg
     8641 atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc agagatacag
     8701 atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt gataaaaaca
     8761 tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg tttggggaag
     8821 atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca gtagaagtag
     8881 atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac actggatgga
     8941 tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca ggaatcgatc
     9001 cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct atgttgcggt
     9061 atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta agtcagttca
     9121 agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt agatttatcg
     9181 aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga agtagaggga
     9241 gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa ggaatgcgta
     9301 ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga agacgaactg
     9361 tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat tgttgcactt
     9421 cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg tggccaagta
     9481 tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt tataccgaaa
     9541 gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga tccagttttt
     9601 ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa tcgtgcggat
     9661 gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca gactcctaca
     9721 ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat tcaagtggaa
     9781 gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg agtaagaatc
     9841 cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg tccaatcaac
     9901 aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact acatgcattt
     9961 gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga aaatgaaaca
    10021 ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt cattactaac
    10081 ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga gatcacgaat
    10141 gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat tcgtcgtaca
    10201 gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg aatcaaccat
    10261 gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct tgcgggtggg
    10321 gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt atcagtgaag
    10381 atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa agaagtaaat
    10441 gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta ttccgtagta
    10501 gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga agttgctcgt
    10561 atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt tgctggaagc
    10621 ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg aagcggattg
    10681 agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt tgttataaat
    10741 gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt attacgaatg
    10801 aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa aaatacagag
    10861 attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg tacacatctg
    10921 ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga aaaagattgg
    10981 cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga aaacttattt
    11041 gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat gaaggaaatg
    11101 catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt tgtcggtcaa
    11161 gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc tgagtttgat
    11221 ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt gacaaaattc
    11281 ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa tcaggaatta
    11341 acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt atttgatgtc
    11401 tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct gacattcgtc
    11461 aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa agtcgaaaaa
    11521 gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag taagtaacga
    11581 tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact tcgtcacatc
    11641 tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac aggcctttac
    11701 agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt gctgatttta
    11761 cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta cgtaagattt
    11821 ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc ggtgcaaatt
    11881 cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt ccgccgcggc
    11941 ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca tccaagtagg
    12001 tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg tttggatcag
    12061 tatacatact catgactttt ttctgtaaga catcggcagg atcggagata taaataccgt
    12121 ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta cccattcctt
    12181 ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga ttgaagcttt
    12241 gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca ggtacaagat
    12301 tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag aatcctgtag
    12361 gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga ttgcgccgaa
    12421 cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat tccggaattt
    12481 gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa tagtctaaag
    12541 caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct gtcagtgctt
    12601 gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata gccactcggg
    12661 tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga tctcccgtta
    12721 aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc acttaatatc
    12781 ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga aagaggataa
    12841 aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt tctaagaaac
    12901 tttacaaatt actaaactat cagatataat gtattttatt atacatcttt aaaagaaatc
    12961 ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat cacgtggata
    13021 aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag aaaggagaag
    13081 ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc tgtatcaatg
    13141 gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg catgataaaa
    13201 agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat ttcaatttgt
    13261 acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg ttgaagcaag
    13321 accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat atgttagata
    13381 agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg gcaatcgcca
    13441 gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct gctttggatc
    13501 cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac ggtatgtcaa
    13561 tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt gttatcttca
    13621 tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc gaaaatccaa
    13681 atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag gaggaaaaag
    13741 atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt tcttgcacta
    13801 gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag caaatctacc
    13861 aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat ggatattgag
    13921 agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa aaagatcctt
    13981 ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat tccattattg
    14041 aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga acgaaaaaaa
    14101 caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt aaagaaagga
    14161 agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc agtcaaaggt
    14221 tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt agaattagaa
    14281 aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat gactacggat
    14341 aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt tggtggaacg
    14401 tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt cttgaaagct
    14461 gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat ctatcaaaaa
    14521 tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat gagcgaattg
    14581 atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt ttcgagtgtc
    14641 ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca attatcaaag
    14701 aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa cattccatta
    14761 ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa tctagatgga
    14821 ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc tgaaacagta
    14881 cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc ttctggtttt
    14941 acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat cgttatcccg
    15001 ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc aatcgtagct
    15061 ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa taccttcgat
    15121 acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc ttacttaatg
    15181 gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata catggatttt
    15241 agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa ggttactgtt
    15301 gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac aggtgtgatt
    15361 cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga tattatacgg
    15421 aatcttccat tattattgat cattttcttc acttactttg ccttacctca aatcggcatt
    15481 cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc agctatgttg
    15541 tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga agcaggatta
    15601 tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca agcatttaaa
    15661 tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac ttcattagca
    15721 gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg acagaacacc
    15781 aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt atgttacgcg
    15841 ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata gatgattctt
    15901 ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc gaaaaaaaga
    15961 gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa agtacacaat
    16021 agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat cgccctatcg
    16081 gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg aaacaattac
    16141 cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc cctagacccg
    16201 cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag caaggaatca
    16261 agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa atcaaagctg
    16321 ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca gtaaaaaaaa
    16381 cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt caagcttatg
    16441 aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct tgtcccaaat
    16501 ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa attgtggcag
    16561 aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga tgcacccatt
    16621 atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa ctgatcgatt
    16681 ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat ttgagcaatt
    16741 caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc aaacttttcg
    16801 aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat atcgaattgg
    16861 gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca aactggagct
    16921 tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg gtgatacaac
    16981 agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag aaacaggaac
    17041 aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga gaaatcgtag
    17101 agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc ttattggtcg
    17161 ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca tcgtagactg
    17221 cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg catttgtggc
    17281 attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag atccaataaa
    17341 agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg tcacagattt
    17401 ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga cagaatctgg
    17461 aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg aacagttgaa
    17521 tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc aaaaacatgc
    17581 tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa caatcatcat
    17641 tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag aagcgggata
    17701 tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa ctggcagcac
    17761 atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg atcagccagt
    17821 cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag gaatctattc
    17881 tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat tattatatga
    17941 attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt tagtattcgc
    18001 ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag tagcccgtat
    18061 accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag gtaagaaaca
    18121 aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg ctcaagcaat
    18181 gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga tgcggtattt
    18241 agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag atcgttatcg
    18301 ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt ctgacccatt
    18361 atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat tcgaacagaa
    18421 aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt tatcgaatgt
    18481 ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg atatggtgtt
    18541 ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt ttttgaatcc
    18601 tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc ttatcgaaag
    18661 tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag ttgaagagtt
    18721 ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa taaaaataag
    18781 ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa tgaaatagaa
    18841 atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa aatcaggaaa
    18901 cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca ttgtcccatg
    18961 gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga aaagatgatc
    19021 actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac ttgcaaacaa
    19081 tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca gatgttccga
    19141 caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat tcgccttttt
    19201 taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa gagctgctta
    19261 aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct ccgaaaatcg
    19321 aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa aagataatca
    19381 aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa gaggctggga
    19441 caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt tcaaattttt
    19501 gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat acgtgtttag
    19561 agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga gattcgttgc
    19621 atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt gaaaccagaa
    19681 gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata ttgttttccg
    19741 ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc cactaatcca
    19801 aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt acgatttact
    19861 cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg aatatctaaa
    19921 agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg attcgggatg
    19981 aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac agatacgacg
    20041 gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat ctgttgttcc
    20101 atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc aagaccgatt
    20161 gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc gatagcagat
    20221 aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt taaaagacta
    20281 tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg attaaaagac
    20341 ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat caaaaaaatc
    20401 agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat cccgtcccaa
    20461 aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc gccagaagag
    20521 tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct aaaatttgat
    20581 tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata actataatga
    20641 aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat gtggagaaaa
    20701 taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt cttatatcaa
    20761 aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag gagaattgac
    20821 agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg gtcagcaatg
    20881 ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct ttcgacctga
    20941 acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa tggtccctga
    21001 aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt ttgtgccgcc
    21061 ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg gtgaaaacat
    21121 tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag taggagcgta
    21181 tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc gtgctgctcc
    21241 taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac ttccaggaac
    21301 agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca cacatacgaa
    21361 aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa aattcatcac
    21421 tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc agttagctga
    21481 agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc tagatcaatt
    21541 tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg ggatttatca
    21601 caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa caaaacggtt
    21661 gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag gctggattga
    21721 agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa aaatcaggaa
    21781 gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa tgcaacaaaa
    21841 gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat tccgctggaa
    21901 agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac accttgctga
    21961 tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac acctgtgaca
    22021 gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt aggcgagaac
    22081 tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc catcatgtta
    22141 taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc gctgtttggt
    22201 gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct gatatcttga
    22261 gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc ttctttgttg
    22321 gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc gatggcattc
    22381 attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa tgcttcttcg
    22441 ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata gataatttct
    22501 gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt gttagcgacg
    22561 cccataatga cgattttttt tccttctaga aatgacataa tgactctcct ttgtttctga
    22621 taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt cttgatttct
    22681 taattattag tcaaaatgtt ttgattgtca aactattttt tctta
//
LOCUS       DAZUMM010000045        22388 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000045 DAZUMM010000000
VERSION     DAZUMM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.045"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(102..620)
                     /locus_tag="ACG8E6_001616"
     CDS             complement(102..620)
                     /locus_tag="ACG8E6_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS5062033.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(622..1239)
                     /locus_tag="ACG8E6_001617"
     CDS             complement(622..1239)
                     /locus_tag="ACG8E6_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS5062034.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1236..2090)
                     /locus_tag="ACG8E6_001618"
     CDS             complement(1236..2090)
                     /locus_tag="ACG8E6_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5062035.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIILLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2087..2998)
                     /locus_tag="ACG8E6_001619"
     CDS             complement(2087..2998)
                     /locus_tag="ACG8E6_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5062036.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3039..4373)
                     /locus_tag="ACG8E6_001620"
     CDS             complement(3039..4373)
                     /locus_tag="ACG8E6_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5062037.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4581..5411
                     /locus_tag="ACG8E6_001621"
     CDS             4581..5411
                     /locus_tag="ACG8E6_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFS5062038.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5655..5904)
                     /locus_tag="ACG8E6_001622"
                     /pseudo
     CDS             complement(5655..5904)
                     /locus_tag="ACG8E6_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6095..6289)
                     /locus_tag="ACG8E6_001623"
     CDS             complement(6095..6289)
                     /locus_tag="ACG8E6_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062039.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6794..6979)
                     /locus_tag="ACG8E6_001624"
     CDS             complement(6794..6979)
                     /locus_tag="ACG8E6_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062040.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(7829..8170)
                     /locus_tag="ACG8E6_001625"
     CDS             complement(7829..8170)
                     /locus_tag="ACG8E6_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5062041.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(8170..8400)
                     /locus_tag="ACG8E6_001626"
     CDS             complement(8170..8400)
                     /locus_tag="ACG8E6_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5062042.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            8687..9025
                     /locus_tag="ACG8E6_001627"
     CDS             8687..9025
                     /locus_tag="ACG8E6_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062043.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(9230..9792)
                     /locus_tag="ACG8E6_001628"
                     /pseudo
     CDS             complement(9230..9792)
                     /locus_tag="ACG8E6_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(10524..11105)
                     /locus_tag="ACG8E6_001629"
     CDS             complement(10524..11105)
                     /locus_tag="ACG8E6_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5062044.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(11288..11914)
                     /locus_tag="ACG8E6_001630"
     CDS             complement(11288..11914)
                     /locus_tag="ACG8E6_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS5062045.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(11936..13267)
                     /locus_tag="ACG8E6_001631"
     CDS             complement(11936..13267)
                     /locus_tag="ACG8E6_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFS5062046.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(13422..13823)
                     /locus_tag="ACG8E6_001632"
     CDS             complement(13422..13823)
                     /locus_tag="ACG8E6_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS5062047.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(13909..14115)
                     /locus_tag="ACG8E6_001633"
     CDS             complement(13909..14115)
                     /locus_tag="ACG8E6_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062048.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(14210..15712)
                     /locus_tag="ACG8E6_001634"
     CDS             complement(14210..15712)
                     /locus_tag="ACG8E6_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFS5062049.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(15730..16185)
                     /gene="argR"
                     /locus_tag="ACG8E6_001635"
     CDS             complement(15730..16185)
                     /gene="argR"
                     /locus_tag="ACG8E6_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS5062050.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(16283..16900)
                     /locus_tag="ACG8E6_001636"
     CDS             complement(16283..16900)
                     /locus_tag="ACG8E6_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS5062051.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            17121..18494
                     /locus_tag="ACG8E6_001637"
     CDS             17121..18494
                     /locus_tag="ACG8E6_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5062052.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            18569..19681
                     /locus_tag="ACG8E6_001638"
     CDS             18569..19681
                     /locus_tag="ACG8E6_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5062053.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(19753..21390)
                     /locus_tag="ACG8E6_001639"
     CDS             complement(19753..21390)
                     /locus_tag="ACG8E6_001639"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFS5062054.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(21558..22166)
                     /locus_tag="ACG8E6_001640"
     CDS             complement(21558..22166)
                     /locus_tag="ACG8E6_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5062055.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         6547 a         4546 c         3890 g         7405 t
ORIGIN      
        1 agttattaag acaaatgaat aaacaactac gctttcctcg aaacaaaaaa ataaagcaca
       61 aatgcgcatt tacgcactca tgctttattc gtacaattaa ttcagataac ggcagcgcat
      121 aggatgccaa taacacttcc taaaatagct cctcctaaaa tgtcagtagg ataatgtaaa
      181 agtaaaaaaa tcctcgaata agcgatcaaa caagcaaata aaaaggctaa tattcctgca
      241 actggactta attcgaacaa cgcagctact gataaaaagg ctagcgctgt atgtcctgaa
      301 ggaaaagaag aagtcttcgg tttacttgtg taaaggacaa gccgacgatt tttttcataa
      361 ggtcttttcc gtcttgtcgt ttttttgata aaaagcaaac caacaaaaaa atagatcaaa
      421 ctgccgataa gaatggctag accatattct ctagtatcag gtatcaagac taaaacagca
      481 gcaaggagca accaaataaa accaccatta gagatattac tgataaattt caaaaaattg
      541 atttttcgac tatccgtttt ccgttgcttg gcgaacttgt tgataatact atcatcgatt
      601 ttagcaatca aacgcaacat gttattcctc ctttttaaga accttttctt gcgtacgatg
      661 gataaaaagc aggacttgaa aataaaacaa gaaactgaaa cctaataaag caaaatagat
      721 tggtcctgca aaccagaatg cgacaagaag gatcaacgtg cctgccaaat agaaaagcgc
      781 gcttttgaaa tcattaaaaa acaagaaaaa tgcagtaatc caagaagctt ctttttgttc
      841 cagttgaagc aagattttgt aactaaagag aagcgcgctt aatagaagca aaaacaacgt
      901 agtatacatc aatgtatagc caattccccc accccaatag cggaaaatct gatagtcgat
      961 caatagaaga agaaaataaa cacttgctaa actatcttgc tttccataaa gagagaagtt
     1021 ctcacgaaac tttttccaga atagtgaacc gagctttttc tcttcatttt ttttgtaatg
     1081 ataaatcata tacagaactg ttcgacttgc agttcctaat gtgaaaacag gcaagacgca
     1141 caggaaccat agtagctgta aatggataag acgtggaatc cattcaatga tttctaagaa
     1201 ataatcattg gcttttttac caatcgatcg attcatcatc cttttgaacc tcctgtcaaa
     1261 tccatccctt caatgaaata acgttgtgca aaaaagaaca gcaaaatcgt cgggatcaag
     1321 acaagtgttg ctcctgccat cagataattc cattgtgtcg ttgcttggtt ctgaaaaact
     1381 tgcaagccaa tctgtaacgt gtatttttct tgatcttgga tatataacag aggacctaag
     1441 aaatcattcc aagctgcgtt gaaggaattg atcgcaatgg taatcaaggc aggtttcgtc
     1501 aatggaagca tcaaatgact ccaaatatac aaatgattcg ctccgtcgat ttcagcagct
     1561 tcgatcaatt cattattgat cgataagtaa aattgacgca tcagaaatat attgaacgca
     1621 ttgccgaaga acgatggaac gatcaggggc aaataagtac ctacccagcc gatcttggag
     1681 aataatacat attgagggat catcgttaca aaccccggga tcatcatcgt agaaagaacc
     1741 aaagcaaaca tcagcttttt cccaggaaaa tcaagcttcg caaatccata agcaataaaa
     1801 gaatttgaaa ggacattacc gatcactact agcacagtga tgaataatgt atttcttgca
     1861 tagcgccaaa aaggaaaggc ggcaatcgta tccagatagt tttcccatac gattttttct
     1921 gggaaaaagg tcggaggata acgaacgatt tcttccattg gtttcaatga agtaaacacc
     1981 atccaaagca atagaatgat caaaatgatt cctattactg tcatgaggac ataacttagt
     2041 attttttttg tttgccgatt cacactggtc tttttgaaat aatccatcat ttgccgtcac
     2101 ctccagaata atagacccac ttaggtgcaa gcttcaaatt gacaaacgtc agaaccaaca
     2161 cgatcagaaa caggatcatt gacatagcca tcgcataacc catatcaaaa gctacgaacg
     2221 ccttgttcca catatgcaga ttataaaaaa gcaaagaatt tgcaggtccg cctgttccat
     2281 ttttcgtcat gacataagct tcttgaaaaa tttggaaaga accgatcgta gaggtgatca
     2341 catcgtaaaa aataatagga gtgattaatg gcaaagtaat acttttgaac tttccccaaa
     2401 cgcctgctcc atcgatttct gctgattcat ataaagatct aggaacattt tgtaatgtag
     2461 ctaagtaaag tagcattgct cctccagaac tccatatttt catcaaaatc aacgcaggtt
     2521 tcgtccattg tggatcaaac agccaagctg gtccagggat atggaaccag cccagaaatg
     2581 tgttgatcaa tcctgcagat ggagaaagca gctgcatcca taagatgtag actgctactc
     2641 ctgataagac tgccggtaaa tagaaagctg tacggaatac actgattcct ttgatttttt
     2701 gattgagtac tgtggctaaa aacacactga aaatcgtcgt cagcggtaca ttgaagatga
     2761 cgaaataaac ggtgttgtat aacgatttcc aaaataaatc atcgtgaacg aacattcttt
     2821 tgaaatttgc tagaccaatg aaattcatct tggatgtcaa attataatcg gtgaaactac
     2881 caataactga aaaaagcata ggccctagca taaataccaa gaacccgata ataaaaggcg
     2941 agataaataa ataggcccat cttgcttctt ttttctttcg gatagataaa cgcttcaaat
     3001 tttcttgcct cctctgtacc tagacagtca aaaatggatt attgattttg cttgactaag
     3061 ttttctactg ctttttgtgc atctgccaat ccttcttttg gtgattttgt tcctaaaaga
     3121 atctggtcaa tctgttcatt cactaatgtc gtgtaatccg gagcatctaa aggaactggt
     3181 gtgatcaccg tatttttaaa gttttcgtcc gccatttgat agatcatttt tcctttatcc
     3241 gttaggttgt cattattcac taaattctca ttggctgtct tgctcgccat gatatcaaaa
     3301 cttttttcgc cgaatttttc ttgtacttct ggagaagaaa gatatttgat gaattcataa
     3361 gaggcctcag gatcttttgc tccgtatggt acttccaaga cgaatccgcc accccacgac
     3421 caatggcctg aaccttcttc tttttctggg agctgcatca cgccaaaatc aaaatcatcc
     3481 ggtgcatttt ccatcaaact agagtaataa ttgatatttt gggcacgcat ggctactaat
     3541 ccggaaataa atggatcaga tacaccagac ccaaattcgg cttctaattt attgatcgta
     3601 tttttcccat aataatcttg ccagtcttgg atccactcca aggcttccac tttattttta
     3661 gtatcaatct tgactttttc atctttgtca aaccaactag tcccgttatc tgcgtttagc
     3721 gcccatacat cagcccctaa attccataat ggataaaaac cgatgcgtgt aaattctttt
     3781 ccttcttttt tatctagttt acgtgctgtt tcttctaatt catcccaagt tgtcggtaga
     3841 tcttgttcac tgattccagc ttctttaaat aactttttat tataaaaaat gacttgtgta
     3901 tctgtattga atgggatcgc ataaggatca ccttcatatt ctactgtatc ccaaagctgc
     3961 ggataaaaat ccttagaagt gtctttttct acatatttgg agagattagt tgcttgctgt
     4021 gcttctgctc tttgggcaac ggaatttatg tcctgtacaa taacatcggg tgcatttcca
     4081 gctgttattg cagataaaga tttagtccag atgtcacccc atggctgata cttataattt
     4141 acatggatct cgttttgtga ttgattgaaa tcctcaatga tttcttcgat agcttctttt
     4201 cgtgcaccgg atccccaaaa ggaccagaaa tcaatatttt ttcttccatc acttgttttt
     4261 tcagaagaat ttgctgcact tgaactggca tcgcttgcat tacttgatcc gcatcctcct
     4321 aataaaaaca atgctgaact tgccagcaac cctgtaaaca ataatttttt catattttac
     4381 ctccaaattt taatgtaacc gatgccggtt ttaatataac gatatcattt tgttctgaaa
     4441 ataacaattt ataaggtatg attctaaaaa aattattaat gataccaata ctttatataa
     4501 ttcgcgagcg ctttctttga attttttaat ctttcgtagc agaatgagaa tgtattaaaa
     4561 atagattagg aggaattttg atgtttagac accagaaaga attgcagttt gaagcaaaac
     4621 cagatcgtcc gaatccatta atcgcgaaat atctgcagga gctgatcggc ggccaatatg
     4681 gcgagatgag tgtagctatg cagtacctgt tccaaggctg gagctgccga ggagaagaaa
     4741 aatataaaga tatgctgatg gacattgcaa cagaagaaat cggccacgtg gaaatgttgg
     4801 cgacaatggt tgctcgttta ttagagggtg cgcctgttga agatcaagaa gcagcgatgc
     4861 aaaaagatcc aacaatcggt gccattatct ctggaatgaa tccacagcac gcgatcgtag
     4921 ctggcttagg acctcgtcct gctgacagcg tgggcaatcc atggagcggt gcctatatca
     4981 ttgcgagtgg caacttgtta gcagacttta gagctaattt aaatgccgaa tcacaaggaa
     5041 gacttcaagt agcacgtatt tacgaaatga tcgatgatcg tggcgtaaaa gatatgctgt
     5101 ctgttctcct tgcaagagat agctatcatc aaaatctatg ggaatgtgca gtgaaagaat
     5161 tagaagaaaa agaaggttca atcttagtcc caagtacttt cccgcgtgac aaagaacgaa
     5221 aagacattgc ttatgacttc tataacttct cagagggaga ccaaagtagt gaaggttctt
     5281 gggctagtgg caaagcactg gatggcgaag gcgaatatca atggttgaaa gaacctaaga
     5341 tcgaaggcgc tgcaccaaaa ttagaaccag taacaccaaa aatgtatggg acacctccat
     5401 tcaagaaata aatatctacc cgtagaaaca cccgtttttt gttccacacg ctttgtgagc
     5461 aaaactgact aaagtcatta atcataacca cccttcaaaa gggtggtttt ctctagagct
     5521 ataagctcaa atcaccggcc agcgcctaaa gacgctggct ttcatatttt atgttcaagc
     5581 catattgcct ttgccagttt tgcccacctc ttaaaaggtt tatgtactac tccttagccc
     5641 ttaaaaggat cgtcttactc tcgtacactc agcttatcta acgcactatc atgtttttct
     5701 tgttcttgaa tgtacttttt gatggtcgct tcattttgtc ctacttttgt cacatagtat
     5761 ccttccaccc aaaagtgtcg atacccaaat ttatatttta gatttgcaca atctcttgat
     5821 gatctcctga atattggctc gatattgatt ataaattatt tttcttctat actttggtgt
     5881 gaatacaatg tggtatttac acatctactt tgtacgggct aaactatttc cctttattgc
     5941 tccacccgca tagcaagtgg ttttgtattt caatcgctcg gcgcgctcaa ttaggtctaa
     6001 agacaataaa tctaaaaacc cgctgcttca ctgacagaaa caacgggttt tcagaaaaaa
     6061 atgaaatgaa aaagatctct tacggcaatc tattttattt ttttgatttt ttataaatgg
     6121 aaaatgcttc aagaagtcca agaaataaag ccgtataaaa acaagaacct atgaaatctt
     6181 tatttattag atattcaaca gaaatcccga taacactagc caacaatata tttatcagaa
     6241 gtgtaaaata aggatgcttt ttatagaata gaactagtgt tgttttcata gactctcctt
     6301 tcttcatatt tccaattaat cacaaattca cggcattact agaaaaatta aaggggcgca
     6361 gaagtggaaa aatactcttc gactttccct acaaaccagg ccatcttgtg tttaataact
     6421 ataccaaatc taaggtacct ctaaatctaa attccatttg tggcggtgac ctccaatttt
     6481 taactcttct atctcccatc cagtatacat aaccttgata cttcgaccac ttatatatcg
     6541 aacacacaaa tacctatcca ttgcaggagt actacctaac ttagttgcta cttgcatcta
     6601 aataacagct gaaaagatag gacttactct tagtaaagta tctgaactat taatttttaa
     6661 actatcattt ttatcctcaa ctacatgcac atctgttgca acttcacttt ctgtctcatc
     6721 cagataaatg attaaggaat cgggaattgc aatatcttca gctgacgctg gaatagttga
     6781 gctaaattag tcatcatatc tttattataa aataaccaac agcaaaaaat acaatgaaaa
     6841 acaaacttgg aactaatgta tcattaataa atgttgaaaa attcagcaac tcattactgt
     6901 atgattttag cagcccaacc aacatatacc ctataaaagc taataagatg gttaggaatc
     6961 ctttagaatt tttactcatt catagacact tccttaaata acttttatta tagaaggtat
     7021 tgtaaataat aatattgaca aaaagtgact caaaataaaa aaaaacttac attatactgg
     7081 aatgaactgt attgatagtg tacctaaaag ctctactgac ataagatcgt gtaatcggta
     7141 aagtaatttt attaattata cgggatgtaa tcaattagag aataaactaa tcataaaagg
     7201 tacttttaca ttcataattg tcgctttgag attctacttt ggttcactat ctgacccaat
     7261 tgcacatttt cgatcattac aagtattggt gtcgttagaa caactaaata aaaactagcg
     7321 gtagattttt cagaatctat gctaatttct gtatggatgt tccagtgatg gcaaatttct
     7381 cttttcattg tttttcttgt cgcatgatat gcctttttgt gttattcagt taaactatag
     7441 aaattctttc ctagacagat cttgatccca aattttacgt ttttgagcat cgatatagtt
     7501 taaaatgaca ttactcatat ctaaagagtt tagatttttt aggactaaaa ctgtcagttg
     7561 ttctttttct tctttctgtt aaagtattgt cgcgactttt cgtatacgtt aagatatgtc
     7621 gtgaaaaaac aagttttgtg acacaaaaaa acgaatgact tgaggaaatc acctcaatca
     7681 ttcgtttaaa atgctatata ttctttagtt taagatacgg aaattaatgc catcgaccac
     7741 actagtagaa tgaacgacat ttacttggcc tttttttaat ttcgttatgt ttagcatata
     7801 aaaatattat agagtttact tacaatgctt aaaacatata ctgtataatc tgatcaattt
     7861 tagccatgtc ttgcaatggt aagttttcta tttttttgag ttttcgttcc ttaaaatcaa
     7921 gagattttaa ttgtgtaata attacttgtc cttttatatc tagctcttca ggtaatgagt
     7981 aacgtgtcgg taaattcttt atagtggaag taattggaca tatgaccgaa aacattgttt
     8041 tacggttaaa ttcataacga gagacgacta gtcctggtcg tcttttttga atttcttttc
     8101 ctacggaggg atcaaaatca atccatacaa tatcaccttt tttaggaata taaattcttt
     8161 cttctgacac tataaaattt ctctcccttc aggagttaaa tcttcccatt cgtctttctc
     8221 ataatactcg gcctcttccc caccactaaa tggatcttca atttttggaa ctaacgtaat
     8281 tgttccatca tcagaataca caacgatata ttcttggttt tctgaaggtt tctttccgtt
     8341 atcagaaggt aaagttacca ctacagaact tccttgtaat ctagattttg taacaagcat
     8401 tatcatcact cccttaataa aagtatacta tataacacta agtaacgcaa aattcccctt
     8461 tgtaaactat ctagaaaatt gttttgttta aagtatatct tattggatat aaatagaaat
     8521 aaattgtagc cagttcattt tttctttaac taaaaaaata atccaagaaa cattgaacct
     8581 atggatccaa aagctaccca aatactatag ctaacccccc gctaccttca attttgcaga
     8641 taaaacgctt tgggagaaaa tttcaaagac aacaggccat cctatcatgc ccgattataa
     8701 acaaaaagct ggctattcct tttgctctga caaatttcaa ttgggaaagt tcacgaaact
     8761 agcaggtgaa gaagtttcaa ctgttcggat caaagaatgc ccgcttcaaa tagaaacatt
     8821 ggttcaacga atttcaacta gacaagactt tgctattgta gaacgtgaga tcgtagctat
     8881 ttttgtagaa gaagggattt tacatgatcc aactcatatc aatgtggata aatggcaacc
     8941 tcttatctat aaattcagag aatatacaac tgccaaccag tcgttaactt tgaattttag
     9001 attccaagaa ttcaaagagt cataatgata aacatcgtta ggtataaata aaaaagactc
     9061 gcagcacgag taaaaaacaa atagaagcta tacattagaa aggtcgactg tgaccctata
     9121 gatgcagtat atgcagctta tgagcatttc ttctgttctc tttgtttttg ggatctgtgc
     9181 tgaagtcttt ttcgtttttt gccgctcttt ctttgaaaaa tcgattatgt tataccttct
     9241 ccattactaa ttcaggcatc tgctgagtaa tgatttttcg cgcaaaagaa atatctgtta
     9301 cccaaggttc gatttgttct tcagtcaaga caagcggcat tcggtcatgg attggtgcga
     9361 ctgtgcggtt aggttcagta gtcattatga ttgattctgc cattttttca tcctgatggc
     9421 tacgataaaa accgccaata tacactattt tttccttatt tgaaaacata aatttgtctt
     9481 tttcttcatt ccactcattg ggaaaacaca acgattttgc aaaaatggtt gatgaaaggt
     9541 ctttttttct tctactgtct ctgcacgggc attaatcatc aattgtcctt ttttaaatcc
     9601 ggtaaatccc cacttcatga tttccggagt gaggatgttt tcttcctctt ttcttaacgt
     9661 cacaacaaga tttgacggaa aaacttctcc agctgctaat tctgctgcct gcttcggttg
     9721 tttcgtctgg gcttctcgat aataattctg aaggagctct gactctagat caaaaaaata
     9781 acgaccacac atcctaatcc ctcccaatct ttttttctat catacactgc aacaagaagt
     9841 tcgcaccaac aaaacgcttt caatttttgt agaactaaaa aagttgtgac aagtgacaaa
     9901 ctctccagtg aatggtattc tcctgccaac tcctcggtca caagtcacaa tattcaacaa
     9961 acatgagaag ctgctttttg aaattgttcc ttgtgactcg gagtgaaacg gcaggcttac
    10021 gacctctggg taaacggtgt tcaaaagcca gtttctcggt attaagccaa atttatgcaa
    10081 aaatgtagaa agctattttc acaaaatttt tcttaatact cgaaactaag cgactttttc
    10141 acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa
    10201 cgaaaaatat gagcagctat ttttatttgg ctgttcttat tacttgatgc agggcagctt
    10261 tttcacaacc tctccactaa acgatgttca atcagctaac cttcggtatt agctcaaaaa
    10321 acggcaaaat atgaggaact attttgccgt ttgtttctct aatactcagg tcaaagcgct
    10381 gatttcacaa cctctaatac acggtgttcc agaagtagct tcttcgaaaa ataagccgaa
    10441 atttcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    10501 acttctgtca caacctctaa ttattaaaga accttttgcg aagttaacaa tctttctttg
    10561 atcaattgaa aattctgcct aagtagtgat agatcctgtg tgactttcaa caataagata
    10621 cttccctgga tttcagcaat caacgctttg gcttgacggt ctgcttcttc atcggacagt
    10681 ttttcttcag aaagtgccag gatattttgt ttcgtatact tgacccattg attgactaat
    10741 tctagtagca acagacggta ttcttcatcc actgtactca actcaataat caaattacct
    10801 acaggacagc catgataagt gatctgtgtc tcatgatcag cgaaaatcca gtccaacatt
    10861 tcttctaatg cttcttctgg ctgttttcca gcatttaaga tgtcttcgaa caattctgtc
    10921 tgccatacgt ccatatgcgc atggatcacc gctagacaaa cttctttttt tgaagaaaag
    10981 taataataaa attgaccttt tcctgtatca gaagctgcta gaatatctcg gattgacgtt
    11041 tcatgatagc cttttttata aataagatct ttggtcgctt ccaagatctt ttcctttgtt
    11101 ttcaatgata ttctcctttt tcatttctca tagtcctata ttatacagta aattcatcat
    11161 tttttcttct taatcgttta aaaaagactg tgccaaatta tctgacacag ccctcactat
    11221 aaaactgttc tcatatagct agatgttcat tgattcaaga actgcgagga caaaagcaat
    11281 tcctatctta tgatagttgc tctaatactt cctctgttgt catcacttga tctgcaataa
    11341 agccaaagtt cgtcaacgct gcatccattg cttcttggcc aggagcacca attgcatctg
    11401 tcgctaccac aacattgaat ccactctcta ccaattcacg catatgggaa tcgctacaca
    11461 agtttccgtt cattccacca agaattactg tgtcgattcc attttttctc aattggagcg
    11521 ccaaatcatt gctttctggt ccaaagattt tatgaggaga gctgatgatc gtattttcat
    11581 ccaaataagg tttcaattct tcgataaaat cggctcctga accttcttcc actgattcat
    11641 attgactttt tctagcaaac atctgctgct caagcatcat atgttctcct gcaccgccaa
    11701 attgccattt atggtcatgt gggtaaaaat aatgaggaga aataaagagt ttgtatcctt
    11761 tttcttttgc ttgttttacc atttcaatca tattttcgat cgtattgtat ttttctaaga
    11821 tatcttttgt caaaccaaag ccaccgcctg ctggtttcaa gaactcgact tgcgggtcag
    11881 tgagaactac tgctgtattt ttattattga acatggtgga ttctccattc gttatttatt
    11941 ttaatacatc atttagagct tcactcattg tcggatgaga atagatttga tctctcaata
    12001 acgtataagg aagttttcca ttcatagcta gcgaaatcaa gttgatgact tcatgagaat
    12061 cttccgcata gatacttgcc cctagaatca agtcagtttc agggtcgact aaaactttga
    12121 atacaccttt aggatcttct aatacttgtg ctttagggac accgcttgtc atatacttga
    12181 ataatttgta attgacgcct tgtgctttgg cttgtttttc tgttaaacct acattggata
    12241 aagtcggagt gataaataca ctgtaaggca cagctttccg gttattcgtt gttcgtttat
    12301 tctcgccctt caactgatcc ataatgattc gataatcatc tagagagata taagtgaact
    12361 gcaatccgcc tttgacgtct ccaatcgccc aaatgttgtc tgcatctgta cgaaggtatt
    12421 catccacagc gattgctccg cgatctgtta gtttaacatc tgtattttct aatccaagtg
    12481 atgctgtact tggttttctt ccagttgcag ccaaaacttt tgacgcttgg attgttactt
    12541 cttggccatc ttctgtaaag gtgacggcag ctgttgattc ttggtctgct acttgatcta
    12601 cagatacacc tagatggaat tcgattcccg catttgtcat gtcttctaaa atcatttgcg
    12661 cgatatcttc atcttcacgt ggaagaaatt cagaatgggc atcaagaacg acaactttag
    12721 atccgtattc attgaacatt gatgcaaatt ccaagccaat atatccagcc ccgatgatca
    12781 ctaatttttc cggtaattgt tcctgatcca tcgcttgcgt agaatcaatc aagtagcgac
    12841 ttgtattcaa tcctttgatt ggtaaaatga ttggttcaga accagtattg atgaaaatcc
    12901 gttcgccttc aacaatcact ttttcaccat tattcgtcac ttcgatttga tggttagaaa
    12961 gaaaacgtgc tgtaccgtcc atcactgtcc cagttgcctc atcgctgatc atatgataat
    13021 ttttggcgcg aagcatgccc gtcaattttt ctttccgagc aacagcctct gtaaagtcga
    13081 ttcctctttc tccattgaaa atcaatgatt tagaaggaat acagccgatg ttgatacatg
    13141 ttccgccata catacgtgtt gacttttcaa cgactaaaac cgattcacct tttgaagcta
    13201 acgccttcgc taaagtctta ccgccctttc caaaaccgat cacaatattt tgatattttt
    13261 ccatcatttt tccctcgctt cttttttacc taccgattgg tacaaaaata tgataaccga
    13321 taattgtcta ttctccaaat catctgcttt atttgtcaaa aaaaaccaga caaaaagcgg
    13381 tcctttcacg tttttagtct ggttcgattt cctttattct tctaaacaat cgttacttct
    13441 acatcgatat ttcctctagt tgctttggag taagggcaga attcatgcgc atcttctgca
    13501 attttttggg cttcttcttc gctgatgcca tcgatatgca cttctaatac tacaccgatc
    13561 tgatagccgc catcatctaa attataaaga gagacacgtg cttttactgt ggactctgct
    13621 gtcattcctg cttctttgat catatgttgt accgccccat tgaaacaaga agcataacct
    13681 gctgcaaata attgttctgg atttgttcct tctgcatgga ttcctggagg agtgattttc
    13741 atatgcatcg agccatttgg tgcctcgact tctccattgc gcccgccatg gttgatcatt
    13801 gctgtttcgt atgctttttt cattgtactg ctcctttcga ttgaccattt gctacttggc
    13861 tataattata gtgtatgaca agtaaggcgc tttctacaaa aattctgctt attcgaatac
    13921 cagaatgccg tcttctacta tttttccaaa aaacaaaaaa atactactcg tgaacaacag
    13981 cgtcatattt gttccaaaat aaatagcaat cttttctaca taatctttta tatgattgat
    14041 tttaagatac atatatacca gaaagttgac cccataaacc aatactgtta aaaaaataac
    14101 catattcgtt accatttttt cctccttttg ctggtctccc taccagcact cgatttaacc
    14161 ataccacttt ttttgccgtc caagcaacta gcctttcaaa aagactggtt tattctgctg
    14221 ttaatgattc aagatcagaa aaatgtccga ataattttgc taattttact aacgccaact
    14281 tgttctttcc gaattcatca ttttctatct gtaatactag taacggctca tgtaagctta
    14341 aacggatcaa aaaccagccg tttccgtatg gctcttctag atcatatcga ataccttctt
    14401 cattttccgg gtcgaagttc atcccttttt cttcttccaa cagtcgcttc atttcttcta
    14461 atatcttgtt tccttgttcg cgtggttcat cgctgaacaa ttttaatcgg atttcttgta
    14521 cttctacggg ctgtttcaac tctttgattt gttcatctaa tgtgatacct tttcttttta
    14581 attctggcaa aagcatcagg atctttgcga tgacatatgc accatcatcc agaaaatagt
    14641 tttccttgaa tgcagcgtgt ccgcttgttt cgatggctag ctgtgtatct ttcccaagac
    14701 ggttaagttc taatgcttta ttgattacat ttcgatagcc ggaaatataa cgaacttgga
    14761 tgcctccttt ttcttcaatg aatctttgta gatgagaaga agtaggggaa ttcgtcacga
    14821 ttcttgttcc tggatgctct tttaatacga ttgtacttaa tactgcgatc aaattatttc
    14881 gattgatcac tgttccagaa gcactgacaa ctgcagaacg atccacatct gtatcgaaaa
    14941 tgatcccaag atctgcttca tttgctaaaa cagcctgctt gatactttcc atcgcttctt
    15001 tattatcagg atttgggata tgattaggaa atcggccatc tggttccaaa aattgggaac
    15061 ctgttgaatc cgcacctaag ctattcaata ctttttcagc aaagaatcca ccagcaccat
    15121 ttccggcatc aacaatgatt ttccaaccat ttagtggttt gctttctagt gttccaatcc
    15181 cctttttaat ttttgtgact aaatcttgcg cataaggtgt caaaaggtct gctttttcta
    15241 cgcttccagt ttgttcgctt tttatttttt gctgctgtga taggataaaa gcaatatctt
    15301 ctttttctgc tcctccttct gaggtaaaga attttattcc attataataa tagggaagat
    15361 ggctagcagt cagcataacc gcagcatcgc acgagaactg ttcaaattga gtggacataa
    15421 acatagcagg cgtagtagct aattcgaaat cgatgacatg tatcccttgt tctttcagac
    15481 tttggatcaa cgcctctttc agtgctggtc cgcttatcct actgtcatga ccaatcccaa
    15541 tcgtccattt tttatttgga tcggttgatt tcttcttttt ctttatccag tttgctagtc
    15601 cccagccgat cttttttacc atgtctgtcg tcaagtttac ttcatatcct tcaactggta
    15661 atgcgattcc tcgaatatcc gatccatttt gcagcgcttt catatctttc atataaaagt
    15721 cctcctcatt tattccctca ttttttccag cttttctgca aatcgctgtg cttcttcttc
    15781 tgagcggcaa ataataatga atgtatctac accggccatc gttccgacaa cttcagggta
    15841 agccacttca tctagccaat tcgttaccac atcggcacca tttcgttcag tcaatacgat
    15901 aaccatgaac tgaacagact ggatacgcaa aacctcgcgt ctaattgctg tacgcaaacg
    15961 ttcttcattt aaagaaacag atggctggtt aaataatgta tagcgtacta ttttgttttc
    16021 attttgactt tttactaact tcatttctcg aatgtctcga gaaatcgttg cttgagtcac
    16081 atcggcgcct gcttcatgca gccgcgcaag cagctcttcc tgtgtcccaa taggttgttc
    16141 tgtaatgatc tggcggatca gtgcttgtcg ctgtttcttt ttcatattag acacctctca
    16201 atgttgatta tagcaaaaga taagtaatat ttcttcatat tttggtattt tttactttta
    16261 ttttttgttt tcattcaggt tgctaacctt tttctatttt aacacgcaaa atcgtcttca
    16321 ttttttcagg tgctgatttt cttggatcgc tgatgtaaat ctctttgtgt tctttagaag
    16381 ctctttcata gccttctttt gcgatgaatg cttctaattt ctcaaacgtt gctggttctt
    16441 cggcataggg cccgacgtgt aagatttggg cacaaagacc ttcttccatc tttttgaata
    16501 ctacttgttc tttcaagtct tccggaagtt tgctagaacg ctgaatggct tcttgtgcta
    16561 tcgcttctgt aacaaagtca ggctgtcgga tcatcaattg ataagaaaaa tgttcttttt
    16621 tcatcactgg ttcatgcaaa tacttttctt gcagtcccca ttgtccttcc agtggataga
    16681 cagtatacat ctgataatca ggaatcagcc aatttttttt aggcgacatg cgtattgcat
    16741 agctgactgc atatagacag gctactcttc gtttaaagtc ttcctgattc gggtcgccga
    16801 tccctctgat gctaaagaat gattttttag gaacctctag tatttgcggg ctttttttca
    16861 tactatagct cttgtcttta cgccattcaa ttttttccat ttttcttcct ccgcgaaatt
    16921 ccatttagtt atttattatc atacctcttt ttgagtgact tggctaatcg tgttgacact
    16981 atttttcagt ttgtgctaga ataagtgaag taaataaatc gtacagataa cacgtttcgg
    17041 cgtgtcgaga gagaggcggt ttctattttt ataatagaaa tcactctctt tttttacgaa
    17101 attttttaga ggtgatgtaa atgacaaaac aaaaacagct gcttttagta cctgcagtaa
    17161 ttgcaacagg gatgatgtct tttgcgggcg tattgatcga gacggccatg aatgtaactt
    17221 tcccaactct tatcagacag tttgatttaa cgactgcaca agtccaatgg gtaacaacga
    17281 tttatctttt gatgatttcg atcattgttc cgctgtctac ttatttgaat cgcaatttta
    17341 gtctcaaaaa actcttttta gcttcaaatc tgcttttctt ggcaggtata atgatcgatt
    17401 tcttctctcc taccttcatg gtcctattgt ttggtcgatt gctccaaggt gcagcgacag
    17461 gtatcgctct tccgttgatg ttccatatta tcttgacatt tgcccccctc aatcgacgag
    17521 gcgcgatgat gggtgtcggt actttgacaa cggccattgc tccagcaatt ggccccactt
    17581 atggaggcat tatgacttca cattttacat ggaatcatat cttcctgttt cttgtgcccg
    17641 ttttgattct ttctttgatt atcggctggt ttgctattcc cgagatggca gtacaaaaaa
    17701 gtggttattt ggattggata agcgttttag gaatttgtct tttgtttagt ggaagtctaa
    17761 catttttgag tacgttggga acagtttcca gctggattgc tttaatcatc ggcggtattg
    17821 gctggttcct tttttactat cgaagtaaaa aagtggagca tccactgatc cgactgtcta
    17881 ttctttcaaa taaaacattc cgcttgtttt tgattggctt cttagtttat cagtttttat
    17941 tacttggtgt atcttttgtc ttgccgaatt tccttcagat cgttcaagga agtagtcctt
    18001 ttgtcgctgg attagcgatg cttcccggcg cagcggttgg tgctttgctt tctccttttt
    18061 ccgggaaaat gctggatcaa tatggtccga agaagccaat ttttgctggg ctgattcttt
    18121 ctttgattgg ttggctagca ttattcctac tcataggaca cgcttcagtg ggtgtattga
    18181 ttgcttgtca tgtgttttat atgatcggta tcggattatc ttacagcaat atgatgacca
    18241 ccggaatgaa tgcactgaat gaagaactac aaggagacgg gaacgcaatg ttcaatacct
    18301 tgcagcagtt ttctggcgct gtggctactt cattagtagc ggtcattatc aattacgtcc
    18361 agcatcatac ttcccataac tatgaagtgt ctactacatt aggatcaaaa gcagcattag
    18421 gcgtcttgtt gctgttgctt ctcgtaagct ttgctcgatt cgcctattat ctgttctttg
    18481 ctaaaaaagc gtagattcca tatacggcag gtgttgcatc tgccgttttt ttgtgtgaaa
    18541 atggttgttt gtaaaaggag gcttttttat ggaaaaaatt gccattatcg gtggcgggat
    18601 cattggtatg accctagcca gttacttaga tacatcaaaa ttcaatgttg ctttatttga
    18661 tgaagggaca ggacaggcaa ctaaagctag cgctgggatt atctcccctt ggttgtcgaa
    18721 acgcagaaac aaacaatggt atcgtctggc taaagacggc gctgcttttt tccctaaact
    18781 tataaaagat ttccaacttg atcaaacgat ttacgcacaa tcagggacac ttttgcttcg
    18841 acctgaaaaa gatttagctg aattggctgt tcttgccgag aaacgaaaag aagaagcgcc
    18901 agaaattgga aacatcgaat ggctttcacc tgaacagaca tctcaagcac ttccgttatt
    18961 gaaaccagca gtttcactgc gtatcagtgg tggtggtcgt ctggatggaa aagcatattt
    19021 agaacatctt ggtcagcgtt tagcacaaaa acaaatggag cagatccagg caaaagtcac
    19081 tttgcataag aagagcgatt cttggctcat caagggaccg tttggagaat acacagctga
    19141 tcgtgtaatt ttgacacctg gcccggcact taagccttta ttacaagaat taggctatgc
    19201 tgcagatata cgtccgcaaa aagggcagct cttagcattt cagacatctt ataagatgag
    19261 tcaagactgg cctgttgcta tgcttgacgg agaagctgat ttcattcctt tcatggatgg
    19321 aaagatcctt ttaggtgcta cccatgaaaa cgatcaaggc tgggatttgg ctcctactag
    19381 cgaagcttat aagggattga ctaagagtac agaacagttc ttgactgatc cagatcagat
    19441 aaaaaactgg ccatacgagt tccgtgtagg tacccgtgct tatacgtcag atttttcccc
    19501 atttttcggc gtactgccag atgatccaag cttactggct gctagcggtc taggctcttc
    19561 tggcttgacc acaggtcctt atatcggcta tctattagcc aacttcttaa atactggtca
    19621 ttggcctacg gaagattatc aaaaggacat ctccacctat attacgaaaa atccatcata
    19681 gaaaaacagc tttttcaagt aaggatcccg agcgatattg cgcatggatt acttcttgaa
    19741 aaagctgtta ttttagttca atttctgaat caaattctcc aaactttctt ttgcttcatc
    19801 tggcaagaga tcttttccta tctgagcttc aatgaatcgt aaacgaatat caagtcgttg
    19861 tttcaccaga tttttatagg acgtatcctc aaaagaaaca ggaaaatctg gtataggtaa
    19921 atagtggatg tcttcaaatg gaccaaacga cttgaattct gccgtttgtt gcacgattct
    19981 ttcaacgatt gacaatgtcg tttcaggttt gattttttta tcatcgaaat atccagtatt
    20041 caggacatag caggctgttt gctgtttcaa aaacaactct cgaaaagctt gatagtcttc
    20101 cctaagagga tagacacgga aaggattagc aaatggttca atgactagct catctagctg
    20161 tctttttgta gtgatatttt ccgcgtctga tcgttttgtt gctaatgttg caccaaaaga
    20221 cgctgctaaa actgggtcat tgatcttgat aattggcgga agcgaatcat ctttcatgat
    20281 ccaaaaaaca gcatcgatct tttcgctaag atgatcgact cggtcgggtg tcgcataacg
    20341 tgatttgact gtccgcccat ttccgtttcg tatatctcct gttactagga ctttttctcc
    20401 tttacttgta catgtcacgc ccacattctg acaagtaagg aaagaagaaa tggccggatc
    20461 atcagacgga tagtcttgtg ttttatcaaa gtaagaaggt tccaaggcag tagcaaaacc
    20521 attttttcga gaaataacga aagcatcgtc atgcaagatc gaaacagagt gaccttccgt
    20581 atgttttgct aaggtaatcg ttgattttcc agaaccggac aacccaaata cagccatcgt
    20641 ataggtctgg tcattactag catattgttt catcccaccg tggcaggcaa taaagccatt
    20701 gcgatttccc aatgcccaag caagcgtcaa agtcgctttt tttaattctc caaagtaacg
    20761 taatcctagc acagccgctg tattatggag agcatcaaac aaaatgagtc catttggaaa
    20821 tgtttcatgt gtccattcgg gatcacaaaa gatataaaga tctccctcat caaaggaaac
    20881 agacttttga taagtctcta agatcttcga attcgctggc tgaaaattca gcaaataaga
    20941 atacaaattg ttctcaaatc cttctggaat cagcaaatga ctttgcacag aaaattcttc
    21001 agataggcca acgattactt ttgtatggta aaactggcgc ttgcgacttt gatacacagc
    21061 ttctcttaaa attttctcgt atttgctttg ttccttgtct aagtcctcaa agatccgccg
    21121 agcacttgct gttcttccca caatgcttcc atcattgaat actagtgtcc taacatcagc
    21181 tggtaaatct agttcttcag catgtgagat tgttaaatct gtgatgatcg ttcctggact
    21241 cccttttgct agttgataag ctgtcttcct atcaaaaact tcatgaacat tattaccgta
    21301 aaatgtcgtt tctacaatcg atcgaatact ggataacagt ggatttccac ttgtgatttc
    21361 gtttaattcg agactctctt ttgtactcat ggctcctcct cattccctaa acaaaaaaac
    21421 gttttcttta ttatacatta ttcgagtttt tatttgtact gctgcgctgt gggaagcaaa
    21481 cgagttattt ttcacacaaa aaaaccgaga acttgaataa acaagtttct cggtttcatc
    21541 attttccttt tatcggctta gtaccagccg tttgctaacc agaatgtttt agcagcatcc
    21601 catgaaccat aacgagatgt tacatattgt tctgctacac gttcttgatt ggcagctgaa
    21661 tagtcgccgt ttaagtatga agcatctaat tgataacggc cgatgtaacg tccgttagta
    21721 gctgtgtatg aaccgcctga ttctttttgt gcgatccatt cttttgcaga gcttgaagtt
    21781 ggcgctgttg cagcagctgg ggcagcagga gctacttcaa ctgtttcaac aggtgcaacg
    21841 tatgtatgtt cttgcgttgc tgtttcagtt gtagatgctg tattttctgt tgttttgttt
    21901 tctgttgttg ctgaagaatc atttgaagtt gggattgtta attgttcacc aacataaatg
    21961 cggttgatgt ttgcgatcga atttttttca gcaattgcat cgatcattga gttgtctcct
    22021 gcaaattttt gtgagatctt agataatgaa tcacctgatt ttactgtata aacttcatca
    22081 gcgtgagcat ttgtagttcc tacaaacatt gcagtagcac cagcagcaaa agttgtacct
    22141 aaaacaattt tcttaaatga attcatatta aactcctttt tttctccgtt ttttattctt
    22201 tcgacgctat taactttatc atttagaaat atttcatagg taaaagaaac atgacattta
    22261 atgacaaaat aacgaattat ttaatatttg taacatttaa acaaatattt ttttgataca
    22321 aaaccataaa agccttgttg tataagcaaa gtaatcgttt ttctatgttt ttttattttt
    22381 gaatgaaa
//
LOCUS       DAZUMM010000046        21940 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000046 DAZUMM010000000
VERSION     DAZUMM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.046"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..978)
                     /locus_tag="ACG8E6_001641"
     CDS             complement(7..978)
                     /locus_tag="ACG8E6_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFS5062056.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            1684..3063
                     /gene="brnQ"
                     /locus_tag="ACG8E6_001642"
     CDS             1684..3063
                     /gene="brnQ"
                     /locus_tag="ACG8E6_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS5062057.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(3219..3665)
                     /locus_tag="ACG8E6_001643"
     CDS             complement(3219..3665)
                     /locus_tag="ACG8E6_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFS5062058.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(3692..3868)
                     /gene="rpsU"
                     /locus_tag="ACG8E6_001644"
     CDS             complement(3692..3868)
                     /gene="rpsU"
                     /locus_tag="ACG8E6_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFS5062059.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(4030..4458)
                     /locus_tag="ACG8E6_001645"
     CDS             complement(4030..4458)
                     /locus_tag="ACG8E6_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS5062060.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            4550..5410
                     /locus_tag="ACG8E6_001646"
     CDS             4550..5410
                     /locus_tag="ACG8E6_001646"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFS5062061.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(5504..5854)
                     /locus_tag="ACG8E6_001647"
     CDS             complement(5504..5854)
                     /locus_tag="ACG8E6_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS5062062.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(5870..7597)
                     /locus_tag="ACG8E6_001648"
     CDS             complement(5870..7597)
                     /locus_tag="ACG8E6_001648"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFS5062063.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLIDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(7811..8737)
                     /gene="trxB"
                     /locus_tag="ACG8E6_001649"
     CDS             complement(7811..8737)
                     /gene="trxB"
                     /locus_tag="ACG8E6_001649"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFS5062064.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(8882..10315)
                     /locus_tag="ACG8E6_001650"
     CDS             complement(8882..10315)
                     /locus_tag="ACG8E6_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS5062065.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDKDHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     NEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDTVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(10315..11718)
                     /locus_tag="ACG8E6_001651"
     CDS             complement(10315..11718)
                     /locus_tag="ACG8E6_001651"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS5062066.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMATMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     DLIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            11948..12850
                     /locus_tag="ACG8E6_001652"
     CDS             11948..12850
                     /locus_tag="ACG8E6_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5062067.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(12891..14777)
                     /locus_tag="ACG8E6_001653"
     CDS             complement(12891..14777)
                     /locus_tag="ACG8E6_001653"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5062068.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLKAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14874..15725)
                     /gene="licT"
                     /locus_tag="ACG8E6_001654"
     CDS             complement(14874..15725)
                     /gene="licT"
                     /locus_tag="ACG8E6_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS5062069.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQNNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(16060..17844)
                     /locus_tag="ACG8E6_001655"
     CDS             complement(16060..17844)
                     /locus_tag="ACG8E6_001655"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5062070.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(17844..19595)
                     /locus_tag="ACG8E6_001656"
     CDS             complement(17844..19595)
                     /locus_tag="ACG8E6_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFS5062071.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(19737..19961)
                     /locus_tag="ACG8E6_001657"
     CDS             complement(19737..19961)
                     /locus_tag="ACG8E6_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFS5062072.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            20212..21798
                     /locus_tag="ACG8E6_001658"
     CDS             20212..21798
                     /locus_tag="ACG8E6_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFS5062073.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         6201 a         4734 c         3899 g         7106 t
ORIGIN      
        1 caaagcttat tcttgatgaa gatccgcctt ttcataagct ttgatgattt cggcaactac
       61 agggtgccga acaacgtcac ttgcttcaaa attaacaaaa tcgatttttt tgatttgctt
      121 taatgttcgt tctgcatgaa caagaccact tgtcgtccca cgaggcaagt ctatctgact
      181 tgtatcccca ttgacaatca tttttgattg ataaccgaga cgggtcaaga acattttcat
      241 ctgagcgact gtcgtgtttt gtgcttcgtc caagataacg aatgcatcat ccaatgtccg
      301 cccccgcatg taagctaaag gtgcgatttc gatcacgcct cgttccatca atcggttcgt
      361 atgatccaat ccaaaaattt ggtataaagc atcataaaca ggccgcagat aaggatccac
      421 tttttctttc agatccccgg gtagaaagcc taggttttcc cctgcctcca cagcgggccg
      481 cgtcaaaatg attttttgca cttcgccttt ttttagggca gcaattgcta atactacggc
      541 aagaaacgtt ttccctgttc cggctggccc tacaccaaat acgatgtcat gtttggctac
      601 cgattcaacg tatttctttt gaccgctgtt ttttacccgt ataggttttc cattacggtc
      661 tttgacgatc tcttcctcat acatctcaac aaaataatct aatgtacctt tttgccccat
      721 cttcaatgcc gtcacaacat ctggcgtact tacgtggatt cctcgtttga tcaattcctg
      781 taatgtccga atgactgagg cagccaagga aatctgggat tgctcaccaa tgatctgaat
      841 gatttctcca cgtgtatgga tggttgtttc cgttgtttcc tcgatcagtt ttatatgctt
      901 gtcgtgtgca cctaaaagca tgctgacatc atcagcagct gtcaatctaa tctctaaaga
      961 actagactct tccgtcaaaa atagacatcc cctttttttc gattcattct ttcctatttt
     1021 accatactca gaagaaacta tatagcaaat aagaaaaggt tgtaaaagag tcgtttaacc
     1081 atacgtacca agaaacgtta aaaaatagcc gtttccattc gctaaaggtc tcgttttgat
     1141 aaaaagaagc tgagaatgta agttatatga aagtggagaa caaaatattc cgtttcattc
     1201 agcaggtatt ctctaggtat ttttcttgtt tcaaaaaaag aaaacaaaat agttaaaata
     1261 ttcttgcatt tcaaaaaaaa caggtgtatt attacgtcaa caaataaaca tacagcaaag
     1321 cgtcgaggaa aacagtaggc tattcaacga tttattccca gtgagtcatg taagtgagaa
     1381 atgacaataa atcgaataga cgaacatcat ttccgagtca atgtcgaaaa cgtagatcat
     1441 tgtggctgcc tccattatca ggctagcgga taactttatc aaaaagcgtc cgtgaatgaa
     1501 gaagcttttt taagcgaaaa aaggtggtac cgttctagaa tctagagccc ttttcccaag
     1561 tggaaaaggg ctttttttat attttaaggt ggtggttttc atggaatctg atattagtgg
     1621 cattactaat tgctgcaacg tttatatcgt caatcaaaat gataattatt ggaggaaaaa
     1681 tttatggaag aaacaaaaac ttattcatgg aaacaactat tgatggtcag ttcacttatc
     1741 ttcgggatgt tcttcgggtc aggtaatcta atctttcctg tccacttagg tcaaatggct
     1801 ggcgcaaact gggtcagtgc tggcgtagga tttgccatct cgggcgctct tttcccatta
     1861 ctagctatct tagctgtagt agtgacaaaa agtgatggtc tgtatgactt agctaaacct
     1921 gtcggaaaat ggtatgctgc cttgttctta atccttgttc atttgacgat cggaccattt
     1981 ttcggtacac cacggacagc tgctactgct tttgaaatgg ctgctaaacc atttttgcct
     2041 gaaaaatata attcattagg tatgttttta ttctctgctc ttttcttttt acttgcttat
     2101 ttccttactg tccatcccac acggttggtc aaatacgtcg gaaagtatct aaactccttg
     2161 tttcttgcat tgttagcaat catttttgtc gtttctttat tccacccaat gggaaatcta
     2221 aatcaatctg ctgcagatac ttaccaaaca aacgctttaa caaatggtgt cctagaaggt
     2281 tataacacgg ttgatgcagt agccttgctt gccttaagtg tcacttttgt ccatgcagtc
     2341 cgtgaccttg gtttcaaaga taaaaaagtc acagaattaa cagcaaaagc tggtagcctg
     2401 agcgttgcgc tagaagtcat gatctatttt ggtcttgttc ttctcggtgc gatgagtttg
     2461 aataagatgt ctttatcaga aaatggcgga actgcacttt ctcagatcac taatttttat
     2521 ttaggtaact tcggcgctag cttcttagga gtgatggtca cattaggtgt cttcactacc
     2581 gctatgggac tcgttgtttc ttttgcgcaa gatttccata aactgttccc taaagtcagt
     2641 tatatgacat ggctgcggtt aacgactttc gtctcttttg tcgttgcaaa tgctggttta
     2701 gacaatatca tccaatggtc tttaccagta ttgatgcttt tgtatccatt gtcgcttgcg
     2761 ttgatcttgc tttccttaac agcaaaattt ttccaaaaga caccatttgt ctatcaagta
     2821 acgatgttgt ttgctgctgt tcctgccgta ttggatatgc tggcaaattc gcctgcttta
     2881 gtgagtcaac aaagagttgt agcaagcatg ctcgaatttt atcatcatca cgttcctttt
     2941 gctgcattag gacttggctg gatggtccca acgttacttg gttacgctgg cagcctactg
     3001 ttttattacg cctatcgcct atcagggtat aaacaagaag caaatgaact accagaagaa
     3061 taaccaaatc aaacacaatt taacataaga aatattaaac aggaaaaaac cagctagatg
     3121 aaagaatcct attctttcgt ctagctattt ttttgttctc ttttttacgc gcacaaaaaa
     3181 actgcggaca atgattgtcc gcagtttaaa tgataaaatt aagattgtaa tagttctttc
     3241 acaatggcat ttacttggtt tccatctgct tttcctttga ctttaggcat gatcgcaccc
     3301 atgactttgc caaattcttt cattgatgaa gcaccagttt gtgtgattgc ttcttgaaca
     3361 agttgtcgaa tttcttcgtc ggaaagttgt tccggcatgt atttttcgac tatcacaatt
     3421 tcacttttta ctttttcagc taaatcgtca cgtcctgctt cttcaaactc atgcaaagaa
     3481 tcacgtcgct gtttcatttc gcgagacaac accgttaatt cttcttcttc tgtcaagtcg
     3541 tgaccttctt taatctgctc gttttgtata gatgacttga tcattcggat gacttgtaaa
     3601 gattccttat ctttcgcttt catcgctgtt ttcatgtcat cgtttaatct acttagtagt
     3661 gacacactac caactccaat tcatcacaga actagaattt tttgcgtttt ctagctgctt
     3721 cagatttttt cttgcgtctt acacttggtt tttcgtagaa ttcacgttta cgagattctt
     3781 gtaaagtacc cgcttttgac acggaacgtt tgaagcgacg aagagcatcg tcaagtgatt
     3841 cgtttttgcg aacgactgtt tttgacatgt taattccctc cctccgagct taaaaagtaa
     3901 ccgtaattgt tattgaaata tatctcaaaa aacaagactg tcctgtaaat attatatcta
     3961 atgctgtttt gaccgtcaag gaaatcttgc ttttttttaa aattatcaaa tgttatttta
     4021 tttaaatcct tattgacaat tttcacatct tccgaaaatc tcgaatctat gcccttcgat
     4081 cgtacatcca ccgagctgtt cttcgaaata attcattgga cacatatgga tttctttcgt
     4141 tgttccgcac accgtacaga taaagtgatg gtgatggccg atttcccctt gacagcaatg
     4201 aaaacgaaac ttcatctctc catttaaatc ggtttcttcc agtaaatcga tttctgcaaa
     4261 atcatggaga tttcgataaa tcgtatcgta acttactcct ttgaattcct gattcatata
     4321 gtcaaaaact tctttagcgg atacgtagcg attgcgcttc actaaataag ttaacaatgc
     4381 ttctcttttt ttagtatatt taaaaccgtt atttttcaat gtttctagtg attgttcaat
     4441 caatgtctgt tttttcatga cttattcctc caaatcaaaa tgattacgaa gtacacattc
     4501 tagagtatgg tataataaac ataaaaaaaa tgcaagtgag gttttatcca tgtcttccga
     4561 ttctacagaa atcgttactt tttttgtcat ctctgactct gctggagaaa ctgcgacaaa
     4621 attagcgcaa gccactatgg cccaatatcc taccgttgag ttcaatttat tccgcagaac
     4681 atttgtgaca gatgagaaaa cattgatgca ggcactgcaa gatgcactaa aagaaaaagc
     4741 ccttgtcctg cacacattga tcaatcaaga attgatcgag atgacgcgtt ctttttgtga
     4801 agaacatcaa ttatttagtt ttgatgtgat gactccgccg atatcagaaa tcgagcgctt
     4861 aactggtatc aagcccattc ggcaacctgg cgcccttcat ttattgaatg agaactattt
     4921 caaacggatc aaagcaatgg aatttgctgt aaaatacgat gacgggaaag atcctcgtgg
     4981 gtttctagaa gcagatgtcg ttttgctagg cgtatccaga acttccaaaa cgcctttgag
     5041 cttgttttta gctaacaaaa atttaaaggt tgccaatcta cctttgatcc cacaagcaca
     5101 tattcccaaa cagctttggg agatcgatcc taaaaaaatc gtgggactga cgaataaccc
     5161 agatatttta aacaatatcc gtaaagaacg gatgcgctct tacggattaa atcctgatac
     5221 tgcttattcc gatatcgaaa aaatacgagc tgaactcgat tttgcgaatg atctttatga
     5281 aaaattgggc tgcgtagtaa tcaatgtcgc ttctttatct atcgaagaaa cagcttcact
     5341 tattttgaat gctttagacc tagaagatca tagttattat ggaactgaaa ccaccgaaga
     5401 aacagactaa cagtttttct tgtctttttc ttaaaaaaac tggactgtgt cagcgaagaa
     5461 gattcgttga tacagtccag tttgtctcta tttgcaccta tcgttattga tgttccacat
     5521 gtttgagcgt ctggttcaaa atatccagta cgtgagaatc atcaagacta tacaaaatcg
     5581 cttttccctc acgatgggac ttgacgacat ggttttctct taataaacgc agttgatgcg
     5641 atacagcaga ttgttccatt tctactgctt gggtgattgc tgtcacattt tgttccccgt
     5701 ctttcagata cagcagaatt ttgagacgag tggggtcact caacattttg aatagttggc
     5761 tgactttctc tacttttgtt ttttgcgtgt ctggcaatcc attcacctcc aacttgaaaa
     5821 aagactgact tctaaagttt accacttttt ccatcagtct caagtttttt tattcttctg
     5881 tcattttttt gataatcgct tcataagcgg ctactttttc agctgcgtct tctgaagagt
     5941 ttccttttac accgatataa aatttgattt tcggctcagt tcctgaagga cggatagcga
     6001 tccagccatt ttcttccagc gtatacttca atacgtcaga tggcggtgtc gttagttttt
     6061 ctacgtttcc ttcacggtct gttctcgtca actctttgaa gtcttctgtt tggatgactt
     6121 ttgctccggc aaattctgtc ggtgcttctt cacggaattt ttccatcaaa gcagagattt
     6181 ttgcagaacc ttcttgtccg ctcattgtca cagaaatcgt tttttctgcg tagtagccat
     6241 atgtttcaaa aatcgtttgc aagccgtcat acaacgtttt gccttgtttt ttatagtaag
     6301 ctgctacttc tgctaataag agcaatgctt gaatcgcgtc tttgtcgcga acgaaaggtt
     6361 taactaaata accgtaactt tcttcgaatc cgaacataaa agtatgggaa tgatcttctt
     6421 caaattgttg aattttttca gcgatgaatt tgaatcctgt caatacattg aacatggtag
     6481 ccccatagtg ttccgcaatt gctgtaggca attcgctaga aacgattgat ttcaataccg
     6541 ctgcattttc aggtaaagtg cctcgcgatt ggtgtgcttc caaaatatat tgagcaaaca
     6601 gggctccgat ttggttccct gtcaatactt cataggagcc atttggcaaa cgaacggctg
     6661 cgcctaaacg gtctgcatca ggatctgttg caatcagtaa gtcagcgcct tctttctcgc
     6721 caagtcgcat cgcatactcg aaagcagagt gttcttctgg attcggtgat ttgactgtag
     6781 tgaaatccgg gtctgcgaca gcttgttctg gaactagtac gaattgttcg aatccagctt
     6841 gtttcaacgc acgttcgccc aacatttttc ctgttccgtg aagtggtgtg tacacaagtt
     6901 tcaaatcttt acccattgtt tccaccaatt catgatcaat cgttactgct ttgatttctt
     6961 ctagataagc ggcatccact tcttctccga tgatcgtgat caatccgcta tgttcagctt
     7021 cgtcgtcaga taatacttca acttttaacg gattgctgac ttcacggaca tatttcgtca
     7081 atgcatctgc gtctgcagga ggcatttgtc ccccatcttc tccgtagact ttatatccgt
     7141 tgtaagctgc tggattgtgg gatgcagtta ccatgattcc cgcaaacgtc ttcaaatgac
     7201 ggactgcaaa agaaagctct ggtgttggac gtaaactttc gaatacataa gaagggatat
     7261 tgtgatgtgc taatgttttg gctgcttcca tggcaaattc tggtgattga tggcgtgagt
     7321 cataagcgat agcgacacct cggcgttttg tttcttcacc ttgtgtatcc atgaaacgtg
     7381 caagtccttc tgttgcttgt cttaccgtat agatgttcat tcgattgatc ccaggtccta
     7441 agattccccg catgcctgca gtaccgaatt ccaacggtgc ataaaaagca tcttcaagtt
     7501 tttcaggatc ttcacccaga tcaattaatt gtttttttaa gttttcttct aaattttctt
     7561 catgtaccca ttgttcatac acttgttccc aagacatgaa aataacacct ctttctgaaa
     7621 tatggttctt aataagtata gcattcactt attagatatg aaagtacgag gtctaaaaaa
     7681 cgcttaaatc aagaaaggat tcttttatca agcacggttg caagtattcg cataaatcgg
     7741 attcagtaga tttcacgcga aaaaaaagag taaaagaaca attgaaaccg ttcttccact
     7801 ctttttaaac ttatgcagtt gtttcttcta aagattccaa gtatttatac acttgttgtc
     7861 cagcgatccc accatcacca acagcagtag tgatttgtcg caaatctttt tcacgaacat
     7921 ctccgatggc aaaaacgcct ggtatattcg tcttcatttc gttgtctgtt ggaatccagc
     7981 cagcttcgtt cgtcaaccct gcttctctaa atggtgcagt taatggatcc agtccaacat
     8041 agatgaatac tccgtctgct gccaaatcaa actcattgcc ggttttgaca ttacgtgctt
     8101 tcacacctgt cactaccatt tcattcccaa cgatttcgtc aacgactgta tcccacataa
     8161 aggaaatttt ttcattcgca aatgcacggt cttggatgat cttttgtgca cgtaattcat
     8221 ctctacggtg gacgatgact acttcagaag caaattgtgt caaatagatg gcttcttcta
     8281 ctgctgagtc tcctcctccg atcactacta gacgtttgtt acggaaaaac gcaccatcac
     8341 aaactgcaca gtaagaaacg ccacgtccag caaattcttc ctcgccaggt acgcctaatt
     8401 tacggtggat acagcctgtt gcgataatga ctgtttttcc ttcgtaagtt ttatcttcac
     8461 aaataacttc tttgtggctg ccgtgatcct tgatatcttg cacgattcca taagcatttt
     8521 ctgtcccaaa tcttgaaaca ccttcataca tcttataagc tagatcagga cccatgattg
     8581 aatcgaaacc aggatagttt tcgatttctg ccgtattgtt catttggccg ccaggggcgc
     8641 cttgttcgat catcaatacg gataagtttg aacgtgaagc atataaagca gcagtcatcc
     8701 cagcaggacc tgcgccaata acgattacat catacatatg tagattctcc tttacttaat
     8761 caagtaagca tactttacag ccaatgcgaa aaaaagtcta ttgttctgct tgcgtttctc
     8821 gaatagcttt attatactcg aaaaatccgc tgaaaaacaa tagactttta caaatcattg
     8881 atcaatcatt cgaaagattt tctccatttg aatcaattac ttttttatac cagttgaatg
     8941 atttcttctt cgaacgggct aatgtgccat ttccttcgtt gtcacgatcg acataaatga
     9001 atccgtacct tttcttcatc tcacctgtac cagctgatac taaatcaata catccccaag
     9061 tcgtatagcc taatagatcg actccatcta attctactgt atctttcatt gcctggatat
     9121 gtgctgccag atagtcgatt cgatagtcat cttccacata accattctca tcgggcgtat
     9181 cgactgcacc taagccgttt tctacaataa atagtggttt ttgataacga tcataaatat
     9241 cattcatcgt tattctcaag cctaatggat caatttgcca accccattca cttgcttcca
     9301 gatatggatt tttgacagaa gcaaagatat ttcctgcagt ctgttccaag agttcgttgt
     9361 cagtagtcga aactcgagaa gaataataag aaaaggagat aaaatcaaca gtatgttctt
     9421 tcagcaatgc ggcatcccca ggttccattt tgatctgaat gccatttcgt tccatttctt
     9481 tcaatgcata agcgggatat tctcctcgtg actggacatc aatgaagaaa taattttctc
     9541 gatctgattt tctagaagcc caaacatctt caggattgca agtgtatgga taatatgctc
     9601 ctgctgccag catacagcct actttgttct ccggatctac ttcatgagca atcttcgttg
     9661 cgatggcact tgcaaccaac tcatgatgtg ccgcttgata tttgatttgt tcttcatttt
     9721 ctccttcttc aaaatacagc cctgcgccca tgaatggtgc atgaaggatc atattgatct
     9781 cattgaatgt caaccaatat ttgacaagtc ctttatagcg attgaagatg actcggcaca
     9841 gattttcata aaaaccaacg agttctctag aacgccacgc accatattct ttgatcaaat
     9901 gcatcggaca atcaaaatgt gtaatcgtca caagcggctc gatcccgtac ttttgacatt
     9961 ctttaaagac atcctcataa aatttcaaac cagattcatt tggctcattt tcatcgccca
    10021 ttggaaaaat acgactccaa gcaatggaca tccgataagt cttgaacccc atttctgcaa
    10081 acaaagcaat atcttcctta taatgatgat acatatcgat cgcaattttt gctggataaa
    10141 aatgatcttt atcaaattca aacattttct tttttccagt aatgacagga aaacggtctt
    10201 ctccgattgg cacaacatct acgttcgcta acccacgtcc gccttcatca taaccaccct
    10261 cgcattgatt ggctgctgta gcaccgcccc ataaaaaatc ttttctgaat cccatcatac
    10321 ttcctccatt acatgtgtat tgcttgacaa ttctcttgcc agttcatctt ggacttcgat
    10381 tccttctgct tttaaccggc tgattgtttc cttgaattgc ggtaaatact gctgatgtgc
    10441 aataaacaac tcatccatga tccgttttgc tgtctttcct gatcgtataa gcggattgat
    10501 cgtaaacgct tgtaaagctg ttccataatc accagtcacg gctgcttcga tcgtcagcaa
    10561 ttccatattc ttcattactt gcaaccagcc tctttcagca gcaggaaggg aaccaaacgc
    10621 tacatttcgt gcgccttgtg cgccaacata ggcagtgact tctactacac aatctgccgg
    10681 cagatcagga acagctccat cattctttgt agacaccacg atctgtgttt ctttatttcc
    10741 atagatcgaa gcaatcgttt cacaggcagc atctgaataa taagcaccac ctcgtttttg
    10801 caattgttca ggtttatgat ccaattctgg atctttgtat aattcaaaca gctctgcttc
    10861 tgtttctttc acttgttgcg ctcttgtacc aatcgtttga tattcttcta aagcatgttc
    10921 caacatttct tcttcacggt agtaatagcg gtggtagcca caagggatca tgttcatttg
    10981 ttttaattgt tctttgaaaa acgggatgtc aaagatattt ttcggcaatc cattgtcttc
    11041 atcgtataaa cgatcaatca aatctgctgt gcggtcattt cctttattat ctgctacttt
    11101 gtgccaatgg aagtgattta gaccggcaaa cttgtagatc agctcttctt ttttcttgcc
    11161 aagcattgct ggttctgtca tcgtcgccat tactggcaca ttgcataatc cgatcacttt
    11221 atcccatttg ccatatctga taactgcttc tgtcaccatc ccgcttgggt tcgcgaaatt
    11281 gatcagccaa gcatttggac ataaacgctt catgtcttca acgattgcca aaaccactgg
    11341 aacggtacga aaagctttga atatcccgcc agcaccattt gtttcttgcc caatcatccc
    11401 ataataacta gggatccgct cgtctttgat tcgtgcatct aattgaccga cacgaaactg
    11461 agtcgtaaca aaatccgcat ctttcaatgc ttcttcccga tccaaagttg catagatctt
    11521 gacttcatac ggagaagcat cccacatacg ctgagacatc tgactgacga tctcaagctt
    11581 ttcttttcct tcttctacat ctacgagcca gatttctcgt atcggtaatt cctcatatct
    11641 tttgataaat ccttccatca actcgggggt gtaactgctg cctccaccaa tcgtagcgat
    11701 tttgattcct tttgccatgt tctcactcct ttgcttttta cacttttatt atcttttttg
    11761 taaacaaaac aaacaataga aattcgtttc tttccagtaa catttacaaa agttagtgtt
    11821 ttctactgga tttttgtaaa tatcttgtca aaaaatgact ttattaagct atataacata
    11881 aatccgttta caaatcattt tcaaaaaagt tataatttac gtaaaaaaac tagaagaagg
    11941 tatctacatg ttattacgtg aaaaaatcaa agaaatctcc ttttctcctt cagaaaatga
    12001 agtgattcag tttctcttaa gcgattctcc agcacttggc gaaatgacca tccaagagat
    12061 tgctgagcga tgttatgtcc acccgtccac actcatccgt gtagcaaaaa aaatgggttt
    12121 tgatggctgg gtcagttttc gtaatgcttt tttagctgaa acagcctatc ttgaagggaa
    12181 ttttcaagac gtagatgcga atcttccttt ttccaccaca gacggtatta tgacgatcgc
    12241 aaataaaatg gcttctttag aacagatgac gattcaagat acactttctc tcattcatca
    12301 tgaccaactg caaaaagcca aacaactaat gcttcaagca aaaaaaatcg tgattttcgc
    12361 cagtaactcg aatactttga tttctcaaga tttcatgctg aaaatgaagc gcatcaaaat
    12421 ggatgtcacg attgtcacca cttttggtga atcgttctat gaagcgtaca attgcacgag
    12481 tgatatgtgc gggattctga tttcttatac gggtgaaaac aatgtgatcc ttcgtaccgc
    12541 agaaatattg aaacaaaatc atgttccaat tttagcgttg accagtatcg gtgattcaac
    12601 gctatcctca atgagtacag ccgctttgcg acttacgaca cgcgaacgac tgtattcaaa
    12661 aattggtaat tttacgatca atacatcgat ttgttatctt ctagacgtgt tatatagttg
    12721 tgtttttgca gaaaattatc aacagaattt agtccatcta atttctgtag gacaaaaatt
    12781 tgataaacgc cctatcagct cagccatcat gaaagagcct ttcaattttg aaaataaaga
    12841 agtggactaa aaagaatagt taagtctatt ccattctttc acctaaccgt tcaggtcagt
    12901 gcggtgataa gtgcatcgtc tggcccgacc gtctcccctt gattttccaa aatatccaaa
    12961 taatccatgt aattggtgac gattacaggt gtttctaaac tgtatccttc cttttcgatt
    13021 ttctttttgt cgaagcggag caacagatcg ccttgtttca ctctttctcc ttgctgcacc
    13081 aagctttcaa aatatttccc ttccaactga acagtatcca atccgacatg aatcagcaat
    13141 tccagacctg tttcagaaac cattccgatt gcatgtttcg tggggaacag ggtcatgact
    13201 gttccgtcaa aaggggcacg tacttctcct ttttcagggt agatcaagat tcctcgacct
    13261 agtgttcctt gtgcaaaagc tgcatccttt gctgctttca gtggagcgat ttgtccttgt
    13321 atcggtgttt ttacaatttc tttacctgat cttttctgtc tgtcatctgt ttcacctgct
    13381 tcctccttcc aaaagaaaaa agtcagcgca aaggatacca ccatcgcaat ggcgatccca
    13441 atcaatacat taatcacatc tgttaaatca ccatctggtc taatatagtt gggaattgta
    13501 aagatgccta gtccaccttg attccaagaa gttaaattca ttaacccact atacgcgccc
    13561 cctaacgcac caccgacaca agtaaaaata aatatttttt tacgtggtaa tgtaaaccca
    13621 taaatagctg gctctgtaat ccccacaata ccagaaacaa tggctggtat tgcaattgct
    13681 ttgtcttttg gatttctaag cttaaagtag agtgcggtaa gtgctgcagt ttgtccaaaa
    13741 gacactactg caacaggtgt cataatattt tgccaaccat tcacagaaaa ttgcatcaat
    13801 cccattggta cgattgccca atggagacca aacatcacca gaatttgcca gaagaagcct
    13861 acgattgctc cataaataac tggcgaaagt gctaaaagtg tttcaaatcc agccattaaa
    13921 atatcagttg cgaatgtgaa tactggtcca acgagtaaaa aacctaaagg cattgtaata
    13981 agaacggtga agaatggaac gaagaaattc gcgatcatct caggtactaa ctttttaatt
    14041 ggtttctgaa tttggctagc taacaacaca ataaagataa tgggaatgac agaagaagcg
    14101 tagttacgcg caatccaggg aatgccgaat actgtttgat aaataggtgc ttcaaaaata
    14161 gtgccagaaa atatggtcgt tagtggtgct tctgctgcac cactcaaagc ttccagcgac
    14221 aagctaggat tcattaatgc tgcgccaagc aacatcccta cgaattcatt tactttaaat
    14281 ttacgggcag ctgttaaacc gataataaca ggcataaatt ggaatacaga atcaccgata
    14341 ttatcaaaca taacaaatgt gccagaaccg cggtcaaacg cgcctaagaa gacaaggaat
    14401 gctgtcagtc cacgaatcat cccgccagca gctaagatag caaggaatgg ttggaaacag
    14461 cccgatattc catcaataag catatcaaac ggtctcattt tttctgttga aacagcctca
    14521 tcaaaagtaa ggtttcctgc ttttataacc tcttcaaaca cgactggcac atgattcccg
    14581 atgacaactt gatactgccc tcccgctcgc attaccgtta caataccgtc catatttttc
    14641 aagatctcat catttgcttt ttcttcgttt tttaatttga aacgaagcct tgtgatacaa
    14701 tgtgtcaaac cattgatatt ttccttccca ccgacatttt ctacgatttt tctcgctaat
    14761 tcttggtatt ttcccatttc tattccctcc aatatattag tggaaggttt agccaactga
    14821 atgtcactac ccttatttca tacgttcttg ttaatttatc ctcctttttt tatttattta
    14881 caacgattct catgcactaa acgcgcagta tggatcgtta aatacatttt ttcgtccgtg
    14941 gacatctggt agttgaacgt ctcggctaaa aagtctgcta ttttctcaac gcaaccaaac
    15001 gtatccttat attttttttg aataacctga aacaattcct cagaaacttc tcccacttct
    15061 gatttttctc catctaggac gcgtgtcaga aaaaaacgta agtgtgtgac gaatcgaaaa
    15121 aaatggactg aatcttcatc aaatgtaatc ttatagtaat agcggacgat atttagaata
    15181 tcctgtattg ttatagtaaa caggtaggta ttgttttgtt ggctttgaat gccttgtgca
    15241 ttgactaaat gcaatgcgat aaatccggct tcatcttctg ataacttgat gtggtattta
    15301 tccgagattt tcttcactgt ttttttcccg atggcatact ctttaggaaa tagttgtttg
    15361 atatcataca gcaatacatt tggtacaaat attcccattt tgtgacgttc gatagccgta
    15421 tacaaatgat ccgtcaatgc aatatacaca gaatcattca actggacttg tagagcctct
    15481 tttgtatatt caatgatttc acaagaaatt tctaagtact caagaggaat cgcttgtgcc
    15541 aattcttgga actgttgtga aagcccgtca tttgtcaact gaaatttttt gtcgatgcat
    15601 gagtcaaaaa tcgggtcacc cactttcttt ttgaatgcca aaccttttcc catcacgatc
    15661 atttcctgat ttctttcatc cctggttatt acgacgttgt tattcagtac tttctcaatc
    15721 agcatgtttt gctcctttcc atgatatata gggctgtatt caaaacaaaa agaaaaaccc
    15781 aaaaagccgt ccatggaacc atgggagact catcaggttt agcttgcatc aggcaatcac
    15841 tatccaagtt gtatcgtttt ctttatatta tcgaaagcaa acgcttttgt caacgctttc
    15901 ttagtggcta ttaggcactt ttgactagct gtcgtcgtct tttttaaatc gggtgactac
    15961 gccactaacg ctcacagatg aagaatctgg gaagctctgt tttattttca ctctagcgct
    16021 taggacaaaa tgctactatg ctactgtcac aacctctttt tacacaccac tgctttgcag
    16081 tttgtacata tctgcataca aaccattttg cgctagaagt gtttcatgat tcccacgctc
    16141 tactatatga cctttgtcta aaactaaaat caattctgca tcccgaatcg tggacagccg
    16201 atgggcaatc gcaatcgttg tccgtccttg tcgcattttt gccaatcctt cttggatcaa
    16261 tccttcggtt tcagtatcga tatttgctgt tgcttcatcc aatactagta ttttaggatc
    16321 tgtcacgatt gttcgcgcaa aagtgatcag ctgtcgttgt ccactagaat agctggcacc
    16381 gtgttcgatc acttttgagt gatatccttt tggtaattca tcgatgaact tgtttgcttg
    16441 aacaaactca gctgcatggc gaatctgttc gtctgtgata gctggattca tcatccggat
    16501 atttcctgca atatctccat aaaacaagaa ggcatcttgc aagaccaatc ccattttctt
    16561 acgtaactct tccattggat attctcgaat gtcatgatca tcaatcaaga tttggccttc
    16621 ataaaattcg taaaaccgca tcaatacgtt gatgatcgag ctttttccgc ttccggtgtg
    16681 ccccactaat gcaacggtct cacccggatt agcgacaaaa gagatattgt tcaatacatt
    16741 gtgctctcca tcataagaaa agcttacatt gcgaaactcg atcttacctg aagtaatcgc
    16801 ttgatctgct ccaggatttt gttgcggtgc atattcttct gtatccatga ttttcaaaat
    16861 acgactaccg gcaaccatcc catctgtaaa ggtgctcaaa aagtccatca tttgagacat
    16921 tggattaaaa aatccctgga cataggtcgt aaatgcatag atcagtccaa cttctacggg
    16981 agaatgcaac gcatcgatcc caaacaaagt caacgccaac gcaatcgcca atgcatagag
    17041 aaaattgatg atcggactta acagaatcga attcgttttg atcatcgcat agcgcgtgtc
    17101 taaatactcg ttgttcactt gttcaaattc tttagctaaa cgagcttctt ggcgaaactg
    17161 ctggataata cgcatgccgg aaatcgattc attcaatttc gtgttcagct gacttaattt
    17221 ttcccgcata tttcggtaaa tacgagaact gaatttctgg taataccaga tgacgattcc
    17281 taaaatcggc aagaacacca aagtccacaa ggcaatttct ccattgattt gaaacatggc
    17341 aacgaaagaa gaaacgactg caaagatccc agtcaatacc atcaaaaaga cataccaaaa
    17401 ttcaaatagc gtttctgtgt cattcgtcac tcgagaaacg gtcgatcctg ccggtgtctg
    17461 gtcaaaataa cgcataccta gtgtatgcag cttttcaaat aatcttaccc gaacatactg
    17521 ataggtcttc aatgaagcca tggaatacaa ataccattgg aaaaaccaaa tgatactttt
    17581 gactatgact ccaaacaagt acacaccagc aaaaaacaaa ataacttgtg tcgtagctga
    17641 ctgtttagcc agatgatcat ccataaaagt ttggatgatc cttggcaata ggacattgat
    17701 cacagacagg gcaaacgcaa ataagatagc aacgatgaac gttcttctaa aaggtttggc
    17761 gaacttcatc agtcgtttga tgatcttctt ctgttcttta agtggaatcg attttgtcca
    17821 ttccgattga taattttcat ccatcaggcc tcgcctcctt ctatcttcgc ttctaattgc
    17881 tgtttttccc acattcgttc ataccagcca cctaatgcaa gcagctcgtg gtgggttcct
    17941 cgttcaatga ttttcccttg atcgacaaca atgatttctt ttgcatgcat cacgctgctt
    18001 aaacggtgag ctgcaatgat ggttgttttc tcttttctta tttcttttaa attatgcaag
    18061 atagcttctt ctgttttggc atctactgca gaaagtgcat catctaagat cagcaattca
    18121 ggatttttga tcaatgctcg agcaatcgaa atcctttgtt tctgtccgcc agataaagag
    18181 actccacgtt ctccaactaa ggtatcataa ccttttggca attccttgat ttcattgttg
    18241 ataaatgcta aagaagccgc atgctctact tcttcttctg aaagttttgg attagaaaaa
    18301 cggatattat ctcgaatggt catcgaaaac aggaagtgat cctgaggcac ataaccgatt
    18361 gatcctaata aagcattcaa tgaatattct ttgatcgaac gtccgccaaa ttggattttc
    18421 ccttgataat catcgtattc acgcatcagt aatttgatga ttgtcgtttt tcccgcacca
    18481 gttttcccaa caatacctaa tgtttctcct tcttggatcg caaatttgat ttgttctaat
    18541 gctggtgatt cttctttcgg atagttaaaa gaggaaatag ctacattcag ctcgcctttt
    18601 gccggggtct gcactgcacc cagttgttcc acgatatgcg tcttttcatg caaaagttca
    18661 ttaacgcgat cataagaagc atttcctctt tccaatacgt tgaacaatcg gccaatcgca
    18721 aacatcggcc aaacaagcat accgatataa ctaataaatg agaccatctg cccaatcgag
    18781 atcgtgcctt ccaagacata ctttccacct aaaataattg ttgcaacata agacagaccg
    18841 ataatcaagg taatgaatgg atcaaataag gagtctaaaa aattgactcg tttgttttta
    18901 ataattgcat cttcaatctt ttcttcaaaa tccgcgagat cttctttttc ttgtccaaat
    18961 gttttaatga ctttgatccc agtgatactt tcttgtgtct tatcattgat actagaaaaa
    19021 gcttcttgcg aatcacgaaa agcatcgtgg agttttgacc ccagcactcg tgaagtaacg
    19081 gctagcaaag gcagaggaat cagcgcaatc agtgttaagc gccaatcaat aaacaaaatc
    19141 atcgcaatga tcgttgttcc gcccgtaatc actgaatctg caaaggtcaa gattccagct
    19201 cctgctacat tctggattgc attcaaatca ttcgttgcat gagccatcaa atctccagtc
    19261 cgatattttt ggtaaaagag attgtccatt tctgtaaaat gtttgaatag ctgctgccga
    19321 agttctttct ccaaccgagc tgcacttccc caaatattcg tccgccagat ataacgaaaa
    19381 agatattgta agaaagctgc aaggatcaat agtaagatcc accaagaaat gatctttatt
    19441 gataaattct tatcggcgat ctcatcaatg atgatcccaa tcacttttgg aggaactaac
    19501 tgtaccaagg ccactgcgaa tagggagaaa atcccaatta aataatgttt cttttcctga
    19561 cgaaaaaacc aacctaattt tctaaaaatc gacattttac tcctcctttg tctttatcaa
    19621 gagcatagga ttgtctgtcc ttgtcttttt tgctgaatag tctgaatagt ctaacaaatt
    19681 acgaaataaa aaacggactg aaaaaatcag cccgcaaaaa gtctttgggt cagaacctat
    19741 tttttccctt ggttcttcat ttgctgcatc atctggcgaa ttttcttctc agatggtttt
    19801 tgccccattg acatcatcat tgatcgcaac atgtcctcat taacgggagg atttttttct
    19861 aaatagtctt tcatatattt acgcgctagg aaaaagccac ctactgcgcc gattagcaaa
    19921 gcaataatag caataagtac gacaatacct gttgatacca tataaatctc tcctttctat
    19981 ttccgaattg ctctcaatga tttattttac taaagtttat tgaaaaaaga aaggcgtatt
    20041 ctttagtttc atgcaaaaaa tagtggtttt tttagagaag aacaaaattt ctttcattaa
    20101 ccagcaagac acgccacgaa aaaaaattag gcataccgaa tctttttctt taattttttc
    20161 tttctgaggt gtagaatgta atcgtagata aataggaaag gtggtttttt cttgaataat
    20221 caatccaaca aacaatttca taagcatcga cttattgaac atacaaatgg tttatcttta
    20281 gctgaagtaa atggaacagt ggatgtacca aagggaaaaa gtttttttaa aacacttctt
    20341 gcttactctg gaccaggtgc attagtagca gttggttata tggatccagg taactggtcc
    20401 acttcgatca ctggtggaca aaatttccaa tatttattga tgtcagttat tttgatgtct
    20461 agtctaattg ctatgctttt acagtatatg gcagcaaaat taggaatcgt gacccaaatg
    20521 gatttggccc aagcaatccg agcacggaca agtaagacat tgggtgtcat tttatggatc
    20581 ttgactgaat tagccattat ggcaacagac atcgcagaag tcattggtgc agcaatcgcc
    20641 ctctacttac tttttgatat tccattggta attgccgtat ttatcactgt gttagatgtt
    20701 ttcgtccttt tattgctgac aaaagtcggg tttcgtaaaa tcgaagcttt ggttgtctgc
    20761 ttgattttcg tcattttatt cgttttcgtc tatcaagttg gcttgtctaa ccctgattgg
    20821 cacagtttat ttgctggctt tatccctaac agtcatacat tctcagactc acctgtcata
    20881 ggtggtgaaa ctccgctgac tggagcgtta gggattattg gtgcaacagt catgccgcat
    20941 aacttatatc ttcattcagc agtttctcaa actcggaaga tcgatcacca agacgaagaa
    21001 gatgtagcac aagcggtacg cttttctact tgggactcta atatccagtt gtcaatggct
    21061 tttttagtca atgcactgct attgatcatg ggtgtcgctg tattcaaaac cggttctgtc
    21121 caagatcctt ccttttttgg tctgtatgaa gctttatctg atccatcagc tatgagcaat
    21181 ggtatcttga ttgctgtagc aaaatctggt atcttgtcta ctttatttgc tgtagcactt
    21241 cttgcttctg gccagaactc tactattact ggtacgttga ccggtcaagt catcatggaa
    21301 ggattcatcc atatgcgtat cccgatttgg ctaagaagac tagtcacacg attgctttct
    21361 gtgattcctg tattgatttg tgttttgttt acaagtacaa aaggaacgat cgaagaacat
    21421 accgctttga ataacttgat gaatgaatcc caagtttttc tggcttttgc attgccattc
    21481 tcgatgattc ctttactgat gatgaccaat agccaagcgg agatggggag ccgattcaaa
    21541 aattcatttg ttgtaaaact tcttggctgg ttctctgtga ttagtctgac ttacttgaac
    21601 ttacgaggat taccaggtca aatcgaaagt ttcttcggcg agcaagccac ctcagctcaa
    21661 ctagcattgg ctgatcaaat cgcctatgta atcattgttc ttgtccttct cctgttggca
    21721 tggacgattg gtgatttata taaaggaaac aaaaaatatg aacagcgttt gcaagaaacg
    21781 atagaagaaa tcgaatagaa actgattctt ttacacaaag gaggttgtga cagaagtggg
    21841 cagcttcaag aaataagaag gaatttaaga aaattgcttt tcaaattttt gtgaaatttc
    21901 ggcttatttc cgaagaagtt acttctggaa caccgtgtat
//
LOCUS       DAZUMM010000047        21459 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000047 DAZUMM010000000
VERSION     DAZUMM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.047"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(230..841)
                     /locus_tag="ACG8E6_001659"
     CDS             complement(230..841)
                     /locus_tag="ACG8E6_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5062074.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(964..1089)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1171..1887)
                     /locus_tag="ACG8E6_001660"
     CDS             complement(1171..1887)
                     /locus_tag="ACG8E6_001660"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS5062075.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1893..2462)
                     /gene="scpB"
                     /locus_tag="ACG8E6_001661"
     CDS             complement(1893..2462)
                     /gene="scpB"
                     /locus_tag="ACG8E6_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFS5062076.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2446..3216)
                     /locus_tag="ACG8E6_001662"
     CDS             complement(2446..3216)
                     /locus_tag="ACG8E6_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFS5062077.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3213..3584)
                     /locus_tag="ACG8E6_001663"
     CDS             complement(3213..3584)
                     /locus_tag="ACG8E6_001663"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5062078.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3603..4490)
                     /gene="xerD"
                     /locus_tag="ACG8E6_001664"
     CDS             complement(3603..4490)
                     /gene="xerD"
                     /locus_tag="ACG8E6_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFS5062079.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4586..5059)
                     /locus_tag="ACG8E6_001665"
     CDS             complement(4586..5059)
                     /locus_tag="ACG8E6_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS5062080.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(5164..6027)
                     /locus_tag="ACG8E6_001666"
     CDS             complement(5164..6027)
                     /locus_tag="ACG8E6_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFS5062081.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYGFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(6223..8322)
                     /locus_tag="ACG8E6_001667"
     CDS             complement(6223..8322)
                     /locus_tag="ACG8E6_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFS5062082.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(8542..9651)
                     /gene="rpoD"
                     /locus_tag="ACG8E6_001668"
     CDS             complement(8542..9651)
                     /gene="rpoD"
                     /locus_tag="ACG8E6_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFS5062083.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(9675..11516)
                     /gene="dnaG"
                     /locus_tag="ACG8E6_001669"
     CDS             complement(9675..11516)
                     /gene="dnaG"
                     /locus_tag="ACG8E6_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFS5062084.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(11698..14013)
                     /locus_tag="ACG8E6_001670"
     CDS             complement(11698..14013)
                     /locus_tag="ACG8E6_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5062085.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(14198..15058)
                     /locus_tag="ACG8E6_001671"
     CDS             complement(14198..15058)
                     /locus_tag="ACG8E6_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFS5062086.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(15117..15749)
                     /locus_tag="ACG8E6_001672"
     CDS             complement(15117..15749)
                     /locus_tag="ACG8E6_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFS5062087.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            15916..16677
                     /locus_tag="ACG8E6_001673"
     CDS             15916..16677
                     /locus_tag="ACG8E6_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5062088.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            16703..19294
                     /gene="mprF"
                     /locus_tag="ACG8E6_001674"
     CDS             16703..19294
                     /gene="mprF"
                     /locus_tag="ACG8E6_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS5062089.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19482..20669)
                     /gene="tuf"
                     /locus_tag="ACG8E6_001675"
     CDS             complement(19482..20669)
                     /gene="tuf"
                     /locus_tag="ACG8E6_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS5062090.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            21037..21393
                     /locus_tag="ACG8E6_001676"
     CDS             21037..21393
                     /locus_tag="ACG8E6_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5062091.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         5894 a         4493 c         3749 g         7323 t
ORIGIN      
        1 caggttttta aagctttctt ctttttcatt gtagcgtttc ctttttttta taaacaaaca
       61 ttttcgtctt ttaaaaggag aagaatttca catttgcaaa gaattgattg atttttatgc
      121 ataaatcaaa aaaatactca aaacaaacaa ataaaacgtt tacattattg catgtcttgt
      181 atttttttcc aaaaaaaaag accaagaaat ttcttgatct ttttgatatt tatttgactg
      241 ctgtatgacg ttgttcgaac tgatgttgtt ttcgacttaa ccatggaaga agttgcgcat
      301 gcagcactaa aaagacagca ctcacgatga atcctttgat caagttgaaa ggaacgatcc
      361 caatcagtac atagtttgct aaagacatgc ccagcatctg tgttgcgttc agccctaaaa
      421 acattaagta aagaggcgta ataacgaaat aatttgcgat actcataaaa atagttagag
      481 cgatggttcc tgtaatgaca cctaaaaatt tgtttcttcc tttatgctgt ttctttacac
      541 taaagaaata ataaatcggg aatgtaaaga tcgttgttga taagaaacta gcagtgtctc
      601 ctaccaaatt atccggagaa aaacctgtag tcagcagatg taatactgaa cgcaagaacg
      661 ctgttgcaac tccggccgct ggaccgaata agaacatgct gatcaatacc gagatatcac
      721 taaaatcgat cttcaaaaaa ctaaaagccg gcatgatcgg aaaagcaaaa tactgtaaaa
      781 ctaatgcgat cgcagcaaac atcgctactg caaccatttt ctgtacacgg gtgttcttca
      841 tttgaaatcc tcctgttagg actcctcttc agcgaacttt ttgacgtttc tctccaaatg
      901 aaaaaactct attttccagg gaaaatagag ttagagttat ttgatcaagc gtcgtcaaat
      961 aagctctatc ttctttatcc agactatact gtcggtatcg gaatttcacc gattcagcta
     1021 tttttttgca aaataggtcg tggactataa ccaccggtcg ggaatttcac cctgcccctg
     1081 aagacgaacc ttatataatt tttgtttaca tattcattat acatatctat tcaaaaaacg
     1141 caacatctaa cacttataaa aagtaagtgt tcattttgag tggttcagca aactgctgac
     1201 ttcttttttg ttcagttcac ggtaatcccc aggacgtagg ccttgtaaag tcagatctcc
     1261 gtatttttca cgttttagtt tttgcacagg aagaccaact gcttgaagca tatttttcac
     1321 ttgatgatta cgtccttcgt ggatcgtcaa ttccacgata ctgtggtttg tcttaggatc
     1381 cactgataaa atttggaatt tagctggaga tgtttttcgt ccttcgatct ttattccttt
     1441 tgtcaaaggt gcaagcatcg attttgtagc gatccctttt acctttgcaa cgtaaacttt
     1501 atccacttca tgttttggat gtgtcaggcg ctgagcaaaa tctccatcat ttgtcagtag
     1561 caagatcccc gaagtatcgt aatccagtcg acctacagga tagatacgtt cttttaccat
     1621 cggcaaaaga tccgtaacaa cttttcttcc tttgtcatct gcaacagcag aaataactcc
     1681 tttaggtttg tacaataaat agtaaccgta ttcttcttga taaattggga cttcatccac
     1741 ttccacagtg tcatgtttcc caacttttat ccctaattcc cttacaactt gtccgtttac
     1801 ttttacacgt cctgcaagga tgaattcttc tgcttttcga cgagaagtga tgccagcgtg
     1861 ggcaattact ttctgcaatc tctccattac aattattctc ctttattcat tccatcaaag
     1921 aatagatctg gtgacacgtc ttcttcaagt gaaatttcca tttcttgtgt atcaggcagt
     1981 tcttctaatg atttcaaacc aaaataatct aagaagtagc cagttgttcc atataatatc
     2041 ggccgacctg gtccttctac tctacctttt tcttcaatca gtttatgggc agctagacgc
     2101 tgtaccgctc ctgaaacctg aactcctcga atctgctcga tttcagctct tgtgattggc
     2161 tgtttataag cgatgattgc taaggtttcg acggcggctt gagataatgt gtttgatgtc
     2221 ccttgcgcat atcgcttaag taagggagcc agctcttttt tagtcgttaa tacataatgg
     2281 ctttccgttt caaaaatatg caaagctgag tgcgagtttt gagtgtaaga ttcttttaat
     2341 tctttcacta aacttgtgac ttctgcttcg ctgagattta acagtccgct taattcttca
     2401 ttactgatcc cttcctcgcc aacaataaaa agaagggctt caagttcact taatttcgtc
     2461 atgttcggta tctcctactg ctggataaag cataattgat tcatagtttt ctttttgttg
     2521 cgcaatgatc tgcccttttt tcatcaattc tagcaaagcc ataaaagtag tgacaacttc
     2581 acttttagaa ggtgactcaa aaaaagaatc gaaagtacag ccatgcgcat ttctttttat
     2641 acgctcttcg ataaatacca ttcgttcttc gatcgttgtg tcatcgggcg tgatcgtttt
     2701 ttccactttt tgagaaaatt tcttacgttc taagattttc tgcatggcac ggatcaaatc
     2761 gatttttttg acttgtcctc gcttcaacgg cttttcagca tccgctaacc catcgatgtt
     2821 cataggttct tttgtgtaat aaagacttcg ttcctctgct ttttctgtca gtctttcagc
     2881 tgcatattta tatttacggt attctaaaag ctgagtgacc aattcttctc gaggatcttc
     2941 ttcccataat tcttcatcgt ctgtgacgaa ttcttggaca ggaagcaatg tcttgctttt
     3001 gattgccatc aatgtagcag ccatcactaa gtattctcct gcaacagcta gctcgagtgt
     3061 tttcattgta tggatataat tcatatattg ctccgtgatc gtagcgatcg gtatatcata
     3121 tatatcgatt tccatttgct gaatcagatg aagcagcaga tcgagaggac cctcgaatac
     3181 atctaattta atattgatct ccgtcaactc attcactctt tcttcttttt ctttgctagc
     3241 cattttccaa tgtaaggctc tgtgatttca gttgcacgga tggagacatc cgggtacttt
     3301 tcctgtaatt catctaacat ttgaaagccc atattctcac cgcgataaga cggattcaaa
     3361 gaaatgtcat ggatgatcag ctgatcgttc gttgcccagt aaataccaat aatcccttga
     3421 atgttggttg aaccttctgg cgtaaagaaa aaaagttcat aatcgtcttc agattcgtac
     3481 gtatcaatct ccttcaacaa agaagtttta cctgtcagtt tcttatggaa actcaatagt
     3541 cccatagcaa ttttacggtt ttctttccgg taatgtgtta acatcttctc accttcctat
     3601 gactaagccc ttggaaaatg ctgcttataa acatccgtca ttcttttctt cgtgatatgt
     3661 gtataaatct gagtcgtaga gatatccgcg tgacctaaca attcctgtac tgtccgcaaa
     3721 tcagctccat tttctagcag atgtgtggca aagctatgcc gcagcgtatg tggtgttact
     3781 tctttgtaaa tcccagcctc tcgtactaac tgcttcagat ttttccagat tccttgccga
     3841 gaaagacctt tcccatgatt attgacaaat acatgcattt cagatgcgtc ctttacaagt
     3901 aatggacgtg cttcgtctaa gtagcgttcc agccattgga tcgcataatc acctaaagga
     3961 atgatccgct ctttatctcc ttttccaact gtctgtacca gtccaagaga aagatgaaga
     4021 tctcccaatt tcaatccaat caattcactg acgcgcatcc cagtagcata catgacttcc
     4081 aagattgcac gatctcttat ccctaacgtt tttgtcgtat caggtgcttc aatcagcttt
     4141 tctacttctg tcagagtcaa tgtacttggt aatttctgtg ctttcttcgg cgtatcgata
     4201 tgttgcatcg gatcatgatc agtgatacgc tcttgccgta aaaactgatg gaatcttctg
     4261 agacttgaga tcatccgagt aacagttgct gtcgcttttt tttcttcatg caacttctcc
     4321 agaaaacgga tgactaaaaa gcgatcgaca tcctgccatg aagcaatttt ctgctcctgt
     4381 agaaaatgca agtactgttc caaatcacgt tcatagcttt gtcgtgtatt ggcggacaat
     4441 ccccgttcaa tattcaagta gtggagataa tcgttgattt gctctttcat aataatcccc
     4501 ctcatgcctc tctatcatat cagataccgc ttcattctga tagtcttttt ccataatctg
     4561 agcaaagaaa aagccgtcta gacactcaga cggactggtt tcttttttct ttttcatagc
     4621 attcctgaca gattccatgg aacgttagcc gatgatcttt tactaaaaaa tgataacgtt
     4681 cttctactac ttgctccact tcaagtaaaa gatcctcttc tacttcctcg atcgtgccgc
     4741 agttcaagca tagtaaatga tgatggaaat gtttggcgcc ttcttttctc aaatcataac
     4801 gggctaatcc atcattaaaa cttactttat cgacaatttt caattctgtc aagatttcaa
     4861 gggttcgata aacagtagca agtccaattt ccggactctt ttttttcact aaaaagaaga
     4921 tttcttctgc agataaatgc tctttctcat tttccaaaag cacaagcaga gtagcttctc
     4981 tttgcggtgt cagcttaaac cccgcttcat gtagctgctg ttttgttttt ttcatcgccg
     5041 aaatagcatt catgaccatt tactcaaccc caacaattta taataattac aaataaaaaa
     5101 agatataaca ctatgttaat taaaattatt ataagctagt taagaaaaac atgcaaggga
     5161 ttctcaatta gattttttga ctagttttgt cacaccgttt tcttgtgtaa tcaatccttg
     5221 tttcaaaaga ttacctaatg cgcgcttaaa tgctcctttg ctgataccaa aagctttcat
     5281 gatttcttct ggatccgatt tatctgtaaa gttgatttgc tgatctgcag aacgttctaa
     5341 aatggttaaa atcatttgtg catcatctga tatagcttca tatgctcttg gcttcaatga
     5401 aagattcagt acaccgtctg gtcggacacc aatcactcga ccttttatct gctcgcctag
     5461 acgaggttca ttatatcgtt cagaaggatg gataaaccca agatagaaat cttccgttaa
     5521 aacgtacgta cctgttaatt tcaagcggta tgctgttcct atcacatctt tgtttttcat
     5581 ttcttctgtt ccatatttgc ttaaagattt gaatattttt tcatctgcta aactgcccca
     5641 aatacggtct tttgcatcaa ctctaaggga aatcatcaag cgatcccctt ttttaggcca
     5701 tagctctgtc attgtcggca attcatcaag agatacgaca aagtctttat ctggaagacc
     5761 gatatcgaca aacgcaccta aatcttttcg tgtagcagta acgattccaa aaccgtaatg
     5821 accgatacgg cttttaggaa tttgtgtagt gatccgattt tcatgttttt gattttggta
     5881 accgaatcct tcaactgctt caccgatttt gtgttcacct tctgatttgc ttaagccaaa
     5941 ggtttgtccg tttttttgga caaagtagtt tttttcattt tcatcaataa taatacctgt
     6001 aaatatagtt gctaataaat cgttcatttt tttctccctc tatagtgaaa ggttttctta
     6061 atacatttct ttgctatcat aacataatgt tatctacttg aatagctgag ccttggtttg
     6121 gaaaggtgaa aaaaggagta tgacaacagt tgtgtcatac tccccttttt cgaataaacg
     6181 gtgttaaacg gcaggctcac agcctctttc atttgcctaa aattaattgc tcccatttcc
     6241 tccgaaccat ccagttttac agttgatggt tacgatgtaa aatggcaaag cactttctcc
     6301 aatgcctttc atcaatgcag attttgttga agtggcatat agattataat agccttcccc
     6361 attttcgatt ctggcttttt ctaatctgta acctccttca acgatatatc cacgttggat
     6421 acatgtagca atcactttgc tttggacacc tggtgcccac gtccatgctg gattagatgc
     6481 atcggcaata tcacttgctc tggcagattt gattggctga ttcgctgagt ttgaagagga
     6541 aggggtcgag gatgccgctt cttgctgtgc tgcttgtgcg accgcagctt gttgagcggc
     6601 ttgagctgca gctgctgctt gagcggcttg ttttgcttgt tcttcagctg cttttttcgc
     6661 ttcttctgct tttttctttt cctcttcttc ttttttctct ttatcagtga gcatcttatc
     6721 tacttgtttc aattgttcat tcaactgagc agatagatct gtatttttca ctttttctac
     6781 tgcttctttt gccgtcttgt attggtcgcg tgttgctgaa tccactactt ttccgtcttt
     6841 aaagactata gctgtttttt ctttagctgt ttgcaattca gcatattgat ttttagcttc
     6901 tgtttgtgct tgttgaatca attttccaaa atctgtgtta tccgtttcga ttgcttggat
     6961 ctctttatct gctttcaact tgatgtcatc tgctagatga tcatcgacga tggcaggtgc
     7021 tatgaaccac tcattcactt tttggatatc tcggatctta gattggatat cttggtaagt
     7081 tgtttctaac tctgaatatc ctttttcatt cttaagactg gataaatcat tttttagttt
     7141 tgataaatct tgatgaatca tatctgcacg aataaattga tggtgatcat ctgtataaaa
     7201 ggcagcaagt ttctcatgaa gttcttccat ttgttgctgt ctttttgctt ctgctgctac
     7261 ttcggcttct actttttgat gatggtcata ataagcccag cttcctccac cgagtagaag
     7321 tactgctgct aaaacgagat aaggtacttt ttttgatttt ttcttttgcg ctttatctgg
     7381 atctgttttt tctaagtttg attcttgatg tgttttttca gtatctggca cgattgttat
     7441 tgatttcgct tctgcttcaa acagcttagg ctggttctct tctttttcta tttttgcttc
     7501 aagatcagta ccctctttgc ttacagattg attttcagca tcggattcac tatttatttc
     7561 atcgggctct gttgactttt tgtctgtgtt ttcgagtatt tcttttttct ctgcttccgg
     7621 cagtttattc gttttttcct tttctaattg tttttcagta aatacttcag ttttttctgt
     7681 gatatccgtt tttacgtctt ttatttttac tgtttcttta cctgtggcgt tttctctatc
     7741 agagtctttt tcagtttctt gttccttttc tggctctact ttgttttcct gtgcaatttt
     7801 ttcactgatc attttgagca gctctttcgc gtgctcttct tttgtatctt cacgatgtgc
     7861 tttgatataa gcggatagta tcgaattttc ttcctcatta tcgacgattt ctttcagttc
     7921 agctactctt cttttttgtt ttgattcaag cgtttcttct tgattctttc ctgattcttc
     7981 cgaagtctca tgaacagttg ctttcttctg ttcatttttc tctatattag ttacttgatc
     8041 gtgagcaggt ggttcttcgt cttctggttc tgctttttcg aataattcca ttacagattc
     8101 tagaggtaaa tctttcaatt ccgaccattc gattggatcg tttaattctg tatcaggaaa
     8161 gactgcatct tcagttgatt tatcctgcat agctgcatct gtatttgttt cttcaattga
     8221 ttcttttatt tgctgatctt ctgcagttat ttgatgaccg cagtctggac agatagtatt
     8281 ctgctccatt tttttcgatc cgcaatttgg acattttttc atcacttagc gatcggctcc
     8341 tttactaggt ttacgctacc atttaatcta tcatattcta tacgtcacgc caatagctta
     8401 tttttaggat ttacttaaaa ataatgaatg aatttccttc ctatagaaaa aaaagcttga
     8461 gactgcgtag tctcaagctt tttttctttt aaaataaacc aaacagtttt actgatttct
     8521 cttctggtca atagtccacg cttattctaa gaaatctttt aattgttttg aacgagaagg
     8581 atggcgcaat tttcttaatg ctttagcctc gatctgacga atacgttccc gagtaacacc
     8641 aaacactttc cctacttctt ccaatgttct cgtccggcca tcatctaaac caaaacgtaa
     8701 gcgaaggaca ttctcttccc gatcagtcaa tgtatctagt acatcttcca attgttcttt
     8761 tagtaactca taagctgcgt gttctgctgg acttgttgct tcctggtctt ctatgaaatc
     8821 acctaagtga gagtcatctt cttcaccgat tggcgtttca agggaaacag gttcttgcgc
     8881 gattttcaag atttctcgga ctttttctgt tggaagatcc atttccgcac cgatttcttc
     8941 tggcgtaggt tctcttccaa ggtcttgcag caattgacgt tggatacgta tcaatttatt
     9001 aattgtttca accatatgaa ctggaatacg gatcgtacgt gcttggtcag cgattgctcg
     9061 agtgatggct tgacgaatcc accatgtagc ataggttgaa aacttaaatc ctttgcggta
     9121 gtcaaatttt tcaactgcct tcatcagtcc catgtttcct tcttggatca agtcaaggaa
     9181 ctgcatgcct cgtcctacat aacgtttagc gatacttact actaaacgaa ggtttgcttc
     9241 tgccagacgc tgtttcgctt cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc
     9301 ttcttccgca gttaaaaggg agacacggcc aatttctttc agatacatac gaaccgggtc
     9361 attgattttt acaccggttg gcgcagaaag atcttctgtt tttgcttgtt cagctttttt
     9421 ctcagcactc ttcaagctgt gaattgatgg atcaccattt tcatccacca cactgatacc
     9481 tgcgtcttca atcttttgga tcaatttgtc catttcatct gcattcaaag tgaatggtgt
     9541 agcaagctga ttggataaat catcatatac aacttgtcgt ttgggtttgt tttctttgat
     9601 gaaagctgct actgctgctt catacgtttt tttatctgtt ccatttgcca taaaagaagg
     9661 ccccccttga gtagctattt ggcttgtttt aactgtttcg ttaaattgat gatttcaatc
     9721 gtcaattcaa gctcaagctg tttgtttcca gttcgtctgg cttcttgctg catttgtttt
     9781 tttaatgtta tttcatcagc taagccagat ttgctgatca catctagcaa gtcttggatt
     9841 tctcgtggcg aactttcttc tgccaccggt aaatattcga tttccaccac caaccgcctt
     9901 aaattttcat cttgcaaaaa gtctaaaaac tctgcaaggt tgaattcata atgttgtgag
     9961 acatatgcat cgaataaaaa ataaacttct gcatatgcat catgaataaa ctgaaattct
    10021 cgttctttta aaatattacg gacagaaact tctttaaatg cacgatataa tagaatctgc
    10081 tctgcctttt ctacttgcgt caaaggtctc tttttccgtg cagtttcttg tctcgtaacc
    10141 tgagtgtgtt gactttcctg aggttcttga cgattttgct gatttgtcct tcgaagatcg
    10201 cgcagttgtt tttgtatcgt ctctctgccg atctgaaact ctgttgacag ttgagataga
    10261 tacatatctt gttcgattgg tgaaacaact cggctaagtt caactagtaa ttcttcgagg
    10321 tattcgatct tgtctttttc attatctaaa tttttccctt gttttaaata acgtgattta
    10381 aaagtaaaga ctgtttcgcg cccatgagcc actaattctt ggaaagatgc ttctccatac
    10441 ttacggagat attcatcagg atcaagcttt tctggaacga ctacgacaga aagtgctaat
    10501 cgactgtttt gccgaagcaa ttcaatccct cgattggttg cttcaaaacc tgcgttgtct
    10561 ccgtcatagc aaaagaccaa ttctttcgtc agcttttcca tttgttggat ctgttgagct
    10621 gttagacttg tccccataga agctaatcca gttttgatac cagcctgcca tgcagcaatc
    10681 acatccataa acccttcgaa caacaaaatt tctccgtttt tccggatatc gcttcttgct
    10741 ttatcaagat taaataaaac ttgtctttta ttgaacagtt ctgtttctgg actattcaag
    10801 tatttgggct gatctttttc ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg
    10861 atcgtctggc cttgagaatt acgaagtgga aacatcactc tttgataaaa acgatcagac
    10921 atacggccat catcatgcgt gacaaacaat ccagagcgtt ctaacaattc atttgaaatt
    10981 gattcattct tgaatacttg ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg
    11041 aattcttcaa ttaattctaa agacaaccca cgactttgca aatattctaa agctggctgt
    11101 ccagcttttg tatgcaatag catgtgatga tagatttccg ctgctttttc atgagcagca
    11161 atcagtttcc ccgtagcaga ttcaggattt tggacagcta tagtacgctg acgccattga
    11221 tcttctaacg gtatctgttc gaagtctgcc acttttacca ctgcttctgg aaaacttaga
    11281 ccttctagtt cttgaagaaa agtaaacacg tttccgcctt taccacaacc aaagcaatga
    11341 aaaatctgct tgtcttctgc aacagaaaaa gacggtgtac gttcttcatg aaaaggacac
    11401 agccccaagt aatttttacc agactttttc aactggacat attgtccaat cacctcaacg
    11461 atattcgttt gactgcgtac tgtttcgata acctcttgag gaatccgttg agccattttt
    11521 tcaccccctg ctctattgat atcttttggg tttaacagaa gatatttcag gagaataacc
    11581 ggtaaaaaat cgcactaact gaaatcagtg cgattttaaa ggacacaatt atacatgttt
    11641 gattgtatca tatttcattg ctttttcaag tataacacat ttctatctca ttaagcatca
    11701 atttgctact cttttcagta actgattttg aatatcagcc ttccagtaca atactcgaag
    11761 aacaatattt acgagcatga atacaaagaa gtatcctacg ctctctccca accctaagtt
    11821 tacttccaaa attccatgga acagcattgc agctacatat atccctacga tcgtcgttac
    11881 tgctaggaag atgcgcaatg ggttgaaggg ataacaagct tttacaactg ccatgcaact
    11941 gatcccaatc agcaagtaat acatcaaagt agtagtttca gcatttcccc atgcgaatgt
    12001 ttttccaagt agatacaccg caatgatatt cactactaca agcaaggcat ttggtaaagc
    12061 attaataagt gctgtaggca aataacggta agtgattttt cttttatctt gttcaaaaga
    12121 taaaaagaag gaaggatacc cttcaatcgc taaatcaatc agcgtgattt ggatagggat
    12181 aaacggaaat ccaattgctg taacggcaca tataatcgat aaaataaatg aataaatcgt
    12241 tttgataaaa aagatgcctg agactttcgt cacattgttg accactcggc gtccttcaaa
    12301 caggacttct ggcaatgtag taaaatcaga gtccaacaat accagattcg caatctgacg
    12361 tgttgctcca tcaccttctg ccatagcaat gctgcaatct gcttctctta acgccaaaac
    12421 atcattcaca ccgtcgcctg tcattgcaac cgttctgccg ctttctttca attcatttac
    12481 cagcaatttc ttttgctgcg gactcactcg tccaaaaacc gtatagcgat gagcagcttc
    12541 tcgcacttcc atttcttcag taatttgaga aagatcgaca tacgcatcat aattcggtaa
    12601 tcctgcacgt ctagcgatat tggagacagt aaaaggattg tctccagaaa tcactttcag
    12661 atccacacct tcctcatgaa gataagctag tgtttcttga gcgttttttc ggattggatc
    12721 atcgatttca aacagagcaa ccggctcaac aaatgctggt ttttcttcgt tcaattgctg
    12781 atttggagcg atccccaaca taagtacgcg atagccattc gcttgggctg tttttagact
    12841 gtccggaagc cgatcttctt ccaccaaacg ttcaggcgct cctacgtaca cgactcccag
    12901 ttctggaaat tccatcgcgc cccatttgcg atcagaagaa aatgcgactt tgtttgtcac
    12961 atcataccga ttagaaagct caaaataatc tctcatagcc cgcatagtga tgttgttatc
    13021 actgctttct gccagatacg tccccataat ttctgggatg gctttctcgt acagcggatg
    13081 tagtgcttct acccgctgga tactcatttt cccttcagta atggtccctg ttttatccaa
    13141 gcataatgta tcgacatggg ccagcgtttc aattgaatac atgtcttgaa caaggattcg
    13201 tttttttgcc agtttggtta cccctgtcgt taatgcaata cttattagca ataccaaacc
    13261 ctttggaagc atgcctaaca aagctgctgc agatgcaata acagaaattt tgatagaagc
    13321 atcgcggatg aacagtgctt ccaaaaatag tatgatcccc aaaggaataa tcacgaaact
    13381 agtgaatttg gatactttgc gaatcgaatc tacaagttca gaacgaatag ctttgtgagt
    13441 ctttgcctcg ctagtcaagc ggacagcata attatctgca cctactcgga taacttcagc
    13501 catgacagaa ccgcttgtca aaaagcttcc agaaagcaat tcagctccag tttctttgac
    13561 aatcaaatca gattcgcctg tcaaaagagc ttcatttgct tctgcccgtc cagatataac
    13621 ctgtaaatcg gctggtatct gttctcctgc tccgattttt accaaatctc ctaataccag
    13681 ctctttagta gaaatcgatt gttctttacc gtcccggatc actagtactt ctgattggga
    13741 aacaatcgat aatttccgta caagatttct tgcatgtatt tcttgataaa ttccaataac
    13801 gatattgacg acaataattg ctaaatacgc catgttggaa tacgcaccta caaataacag
    13861 acaaatccca ataccaaagt tcagaaaatt gaataacgtc aatatattat cgctgaatat
    13921 ttgttttgta ctttttgatg catcttcctg gtagtcattt tgctggccag aagccatttt
    13981 ctgaactacc tcttgctgtg taagtccatt cattttcttt ttctccattt tccgccaact
    14041 ttcaaaaata atcacgtgtt taagtctagc gtgttccaag attaaaaaca acttgttttt
    14101 ataaaattta tagaaaaaca cacgaatgat tttacaaaaa agatgccaac ctttttgttt
    14161 gaatcacccg tgtgctgttt ttattatcaa tctatcttta cttatattgt tcctccaaac
    14221 ggctcatcca ccaacctagt gctgtaccgg ctataaaaag aacaggacaa gctagtaccc
    14281 cagcaactcc caatcccgcg taatcggtag gaatgatcat aatcgttgaa gcaaaaacta
    14341 cacctaaaat gaaatggaat agcttgctat atgcctttga gaaaatgtaa tccatgattt
    14401 ttgacaatcc aagaacacaa agaatcccac caattccgat cggcacaatc acttcaagtc
    14461 gtacttcttt aaatccgtca gccatcgctt tatacaatcc catatacacc aaaaaattag
    14521 aagggctcaa tcctgggacg atcataccca aaccaatcaa tccgccggca atcagccatg
    14581 ttccaaaatt ctgatcgact tgcccgtcaa ataattgagc gccgtataat aaaaacaaat
    14641 aagccagaac aaaagtaaca accatgatga tcacatcaga agtatctctt ccttttttcc
    14701 ctgcttcttt ccataacgca ggaacagttc cagcgataca tccgacaaaa aaccataata
    14761 tcgttgtttc atacgcacct aataaaaaac tgactaaaaa agaaagtaga aagatgcctg
    14821 tcactccgcc tagaccaaca ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa
    14881 ggtgggctaa aaaagaaatc agccgctcat acatgccaaa gacagctgct aatgcaccac
    14941 cgctgactcc tggcaagata aatcctgaac cgataaacat tcccttcaca aaccgtaaca
    15001 tccagtcttt ggttgtgtac cccacttctt gattttccat tttttgcctt ttttccacta
    15061 tttgcgcact ccttctttca tttaatttta ttcatgattg aacatttcct gctattttac
    15121 ttaaagactt tttcataggt ctctattatt ttttcattca ttttgacaat atcaggataa
    15181 aacatgttac ctccattatg atagagatag ccgccattgt aaagcaagga ctggatccgc
    15241 caatatcggt attgtttccc ttcctcattt cctaaaagtg ggctcagcac atctttagaa
    15301 tacgcttctg ctaattctac tgtgttttct ccgccatgat tttttacata ggcaatataa
    15361 tctttcccaa aattatatgc ttggacagct gtccataaat cgcatccttg tttctttgct
    15421 tctactatca tttccgccaa atatttgaca ccttgatcaa tactgtcagc tggattatca
    15481 atgatctcag cttctccatg cagactttcc gagctctgca tgagatctgt cccttttcct
    15541 tttgattcgg tcaatattat cgccttgatc aaattctcgt agttttccat atgataattt
    15601 tttgcagctg ttgttatttc tatttgatag ctttccactc gttttaccga acgataagtt
    15661 cttcctccta aaaacaacac cgcaccaata accgtcagaa tgatcatgta aaaaagaata
    15721 cggataatag ataatcgttt tcgcttcatt tcatcaccta gttattttga catctttttt
    15781 tatcttacta tttctttatc ttcttggcta tttcttcctt gatttctgtg tacgccttaa
    15841 gaaaaaatga attttcctgt attttttgac atcttacgaa aatgatcgtt gtttttcagt
    15901 aaaaacaatt gcccaatgac tgatataatc gttatacttt acacagtagg tgattatgtg
    15961 aaaaaagaaa agaaatatgc aaaaatgcca gatgggagcg aaatttacta tgaaaagagc
    16021 ggtcaaggct tccctctctt tttactgcat ggcaatgacg gcagcggccg ctttttttca
    16081 gaacaggtcc ctgtactcga acgctactat accgtgtatc tagtagatag ccgaggacat
    16141 ggtcgttcga caaacgaagc atctatgttg aattttcagt taatggccga ggatctgaat
    16201 acgatcatgt tgctggaaaa aatcgatcag gcagattttc ttggatttag tgatggagca
    16261 aatctagcac tggtatttgc cagcagcttc cctaaaaaag ttcatcgatt gattttaaat
    16321 tctggaaaca cgcttgttaa aggcgttcgt ttttctgcca gagtcatcag taatattcat
    16381 tatgcatggg tatggttact ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt
    16441 atcaaacttt tgctgcatga catcgggctt acagaaaatg atttaaaaaa aatcaactcc
    16501 cccactttga tcatcgtggg aaaaaaagat gtaatcaagc taaaacattc tttgtatata
    16561 gctaaaacta taccaaaagc ttcctttgtg cttgtgaaag aacaaggaca tgaattagct
    16621 agaaaagatc ctgaacgctt caatcgagaa gttcttcaat ttctttctga aacataacta
    16681 ttaggaatag aaggtgattt atttgttaaa aaataccata caatggctga aaaaccacct
    16741 aggactgttc aaaacaatct ttttgatttc tgtcattgtc attattgtcg gtgaattgat
    16801 gtccatcggt aaaactctat ctatccagca attaggagaa acgtttgcca ctatacctgt
    16861 atggaaaagc ggactaatgc tattgattgg tcttatctct gtgttaccta tggttggtta
    16921 tgatattatt ttgaatcgga tgcttgaaca aaagcagaat cctcgttacc tgtttgagac
    16981 aagctggctg atcaatacga tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg
    17041 gcttcgctct gaactatatg gacaaaagaa agagagcaga aacgtgatcc aagcattatc
    17101 gaaagttttc ttgtttttga tggctggact ttctatttac agtctgcttt cttttttact
    17161 tgtagtcttc acgccagttg ctcctttttt gaagcaatat tggatatggt tgatcggcgg
    17221 cggtctatat tttcctgtcg tctttttatt tactactttc aaaaagaaag gattccttgg
    17281 tggtacctca cttaaaactc aaggacagct attgctagtt tcattgttgg aatggaccgg
    17341 tgtcctgttt agtttccttt ctgttggtta tttaatggaa atccggattg atctgtggca
    17401 aacaattcca ttattcattg ctgcttcagt gataggaatc gtgtctatga ttccaggaga
    17461 aattgggagt ttcgatgtaa tgatgattat cgggttgtcc gctattggtg tgccaagaga
    17521 aatagtcgtt gtgtggatct tgctctatcg cctgttctac tacatcattc cgtttttgat
    17581 tggaatcgtc tttttcttca aaaatatcgg ttccactttt gatcagcgtt attctggtat
    17641 ccctaaacaa ttagcaacag aaattgcgca taaaatcgtg gttgtcttat tatacttttc
    17701 tggaatcatg ctagtacttt ctgccactat cccgcaagct tttacagagt ttcgctggct
    17761 tcatagtttg aacccattga aatttcattt tattatccaa tttcctagta tactattggg
    17821 atttctttta atcattatgg gcagagggat cgcagcacgt gtgaaacgag catatttgcc
    17881 tacaatcttc ttgattgttc tagctttact ttatgtttta ttaagtgatt ttagtttcac
    17941 accggtggtt ttcttgtcta ttctgcttct gatcattctt gcttctaaaa acgaattatt
    18001 tcgggaacaa ttgatctatt catgggagtg gcgtacgatc gatggaatca ttattggtgc
    18061 actgtccttg ctttacatcg ttattggggt atacaatctg ccggatttcc ctcatcggcg
    18121 tcaccatttt atttcctttt tcttatttcc ttctgaaaag atttggtttt cgggattgtt
    18181 ggccatcatc gctgttagtt tcatgatcgt tctatttgtt catttccttc aaggagaaaa
    18241 gaaacagatt ggcgaagcct ttaatgaaga aaaggccctg aagatcctta caacttatgg
    18301 aggaaactcc gatagtcaat tgatcttctt aaaagataag cggatgtttg cctatgaaaa
    18361 agacggtgaa ccaactgttc ttctccaatt cgcttgcttc aataataaat gtattgtaat
    18421 gggtgatcct tctggtaaga aagaagattt tccagaagca atcgaagctt ttatcgaaga
    18481 aacagatcga ctatgctatt taccagtttt ctacgaaaca agcgaagaga ttgttatgat
    18541 tttgcatgaa ttcggctacg atttcattaa gatgggggaa gaagcttatg tagacctgaa
    18601 tagttttaca acttcaggga aaaaaatgaa gggcactcgt gctgtcttga atcggatcga
    18661 acgagaagga tttacatttg atgtcttaca accaccattt tctactgagc aaatgagtac
    18721 tttcaaaaat atctctgata attggttggg ttcacgaaag gaaaaagggt tttctttagg
    18781 ctttttctct gaagattatc tacaaagagc gcctattgca gttgtcaaaa atacccaaga
    18841 agaaattgtc gcttttgcaa cgatcatgcc tacttacacg aacaataaag taggaacgat
    18901 tgatctgatg cgatatgatt ctaaaaaagc accttctggc agtatggatt tcttgttctt
    18961 gcatttgttt gcctatatga aagaagaaca tatccaatgg ttcaatttag ggatggcccc
    19021 tctttcaaat gtcggaactt ccagaaagag ttttttacag gaaaggattg catacttggt
    19081 ttacgagttt ggttctcatt tttattcttt ccatggatta agagaatata aaagcaagta
    19141 cgctacaaac tgggtctcac gttatactct atattctaga gatagttgga ttgcatatgt
    19201 tatgattgct ttgctgatta ttgataatgc gccagttgag cagacacaag gtagaataag
    19261 tggtttgaaa aaatggatac gaagaaagta ttaaaaactg cataccgata gatgaacact
    19321 ctcatgatga ataaaagaga ctgaagtaaa acgaaagttc atgcttctac aatctcttct
    19381 tatacaattg aagaacaaag agcggtccaa acagaaatcc tgtttggacc gctcttttat
    19441 tttaaacatt tttcattacc aaaatattcg agtcgcgttt cttatgcttc gatctctgta
    19501 acgatacctg aacctactgt ccgtccgcct tcacgaattg agaatgtcgt tccgttttca
    19561 acagcgattg gatggatcaa gtcaacatca atcgtcacgt tgtcaccagg catgaccatt
    19621 tctgtatctt ctggcaatgt gattgttcct gtaacatcag ttgtgcggaa gtagaactgt
    19681 ggacgatagt tgttgaagaa tggagtatga cgtccacctt cttcttttgt caacacgtac
    19741 acttctgctt tgaattttgt atgtggagta atggaacctg gtttagcaag tacttgtcca
    19801 cgttcgatat cgtcacgttg gatcccccgt aacaacacgc ctacgttatc cccagcttcc
    19861 ccgtaatcta acgttttacg gaacatttct acacctgtta caactgcttt ttgtgtttca
    19921 ggtttgatcc ctacgatctc tacctcatca ccgacacgaa cagcaccacg atcaatacga
    19981 cctgaggcaa cagttcctcg acctgtaatt gagaagacgt cttccactgg caatagtaat
    20041 ggtttatctg tatcacgttc tggtgttggg atatattcat ctactgtatc catcaattcc
    20101 ataatagcag cttcagcatc tggatcgcct tgcaatgctt ttaatgcgga acctttgatc
    20161 acaggagtgt catcgcctgg gaaaccatat tcgctcaata actcgcgaac ttccatttct
    20221 accaaatcga tcaattcttc atcatcgact aaatcaactt tgttcaagaa aacaatcagg
    20281 tattttacac caacttggcg tgacaataaa atgtgttcac gtgtttgagg catcggaccg
    20341 tcagttgcag aaacaactaa gattgcacca tccatttgtg ctgcccctgt aatcatgttt
    20401 ttcacatagt ccgcgtgtcc tggtgcatca atatgcgcgt agtggcgttt ttctgtttca
    20461 tattctacgt gtgcggtatt gatcgtaatc ccacgttcac gttcttctgg agctgcatca
    20521 atactagcat agtcttgagg atttgctagg ccttttttgc ctaatactgt agtgatcgca
    20581 gcagttaaag tagttttccc atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt
    20641 ttacttctgt cataatgttc ttttgccata atttacaaac ctcctaaata atatagatca
    20701 ctgaaaaatt tcagctctca cctttttatt atagtctttt tttgactaaa tcaaaaagaa
    20761 tacgtccgac ttactttttg taagattcga actaagctta ttatacgtca atattggtca
    20821 aagtcaacta agagcactcg tctgtcttat acgcgaaaaa agtacctttt ataaataaaa
    20881 aaatgtgttt tttgagaaaa acttcaagat agtccttaac tttcttttga agcaataaga
    20941 acaaaatgag gaaaagttta aaattcagct gttgccaaca actttcaata gacgaaatct
    21001 tctatttcgt taataataag gaaagaggtg aagtacatga aaaaattaat tggcgtactt
    21061 gtcgttttag gcatatttct cggcggaagt tttgcagctt atcattattt ttacggcggc
    21121 gaagcttatt atacaaaaat cacgacgaat ggcgaaatgt cagctaccca agcagataac
    21181 ggagagaaat tcacgactta tacctacaag caagcggctt ataataaaga tggtgaaaaa
    21241 aaacaggtga cgcttcgcga agaaagagaa catccattaa agatgaatgc gtatcttaaa
    21301 ctcaaagtca atcctcgtaa aggtgtatta agctgggaag aaataaaggc ttctgaagtg
    21361 cctaaaaatg cggcagaaaa aatcgataat tagtaaatgg tcttaaaatt ttattagata
    21421 aaaagaggct gggacagaag cgtttaactc caagaaata
//
LOCUS       DAZUMM010000048        21032 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000048 DAZUMM010000000
VERSION     DAZUMM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.048"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            289..606
                     /locus_tag="ACG8E6_001677"
     CDS             289..606
                     /locus_tag="ACG8E6_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFS5062092.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(697..1062)
                     /locus_tag="ACG8E6_001678"
     CDS             complement(697..1062)
                     /locus_tag="ACG8E6_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062093.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1270..1752
                     /locus_tag="ACG8E6_001679"
     CDS             1270..1752
                     /locus_tag="ACG8E6_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062094.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1949..2839
                     /locus_tag="ACG8E6_001680"
     CDS             1949..2839
                     /locus_tag="ACG8E6_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFS5062095.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3165..5639
                     /locus_tag="ACG8E6_001681"
     CDS             3165..5639
                     /locus_tag="ACG8E6_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5062096.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5742..7478)
                     /locus_tag="ACG8E6_001682"
     CDS             complement(5742..7478)
                     /locus_tag="ACG8E6_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5062097.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7475..8179)
                     /locus_tag="ACG8E6_001683"
     CDS             complement(7475..8179)
                     /locus_tag="ACG8E6_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5062098.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8310..8813
                     /locus_tag="ACG8E6_001684"
     CDS             8310..8813
                     /locus_tag="ACG8E6_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFS5062099.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8773..9111
                     /locus_tag="ACG8E6_001685"
     CDS             8773..9111
                     /locus_tag="ACG8E6_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFS5062100.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9132..10550
                     /gene="ffh"
                     /locus_tag="ACG8E6_001686"
     CDS             9132..10550
                     /gene="ffh"
                     /locus_tag="ACG8E6_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFS5062101.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10662..11240
                     /locus_tag="ACG8E6_001687"
     CDS             10662..11240
                     /locus_tag="ACG8E6_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFS5062102.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11244..11765
                     /locus_tag="ACG8E6_001688"
     CDS             11244..11765
                     /locus_tag="ACG8E6_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFS5062103.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11844..12398)
                     /locus_tag="ACG8E6_001689"
     CDS             complement(11844..12398)
                     /locus_tag="ACG8E6_001689"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS5062104.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12651..12926
                     /gene="rpsP"
                     /locus_tag="ACG8E6_001690"
     CDS             12651..12926
                     /gene="rpsP"
                     /locus_tag="ACG8E6_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFS5062105.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12938..13189
                     /locus_tag="ACG8E6_001691"
     CDS             12938..13189
                     /locus_tag="ACG8E6_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFS5062106.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13313..14104
                     /locus_tag="ACG8E6_001692"
     CDS             13313..14104
                     /locus_tag="ACG8E6_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062107.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14274..14795
                     /gene="rimM"
                     /locus_tag="ACG8E6_001693"
     CDS             14274..14795
                     /gene="rimM"
                     /locus_tag="ACG8E6_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFS5062108.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14785..15546
                     /gene="trmD"
                     /locus_tag="ACG8E6_001694"
     CDS             14785..15546
                     /gene="trmD"
                     /locus_tag="ACG8E6_001694"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFS5062109.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15682..16029
                     /gene="rplS"
                     /locus_tag="ACG8E6_001695"
     CDS             15682..16029
                     /gene="rplS"
                     /locus_tag="ACG8E6_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFS5062110.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            16339..16581
                     /locus_tag="ACG8E6_001696"
     CDS             16339..16581
                     /locus_tag="ACG8E6_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFS5062111.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(16669..16815)
                     /locus_tag="ACG8E6_001697"
                     /pseudo
     CDS             complement(16669..16815)
                     /locus_tag="ACG8E6_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            16872..17186
                     /locus_tag="ACG8E6_001698"
                     /pseudo
     CDS             16872..17186
                     /locus_tag="ACG8E6_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            17215..18474
                     /locus_tag="ACG8E6_001699"
     CDS             17215..18474
                     /locus_tag="ACG8E6_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5062112.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            18527..19423
                     /locus_tag="ACG8E6_001700"
     CDS             18527..19423
                     /locus_tag="ACG8E6_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5062113.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            19434..20303
                     /locus_tag="ACG8E6_001701"
     CDS             19434..20303
                     /locus_tag="ACG8E6_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5062114.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            20313..20988
                     /locus_tag="ACG8E6_001702"
                     /pseudo
     CDS             20313..20988
                     /locus_tag="ACG8E6_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
BASE COUNT         6942 a         3614 c         4296 g         6180 t
ORIGIN      
        1 agattacaaa tagtatcgta tttcgcaatc ttaaaattat cttcttactg tgaataagat
       61 aataaaattt gtatttgtcc tttttaggta acttatttta atctatatac ctttctggat
      121 caacgaaagt atactttata tagtcataac gccgatggta actatgcact gtaacgataa
      181 tgctgctaaa atatcttttt acgaaaaaca tcacaaggaa aaacttaact atggtattat
      241 ctaaacataa ttttttcggt atttaatatt taattaggag gtaatatttt gcttttttat
      301 agtaatttta tcttaatagt agcgatatta ttacttctga atatatggat ttttgatagg
      361 agtagaaatg caagtatagg ttttagaact aaacgaagtc tgtcttctaa aaaaaattgg
      421 gtatactcac aaactatttt ttatggagga attgttttaa ttagtttact ttcttcaaca
      481 ctttattctt tgaatattat agatgtttcc acctcaaatt caatatctat tattggaatc
      541 attattgctg caattattac acaactgttt ttagtgtttg gagagaagaa aagaagtaaa
      601 aaatagaaaa actagtgata gttttggtga tataagaaaa acatatatgt ataaaaagaa
      661 gaaagtagct tctctttgta catatatggt ttgtttttat ggatgccgag cttttttata
      721 agataaggct accgcccata acgacccaag gatttgtgga actaagtaaa tacctgcaag
      781 aagcaagatc agctgggtcg gttttaaagg atgatagaag taacgccatt cagtaaaatt
      841 ggcgcaaaat aaataggaag tgacagtaga tataatattg gcaatgaata aagatataat
      901 acgcctgttc ctttgaaaat aatatccagc aaaaagagat aaaaatatca atgtgatgaa
      961 cccgcttacg gaatgataac gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa
     1021 agataaaagc aacggaaggt ttttccagca agtttttatc atctttcatc accacctaac
     1081 aagtgttatt attttgttag ttttattgta aattgaatcc tgcaataatc aattactttt
     1141 tcattatttc tagattaaat gaataaactc tcatgccatt atctctaaaa aaaaagtttt
     1201 tccccctaat ttttttagat agaaaattgt atagtatgat gagagaaaaa gtagtttggg
     1261 ggaacgttaa tgagaagaaa agcagtaatc gatcggcttt atttattttt ttggtttttt
     1321 cctatcgttt tgctaccttt tacatgggta aatgctgttg tacaaggtgc ggagatatta
     1381 ggcctgatcc aaggaagcgg attagtggtt tctaggaatt ttccagcttt gactgttttc
     1441 tatttagtga taagtgtagg tctcttatta ccaaaatctc tgatcaatca ctacaattgg
     1501 cagctgcttt acttttcttg cttattagta tttacatcta tgttttcttt gctgcctcgc
     1561 ttgattatcg gccctaatgg tttgagcgag agtggattga aggattcagc ttattcctcc
     1621 atgtatttct tgacgatcaa accatggttc tatttagtaa ttgcctcatt tattcttgca
     1681 acattttttt acttctggac agaaatcagg ataaaaagtg aacgatctgt atctataaaa
     1741 aataactatt gatggtggat catcagtaaa aaagcatatg agctgcgtaa aatgcatgtt
     1801 tctcatatgc tttttttatt attatgtaaa gaatgcgaaa aacgtttaca tgatttttac
     1861 ataacttaac tatattttta cagcaaccat acgagggatt tacagtcgac tgataaactt
     1921 tagttgtaaa taagaaagga gcaataaaat gaaaaaagta ttgtttactc taggtatgat
     1981 gggaattctt ttagccggat gcggctcagg atcaggaaat acagatggct ctgccacaca
     2041 aaataattca agtgactcaa atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt
     2101 acagccgtta gtcgatgcag cacaagaaag ctttgtacaa gaaaatccaa attatcaaat
     2161 ctctgtacaa ggaggaggaa gcggaactgg tttgagccaa gtcgaagctg gagcagttac
     2221 tatcggaaac tctgatgtat ttgcagaaga aagagacgga gttgatgctt caaaattagt
     2281 ggatcataaa gtagcagtgg tcgggatggc accaatcgtc aataaagata cagatgtcaa
     2341 agatatcacc aaacaggaat tgattgatat ctttacagga aaaatcacta actggaagga
     2401 agttggcggg aaagatcaaa aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg
     2461 cgcgacattc gaaaaatggg gactggatgg tgctacccct gtgcagtccc aagaacaaga
     2521 ttcatcagga acagttcggc agctggtaag tcaaacacca ggagcaatca gctatttagc
     2581 tttctcatat ctcgatgatt ccacacaagc actaagtatc gacggtgtag aaccaaaaga
     2641 agagaatgta gcagataaca gctggggaat ctggtcatat gaacatatgt acacgaacgg
     2701 aaaaccttcc cctgaagtcc aaaaattctt agactacatg atgacagaag aaatccaaga
     2761 aggacctgtc aaagaattgg gctatttacc aatcacaatg atggaagtag aacgtgatca
     2821 cgaaggaaat atcaaataac tagcagataa acatgccacg gtagtcgtta ctattaagga
     2881 aaaatcaact atgtttcccc acatatacat ttttccttta attgtgaaaa atagttcctc
     2941 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttattcga
     3001 aaaaaagagc tacgacatag aactgtcgta gctttttttt tgctatatga caggaatgaa
     3061 aggtgtttgc aagcttaatg tgaggaacac tttccatatc tgttctatac tggataggag
     3121 aagtttggaa atatccagaa aacgaaatca atcgaggtag ccacatgaag aataaatgga
     3181 taaatcgaag taaaaaaaag aaagtaacac aaagatcatc agataaagtc atccgtcttt
     3241 ttccatcgta taaagaagga ttaacggcag atcaagtcaa ggaacgaata gaaaaaggtg
     3301 cagcaaataa ttcagtcgat cccacattca aaacaaatca gcaaatcgta ttggaaaaca
     3361 tatttacgta tttcaatttg atctttctta tcttagccat tttgctttgt ttagtagaat
     3421 cctataagaa cttgactttt ttaccagtta tcatagccaa tacaggaatc gccatttttc
     3481 aggaaattcg atccaaaaag atcttggacg aactaaatgt tttaaataca gcaaaagtca
     3541 atgtcatacg agacgggata gaacaaacaa tagatataga agaccttgta atagatgaca
     3601 ttgttatttt agagactgga catcaaattc cagcagatgc agaggtcgtg gaagggaaac
     3661 tgcaagttaa tgaggcttta ttgacaggcg aagcagatga aataaccaaa gaaataggcg
     3721 atccattaat gtccggcagt ttcattgttt ccggaaaagc ttacgcccga ttagacaaag
     3781 ttggagcaga ttcgtatatt tcccagctga cagtcaaagc caagacaatg ggagaaggag
     3841 aacagtcgga gatgattgct tctcttaatc aattgatcaa atggattggg atcatcatta
     3901 ttcctatcgg tatcctgctt ttttctcaaa gttacttttt taatggaaac acgatcaaag
     3961 aaagtgtcgt tgcaatggaa gcagcattga tcggtatgat acctgaagga ctgtacttgc
     4021 tgacgacgat tgcattagca ttaagtgcga ctaaactagc aaagcaaaga gttcttttac
     4081 acaatatgaa aagcattgag acattggcaa gggtaaatgt cctttgtgta gataaaacag
     4141 gaacgatcac agaaaataaa atgtctgttc aaaaagtgat cgtctctaaa aaacaagaaa
     4201 taatcagtca gtcagatcaa ttggaagcat gcattgctga ctatgcaaaa gcgatggcag
     4261 gagacaatgc aacaatggaa gctgttaaag catattttac aaaaaccact gatcaatcct
     4321 atcaccatgt tattcctttt tcttctgtcc aaaaattcag cagtgtagcg ttggcagaca
     4381 aggtgtatgt tttaggagcg cccgaaatgg ttttgcgcga tcaattgccg gaatatgcgg
     4441 aagagtttgt ttctttcgct gaaaaaggct atcgcgtgtt ggtctttggt gtgtacgatg
     4501 ggattcttga agaaccagta ttgacagaag cagtcacacc attaggctat attctgattg
     4561 ctaatcctat cagaaaagaa gcaagagcca catttgatta cttcaaaaaa cagcaagtag
     4621 ccatcaaagt gatttctgga gataatccgc tcaccgtatc caatgtagct gtccaagcag
     4681 ggatttcaca tgctgatcag tacgttgatg catctaaatt atcagaagaa gaatatgaag
     4741 cagctgtcga aaaatataca gtttttggac gcgtcaagcc agaacaaaag aaaatcttcg
     4801 ttcaattgct aaagcgtcgt aacaatacag tagctatgac aggtgatggc gtgaatgata
     4861 ttctagccat gaaagaagca gactgcagta tagcgatggc ttccggaaat gaagccgcca
     4921 tgcaggcctc acaagtggtc ttacttgatt ctgacttttc acgcatgcca gaagttgttg
     4981 cagaaggacg gcgcgttgtg aacaatattg agcgatcagc cagcttgttt ttagtgaaga
     5041 atattttctc ctttttgctc tcactgtttt cagtgatctt tgctctgacc tatcctttag
     5101 aaccatcaca aatcacattg attagtttgt ttactatcgg acttccatct ttcttgcttg
     5161 ctctagaaga aaacaaaaag cgaatcagag ggaaattcat catgaatgtg atggaaaaag
     5221 ctgtgccagg tggactaaca gatatgatcg tagtcggtgc gttggtgatc tgcggggtga
     5281 ctttgaatct aaataaaaca gatgtttcta cagcatctac catgcttttg attgcagtag
     5341 ggttcctcgt tctctataaa atctgttcac cgctgaataa attcagaagt cagatcattc
     5401 ttttctgtgc tagcggtaac tttttttcgg tcatcttttt gcataagctg ttttcaatca
     5461 ccggtatctc agctgtatca cttttgctgt tgagcattct atttttctcg gcagattcga
     5521 tttttaggca tttgaccaca ttggtagaat atttgtttga acgtaaaaat gaaaaagagc
     5581 aaaagccgtt tagtcgcgta gttaaaaaat ttctatcgat atttaaacga gaaaattgac
     5641 gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt cgaagcagga
     5701 tgagtcatcc aactttaaaa cgcacgagca gttgctcttt tttacatttt cggaatggta
     5761 acggtgaaag tcgaacctgt tccaagatga ctgtctacag taatcgtacc atgaaggagt
     5821 tgcacgtagt cttttacaat cgctaaccct aatccggtcc cgcctgaatg ccggcttcgc
     5881 gccttgtcta cgcggtaaaa acgctcaaag atccgttcct gatcctcaat gtcgatacct
     5941 ataccaaagt cttggacaga aaacgataga tcatctgttg cctgatagcg gataataatc
     6001 tcgctgtcgc tttttgaata ttggacagca ttttcaatca gatttttgat aattggataa
     6061 aggatatctg ttttagtagt aaatggttcg ttctttccaa tcaatcgaat cgtcaaccgt
     6121 ttcttttcga tgattggctg ataattttga acgatttgct ggaaataagg ttctgtataa
     6181 attgtctgga tctcgtaaga attctcgcca tcttttgaca gctggatgat ttcccgaatc
     6241 agtttatcta accggatcgc atctttttgc atgatctcaa gaaaagaagt cagagtttgc
     6301 gggtcatctt ttgcaccatc cagtaaagtc tcggtaaaac caatcagtga agtcacagga
     6361 gtttttaatt cgtgagagac gttgccaacg aaatcctttt gtagcttttc taaacgtcgc
     6421 actttcgtta agtcatacgc cacccctaga atctgtccgg taccttctga attagtaaag
     6481 tagcgcaaac tcaagtctaa tgtctgccga ccttctgtaa gggtgatctc ctgatggatc
     6541 agcggatgct caggagtcac ttgatgaatc agctgaatga gttttggctc ttgaatgact
     6601 tctgtgtacc gctgttcagg ttgatagctt accacgttta aatgtagctg catctttgga
     6661 ttaagcagta gtaaacgaga atcagcatca tcaatcagga atacgccaat catcagttcg
     6721 ttcagtaacg tatataattg ctcttctgtg gtagtatacg cccgatacat cttactcatt
     6781 tgttcgctca acgcattgat cgtctgatac aattcttccc attgatcggg tgtttgcatg
     6841 attacttccg cctgattagg attttgcacc atttttttta gtacgggcag cacggttttc
     6901 aatggctggt ttttttgata gatcagataa tagatcattc ctgcaagaac aaggaagaaa
     6961 gacagaaaga agaaaaagac ccaacggcga aaactctcag ttctcggtaa aaaaccgctt
     7021 gtcggttctg cgatacgtaa aatggcttct aactggccac tttttttgac tggtaatgcg
     7081 acatataaaa gatccccatt caaagtagta cttttgcgca aagcgctacc taggttccca
     7141 ccattcaata cggctttgat ttcgggacgg gagtttcgct gttcattcaa tgtttcattg
     7201 tttgtgtcaa ataagatctt tccagtagca tccattaaag taatccgttc gttcgactga
     7261 tggacgaatt cttccaaagc agttttgttt tgcgctgctt ggaggttttc gacatctaat
     7321 tgccggatca gcagttgtgc tttttttgtc aggtagcttt cttgctgttc cacgatctgc
     7381 tgttgaaaat aatgggaaat caattgccag ccaccaaaaa acaaactaac taaaagaata
     7441 agaaagaaga gacgttcata aatttttttc catttcatcg tttgggttcc tgaaacttat
     7501 agccaaatcc tctaatagtc accaagtact ttgggtgttt tggatctcgc tcgatttttt
     7561 ctcgcaaatg actaacgtgt acatctacga ttcggctttg tcctgcgaag tcaaaattcc
     7621 aaatgcggtc tagcaatgtg tctcggtcga tcacacggtc ttttcgtttc atgaaataaa
     7681 caagtaattc aaattctttc ggtgttagtt caatcagatg gtcgtctacg gtcacttgat
     7741 aattggtcaa atcagcagtg atttgtccaa tcgatagata ttcaggctca gcttcatctt
     7801 ttgattgctt acgaggttca attcgtctga atattgcttt catacgggct aatacttcgc
     7861 gaggactaaa tggtttggtc aaatagtcgt cagcacctat ttctaggcca atgatccgat
     7921 ccacttggtc atctttagcc gtcagcatca aaatcggtgt ttcatttttt tcttgacgca
     7981 gccgctgagt gatctccatg ccatccatcg ttggaagcat cacatctaaa atgatgaaat
     8041 caaatgattg ttctaatgct aattctaatc cttcgcctcc atctgtggca ctggttacct
     8101 tgtagccttc tttttccaaa ttgaacgtca agagcgttac aatagaaggt tcatcatcaa
     8161 caactagtat ttttttcatt caaatctcct ttaaataggt ttcattctgt ttatatttta
     8221 gcatagaatc agtacaaagg gctattttac aaaagaaata aatggtataa taggaaggca
     8281 ctttgctgaa tagaaaggaa gagcagtcaa tgattggaat cagtgcatgt ttaggcggtc
     8341 tggcctgtcg ctatgatgga cagaaaaaag aagtaccaga attgatggaa ctagttgaaa
     8401 ctggagaagc tgtcatggtc tgtccagaag tgatcggcgg attaccaatt cctagagatc
     8461 ccgctgaaat cattggtgga aatggattcg atgtgtggaa agatcaagca aaagtatgga
     8521 cgacaagcgg cgaagatgta acggaagcgt acaaaaatgg cgcgatcaaa gcctatcaaa
     8581 aattgcagga aaaacacatt gatctagtca tcatgaagga aaaaagccct tcatgcggaa
     8641 cccattcgat ttatgacggg agtttttccg gaataaaaaa aaatggtgcc ggagtggcta
     8701 ctgcttattt tattcagcaa aaaatgactg tgctctctga tgaagaatgg ctgaaaacta
     8761 gaggtgagca gaatggaaat cgaaaagacc aatcgaatga acgcgctctt tgaattttat
     8821 tcgaccttat tgacagaaaa acaaatgaac tacatggaat tatattatgc agatgatttt
     8881 tcattaggtg agatcgcgga agaatacgaa gtaagccgac aagcagtata tgacaatatc
     8941 aagcggacaa gcaagatctt ggaggattat gaaaaaaaac ttcatctttt ttctaattat
     9001 attgttcgag aacaggtatt ggaaaatatg actgcatata ttacagataa gtacccggaa
     9061 gataaaaaac tacttgaata tgtacagcaa attcaagcaa tcgaagaata aataaaagga
     9121 atgaagaaaa tatggcattt gaaagcttaa ctgaacgtct ccagcaggcg atgggaaaaa
     9181 taagaaaaaa aggaaaagta tcagaagctg acgtaaaaga aatgatgcgg gaaatccgcc
     9241 ttgccttact agaagccgat gttaacttgc aagtagtcaa agattttact aagcgcgtac
     9301 gcgaacgtgc ggtaggggca gaagtgctgg aaagtttatc acctgcacaa caaatcgtaa
     9361 aaatcgttga tgaagaattg acgatcacac ttggttctga aacagcagag ttaaataaat
     9421 caccaaaaat accaactgtt atcatgatgg ctggtcttca aggggcaggt aaaacgacct
     9481 ttactggtaa attagccaat tatttaaaga aaaatgaaaa tgctcgtccg ctcctaatcg
     9541 caggggacgt atatcgacca gctgcgatcg accaattgaa agtattaggt caacagctag
     9601 acgttcctgt atttgatatg ggaactgaag tcagcccggt agagatcgtc cgtcaaggga
     9661 tggagttagc aaaagaaaag aaaaacgact atgtattgat cgatacggca ggacgtctgc
     9721 atatcgatga aacattgatg gatgagttga aacaaatcaa agaattgacg caaccaaatg
     9781 agattttgct tgttgtcgat gccatgactg gtcaagatgc cgtcaacgta gctgatagct
     9841 tcaaccagca attaggtatt acaggggtcg tcatcactaa acttgatggg gatactcgag
     9901 gcggggcagc actatcgatc cgctcagtga caggcgcacc aatcaaattc atcggttctg
     9961 gtgaaaaatt gactgactta gaagtattcc acccagatcg tatggctagc cgtatccttg
    10021 gtatggggga tatgctgaca ttgattgaaa aagcgcagca agactatgac gagaaaaaag
    10081 cggaagaact tgcacagaaa atgcgggaaa attcattcga tttcaatgac tttattgaac
    10141 agcttgatca agtgatgggt atgggcccac tggaagatct aatcaaaatg atcccaggaa
    10201 tgaatcaagt ccctggaatc gaaaacgtca aggtagatcc aaaagatgtt gaacgcaaaa
    10261 aagcgatggt ttattctatg acgccagcag aaagagcaaa tcctgatctg ttgaacccaa
    10321 gccgtcgtcg ccgaattgct gccggttctg gaaacagtgt tgttgaagtg aatcgaatga
    10381 tcaaacaatt caaggaatca agaaaaatga tgcagcaaat gtccaaaggg gatatgaaca
    10441 tcccaggtat ggaccaaatg tttggttcag gcgtcaaagg aaaactaggc aaaatggcga
    10501 tgaatcgaat gatcaagaaa aataagaaga aaaagaaaaa acggaaataa gatgaaaaaa
    10561 acagcttcgg tacagactga aaatgtctac tgccggagct tttctatcag caatttttat
    10621 tctttactaa tacaattact ctaaacgaag aggtggcaca gatgagatgt ccgtggagtg
    10681 aacaaacaga aagtatgcgg caatatcatg atactgtctg gggtgttcct gaatatgatg
    10741 accaaaagct gttccgaaaa ttgatgcttg atattaatca agccggattg agctggcaga
    10801 cgattttaaa taaaagtgaa gcattcgatg aagcatatga tcgattcgat attaaaaaag
    10861 tcgcaaatta tggagaaaca aaaatcagtg aactaatgga aaacaaagga attattcgta
    10921 accgaagaaa aatcgaagca gccgtccaaa acgccaaagc agtcttgaaa attcaagaag
    10981 aaatcggcag tttttcagag tatctttggt cgttttctaa tggaaaaatc gttgattctt
    11041 cttatcattc acaagaagaa gtcccaacaa caagtcctct ttcagatgaa ttagcaaaag
    11101 atctaaagaa aagaggattt aaatttatcg gaagcacaac aatctatgct tttttagaag
    11161 ctgtcgggat catcaatgat caccttgaca gttgtttccg caaagaagag gtcgtacgta
    11221 tcggcagaaa gggcgaataa agcatgcgaa aaatcatatt ttatggtgct ataagtttag
    11281 atggctattt ggcaacgaaa acggatgatc ttcaatggct ttttgacact ccaacaggta
    11341 agcaaaccac ttatgaagct ttttatgaaa caatcgatac gaccatcatg ggcagaaaaa
    11401 cttatcagga agcaaaaaaa tatttggaga ccgaagcaat ttatccagag aaaaagaatt
    11461 tcgttttttc cacccacgca caattacaat tgccagatgc tgaggtcgta aacgaagatc
    11521 ctgttgcttt tctaaaaaat ctacaaatga cagaaggaaa agatatctgg gttgtgggtg
    11581 gcggtcgatt gatcaaaccg ctattggaac atcaaatgat tgatgaatgg tatatccaga
    11641 ttacccctgt attattaggg gacgggattc ctttattcca aaaaggaaca tatgaagcgc
    11701 gttttgaaat gctagacacg acacgattcg gtgaatttat cgaattgcat tatgtacgaa
    11761 agtagaagta aataaaataa aaaaggcaac tatatgcaga aatgtcctgt acatagttgc
    11821 cttttttatt accgttatgc tttttactct gttaatttgt tagaaaaatc tagatacgca
    11881 tctaaaatcg tttggaagaa acttaatgtt ccttcaacga tattaccatt ttcatcaatt
    11941 agattcgcaa cgtttccgat atatgcttcc ggttgctgca aggttggcac attcaaaaag
    12001 acaagtgatt gtcgtagatg gtggtttgcg ccaaagccgc cgatcccacc tggtgatgca
    12061 gtaacgacta aacctggttt atgatcccaa acactgtgtc cataaggtct tgagcctaca
    12121 tctaaagcat ttttcaatac tgctggcaca gaacggttgt attcaggagt aacgaaaacg
    12181 acaccgtcta gtcccctcac ttcctcacga aaacgagtcc attcagcagg agcctgttct
    12241 ggagtttcga gatcctcatt gtaaaatggc agatcatcga tttttacaaa tacactttcg
    12301 tacccttcag gaagcaattt cgcaaaagct tctgctactt ttttgttgta agaatctttt
    12361 cttaaactac caataaaaaa tccaattttt tttgtcatac gatttttcct ccttcaaact
    12421 atctataaca atcttacaaa aaataagtaa ctggagcaaa taaaaacact tactgacaaa
    12481 agctacatag aagatagatt acttattttt aacaaaaaag aagtctaaaa tcttctattt
    12541 cctaaaaaaa atcgaattta atcaagctgt aaagaaaaaa agctttacag ggtaaaaaaa
    12601 aactgttaca ctacatcttg tgaataaaac aatggaggtg gaaataaata atggcagtta
    12661 aaatccgttt aaaacgtatg ggttctaaaa agagtccgtt ttaccgtatc gtcgtagctg
    12721 attcacgttc tcctcgtgat ggacgtttca tcgaaactgt aggtacttac aatcctttga
    12781 aagaccctgc agaagtagtt ttaaaagaag acttagttct tgactggtta tcaaaaggtg
    12841 cacaaccttc tgacactgta cgtaacatcc tttcaaaaga aggcgttatg caaaaacacc
    12901 acgaagctaa attctcaaag aaataaggtg accggatatg aaagatttaa gcgacttagt
    12961 cttaacaatc gttcgtccgc tagtcactta tcctgatcaa gtcaagttag acgtcgtaga
    13021 gtcgaaagac ttttacgagt acaatctaac agtggctcct gaagatattg gccgtatcat
    13081 tggaaaacaa gggcgtgttg ctaaagcaat ccgcacaatc gtttacggtg ttcggatcaa
    13141 tgcaccgaaa aaagtgcgtt tgaatatcat cgatgataag gaaaagtaaa taaacgtgct
    13201 gtactcgaat tttcgagtgc agcttttttg tttttttatt aattgaagaa atgattattt
    13261 ggcaaaacaa gcaagaaagc gtatccttat aggaggaggg agtgaacagc caatgagaaa
    13321 gaaaatgtta tttattccat ttgctctatt gttaagcgct tgtagttcgg ttactcaaga
    13381 atcagacaat acacatctat caagcagtat atctgaaact gttaccagtc cagaaaaaag
    13441 tagtaccaaa acggctacca ccactgaaca aacaatggct acctcaaata atgagaaaaa
    13501 gactgcttta gaccagttga aagaacaaca gccaaatgtt cccatgcctc tagatgtacc
    13561 agtctcttca ggctatctca atatcgcagc gacacataca aagcaaggtt attctatttt
    13621 atattacaga acagatcgtc ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc
    13681 tattgcaacc tatctatatc aatatgggtt tgcttctagt caagaaacaa tccaagtttt
    13741 gcagcccttt gagatcgaca caaatggtca gcaagtggac ttaggttctc gaatcacagg
    13801 gtatcagcaa ggtgcagcag gttcaagttt tcttgaatgg caagaaggaa actggcgtat
    13861 tcgaatccgt gggaataata tagaaggcca agatccctta ctattagcta aagaaatcgt
    13921 agcttatttg gaagaaaaca gtctaccagc acctgaacaa tttggtaaaa ttactgttga
    13981 tatgggtgat acgacgaatc gagccgtaga agtaagctgg caagaaccga aaaatgccta
    14041 tacgatcact catcaagatc ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt
    14101 gtgaaatcag cgcgctgatc tgagcccatc atttaactcc aagtcacagg aagtaatcaa
    14161 aaatagcacg ccgcattttt gcataatttt ggcttaatac cttaaaagaa aactagaatt
    14221 gcctcttcac acatgctaaa ataaaacaga caaaaaagaa atggggatat aagttgactg
    14281 aatacttgaa tgtaggaaaa atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta
    14341 tctcaacaac ggatttccca gaagaacgtt acaaaaaagg aacggtgtta acactattcc
    14401 aagacggaaa agcacctgtg gaattaacag tcaaaagtca tcgcaaacat aaaaattttg
    14461 acttattgag ttttgaagga catccttcta tcaatgatgt ggaaaaatat cgggatggca
    14521 tattaaaagt gtcaaaagac aactctgtag atctagctga aaatgaattc tattaccatg
    14581 agatcatcgg tctgcatgta ttagaagatg gaactgagtt aggtaaagta aaagaaatct
    14641 tgtcaccagg tgccaatgat gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc
    14701 ttccatatat cgcttctgtc gtcaaagaag tcgatttaga agctggagtg gtccgtgtag
    14761 aaattccaga aggactgatt gacgatgaag attgatgtat tgactttatt tccaagaatg
    14821 tttgaaggtc ctatggggga atccatcatc ggtaaggctg tagataaagg attattagat
    14881 atcaatatct ctaacttccg cgattattca gataacaaac atcagacagt agatgattat
    14941 ccatacggcg gtggagcagg tatgctgctc aaagtccagc ccatctatga taacatccaa
    15001 gccattgaaa aagctgcacc ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt
    15061 aaacgattcg atcagaaaat ggcagaagaa ttttcacaag aagaacactt agtattcatt
    15121 tgtggtcatt atgagggata tgatgaacgt atccgcacat tagtgacaga tgaagtttcc
    15181 ttaggagatt atgtccttac tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca
    15241 gttcggcttt tgccagatgt gttagggaat cagacttcag ctcaaacgga ttcttattca
    15301 accggtttat tggaacatcc ccaatatact cgcccggcag aatataaagg gatgaaagta
    15361 ccagaagtat tgacaaatgg aaatcataaa ttgattgaag aatggcaatt aaaagaatca
    15421 ctaagaagga cctatcaacg tcggcctgat cttttggaaa cacttgaatg gacacctcaa
    15481 atgacgaaat ttttagaaga aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc
    15541 aagtgacttt ttctaaaata ttctttacag taggttgata atatgttagt attttatggt
    15601 gagtgcaaaa gcacttaact attacgatat tccgctatga gaaaagctca taagaatatt
    15661 tggaataagg agaagaaaac catgaatcca ttaatcgaag aattaacaaa agaacaatta
    15721 cgttctgaca tcccagcgtt ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt
    15781 gaaggtactc gtgaacgtat ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct
    15841 ggaatcagcg aaacttatac agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca
    15901 ttcccattgc atacaccacg tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt
    15961 cgtgcaaaat tgtactacct acgtgcatta cacggaaaag ctgctcgtat caaagaaatc
    16021 cgtcgttaat cattgttatc agactgagaa agaacccttg gtattcaagg gttctttttt
    16081 cttagcatgt aatttgaaca ccctgatcca gtttttttct ttaattattg gtaggggaga
    16141 ataatggatt agaataaatc tacctataag tagtagtaaa aaaaagagca ctatgataat
    16201 atctttctta agagtaaccg ctttaataaa aaatccagaa tattctaaca taaatggagt
    16261 attcttagaa tcaaaaggag aaagaagatg gtttatactt atacggagca agaaaaaatg
    16321 aaagtttgag acacattcat gaatgatgga aaagtaaaac aagtaccaag ttcaacaaaa
    16381 aagaaaaaca tcctattgaa agaagtcttg aaacgctttg accatggagt gacttataca
    16441 gaaacagagg ttaattcgat actgctgaac gtattttcta gtggcgatta tgtcgaacaa
    16501 agacgttatc taatcacatt tggctttttc aaaagaagtt cagacggtcg ggcatatcag
    16561 atgatgggaa ttgaaaatta gaagttttaa tttatactca ttgaaaaaag ttcaagtatg
    16621 cgatattggt tctacaccgc atacttgaac tttttttgtc atctgtatct ataatacaaa
    16681 gccaagaacg ttattcaaaa tatggatagc tatgataaaa gctaatgagc gattcgcagt
    16741 taaataagca atcgtcagta aaatcgttcc aggagcgtac atgaaaaaat caattggatg
    16801 cgttacatga ataaagataa ttctctttta tttgtacgaa taaataccaa aacgtacctc
    16861 ctaaaattag attcaaagaa gatagcaaag aggaaaaaga tagttctgca gcagctatag
    16921 cggcatgtgg attgctagaa ctttccaaac aactgcctgt ttcagatcct cgtcatttcg
    16981 attacgaaga gatagcaatc aaaatcttgg aagaattaca gaaaaactat acaacgaaac
    17041 gagttaacgg atcaaacggt ctgcttctcc atgccgttta tgataaaaac agcttaaaag
    17101 gtgtagatga atgtgtgatc tggggcgatt acttttatgt agaaggaatc actcgtctag
    17161 ctaaaacatg gtactgttac tggtgatgta taagagaaga ggaggattca tataatgaag
    17221 cgatggaaaa aaatgatgat tggatcaata gttttaggaa tgtcactcgt atttggtgct
    17281 tgtggtaatc aagaagacag tagtgaagaa tcagcaaatg aaattaccgt ttcgacatgg
    17341 aattatgaaa caacacctga attcaaagcg ctctttgaag cattcgaaaa gaaaacggga
    17401 atcaaagtga aagcagtaga tattgcttca gatgattatg atacgaaact caccacgatg
    17461 ctttcttctg gagacacgac agatgtattg acgatgaaaa atcttttgtc gtattcaaat
    17521 tatgctttac gagatcagtt agtcgatcaa acagaacgaa ttagagaatt agacactgat
    17581 gctgctgaag aaacatatga aatgtatgat atcgatggaa aaacatatgc attgccatat
    17641 cgaacggact tttgggtact atattacaat aagaaaatgt ttgatgatgc aggaatcgaa
    17701 tatccaaaaa atctaacttg ggacgaatat gaagatttag caaaaaaact tagtaaaaat
    17761 gacggacaag tttacggagc atatcaacat atctggcgtt cgacaatcca agctattgca
    17821 gctgctcaaa acgaggcgaa tcttgttgaa ccagattatc aatatatgac tgattattat
    17881 gatcgagcat tgaggatgca aaaagaaggc gcgcagatgg attttggcac ggctaaatca
    17941 acgaaagtaa cctatcagtc tcaatttgaa gaacaaaaag cagcaatgat gtacatggga
    18001 acttggtata tggctggcat actcgcaaac aaagaagcaa gtaagacaga agtagaatgg
    18061 ggaattacag ctattcctca gaagaaaaaa ggagaatcgg tcacgacgtt tggctcacca
    18121 acagcatttg ctgtcaataa aaactcaaag aaacaaaaag cggcgcaaga atttattgaa
    18181 tttgcagcca gtgaagaagg agcaaaagta ttagcaggag taggagtagt tccttcatat
    18241 cgtacggatg agattgatca gctttatttt aatctcgctg gaatgccaac ggatgaaatt
    18301 tctaaaaaag ctttttctcc tgatgaaatc aaacttgaat ttccgattga tacgtatggg
    18361 cctgcaatcg ataagatttt acaagaagaa tatgacttag ttttggttgg agatgaaacg
    18421 ccggaaaaag gaacggtaaa tatggagaaa cgtgtagctg cggaaaaaga ataatcatga
    18481 aaggagagaa gccaagagat aaaacactct tggtttcgtc atcgaaatgg agaatagtat
    18541 aaataagaaa gctacaaatc atttgcgtag aaaacgaacg tggatcgctt attcgtttat
    18601 cgccccgaat ttcattggtt ttttcgtttt tacattaata ccaatcctgt tttcgcttgt
    18661 actagcattt atgaaatggg attctttttc aacgccagaa tttgctggac tagataactt
    18721 tcgtaaaatg gccaacgatg agacattttg gatttctttg aaaaatactt ttttatatac
    18781 cgctgctgta gtgcctggca cacttgtttg ttcgttaggg ctagctttac tactaaatca
    18841 gaaaattaga gggatgaagc tttttcgtac gatatttttc tttccgtatg ttacttcctt
    18901 agtagcaatt gctgttgttt ggaatatgtt gtttcatcca acagtgggac ctattaatat
    18961 ctttttaaga cgatttattg aacatcctcc aggttggact tcaagttcaa attgggcgct
    19021 ttgggcaatt gtaattgtca gtatttggcg agggatgggt tattacatga ttctatacct
    19081 tgcaggacta caaagtatcc aaaaagaact atatgaagcg tcttcgctag atggagcaaa
    19141 ccgttggaaa caatttttga atgtgacatt accttcgttg agaccaacaa ccttctttgt
    19201 aacgatcatg cttgtcatca attgtttcaa ggtgtttgac cttgtgcaag tgatgacgga
    19261 tggcggccct ggtcgagcga caaatgtgct ggtttaccaa atctacaatg aagcattcaa
    19321 aaaatttgat tttggttatg cttcagctat ttcacttgtt ttgttcgtaa ttgttttggg
    19381 cgttacggtt gttcaatttt cttggaataa gcgtcgtact taggaggtgt gatatggaag
    19441 agagtgttcg aaatgaaaga aaaaaagcaa aaattcgttc aaaaaacatg ccctttattc
    19501 aatggttggg tatgattctg ctgattgtat tagctttatt gacgatcatt ccttttttat
    19561 ggatggtttc tgcttcattc aaagcaaata atgaagtgtt aacgattccg attcaatgga
    19621 tccctaaaac ttggcattta gagaattatc tggttatttg gtctcgtatt cctttagtga
    19681 cattttttaa gaatacagct tttttaagcg ttgtgatcac gttgattcag ttgtttactt
    19741 ctagttttgc tgtttacggg ttttctgaac taaattttaa agggcgcgat attttatttt
    19801 tagcatatat cggcacgatt gctgtccctt ggcagtctta tatgatcccg cagttcatta
    19861 tgatgagaaa agtgggttta acagatacgt tgtggtcgtt ggtgctatta caagcgttca
    19921 atgcttttgg tgtgttcttg ttgaagcaat attacagtag tatcccagat tctttatgtg
    19981 agagtgcgcg gatcgatggc ttatcagagt gggaaatcta tcgcaagatc attttacctc
    20041 ttacaaaacc ggcgattgcc agtttgacga tcatcacatt cgttaataca tggaacgatt
    20101 atatggggcc attcatttat ttgtcttcta cggaaaataa aacaatccag ttagggttga
    20161 aaatgtttat tgggctattt gatgcagagt acgcattaat catggctgcc tcagttgttt
    20221 ctgttcttcc tgttgctttt atatttttag cgatgcaaaa atattttgta gaagggatcg
    20281 cttcatcagg aatgaaggaa taggaggtga ttatgaaaga gcagcgtttt gacaaaaatg
    20341 tttatatgga attcttttat tggttgtatc aatcattggc aagtagcctt tgtttttggt
    20401 gtgtcaatat cttgtatata gcagctgcgt tccttttgtc gatagatgga aggaatgtct
    20461 tttcctttat tttttcttta ctttttattg gtccaggttg catcgtgatg ataggtatga
    20521 cagaagaatt agcaaaagaa aaaagttttt caccagtacg tttgttcata aagacgtttc
    20581 gccaattttg gctaaaggga tttttttatt ggctatttgc ttggatagtt agtgtgataa
    20641 tgatttttga ttgtttcttt tttatacgtt tttcttatgg aaaatggttg attccgttgt
    20701 ttgttttact tgcttgtttt acttgcttgt ttatcagttt cctttagcat caactgctgg
    20761 tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat agcgttctac
    20821 tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac aagtttgttt
    20881 ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat cgtatttggt
    20941 ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac tttataaatt
    21001 gaaactttca attaagaatg acagtttttt at
//
LOCUS       DAZUMM010000049        20975 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000049 DAZUMM010000000
VERSION     DAZUMM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.049"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..195)
                     /locus_tag="ACG8E6_001703"
     CDS             complement(<1..195)
                     /locus_tag="ACG8E6_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062115.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
     gene            complement(211..825)
                     /locus_tag="ACG8E6_001704"
     CDS             complement(211..825)
                     /locus_tag="ACG8E6_001704"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFS5062116.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(972..1655)
                     /locus_tag="ACG8E6_001705"
     CDS             complement(972..1655)
                     /locus_tag="ACG8E6_001705"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS5062117.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1764..2222)
                     /locus_tag="ACG8E6_001706"
     CDS             complement(1764..2222)
                     /locus_tag="ACG8E6_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFS5062118.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2306..4069)
                     /locus_tag="ACG8E6_001707"
     CDS             complement(2306..4069)
                     /locus_tag="ACG8E6_001707"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFS5062119.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4290..5750)
                     /locus_tag="ACG8E6_001708"
     CDS             complement(4290..5750)
                     /locus_tag="ACG8E6_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062120.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="ACG8E6_001709"
     CDS             complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="ACG8E6_001709"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFS5062121.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6907..8334)
                     /locus_tag="ACG8E6_001710"
     CDS             complement(6907..8334)
                     /locus_tag="ACG8E6_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5062122.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8365..9579)
                     /locus_tag="ACG8E6_001711"
     CDS             complement(8365..9579)
                     /locus_tag="ACG8E6_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFS5062123.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(9665..9892)
                     /locus_tag="ACG8E6_001712"
     CDS             complement(9665..9892)
                     /locus_tag="ACG8E6_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062124.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(9920..10162)
                     /locus_tag="ACG8E6_001713"
     CDS             complement(9920..10162)
                     /locus_tag="ACG8E6_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5062125.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10194..10751)
                     /locus_tag="ACG8E6_001714"
     CDS             complement(10194..10751)
                     /locus_tag="ACG8E6_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5062126.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10764..10952)
                     /locus_tag="ACG8E6_001715"
     CDS             complement(10764..10952)
                     /locus_tag="ACG8E6_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS5062127.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(10965..11531)
                     /gene="amaP"
                     /locus_tag="ACG8E6_001716"
     CDS             complement(10965..11531)
                     /gene="amaP"
                     /locus_tag="ACG8E6_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS5062128.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            11948..12121
                     /locus_tag="ACG8E6_001717"
     CDS             11948..12121
                     /locus_tag="ACG8E6_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062129.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            12761..13234
                     /locus_tag="ACG8E6_001718"
     CDS             12761..13234
                     /locus_tag="ACG8E6_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFS5062130.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            13243..13470
                     /locus_tag="ACG8E6_001719"
     CDS             13243..13470
                     /locus_tag="ACG8E6_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5062131.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            14106..14477
                     /locus_tag="ACG8E6_001720"
     CDS             14106..14477
                     /locus_tag="ACG8E6_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062132.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(14733..14975)
                     /locus_tag="ACG8E6_001721"
     CDS             complement(14733..14975)
                     /locus_tag="ACG8E6_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5062133.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(15007..15909)
                     /locus_tag="ACG8E6_001722"
     CDS             complement(15007..15909)
                     /locus_tag="ACG8E6_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5062134.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(15922..16110)
                     /locus_tag="ACG8E6_001723"
     CDS             complement(15922..16110)
                     /locus_tag="ACG8E6_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS5062135.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(16124..16687)
                     /gene="amaP"
                     /locus_tag="ACG8E6_001724"
     CDS             complement(16124..16687)
                     /gene="amaP"
                     /locus_tag="ACG8E6_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS5062136.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(16725..17618)
                     /locus_tag="ACG8E6_001725"
     CDS             complement(16725..17618)
                     /locus_tag="ACG8E6_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5062137.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(17696..18619)
                     /locus_tag="ACG8E6_001726"
     CDS             complement(17696..18619)
                     /locus_tag="ACG8E6_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS5062138.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(18668..19018)
                     /locus_tag="ACG8E6_001727"
     CDS             complement(18668..19018)
                     /locus_tag="ACG8E6_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS5062139.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(19051..19953)
                     /locus_tag="ACG8E6_001728"
     CDS             complement(19051..19953)
                     /locus_tag="ACG8E6_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS5062140.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(19946..20803)
                     /locus_tag="ACG8E6_001729"
     CDS             complement(19946..20803)
                     /locus_tag="ACG8E6_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS5062141.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
BASE COUNT         6033 a         4183 c         3439 g         7320 t
ORIGIN      
        1 gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac catctaaagt
       61 aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact gcccttgcag
      121 aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg atggtttata
      181 gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg tgaaaatttc
      241 tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt ttcttggatc
      301 gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct gtacttctag
      361 caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt catataagga
      421 tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta ctgaatcagt
      481 taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg ctgctgtttt
      541 atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc ttgtctcaag
      601 aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca aggataagat
      661 gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca ctatatcttt
      721 tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa tcagcaactt
      781 gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt cgccttcttt
      841 ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg cttttttcat
      901 cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag aatctgtgcc
      961 gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt agtgatcaat
     1021 tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc atatgctttc
     1081 tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc gacttgttct
     1141 atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata ttctgtatag
     1201 cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag cgcttcctca
     1261 aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg gacaagttca
     1321 gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat gatttctgct
     1381 tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac ttctgaccca
     1441 tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt gatttcattg
     1501 atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc agccaaagcc
     1561 attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg acaagaaacg
     1621 acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt aatgttatat
     1681 aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa taatgagcta
     1741 tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc gtatacgacc
     1801 agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt aaaaaactca
     1861 gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt ccaagcatgc
     1921 tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat gatacaaact
     1981 ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa aggctcattc
     2041 tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt tacaactagt
     2101 tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc taatttttct
     2161 gattgataac taggatattt ttcttggaac tttttgacat attcttctac ttgctgatac
     2221 ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta gtataaacaa
     2281 ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga ctatcttcaa
     2341 cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga acgctatccg
     2401 tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca taataacgac
     2461 tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg actaattcgt
     2521 gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt cctggattga
     2581 tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt cctaattcaa
     2641 gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt tgataggctg
     2701 gaatactata atcatttgcg atagctgtac catgttctac accatgtgta ggaattcgga
     2761 ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc atatcctctt
     2821 ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga atgtcaaatg
     2881 ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa ataatcactg
     2941 gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc aagaaagtac
     3001 ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg aaagtgatag
     3061 agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct tggaatgttt
     3121 tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct gtacttgttt
     3181 gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca gaagttactt
     3241 gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc attttattat
     3301 cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca atcggatgat
     3361 caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt ccattatcca
     3421 cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta ccgttggttt
     3481 gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata gacatctcat
     3541 tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc aatccttcaa
     3601 aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt cctgattctt
     3661 tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta gatgtgtgcc
     3721 ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc acttcacggt
     3781 aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca taagactgat
     3841 gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga aacgcaatcg
     3901 ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg tccttttgca
     3961 agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat aattttttga
     4021 catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata atacattctc
     4081 tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct attctactac
     4141 ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc ttgttatata
     4201 agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa ttaaaaagct
     4261 ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa taggtgaagt
     4321 tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg atagatcttt
     4381 gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac cactcttttt
     4441 ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat aggatttatt
     4501 tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa ttgtttgact
     4561 gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc actgttgatc
     4621 aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag ttggatttga
     4681 tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag ttgttctttt
     4741 tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca caacgtacgg
     4801 acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat ttcagcgata
     4861 ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa atttttataa
     4921 acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt tctactgttg
     4981 aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc tttttgctct
     5041 tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt cttatcttct
     5101 tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc tcgtatgata
     5161 aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac ataatcaatc
     5221 aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc ccaattgaca
     5281 taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat gatttcttta
     5341 tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat tgtatatact
     5401 ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat accattaaaa
     5461 taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt gagctcagaa
     5521 agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac ttgttctttt
     5581 atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt tggtgtagtc
     5641 acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa taattgttca
     5701 cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat tcctgataca
     5761 cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat ttttgtttcc
     5821 tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa
     5881 acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca
     5941 gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc
     6001 aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct
     6061 atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt
     6121 acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt
     6181 gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt
     6241 cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag
     6301 cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct
     6361 ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt
     6421 tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc
     6481 tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca
     6541 ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg
     6601 aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct
     6661 ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg
     6721 gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt
     6781 ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag
     6841 tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt
     6901 cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat
     6961 aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc
     7021 tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc
     7081 taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag
     7141 actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag
     7201 gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct
     7261 taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc
     7321 aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc
     7381 tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa
     7441 acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa
     7501 taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt
     7561 agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa
     7621 cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt
     7681 ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga
     7741 aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac
     7801 ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc
     7861 gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca
     7921 gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc
     7981 cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat
     8041 ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac
     8101 ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa
     8161 tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta agctgaagcc
     8221 tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg tcatagccaa
     8281 aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt gcatgaatct
     8341 tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa
     8401 tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa
     8461 aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa tcacttgaat
     8521 ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg caaagatccc
     8581 tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat caagtgcatc
     8641 atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac ccgcaatgat
     8701 cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa tggctgccaa
     8761 tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc
     8821 aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt
     8881 gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc agccaaacca
     8941 aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc tttctcctaa
     9001 aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt
     9061 gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa atccgccacc
     9121 ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa caagtataat
     9181 atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac
     9241 gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac catgaaacat
     9301 gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc caataacacc
     9361 gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa tcgttgttat
     9421 cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc gtgctaatcc
     9481 gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac ataaaatcat
     9541 gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct cctttctacg
     9601 attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga attaatactt
     9661 gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt tttcgttttt
     9721 cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt ttattttcgt
     9781 ctttttttat ttttaaagat attttttcat aaggaataaa gaatacttca tataaaatat
     9841 ataaaaaaga accgcagcga gtgtcgctac ggttctcaaa tttgttcttc atttcaacat
     9901 aagtttttct tcaatttgct tataatttac gtgcaaagaa tgaaacgact aatactaaaa
     9961 tgattgcacc gatcaatgat ggaatgatag ccattcctgc aattgttggt ccccaagttc
    10021 ctagtaaagc ttgtccaatt gctgaaccta ctaaaccagc aatgatgttt gcgatgatcc
    10081 ccattgatga tcctttattc gtgattgctc ctgcaattgc tccaatgata ccgccgacga
    10141 ttaatgccca aataaatccc attttaaatt cctcctaact gacttcttac ttcttattgt
    10201 acacgtggtt cgttattttc tttgactttt tgtgcacctt tgcctacagc agattttgct
    10261 ttatctgttt ggcgtgaagc aaattgtcct gtggattttg ctgcttctgt tactttgtct
    10321 tgtactgttt cttgatcttt ttggtattct tcttgtgttt tgatgtctgc aacattcaca
    10381 ttcacttcga taacatctaa atgtgtcata ttgtttactt cagtactgat caattctttg
    10441 atttggttgt aaatatcttc gatgtcttta ccgtattcta cgatcacatc tagatccaca
    10501 gctacttgtt tcttacctac ttctgtattg atacctgctg ttacgttatc tgtgttgact
    10561 aatttttcag ccatatttga gaaaaatccg ccatctaatg tcaatagacc atctacatct
    10621 ttcaatgcat aaccgatgat tttttggatg actttgtctt cataagtcaa ttcaccattc
    10681 atcatgcggt tttctccttt ttcatttgcc aaaactgatc catttgttgt attcatgttt
    10741 ttgttttcca tctttgtttc ctcctatgat ttaaaaaatt gatcaactat tcctgactgt
    10801 ttcaaataaa aaccgattcc tgcgccaatt gctgtcatga ttatgatcaa caatgtcttg
    10861 aaaaatccaa tcgttaacca taaaacagct agcagtagtc caagaccccc aaagataata
    10921 gggagcttgt atgctgaaaa tagttctttc attatttggc cctcctattc cactcgagag
    10981 tgcttgtagt ctcttgtatc ttcttgttct tcataaccgc tgtatgtgac tgccactttg
    11041 accgtttctt ttgttccgag tactttagtt acttcttctt cgatatcttg catcaataca
    11101 cctgttttcc ctactaatga agaagttcgt ttcaagtctc cgttgacatg gatatggatt
    11161 cgatttttcg ttgaacgtat ccgtacttta ggtgaatgaa tgaactcttc ttcgtgtaaa
    11221 tgggaacgta ccaacccttc gatcgctttt ttgtcgattg ttagtttgcc atcttcccgt
    11281 ttcataacga aagttcctct tgactttgga tagaaaagaa cgactagcaa gatgataata
    11341 gcaagaacgg caaatactac agctgcccag aaaatatatt gttgcatgta gtaattcgtt
    11401 aaagtaaaga agcgaaatcg ttccaattgt tcaggcataa tatacggcgc attgatcaac
    11461 gcaatcgcta gaaatactga aagtagcagt aaactcatga taacaccgat tatttttaac
    11521 cccttattca ttttccacct cctataaact tatttttacc ctgtaaataa ctctaccgac
    11581 aatcacagta tagatagcga acagttagca tagcatcagt aaataaagcg ctaccaaaca
    11641 ttgctggatc ataacggcgt tttattgcca gtctgatact gaaataacaa ttgcttactt
    11701 attctctatt tacttttgat tgctggctat acttaaagga tattattcaa gattaaaaat
    11761 tgcaaagatt atgctcagtt attattacaa gattctattt atttattaaa ttttttcgga
    11821 gaattaaaaa atacaataaa taactttagt tactttaatg cgttcttact ttatttttat
    11881 attatcattt ttcttattca cttctttggt ttatactatt cttataaata aaaacgaggt
    11941 atgtcctatg atgaaaagat tgtactattc attgatcata acgattggct atttgattgt
    12001 atcaaatcta ggaaatatgg tatttggtat cagtaaagaa ttcagttgga ctactacatt
    12061 atgggaatct ctattctttt tcatcttcgt atttttgtta caaaattatc gaaaaaaata
    12121 aacatgtgcc atctatctgt tggcacatgt tttaggattt atggtacttt ttattcggca
    12181 atccatttta tttttctact tcttcttaat acctctaaca cttttgattt ataaactaat
    12241 ccaatgcatc tacttgcgct tattaagcta tttcagctag atagtaccac cccatgcgtc
    12301 aatgcttgat tttccttttg tataacattg tataagcaat aaaggacaaa taagtggtga
    12361 ttacaccaaa ccattgaata agaaaaattc tgatacgtaa ataaacatga acttgaaaaa
    12421 attaatgaat cggttagcta ctacgtagtt gtcttttttc tcatacagtt ctgttaactt
    12481 acacttctct tcgctagata ataaaaaatc ctccaactgt attgtccaat ataatcccag
    12541 gcatctaaac tagcattata gaagagacac acatagaatc taaaataact ggttttaaat
    12601 gtttcttaca atcaaaaaaa gctgttaaat gcactccaca ccttcatact ctatcatttt
    12661 ttataaacga tatattttta ttgtttttcg ataaaaaata gttgttttac aataattatt
    12721 ctgctatatt ttagctatca aagataaaag agggataaaa atgaaattta aaacattgct
    12781 attaaaattt gtcgttttag gtataagtgc cgtatttatt ttgttcggcg tactttttta
    12841 tactcagatc gctacaagcg aaaaaggatt caattttgat tggcagacca cattatttgt
    12901 ttctgtcatt tactttgccc ttctcctagg gatcgttatt gcttatttct tattgaaact
    12961 attgaaaaaa atcgaagata accaagcttt ttccactcaa agcttaaaca tactaaaaaa
    13021 aatcgaacga accattttgt ttattggttt cgcctttttt ggcttacttc ctaaatttat
    13081 gaaagtagcg gatttagacg atgctccagg tttgatgttg gtaggaatcg ctgtcgtatt
    13141 cattcctttt gcagtataca cattaacaag cgtgctggaa aaactgcttg aacatgcgat
    13201 tttacttaag acagatcatg atttgacagt gtgaggtgcg acatggcaat tatcgttaat
    13261 ttagatgtag tattagctca aaaaaaaatg agtgtgactg aattgagtga gaaagtgggc
    13321 attacgatgg caaacatctc tattctaaaa aatggaaaag caaaagccat ccgtttttcc
    13381 actctcgaaa aaatctgcca agttttggag tgtcaaccag gtgatttgtt gacctataaa
    13441 aatgactcga atactatacc agaagaataa aataaagtag aacaaacagt aaataaaaaa
    13501 taatctgaat gatagatgtg tgagactttt agaagttatt tacgtctatt attcagattt
    13561 tttatttcga ttgaataccg ttttatctat aaataagata tgtactgact catacaaagg
    13621 gggccttttc ttctccatgt actatcactt ttcctgatac aactggtttc ctgttacgtt
    13681 gtagttggat ctgcttttag tataatactt tattcattcc accagactga tagcagtttg
    13741 agcagtctaa ataatcatgt agtattacag gccattctgt ttcatacctt gaagtgctta
    13801 aaacttcagc taatggccca ttgtgttaac tcttactcca ttctttttta gtaagctccc
    13861 atatatactg taaagccttc tatatattat gtcatgttgc tttttaacat ttactatgta
    13921 gttttgataa aatgatttga ataacagtga tggacaattc ttttttattt ttaacattat
    13981 tttgataata atgattattt atttaaaaat acattagata acatttgtta cgacacgaaa
    14041 aagcttcaca aacaaatatc gttattttaa aatgagggcg aactcacaca attaggagga
    14101 atagtatgcc atttattgtt tacttttttc tttcattact cgtgatctat attccattac
    14161 caacattttt cctgctcgtc aatcgtctag cagagcaaca tgatacgatt gctgctctta
    14221 cgaaactttg tttacttcta tttgtttgtc tagattactt tacgagctat tattggctaa
    14281 aacagacaag agtaaaaaag agatattatt tcttccttgt ctttttcaat ctctttgagt
    14341 ttctgatcca ttgcttttta cttcatcagt atcatacgat ccatttgtgg atcatctggg
    14401 ttttcaaagt ggtcgtatta ttttgtatgt tcacctttcc tatctcaaga aaatttaggg
    14461 aggcattgtt ttattaaaga agcttctctg tatcaataaa gacacattta aggaaaaact
    14521 tttaaaaatc taattgggaa tttgcccgca taaataagag gctgtgacaa aagtcttgtc
    14581 acagcctctt attccgtata aacagtgttc aaaagcttgc atcttcgata atatgcccaa
    14641 ccaaatgaaa aatatgagaa gctatttttg tttggctgtt cttattactt gatgaagaac
    14701 agctttttca cgacctcttt ttaaaagtct ttttacagtt ttcgagcaaa gaatgatacg
    14761 attagtacta aaatgatggc accaatcaat gatggcacga ttgccattcc tgcaatactt
    14821 ggtccccaag aacctaataa tgcttgacca agtgctgatc caactaaacc tgctacgaca
    14881 tttgcaataa tccccatcga agaaccttta ttcgtgattg ctcctgcaat tgctccgata
    14941 attcctccga cgataagtga ccaaataaat cccatgtttc gttcctccta atgttttact
    15001 tccttattac tgaacccgtg gttctgtatt ttcttttact ttttgtgcgc cgcttccagc
    15061 tgctgatttt actttatctg tttgattcga agcaaattca ccggtttttt tcgctgcgtc
    15121 agttactttg tcttgcaccg tttcttgatc tttttcaaat tcttcttttg ttttgatgtc
    15181 cactacgtta gcgttgactt cgatcacttc taaatgtgtc atatttttta cttctttgcc
    15241 gattacttct tgcatttctt caaagatttt ttcgatatct ttgccatatt ccacaatgac
    15301 atccatatca actgctactt gttttttacc gacttctgtt tcgatcccag ccgttacatt
    15361 atctgtattc acaagttttc ctgccacatt agagaagaaa ccaccgtttg cagataataa
    15421 accgtctact tgttcaattg cgattccgat gattttttgc acaactttat cttcataatt
    15481 cagctcaccg cgcattgaat cttcctgttc agaatcatgc tttccttggt tatttgtatg
    15541 atcagaacca agttttcctt gtccgtttgt gtggtcagaa ctaagttttt cttgtgcttt
    15601 cgcatggtca gaaggatgtt tttgctcttc cacaggttta gaaggatcga ttgcatcatg
    15661 ttgttctgga actttagatt ctggagttcc tggatgatga ggtgcatgct tttctgcatc
    15721 atgtttcttc tcttctactc cttgttgaac gttagggact actggatctg gatgtgcaga
    15781 tactggtgct gttttgttct tttcatcgtt cacaccattt gattggtttg gtgtcgttgg
    15841 gtctggatgt gcagacactg gtttttggtt gttcatttta ttgttattat ttttgttatt
    15901 gttattcatg gatgtttcct cctaagattt aaaatagtga tctaagattt tcgtttgttt
    15961 cagataaaaa ccgatagcga cgccgatcgc tgccatgatc aagatcaata aggtcttgaa
    16021 aaagcccacg gtgatccata aaacggctaa tatcaatcca agtaccccaa agatgatagg
    16081 gagcttgtat gtcgaaaata gttctttcat ttttggtccc cccctattct actcgtttgc
    16141 ggtcattgct tcgtttagct ggttcttcaa agcctgtgta tttcaccgat actttcactg
    16201 gctcttttgt tcccaatatg tgttgtactt cattttcgat ttctttcatc aatgttcctg
    16261 ttttaccaat gagtgctgaa gttcgtttca actccccttt tacattgacg gcgatcttgt
    16321 ttttcgttgc tttgacattt acttttggtg aatggatgaa ttcatcttca tgcaagtgag
    16381 aacgaaccat tccttcaatt gcttttttat ccaacgtcaa ctccccatca ccatgtttca
    16441 gtagaaagtt tccgattgtt tttggataaa acaggaaaac aagtaacgtg ataattgtca
    16501 aaatagcaaa tgcaacggca agccaaaata catattgttg cacgtatgaa ttagtgatgg
    16561 aaaagaatcg aaacttctct agctgaaaag gcaatggata ggttgcactt aatagtatcg
    16621 caaacaaaag cacagatagc aaaagtaatg ttccgatcac tccgatcgtt tttacacctt
    16681 tattcatttt cgcacctcct cataggggtg aaacgatcaa cttcttataa gttgatcgct
    16741 tctccaccag ttactccgta aatttgggct gtaacataac ttgctttatt cgatgctaag
    16801 aaaacataaa ctggtgctaa ttctcctggc tgacctgcac gttctagtaa actttgctga
    16861 ccaaattcag ggatcgaatc agaaggttgt ccttcgtcta gttgtaatgg tgtccagatt
    16921 ggaccaggtg ctacaccatt tacacggatt cctttaggtg caagctgttt tgctaaaccg
    16981 atcgtaaagt tagcgattga agcttttgtt gctgcgtaat ctaataaatg ttcgcttgga
    17041 ttgaatgctt gaacagaagt tgttgttacg attgctcctc ctgctgggat atgcggaaca
    17101 gcttctttca ctaaagcaaa catagcgatg atgtttatca taaatgtgtc tttgacttgc
    17161 tcaagtggta actcagcaat agactgacaa gaaatttgct gtcctgcgtt tagtaccatc
    17221 gtatctagcc cgccaagttc actcacggct ttatctatga tttctttagg tgcttgttcg
    17281 tcacgaagat caaaaggtag taagacaatc ttacgtcctg catctttcac gtattttgct
    17341 acttcctctg catctttttc ttctcctggg aagaattgaa gagcgatatc tgccccttct
    17401 ctagcaaatg caatcgctgc tgcacgaccg atccctgaat ctccaccagt gattagtaca
    17461 cggcggtttt ctagttgatt attgccttca taggattctt ctccacaatc aggaacagga
    17521 atcatattct gctgtaaagc tggtgtatcc tgatcttgtt taggaaattt ctccgtatgg
    17581 tacaagtttc gcgggtcgat taattttggt tctgtcattg ctgtattcct cctagttttt
    17641 aaattgtatt gccaaaacaa atttctttgt ttgatacctg ttttaaatag attctttaat
    17701 ttgtataatc tttccggtat aaatggtaac ctaaaactac tgctgctatc aaagagccaa
    17761 agactacgag tataaagcct agagcagaac ttttacccgg taatcctcct gtattcattc
    17821 cccaaatccc tgcgaacaac gccggaacgg aaatgattaa tgcagctgaa tctagatatt
    17881 tcatgatatg atttagatta ttgtccatca tcccgttgaa tagatcacca atactctcta
    17941 ataaatctcg atagatctcg atcatttttt ctgtttgtcg ctgtctcaaa tggacatcat
    18001 agagtagctg ttcatcgtct aaacgtttga tgaaagtttc agaattccat aattgatcca
    18061 ttgtttcccg ttgatctctt aaggtatgat cgatatatac gatgtctctt tcaagatctg
    18121 cctgtttata cagttcttca ttttccgttg ttttacgagc tgctttatcc aaagcgtcaa
    18181 ttcgcttttt catctctctt agttctttca cgtaatgggt gtaaatatct aataatgtat
    18241 acgtgatgat cttttcaaac gagtttaaat gatttccatg ctttgtcagc agccgatcca
    18301 caaattcggt ttttctatcg atacaagtaa tcaagagatt atcggaaagt acaaaagata
    18361 ttggcgttaa ttttttttcg atacgattat ctgactgatc tagattaatc actaccaaag
    18421 aaaatggatg ctctagtttc gtctcataaa ggcgctccat ccgagaaact tcttccggat
    18481 attcggttcc tacgaaaata tcatcaggaa gctcgaattc ttcgattgct tgctcaatct
    18541 cttcgggctt tgcactatca agtacgagcc aattatattt tttttcatct gtttctatta
    18601 cattcttttc ttcatccaca aagtagtatc tcaatgtata cctccttttt ggactttctc
    18661 gaaactatta gctgaagaag cagttcttta atttgatatt ataatcgagt actgcgattt
    18721 cattttctaa tgaaaagctg tcaagctgtt tgttgaagtc catgttcaat cgtttaccga
    18781 aataattttg ctctaattta gatgtataga tgtccaataa tggatgctcg attttagtcg
    18841 catattctcc aaattccgaa acaaaaggaa tgatttgaaa aaagctgcct tttgcaaaag
    18901 cacctttgtc ccttagaaat ggatcggaat cattgataag cgcgagtgcg atttttgtat
    18961 tttccccaac cttttcaatt aacgtctcta ctgcattttt agacattgta atttccattc
    19021 ctactccccc tcttcagttg ttgtcttcta ttatttaaca actacatctg tttttggttg
    19081 aacttttgca tattttggat atttagggaa tttagctaca caacggaaag ccgtcatccc
    19141 tgcatatttt tcgtaatatt tcttcgttgg cccttcaaaa tccttgctcc acggtttcac
    19201 atgtcctgaa aaatggataa tgtaaggatt gttgcgagtt tcttcgtatt cacgttcacc
    19261 tgctgctgtt ggatgttttt ttgcttttgc catgatatag ccttgtgcat tccaacgcgg
    19321 atgaagtggt aaccaacggt catgtaagat agcatttaat gcatcttggt catggaatcg
    19381 taatttttct ggattatgtt cgatgaaatc taatactttt tgcgtaatat tttcatctaa
    19441 ccattttttc acattgatca gcatcaaacc tgagttgaaa taacgcattg atttagctga
    19501 aattttcatt ttttccagtc gttgatggaa cccagcgtct tcaacagctg ctacgattga
    19561 atcaccgaag tcaagacgcc ataatttaga gatatcttgt aaagcgatca tgtcgcaatc
    19621 catatataag atacgttcaa cttctgtgcc gcggaacaat tccggaatcg caatacgata
    19681 ataagctgtt tctggaatac gatcgctgat taccacattt gtaaagaatt ctttattgat
    19741 tttcaagaat tccacatctg agttcattcg tgcgtttttc actgagaagc gtaaaagctg
    19801 tttactttca aaatcgatat catcgtcaat cacatagaac cgaacaaatt tagacgggtt
    19861 gcaattatct aatactgaga caaataatgc agctaaatgt ggtacgaatt ttgtgttaca
    19921 acttgcgact actgctattt cttttttatt cattgatctc gcctactttc gttagagtac
    19981 tgtgtgctaa tttttttaag tataaattgg tatacggatg atctttcaac gtattccaag
    20041 gtttatcatg tcctgtaaag tgaacgatcg aaggtttttc atttccttct ttatactgac
    20101 gttcatattt ttcatttggt gctggatgac gttcaaaaat cagcgaagtc tgcatgttcc
    20161 attttggatg aagttgttcc caatcttcat aaagtacagc attcaaagca tcttggtcgt
    20221 gatagatgat tcgatcgcca ttttcactga ggtaatggat agttttttct gtaatttctt
    20281 ttttattcca ttgatcaatg tcaatcacca tcactcctga attgaagtac aagtcatcgg
    20341 aatcgattcc taatctttcc aaagctttca cttggcctgg atcgataata gcgccgattg
    20401 tttttccatt taacggctca tcatatagtt ttacgatatc gtctagtact aacacatctg
    20461 aatctaagta aaggactttc ttataatcct ctttagctaa taagtttggt aaagaaattc
    20521 ttaagtaagc ggtagttgtg atatggtcac tgactaggaa gtcttcgtag atatcttttt
    20581 ccactgtcag gaattggata cttgcattac ttgaatattt atttaccgtt tcttccaatc
    20641 cttctttact atattctgat agaccatcgt caatcacata aaattttatg cgtcttgttt
    20701 tattgcaatt ttctaatgca gttgcgatca tcacgctcaa gtaaggagca taattttcgt
    20761 ctgaagcagt taccacagga actacgccgt atctcttctc catcaaaatc actccttttt
    20821 ctaatctttt tctaacctgt tctacatcta gaatattcct catttggttt taaagcaaaa
    20881 tatatgcttt gttttcattg aatattttaa aattttttta tcacaaaacg catcggtaag
    20941 cgctaactat tcgtttttca tggcttttta ttcaa
//
LOCUS       DAZUMM010000050        20923 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000050 DAZUMM010000000
VERSION     DAZUMM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.050"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..3398
                     /locus_tag="ACG8E6_001730"
     CDS             120..3398
                     /locus_tag="ACG8E6_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFS5062142.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3939..5846)
                     /locus_tag="ACG8E6_001731"
     CDS             complement(3939..5846)
                     /locus_tag="ACG8E6_001731"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFS5062143.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNEQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5976..6851
                     /locus_tag="ACG8E6_001732"
     CDS             5976..6851
                     /locus_tag="ACG8E6_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFS5062144.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     ALIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKEWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6931..7905
                     /locus_tag="ACG8E6_001733"
     CDS             6931..7905
                     /locus_tag="ACG8E6_001733"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS5062145.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8085..9230
                     /locus_tag="ACG8E6_001734"
     CDS             8085..9230
                     /locus_tag="ACG8E6_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS5062146.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSCQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLEQLVRAVKK"
     gene            9367..9711
                     /locus_tag="ACG8E6_001735"
     CDS             9367..9711
                     /locus_tag="ACG8E6_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFS5062147.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9982..11252
                     /gene="efmA"
                     /locus_tag="ACG8E6_001736"
                     /pseudo
     CDS             9982..11252
                     /gene="efmA"
                     /locus_tag="ACG8E6_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
     gene            complement(11405..12004)
                     /locus_tag="ACG8E6_001737"
     CDS             complement(11405..12004)
                     /locus_tag="ACG8E6_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5062148.1"
                     /translation="MNYFTSDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12408..13529
                     /gene="mnmA"
                     /locus_tag="ACG8E6_001738"
     CDS             12408..13529
                     /gene="mnmA"
                     /locus_tag="ACG8E6_001738"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFS5062149.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNHAEVVFD
                     EPVRAITPGQAVVFYDEMECLGGGLIDHAYQETKVLQYV"
     gene            13775..15196
                     /gene="arcD"
                     /locus_tag="ACG8E6_001739"
     CDS             13775..15196
                     /gene="arcD"
                     /locus_tag="ACG8E6_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFS5062150.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFAIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVAFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15210..16328
                     /locus_tag="ACG8E6_001740"
     CDS             15210..16328
                     /locus_tag="ACG8E6_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFS5062151.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SEFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILER
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16388..17680)
                     /locus_tag="ACG8E6_001741"
     CDS             complement(16388..17680)
                     /locus_tag="ACG8E6_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS5062152.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLAHQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNAAALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLMGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSISSGHSPSHRQ"
     gene            17830..18390
                     /locus_tag="ACG8E6_001742"
     CDS             17830..18390
                     /locus_tag="ACG8E6_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5062153.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLEGIHETNQIILQIFEAFQLTKEEKVHRSRELCSMIHGYLSLRFLG
                     YFTKESTIDPEDSYFWMINDFIATLP"
     gene            18519..18896
                     /locus_tag="ACG8E6_001743"
     CDS             18519..18896
                     /locus_tag="ACG8E6_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFS5062154.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEAEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESGKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18829..20901
                     /locus_tag="ACG8E6_001744"
     CDS             18829..20901
                     /locus_tag="ACG8E6_001744"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS5062155.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEEQKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKEMRINPDF
                     TIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKMLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         6784 a         3788 c         4462 g         5889 t
ORIGIN      
        1 gttatatttt ttctttttat aaaatacact tattataatt ttgtttttta ttgacagtaa
       61 aaataaatta ggtaatactt taagtaaacg ctttctaatt tgttacggga ggaatacact
      121 tgttaaaaaa acgtataaaa tacagtgcgc taatgatgaa tctccttatg ctcagtaccc
      181 cggttttaat ttctgtcgtt gaagttgctg ctaacgaaca acagtctgtg aacgaagaaa
      241 atgcacacgt gaacattgaa gggatgtcaa caatggagga aataccaaga actagtgaag
      301 atatggatac ggaagaagga aatgatccta tgatgaaaga atcagaaaat gaaaagacaa
      361 aaaagcaatc tgaagaagca aatgaagagt ggaaaggggt tgttttcgga gaaagcacaa
      421 gtgctagtag gtatgccatt gaaccattag aagacggcgt gcggctctct tccacgaata
      481 acggcggaaa attccagtca agcggatcag atggaatgac ctattattat caagcaatcc
      541 ccaaagacat caactttagg atgcgggcga cgatcgaaat agaatcttgg acctatacga
      601 atgcccaaga aggatttgct ttaatggtaa gagacgcagt accaaaaaat catttgtttg
      661 ggcaggcatt ttattcaaat tcatttgcca tactaggcag tcggatcgaa tatgtctggg
      721 actcggatag acaagaagtg gtcaacacga gcggcagcaa atatacgatg cgacttggat
      781 tggggacacg ggcaatcaca ggaatatcat ctgaagatcc gcaagctgcg ccagctcctg
      841 ggagtgtctc agtggaaaca acacctttag agacttcagc agggttcttg gaaaaagaaa
      901 caggttctta taatatattc ggaaacggtc atgggaatct gtttccggcc acgaactcga
      961 ttactaaact ggacgtagag atgaaaaaaa cgaatactgg tttagaggca tattattacg
     1021 atcctgaaac aggagaagaa atggcttctg acatcatgta tgactgggaa aaattatacg
     1081 cttcagatga atcggttatt tatgcaggat ttgctgcagc aaggaatatg acgatcaagg
     1141 tagaaggaat cgaagttgct tactcagatc ctgcgacaga tcctcctggg caagaaagac
     1201 ctactcgctt gattgatccg atttacactg tgacctctag taatcattca ggaaaccaag
     1261 atcatactat gtcctttaga gcaaatgctg acggattatt gacaattaaa aataaagcaa
     1321 caggagaggt gcttaagaac gcaaaaaact tggctatcac tgcgaatcgg gaatttgatt
     1381 atcagacaaa actgatggaa ggaacgaacg aatttacttt cagttttacg ccagataaaa
     1441 actatccgcc agaagaatac gtagagatga ctagttatga aacaaaagag attcttcata
     1501 cagtggacta ccgtaaacct aaatattcga cagtttatgt aacaccagaa gggtcagatg
     1561 caggaaacgg aagcagacaa aatccattat ctctgacaca agcattgaga tacgcatatg
     1621 ctgggcagac aatcgtcatg gcgcctggaa cttattcctt cgaaagagga ttgacgattc
     1681 caaaaggcgt taacgggaca agagaaaacc cgattcagtt gattgcagag aaaaatccag
     1741 aaaacaagcg tcctgtactt gattttaaag gaactggaaa tgggatgaca ctgttcagtc
     1801 actactgggg tttacgaggg tttgacatta caaatagtgc agctatgcaa aaaggattac
     1861 agatttctgg aaatcataat ttgatcgaag agattcatgc ttatcggaat ggaaatacag
     1921 gaatccaaat cagcggtagc tcaaatgatc ctttcgaagc atggcccgcg cataacttag
     1981 tcaagaactg tacatctttt gaaaatgccg atccaggatt tgaagatgcc gatggatttg
     2041 cggccaaact gacaatagga gaaggaaatg tatttgacgg ttgtatcgcg tatcataatg
     2101 cagatgacgg ttgggacctt tttgctaaaa atgaaacagg ttcgatcgga aaagtaatca
     2161 tcaaaaactc agttgcttat cgaaacggct gggtaccagg aattgaggga gaaggtaacg
     2221 gtaatggatt caaaatgggc ggttcttctt taacgggccc tcatgaattg atcaatagtt
     2281 tgtctttcga aaatcttgcg aaaggaatcg attccaatag cggaacagat attatcgtca
     2341 atagcagtac aagttttaat aacggttcat ataatgtggc tctttatacc tcttcagcag
     2401 gaaacacaga ctaccatgct tctggtatct tatctttccg aacagagaat cttgagatga
     2461 gagagcagat ccgcccattg aatcaaaacg aagatcaaat ctaccactcg ttgaactact
     2521 attggaatga acgagagaaa caatcagaaa atactcttgg acataccata tcaaaagatt
     2581 ggatcgagtc tctaaataca tgttcgaaag aaggtcagca gcctgttacg cgctatgaaa
     2641 atggaagtat ccatgttcat ggactgatgc agattaaacc ggggaatcgt caaacagaaa
     2701 aggaaagagt ggatggtctt ccattatctg taggtgctat ttttgacgga gaaacctcac
     2761 cgtcttcgga atatccagag tatgcaatcg tacaagaacc aaatcagaca aaaccaaatg
     2821 aacaaaataa tccgattatt atacaagaag caaatggtgt ggcggagaat gcagaaggaa
     2881 atatcattga actgaagcct tttacagttt ttgtgtcaac tttcacacca aatcaaggaa
     2941 cgcctgtctt tgaagatatg cgttatttcc cagaaaagat ccaaatcgtt gaaacacagt
     3001 caagtgctca attttctaat cataccgcta ttttttcagc accttcaaaa ggagaatata
     3061 cgctagaagt cacttatcgt tcggaaatat ttgatgggga aaaatggtct gctcatacag
     3121 aatctgttac ccagcaaaaa gcgatcactg tgcgagaaac tgccgatgtg cccccgggaa
     3181 atgacaaacc agaaagagaa aatccaggaa atgaatcacc aaatggaaaa ccaaatggaa
     3241 aaccttctca tccggatagg acagcaaaag aaagtgaaaa aaatggcact caaggactcc
     3301 caaaaacggg agaatttaat aacccgttgc tagcattagc tggaggaatt ttgttaatcg
     3361 gtgtagttgt ttatgtcatg aaacagcgaa aaaaataagt ggaatttctc cttttatgat
     3421 gtaaaacaat tgactgagga aagtaaaaat agaaggaaat gagaccaata gatgtctcac
     3481 ttccttctat ctttttatag atgaggttct ccttgctgct tttgattggc tgttcttatt
     3541 acttgatgca ggacagcttt ttcacaatct ctccactaaa cggtgttcaa tcagctgacc
     3601 ctcggtatta gctcaacaaa cggcaaaata tgaggaacta tttttccgtt tttttctcta
     3661 atactcaggt caggacgctg atttcacaac ctctcgataa acggtgttca aaagccagtt
     3721 tctcggtagt aagtcaaatt tatgcaaaaa tgtggaaagc cattttcaca aaatttttct
     3781 tactacttga aactaagcag ctttttcaca acctctaata catggtattc cagaagtaac
     3841 ttcttcgaaa aataagccga aatgtcacaa aaatttgaga agcgattttc ccaaattcct
     3901 tcttatttct tgaagctgac cacttctggc acaacctctt accagattcg cactcttttt
     3961 tctggcgcta gatacatagc gtcttgttcc gtgataccaa atgtttcata gaattcgtca
     4021 aggttttttg gttgcacgtt tgctcggagt ttagcagggg catggacatc gatttgcagt
     4081 aaaagttgtt ggtattcttt ttttgctttt gtacgccaaa tcgttgccca attgacaaag
     4141 aattcctcga tagaaagatc tgattccgtt tttgctgctt ccaatgcaca gcttagaccg
     4201 cctgcatcag caatattttc cgaaacggtt aattttccgt ttacttttcc gtcagcaaaa
     4261 ggcagaccat cgaattcttc gatcattgct tgtgctttgt tttgaaattg tgtaagatct
     4321 tcttctgtcc accagttgtt caagttgcca tattcgtcaa ataaagcgcc gttattatca
     4381 aatgcatggg aaatctcatg agcgataaca gctccgattc caccataatt agcagagctt
     4441 gattgttcta gactgtaaaa tggtgcttga aggatcgctg ccggaaagac gatcacattc
     4501 cgaaatggat gataataagc attgaccgtg tcagcactca tttcccattc tgaacgatcc
     4561 actggtttgt tccatttgct gaaacgatct tttaatgtga gtttgctgaa aacaagagta
     4621 tttgaaagca aagtaccacc ctgatctgca ggtactgttt tgaactggtc gaataatgct
     4681 gggatcttat cggggtagcc tacttggata cctaagttat ttaattttat gacggctttt
     4741 tcttttgttg catccccaag ccagtcattt tcttctagac gttttttata aacggcgatc
     4801 atttttttca ccatttgttc tacgtctttt ttagcagctt cgccaaaata tttttttccg
     4861 tagtaatcgc caactacttg atcgaattgt ccagaggcaa ggtaatacgc tgcttttttc
     4921 tgaggcattg cttcgtctgt tccagacaat acgcgagaat agattccgct gacttggcgg
     4981 aattcttctg atagataacc actaagagaa cgtactgttt taatgatcat ccagcttttg
     5041 aataacggaa aatgagcttc tgtcaggatg tcattcaaat gatcgaagta cactggatca
     5101 gttacgatca ctttatctgg agttgtccca atcaaacccg tgacaagttt tttcaagtcc
     5161 agtttatctg tgtatttaat aaatgtatca aaagattgcg gattatacat tttgctgtaa
     5221 tctgcgtttt cttctgcact ttttacatga ggagcaatca atttatcaaa ttgcactgct
     5281 tgttccgtga ttgcttctgc ctgacttttt tctttgccgg caagctctaa taactgaaca
     5341 gccatatcaa agaaaacgcc tattagttgt gcaccgttgg gatggtctga tgcataatac
     5401 gtcttatctg gaaggaataa agaaggcgga taagcaaaga aggcgttcgt ttgcgcattc
     5461 ttcatatctg catctacatc cagattaaat ggcaatggta atgtatccaa cagccattct
     5521 ggaaattgct cattcaattc gtcgaaagac tgcaatgatt caactttttc gattataggt
     5581 tgaagtggtt gagcatgcaa ctcatttctt cggtcataat cgtttgccag ttgataatat
     5641 gcaaggaaat gtttcatttt ttctgaagac actttttctg gatgggcaag cagatcgtct
     5701 gtttctccca tcaaaagttg gtcgatcccg tcaactaaat cttggaaacc gcctgtagct
     5761 ggtttatctg cagggatttt tgctgttttt tgccattctt cattgactgc ttcatagaaa
     5821 tcttgtttca atcgttcttt gttcattcat cgatcgctcc ttttttgttt cgtgtgccca
     5881 ttatcttaca aaatgaagga attaacaaat agaacataaa aaattgaaaa ttcgaacaat
     5941 caaactgatt tgtttttttc aatccttcac ttataatgaa aaaaaggagg cgatcccaac
     6001 tgaatcaagt agtggataaa cctttatatt tcaaagtaga taaaaaaatt aaaaaattag
     6061 ccagcactca gcagctgcag tcaagaaaaa gtaaattgct ttttttagct ctggtttttg
     6121 aagatcaaag ttatgtgatt atcgatcaat caggacatcc ggtagaatat tctccagcaa
     6181 aatatactta tcaagagggt ctttcttgta aaaaatggaa gctgatcaat gaagagccaa
     6241 tcgagttatc tagatggatc aacagaaaag aagatattcc cgctttgatc gaagaaaaaa
     6301 ggtcaggaaa ggaactcgca aattgctggg tgggattgcc agaagaacgt tttttgaggt
     6361 ataagaaatg ggctaccccc tcaggctatc tttgtggtac ttacgcggca gcagttctcc
     6421 ttgcctatta tcaagactat cgtaaggagt ggatgcttcc aaatgagatt cgcaagaaaa
     6481 atacggctga ttcgttggtt ttgacgaagg cattgcgtag ccagatccaa ccactaggac
     6541 ttcctacgat tccttttcag gttagtacag ggatttctag ttttttaaag aagaatggca
     6601 atcatgagcg tgcacgagca actcttctag gttcatggca gcgagctaca aaacgtatac
     6661 gtgaaggcaa acctgtgatg attggtattt tgaaagtact tggcagtaca tatggcaatc
     6721 attgggtgac agcgtacgct tattttgaaa ctgaaacagg agaacgttat tacaaagtac
     6781 acgacaactg gggcgattat cataaagtca ttcctgccag ttggagtaac ggaacagttt
     6841 cacttcctta agaatttgct gaaattaaca gaaaatcttg gaaaaatatg ttaaactatc
     6901 gaagaataga gaagttttgg ggaggactac atgagtaata agtatcagga tgacacgttg
     6961 aagattttta gtttgaatgg caaccgtcca ttagctgaaa aaatcgcgaa agtgttcgga
     7021 acagaattag gtaaaagcgt cgtgaagcaa tttagtgatg gcgagatttc catcaatatc
     7081 gaagaaagta ttcgagggga tcatgtgtat atcgttcaat caacgaatgc acctgtcaat
     7141 gactactata tggaactact gatcatgatc gatgcaatga agcgagcaag tgcgaaaacg
     7201 atcaatgtcg tattgcctta ctatgggtat gctaggcaag accgcacggc gaaacctcat
     7261 gaaccgatca ctgcgaaatt gatcgctaac ttgatcgaag aagcaggggc aactcgagta
     7321 ctgactttag atctgcacac ggttcaagta caaggattct ttgatattcc tgtagataat
     7381 ttatttacga tgccgttgtt tgctcattat taccgtcaat taggtctgac gggagatgac
     7441 atcgtagttg tttcgccaaa aaacagcggt gttcagcgtg cacgtagtct ttcagaatat
     7501 ttgaacagca cattagccat cgtagatcat gcagatgatg cttcaactga gggtggttat
     7561 gtgatcggtg atgttcaagg aaagacatgt atcatggtag atgatatctt gaacacagga
     7621 gcaacgcttg cacgagcagc aaatgtatta aaagaaaacg gcgcaaaaga agtatatgcc
     7681 tgtgcgtcac atggattatt gtccgatcat gcgaaaagta ttttagacaa tgcaccaatc
     7741 aaagatattt gtatcactga ttctgttttc acagaagaaa atcgtcatcc agaaaactta
     7801 acaatcgtta catgttcaga attaatggga gaagcggtca aacgcatcca tgaaaataca
     7861 ccgatgagtc cgttattccg tctggaagaa aaagaattcg aataaacaaa aaaaagaggg
     7921 aaagaagttg tgacacaagt gagcagcttc aaggagctgc gacaagactt ttgtcatagc
     7981 tccttttttc tatgcccatg aaacgtcctc tagacaaaaa tgagctcttc tttggtttgt
     8041 tcgctatttt atgctagaat gtgcctagca ataatttgga agggatgaat gaagtgaaaa
     8101 ctatttatct ggatcatgcg gcaactaccc ccatgcatcc acaagtaatt gaagcgatga
     8161 cggcaatcat gcaagacacg tttggaaatc cttcaagcat acatggattt ggtcgccacg
     8221 cgcatgaaaa attggaaaca gcaagacaaa tcattgcaga cagtctgcaa gcaaaacctc
     8281 acgagatcat ctttaacagt gggggaacag aaggggataa tacagctatc ttagaaacag
     8341 cgttctcgtg tcaaaaagaa ggcagacatc taatcactac agctatcgaa catcctgctg
     8401 ttcttaatac catgaaatat ttagagacaa aggggtttga cgttacatat ctgcctgtag
     8461 atgaaaaagg gaatctgtcg atcgaagagg tcaaaaaagc gttacgagaa gatacgatct
     8521 tggtctctgt gatgtatggg aacaatgaga tcggaaattt gatgccaatc aaagaaatcg
     8581 gtgaactttt agcggatcat ccagcttatt tccatacaga tgcggtacaa gcgtacggaa
     8641 accaaacgat ccatccagaa gaattaggaa tcgatttatt aagtatctct gctcataaaa
     8701 tcaatgggcc aaaaggcgtc ggtttcttat ataaacgaga cggagtaaat atccctgcat
     8761 tgcttttagg cggggagcag gaagaaaagc ggcgtgcagg tacagaaaat ttagcaggga
     8821 tctggggtat ggcaaaagca gttgaactgc tgtcacctga agaacgtgaa gcacgtaaca
     8881 acaaatataa aggattccag caattgattc ttgatacatt agaagcaaac gaaatccctt
     8941 atcaagtaaa tggtgattta acgaataaac tccctcatgt gttgaatctt cgcttaattg
     9001 gcgaagcaaa tgatttgcta ctgatgaagc ttgatcttaa tggggtcgct atttctactg
     9061 gttcagcttg tacagcaggt aatatcgagc cttcacacgt attagaagca atgtatggag
     9121 aagatacacc gatactaaaa gaatccatcc gtatcagctt tggtttggga aataccgaag
     9181 acgacatcca gtatttcctt gaacaattag ttcgtgctgt caaaaaataa ctagtgaagt
     9241 cattcttata tgaacagatg tctgagagaa cgccttcatt ttttagggaa cttaactgaa
     9301 aaacctagta aaaatcagga taaactttta taataaagag aaactaattt tatcgaggtg
     9361 aaaatcatgg catttgaaac aacagcaacg gtactgggcg caccaattag ctatcgtttg
     9421 catcctgatg caaaaagata tacattgcgc gataatggat ttacagaaac aaacggaggc
     9481 aattatcaat tgatccgacc attagatgcc acgccgcaaa gcaaagaagg attcaaatta
     9541 aaaatcactg tatcaaaaga tattcaaaca ttgaaaatgt cgatcactac agcaaatgga
     9601 ttgaaagcag tcaatatttt caaagatcca aaacagaaaa tgagccaaga taaattttat
     9661 tttctgatgg atgggatgat tagccgtggg ttattcgaaa aagtcgaatg atctgtggat
     9721 ataaaaagac aactagtttt gaaacggcct attgttagat gactaacttt gtaaggacaa
     9781 cgcaaattag ttgtcttttt tatttttaaa tttaacacat gttgttattt ttaatatttt
     9841 gctgatttat tgtttctaag taaagaaatt taaattgaaa cgattgttat ttagggaaaa
     9901 aggtattttt tgaaaaaaga atcccgtata atgattgttg tgcaaacgct atacaaaatg
     9961 aaaaagaaaa gagggatgtt tatggaaaat gaacagtcgg tcgttttaac gaattggaag
    10021 agaaattatt tgttcttttt atcgggacag tttttgtcag gaatcactag tatggtcgtc
    10081 caatatgcga tcatctggta tttgacaaga gaaaccggtt cggcgacaat tttgagtttt
    10141 gctactttac taggaatgat tcccatggtt ttactaagcc catttgtaga accgctagtg
    10201 gatcgctggg ataaaaaagc tcttttgatt gtcacggata ttattgtagc gattttcgcg
    10261 cttattttag cagtagtcgg aacgatttca gaatccttcc cgatttggct agtttttgtg
    10321 tctttgttta tgcgttcggt cgcacaaaca ttccaaatgc caacgatcag tcgattatgc
    10381 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
    10441 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
    10501 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
    10561 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
    10621 caaaatttgg gctgcagcaa ttgatggata acaaagggtt atggtacata acgatcaatg
    10681 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
    10741 gttactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
    10801 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
    10861 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg ggtccaaacg
    10921 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
    10981 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
    11041 gtgtcttagg gaacttcaac tctttgatga acctggcagg tccaattgga ttattatttg
    11101 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttct ggaatcggga
    11161 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc tcgaaattat gataaagaat
    11221 tacagaaaaa attggtaaaa gaacaccatt agtaaaaaga cgaatagtat ggaatggagt
    11281 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
    11341 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
    11401 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
    11461 ataacggagt gccaaatggt agtttttcct ttaagagttc cctttccggt gcgtctacgc
    11521 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
    11581 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
    11641 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
    11701 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
    11761 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
    11821 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
    11881 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
    11941 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg gtaaagtaat
    12001 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
    12061 cataacatct aaaaagaaaa ggttgtaaat gagacgtgta gctttaagcg ataagaagca
    12121 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
    12181 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
    12241 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
    12301 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
    12361 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
    12421 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
    12481 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
    12541 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
    12601 aaatcgggat tccttattat tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
    12661 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
    12721 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
    12781 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
    12841 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
    12901 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
    12961 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgtttt attggtgaaa
    13021 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
    13081 ttgatggtga agtcaaaggg caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
    13141 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
    13201 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
    13261 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
    13321 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
    13381 atgataatca tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
    13441 cagttgtatt ttatgatgaa atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
    13501 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
    13561 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
    13621 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
    13681 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaaggaaatc ttttgcaagt
    13741 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcaa cgggtattag
    13801 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
    13861 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
    13921 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa aacgtccaga
    13981 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
    14041 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
    14101 gatgagcgcc atgggagaat ttttcgcgat attcaaaggt ggacaaaatg ttccttctat
    14161 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
    14221 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
    14281 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
    14341 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
    14401 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
    14461 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
    14521 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
    14581 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
    14641 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
    14701 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
    14761 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
    14821 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
    14881 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
    14941 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagctt ttgaattgat
    15001 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
    15061 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
    15121 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
    15181 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
    15241 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
    15301 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtgaattt ttcgaaaaac
    15361 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
    15421 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
    15481 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
    15541 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
    15601 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
    15661 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
    15721 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
    15781 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
    15841 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
    15901 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
    15961 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
    16021 aaaaaatatt ggagcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
    16081 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
    16141 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
    16201 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
    16261 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
    16321 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
    16381 tttacgctta ttgtctatga gaaggagagt gccctgaaga tatggagact cctgaagctt
    16441 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
    16501 tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactagaaa
    16561 cactggagcc gatcataggt cctactacgt taccagttgc ttggaaagat tggttataac
    16621 tgaagatccg tccagcagct tgatgaggag aatattttgt gatcaatgct tggacagcag
    16681 ggagcagaca agcatcagag atacccatca aaaaacgtaa ggcagctaat tgccaaacat
    16741 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
    16801 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
    16861 caatcccagg tatcgaagcg atgattccgc tgatcaacgt aacattccca tgtccatgca
    16921 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
    16981 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
    17041 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
    17101 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
    17161 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
    17221 cagttgctaa agttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
    17281 gggcagcggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
    17341 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
    17401 cacgcaacag catcaatttt cttccttttt ggtgggctaa ttttccccac caaggagaga
    17461 tcagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
    17521 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
    17581 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
    17641 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
    17701 aattttcatc tacagctttt tgttataaac tacaatatgt agtttatcaa tgaaagtctc
    17761 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
    17821 aacatgaaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
    17881 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagcaaa acgcttggat
    17941 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    18001 acagcgaaac ttctgcagga acttaatgac cagctgcgtc gggctcttgt gggaaaaagt
    18061 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    18121 gcagtgtatg agttactgat cagtgtgccc cacaccaatg aagcagtttt attagaggga
    18181 atccatgaaa cgaaccagat cattttgcag atctttgagg cttttcaatt aacgaaagaa
    18241 gaaaaagtcc accgaagcag agaattatgt agtatgatcc acggctatct ttccttgcgt
    18301 tttttaggat actttacaaa agaatcgaca atcgatccag aggacagcta tttttggatg
    18361 atcaatgact ttatcgctac tttgccttaa acaatcagat tcatttatat cattttcaag
    18421 atgttcatta aatgatcctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    18481 aagataagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    18541 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagcagaag
    18601 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    18661 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    18721 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaaa ggacttgcgt
    18781 tgttgaaaaa tcatcagccc atcacagtag aaagcggaaa ggaattttat gaaacaacta
    18841 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    18901 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    18961 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    19021 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    19081 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    19141 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    19201 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    19261 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    19321 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    19381 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    19441 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    19501 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    19561 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    19621 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    19681 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    19741 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    19801 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    19861 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    19921 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagca aaaaatctat
    19981 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    20041 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    20101 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaagaaatg
    20161 agaatcaatc ccgatttcac catcaaaaaa gaactatctg caccgcctaa accattgtca
    20221 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    20281 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    20341 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    20401 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    20461 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    20521 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    20581 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    20641 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    20701 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    20761 gaaaaaatgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    20821 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    20881 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tga
//
LOCUS       DAZUMM010000051        20645 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000051 DAZUMM010000000
VERSION     DAZUMM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.051"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            282..971
                     /locus_tag="ACG8E6_001745"
     CDS             282..971
                     /locus_tag="ACG8E6_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5062156.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            958..2166
                     /locus_tag="ACG8E6_001746"
     CDS             958..2166
                     /locus_tag="ACG8E6_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS5062157.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2460..3731
                     /gene="serS"
                     /locus_tag="ACG8E6_001747"
     CDS             2460..3731
                     /gene="serS"
                     /locus_tag="ACG8E6_001747"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFS5062158.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4280..5764)
                     /gene="guaB"
                     /locus_tag="ACG8E6_001748"
     CDS             complement(4280..5764)
                     /gene="guaB"
                     /locus_tag="ACG8E6_001748"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFS5062159.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6269..6964)
                     /locus_tag="ACG8E6_001749"
     CDS             complement(6269..6964)
                     /locus_tag="ACG8E6_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFS5062160.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7054..8154)
                     /gene="ychF"
                     /locus_tag="ACG8E6_001750"
     CDS             complement(7054..8154)
                     /gene="ychF"
                     /locus_tag="ACG8E6_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFS5062161.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8163..8354)
                     /locus_tag="ACG8E6_001751"
     CDS             complement(8163..8354)
                     /locus_tag="ACG8E6_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFS5062162.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8375..9265)
                     /locus_tag="ACG8E6_001752"
     CDS             complement(8375..9265)
                     /locus_tag="ACG8E6_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFS5062163.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9252..10019)
                     /locus_tag="ACG8E6_001753"
     CDS             complement(9252..10019)
                     /locus_tag="ACG8E6_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS5062164.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10031..10744)
                     /gene="rsmG"
                     /locus_tag="ACG8E6_001754"
     CDS             complement(10031..10744)
                     /gene="rsmG"
                     /locus_tag="ACG8E6_001754"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFS5062165.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(10976..12877)
                     /gene="mnmG"
                     /locus_tag="ACG8E6_001755"
     CDS             complement(10976..12877)
                     /gene="mnmG"
                     /locus_tag="ACG8E6_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFS5062166.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12894..14291)
                     /gene="mnmE"
                     /locus_tag="ACG8E6_001756"
     CDS             complement(12894..14291)
                     /gene="mnmE"
                     /locus_tag="ACG8E6_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFS5062167.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14590..14973
                     /locus_tag="ACG8E6_001757"
     CDS             14590..14973
                     /locus_tag="ACG8E6_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062168.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15141..15899)
                     /gene="jag"
                     /locus_tag="ACG8E6_001758"
     CDS             complement(15141..15899)
                     /gene="jag"
                     /locus_tag="ACG8E6_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFS5062169.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15917..16735)
                     /locus_tag="ACG8E6_001759"
     CDS             complement(15917..16735)
                     /locus_tag="ACG8E6_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFS5062170.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16732..17088)
                     /gene="rnpA"
                     /locus_tag="ACG8E6_001760"
     CDS             complement(16732..17088)
                     /gene="rnpA"
                     /locus_tag="ACG8E6_001760"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS5062171.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17206..17340)
                     /gene="rpmH"
                     /locus_tag="ACG8E6_001761"
     CDS             complement(17206..17340)
                     /gene="rpmH"
                     /locus_tag="ACG8E6_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFS5062172.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17863..19197
                     /gene="dnaA"
                     /locus_tag="ACG8E6_001762"
     CDS             17863..19197
                     /gene="dnaA"
                     /locus_tag="ACG8E6_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFS5062173.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19395..20525
                     /gene="dnaN"
                     /locus_tag="ACG8E6_001763"
     CDS             19395..20525
                     /gene="dnaN"
                     /locus_tag="ACG8E6_001763"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFS5062174.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5908 a         4215 c         3794 g         6728 t
ORIGIN      
        1 tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac atttctagct
       61 tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc cgagagacgt
      121 aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac caactagatg
      181 tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct gctttttttt
      241 gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt ttgattgctg
      301 acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag gggtatgaag
      361 cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc cctgatattg
      421 aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta gtgaaagagc
      481 tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact gatatggata
      541 agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt aatccgttag
      601 aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt gccccagatc
      661 agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat gaagttaaaa
      721 caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc tacttgcttg
      781 ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt tggaaacaag
      841 aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga gataagattg
      901 aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc tataaaattg
      961 acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac ttcaaaagaa
     1021 atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct attaaatgtt
     1081 tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac gaatgcgatt
     1141 tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg gaacggccgg
     1201 aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg gcgcttgatt
     1261 cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta catcgcaaac
     1321 ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg agtggtaaca
     1381 agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg taaaatcgaa
     1441 aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc actcacgtcc
     1501 atcattggat acctaggact gatagaagat ggccagtatc actcagaaga agatctttta
     1561 aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt ggacgactta
     1621 tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc ctttgacatg
     1681 atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa aaaaaatatc
     1741 caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac cgaaaaactt
     1801 gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg agcaacgaag
     1861 atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa aaataacgga
     1921 gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg tgtagaagaa
     1981 tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca aagcattgtg
     2041 gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc tttcatcatg
     2101 caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt tattgacgaa
     2161 agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac acagaaccca
     2221 gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa ggataatttg
     2281 gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca acagccgtta
     2341 tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt cagactgatt
     2401 cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga ggaaatcata
     2461 tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa ttgatgactc
     2521 ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat cgtcgtaagc
     2581 tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct gagatcgccc
     2641 aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa gaagtcggtg
     2701 gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg aaacaaattg
     2761 caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat gaagaggaca
     2821 atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca aaaccacact
     2881 gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa gtagccggta
     2941 gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc tacaacttca
     3001 tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct tatatcgtca
     3061 acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat gttttccaat
     3121 tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact aactattata
     3181 atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta agccctgctt
     3241 ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt cttcaccaat
     3301 tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa gaattggaaa
     3361 aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat cgtgtgatgg
     3421 ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta gaagtatgga
     3481 tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa gatttccaag
     3541 cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat actcatactc
     3601 tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa aactaccaaa
     3661 atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg aatctgaaag
     3721 ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga aaaattttgt
     3781 gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac tggcttttga
     3841 acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag agaaaaaaat
     3901 ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt cagctgattg
     3961 aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac aaggacaaat
     4021 tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag gtggcaaggg
     4081 aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga aataagaaga
     4141 aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg aaggagttgc
     4201 ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt
     4261 cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct tttgtgattt
     4321 gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata aattgcgctt
     4381 catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa cgcaaaccgc
     4441 cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga ccttcgattc
     4501 cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg tcttttgaac
     4561 ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga cgtccttgga
     4621 agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct agcatcactg
     4681 catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc cctccgtctg
     4741 caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata gctgtgagct
     4801 gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt ccgataccaa
     4861 cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc gtcgcaacgt
     4921 ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt tttcggatca
     4981 cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct gcttctaata
     5041 aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct acaagcaatc
     5101 gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg tctttgatcg
     5161 tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg attttatgtt
     5221 tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc actaagttgt
     5281 cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc atgtcgcggt
     5341 ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc acaccactga
     5401 tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc gttaagaaga
     5461 acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca tccgcttgct
     5521 ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc atagcaatgg
     5581 ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata ttcaacttaa
     5641 tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga ctttctgctg
     5701 gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt tcccagttgg
     5761 acatttttag taccactcct ctaatttagt ttatctatta aaataattga ttttttcatt
     5821 taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa tataaagcgt
     5881 ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa gtaataagaa
     5941 cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta ccgcagatgc
     6001 aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt tcgagtagta
     6061 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt aataccgaga
     6121 aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt ttgtcacagt
     6181 cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc cctagatatc
     6241 tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa cttccaataa
     6301 tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg ataatgataa
     6361 ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc gcagaaaaca
     6421 gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc attcctggtt
     6481 tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac atcatataaa
     6541 agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa gctggcgttg
     6601 ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca agcaacagca
     6661 acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct gctggcttat
     6721 tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct gttcttccgc
     6781 ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact ttttcttctt
     6841 cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt tgatttcttt
     6901 tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat tgttctggtt
     6961 ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac aaaagacatt
     7021 ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga ataacattac
     7081 gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag cttcttttgc
     7141 tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg cacggataaa
     7201 tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga ttcctttttt
     7261 gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta agcctaacaa
     7321 atcataagca gcacggatca actgatccaa acctgattct tcgattccta aagcttctaa
     7381 aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac gagcactgac
     7441 cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta cataatgatt
     7501 cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa ttggttttgt
     7561 cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt cgatcgttcg
     7621 ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta gttctgccac
     7681 tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt tgttgataga
     7741 ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta gtggatcaac
     7801 acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt gacaaatagc
     7861 gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt tacttgcgcc
     7921 ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt ttttcggatg
     7981 tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc ctacgttggg
     8041 gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga tcgcattaaa
     8101 caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg ccataaattt
     8161 attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc aaaatctcga
     8221 cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc tgcgcccatt
     8281 cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc aacgatatcg
     8341 tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat tgaatattca
     8401 agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg atcttacctt
     8461 tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa cgatcttcgc
     8521 tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc ttttcttttc
     8581 catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt aaattttcag
     8641 ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct aacagcgtac
     8701 gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga tccggtaaag
     8761 aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga ctagctactt
     8821 ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct tcttctaacg
     8881 gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc gcttcttcgt
     8941 caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac gctcgaaaac
     9001 gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg atgatgggtt
     9061 gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact tcttcaaatg
     9121 ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg acaacggtat
     9181 catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca tcgattcctc
     9241 ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc ttggtagact
     9301 tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact aggtgcttct
     9361 gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa gtatttacgt
     9421 acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt cagtaagact
     9481 ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt attcagtaat
     9541 tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat cgaatcactt
     9601 gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat caaaatataa
     9661 tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct tgccatcatt
     9721 gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat actcaaattt
     9781 tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag aacatcatag
     9841 atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt tccttgtgca
     9901 tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc tagattcacc
     9961 gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat ttgtgccatc
    10021 tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt gggtatttct
    10081 ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag acattcggca
    10141 aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct tgttttgctt
    10201 ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct tcttgtttag
    10261 ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga gcagtgacaa
    10321 ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg tcatgatata
    10381 aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt cgtttattca
    10441 gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt atgctaggaa
    10501 accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct acttcaaatg
    10561 ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc gtaatagctg
    10621 taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg aattgattca
    10681 tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat tcttctggta
    10741 tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct ccctacttta
    10801 acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt gaaaagtaag
    10861 aaaagccgat agactttttc tttctagtat gactcataaa atatacacag aagagagaac
    10921 tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt acgtattacg
    10981 ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg atacttaggt
    11041 ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt tggattttct
    11101 gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg atgttttctg
    11161 gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct tttttgatgt
    11221 agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc aattcttcat
    11281 tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt aaaaagtcgc
    11341 ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag tcttggactt
    11401 cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca actgcttctt
    11461 ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg tgacccattt
    11521 ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca gcacgtgaag
    11581 tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc atgacaccga
    11641 tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc aaagctgcat
    11701 tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta ccatttgttt
    11761 gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg agctggtgtg
    11821 gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact ttttctaatc
    11881 cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta gataaacctt
    11941 gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga tgacgtggtt
    12001 tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg cctacccctt
    12061 caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt tggtgagtcg
    12121 tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca ctatctggtg
    12181 tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta acagaatagt
    12241 cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg atagcaaagc
    12301 caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc ggacctgatg
    12361 aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct gtaataacaa
    12421 ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa acaccatctt
    12481 cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct ttttcgatcg
    12541 tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat gctcggacag
    12601 ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg atattcttac
    12661 ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct ggtccgccta
    12721 cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt aataatgttt
    12781 ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct gcgccaacaa
    12841 cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc cttttatttt
    12901 cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact gtcgccgaca
    12961 acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc cacaggcatt
    13021 cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc taacaacgcg
    13081 atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc gctgaagaat
    13141 aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac ggaaattgaa
    13201 aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg tgctggcaaa
    13261 tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa taattgttca
    13321 tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc tgcttctgct
    13381 aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt ttcccgaatt
    13441 ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc ttcgattaca
    13501 tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa caaatggttt
    13561 aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa cccttcccgc
    13621 agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg gatcatctgc
    13681 gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc tggataatca
    13741 atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga gcggatcaaa
    13801 gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc tgtttttgcc
    13861 cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt caagaatgct
    13921 cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa tagctgcaaa
    13981 atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc ttcccgcgta
    14041 aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg attgctcttg
    14101 ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa tcgtttgttt
    14161 ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa acgaacgata
    14221 ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa ttctaaagta
    14281 atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg cccactccac
    14341 cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg attaaattct
    14401 ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt agatgaagtt
    14461 gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc tttgcgttct
    14521 ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt aaaaaaaacg
    14581 gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa atatgtgagc
    14641 ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga tgtcgtttta
    14701 tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt tgtggaatgc
    14761 ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt aacaacgaag
    14821 aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa ctgtcatttt
    14881 gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc ttatggaaca
    14941 gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa agcggatcaa
    15001 aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact tcatcgtata
    15061 ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg tcaacgtgat
    15121 ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac taaataacga
    15181 taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa tgcagcatgg
    15241 atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc tgtccgtttt
    15301 actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt ttcacggtaa
    15361 ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat gaagacttgt
    15421 gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat gccttgcttt
    15481 tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt gacaagagct
    15541 ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt taaagctgct
    15601 tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc gttgactacc
    15661 tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc tactgtttca
    15721 gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg acccattcct
    15781 aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt ggccaaaccc
    15841 aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata aatcggcatt
    15901 agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc ttaagagcac
    15961 gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt ttgaaagggt
    16021 tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag tataatgaaa
    16081 gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg aaattcatga
    16141 tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta cttgcaaaag
    16201 taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct aagttcaacc
    16261 ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat aacgccatca
    16321 agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg ccattttctg
    16381 catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct cttgaactat
    16441 attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc atgctctttg
    16501 tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat aaaatgatcc
    16561 ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa taaacaatat
    16621 aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa ccggataaga
    16681 cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc ttcacttact
    16741 cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat tggagcgaac
    16801 ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat ccaactccgg
    16861 atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga cttcattgcg
    16921 gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat gaggctgtcc
    16981 cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc ccgtatggaa
    17041 cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta gttttctttc
    17101 cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc ctttcatcat
    17161 ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag ctgaaagaac
    17221 ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag tactcatacg
    17281 tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg ttcttttcat
    17341 atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact ttgatagtat
    17401 aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa aacttatcca
    17461 caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag ggattatcca
    17521 cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt aatccacaat
    17581 caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt ttttatccac
    17641 agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt agtggtgtgg
    17701 aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg tgtggataat
    17761 tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa aatgctaaat
    17821 tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc cctcgatgct
    17881 ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag ctataataca
    17941 tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt tgaggttcct
    18001 agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt tgaagtcggt
    18061 tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga agcagagcaa
    18121 gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct aaaaaaagcc
    18181 atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa ccagatggct
    18241 catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc gctcttcttt
    18301 tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca tcaaatgctg
    18361 caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc taatgatttc
    18421 atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg aaatgttgat
    18481 ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca agaagagttt
    18541 ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac cagtgatcgg
    18601 ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc atgggggctg
    18661 tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg taaaaaagcg
    18721 gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg acaaattgat
    18781 tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc tgcgatgaat
    18841 agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa atcaggaaaa
    18901 ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta ttatcacatc
    18961 caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc tagacaaatc
    19021 gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg agctgaattc
    19081 ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca gctgatggat
    19141 tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact aaattaattt
    19201 gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc aaaagttata
    19261 cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt tttccacaga
    19321 atcaacatgc cctattactt ttattattct ttttaactaa taataaataa acaaacattc
    19381 aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag gaattgcaaa
    19441 ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt gtaaaaatca
    19501 cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca attgaaactt
    19561 ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt tccattgttt
    19621 tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca tttactttag
    19681 aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt atcgtaaatg
    19741 gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac cagatgaaat
    19801 tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc tcacaacatg
    19861 agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct ttattagctg
    19921 tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa gcggctgatc
    19981 attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca ttaacaaatg
    20041 aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa acagaaacga
    20101 tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt ttgattcctt
    20161 caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg atcgaacggg
    20221 cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt cgtccagatg
    20281 cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc ttgagttata
    20341 cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg aaagcagcgt
    20401 tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt cgtccattta
    20461 cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct gtacgtacaa
    20521 attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga aaaaataaaa
    20581 ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt gtttaaatca
    20641 ccttt
//
LOCUS       DAZUMM010000052        20263 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000052 DAZUMM010000000
VERSION     DAZUMM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.052"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            208..2250
                     /locus_tag="ACG8E6_001764"
     CDS             208..2250
                     /locus_tag="ACG8E6_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5062175.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2261..2881
                     /locus_tag="ACG8E6_001765"
     CDS             2261..2881
                     /locus_tag="ACG8E6_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS5062176.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            2893..3351
                     /locus_tag="ACG8E6_001766"
     CDS             2893..3351
                     /locus_tag="ACG8E6_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5062177.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3370..3648
                     /locus_tag="ACG8E6_001767"
     CDS             3370..3648
                     /locus_tag="ACG8E6_001767"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5062178.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3673..5040
                     /locus_tag="ACG8E6_001768"
     CDS             3673..5040
                     /locus_tag="ACG8E6_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS5062179.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5055..6200
                     /gene="dgoD"
                     /locus_tag="ACG8E6_001769"
     CDS             5055..6200
                     /gene="dgoD"
                     /locus_tag="ACG8E6_001769"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFS5062180.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6227..6718
                     /locus_tag="ACG8E6_001770"
     CDS             6227..6718
                     /locus_tag="ACG8E6_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062181.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            7161..8360
                     /locus_tag="ACG8E6_001771"
     CDS             7161..8360
                     /locus_tag="ACG8E6_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5062182.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(8855..9613)
                     /locus_tag="ACG8E6_001772"
     CDS             complement(8855..9613)
                     /locus_tag="ACG8E6_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5062183.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            9781..10716
                     /gene="pfkB"
                     /locus_tag="ACG8E6_001773"
     CDS             9781..10716
                     /gene="pfkB"
                     /locus_tag="ACG8E6_001773"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS5062184.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            10713..12158
                     /locus_tag="ACG8E6_001774"
     CDS             10713..12158
                     /locus_tag="ACG8E6_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS5062185.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            12186..12641
                     /locus_tag="ACG8E6_001775"
     CDS             12186..12641
                     /locus_tag="ACG8E6_001775"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS5062186.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            12660..13634
                     /gene="lacD"
                     /locus_tag="ACG8E6_001776"
     CDS             12660..13634
                     /gene="lacD"
                     /locus_tag="ACG8E6_001776"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS5062187.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(13745..14306)
                     /locus_tag="ACG8E6_001777"
                     /pseudo
     CDS             complement(13745..14306)
                     /locus_tag="ACG8E6_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            14450..15064
                     /locus_tag="ACG8E6_001778"
     CDS             14450..15064
                     /locus_tag="ACG8E6_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5062188.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            15305..15688
                     /locus_tag="ACG8E6_001779"
     CDS             15305..15688
                     /locus_tag="ACG8E6_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5062189.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            16051..16560
                     /locus_tag="ACG8E6_001780"
     CDS             16051..16560
                     /locus_tag="ACG8E6_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFS5062190.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            16660..17310
                     /locus_tag="ACG8E6_001781"
     CDS             16660..17310
                     /locus_tag="ACG8E6_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062191.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            17761..18699
                     /locus_tag="ACG8E6_001782"
     CDS             17761..18699
                     /locus_tag="ACG8E6_001782"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS5062192.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            18712..19764
                     /locus_tag="ACG8E6_001783"
     CDS             18712..19764
                     /locus_tag="ACG8E6_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5062193.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            19804..>20263
                     /locus_tag="ACG8E6_001784"
     CDS             19804..>20263
                     /locus_tag="ACG8E6_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS5062194.1"
                     /translation="MRCQQHLTYVFLPKYGFYFLLLGVISDLSTPYILGLFYPKLNQM
                     TTVISVFGDVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLY
                     GIGDCIFTGLFSINTNESSWNLSTWIHNTGSGLGYAGFLLFPLLLVLLYRQ"
BASE COUNT         6421 a         3221 c         4277 g         6344 t
ORIGIN      
        1 catccaatat tctaccgaat atttaatatt cacattagcc ctaaaataat tgtcatacca
       61 tgttacattt gatataacag cgttcatttt aaaaatagct gttagtgtga caaaacatta
      121 aaccaagtgt gacaaaaaac gtgatgaaag ctttccggaa acgtatgtga acaagtatat
      181 aatgattttg tcaggaggat agtcaaaatg aataaacgaa ttcgtgaaat actcaaactg
      241 attattaaaa accctgaaat gaaactttcc actttaacaa gtgaacttga tttgacacgt
      301 cgtcaaatca attacgcgat caatcaattt aatgaagaat tgaaaacaaa gaacattccg
      361 ttaattcaga gaagtcattc tggaaatatt acggtaccaa tcgaggttat ccaaatgata
      421 tcacagcctg attacgacat aggtgatgat caggaacttg tcgttctcac tgatagtgaa
      481 agacaagcat tgatagtgat gactctgatc gctaatatcg attacatcag tctggatcat
      541 ttggtagatg cggttgatgt tagtaagacg actgttatgg aagatatcaa acgaacggaa
      601 ccgttcatca gaaattatag tctgacaatt cgatataatc ggatgaatgg ttaccaattg
      661 gttggttcgg aacaccgtat cttacagctg ttatcggatc tagtaaaacg gtttccgatt
      721 tttcgccgag aaatgattcg cagaaaattg gcacaagatg tttctgaaga agaaatcgtc
      781 catttgatcc ataacatgga acaaatgttg catcttagtt attctgatga gtcggtagac
      841 tatttgcaga catcagcgag atttttagta agccgtggga tcaagatgtc agataagcac
      901 gcgttttttc ccgatgatgt gacggatact cctgaattcc gaatgctgat gatcttggta
      961 catgaaacag gttggagtct atccaaaagt tatatggagt ggttcacgtt attattttta
     1021 acttccaata tttttgagag caaaactact caaaattatg attcggatca agaattgcga
     1081 aaattgatca gccaaatggt tgaaaatttc caaaatcaaa cgttgatttt gatcgatgac
     1141 cgagaagact ttgaacgtag gatactgagt catctgcgtc cagcctgttt tcgaatacgc
     1201 tataacttga gtttaggagt ttattcaata gatagtctga tcaaggatag taatcatgcg
     1261 attttgattg atttgatgaa agaattgatt attcctattg agaattggct aaataaggcc
     1321 tttccaaatg atgaattaga tcttttatcc tattactttg gctatcaatt aagtaatcat
     1381 aataaattga ataagcaacg acaaagggct gtagttgttt gtactaatgg tgtcatggtc
     1441 tcaaagttag tacgagccaa tatggaaaaa ctttttccag agatccattt tttagcttcc
     1501 ctatctgtgc gtgatttcta caaatttgaa gctgattatg atctggtctt tactacgacg
     1561 ccactaaaaa gttctatgct gcagtttatg atcgagccaa tcatgacata tcagcaacaa
     1621 atcagtttac gatatcgagt tttgagtgat ttaggaatta ctaaggttga tcgagcaatg
     1681 gatgaattgt taacaatcat taggaaatat gcaaatatca ccaatccagt ttcgttgcga
     1741 gaagaactgc agtacttttt gatcaaggaa gatggaagca cgccgctgga caacttcaat
     1801 gtattgcctt cattgactta ttacttgcgg ccgagttatg tacaaatcgt tgaagaagag
     1861 atgtcttggg aacgagcagt ggaggtggct tgccgaccgt tattggaaca tcaaatcatt
     1921 gactcgcgtt ttatcgctga ttgcttggat caaatcagac aaccgggata tgcaggttac
     1981 ttgggaatga agacttgcat tccccataca acggtcgata aaggtgtaat cagtgacggt
     2041 gtcagcattt tagtttgtaa aaagccgata cgatttccta atggtgaaga tgtcagcttg
     2101 attttgccgc tttcattttt tgatttaacg aagcatctgc gagccatcaa tcaaatcgct
     2161 gatatcgcca aagatgattc attgattgag cagttattga ttgctgatga cgaaaagaca
     2221 gtttatcaac tattaagaca aagatcttaa ggaggaaaac atgagactag cagattatcc
     2281 taaattgaca gtgatcatgc gtggttatac ttttgatcag gcagatgcta ttttacaagc
     2341 aatgcaggaa tttgaccatc aatttgccgt agagatgaca atgaacacaa aaggagctct
     2401 tgaaaatata catgaactaa atcaacgtta tggtgacacg acttatatcg gcgctgggac
     2461 ggtaaggaca ttagaacaag cccaagcctg ctatgaggct ggtgcaaaat ttttattagg
     2521 accacatatg ttcacaaaag aaatgttgtc ttacgccaat aagaaaggat tattggcggt
     2581 acctgccgca atgactcctt cagaagtcga ccgtatgttt aaagaaggag ctgatattgt
     2641 aaaagtattt cctgcagcgg ttgttactcc acgttttttc aaggatattc aagcaccttt
     2701 aggtaagtta ccactaatgg gggttggtgg aatcagtaag gagaatgcga aagagttttt
     2761 tgaacaaggt gctagctatt taggtcttgg ttccggtatg ttcaacaagc aagacatcga
     2821 agagctaaat gttaaaaact tagcacgttc gatgaaagaa ttaattgagg ctattaaata
     2881 ggagtgagga ctttggaact ttatttgaat gaggaactcg tttttcgaaa tgttcaagca
     2941 aaaacagata gtgaagtctt agcgttttta gcaagcgaga tgtacaaaaa aaactacgtg
     3001 aaggaggaat atattcaagc aatccaagaa cgggaaaaag aataccctac tggattacca
     3061 tcaacaccac caggaatcgc aattccccat gcaaactatg aaatggtcaa taaaacaact
     3121 ttagcaattg cgactcttaa agatccggtc ctatttcata atatggaaga taacaatgag
     3181 cagttaccaa tccaaattgt gatcatgatg gcgattgggg aaccacatgg gcaagttgaa
     3241 atgttacaaa aaattgttgg aatcatacaa gatgagccac tgcggcaaga aatgatcaga
     3301 gcaggcaatg atactgaatt attagaacta ttaaagaaag cggtttttta atataaggag
     3361 gaaatcataa tgaaacgaat tttagtagca tgcggaaatg gtatcgccac ttcaacagta
     3421 gtagcaacaa aagttagaga gtatttgcaa caacatggca ttgatgtttc aacgactcaa
     3481 acaaaattga tggaagttcc aggaaaagtt caagactatg atctattggt gacgactggt
     3541 caatttgatg gtcaaacagg tggtgtacca gtaatcaaag ggatgccgat tttaacaggt
     3601 atcggtgccg atcagacaat ggaagagatt ttgaacttac tgaaataata gctgatcaaa
     3661 ggagctgaaa aaatgaaaat cattatgaac gtctttaact tctttattga tgctggacca
     3721 acagttatgt tgccagtcat catcacgatt attggtttga tttttgggtt aaagatttct
     3781 cgtgctttta aatcaggttt gacattaggt atcggttttg ccgggatcaa gttgatattg
     3841 gactttatga cgaccaatgt tggaccagct gccaaggcga tggttgatcg aactggtgtt
     3901 aaactagatg ctttggatgt tggctggggt tcgattgcgg ctgttacttg ggcatcgcca
     3961 atcattccaa tcttgatttt tgccattcta ttggtcaata tcgttttgct gattttgaaa
     4021 cgtacacata cgctggatgt tgatatctgg aactatcacc atatggcaat cgttggtgtt
     4081 atagtgtatt ttgtgactaa gaatgtcttc ttaggtgtag gtgcatcagt agtgatggca
     4141 attgcgactt ttaagatttc agactggtcg caaccgatgg tagaaagttt cttcggaatc
     4201 cctggtgttt ctttaccaac tgtttctgcg ctttcatcac tggttattgc ttggccgtta
     4261 aattggctgc tggatcgtat cccgttattc agaaaatcaa aattcaccat caaagatgca
     4321 caaaaatatt tgggattttt tggtgattct atgatcatgg ggttagttat tgggatggtg
     4381 attggtgttt tagccggcta tgatatcaaa gcggttttac aattaggtgt cagcatgtca
     4441 gctgttttgg ttttgattcc taagatgact tcattattta tggaaggatt gatgccaatt
     4501 tcagatgctg cacaaaaatg gtcacaaaaa aaattcaaag gtacacgttt atttatcgga
     4561 ttggatgctg cagttgttgt aggtaatcca gatgttatca caacagcatt gatcattatt
     4621 ccactaacga ttgcattagc tttagtattg ccaggaaacc gtgtgttacc attcgcagat
     4681 ttagcggttg ttcctttccg cgtggcaatg gtcgttgcct tgactcgtgg taatttatta
     4741 aaaaatatca ttattggctt agttgtgaca gcttcattgc tatggtgtgg gtcagctact
     4801 gcgccaatgc tgacagcaat tgccaaatcg gttggtatta acttaggaac ttcttcaatg
     4861 ttaatctcat catttgctgc gacttccatg atccaaagtt atttggtatt tatcgcattt
     4921 gcttacaaac caatcatcgg tattccagtg ttgttattag cctttgcggg cgcatggtat
     4981 tacttcgatg gtatcaaaaa agttaatcat gaaatggatg aaccattgaa ggcacaatag
     5041 taggagggtt taaattgaaa atttcagatg tcacagtgta taaagtaaaa ccaagatgga
     5101 tttttgttaa gatctcgact gacgaaggaa tcgaaggctg gggagaaatg atctcaggaa
     5161 ctaaaacaga aacggttgtt gctggagctt atgagatagg aaaccggctg attggacgta
     5221 atccgtttga aatcgagcgc ctcttccaag aaatgcatcg atctttcttc cgaggcggtc
     5281 ctattaatgg aaccatcgtt tcgggtttag aaatggctct gtgggatatc aaaggaaaag
     5341 cgtttaatgt tccggtttat gaattattgg gcggtgcagc acgtgataaa atcaaggttt
     5401 attcttggat tggtggagat cgaccaagtg aagttgcaga acaagcacaa gatcggtttg
     5461 atcggggctt tacggccgtc aagatgaacg cgacagaaga gctgcattat atcgattcct
     5521 accaaaaagt tgatgaagtg gtggaacgag ttgcgtctat tcgcgaacgt tttggtgatg
     5581 caatggatat tggtgttgat tttcatggac gggtccataa accgatggca aaagtgctag
     5641 caaaagcttt ggagccattc catccaatgt ttttggaaga agtcgtttta cctgaaaatg
     5701 aagaacactt taaagaagta gctgattctg tggctgtacc attagctact ggtgaacgct
     5761 tgtacactcg ttggcaattc aagaacctct ttaaacaagg tactgttgat attatccaac
     5821 cagatgtggc tctttgtggg ggaatcttgg aaacccgtaa aatcgcagcg atggcggaag
     5881 cttacgacat ggctgttgcg cctcatgcgc catatggtcc tgttgcacta gcagcaactc
     5941 tgcaagtaga ctcatgtacg ccaaacgtct ttatccaaga acaaagctta gggatccact
     6001 ataacaaagg ttttgatctg ttagattttg tgaagaacaa agaagttttc caatataaag
     6061 acggctacgt agatctacct tcaaaaccag gtctgggctt ggaaatggat gaagacaaaa
     6121 tcaaagaaat tgcacaagaa ggtttgattt ggaccaatcc gcaatggaaa aattatgacg
     6181 gtacgatcgc tgagtggtga taaatgaaaa taaaggatga tgaaagatgg cagaagcact
     6241 tatgatcaca gtttttttag gactgctgat tattttgcaa ataatcgatc aaaagaaaat
     6301 tatcgtgttt gctcagagta gtcctgtaag gaagagtttg agtattttac tgacagtcgc
     6361 catcctgttg attttctggc cggaacatct gactgatcag attaaactgg ttgcttttgc
     6421 ttctgtcatt ttgatcaatg gttttttgaa ggagggtcta gcaaaagatt ctgttgttag
     6481 atttggagta ttgaacggta actttaatcg atatcctcaa gtccaagtag aaaaactagg
     6541 tgacgataaa tcatttattt cattttacaa aagcaagaat aattcttttt caatgatttt
     6601 tgataaagat caaactgaaa tgatggatgt ctttcaagaa cttgatatcc attccaaaat
     6661 tactttagat caaagttcgg aagaaaaaag agcagttgta aaaaaagata atgaatgatt
     6721 ctactaccga aattatgatt tctttatcac gaataatgaa acttgtttta atgaatcata
     6781 ttgattgaag tatacaatgt atattaagtt ttggaaagaa atatattggt acaagatata
     6841 gcaggaatcg attactcgat ccctgctttt tttaacttcc tttgcagcaa aaaaagctcg
     6901 taatctaaga gatgatggat agtttttaga atatatatat tttcttcaat tccagaaaaa
     6961 aatgaccaag ctagtatgct aaaaaagtac gatgcttcta tgatcttttt tccgtcttta
     7021 ttaacagatg ttttaattga tcaacgcata ttgtaatcta ccctaatttc catagttctt
     7081 caccactttt aaaaatagta aaacccctac aagtatgcta tagtaaattg ctaaagaaaa
     7141 accaataagg tgggagatgt gtgcgcgatc aaacaagcgg aaaaatgatt cgtttttatt
     7201 atacaagttt gcattttctt tattggatga tgttttgttc tgtctatggg tttgcgacat
     7261 tgtttttgat ttatttcaag attgatccag ctaaaatcgg agggatctta gcattggtca
     7321 atattgtttc aacggtgctt cagccttttg tcagtcaagt gatcatcaag aaaagaaaga
     7381 tcccactgat caccgtgctg aagacgatga tagcaggatt aggagtgatt ctcttagctg
     7441 gggtattgat tccgaatttg ccgatcatta cttatatctt gggtgggatt ttagtagttt
     7501 ctatgcaatc atttctcaat gcgctggcat ttgagtattc aaataccggc tacaggatca
     7561 actttggctt ttcaagagct ggtggttcat tttcttatgc tgtaacttct tttttactgg
     7621 gacagctgtt agcgatttat tctgccgcga ttttgccaat cttggttata gttgaagcag
     7681 tgatattttt cgttattttg catctgctgc cggatgtaaa aaatcaagaa gtttttccta
     7741 tgacaaaaga acaggatcgc tcagtttctt tgagaaaaaa atatcctttc ctgattttgt
     7801 tatttttagg tttcagtttt ctttttactt tccacaccat gatcaacagc tttttggcac
     7861 agatctttga aaatatccac ggcggcagta aagaagttgg catcgcattg atgatcgccg
     7921 ctttatgcga attaccggga atgatctttt ttgaacagct tgtcaaaaag aaatccagca
     7981 aattttggct gtctgttgcc agtatttcat ttgctgtcag aggattcatg atgctgctgg
     8041 ccagttcgat cctgatgatg gaaatgacgc atctcctaca agccttcagt ttcgctttgt
     8101 ttattccagc ttcggcttat ttgttcaatc aatttctggc ggatgatgat cgtgtatttg
     8161 gacaaacgat gatcatcatc gccacaactc ttggtggggt gataggaaat gtcttgggcg
     8221 gcagtctgct gcagcatttc ggtgtatggg agatgctgat tttcggtact ggttttgcgg
     8281 tgctaggtgc tttattaact attttgggca ttcgaaaaat accgaagaca ggaaacgata
     8341 gtgattctct tgttgagtag cataaaaata cttagatact agttttatat aaataacata
     8401 tagtgattat tggcgtaatt acaaaaaacg agaataactt aagtaagcat ttctaaataa
     8461 ttgagaaacc tcatctaaaa agataagaaa atatccctaa taaaacttct gtaacatttt
     8521 agataactga ttgtaatctg aataataaac tataaggaga cggttagacg gtctcctttt
     8581 tttgcgtatt ataattctag aattgttgga aattgataat aattaacaag aaatattaga
     8641 caggaacttc gaactgctta tcacattctt attattatat atttgcgtaa gtagtcctgg
     8701 tagtctcaat gcactattct atcttgaaac taagaagtta tagaaggagc aatgatagaa
     8761 agcacgagaa tacaaatagc gattaaatat ttttctagac aaccttagtt aattcacaca
     8821 gatatatttt aggtacagtc ttacagatat agagttagtg ctgtgtgaat cctgatgttc
     8881 gcagtgcata ggagtcaagt aagattctag ccagtataga aagtggaagt ctggagcgta
     8941 cctcataatc tgggaagaag gagccttctg atttaaagcc aacaaaagga atctcgatat
     9001 gttccatcaa acttgaatga cgattagcag taagcaaaat tgtcccgatc tcagctttat
     9061 aacagttttc agctgcggta gtcaattcct ttgtttcacc gtttagtgaa acaaagatca
     9121 cgatgtcatc tttggataat tttctactaa tgttttttat gatattggga tcagtatgca
     9181 tctcacaata tttaccggta agttgaaatt tgaccatcat ttcctgagca atcaattctg
     9241 aaaaacctcg ggcaaaaatc atgattcttc tagcagcctt gattcgttga atagcatctt
     9301 caatattccc gctttctacc atattgatgg tgcggattac ctcttgctca tttttaagaa
     9361 tagcagtctt tattccttca tcaactttat caagagcggc aaaattgata tttgtatttt
     9421 ctttttcttt taacgaatgt ttaaaagacg taaacccctc atatcctttt ttcttcatag
     9481 tacgaacgac ggttgctgtt gaaacgttgg catattcact tagttttaca atggaatagg
     9541 aggggatttc gagtatgtga ctttgaataa aatcccagag atatttctcc gattcactta
     9601 attttgtatc catattttgc ctccgattag ttaggataat tctattgtaa agcgggagtt
     9661 tcctttactt caagtttatt taatatattt tgtaaaagtt ttcaatgaat ttagtcattt
     9721 atgaaaacat ttacattcga tcttgataca taatgaatat gtaagaaagg aggagcgtaa
     9781 atgtcagttt atacatgtac tttaaacttg gcaatcgacc tttttatcga aacagatgaa
     9841 atgaaaccat cccaagtcaa tcgaacaaac gatgatgata tacaagctaa tggaaaaggt
     9901 gtgaatgttt cattgatcat gaagcaatta ggtatcgata gtacagcttt aggatttaaa
     9961 gcaggtttta ccgggaatta tattgaggat tatttgcatc aaaaagatat tagcactgat
    10021 tttattagcg taccgggaat gactagaatc aacgttttta cacaagttaa taaaaccaat
    10081 gaagaattca aattggttaa taaaggacct gagattcctc aggaacaaat caaaactttt
    10141 ctaaatcaaa tacaaaagct gcaagctgac gattatttgt gtgtttctgg cagtttgccg
    10201 cgaggggtct cacccgaaat tcttattgat atcagtcgta tttgttttga aaataaggtc
    10261 catctgatta ttgattcaag ctatccggaa ataattgatt gtctgccgta tcagccgttc
    10321 ttattaaaac caaacgagga ggaattgcaa tcttggtata atgtcgagct tagtgatcgt
    10381 gaaggatata ttcattatgc tgaacgcttg attgccgatg gagcgaaaaa tgttctcctt
    10441 tctctaggag gagagggagg aatattttta aataaggata tcgtttattc tgggaattct
    10501 cctaaaggta aagtagtcaa tacagcgtgt gctggtgaca ctttgttggg aacatttctt
    10561 gcaggatatt taaaaggtga acaggtagat attaatttga agaaaagttt agcagctgga
    10621 agttcaaccg cttttcgtaa aggattaaca gatttttcgg atgttgcaga gttggaaaaa
    10681 caaatcgaaa tcaaagaggg agcgagtaaa aaatgacaac ttacaaaatt gttgccgcaa
    10741 caggatgtcc aacaggaatt gcccacactt atatggctca agaagcattg gagcaggcag
    10801 ctaagcgcaa aggggtatct atcaaagttg aaacccatgg tcaagtaggg atagagaatg
    10861 aattaacacc agaagaaata aaaaacgctg acgctgtaat cattgcagct gacaaagatg
    10921 ttcaagcaga acgtttcgca ggtaagcgtg taatcgatgt gcctgtcagc aaggggatta
    10981 aagatgctga ccaattgatc gaagatgcgt tggctggtaa aggttcaatc actgcagaaa
    11041 acaaaacagt tgacgctgct ggtgagaaag cggagatggg ggttgcagca ggcattggtc
    11101 acagtattta taaaaatctc atgaacggtg tttctcatat gctgccgttt gtagtggcag
    11161 gtggggtaat tattgcaatt tcgtttgcca tctggggtgt gtactctttt gatcccaatg
    11221 atcctactta caatccgact gccgctatgt taaagggtgt tggtgatgcg gcaatgggaa
    11281 tgatggttcc tattttagca gcttatatcg ctgaaggaat cgctaaacgt cctggtttgg
    11341 ttgttgggtt tgttggcgga ctgttagcca attctggcgg agccggtttc ttaggcggaa
    11401 tttttgcagg tttcttagca ggctacttta tcttactatt gcaaaaggtc atgaaaggct
    11461 tgcctaaaca attggatggt ctgaaggcga tcttcttata tccggtgatc ggtgtcttgt
    11521 ttgttggtgt agtcatgtct ttattagcaa atccaatgga agcagtcaac caaggaatga
    11581 tgaatttctt ggcaggattc caaaattcta atccattaat tttagggatt atcgttggct
    11641 gtatgtcagc atttgatatg ggaggtccag tcaataaagc ggcttatgtt acagggactg
    11701 cgttgttggc acaaggtaac catacattta tggcaggggt ttcggcagct tgtatcgcgc
    11761 ccccattgat tactggattt gccgcactgt tttttggaaa atatttcgac acgaatgatc
    11821 gcaacgctgg tttagtcaac tttatcttgg gctcaacgca cattaccgaa ggagcaattc
    11881 catttgcggc taaagatccg ctgcgtgttc tgccaactat gatgctaggt tcatcaattg
    11941 ctgctgtact gacatatatg tttggtgtcc aagttcccgc acctcatgga ggttttctag
    12001 tattacctgt tgttactggt aaggttgcat gggtagggtc gattttactt ggttcaatcg
    12061 ttggcggagt aattttaggg ttgatccaaa aacgtagtgt agaaaaacgt gaagcagaag
    12121 cagctgctgt tgaaatgaaa gaagcaacca acaactaaag gttattctag aaagaaggat
    12181 ttcaaatgac tttgatcaaa acaaataaag tttttatgaa taaaaatttt acgacgcaag
    12241 atgaggtaat gaacttcctt gcagatcagt caattgtctt gagcattagt gaagaccgag
    12301 atgcagttta cgaaaaattg atggaaagag aagaagaaag cacgactggt atgatggatg
    12361 gttttgccat ccctcatgcc aaagatgcta gtatcaagga tgccgatatt gtgattgttc
    12421 ggttaaacgg taaggtcgat tggaaaagtt tagatggaga aggaacggat tttgtaatcg
    12481 ctctgtttat tccagattca gaggctggaa caacccatct aaaattttta tctcaggtag
    12541 ccagactttt gatgcacaaa gatgtgacag aaggtctgaa acaagcccag tctgctgaag
    12601 aaatagctga acttttaaac caaaaattag aagaaaacta aaaaggaagt agtgaaatca
    12661 tgcctttaac agaaaaaaag aaaacttata ttcgtcaact aagtaatgaa agtggcatca
    12721 tcagtgcgtt agcaatcgat caacgtggtg ccttgaaaaa aatgatcggt aaatttcaaa
    12781 ataaatcagc aaccgatgaa caaatcatca atttcaaaaa aatcatttct tctgaattga
    12841 ctccatatgc ttcagcaatc ttattagatc cagaatatgg gttgccggca gccgaagttc
    12901 gcgataaaaa tgctggtctg ttgttggcct atgaaaaaac tggttacgat gcttcaactc
    12961 caggacgttt accggatatt ttatctattt ggtctgtaaa acgtctaaag gagcaagggg
    13021 ccgatgcttg taagttcttg ttatactacg atgtagatga atctgatgac atcaatgacc
    13081 aaaagaaggc gtttatggag cggattggtt ctgaatgtgt tgctgaagat ttgccatttt
    13141 tcttagaatt ggtttcttat gatgccaacg atcctgatac ttcttcgaag gaatatgcct
    13201 tgaaaaaacc gcataaagta aatgaaatga tgaaggaatt ttctaagcct cgctatggag
    13261 ttgacgtttt gaaagtggaa gttcctgtta atatgaacta tgtggatggt tacactaaaa
    13321 atgaagttgc ttataccaaa gaagaagctg ctgcttattt caaagaacaa tccgagacta
    13381 ccaacctgcc atttattttc ttgagcgcag gagtaactgc tgaattgttc caagaaacct
    13441 tgaaatttgc aaaagaggct ggctctacat tcaatggggt actctgtggg cgcgcaactt
    13501 gggcgaatgg tgtagaacca ttcgttacta aaggtgaata tgctgccaaa gaatggatgc
    13561 agacacaagg acgcaagaac atcgaagagc tgaataatgt tttgaaagaa acagcaacag
    13621 ctattaaatt gtaagaaagc tttaactata accttaagtt tgtaacctaa tgatttttaa
    13681 taatcaattt gagcttttaa ataaagaatt ggaagcgtcc cgacgcttcc tttttcgttt
    13741 gtttttaagg catgataggt tctatgttgt cgttcaataa gcgcaagaag gtcagcattt
    13801 ttttcttaga gtatgcaaaa ttgcttttct acccaatgat ttaaaacgga gaggcaccct
    13861 tggatatggt agatggtgaa gtaacgcatt tcttctgtga tcacggtttt tttgtcttct
    13921 tgtagccaga tttttaaata attttcttcg atcagattat ttaatctctt ttgaaattgg
    13981 atatctccgt ggttgatgac gaataacttg tatacgaacg tattcgtata gacatagtcg
    14041 atcaattgag cgtatagatc ctcgtagcta tgtgtcgtat cgtaggaaat gatttcatcc
    14101 aagtcttgga aaaagctatt ttccagtttt tcataaagat catagatatc cgtataatgt
    14161 gtataaaatg tcgaacggtg aagatcagca gtttcggtca gttcacggat ggtgatgttt
    14221 tgaatttttt tatctactaa taattttgat aatgcttctg ctaacgcttt ttctgttttt
    14281 ttgacgcggc gatcctcttt tttcattttg cagcctccta atcaacataa tcaaggaatt
    14341 gtgtcggtta tccgacaatt tcaccgctat ctgaacatag ataaccgaca ccttgtctat
    14401 tataatataa gtatagcata tcaatattta aagaatagga gaataagtga tggaattagc
    14461 ctttaagcaa ctggaaaaga aagactacaa aaaagccatt caattcgcga ttaaaggtat
    14521 gcactttgat tggtatacca agaacaaact ccttttgaat ctttatggaa gatacttttg
    14581 gtatctagaa ttgaatcgcg cgacacaaat cattgcttta tatgcggatg atgagttggc
    14641 aggtgtttta ttagcagaca tcaaaggaaa atcaaaaaag catcattcat ttagtcaaaa
    14701 gttatatgtt aaactatttg atttcttaca aaatgcattt gtgaaagaca gcaaaggcgg
    14761 gtatgacgac accaatgacg aattgctttc agactattta aaaaaacatt ccccagatgg
    14821 agaaattgtc tttttagctg ccaaccctga tttaaagatc aaggggatcg gtagtaaatt
    14881 attaaaagaa tttgaacgcc gagagcaagg aaaagaagta tttctgttca cggatagcgg
    14941 ctgtacgtat caattttatg aacgacgcgg tttcgaacgg aaaaaagaaa aagataacgt
    15001 catgaagatc cttaataaag aagtagatct ccgctgtatg ttatatagta agatcattca
    15061 gtaatgtttt ttactatcag aaagcagaag taccacgaag gtcaatggaa aaagcgagta
    15121 attgatccat ttagatgaag ataggtgtag tctcaaagat attagctggc aaagatatcc
    15181 ccagttaaaa atagatttct gtatccaaca ataaaaatat ttttgtattt attagtatat
    15241 atactttaca aaaatttgta tatatactaa tttttttgta ggatcaatga aatgagggga
    15301 aaatatgcca aaatataaag aaatagcctt ggctctaaaa gagaggatcg ttgagggaaa
    15361 atatcaggaa ggttctaaat tacctgagca agaaagtctg gctagagagt ttaaaacaag
    15421 ccgtgttact atccgcaaag ctatacaact ccttattgat gaaggtttgt tatatacgag
    15481 acgtggatct ggtactttta tacgaagcaa tatcagacag gacaatgaga atgtcactca
    15541 agtaaataat gtatttggca cttcctcaca agaaaataca aatgtgacaa gtaaaataat
    15601 ccgttttgat gtccggtttc cgacagatta tgaaaggcgg ctttctgcaa cctacttccg
    15661 atccccttcc tgcaaatcat gggtataaaa aataggcata ttaagcgggc taggaggaga
    15721 aatattcaca tatcattttt tcattccgca agctgcgtca agttatttgg aatttgtaga
    15781 acagatgaga gaggtagaag cagcactaca aactactttt caataagtag gaattataga
    15841 aattttactc aaaaaatctt cattaaaaaa tctgactaaa taagttgaga aaacagaata
    15901 tacttcaata tccagtgatt tataaatatc attcactcat gatacccagc tccgtatcat
    15961 aagtgatgcg gtttttatca ttgatcctaa aaatcttagc aagacaaaaa gtacgtaaga
    16021 aagtaaagac taaatacttc ggaggaacaa atgaataaca atgataggtt attgcgattg
    16081 cgttatgcga ttgatttgaa agatacggat ttgattaaag cttttgagct aggcggtgta
    16141 ccactgacta aagaagaagc tcaggcggta ctgaccaaag tacaggataa gaacaaagat
    16201 aatgcagaaa ataatgtgta tgaaaaaacc atcaacaacc aagtttttga cgcattccta
    16261 aatggcctga ttctcttggc gcgtggtccg caaaaagata agccgatagt agacacgccg
    16321 caaaaaagca aggaaatcaa atatatcaac aatgtgttgc taaaaaaatt aaaaatcgcc
    16381 ttgtctatga ccactgatga tattttagaa gtttttgctg aggcagaact ctatccatca
    16441 aaaggcgaaa tcggggcatt tttgagaaaa gaaggccaac gaaattttaa gccttgcgga
    16501 gataaatata tgcgtaattt tctcaagggg ttaggaattt ataatcggag aaaagtataa
    16561 agaaatttca tgagaaatca aaacaatggt tgttttgatg agagctagtt tttgtatgag
    16621 attaaaatat tatcttaatc gaggagctgg attggtttta tgaatgatga atataagaat
    16681 gatgaagata agatgctttt tgaagagata gagaatcgat gtaggctcaa ttttgagctt
    16741 cggggaaaga tgtctttaat acaacaaaaa aagtatttag cgaataaatc agagtttact
    16801 ctgggacatg ttgaaaaatt aatctcggat tggattagta gtagaagcga atttacaaaa
    16861 ataaaacagc cgattaaatt tgacatgaaa aaacttttgt taaataagag tgagataggt
    16921 aatagagatc aatatattag agctaaaggg caagaaataa ttgattcatt aggtgaagtg
    16981 aggtcataca attatttgta tgtaacacat agagctgatg gaatggtcat tactgtgggg
    17041 aaatcttctt ctaatgatat ttttttagat ggagatcttt tttatcaact caatacaaat
    17101 cacttgtctg gcactgaaaa tataatttta aggacggaat atggcaatga aatatttgca
    17161 aaatatgatg agattttaaa aaattatttg gactgggctt ggataattcc ggttgagtca
    17221 ggagatgcta agaaactgga gagattattg ggcgatgaac taattaataa aaaagtacca
    17281 attttaaatt attattccca tagacaataa aaggctattt ttcatgataa tactagttat
    17341 aactgtaaaa atggaagaaa atctgggaaa gaagttttta aataagtgta ttgttcgatt
    17401 ttaataaata ataagcaata ccatccgtct gtgttgcgca acatcgtact acaatgaata
    17461 ttttttcaca aaaagaaaat catccatcga atgaatggaa ggttttagta tacacgaggt
    17521 taatgtcccc tcttttataa tcagaggagt gaggtttttt ctatgaatgt ttctattttc
    17581 ttattgctag tctagtttct cttacgatga aattgggatt ctattccagt ttttattgat
    17641 ttgctgataa aataacttga atggtggata taaacatgat aaataagaga agaataacaa
    17701 aacaaggttg tgaaatgatt ctcaaataag tataatagaa agaaaaagga ggaagaatca
    17761 atgaaaatag caattgcagg cgctggcgca atgggaagtc gctttgggtt aatgcttcac
    17821 caaagtggga acgaagtatt attaatcgat ggctgggcag aacatgtcca acaaatcaaa
    17881 gaacatggat tacaagcaaa ttttaatggt aaagaggtag aagcaaaact accaatcgtt
    17941 cttcaatccg aagtagaaaa agaagatcaa gttgatctga ttattctatt taccaaagcg
    18001 atgcagctgg aaaaaatgct gcaggatatc caatcattaa tcaaaaaaga tacagaggtc
    18061 ttatgtctat taaatggtat cgggcatgaa gatattattg agaaatttgt accaatggaa
    18121 aatatctata ttggaaatac catgtggacg gcaggtcttg aaggtcctgg tcaggtcaaa
    18181 ttatttggaa gtggttcagt agaattacaa aatttaggtg atggaaaaga agcagctgca
    18241 aaaaaattag cagacaaatt gtctgaatca ggattaaatg ctcatttttc tgacaatatt
    18301 cactattcta tttatcgcaa agcatgtgtg aacggaacaa tgaacggact atgcacaatc
    18361 ttagacgtta atatggctga gctaggaaaa acatcgactg ctcataaaat ggtggcgacg
    18421 attgtcaatg agtttgccaa agtagcagca gtagagaaga ttgaactaga tgtcccagaa
    18481 gtcattgcac attgtgaatc ttgttttgac ccggaaacaa ttgggttaca ttatccttca
    18541 atgtatcaag acttgattaa gaaccatcga ttaacagaaa tcgattatat caatggcgca
    18601 atttctagaa aagggaagaa atatggtgtt gcgacgcctt attgtgattt cttgactgag
    18661 cttgtccatg caaaagaaga tagtttgaac gtaaaataaa ggagagattg aatggaaact
    18721 tcacaaaaaa tatcaccgaa aatttttctg aataaagtcc tcgctggtac agcaacagga
    18781 atcattattg gattgattcc taatgcagta ttaggtgcta ttttaaaata ttttagtgcg
    18841 tatccctttg cggtaacgat cgcacagtta gcagttattt ttcaattagc aacacctttg
    18901 atcattggtg gtttgattgc cttacaattt ggatttgatc cgatgagaat gatggttgta
    18961 gccggagctt cttttgttgg ttctggtgta gtcaaattca cacctgattt gggtgaaacg
    19021 ggtgtttacg ttggtgctgg tacaggcgat attattaata cgatgctgac agcatcgatt
    19081 gctgtcgggc tgattttact gattggaaat agttttgggt cagtgtcgat tgtgttaaca
    19141 ccgattgttg taggtgtagg tgcaggttta cttggttttt atttattccc ttttgtaaca
    19201 gcaattactt ctgcaattgg atcattgatt aataacttca ctaccttaca accgctgtta
    19261 atgtctatct tgattggttg ttcctttgcc tttttgatta tctctcctat ttcaacagta
    19321 gcaatcggaa tggcaatcca actagatggt gtttctgctg gtgcggctgc gatgggcgta
    19381 gctgcaacca cttttgtttt agtagtgaac tcttggaagg ttaataagtc aggtgtgaca
    19441 attgcgattg ctttaggcgc tatgaagatg atgatgccta atctatttag aaaacctgtg
    19501 attcttattc cttgtttagt tagtgcagtg attacagcta ttcctgtcgc tctattttct
    19561 atttcaggta cgcctgcttc tgctggtttc ggtgtggtcg gacttgttgg tccgttggct
    19621 tctttagatg cagggctaaa tggctttctt gttgttcttt gttggttagt catccctgtt
    19681 tttgcggcac taattactca atttttatgc gagaaagtat ttaaactgta tgatcgagaa
    19741 gaagtcttca aatttttagg ctagtagatt ttactaagaa attttcaatc aatggatgcg
    19801 taagtgagat gtcaacaaca tctgacttac gtttttttgc caaaatatgg tttttatttt
    19861 ttattattag gtgtgattag tgatttatcg acaccgtata tccttggcct tttttatccc
    19921 aaactgaatc aaatgactac cgtaattagt gtgtttggtg acgtagatag tccagtcaga
    19981 cgtgctttct tggtctggtc agttgtatct ggactttttt ttgttctttc gttgcctgcg
    20041 ttgtatcacc tatttgttgg aacatcaaaa acacttgcaa tacttgctgt tgcaacagtt
    20101 ggtttatatg ggataggtga ttgtattttt actgggttat tcagtatcaa tacgaatgaa
    20161 agctcttgga atctatcgac ttggatccat aatacaggtt ctgggctagg ctatgcaggc
    20221 tttctacttt tcccattgtt acttgttctg ctttatcgtc aaa
//
LOCUS       DAZUMM010000053        20008 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000053 DAZUMM010000000
VERSION     DAZUMM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.053"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..465
                     /locus_tag="ACG8E6_001785"
     CDS             <1..465
                     /locus_tag="ACG8E6_001785"
                     /inference="COORDINATES: protein motif:HMM:NF028182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage abortive infection protein"
                     /protein_id="HFS5062195.1"
                     /translation="VQEDLKEYSASLNKVLSESVKKINEIFEDNYKDIGSYLKVFHRI
                     LKILNNRYESKDISHEEYKMYVGILRSQIEDYEFYIILINSLFTLRGTGMGIELIETS
                     FFGDELDIDLKQHFEHPEFLTEIIKDNFVEKKSYRESVKTRKKIENYLKIER"
     gene            594..1766
                     /locus_tag="ACG8E6_001786"
     CDS             594..1766
                     /locus_tag="ACG8E6_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS5062196.1"
                     /translation="MSLFDIFKQSIKNEDPSDWIPDFVFGDEESARAYLKIMAKSTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWAMSDVIYMEYNNEELERFTR
                     GLFEDYAELFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDKIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIADVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            1753..2457
                     /locus_tag="ACG8E6_001787"
     CDS             1753..2457
                     /locus_tag="ACG8E6_001787"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS5062197.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            2462..3622
                     /locus_tag="ACG8E6_001788"
     CDS             2462..3622
                     /locus_tag="ACG8E6_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS5062198.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTVEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITSSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVEYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            3698..3976
                     /locus_tag="ACG8E6_001789"
     CDS             3698..3976
                     /locus_tag="ACG8E6_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFS5062199.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            3957..4328
                     /locus_tag="ACG8E6_001790"
     CDS             3957..4328
                     /locus_tag="ACG8E6_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFS5062200.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            4344..4748
                     /locus_tag="ACG8E6_001791"
     CDS             4344..4748
                     /locus_tag="ACG8E6_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062201.1"
                     /translation="MSNVKFEGVQETIQALEKKFGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIDEVVRKNATYRNYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSEPLYFDAVGEEVKKRL"
     gene            4745..5155
                     /locus_tag="ACG8E6_001792"
     CDS             4745..5155
                     /locus_tag="ACG8E6_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062202.1"
                     /translation="MKDMLNIIYEALCLNEYIRSMTYNENTEQYRIKFYEQPETADKT
                     GPMITLRPVDTPNEAYHGSNKELSVEHMVQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFSETKRFVDARRYEGNTKIYDTEY"
     gene            5216..5803
                     /locus_tag="ACG8E6_001793"
     CDS             5216..5803
                     /locus_tag="ACG8E6_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFS5062203.1"
                     /translation="MTLVGFKKMTIGIFDSTGKIPAANLYVIEGEQDKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITALKEMVFPAAGE"
     gene            5876..6343
                     /locus_tag="ACG8E6_001794"
     CDS             5876..6343
                     /locus_tag="ACG8E6_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HFS5062204.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            6389..6697
                     /locus_tag="ACG8E6_001795"
     CDS             6389..6697
                     /locus_tag="ACG8E6_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062205.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            6892..13656
                     /locus_tag="ACG8E6_001796"
     CDS             6892..13656
                     /locus_tag="ACG8E6_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS5062206.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGITGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRDLATKLPASHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKMLKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKALDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDNTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDIVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPNDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPNAKGTNFHPG
                     GAAMVNDQKGPTYEELITLPSGEAFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYADG
                     IGNITTVSSTLNLDALILAINELTTVLRTQLMAETTNAKDTKLSDETKVADPIIPGSL
                     SEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGVAW
                     NKNLLNGWNSLTGTFISTINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYGS
                     FIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMVG
                     GMGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHEE
                     YVQMPDGRGFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDLL
                     DDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDGA
                     VNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRYT
                     AAANYAFEKFPSKIAYVITNGRVRDRMGLSGTGSSGQWTNWPDGDHFDHIHLNGSMGS
                     GDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGND
                     GLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIGK
                     GHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGDDKKRSNTT
                     NTADNSAELVTLIKQQQKQHNELMMILRAILGKDLSLKSSDIGQAANHYMGSDLNKLR
                     YTNGGV"
     gene            13660..14481
                     /locus_tag="ACG8E6_001797"
     CDS             13660..14481
                     /locus_tag="ACG8E6_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail domain-containing protein"
                     /protein_id="HFS5062207.1"
                     /translation="MFYKLQFNQNGKLFDPQRKEKIVCKEIKRQAPVYEVNYEDFEGT
                     NGSREANASFRPFELVLTFDIFYKNKHDKELLLTEFYELIFVGYQYYISYDLSPGKRF
                     KVNPTNFEITEEENDYSTIEITFSIPSGSAESSATTLTDFNLEEEWQFSQGLVAEDYK
                     YTHQTSHFIIYNAGSFEIDPREHYLRIALEGESEGNVTIFNKTTGDRFIYYPSLSTNL
                     GQTLVLDGVIPKLNGVSCGINTNHGLINLVEGVNEIEIQNITRVKSSWDFRFLYK"
     gene            14490..15533
                     /locus_tag="ACG8E6_001798"
     CDS             14490..15533
                     /locus_tag="ACG8E6_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS5062208.1"
                     /translation="MTELIIRNYEQTKEEILVGYDKGSFYENWQQNETWEIGFTVTSN
                     SLNQEVFDLVEYESSVFYNGQEFVIKEMTRKALGQLLTKQVVATHIYYTVQDGYQYNT
                     VTGAKSISQLLTHVFSAGSRGFTWEVIDPNKKFLTVEQENFGNANYLKLINEILSDYN
                     AVVIPNNKHLTFYPASEYGQQTEEQIRYKYNTDEVSFDIDTYSLKTQIKGYGKLKDGA
                     NTENPKDSDYIFTPITYTSPESQKWGIRIQDPVKDERYTVSGNMLERLKTDLQDYPNI
                     SGSVTLKWKISPNKGDHVPFIYEPLNINTYIQVVGIKTYPAIPNKPPEITLSNTKKTM
                     TSILAEMAKKGVI"
     gene            15533..16585
                     /locus_tag="ACG8E6_001799"
     CDS             15533..16585
                     /locus_tag="ACG8E6_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062209.1"
                     /translation="MGLLKLISNRISTEWKEKFNKNIDYLNDLEKKLSDQDKSTNSRI
                     DNLVLHSGGDSPNEVVDARVNHKGETFATLQGRLTDTEKKVSESVSTLEKNQADIKAQ
                     VAQLNNSVETIIGGSADWINLYVSADLGSDQIGDGSEEKPFATIQMAVNQIPLVSIPG
                     IRIWVDDGVYLEDVFIRNVSATTIHIGPKNDTSVIDPSKSDMPVKLRSLAFYQCKGFF
                     KVTGLQFVDTINAPKNSGLIYSLMLLQGGYLSVDKCKFAEDNRNLTSAAIYTEGLSAS
                     NVYNTCYFYRQNIVVYANLMSQVLISQQTSGKENTTGARSKDAIIRGKFSVGFADKNE
                     DVKDLGLIITKGTVLS"
     gene            16585..18768
                     /locus_tag="ACG8E6_001800"
     CDS             16585..18768
                     /locus_tag="ACG8E6_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFS5062210.1"
                     /translation="MAFKINESIIVIQAEATSPNRTNVVFWSHDRGTAKLRMKLVRKN
                     GIPQSLPEGTTVPIRLMFRSATAEGGYGKHDYLATIEDRVNGIVSIVLEDNILGYVGI
                     VEGSVYIDFPNDRSLDTAGRFTFDIKRSPIDDSTPELEDYYFNGFSQTIDKIEKILAD
                     GKQEIEQKITESETQIDAKLKNTNDKITKANQDVTTLNTNIDKANDRIDQTNQQISDL
                     GKLKKMYSNSIDFGNYDYSGNPNLLSKLSYDLIENQNTSAGTLSKGENSFKYNKISAE
                     VEGGVELYYKRRGIANWLPSNKTLVMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKI
                     VLDLPINSNSITQEWKEFSITGTTPTFSPQAYHPWIQFRAQDGILGEIEMSYDIKIEE
                     GSTATPFQPNLLAEPYNMCREYPNENIADHTVKFPIESGDHQIYQGYTEEELMIGQTY
                     TITLKGTKPASQTFVAYNHWTARLGELKPVDGLTDVWSLTFTPTNVVAMPKLFRVYQY
                     PRSTVGACQIDWLKIEKGNTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYAEKGLN
                     NTVSLTEPETVLGLKNFKDGVQIDGVDVATVKNLGSVLQSGSGLTTIQSDKQSAFDEF
                     SYEFERIGDRVFFHARVKTNSTTAVASHVNLLEIPLGFQFPIGWTIVGVPLSVVQWTT
                     PQGEISALVITDSTGKQVVKFATNRVGNHYISGEWRTADSYPETK"
     gene            18780..19226
                     /locus_tag="ACG8E6_001801"
     CDS             18780..19226
                     /locus_tag="ACG8E6_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062211.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGLRADGEPL
                     TIADQMFDPKLNQWIVLANTLDHNDLNNLKAMYEALEHENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPTLKGGE"
     gene            19228..19365
                     /locus_tag="ACG8E6_001802"
     CDS             19228..19365
                     /locus_tag="ACG8E6_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS5062212.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            19400..19642
                     /locus_tag="ACG8E6_001803"
     CDS             19400..19642
                     /locus_tag="ACG8E6_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFS5062213.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKNNNKWAWGYMIGLGITIIGYFLTKL"
     gene            19658..19855
                     /locus_tag="ACG8E6_001804"
     CDS             19658..19855
                     /locus_tag="ACG8E6_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFS5062214.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASALVAMLGKAYGWNGTDMIVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            19866..>20008
                     /locus_tag="ACG8E6_001805"
     CDS             19866..>20008
                     /locus_tag="ACG8E6_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS5062215.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQIA
                     NNQ"
BASE COUNT         7100 a         3181 c         4294 g         5433 t
ORIGIN      
        1 gtacaggagg atttaaaaga atatagtgca tcattgaata aagttttaag cgaaagcgtt
       61 aaaaaaatta atgaaatatt tgaggataac tacaaagaca taggtagtta tctaaaagta
      121 ttccatagga ttcttaaaat actaaataac aggtatgaat caaaagatat ttcgcatgaa
      181 gaatacaaaa tgtatgttgg catattacgt agtcagattg aagattacga attttatata
      241 attcttataa attctttgtt tactcttcgc ggaacgggta tggggattga gttaattgaa
      301 acatcattct ttggtgatga actagatatt gatttaaaac agcatttcga acatccagag
      361 tttttaactg aaatcattaa ggataatttt gttgaaaaga aatcgtatcg tgaatcagta
      421 aaaacaagaa aaaaaataga aaactatttg aaaatagaaa gataaatgat aacttattat
      481 ctcttttgag aataagatac acgttacaag atggaaataa aattattata agtaatggag
      541 tatagtccta gaaatagggc tttttatttt accttgaaag gtggtgaaaa tatgtgagtt
      601 tatttgatat ctttaagcaa tcaataaaga acgaagatcc atcggattgg attcctgact
      661 ttgtgtttgg tgatgaagaa tctgctagag catatttgaa aatcatggct aaaagtacag
      721 ttcttgattt tgttgcaaga actatgtcaa cgttagaagt taagtttaaa aataaagatg
      781 ggacatcaga ttgggattac attctaaatg tccgacctaa ttctgatatg tcagcagcta
      841 cattttggca aaaatttttt tatcggctct tagatgacaa cgaagtgtta gtgatattta
      901 cagatgataa tcaattgctg attgctgatg attttagtcg aacagagtat gccgtgtatg
      961 atgatgtttt tacaaatgtg atggtgaaaa attttgtctt tgaacgttca tgggccatgt
     1021 ctgacgtcat ttatatggaa tataataacg aagaattaga aaggtttaca cgaggtttat
     1081 ttgaagatta cgccgagttg ttcggacgga ttctagaagt tgcaatgcgc aataaccaaa
     1141 ttcgtgggtc tgtctccatt gattcaacag gaacggcaaa tgatgaaaaa ggaaaagatg
     1201 gtaagactag atctcaaaga ttacaagaat atattgataa gatttggaac tcttttaaaa
     1261 ctaactcagt tgcaattgtt cccaaagtga aaggctttga gtatgaggaa tacaccaata
     1321 aacaaggcgt atcgaatcaa tcactagatg aactgaataa gatgaagtcg tctttaattg
     1381 ccgatgttgc caacgccata ggagtaccta cggcgcttat ttatggcgaa aaagctgaac
     1441 tagattcaaa tattaaagct tttcgtaaat tgtgtattgc tccactagtt aaaaaacttc
     1501 aggacgaatt ggtggcaaaa ataattagtc gacaagagta caaaaacggg gaacgtatag
     1561 aagttaaaaa agttctccaa aaagatctat tagagtatgc tactcaaatt gacaaaataa
     1621 catctggtgg tgcgttttat attgacgaag ttcgtgatga agttgattat gatgagttgc
     1681 caaataatga aggaaaaatt cgtattcgta cgaaaaacta tgaagaaact gtgaagggag
     1741 gtgagaacga caatgccgaa agctaaaaaa gtaccatttc agtttactaa cgaaattcag
     1801 aatggtaagc acattctcac cttgagtgga aatgtacaga aaaaatattg gcgtgatgat
     1861 gatgtcataa acgctaaaga tattcgtgac tctctagata ctgtaaccga tgatattgta
     1921 atcaagttga acagcccagg cggtgatgtg tttgaaggga ttgagattta caattattta
     1981 aaagatcacc catcaaatat tactgtagaa gttacagggc ttgccgcaag cgcagcaacg
     2041 tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg ggacttcttt gatgattcat
     2101 gaagcttcta catttgcttg ggggaataaa caggacatcc aaaaaacgtt aaacgcattg
     2161 gaaacgatcg atgattctat cttagctatt tattcagata agactggcca atctgttgat
     2221 aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag cagatgaagc tgtcaaatat
     2281 ggatttgcca attctgtgaa gcgtgaagaa ccacaaaacg aacctcaaaa tattgcagcg
     2341 atgattcaag atgctgttgc cgttgcaata gcgaatttaa aggaacctgt tactaataaa
     2401 atcgaacaag aatcaaaaca aaaatcatta atcgcacgat tgcgaaaagg agaataaaga
     2461 tatgttaaaa attacagata aaactgcaga tgcaaaaaag gcatttaatg ctatttcttc
     2521 gaaagaagat gcaacacccg aacaaattaa tgctgctcta gaagaatatg taactgccat
     2581 tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa gaattgaaaa atgtgacgga
     2641 caaccgtgta ttagaggctc gtggaattcc tacactaaca gtagaagaaa caaagtttta
     2701 taatgaagcc gtaaaatctg gaggtttcga ttctgacgta gtttggccag aaacgatttt
     2761 ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt ttaggcctca ttaactttac
     2821 accaactgta ggtcgtgtca aagtaattag agcacgtcgt aaaggtgttg ctgtttttgg
     2881 accattgcat aaagaccttg aaggccaact agatgctgaa tttggtgcaa ctgaatttgt
     2941 tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat accttagatt taggaccacg
     3001 atggattgac cgttttatca atctttcttt aagtgaagca gtacgtgatg tttgggctaa
     3061 aaaaattatt acaagttctg gtaaagatga gccaattgga ttgcttaaag atctagatgg
     3121 tgcggtcaca agtggtgtat atccagataa agctagcgca gggacattaa cttttgcgaa
     3181 agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa ttatctaaat acacatacaa
     3241 agtagatggt gacgatgaag gtgaagtgga ataccgttct gttgctggac gagtgtatct
     3301 aattgttaat cctgttgatt actgggatat cgtagctaga gttacatttg caaatttaaa
     3361 caatgtgtat gggactacga tgccatttat cgctgttgat catatcatcg aatctgtaga
     3421 cgtaccagaa agtaaatgca ttgcatacgt tgataaagag tatgaagcta ctcaatcaag
     3481 agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg aaacgcgcaa ctttgtatgc
     3541 tgtcgacatg cttggtaatg gctatccaac aaataatgat gcagcacaag tatatgactt
     3601 ggactttaca gcgggggagt aacgtcccct gcaattggtg agataacacc gacgactgat
     3661 ggggcagtca ttaatctttc gtaaatgggg ttagagtatg aacactgaag aatttataaa
     3721 agaatataag gcccgatttc gtattttcca tacttccgaa gatgaggata tcgggaaaca
     3781 gttagagagc gggtttgccg atataaaatc tctaatcggc agttttgatc cttccactta
     3841 tgagaaagga aaagaattag tttatgaacg tactcggtat ctacggaacg aatcactaga
     3901 gtacttttac gacaattttc aaacaatgat tatggatgca tcaattgatc tggcaggtgc
     3961 tggatatgcc aattaaatca aattataaga agcctaaaat tgtagctggt gatctaaata
     4021 cgccagttac tttttttgag tttcaaccta ataagggtcc agaacctggt gatgaagaaa
     4081 aaaaggaact gtatttttgt acagcactag tttacaaccc atcaatgaaa gatcgtgaca
     4141 ttcttggcac aaaagaaggc gttacgataa aaatacggga tccccatcaa tcttatattc
     4201 ctactaacaa acataaagta aggatattag attatcgata tggagataaa gtttgggaaa
     4261 taatcgatgt ctcaccggat ttggaagaaa attcaattgt aaagatagta ttagaattaa
     4321 ttacttaaat ttggtgataa acgatgagta acgtaaagtt tgaaggcgtt caagaaacaa
     4381 tccaagcttt agaaaaaaag tttggagaaa agaaaactaa gacattaaca aaaaaagcaa
     4441 tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac ggacatgacc gtttttaaag
     4501 acaaaggtta tacgattgat gaggttgtac gtaagaacgc tacgtataga aattacaagg
     4561 cagaagctga aatcggttgg aatggtcctc atcagcgtta tagaatcatt cacttaaatg
     4621 aatggggata tacacgaaaa ggtaagcaaa ttaggccaag aggttttggc gtaatcacta
     4681 aatcgcttaa aaattccgag ccactatatt ttgatgctgt tggggaggag gttaaaaaga
     4741 gattatgaaa gatatgctca atatcattta tgaggctctt tgtttgaatg agtatattcg
     4801 ttcaatgaca tataacgaga ataccgagca atatcgtatt aaattttatg agcaacctga
     4861 aaccgccgat aaaacagggc caatgatcac acttcgtcct gtagatacac ctaacgaagc
     4921 atatcacgga agtaacaaag agctttctgt agagcatatg gtacagattg atgttgaatc
     4981 gaaatataga gcaacatgca agcagattca atatgagata aaaaaagaga tgaaaaaact
     5041 aggctttggc caaatttctg gtcaagggtt agatgaatac ttttctgaaa caaaacgctt
     5101 tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac gataccgaat attagaaatt
     5161 aagacacgaa aataacgtgt ctttttttat tacccaaaat taggaggaaa attttatgac
     5221 tttagtagga ttcaaaaaaa tgacaatcgg gatctttgac agcacaggga agattccagc
     5281 agcaaattta tatgtaattg aaggtgaaca agacaaaggg gcgactgtat ctgctgaaat
     5341 cagtgggcta tcaaaagaac catcaaaagt ttacggatcg aacatcgctt actatgtctc
     5401 tcaaaaagga acaggcgatg tttcagctac ttttggattg ttagatttac caacagaagt
     5461 aaatgacaaa attcttggtt acaaaaccga tacaaacaaa atcagcttct tgggagaaga
     5521 tactgagcca ccatattgtg caattttgat ggaatcggag gatttgaacg gcgatacagc
     5581 aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct attaatctta acactacgac
     5641 aaatgaagct ttcgaaccag aagcagaaga atatgtattt tcagcgattg ccaatgatgt
     5701 tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt ggagacgaag aagcttctat
     5761 tactgctttg aaagagatgg tatttcctgc agcgggggag taactagctc cgttgttgga
     5821 gcaatcactc ccactattaa tggggtaaca gttgaattaa gttaggagga taactatgcc
     5881 agatacattt aaaatctata aaaaaaatgg tactgaattt acaaaagtcg cagagggtga
     5941 aagtccactt tctattactg gaattgcagc aaatactcaa gttgctaaag gagactacca
     6001 agctacgcgt gtggttggag gtcaagaatc agtaaaagta gatattcctg catttaccac
     6061 tcttccaatt tcagttactg gtgtgacgtt ggataaaacg acagctgaag tcgaacaagg
     6121 cggaacctta aaattaactc ctactgtaac accagcaaat gcaacagaca aaacaggaag
     6181 ctggagcagt tcgaatacag ctgtagctac tgttactgga ggcaacgtta cagtaaaaac
     6241 agacgctgaa gttggcggaa ctacagaagt ttcatttact actacggatg gcggtaaagt
     6301 agcaaaatgt actattacag ttattgaaaa agcagcgggc tagtcttcgg actagctttt
     6361 ttatttggaa aataatggag gaatctaaat ggcaaaagta agaattgaat tgaaagaaga
     6421 atcaggaaag aaagtctatg aaaacataga cacaacaggt aaagattatc gaaaagcttt
     6481 agaaacaatc aaaaagctaa atgaagatgg agtaatgatt tggaatcgtt tagacattta
     6541 tttggatttt gctgtaggta tcttcaggga tagcaaattg acagctgaac aaattcttga
     6601 tggcttacca tctgagaagg taatgagcac attggacgac atcctaggag aagtaatggg
     6661 gattgaaagt aatccagacc cagacgcaaa aaagtaacac ccgaagaagc agaagaaatg
     6721 tacttaaacc tatgcagaga gttagtgaaa gcgggatggt cactatctga catagaagat
     6781 aattcctttg aaatgatgat gaaaattgcg tgcacttcgc ctaagaaaga gaaacaaaaa
     6841 gaagttgatc ttaaagactt cttgaaatcc atgtagagag gaggtaaaca tatggcaaat
     6901 ggaaaacctt taggcaatat gattataaaa ctagacttag acagttcagc tttttcaaaa
     6961 ggtctagccg gagctaaaaa cgctgttaac catcaaatga aagcaatgaa atcacagatg
     7021 caagtaatga actcttcagg gaattctctc ggtgctttac aagcaaaata taatggttta
     7081 ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc tcactaaggc ttataaggat
     7141 agttttgact ctaatggaaa cgctactgct tcgactgcta aatacgctaa tcaattgaat
     7201 caagcaacag ctagatcagc aagttacgaa tctcagatga aaacaaccgt tggacagatt
     7261 gctcggatga aagtggaaac ggaagggatt acggggaaac taaaagctaa ttctgatgta
     7321 ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag gagtttctgg gataggatct
     7381 gccctaactg ttggagtaac agctccaata gtggcaggtg caactgctgt tactagtgca
     7441 gctatttctt gggaaagtgc ttttgcagga gtaaaaaaga caaatgatga agttgtagat
     7501 tcgaatggga acgtagttta ctcctacagt gatttagaaa aaggccttcg tgatttagcg
     7561 actaaattgc cagcaagcca ccaagaaata gcgaacgttg ctgaagcagc aggtcaatta
     7621 gggattaaga cagaaaatgt agttagtttc accaagacaa tgattgatat gggcgaatct
     7681 actaatatgt cagcagaaac agctgcaact tctttagcac gatttgctaa tataacccaa
     7741 atgtctcaaa aagactttga taaattagga tcagtcattg ttgatctagg taacaatttt
     7801 gctacaaccg aatcggaaat cactgaaatg ggactgcggt tagcgggtgc tggtaaacag
     7861 attggaatga gccaaggcga aattctagga tttgctactg ctctatcctc tgttggtgtc
     7921 gaagctgaag caggtgggtc tgccttttcg aaggtcatga tccagatgca actagctgta
     7981 gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg cagaagaaca aggtgttccg
     8041 tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat cgttaaaagc agtatcagag
     8101 caaatgggat tcacttcatc tgatttaaag aaaatgtata aagaagcgga caattcgaaa
     8161 agttcgcttg aaaattttgc gaatgtggca ggtgttacaa gtgaacaatt ccagaagttg
     8221 ttcaagagtg atccttcaga agcaatcatc aagttcattc aaggactgaa agattcagaa
     8281 aaacatggga cttcagcaat caaagttctg gatgacatgg atataaaaga agttcgacta
     8341 cgcgacagtt tattgagagc tgcaaatgct agtgacatat tcaaaggagc aattgaaaaa
     8401 ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg aagctaataa aagatacgaa
     8461 acaactgaat caaagctaaa gatgttgaaa aacgaagtga ttgatacagc aatcgatctt
     8521 ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg tatcaaaacc gttgataaaa
     8581 aacttaggga atatggcgaa agctttctca gccatgagtg atgatcaaca aagaaacatt
     8641 atcaaatggg ttggattagc agcagcagct ggtccagcgc tgaagctttt aggaactggt
     8701 acttcagtaa taggtaaagt tacttcaact actggaagat tgacgaaaag ccttgttgat
     8761 ttagcagcga aagcagcaga aaagaaggct ctggatgcct ttgcatcgtc ggtaacaaca
     8821 gctggtgctg cagctgcaaa gacagcagga gctggaggta ttggcgggtt agtccctatg
     8881 atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg ctagcggaat aggcgcaatg
     8941 acaacatcac taggtttgtt aggtcctgct ctacttggta ttgttggcgt tggtggcgca
     9001 ctagcagttg gctatggagc atggaaatta tttggtgaag aagcttggaa ttcctcgcaa
     9061 agagtaaagc aatggggaac agatgtcggt gaggaaatag ataatactct tgacggagta
     9121 cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc tcaaagaggg ttttactgtt
     9181 gatgatgcga ataatatggc ggctaatttc gcaaccgctg gaacatcttt ggaaacatct
     9241 ttaacaggta gaataaaggc attagacgat gcactaaaag ggctacctca atcagttcag
     9301 gatgccatga aagaagtagc tgaatcagaa gaagaaacct tagggcaatc tctttctaaa
     9361 ataaaagaca acaatgaaga aataaaaaaa atcagagaaa gagccgcaaa tcagaacaga
     9421 gacttgactg tttcagaact acaacagata caggatctga tgaaaggaac ttctcaagcg
     9481 tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa agatactatc agccatgaat
     9541 ggtgatgttg cacaggctac agaagaagaa gcgaagacgt ggttacaatc tttagcgaaa
     9601 caaaaagctg caactaccga aaattataga caaatgcttg aagaaaaaaa ggcggcttta
     9661 aaggaaaagg gagtaagcaa agaattcatt gatgcattag aagatgaaat gaatgattac
     9721 atcaaaacta caaatcaagg attcgatagc caaatagctg ctataacaaa taaatatcca
     9781 caattgatta acgaagtgag tttatcaaat ggtcaattat ttagcaaaac ggcagctgct
     9841 atggacaata ctggtcagta tacaaaagtt tttctcgaaa gtaatgaaaa gattttagcc
     9901 caagcgaaga aaacatcgga tgaaatagca gataatgccg aaaaaaatgc agaacaactt
     9961 ggtatggtag ctgatgaggc tacgcatgct ggatcaactt ggaatggatt agttttagac
    10021 gaaaaaacag gaaaagtaaa aactaacgta caagaagtag tgaatgaagc aacgaaggat
    10081 ttcactaaat ggaatgaact taaacctgtt gttcatgatg cgaaactaac aacgaacgca
    10141 aaagatgtca tttccatagc ggcagtacaa aatggttatt gggatgatat ggaatggaaa
    10201 gaaaaagaag cattattaga agatgagtgt tctaaaaatg tatacaaggc tttagaaaat
    10261 gctggtaaat ggaatgagtt agatattcct tctaaagaag caatcctaac ttcaaatact
    10321 cctgaagtta tgggtgaaac tctcatgaat ttgggactat gggatactta caaacttcaa
    10381 gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg ctatcgatgg atcagaagaa
    10441 aaattaaata gttgggctca attagataac cccacaaaag aattgttatt agataactca
    10501 gatttttcta caaaactttt taattccgaa gaaatgttta aacgttggaa tcagttatca
    10561 tctaatgcaa agtttctttc agctaataat actaaattta cagaggttct tttatcatct
    10621 gacagaatgt ggaatcaatg ggtaaacctt cctgataatg agaaaagatt acttgcagat
    10681 aacgaagatt tagctaccac tgttttatct tcagaagaaa tatacggaag atggactcaa
    10741 ttaccagata atcaaaaata tatgttagct gacaatactg acttagcaaa caaaatattt
    10801 gcttcagaag aatcatggaa tgcatggaaa gcaattccag acatagttaa aaacttgctt
    10861 ggtaataata ttgatttact ggctaaagtg aaagatggga ctgtaagtgt agaagaatat
    10921 aacaaagtta tgccacttct taaaactcta tatggagata acgttaattt aaaagaggca
    10981 atcagtgggg ctaaaagctc tatagagaat tacaacaaaa atatttttcc taatgataaa
    11041 actgcagtag gacacgatga ggcttcacaa gctgctaaag atgcatttga agcgttcaac
    11101 atcttccaaa caaaaatacc agataaaatt actaaaactg tatcagctga ctttataggt
    11161 cctatgccta atgctaaagg aacgaacttc catcctggcg gagcagctat ggtaaacgat
    11221 caaaaaggac ctacttatga agagcttata accttgccaa gcggagaagc ttttatacct
    11281 aaaggacgta atgtcgtttt agacttacca agaggttcga aagtactgaa cgccactaaa
    11341 actaaacgtc tagtacctaa atatgctgat ggtataggaa acataacgac ggtttcatct
    11401 acgctaaact tagatgcttt aattttagct attaacgagc taacgacggt attgagaact
    11461 caactgatgg cagaaacgac taatgctaaa gatactaaac tatcagatga aacaaaagtt
    11521 gccgatccga ttattccggg cagtctttca gaaaaatcag atcagtatct aggaattgga
    11581 gctcaatggt taaccaatct aatgaatggt tggaactcag cggttcctca atatatgaat
    11641 agtgaaatgg tttttattac aaactatctc aatgcgctaa aacttcaaaa caatcctaat
    11701 tatacgcaag gagtagcatg gaataagaac ttactgaatg gatggaatag tttaactggg
    11761 acatttattt ctacgatcaa ttcattttgt aatcaagcaa tggtcacgct tagaaactac
    11821 aacactccta tgtacaacaa tggccgaacg tggcagcaaa ataatcttaa tggttggaat
    11881 tcattgtatg gatcattcat agcccgtgta aatcagcttg gtaacgattc gattaacaac
    11941 cttcgttcta aaagtggtgg tttttacaat gcagggacat tcttgttgca atctttaata
    12001 aacggaatga attcgttagg taattctctt tctacaacaa tgaacaatgt cgctaataca
    12061 atggttggtg gtatgggcaa aggggttaat ggcgtcatat cgggagttaa ctatgtgctt
    12121 aaggaagtcg agtcaagtaa gaatatcgga aactggccaa ttccacaata cgctaaggga
    12181 actgatggac atcctggtgg acttgctatg attaatgatc aaaaaggcct agttcacgaa
    12241 gaatatgtac agatgccaga tggacgtggt tttattgcca aaggtagaga ccttcttgtt
    12301 aatttgccta aaggtgctca ggtcttaaat gcttctttaa caaagaaatt aaaggaacgt
    12361 ttaaatattc cacactacga aaaaggtatt ggcaatttag atattgttga tttgttagat
    12421 gatgaaaaaa gaatgttaga attcttgact agcaaagttg acttttcagg cattaatgaa
    12481 ccatggcttg atatgactaa atcaggaacg tcattaatgt ctaaagctgc aaatacaatg
    12541 cttcaatcaa aattaagtga attcttcact catggcaatt ttgatggtgc agtaaatgcc
    12601 aatggtgttt atcaatattt agttgatgtt gcacagaaag taatgggtaa gttccccgga
    12661 cttacagtaa cttctggata tcgagcagga gatgcttact atcacggaaa acggcaagct
    12721 atagacttgg cttatccagg tatatctgga gatccgagat atacagcagc agctaattac
    12781 gcttttgaaa aattcccttc aaaaattgcg tatgtcatta cgaatggacg tgtacgtgac
    12841 cgcatgggat tatctggaac agggtcaagc ggacaatgga cgaattggcc agatggcgat
    12901 cacttcgatc atattcattt aaacggttcg atggggtctg gcgatatatt caatggagga
    12961 gttggaggaa gtggtgttgc acgctggcgt tcttatgtgt ctaaagcatt gaaaatgaat
    13021 ggattaccag ctacagcagc ttatataaat gcctggatgt cccaaatcca aacggaatct
    13081 ggtggtaatg agaaagcaat cggcggcaat gatgggctcg ctgaaggcaa tgcaacagga
    13141 ttacttcaaa cgaaacccgg aacttttgca gctaacgctt ttcctggtca cggaaatatt
    13201 atgaacggat ttgataacat gttagcagca atcaattatg ctaagaaacg ttacggagtt
    13261 gcaggtatgt tgcaagttat cgggaaagga catggatatg ccaatggtgg attgattacc
    13321 aaagacggac tatatcgagc gggagaagga aataaaccag aaatggttat tccattgaca
    13381 agaaaaacaa gagctattga attgatgggt caagcattag cttttctttc tggcgatgac
    13441 aagaaacgct ccaacactac aaatacagct gataattcag cagagcttgt aactttgatt
    13501 aaacagcaac aaaaacagca taatgagtta atgatgattc ttagagcgat tcttggaaaa
    13561 gatttaagtc tcaagtcttc tgacattggg caagctgcta atcattatat gggctctgat
    13621 ttgaataaac ttcgatatac gaatggaggt gtttgataat tgttttacaa attacagttc
    13681 aatcaaaatg ggaagttgtt tgatccgcaa agaaaagaaa aaattgtatg taaagaaatc
    13741 aaacgtcaag caccagtgta tgaagtgaat tacgaagatt ttgaagggac gaacggtagc
    13801 agagaagcta atgctagttt tcgtcctttt gaattagtgc tgacttttga tatcttttac
    13861 aaaaataagc atgataaaga actgctatta acagagttct atgagctaat ttttgtcggt
    13921 tatcaatatt acatttccta tgatttaagc ccaggaaaaa gatttaaagt aaatccaaca
    13981 aatttcgaaa ttactgagga agagaacgat tattctacga tagaaataac ctttagtatt
    14041 ccatcaggga gtgctgaatc cagcgcaacc actttaacgg atttcaatct agaggaagaa
    14101 tggcaattct cgcaaggtct agtggcggaa gattacaagt atacgcatca gactagtcac
    14161 ttcattattt acaatgctgg aagctttgaa attgatccac gtgaacatta tctacggatc
    14221 gcattagaag gagaatcaga aggaaatgtg acgattttca ataaaacaac tggcgatcga
    14281 tttatttact atccatcact ttctacgaat ctcggacaga cattagtctt ggatggcgtg
    14341 attccaaaat tgaacggtgt aagttgtgga atcaatacga atcacggtct gatcaatttg
    14401 gttgagggag tcaatgaaat cgagatccaa aatattactc gcgtgaaatc ttcatgggat
    14461 ttccgtttct tatataagta ggtgattgag tgactgagtt aattattcga aattatgaac
    14521 aaaccaaaga agaaatcctt gtcggttatg acaagggttc tttttatgaa aactggcaac
    14581 aaaatgaaac gtgggagatt ggctttaccg ttaccagcaa ttcgttgaat caagaagtat
    14641 ttgatttagt cgaatacgag tcttctgttt tctacaacgg acaggaattt gtgatcaaag
    14701 aaatgactcg caaagcactt ggacaattgc tgacgaaaca agtagttgcg acacatatct
    14761 attacaccgt tcaagatggt tatcagtaca acacagtgac aggtgcgaaa tctattagcc
    14821 agttactcac gcatgtattt agtgcaggga gtcgcggctt tacatgggaa gtcattgatc
    14881 caaacaaaaa attccttacc gttgaacaag aaaatttcgg taacgcgaat tacttgaagc
    14941 tgatcaatga gattctatct gactataatg cagtcgtgat tccgaataat aaacatctaa
    15001 ctttctatcc tgccagtgag tacggccagc agacggaaga acagattcgc tataaataca
    15061 atacagatga agtgtcgttt gatattgata cgtattcctt gaagacgcag atcaaaggct
    15121 atggaaaatt gaaggatggt gcaaatactg agaatcctaa agatagtgac tatatattta
    15181 ctcccatcac ttatacaagc cctgaatcac agaagtgggg aatcaggata caagatcctg
    15241 ttaaagacga acggtatacc gtatcaggga acatgctcga gcggttaaag acagacttgc
    15301 aagactatcc aaatatttcg ggatccgtaa ccttgaaatg gaaaatcagt cccaacaaag
    15361 gcgatcacgt cccattcatt tatgaacctt tgaatattaa tacgtacatt caagtggtag
    15421 gaatcaagac gtatccagcg ataccaaata agccaccaga aatcacattg agcaacacaa
    15481 agaaaacaat gacgtcgata ctcgctgaaa tggcgaagaa aggagtgatt tgatggggtt
    15541 attaaaatta atcagtaacc gtatctctac ggaatggaaa gagaaattta ataaaaacat
    15601 tgactacctt aatgatcttg aaaagaaatt gtctgatcaa gacaaatcaa caaacagtcg
    15661 tattgataat ctcgtgcttc attcaggagg tgattctcct aacgaagtag tggatgctcg
    15721 ggtgaatcac aaaggcgaaa cattcgctac gttacaagga agactgacag acaccgaaaa
    15781 aaaagtttct gaaagtgtct ctaccctaga aaaaaatcaa gctgatataa aagcacaagt
    15841 cgctcaatta aacaatagtg tagaaacaat cattggtggt agtgctgact ggataaatct
    15901 ttatgtttca gctgatttgg gaagcgacca aattggagat ggatcagaag aaaaaccatt
    15961 tgcgacaatt cagatggctg taaatcagat cccgttagtt tctattcctg gaattagaat
    16021 ttgggtagat gatggtgttt atttggaaga tgttttcata agaaatgtta gtgctactac
    16081 tattcatatt ggccctaaaa atgatacatc tgtaattgat ccatcaaaat cagatatgcc
    16141 agtaaaactt cgcagtcttg cattttatca atgtaaaggt tttttcaagg tcacaggtct
    16201 tcaatttgta gatactataa atgcacccaa aaatagtgga ttaatatata gtctgatgtt
    16261 gctacaagga ggatatcttt ctgtcgataa gtgtaaattt gctgaggata acagaaattt
    16321 aaccagcgca gcgatttata cagaagggtt gtctgcttct aacgtctaca atacttgcta
    16381 tttttataga caaaatattg tagtttatgc caatttaatg tcacaagttc ttatatcaca
    16441 acaaacaagc ggcaaagaaa acacaacagg tgctaggagt aaggatgcaa tcattcgtgg
    16501 gaaattctca gtgggttttg ctgataaaaa tgaagatgtt aaagatcttg gcttaatcat
    16561 tactaaaggg acggtgttgt cctgatggcg tttaaaatta atgaatcgat tattgtaatt
    16621 caagcagaag ccactagtcc aaacaggacg aatgttgttt tttggtcgca tgatcgagga
    16681 acagctaagc ttcgaatgaa gttagttcgg aaaaacggca tccctcaaag cttacccgaa
    16741 gggacaactg ttccgattcg tctgatgttc agatctgcaa cggcagaagg tggttatgga
    16801 aaacatgact atctcgccac cattgaagat cgtgtaaatg gtattgtgtc cattgtgctg
    16861 gaagataata ttttaggcta tgttggaatt gtcgaaggta gtgtttatat tgatttccca
    16921 aacgaccgct cgttagatac agctggtcgt tttacttttg acatcaaacg cagtccaatc
    16981 gatgatagta caccagaatt ggaagattat tatttcaatg gtttcagtca gactattgat
    17041 aaaatcgaaa aaattctagc tgatggaaag caagagattg aacagaaaat tacggaatct
    17101 gaaacgcaga ttgatgcgaa attaaaaaat acaaacgaca aaatcacgaa agccaatcaa
    17161 gatgtcacaa ctctcaatac gaatattgat aaagcaaatg atcgtattga tcaaacgaac
    17221 cagcaaataa gtgatctcgg aaagctgaaa aagatgtact ccaacagcat cgatttcggg
    17281 aactatgatt atagtggaaa tcctaatttg ttaagtaagc tatcatacga cttaattgaa
    17341 aatcaaaata cttcagctgg aacactttct aaaggtgaaa actcgtttaa atataataag
    17401 atatcagctg aagtggaagg tggagtagag ctatattata aacgaagagg tatagctaac
    17461 tggctaccat ctaataaaac gcttgtaatg actgttaagc ttagagctgg agcggactat
    17521 agtccagttg acggaaaact tatactgatt agatataggt atgttgacag tggaactggc
    17581 aagattgttt tagacttacc tattaacagt aattcgataa ctcaggaatg gaaagagttt
    17641 agtattactg gaactactcc aacatttagc ccacaagcat accatccttg gatacaattt
    17701 agggctcaag atgggatact tggggaaata gaaatgagct atgacatcaa aatcgaagaa
    17761 ggctcaacag ctacaccatt ccaacctaac ttattagcag aaccttacaa catgtgtcgc
    17821 gaatatccta acgaaaatat tgccgatcat acagttaagt tcccaatcga atctggcgac
    17881 caccaaatat atcaaggtta cacagaagaa gagcttatga taggtcaaac gtatactatc
    17941 acgcttaaag gaacaaaacc cgcaagtcaa acctttgtag cgtataatca ttggactgct
    18001 cgtttaggag aactaaagcc ggttgatggg ttgacagacg tatggtctct aacattcaca
    18061 ccaacgaatg ttgtggcgat gcctaaactt ttccgtgttt atcagtatcc acgatcaaca
    18121 gtaggcgcat gccaaattga ctggctcaag attgaaaaag gcaacacccg aacgcccaat
    18181 attagtcagt ttaaatactt cggtgaaggc ttgaaagata gtaacaatcc gaacgactac
    18241 agctgggata tcacacctga atatgctgaa aaaggcttga ataatacggt tagtctaact
    18301 gaacccgaaa ccgttttagg acttaaaaat ttcaaagatg gggtccagat cgacggagta
    18361 gatgtggcaa cagttaaaaa cttaggtagt gtgttacaga gtggatctgg tttgactact
    18421 attcaatcag ataagcaaag cgcatttgat gaattttcat acgaattcga acgtattggt
    18481 gatagggtat tctttcacgc acgagtaaaa acaaactcga caacagccgt agcttcacat
    18541 gtgaatctac tagaaattcc tttaggattt cagtttccta ttggatggac aattgtaggg
    18601 gttccgttgt cggttgtaca gtggactacg ccacaaggag aaatcagcgc tttggtaatc
    18661 actgattcaa ctggtaaaca agtagttaaa tttgcaacta atcgagtagg aaatcattat
    18721 atttcaggag aatggcgaac agctgatagt tatccagaaa caaagtagga gggaaataaa
    18781 tgaaaaacat ttggaaatac ggacgtactg gcggagagta tgcaggaaaa gtattggacg
    18841 atatgcttgt atccgttcct tacacggatc agccaccgct cgaaggatta cgtgctgatg
    18901 gcgaaccgct aacgattgct gatcagatgt ttgatcctaa actgaaccaa tggattgttt
    18961 tagcgaacac actggatcac aacgatttaa acaatctcaa agcgatgtac gaggcactgg
    19021 aacacgagaa cggcgattta aaacagctca acgccaaact catgctaagc gatgtagcaa
    19081 ttaaacagga aaatactgca ttgaaagaaa aagcggatag tttagcacaa atcaattcaa
    19141 aaatgatgct tgcttcgtta caaaatagca aagacatttc agaaattaaa gagcaactaa
    19201 atccaacttt aaagggaggt gagtagtatg tttagtttta gcgatgtgaa aatgatgtat
    19261 gattggggct gttttactga cgatcaagtt cgactattcg ttccactatg cattacagac
    19321 gaagaagcag ataaaatcat tagcaaagaa gagagcgcat cttaagtgat gcgttttttt
    19381 gttggaaagt tggtggaaca tgaaagaaga agcgctccaa gacgttgtgg agagattagt
    19441 aagaattgaa acaaaattag acaactacga atcacttaga gaaaaggctg atagtgcaaa
    19501 agatttagca gataaagcct attcagtagc actaaacaat gcagaagaca tcaaggaaat
    19561 gaagaacaat aataaatggg cttggggcta tatgattggc ttaggcatta cgatcattgg
    19621 ctatttcttg actaaattgt aaaggaggtg agaagaaatg attttaccag ataagtatta
    19681 tcaagtcatt aaatggacag ttttaacagt attgccagct gcatctgctt tagtagccat
    19741 gttagggaaa gcgtatggat ggaatggaac agatatgata gtgttgacta tcaatgcagt
    19801 agcaacattt ttaggcgtta tcactggtgt gtcggcttat aatttgaaaa aataggagga
    19861 aacaaatgaa aaagaaaatt actattactg cgatgagcct attaacggct ctttttttat
    19921 tgccaattaa tgggtttgcc tatactatca acaatgaatt taatttgggc gcaaatgaag
    19981 gtagctcaca aatagcaaat aatcagta
//
LOCUS       DAZUMM010000054        19021 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000054 DAZUMM010000000
VERSION     DAZUMM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.054"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..193)
                     /locus_tag="ACG8E6_001806"
     CDS             complement(<1..193)
                     /locus_tag="ACG8E6_001806"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062216.1"
                     /translation="MRIKGKNISWVFYMLGILAFTFVAIKLSEIIIPNDSVVSKNLVL
                     ERAKLIISILGPALTLIVFV"
     gene            complement(225..1916)
                     /locus_tag="ACG8E6_001807"
     CDS             complement(225..1916)
                     /locus_tag="ACG8E6_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFS5062217.1"
                     /translation="MLFNQHVDQYIRKWKSGELILNEKRIQLLKLIEKEILPRDDIYY
                     FDEEQINNYIEFSQAWYFELDEWEKFISAFIFLFYKEDDEVVFDEFVINMGRGGGKNG
                     FISTLANYFVSSLHGIDYYDVSIVANSEKQAKRSFQECYRVINKKGNEDLKEEFEAYK
                     SSLTGLETQSVFEYKTSNASSQDGGREGAVIYDEYHEMENTDIVDVFSGGLGKVDHGR
                     QFFIGTKGFVREGYFDIKYRECEDILNGLSEFKGVFPYICELDAIEEMDDPKNWPKAN
                     PALQPPLNKRGKRLFNEVMKKYKKLAKEPSGRSAFVTKRMNFLEDNMENSVASREEIM
                     ATNRPFFELDTVPIGSLDFGSVRDFATCGLLFKNRKKSEYAFKSFTFAMKNFCDVHYG
                     YSLKEQLVGSEKRAPIKTWEKMGLMKVIDEPSLNPKYIVDWFIQAREEYGVRVIVVDN
                     YKADILGPMLEKEGFEVVRLKRPSSLHPLLAPRIEDGFANHKFIFGDNPLMRWFTNNV
                     YVKETQAGKQFLKKEEVKRKTDGFQAFVHALYKATELDEQIDYDDAFDMLDEIEF"
     gene            complement(1919..2557)
                     /locus_tag="ACG8E6_001808"
     CDS             complement(1919..2557)
                     /locus_tag="ACG8E6_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016630802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5062218.1"
                     /translation="MDGVNSANATYLFYPSPLRRPFSKYRRKVIGLNSKLVGTRMKQK
                     RIEKGLTQADVATKSGISSRYYGSIENGKNSPSIETLNSISIVIGCSLHFLLNDRMDD
                     MEKRVELEKKRLQKIFDKIPHDKLNLVEGLIIQAARLRVLLDDNWKDIIENGEYEKFK
                     QSENQIAYDRKRPIVENYDNRDKTYQAIVKQLTDLLPQGTRADKKSKLLGRK"
     gene            complement(2641..2901)
                     /locus_tag="ACG8E6_001809"
     CDS             complement(2641..2901)
                     /locus_tag="ACG8E6_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062219.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEETTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            complement(2892..3203)
                     /locus_tag="ACG8E6_001810"
     CDS             complement(2892..3203)
                     /locus_tag="ACG8E6_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS5062220.1"
                     /translation="MIDVSTKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            complement(3204..3776)
                     /locus_tag="ACG8E6_001811"
     CDS             complement(3204..3776)
                     /locus_tag="ACG8E6_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062221.1"
                     /translation="MKLKDYIREGYNIVTTPNLAYKIQEDYPNVLVFTDRALDAIPIG
                     KLLVDRYYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWHTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            complement(3817..4101)
                     /locus_tag="ACG8E6_001812"
     CDS             complement(3817..4101)
                     /locus_tag="ACG8E6_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062222.1"
                     /translation="MNDNYDYIKLIEKIRAEKDMDELANLFMNIISLVGLKMDEVAAL
                     NYFIAEQTIRAEHNAKFLKDRLDLDVKGLGVEGIFKVQEALVNVYVEKMQ"
     gene            complement(4098..4325)
                     /locus_tag="ACG8E6_001813"
     CDS             complement(4098..4325)
                     /locus_tag="ACG8E6_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062223.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIIEMTREAT
                     PNEIDKYNLRYCGHGYWKDEYIQQNIERYIK"
     gene            complement(4332..4544)
                     /locus_tag="ACG8E6_001814"
     CDS             complement(4332..4544)
                     /locus_tag="ACG8E6_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062224.1"
                     /translation="MNEKQLSELFKLNESNQTAEATFYEAQKSLALIAKQAKYFYDQL
                     ILQGFNEGQAMEFMMRTFSASNQQKE"
     gene            4903..5151
                     /locus_tag="ACG8E6_001815"
     CDS             4903..5151
                     /locus_tag="ACG8E6_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062225.1"
                     /translation="MKITAAITKEQGVTFTVVVVKNGVISSSHREQVRSSIPRNFPRP
                     IILAEQSHGKMKYHGRTDIVKFLSNVPYQALPWKDYTL"
     gene            complement(5105..5365)
                     /locus_tag="ACG8E6_001816"
     CDS             complement(5105..5365)
                     /locus_tag="ACG8E6_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062226.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIAIVNKAE
                     SENSSFFVKANWNIDLPEGDINNPVAEPIKYNPSKAKLDKVH"
     gene            complement(5627..6043)
                     /locus_tag="ACG8E6_001817"
     CDS             complement(5627..6043)
                     /locus_tag="ACG8E6_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS5062227.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            complement(6303..6596)
                     /locus_tag="ACG8E6_001818"
     CDS             complement(6303..6596)
                     /locus_tag="ACG8E6_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062228.1"
                     /translation="MELKIDKVTFEEPAFQEMDERIAELVEKKQDELIALLEKKYQFP
                     KYMNKMQAAKYLGVSYNTMMDKYVPAGLKMGIVNGVIRISKEECDRFMKEHQK"
     gene            complement(6580..6885)
                     /locus_tag="ACG8E6_001819"
     CDS             complement(6580..6885)
                     /locus_tag="ACG8E6_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062229.1"
                     /translation="MKLKDGFYTDAYGIGGLMMDLPTKNPIKQEESEIKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVINNGIKN"
     gene            complement(6882..7085)
                     /locus_tag="ACG8E6_001820"
     CDS             complement(6882..7085)
                     /locus_tag="ACG8E6_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFS5062230.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASETIRKFGISILNDDRKWEAER"
     gene            complement(7092..7322)
                     /locus_tag="ACG8E6_001821"
     CDS             complement(7092..7322)
                     /locus_tag="ACG8E6_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062231.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            complement(7319..7657)
                     /locus_tag="ACG8E6_001822"
     CDS             complement(7319..7657)
                     /locus_tag="ACG8E6_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062232.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            complement(7654..8052)
                     /locus_tag="ACG8E6_001823"
     CDS             complement(7654..8052)
                     /locus_tag="ACG8E6_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFS5062233.1"
                     /translation="MIPKFRAWDKRENTMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLG
                     HRNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            complement(8049..8267)
                     /locus_tag="ACG8E6_001824"
     CDS             complement(8049..8267)
                     /locus_tag="ACG8E6_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062234.1"
                     /translation="MDLYDIESGATVSVESDHLIISFADERQIIIWVVDDMLYPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSK"
     gene            complement(8254..8610)
                     /locus_tag="ACG8E6_001825"
     CDS             complement(8254..8610)
                     /locus_tag="ACG8E6_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFS5062235.1"
                     /translation="MDLITQYSDIILKKIMAKIQKDKKAKERAELVKLEMAETGVGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKAHSIQHGFI"
     gene            complement(8622..8765)
                     /locus_tag="ACG8E6_001826"
     CDS             complement(8622..8765)
                     /locus_tag="ACG8E6_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062236.1"
                     /translation="MKQANNMGFFMAKILLFLGLVGAAYLVYALLSQTDDKEDDNNDE
                     MKF"
     gene            complement(8749..9564)
                     /locus_tag="ACG8E6_001827"
     CDS             complement(8749..9564)
                     /locus_tag="ACG8E6_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFS5062237.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            complement(9575..9793)
                     /locus_tag="ACG8E6_001828"
     CDS             complement(9575..9793)
                     /locus_tag="ACG8E6_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HFS5062238.1"
                     /translation="MIGLTIFWCAIGGLLAFLGYYLICESRKGRKYTVTGIVLLIIGI
                     LVAVGAELNLYVNGTQEDLVRFMFWMKN"
     gene            complement(9790..10101)
                     /locus_tag="ACG8E6_001829"
     CDS             complement(9790..10101)
                     /locus_tag="ACG8E6_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFS5062239.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            complement(10098..10400)
                     /locus_tag="ACG8E6_001830"
     CDS             complement(10098..10400)
                     /locus_tag="ACG8E6_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062240.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVTEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEKK"
     gene            complement(10393..10557)
                     /locus_tag="ACG8E6_001831"
     CDS             complement(10393..10557)
                     /locus_tag="ACG8E6_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062241.1"
                     /translation="MDLKTFTAQIELMHQEALRQSASYEDKWLNTFHGGRESALDQVL
                     KLLKGERRDG"
     gene            complement(10562..10831)
                     /locus_tag="ACG8E6_001832"
     CDS             complement(10562..10831)
                     /locus_tag="ACG8E6_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062242.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGINNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLSELEQV"
     gene            complement(10843..11694)
                     /locus_tag="ACG8E6_001833"
     CDS             complement(10843..11694)
                     /locus_tag="ACG8E6_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFS5062243.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSEPKNASGAYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            complement(11701..12387)
                     /locus_tag="ACG8E6_001834"
     CDS             complement(11701..12387)
                     /locus_tag="ACG8E6_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS5062244.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(12393..13064)
                     /locus_tag="ACG8E6_001835"
     CDS             complement(12393..13064)
                     /locus_tag="ACG8E6_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS5062245.1"
                     /translation="MSENKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(13057..13398)
                     /locus_tag="ACG8E6_001836"
                     /pseudo
     CDS             complement(13057..13398)
                     /locus_tag="ACG8E6_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13576..14274)
                     /locus_tag="ACG8E6_001837"
     CDS             complement(13576..14274)
                     /locus_tag="ACG8E6_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFS5062246.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            14361..14831
                     /locus_tag="ACG8E6_001838"
     CDS             14361..14831
                     /locus_tag="ACG8E6_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062247.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(14874..15053)
                     /locus_tag="ACG8E6_001839"
     CDS             complement(14874..15053)
                     /locus_tag="ACG8E6_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062248.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(15040..15378)
                     /locus_tag="ACG8E6_001840"
     CDS             complement(15040..15378)
                     /locus_tag="ACG8E6_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFS5062249.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(15391..15576)
                     /locus_tag="ACG8E6_001841"
     CDS             complement(15391..15576)
                     /locus_tag="ACG8E6_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5062250.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALVGISGAYLSDILNGNRDG
                     KKAQEHIETVKKILGIQ"
     gene            15879..16253
                     /locus_tag="ACG8E6_001842"
     CDS             15879..16253
                     /locus_tag="ACG8E6_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5062251.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            16258..16686
                     /locus_tag="ACG8E6_001843"
     CDS             16258..16686
                     /locus_tag="ACG8E6_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS5062252.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            16736..17389
                     /locus_tag="ACG8E6_001844"
     CDS             16736..17389
                     /locus_tag="ACG8E6_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062253.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETNISKTKET
                     TEISSSTSKSTSKTDSSSTVTSSNQATAKPSPTVISSSQDTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNTEAQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            17526..18176
                     /locus_tag="ACG8E6_001845"
     CDS             17526..18176
                     /locus_tag="ACG8E6_001845"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFS5062254.1"
                     /translation="MKKNLILGFICTSILTLGACSSPSNNATNSETQKTINSLKKENE
                     ELKKQIASQSNDTEENSESIDNASKEKTLPVVGILEYTISSIKTEQVTNDEDNFTNAE
                     YNFSGIENFPEKYYRTTISYKLKNKATSPFSLSSYQASLIDGDGMEYSRTAGEYFLYD
                     ENSNSLVQPETTTSGVFYLLSKNKPNLSTFKLNASKQFTDDFTAEPIGEAGTTEYK"
     gene            18286..>19021
                     /locus_tag="ACG8E6_001846"
     CDS             18286..>19021
                     /locus_tag="ACG8E6_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5062255.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETS"
BASE COUNT         5566 a         3928 c         2867 g         6660 t
ORIGIN      
        1 gaacaaaaac aattagtgtt aatgctggtc ctaaaattga aataattagt ttagctcttt
       61 ctaaaaccaa attctttgaa actacactgt catttggaat aataatttct gatagtttta
      121 ttgccacaaa tgtaaaagct aaaatcccca acatgtaaaa tacccaagat atgttttttc
      181 cttttatcct catgaaagca cccctttgta aagaaaagta taccttaaaa ttcaatctca
      241 tccaacatat caaaagcatc atcgtaatca atctgttcat ctaattctgt agctttgtac
      301 aatgcgtgta caaatgcttg gaacccatca gttttacgct tgacttcctc tttttttaag
      361 aattgtttgc ctgcttgcgt ttcctttacg tacacattat tagtaaacca acgcattaac
      421 ggattatctc caaaaataaa tttgtgattt gcaaatccat cttctatacg aggagctaat
      481 aatggatgta aactagatgg tctttttagt cgaactactt caaatccttc tttttcaagc
      541 ataggcccta aaatatctgc cttatagtta tccacgacaa ttactctcac tccatattct
      601 tctctagctt gaataaacca atcaacaata tacttaggat ttaaagatgg ctcgtcaata
      661 actttcatca gccccatttt ttcccaagtt tttattggtg cccgtttttc actgccaacc
      721 aattgctctt tcaacgagta tccataatga acgtcgcaaa aatttttcat tgcaaacgta
      781 aatgatttaa acgcgtattc cgatttcttt cgatttttaa atagcaaacc gcaagtagca
      841 aaatctcgaa cactaccgaa atcgagagat ccaatcggta ccgtatctag ttcaaaaaat
      901 ggacgatttg ttgccattat ttcttctctt gaagcaacag agttttccat attgtcttca
      961 agaaaattca ttcgtttagt aacaaatgcc gagcgtccag aaggttcttt agctaacttt
     1021 ttgtactttt tcatgacttc gttaaacaga cgtttcccac gtttattcaa cggtggttgc
     1081 agtgccgggt ttgctttcgg ccaatttttg gggtcatcca tttcttcaat ggcatccagt
     1141 tcgcatatgt acggaaatac acccttgaac tctgacaaac catttaaaat atcttcacac
     1201 tctctatatt taatatcaaa gtaaccttca cgaacaaacc ccttagtacc aatgaaaaat
     1261 tgccgtccgt gatcaacttt ccccagtcca ccagaaaaca catctacaat atctgtgttt
     1321 tccatttcgt ggtattcgtc gtatattact gcaccttctc gaccgccatc ttgagagctt
     1381 gcattactag ttttgtactc aaaaacagac tgtgtctcta aaccggttag actgctttta
     1441 tacgcttcaa actcttcttt taaatcttca tttccttttt tattgattac tcgatagcat
     1501 tcttgaaaac ttctttttgc ttgtttctca ctattagcta caattgaaac atcatagtaa
     1561 tcaatcccgt gaagcgaact gacaaaataa tttgccaatg ttgaaataaa gccattttta
     1621 cccccgccac gccccatatt aataacaaat tcatcaaaaa caacttcgtc atcttcttta
     1681 taaaacaaaa aaatgaatgc agaaataaat ttctcccatt catctaactc aaaataccat
     1741 gcttgactaa actcgatata attatttatt tgctcctcgt cgaagtaata aatgtcgtcg
     1801 cgaggtaata tttctttttc tattagtttt aaaagttgta tccttttttc gttaaggatt
     1861 aactcaccag atttccattt acgaatatat tgatcaacgt gctggttaaa aagcattttt
     1921 atttcctacc gagtaactta cttttcttgt ccgctcttgt gccttgcggt aataaatcgg
     1981 tgagttgttt gacgatggct tgataagttt tatctcgatt atcataattt tcaactatag
     2041 gacgctttct atcataagca atttggttct cgctttgttt aaatttctcg tactccccgt
     2101 tttcaatgat gtccttccag ttatcatcca acaagacacg taaccttgct gcctgaatga
     2161 tcagcccttc aactaaatta agtttgtcat gaggaatttt atcgaatatc ttctgcagac
     2221 gtttcttttc tagctctacg cgtttttcca tgtcgtccat gcgatcattg agtaagaaat
     2281 gtaaactgca tccaatgacg atggaaattg agtttaacgt ttcgatacta ggggaatttt
     2341 tcccattttc aatacttcca taatacctac tagaaatacc acttttcgtt gctacatcag
     2401 cctgtgtcaa tcctttttca attctttttt gtttcattct cgtgccaacc aacttagaat
     2461 tcaaaccaat caccttcctt ctatatttac taaaggggcg gcggaggggg gaggggtaaa
     2521 ataaatacgt tgcatttgca gaattaaccc catccaccgg ttcccaaaaa ttttatttct
     2581 tctcaaaatc ttttgaccgg gggtatttca ctctatatat cttcgcttta tactgattta
     2641 tcagccgtct tgctcgttgc tttctacaat atgtgcaaat ggactcaagg tataatcaat
     2701 gtctacatta atattaaatg tccctgttac ttcattagcg ttacctatta gtctattgtt
     2761 gatagatata tcctctacag ttgttgtttc ttcaatgagc tgactcttaa atagaaccaa
     2821 cttatcaata gccttatcaa ccatagcgat tgcttctgct cgtttgacat catctctgtt
     2881 tgctgttttt cttaccacca ttcatcatcc cactttcttt tcttttgttg gccacgatat
     2941 ttcatacggt tgtgcctttt gttatggcaa tctttgcata aggttcttag gttgtttata
     3001 tctagagcta aatccggatg atactccagc tcctttatat gatccacttc aagaattgaa
     3061 tcatgttgag ttgttagtct accctctgct ttacaccatt ggcactcata atgatctcgc
     3121 tctaagcatt gacttctaag tttcctccac tcagttgatc catagaacct agctcttgct
     3181 tgtttagttg aaacatcaat catttacata tctctcttgt gcccaatgat ttttctatct
     3241 aagtacttgt tattatgtga atcataatat tcaatagtga tgtcgttagc tccttttggt
     3301 acttggcatt catattcttc cgtatgccaa tgataagtaa tatcgacaag acctatcggc
     3361 atctcatcca ttcgttgacc tttataataa acttctggta ctgagtcagt atctcttagt
     3421 ttgatttcta gaaggttcgt attacttcca tcatctaacg tcaattcagc aatcctcttt
     3481 ttgatctgtt gaattacttt tcttcctgtc tcgcctgtca ttggtactac cgtctcatat
     3541 ccagtaaact cgcaagtaaa gcgttcaata gcataagcgt tttgaagtgg cttagcacta
     3601 agaacagctg gattgttgct ataataacga tctactaata acttccctat aggaatggca
     3661 tctaaagctc tatcagtaaa cactaaaaca ttgggataat cttcttgaat tttgtaagct
     3721 agatttggtg tagttacaat gttataccct tccctgatat aatcttttaa tttcatagtt
     3781 tccctcctga aaacatccaa taattacttg ttaatgctat tgcatctttt cgacataaac
     3841 attcactaag gcttcttgca ctttgaatat cccttcaacg cctaatcctt ttacatctag
     3901 atctaaccta tctttcaaga acttagcatt gtgctctgct ctaattgttt gctcagcgat
     3961 gaaataattt agtgccgcta cttcatccat ctttaacccc accaaactaa tgatgttcat
     4021 gaataagtta gctagttcgt ccatatcttt ttctgctctt atcttttcaa tcaacttgat
     4081 gtaatcatag ttatcattca tttgatgtac ctctcaatgt tttgttgaat atattcgtct
     4141 ttccaatatc catggccaca gtaacgaaga ttgtacttat cgatctcatt tggtgtagct
     4201 tctctggtca tttcaatgat ggagtacttc tttttgattt gtacagaacg cacaacacgc
     4261 actgaacaat catcaatggt tcgaggatat tcattagtta gcgatatata ccagtagttt
     4321 ctcattatgt atcactcttt ctgttggtta ctggcagaaa aggttcgcat cataaattcc
     4381 atagcctgtc cttcattaaa cccttgtaga ataagctggt catagaaata cttagcttgt
     4441 tttgcgatta acgctaagct tttttgagct tcatagaatg ttgcctctgc agtttgattt
     4501 gattcattca atttaaataa ctcgcttaat tgtttttcat tcatagataa aactcctttc
     4561 aaaataaaaa gaccactcaa agagtgatct aatatgtaaa aactacacct cataaacgag
     4621 atgcagttat agttctatca agcaacctac accgattcca tcgattacta tcgacctcgc
     4681 cttgctcgtg tacttggagc gcccatttcc aaccctcggt tgccaaagtc actggcaatg
     4741 aatcgaacat tgcatggttg ccgaagcatt gacctagcac gcatgcttag cgtctaccct
     4801 ttccgccaca gtgacaaatt aatattgtga aaataaatac taagcgtata attttattta
     4861 tcagcgagtg gtccgctgaa ataaattata ggtggtgaat atatgaaaat aactgctgct
     4921 attaccaaag aacagggcgt aacttttaca gttgttgttg tgaaaaacgg tgtgatctct
     4981 tcttctcata gagaacaagt tagaagtagc atcccaagaa atttcccaag acctattatc
     5041 ttagctgaac agtcgcatgg gaaaatgaag tatcacggta gaacagatat cgtaaaattt
     5101 ctttctaatg taccttatca agctttgcct tggaaggatt atactttata ggttctgcta
     5161 ccggattgtt gatatcccct tctggtaaat caatgttcca atttgctttt acaaaaaaac
     5221 tagaattttc actttcagct ttattcacaa tagcaatttc atctaaagtt tcttgtaact
     5281 gttcgacttg gtcactagcc ttgttgagca gttcttttag ttcagtcaaa tctaatttga
     5341 cagttacttt gatttcttta tccattcgtt ttccctccaa tacataaatt aatagacagc
     5401 aacggatgat agataataag aacaatttag aaggagttaa aattcacatc cttattctta
     5461 atatttccgc tgctgtctat cgaagcttaa ttgtgaaaca ataataaaac gatgttcctt
     5521 ttattattat tttgtcttag acctatcact aatctttcga cactatcata atatcacgtt
     5581 aaaccactca aaaaccctac actatcccta caaaaaccct acaaaatcaa cgatactgaa
     5641 ctaatacgcc ttttttgtat gcttcagcaa attcgatcag cgcgatagat ttcagcttct
     5701 ctacattctt ctctccgtat cctcgtatca attgccctat ttcataatta gagtgcttgt
     5761 ttacgtcaca aaagctataa tagagtatct gacggctaat cagactaaga gccatcaaag
     5821 ccgctagaat cgcatctctc tctgcttcta tatccatcat ctgaataatc gcgtcctctg
     5881 ccttattgcc gtgcttcggt gccttcggca tatccgtaat aatcggcgac ttaatatcta
     5941 tcaaagagcg acctgccatc cgctctaagc gtcgaaaatt cttcagcaca tctctcgcat
     6001 tgcatcttgt ctgtttgaaa tctacctctc gtaacaattg catcaagtca aaccgctcct
     6061 ttatgtgata taataaactt gttgggttta ttacatcagt cggagcgatc cggctttttt
     6121 tatttgccga gttacacttc tcgacttagc atgctggtgt gtcaaatact tcaatatatg
     6181 cttcttctgt ttttattgtc tttttcattc cgccattcct caacctccgc aattttgtta
     6241 taattaactg tcaggtaaat tataattgtg cgtttagcta aggggctaaa cgttttttta
     6301 ttttattttt gatgttcttt cataaaacga tcacattctt ctttgctgat acgaataacg
     6361 ccgttaacga tacccatttt caaaccagct ggtacgtact tatccatcat cgtgttgtaa
     6421 cttacaccaa gatattttgc tgcttgcatt ttgttcatat atttcggaaa ttggtatttc
     6481 ttttctagca aagcaatcaa ttcatcttgt tttttctcga ccaattctgc aattcgttca
     6541 tccatttctt gaaatgctgg ttcttcaaac gttaccttgt caatttttaa ttccattgtt
     6601 tataacctcc atatccacca atctcaccac tgctaaattc tctttgcttt tcgctaacca
     6661 cttatcgcat tccattgtgt tttcaatacg aatgatcgct gagtgattat agagatgctc
     6721 tacatatcca cgaaatggat agatgaaccc ttctgcttcg cagcgaacca tgtcaccgac
     6781 tttaatttct gattcctctt gctttatagg attcttagtt ggtagatcca tcatcaaacc
     6841 accaattcca taagcatcag tgtaaaatcc gtcttttagt ttcatctttc tgcctcccat
     6901 ttacgatcat catttaatat cgaaatccca aacttacgaa tagtctcact cgcatcagca
     6961 acaaactgac ttgccacttt atatgtttct tctgctgaaa ttccatattc tttttcaaac
     7021 tttgtcttta gtacattcag ttcctgtttt cttagttttg ctactctgcg gtgtctggtg
     7081 ttcattgtca atcaactccc taatctgaaa gtgtcgttta tacttgatcg aaattctttc
     7141 aagtggttct ctaccacaga atcagtcacg ttaaaacgat caattaatac tggagctgcc
     7201 atatctttca aataactttg tctgatgact aattcagtac catcaggaag ttttttatta
     7261 acctcccgac cattgataat tgcttgaatg cccgcttcac ttagtggtat ttcgtatttc
     7321 attccgcttc ctccttcaac cataaatttg gaaaatcatc ggcatatatc tctattgcgc
     7381 caaataactt gatcgctgat tttgattttc catgcatgtc aggactgatt aaacgatcat
     7441 aataatcctg actgattgtt tttcccttgt actgataacg tgtagccgca gtgtaatata
     7501 ctgctgtctt gctcagataa agatattttg ctaaatcagc cgatatccgt aacaaaatga
     7561 ttctattctt gcttttaatt ccacaattta gcaatgctgg gacattccca ttttcatctt
     7621 ttaaagaatc gatgtagtca gtagctaaga taatcactct acctccaata actcgctatt
     7681 ctcgtatata tttccgatga cttcatgagc agttgctaca tcataacttt cgccagtatt
     7741 cctatggcct aatctataat cacttttctt tagtacaact acaaagccgg cttcttgttg
     7801 aagatacgca acatatccaa tcgctccttt tgatgtaatt actacatccc cttcaaatat
     7861 ctccacacca ttcttatctt tcattcctgt ggattgcatg aggtcgatgt ttcgcacgtg
     7921 atatgatttc aattcggtag gatttatcca atattcaata aagtttgttt tgccgttttt
     7981 agtaaaatgt aagacagcta catctctcat tgtgttttct cgcttatccc acgctctaaa
     8041 cttcggtatc atttgctgtc ctcctcgtat ttttcaatca attccattac ttttttcact
     8101 atttcaaact caaccgcttt tgattcttca aaatcatgta caatttctgg atatagcata
     8161 tcgtcaacta cccatatgat aatttgacgt tcgtcagcaa aacttatgat taaatgatca
     8221 gactccacag atactgttgc tcctgattcg atatcatata aatccatgct gaattgaatg
     8281 agctttttta tcattcgctg tcctcctcaa atactcttct aatatttctt tatacttctc
     8341 tacaaatttg aaacgatctt gatgaagttt cttgctccaa tttgtttgcc gatccagctc
     8401 acgcatctga tcgaaccctt tttgaatttc gttgtaataa aattcaatgt ttgctgctgc
     8461 tttccaatgc ctgctacttc gcactcctac tcctgtttca gccatttcca acttaactaa
     8521 ttccgctcgt tcttttgctt ttttatcttt ctgaatcttt gccatgattt tcttgaggat
     8581 gatatcactg tattgtgtaa tgagatccat tatttctcct cctaaaattt catttcatcg
     8641 ttgttgtcat cttctttgtc atcagtttgg gaaaggagcg catacacgag atatgctgct
     8701 cctactaaac ctaagaacaa gagaatttta gccataaaga atcccatatt atttgcctgc
     8761 ttcatccgta actacagtat ctgctccatt tactgttacc catccatgct ttttgcgagc
     8821 ttgagcttct tcataacgaa tcaaattgtc tgttactgat tcggcgactt tgcgattaga
     8881 ttctgcctct gcttctgcag cttttgtttt cttgtaagct tcactatcag cttgagtttt
     8941 tgcagtttct gcatctagct tcgctttttc attttcttga ccagctcgaa tgatcgcatc
     9001 gattgatttt tgtgtttctt tatcgacatc tggaacacca agtgttacgt cttcgacttc
     9061 aaacccttta gattcaactg atttagcaaa gttcgttaat acctcagctt caactttaga
     9121 ggaatctcct gaaaggacat caagcagact atatttcgca taaacttcac gagcgacctt
     9181 ttgaagctta gattttaacc atccactttc gatatcttcc gaagtgatat tcccaaattc
     9241 cttgtacatt tttgctgctt tagttgaatc aactttgtag tcatatttga tatcaatcgt
     9301 tgtctttttg ccgtcgcttg tagatactga aatatttttt gattggatag tttgcaagcg
     9361 aattggatat tggatcactt tgtcaatccc tacaaatttc acaccttgcg tcagtgcttc
     9421 atctttgata ccgccgttca ttgaatagcg cacacccaca tatccattat cgattttttc
     9481 gaagaactta aatcctccga taactccaat acctacgatt actgctcctg ctacacctaa
     9541 tttgattaat ttgttttcgt tcattttttt cctcctaatt cttcatccaa aacataaatc
     9601 gcaccaaatc ttcttgtgta ccatttacat aaagattcag ttctgctcca actgcaacta
     9661 gaattccgat aattaacagg acaatacctg tcactgtata ttttcttcct tttctacttt
     9721 cacagatgag gtagtatcct agaaatgcta gtaaaccacc aatcgcacac caaaatattg
     9781 ttaaacctat catttgctgt cctccaaatc actcgacttc acgaatacac catctaccat
     9841 ttttcctgtg cgccctttga tttcgttgta tgccatttct aaacactctt gtacgtttgt
     9901 ccctttttgc atggaaagga taatcaacgt gacgattacg tctcctacgc tatctttaaa
     9961 tagctcatca ttacttcttg ccattgctga agcgatttcc ccgaattcct cagctacttt
    10021 caaaaactgt gcttttggat ctgcttgatc cagtcccttg tctttagccc actgctctac
    10081 ttttgtgatt agttcgttca ttttttctcc tccacatacc taaattgtcg tccttttgaa
    10141 tcaatccata agctccttgc tctatcccag atgatgtttt tgctcagacc agtaatttca
    10201 gataactgtt cagcagtacc tgttactaga attcgatcac catgccaaat tgcaatcttt
    10261 ctcggcgttc tccgtttggg cttttcagtc cacattgatt taccgagctt ttggacttct
    10321 gtaactattt ctttgtcttc ttgccaattc tcagaatgtg tcagttcgat gattcgtttc
    10381 attgccgctt tcttatccat cccgacgttc ccctttcaat aatttgagta cttgatcaag
    10441 tgcgctctca cgtccaccat ggaacgtgtt gagccacttg tcttcgtacg aggcgctttg
    10501 tcttaaagct tcttgatgca ttagttcgat ctgtgctgta aatgttttta gatccatctg
    10561 attacacctg ctcaagttca ctaagatgtt tttgcaatcc tttaacgcaa tcaacaaata
    10621 gtaattttgt ataagctaaa tttcttaatt gtgttgtatc gatatagagt gcgaaatagt
    10681 atctgagttt actccaactc gaacgatcat tattaattcc ttcgattcca gcttcttcga
    10741 gttgatcata tacgtctctc aggatttcta tttcctcacc agttttatac tttgctattt
    10801 cattaattag ttctagataa tcgattttca attttccacc tcttaaaatg gtgcttttga
    10861 ttgtctatta gctcgttcta gcgctttttt cttgagatag gcttcttggt cgattgccca
    10921 ttcaggaagc ttctctcgtc ttcctgtgcg cttgtatgca ccacttgcat tcttaggctc
    10981 acttttttct ttccttgccc aacttcgaat agttgccaaa tagtttttat aagtcttacc
    11041 agatgattca caatactcag atagccgttc tattcgttct tgatagtcat tagggaattc
    11101 tattttgagt ttctccatct gctcatctga caaaagaaca tttttatact ctccgtattt
    11161 atgacggacg ggcttagcct tcgatttttt cgaaggcgtt acattctcta tatctttctc
    11221 taactctata tctttctcta actctatctc taactctggt gtagttttgt ccggacattt
    11281 gtccgacatt tgtccacttg ttattaaatt ctgttttgct tcctcaattt cttttctgta
    11341 ttttctcttc ctatcagctt cagttgaaga tttcccaata aaactttgta tatcagacat
    11401 ataaattgct ccgttatcta atacctcaat aagttgtaaa tcacgaaata tttgtactgc
    11461 tttttctaca actcccacag agtgtcttgt aatagtcgca agcattgtag aattgaatgg
    11521 aatcctgtca ttaaacatta gtttgccttc atgtttcaga cttcttaaat aaagtttgag
    11581 aagaatatta gaataaatgt atccatctgg catactttct aaaagaacca tctcgtcgct
    11641 atcgaaaaag ttctctttta gttttaaata gtagtatcgt ttgttgtcag ccaatatttt
    11701 ttatcctcct attctaagtt tcttaattgt ttcctggttt aacttgatcc ctttgatttg
    11761 atatttattt ttgaaattga tcacacctat cttgtgtttc tccgtgtgat ggattctgca
    11821 gagtgctgca aatgtgtact ctgaatgatc aacttctttg cgctttcgtc ttcctagcgc
    11881 tttgtcaaag tgatcgatat cagctcctgt tttgccacag atgcaacaga ctctttttgt
    11941 gatgcatttg tagaagtaat attcttgatt cgctggtaaa atctcatagc cttctttgaa
    12001 aggaatatga tgttcaaaga tgaaatctaa gatgatattt gctaagacgt tagcatcact
    12061 cacagtcgta ttcgattcat ctttgaggct tattttgcgc cctgtgacac cttcaaagcg
    12121 gaagtaaaag aattccttcc agaagtccgt tggcatgcct gtatcgatga aaatatcgcc
    12181 tatgagtgca tagatgaagt ttcgttgctg tactgtgaaa cgtctaggat caataaaacg
    12241 aatttcaata actcgatcac catcgtagcc gtcatacatc gtctttagtc gatcaatgtt
    12301 cacttcctca ttgatggttg cacctatgtc ttttcctttg aactttttca gaaccgctga
    12361 atatgaatcg attaatggtt taaacactca tatcacttct tatctaattc ttttctctta
    12421 gctgctattg ctcgctccat caaggcacat tgctcatagc ttaactgttc aatagtttca
    12481 acgttatcag ctaagagccc taatttatct gtctgctcat taacatattc gattaaggtt
    12541 ttggtcatat ctttacccat ctgctcattg aaagcttcta gaatcgtctc tagcatgttt
    12601 aatttcttcg tatcgattct aggtggtgtt ggaatatctt ccccttgaaa tacatataat
    12661 cccagtccgt gtagagccaa tgctttcaca aagcatcgct tcaatgagtt attgatttgc
    12721 attgcatttg gtttaacaac tggttggttt cgataatcta aaacaggaaa taactcggtt
    12781 tccgtgtgtc ctttaaccgt tactgagaca gatacataag tccctgtttc atccataaga
    12841 aaaggtttgt attcctcaac aagaaagtct tgatgagttc cagaaacaac tctgtagtgt
    12901 ttgtactcat taatagttac tgttgcctgt ggatcattct ttttcataat ctcccatgcg
    12961 tgagcccaag ataaataatc aaaatttcct tttttcttga gaattttatt taacttgcga
    13021 ctaaaaagtt tttcaaaatt cgttgtccct ttgttttcac tcatcaaatt ctgcctccaa
    13081 ttcagcaatg tatttcttac ctggtccgta ataagagata tcgatcaagt tatccctttc
    13141 gtactcttct aatgcatcaa tcaagccatc ttcgatgacg taaatatatt caggtttatt
    13201 cgaatgcttc gataaatgga taagataaac atgatctcaa atagttacaa ggttacctaa
    13261 atcatcttga tcacaagcta gttcttcatt cgtcaaaaga tttcgcctga tttttcgacc
    13321 acttgtttcc tcaattttcg gcttgcccca atcaggatca atcaaatatt gatctagagt
    13381 ggaaagttct ttttccatat gctaaaatct ccttatgatg tgttttcttt gtgactcttt
    13441 gcttgccggc ggagtcactt ttttatttgt tgccatgctt tttgcttgtc aatatgttgt
    13501 tggcttagga tgattggttt atagtatttc caccagcaat tagcaattgc cgtccctatt
    13561 cttagcgctt cagctctatt cattgtcatc accaaaaagt ctctgttgtc tgttcagttg
    13621 atcaatttcc atacggatcg cagtttctgg caaccacatt tcaataaatg aaacagcatc
    13681 atcgaatctc ttacgaggta actcgccata tcttgggatt gaaaaggtac gtttaaattc
    13741 agaccaaaat tttgagaata cttttttgct gatttcttca taagctcggc tttcttttcc
    13801 ccctagaact cccataactt tcatatttcc tttttgctta atttcaaact cttgttgtcc
    13861 gctaattcgc atagtatctt taagcatgga aacatctttt ttaacatctt tcatttcttc
    13921 taattgatag atcatcatat cttcaattgt ttgaggaaca gtattcttgc gaataacatc
    13981 ttccatttcg ttgaatgctt caatgtattt ttgtttgaag tagatagctt tctttcctgt
    14041 aaaccccata gccagcaaga aaaatccatc tctactaatg aagaaaactc gtcgatttct
    14101 gccatatgaa tctggttcat taccttctac aaacatctgt ccaaaattgg acacatcttt
    14161 ttttagtgca tcaatatctc ttaaaacatg ttgatgtttt ttctcgaagc tttctgccac
    14221 ttgtaagctc gtagtcacag cttctttatt tttcaaaatt actaattctt gcattatttc
    14281 ttctctcctt tttggtataa tttagttaaa aactggtggt gtttattttg aatttttttc
    14341 acttaacaat ttttcaatgg atgtcaataa taagttttat attgagtacg tttttaacag
    14401 ggataaaaat aaaaggaatg cgccctcaac tagaagttga acttaatgct tcttacttcg
    14461 ctgcagatat gatttataca aaagtaatca tttctaacta ttcaacagaa ccagctatgt
    14521 tagttaattt agaactttca tccgctaact taaaccgcag atggtcagct actccattta
    14581 agaaattaat tgctaaagga ggatcagtcg atgataatag aatatactct gaagctgtac
    14641 cgctaaatat tcctcctaaa agtgctatat cttgttacct tgcatttgaa gtagggaaaa
    14701 ttaattttaa taaactcctt aatcaccaaa caaaaatgat atttactcta aatagaaccc
    14761 aaatccacaa aattgttgat atcaaaaata caaacttccc aatagagaaa ttagtgaaag
    14821 aattaaactg agtaatagta atatacattc tagtttctct tttctattca tttttagtca
    14881 gccccctcgg ttggcttttt cgctctgtac tcagcttcag cttcatcaag ccccataaaa
    14941 atccaaacca tgtaaacaat cgttcctatc aacgcttgcc tgcttcccca aagtcctaaa
    15001 gcgtagatga ttagcggtgc gctgaacact agcgctctat taaattttcc catccgcttg
    15061 cctccttaag atttccgaaa aatttgtttc taaaaattca agtgttttac ttcttaaaaa
    15121 tagatatgtg tctcttcctt caactggata atagacaaat ccatttttgt ttttctcgat
    15181 atcgataata tttctatatc ttgggttctt taaaactcta gaagtaaacc aatcatattt
    15241 tctgttaatc cgttctagca cttctggcaa cgtcatccat ctaccagtat catcagcttt
    15301 ttttaactcc tcgtaatcca cttgagagat aattacatag ccttctggaa ctgggatttt
    15361 cgcttctaga tattgcatta gctgttcctc ctattgaata cctaatattt tttttacagt
    15421 ttctatgtgt tcttgagctt ttttaccatc acgattgccg tttaggatat ctgataaata
    15481 agctcctgaa ataccaacaa gcgcagctag ttctttgaaa gtcattcttc tttttctcat
    15541 ctccgctcga atttttaagt ctaaattctc agacataaaa atagctcctt tctaaaaaat
    15601 aatttgtaag ctaaaaaatt agctaaatcg ttgacaccta ttagctttta agctattatg
    15661 aatacatagt taaataagac ttataaaaag cctctaaaat aacatttcta agtttggcga
    15721 ccgagagaat gttttaaatt agtagagttt tttgttgctc ttatttagct aacaatttag
    15781 cttacgaatt aaatatacta gcttaaaagc taattgtcaa ccaaaaatat aacttttaag
    15841 ctatttattt tcttttcagc tttgaaaggt tgataataat gagtttagtt actaagatta
    15901 aagaattagc agacgaaaag catgtgacta tagcagaagt agaaagacag gtgggcatct
    15961 ctaatggaca aataagaaga tgggataaag cctcgccaaa atctgagaac ttaaagaaag
    16021 ttgctgatta ttttggtgtc acaactgatt atttattggg aaataataat gttcccaaat
    16081 gggctacaaa agaggaagtg gttgaacttg ataaactact agactcaaat gttaatatgt
    16141 cttatggtgg ggaaacattg acacccgaac aaatacagcg cgtaaaagat atcctgatag
    16201 cgactttctg ggatattgtg aaagaagaca aagaaaaagg caaaaagatg tgagcttatg
    16261 gagatggata cgattaattt agtcgaggag ttgaagcgga aataccagtc cgctaatcct
    16321 ttttatattt gtgaaaagat gggcattaaa attcaatacg ttccttttat cgagaatccc
    16381 aaggggcagt ttcaagaaat tagagatcgt gcgataatct ttttaaatga tgaactgcga
    16441 gactctgagg aaagattcta catttgcgct cacgaattag gtcacgctat ttttcatcgt
    16501 ggcttatcca gctattatgt atcaacaaga acatctagaa gcaaatctga aagcgaagct
    16561 aattgctttg ctgctaatct cattgtttct ctttataaag aagataatga tcaatatcct
    16621 aaaaaaatcg aggaattaaa gaatctttac ggtcttccag aaagcgctta tcgttttctt
    16681 atataaaaaa gcccgtgtta gcacacatat tacaacgaga aagaggaatt ataaaatgaa
    16741 aaaagttagc gttatgttgt tgttaagtac tgctctgcta ctttcagctt gttcaaataa
    16801 taaaaaagct gaatcaacag atgccacttc taaccaagaa acaaatataa gtaaaacaaa
    16861 agaaacaact gaaatcagtt catctactag caaatctaca tctaaaacag attctagttc
    16921 aacagttaca agctccaacc aagctacggc gaaacctagc ccaacagtta taagctccag
    16981 tcaggataca atccaaaccg cacctcaaga agaaacatat gaacagatga aacaacgcac
    17041 tttacagtca actccagctg atcgtgcaaa ttggtccaac aaagagtggg aagctttcgg
    17101 cgtggccctt tatgaaaatg gattgactac agatgatgct ggcaatatta tcagtcaaga
    17161 tcagaaagaa caacaagctg catctcaaca aaatacagaa gcccaacaaa caagcgctca
    17221 acaagacgct gacactttat cacttactga ttttgttaac aaatacggga tgtcgcctgt
    17281 tgcatggaaa gtacagaatg gaatgtctga agaagaagca ttgcgtacaa cacagcaaaa
    17341 aacttccggt gaagttcaat taggattttc taaatacgga atacaataat atatttttgt
    17401 gtcccactat cttgcctata acctctaaaa agttataaag aaaaaagccc gtgctgcaac
    17461 acggactaaa atctcatttc taagattcta cataaaaata atatcataga agtgaggaat
    17521 taaaaatgaa aaaaaactta atattaggat ttatttgtac tagtatttta actttgggtg
    17581 catgtagttc gccttcaaat aatgcaacta atagtgaaac tcaaaaaaca atcaatagtt
    17641 taaaaaaaga aaatgaagag cttaaaaaac aaatagcatc gcagtcaaat gacactgaag
    17701 aaaatagcga atctatcgac aatgcttcta aggaaaaaac cttgccagtt gttggtatcc
    17761 tagaatatac aatttcttct ataaaaactg aacaggtaac aaatgacgaa gataatttta
    17821 caaatgccga atataacttt agtggaatag aaaattttcc agaaaaatac tatcgtacta
    17881 ctatttctta caagttaaaa aataaagcaa catcaccttt ctctttgtct tcgtatcagg
    17941 catctttaat agatggagac ggtatggaat atagtagaac agctggagaa tatttcttat
    18001 atgacgaaaa ttctaattct ttagttcaac ctgaaacgac aacatctggg gttttctatt
    18061 tactttctaa aaataaacca aatttaagta catttaaact taatgcgtct aaacaattta
    18121 ctgatgattt tacagctgag ccgattggcg aagcaggaac aacagagtat aaataaaaaa
    18181 cacgccccct ccctcgccaa agtttgtgga cgcgttaaaa acaaacctac acaataggct
    18241 tctttacagt gcctattgta tcagagaaag agagtagatt caattatggc aagattagtc
    18301 aaacgtggaa atagttggca atacgaaatt tcatacaaaa aagatgacgg aaaatacacg
    18361 aagataagaa aatcaggatt caagacaaaa ggcgaagcaa aagatgccgc caacgaatta
    18421 gaatataacc tgaataaagg tcttaaaggg gatcgcaaaa atctattatt atcagattac
    18481 tttgaagatt ggatgcaact ctacaaagaa ggaacagtat ctcctatcac ttatagaaaa
    18541 tacgaagata cgttaatgaa tataaagaaa tatatgccag cagtattgat ttctgattta
    18601 gatagagttg gatatcaacg ctttttaaat aagtatgcga aagaccatgt aaaatccacc
    18661 gttattaagt ttaataacca tattagagca tcgttgaaag atgccgtaga agaaggatta
    18721 attccgtttg atccaactag aaaagcagta atcaaaggaa aagattcatt gaagccaaaa
    18781 gaagataaat atttagatta tgatcaattt aaatctttaa tgaaactcgt agaagaaaac
    18841 ctttctgcac agtactcttc tcctatgctc gtgttagttg ctggtgctac tggaatgcga
    18901 tttgctgaac ttctaggatt aacatgggaa gatatcgatt tcgaagatca aatcatcact
    18961 attaataaaa catggaatta taaattaaat gaatggggaa aaacaaaaaa cgagacttca
    19021 a
//
LOCUS       DAZUMM010000055        17731 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000055 DAZUMM010000000
VERSION     DAZUMM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.055"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..842
                     /locus_tag="ACG8E6_001847"
     CDS             30..842
                     /locus_tag="ACG8E6_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFS5062256.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            839..1639
                     /locus_tag="ACG8E6_001848"
     CDS             839..1639
                     /locus_tag="ACG8E6_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS5062257.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1800..3068
                     /gene="rseP"
                     /locus_tag="ACG8E6_001849"
     CDS             1800..3068
                     /gene="rseP"
                     /locus_tag="ACG8E6_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFS5062258.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3136..4845
                     /locus_tag="ACG8E6_001850"
     CDS             3136..4845
                     /locus_tag="ACG8E6_001850"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFS5062259.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5057..9409
                     /locus_tag="ACG8E6_001851"
     CDS             5057..9409
                     /locus_tag="ACG8E6_001851"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFS5062260.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9553..10026
                     /gene="rimP"
                     /locus_tag="ACG8E6_001852"
     CDS             9553..10026
                     /gene="rimP"
                     /locus_tag="ACG8E6_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFS5062261.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10050..11225
                     /gene="nusA"
                     /locus_tag="ACG8E6_001853"
     CDS             10050..11225
                     /gene="nusA"
                     /locus_tag="ACG8E6_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFS5062262.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11247..11540
                     /locus_tag="ACG8E6_001854"
     CDS             11247..11540
                     /locus_tag="ACG8E6_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFS5062263.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11537..11848
                     /locus_tag="ACG8E6_001855"
     CDS             11537..11848
                     /locus_tag="ACG8E6_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFS5062264.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11861..14167
                     /gene="infB"
                     /locus_tag="ACG8E6_001856"
     CDS             11861..14167
                     /gene="infB"
                     /locus_tag="ACG8E6_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFS5062265.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14194..14541
                     /gene="rbfA"
                     /locus_tag="ACG8E6_001857"
     CDS             14194..14541
                     /gene="rbfA"
                     /locus_tag="ACG8E6_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFS5062266.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14649..15153)
                     /locus_tag="ACG8E6_001858"
                     /pseudo
     CDS             complement(14649..15153)
                     /locus_tag="ACG8E6_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15318..17681
                     /locus_tag="ACG8E6_001859"
     CDS             15318..17681
                     /locus_tag="ACG8E6_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFS5062267.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6043 a         3147 c         3908 g         4633 t
ORIGIN      
        1 ggaatctttt aagtatagga ggaaaaagaa tgttacgttt ttttccgcaa aaaaacaaat
       61 atattcaaga aaaaagcgaa tttcaattta ataccgatgg accaattcct cagcacattg
      121 ccatcatcat ggacggcaat ggtcgttggg cgcaaaaccg tcgtttgcca cgagttgctg
      181 gccataaaga aggcatggaa acagtaaaga aagtcacaaa aaaagcatca aggcttggag
      241 tgaaagtgtt gacgctttat gccttttcga ctgaaaactg gaagcgacca aaagatgaag
      301 taagttttct catgcaacta cctgtcgact tctttgatac gttcgtacca gaattgatta
      361 aagaaaatgt caaagttcat gtcatgggtt atgaaaacgt attgccagaa catacgcaag
      421 atgctgtaag acgtgcaatt gagcaaacaa aaaacaatac tggaatggta ttgaattttg
      481 ctttgaatta tggaagtaga gcagagatcg tgactgctgt ccaagaaatt gccgaagaag
      541 tagcaaaagg agaaatacac gcagaagaaa tcgatgatga attgattgct aagcatttaa
      601 tgacaggttt cttgcctaaa gagttacaag atcctgagct gatgatccgt acaagtggag
      661 aagagcgaat tagtaatttt ctgttatggc agatcgcata tagtgagctg tattttacaa
      721 aagcgctttg gcctgatttt gatggtgcgc atcttgaaga agcaatcgcc tcttatcaaa
      781 atcgagaccg tcgattcggc ggtgtaaaaa aaacagaaga tgagaaaggg gatcagtcat
      841 gaaacaacgt gtgatcacag cagctgtggc gctagtcttg tttattccga ttatatggat
      901 cggaggcata gcggttgagc ttacagcagc aatcttagcg gtagtaggag tttacgagct
      961 gttccggatg aaaggattga cactcttaag ttttgaagga gttttatccg ccgttggtgc
     1021 agtgttactt gttttgccaa aagaaagatg gtttttcttt cttccagaga atgtaagtac
     1081 atttatgtta ttttatctta tagttatgat tcttttagga acatcagtta tttctaaaaa
     1141 tacgtatacg attgatgaag caggttttcc tgtgcttgtc agcttgtatg tgggaatagg
     1201 tttccagaat tttgtcaatg cacgaacaga aagcttggct gttttgatat ttggtctatt
     1261 tattgtttgg gcaactgata tcggagcata tatggttggt cgacgttatg gtcagcataa
     1321 gttaagtcct gatatttcgc ccaacaaaac gattgaaggt gcgctcggtg gtatcggtag
     1381 tgctgttgta gtcgcatttc tttatttatt gctataccca gcaaaagaac tattcaatca
     1441 tggtatgttt gtcatgcttc tgatgacgat tattttttca gcagtcggac aatttggtga
     1501 tttggtcgaa tctgcaatca aacgtcatta tgatgtgaaa gactcgggaa atatcttacc
     1561 aggccatggt ggaatcttag accgtttcga cagtttgctg tttgtatttc ctatcatgca
     1621 tttatttggc attatttagt aagtatacct gcgtttcttt gtgaatttca taaaagacgc
     1681 aggtttttat ttttcattat attgatacta ttggtgtctt tggtatacta tggtaaaatt
     1741 atagtgtttt atcgttgttt tataaaaaac acaaaaaaca agtaaaggag aaaaaagcta
     1801 tgaaaacgat tctgacattt atcatcgttt ttggtatatt agtgattgtt catgagtttg
     1861 gtcatttctt ctttgcgaaa cgatcaggaa tcctcgtccg cgaatttgct atcggtatgg
     1921 gtccgaaaat ttatgggcat caagcaaaag acgggacaac atatacgctt cgcctattac
     1981 ctattggcgg ttatgttcga atggcaggca atggggatga tgaaacagaa atggcaccag
     2041 gaatgcctct ttctctttta ctaaattctg acggcatcgt agagaagata aatttaagta
     2101 agaaaatcca attgacaaat gctatcccga tggaattgag ccgttatgat ttagaagatg
     2161 agctgactat cactggttat gtgaatggag acgaaacaga agtagtaacc taccctgttg
     2221 atcacgatgc gacgattatt gaaaacgacg gaacagaaat cagaattgcg ccaaaagacg
     2281 tacagttcca atcggctaaa ttgtggcagc gtatgctgac caactttgct ggacccatga
     2341 ataactttat tttggcaatc gtgttattca tcattctggc atttatgcaa ggtggtgttc
     2401 aagtgacgaa cactaatcgc gtaggagaga tcatgcctaa tggagctgcg gcagaagcag
     2461 gcttaaaaga aaatgatgag gtagtcagtg tggacggaaa agaaatccat tcatggaatg
     2521 atctgacaac agtcattacg aaaaatccag gtaaaacatt agactttaag atcgaacgag
     2581 aaggtcaggt gcaatctgtc gacgtgacac cgaaatctgt agagtcaaat ggcgaaaaag
     2641 ttggccaact tggtataaaa gccccaatga atacaggttt tatggataag atcattggag
     2701 ggacgcgtca agctttcagc ggttcattgg aaattttcaa agccttagga tcactattta
     2761 caggtttcag tttagataaa ttaggcggac ctgtcatgat gtaccaattg tcttcagaag
     2821 cagcaaatca aggtgtaaca actgttatta gtttgatggc gcttctttca atgaatctcg
     2881 gaatcgtcaa tctgcttccg attcctgcct tagatggcgg gaaattagtc ttgaatattt
     2941 ttgaagggat tcgcgggaaa ccattaagtc aagaaaaaga aggaatattg acattagctg
     3001 gctttggatt tttaatgttg ttgatggtgc tagttacatg gaacgatatt caacgattct
     3061 ttttctagaa aaagttgaca aatagggtat aataacaaac gataactatt ttgaggaaat
     3121 aaaggagtat atagaatgag acaatcaaaa atgttgattc caacattacg cgaagttcca
     3181 aatgatgcag aagtattaag ccatcaaatc ttgctaagag ctggatatat ccgccaagta
     3241 tcagcgggta tttattcata tctgccatta gcaaatcgtg tattggaaaa actgaaacgc
     3301 attatgcggg aagaatttga aaaaatcgga gcggtagaaa tgctgatgcc tgccatcttg
     3361 ccagcagaac tttgggaaga atccgggcgt tacgaaacat atggaccaaa cttgtatcgt
     3421 ttcaaagacc gcaatgatcg aaaaatgatc cttggaccaa ctcacgaaga aacttttact
     3481 gatttgatgc gtaatgaaat caattcatat aagaaattac ctttaaatct ttatcaaatc
     3541 caacctaaat atcgtgacga aaaacgacct cgcttcggtt tgcttcgcgg aagagagttt
     3601 atcatgaaag atgcatattc tttccatgca acggaagaaa gtttggacga gacatataaa
     3661 gattacgaaa aagcctatac cgaagtattc aagcgttgtg gcttagaatt tcgctcgatc
     3721 atcggagacg gcggcgcaat gggcgggaaa gactcaaagg aattcatggc gatctcagaa
     3781 atcggggagg atacgatctg ttattcgact gaaagtgatt atgctgcaaa cttggagatg
     3841 gctacaagct actatgtatc aaaaaaatcg catgaaacac agcttgaatt ggagaaagta
     3901 gctacaccta acgtaaaatc aattgacgaa gtagcagatt tcttttctgt tgaacctcaa
     3961 aaaatcatca agtctgtatt gttcattgcg gatgaacaac ctgtccttgt tttggtacgt
     4021 ggagaccatg aagtcaatga cgtaaaactg aaaaactttt tatctgctga ctttttagaa
     4081 gaagcaacag aagaagaagc acaaaaatat ctaggtgctg agtttggttc tgtcggacca
     4141 gtcggtgttt cagatgacgt aaaagtatat gcagaccgtt atgtccaaga tttagcgaat
     4201 gccattactg gagcgaacga aacaggattc cactataaaa atgtgaatcc aaaacgcgat
     4261 ttcactgtgc tttcttatga agatcttcgt tttgtacaag aaggagatcc ttcaccagac
     4321 aacaacggtg ttcttgcctt tactcgagga atcgaaatcg gacatatatt caaaatcggc
     4381 actcgttaca gtgaatctat gggagcaact gtactagacg aaaatggacg agaaaaattt
     4441 gtgattatgg gctgttatgg tatcggtgta agtcgtttat tatcagcaat cgtagaacaa
     4501 cattcagatg aacaaggaat caattggcca aaaggaattg cgccatttga tctgcatatc
     4561 gtccaaatga atctaaaaga cgaataccaa acaagcctga cgaatgaact agaagaagcg
     4621 atgacaaaag ctggctatga agtactggtt gatgacagaa atgaacgcgc aggagtgaaa
     4681 ttcgcggatt ctgatttgat tggctgtcca atcagaatca ctgtcggtaa aaaagcagtg
     4741 gatggaatcg tagaagtgaa aatcaagaaa actggcgaaa tggttgaagt acgtaaagaa
     4801 gaattggcga atactttacc aatcctcttg aaccaaggcg aataataagc gttaaaacac
     4861 agaaaataaa aatagctgaa tcagctgttt ttgacaatct gatgattcgc ttttaaaatt
     4921 ggaactgcga tttgaatcgc agttccaatt ttttaatgta aaactcagaa aaaacgagta
     4981 tactaaaagg tactgaaaaa gaagaggata agatgcgatc aattgcattg tttctcatca
     5041 gaaagggcgt atatctttgt cagaaaaacg agatttattt gaaaaactgt tggaacaaat
     5101 ccaactagag gaagcagaaa aaaatcatcc actgcttact gctggtgaaa tggatagtgt
     5161 agtggtccat cgtaaatcgc gtttatggga atttacgcta catttttcca aaattttgcc
     5221 tattatgcta tatcggtcat tgactcagca tctgactatc gcttttaaag atattgcaca
     5281 agtcaaactg aatatcttag cagatgacca gacattcgat gaacagctct tgcaagatta
     5341 ctgggcccaa gctttggaaa atcagcaatg cgatacacca ctagcacagc aagtattgaa
     5401 aacgcaggta cctgtgataa aagatcgtaa agtcatcttg ccaatcgaca gtacaggagc
     5461 tatttcttat ttgaagcaac aatacttgcc attagtagaa gagttgttcg tcagttacgg
     5521 attcccaaaa tttcgtatcg aaccagaagt ggatgagcag caagcggaac gtgtattgaa
     5581 actttttgaa gaacgcaaac aagaacaggc agaagccttt atgaagcaag cagctgaaag
     5641 tctagttgtc cacgaacaaa agaaaaagga aagaaaagag caatcgccag ctcttgaagg
     5701 acccatccag ctaggacgaa atattccagc cgatgaacca acgacaccaa tgatcagtat
     5761 cgttgaagaa gaacgaagag tcacacttga ggggtacgta ttcgacaaag aagtcagaga
     5821 actacgttca aaacgcaaga tcttgacttt gaaaatcaca gattatactt catcatttat
     5881 cgtcaaaaaa ttctcgaacg gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg
     5941 tagctggtta aaggtacgag gaagtattca agaagatacg tttgtccgtg atttggtcat
     6001 gaatgcgcaa gatatcatag aagtcaaaca tacacctcgc aaagattatg ctcctgaagg
     6061 ggaaaaacga gtagagcttc atgttcatag caatatgagt acgatggatg caacaaacag
     6121 catctctgat cttgtggcac aagcaggaaa atggggacac cgagctatcg ccattacaga
     6181 ccatggtggt gcgcaagcct ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa
     6241 aatcttatac ggcgtggaag cgaatgtcgt agatgatggt gtcccaattg cttataatga
     6301 tgcacatgaa gcattgagtg aagccacata cgtcgtattt gacgtggaaa cgacaggatt
     6361 gtctgcagta tatgacacga tcatcgagct agccgcagtc aaaatgtata aaggaaatgt
     6421 gatcgaaagc tttgatgaat ttatcgatcc aggacatcca ttgtcacgaa caacggtaga
     6481 tctgactggt atcacggatg aaatggttcg agggtcaaag tcggaagaag aagtattgcg
     6541 gatgttcctt gaattttcaa aagatacaat tttggtagcc cacaacgcgg cttttgatat
     6601 ggggttcttg aatactagct acgcaaggta tggtattcct gaagcggcca atccagtcat
     6661 tgatacgttg gaattagcta gatatctgta tcctcaattc aaacgtttcg gattaggtgt
     6721 gttatctaaa aagtttggtg ttagcttgga acagcatcac cgagcaatct atgatgctga
     6781 agcaactggt cacttagcat ggatctttgt gaaagaggca atggacaatc ataacatgct
     6841 ttatcatgat cagctaaatg agcatatcgg agaaggagat tcttacaaaa gggctcgacc
     6901 tttccatgta acgattttag caaaaaatca ggcaggactg aaagatttgt tcaaactgat
     6961 ttcaatgtcg aatgtcgaat acttcgaacg cgtcccacga ataccacgct cccaattgaa
     7021 aaagatgcgt gaaaatctat tgatcggctc agcttgtgat aagggagaaa tattcgaagc
     7081 catgatgcaa aaaggggtag aagaggcaag aaaccgagca aaattctatg attatattga
     7141 agttatgcca aaagcggtct atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca
     7201 tgatttggaa gaaattatcc aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt
     7261 tgttgcaaca ggtaatgtac attatctgaa cgaagaagat gccatttacc gaaaaatttt
     7321 gatcaattct atgggcggag cgaatccgtt gaatcgtcat agtttgccgg atgtccattt
     7381 ccgaacaacc gatgagatgc taactgcatt tcacttttta ggggaagaaa cagcgaaaga
     7441 gatcgtagta gaaaatacga ataaaatcgc agatatctgc gaagaagtga ttccagtcaa
     7501 agatgaactt tatacaccga agattccagg ttcagaagat gaaatatcag aattgagtta
     7561 cacaaaagca aaacaaatgt acggagatcc gttacctgag atcattcaaa aacgattaga
     7621 aaaagagttg aattctatta atggtaatgg cttctcggtt atttatctga tcgcccaaaa
     7681 actagtgcat aaaagtaatg aagatggcta tctagtaggt tcacgtggct ctgttggctc
     7741 tagctttgtt gcgacgatga caggaatcac tgaagttaat ccattagccc ctcattatta
     7801 ttgtccagag tgccaatatt ccgagttttt tgaagatggt acgtatggtt ctggatttga
     7861 catgccagaa aaacaatgtc caaaatgtgg tgcacgctta aacaaagacg gtcatgatat
     7921 tccatttgag acatttttag gattccatgg agacaaagtg cccgatattg atttgaactt
     7981 ctcaggtgat tatcaagcag aagcccataa ctatacgaaa gtattgtttg gcgaagacta
     8041 tgtttaccgt gccggaacga ttggtacagt cgccgacaaa acagcatatg gctatgtaaa
     8101 aggatacgaa agggacaata atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa
     8161 aggcgctact ggagtcaaac ggacaaccgg ccagcatcca ggggggatca tcgttattcc
     8221 cgattatatg gatgtctatg attttacgcc gatccaatat ccagcagacg atcaaaattc
     8281 agaatggaaa acgactcatt ttgatttcca ctcgattcac gataatgtat tgaaattaga
     8341 tattctaggg catgatgacc caactgttat tcggatgctt caagatttat cagggatcga
     8401 cccacaaacg atccctacgg atgacccgga agtgatgcgg atatttgccg gtcctgaagt
     8461 acttggagta agtcaggaac aaatttattc caaaacaggg acattaggta ttccagaatt
     8521 cggcactcgt tttgttcgag ggatgttaga agaaacgcat cctacaactt ttgcagaact
     8581 actgcaaatt tccggactat ctcatggtac agacgtatgg ttaggaaatg cggaagaatt
     8641 gattcgacga ggagatgcga cactggcgga agtaatcggc tgtcgtgatg atattatggt
     8701 ttatttgatc catgctggat tagatagcgg gatggcattt aaaatcatgg aaactgttcg
     8761 taaaggacaa tggaacaaga tccctgatga attgcgagaa acctatctat cggcgatgaa
     8821 agaaaataat gtgcccgact ggtatatcga ttcttgttca aaaatcaaat atatgtttcc
     8881 gaaagcccat gctgcagctt acgtattgat ggctcttcgt gtcgcttatt tcaaggttta
     8941 tttccctatt ttgtattact gtgcatactt ttctgtacgt gcagacgact ttgatctagt
     9001 ctccatgtgc aaaggaaaag atgctgtgaa acaagcaatg aaagaaatca cggacaaggg
     9061 attagatgct tccgtaaaag aaaagaatca actgacagtg ctggagttag ccaatgaaat
     9121 gctggaacga ggattcaaat ttggaatgat tgatttgtat aaatcggatg ccgttaactt
     9181 tgttattgaa ggtgatacgt tgatcgcacc atttagagct gtcccaagtt tgggaaccaa
     9241 cgtggcgaaa caaatcgttg aagcaagaaa agacggtcca tttttatcca aagaagatct
     9301 tgccacccgc ggtaaagttt caaaaacatt gatcgaatat atgaacgaca atggcgtatt
     9361 gaaagatcta ccagatgaaa atcaattatc gttattcgat atgctataag catctgtttt
     9421 cctgtctgct tgtaatttta agcagagtat ggtatagttc ttataggtaa tgatactaac
     9481 tgtgagtgag cggatttttc gctcactctt tttaatggaa ttacgcactt aatttttaag
     9541 gaggcgaaac ttttgagtag cgtcgttgaa acagtcacag aaatggtgac accgatttta
     9601 gatgaacaaa attttgaact tgtcgaagtc gaatttgtaa aagaaggaaa aagctggttc
     9661 ttacgagtat ttattgacaa agaaggcggt atcgatatcg aagaatgcgc ctttgtcagt
     9721 gagaaactga gtgaaaaact tgatgcagca gatccagatc cgattcctca agcttatttc
     9781 ctggaggtat cctccccggg agcagagcgg ccattgaaaa aagaaagcga ctatgaaaag
     9841 gcacttggtg attatgtcca tgtctcctta taccaaccag tagatggcga aaaacaatat
     9901 gaaggatttc ttcaatcatt tgatgctgaa cagcttacat tgaaaatacg cataaaaaca
     9961 cgagaaaaag aattgacttt tgaccgaaaa aatattgcaa aagctcgctt agctatccaa
    10021 ttttaatcac ggagaggaat cagaaaaaaa tgagtaaaga aatgttaaat gcgttagacg
    10081 ctttagaagc tgaaaaaggg atctcaaaag aaatcgtgat cgatgcttta gaagcagcat
    10141 tggtttctgc atacaaacgt cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc
    10201 agaaaaaagg aaagatccat gtctatgcgg taaaagaagt aacggaagaa gtgatggatt
    10261 cacagttgga agtctcattg aaagatgcac ttttaatcaa tccagcatat gagatcggtg
    10321 atacgatccg ttttgaagta acaccaaaag attttggacg gatcgctgcg caaacggcta
    10381 aacaagttat tttacagcgt gtacgtgaag cagaaagaac gattatttat aacgaattca
    10441 gtgcttatga aaaagacatc atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt
    10501 atgtcaatct tggtaaaatt gaggcagttc tttctaaaca agatcaaatg ccaaatgaat
    10561 tttatcaacc acatgatcga atcaaagttt atgtttcccg agtagaaaat acgtctaaag
    10621 gtccgcaagt atttgttagc cgcagccatc cagacctatt gcgtcgctta tttgaacaag
    10681 aagtaccaga agtctatgat ggaatcgttg aaattgtcag catcgcccgt gaagctggcg
    10741 atcgttcaaa agtggcagtt cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg
    10801 tcgggccaaa gggacaacga gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg
    10861 atatcgtcga atggaatgaa gatccagctg tattcatcgc aaatgcattg aatcctgcac
    10921 aagtcatgga tgtgatcttc gatgagacaa atccaaaggc ctgcacagtt gtcgttccag
    10981 actaccaatt atcgctagca atcggtaaac gcggacaaaa tgcacgttta gctgctaaat
    11041 tgactaacca taaaatcgat atcaaatcag aatctgacat gacggaattt tatgaaaatc
    11101 aagaacaaca aaaagaaaca gaagaactgc atgatgaagc aatcgttcag tcagatttaa
    11161 cagacgatga atatgaaaca attgcattca acaatgaaac ggtcgaagaa aaaccagaag
    11221 cttaacttta gtaaggaggc aaagagatga aacaaaggaa aattccttta cgcaaatccg
    11281 tggtttcagg tgaaatgaaa cctaaaaaag agatggtccg aatcacccgt tcaaaagaag
    11341 gggaagtatc gatcgatcca actggaaaaa tgcctggacg aggagcgtat gtctcacttg
    11401 agccggaaga agtacaacaa gcttgggata aaaaaattct cgatcgagta ctcgaaacaa
    11461 cattaacaga tgagttttat caagaacttc ttgactatgt cactcaccaa aaagcacgta
    11521 aagagttatt tggaaaatga atacagttaa tcggcaaaaa gccatgaatc tcatcggtct
    11581 agcgatgcgc gcgggaaaac taatcacggg tgaagaactg accattgctg aaatccgcaa
    11641 acaaaaagca aaattggtgt ttgtggcaac cgatgcaagt gagaatacaa gaaaaaagat
    11701 aaaagataag agttcatatt atgaagttcc ttgctttgag ctattttctg aagcagaaat
    11761 cacgcagatg atcggaaaac ctcgcaaggt attcggaata atggatgccg gatttgcgaa
    11821 gaagaccaag gagctaattg aaggttagga aggtgattgc atgggcaaca aaagaatcta
    11881 tgaattagca aaagaactca aaaaatctag taaagacgtc gtggaaaaag cacaacaact
    11941 tggcatcgat gtgaaaaacc atatgggtgc gattactagc agtgatgaga agaaattgca
    12001 acaagcgttc aaaaacccaa gcacgaaaca acaagctgca caacaagcaa gcagaaaacc
    12061 agccaatcaa cagccgaacg atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa
    12121 taaaaataac cgcagttatc aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa
    12181 aaaaccaaca acaaaccaaa ataatacggg aaataccagc agccaaaatc gtcaaggaac
    12241 gagccaaggc ggcaatcaaa accgtactaa caataacaac aaaccaaaca ataacaatcg
    12301 cggcaaattt aataataata accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca
    12361 gcaacaatca actaaaccag ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt
    12421 agagtatact gaaggtatga acgtagcaga tattgcgaaa aaaattcatc gtgagccagc
    12481 agaaatcatt aaaaaattat ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga
    12541 taaagatacg attgaactgt tagcgacgga ctatggcatg gaaccacaag aaaaagtcca
    12601 agttgacatt gctgatatcg ataaattctt cgaggcagaa gaagtcgatc cagataaatt
    12661 agtctcacgt ccgccagtag taacgattat gggacacgta gaccacggga aaacaacctt
    12721 attggataca ttgcgccatt cacgtgtaac aagcggcgaa gccggcggaa tcacacagca
    12781 tatcggtgcc taccagatcg atattgatgg taaaccaatc actttcttgg atacaccagg
    12841 acatgcggcc tttacaagta tgcgtgcacg tggagcaagt atcacagata tcacgatctt
    12901 agttgttgct gcagatgatg gcgtcatgcc acaaacagtt gaagcaatca accatgccaa
    12961 agctgcaggc gttccaatca tcgtcgctgt aaataaaatt gataaaccag gtgcaaatcc
    13021 tcagcatgtt atgcaagaat taagtgagta tgaattaatt cctgaagcat ggggtggaga
    13081 aacgattttt gttgagatct cagcgaaatt cggccaaaac atcgaagaat tgctggaaat
    13141 gatcctgtta gtcgcagaag tagaagattt aaaagcagat ccaacacaac gcgcaatcgg
    13201 aacagtgatc gaagcacgtc ttgataaagg taaaggtcct gtgtcaacct tacttgtcca
    13261 agaaggaaca cttcacgttg gtgacccaat cgttgtcggt aatacttacg gacgtgtccg
    13321 tgtaatgaca aacgatcttg gccgtcgcga taaagaagca ggacctgcaa cgccagtaga
    13381 aatcactggc ttgaatgatg tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga
    13441 aaagacagca cgtgcagcgg gtgaagaacg cgcaaaacgt gcattattgg aaaaccgtgc
    13501 tgccacaagc cgtgtgactt tggacaactt atttgaaagc ctgaaagaag gcgaattgaa
    13561 agaagttaac gtaatcatca aagcagatgt acaaggttca gcagaagcac tggctgcatc
    13621 attgaagaag attgatgtgg aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc
    13681 aatcaatgaa agtgatgtaa ccttagcagc agccagcaac gctattatca tcggtttcaa
    13741 cgttcgtcct acaccgcagg caaaagtgca agctgatact gaagaagtcg atattcgtct
    13801 tcaccgtatc atctataaag ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga
    13861 ccctgaattt gaagaaaaaa tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc
    13921 taaagttggt acgattgcag gtgcctatgt aacagaagga tatattcgta gagatagcgg
    13981 tgtccgtgta atccgtgacg gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg
    14041 attcaaagat gacgtgaaag aagtcaaaat gggttatgaa tgtggtgcga tgattgaaaa
    14101 attcaatgac atcaaagtag atgacgtaat tgaaggattt atcatggaag aagtcaaaaa
    14161 tgactaaaaa ctgataaaag gaggccaatc attatggcta actaccgtga tcgccgagtc
    14221 ggtcaagaaa tcttaaaaga agtcaatgat attttgcgca aaaaggtccg tgaccctcgt
    14281 gtggagaacg taacgatcac agatgtgaaa gttacggggg acttacagca agcaacgatc
    14341 tattacagca ttttatctga cttagcatca gataagaaaa aagcacaaga tggattgaat
    14401 aaagcatcag gattgattcg tcgagagtta gggcacaatc taagtattta taagacacct
    14461 gaattgttct ttgaacttga tgaatcagtt gtctatggga accatatcga tgaattgctt
    14521 cgcgacttga acaaagacta atcaaaaaat cccgcaaatc ctgtgaaatc aggggttgcg
    14581 ggattttttt gttttcatta agacgtatta cctaagagtt ccatgacttt agtctggtag
    14641 cgtgtcagct aaccaaaaaa gagaaaaaga aaagtaaaaa attcgacttt cttttttcta
    14701 tagtatataa atttttgcat atagataaag atcaagatac cgataacgga agctagattg
    14761 taatctattt tagtgacagt caggtatagc caagtaacta gaagatcagt ggaacctgca
    14821 ccataaaagt ttgagtttgt cggtccgttg acagttaaaa gaaaaatcac gttgaagtta
    14881 ttgatatttc cgattacgcc gcttcgattt ggtcacgatc caaattttgg ataatccctg
    14941 tagaaattag catagtagct gggataccaa ttcacatatt gacaaagata acagagactt
    15001 ttacataaaa cggatcaatc aggaaaggaa ttgctcgatc cagccccagt tttccaacaa
    15061 tgtgttaact ggcctagcgt cattcagcaa attatgcatg atgaggagcg aaatgaactg
    15121 cggaacagcc attgtgataa cgacaagttt tttggatcaa tgtaaaaaac gtcagcgaaa
    15181 ggaaaaattt cgtggttttt tatacctgtt catttggcta gatataaaat ccaaacaaat
    15241 gaacagaagt gcttattagg tttcaattgg gagtatctgc gctagaataa gagtaataat
    15301 gaaaaggagt gttatgtatg gattattcta gtaaagaata ctttgataaa atgacggctt
    15361 ggtggcgtgc tgctaattat ctttctgttg ggcaacttta tcttaaagat aaccccttac
    15421 tgagacgtac actgaaacca gaagatgtca aaaaacatcc aatcggtcat tggggaacga
    15481 ttcctggcca aaattttatt tatgtccatt tgaaccgagt catcaataaa tatgatttga
    15541 acatgtttta tatcgaaggg ccaggacacg gcggacaagt catggtatcg aatgcttatt
    15601 tagatggaag ttacacagag atctatccag aagtaacgga agatgagaca ggaatgcaaa
    15661 aattattcaa gcgattttca tttcctggag gcattgcttc tcatgcagca ccagaaacac
    15721 caggttctat ccatgaaggc ggagagcttg gatactctct ttcacatgca gtcggagctg
    15781 tattagacaa tcctgaagta atctctgctg tcgtgattgg tgatggtgaa gcagaaaccg
    15841 gtcctttagc tggaagctgg ttctcaaatg tctttatcaa tccagtgata gatggagctg
    15901 ttttaccgat ccttcattta aatggtgcaa aaatagccaa cccaacgatc ttggcacgta
    15961 aatcagatgg agaattagca aattacttta acggacttgg ctgggagcct ttctttattg
    16021 aaggaaatga tccagaaaaa ttaaatcctg ttatggctga aaaaatggat caagcaatcg
    16081 agaaaatcaa atccatccaa aaagaagctc gcctgaagac agcaacagat gtcgtgatgc
    16141 caaaatggcc agtattgatc gtacgcacac caaaaggatg gaccggtcca aaagaatggg
    16201 acggggaacc aattgaagga accttccgag cacaccaagt accgattcct gtagatcaag
    16261 aacatatgga tcatgcagat gctttactcc gttggctgaa atcctatgaa ccagaaaaat
    16321 tatttgatgc acaaggacgt attttagaag aaatcagaga aattgcgcct actggtgacc
    16381 aaagaatggc aaaaaatcct atcaccaatg gtggaatcga tccaagaccg cttattatgc
    16441 cagattggaa aaaatatact ttgcaatttg aaaaaccagg ctccgttaaa gcagaagata
    16501 tgaccgaact tggtaaattc gttcgcgaga tcattgagaa aaatccagaa aatttccgta
    16561 tatttggacc agatgaaaca aaatcaaacc gattgaatca agttttcaaa acgacaaatc
    16621 gccaatggat ggaaaaaatc gaaccagaaa atgatgaatg gctttcgcca tctggtcgcg
    16681 tcatcgacag tcaattatcg gaacaccaag atgaagggtt cttagaaggg tacgttctga
    16741 ctggacggca tggttttttt gcaagttacg aatcattctt gcgagtggtt gactctatgt
    16801 tgactcagca cttcaaatgg atacggaaat cacatgacct ttcttggcga aaagattatc
    16861 cttcgctgaa tctcattgca agctcaacag tcttccagca agatcacaat ggatatagcc
    16921 accaagatcc aggaatattg acacatttgg cagaaaagaa ggcggagttc atccgtgaat
    16981 atctgcctgc ggatgcaaat acattgctcg cagtcatgga taaagcattc cgctcgtcag
    17041 aaaaaatcaa tttgatcatc tcttccaaac atccgcgtgc tcaattttac tcagcagaag
    17101 aagcagccgt cttagtgaat gaaggcttga aaatcattga ttgggcttcg acagcaaaag
    17161 aggaggaacc tgaattggtc attgctgctg caggaacgga atctaactta gaagcattag
    17221 cagctgtaac tttgctgctg gaagaattcc caaaactaaa aattcggttc atcaatgtcg
    17281 tagatttatt gaaattacgt catccaagtc aagatccgcg tgggttgagc gatgaagaat
    17341 ttgatcaata ttttacaaaa gacaaaccga tcttatttgc tttccacggt tatgaaacat
    17401 tggtccgcac gattttcttt gatcgtcata accatcattt aatgattcat ggctacaaag
    17461 aaaatggaga tatcacgaca ccatttgaca tgcgtgtagt aaatgaactt gatcgttatc
    17521 atttggcaaa agacgcagct ttaaaaatca aaggaagtca agcagaagat tttgcggaaa
    17581 aaatggatca gaaattacaa gaacatcaaa actatattcg tgaaaatggt attgatctgc
    17641 cagaagtatt ggattggaaa tggaaaaatc ttgaccaata aaatgataaa atcgatcccg
    17701 caaaacagaa ttttttacat tgtattattt g
//
LOCUS       DAZUMM010000056        17591 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000056 DAZUMM010000000
VERSION     DAZUMM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.056"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..2149)
                     /gene="tkt"
                     /locus_tag="ACG8E6_001860"
     CDS             complement(152..2149)
                     /gene="tkt"
                     /locus_tag="ACG8E6_001860"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFS5062268.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2251..2502)
                     /locus_tag="ACG8E6_001861"
     CDS             complement(2251..2502)
                     /locus_tag="ACG8E6_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFS5062269.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2649..3272
                     /gene="lexA"
                     /locus_tag="ACG8E6_001862"
     CDS             2649..3272
                     /gene="lexA"
                     /locus_tag="ACG8E6_001862"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFS5062270.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3452..3670
                     /locus_tag="ACG8E6_001863"
     CDS             3452..3670
                     /locus_tag="ACG8E6_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062271.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3959..4471)
                     /locus_tag="ACG8E6_001864"
     CDS             complement(3959..4471)
                     /locus_tag="ACG8E6_001864"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFS5062272.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4485..6782)
                     /gene="recJ"
                     /locus_tag="ACG8E6_001865"
     CDS             complement(4485..6782)
                     /gene="recJ"
                     /locus_tag="ACG8E6_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFS5062273.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6924..7367)
                     /locus_tag="ACG8E6_001866"
     CDS             complement(6924..7367)
                     /locus_tag="ACG8E6_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFS5062274.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7368..8156)
                     /locus_tag="ACG8E6_001867"
     CDS             complement(7368..8156)
                     /locus_tag="ACG8E6_001867"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5062275.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8153..9094)
                     /gene="rnz"
                     /locus_tag="ACG8E6_001868"
     CDS             complement(8153..9094)
                     /gene="rnz"
                     /locus_tag="ACG8E6_001868"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFS5062276.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9271..9858)
                     /locus_tag="ACG8E6_001869"
     CDS             complement(9271..9858)
                     /locus_tag="ACG8E6_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFS5062277.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10079..11392)
                     /gene="obgE"
                     /locus_tag="ACG8E6_001870"
     CDS             complement(10079..11392)
                     /gene="obgE"
                     /locus_tag="ACG8E6_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFS5062278.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="ACG8E6_001871"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="ACG8E6_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS5062279.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="ACG8E6_001872"
     CDS             complement(12784..13719)
                     /locus_tag="ACG8E6_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5062280.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13905..14864
                     /locus_tag="ACG8E6_001873"
     CDS             13905..14864
                     /locus_tag="ACG8E6_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFS5062281.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWMPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDVDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEYDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(14950..16044)
                     /locus_tag="ACG8E6_001874"
     CDS             complement(14950..16044)
                     /locus_tag="ACG8E6_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFS5062282.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            16233..16994
                     /locus_tag="ACG8E6_001875"
     CDS             16233..16994
                     /locus_tag="ACG8E6_001875"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5062283.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(17109..17549)
                     /gene="fabZ"
                     /locus_tag="ACG8E6_001876"
     CDS             complement(17109..17549)
                     /gene="fabZ"
                     /locus_tag="ACG8E6_001876"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS5062284.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT         5050 a         3829 c         3084 g         5628 t
ORIGIN      
        1 acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc cgaaaacgaa
       61 aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac ttgcagacag
      121 ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac gtattcacga
      181 cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca gatgcaccga
      241 aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat ccaaatgatg
      301 cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt tctttatatt
      361 cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa acatctttgc
      421 ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa cctgttgcaa
      481 tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg ccttttttca
      541 ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga cgactcaata
      601 caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca cgtgtttcat
      661 ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat gctagttgtt
      721 caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt gttaagacgt
      781 aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga taatcgacaa
      841 agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata ccattcatag
      901 ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca taatgttcag
      961 gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag tccgctgaac
     1021 caccccagaa gcttggtata gctttcgata attcttggat gacttcttta cttgaaacac
     1081 ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag ttttctggta
     1141 gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct tttttataat
     1201 tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc atcgtttgat
     1261 ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa ccataaactt
     1321 ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca gatgttcctt
     1381 ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt ttgtctgttt
     1441 cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg tctttcacta
     1501 agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg aaagcttttg
     1561 aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat ttccctagtt
     1621 tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag tctccgtcac
     1681 cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta tacgttgctg
     1741 caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt cctaatggcc
     1801 ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt gttttagaat
     1861 cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct aaatgaagca
     1921 agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg cggtcagccc
     1981 agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca tatgccatag
     2041 gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact gcttcaatac
     2101 ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa ataacccctc
     2161 ctattaattt aggttttttt tacctttatt tcttacactt tcattctata agatgtcaca
     2221 cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt tgtgtaagcc
     2281 ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct caggatcgac
     2341 gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca aatactcttt
     2401 gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct tctttttacg
     2461 tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc ctccttttac
     2521 tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa ataaaatacg
     2581 aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag tgaaagaagg
     2641 tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga tatatccatg
     2701 aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa gctgttcagc
     2761 tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa gggtttatcc
     2821 aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta gaaaagattg
     2881 ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa cccattttag
     2941 cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc gaagaaggca
     3001 gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata ttggatggtg
     3061 acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc attgcaatga
     3121 ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt ttccgtctgc
     3181 agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata ttaggacgag
     3241 tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga aaacttttta
     3301 aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc ttctttcaaa
     3361 tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat atacgaacaa
     3421 ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc ttggcttagt
     3481 attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc cagctttact
     3541 tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga agtaccgtca
     3601 atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact acagatccag
     3661 gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa acttttacgg
     3721 gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct aaagccagtt
     3781 tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttctact ggtctttgct
     3841 tgtttccatt tataaaaaaa tagcgaccct caaaagtcag atagagaatc tgacttttga
     3901 gggtcgcttt aaaatcgctt ctttaagaat aacatcaaga atagcttctt ttttctgctt
     3961 aatattccat taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca tggagatcca
     4021 tcaattcgat caaaaatgcg cagcctacga ctacaccgcc taattgttcg atcagttcga
     4081 tcgtcgcttt gattgttcct ccagtagcta gcaaatcgtc acaaatcagc acacgttgac
     4141 ctggtttaat ggcatcctta tgcaatgtca atgtatctgt tccatattct aatccataag
     4201 tcacttcgat ggtttctcga ggtaacttcc cttttttgcg gactggggca aaaccgactc
     4261 ctaactcata agcaacagga catcctacga tgaatccacg ggcttctggc ccaactacca
     4321 tatcaattcg tttttctttt gcataattta cgatttgttt agtagcttcc cgataagcgt
     4381 caccatctgc catcagtggg gaaatatctc gaaacacaat tccttttgac ggatagtcgg
     4441 gaatactggc aatataatct tttaaatcca ctctttgttt cctcctaggt tgttaaccat
     4501 ttctttatgg taggaatatc gctcaaaagc aaaaattcct caactttgat tttattgagc
     4561 cgtcgctggt ataatctgct ctcagtcaaa ggatgacttt ctgggctagc gactttttgt
     4621 aaaacaccgt tctctattgt aacaaatttc aattcaaaaa acacctgtat cataaaaatt
     4681 aataattttt cagggatacg caaatattga gctattgctt ttattttatg acgtatatca
     4741 attcgttctt gagaatggat caatttaaac aattttgcgt actgttctct tgtgcctacc
     4801 ccattcaaat aggcctcatc aggtgaaatc cccatcagat aaacacgatt gaatgaataa
     4861 gaccgaaaaa cttctttcag atccgctaaa tcatctggac aatccataac aactagagag
     4921 tttggttttc ttttttcaat tattgccttt acagaatcta gtccttcgaa aacaatcgca
     4981 gcttcctgga taatctcagg cacgaatttc aacgtcgaag aatcaaatgc taaataaagg
     5041 ctaccttccg acgttcggct ctgttcacgg aaacgttttg cccgccaatc aaatacttgc
     5101 agaccaccca cagaaaaatc agataccatc agctgcggtt tcttacgccc gttccattca
     5161 ttgatcgaaa gctttcctac cacatctacc tgattattca gcagttcttc ttcttgagta
     5221 ccaaaaccaa aagcgaccgc atcaagctga ctcgatgcat cggaaaggac aaatttcaga
     5281 tgctgttggt ttgctccgat tcttttgata ttttctgctt ggacttgtcg aaataaaaac
     5341 tgaggcaaag gattatccgt accaaatggc gccaatagtt tcagctcatc aattcgctct
     5401 actgttactt catttggcag caatacttca tcaattctca actcaggccc tctcatcaag
     5461 tcaatttgat gatcgactat atattggttc atcttctcct gtagtatagt gacattttca
     5521 cttggcatcg ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa taaatctcgc
     5581 atctggtcaa gcatttcaaa aagattcaag gcttcaatac tacgtccaga tcctttagct
     5641 gatccatctt cttttaaagt aaggacaagc gttggtttac ccgtttcatt cattattttc
     5701 ccagcaacaa tgcccaaaac gccttcatgc catcctcggt ttaccaatag atggatatga
     5761 ttctccttat tgaccatcgc taatgcttct tgtgttatct gctctacgat tgcttttctt
     5821 tcttcattga tctcattcaa tttttttgct tgaacggatg cttcctcttc atcaaacgtc
     5881 gcaagtaacg agacggcagg acgtggatcg cccatccgac cgattgcatt tagtctggga
     5941 gcaatcgaaa aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc gctttcttca
     6001 aatagcgcct gaagtcctat ccgttctgaa tgatgaatgg catcgattcc taatgaaaca
     6061 aggattcgat tctcatcagt caatgaaact aaatcggcga tcgttcctat cgctactaaa
     6121 tccaaaaatt ctgcaggcgg ttcttccaat aatgcagaag ctactttgaa tgctactccg
     6181 actcctgcca aatctggaaa tggatagtgc cccgctggat gacgagggtg aacaatcgca
     6241 taagcgtctg gtaaattctc aggtagttcg tgatgatctg taatgatcac gtctacacca
     6301 gcttgctgag cgtatgctac cgcatcattt cctgctaccc cgttatcaac agtgatgatc
     6361 aactggattc cttcttcgat cttttcttgg tataccgatt ggtttggtcc atacccatgc
     6421 tcaaatcgat ttggcaggaa tacttctact tctgcaccaa gtaattccag tgtttctttc
     6481 attaccgttg cactggtgat cccatctgcg tcataatcac cgtaaacgag gattttttct
     6541 ccactgatta cagcttcttg gattcggctg attgctttat ccatatcgtg tatcagatac
     6601 ggatcatgta attcttgttc ctttggatgt aaaaatcggt tgatatcctt ttcctcatgg
     6661 taacctcttt gccacagtaa cttaccgaca aatggagata atttcagttc acggatcatt
     6721 tggagaaagg attcagaagg ctctgtctcg cttaacttcc aatcaaaatt tgcttgtctc
     6781 actttctcac tcctaactta gttattatag caaaggaaat cagcaaaaaa aagcagaaag
     6841 aaagaataaa aacagatcag atccgctaga cagatgttca gcaggacctg atcttttgta
     6901 cgaccattta tgaaatatcc ctattagtca aaataattga agctttgttt gggcgattga
     6961 gttttttgtt cttccggcgc tttcaattga ctgatttgtg cggttttcgc ttgtaactcc
     7021 gcctgataag cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg ctgttcttct
     7081 tttgcttctt caattttctt ttgaagttct gtttgatatt ctgacatttc ctgagtcagc
     7141 tgtttgattt gctttttttg ctgccatagt gttgccgaag cagtcaaaaa tacaatcagt
     7201 gcaccaatca gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa ggaagtgaag
     7261 ccaaaactta caggaacatt tttattgttc aatacagcaa aaagaacaat aatcaaaact
     7321 aaaacaaatc caagaatcac attgctttgc tttttcaatt ccttcaccta ctttttgtta
     7381 aaaattccgc tggctaaata atcaccaatc gttggaaaca gcgtataaaa ttttgatgca
     7441 acttctaaaa taaacggttg attgatttcc cgttttggat gtctcatatt gcggacgatc
     7501 atttttgcca acttggcagg ttctaaaacg atttgtccga ctttttccaa atagcttccg
     7561 cttggatcag ccttatcgaa gaaatttgtc tcgattggac ctggattcac tgtcgtaaca
     7621 gctacgccaa gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag gactgcaaat
     7681 ttcgtggcag aataaaccgt tgattttgca gttgccattt ttccagccat agaggcgata
     7741 ttaatgatat gaccttgtct tctttctgcc atatcgatcg caaatttttg tgtcaaaacc
     7801 atcattccta agatgttgac ttcaaacata tcgtatgctt ttccaaggtc gaatgtaagg
     7861 aagtcttcga aaatcccaaa acccgcattg tttaccagaa tatcgacgcg cccgacttct
     7921 tccatcacct tttcatataa ttcgtccacg ctttcaggat tgctgacgtc taccttgaaa
     7981 gcataagcct ctttgccgct caagcgtctg cactcgtctc tgacaccttc tacaaatgcc
     8041 attcttcgtg cgcaacttat aacgattgct ccttgctttg ctgcttcata acagatttgt
     8101 gcgcccagac cgcctgaact acctgttact aaaacaactt tatcttctaa attcatgcct
     8161 cttcctcctc gcgaaatgga atctcaatgc tatcaaaatc tttcataatc gtcgtgtttt
     8221 caaacacttc ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa tatctcgcac
     8281 tgatatgagt aagtagcagc cgttttgcct gcgcttcttt tgcaacttca gcagcttgat
     8341 gagtcgtgga atggaaatag ttgtgcgcca ttcttgcttc atctttatta aatgtgcttt
     8401 catgtaccag aatatcgcta ttttccgcaa gcacaacgga attatgtgtt tttctcgtat
     8461 cgcctaaaat agtcacgatt cgtccttttt tatctggacc tacgaaatct tttccattga
     8521 tcgtccgccc atcttctagt tggatcgttt caccttgctt cagtttgccg tacagtggtc
     8581 cagctggaat acctaattct tttagacgat ccacctgcaa ttctccttta tgatcatgtt
     8641 caaccacacg ataaccaaaa ctgtcgatcc catgatttag tttttttgta taaacagaaa
     8701 attgctgatc agtaaaaatc ggggctgttt catttagctc aatgaatttc agcggataac
     8761 ttaaacgcgt ttgcgaaata cctaaagaga cttttatgta ttcctctatc ccttttggcc
     8821 cgtaaatctc taatggcgta tccccacctt gaaatgaccg gctgctgatg agcccgggaa
     8881 gtccaaaaat atgatcgccg tgtaaatgtg taatgaaaat tttaccgatt tttctcggac
     8941 gaatagtcgt tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac aaccaaactt
     9001 cattgcgctc gtctaaaagt ttcaatgcta tacttgtcac atttctatgt tttgcaggaa
     9061 cacctgcacc tgttcctaaa aattgtaatt ccataatcta cctgactttc tttaagttcc
     9121 tctttaattt tagggtaaaa agaagggata atcaaccgta agtcgagaat gggttcaaat
     9181 taaaaataac gttctaaagc gatagcgttt tctttgaaaa ttactataaa aacagcagcg
     9241 aacccgtttg agatcctctg ctgtttttta ttaatttgaa gatccagaac tttcattgtc
     9301 gttggaagtt tgcggatgtt tgtccttcca agtcttttgt gcagcttctt tcgcatgatt
     9361 ttcagcttct ttcgcatcat gcacatcttt ttcgacatct tcataatcca agcgagtaat
     9421 ttctccgcct aattccatca ttaccaattg attcagtggg cgattgtcct cttcatcagc
     9481 aatcaagatc agtcctgtag ctccttcacc gattttattt cctacaaagt cgaacacggt
     9541 ctgggcatct tttatttctt tggcatcttg tgtcgcaccg atcattcctc cggcaaacca
     9601 tcctaataaa atccctagcg gaccacctaa tacaccaacc aacattccga ttaatccacc
     9661 cgtagaagat ttgttattac cggtaaagtc gataaaatct tctattttga attgatgtgt
     9721 cccattagga tcatggctga caactgccat ctgttctcct ttaatcgaac gaacagcatg
     9781 taactttttg atttctgaaa atgcctgata tgcctggctt tctacttgaa aatttaaaat
     9841 gattactcgt ttgctcatta aaaacgcccc tttcatacct tcttgttatt tattttacct
     9901 ttaattgtaa aataagaaaa gaaaagcact ctttttttcc aataaaatag ggctgtgaca
     9961 aaagtcgtat cacagctcct ttttccgaat aaacggcgtt cttgaagctg atcacttctg
    10021 tcacaacctc ccttaaatct gaatctatat tttttggtat ttgtttttat ttgcttgcct
    10081 attcgacaaa ttcaaattca aactcaccga tacgtacaag gtcgccatct ttcgcaccgc
    10141 gtgcccgaag agcttcatcc acacccatac cacgtaattg acgagcaaaa cgcatcactg
    10201 tttcatcatg atcgaaattc gtcatttgga ataatttttc gatcttctca ccagataaga
    10261 tccacgttgc atctgaatca cgatcgattt ggaactcagg accttctgga ttgaatccat
    10321 agtgtacagt ttcttcttcc agttcttctt cgtacaatgg gaattctggc gttacttcta
    10381 acaaatctgc tgtagcattc aataacgcgt ccaatccttg tcgagtcact ccagaaattg
    10441 gaaatactgg gatgtcatca gcaaattcat cttctttttc cttgttcagc tgttctttga
    10501 atttcactag attttccggt gcatccggca tatccatttt gtttgccacg atgatttgcg
    10561 gacgctctag caagcgtaag ttataagtag ataattcttt gttgatggca agatagtctt
    10621 cataaggatc tcgtccttcc atcccactca tatcgatcac atgtaaaatt actcgtgttc
    10681 tttcgatatg acgaaggaat tgtgtaccca gcccaacacc ttgcgaagct ccttcaatca
    10741 agcctggcaa gtctgctgcg gcaaaacttc tgccatcact tgtagtaacc attcctagat
    10801 taggtacgag cgtcgtgaaa tgataagcgc cgatttttgg acgagcagat gaaataacgg
    10861 atagtaaagt cgatttcccg acagatggaa aacctacaag tccaacgtct gccaatactt
    10921 tcaattctag ttcgattttt cgctcttgtc ctggttcacc gttttcagca atttccggtg
    10981 caggattccg aggagaggca aaacggatat tccctcgtcc accgcgtcca ccttttgcca
    11041 caactagtgt ttgaccattt tcaattaaat ctcctaaaag agcaccagtc tccgcatctc
    11101 ggacagtggt tccttgtggt actttaacat aggtatcttc tgaaccacga ccgtgcatcc
    11161 ctttactcat cccattttca cctggctgcg ctttgaaatg gcgattgaac cgaaaatcca
    11221 tcaatgtacg taacccttca tcgacgatca agatgacatc gccgccacga ccaccgtctc
    11281 cgccagccgg tccgccgtca ggaacgtatt tttcgcgacg aaatgcaacc attccgtctc
    11341 cgcccttgcc agctttcaca tcgatcgtta cttgatctaa aaacatggac ataattaagt
    11401 cctcctatta ttcatttatt tatttgactg cctgaatttc cacaataaac aaaagtctta
    11461 cttattttat cggtgaaatc tgattttgtc tatcatttca gcaaaaagat tgtttacctt
    11521 cttctttttt tatcatttgg taaggtttcc aacctttttt ctgctaccaa gtataatttt
    11581 ttgacaaaat attctttgcg tagcagaata acagtgtatt aattgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataacccg cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc
    13861 aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc
    13921 ttaccataga cgaactgaca atgatagaat catattatct tcaacataat aagccggttg
    13981 aaatcgctaa ccgaatggga cgtgctatac aaactattta taatgtagtc aataagttca
    14041 agcaaggcaa gactgctctt gattattggc accagtataa agaaaataag aaaaaatgtg
    14101 gtagaaaagt cattcaatta cctgctcatg aagtagatta cattaaagag aaagtcactc
    14161 ttggttggat gcctgacgtc attatcgggc gaaaagaaag gcctgtttca tgcggtatga
    14221 gaacacttta tcgtttattt tctaaaggaa tatttgatgt tgacacacta ccgatgaaag
    14281 gtaaaagaaa acccaatggc catcaggaaa aacggggaaa acaacagtat cagcgctcaa
    14341 tccatgatcg acctgataat tatcctgatt tcaattctga gtttggtcac cttgaatatg
    14401 atacgatcgt tggcattcat cataaaagtg ccgtcattac tttagttgaa agattatcta
    14461 aagtcattat cacgattaaa cccaacggcc gtaaggcatt agatattgaa actgccctta
    14521 atcaatggtt ttctcgcttc cctaaaaact tctttaaatc tattacgttt gactgtggaa
    14581 aagaattttc taactggaaa gccattagta accaacatga tattgatata tattttgcgg
    14641 accctggaac accttctcaa cgcccattaa acgagaattc taacgggatt ctgcgtcgta
    14701 atggactgcc gaaatcaatg gattttagaa aagtgaatca gacatttatt tccagtgtca
    14761 gcaatcaacg taatcatatt ccaagaaaat cattgaatta cagaacacca atcgaaatat
    14821 ttttgagcta tgtacaagaa gcattttatt ctagcttaat ttgacaaatc ataacctata
    14881 aaaaaacaaa ataaaaaagc agaaataact ggctatttct agcggattat ttctgcaata
    14941 actaattatc tattttttat tttcaattaa tttaaagctt gaccatggtt tcaaaaagtc
    15001 aaggaggaat aattcgtcat cggagattct tcctacaaca ttgacacgtc cgtcgttttt
    15061 catatccttc aaggcgattt gtgtttctcc tttgtattga ccgtaccctt cattgtcaat
    15121 cagtacatcc ccatgatgca tatctcgcgt attatgaggc gggaaatctt tatctttgta
    15181 gtatacacga gtcatcgttg aacgaaggat atattctgag cgatcccctc gataactatg
    15241 aaggttatca aacaagcaga ttcgttcgtt ttctgtaata ccttcttccg tatctacttt
    15301 caatgttgga taatccgcgt taaatgcttc tgccatttct ttcaattctt cttcacttgc
    15361 gtacgcattt ccaatagaaa tatcatcaat caatcctgta aggattaaat gcttcacttg
    15421 tgtcgcaatt tcaagatctc ggtggtcttc cagcgtgcac aagccatctt gcgtaggcca
    15481 agggccaaac tcagcagatt gcgaattgac gaatgccatc gtattcaaat tatattttct
    15541 gaatttttct gaacaaaaaa caaagtgttc gtatcctaaa ccagagtatc gatgaggata
    15601 aaaattatga gaacctaata aattatccgt gtttggcgaa taactcatga tgttgtcaac
    15661 gtaattcgtg cccgaactca tattgatctc gattttgata ccaaaaggat tgcgcgtcat
    15721 ttttgcttct tctgctcctg tgaatccgat atctaagcgg accccgtagg cacccatctt
    15781 atggaaaaaa gataagtcat cgtaagaaat ctccaactgt tcaaataacg ctggattgat
    15841 atcaaccatt acttccatgc ctaggctatt cgcgtaatca actacttctt taaattctga
    15901 aagaactttt tctttatcat cattgatctg cagcaaactg gtaaaaaccc gtttataccc
    15961 atacttatga gctaaatcca agtacgcttt gtccttttca aatgttgacc gttctggata
    16021 aatcgagatt cctaattttc ccatattacc atttcctcct gcatcttttc gtttttgtat
    16081 ccacttacat tatagcaaac acaaagtaaa tggtctacac cttttctttt tcttttaaaa
    16141 taaatgataa attccaataa tttaagtagg tattttccct ctattttgtt atcattaaag
    16201 ataacaaaat agtaaattgg aaaggaacag ttatgaagaa aaataaagtt attttaagta
    16261 ttagtttagc agcagcattg ctgtttgctg ctggttgttc tgcaaacaac acgacttctt
    16321 ctagttcgtc tacagaaagt tcaaaaagta catctactgc ggaaagcacg actgccagta
    16381 gcgtcgattt gaacgcattg gaattaccac aattgacgaa agatgtcgcc gaaaatgaag
    16441 atctggtcga aatggtgacg aataaaggca caatcaaaat caaattattt cccgaacacg
    16501 caccaaaagc ggttgaaaac tttatgacac atgcaaaaga cggttactac aacggactga
    16561 ctttccatcg cgtcatcaaa gatttcatga tccaaggtgg cgatcctaaa ggcgatggca
    16621 ctggagggga aagcatttgg ggagaaggat ttgaaacaga gatcaacaac cacctttata
    16681 atataagagg tgcgttatct atggctcgct cacaagatcc taacagtaac ggcagccagt
    16741 tctttatcgt tcaaaataca gatgatatgc atgatggtct tttaaaagac gactatcctc
    16801 aagcaatcat cgacgcttat aaaaacggcg gttatccttc actagatggc cattatactg
    16861 tcttcggcca agtgattgaa ggaatggatg tcgtggatgc tattgcaaat atcgaaacag
    16921 atagttcaga caaaccaaaa gaagaagtaa aaatcgataa aatcaatata ctgcaagaag
    16981 caaaacaaaa ataaaaaaat aacgtctgaa tcctgcatag atgacaacgt atgctaggat
    17041 tcaggcgtct tctttttaca taaaaagcaa gtaagcttcc ttttgttttg ctatatcagg
    17101 ttttatagtc aagcattttg gttgacgatg aacgtgaact cgcactcaca gactttttta
    17161 tcatctacat atgctgcaga taacgctgta ccgactgatc ccttcatttt ggtgatttca
    17221 aaatgacatt tcagcacatc tcctggaaca actttttgac ggaatttggc cttattgatc
    17281 ccaccgatat aagctctttt tcctagaaac tcttctgatt ttaggataag tatcgaacct
    17341 gcttgtgcca gtgtttctag aatcaacgca cccggcatca ctggattgtc aggaaagtga
    17401 ccttggaaaa aatcttcatt gattgttaca ttttttgtcg caacgatctt ttttcctggt
    17461 tctaaagtat ctacataatc aatataacaa ataggataac gattaggaat caattccatt
    17521 acttcagtag cagataatac gtaagacata attaaactcc ttctttcagg caattgccat
    17581 tttcattact t
//
LOCUS       DAZUMM010000057        17093 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000057 DAZUMM010000000
VERSION     DAZUMM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.057"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..576)
                     /locus_tag="ACG8E6_001877"
     CDS             complement(31..576)
                     /locus_tag="ACG8E6_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS5062285.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(588..1280)
                     /locus_tag="ACG8E6_001878"
     CDS             complement(588..1280)
                     /locus_tag="ACG8E6_001878"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFS5062286.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1299..1583)
                     /gene="macP"
                     /locus_tag="ACG8E6_001879"
     CDS             complement(1299..1583)
                     /gene="macP"
                     /locus_tag="ACG8E6_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFS5062287.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1596..2159)
                     /locus_tag="ACG8E6_001880"
     CDS             complement(1596..2159)
                     /locus_tag="ACG8E6_001880"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS5062288.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2387..3049
                     /locus_tag="ACG8E6_001881"
     CDS             2387..3049
                     /locus_tag="ACG8E6_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFS5062289.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3173..4369
                     /locus_tag="ACG8E6_001882"
     CDS             3173..4369
                     /locus_tag="ACG8E6_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFS5062290.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4470..5846)
                     /locus_tag="ACG8E6_001883"
     CDS             complement(4470..5846)
                     /locus_tag="ACG8E6_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5062291.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5883..6749)
                     /locus_tag="ACG8E6_001884"
     CDS             complement(5883..6749)
                     /locus_tag="ACG8E6_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS5062292.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6838..7863)
                     /locus_tag="ACG8E6_001885"
     CDS             complement(6838..7863)
                     /locus_tag="ACG8E6_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5062293.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTVTLFFPIL
                     NFYNEKR"
     gene            complement(7881..8555)
                     /locus_tag="ACG8E6_001886"
     CDS             complement(7881..8555)
                     /locus_tag="ACG8E6_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5062294.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8739..9632)
                     /locus_tag="ACG8E6_001887"
     CDS             complement(8739..9632)
                     /locus_tag="ACG8E6_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5062295.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9706..11403)
                     /locus_tag="ACG8E6_001888"
     CDS             complement(9706..11403)
                     /locus_tag="ACG8E6_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5062296.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11396..12709)
                     /locus_tag="ACG8E6_001889"
     CDS             complement(11396..12709)
                     /locus_tag="ACG8E6_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS5062297.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12709..16978)
                     /locus_tag="ACG8E6_001890"
                     /pseudo
     CDS             complement(12709..16978)
                     /locus_tag="ACG8E6_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333417.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
BASE COUNT         4813 a         3846 c         2954 g         5480 t
ORIGIN      
        1 ctttttattt tatcaaatca ctttgttttt ttataaaatt tctgcgccta gtgtggactc
       61 aaaatgtctc aatgcccatt catgtccaac gggatcgaaa cttgccacgg catctttgaa
      121 aatatggagt ttgtatccta gattatacgc atcgactgct gtatgcaaca cacagatatc
      181 tgtacatacg ccggtcaaat aaatatctgt gatctggcgt tctcgcaaac ggatgtctag
      241 atctgttcca ctaaaagcgg aataatgccg tttgtctatc caataaacat tttcctcttg
      301 ctcatagtct tggtataaac tttttaaaga gccgtacaat tcccgtccac ccgttcctat
      361 cacattatgc ggaggaaata agcggttctc cggatgaaag gaatcttttg gatcatgcgc
      421 atcaatggca aaaacgacaa aatccttttg atcaataaac ttctttgtat gagcagtcag
      481 ctttttttca attacttgtc ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa
      541 atcatatgtg tagtcaatcg agatcaatgc gttcatctta gtcctcctta tacgagatgt
      601 ttgacaaatt cgatcaccat ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca
      661 aagctttgtg tcgccgagtg atctgctgtg tcgctcattg cacgtacgat caaaaatggg
      721 atattgaact gacgagctgt ttgtccaaca gcggctcctt ccatttcgca agctaatgct
      781 tcagggaaat tcgttaagat ttctttgatt ttatctggcg agtcgacaaa tgtatcacct
      841 gtaacgatca atccttcttt tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca
      901 ctgcgcaaat atgtgcttgc ttcataatat agaggcattc ctggtaattg tcctgggttg
      961 tagccaaatc ctgttgcatc tgcatcaaaa taagctactt tgtctgatat aacgatgtca
     1021 cctacttgta agccttcacc gattccgcca gctgaacctg tattgatcac catgttcacg
     1081 ccgtattgtt ggatcaacag acttgtggta atagatgcta agacttttcc tataccggaa
     1141 cgtacgacga tcacttcgtg gttacctaat gaacctgaaa taaataaagc gcctgcgcgt
     1201 tcccatgata atggttcgct taggttctct ctcaaaattt ttacttcttc ttccattgcg
     1261 ccgataatac cgattttcat ctttttaatt ccttctttct atataaatgc gattgctaaa
     1321 aaaaccacac ataacattag gataacaata ataagagatt tcatcaaaaa agagttccat
     1381 ttggtcgtct tttcccgttc gctgatcctt gttttcgtga ttggtttatt ctttttgctt
     1441 tctttacgat aaaagctggc aatctttttt tcttcttgct gataagctgt ttctgctttt
     1501 cgctgttttt ttaatgactc gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc
     1561 gtaataagcg gtcctttggc cataagccga tccctctact ttcctttggt caatagttcc
     1621 cagtattgaa tcgcataaat cgtctttgca tcacaaatca actgatcttt catcgcttgt
     1681 tgcgcctctt caagagttag atgatacaac tccagtactt cgtcttcatc ttgtgctaaa
     1741 ggattctcga ccttctctac cccttgtgca tgataaatat gaaggacttc atttgcaaaa
     1801 cctggtgaaa gatacatcga agtaagatgg ctcagcgact tcgcacgata gccagtttct
     1861 tcttctaatt ctctagctgc tgtcatttcg ggattttgtc cttctcctgg atcgattttt
     1921 ccagctggaa tctccaaaat cactttttct aaaggttttc gaaattgttt gacaagaagc
     1981 agacgatctt gttcgtcaaa agcaatgatc ccgacaccgc caggatgaaa taccagttct
     2041 ctcttcgaag ttcctccatc tggcaaacga acttcgtcta cagcaacatc aataattttt
     2101 ccgctataga tttctttgcg actgattgtt ttttcttcaa attgctcatt gttcagcata
     2161 tcttctcctg ctttctttgt aatcataact gctttttttt catttttcaa tttttctcaa
     2221 aaactcctta ttagtttacc atagataaac cttagatttt ctctaaatat agaaaatcct
     2281 tctttagtcc gatactcttt atagtagtcc gtatgctaaa ataagtatgt aatcgaataa
     2341 cgaaaagaat tcaatactca acgataaatg acatggaggg tttattatga taaaacgaaa
     2401 ttggccgtgg cttctagcat tggtcttgct atttctcttt gtccgaaaca atagtttttc
     2461 ttttgttcta ggacttttat tgataggtgt agttttagtt ttgtggggac tctttggttc
     2521 gcgcaggaaa aagggaaaac aagaagaaat tcctgcactg actgacgaac ttgaaactca
     2581 ctatgaaaaa agcggtatga cagcgaatga aatttcattt tttcgacaaa caatgaatca
     2641 aacaaaaaat gagatcaatc aattgcaaat gaatatgcag cagacagcca aacttaaagc
     2701 aatcgatctc cgccatgatc cagtcaaagc agcgaaagct ttattcaaag aattggtgaa
     2761 agaaccaaac cgactgcatg aagcaagtca gtttctttat acgcatctgc cgaatctagt
     2821 tgatttgaca aataagtata atgagatcaa tgaccatgag atcaaaaaca aacagaccta
     2881 tgaaaaacta gaagaaagcg cgcagatcat cgaccagctg tcagctttaa tcgcacaaga
     2941 ttatcaaaaa ttcgttgctg aggatctaga tgatatcgac gtagagatat ctgttgcgaa
     3001 acagagctta aagcgtgata ataaatatga accagaacaa aaggattaac tcttttgttc
     3061 ataaaagaag actcggaaca ttcagatgat gtctggctgt ttcgagtact ttttctaaag
     3121 tagagacatt catgtaagaa tcgagaacag attcaacaag gaggaatttt ttatggatca
     3181 taaaccgact gaaacaaaag atgttacacc agttaacgat acacttgaag atttattgaa
     3241 caacccgttt tcaactccaa cagatacgct gaccacttca caacaagcgg agattgatca
     3301 gctccaagag caacagacag ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga
     3361 acaagctaaa cagctagcag caaaaatcga tgctaatgat gcacagtctg tcatcagcta
     3421 cggatcggct gcccaagcaa aattaagtga attctcacaa tcgatgttga atcatgtgca
     3481 agcacaagat attggtcctg taggagattc cttaactgaa ctgatgtatc gtctccaaga
     3541 agccaatcct gacgaactgc gtgccggaga agggaacatc ttccagcgtg tcttcgggaa
     3601 ggtcaaacaa tcgatctatg aagtgacagc aaaatatcaa aagattggtg cacaaattga
     3661 caagatttca gtgaaattag ataaagaaaa agacggtctg ctaaaagata atctgatgtt
     3721 agaacagctt taccagaaaa acaaagacta ctttgatgca ctgaatattt atatcgctgc
     3781 tggagaattg aaaatggaag aacttcaaac aacgatcatt ccagaagcga tgaaaagagc
     3841 agaagaaact ggcgatcaga tggatgttca gattgccaat gattacacgc aattcctaga
     3901 tcgtctggat aaacgtacgc atgatcttcg cttagctaga cagatcacga tccagcaagc
     3961 accacaaatc cgtttgatcc aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc
     4021 tatcgctaca gccattccgc tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg
     4081 tcaaaaagat gcagtaactg ctcaacgcca agtctctgaa acaacaaacg acttattgaa
     4141 gaaaaattca gaaatgctga aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg
     4201 aatcgtggat atcgagaccc ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga
     4261 gacattgcgt atccaaaaag aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg
     4321 acacatggaa gaagatctga aacaaaaact gttggattta acccaataga tcacctttcg
     4381 cttgttacta aaacaaacgc ccaacaacag tttattacaa ctgttgatgg gcgtttttct
     4441 tatctttctt ctaacttctc cattggagtc tatttctcat tcatcttgct gtgtccatag
     4501 ccatagccaa aataaatgat cagtccaata accattacga taccgaacac gatccatgtc
     4561 acagtttgca gacgaagcat caatacaata caagtcaaga tggagacgat tggcaatagc
     4621 ggaacgaatg gcactttgaa ttctccagct tttggtttgc caaacatttt tctcaacttc
     4681 agtaagccta gactaacaag catcaaatag gcgagcgcca tgatattcgt tagttccgct
     4741 aatacattca atgggaaaaa tccggcaaag aatacagctg ccacaccagc ggcataagtc
     4801 gcttttttcg gtgtacgatg cttttgatcg atttctgtca taaacttagg aagtaagcca
     4861 tccttgctga cagcaaacag taaacgagca agtgaataca tcatcgagat cgttaccgtc
     4921 agtagcgtta aaattgcccc tacagcgatc accgcgccca ctgttccatg tcctacatac
     4981 ctcattgcaa aagccactgg gtcattgact cctagttcag tatacggaac gattcctgtc
     5041 aagatcagcg tcacgatgat gtaaaggaca gtagcaatca gtatcgaacc aatgattcct
     5101 cttggcacat ctttttgcgg gtttttgact tcctcggctg ccattgcaac ggcatcaaat
     5161 cccaagaaag caaaaaacac aagagctgca ccatctagca caccggaaaa accaaaaggc
     5221 atgaatggct gccagttatc aggtttaacg aaaaatattc ctacaatgat aaacaacgcg
     5281 atgataccaa atttgacata taccatggca ttattcaaac gtaatgcttt cttggcttca
     5341 agcgacacga tgtaagtgac gaatatcact acgagcaccg caatcaagtc aatgtatgta
     5401 ccattttccg gattgtaccc gccagttaat gcttgtggaa gttcggcccc taaacttttc
     5461 aaaaaacctt gcatatagcc agaccatccg gaagcaacag aggaaaccgc tagtaaaaat
     5521 tccccgatca acaaccaccc tgtcagccag gcagcaaatt caccaaatac aacatacaga
     5581 taggcatatg gcccaccaat cactggcact cgcgaagcaa attctgcgaa acttagtcca
     5641 gacaaaatga caacgattgc agctaagaca aatgaaagtg acagtgctgg accagcattt
     5701 tgattggccg caacccctgt caccacaaat attcccgtac cgatgatcgc accaattcca
     5761 agcatgatca aatcagacgt ttttagttct tttttcatct cactcgtatg attgagattg
     5821 atctctttct tacgaaacaa atccattgaa gttcctcccc attttaggtt ggaaaacagc
     5881 gattaaactg ttttcatata ccctaaaagg ataccaccta aaatgactaa aaggcaacca
     5941 acaataacat aaatgagctc ttttttcgtt tttctttcgc caaggatgaa aatcccacct
     6001 aaagtagaga taatgatccc catttgtgaa agtgagaagc caacagctaa accaagtgat
     6061 ttgactgtta gaagcatgca gatgtttcct aagccccata ataatccaca gatcatgttt
     6121 ttccacacaa actgatcgac tttaacttta cggaaagcaa agaagcttgc gcctaagatc
     6181 attccgatac tttgaggtaa gataattgac atcgcatcca aattcgccca gttgacgatg
     6241 atggtatata caccataacc aatcgtagag aagatcaatg ctctaaaacc cttaccaaaa
     6301 tcaagcattt tattatctgt atctattttt ccttcgtcat cttgtcgggc agtcaaatac
     6361 gcaccgctta caagaagagc taaagcaata ataccgtata cataatcttt cgtttgtgtc
     6421 cattcatgga agaaaacggc tcccgcaatc gtattcaaaa ttaattgaaa accagtagac
     6481 aatggtagcc ctacagaaac acccatgtac ttcattccat ggaactgtcc attttgtcca
     6541 acactccaag ctaagccgga taaaaatccg atcaagaaaa tccaaggggt gattacagga
     6601 ctaacaacga aaaagacaac taaagaaaac agaaatgcac cgatcgtcat tcctagtgtt
     6661 tgttgattgg catcgccacc aattttacca cttactaaac caatactacc ccatgccaac
     6721 atagggacta atgctattaa aatttccata accaatctcc ttttatttca catgataaga
     6781 aacttatcag cgaaaacgtt aacaacacaa gcactttttt tcgtttctaa cgattttcta
     6841 acgtttttca ttataaaaat ttaagattgg gaaaaataaa gtcactgttg tcccttttcc
     6901 ttcagttgat tcagccgtca aatggatacc taatttttcg gccagatttt ttgctaaata
     6961 aagtcctaat cctgttgaat gtgttttgct aagtcgacca tttctcccag taaatccctt
     7021 atcaaagatc cgtttcaagt cttccgctgg gataccgatc cctgaatctt taactgaaag
     7081 gctgactcct tgcgcttcct tttctatgct cacaatgatg tttccgtgat ccggcgtata
     7141 tttgatggca ttactaagca attggttgaa gataaaagcg atccatttag catcggtcaa
     7201 aacagtatga tcctcaccaa cgatctcgta gcgaatattt ttttggataa aataatttgc
     7261 ctgactgcgt atcacaggct gaatgattcc ttttagtcca tattcttgga tgaggtagtc
     7321 ccgcgagaaa ctgtcgagtc ttgcataata caaaacttgt tctacgtatt catcgatttt
     7381 tgataattca ttttctagta atgtatactt ttgatcgtca atatcgaact cgatcgaacg
     7441 aactagcaat ttagaagcgg aaagtggcac tttgatttca tggacccatg agtcaatata
     7501 atctttttgg tcttgttgat tactgattgc tgattgcatt acttcttggt gttcacggat
     7561 cagctgattg atatattctt ctaccagttt ttcttcttct gtcctcccac cactcaaata
     7621 attttgcaaa ggggaagttt tttgtatgtc ggctaatttt ccccaccatc ttctcttgct
     7681 caagtaacca cccagcagat aaaaaatcag aaagacgccc tcaatcaaag ctaaataagc
     7741 aattgtgctc aaatcaactt tgatgtttgg cgccagccac atcacaaaga tcgtcaatgc
     7801 aataaaaaac agccatccca agatcaatgt ccactgatct ttcaaatatt taaaaaaatt
     7861 catcttaacc gctcatcctt tcagggaacg atatatccct gtcctacttt tgtttcgata
     7921 tatccttgga tacctgcctg ttcgattttt cggcgtaacc gattgatgtt gactgtcaat
     7981 gtattgtcat ctacaaatcg ttcgtcatcc cacagtgctc gcagcaattt ttcccgactt
     8041 aagatctttc cattgtgctt catcaaaata aataatagac gatattcatt tttgctcaaa
     8101 tcaatgatct ctccgttgat ttccatactt ccgttgtcaa cattcaacgt aatgccattg
     8161 tggctcatca tttcactaga taattctgtg tagttgtaag aacggcgaag taatgcattg
     8221 atttttgcaa tcagtacatc gatttggaaa tgttttgtca caaaatcatc tgcccccatg
     8281 ttcatagcca tgatcatatc catattcgta ttcctgctgg agataaaaat gatcggtact
     8341 ttcgatactt cacgtatttt ttgacaccaa taatagccat ccaaaacggg aagattaata
     8401 tctagtaata caagctgtgg ttcttctctt tcaaagtctg agaacacttg attgaaatcg
     8461 gtcgttccaa aggtttcaaa ttgccatttc tcaagttcct ccatgatcaa ttgtcgaatg
     8521 atctcttcgt cttcgactac catgattttt gccataaaaa atccctccat ctttcttctt
     8581 gctactacaa tatcaaagaa gcccttgtga aacaagttgg cttacctgga aaatgtcatt
     8641 ttagcaaacg ttaaaaaaaa ctggaaatat gcgatttaat cgcgtttgtc tcttccaagc
     8701 agcttaaatt tattatgtta ttcttaataa ttggtctatt atctgttatt tttctttcgt
     8761 tgtttttgtc ttttaaattt tcgataacac caataaccga aaagcgttag cactagcaat
     8821 atacctagaa ggagtataag taatcgataa agatggtatt tttttgtctg ttctatcttt
     8881 gaagagactt cttctgtaaa gggaactcgt cttcctgtca ctagtaatcg gtgagtgttc
     8941 acggtgtaag gtgtacaagt gataagtgtg actagatcct gcttttctct aattgctaat
     9001 gtatttgttt ttgttggaag gacaacaatt ttctcaacga cttcataagc gagcatccga
     9061 ccatacactt caagatagaa tcggtcatct tgctccattt cttcaagatc cgtaaagagt
     9121 tttttctcag caataccgct atgggcggta atcacggaat gtgtactctc tcctccagtt
     9181 ggaaaagagg tgccttgcag gactgtcgct cctctatcta acaaaagatc attcgtttca
     9241 tcaaataaag gcaagctaac attgattttc ggaatataga tggctccgat catatgttgt
     9301 tcgtaatatt cttttcctgg attttggaca tttctaaccg cttgctcaaa aggatctttg
     9361 acttgtccca ttcctgggat tactgatgta tgagcaagcg tctgattttt cttttcatgc
     9421 actgctaatt gttgctcatg tttttcttct ttatcgcgtt caagctgctt ttgataattc
     9481 tcgatccttc tttgatccaa ataattgctg agagcatctg ctaaaaatgg atatgtaaaa
     9541 actgcagctc cggcaagaaa catcactgcc ataaataatt gtaataaaag actgatttgg
     9601 gtttgaggtc tattacctat tttctttttc attttcttca ccttcttatt taaaaaaact
     9661 gcagcaacct ttataaaacc gattgctgca gcttattttt gttgcttata cttcagactt
     9721 cattttagat ttacggtacc agctgtacgc tccaatcatc aaacttattc cgattagtaa
     9781 gaatgccaag agcccgttcc caccagtagc aggaagtgac ccgcgtttca tattcggtac
     9841 tctttctggc cctggatttt ctactcctac gatctgaatt tcttcattat agcttccata
     9901 agagatgata aattcagtaa aggctgcctc acctgttgga agtacataac cttctggcgc
     9961 catggtctct ttcatttgat aggttccata atctaaccct tgaacctcaa agtatccatt
    10021 tccatctgcg acaaattctg ttgcttgtcc gcttgttgcc cactcaattc tatctgctcc
    10081 tgattcataa acagctaaag gcgactcccc tttataaaat attgcgtatt cagaacggtc
    10141 ttcattccac aattcgaatc ttgcatcttt aagcaattca ctgcttgatg catcatattt
    10201 ttcaaacttt cttccacctg ttgtgacttt ctctggcggc tctactcctt cttcataatc
    10261 aaattcatca tttcgtccga tttcaataac cgctgtgttg ttatggaaat catccgcttc
    10321 tagttctgtg gaaaccttca aattatacgt gatcgttaaa tctcctccac gtgcatctgc
    10381 taatctacct aaagtatcta ctttggctac tccattttct tctgtcaaaa tcgtcacgac
    10441 aaacccgttg cttactactt ctactatata atcttgtcct tttgttaaaa tcgtctcgcc
    10501 ggctcgcact tctatttcct gatcataata agttagacca actgtaggcg tatctgtgat
    10561 tttgtaattt ttgataacag cgcttccgtt ttcaaggaca gaatcgatga aataaggaat
    10621 aggtgctgtg atcgtgtaag aagccgtttt tcctctttct agatcaatat aagaaatcgt
    10681 ttcttgttct ccagcttcat taataatcgt tacttgtcta aggtcgcttt cctctacatt
    10741 catctgttta tctgcttctc tgatttcgtt tttcggatac aaatgcacat ctgtattgta
    10801 tgtgtctttc catgtagaac catcccacac gccatccacc atatcaggca tcatcactgg
    10861 catcataaca accattggag acgcgattct ggaaatatga gctggcgaat aagtttctaa
    10921 gaaaagatag actttatctc tagaagaagg tgcttcttcc gtcacttgga cggtagagaa
    10981 taccgcttct ccagcttcat tcgttgtttg tttgtcaata cttgtcagtc gatattttaa
    11041 aaaccaactt tctgtgtgcc gtttttgaat ccattcaatc gcttcggcag aattaagacc
    11101 gtcttctcgt tccggtgtta aaggattatc tcgaatcaaa tcgtaatata cttccgttac
    11161 gtcaaacaaa tcaaaatcga ctcctggaat attttcttca ccaaaatccg tagaaaccaa
    11221 tccgctattc ggtcgttctt ccggaacaga tgagaaacct tttttgtgta atgtaatcgt
    11281 cacttcactt ggtttttgtg cgacttcttc ggctttgact agttttccgt tcccgaaaat
    11341 ccctattccc gcagtaacga gtaacagtac actcattccc gtgacaatca tttttttatt
    11401 catttttttc tccccgttct ttttttatta ataaaacagc acctaacaat aaaagaattc
    11461 ctagtaaaga aatcaacgtt cttgcttcat tcgtttgcgg gaatacggac cattttcctg
    11521 attgacctgg attgcttgga ccactcggct ctttcggttt gtccgttgtt tcttttacgg
    11581 tctcgttata aacacgtggt gttgcggatt cataggcaga gactggaact ttcccggatt
    11641 ctaattcagc catctcctgt ccgttaacta ctacatattg tggttggcct tttgcatcca
    11701 cccaaaattc agggatgatg acctcgattc tcttacttgc ttcatccatc tcgtaacctt
    11761 ccacaccttt taattcttca aaataatagg tgccagaagg caagaaacgt tgtcctgtcg
    11821 taacaaggcc ctctttatcc gaaatgaatt tgcttacatt tttgtcattc aaaggatcat
    11881 tgtctgctgg tttgatccat tgatttttca agtcattagc aggagccata tctaaataat
    11941 atttcccccc ttctatcatc tgatatagag caaaaacagc accttctaat ggttttcctt
    12001 tttcagttgt tcctctttga cgtccatact tgaagaagta tggatctctt agataaccga
    12061 catttttagg atagatatga atctcttgca gttcctcggt tgattctgtc gggtgataaa
    12121 ttggcaaaat agcagcaatt gggatagctg tctgatcgat atcaacatcc aatccaactt
    12181 ctggatcaat cgattcttcg aaaatcaaat aaactgcatc ccgatcttgg tttttcttag
    12241 gtgctttgat acgtgcaatc ccgtctccta atccgccaac cgtatctttc ccagttgtta
    12301 ccgaagtccc tatttggaca agcctttctt ctgtgattag ctgacggata ttgtttctgt
    12361 tcgtttcagc gatttgctgg acgaattctt taggagtcat gttttcagca gctttttttt
    12421 catgataata atcggtcatg tcgtaaatgg caaatgtcgc tccatttagt ggcaccgtct
    12481 gtgaaatgat atctgctgtt tcccctgctt cctcagagat agccaagcca tcattttcat
    12541 ggtattcaaa ttgatcgtta taatcgatat ctcgaatcat ccgtttatgc aacacgaatt
    12601 ccacgacatc ttcccctgat tcttctgccg acatcgcatg tggaagaagg actgctgcaa
    12661 gcggtagtaa aacaagtaat tgaagcatca aacacaacag ctttttcatt accttgctcc
    12721 ctccttttta gattgtctga caaacaaacc ataggctgct cctgcaagcg ccatgaagaa
    12781 tgcagcaagc aggatataga ataatcgtcc ggatcctcct gtagcaggca acggattttc
    12841 aggatcgttc ccgacactaa gctgtaatct gattcgattt gtctccaaaa gttcatattc
    12901 taactcttgc tgtagatcag gcgtctctgc ttcttcgttt ggataatacg aaatctcaac
    12961 ttttagtgta cccgcttctt ttcctggaac tacccgatca ttttctatgt caggttcttc
    13021 tcgataacta atttctaaaa taaagtaacc tggaagtttt ctatagctgt caggtgactt
    13081 cacttccttg atccgataga ccccttccaa caagtctgaa gcattgaata gggaagaatc
    13141 gtttggatct ggtgccaagc ctccctcgat gatttcatac tctcctgcac tattttgttt
    13201 ctcaattgaa aattctgcgc cttcaagacg tcggctattc atactgtcaa ttttcaaaag
    13261 ttccaaatcg attggcgcta gttcatcttc taaggttact tgtagtacac catcgatgat
    13321 ttcatagcct tccggcaagg gagcagtttc gtcttcgatg accgtatctt caatgataaa
    13381 cttaccttct tcattcagtg caaactggaa cacttgatca gatagccgat acccatttgg
    13441 tgcgacagat tccgagacct catattcacc aatagacaga tgcagaggtt ggtcgtaatt
    13501 taaatcgaca tacttttcta ctcctacggc acgagaatag aagtatccga tcccaggcaa
    13561 acgtttcgat tcttctggtt ctaacggatt accagtgtat gctcgataca gcgggaagga
    13621 agaatccccc actttcctga tatcgaaacc tgcaccatct aatgtatggc cttcatggtc
    13681 taattttcta actcggatct cccgataatc aaacgtattg taagcagtga atgctaccca
    13741 taaacgatcc tcttcatctg ttgtatacgt gagatattgc caattttctt cacctgtttg
    13801 cttgagttgg atagtcccta tagcattatc gtaaagtcgc aagatccatg tgtcattttc
    13861 atcaggttct ttgaaacctt ctgggttctg gctctcaatg attttgtatt ctgcatagtt
    13921 ggtagtttct tcactatcct tataaacaaa tgaatctggc tctccgattg gatgaataac
    13981 tttctcacca tccacgatta catatttccc gtcttcatct actgaataga acgtgagtct
    14041 ccctttctct tcctctactt cgttcacacc agaaagccct ttcgctactg gatatccgat
    14101 ttccagtccg ttaccatctg tttcataaag ttcaaactct gcgccattga ttggcatatt
    14161 actgccttca attagtttat tgatattcat gtggattggc ttgaattcat ttcgcatttc
    14221 cagtttgaca tacaactcat catactctgt gccttctatg ttaggctctt gtacaacaga
    14281 aagcttgtga tattctcctt cataatagga attcggatct ttaccttccg gtccgccgac
    14341 tctcgaacca gatgtatatt catctacaag ctcgtagtga cctaacatct catcttcgtt
    14401 ggctgcatat tttttgattt ggaattgata cgtctcatct actaactgat aacgtggtgg
    14461 tccagcgatc tctctgatcc aaaatatgtc gctgactgct ccagtttcat ctcctaaaat
    14521 cggcatcttc gtttcaaaga tcgctattcc gtccgtggta gttttacttg tttcgattgg
    14581 ttcggcttcc ccactctctg gattttggtc atataatgca aattcgactc cagccaacgg
    14641 ctcgccattt tgacccttct tgctgacttg gaaattacgg cctttatgtt cgtaacgaat
    14701 ggaagaagat gggaaataca tccgtccagt agtgatatca ttctcataat cctccaggtt
    14761 ctgaatatag gccatattgt tcactgtgca aaaatttccg tcttgagctc ccgcatcaag
    14821 ttttacttgg tacttgatcc tgaccatttc cccttcacct aaagaaatcg gagaaaattt
    14881 ataagcttgg ttttcccgtc cgacatacgt tgtcggagtc gccactactt ctggtggggc
    14941 ttgtccatga cgttcttcat cccatatttc tggctcttgg tacttcatgc cgttttctcc
    15001 tggatctaga tggtaaaact gaatatctgc cgatctgcca gctccaaatg gtgagacatt
    15061 aaaaccagtt tcatcataaa gtgtggcttg ctggatcgtt ctcttgaagg tagaagagat
    15121 atcctccaag gctgtaacaa tgtcattctt gttgttcgca ttgtaatatc gcggtgttac
    15181 gccatcttct tctaaatcag caatattttt caatacattt cgtccaatag catctagtcg
    15241 ttgtctcccg ctaacgtttc catcgattcc taaaccaatg gagaagatac ttggcgggtt
    15301 ctcttgatac ttgtttttca gccaatgatg ataagcaact gtgttgatcg cagatgactt
    15361 ttcttgcgaa gtaggactgc cattcgtatt gttattagaa gaaccatcac caacccagtg
    15421 agtcgcttta ttatctcctt cttttacctc tccataacga tagtgtacat ttacttcatc
    15481 ttctggacgg ttaaagtcag atgcataggt aactggatga gtaaaccctg cattagaagt
    15541 attcccagaa aactcctcaa aatttctgaa caaaacgtca ttatcttcag caatcttatc
    15601 ttccatcaca gtccagttgc tccaagctcc attagtgctc gtacgataac ttgactgaat
    15661 cggtgcataa ctgaaggtcg gtgtcccatc tcccacaacg attaggattt tttctggatt
    15721 tctattttct ccgccattgt ctttatacaa tgtttcatag ccatttttaa gccctagtgt
    15781 aagcggagtt cctccgattg gtgtacgatt taatgctgta ttctcacttc ccgtcaaacg
    15841 attgatatca tttgtcaatg gaaagatgtc cgaactgatt tgctcatggt tattgatagc
    15901 tgtactatta tggtaaaaat tcaccatacc gattcgaata tccagactcg ggtcagagag
    15961 cagatcttta ctcatttcgt ttactgcctc aatcaacgca gcatttttag tttgactatt
    16021 ggtcccagcg gttaattcac tcatactcgc tgacttatct agaacaaaaa cgatatcgat
    16081 tggactgatt tcctttttct cgcccccgat gacatctaga tagacattgt aaagggttgt
    16141 tgcatcttca ggatgtagat cagatgctgt gctgcttggc agaacaggtt ctacccattt
    16201 tttgatgtac gccccgttat aatccaagta actgttttca aaattccgtt catttcccca
    16261 aagctgaacg gtttgagctg gaacgtttct atcttcttcg cctgcagttg atccattatt
    16321 agtagcatag tcgtaatttc gccagttcgc tcccccgttt gtataatcac tatcatcgat
    16381 caagttctct ccgtcatatg gcgagccttt gtctccttga tagcgttcat tgctttgtgt
    16441 ccaatttcct aacacttttg tatagtcatt cgttgtaaac ttcatctatt ctttcatcaa
    16501 ccaccacttg aagaggtagt tacccttcgt tcacatcggg gatcttttct gtttcaaaaa
    16561 tcaatgtgcc ggtttctgct tcttttgaat aaacttttcc gacataccat tcttgaccat
    16621 cgatcgtctc gacttttagg tcttctccat cttcatcata ggaggataga cctgttccac
    16681 gaacgttttt tacggtacca atcccggcgg ctgccgcatc gattgctaaa cgtattctac
    16741 cttcattggc actttccttt ttttcaaaag ccagatgcca ttcaagggtt tcttcttttt
    16801 caatatactt cccttcaatt tttgcatagt cctcatctaa gaatacctca tcctcctttg
    16861 tttctgttga ctcagcagct acatccaggg tcgtcatatg atagactgac tggaaatatg
    16921 gtccaaatag caaactgaca gttactaact gcaaaagctt caattttaac cctttcacat
    16981 ccttatactc ctttcaaaaa tatttgtctt ctttcacaac aatcaacaaa acaatgccca
    17041 tctaattata atttaattta attttaattt taaatcaaat gatatccttt tta
//
LOCUS       DAZUMM010000058        17007 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000058 DAZUMM010000000
VERSION     DAZUMM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.058"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..126)
                     /locus_tag="ACG8E6_001891"
     CDS             complement(<1..126)
                     /locus_tag="ACG8E6_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFS5062298.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(230..541)
                     /locus_tag="ACG8E6_001892"
     CDS             complement(230..541)
                     /locus_tag="ACG8E6_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS5062299.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(543..740)
                     /locus_tag="ACG8E6_001893"
     CDS             complement(543..740)
                     /locus_tag="ACG8E6_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS5062300.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1134..2861)
                     /locus_tag="ACG8E6_001894"
     CDS             complement(1134..2861)
                     /locus_tag="ACG8E6_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFS5062301.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2854..4047)
                     /locus_tag="ACG8E6_001895"
     CDS             complement(2854..4047)
                     /locus_tag="ACG8E6_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5062302.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4234..4878)
                     /locus_tag="ACG8E6_001896"
     CDS             complement(4234..4878)
                     /locus_tag="ACG8E6_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5062303.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4972..5106)
                     /locus_tag="ACG8E6_001897"
     CDS             complement(4972..5106)
                     /locus_tag="ACG8E6_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFS5062304.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="ACG8E6_001898"
     CDS             complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="ACG8E6_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFS5062305.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7237..7710)
                     /locus_tag="ACG8E6_001899"
     CDS             complement(7237..7710)
                     /locus_tag="ACG8E6_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFS5062306.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8111..8335
                     /gene="nrdH"
                     /locus_tag="ACG8E6_001900"
     CDS             8111..8335
                     /gene="nrdH"
                     /locus_tag="ACG8E6_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFS5062307.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8494..10653
                     /gene="nrdE"
                     /locus_tag="ACG8E6_001901"
     CDS             8494..10653
                     /gene="nrdE"
                     /locus_tag="ACG8E6_001901"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFS5062308.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10703..11668
                     /gene="nrdF"
                     /locus_tag="ACG8E6_001902"
     CDS             10703..11668
                     /gene="nrdF"
                     /locus_tag="ACG8E6_001902"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFS5062309.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11757..12896)
                     /locus_tag="ACG8E6_001903"
     CDS             complement(11757..12896)
                     /locus_tag="ACG8E6_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5062310.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13205..13918)
                     /locus_tag="ACG8E6_001904"
     CDS             complement(13205..13918)
                     /locus_tag="ACG8E6_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFS5062311.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="ACG8E6_001905"
     CDS             complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="ACG8E6_001905"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFS5062312.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15107..16639
                     /locus_tag="ACG8E6_001906"
     CDS             15107..16639
                     /locus_tag="ACG8E6_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5062313.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16830..>17007)
                     /locus_tag="ACG8E6_001907"
     CDS             complement(16830..>17007)
                     /locus_tag="ACG8E6_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5062314.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2910 g         5324 t
ORIGIN      
        1 tgaaaaaaca cattccacta aatttgatgc cggttttcct tgaggttttg ttccagcacc
       61 tcctttaatt tttttaagct cttgatctga acattgttta gcatttagtc tatttttttc
      121 ttccattacg attcccccta caaactataa atacttttat gtcactgatt caatatacct
      181 atttttctaa tcaaaaaatt ttgagacata tttaatatta taagtatcat taaaattgag
      241 atttatctcc ataatctgct cgatataaac catttattcc agcagttctt ccaatttcac
      301 tcaaatttct tattaattcc tcttgatatt cagtgaatct tattttatta gtataagcag
      361 taaaatatag ataattcacc aatctattta ttaaaggact gggatctaat tcatattttt
      421 gtttttctaa ttttaaatat accttttgta aaacctcaag aatatcagaa tatttaggat
      481 ctttacttat agatatatca ttatataatt catgaacaat ttgcttagca ttttttttca
      541 ttttagcact tccctggaat tgctccacct aaaaaaccac ctatagacat tcctgcaata
      601 caagtagttg ccttggccca atcgaccgta catttatttt tagtgcaata cactccattt
      661 ccataatatt ttccactatg agtggtaccc ccatagataa gttgtgtctc tttaatagac
      721 aaaattttta aatgtttcat aatctaatca cccctaaatt aatgttttta gactattatt
      781 ctaacattca ttaaatttca aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg
      841 aataagaatt taatcacttt taagtatata aactatgtat attctttaat attttttgag
      901 aatgcacttc gtacataata aactttaatc acattagtct ttcataccta cgatcgatct
      961 ctttgactcg cacccataaa ttcataatag tttgtcggcg tctttgaacg tttcgaagat
     1021 gagctacgca ttctcttttt ttatagacaa aaaagataga gacgaacatg gagcttccaa
     1081 atttggatat ttgatccaat tttggaagct ccatttattt cgtgtctgtc tttttatttt
     1141 ttccagcttt cgactaattc ttggttctct ttgatccagt cttttgctgc ttgctgtgga
     1201 tcttttccat tgttgatttc caacataact ttttccatat ctttttctga ccaatggaaa
     1261 ttgtctaata ctttgtaagc ttctggctgg tcttctttca atccttttcg aaccatcgta
     1321 tggatctgct cttcgcttcc cattccgttt tcaggatctt ctaagtattt cagatcatat
     1381 ttggcaaaca tccaatgagg tgtccatcct gtaactacaa ttggttcgtg ttttttgatc
     1441 gcttgaccta gtgcaacggt cattgctcct gaagaagatg tctctacttt ccagtctttc
     1501 aagttgtcat atttttggat cgtgttttca gcagccgtaa ctacaccagc tcctggctcg
     1561 attcctatga ttttcttacc tgcctgatcg gttaactcat cgatcgaatt tacatccatg
     1621 taggctggca ctgctaaacc aacttttgct cctgtcaaat tggcacctag gtcttcgact
     1681 tgatctttat attgtgcata ttgtgaacca tgagttttag gtagccaagc tgaaaccatg
     1741 gcatctgact cgccatttga aacagatttc cacatgattg aattatctaa tggcgtcatc
     1801 gttacatcat agcccatttg tttcaatact tcaccgacta catttgttga agcaacttct
     1861 gtatcccatt caacataaga taagttgata cgttttgttt ctttggcaga tgaaaaactc
     1921 cctataccaa tcgcacctaa gatcactact actacagcac caatgatcag tccttgacgt
     1981 tttttacttt tctgtgttgt tgctcctgca gcttttttat tgtttggctg gttcaaatgc
     2041 tgtgtaaaac ggtcaatgat gattgctaag ataactaatg caaccccgtt gacaaatcca
     2101 ttcccgactt gtgctctttg caatgctgat aatacgccgc gtcctaagcc gggtgcaccg
     2161 atcatagagg caataacgac cattgaaagt gacagcattg ttgtttgatt gatccctgcc
     2221 atgatcgtac ttttagctaa aggcaattct agtttgaata gtttttgacg acttgtgctc
     2281 ccgaatgaat cagaagcttc tactaattct ttaggaactt gtcgaatccc taaattcgta
     2341 aaacgaacgg ttggtggcag agcaaagatg actgaggcaa atacccctgg aaccatcccg
     2401 ataccgaaaa atgcaactgc tggaatcaaa taaacgaatc ctggcattgt ctgcataaag
     2461 tcaaggatcg gtttgataat actttgggct ttactgcttt ttgccatcaa gatccctaat
     2521 ggtaccccaa tgatgatcga gatcacacta gataaaagaa caagtgtgac agtattcatc
     2581 aaatcattcc atagaccttg gttataaata aaccatagac cgattaatgt gaacaacgta
     2641 agtcctggac gtttcttaga aataaagaaa gctgctaccg tcaataacaa gataaacaaa
     2701 ggagctggaa tcactaacag taaatttgta atccccgaca tcactgcctg tccaatcgtt
     2761 tgtaaaaatg aaaataggcc agaaaaagtt gtcgtaaacc attctgtgat attttctacc
     2821 cagtttgcca ctggtaattg atagttcaat agattattca tgttcactca cctcgcttgt
     2881 ttctgctaat gcttcaatca cactacctcg gatgacgaca ccttttaatt ttccatcctc
     2941 tacaactgca agtggagtcg gtgagtcata aataatattg aaaatatcac ttaccaccat
     3001 gtctttccca atcaccgtca cattttcatc taggaagtcg atcaatggac gtttttgctt
     3061 cctagcttct aacgcttgat ctgcagtgat gatccctttc aggtgtctct ttctgtcaac
     3121 agccatcagc atgctgactt cttcattacg catccttgtc aatgcaacgt ttggtccatc
     3181 agattcgata ttcgtcgtca atgcaggtac catgatgttt tgagccgtaa gcactttgga
     3241 acgatcgact tcttccacaa attcacgtac ataatcattt gctggattgg tcaatatttc
     3301 ttcacctgtt ccgatctgca tgatttcgcc gtctttcatc aaggcgattc tgtccccaat
     3361 ccgtaaagct tcattcaaat catgggtaat gaaaataatc gttttttgaa cattggcttg
     3421 caggtccagt aattcgtctt gcatttctcg ccggatcagc ggatcaagag ccgagaacgc
     3481 ttcgtccatc agcaagatct caggatcatt tgctaaagct ctggctaggc caacccgttg
     3541 ttgcatccca ccagataact gactaggata ttggtcttta aatgaaagca aactagaatt
     3601 ttctaatgct ttttctgctt tttcttgtcg ttcttcttta ggtacaccac ggacttctaa
     3661 cccgtattct gtattttcca agatcgttcg atgtggaaac agaccgaagt tttggaaaac
     3721 catattgatt ttatgacgac ggacttctcg caattcttct ttgcttaact tcgatacatc
     3781 ttctccatca atatagatac tgcccgaagt tggctcaatc agacgattca atagacggat
     3841 caatgtggat ttcccactcc cggaaagccc catgatcacg aagatttctc cttcttttac
     3901 gtcaaaattc acgtcataca caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt
     3961 cggctggtga tcattcatca tatctaaagc ggcttgtgtc tttttaccaa atatctttgt
     4021 taaatgtttt acttctactt ttaccaaaca aattctcctt ttttatgtaa attcattttt
     4081 ctttctgaaa aaagtgacca cactatgtta acatcatgta gtcactttgt caaaaatgaa
     4141 agaaatttgc ttgtccaaac tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta
     4201 gccaagttaa tattttttta atatttcaca caatcattct gcaagctgat gctccgggta
     4261 aaaaaagttt gtcatacgct gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc
     4321 tgccgtaaat ttagcatagg ggccaggtga aacaagcagt tcttcattat gagaaattgc
     4381 gataactgtg gcgccagtcg cctgccagat attcaactcg ctaattgaac ggtccagatg
     4441 cattgctttt tctgataaag ataattcaaa cggaagtaaa ggattcatct tatgtattcg
     4501 cttcgtttga ttgaccagct gatccaataa ttgagaaaaa tgattcagct catcttgttg
     4561 cttctccaca cttgccatga tttctgcttt tgtatcttgg atggattgta catcttgata
     4621 ttgcaatagg aaatcttttg ctttttcctt cgaagcaaca aatgccccac ttccatgccg
     4681 tacttccatg attccaagat ctaccagcac attgatagcc ttgcgggtag tctcaggaga
     4741 agcattgtac gtactggcaa gtgttgagcg ggcatggatc ttctcaccca ctttatatcg
     4801 gttttcaacg atacgttcgg ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat
     4861 ttttttcttt ccagacattt tctttcctct ttcttttttt actttccctc atcttactat
     4921 gctctagcaa aataagcaaa aaaggggaga atagcctctt gcaacgactg atcatcgctt
     4981 gatctgttcc ttttttactg ggcaagctgt ttgacagtca tcacaattct taccggattt
     5041 taaacgagta tagacaattg cgcctgcact tccaaagatt aacacactta ataagaatgt
     5101 cgccatccta ttcctctcct tctaatgaca atatagtata aaactggttc ttggattttg
     5161 gctgacggaa caaaacataa acgagtccta tcatcagtaa aatagcaaga tacgtcccta
     5221 acgctacctg tccaccctcc aataagtgtc ccaactgata aatcacaaaa ctaactaaat
     5281 aagctaaacc gcattgatag cctatggcga tccaagtcca tttcttggca ttcatttccc
     5341 gatggatagc cccaattgcc gcaaaacaag gcgcacaaag taaattgaag gctaacaatg
     5401 aataagctgc tgcgggagta tatgtgtgtt gtagcacttg ccagatttct ctgccatttt
     5461 cggatacttc cgcatgacca aacaaaatac caaatgtgcc aataatattt tctttagcga
     5521 ccaatcctgt aatcgctgcg actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa
     5581 caactgccag gattcgcccg aaaaaggcta agatactttg gtcttcagaa acaggttgag
     5641 cataaaaatt ataagaagag ctgaaccaga tcacgatact agaaacaaag ataatcgttc
     5701 ctgctttttt aacaaaagac ttgctgcggt cataggtttg tcttaggacc gctgaccatt
     5761 gaggaaaatg atatgcgggc aattccatga taaaaggagc tgtatctcca gcaaacaaac
     5821 gtgttttttt caagctgatt ccagagaaaa taattgcagt cataccaaga aaataggcag
     5881 aaggagctac ccaagattga tgaggaaaaa aggcccctgc tactaaggca ataatcggca
     5941 actttgcaga acaaggcatg aaggttgtga ccatgatcgt caaacgacga tcttgctcgt
     6001 tttcaatcgt gcgactggcc atgacgcctg gcactccaca tcctgtagca atcagcatag
     6061 gaatgaagga ttttcctgaa agaccgaatt tcctgaaaag tcgatccatg acaaaagcga
     6121 tcctcgccat atatccgcag tcttccagaa agctcaagca taaaaacaat ataatcaatt
     6181 gcggtagaaa tcctaacact gctcccacgc ctgcgatgat gccgttcaag atcagctcct
     6241 gcatccatcc cgctacttgc cattcagcta acaatctgcc aacatgttct ggtaaccatg
     6301 taccaaacaa tgtgtcattg atccaatctg tccccattgt tcctactgtt tgaatagcca
     6361 gataatagat cagccacatc acaaatgcga agatcggcaa ggctaaccaa cgattcgttg
     6421 caatctgatc gattttgtca ctcatcgata attggaaaga atcatttgac acaacacaga
     6481 gtgtacatag ttgggtaata aattcataac gttcattgac cagaatcgtc tcactatcgt
     6541 cgtccaatag tttctctgtt aactggatca gttgctcgat ctctttttgc tgagaaactg
     6601 ctatctcgta atcctctctt accttctgat ctcgttcaaa atatttcatt gcataccagc
     6661 gtttcttgcc gctttgttct cctagtacat cttcaatttc atgtaaagct gcttctaaac
     6721 gtggatcata tgtacaggga acaggaatag aaggccgctt ttttgcagac tcctgaatct
     6781 tttcgattag tttttcgatt ccttgctttt tcaatacgct gattggtaaa actggtacac
     6841 caagaccata ggaaagcttt tctagattga ttttttttcc ttcttttttg atgacatcca
     6901 tcatattcag accaatgatc ataggaatgt ctaaatccaa taactgcgta gttaaataaa
     6961 gatttcgttc taaattggta gcatcaataa tgttgatgat cagttcaggc tgttcctgaa
     7021 ccaaataatc tcttgcaatc acttcttctg gtgtataagg agacaaagaa tagatgccgg
     7081 gtaggtcctg aatatgaatg ttcggatttt ttttcaacga tccttctttt ttttctactg
     7141 ttacgcccgg ccaattccct acatattgat tcgaaccagt caagagattg aacaagctag
     7201 ttttcccgct gtttggattt ccgactaacg cgatattcat tctttttcct ccctttgtat
     7261 caagattagt tcggcctctg actttcgtaa cgtcagttca taccctcgca ctgtcagttc
     7321 gattggatct cccagagggg ctagttttcg gataactatt tttacatttc gcgtcaatcc
     7381 catatccatt aaccgtcgct tcactgcgcc ttgtccgtgg attgctacga cgatcccagt
     7441 ctctcctgct tttaattgat caagagaagt ccagctttca gaggaagcag tctttttatt
     7501 gacaaagatt tgacctagta tttcccgact tactgccact cgatgacttt ttaccagtaa
     7561 aatggcattg ttgcctgata aattgacgac gacgatttta cttccggcaa cgatcccgat
     7621 attttgcaaa tggcgttttg ttttttcttc tgtttcgatt cgttcgactt gataaacgaa
     7681 attctgcttt gcctgcgcta aggtctgcat attgattcct ctcttgtgaa ataaatcatg
     7741 tattaaaatc attcattata tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat
     7801 cattctcaca tagaatactc tcggtaattg agaatgtcaa ttatacttgt gatattatga
     7861 gcgaatcttt agtattgata gataaaaatg agaaaaaaat atttcttcat agttttccac
     7921 aattttttct ctattaaatt tgctattttc ccacacgata cttgttttca gctctttttt
     7981 caaaaacaca aaacttagta ttccacagtt tccacaacac aatatgtagt gtttgttgtt
     8041 tgactttttt actaagagtt ttatactatc tattatagat aataagaatg tgttagagga
     8101 ggcactcacc atgaacgtaa aaatcttttc taaaaataat tgtatgcaat gtaagatggt
     8161 caaacggttt ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc
     8221 tgaagcagta gactggttga aagcacaagg attccaaagt gttcctgtca ttacttctga
     8281 tgcagaaaca gttatcggat tccgtcctga tcagttgcgt aaacttgcca gctaacatga
     8341 ctaaaagaat caaaacgtga tacatggatt tgaagggctt gagacagcac cagtttttgt
     8401 cgcaaacttc caaatccttt tttaaagctt aaaaacaatg caatgatttg caatcaagca
     8461 tcatcaaata cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc
     8521 agttatttca agctgaataa tgaaatcaat cggccagtca atggtcaaat ccctttgaat
     8581 aaggataaag aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact
     8641 tttccatcta ttatggaaaa aatcaattac ttgatcgatc aagaatatat agaagcagaa
     8701 ttcattcaat tgtattcacc atcatttatt gaaaagctat atcaattttt ggacgagcaa
     8761 catttttctt tcaaatcttt tatggctgct tacaaatttt attctcaata tgcgttgaaa
     8821 accaacgacg gttccgctta tctggaaact tatgaagatc gagtagcctt caatgcgctt
     8881 tactttgcga acggggatga agaattagca cttgctcttg cagacgaaat gatccatcaa
     8941 cgatatcagc cagctacacc ttctttcttg aatgctggac gtaaacgtcg gggcgaattg
     9001 gtgtcttgtt tcttgactca agtcactgac gatatgaact ctatcgggcg ctcaatcaac
     9061 tctgctttgc aattgtcccg tattggtggc ggcgtaggga ttactttatc aaatcttcgt
     9121 gaagccggcg ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg
     9181 aaattattcg aagatagttt cagctattct aatcaattag gtcagcgtca aggagctggc
     9241 gtggtttatt tgaacgtttt ccatccagac attattgctt tcctttcagc aaaaaaagaa
     9301 aatgcagatg aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa
     9361 ttttatgaat tgacacgtaa aaacgaagat atgtatttat ttagtccata tagtgtcgaa
     9421 aaagaatatg gtgtcccatt ctcttatgta aatattacag ctgagtatga tcatctagtt
     9481 gcaaatccaa acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca
     9541 aaattacaac aagaatctgg ctatccttac gtaatcaata ttgatacagc gaaccgcgag
     9601 aatccaatcg atggcaagat catcatgagt aatttatgtt cagagatttt acaagttcaa
     9661 acgccttctg tgattaacgg caaacaggaa tatgaagtat taggaacaga tatctcatgt
     9721 aacttaggct cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg
     9781 cgtacgatga ctcgtgcatt gacatttatt acagatgcat ctgatatcga tgtcgtacca
     9841 ccgatccaaa acggaaataa gctgaatcat acgattggat taggtgccat gggcttacac
     9901 acttatcttg caaaagaaca aatggaatat ggctcagagg attcattaga ctttacaaat
     9961 atttacttca tgctgttgaa ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa
    10021 agaaaacaag ttttccataa ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa
    10081 aaatacgtaa ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc
    10141 atttttatcc caactgctga agattggaat gctttgaaac aagcagtaat gaaagatgga
    10201 ctgtatcatc aaaatcgctt ggcagtagca ccaaatggct caatttcata tatcaacgat
    10261 acgagtgcaa gtattcatcc tatcactcgc atgatcgaag aacgccaaga gaagaaaatc
    10321 ggtaaaattt attacccagc gccttatcta tccaatgaga cgattccgta ttacacttct
    10381 gcttatgata tggacatgcg taaagtaatc gatgtttatg caacggcaca acaacacgtc
    10441 gatcaaggca tgagtttgac tttattcatg cgttctgaaa tcccagaagg catttatgaa
    10501 tggaagacaa caacgaagca aacgacacgt gatttgaata ttttaagaca ttacgctttc
    10561 cacaaaggaa tcaaatccat ttattacgta cgtaccttta ctgatgatgc agaagaaatc
    10621 ggaagcaatc aatgtgaaag ctgcgtgatc taatatcata gatagattcg cttattcata
    10681 ttttaattaa aggagcattc tcatgtcaga aacatattat gaagcaataa actggaatga
    10741 aattgaagat atcattgata aatccacttg ggagaagctg acagagcaat tttggctaga
    10801 tacacgtatc ccactttcaa atgacctaga tgattggcgt acattgtctc aattggaaaa
    10861 agatacagta ggacatgtat ttggcggttt gacactgcta gatactgtcc aatctgaaag
    10921 tggcatggat cagttgcgta aagatgtccg cactccccat gaagaagccg ttttgaacaa
    10981 tattcaattc atggaatccg ttcatgccaa aagttattct tctattttta gtacactgaa
    11041 tacgaaaaaa gaaatcgaag aaatttttga ctggacaaat acgaatcctt atctgcaaaa
    11101 aaaagctgag cgtatcaatg aaatctacaa acacggtaca ccgctagaaa agaaaattgc
    11161 cagcgtcttt ttagaaacat tcttgttcta ttcaggtttt tatactcctc tttattattt
    11221 aggaaacaac aaattggcta acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc
    11281 tgtacatgga acctacatcg gttacaaatt ccaattagga ttcaacgagt tacctgaagc
    11341 agaacaagaa acgttaaaag actggatgta caatcttctt tatgagcttt acgaaaacga
    11401 agaacgttat acagaagaat tatacgatcc aatcggctgg acagaagaag tcaagacatt
    11461 cctacgctat aatgccaaca aagcattgat gaacctaggg atggatccac tctttccaga
    11521 tactgccaat gatgtcaacc caatcgtaat gaacgggatt tcaacaggca caagcaacca
    11581 tgacttcttc tcacaagtcg gaaacggcta tttgttaggt catgtagaag cgatgaaaga
    11641 cgacgattat ttgattggat tggaataagg atttatttta attcaaaaat ttttcgaaaa
    11701 ataaaaagag tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat
    11761 tcacttgttt cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt
    11821 ctcaataaat agattttccg gatatttaat attatcgtct taatcactgc atagacaggt
    11881 atgcaaatca gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg
    11941 atcgtcaaag gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca
    12001 tctaattgtt ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata
    12061 aacaaaccga caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata
    12121 ttcgtgattc cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt
    12181 gccaaaaagc tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca
    12241 gataatgttt cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga
    12301 aagaagtgag acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata
    12361 aatggaacgg taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca
    12421 cttgcagata aactgactaa tatgatattg aacaaattac taagagataa atttgctgat
    12481 gctaattgtt cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac
    12541 catttgcttg tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc
    12601 attaactgag gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac
    12661 gtcaataaaa aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga
    12721 tcgaacatat aatagataaa accagcaaca agcaatggta aaaaaattgc cgaaagcatt
    12781 ttcaatattg gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg
    12841 agtactagct ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat
    12901 ctcctccaag gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga
    12961 ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact
    13021 taaacaaaaa cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat
    13081 aaacggtgtt caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga
    13141 actattttta tttggctggg cttattactt gatgtaggac atctatttta caacctcaga
    13201 tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact
    13261 cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt
    13321 acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt
    13381 tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt
    13441 ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa
    13501 tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc
    13561 cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga
    13621 tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt
    13681 catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac
    13741 tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg
    13801 tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata
    13861 gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct
    13921 aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt
    13981 tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa
    14041 cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat
    14101 ttttcctttg tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct
    14161 actattgaaa attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg
    14221 tgattttgca atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg
    14281 attgcatcag ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt
    14341 gaaccgattc ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt
    14401 ttgtttgcat tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa
    14461 gcaccaggtt cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg
    14521 attggatgtg catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct
    14581 tttccatctg gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg
    14641 aaatgacggt agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca
    14701 gaagtcattt cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt
    14761 gtgcttcctg ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt
    14821 tcaaatgctt tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt
    14881 gctcctttat attttggtat agcccaatta tagcattctt tttttctttt tacaataatt
    14941 agtatagacc aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg
    15001 tttcataaga tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac
    15061 tgtggacgct ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac
    15121 attaaacaac attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca
    15181 attaaatgct ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt
    15241 gattaaaatc atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa
    15301 tattcacaaa ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga
    15361 atttctaaaa actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta
    15421 ccaaacatat agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac
    15481 ggaattagac caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg
    15541 gttggggatc gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg
    15601 ttcaaaggtg attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga
    15661 accaaccaat cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt
    15721 tgacggtgcg tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca
    15781 tatcgcagac atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt
    15841 gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca
    15901 aatcgaaaag accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc
    15961 aaaaagccga caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag
    16021 taaagctgtc tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac
    16081 tgatttagtc atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt
    16141 tggtgaaaat attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac
    16201 actgatcggt gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat
    16261 caattacttt tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt
    16321 aagcagtcaa tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg
    16381 gttagttaat caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa
    16441 ggtgaaactt gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac
    16501 gaaccatatc gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg
    16561 aacagtaatc atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat
    16621 tgagattgaa ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa
    16681 gttttgtcac aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta
    16741 gctcaaaaaa cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg
    16801 cagaacagct tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct
    16861 ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt
    16921 acacctaacc agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct
    16981 ccaataaagt acataaacat aaataca
//
LOCUS       DAZUMM010000059        16482 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000059 DAZUMM010000000
VERSION     DAZUMM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.059"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            204..1478
                     /locus_tag="ACG8E6_001908"
     CDS             204..1478
                     /locus_tag="ACG8E6_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5062315.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            1532..2959
                     /locus_tag="ACG8E6_001909"
     CDS             1532..2959
                     /locus_tag="ACG8E6_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFS5062316.1"
                     /translation="MTKKAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVG
                     SGVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            2972..3844
                     /locus_tag="ACG8E6_001910"
     CDS             2972..3844
                     /locus_tag="ACG8E6_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5062317.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            3939..4934
                     /locus_tag="ACG8E6_001911"
     CDS             3939..4934
                     /locus_tag="ACG8E6_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5062318.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKNN"
     gene            5617..6232
                     /locus_tag="ACG8E6_001912"
                     /pseudo
     CDS             5617..6232
                     /locus_tag="ACG8E6_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(6372..7412)
                     /locus_tag="ACG8E6_001913"
     CDS             complement(6372..7412)
                     /locus_tag="ACG8E6_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5062319.1"
                     /translation="MASIVKRGKSYRAQVSLYKNGQHKKLSKTFATKKEATLWALEME
                     LAKGEGKELAERTTTFADFFENWIYLVKVNDIKETTFQNYVRTLGVIRNLFEDIQLKD
                     LNDIVVQKKIDEYAETHSRKTTHEVLLKIKTALRDAYARGYIANDFARLVKTRGVNPP
                     KRNKALSITNFKKLRNYVLQHPEDEFNVLVLLALETGMRRGELLAIKPENLYEYGIEV
                     RHSISPTSDDTSLKTQNAKRDVSINKEVYELIRQIPVKENGYIFSFGGFKQSEQLAEL
                     LESLGIEKTTFHGLRDTHASFLFAQDIDIAYVSKRLGHINIQTTQNYYLELMPEKKHQ
                     QDADALNLLSSL"
     gene            complement(7507..7704)
                     /locus_tag="ACG8E6_001914"
     CDS             complement(7507..7704)
                     /locus_tag="ACG8E6_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5062320.1"
                     /translation="MSINKNTKDYPYLLTREQASRFLGIDPKSFDKYIRPHNDLERFM
                     VGKHERYTMKSLIDFIESQSV"
     gene            complement(7748..7951)
                     /locus_tag="ACG8E6_001915"
     CDS             complement(7748..7951)
                     /locus_tag="ACG8E6_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062321.1"
                     /translation="MSNKKAMENINHIIEMIDFFQSGNGVINKKDNDSDYLRHIYTPE
                     DILGNLQHSAKEALKQLKNGELK"
     gene            complement(7961..8440)
                     /locus_tag="ACG8E6_001916"
     CDS             complement(7961..8440)
                     /locus_tag="ACG8E6_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062322.1"
                     /translation="MRHIAKEVDDWTRVEAEFSGDYAHQLTDAIKECSTDEQLKNLII
                     SSLLDRYMFFYVNSNRPHKITRLMLELLDEKNFHFESPSPRNNLLEQSIDHLIKGSGL
                     LPTLWKVQQIWGGDTAKELIDYLYKQYYEEFEPNDDHIAWVNKYKNIYLLDGKPWKE"
     gene            complement(8446..8640)
                     /locus_tag="ACG8E6_001917"
     CDS             complement(8446..8640)
                     /locus_tag="ACG8E6_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062323.1"
                     /translation="MASTNKSYLQKVIDDNKEKPYKRNNSKVTDVSKDFQAQTLYIGA
                     RSRNTKVHLRIYDKKEEQLS"
     gene            complement(8627..9118)
                     /locus_tag="ACG8E6_001918"
     CDS             complement(8627..9118)
                     /locus_tag="ACG8E6_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062324.1"
                     /translation="MIKPDIDQFTLVLQTTGVFDFDEWRDWVAKSLISTFLIKSKMHK
                     LFDNFGEADVKLPEGYTIGYSFINAPFYFCIAYHEAFSKMGVIVKYSAYAWHEYRKRY
                     EAEFNEPIHLHTFLKMIDSDEYSFRLSRIDICYDFINEGIDIAKLKRSIEEKRTELRY
                     GKY"
     gene            complement(9347..10648)
                     /locus_tag="ACG8E6_001919"
     CDS             complement(9347..10648)
                     /locus_tag="ACG8E6_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS5062325.1"
                     /translation="MKKTYRMQRGFISFLFIGFFLILWFFFSKLYPLIVEQITIIQLP
                     HIESNIFLFPVGLVVVGLFVSWSIRNKLWFGLFFSVHYYLMIKRIRRHVKDARFEDER
                     EFDNRLVKLPIIKIEFDDNRARKSGKVFIQNSIKFDKKLEDMRIDAALEGYVSERQYL
                     SKDRNWYIYEFYDVDSQKQLEIKSASDLIEWSNKKTDDYSLRLDERATVPFHHMGLVG
                     QTGSGKSFFIQMLVEQVLSKKVSHELFVIDPKRTDVYQMAKRTVGDERTADKTNAIEL
                     INQFHERMKKRQDELQDYFISNRNKTYRDAGLPALILLIDEFGALRESWKTLAKKERD
                     EVDSILSDVAFMGRQLGCILWVATQQMNAQTMPTAIREQLVLKIALGDSDEQTYRTLF
                     SSGVDIPPVQFTAGQGIYSYPGLASVDKPRMLAVPYCSYLE"
     gene            complement(10760..11107)
                     /locus_tag="ACG8E6_001920"
     CDS             complement(10760..11107)
                     /locus_tag="ACG8E6_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062326.1"
                     /translation="MKKLRQFNKFDAEVFFKDKDVRVMAHEPWNEYEDGQVTKELGTK
                     YKCVIATDNTKYEGNDVQPDLNAGEQIDVKVTQPPKSYKKFSRITFVNPTASVYGTFM
                     NELSVKADDVDIV"
     gene            11283..11870
                     /locus_tag="ACG8E6_001921"
     CDS             11283..11870
                     /locus_tag="ACG8E6_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5062327.1"
                     /translation="MLNDKADDMLNEEVAQNEILDFIDMKYGANQLVTQRTIQRWLEE
                     LGIDCIQPRPKRNRDIRYRKSDVLKLEEIKRTNLLRKKNKELARKDIEKIATDMQKKE
                     IEYNQWLASMTDVDRRQQDIDDMYDYKVPALNKIREDKLEMCFRKLFPDVKFDIEYLE
                     KILFTINEPSMHSDLEYGQAIVDLKEKAYIKNYKD"
     gene            12284..12727
                     /locus_tag="ACG8E6_001922"
     CDS             12284..12727
                     /locus_tag="ACG8E6_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003726066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFS5062328.1"
                     /translation="METILEIVRIEQVICEAEEGVNYTIKGPKDGAEIASRFIGRDDR
                     EVFFVMCLNTKNHVVAVHRCHVGSLNASIVHPREVFKSAILNNAASIIVAHQHPSGDI
                     TPSMEDINVTKRLVEAGKLLGIEVFDHLVVNGDSKFTSLKEKGHF"
     gene            12855..13601
                     /locus_tag="ACG8E6_001923"
     CDS             12855..13601
                     /locus_tag="ACG8E6_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010721123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFS5062329.1"
                     /translation="MTEEIIKCKTRVQKHGEVFTPKRIVNQMLDIPEIKEACENLTTT
                     FLEPSAGEGAFLVEILERKLKMVSEKYNKDLVQFENYSLLALTTLYGIELLEDNAQTC
                     VMNMFQKYYDYYKEQIQYHKGKMNQKVLDSAKRIISLNIGNGNFLTRKAVDGTPLIFS
                     EWEAINLRKNTKNIKVQRTEYTFDEIYENTQKENGKALRQPKTEPEQIDIFSFLDEPI
                     DKKEEVVKEMQYIPVKIIDVYKEEMEEANG"
     gene            13594..>16482
                     /locus_tag="ACG8E6_001924"
     CDS             13594..>16482
                     /locus_tag="ACG8E6_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS5062330.1"
                     /translation="MDKTVIKPFQEIKYKVYGYTLPEVPNHDGYVKIGDTTRDAVTRI
                     FEQVGTAGLNPKILFEKLARKSDGKWFRDKDLHKFLILNGIEKKNFNSRADEWFYFNG
                     TLEKAEELTNKYINRDYDEIQIDDKRSDYVLRNEQQQAVEKTYEYYQSKQEPKEFLWN
                     AKPRFGKTLTTFDFIRNLNATNVLIVTNRPAIANSWFDDFHKFIAWQEPQMKFVSETD
                     ALNGKAMTRDQYLEFIYTTSHVNPTQIAFISLQDLKGAEFAGGMYKKLEWVAGLNWDL
                     LVIDEAHEGIDTRKTDIAFSQLKREFTLHLSGTPFKALAKGDFSEEQIFNWSYVDEQE
                     AKENWDPTIGSNPYESLPTLNLFTYQMSKMIEDEVSAGLTIEEEKNVDYAFDLNEFFR
                     VKENGRFEYEESVVRFLDNLSSGKFPFSENQYRNELNHTFWLLPRVNAAKALEKLLNA
                     HPTFKDYKVVLAAGDGVSLHDGAEAEALDLKENTKSFDKVRNAIEQNDKTITLSVGQL
                     TTGVTIPEWTAVFMLNNIESPSLYFQAAFRSQNPYEYEKDGKLFRKENAYIFDFSPDR
                     TLRLYDEFANNLSSGSSKTSEERKRKIKKLLNFFPVIAEDEEGTMHEIDASEVLTIPT
                     RITSREVVKRGFMSNLLFANISGIFSGDSPFKEILDKIPPEKNKRLEKPREVSITDPH
                     LNDEGEVEIPKEVVLGKSKELFGEKIYSSIEVVDEVVSSFEEAEEKQKESIKKLAKDI
                     TASFSDGFNNLKESFKLNKGQTEKLKESVNNSIEDIITEKSLTLSNQVKEIEEEYTKK
                     KQEAEKKNDETELKAAIQQYEEEKQKIEESFADDINKEVENTINTAVEEQIEKVEEKK
                     KKTTEDDVRDHLRGFARTIPAFLMAYGDNQTRLENFEENIDEATFEDITSITIEEFKK
                     LRDGFEYEDDNGNRKVIHGLFNEVVFNASIREFLDTKKRLANYFDESLE"
BASE COUNT         5699 a         2524 c         3070 g         5189 t
ORIGIN      
        1 taaaaaatag tgccattaaa aaagaaaagc gacacaccaa taaaagtcat atcctgtgac
       61 attatatggc attatccaaa tagaataacc ggttgacatg taagcggatt tcttttataa
      121 ttttaggagt acaagtcctt ttctaaaaat gttaaccggt tatcatgaaa ggggatacaa
      181 atatatcgtt ggaggataaa aatatgtcaa aagttaacgt atttaaaaat caatcttatt
      241 tacaaagttc agctacatta ttactatttt ttgcttcttg gggtgtttgg tggtcatttt
      301 ttcaactttg gctaacatct gaatcaaatg gtttagggtt atctggcagt gctgtaggaa
      361 cagtattctc ggcaaattcg ttagttacct taattttgat gtttatttat ggaacattac
      421 aagataaatt gtatattaaa cgaaatttat taatttttgc ttctgtatta gcgacacttg
      481 ttggaccatt ttttatatgg atatatgggc cattgctaga taacaatttt aatttaggca
      541 ttattatggg agcgctattt ttgtcagctg gatatttagc ttctgtagga gtttttgaag
      601 ctgtgtcaga aaggtttagt cgtttatttg gctttgaata tggacaagca agggcgtggg
      661 gatcatttgg ttatgccttg gtagcgcttt tggcaggatt tttatttgta aaaaatcctc
      721 atttaaactt ttgggcggga tctttctttg gttctttact attgttaaat ttattatttt
      781 ggaaccctaa agttgaacgg gaagcaaatc aaaattttaa tcaagaacaa gctgaatcaa
      841 atagtattcc ttctttaaaa gaaatgtttg atctaatgaa actgcctcaa ttatggacga
      901 taatcatctt tattgttttt acatggacat tttatacggt attcgatcaa caaatgtttc
      961 cgggatttta tactggtttg ttttcaacat cagctaatgg tgaaaaaata tatgggacat
     1021 tgaatgctat tcaagtattt tgtgaagcgt taatgatggg aattgttcca atcattatga
     1081 gaaaattagg ggttcgaaat actttgttat taggtgtaac cattatgtgt gtacgaattg
     1141 gattgtgcgg gtttgcctcg acaccattat ctgtttcatg cataaaaatg ttgcatgctt
     1201 tagaagtacc attatttaca ttaccaatgt ttcgctattt tacacttcat tttgatacaa
     1261 agctatcagc aaccctctat atgataggat ttcagatagc tgctcaaatt gggcaagtga
     1321 ttttatcaac accattggga atattaagag acaacgttgg ctatcaacca acatttaaaa
     1381 ttatttctct tattgtatta ctagcaggca tatatgcatt ctttattctt aaacaagatg
     1441 atagagatgt tcaaggggat ccatttattc gaggataatg gaaaccaaca gaataaaaaa
     1501 caattttatc tagaaagtga gcgaatatat tatgacaaaa aaagctatag aattaacaaa
     1561 tacacgttat cgtttgggat atcatgtgtc tgcaccatca ggatggatta atgatccaaa
     1621 tggattttgc tattttgatg ggtattatca tgtgttttat caacatcatc catatagtgc
     1681 tgaatgggga ccaatgcact gggcacatgc tagaagtaag gatttagttc attgggaatc
     1741 tttaccactt gctttgactc ctggagatca agaagatgaa ggtggttgtt tttcaggaag
     1801 cgctattgaa aaaaatggtg tgttgtatct gttctataca ggacatcact attatggaga
     1861 caatgatcca gatcattttt ggcaaaatca aaacatggcg tatagtacgg atggcattca
     1921 ctttactaaa tatgaaaaaa atcctgtaat agcaaaagca ccagaagata acacacatca
     1981 tttcagagat ccaaaagtat ggaaacacga tgaaaaatat tacatggttt tgggtagtca
     2041 agaaaaagat ggattaggtc gtgctattgt ttatcagtcg ttagatttat taaattggga
     2101 ttacattgga ccaatttcta aagcgaagga agtacttaca gaaggattta tgtgggaatg
     2161 cccagacttt tttgaattaa atggaaaatc tattctttta ttatcccctc agggaattga
     2221 agcaaatgga aaagattatt taaatctata tgaaacaggc tactttatcg gtgattatga
     2281 ttatgagaca gcaaaattca ttagaggaaa tttttatgaa ttagataagg gacacgattt
     2341 ctatgcaaca caaacaactt tatctccaga tggtagacgt attgttatgg cctggatgga
     2401 tatgtgggaa agccctatgc ctgaaaaagt agacggatgg gcaggagcat taacgcttcc
     2461 tcgagaatta gagttaattg gagatcgttt gtatatgaaa cctgtgagag aattagaaca
     2521 acttcgagtt ggcagcggcg ttgaaacgac agttaatttg gcaggagaaa taataatcgc
     2581 tcaagatgct tcattcaaag aagctctaat tgatgtacta ttgactaaat caaaaaatca
     2641 agaaatcaaa ttttcattaa aaacaaaaga tgaagagtta atcaatattt tttattctaa
     2701 ggataaaaat gaatgtacct taactcgtaa cggcaaaaat gatccaaggt atggaaccat
     2761 tcagatgtgt gatagattat cacttcgagt gtttatcgat aaaagttcgg cagagatttt
     2821 tattaatgat ggcgagcttg tttttacaga gcgttattat acagaaaatc aaccaagtat
     2881 gcatttgagt gttagtgaag aagcttttgt ctcgagtaca atttatcatc taaaaaaaga
     2941 tgcaatttct tacgagtaag gaggttttat gatgttaatt ggtagcatag aagcaggtgg
     3001 gactaaattt gtatgtgccg tgggagacga ggattaccga gtacaagacc aaataatttg
     3061 tccaacgacc acccccaggg aaacattgaa aaaaactgtt gactatttta aacagtttga
     3121 tataaatgca ttaggtattg catcatttgg tccaatagaa attcgaaaaa attcaccaaa
     3181 atcaggatat attacgtcta caccaaaggt gggatggcga aatgttaatg tgttaggtta
     3241 tttacaagat gagctgaatg ttccaatgtc ttggacaacg gatgtgaacg gatcagctta
     3301 tggagagtac atcatgtcca ctttatcgaa tgaaaaaata aattcattag tttattacac
     3361 cgttggtact ggcgttggtg gtggagctgt tgttgacgga aaactattag gatcgtttgg
     3421 tcattcagaa atgggccata cgtttgtaaa acgccatcct gatgatattg attttaaagg
     3481 aatttgtcct tatcatggtg attgtttaga aggcttagtt gcgggaccaa cgtttgaagc
     3541 acgttttgga aaaaagggtg aagacgtttc tttaacggat cctatttggg atgtaatggc
     3601 ctattatcta gcacaagctg tgattcaaac aacattgatt atacgaccgg ataaaatagt
     3661 atttggtgga agcgtagtaa atgaaaaatt gttagaaaaa attcgtgtta atgttaataa
     3721 attattggat gattacgtgg aaataccacc gttaagtaaa tatattacgc gtcctttaat
     3781 taaaaataat ggatcggcaa cactaggaaa ctttgctttg ggcttgaatc aattacaaga
     3841 ataaaacata gaaaaaatat cgattatcag gtatagtagt tctatagagt tctttttttc
     3901 gatagttaat tattgcatta tttaggagag acaagagaat gcgcccaaaa ttagaagatg
     3961 ttgccaaaag agcaaatgta tcaaaaacaa ctgtttcacg tgtattaaac aatcgtggct
     4021 atttaagtca aaaaacgatt gataatgttt ataaggctat agaagaactc aattatcaac
     4081 cgaacgttgt agcgcgtcaa ttatttcaaa agaaaactaa cattgtcggg ttactatttc
     4141 ctacagtagc caatcctttt tttagtgaac tcgttgaggc attggaaaaa aagttatatg
     4201 aaattggtta taaggtatta ataggcaatt cgatgaataa taaagaaaag gaaacaaatt
     4261 atttaaatca gttattgtct gatcaagtag atggtttaat tgttggtaca cataaccaag
     4321 gaattcagga atataaatat cagaatttac cgattgttgc aattgatcga gtgatgaatg
     4381 aagatattcc agttgttgaa tccgataact acaatggtgg aaaacttgca accaagttat
     4441 taattgcaca aggtgcaaaa aatattattc atacaaacgg tccaattgac ttacagactc
     4501 cggctaatcg tcggagacta gcctatgaag atactatgaa agcatatcag ttaattccac
     4561 gaactgtgac attagatttc aatatttctt atgtaaagaa aaaacagatt tttttccaaa
     4621 tgtttgagga ttatcctaaa atagatggta tttttgcttc aaatgacatc gatgcggccc
     4681 taatattaca agtagcaaaa gaaaaggggt taaatgtacc agctgattta ttagtagtcg
     4741 gatatgatgg tactttaatg actcggagta tattaccaga tctgacaaca gtaatacaac
     4801 caattaatga tatagcagac acagctgttg ctattttgat gaaacgaata aataaagaag
     4861 aaacaaaaaa ggaatatatt ttacctgtca cattatggat ggggaagaca tcagtaaaga
     4921 aaaagaacaa ttaaatttat aaagactatc aatgataatt tcattattaa ttcgtgatat
     4981 aaaaaattgt taaatctctt ataatagact atgatacaaa atcatgcttt taatctgtgt
     5041 agatgttgcc aaatatttga aagacataga acttgtcaga aaaaccttaa caattcctca
     5101 atgggcggat aagctaggca aaagggctgg cataaatttt tcagtccttc taacagaatc
     5161 aattgcagat aaagcagata acattcttcg ttctggaaga aataattaga actcaaaagg
     5221 gtcagaaact taaaagtttt tgatcccttt tttattcctc atttcatagc ttttttagta
     5281 gctcaattaa ttaggtcaga aagttacttt tttaaaagga caccagagta taccctgttg
     5341 ggctcttggt gttctattga atgtaaatga gtgtcatttt tatatttaat tggcacagtt
     5401 ttttctaatt atttgtcata ttcatagtca actatagcta caaaagcaac taattttgga
     5461 gatagattga gatatttaaa aatctatcat gaaaaattag ttgcttttgt cacttagcgc
     5521 tgaaaattta tgccatttat ctagaaaaat gacaacagag ttgggcaaca gaatttaaaa
     5581 atgatccgga tttcagaatt ttaaaggagt tttattatgg cgaaaattgg gtatgctcga
     5641 gtaagcagct tagatcaaaa tttagaccga caattagaat tattatcaga gtgtgataaa
     5701 atttttagtg ataaagcaag tgggaaagat accaatcgag aaggatttca agaaatgatg
     5761 aaattcatac gtgaaggtga tattgtcgtt gtgacagaat taaaacgact tggacgaaat
     5821 aataaagagc taacagaaac gatgaattta attcagctga agaaagctac actggaagtt
     5881 ttgaatttgc cagcgttaac aggtatcact gatgataatt tacgtcgatt acttaataac
     5941 ttaattatcg aactatacaa gtaccaggcc gaagaagaac gtcggtatat tcgcgaagca
     6001 acaacaagga attgcactcg ctaaagcaaa agggaaatac aagggaggta cgctaaaata
     6061 tcgtgagaat gatccgcgac ttcaattagc atttaatttg tttttgactg gatctacaga
     6121 taaagaagtt gaagagctaa cagggattaa tcgtagaacg tttagaagat atcgaatgaa
     6181 acatggtatt actaatgaag ttagggagca aaaaaataaa tctttcagtt gaggctaata
     6241 taattttgca agaaaccagg tcttaagaaa gctcgtaaag cttcacaatt ctcaaaacgt
     6301 taataggttt gcctatatac caacgtttac aaaggttttt tggttcaagt tggttcaaat
     6361 cagcttgaac ctcataaaga acttaacaga tttaaagcat cggcatcttg ctggtgcttt
     6421 ttttctggca ttaattctag gtagtaattt tgggttgttt ggatgttaat atgccctaac
     6481 cttttcgata cataagcaat atcaatatct tgtgcaaaca agaatgatgc atgtgtgtca
     6541 cgtaaaccat ggaaggttgt tttttcaata cctaaactct ccaatagctc cgctaactgt
     6601 tcggactgtt taaatcctcc aaaactaaaa atatacccat tttcttttac cggaatttga
     6661 cgaattaact catatacttc tttgttaatt gacacatcac gctttgcgtt ttgtgttttt
     6721 aaagacgtat catcagaagt aggactgatt gaatgcctaa cttcgatacc gtactcgtaa
     6781 aggttctctg gtttaattgc aagtagttct ccacgtcgca tacctgtttc aagtgcaagt
     6841 aatacaagta cattaaattc atcttctgga tgctgtaaga cgtaattacg aagcttcttg
     6901 aagtttgtaa tggataatgc cttgttgcgt tttggcggat tgacaccacg agtttttaca
     6961 agtcgtgcaa agtcatttgc aatatatccc cgagcatatg catcacgtaa agcggtttta
     7021 atttttaaca atacttcatg tgttgtttta cgagaatgag tttccgcata ttcgtcaatt
     7081 ttcttttgaa caacaatatc gtttaaatct tttaactgaa tatcttcgaa caagttgcga
     7141 atgactccca atgttcgaac gtaattctga aaagtcgttt cttttatatc attcactttt
     7201 acaagataga tccagttttc aaagaagtca gcaaatgtcg ttgttcgttc tgcaagttct
     7261 ttcccctcac cttttgctaa ttccatttct aaagcccata aagtagcttc ttttttggtt
     7321 gcaaatgttt tagaaagctt tttatgctgt ccgttcttat agagtgacac ttgggctcta
     7381 tatgacttcc cgcgttttac aatactagcc atactatcaa ttcctttcaa aaaataagtt
     7441 gatagatgct agttgaatac acctgtattg tattctgtaa ctagcataat atcaactgtt
     7501 aaaacattag acagattgtg attcgataaa gtcaattaaa gatttcattg tatatcgttc
     7561 gtgttttcct accataaaac gttcaagatc attatgtgga cggatatatt tgtcgaatga
     7621 ctttgggtct atgccaagaa aacgagatgc ttgttcacga gttaaaaggt aaggatagtc
     7681 tttagtgttt ttattgatgc tcatgtagca ccatctcctt tgtaataaaa taatatccaa
     7741 gtaataatca cttcagttct ccatttttta actgttttaa tgcctcttta gcagagtgtt
     7801 ggagattacc tagaatgtct tcaggtgtat agatgtgacg caagtagtca gaatcattat
     7861 cttttttatt aatcacacca ttaccacttt gaaaaaaatc tatcatttca ataatgtgat
     7921 taatattttc cattgctttt ttattgctca tatcgtaatt tcactccttc caaggtttac
     7981 catcaagtaa atatatattt ttatatttgt tcacccatgc tatgtgatca tcattgggtt
     8041 cgaattcttc atagtactgt ttatataaat aatcaattaa ttcttttgct gtgtcgcctc
     8101 cccatatttg ttgaactttc cacaatgtgg ggagtagacc agacccctta attaaatggt
     8161 ctatcgactg ctctaataag ttatttcgtg gagatggtga ttcaaagtga aagttctttt
     8221 catctaacaa ttcaagcatt aatctagtta tcttatgagg tcgattagaa ttaacataaa
     8281 agaacatgta gcgatcaagt agagaactaa taatcaaatt tttaagctgt tcatctgtac
     8341 tacattcttt aattgcatct gttaattgat gggcgtaatc acctgaaaac tctgcttcaa
     8401 cacgtgtcca gtcatccact tctttagcga tatggcgcat gtgtttcatg acagttgttc
     8461 ctctttttta tcataaatgc gtaaatgcac ttttgtattt ctgcttcttg ccccgatata
     8521 caatgtttgt gcttgaaagt ctttggatac gtcggtcact ttcgagttgt ttcttttgta
     8581 tggtttttct ttgttgtcat caataacttt ttgtagataa cttttattag tacttgccat
     8641 atcgcaattc agtccttttt tcttcaattg agcgttttaa tttagcaata tcaatacctt
     8701 cattaataaa gtcataacag atatcaattc gtgaaagtct aaaactatat tcatccgaat
     8761 caatcatttt taaaaaggtg tgtaaatgaa taggttcatt aaattctgct tcatatcgct
     8821 ttcgatactc atgccatgca tatgcactat atttgacaat cacacccatt ttcgaaaatg
     8881 cttcatgata agcgatacag aaataaaaag gtgcattgat gaatgagtaa cctattgtgt
     8941 agccttctgg tagcttaacg tcagcttcac cgaagttgtc aaatagctta tgcatcttag
     9001 atttaatcaa gaaagtactt attaggcttt ttgctaccca gtcacgccat tcatcaaaat
     9061 caaacacgcc tgttgtttgt aatacgagtg taaattggtc aatatctggc ttaatcatgt
     9121 tctattcgct ccttttccta agaaaacttt aggtaaagtt tacttggtgt aaaagcctat
     9181 aagaataggc tttagtctgg gttttactcc tgactggagc tggggtgatt accaatcccc
     9241 cagctctcct aaaggaggga agcacggagc ggaacctttc cctgcctaac ggcgggtccc
     9301 tttccgctca ttgtgcaacc gttccttaag aaaaagctaa gtgctattat tcaagatagc
     9361 tacagtatgg gacagcaagc attctcggtt tatcaacact tgctagccct ggataactgt
     9421 aaatcccttg accagcagta aattgaactg gaggtatatc cacaccagaa gaaaacagag
     9481 ttctataggt ttgctcatca ctatcaccta aagcaatctt caaaacgagc tgttctctaa
     9541 tcgctgttgg catcgtctgt gcgttcattt gttgcgtagc gacccacaga atacacccta
     9601 gttgacgacc catgaaagca acatcagata gtatgctatc gacttcatca cgttcttttt
     9661 tcgctaaagt cttccatgat tctcttaatg ccccgaattc atctataagc aaaattaaag
     9721 ccggcaaacc agcatcccta tacgttttgt tacgatttga tatgaaatag tcttgtaatt
     9781 catcttgtcg ttttttcata cgctcatgaa attgattaat caattcaatc gcatttgttt
     9841 tatcggctgt tcgctcatca ccaacggtac gtttagccat ttggtaaacg tcagtacgct
     9901 ttggatcaat tacgaataac tcatgactga ccttttttga taatacttgt tcaactagca
     9961 tttgaataaa aaacgactta ccactacctg tttgcccaac taatcccata tgatgaaacg
    10021 gtacagttgc acgctcatct aaacgtaggg agtaatcgtc agttttcttg tttgaccact
    10081 caatcaagtc acttgctgat ttaatctcaa gttgtttttg tgaatctaca tcatagaact
    10141 catagatgta ccaattacgg tctttagaca aatattgacg ttcacttacg tatccttcta
    10201 acgctgcatc aattctcatg tcttctaact tcttgtcgaa tttaattgag ttttgaataa
    10261 acacttttcc tgatttcctt gcgcgattat catcaaattc aattttgatt atcggtaatt
    10321 taactaaacg attatcaaac tcacgttcat cctcgaatct agcatcttta acatgtcttc
    10381 taattctttt tatcatcaaa taataatgca cagaaaaaaa tagaccgaac cataatttat
    10441 tgcgaattga ccaactaacg aacaaaccaa caaccaccaa gcctacagga aacaagaaga
    10501 tatttgactc aatatgtggg agctgtatga tcgttatttg ttcaacaatc aatgggtaca
    10561 gtttagaaaa gaaaaaccat aaaataagaa aaaatccaat gaataaaaat gaaataaaac
    10621 cacgttgcat ccgatacgtt ttcttcacat attccacgtc ctaatattgt ggttccgccc
    10681 ctgataaggg gcggtcacag gtataaaagt gtatttgacg atagaaaggg aactgactac
    10741 cgttcgttct ttacttcggt taaacaatgt ctacatcatc tgctttgaca gaaagttcat
    10801 tcatgaacgt tccatacaca gaagctgtcg gattcacaaa cgtgattcta ctaaatttct
    10861 tataactctt tggaggttgt gtcactttca catcaatttg ctcaccagca tttaaatctg
    10921 gctgaacatc attgccttca tattttgtgt tatccgtcgc aataacgcac ttgtacttag
    10981 tacctaactc ttttgtgact tggccgtcct catactcgtt ccacggttca tgagccatca
    11041 ctcgaacgtc cttatccttg aaaaaaacct cggcatcgaa cttattaaac tgtcttaatt
    11101 tcttcatagt gttttgcact cctttatttt tgatctacct atattttagc taatacttgc
    11161 cattttatca agacatgtca tcatgacaaa taacaaaaat tgttgcactt atagtatgtt
    11221 aatggtatta taatttataa tgacattcga taatttataa cgacatttat gggggtgtta
    11281 aaatgttaaa tgataaagca gatgatatgt taaatgaaga ggttgcacaa aatgaaatat
    11341 tagattttat agatatgaaa tatggggcaa accaactcgt gactcaaagg acaatacaaa
    11401 gatggttaga agagttaggc attgactgca tacaacctcg tccaaagaga aatagggata
    11461 taaggtatag gaaatctgat gtcttaaagc tagaagaaat aaagagaact aatttattga
    11521 ggaaaaagaa caaggaatta gcacgtaaag atatagaaaa aatagcaacc gatatgcaga
    11581 aaaaagaaat agagtataac caatggttag ctagcatgac agatgttgat agaagacagc
    11641 aagatattga tgacatgtat gattataaag tacctgcatt aaataaaatt agagaagaca
    11701 aattggaaat gtgttttaga aagttgtttc ccgatgttaa gtttgatata gagtacttag
    11761 aaaagatttt gtttaccata aatgaaccat ccatgcattc agatttagaa tacggacaag
    11821 caatagttga cttaaaggaa aaggcttata taaaaaatta taaagattga aagctcatag
    11881 aagaaaggga gcaaacccgt taatcgttat cacaataaag taggataaaa tttatttgaa
    11941 tttgtaatag ggcaagtgca gatagggagt aggtacatcc gtcagatttt tccttttcag
    12001 ctcccctaat tgtaatctcc taatgcgtcc gtaaagaatg gacgcattat cgcttatgta
    12061 aagggtcttc gttcttcgga tataatggca aagccaagtg gataagagtg cgtaataaag
    12121 caattaacgc tttaaacgca ctctaattta ttgggcttta ctcatcatat caacgaatct
    12181 cttgatagct gtcgctactt acgccacttg acattaatcg atgcggagat tgattgaatg
    12241 gtgaccaaaa aggtcaaaaa taacacgaga gaggttgatc tacatggaaa ccattttaga
    12301 gattgtacgt atcgaacaag ttatttgtga ggctgaggag ggtgttaatt acacgataaa
    12361 aggtccaaaa gatggagcgg aaattgcatc tcgttttatt ggacgtgatg atcgtgaagt
    12421 attctttgtt atgtgtttaa acactaagaa tcacgttgtc gcagttcatc gttgtcatgt
    12481 tggttcattg aatgctagta tagtacatcc gcgtgaggtg tttaaatcag cgatacttaa
    12541 caatgcagca agtatcattg ttgcgcatca gcatccaagt ggtgacataa caccatcaat
    12601 ggaagatatt aatgttacaa agagattagt tgaggcagga aagctattag gcattgaagt
    12661 gtttgatcat ttagtggtaa acggtgatag taaatttaca agtttaaaag agaaagggca
    12721 tttttaaaag ggaggaatct ccctttttct tcaagtaatt acggaactac cgaaaagtta
    12781 cgttcttaag ctgattatgg taaaatggaa gcagataata tgcgaaagct tacatataaa
    12841 ggggggggga gaatttgaca gaagaaatta ttaaatgtaa aactcgtgta caaaaacatg
    12901 gggaagtatt tactcctaaa agaatagtaa atcaaatgct agatatacca gaaataaaag
    12961 aagcctgtga aaatcttacg acaacatttt tagagccatc tgctggtgaa ggtgcgtttt
    13021 tagttgagat acttgagcgg aaattaaaaa tggtatcaga gaagtataat aaagatttag
    13081 ttcaatttga aaattactct ttactagcct taactacatt gtatggaatt gaattgttag
    13141 aagacaatgc ccaaacatgc gtgatgaata tgtttcaaaa gtattatgac tattataaag
    13201 agcaaattca atatcataag ggaaaaatga atcaaaaagt attagatagc gctaaaagaa
    13261 ttatctccct aaatattggc aatggtaact ttttaactcg taaggcagta gatggtacgc
    13321 cattgatctt cagtgagtgg gaagccatta atttaagaaa aaatactaaa aatataaaag
    13381 ttcaaagaac agaatataca tttgatgaaa tatatgaaaa tacacaaaag gaaaatggga
    13441 aagccctaag acaaccaaaa actgaaccag aacaaattga tatttttagc tttttagatg
    13501 aaccaattga taaaaaagaa gaagttgtaa aagaaatgca atacattcct gtgaaaataa
    13561 tcgatgttta taaagaggag atggaggaag ccaatggata agacagtaat taagcctttt
    13621 caggaaatta aatataaagt gtatggttat accttacctg aggttccaaa ccacgatggt
    13681 tatgtgaaga taggtgatac gacacgtgac gctgttacga gaatttttga acaagtaggt
    13741 acagctggtt taaatcctaa aatattgttt gaaaaactag ctcgtaaatc tgatggaaaa
    13801 tggtttcgtg ataaagattt acataaattt cttattctaa atggaataga gaaaaagaat
    13861 tttaatagtc gtgctgatga gtggttctat tttaatggaa cgctggaaaa ggctgaagag
    13921 ttaacgaata agtatataaa ccgtgattat gatgaaatac aaattgatga taaacgttca
    13981 gattatgtat taagaaatga acagcaacaa gcggtagaaa aaacgtatga atattatcaa
    14041 agtaagcagg agccaaaaga gtttttgtgg aatgcaaaac cgcgttttgg gaaaacatta
    14101 acaacatttg attttattag aaaccttaat gcaacgaatg ttttaatcgt aacaaataga
    14161 cctgcgattg ctaactcatg gtttgatgat ttccataaat ttattgcatg gcaagagcca
    14221 caaatgaagt tcgtatcgga aacggatgca ctaaatggaa aagcaatgac tagagatcag
    14281 tatcttgaat ttatttatac aacttcccat gttaatccca ctcaaattgc atttatttca
    14341 ttgcaggacc taaagggtgc agaatttgca ggaggtatgt ataagaaact agaatgggta
    14401 gctggactaa actgggactt gctcgtaatt gatgaagctc atgaaggtat cgatactaga
    14461 aaaactgata tagcatttag ccaattaaaa agggaattca ccctgcattt atcaggtact
    14521 ccgtttaaag ccttagctaa aggagatttt tctgaagaac aaatatttaa ttggtcgtat
    14581 gtagatgaac aagaagctaa agaaaactgg gatccaacaa ttggtagcaa tccttacgaa
    14641 agcttaccta ctttgaattt atttacgtat caaatgagta aaatgataga agatgaagta
    14701 tcagcaggat taacaataga ggaagagaaa aacgttgatt atgcattcga tttaaatgaa
    14761 ttttttagag tgaaagaaaa cggaagattt gaatatgaag agagtgttgt tcgcttttta
    14821 gataatctat cttcagggaa gtttccattt tctgaaaatc aatatcgtaa tgaactaaat
    14881 catacatttt ggctgttacc tcgtgtgaat gcagcaaaag cacttgaaaa attgttaaat
    14941 gctcatccta cttttaaaga ttataaagtc gtacttgcag caggagatgg tgttagtctg
    15001 catgatggag cagaagcaga agcgcttgat ttaaaagaaa atacaaaaag ctttgataaa
    15061 gttagaaatg caattgagca aaatgataag acgataacac tttctgtcgg gcaactgaca
    15121 actggtgtga cgattccaga atggacagct gtatttatgc taaataatat tgaatctcct
    15181 agtttatact ttcaagctgc attcaggtca caaaaccctt atgaatatga aaaagatgga
    15241 aaacttttta ggaaagaaaa tgcgtatatc tttgattttt caccagatcg aacattaagg
    15301 ctttatgatg aatttgctaa caacttgtct agtggtagtt ctaaaacaag tgaagaaaga
    15361 aagcgcaaaa taaagaagtt attaaatttc tttccagtaa ttgctgaaga cgaagaaggt
    15421 acaatgcacg aaatagatgc atctgaagta ctaacgatac ctacacgaat tacttcacga
    15481 gaagttgtta aaagaggctt tatgagtaat ttactatttg ctaatatatc aggtatcttt
    15541 tcaggagatt caccatttaa agagatttta gataaaatac cacctgagaa aaataaaaga
    15601 ttagaaaaac ctcgtgaggt ttctattact gatccgcatt taaatgatga aggggaagta
    15661 gagataccaa aagaagtagt gttaggtaaa tcaaaagaac ttttcggaga aaaaatttat
    15721 tcatcaattg aagtggttga tgaagttgta tcatccttcg aagaagctga agaaaaacaa
    15781 aaagaatcta ttaaaaaact tgcgaaagat ataacagcat cattttcaga tggatttaat
    15841 aacttaaagg aatccttcaa actgaacaaa ggacaaaccg aaaagctaaa agagtcagta
    15901 aacaattcaa ttgaggatat tataactgag aaaagtttaa cactaagcaa tcaagttaaa
    15961 gagatagagg aagaatatac aaagaaaaag caagaagcag aaaagaaaaa tgatgaaaca
    16021 gaattaaaag cagcgattca gcaatatgaa gaagaaaaac aaaagataga agagtctttt
    16081 gccgatgata ttaataaaga agtagaaaac acaatcaaca ctgctgtcga agaacaaata
    16141 gaaaaagtag aagaaaagaa aaagaaaaca acagaagatg atgttcgaga tcacttacga
    16201 ggatttgcac gtacaatacc tgcattccta atggcatacg gagataatca gactagatta
    16261 gaaaactttg aagagaatat tgatgaagct acctttgaag atattacaag tatcacaatt
    16321 gaagagttca agaagcttcg cgatggtttt gaatatgagg acgataatgg taatagaaaa
    16381 gtcattcatg gtttattcaa tgaggtagtt tttaatgcca gtatacgaga gtttttagat
    16441 actaaaaagc gtttagcgaa ttactttgat gaaagtttag aa
//
LOCUS       DAZUMM010000060        16381 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000060 DAZUMM010000000
VERSION     DAZUMM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.060"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..550)
                     /locus_tag="ACG8E6_001925"
     CDS             complement(152..550)
                     /locus_tag="ACG8E6_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFS5062331.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="ACG8E6_001926"
     CDS             complement(547..1320)
                     /locus_tag="ACG8E6_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS5062332.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="ACG8E6_001927"
     CDS             complement(1317..2000)
                     /locus_tag="ACG8E6_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS5062333.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="ACG8E6_001928"
     CDS             complement(2167..3054)
                     /locus_tag="ACG8E6_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5062334.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="ACG8E6_001929"
     CDS             complement(3051..3920)
                     /locus_tag="ACG8E6_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5062335.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="ACG8E6_001930"
     CDS             complement(4057..5343)
                     /locus_tag="ACG8E6_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5062336.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="ACG8E6_001931"
     CDS             5616..6194
                     /locus_tag="ACG8E6_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5062337.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="ACG8E6_001932"
     CDS             6194..7123
                     /locus_tag="ACG8E6_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5062338.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="ACG8E6_001933"
     CDS             7120..8856
                     /locus_tag="ACG8E6_001933"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5062339.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACG8E6_001934"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACG8E6_001934"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFS5062340.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="ACG8E6_001935"
     CDS             complement(9752..10261)
                     /locus_tag="ACG8E6_001935"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFS5062341.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACG8E6_001936"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACG8E6_001936"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFS5062342.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGREIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKASADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="ACG8E6_001937"
     CDS             complement(11562..12653)
                     /locus_tag="ACG8E6_001937"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFS5062343.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACG8E6_001938"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACG8E6_001938"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFS5062344.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACG8E6_001939"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACG8E6_001939"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFS5062345.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACG8E6_001940"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACG8E6_001940"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFS5062346.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4728 a         3621 c         2911 g         5121 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tacaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccgc tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgac gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctct accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 a
//
LOCUS       DAZUMM010000061        16048 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000061 DAZUMM010000000
VERSION     DAZUMM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.061"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(543..2048)
                     /locus_tag="ACG8E6_001941"
     CDS             complement(543..2048)
                     /locus_tag="ACG8E6_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5062347.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ACG8E6_001942"
     CDS             complement(2045..2731)
                     /locus_tag="ACG8E6_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5062348.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACG8E6_001943"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACG8E6_001943"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFS5062349.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="ACG8E6_001944"
     CDS             complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="ACG8E6_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS5062350.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4752..5321)
                     /locus_tag="ACG8E6_001945"
     CDS             complement(4752..5321)
                     /locus_tag="ACG8E6_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFS5062351.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5507..6409
                     /locus_tag="ACG8E6_001946"
     CDS             5507..6409
                     /locus_tag="ACG8E6_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS5062352.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="ACG8E6_001947"
     CDS             complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="ACG8E6_001947"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFS5062353.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="ACG8E6_001948"
     CDS             complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="ACG8E6_001948"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFS5062354.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="ACG8E6_001949"
     CDS             complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="ACG8E6_001949"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFS5062355.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLIKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLIENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKDGSQIPLENLSASLPLT
                     ANGYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            12973..13158
                     /locus_tag="ACG8E6_001950"
     CDS             12973..13158
                     /locus_tag="ACG8E6_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFS5062356.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(13220..14416)
                     /locus_tag="ACG8E6_001951"
     CDS             complement(13220..14416)
                     /locus_tag="ACG8E6_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS5062357.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNIFLSLFNFRK
                     VLRSRL"
     gene            complement(14586..16022)
                     /locus_tag="ACG8E6_001952"
     CDS             complement(14586..16022)
                     /locus_tag="ACG8E6_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS5062358.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNEVSVALWIVDILFIINFIILATI"
BASE COUNT         4663 a         3403 c         2766 g         5216 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attttttatt
     4441 taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt tttacatttt
     4501 ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata gtaaccacaa
     4561 tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc gtttaaccct
     4621 ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga tgttcttcta
     4681 gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat tagacacaat
     4741 atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag ttcagatagt
     4801 tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg ctggtattct
     4861 tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc ttcctcttct
     4921 tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga ctctgctaaa
     4981 ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc atctctactt
     5041 tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg cagttcaaca
     5101 ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta atgaacgata
     5161 tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac atcaagtatt
     5221 aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa agttaacggt
     5281 gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca ctcctaagca
     5341 acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt gacaccttgc
     5401 agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat catcctcaga
     5461 atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga acacgaaaga
     5521 tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg ctaattattt
     5581 ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag agtttgacac
     5641 cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt ctggaaagca
     5701 gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag ctgaaatcac
     5761 tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca atgaatactt
     5821 tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg agattaccga
     5881 tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt tagctgtatt
     5941 aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt tggacaaacg
     6001 tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg tctctttcaa
     6061 agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc ccgcagcatt
     6121 cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa gcaatgactt
     6181 gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg cagagattgc
     6241 tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc agcctcgctt
     6301 cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag aacaagtagt
     6361 ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa agaagagctg
     6421 tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta aacgatgccg
     6481 cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc gcctttagca
     6541 ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga agtcaatccg
     6601 ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc tttgtcagtt
     6661 gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc agcagatgaa
     6721 gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc taataattta
     6781 gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac tggtacacca
     6841 gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc ttttttagct
     6901 gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg gtaaacgccc
     6961 cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa gatatctgca
     7021 tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc agcaacgatt
     7081 gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt ttcagttaca
     7141 tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc attttcagct
     7201 tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc gtttgcagat
     7261 tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac gtcagaagct
     7321 tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt agcagtgata
     7381 actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat tggtactagt
     7441 tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc agaaactttg
     7501 atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa gatttgaaca
     7561 tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg tgctttacgt
     7621 acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat atcgtcagca
     7681 tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac ccctttacgt
     7741 gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc ttcgtctttt
     7801 tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc aacgtgaaca
     7861 tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac atgttcagga
     7921 tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc acgaccaaaa
     7981 tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt atctaaaaga
     8041 ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat acgtgataat
     8101 tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc agattcaatc
     8161 aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac gattttggtt
     8221 tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta cacagcttaa
     8281 atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat atagtgataa
     8341 gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa cgatttcgtt
     8401 gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga cagcgtaagc
     8461 accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac ggacaacgtg
     8521 tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct cagcgaattc
     8581 atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc cgcgatcgcg
     8641 gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg ggatgatgat
     8701 ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat tgcgtcccat
     8761 tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac ggtcaattga
     8821 ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc caggaatatc
     8881 attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca aagccattgc
     8941 gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga attttttcaa
     9001 ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac gtgcagaata
     9061 caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa gacgacgaat
     9121 atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca gtccttcgta
     9181 aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt ctccgccact
     9241 ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac tttatcctaa
     9301 gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct aacattattt
     9361 ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca agtagtattt
     9421 tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat aagttgcccc
     9481 tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt tcgttgcgtt
     9541 ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat cacttgtaat
     9601 tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca agtttgtgaa
     9661 ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag ttgcagttcc
     9721 tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc aacggcaaac
     9781 aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc tccactactg
     9841 tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc tctaacttcc
     9901 ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat cagctgaact
     9961 tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa ataaatacct
    10021 aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac ttcttcttct
    10081 tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat attttggatt
    10141 tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc aaaaactcct
    10201 actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat ccgtatgagg
    10261 aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat atcttctaca
    10321 aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc cttattgacc
    10381 aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag tttattttta
    10441 cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga attcatcagg
    10501 ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa tcccacgaat
    10561 gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc gatatattca
    10621 tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga tacaaaatgc
    10681 gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg cagaatatct
    10741 gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg ttcctgatag
    10801 acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg gtagcgaatg
    10861 gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg gcctggtcga
    10921 tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct tcgtaaaacg
    10981 ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg ttgaaacaag
    11041 agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata cccttctact
    11101 cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc taaaaagtcc
    11161 attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag aaaagcttca
    11221 tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat aactacacct
    11281 gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt tttgaataaa
    11341 agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt tttataagct
    11401 tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt tgcttcactt
    11461 tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc cattgtgcca
    11521 aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata attcaatact
    11581 tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa gcgttcgggg
    11641 tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt gatttctaac
    11701 acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac gattttgttt
    11761 ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa tcccatttga
    11821 tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata gtcagtgatt
    11881 cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg ttttccatta
    11941 ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga aatcgattca
    12001 gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa tcgtttgatc
    12061 atttcttgtt ctgaaagaag atagccattt gctgttagtg gtaaagatgc agataaattt
    12121 tctaaaggta tttggcttcc atctttgata tgtttcatca cttcaagtgc aaaaagttct
    12181 tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc ttttatcaca
    12241 tactgatacc attcaggcaa ttgatcaggc gtgttttcta tcaagccgat ataaaaattt
    12301 ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc ctctttattg
    12361 aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc ggtttctgtc
    12421 atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt tatcagctgc
    12481 tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc ttcataagta
    12541 taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat acgatagaat
    12601 tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta gccttgtttt
    12661 tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga ataagaagta
    12721 attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt taaaattcac
    12781 tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg aagttgatta
    12841 ttccacttca tttgcttgta atctgtagga atgagttctt tcgcaagaac tcaaatttta
    12901 ttgagatttg gtctcgtttc tttacattca aagaaaatct ggtaaaatta agatagaaaa
    12961 gaggtgttct ttatgcgata tatcgttaca ttattctggg ctgtcgttct aggtcaagta
    13021 gtcggttata ttggtgctgc attaacaagc ggaacatatg attttacttt gacaacaatc
    13081 atttcattca ttgcgggtgt cattatcctt ttgattggtg cagttgcgcc aagaaaagaa
    13141 acctctgccc attcgtaaaa aaacagccaa gagcttgaga tatctcaagc tcttttttat
    13201 tttctgctcc tcaccaccat tataatctgc tgcgaagaac tttacgaaag ttgaataaag
    13261 aaagaaaaat atttgacaga acaaacaaac ttgtcacaat aaaaacagca ctgtaattat
    13321 aagcatgcgc gactgtcgat cctagcatgg gacctaaaac ttgtccaaag ttgctaaaca
    13381 tctgattgta gctatagatt cggctgactc cttcttgagg cgtaatctta ctgatcagtg
    13441 tattgacaga aggcatcaaa gcgccagttg aaaagcctaa aataaagcga agtataccta
    13501 attggagcgg tgtagtgacg aatgccatag gtaaataaca gcaaaatgat aaaatgagtc
    13561 ctccaattag aactttttga ttcccaatct tatcccctag ttttcctagt ttcggtgaag
    13621 aaaaaacagc ggaaacacca gctatcgaaa caatcaagcc gctgacaaaa aggatatttt
    13681 ctgtacttcc gctcaattca cgaatatata aggtcaaaat agggctgatc gtagtgattc
    13741 ctatctgtaa aatcaaggtc gtaatgaaga gaccgatcaa aatagagaga tgatccattt
    13801 ttgaaaaaat ttctttggtg cttatcagat cttttttctc gatcggatgg aaatcttctt
    13861 tgactaagaa aatggtcaat accgttgtga tcatcaacaa agcccctgtg atcaagaata
    13921 cattttccat accaaaccac tgagccagtg caccgcccat tgaaggtccg atcagatttc
    13981 cagcaatcgc tccggtagac aatgttccta acgcccatcc gctcttttct ctaggtgcct
    14041 gtgaagcaat catcgctgtg gcatttggaa tatatccaga caaaattcca ttccaaaacc
    14101 gcatgatcaa aagccaatac gcgttcggta caaaagccaa agatcccatt gttaatgtca
    14161 tccctgctgc tgcacggatc atcatgagtt ttcgtccttt tcgatcagct agattccccc
    14221 agattggcgc aaccacggca gatgcaaaag cagtcacaga gattgctagt cccgaaaaca
    14281 attcgatttg atcttttggt gttcctagtt gctcaacata aactggtatg aatggcataa
    14341 ctaaactaat acttgcacca gtgaaaaaac agccaatcca cgcaatcact aaattttttc
    14401 tccaattaat ctccatctat catccctctt tcgtctcttt tatcatacct attttcgaat
    14461 aaacaagcaa cgatctcgcc agtaaaaaga gaaaaatccc gcaaaggtca ttttatatgt
    14521 aacgttcttt ttatgtaaaa caaaaagaga tcagaacaaa tactgttcca atcccttctc
    14581 tttagttaaa ttgttgctag aatgatgaag ttgataatga ataaaatatc aacgatccac
    14641 aaagcaacag aaacttcatt gtattttcct ttgattactt tcacaattgt atagaaaata
    14701 aatcctgctg caatcccata ggaaatgcta tagcacaatc ccataaagat cgatgcaaag
    14761 aaggccggta aagcttcttc catatctagc catttgatgt ctgcaaaaga agccatcatc
    14821 attactccaa ctaaaatcaa tgctggcgca gttgcttgat ttggtacaat ggcgatcagt
    14881 ggagagaata aactacttaa tgcgaatagc actgccacta ctactgaagt caaaccagta
    14941 cgtccaccgg ccccgatccc agctgcagat tcgacataag ttgttgtatt cgatgtacca
    15001 aagatagccc ccacggaagt agcgatcgca tcggcaaata gtgctttatc catttttgtt
    15061 ttgaacccac gaccatcttc caacgcgatc tcgtcttctt ttgagaaaat gcctgtgcgg
    15121 cgtcctgtac cgataaatgt tccgatcgta tcgaaagtat cagataaact gaatgcgatg
    15181 atcgtcatca atacttgagg aatcttggaa gaatcactga ataaagattg catgccttca
    15241 gaaccaaatg ctgcaccgaa agttgttcct aattctttaa atgaattacc taacgaattc
    15301 gtttgccaat caatggaact taaatcaaca acacccataa aaataccaag gactgtggtc
    15361 gctacgatac cgataagaat agccccacgg acatttttta caactaaaat ggctgtcagt
    15421 accaaaccaa tcacagccaa taagatagga gcgttgtcaa agttggctaa cgcagggacg
    15481 attccaccat ttatcgtaac atttgtcgct tttccgcctt caacgacaga gctcgaaata
    15541 gctgactggt ctgctgaaaa agataagaat cctgcatttt tgattccgac atacgcaacg
    15601 aaaataccga tcccgccacc aatcgcatgt tgaagacttt cgggaatcgc ccgaataatc
    15661 atttttcgaa tatttgtgac agtaataaaa atattgatta atccgcaaat aaacaccatt
    15721 gccaaggctt gttgccaaga ataaccaagc ccaaaaacaa ccgtaaaagt gaaaaatgcg
    15781 ttcaatccca taccaggtgc ctgagcatat ggtacatttg cgaacaatcc cattaccagc
    15841 gtaccaataa tcgatgcaat gattgtcgct agaaatactg cttggaaggg catacctgat
    15901 gctgacaaaa tcgtcggatt tacgaataag atataactca tggcaaagaa tgtcgttact
    15961 cccgccatta tttcagttga tacatttgta ttgtttttct ttaaaccgaa aaacttttcc
    16021 ataaaaagtt ccactccctg aatttttt
//
LOCUS       DAZUMM010000062        15467 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000062 DAZUMM010000000
VERSION     DAZUMM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.062"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(362..1480)
                     /locus_tag="ACG8E6_001953"
     CDS             complement(362..1480)
                     /locus_tag="ACG8E6_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062359.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1951..3258
                     /locus_tag="ACG8E6_001954"
     CDS             1951..3258
                     /locus_tag="ACG8E6_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFS5062360.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3377..4576
                     /locus_tag="ACG8E6_001955"
     CDS             3377..4576
                     /locus_tag="ACG8E6_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS5062361.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4603..5871)
                     /locus_tag="ACG8E6_001956"
     CDS             complement(4603..5871)
                     /locus_tag="ACG8E6_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFS5062362.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(5926..5992)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6034..6680)
                     /locus_tag="ACG8E6_001957"
                     /pseudo
     CDS             complement(6034..6680)
                     /locus_tag="ACG8E6_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7088..7591)
                     /locus_tag="ACG8E6_001958"
     CDS             complement(7088..7591)
                     /locus_tag="ACG8E6_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5062363.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7714..8478)
                     /locus_tag="ACG8E6_001959"
     CDS             complement(7714..8478)
                     /locus_tag="ACG8E6_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFS5062364.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8585..8860
                     /locus_tag="ACG8E6_001960"
     CDS             8585..8860
                     /locus_tag="ACG8E6_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFS5062365.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9576..10529
                     /gene="yidC"
                     /locus_tag="ACG8E6_001961"
     CDS             9576..10529
                     /gene="yidC"
                     /locus_tag="ACG8E6_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFS5062366.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGGAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAAKPADNKQATKQIKQPKNSTNNKGRNAGRQQKRK"
     gene            10651..11860
                     /locus_tag="ACG8E6_001962"
                     /pseudo
     CDS             10651..11860
                     /locus_tag="ACG8E6_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(11905..12305)
                     /locus_tag="ACG8E6_001963"
                     /pseudo
     CDS             complement(11905..12305)
                     /locus_tag="ACG8E6_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723846.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(12552..12986)
                     /gene="tnpA"
                     /locus_tag="ACG8E6_001964"
                     /pseudo
     CDS             complement(12552..12986)
                     /gene="tnpA"
                     /locus_tag="ACG8E6_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005576373.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(13164..13530)
                     /locus_tag="ACG8E6_001965"
                     /pseudo
     CDS             complement(13164..13530)
                     /locus_tag="ACG8E6_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            complement(13544..13829)
                     /locus_tag="ACG8E6_001966"
                     /pseudo
     CDS             complement(13544..13829)
                     /locus_tag="ACG8E6_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(13838..14029)
                     /locus_tag="ACG8E6_001967"
                     /pseudo
     CDS             complement(13838..14029)
                     /locus_tag="ACG8E6_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14029..14505)
                     /locus_tag="ACG8E6_001968"
                     /pseudo
     CDS             complement(14029..14505)
                     /locus_tag="ACG8E6_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            complement(14973..15326)
                     /locus_tag="ACG8E6_001969"
     CDS             complement(14973..15326)
                     /locus_tag="ACG8E6_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062367.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
BASE COUNT         4557 a         3263 c         2783 g         4864 t
ORIGIN      
        1 aacggtgttc cagaagtagc ttcttcggaa ataagccgaa atttcacaaa aatttgagaa
       61 ataatttttg tcaatctctt cttatttctt gaagctgacc acttctgtca caacctcttc
      121 cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaccaaaca aaaaatatga
      181 agagctattt ttgcttgctg ttcttattac ttgatgcaga acagcttttt cacaacctct
      241 catacacggt gtttaatcag ctgaccttcg gtattagctc aaaaaacgga aaaatatgtg
      301 aaactatttt tccgtttgtt tctctaatac tcaggtcaaa acactgattt cacaacctct
      361 tttaataacg aagaatcgct gtcagactct tttcatctgg cgcatctttt tgatccaaaa
      421 taaacacttt cccgccatta atgatcacat catctgctaa tgcattcaac acttgacgcc
      481 gatcatattc tgtttcagga tcttcaccga aaccatcagc taagtttgac gtagcgataa
      541 ataaatgtga cacttttcct tctttcgcag ctggaacgat atcaactaac tgatcgatga
      601 atttcttatc taagagttta ttgtattctt ttgattcttg agctgcgagt tcacttgaca
      661 gcttcttcgc cccttcttcg atatctctaa aggatagctg cgcaggagaa gcagaaatcg
      721 caacgtctgc acaataatag ggatttttag caattttttt gaacatcgtc tgattttctg
      781 gtaatgcaaa taaataaatc ggccattgtt caggattttc gaaagaatcc ttcaagtacg
      841 tatcaacagc ttggtaatag ttgacccaat cgatctctac ttcttcatct tttggattta
      901 cgccatggaa agttactcca tctcgggctc cgcctcctcc attagaagag aagttcaaac
      961 cgccacctgt cagttcagat cccaatgcag taacgacatc aaccggtgcc ccttctggta
     1021 gagatacttc ttttacttgt ttgttgctga cctcatacaa tttcatggag tcgcggttca
     1081 atcccaataa ataataagaa tagttgaact gcccattttt gataatagcc agtaaataag
     1141 gaagatcact tacatagtat tgatcgtcta ccgggatact caaacgatgg atataggtct
     1201 ggtcagcagt tataatgacc gctacactcg tagttgcact acgccaaaac gaaggatctc
     1261 ctaacaaatt ttcaattttt tcttggaacg gggaccagtc atgttcggaa tattttttct
     1321 caaaacgttt ctttgcttct ttcgcaaaat ttttgaattt gatctgatct ttttccacat
     1381 cttgatgggc gacatgcgta ttcagcatga tcgtgacgaa tggtccttta gcgccttctg
     1441 aagtcaattg tgtcaataat tctttatcct tttttgccat aaacgtaccc ctcctaaaaa
     1501 aatcattcct ttgtttctat ctaaagtcta ccacaacact atttttccat acaataaaac
     1561 gtatctaaaa aaataaaacg gtttctacgg ttttaaattt agctattttt ttaagttatc
     1621 ttcataattt cctcaattat ttagccccag tcttcaacta ttcttcatga aatgctgata
     1681 aactaagaaa aagaataaaa gaggtggatc ttatgcattt gattatgatt ttcagtttat
     1741 taagtattat cagtttattt gctttcagcg cttcccgctt attagatgcg gaaacacagc
     1801 ctattcctgt aaaagtacgt aaaaacgacc gctaaacttc cataacgcgc gttcttatcc
     1861 taaagttttt tcagaaacat tgaaactctc tcccctctct gataaaattt taatttgtag
     1921 cgaaacaaat taattacaag gagatcggat atgtccataa aaaaaacacg tctagtccta
     1981 ttattggcta gtctcattct ttgccttgct tcttttctac cagctgttgc tgtgcaagct
     2041 gaagatacgt tcaaagtaaa tgcaaaagca gcttttgctg tagatgcgca aagcggtaag
     2101 attctttatg atcaagatgg agaaaaaacc atggggatcg cttcaatcac aaaaatcatc
     2161 ggtctttaca tcgtccttga tcaagtaaaa gaaggaaaac tgtcatgggg tgataaagtt
     2221 tcgatttcag attatgcaga aaatttgagt atcaccccag atttatcgaa tgttcctttg
     2281 cataaagaaa acacctacac tgtcaaagaa ttattcgact ctgcaatcat ccagtcagcc
     2341 aatgcttcaa tggtggcctt ggcagaaaaa atctctggca gcgaagctaa attcactgaa
     2401 cgaatgaaag aacaattgaa agattgggga atcaaagatg caacaatcgt taatgcatca
     2461 ggattgaaca actcttatct tggcgaaaac cgaccagaag gcactggaga aaacgacgaa
     2521 aatcaaatgt ctgctcaaga tgtggccatc gttgcccgcc atttgatctt ggatttccca
     2581 gaaatactgg atgtatcaag tactacgacc caaatgtttg gcgaaaatac gcaatcacca
     2641 gtggaaatgg tcaactggaa ctggatgctc ccaggattcg ttaattacaa ggaaggcgtg
     2701 gacggattaa aaactggtac gactgagctt gcaggtgctt gtttcgttgg tacgatcact
     2761 aaaaacgatc aacggatcat cactgtcgta ttgaatgcgg acggccatgc ggaaaacccg
     2821 agtgcccgct tcaatgaaac agaccgtttg atggactact gctatgacaa ttggtcagaa
     2881 aaagagctcg gaaaagctaa cgcaagtatt cctagtctaa aaaccattga tgtaaaagac
     2941 ggaaaagaat caacagttcc tgttgtgctt aaaagccctg taaaagtatg ggtacgaaac
     3001 gatatggata ctggcaaatt aacgatcacg ccaacacttg acaagaagca agtcactgac
     3061 aatgagatcc aagcacctat ggataaaggt acaaaaatcg gtaccgctac gatcaccttg
     3121 gcagacgata aattaggcta tttagaagac aacaatgcac catcaacaga gatcattacg
     3181 gataaaacag tggaaaaagc gaatatattc gtgattggct ggcgacgcgt cgcagaattt
     3241 ttcggcaact tgttttaatt gaacaataag aaaaacatct ttccatcgga aagatgtttt
     3301 tttgcttttt ctagtttaaa taacagcttt tttcatctat tttcgttata ctgtataaga
     3361 aaaaggagtt gaatttatga atctaaccag agatctgatc tttttagttc tctatcttat
     3421 tggggtattc tctatcgttt attggcgaaa acagaagaag gatccttcct tgttctttta
     3481 cggcagtacg tggacattta cggtaatctt aacttatttt gtcgtgttgg aaatttttta
     3541 ttcgaccttt tattttttga ttccattgtt gttctttagt atctttgctt tttcttattt
     3601 cacagaaaaa cgaaggctac tgaatggtct tttattcaat gttttactca tcagtttcgg
     3661 catctacttg tttgtcttgc tgtatgaaac gcaaaatatt tttcttggcg gactgatcgc
     3721 cttgatcacg attccattgc tacttgtcct tttatttggt atttatggat tgattgtttt
     3781 tcttttttgg aacggggtca cagttttacg tagagaatcg cattcattag ctaatctttt
     3841 aacgttgata ctagccattt ttcttacact gtttcttgtt tttgactttt tcttgttaaa
     3901 atatttgccg caatggataa atgctttgtt tttctgcgtt cccctgatct tgatctatct
     3961 atttatcgta ttctacaact ttttgactgt ctcattcttg tatcagttca accggcctcg
     4021 atacaatcaa gacttcatcg ttgtgttagg cgctggattg atcaatggag aaacagtttc
     4081 tcctcttcta gccaagcgaa tcaataaggc aattgctttt tatcgggctc aaagccgtgc
     4141 tacattaaat ccgcccatct tattgatgtc tggcgggcaa ggagcagacg aaaaagtacc
     4201 agaggccatt gcgatgaaac aatatgccat ggaacaagga atccctgaaa gagacattct
     4261 tgtcgaaacc aattcgacaa ccactttgga aaacatgctc tattcaaagg aaatcatgga
     4321 tcagcaaatg aaagggccat accgagcgat ctttagttcc aacaattacc acatttttag
     4381 agccggtctt tatgcgcggc aagccaaatt gaaagcaaat ggtattggtg caaaaacagc
     4441 tttctactac ttgcctaatg cgtttttaag agaatacctc gccatcttga tgatgaacaa
     4501 aaaaagacat atgatcattt gcggattgct tttcgcaggc tccgcattct tatcgctgat
     4561 tactttactt ttctaaaaaa acaaggtgtg acaaaacata tgtcacacct tgtttttttc
     4621 tttcattctc ttaactattg gcaaaaagag tatccctttt ttgaccgcta cttgctgagc
     4681 agaataaata ccgctgttgc ctgaacacat aaagctgatc aagcagatca taaagaagta
     4741 tactgccgct tgtgaaccga acaattcgat ccccatcaca aagcaggcaa tcggtgtatt
     4801 tgtcgcacca gaaaatactc cgataaagcc caatcctgca agaaaaggaa cagaaacatg
     4861 gagcaaaggc gctagtgctg caccaagcgt tgcaccaatt tcaaacaatg gtgtcacttc
     4921 tcccccttga tatcccgcgc ctaaagacaa aactgtgaag aataacttcc caataaaatc
     4981 atagacatgt gcctgtccat taaatgattc ttgaagtaac ggaagactca atccgagata
     5041 acgttgtgtt tgcaaaatca atgctgctgc cacaactaca gctccaccta tcgtatttcg
     5101 acaaacagga tgaggaatcc attgtgcata tcgcgctttt aaaaagacga tcgaacggct
     5161 gaatacccaa ccaatcaaac caaaagcaat cccagcaaca aaaactttga agaacagcaa
     5221 catactccat tcaggaatca cacccatttg gtaatgtaga tgagaaacac catagctttc
     5281 tgtcatgaaa tttgcaaaca gtgcagcaaa gaagctaggg aagatcgctt ctgttcggac
     5341 tttcccgatt gccaacactt ctaatccaaa taatgttcca gctaaaggtg tgccaaatac
     5401 agaactaaaa cccgcactga ttccactgat aatcaagatt tcccgttctg atttatccaa
     5461 tcggaacaaa cgtgccgtat tatctgctaa cgctcccccc atttggactg ctgttccttc
     5521 acggccaaca gatcccccaa ataaatgggt agttatggtt ccaaacagtg taagtggaat
     5581 aagccgcaac gggatttttt cttctccacc atttccttga tcgatcacta agttgtttcc
     5641 acgactagaa agtccgccgt gatacgcata gagataagca aaaagcgcac cactaactgg
     5701 tagtagacaa aggagccaag gatgtgatag tcggatatct gtcacaactg tcaggctatt
     5761 taagaaaaaa gcagacaagc tccccatgaa tataccgaga atcgctgtga tcaccagcca
     5821 cttcagcata tacactaaaa tgcgtactgg actcggtaat tgtttttcca taaaaaattc
     5881 ctcctcaata aaaaaacgcc tactaaagaa aggggctgtc cccagacttt agtaggcgtc
     5941 attagctatc tttcgttagc atttaaaggc gaacaccatc acctattcac ttccattaaa
     6001 agatagcata tttttctgta aaaaacaata aaattatcgt tctttcttta cacgttcagc
     6061 aaattcatag aaaggagcca atttcgttaa ctgttgatta cattggtcac tatcatgaca
     6121 aatataattc ccctttttcg tatacgtgcc atcagaacct gctttcgttg tagaaagaaa
     6181 aagcgaaaca ttcgatgttt tctgacagat tgcacagatt cccttattta tcgttggtga
     6241 taaagtaccg ctaatccctt ttaatttttc atcttcgtaa tagaccatga attttttctg
     6301 cgtacctgga tcattccagc cgatgtacgt atgttcttgc aagtcgattt cattccaagc
     6361 tggttgtttg agttttttca cttttcggaa taatttctcg atttgtggtt tgctaggctg
     6421 cataaacggc tgaacgattt gcttcagctc ctccaatgct ctttctgctt gcgcttttgt
     6481 cagttttacg tctaaaactg tcgttaaaaa tgtatcgatt ctggcagttc gtgatctaat
     6541 atgtctcgaa tcttttcagc tgctagtgcc tgaactgttt gtaccgtttt tggatcattg
     6601 acagatgagt acgcatttaa taactgttct acttctctta ttataaaaaa atattggtat
     6661 ggttttattg tttgttccat gattttaaag tcctttcttc taaccaaagt gcatactttg
     6721 tagaaaaaag tgatcttctg caaagtatgt gttacctgaa gatcctggac tcttttaaat
     6781 aagatacgtt ttgcatctta tcatcacccc ctttaatgct tattattcta tgtacatgat
     6841 tcattcgata aataaaagta aaccgtatct tgaacagata gtcaactgca aacagaaaaa
     6901 aggactgtga caaaagtcgt gtcaaagtcc cttgtttcga atgaatggtg ttcaaaagct
     6961 tgcatctgcg gtaataagcc gaaacttcac aaaaatttga gaagtaattt tcgtaaattc
     7021 cttcttattt cttgaagctg accacttctg tcacaacctc aatatggtct attattggtt
     7081 ttacctgtta aatcgaggat tctttcccaa agtaacgttg ccatctctta cgcatacttg
     7141 cagacatggg ctgagccgct tctttgactg cgcagtattt gtccacgaat gtcacaatat
     7201 atccttcttt atacttaggc ggtgcgatcg ttgcacccca catgtgcttc aaaatgatat
     7261 ctcgctcaag atccgacaaa tcggtgattt tttctgcatt ctttacagca attcttggat
     7321 ggatatacgc atgtgtcccc tcgtcaaatt tcgtcgtacg ccaatcatag tagaacagat
     7381 catgcaaaag tccagcacgc gctgttgctt tagcattgcc accccatttt tttgcgagca
     7441 gataactata gtaagaaaca ctgatggaat gctccaaacg agtggaatga acatgctgtg
     7501 tgtaattcgc taattttttc actgcttctg tttctaaaag atcttgtaca taagacatat
     7561 attcttcgtc ttcacgccat ttttctgtca taattatcga tttgccacct ttttgaattt
     7621 cattttcttc tgaaattgat ctaagtatac caccagtgtt cggattattc tagtcgctct
     7681 agataaaata gctaaagctt aatagttcct taatcataaa gaaaaatgat aaagaacgat
     7741 ccctgctgcg actccgacat tcaatgattc cgcatttcct ttcattggga tatatacgtt
     7801 ctgatcagtc gaagacaaga tttcctgact tactccctga ccttcgtttc ccatgattat
     7861 agcgaaatcc tctgtttttt ctactttgtc taatgcatgt gcttcttcat ttaattctgt
     7921 accgtacacc gtaaaccctt tgcctttcat tacagggatc acttctgcca aatctttttg
     7981 taacacaggc aaatgataat tgcttccctg catgcttcgc agcacctttg tactataaat
     8041 atctgccgtt cctttaccta ataaaacacc agccatacca aacgcatctg ccgtacggat
     8101 catcgttccc acatttccag gatcctgtac attatctagt aaaagccagc ctcctgaaaa
     8161 atctggcgta tcttcagatg ttaacgtcat cactgctaag atcccttgcg gtgtaggtag
     8221 atctgacata gcggtcatga cctcatctga aactagaaaa gtagaaatct ctttgtttac
     8281 agccagccag tctccccatt ctttcatacc tctctgtgtc acaaaaagtt cttttatttc
     8341 tgcatcagac gacgcagctt cttcgatcag atggaaccct tccaaaagat attctgcctt
     8401 ttcttctctg tatttctttt tatgtagttt ttttaattct ttgattcttg gattttttac
     8461 tgattggatc tctttcacat tcctcacctc aactgattat atcatggaat aggtgcttga
     8521 gcttgtttca tcctaaaaaa atcggtatca ttgatttata aaacaggaaa gaaaggtgac
     8581 gaacatgcgc aaagttaaaa tgaatgtcca aggacgtgtc cagggcgtgg gatttcgcta
     8641 tatgaccaag atggttgctg atcagttagg cgtgaccgga acggtaaaaa acgaagacga
     8701 cggctcagtc acaatcgaag caatcggaaa tgacgacatc atccaaaaat ttatcgaaga
     8761 agtcaagaag tctccgagcc caagtggacg agtgcagtat gtggatgtcc aagaagatcc
     8821 gatgattgag gaacgaaaga aatttgatgt ggtgggataa ggttgttttt gaagcgcttt
     8881 gcctcgagta ttaaggagaa acattttcca aatagcttct catattttga aatgtaatga
     8941 cttaataccg agaggcagct gataaaacac cgtcaataag gttgtgaaaa agctggtctg
     9001 catcaagcat taaggactgg ttgtgaaaaa tagctctcca tatttttcaa caggctggac
     9061 ttaatgccgt agatgcaagc ttttgaacat cgtcgataag gttgcgaaaa agctgatctg
     9121 catcaagcat taaggactgg ttgtgaaaaa gtcgcttagt tttgagtatt aaggaaaatt
     9181 ttgtgaaaat ggctttccac atttttgcat aaatttgact taataccaag aaactgactt
     9241 ttgaacatcg tcgataaggt tgtttttgag gcactttgcc tcaagtatta aagacaggta
     9301 gtaaaaaagc tgatctataa cttgcaataa atttgcttaa cccatatttt ccagacagaa
     9361 caaaaaagtt tttagttttt taaataagaa agaaacactt ggaatcactc ttttctttct
     9421 aagtatttct ttcttgattt tttgtctttt ttcaaaattt ttcgctacga aaaagaacga
     9481 atatttatat gaaatttcta tgaagttgat tgaattccat gcgctttttt agtatagtta
     9541 agtttgtgta aaaattatag aagaggaagt attctatgaa taaatggaaa aactggttat
     9601 tgggatccgg acttgtcagt atcctgctct ttttatctgg ctgtgtgcgg atggattcaa
     9661 acggaaaccc tgacacttca ggaatcgttt atcgagtact cgtccatcct atgggccaag
     9721 cgattacata tctagttgag aatttcaatt ggtcttacgg ctgggcagtt attgcaatga
     9781 ctgtgatcgt tcgtatcatc atcttgccac taggtatcag tcaatcgaaa aaaacaatga
     9841 tccaatctga aaaaatgcaa gcgttaaaac cacaagttga agcagcacaa caaaagctaa
     9901 aagctgcaac aacacgtgaa gaacaaatgg cagcacaagc agaaatgcag caagtatatc
     9961 gcgaaaatgg attgagtatg accggcggaa tcggctgtct gcctctattg atccaaatgc
    10021 cgatcttctc tgccttgtat tttacggctc gctacacaga aggtatccgt gaatcatctt
    10081 tttacggcat cgatctaggc tcaccaagta tggtgcttgt ggccatcgca gggggcgctt
    10141 atctgcttca aggatatatt tcaacaatcg gtattcctga agaacagaaa aagacgatgc
    10201 gtacgatgct gatcgtctct cctgctatga tcgtcttcat gtcgatcagt gcgccagccg
    10261 gtgttacact ttactgggta gtcggtggtg tgttcagctg tctgcaaaca ttcatcacaa
    10321 acgttataat gaaaccgcgt ttgaaagcac aagtggcaga agagttgaaa caaaacccgc
    10381 caaaacaagt cgttactcca agaaaagacg tgacagaatc agcagcaaag ccagctgaca
    10441 acaaacaagc aacaaaacaa atcaaacaac caaaaaacag tacaaacaac aaaggccgca
    10501 acgcaggaag gcagcagaag cggaaataaa aatatatgac aaaaaactgt agacaaataa
    10561 aaaaatttct ttgttctaca gtttttttgt tacaattaaa gaaaacgttc gcatttcgag
    10621 gaggaatgta taatgatgtc caaataatca atgtatagtc gaatggaaga agacaaaatt
    10681 tcgctagatg cacttgttcc taaaaatcat ttagtccgta aaattgacaa attattgtca
    10741 tttgatttta tttatctcat tgttgaatca acttactcga ccattggtcg ccctagcgtt
    10801 gatcctgtcg tcttagtaaa attagtattc attcaatatt tatttggaat ttgttcgatg
    10861 cgtcagacca ttaaataagt tgaaacaaac ctagcttatc gttggtttct tgggcttagt
    10921 ttaattgata aagttcctca tttttcaact tttggtaaaa actatgcacg gcgtttcaga
    10981 gaaaccgatt tgtttgaaca gatatttatc attattctcg aataagcagt tgatgctaga
    11041 tttattcatg aagaaatttt gtatatggat tcaactcata ttaaagcgaa tgcgaacaaa
    11101 cgtattttta caagagatat ggcacataaa gaagccattt tataccaaaa acaattagaa
    11161 gaagaaatca atgaggcaag aattgccgaa ggcaaacgtc cttttggttc aatcattcgt
    11221 caggaggtgg tcgaaagaaa atttagcatc gcagatccag aaagtggtta ttatgtaaaa
    11281 acagaacgag aaaaacagtt tgcttattct gctcataccg ttggtgatga aaatggtttt
    11341 gtattagatg tcatgattac gcctggaaat ctacatgata gccgaatgct cgtgccacaa
    11401 atcgaacgcg ttaaatcacg ttatggtgta gctgctaatg ctgcgtacaa aacaccttgg
    11461 aacgtcaagt atttaatcga acgaaaacta cgacccattc tcccttatat cagtttcgca
    11521 caactactcg agatggctat aaaaaatatg tatctaaatc ttttatttgt gaaagttgtc
    11581 cattattgaa aaattgtaca gaaagccaaa aacaccaaaa agaaatacat cgacatattt
    11641 gggaagacta cttagatgaa gcagaacatt tacgccatac ggtgtacaat cgtgaaaaat
    11701 ataaaaaaag aaaagaaacg attgaacgca tttttgcaga cgcaaaggaa aaacatggaa
    11761 tgcgctacac caagtatcga ggcttggaaa aaatcaggtg catacaatgc ttgtttttgt
    11821 cgcaatgaat ctgaaaaaat tagctacttg gcagtgggag catatgtcgt tatatctttt
    11881 attatacaaa aatagacaaa agtatcagtc tacactctta gtaaagagga aactgatgct
    11941 tttgtctaca gtcttagaga gtgggacaat agttttttct tctttacgtc ttttcctctc
    12001 catgtatttt cctaattttc ttaatttaac gccatgagtg ccaagccaat gtcaatcttg
    12061 gctccttgtt tccttcataa gtggaaatcc gtttccgata gatcgaacct tctttttcct
    12121 ctaaaagttt ttttacattc tgctttgaaa taacgccatg tattattcac taaaatttgt
    12181 cggtttcgct cactttttgc tttcgtacat tgactacgaa caggtcagca ccgaccactt
    12241 tcacattcgt aaattttgaa gacacgtata aatccacttt tatcttttct tcgactaaag
    12301 cgttgaccgc aaaagcgaca tctgtttcag aaatatattg ttccatatct aatggtaaaa
    12361 ttttttggtt gatggtataa tttttaaaca taaagcactc gcttcctttc tgattgggtt
    12421 tggtcacttt aatcctacag ggatgagtgc tttttgtgta ccttaaaatc agtaaatatc
    12481 catccgtgaa aacgggtggc tttttaacag ccctagaagg gctcaccact cacagcccta
    12541 tctctcttta actatttaaa tggatctaca tactcttgtt tacttatact gtctctaatt
    12601 cgatcatctg cttcttgttc tcaaatatat atttcaattg ttttttgatt taacccaacc
    12661 gtactcacgt agtatccttt ggtccaaaag cttttattcc catacttata tttcaaattt
    12721 gcatgttttt catggattaa ggttgcactt cgtcctttta agtagcccat aaaactagaa
    12781 atactaatct ttggcggtat tcttactaac atatgaatgt gatcaggcat tgcatgagct
    12841 tcaatgattt caacatcttt cctctcacac aattttctta atatctggcc aatacttact
    12901 cttaactttc cataaatcac ttttcgtcga tacttcgggg taaagactat gtgatactta
    12961 caatcccatc tcgtatgtgc taaactttta tcgctaaagc tatttcgtcc acgcgtagaa
    13021 acgcgcgttt ttttgtttcg tacgctacgc caacgaaact gactaaagtc aataaaaaaa
    13081 gtggggcaat aatcagattt ttttctgact attgtcccac tctcttttta ttactcttca
    13141 tcttctacta ttccactaat taattagttg acagatacaa tgctttcaca tcctgcacga
    13201 acaaactcgg gctctgaacc aatcacttgc gagttgataa attcaatgac cataggaaga
    13261 ttaatccgac ataaagttta atctgttgtc cttccattat cagtttagaa atgatattag
    13321 taggtgtacc gcctaaaaga tcgcatagta ccacaaaatc atttaacgaa tctatgacag
    13381 taagagcttt tgtctgtatg tttcagtgcc atcttctggt aataaaggta acgtgtagat
    13441 attctcttga ggtcccataa tcatttttgt actttttttc aattcctcac aaaattttcc
    13501 gtgactaatc aatatcaatt ttctactcat ttttggctct cccttatttc gaaatcacac
    13561 acaatgcaga taaagccaca cacaagtaat acgataaaaa tcgctcgagt tgggtcatat
    13621 ttttctttcc taacaaccaa taaacacctc gacgatccca gcgagtaaga ttttagataa
    13681 tatcatatcg atatagtttt gcatattcaa tgtcacatcg ccaatcgttg gtaccataaa
    13741 aatttaatat caatcattgt tgcaactaat gctcctacca tgaacactct taataaagtc
    13801 gctacgtccg ttaatacagt taagcgagat tacactagat gatctgttcc tgccaaggca
    13861 agagccagaa gcggttgcct gcccatttgc ctttggtgtg tcggagctgt ccgataaagt
    13921 tcaccgcccg atccgctctg gctttgtcgt agtgacattc cgaaagcatg aatcggcttg
    13981 gtgtgtattc ctttaatttg ggatagccct ttggtctggt ttctgccatc agctaccacc
    14041 tcccaacaga gcctccatcg catcttctgc tccgacacca ccgccatcac cggcaatgat
    14101 acggcttctg gacgaaggcg tcagaccgaa ctgctcggaa aatcggttca taattttcag
    14161 ataggtctgg gcaataaaca cctgtggtac ctgctgccaa tagccggata gggttttcac
    14221 gatgataccg tgctgggtaa tgaattcctc ggcttccttc cagcgggcgt aggcttggca
    14281 ctaaccggca aaggcagcca tatccacatc tgtcaagatt ccgatggcct ccatctgctt
    14341 tgccagacgc ttccattcct tctttgcctc tggttccagc cacttcggac aggtcggtgc
    14401 cttcttttcc ggcgttgact cttttgcatt gagtgggcga ttgcccggat ttccctccag
    14461 cttcttcatg gctgtcgggg ttgattttct tcctctggtt gccacagtcg tcacctccct
    14521 tcaatgtatt tctgccagta gacagagaac agagccaagt cgaaacctga ctctcttctc
    14581 tatctgctaa cttgtatttc agtccactct tctgatgaat cgcacccgca ggattttcaa
    14641 gatgccgcag gcaaattcca agaagtttct gaccgcatca taaccatcgt agccgtgtag
    14701 ccacatcctt ccacctcgcc gccgtcctcg tcgctgatat aaatgattct gtattcgctc
    14761 atctgttgtt ttctccagtc ttttgttttt ttgtagtaca tatatcactc taaaagtgat
    14821 tattatcaag tcgtttgaaa gccataatgt acacaatcat tcgactggtt tttggttcat
    14881 aattgaccgc caaagtgagc aaaagaaagg gacctctgtc tgtttcagaa gtccctttcg
    14941 gtatttggtt cttagtggtt caggctgtat tctcaaacct catactcatc ttcgaggtct
    15001 gcctctgcaa tgatctggcc gatgctgtcg taacgctgga cctgcatctg gatggcatcc
    15061 ttgtctgctc cctcgtccat catcttcagc agcttgtctg attccttttt gtaggcccag
    15121 aaaaggatct cctgaaggct ggtctcctgc tttttcttgc gaagggcgac cttcttctct
    15181 tccaccgcct cgttcaactt ggtgaactct gcccaagcaa ggataccgtc tcgcttgatt
    15241 gcgatctcca catccttcag ctccgggatg gtctttgcac ccttgatctc cttcaaatat
    15301 ttctttgcct tttcgctcat tgccattagc gtttcctcct tgtttttggg attttcccct
    15361 ttggttgtgt gtatattgcc atagtctctc gactatatca agtcaatcca caggcatata
    15421 ctacacaatc atttggagct gtatttgtat acattatgcc gatttat
//
LOCUS       DAZUMM010000063        14926 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000063 DAZUMM010000000
VERSION     DAZUMM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.063"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..1936
                     /gene="yfcC"
                     /locus_tag="ACG8E6_001970"
     CDS             197..1936
                     /gene="yfcC"
                     /locus_tag="ACG8E6_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFS5062368.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2093..3277
                     /gene="iadA"
                     /locus_tag="ACG8E6_001971"
     CDS             2093..3277
                     /gene="iadA"
                     /locus_tag="ACG8E6_001971"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFS5062369.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3388..3591
                     /locus_tag="ACG8E6_001972"
     CDS             3388..3591
                     /locus_tag="ACG8E6_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS5062370.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3692..4354)
                     /locus_tag="ACG8E6_001973"
     CDS             complement(3692..4354)
                     /locus_tag="ACG8E6_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5062371.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4367..5869)
                     /locus_tag="ACG8E6_001974"
     CDS             complement(4367..5869)
                     /locus_tag="ACG8E6_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5062372.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6417..8096
                     /locus_tag="ACG8E6_001975"
     CDS             6417..8096
                     /locus_tag="ACG8E6_001975"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS5062373.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8223..8702
                     /locus_tag="ACG8E6_001976"
     CDS             8223..8702
                     /locus_tag="ACG8E6_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFS5062374.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8921..9601
                     /locus_tag="ACG8E6_001977"
     CDS             8921..9601
                     /locus_tag="ACG8E6_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS5062375.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGDPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9609..10940
                     /locus_tag="ACG8E6_001978"
     CDS             9609..10940
                     /locus_tag="ACG8E6_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFS5062376.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10955..11917
                     /locus_tag="ACG8E6_001979"
     CDS             10955..11917
                     /locus_tag="ACG8E6_001979"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFS5062377.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12028..12366
                     /locus_tag="ACG8E6_001980"
     CDS             12028..12366
                     /locus_tag="ACG8E6_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFS5062378.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12445..13152
                     /locus_tag="ACG8E6_001981"
     CDS             12445..13152
                     /locus_tag="ACG8E6_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFS5062379.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13255..14736)
                     /locus_tag="ACG8E6_001982"
     CDS             complement(13255..14736)
                     /locus_tag="ACG8E6_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5062380.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4753 a         2557 c         3186 g         4430 t
ORIGIN      
        1 aaaacagaga aataaaatac ttaatataaa tggaaagaga aaaaaataat catacatagg
       61 agaaaaaaaa caaaatagaa aaaagtgatt ttctaataaa ttattcatgt ggaaatgtca
      121 tttttactgt ataattcatt tgattatcca ttttttattc atcaaaatta ctgaaaaaag
      181 aaaggaagaa ataaatttga agaaaacaaa ttcttcagga aaaccgaaac gcgacttcac
      241 taaattatcg acaccccata cgtatgtcat tatttttggt gtagtcatct ttgcatggat
      301 actgactttt gtcgttccag caggaaagtt cagcacgcaa gatattgaat acaaagatgc
      361 aaatggcgaa acaagtactc gtactgtttt acgtcaagat tccttccgtt atgcttacga
      421 gctggataag tcgtatgtgt ttgatcaatt agaagaactg caagatcatc cagcagaacg
      481 tgaaaaactg gatgttccgg aaaaaggatt ggaaaaagtc attgctgacg gagaaaaaaa
      541 tcttacgcag gaaaaactag atgaaatctc cttgacagat gatgtgcttt acgatcagta
      601 cggcgaaaat atctatgata cttctaaaaa attgcataaa acagccaaaa tatggggaac
      661 agatgatttt ggcggctttg gtttcttaaa ttttgttttt gaaggactag tatctggaga
      721 taagtatgga tctgctgtag ggatcgctgc attgatcctt gttgttggag gtgcttttgg
      781 tattattatg cgtaccggag cgatcgacgc aggtatctat gcgtttatca gtaaaactaa
      841 gggactggag cgtttagctt taccgctatt attctttgct ttttcattcg gtggcgctac
      901 attcgggatg gcagaagagg taattccatt ctcaatggtg atggttccat ttgtcattgc
      961 gttaggatat gactcgatcg tagcagtaac cgtgacttat gtcgcatctc aagtcggaaa
     1021 tgcgacctct tggatgagcc cgtttagcgt agcggtggct caagggattg ctggaatccc
     1081 cgtgttgtct ggtgcgacat tccgattgat tatgtgggta gttgttactg cattagccgc
     1141 tggttatatg atgatatatg cagaaaaaat ccgtaaaaaa ccagaaagtt cgttgactta
     1201 caaatcagat gattatttcc gttctcatat caaaaagaca tctgatgaaa ataaaaaatt
     1261 catgcttggg cataaattga tcttattaga gatgttagtt gtccttgtct ggatcgtttg
     1321 gggcgtgact caaaaaggct attacattcc agaaatcgct tctcaattct ttgtgatggg
     1381 attagttgct ggaatcattg ctgttctttt caaattagat ggaatgaaag tcaatgacat
     1441 cgcttcttct ttccaatctg gtgcagctga tttagctggt acagcaatcg tagtaggtat
     1501 ggccaaaggg attcttcttg tcttgggagg atctgatgca tctgttcctt cagctttgaa
     1561 tacgattttg catgggatcg ggacggcgtt gaccggtgtt ccagctgtca ttggtgcttg
     1621 ggcgatgtac atcttccaaa gtttgttcaa cttggttgtt acatcaaatt caggacaagc
     1681 agctttgact atgccaatca tggctccact ggcagattta gtaggtgtat ccagacaagt
     1741 agcagttctt gcttatcagc ttggttcagg ttttatggac gcttttacac ccgtatcagc
     1801 cagcttgatt ggtgtgctag gtgttgcacg gatcgaatgg gcaaaatggg caagattcca
     1861 aatcaagatg caaggattcc tgtttgtttt aggaactatt ttcatcatga ttgccattgc
     1921 aataggtctg cagtagtact ggagacctgt aaataaaaaa gtaatccaaa cagtagataa
     1981 ggactccaag aaccgtcatt ttacggtaaa atcaaaggag taaagatcct atcgtttgga
     2041 ttatttttta agatcctatc atcagttata cttaaagaaa gaggaaaaat gtatgaaaat
     2101 cattcgacaa attgaagtgt ttgcaccaga ttacttagga aaaatggatg tactgattgc
     2161 cggtgggaag attttggctg tagaagaaca gctagaaggc ggctacgaag gagtggaagt
     2221 agaagaactt tcaggtgagg gaaaagtact aaccccgggt ttcattgact gtcatttcca
     2281 tattttaggt ggaggaggag aaggcggtta tcaaaatcga acgccagaag tcacattgag
     2341 ccaattgact acagctggtg taacgacagt tgtcggttgt ttgggaacag acggagaagg
     2401 tagagatatg actgcgttga tcagcaaagc gaaaggtctg gaagcagaag gaatctccac
     2461 ttatgtatac gaaggttcgt accgattgcc ggtaaaaaca gtaacagatt ctatcatcaa
     2521 agactttttg acaattgata aaattatcgg aatcggagaa atcgcagtat ccgatcaccg
     2581 ttcttctcag ccaagttttg aagagttcgc tcgtgcagta gcagatgctc gtgtaggagg
     2641 catgttatcc ggaaaagctg ggatcatcaa tgtccactta ggcggaggaa aaagaaagct
     2701 agagctattg acacgaatcg tagaagaaac agaaattccg atcacacaat ttttgcctac
     2761 tcatgcaaat cgcacaccag aattatttga agcttgcgtg gcatttgcca aacgtggtgg
     2821 cacgattgac ttcactgcta gcgaagatcc tgatttttgg gagaagacag atggtgaagt
     2881 tcgcttcagc aaagcactga aacgattgat cgaggaagat gtttctttag ataatttcac
     2941 gatgacctca gatggacaag gaagcttgcc ttattttgat gaaaacaatc atttcctagg
     3001 gttaggagta ggaagtgcca aggcattgct agtagggatc aaagaagctg tgcaaaaaga
     3061 aagtatccct ttggaaatcg ccttacgagc tataaccagt aatccagcac gtatattgaa
     3121 attggataaa aaaggaaaaa tcgaaattgg tgctgatgca gatctgtgta tcttggataa
     3181 agaaacgctt gatattgata cagttatcgc taaaggagaa ataatggtcc aagaaaaaga
     3241 agtcaaagta tgggggactt tcgaaaaaag tttttaatca acagctctaa ctatcttttt
     3301 tagaaaacga gcgttctgtt tgtctgtatc tgttgttgat gttaccctga aggtagatca
     3361 ataaacaaac taagaggagg aagcacaatg gcagatttaa aaggacgttc taaagatatg
     3421 aaagataaag tagtcggtga aggaaaagaa gcatacggca aagcaacaga cgacaagagc
     3481 aaagaaacag aagggaaagc acaatcacta ggtgctgaca tcaaagaaaa agctcgaaat
     3541 ttaggcgacg aagtcaaaga caagttttct cgaaaagacg aaaaaaaata aaaaccactt
     3601 attactaatt gaatcaagca aataaaaaat ccgaaccata tctgcgagac agatatggtt
     3661 cggatttttt atttgatcat tatcctgttt tttaaacttc gattttttga aattttttat
     3721 tttttagttc aaagacttca tcacagcttt ttgctacttc aatctcatga gtcacgatga
     3781 taatacagcg attttgttca tgggcgattt cttggaaaag tttgacgata tcttgtgaag
     3841 ttgtttcatc tagatttcca gtaggctcat ccgctacgat tagttgatgt tcacagcaga
     3901 tagcacgaac gatagcgact cgctgctgct gacctcccga aagtttggtc acctgttttt
     3961 ttgctaatat ttcatcaatt cctacacgag ccagtcgatc taaagcaact tgtcgataat
     4021 tctcttgctt ggtcttagtg atttccattg ctgctatgac gttgtctaga gccgataaat
     4081 aaggaagcaa gttgtaagct tggaaaataa tcgagacatc atttttacga taacttctta
     4141 acccgatttt gttcagtgaa cgatcttgat agtatacctt tccttctttt ggtgcatcta
     4201 gtccagaaat aagcgaaaga aaagtcgttt ttccagaacc acttgctcct aaaatagcgt
     4261 acatttttcc agcttcgaaa ttcagttcta catctttata aagcgcatct tgcggatctt
     4321 gataccaaaa tcccaattgt tctgtccgta acatcaaatt cctcctttag tttaatagga
     4381 tttttcttgg gtttaatctt aatatcccta tagaagctaa aaagacagaa aacagactga
     4441 ttccaaatgc tactccggct aatagtccca attgtgcagg tttgacattg atttccaatt
     4501 cgctgacttg atcagaaaca gcaaatggat tattcgtggt tccttgttga ccatcgcgtt
     4561 gaccgccttg ttcttgtctg ttacctggca tttgtccacc gccaggagct tgctggtttt
     4621 gtgtcgtggt ttctgtctgt tggtcaagta gctgctgtcc cacggcattc gcgacgacgt
     4681 ttccgctaag cgaagcaact cctaatgcaa gaatcataca aatagccact tcggtaaaca
     4741 attggagaat gattttactt ctggattctc ctaaagatag caagacgcct aactcatgcc
     4801 gacgctcccg aatggtaatc ataatgatca gagtaagtat gataataccg gctacagcca
     4861 ctagtaaaac aacattttta gaaaaacttg ccacgttgtt taaaggttcc aacattgact
     4921 gatacatgga atcatttgat tgtaagctaa atgtatctgt gtcgatcagc ttctcagctt
     4981 ctttgagaaa ctcatccatt ttatctggat cagatagagt gtaaatagca gaatctatcg
     5041 tgttatcgtc agatgtccca tttagttcat ttgcaaaagt gtaagaagtg taaagtgtat
     5101 tcgacggatt catgaagttg aatttcatac ccatactgcc gcttgtttcg ctggattcat
     5161 aaattccttt gatcgtcatt tcataagtgg tatcttcatc ttcagtactc gtgattgtga
     5221 aagtatctcc gactgccagg tcatttgttt ctgctagagt agaatcaatg actacattgt
     5281 ttgtgccttt gtcgctgtca tctattcctt caccatccgt gatttttgct gttccatcag
     5341 aaaaattact attttgtgca gttgctgaca caccactgat ttgaaagtct gcttgagtca
     5401 tttgcggatt cccgcccatt cctccagcag gttcaccgcc tgcatcagta gtttctgaac
     5461 tgtcttcact tgaatcagaa ctagtgatgg cagttattcc atcttttgcc atagctgagg
     5521 cggaactttc aaaggaataa ctctttacat tatctaattc agctattttt tccgcgtccg
     5581 ttaaggaaac tggtgtaaga gaaaaactgc ctggatctgg acgttcttca gtggtagaac
     5641 cgcttgaaga agtttgttcc tgttttttaa atgaagcttc tcgattggca cttaaagtca
     5701 ctgttgctcc aacttctttt tgtgcttgat tcgctgcttc ttctgcagcg cttcgaatcg
     5761 tgaggccagc tagcacaaaa ataagaatgg ctgtgaacac ggcaaacaat aacaatgaac
     5821 gtccccattt tacttttgta cttttccatg cacgtttcat aaaattcata ttttgcatcc
     5881 ctcctagtag atgcatttga taatagacaa cctatcttaa agctagctta aatcttatgc
     5941 tgtttgcaca ccatcaacat gaggttctgt atatataaaa agtcactata gttattgcaa
     6001 aaaatgtggt aaaatgaaaa aagtatgttt caaaccgacg aagaagaaat gaaagaggaa
     6061 tagcaaaaga aatcctagat gctacagagg gatttttacg tgcatagttg ttctaacaat
     6121 tgctgatact agcgatttag tttatttgtt gtataataat ggggaacagc tgaatactta
     6181 tataagtatt ctggctatag cgtttcgcaa aagatccatt ttgttaatga agtatatcca
     6241 aaataaaatg cccatccaac tgtcagaacg cggatgtgct ggtaattttc ctgctgattc
     6301 tccataagtt gagaaatagt gattttgcat atgcttcatt ggaatggctc atatatttag
     6361 ataaagaaga aaatcgacat ctttaaactt ggatgtcata agaagaggtg aagtttgtga
     6421 gtaccattaa aattatccca ttaggcggcg ttcgtgaaaa tggaaaaaac atgtatgtcg
     6481 cagaagtggg cgaggatatt tttattttag attgtgggct aaaatatcct gaaaatgagt
     6541 tgttggggat cgacgtcgta atccctgatt ttacgtattt agaagaaaac attgatcgtg
     6601 tagctggtgt ctttttaaca catggacatg cagatgcgat cggggcactt ccttacttgt
     6661 tgtcaaaaat ttctgtacca gtattcggga cagagttgac gatcgagtta gcaaaattga
     6721 atgtcagtcg taatgaagca gccaaaggat ttaaggattt ccatgtggta gatgagcata
     6781 cagaaataga ctttggtgaa acagtagtga gctttttccg taccactcat acgataccag
     6841 attcagtggg gatcaatctg aaaacaaaag aaggtagtat cgtttatacg ggagatttta
     6901 aatttgacca aacggctatc ccaatgtatc aaacagatta tgcacgattg gcggaaatcg
     6961 gcaaagaagg agtcttagcc ttactaagtg aatcggcaaa tgcagaaaat cctcagcctg
     7021 tagcttctga agcccaaata gcagatgaag tatttgatac gatcaaatat tgggaagggc
     7081 gtatcatcgt cgcttgtgta gcaagtaatt tgcaacgagt gcaacaagta ttggatgcag
     7141 cagaaaaagc acaacgaaaa gttgtcttga ctggtcaaga tttcgaacga attattcgca
     7201 cagcgatgcg tttagaaaaa cttcaattac caagtgaaga tctgcttgtt acgttgaaag
     7261 acatgaaaaa ctatgatcca gaagaactga tcattttaga aactggccga atgggtgaac
     7321 ccatcaagtc tctgcaaaaa atggcaaacg gtacacaccg taatgtcaag attgaagaag
     7381 gagatttggt ttatatcact actacgccaa gcatcgcgat ggaaaccatc gtggcgaaaa
     7441 cggaagatat catttatcga gctggtggaa cagtgaagtt gatctctgag aatatgcgcg
     7501 tttctggcca tgccaatcct aatgacttgc aattaatgat caatttgatg aaaccaacat
     7561 attttgtgcc agttcaagga gagtatcgtg aattagctgc tcatgcagat ttggctcatg
     7621 cagtagggat gccatacaaa aatatctata ttactggacg cggagacgtg ctggaatata
     7681 agaacaagcg tatgagtgtt gcaggaacta caagtgcaga aaatgtaatg attgatggat
     7741 taggcatagg tgatatcgga aacattgttt tacgtgatcg aaaaatcttg tcagaagacg
     7801 ggattttcgt agcggttgtc actatcagtc ggcgagaaaa gaaaatcatt tcaaaacctc
     7861 aaattacttc acggggattc gtttacgtaa aagctagccg tgatctgatg aaagaaagca
     7921 gcaatatgat tgaaacaatc gttgagaaac atttagaaag caatgatttt gagtggagca
     7981 aattaaagca agacatccga gatcaattag gccgttactt atttgaacaa acaaaacgcc
     8041 gcccagtgat cttgccagtc attatggaag ctactcaacg tcgaaataaa aaataaacaa
     8101 aaagagtaag atgaacctct ctatagagtg ctcgtcttgc tctttttttg cgaaaaacaa
     8161 ataatttttg aatacagcat ccctcgcttt ataataaacg atagaataaa gggagtgaat
     8221 caatgatgag tcaaacaaaa gtcgaacgtt atttagaaga acaaaagatc agctatcaac
     8281 cacttgattt tagtcatgtg gtagaagaat ctttcacaga agaactacaa aaaagaggga
     8341 ttgatccagc tttgatatgt aaaactctcg tagtgaaagg aaacaaaact ggggtgatca
     8401 tcgcattgat tcctttgaac gatcacttag attacaaaaa aacgagacaa gtgacgaaag
     8461 atcgtaaagt tggttttcct ggaatggatt ttgtattaat gcatacagga tatccccacg
     8521 gagcaaacac ccctattgga ataaaactcg cacatcctga ttattgtttt ttagcagaca
     8581 gttctttaaa agaaaaagaa gaagtaattg tgtcaagcgg tgaaatcggg agaagccttc
     8641 ggataaaagt agccgattta gaaaagatcg tccatcctat ttacgctgat ttaattagtt
     8701 aagggacgag aagagaagct aagcagagca gagacctcaa acttgagatt atatctgctt
     8761 ggtacttggt gcatcctgta ttatctgtta caattgcttt gtacatactg tactatcaat
     8821 ccaatgaaaa tatatttagc agctatattt tttcattgat gtgcaagtga tgaaatcgtt
     8881 tttaataaaa aagatacaga gaaaacgcga ggtgtaaaat atgaaaatta cgtatcatgg
     8941 acattcctgt ttaacaattg aaatggaaaa tggccaacgc gtgatgattg acccgtttat
     9001 tacaggaaat ccattgacag acttaactgc agaagaagta aagacagatt ggttgctgat
     9061 tactcatggt catagtgacc acataggaga tatgattcct attgcaaaag aaaatcaagc
     9121 cacaattatc agtattgttg agattgctga ttatgcgaac agcagaggag tagattcatt
     9181 cggtatgaat atcggtggaa aacatgcgtt tccattcggc gtggtaaaat tcgtacatgc
     9241 acagcatagt tcaagctatg aagtagacgg tatcgttcag tatatgggcg atcctagcgg
     9301 aattattatt caagcagaag gaaaaacgat ttatcatgca ggggatactg cttatttcag
     9361 tgatctagga ttgcttgcag aagaattcga tattgatgtc gctttcttgc cgattggcga
     9421 taactatacg atggggccag aagatgcagc aaaggctgcg caaacaattc gtgccaagaa
     9481 agtagttcca atccattaca atactttccc tgtcattgaa caagatcccg aatcttttgt
     9541 agagttactg gaagatagtg aaggaaaaat aatgagcgtc tcggaaacgc tggaagtgta
     9601 ggcagaaaat ggcgacaaaa catgatttga ttttagaata tattgaaagt ttacctgttg
     9661 gcaatcgaat ttctgtaaga agcatcgcaa aagaattgaa tgtcagtgaa ggaacagcgt
     9721 accgtgcaat caaagatgca gaaaatgtcg gactagtttc aacaattcaa cgagtaggga
     9781 ccatccgtat cgaacgcaaa ttaaaaaaac atattgaacg tttgacattt ggcgaggtcg
     9841 tcaggatcat tgaaggggac gttttaggcg gggcggcagg attagacaag gtcttgaata
     9901 aattcgtcat cggcgcgatg actaaacatg ccatggaacg ctatattacg ccaggatcac
     9961 taatgatcgt cggcaaccgg gcggaagtcc aaaaattagc attggaagat ggcgccgcag
    10021 tattgatcac aggaggattc aatacgactg ctgagattgc aaagctagct gatgatcttg
    10081 aaatgccgat cttacgcacc tcttatgata catttacagt cgcaaccatg atcaaccgag
    10141 cgttaagcga ccaattgatc aaaaaagata tcatgcttgt cagtgatatc tatatgccag
    10201 ctgaaaagac tcattatttg cagcagacag atacgatttc tgattatcag cgtttgtcag
    10261 aagaaacgca gcattcgcgt tttccagtcg tcaaccgcca tcatcgtttg atggggattg
    10321 ttacggcaaa agatgtttta gggaaatcgc caaaccagat catcgatcgg gtgatgacga
    10381 aagaaccaat cagtgtaaag aaaacaatga gtattgcctc tgttagccat caaatgatct
    10441 gggatgggct agaagtcatg ccggtcgttt ccgatgactt gacattggaa ggcttgatta
    10501 cccggcaaga tgtaatgaaa gcgatgcagc ttgtccagcg acaaccgcag attgcagata
    10561 cgatttcgga tcagatatct ggaaatatcc atacgattga tactgaccgt gaaggaaatc
    10621 gtttggaaaa ccctaagttc aaattcgtag tagcaccaca aatggtcaat ggcgttggaa
    10681 ccatttcttt tggcgtactt agcgaaatca tttctgatgt cgcacaaaaa acaatggtga
    10741 tgaaccaaaa acgaaatatc ttgattgaac aagtgaacct acattatttg cggctgatcc
    10801 aattggaaag cgaattagac attcgtccac gtgtattgga aatcggcaga cgttccgcta
    10861 aactggatat cgaagtgttt attgaaaaca ccatcgtttc caaagcaatc gttgtttgtc
    10921 aagtaatgga acgttcttag aaagcaggag tgaaatgaca gtacagcaag aaatagtaga
    10981 tacaataaaa aaatacgata aaatcattat ccacaggcat ttgcgccctg atcctgatgc
    11041 attaggatca caagtaggat tagccgaact attacgggaa agtttcccag aaaaagaaat
    11101 acgtcaagta ggtgaaacga ttgaagggct tcgcttttta gcagaaatgc aagaagttga
    11161 agatgtcttc taccaaggag cgctagtcat cgtgactgat acagctaatt cacccagaat
    11221 cagtgatcaa agatatcagt tggcagaaaa actgatcaaa atcgaccatc atcccaatga
    11281 tgacccttat ggagatcttg tgtgggtaaa tacaaaagca agtagctgta gtgagatgat
    11341 tgcagaattt gctttgatgt ttcctgaatc tttaaagatg aacgttaagg ctgcacggtt
    11401 actttatgca ggaatcgtcg gagatacagg gcgctttttg tatccctcca cgacgtcccg
    11461 tacattagag attgcttcga tattgagaaa ttatccattt gatgcaagtg ctttgaacag
    11521 agagattgaa caaatgccga tgaaagtcgc taaactttcc ggttatcttt atcaaaatat
    11581 ccaggtggat gaaaatggtg caggaagaat aatccttccg caagaaattc ttgataaata
    11641 tggaatcgat gattcagaga cagcttcgat cgtttcactt cctggagtga ttgacgaagt
    11701 gctggcttgg gggatttttg ttcagcagcc ttcagggtat taccgcgtac gtttacgttc
    11761 taaggggccg atcatcaatg aattggctaa acgccatcat ggaggaggcc atccattagc
    11821 aagtggtgcg aatgcaaaag atttagcaga agtagaggaa atctataaag agatccaaga
    11881 tatatgccaa tcgtataaga gcagaaaaac agaatgatac ttcgttcgaa aacgtgcctt
    11941 ttgtagtaaa aatcacaaat gtatcttttc aagatcttct ttttccgtta caatagtgct
    12001 cactaaagat tttaaaggag aaagaagatg acattaccaa attgcccaga atgtggctca
    12061 gagtacacat atgaagatcg aggcttgttg atttgcccag aatgcgggaa cgaatggaac
    12121 aaagaagaac aagaaactgt catagaagac ggattagtgg tattagatgc aaatggaaac
    12181 cgtctgttag agggcgatag cgtgacagtg attaaagact tgaaagtaaa aggtgcgtca
    12241 ggtgccatta aacaaggaac aaaagtcaag aacatccgtt tagtagaagg cgatcataat
    12301 atcgattgca aggtagaagg atttggaccg atgaaactga aatcggaatt tgtgaaaaag
    12361 aattaaagaa tattcaaggt cttttcacat tcttttccta ctactaatga taaaataaag
    12421 taaacgaaaa ggagtgaaac gaaaatgtat ggttatggaa tgtatggttt tttagatccg
    12481 acatttcttt tggtcattct tggattagtt atttccggaa ttgcgtcagc gtacgtcaat
    12541 agtactttcc gaaaatacga tcaagtgaga agcaaaaaca atgtgactgg tacacaagca
    12601 gcacaatata tattacaaag tcaacagata aataatgtcg gtgttcaaca aattgccgga
    12661 gatctcacag ataactacaa ttcaggtaat aagatgttga gtttgtctca agcaactgca
    12721 cagtctactt cagttgctgc gataggtgta gcagctcatg aatgcggaca tgcggtacaa
    12781 gatgcttcgg gttatgtccc attgaagatt cgtgcagcac ttgttccagc agctaacttt
    12841 ggttcgatga tttcgttccc cttgattatg gtaggtgtat tgcttagctg gaactctacg
    12901 ttaatcaata tcgggatttt cgctttttct ttagcattac ttttccaatt ggtgacatta
    12961 ccagtcgaat ttaacgcgtc tcggcgagca atccaaattt tgagtgaagg cggactactg
    13021 acggaagaag aagtaccgat ggcaagacac gtattatttg cagcagcact gacttatgtt
    13081 gcagcagcgc tttcgacatt cctgcagctg ttgcgcttga tcttattgtt cggcggcaac
    13141 cgaagagatt aaaataaaga agttgtgaaa tcagcgcttt gatctgagct aataccgaga
    13201 gttagctgat tgaataccat ttatttggaa taagaggatg tgacaagaat tttgtcacat
    13261 cctctttgtt ttgttctcat aacctttttt catatacagt tcatgaaccg cttttattaa
    13321 cttgccaaat aacactgtat tgtgcgttgt atccagatag aaagtctccg tttcatctga
    13381 aattttctca tgctgatctg taatgatcaa atcagcatgt tctgaatatg cggtaatttt
    13441 aacagcatct ttgcgaaata actgctgcaa atggctttga agatgataat ttcctgtaaa
    13501 ctgtttggtt atctgcaaat ggatgagtaa ctgctctttc gtttctgttt gtagtaagtg
    13561 ttggatcaat tgactaaggt attgactgat ttggtttctt tccccatttt ttgaaaaaat
    13621 tgtatcgatt ttttctctta atttcttttc ctgtataaaa gaactagaat gattggacgt
    13681 ggcttctgga aagcaaagtg tagaaaaact actgctggat tcacccaaca gttcgaaaaa
    13741 aacaactgcc aacacaagaa aataaaaata aattggtatt ttattttcta aatggttgag
    13801 aggaaactgt tgagttaagt aagtcatcaa ttgaccgact tgtcgacaaa taaggttttt
    13861 gcttgtggaa aataaaaaac caatctcctg tttttgtcga aaagagattt gatttggaaa
    13921 aaaacaatag aaaaaataat taaagtaggc tctctctgtt tctgtctctt cacaagggcg
    13981 actcactgaa aaagaagtaa agtattctga ataatcatgt gtatgctgca ataagtccat
    14041 caattcagtg atttcagaag aagtttttgg aaaatatgcc tgttgtttgt ttcgtaatat
    14101 gaataaagtt aacagcaagt agatattttt ttcttgtttt gaaatttttt ctatttgctc
    14161 atctgtactg ttcattttta gtgcatttaa ttcaagtgag gaaaatggcc aatcgttacc
    14221 tccaaatgta tctgaaagaa aaaagtaaag atagagacgg atagataact catttccttc
    14281 aagagtacag caatttttta cagataggat ccgtaaatgg aattttttta aggaatgatt
    14341 gagctgtttt gtcagttttt cgagatagga gggcgatata taaagttgtt caagtaaata
    14401 tttttttggt acagcagttt gtggataatt gattagtaac atcaaatagt taaaacgatt
    14461 ggattctttt aggtagcata attttaactt agaaaaaaca gctaactttt tagtttgatc
    14521 ggtttctttg gtttggatct ctcctttatg attagaataa ataaattcat caatcaataa
    14581 aaattgcttg agtgatacat ttatttttcg gatcagttct ttgattgtcc gttttggaat
    14641 atgtaagtca ttggataatt cttgtaaaga acaattattt ttttgaatga ccatttgtag
    14701 aacaagtaac tctttttttc ttgcttcaat tagcataaat acctcctttt tattctgttt
    14761 ttgtatatag ttagacaaaa actgtgagtg gaaagtcatt tcagtctgtg ttattctcac
    14821 agagtgtcgc cttctttttt atataacgtt atttaatttt tttgttacct tgtttattct
    14881 attgtttatt tcctagggta ttttgtcaag atattcttgt ttttaa
//
LOCUS       DAZUMM010000064        14818 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000064 DAZUMM010000000
VERSION     DAZUMM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.064"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACG8E6_001983"
     CDS             complement(296..1024)
                     /locus_tag="ACG8E6_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5062381.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACG8E6_001984"
     CDS             complement(1002..2576)
                     /locus_tag="ACG8E6_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS5062382.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACG8E6_001985"
     CDS             2805..3743
                     /locus_tag="ACG8E6_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5062383.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACG8E6_001986"
     CDS             3755..4666
                     /locus_tag="ACG8E6_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5062384.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACG8E6_001987"
     CDS             4690..6162
                     /locus_tag="ACG8E6_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5062385.1"
                     /translation="MKKNMQKYLIGGVSVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6214..6825
                     /locus_tag="ACG8E6_001988"
     CDS             6214..6825
                     /locus_tag="ACG8E6_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5062386.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6855..8768
                     /locus_tag="ACG8E6_001989"
     CDS             6855..8768
                     /locus_tag="ACG8E6_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFS5062387.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8896..11877
                     /locus_tag="ACG8E6_001990"
     CDS             8896..11877
                     /locus_tag="ACG8E6_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFS5062388.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11874..13535
                     /locus_tag="ACG8E6_001991"
     CDS             11874..13535
                     /locus_tag="ACG8E6_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFS5062389.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13575..14507
                     /locus_tag="ACG8E6_001992"
     CDS             13575..14507
                     /locus_tag="ACG8E6_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFS5062390.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4817 a         2334 c         2977 g         4690 t
ORIGIN      
        1 atttaaataa ctacatccat ctattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt acaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgtttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgacga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttta ctaaatgaaa
     6181 agaatagact aagcagttag tggggggatt caaatgaacg aaaacaaatg gaccaaagga
     6241 tttaattggg tttttcaatt gttattacta aactttactt attttatact aatttgttta
     6301 ggatttggaa ttttaggttt tttccctgct acgatagctt tgattgctgg cgttgtgaat
     6361 cgaaaaaaat atggcttgca agagtctgtg aagttcatga gtaaaatcta taagaaaaat
     6421 tttgttagat ataatctata ttgctggtca gcaactttgt tgttggccgg caatattact
     6481 acgatctggc tttgttggca gcaaagcgca ccattgtttt atttattaag tacattatta
     6541 tcggtaatta cactgttcct agtctttgca atgttgaatt gggtcgtgat caaaagtctt
     6601 gggttgtgga tgatgggaga ccgactgatt gactatttgc ttttcacagc aacgatgttg
     6661 ccaacggtag tcatgcaagg cttggtgctt ggaatttatt tccgaatatt gatgattttg
     6721 cctgtgattt ttctgtttgg cggaacagct gtaacggtaa tattgattag taaattaact
     6781 gaaagagcgt tagcaaagtt gccttgtcag atacaatcgc actaattttt tcacaaaaag
     6841 gagtatttct ttctatgaag ttaactgata aacaagtagc taatatagcc tttatccagc
     6901 aacttgagat tccattgaca caaaaagatg aagaacaaat agacttgatt tttactgaca
     6961 caaaggctaa tgtgcgacaa atcaagatta ctaaaaatga gcagcaacga atctgcttgt
     7021 cttattcaca tgaagacttg ctgtatcgag gattgtttat ctgccttcca cgttttttag
     7081 ctcaaggagg agagatgcca tacattgaag aaacctcgat cattgagacc agttattcat
     7141 tagatgcttc tcgtaatggc gtgatgaata aagagactct aaaaaaattc atttcttact
     7201 tagcgttgat ggggtacaac acattgtata tttacacaga agatacctat gaagtagaag
     7261 accttccata ttttggttac cttcgcggag cgtatacaaa agaagaagtg aatgaaattg
     7321 ttgaatttgc ttctggtttt ggcatagaag tagttccatc gatccagacc cttgctcact
     7381 tgacccaatt tttgaagtgg tatgctcaac aagaactgat ggatgatcaa aatactctac
     7441 tgatcggtga agaaaaggtt tatgcggcaa tcgaaaagat gattattgcc tgcaaagaaa
     7501 tgtatcagac agagcgtatt catttgggaa tggatgaagc ctatcaagca ggtctgggac
     7561 gatatttaag cctgtatggg tattgtgatc ggacaacttt aatgattcaa catatggacc
     7621 gtgtgttatc aattgttgat aaatataaga tgaaaccaat gatttggagt gatttcattt
     7681 ataagctatt agatgaaacc ggtaaagata ggtattacaa tcctgatttt aaactcaaag
     7741 cattagcagc taaaggatta ccagcaggat taacctatgt tcattgggac tatggttcag
     7801 atgaagtttc ctcctatgag aaagtaattg ataaccattt gaaattttgt gacattgatc
     7861 attacgtttt agctagtggt gcccatatct gggggagact agccccgaat catgggaaaa
     7921 cagaagcaat tgtttctgca gctgtaaaag cttctaaaaa taaaggtgta cgttctgtga
     7981 tgttaactac ttggggagat gaagggcagg aaacagagca ttggcattcc ttaattgggg
     8041 cattaaaatt agcagattat gtctattctg aagaagagtg gaatgatttt gaggaaatct
     8101 acgatcgttt attaggaaca ggtagttatc aattaatgtc cgaattacga tatttcgatg
     8161 aagtggatac cattacaaat aacaacttac aaatggcttc cgtgagtaaa ctgctacttt
     8221 ggcaagatcc gttagttggt atatatgact ttcatgtagc cgctcataac aaaattgcca
     8281 ctagaacaat tggtacttat tatcatgagt tagctcagaa attagatatg aataaaaatt
     8341 ggaatccaaa acaaccaatt ttaacaacta ttcgtcaacg atatatactt ttagctgaaa
     8401 ttttagcaca aaaggcagaa ctaggcatct atttacaaca ctgctgtctg gtagaagacc
     8461 ttgcaggatt aatggaatta gcgaatcggt gtgaagcact agccaatcga gtaaatgaat
     8521 tggcatctat tcatgaaata atttggtatc aaacatacaa aacctatggt tgggaagtat
     8581 tagaacaacg atatgctgca ttagctcatc gtctgaaaac cgttgcgcgt cgtattaaaa
     8641 atcatcttca ggggcaagat gccctagagg agcttttgga agaacgatta ccgttttgtc
     8701 aatatcaagt gccgcttgaa atttctggct ttaattatcg gcaaagctcg gtgtcaggct
     8761 acaattaacg aaggaatctt cccgttgaac ggaccacttg ctcatctaat gggaaagtgg
     8821 ttcgttttct attcataagt agcttaccac taactctttt attaagaaga gaaaaaaagt
     8881 aggaagtgag aaaatatgtt atcggactat tatcaaaatc tgtcagttat aagtgtcaat
     8941 acagaaccat tacgtaatta ttatattcct tatgcaagta aagaagaagc aaaacaagag
     9001 ttagaaaggg aaaaaacgag ccagtttatc gatctcaatg gtacatggga ttttcagtat
     9061 tatccttctg tccaacagtt accggaaaat ttttttcaag aggcctttac tgaaagttat
     9121 gatagaattt ctgttcctgg agtttggcaa acagagggat ttgacttacc tcagtatatc
     9181 aatgtatctt atccaattcc ttttgatccg ccttatgtac ctaatgatac cccttgtggt
     9241 gtatatcggc gtcagtttca aattgagcaa acagatgcac gcttttactt aaatctagaa
     9301 ggagcagata gtaattgttt tgtttggata aatagaaact ttgtaggcta tgaccaagtg
     9361 agtcatagta catctgaatt tgatgtgacc aagtatttac aggttggggt taatgaaatt
     9421 caaattctag tgatgaagtg gtgtgatggt acctactttg aagatcagga taagtggcga
     9481 atgagcggtc tatttcgtcc agtgtatctt cttcaacgac caactatgca tctgacaagt
     9541 tatcaactac ggacgttgac tgatcctaaa aaaggaagcg gaacattaat ggtttctttt
     9601 caacgattta ctcctattga agtatcgtta cctatttctt acgaattatt ccatcctgat
     9661 ggacgtctca tagcgaaagg ggagtgttac tcggaacaac aaataaattt ggatgttgaa
     9721 gattgccttt tttggacagc agaaacgcct aaattatata cattactttt aacatgtaat
     9781 ggtgaatatt ttaaagaaaa agttggcttt cgagcactga cgatcaagaa tcgtaagtta
     9841 ttgctgaacg gaacacctat taaattatat ggagtcaact atcatgacgt gaatcagcaa
     9901 ggcacgccag tgatgacccg agagcagtta cgtgcagatt tgttattaat gaaaaaatta
     9961 aatgtgaata ctgtacgaac ggcacattac cctaaatcac cagattttta tgaattggcg
    10021 gatgagattg gtctgtatgt aatcaatgaa gcagatttgg agtgtcatgg tgtggttaat
    10081 ctatatgata ctgaagcgaa ttatcaacta attgctaatg atccgacatt cgaaaagccg
    10141 attatggacc gagtgatccg ttctattctt ccttatcaaa atcgaagctc tatcattatg
    10201 tggtctatgg ggaatgaatc tggttatgga gtaaattttg aaaaagctca agctttagca
    10261 cggaaattgg atgattcacg gttgattcat aatgaacgct cgattgaacc agttattgac
    10321 caagtgaatg attttagtaa cttggatgtt attagtcaaa tgtatccccc actggaagat
    10381 gttcatcgtc attgtcaaag aagtgatact gaaaaacctt tttttatgtg tgaatatagc
    10441 cacgctatgg gcaacggtcc tggagatctt aaaacgtact tcgatttgat tcaacagtat
    10501 gattcctttc ttggcggttg tgtctgggag tggactgatc atgcaatcaa tagaggaact
    10561 ttagaaacgc catcttattt atacggtggt gattttggtg aaaaatatca cgacggcaat
    10621 ttttgcgtag atggtttatt gaccccagat cggcaattaa cagtaggtgc attggagtac
    10681 cgtaatattc attcgcctat tcaactgatt gctgtagatg aggagctgca agaagtaacc
    10741 ttgagcaata taactgcttt tacagccggt tcagcattta acctttctct tgaatggtat
    10801 atagatggaa aaaaaacagt ttatgaagta atgaatcctt tcatacttga gcctggcaaa
    10861 agttgttcat tgcccatcac agtacctaca acgattcatg gtgctcatac ggttgctgtt
    10921 tcgctatctg tttataaaaa tgatgagctt tatggacagc aacagctctt tcttaaaaaa
    10981 gaaaaaatga gattgccaag aactgctcat cataatgatg caccacaaat ttcagtaaaa
    11041 ggcacagagc tgataatcaa aggagaatcc tttatttata tatatcaatt agcaaaagct
    11101 gcgttcactt ctgtaaagat caatcaaaag gaacttttgg aacgtcctac tcaattttca
    11161 atttggcgtg caccaacgga taatgatcgt aacttagtaa acaaatggaa agcagctgga
    11221 tatgctgaaa tgtctttaga aatcagaaaa aaagactgga aattcgtaga tgatcatctc
    11281 ctgatcgaaa cgtcttatcg aggagttatt ccaggattac agccaatttt tacaatgcac
    11341 atacagttga aaataaatgg tgcaggcgta atcaaccaaa aagtatcagt ggagaaaaat
    11401 ccgatttttc cagatttccc tcgctttggg atggactggc cgttgaatga tactttttcg
    11461 aaggtttctt attgtggcta tgggccgtat gaaagttatc ctgataagca ctatgctgcg
    11521 atgtttggta tttatgagat ggagattgag gaatttgatc agccatatat caaaccacaa
    11581 gaatatggta accgctggca atgtgattgg gtatcaatca aaaatcatca aggatctacc
    11641 atgacggttt atggaaactc taatttttct gctctgcctt atagtcaagc tcaattaacc
    11701 gaaaagtcac accattatga attaataaaa gaaaatagaa cttggttcaa tttaagttat
    11761 aaacaaaatg gtatcggctc acacagttgc ggtccttatt tagaagatcg tttccgtttt
    11821 atggaagata cttttgactg gaatatagtg attactttca aaggagaaaa tagatgaaag
    11881 aaacaaagat caatcaagtc aaaaaaaaat tatctcgtta tcaagaagtt gtttatcgaa
    11941 accattgctt aaaatggtca aaaaataagc tgaatgttcg ttttaatgga ttattttatc
    12001 gtttcgaaca gttagaagaa gtctggaaac cggtaacgaa tttccaatca tgtcctgtac
    12061 agctcataaa tgatccaaaa gaaacatacg atttatgtat aacacacctt tataaccagc
    12121 atttgaaaaa tgacggattt tatttaaagg ttactaatga caatcaactt acgattgaat
    12181 cagcaaacct tcgagggttt caatatgcta tggaagcctt attagagagt tttttttcgg
    12241 taggagatca attattatta ccggaattga ttctgaaaca tgaaccatca gtgaaaattc
    12301 gaggaatcat tgaagggttt tatggtatcc cctggacaca tgaaatgcgc ctctctcttt
    12361 tttcatatat gaaagataac caattgaata cttttatgta tgcccctaaa gatgatgaat
    12421 tattacgtaa aaaatggcgt gaactctacg atgcgcaaga attagacaaa tttaaggagc
    12481 ttttatctgc tgctgaggct agcaatatcg atttttggta cttaattagt cctggaaatg
    12541 atattgatat tacgtgtgaa gaagacattc aagttcttct gaaaaagtta gaacaactga
    12601 ttgagcttgg cgtatttcaa tttggtctat taatggatga tattgactat caattaaaag
    12661 gtgctgctaa acgccgtttt cgagagccag cattcgctca tgcctattta gtgaatcgag
    12721 tagaggaata tcttagcact cggttgtcca atcatgaatt agtcatttgt cctacagaat
    12781 atgataatcg tcatggttct cgctatttag caacgttaag tcaagaaata ccagaacaat
    12841 taccgttctt ttggactggc ccaagcacgt tagcggcgtc aatttctaca gaagagctgc
    12901 aggaaatggc ttctgtctat caacggccga tgctgatttg ggataatatt ccggtaaatg
    12961 attatttaga ggataaagaa ctattgttca tgagtccata tgagaatcga acaccgaatc
    13021 tttctaagga gagataccag gttacaggag tagtttcgaa tccaatggct caattagaag
    13081 cttcaaagtt tacgatcaat agtatggcta attacttatg gaactgtgag cgttttgatc
    13141 ctttggaaac ttggacgtca gtcgttgaaa aagtagttgg aagtaaacta caaccagctt
    13201 atcttacctt aaccaatgct tttccaaatc attacacaag cagtctgctt tcagatgaag
    13261 aattactgct agccaaagac ccagaatggg tcacaaaaga aatggctgct ttagtccaag
    13321 cagtaaaatg gatccagcaa gaacaacata catctagatt gagaacagaa ttggagcctt
    13381 ggttacaagc aatcacagaa agttggactt tctggcaaga atggcaatta aaaaaagatc
    13441 caaaagaagc agaagaatgg ttggctaaga aaaaaacaca ccgcatagga cgtgatttag
    13501 taactgccca tttagagcaa attatcaaaa gttagatatc ttaaaaaata aaaagcaaga
    13561 aaaaggagaa caaaatggtc aagagattaa ttagtgcaaa ctacagtgat gtcaagaaaa
    13621 tgacgtcaaa agagttgaag caatccatca aagcgagtga aggacgggtg attgtttgtg
    13681 aaacggttgc taatactctt ccacaatctg gagaaattac gaatgcagaa gttgcgtcag
    13741 catttggtgc agatttgatt cttttaaatg gtttcgattg ttatcgtcct gcagtttttg
    13801 gactaccaaa tgtgacgatt gagaaggttt ttgatctaca gcaaacgatt gaaaacccga
    13861 ttcctaaatt aaaagaacta gtcggtcgtc ctgtgggaat taatttagaa cccattgccc
    13921 ctaaaacaaa tatgatgagt gagaaagtag aaatcagcca aggaagagca agctctgttg
    13981 aaacgataca aaaagcagaa gaactaggag tcgattttat ttgctttacc ggtaatccag
    14041 gcactggagt aaccaattta gaaattacta aatccgtaaa aatggcaaaa aagtattttt
    14101 cgggattgat tattgctggg aaaatgcata gcgccggctc agaagagcca gttgtgaata
    14161 aagaggcgat tatttcttat gcggaagcag gcgcagatat tttactagtt ccttcagtag
    14221 ggtctatccc aggcttttta gagcaagacc taattgaagc ggttcggttg gcaaaaaaat
    14281 atgatttgtt aacgatgtct gcaatcggta ctagtcagga aagtgcccga ccagaaacat
    14341 tgcggcaatt agccattatg aataaaagtt gtggtgttga tttacaacat attggagatg
    14401 ctggatacgg gggattggca ccagcagaaa atattttcga aacctcggtt gcaattagag
    14461 gaatgcgaca taccatcaac tgcgttagcc gctcgatcaa tcgctaacaa aattaaataa
    14521 aaaatggtta aattgattat ttaagtggga acatacttga gatatcagct aacttctgtc
    14581 actttaaaaa agggactgtg acagaagtga aaatagacaa aaaatatccg aacaagagac
    14641 taggatagca gaaaaactat cgttttgcaa cgaggaccaa ctggaccgta ggagcaattc
    14701 tcttattgtt cggatatttc gcttgttcaa ggacttatgt ctgtagtgcc cttttttggt
    14761 aggaaaaatg gtagggaacc atatcatttt taatgaaaat caagcatttc aaaagttg
//
LOCUS       DAZUMM010000065        14655 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000065 DAZUMM010000000
VERSION     DAZUMM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.065"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..1051
                     /locus_tag="ACG8E6_001993"
     CDS             155..1051
                     /locus_tag="ACG8E6_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS5062391.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFNMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLAYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPIPSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1201..2142)
                     /locus_tag="ACG8E6_001994"
     CDS             complement(1201..2142)
                     /locus_tag="ACG8E6_001994"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS5062392.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFRSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKTAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2267..3388
                     /locus_tag="ACG8E6_001995"
     CDS             2267..3388
                     /locus_tag="ACG8E6_001995"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS5062393.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPHGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3646..5148
                     /gene="eat(A)"
                     /locus_tag="ACG8E6_001996"
     CDS             3646..5148
                     /gene="eat(A)"
                     /locus_tag="ACG8E6_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS5062394.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKSQPKHLSISYVRQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5215..5742
                     /locus_tag="ACG8E6_001997"
     CDS             5215..5742
                     /locus_tag="ACG8E6_001997"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5062395.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5953..7014
                     /locus_tag="ACG8E6_001998"
     CDS             5953..7014
                     /locus_tag="ACG8E6_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS5062396.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7017..7913
                     /gene="murQ"
                     /locus_tag="ACG8E6_001999"
     CDS             7017..7913
                     /gene="murQ"
                     /locus_tag="ACG8E6_001999"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS5062397.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7927..9909
                     /locus_tag="ACG8E6_002000"
     CDS             7927..9909
                     /locus_tag="ACG8E6_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS5062398.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSAATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9974..10420)
                     /gene="nrdI"
                     /locus_tag="ACG8E6_002001"
     CDS             complement(9974..10420)
                     /gene="nrdI"
                     /locus_tag="ACG8E6_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS5062399.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10631..12055
                     /locus_tag="ACG8E6_002002"
     CDS             10631..12055
                     /locus_tag="ACG8E6_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFS5062400.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12236..13009)
                     /locus_tag="ACG8E6_002003"
     CDS             complement(12236..13009)
                     /locus_tag="ACG8E6_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5062401.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(13006..13911)
                     /locus_tag="ACG8E6_002004"
     CDS             complement(13006..13911)
                     /locus_tag="ACG8E6_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5062402.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14065..14385)
                     /locus_tag="ACG8E6_002005"
     CDS             complement(14065..14385)
                     /locus_tag="ACG8E6_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFS5062403.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         4676 a         2614 c         3102 g         4263 t
ORIGIN      
        1 ttttaggcga attttaatag aagaatgatt tatttcatca tattttcata caaatgttaa
       61 attttcatac aaatatagga agcgttttac tggcttttta ctatttaaac aggctatcat
      121 aggaaaaagt gacagagaaa gtgaggcgga aaagatgaac aaaaatgacg tactgctagt
      181 tgcaaaaaat aaatcacttt attttttgcc ttccatccgc tctttagatg tttggtccgg
      241 aaaagattta ctacagtcgg aattcattaa atcagctaga caacagttgg caaattatca
      301 gctgatcgtt tttttagatt atggatttaa tatggagatg gctcgacgcg tgcgcccatt
      361 taccaatgca aaaattgtgt tatttttttg gaatcacttc aaagcagaac acgaggcgat
      421 gctgaagcag gcacaagatg aaccggcaat cgatgagatt tatcattttg attttttgga
      481 agcaaaagaa cttgggctga aacataatag ttcattttat tcgaaacata tggatttttc
      541 ttctagcgaa ccaaagacgg atctattttt tggtgcaacg aataatggga gaaaagaacg
      601 agcagaaagc tataagcagg aatttataaa aagagatgtt acgaccaact atttcattct
      661 tccacatcga gggaatgaac aacctggata tcttgcttat gaagagtacc tgaaaaaaac
      721 aggggaatcg cgcgggattc tggaactgtt gcgggagggt cagcaaggag tgactttgcg
      781 gacatttgaa tctatgtatt ttcaaaagaa actagtgacg gataatcaag cgattgctca
      841 ttatcatttt tatcgtcctg aaaatatttt tttgcttcga gaacgtgatt tagatgaatt
      901 accggaattt ctgcatacac cttttaagcc aatcccatca gagctattgg aattttatga
      961 tgcagaaaat tgggcaaaac gatttatcac taacgattcc actatttatg agcaatacga
     1021 atatgacctc tcctctgttg aaaataaata aaaaacctac attttgaaga gattatgtac
     1081 cgaaacaaac aagcacaatt cttatggcat catgagagtt gtgttttctt tttgatcttt
     1141 cattgggctt aaaaaaagag aaccagctgc tgattcagct gattctcatc agagagcctt
     1201 ttattctgcg ccaaggattc cttcttttgc atggactaat tgggtcaaaa aggcacaata
     1261 tggtgtagca atatcatatt tttgtccttt tcgccaaacg gcaccattga tgtagtcgat
     1321 ttcagtcaaa cgatggtttt tgatcaggtc ttggtacata gatgggtaat gcaacccgat
     1381 gttgtcagga tcataagtag aagcaatatg ttggtacact tcttcttgat ctaatatgat
     1441 tccttctttt gctgcgatag aagcaaattc agatacgatt gttcgaacca tgctctctgc
     1501 cgttttttgc gcgcctagtt cagcaatatt acaatccaaa atcgtgcata aaccattcaa
     1561 tgttccattt acacaagctt ttcgccaaat ggaataacga acattatggc tatagatcgg
     1621 atttaatttt gcttcttgaa agacttccac aacttttttt gtaaatgctt cgccttctgg
     1681 ttccaagttc tctagttcga tttctccatc acctaacaat gtgacttttc cgggaccagc
     1741 tagaccagct gtccacatcg tgatcccaaa taagatattt ttctttggca catatttttc
     1801 cagtacatct tcatgaccta atccatttag taaacacaaa acataagtat tgtcagtaat
     1861 aatggattgg atcgaacgaa acatatcatc taattgattc gcttttgtta atgctacgat
     1921 caagtccacg tgttcgttgc tttcgatgat ttcttctggt gaatagattg gcagttttgc
     1981 gaccacttct ttaccgttaa agtcagcaat caacccattt gtacgaatgg cttcaatgtg
     2041 tgcaggccaa cgatcgatca agaggacctc atttccgctt tgatgtagca tcaatccgat
     2101 tcggctgccc attgctccgg ctccagctat tgcgattttc atgttttttc tctcctttat
     2161 caatgagttc ccttttagta tatagaaaaa tggaagaaaa tagcattgtt ttgtttgaaa
     2221 aaaatgaaaa aaagtgtctt acttaagaag aaagtgaggg atacagatgg atattttggc
     2281 ggcaattgat tcatttaaag gaagtgcttc ttcatttgaa ctaaatcagg cagttctaaa
     2341 tggctgggaa gatcgtaaag gaagaaaaat caatgtgccg attgcagatg gcggagaaaa
     2401 tactgcagaa gcaatctatt acgcgctcgg gggaacatgg cgcaccattg atgggatgga
     2461 tcttttattc cgtcagcgaa aaatcagcta tttattgaca acttggcaag gaaaaaatac
     2521 cgcagtcatc gaatctgcac aagcattggg gttagatcta cttgataaac caacggatca
     2581 aactatccga cttgcttcca gttatgcctt aggagaactt atcaaagatg cactgcttca
     2641 agaagtagaa caaattattg tcactttagg aggaagcggg tgtagcgatg gtggtctagg
     2701 attactccaa tccttgggtg cttctttaga agggtacaca gaaggaaatc ctttattatc
     2761 tactaaaaaa cttcattttg atcgaagaaa cgttcttgtt tctaaagtaa aattgattat
     2821 cgctgcagat gttaccaatc catatacagg tcaacaagga gctgcttggc aatttggaag
     2881 acaaaaagga ggaatagata cgacgctagc tttcttagat aaacaagcag gaaagattgc
     2941 tcagcaaata aaagatttct gggaggtgga tttagatgat attccaggaa gtggagcagc
     3001 aggcggtatt ggtggagccc tttttttgtt gggagcgaag atggtttctg gtttcgattt
     3061 agtcagccaa atcactggat tggaaaaaaa gatcgaacaa gcagatgtca tcattacagg
     3121 agaagggcgg atcgatcagc aaacgatcca cggaaaagtt ccacacggtg tagcagtgct
     3181 tgcaaaaaaa tatcgaaaac caataatcgc tatttgcgga agtagagacc aagagctaga
     3241 aggactctct gattttctcc caattgtctt aagtattcat ttgggtccaa taggtttaga
     3301 agaagcaatg gaacacaggc aaacattaga aaaaacaaaa atcttaggac agacacttag
     3361 cagatttttc accatcctcc cgaagtagtt gacaaaatca ttttctttat gctatcgtaa
     3421 tttcaaatat aggacaaagt gagggatccg tgcattgaaa aattaagtcg attgatgaaa
     3481 gctgcattca gactgtacct tgtggagaca aggaagcagt gtgcagattt tcgggattgg
     3541 cttggttttt tcgatgtatg ggtctttttg tgtgtatcca acccttgcat cctctttagc
     3601 cagttctgaa aataggactg gtcttttttt acgaggtgat ggaaaatgtc taaaatcgaa
     3661 ataaaaaatc tgacattcgg ctacgacagc caaggcacat tattatttga acaagcaaat
     3721 ctaaattttg acacacaatg gaaactagga cttatcggac gaaacggtcg aggaaagaca
     3781 actttactga atattctaca aaacaaacta ccttatcaag ggcaagtaat ccatcagcaa
     3841 gaatttgcct atttcccgca acagacaaaa gataaagaac gtttaaccta ttacgtgtta
     3901 aatgatatta cggattttga gatatgggaa atcgaaagag agctccaatt gatgcaaaca
     3961 gatcctgaaa tcttatggag agaattcagc acactatcgg ggggagagaa gacaaaagtc
     4021 ctactggcac ttttatttgt ggatgacact catttcccgt taatcgatga accaacgaat
     4081 catttggata tctctggtag aaaacaagta gcggcttatt tgaaaaagaa aaaacaaggc
     4141 ttcatcgtgg tcagccatga ccggggattt atcgatgaag tagtggacca tgttttagca
     4201 atcgaaaaaa gtcaactgga actttatcaa gggaatttct ctatctatga agaacagaaa
     4261 aaacttcgtg atgaatttga aatggctcaa aatgaaaaat tgaaaaaaga agtcagtagg
     4321 ctaaagaaaa cagcagctga aaaagccgaa tggtctcgtt cccgagaagg agataaaaca
     4381 aagaaacaag tcggattcat cgatactgaa tctagacgag tgaataaagg agcagtgggt
     4441 gctgatgctg cacggacgat gaaacgatcc aaagcaatcg tgaatcggat ggagacccag
     4501 atcagcgaga aagaaaaact attaaaagat atcgaatata tcgattcttt gacgatgaat
     4561 agccaagcgt ctcaccataa gcgactttta agcgtagaag atcttcaatt agggtatgaa
     4621 aatctgttat tcgagccaat tcattttaca atcgagcctc atcagcgggt ggcgatttca
     4681 ggtcctaacg gtgcaggaaa gtcatccatt atccattatc ttctgggggc attcaacggc
     4741 aaggttatag gagaaaaatc ccagccaaaa catctgagca ttagttatgt acgccaaaat
     4801 tatgaagaca atcgaggaac gttggcggaa tttgcagaga aaaaccaagt agactaccaa
     4861 gcatttttga acaacctccg aaagcttggg atggaaagag atgtttttca taacaagatc
     4921 gagcagatga gtatgggcca acggaaaaaa gtggaattgg ctaaatcttt atcacagcca
     4981 gctgaactat atacatggga tgaaccattg aattatttgg atgtcttcaa tcaagaacaa
     5041 ttagaacaac tgatcttgaa cgtgaaacct gccatgttac tagtggaaca tgatcaaacc
     5101 ttcctggata aagtatctac tgagattatt tctcttgaga gaatctaacc cttgtaatct
     5161 caagaacaac aaggtatggt taaggggaac tttgccaacg aaggagagaa tcacatgctt
     5221 tatgtaacga gacacggaga aacaacttgg aacgcacaag gattagtttg cggacgggca
     5281 gatgtgccgc tgactgaaaa aggacagatg caggcacaga aactagcaga aaaggtagta
     5341 gatttgcctg ttccaatcac gaaaatcatc cattcccctc ttcaacgagc aagagataca
     5401 gcacaagcag ttgctgatcg tctcagcttg ccgctgaccg ttgatgaacg attggtagaa
     5461 atggattttg gggattatga tggactgcca agcaaagatg agaactttca aaaagcacgt
     5521 ttagcgttcg ctgtacgatt cccaaatggg gagtcagtct tagatgtgta tgcacggatc
     5581 gttcctttat tgaaagaatg catcgaagac gaagaaaatg tctatctgtt agtttgccat
     5641 aatgccttga taagagtgat caatgcttat tttcatccga tgcctaatga ggggttcttt
     5701 acattcatgg tagataatac agaattggtt tcttatgagt aattatgttt ttgtgttatt
     5761 taaataaaat atttcaaggc actgcttttg cttagcagtg ccttttttag gttatgacaa
     5821 ttcttttgat aatataaact caacctttag aaatgaaatt taattatttt aataaaagta
     5881 ttgaaatttt gtttctttag atatatactc tagtcataga aatgaagacg gatacagaaa
     5941 aagagggata atatgtttgg cttttcaata tttatgaacg attctttgtc agaagagaag
     6001 aaacagtata tccgccaaat ggcatccaat ggctttgtcg gtattttcac ttccatgcat
     6061 ataccagaag atgatgtttc ggcatatcaa aagcgattga tcgaactagg aagcgaagcg
     6121 aaaaagaata aactagaatt gatggtcgat atttcaggag aggcattgga acgagcgggt
     6181 ttttcattag aagatctaac gcctttgaaa gcaattggtg taacaggttt aagaatggat
     6241 tatcatattt ccaatcagca gattgccgac tggtcacatc aattgaaaat cagtttgaat
     6301 gctagcacga tcacgcctaa agatatcgat gaattgaaag aagcagaagc tgatttctca
     6361 cagattgaag cttggcataa ttattatcca cgtcccgaaa cagggctgga caaagaatgg
     6421 tatcagaaaa aaaatcaatg gctcaaaatt caaggtctcc ttgtccaagg ttttgtacca
     6481 ggagatacag agttgagagg cccactatat caaggattgc caactttgga agaacatcga
     6541 gggatccatc ctcttgcagg cgctctagat ctattagcaa gtaacacgga tattgtttac
     6601 atcggagatg caggtctgtc agaaaacgtt caggagcaat ttgcttcttt ccaaaaagaa
     6661 cagacagtct tgctccatac ggaacctgta gatgaagaat tttacgaata tatattaggg
     6721 aagcatacga atcggcaaga cgatgcaaga gatgtcatac gaagcgcaga tgcacgtttt
     6781 cgagagattc cgcctatccc agcaagaaat acagctacgc gtatgaaagg gtctattact
     6841 ttagataacg aaaagtattt gcgctatatg ggtgagatcc aattaaccaa atatgatttg
     6901 ccagcggatg aaaaagtgaa tgtagtagct aaagttatca aagaagagct accattgatc
     6961 aaccaaatca aagctggtat gaactatcaa tttataagaa aagaaggaag ataaaattga
     7021 gcaaaatcaa tttagagaac ctaacaacgg aacgcagaaa ccaagaaact tttggattag
     7081 atgagatgag tatttctcaa gccgttcaac taatgaacaa agaagatcag aaagtggccg
     7141 ctgcggttga aaaagaactg acaagtatcg aaccagtgat tgctaacacg attgagtcct
     7201 ttaaaaaagg tggacgtctg atttatatgg gagccggaac aagtggaaga ctaggtgtgt
     7261 tagatgctgc tgaatgcgta ccgacattcg gtgtagaacc ggaaatggtc ataggtctaa
     7321 ttgctggtgg agaagaagca atgactgtag ctgtcgaagg ggccgaagat tcttatgagc
     7381 tagggcaaga agatctaatc aagctgaatt tgacaaaaaa tgatatagtt attggtatcg
     7441 cagctagcgg tcgtacacca tatgtgatcg gtggattaga ttacgcaaaa tcgatcggtg
     7501 ctgtcacagg aacgatttct tgcaataaac aagccgaaat cagtaaacac gcggatttcc
     7561 caatcgaagt cgatgcaggt ccagaatttt tgacaggctc tactcgattg aagtcaggaa
     7621 cagcacaaaa acttatcttg aatatgattt ctactatcag tatgattggt ataggtaaag
     7681 tattcaataa cttgatggtc gatgtcaaac caacaaatga aaaactaatc gaacgctcaa
     7741 aacgaatcat catgcaagcg acagaagctg attacgaaac agctgaaaag tactttgaag
     7801 aagcagatca aaatgttaag ctggctatcg tcatgatttt gacaaatagt gataaagagg
     7861 aagctcaagc aaaattagct gaagcaaacg gatttgtaaa agggacctta tgagggggaa
     7921 aagaccatgg caaaaaacga actgactttg gaagaactgg caaaacaaat ttataaaggg
     7981 gcaggcggca tgggcaatgt agaaagcatt gtccactgca tgactcgtgt gcgtatgaag
     8041 atcatcaatg atgatcttgt aaatgtctct gaattaaaag cgattccagg agtgcttggt
     8101 gtagtagaag atgaacagct tcaagtcatc atcggacctg ggaaagtgaa caaagtagca
     8161 cagcaaatgg ttgatttggc aggcgtgaaa ttaggagaaa aactttcttc tgtaagtgca
     8221 gctacagcaa attcatcagc atctggaaaa gaccgtgtaa acgaacgggc acaagaaatg
     8281 aaagcggcac aaaaatcgaa acaaaaacca tctaaaatca aatcggtatt aaaagacatt
     8341 tctaatatct ttgtgccaat gattcctgca tttgtcggta ctgggatcgt ggcagggatt
     8401 gccgcagttt tagcaaactt agtcacagcc ggaacacttg atgcagcaac atggcaacaa
     8461 tatatcgatg tgatgaacat cttgaaaaac gggatgttta gctacctagt tatctataca
     8521 ggtatcaatg ctgcacaagt ttttggtgca acaccaactc ttggtggagt tatcggggcc
     8581 gttgtgatgc tgacagggat gaatccggaa gcaccactga aaaacttatt tactggtgaa
     8641 gctcttgcag ctggacaagg tgggatccta ggtgtcatct tcgctgtttg gctattgtca
     8701 atcgttgaga aaaaactgca taaagtagta cctgatgcgg tagatatcat cgttacacca
     8761 actctttcat tgattgccat cggtctgatt gaaattttct tcatcatgcc tttagcaggt
     8821 ttcatttctg atggcatggt cggtgggatc aactgggtac ttggcgttgg tggagcattt
     8881 gccggcttcg ttctaggcac attattcttg ccgatggtta tgttcggact gcatcaaatt
     8941 ttgactccaa tccatgtgca aatgattgat gaaactggtc gtacgctatt gcttccaatt
     9001 cttgcgatgg ctggtgcagg acaagtgggt gcagcattgg cattatggat tcgctgcaaa
     9061 aaagataaag aattaacaga aatgatcaaa ggagctttac cagtcgggat cctaggaatc
     9121 ggcgaaccat tgatttacgg agtaacatta cctctaggac gcccatttat tactgcttgt
     9181 atcggtggag gtatcggcgg ggcagtgatc ggaatgatcg gaaatgtcgg ggctatcgct
     9241 attggacctt caggagccgc tttgatccca ttgatctctg acggtaaatg gttaggctac
     9301 atccttggat tattggcagc ttatgctggt ggcttcgttg ctacattctt cttcggtatt
     9361 cctaaagacc gtttagaaaa agaagaatta gcgactgaaa cagccacagt aaatgaagtg
     9421 cctgaaaaag tagtacctca aacttcagct gaaagtgtga ctatcctctc atcagttgct
     9481 gacggaacag ttgtttcttt agataaagca agtgatccag tttttgcaca aaagatgatg
     9541 ggtgaaggct actttgtaga accagaaaac ggacatatct attctccagt tactggggta
     9601 gtcagctctg tcttcccaac gaaacacgct attgggatca caacgccaaa tggtttagaa
     9661 atcttgcttc atatgggaat caacacagtt gacttaggcg gtacgccatt tgatgtgaaa
     9721 gtaacagaag ggcagcaagt gacacctgat acgcttgtag cagaagctga cttagaagcg
     9781 atcaaagcag caggcaaaga aacatccatg atcgtcttgg taaccaatat ggatcgtgta
     9841 aaaaactttg tcttggaaaa aacaggtaaa gcaaaagcaa aagcacctgt gatggatgtc
     9901 aaagcttaat atgatagaaa caatagaaac gaaagaagaa actgaaatct atttttcagc
     9961 ttcttctttt ttattattta ttgttttctg ctaaaatctg ataaattcgt gtcacatcag
    10021 atggtgttcc ccgtaattca taatctgcca agaaaggaaa gccaaatgtt tgtgcatatt
    10081 gtttggctgt tagacaatac tgattattga agtttttatt tccgctaccg actacaccaa
    10141 gacaaagttt atgattgtct tcatgttcca gatattcacg catcgtttcg gtcaagattt
    10201 ctgtatcacc gttatctaaa ccatttccgc cgtctaaata agtaggaacg aaagtaaaaa
    10261 atggttctgt ttctttcgta aaatcactat tttcatggat ttcttttaaa gtaacttcag
    10321 gattgacagg atcttctgca tgcatttttt cagcatattc tgctagatgt ttggcaaagg
    10381 cacgcgtatt tcctgaaatc gatatatata aaatgttcat ggatcatcct ccaattcttt
    10441 ccttttagta tagcatcttt tttaagtttt ttggtggtgt aatttggaaa gggactaggc
    10501 gctttgcttg tataatgaga aaaaagccgg tagaattata tttctattaa agtttttatt
    10561 aattaagtaa aaataaaaaa ggtttgttat actttttgat aaaagcggtt ttattttgga
    10621 ggatgcttac atggatacag agcaacagaa aaaaggaatt ggtttaattc cacttgctgc
    10681 cttagtaatt ggttcagcga ttggaggtgg cgtatttggt attatgacgg acatctccgg
    10741 atcggctggc ggcccagcta ttttcagttg ggtactcgta ggaacagcaa tgttgatgct
    10801 ctccttgtcg atcaataata tcaataaaaa acgtccagat ttaaaaggcg ggatctttag
    10861 ttacgcagaa gaaggttttg gccgtttcgc tggttttatc agcggttggg gttactggct
    10921 cacatgctgg ttaggcaacg tagcttttgc aacattgttg atgagcgcat taggatattt
    10981 cttcccagta tttgaaggtg ggcaaaatct ggcatcgatc gtgatgagta cagtcttttt
    11041 atggggatat gcatggctag tgaatcgggg agtcgaaaat gctagtttcg tcaatgctat
    11101 cgtcacattc tgtaaattag tgccattatt tttatttatt gtagtagcta tcagcacgtt
    11161 ttcgatccaa caattcattg ataacttttc tagcgacatt atattgaatg cgaatggaca
    11221 gccgatcccg ttttccagtc aagtattgag ttgcatcatg gtgatgcttt gggtatttgt
    11281 tggtattgaa ggagcctctg tcgtaggttc acgagcaaaa aaacaatcag atgttgggaa
    11341 agcaacgatt ctagggatat tcggcttgat tattatttac gtcttggttt ccatgcttcc
    11401 atatgggaca atgacgatga atcagctgca aacgatcaag gcgcagcctg ctatgggcta
    11461 tgtgtttgaa atgatggttg gtaaatgggg agcagtcgtg atcaacggcg gtttgatcat
    11521 ttctttagtt ggtgtttggc tctcatggac gatcttaccg attgaaacaa tgggaaacat
    11581 ggctgaagac ggacttttgc ctaaagcatg gggaaaactc aacaaaaatg gggcaccaac
    11641 ttatgctatc gtattgacga cgttatgtac gaatgtcttt ttactttcat tattattcac
    11701 tgacagtgct tataactttg cttacacact tggtacagca gctattttct tcacgtggct
    11761 tttcttagga ttgtaccaaa tgaaactttc ttatcagcgg aaagaatggg tccaattttt
    11821 agtagggctt ttagcaagtg cctttcaagt atgggctatg ctagtcgtag cctttaatga
    11881 agtaatgctt gttcttgttc ttttcttgcc aggcatttat ttttacctca aagcacgtaa
    11941 ggagcaaaac gagcctgtac aacatatcca cttagataag attttactta taggtgtgac
    12001 gatggtcgga gtgattgcaa tggttttatt tgccactggg atgcttcacg tatagaagta
    12061 taaaagaagt ataaagagag gttgtgagcc tgtcgtttat tcaaaaacat actaggaaag
    12121 gagctgtgac acaaatgttg tcacagctcc tttttcagaa taaacggtgt ttaagagaca
    12181 gccaatcggc aataaggctg aacgtctctt tcacagcctc ttttttgatg agtcatcata
    12241 cattttttcg cttaaatacg agatatccta atgctaagaa caaaacgatg taagctaaat
    12301 taccgaatag catttgatta gtagaaagat gagtcaattg cttcatgatc tcttcgtttc
    12361 caatttggtt ttgcagattc aacatactga tcggattcca tttgatccac tcccattttt
    12421 ggatgagtgc aataaggata ccagatatga ttgacgaagc aaaataaaag acgattcctg
    12481 ctgagatgga tgcaccggaa ctattgataa agcaagcaat catcaacact aaacttaaaa
    12541 tcagccataa accgatataa ctacccaaag tataagtgaa caagctttgg atcagtgttt
    12601 gaccagtaga aaccttctcc gtgaaggaaa tcgaagggaa aagaatcagt ttcatcaaca
    12661 cagtgacaat aatagctaaa aggtataaat aaacagaata aataactaat gtgatccatt
    12721 tgcttactaa tatttctcct cgagaatact ttcgataaag gagatttttc aatgtaccat
    12781 attgtgcttc catcgaaatg atcgtgcttg cagctgcaat catgataaag acaatccaag
    12841 atgtggcact aaaaagctga gcagtcattt caactggatc aatgatcgtc gtgtattttt
    12901 tggctagaag tgctgttcct atgagcaaaa caactaatag aacagataaa acagcagttg
    12961 actttttctt gatcaattta aataattctt gttttacaag caatgtcatt tttcttcttc
    13021 cccctttact gaatccagaa tttccaatag agaagattcc aaatcattcg tcagatgctg
    13081 gacgtctgtc acatggatat tttgtgcaga caatagctgg atcacggttc ctaataattc
    13141 ttcgttttct tttaaaagct tgattttatc aggatcttcc aaagtatatc cagcttctgt
    13201 caagatcgtt cgagcagcta gatcatttgt tgtagagata acgatgaaat cttgcgctgt
    13261 ttggttcaat tcagccattg tacattgttt gatgatcttt ccttgattga tcaggatcag
    13321 ctcatcgacc attttttcaa gctcgctgag taagtgactg gagatcaaga aagaggtgcc
    13381 ctgagtagcc aagtcggtaa tgatttttcg cacatctctt gttgcttgtg gatccaaacc
    13441 gttcatcggc tcatctaaaa taacgataga cggatggttc agtaaggcaa gagcaattcc
    13501 caatttttgt ttcattccca acgagtagga tttcgctttt ttgtgaatgt acttttccat
    13561 cttgagctga ttcacgatag tgtcgatagc agatacatcg ttggtttcag agaataactt
    13621 cagatgatca tagcctgtta agaatggata gatacccggg tattcgatca atgctcctac
    13681 ttgttccaac cctttatgac tagagggaga aatctcattt tcaccaatac agattgttcc
    13741 atctgagtaa tggatcagtc ctaaaatagc tttcatgatc gttgtttttc cagcgccatt
    13801 aggacctact aatccaacga ttttgccagg ggataagcta aaactgacat cgctgagggc
    13861 ttgtttgtga ccaaagttct tggataattc ttggaccgaa agacttttca aaaatattca
    13921 ctccttttct atagatagta caagcataca cctgatgagt ggagaagtcg ctaggtcatt
    13981 ggacttattt cttatttttt cataaaaaaa ttgaatatta aataaaaagg cttatttttt
    14041 ctgatcagta cttattccaa gtgtctagtg gccgctagta atcttgatgc caatgatact
    14101 gatgagtagc atccccacaa agaaccaagt ggaaagattc agcttatcat gaaacaagac
    14161 cacaccaatt atgaccgaac caactgcgcc aatccctgtc cagataggat aagagagact
    14221 catcggcaaa cttttgattg cctttgacaa aaagtaaaaa ctagcaatca tgccaatcac
    14281 tgtgtaaaaa gaataattga ttttagaaaa accatcactc catttcatca tcgttgacca
    14341 gaatacttcc aaaattcctg ctatgattaa ttccatccat gccatactta cattcctcct
    14401 taactataaa aaaatacaca aaaagaggaa aatcatccct taaaagacct aatggaatga
    14461 tcatcctctt tatcctaccc ggtggcagat cgtatatctt tttctgtagt atagcacacc
    14521 tatccagata tcggcaatct catacttttt agtaaagtgt accaagcact gacggatata
    14581 tcgttagcgg aaaagaagat gaatgctttg caatttcata gaaagaagtt gttatttatt
    14641 aatcatttaa aaaaa
//
LOCUS       DAZUMM010000066        13900 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000066 DAZUMM010000000
VERSION     DAZUMM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.068"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..175)
                     /locus_tag="ACG8E6_002006"
     CDS             complement(<1..175)
                     /locus_tag="ACG8E6_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS5062404.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLA"
     gene            complement(654..1373)
                     /locus_tag="ACG8E6_002007"
     CDS             complement(654..1373)
                     /locus_tag="ACG8E6_002007"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS5062405.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1422..3068)
                     /locus_tag="ACG8E6_002008"
     CDS             complement(1422..3068)
                     /locus_tag="ACG8E6_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFS5062406.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3247..3837
                     /locus_tag="ACG8E6_002009"
     CDS             3247..3837
                     /locus_tag="ACG8E6_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5062407.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3929..5434
                     /locus_tag="ACG8E6_002010"
     CDS             3929..5434
                     /locus_tag="ACG8E6_002010"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFS5062408.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5518..6870)
                     /gene="celB"
                     /locus_tag="ACG8E6_002011"
     CDS             complement(5518..6870)
                     /gene="celB"
                     /locus_tag="ACG8E6_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS5062409.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6870..7388)
                     /locus_tag="ACG8E6_002012"
     CDS             complement(6870..7388)
                     /locus_tag="ACG8E6_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062410.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7404..7730)
                     /locus_tag="ACG8E6_002013"
     CDS             complement(7404..7730)
                     /locus_tag="ACG8E6_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFS5062411.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7743..9722)
                     /locus_tag="ACG8E6_002014"
     CDS             complement(7743..9722)
                     /locus_tag="ACG8E6_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5062412.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9743..10057)
                     /locus_tag="ACG8E6_002015"
     CDS             complement(9743..10057)
                     /locus_tag="ACG8E6_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFS5062413.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10225..10518
                     /locus_tag="ACG8E6_002016"
     CDS             10225..10518
                     /locus_tag="ACG8E6_002016"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFS5062414.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10596..11708)
                     /locus_tag="ACG8E6_002017"
     CDS             complement(10596..11708)
                     /locus_tag="ACG8E6_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5062415.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11861..12709
                     /locus_tag="ACG8E6_002018"
                     /pseudo
     CDS             11861..12709
                     /locus_tag="ACG8E6_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13062..>13900)
                     /locus_tag="ACG8E6_002019"
     CDS             complement(13062..>13900)
                     /locus_tag="ACG8E6_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5062416.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
BASE COUNT         4336 a         2837 c         2382 g         4345 t
ORIGIN      
        1 tagccaagaa cccatctgct gaaatatgaa aatttttgaa tgctacgaca ttcgccatta
       61 caggtatgta aaagaaaaaa atcatccaaa gcatacccgg tacagccatc aggacaagtg
      121 ctttataacg ccatagattc ataaaaaaat gattgaaccc ctttcgtttt ttcatctaaa
      181 aacccctttc gattcattcg caacgtaatt atagcgcttt caataatgag aataaataaa
      241 caaagtatag ggaaaatgaa acaaagtata ggagaggttg tgaaatcagc gttttggcct
      301 gagtattaga gaaaaaaacg ggaaaatagt tcctcatatt ttgccgtttt ttgagctaat
      361 accgagggtc agctgattga acaccgttta tcagaggttg tgaattcagc gttttggcct
      421 gagtattagg gagatcgtgt ccttactatc taactctgag tcacaaggaa caatggggac
      481 tgtaacaaaa gtcttgtcac agtcccttat tccgaacaaa cggtgttcca gaagtagctt
      541 ctacggaaat aagccgaaat ttcacaaaaa agccatccga aagtcagacg gacaagtctg
      601 cttttcagat ggcgctacaa ctactctgct tattctttta gctatccatt caattatttt
      661 tctctcactt gttctagctc ttctttggtc atgggtcgat attgcccttt attcaaatct
      721 ttaggcagcc aaaattttcc catacggatt ctttttaaat ataagacttc ttttcctact
      781 gctttgacca ttcgcttaac ttggtgaaac tttccttcat gcaaaatcag acggataatt
      841 gaagtttctg tctcttgatc tacttgatca atttggagtt ttgccggcaa tgtctgctca
      901 tcgccatcaa taatcagtcc ttctgcaaat aaacggatat cttcttctgt cattataccg
      961 ctaacctcag ctagatattc tttctccaca tgttttttag gtgatagtaa ctgatgggca
     1021 aacaccccgt cattcgttaa aatcaagagg ccctctgtgt ctttatctaa tcggccgact
     1081 ggaaaaagat cttcccgata atcctggtct tccaataaat cgattacggt ttgatcatga
     1141 ttatcctgag tagcagaaat gactcctgca ggtttatgca aaatgtagta aaaatctttt
     1201 tgatacgtaa tgatttctcc taaataagta accgcatctt cgaattcatt tacttgcgct
     1261 ttatcgcttt tcaccacttg tccattgact tcgatcgttc cttttttgat taatcctttg
     1321 acttctttgc ggctgccaag tcccatatct gctaaaaact tatctaaacg catgcgtttc
     1381 ccccatttca aaagagcatc aatctagctc ttctgattca cttattttat ttttaattta
     1441 tgtcgtaaac gcgctattcc tggtcctagt agtctttctg ccaagcggat tttcaaagct
     1501 aaataaccat acactaaagc ccctatacct gctactacaa gaatcaacaa tagagactgg
     1561 aatttgctat cttcgtttaa aaaagcacca aaaatcatct ttgtgatcca cgaagccaaa
     1621 agcataatca aggtaagaat aaagatcagc aatgtcctgc gccagacaaa acttggttta
     1681 aagcgggcta cttcatggat tctctttaag atcaaggtac aagacacagc aaatccgatc
     1741 attgtcgcca ataaagggcc gtatacttca aataaacgaa tggctgggta ttgaagaagg
     1801 agtttcacta gaaacccaat aaccaaataa ttaattgccg ccttgttttc aaacattcct
     1861 tgcagcatgt tggaaactag catatatagt cctaggaaga gtcctacaaa acttgcttgg
     1921 atcagtacat tacttcctag agaatccggt gtataaaata atgtatttag cggataggca
     1981 agtaacataa caccaaatgt cgccggaaac ataaaaaatg aaaacaactg taaatttccg
     2041 cttgtcaatt ttgccagacc tactcgatct ttcaacgtaa cagcttctgt aatcaacggt
     2101 aacccagtag acgcgatcga tgttgccaat gcaataacta ccatcgtcaa cttatctgga
     2161 ttggcactaa aaatcgaaaa aagatccagt aactgtgcat tggaatactc ggtggatgca
     2221 gacatgatct tcataaaagt gaactgatcg actaatttga agatcgttac ccctgaaccc
     2281 acaataataa acggaatcgc ttctttaacg gtatctaaca acaattcctt cgttgcaata
     2341 tttattttgt tttcgctgta gtgaataaac gccgatgtat acgcttgatg tttcttcaaa
     2401 aaatacaaaa ggacaagaat acttgctagc ataccgataa atgccgcgaa agttgattgc
     2461 gtgaccgccg tcacatagtc accatccatg actttcatga tgatgaatgt agccaatagc
     2521 atataaaaaa tacgtgccac ctgttctaca atctgcgaca gcgcataagg catcatttct
     2581 tgattgcctt gcaaataccc tcgtatcaca ctcatacaag gaaaaaccaa tacagcgata
     2641 ctcaaagaac gcatcgttgg aatcagttct tctccgccac cagaagcatg agctagccaa
     2701 ggagaggcga tgtacatgaa caaagcaaac aacaggccta acccgcccat catctgtaat
     2761 gcacggataa ataatcgtcg tgaagtgcca tattcattca atgaattata gcgtgcagtt
     2821 tgttttgcaa tcgcagcagg tattcctgct gtagagatca gtagaaacaa tgcatatata
     2881 ttgtatccca tattgaataa tccattggca gccttagcat tttcgcccat ccaagcatac
     2941 cagggaatga tatagacagc gcctagcaat cttgaaatga tattactggc ggtcatccaa
     3001 gcagagcctc gtgccatttt ttcttgcggt gttaattgat ctaatgctgg ttttctttga
     3061 ttatccatcg gtcagattcc aactttctac aaaacattac tatcatttta attggtaaaa
     3121 cagaaactgg caatcgattt tatataactt ttataagaac aattgttttt ttgaattcgg
     3181 tttcgttttt ttggtaaagt aaagaaatat acagcctttt atggctaaca tttataggag
     3241 atatcaatga cacaagaaat aaaaaaacta cccgttatat ttttaggtct tttctttatc
     3301 acagttggat tgaattggtt cttgctccct cacgatatag cttccgccgg agtgggcgcc
     3361 atcggacatt tagttgaaac ggccttttct atccaacgct cagttactgt ttggagcatc
     3421 aatcttatga tgctgcttct agccgctgtc ctattaggaa aatcagtatt ttttaagact
     3481 gtagcgggaa gtctgctttt tcctgttgtt ttagagattg ttccaaaagt ggaaatgatc
     3541 tcctcacatt tcttttcact gatttcagga agtcttttgt tcagtcttgg ggtttacaca
     3601 ttgtacacag tcggggcctc aaatggcggt ataacgatac cgccgattat tttccacaaa
     3661 ttttttcggc ttccaatgcc gcaaggactt ttacttacca atagtcttat cgtctttttg
     3721 aattattatg tttttggatt gttggagaca ctattcgttc tcctatctat tacagtcagt
     3781 tcattgttta tgaaaatact catccgcttg tcccctgtgt caagaaaatt tgaataaagc
     3841 tggattttgt catagcggaa acaaacgata tattaaaaag agtgtgctat aataaaccaa
     3901 gaaattgaat aaagcgaggg atcaatcaat gagcttctct ttagaagaca tccgtcattt
     3961 actattaaaa gaaaatctat taaaagaatt tgtttcaaca agaggctggc atcttgatgt
     4021 accagaaaaa gctgaatttc ttcagttaag ttatgattca agaaaagctg atgcttctac
     4081 tttgtttttc tgtaaaggaa tgaatttcaa agaagaatat ttggattcag caatcgaaca
     4141 gggtattcaa tattatgtat cagaacaacc atacgaaaat tcagcagtag gaatcatcgt
     4201 aacggatatc cgtaaagcaa tggcgcttct tgctatggct ttttacgact atccgcaaaa
     4261 taaattgaaa gtgatcggat tcactggaac aaaaggtaaa acaactgctg cgtactttac
     4321 aaaagcaatc ttggatcaca caacaaataa aaaaacagca ttgctttcta ctatgaatac
     4381 aacgttagat ggcaaaacat atttcaaatc ccatttgaca acgcctgagt cattagattt
     4441 atatcgtatg atgtcagaag cagttgaaaa tggtatgact catctagtga tggaagtttc
     4501 ttcacaagct tacaaaacac aacgcgtata cggcttgact ttagatgtcg gaatcttctt
     4561 gaatatctcg cctgaccata tcagtccgat cgaacatccg acatttgatg actatttcta
     4621 ttgcaagcga caattgatct tgaattcaaa aacagttgtt ttaaatcatg aaagtgacta
     4681 ttttgattta cttagggaaa cagccgatct attccagatt ccaacgatca cttatggtcg
     4741 ttcggaagaa agcaattatc aagtgatccg ttcagataaa ggtactcacg gttttacttt
     4801 atcttctcat gaagatcgct tagctatctg cgatactgct tatgatattt tgctggctgg
     4861 aggattcaac caagaaaatg cagccagtgc gatcattgcc gcagcactaa caggtgcctc
     4921 cagtcaagat gcacaagacg gattaaaaga agctcgtgta ccaggccgaa tggatcaatt
     4981 gatccaatcc aatggagcgc atgtctatgt ggactatgca cataattatc taagcttgaa
     5041 aacattgctg aaatttgcca aaaatgaaca tccagatggc cgtgtaattg ttgtattagg
     5101 cagcccagga aacaaagcaa tctcccgtcg acatgatttt ggaaaagtgc tatcagaaac
     5161 agctgacatc gcctttttga cggcagatga cccagcattt gaagatccga aaaaaatcgc
     5221 ggaagaaatc aatgaagcaa ttactaatcc agatttgatc gttcattatg aaatggatcg
     5281 tcctgaagcg attcgacgcg ctttagcaga aagcgctccg caagattcag tcgttattgc
     5341 gggaaaaggc gtggatccct atcaaaaaat caatggtgta gatgaacctt atgaagggga
     5401 ttatgcaatc gtgaagcggt tgattgaaga ataatggtat ttatatacta gtggtttccc
     5461 atttctaaga aaaaaaggga ctgtgatttt ttgtcacagt tccttttttc ttagaaatca
     5521 ttttaacttt tcactagcta attctgctgc cttttctctt tcttcattta ataatttatt
     5581 atcatagaat ttgataaatg ggaaccagat aatgaatgcg atgattgcac aaatgactgc
     5641 taacacagca gctttccaat caccaccact accaacaaat gctccgatcc cgactggtga
     5701 aggccacggc atctgtgcaa gcatcggttt gacgatttcc agccgaatgg caaaataagc
     5761 gagtgtagca gaagccatcg gagctaagaa aaacggaacc gctaaatagg gattgtagac
     5821 gattggcatc ccgaaaatga ttggttcatt gatattgaat aatcctggta caagtgaggc
     5881 ttttcctaac actttcagct gatcagattt cgcgaggaat gcaataaaga tgatcaatcc
     5941 taaagtcgca cctgatccac caacggttac ataagaattg ttgaattctc ctgccaacgg
     6001 ataatttgcg ccttgtgcgt tagctgccat attagctaat acgatcgggg tcaagaaaga
     6061 agtgatgata ttcgctccat ggatccctac gatccacaat gcatggatca agaaataaat
     6121 caccatgaca ccaagccatg tattcgtcaa ctgcgtaaca aacccaaatg ggatagcaat
     6181 cattttgaag atatccgttc ctagtgcaac taataatcca ttgatgatca tcaccacaaa
     6241 cgcaatgaca aatgttggga tcagagcagt gaaagaacga gaaactcctg gcggaacaac
     6301 atctggcatt tttatgaccc aatttctctt gacacacata caatacaatt cgaccgcaat
     6361 aatcgacata ataatagctg taaagattcc tgaagttcct aaacgttcga caaaagagcc
     6421 catcctaata ccatttacta cagtttctgt ctcattgata gaagcaatca agctgatttt
     6481 accatcagtc atgatcaatt cagggataca cataaagaat gcaaaaacag atagtaatgc
     6541 ccccgtgagc ggattaacat ttaatttttg ttcatctcgt tcaattgacg ttaattcata
     6601 agcaaacaca atggcaaagt aaatagataa aatccccatc gtcattgtat tgacgatcat
     6661 gtacaagtca ctaactttga aaaaagttgc attaaaaaat ccttctaaaa atgggaacgg
     6721 taatggcaaa atatttaaaa ccaaaaacat cgagcctaca atcgtgaaag gaatagaagc
     6781 catccccgct gccatgacag cccgaacgat tttaagttgc gcaattttcc ccataggtcc
     6841 catcaggtat ttctgcaaaa aatcaaacat tagttattct cctttcctaa atataacgct
     6901 ttttcatatt gtttaatacg gatatactgt tggtcttggt tgagtgaaat tttctttaaa
     6961 cgatactgta taaatgcact ggcaaaaata ccgcttacaa caagaatgaa aacaaataac
     7021 tgtcctccat tgttatttgc cataaaagga tacaagaatg taaatcctga ggtaaacaac
     7081 actggaatca ctaataaatt aaagacactc tggaacaaaa aataccactt tgtccatgga
     7141 acgttcgttt ggtgttttcg ataaagcttt acttgttctc ctactgcagg caaaatagct
     7201 aaaatcagaa tcatcggtaa actacttcct aaacttttag ctgatagaag cagaatcatc
     7261 cattgcatgt tcatgaaaaa gaaaaaggca gtcaggtaac gtacgattag ataacgattg
     7321 aaatacatgt ttttcaaact aagttgcttt ttttctaatt cgattttcct ttttactgta
     7381 tcctgcacaa tgatccttcc tttctattta tttttttgat acaaataatt catttcctgt
     7441 gcaacttcca ataatgtcat cgtcgtcatc agatgatctt gtgcatggac catgataatt
     7501 tccatttcta tttttgtccc gctggcatat tctttcagaa gttgagtttg tgactgatgc
     7561 gcatgaagaa tctcttcatt tgcttgtcgc aacttctctt ctacttcttc aaacatctct
     7621 ttacgcatcg agcgaaatgc ttcatgtatc agtgtccgtg catttccgct atggaaaatg
     7681 atatcaaaag aagtcacttg tagtgtttca ctgtttattt tttcagccat ctgttttcct
     7741 ccttattcga tcagtttgat aaatttactg ctgaaattct caaaaatagg ttcagctaag
     7801 atttcctgct gtacttttag ctggtcaact aacctaacga ttgctttagt cacgaccgta
     7861 attccttcat tttcgacata agaaggcgaa actaaaaaaa caaaatgaat ttcttgatat
     7921 tgatcccact gcatcccaga aggaatcaat gcaacggcta cctttgtttc gattccaact
     7981 ggaacagcgg gatgtgggac tgccactgta tcactgaata tgatttgtcc catattttca
     8041 cgttgctgga tctgctttcg catttctctt atatatcctt cttcttcatt tatgctcagc
     8101 ttttgcagca aatcatgaag aatttcttcc tttttcggtt cgtacgtata agtcttgaaa
     8161 aatttttcag acaactgttc ggtgtaaatc tgcttttttt gctgtagaga aaatgctggt
     8221 atcggtctat ctgaggaatg atagggtata gattctccaa ctacctttcg tattttacga
     8281 acatcatctt cattcaaaaa aatactgaca tgtaaaactg gaattttgaa aaacattgtc
     8341 gaaagatcaa tagaagaaat aatcaggtcg attcctttta aagttgtttc attgatctca
     8401 taatagcctt ttacacttac cactgtaata tttttaccga attcactgac tacgcgattc
     8461 tttaatagtt gggcactgcc gtaacccgtt gcacagataa tcaatgcatg tagtttatgc
     8521 gcagctcttt ctttttccaa tgccgccatg agatgcaaag ccaaataggc ccattcatct
     8581 tcattgatgg tatagttttt caaagccggc atgttgctga aatgcctttt tactgcgtca
     8641 aagactttgg gattttcttt ctttatctcc tcagtcaatg ggttctcaat gcgaatacct
     8701 cggctcaatc gaatcatcat cggtgacaaa tgatcgagta gtccattgaa aagctggaga
     8761 tcctctacta atgaaaaatg aaattcctga gacaatcttt tcagaacttt cgctaattcc
     8821 tttgatagtt cctgatcatt ttgattttct ttgtgattcg acttagccat gagatgtaat
     8881 gtcaaataag caatttcttc ttcgggaaac gatacacctg cgattacctc aatacgttga
     8941 acgatccgct gcgccacttg atactctact cgcttagaaa cttcttgttc gatgcctaat
     9001 tccttgattt caaacccttc tcggagccgt ttgatcccca aagccaagtg taaagtcaga
     9061 ttctgtatga taaaatccga aacttttaac ttagcttctc ttgtttcatc gagaatgata
     9121 atgactagct cttcaaaact gatgtctggg aaaaaacggc tttgccctaa atattctttc
     9181 aatgaatttc cataatggtt tccaaaaaaa gtatccatga taaaatgccg tcgatttctt
     9241 tcttcccctt cgatccatgt cccttgacca tgcttagaaa cgatttttag tgaatatgga
     9301 atcaatcgct ccttgatttc ttgcatgtcc tttgtcaaac tggaacgact gatataaagc
     9361 tcttctgcca aatcatccat aaaaagaatg gcatcttcca aaagcaattg gttcaatata
     9421 tacttttgtc gatcttcgga ctcataaaat ccttgcggtt ctctagcata tccaggtact
     9481 atttcttgcc gactcaaaaa caagtcaaat gtcagtcttt gtaaaatatg cagttggtag
     9541 ccataacctg gtttagcaat gatcttacct ccgttttttt ctactaactc tttgatccgc
     9601 tgcaagtaat tacggattgt ccgatcagat aataacaatt cagaagctaa acgttgactg
     9661 gtgacatact ccttcttgtg atcaatcaaa tatctcaaca gcaatttttc cttctgtttc
     9721 attatcatcc actcctattc catcatatgt tctcttcgat cacctctgct aacttctgga
     9781 tgcctgtagg gattgggaca tacgcttgga aagggatact aacaacaggc tttccaacag
     9841 attctcctaa ttttttgaat ttatccaaaa acatcgtcgt ttgcgggctg atcagaaata
     9901 aatcaaaatc actattctta atcatattat cgccttctgt agctgaaact gcatctacga
     9961 gtacctcttc tcttttttgc ttaaagaaat ccgttgtctt tgaggccatc atcgaggatg
    10021 acattcctgc cgcacaaata atcaatgctt ttttcataaa tcaatcactc cttttatttt
    10081 tataaaccaa gcatatcttg taagcgtttt ctattcaaaa tatatatttc cgtagcattg
    10141 cggaaaaaat gtgtatcata tcttgaataa taatttctgg caccgtgata tactagtgat
    10201 agaatttttt caggagggga tttagtgagt atcacagtga atattttgta tacaagtaca
    10261 aatggacaag ccagaaaatt tgcagaagaa atggtcgaaa gaaacattgt caaagcgatt
    10321 cgtgcagaag aaggaaacga gaaatatgac tattactttc cactagaaga tccagaatct
    10381 cttttactga tcgaccgttg gaaagatgaa gaagcaatcg aaaagcatca caaatccgaa
    10441 atgatggctc aaataaacga actccgtaaa aagtataaat taaaaatgaa agtcgaacgt
    10501 ttcactgatt taccgtaaaa agatacacat ggaatgatag caatttatca tccatgtgta
    10561 tcttcacttt ttccttattt tttattcgat ctcatctaat gattcaaacc gtgtttttct
    10621 cttatttttc ggataagtat ttcagtcacc ccaatataca tgattcctaa acatacccca
    10681 aggatattct caaaaatgcg gatcgttact gctcctggaa ccccgaataa actggcagtg
    10741 attgccaaag caccaaaaga attaaaaact gttttatatc tataggtaga acaaacacct
    10801 aaagctagac cacctaatat accgacccag ttaaaaggaa taaattggct gataccaata
    10861 aataaaacac atccaatcaa tgagccaatg attcggtcta ctgctcgttc tttgaaacca
    10921 atcgacatca atccatagct agatacaata gaagaaaatg caaaccctgc ccacatcaat
    10981 cgctgaaaag ggacatactc tcctgcaacg atcaaaagac taatgccaaa tgcatagatg
    11041 agcatccaaa ttttttcctg cgaatacatg cctttcaaaa aaatctcttt aaacaagctt
    11101 ttgtctttgt tcttttctcg atgttttctg taaaggagaa ctgaaaacca acaaaaaaac
    11161 aaaaataata agctacttgt ttgtgtaaag aaattcttgt tcaacgaatc tttaggtaac
    11221 gaataaacaa taaagagata tgaaaagccg tacaaagaag cattccccat tttgggatca
    11281 ctagcggttg ccattaatat gctgcttagt aaaataaaat gaatcaacat gcccataact
    11341 gaccatttga tcagctggac atacggtgct actaatagtg agaacatgac gattgctaaa
    11401 cctatgatcg aatgggaaat cttataacca aagtcaacaa aacgaatact catcaacata
    11461 cagaataaaa ccacagcgta tggtttatgc ttcgattcaa ataatgttgt aattataatg
    11521 ataaaacaaa tggcaaacaa tacgatgaat aatgatcgta aaaacaatga acaaatatac
    11581 atgcactttt cttttttaga agctgcttct cgtatttttt gttttaaaat aaacggatct
    11641 agttgtagag actgataaaa atccacctgt tgtcgtcctt tctttttact tttatttttt
    11701 acagtcatct atgacaggtt gtaaatacta tggaacatca tatggcgttg tcattttctt
    11761 gtcaactatt tttcctattc atgtttttat aaaaaaacat atcgttttct tgtatgatta
    11821 aatcaccaca aaataaacaa ctgagagaac gatatgcttt atgaatggtt ctatcaaaaa
    11881 aattctgaga tcaatagaca aaaatttgat gattacagag gtctcttacg aaaccttaca
    11941 gaagaaaaaa acattgatcg ttgctgcagt tctcttccct gctccatgtg tctgtagaaa
    12001 ttgtggttct gctgtggtag atggaaatag gaaattaatg gtggtgaaaa acaagaagaa
    12061 ggaaaccatt gtccactttg aataatataa tcatatgcca atggttatac gcctaaaaaa
    12121 gcagcgctat acctgtaaaa actgtcgaag ccattggaac gctcaaagtt attttatccg
    12181 tccaaggcac tcgatttcaa atcatgtcag acataaaatc acttctttac tgactgaaaa
    12241 agtgtcgtta ttttttattt cgaaaagttg ttaggtatct ttgacaaccg ttattcgcac
    12301 attgaaagag ttgagaagct atttaccaaa gtcttccaaa agaattctgt cttgagtgtt
    12361 gagggttgtg aatttcgttc tcacgtttcc gttgaggata aactgagttt tatttccgcg
    12421 gatggcgaga cagaaaaatt aatcgagaaa atttacctac acaaaaaatt cctcgattaa
    12481 caaactattt ccttagctgc actaatccta aagaaataga attcttagtt acagacatga
    12541 acgccaccta ctttcagctc accaaacatg ttctaccaaa tgcgaaattg gtgattgatc
    12601 gattacttgg tttttccacg cctttcaagg aggcgtaacc tttattcacg agttggttga
    12661 agctttccga gacaaaagac ccgacttgtt tttctcctta ttgacaaaga gccattaaaa
    12721 ctagagaaaa aattacaact gatcgctgat tatttgtaga aagaaaatca aagtattacc
    12781 caataaactt gagtcattag gcaaaaaaca ctgcgaaact caagaatgat tttcgcagtg
    12841 tttttgtata ttttttatta actagtatta ctacaaaggt ttatttttca cttctcaacg
    12901 cagcacaaaa ttgtctttac ttatttactg gttgggttca accttctcta ctccgatact
    12961 aatatttgtt tgtatacccc ttctaaaatc ggtagtttcc attcaattga tgggtactag
    13021 ttgattttaa aaactatgct tattcaattt tttattttag ctcatatatt tcgtttcttt
    13081 ctaaatttta tactgatgaa aacaagtagc acacccaata ttgacaatat aaactggcta
    13141 atgatggttt ctccagtttt tggaagatgc ttatttccac tgcttgttgg attgtttcct
    13201 cttatatctg agctatcagc aacttttcta ttctcttttg ttaaaaagat cgccacttct
    13261 tgttcttttt cgatcttgaa agttactggt gtcgcatcca attcataccc cgttggcgct
    13321 tgagtttcta ccagctgata tgctcctggt gctaagccat ctatcgccag ttttccatct
    13381 gctcctgtgg ttagacctga ctgcagaacg gttccattgg catcttgtaa ctcaaaaaca
    13441 gctccttgca ggacttctcc gctttggtca tcgatctttg tcaacacgac accgcctggt
    13501 gtaagtcgat tttctttcgt caattctacc accgctgtct gacttctttc gatctcaaac
    13561 tcgattggtg tcgcatccaa ttcatacccc gttggcgctt gggtttctac cagctgatac
    13621 actcctggtg ctaagccgtc aatcgccagt tttccatctg ctcctgtggt tagacctgac
    13681 tgcagaacgg ttccattggc atcttgtaac tcaaaaacag ctccttgcag gacttccccg
    13741 ctttggtcat cgatctttgt caacacgaca ccgcctggtg taagtcggtt tgctttcgtc
    13801 aattctacca ccgctgtctg acttctttcg atctcaaact cgatcggtgt cgcatctaat
    13861 tcataccccg ttggcgcttg agtttctacc agctgatatg
//
LOCUS       DAZUMM010000067        13726 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000067 DAZUMM010000000
VERSION     DAZUMM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.069"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(142..1344)
                     /locus_tag="ACG8E6_002020"
     CDS             complement(142..1344)
                     /locus_tag="ACG8E6_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5062417.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1347..1541)
                     /locus_tag="ACG8E6_002021"
     CDS             complement(1347..1541)
                     /locus_tag="ACG8E6_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS5062418.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIISVPLSQEE"
     gene            complement(1588..2436)
                     /locus_tag="ACG8E6_002022"
     CDS             complement(1588..2436)
                     /locus_tag="ACG8E6_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFS5062419.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2641..2991)
                     /locus_tag="ACG8E6_002023"
     CDS             complement(2641..2991)
                     /locus_tag="ACG8E6_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062420.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3169..4032
                     /locus_tag="ACG8E6_002024"
     CDS             3169..4032
                     /locus_tag="ACG8E6_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5062421.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4656..5648
                     /locus_tag="ACG8E6_002025"
     CDS             4656..5648
                     /locus_tag="ACG8E6_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5062422.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5664..6548
                     /locus_tag="ACG8E6_002026"
     CDS             5664..6548
                     /locus_tag="ACG8E6_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5062423.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6597..8204
                     /locus_tag="ACG8E6_002027"
     CDS             6597..8204
                     /locus_tag="ACG8E6_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5062424.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8205..9689
                     /locus_tag="ACG8E6_002028"
     CDS             8205..9689
                     /locus_tag="ACG8E6_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFS5062425.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9866..10858
                     /locus_tag="ACG8E6_002029"
     CDS             9866..10858
                     /locus_tag="ACG8E6_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5062426.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10910..11779
                     /locus_tag="ACG8E6_002030"
     CDS             10910..11779
                     /locus_tag="ACG8E6_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5062427.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12199..13725)
                     /gene="guaA"
                     /locus_tag="ACG8E6_002031"
     CDS             complement(12199..13725)
                     /gene="guaA"
                     /locus_tag="ACG8E6_002031"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS5062428.1"
                     /translation="MVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPKGI
                     ILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAE
                     LEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYG
                     IQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLS
                     GGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKER
                     FLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTET
                     AQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLG
                     IRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYD
                     YTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE
                     "
BASE COUNT         4213 a         2321 c         2832 g         4360 t
ORIGIN      
        1 tgaaaaacgt tgatttatcg gcattcccca tccatattat atttacgtct tattcccact
       61 caatatgagt agcctcgtaa agaagctatt atatcagtgc tttttcagcc tattcctcat
      121 tttggtaggg aaatggtagg gttagatttt tactgcttga tttccattaa agtttgcaac
      181 tttagatttt gttgcacagg taaatttaat gtttcctgtc atttgttctg caattccttc
      241 atcattacgg ctccatagat gagcgtagtg ctttaaagtg atttcagggc tagaatggcc
      301 taaccgttgc gacacgacca gaatgtctgc gttaaactca ttgattaggt agctaacatg
      361 agaatgacgt aatccttttc cttgtattag gggtacctta gctagtttgg cataacgctg
      421 aataattcgt tgaacagttg aacgatagag tgggaggtca gtgtagctaa gaataaattg
      481 ttctacacca tgattttttt gaataacttt ccactttttt agcacttcaa ttgtatcatc
      541 gtccagagaa atgatccgca agccgctttc agtttttgtg taaggtttcc gaataaaatc
      601 tttttgattt gtcatttcca aggtgtgatg aaccctcaac tttttacgtt tgaagtccac
      661 gtcattccaa ttaagtgcta atccttcgct tacacgaaca ccagtcatgt aatatagcca
      721 gatcattaaa aaacacatat gttcgtagaa atcatcttta taaatttttg ccaaaacttc
      781 ttcaaattct tcttttgtcc agtaaggaac gattgatttt ccttttggaa ttgcttttgt
      841 tcttttggaa atattctcat tcagaaattg cagggtgacc gcatagtcta atgatttacg
      901 aaacataccg tacataagtg aactgtatgc ttgtgagtat ccgctatcgt tcaataacca
      961 aattcgataa ttttcacaat ctcgcacatt gatttcacga agcttcttat caccgaaaaa
     1021 ttcagtaatc tgaatcaatc cacactcacg agaactccaa gtactttttt gtacgctagc
     1081 tttgtagtgt ggaacgtaag actgctccat aaattctttg taagttaaat cataattcgc
     1141 aaatccattt acaaccatgt attcattttt tatacgcatg acttctttgt gtgcttcacg
     1201 agctgatgag aatcgttttc ctagagaatc ttttgttcct ttcttctgga tcctctttcc
     1261 agtcacttga tctgttccta actcaactag ataagaaaag cgaccatttt tatcttgata
     1321 cacacaaggg tattttgttt tcatagttat tcctcctgtg aaagaggaac acttataatt
     1381 tcttcaatta cttgtcgggg tactacccca actcttttat tattataata tggatatccc
     1441 tgatttacca ttcttgtttt tgcttgtcgg attattgtgg tagcttgatg tgttttaaat
     1501 cctagagcct ctaaatcttt tcgacaaatt gtatttttca ttgtataaac tcactttcaa
     1561 agaaaacact atcattgggt aatagtgtta tttctttatt tttgtataat gaacggtact
     1621 ttcttgaaaa tccatcactt gttcaggact tcccaactca tttaaagcta aagcattgta
     1681 aaaaaatgga cgtctaacta tatattcgct tgtatatagt atatcttgat tcgtggaatc
     1741 atagggtttc tttaccatct ttttggtgac agttggtagt ttgagattcc gggacttgaa
     1801 aaaagctttt ttcttatgct ctttcaattc cagctcttta tcgaaatatt tgctgatata
     1861 gcgcccgcga ttttcgatac tgtctacatc gatcttgttg atcttaacga aaccctgacc
     1921 ccaaatcgta gttaaccggt taaatgagat aaaggggaaa tcaaacaaga taatatggta
     1981 gtggattgca ccgcgttgct gcttttccca cgtggcgaga tactttaaat ttgctttttt
     2041 agttttatag aggtcataat tcagtcgttt gataaatttt ttaaattcat tatttgcata
     2101 ctcaatgtct tgaatgtttt ctttgaaagt gagagttaaa aatttcgttt gattgtcaaa
     2161 attcgtgtct acaaggcggg caatctcaaa tcgcatactt tgataatgtt tctgtttgcg
     2221 ttttaaactg tcatattgtc cttgagcgga taattcatca tagttttttc ttttttcaag
     2281 agtaatatca ttttcataaa tccaagctaa tttttgtttt cgttccagat ttacttcgtt
     2341 ttcgatcgtt ttacgtttgg gagtaatgat tgcttcataa tcataaattt caatatacgt
     2401 gggagtttca atgattttct gattataagc ttgcaaaaat catcactcct tgttgcgtat
     2461 tcgattgtca aagaaccagt cattttctcc tgatgttgat ctataaatca agttaagggg
     2521 gaaggcctgc gtagcagccc ttctagccta tggggtgttg ggcggtctta acggacagaa
     2581 acggctctgt gggcgctgcc ccgccgtttc gtcctaacca cccaacactc catagtatag
     2641 ttagttttgt gtagtggtac cttgttttct gggaatcaaa gttgccgtat cagcggttgc
     2701 cgataaccca taactgacaa attgtccatt cgagcgtccc caaggtacaa tcaccaaatt
     2761 ggtaaatgta attatggcta aaccttgttg ggtaagttct tccggcgtga tcacaggatg
     2821 ttcagatttg actttgacac tgattttttc gtagcgaagt tttggtagga tacattcgta
     2881 gctccaacct agaatttcgc cctccttttg ccagcgagtg acattgacga cttggtattc
     2941 tttttcgagt gtttcttttg gtaaactgag atcggtaatt tttattggca tcacaattct
     3001 ccttttttat acgaaagttt atacgatttg attaaaaaat atagatggcc atctacgact
     3061 tgtattatat acgagaactt atacgtagtc aagagatttt ctaaaaaaat atatacgatt
     3121 ttttatacga gtgttataat gaaaaaaatg ataaggagga atttgaatat gtttggttta
     3181 gtacaaccta ataaagtgaa ggtgggccaa cgaatcaaag aaataaaaga cggaatgtct
     3241 ctctctttta cagaattagg gaatcgatta ggtttaaaaa agccgacgat cagttcctac
     3301 gttcaaggct atgcgttggc tcctaagagt gtgatcaacc aattatctag tatcagcgga
     3361 aaacctgtcg gctggtttta ttttgggcat gtggaagaat atatccgaga gtatttgttt
     3421 ctcattggtc agaaaaaaat agtggacgat tatcctgaaa tcattgagaa aataaagcaa
     3481 gaattttata cgggcgagtt caaaaatcct gcttgggaga atgagctagg ctatcctttg
     3541 gaggaattta tccaagaatg ttttgtagaa tattcgagtg aaatcatgaa ggactatgtc
     3601 gtacagatta cgaaagaggt acttgaagaa tccaaaacgc taaacaaact atcatcaaaa
     3661 gcaaaagaag aagctgtcac gattctttca tctgggatca ttgactatat cgagatgagt
     3721 ggtgagatca agtatggaga gaaagatcgc attgcgaaaa tggtgaaaga atcaataaaa
     3781 acctttgatg tgaaacaaga cctgacattt aatgattcct atttgattgg aaagttgatc
     3841 aatattcttg gtacagatga agatacggag ctgtttcttt cttctttatc aatgatattt
     3901 actgataagc atttttccac tcgatttggt ggggaagaat tagtcgaaat atttcaatcg
     3961 atgcgaccag cgttaatcaa actctatcgg gagaaaacac cagatgaatt ttatgattgg
     4021 tttgagaagt aaaataagaa aagtggctta gattacaagt tggtgaaaaa atttttttca
     4081 gcataacatc agtaatctat aaaggggttg ttgattatac tgtagaagtt aagtactcaa
     4141 gagatttttt gtacgtaaac aaagcaaaaa agggtgcagt ttcactagaa aatgtactct
     4201 tttttgtgat ttttcttagt tatagagttt ttttgaaact ttaaaaatga gaaaacatat
     4261 tgattttaaa gagtgtagac atagcttctt ttgctaaata gattaaactt actggtgagt
     4321 ggtcttgaac atacttttaa atcacatttt actgagtttt attggatttt ataattcatt
     4381 ggaaacctcg tacttaatct gtttttatct atcgactaga atctgtatat attccagcta
     4441 tcaatgaggc cgcagaaaag ggtgcttaaa aataatctgt tgtttaaaat attattatca
     4501 gaatacttag atacaaaatt ctggcacaaa gtaaaaaaaa tattgactta aaaaaatgga
     4561 tatggtaatc tataaacgaa aatagtggta acgtttccaa aatgcatctt cgttaaattt
     4621 ttattttttt aaacgaaaat ggaaacgttt ctaaaatgta ttgggaggag attgaattgg
     4681 aaaagacatt gggaccaggt ggtgagataa gggcggtaca acgggataaa aaaaatattt
     4741 cgaagtggga aaaattaaag aaacagttgc ggcgtgataa gtggatgtac ttgttgctct
     4801 taccaggtat catcatgacg tttatcttta agtatgtgcc aatgtacggt gctgtgattg
     4861 catttaaaga ctataatcct atgctaggaa ttttggaaag tccttgggta ggactagatc
     4921 actttaggga atttatcagt tcaccaaatt ttacgctttt gctgagcaac acattaaaaa
     4981 ttagtattta tggtttattg ttaggttttt ttccaccaat tattttagca ctgagcttga
     5041 atcaaattat caataaaaaa ataaaacaga aaatccagtt gattctatat gcgccgaatt
     5101 ttatctcagt agttattatt gtggggatgc tgtttttgtt cctatccgca aatggaccta
     5161 ttaatgggtt agtagaaaag gttacggggc aaccaattat gttcatgtct aatccagctt
     5221 atttcagaac gatttatatt ttatcgggaa tttggcaagg aatcggctgg tcatccattc
     5281 tttacaccgc aacgctggcg ggagtcagta ccgatttata tgaggcagca aatatcgatg
     5341 gggcaaatat tttacaaaag atttggcata tcgatttgcc tgcattgagg cctgtgatgg
     5401 tgatcagctt catcttggcc gccggcggta ttatgaatgt aggatatgaa aaagcctact
     5461 tgatgcaaac ttcaatgaac atccctgcct cagaaatcat ttccacttat gtatacaaag
     5521 tcggcttgca atcaggtgat tatgcctact ctgctgctgt tggtctgttc aatatgatta
     5581 ttaatgtcgt tttattactg attgtcaaca atataaccaa gcggctaaac gatggtcaag
     5641 gcttgtaatg aaaggagaaa attatgaata gtgctttatc aaaaatcgac cgtcaaatct
     5701 tatggttgaa tcgagtgatt gtaacttttt tagtcgtcat caccattgtt ccgctgattt
     5761 atgtactggt agcttctttt atggacccag ctactttgat caataaaggt ataagcttca
     5821 atccgaaaga ttggacaata cagggatatc aacgggtatt tgcagatgat tccattttac
     5881 gcggttttgt aaattcgttg ttttactctt ttagcttcag cttattgact gtatttatta
     5941 cgatgatcac tgcgtatcct ttgtctcgac ctaacttggc ggggaaaaag attattatga
     6001 ctttttttgt cattacaatg ttcgtgggtg gtggcttgat acctacctat ttattggtaa
     6061 agaacttggg catgttagat agtgtatggg cgattatcgt accaggtgct ataaatgtgt
     6121 ggaatattat cttagcccgt acctatttta gtgctttgcc aaaagaactt tctgaagctg
     6181 ctactatcga tggcgccaat gacttgcaaa tactgttcaa agtcatgctt ccattagcga
     6241 aaccaattat gttcgtattg tttttatatg cctttgttgg gcaatggaac tcatactttg
     6301 atgccatgat ttacatcaaa gatcccgact tacagccgtt gcaactcgta ttaagaaata
     6361 ttttgatcca aaaccaacct tcacaagaca tggtgggagc caatactgcc atggcagaaa
     6421 tgaaacaaat tgctgagttg atcaaatacg caacgatcgt catttctagt ctgccattaa
     6481 tcataatgta tcctttcttc caaaaatatt ttgataaagg tgttttagtt ggttctgtta
     6541 aagggtaagt attgttcact atttgtatga ataaaaatta taaaggagat tttaagatga
     6601 aagcaaaatg gatgctgcca atcgttttta cttccgtact aattttgggg gcttgtggag
     6661 gctcaaaaga aaagagtaaa tcaagccctg attatgaact agaaaatgtc tcttttccat
     6721 tgaaagaaaa agtatcttta aaaatgatgt ctcaaagtgc ccctttggct cccaatgatc
     6781 ctaatgacaa attgattttc caacgattag aagaagagac caatctccat attgattgga
     6841 caaactacag tagtgatttt gccgaaaaaa gaaatttgga tatcgctagt ggtgatttac
     6901 cagatgccat cttcaacgcc gctgcaggtg actatgattt attaaactgg gcggaaagtg
     6961 gcgttattgt accgattgaa gatttgatcg aagattatat gccaaatctg aagaaaattt
     7021 ttgatgaaaa ccccgaatac cgacaaatgt ctactgcacc tgatggtcac atttactcta
     7081 tgccttggat tgaagaatta ggcgaaggaa aagagtcgat tcatacagta aatgggatgg
     7141 cttggatcaa caaggagtgg ttggataatt tgggtcttga aatgccgcaa accactgatc
     7201 agttgatgac tgtgttggag gctttcaaga cgcaagatcc aaatggtaat ggtgaagcag
     7261 atgaaattcc ttttagcttt attaacaatg gaggaaacga agacttaaaa tttttaatgg
     7321 gggcattcgg tctaggtgat aatgatgacc acttagtcgt tgataacgat ggtcaggtac
     7381 aatttactgc taacaaagaa gggtataaag aagccatcaa atatttcaat gaaatgaata
     7441 ataaaggtct tattgataaa gaggctttcg aacaagattg gaatacgtat gttgccaaag
     7501 ggaaagaaca taagtatggc gtgtacttta cttgggataa agcaaatatc acgggtgcag
     7561 atgatagtta tgacgtcttg ccagtcttag cgggaccaga tggaacgaaa catgttactc
     7621 gtacgaatgg catgggtttc tcaagagatc gtttcgtaat tactagtgcg aataaaaact
     7681 tggaacttac agctaggtgg atagataaaa tgtatgaacc gttacagtct gtccaaaata
     7741 actggggaac ctacggtgat gaagcacagg cgaatatttt tgaattaaag gatggtatgt
     7801 taaaacattt gcctttagag gggacagcac cgggggaact aagacaaaaa accgaagctg
     7861 gaggaccatt agcagttctt gattcttatt acggcacagt cactactatg ccagatgatg
     7921 cgaagtggcg tttagatttg atgcatgaca cgtatttgcc atatattaca gaagacaata
     7981 tctatccacg tgtcttttta gaaaaagaag aaacagatcg attagcaaaa atcgaaacgg
     8041 atatgtggga ttacatctat cgcaaaagag cagagtggat caccggcggt aaaatcgatg
     8101 aagaatggga agaatacctt gctgaattgg aacgtgtagg tttggaagaa tggattcaaa
     8161 taaaacaaac tggctatgat aattttttga agggagcaca ataagtgtta gtagaagaaa
     8221 cggcgatttt ggataaagca aatcgattta ttgaaatgat gcgaggaaaa gttaaccctc
     8281 agtatcgaca aaactatcat ttttccgcac ccatcgggtg gataaatgac cccaatgggt
     8341 ttatatatta caaagggcat taccatctct tctatcaata ctatccttac gatagtcgat
     8401 gggggcccat gcattggggg catgcgcgca gtagtgattt ggtccattgg gaagatctac
     8461 cagtagcgtt agccccagat caaccttatg acagcggcgg gtgtttctca ggaagtgcaa
     8521 ttgaaagaga cggaaaacta tatttatttt atacaggctg tcaagaaatt gatggtatta
     8581 gccatcagca tcaatgtttg gcggtctctg aagatggtat tcattttagc aaatacagtg
     8641 gtaatcctgt ggtagcagaa aaacagctag gtgaaaatgg gctaatacaa gaatttcgtg
     8701 atcctaaagt aattgttcac aacgatggtt actatatggt agtagctact aaatctacac
     8761 agtctactgg tagaatagtg atgtttcaat cgtcagattt gtatacttgg cacttttact
     8821 ctgtgctgct agaagggact tcagagcaag gaataatgtg ggaatgtcct gatttattcc
     8881 atttagacgg acgagatgtc ttaatcatgt ctccgataca aatcaagaag aacggactgg
     8941 aatatcataa tatcagttct accatggctt gtattggtga ggtaaactgg gaaacaggtc
     9001 agatgatagt tgataatttc catgaaatgg attttggaat ggatttttat gcaccacaaa
     9061 caacaagaga tcctaatggt ggacgggtga tgattgcttg gatgcagatg tgggatcgta
     9121 cattgcctac acatgagtta ggccatcttt gggcaggctc tatgagtttg ccccgagagt
     9181 taagagtgaa aaaaaatcga ttggtacaaa aacccatctc aactgtatat agtcatttag
     9241 tttatcaaca aggcagtgaa aatattgcag tgactgacac atcagtcgta tttcgaaacg
     9301 cgctgtctga ttcttcatat ttacatctag tcgcagattt ggagaaagct gaagttttta
     9361 caattgaatt actgcggtct aagacggatt gtctcaaact agcctatgac actgctacac
     9421 aggtttttga attggatcgt cagaaactag gaagagagct tacaggggcg gaagaagacc
     9481 ctctgaatgt acgaaaagtt gaagtacctt tgattgataa tcagctcgtt cttgagattt
     9541 ttagagatac ctcatcaatt gaagtcttta ttaatgggtt agaagttatg acggctactt
     9601 tctatgagct ggaaaaagct cgagatatct tgtttcattc gatcggagaa agtcgtattc
     9661 aagcttttga aacagcgagt gtgaaataaa tgtttgattg cttaggcgat gaatcaatta
     9721 tttaataggt tttgaaggat aatgttttta gaaaagaaca gatagctaga tttcccatgg
     9781 atttgctatt agtattttga aaaaattagt aatttatgac aaaattcatt atactaaact
     9841 tgtgcagaaa tgtgagggat caactatgaa agcaacaatg aaagatgtcg caaaattagc
     9901 aggtgttgga gtaggaactg tttcccgggt attgaataac ggttctgtta aagcttctac
     9961 tagagaaaaa gttgaagcag caatgacaga gttaaactat caaccagatt tttatgcccg
    10021 ggggttaaag atgaagcata cgaacacggt tgctttgatt ctacccacgg tatggcatcc
    10081 attttttggc gaatttgcct accatgtaga aacagcatta gaaagacaaa actacaagct
    10141 ttatttgtgt aactccagtg gtaatagtga aaaagaacaa gaatatatcc agatggtcag
    10201 ccaaaataaa gtagacggca tcattggaat tacttattca gatatcgatg aatacatttc
    10261 gagtaatctg ccttttgtta gtattgaccg ttatttcact gaagaggtga tttatgtgac
    10321 cgctgacaat gcggccggtg gacgtttggc tgcaaatgaa ctttatcgcc gaggatgtca
    10381 gaatgtagca tatatcggtg ggtaccaaga aacaccgaat gaaacgaaga gtcgtcgaaa
    10441 atattttgaa atcgagtgcc gtcaactggg gatttcctat aaagcgttag atatgttaga
    10501 acctttagac catctctcag aacgtgtaac aaattttttg ttagagaatc ccgatatcga
    10561 tgggattttt acgatcaacg atccaatggc attagctgtc attgatatgc ttgaaaaaat
    10621 agggaaaaac gtaccagagg atgtccaagt tatcggattt gatggtcaaa agatgacaga
    10681 aggagatcct tatctagtct caacgattgc ccaacctaca aagcaaatgg cggaagtggc
    10741 agtggcaaaa ttaattgctg tcattaatgg agagacagta gaaaaacgta ccgtattgcc
    10801 gattgaattt ggcgaaggtg acacaacgaa acctttagtg agaaacgatt ttgcttagtg
    10861 caaatacgtt tctttttttg aaaatgagtg aatgaaaggt gtagataaaa tgttatatgg
    10921 tggaatagaa gccggtggaa caaagtttgt ctgtgggatt ggtaatgaac aattacaagt
    10981 gatagaacgg aaaagttttc ctaccacaac accagaagaa acgatggagc aagtgtttga
    11041 tttctttgat aagtacaaag gaaatatcgc atcaattggt gtaggagcat ttgggcctat
    11101 tgatattcaa atcgaatcaa gaacttacgg atatatcacc aacacaccga agttagcttg
    11161 gcaaaattat cattttgtag gagctttgga aaacgccttg tcggttccca tctattggac
    11221 taccgatgta aatgctgctt gttatggaga atatatggca gggtatggaa aaggtaaaaa
    11281 tagtgttctg tattacaccg ttggtacagg tgttggtggt ggtgctgtag tggatgggaa
    11341 aatagtcgca ggttttagtc atcctgaaat gggacatatg ctcttacaac gtcataatga
    11401 agatcaattt gaaggaatct gtccttttca tcatgattgt ttggaagggt tagcatcagg
    11461 acctgcgatt gaaaaacgct ttggaagaaa agcccagact atctcatttg atcatcctta
    11521 ttggacgatt gaggcagatt atcttgctca gtgtgtgtat aacacaacgt tactatatgc
    11581 accggaagta ataattttag gcgggggtgt aatgaaacag ccgcaactac tcgagttgat
    11641 tcgccaatcg tttaaagaaa aaatgggttc ctacgtggat acgcctactc tcgaagaata
    11701 tctactaact ccaaggcttg ctgacaatgc gggcttgatt ggctgcctag ctttagcaat
    11761 aaagaaactc aatcattaga atagtattta ttagaactat tcataacata ccggaagttt
    11821 aaaagggtat tctgaaactt aaatagtctc aattcattct tctttcttga gaatctgagt
    11881 agagactttt attctgtttc aaattaatat aattacgaaa tgatattgag tacaagaaag
    11941 ttctttttac cagatctttt aaatcgaaaa atctgatata tatatttata gtactaaaaa
    12001 tctgattaga taaaaagcta attcaatagg aaaataaagg tagtgaataa aagcaaagcc
    12061 cttgttagta gttaatctaa caagggcttt ggtagggaaa atggtaggaa accatcttat
    12121 tttttacgaa aatcaggaac tttagtaatt gaataatgtt gatttatagg tattcctcat
    12181 ccatattatg ttggctcttt attcccactc cactgttgca ggtggtttgc tggtaatatc
    12241 atacacgata cggttaacgt gtgcgacttc atttacgata cggacactga ttttttgcaa
    12301 gacttcccaa gggatacggg caaagtcagc ggtcatgccg tcaatagaag tgacagcacg
    12361 gatacctaca gtgtagtcat acgtacggcc atctcccata accccaacag agcgaatgcc
    12421 tggtaggacg gtgaagtatt gccagatgtc gcgatctaaa ccggcattgg cgatttcttc
    12481 gcgcaagata gcgtctgatt cacggacgat ttctaatttt tcttctgtca attcaccaag
    12541 gacacgaata cctaagcctg gtccagggaa tggttggcgc caaacgatgc tgtcaggcat
    12601 gcctaattgg gtacctaatt cacgtacttc gtctttgaac aatgtgttca atggctcgat
    12661 cagcttgaat tgcatgtctt caggtaatcc acctacattg tgatgtgatt tgatggtttg
    12721 ggcagtttca gttcctgatt cgatcacatc ggtataaagg gttccttgtg caaggaaaga
    12781 aacgccttct tcaccggcaa gtttggttgc ttcatcatcg aagacatata caaattcatt
    12841 tccaatgatt ttacgttttt cttctggatc agacacgcca gcaagttttg ataagaaacg
    12901 ttctttggca tcgactttta taatgttcaa gccaaatttt ccgccaagac tttccataac
    12961 ttgttctgct tcacctttac gcaacaatac gtgatcaacg aagatacatg ttaattgatc
    13021 gccgatcgct ttttgtaaaa gaacaccaac aacagaggaa tctactccgc ctgaaaggcc
    13081 taataaaact tttttgtcac cgacttgttc acgaattttt gcgatctcca tgtcgatgaa
    13141 gttatccatt gtccagtcac cagtacattg gcagacatct agtgtaaaat ggcgcaacaa
    13201 gtcatttcca tattcggaat ggcgaacttc tgcatggaat tggattccgt acattttgcg
    13261 ttctgcattt tggatcgatg cgatggggca atctttgctg gtagcaacga catcgaaatc
    13321 agtcggtgct tgggtaacta ggtcaccatg actcatccaa acggtttgtt tttcaggagt
    13381 gttttggaaa agggccgctt ctgcagaagt gatttccaat tctgcttttc cgtattcacg
    13441 gtttttggct ggtttaactt ttccgcctaa tttataagta atcaactgca taccgtaaca
    13501 gattcctaaa actgggatcc ccaaatcaaa aatcttttca tcaataccaa aggccttctc
    13561 atcataaaca ctgtttggtc cgccagaaag gatgattcct ttagggttca ttgcctttac
    13621 ttcttctgcg gtaatgcggt ggctcatcaa ctctgagaaa acaccgaact cacgaatacg
    13681 gcgagtgatc agttggttat actgactacc gtaatcaagt acgata
//
LOCUS       DAZUMM010000068        13706 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000068 DAZUMM010000000
VERSION     DAZUMM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.070"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..902)
                     /locus_tag="ACG8E6_002032"
     CDS             complement(<1..902)
                     /locus_tag="ACG8E6_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFS5062429.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="ACG8E6_002033"
     CDS             1185..2153
                     /locus_tag="ACG8E6_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5062430.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="ACG8E6_002034"
     CDS             2266..2544
                     /locus_tag="ACG8E6_002034"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5062431.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="ACG8E6_002035"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="ACG8E6_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFS5062432.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3001..3756
                     /locus_tag="ACG8E6_002036"
     CDS             3001..3756
                     /locus_tag="ACG8E6_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5062433.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="ACG8E6_002037"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="ACG8E6_002037"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFS5062434.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="ACG8E6_002038"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="ACG8E6_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFS5062435.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="ACG8E6_002039"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="ACG8E6_002039"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFS5062436.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="ACG8E6_002040"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="ACG8E6_002040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFS5062437.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="ACG8E6_002041"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="ACG8E6_002041"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFS5062438.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="ACG8E6_002042"
     CDS             8493..9035
                     /locus_tag="ACG8E6_002042"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFS5062439.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /gene="atpA"
                     /locus_tag="ACG8E6_002043"
     CDS             9058..10614
                     /gene="atpA"
                     /locus_tag="ACG8E6_002043"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFS5062440.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="ACG8E6_002044"
     CDS             10628..11530
                     /locus_tag="ACG8E6_002044"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFS5062441.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="ACG8E6_002045"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="ACG8E6_002045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFS5062442.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="ACG8E6_002046"
     CDS             12973..13395
                     /locus_tag="ACG8E6_002046"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFS5062443.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4530 a         2452 c         2988 g         3736 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatttggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca
     9901 ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg
    13561 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg
    13681 gaataaggga ctgcgacaag actttt
//
LOCUS       DAZUMM010000069        13700 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000069 DAZUMM010000000
VERSION     DAZUMM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.071"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..344)
                     /locus_tag="ACG8E6_002047"
     CDS             complement(24..344)
                     /locus_tag="ACG8E6_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5062444.1"
                     /translation="MDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLKLLIHT
                     ACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECFFIYDI
                     IASK"
     gene            645..2525
                     /locus_tag="ACG8E6_002048"
     CDS             645..2525
                     /locus_tag="ACG8E6_002048"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5062445.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            2604..2831
                     /locus_tag="ACG8E6_002049"
     CDS             2604..2831
                     /locus_tag="ACG8E6_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFS5062446.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            2836..3378
                     /locus_tag="ACG8E6_002050"
     CDS             2836..3378
                     /locus_tag="ACG8E6_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFS5062447.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            3629..4306
                     /locus_tag="ACG8E6_002051"
     CDS             3629..4306
                     /locus_tag="ACG8E6_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS5062448.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            4648..5868
                     /gene="sstT"
                     /locus_tag="ACG8E6_002052"
     CDS             4648..5868
                     /gene="sstT"
                     /locus_tag="ACG8E6_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFS5062449.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            6015..6650
                     /locus_tag="ACG8E6_002053"
     CDS             6015..6650
                     /locus_tag="ACG8E6_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS5062450.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            6841..7584
                     /gene="truA"
                     /locus_tag="ACG8E6_002054"
     CDS             6841..7584
                     /gene="truA"
                     /locus_tag="ACG8E6_002054"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS5062451.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            7818..8651
                     /gene="msrA"
                     /locus_tag="ACG8E6_002055"
     CDS             7818..8651
                     /gene="msrA"
                     /locus_tag="ACG8E6_002055"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5062452.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            8732..9725
                     /locus_tag="ACG8E6_002056"
                     /pseudo
     CDS             8732..9725
                     /locus_tag="ACG8E6_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            10014..10712
                     /locus_tag="ACG8E6_002057"
     CDS             10014..10712
                     /locus_tag="ACG8E6_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFS5062453.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNIA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNVLAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            10862..11317
                     /locus_tag="ACG8E6_002058"
                     /pseudo
     CDS             10862..11317
                     /locus_tag="ACG8E6_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            11398..12524
                     /locus_tag="ACG8E6_002059"
     CDS             join(11398..11626,11626..12524)
                     /locus_tag="ACG8E6_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5062454.1"
                     /translation="MTRYEENFKQMIIELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIILKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            12569..13525
                     /locus_tag="ACG8E6_002060"
                     /pseudo
     CDS             12569..13525
                     /locus_tag="ACG8E6_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
BASE COUNT         4594 a         2210 c         2822 g         4074 t
ORIGIN      
        1 atactcgttt acataaaaaa agattattta gatgcgataa tatcatagat aaagaaacat
       61 tcatcataag aaacagtgat cccaaattct gtttcaatat ttttgaaggc cttttttaca
      121 tgaaaaattg tttcatgaga aggtcttgtg tcatcttctg atataatttt aagttcatga
      181 ccgtttatca atgtacgctc aattagacac gctgtatgaa tcaaaagttt taaaagaatg
      241 gaatgagtga attttttgtg gaaagtacat tccaattctt ggataaaatc cattaagctt
      301 ttttggatct tattcccagc taataagtag agatatttgt ccactgcttc aactcccata
      361 tcaaaaaacg atatttcttg tgaatgagtg gctgctgccc ttttttgatt gttgttcttc
      421 agtcgtaaga tgacaaagca tcttatcttt gaataaaaaa atagaactac ctaaggaagc
      481 gtttcggcgc ttcctttttc tatatgatcg tttaatgact gtaggaattc agtataccaa
      541 aagaaaaccg cctttttgaa taaacttgat agtggtcaat tttttatgat ggataagtat
      601 ctatacttag tctcgtaaga atgaaaagga aaagagggag aaagatgaaa ttacaaaagt
      661 ggatccaaga gcatcgaaat catatcaccg ccttatcagc tgtgttgatt atttttggtt
      721 ttatcgggaa atttactgga tttgtcttgt tctataacat tgctctagcc atagcttcta
      781 ttattgcggt catacctatt ataatccatg cgtatcaggc agtaaaagtc aaagttatct
      841 caattgattt actcgttact attgctgtgc ttggtgcctt tatgatcgga gaatacaagg
      901 agtcagctat cgttacattc ttgtttttgt ttggtagtta tctggaacaa aaaacattga
      961 ccaaaacgcg ggaatcaatc aaggctttga ccgagatggc cccatcgact gccagcgttg
     1021 tccaagaaga tggaacgacc gaagaagtgg aaatcgatga cgtggaagaa ggcgatatcg
     1081 tcttagtaaa aactggtgga gctattcctg tggacggacg agtcattgac gggaaagggt
     1141 atgcggatga agccgcagtg acgggtgaat cacgacaagt cgtcaaaatc agtggagata
     1201 acgtattttc tggaacgatt ttagcagatg gctacgtgac gatcgaagca actaaggtcg
     1261 gagatgatac gacctttgcc aaaattatag aattagtcga agaagcacaa gataccaaat
     1321 cacatgcgga aaaattcatt gatcaatttt ctcagtatta tactccaacc gttttactta
     1381 ttgcgttgct tgttggtctg atcagtcgtg attttcgttt agccatcact gttttggttc
     1441 ttggttgtcc tggcgcttta gtcattggtg cgcctgtttc gaatgtagca ggaatcggta
     1501 atggagcgaa acggggcatc ttagtcaaag gcggtgaagc aatggatact ttttccaaaa
     1561 tcgatacttt tgtctttgat aaaaccggta cattgaccaa aggaactact gctgtatcca
     1621 ctatcaagaa ctatacagag gatgaagggg acagcttagc tttagtggct cgattagaaa
     1681 cgttatccga tcatcctctg gggcgcgccg tgatccatta tgctcaggag aaagacattg
     1741 attttcaaca attagcagtc aaagataatc agacgatcaa gggacaagga atcacagctg
     1801 agattgatgg tcatacagtt tgtgcgggca acaccaaatt gattgccgct cacaaacttt
     1861 ctctcacacc aaagcaagta caagatttac atcaattaca acaaagaggc agctcagtca
     1921 ttattgtagc aatcgacaaa aaaatcactc aaatcattgg tgtgtcagat gtgattcgac
     1981 cagaagtcgc tgaacaatta gctatgttga aacaagcagg agcaaaacat ttagtgatgc
     2041 tgacaggtga taatcagatg actgctgatt atgtagctga catgttaggc attgatgaag
     2101 tccatgctga attattgcca gatgaaaagg ttcaatttgt gaaaaaatac caagaacaag
     2161 gattgcacgt tgctttcatt ggagatggaa tcaatgattc tccctctttg gctgcggctg
     2221 atatcgggat tgctatggga tcaggaacgg atgtcgctat cgaaacgagt gacgttgtct
     2281 tgatgcaatc tagttttgga tcgttggttc atgcttaccg tttggctaag aaaactgttt
     2341 tgaatacccg tgaaaatgta gtgattgcca ttggtgtcgt cttattttta ctcatcggct
     2401 tatttgccgg gtttatttac atggctagtg ggatgttcgt tcacgaagct agtatcttga
     2461 tcgttatctt taacgcgatg cgtttattgt attttggaca cgaaagcaaa gaagaaaaat
     2521 cgtaaaactt gataagaatc aattttttta tggaagctgt aatctagaat aagactatca
     2581 agaaaaagga aggaagaact acgatgaaaa aaatgattat gcaattagat acactagctt
     2641 gtccaagctg tatgcaaaaa atagaacatg cggtttctca acagcctggg gtaagcaata
     2701 cgaaagtttt gtttaatgcg agcaaaatca aagcaaactt tgatccagat gaagtccacc
     2761 ctgaacaatt agctgcagtt gtggaaaaat taggttatga agtccaatca acaaaagtaa
     2821 aagaggcgta aatcagtgaa tatagtggaa gaaaaatttg ctcaagaaac aaaacaagca
     2881 gatatcgatc atcataaacc aactgctggc gccatggtgg ggcatgtatt ggcaaatttt
     2941 aaattagaag aaatgaaatt gatccaagca caattgtatg ttttgggtac atcttcgcaa
     3001 gaattaagta cccttttttc agaatatgct gatcaagaag cgagatggtt caataaaatc
     3061 tctcatgagt tagtgattga aaatgaaatc gttccgtcta ctttagaaga agtcatgcgt
     3121 tatggaaaaa tttacgaaca tggtcaaaac aaatatttac cagcctcaac aatgttggaa
     3181 aactttgcaa aagattttga tttccaaaat gtctttatca cacgggcaat caaattagct
     3241 gaaaaagaag aaaaatatgg cttgaaacaa ttgcttgttg aacttttaag tttcaataaa
     3301 gaaatcatct accaagtaca aagactccaa ggaaaaactg tccgacaaga tttggatgag
     3361 gaagacgatg atgactaaaa gtctatgttt cttcaaatac aaatataaag cgtgaggtaa
     3421 attggaaaat cgcgaagatt aaaataaaag aagcacccgc tgatgtatta cagacaacat
     3481 cagcgggtgc ttcttagttt ttaaaaggtt taaaagatgg atgagaaaat acaaaaaatc
     3541 aataaaatca cattgcttat ttgatagaat aaagaagtgg gagtaatcga aaggagtttt
     3601 ttaatttaaa aaaaggattt tagtaacaat tggtctagct tttttgttag ctggttgtac
     3661 caacgggaat gaatcgaaaa aagaatcaac aaaagaaaca gcgaaagcaa caattgcaac
     3721 tactacaaca actagcacag agagcaagcc aactgcagca agtacggaaa cagcagatac
     3781 ctctacttcg tcagagtctt ccaaaacgac tgaaacaact caaacatcta cttcaagttc
     3841 ttctgtagag gcttcatctt cagaaagcag cgaacaagtc gaacaaacac tgtggaatgc
     3901 agacaaatcc gcacaattag aggcatttat ggctacatgg ggccaaacaa tgggacaaca
     3961 atacaaaagc tatacgaatc aaatgagtgt ggacttatat ggtcttaaag tccctcaagt
     4021 tattttgaac ggggagtgga agatggctat tggtggagtg cctgtttctg cagaatggtc
     4081 agaaagtggg acaggtcaag cagactatca aatcacagcc gtctattccg atgcagagac
     4141 agaaccatat ctgaaaaagc acgtctatct attcgggttt cagcaaaatc agccaaaagt
     4201 attggtgaca caacaaaatc aggggaatcc cgataattat ctttacttta acgagacagc
     4261 gaataatgaa ttgaaaaatg gctttaatca aatcgtttat gggtaagtaa accagtgaga
     4321 gtgagagaaa gaagagactc agtggaaaat attaattgat tgctatgtct aagaattaag
     4381 atgaaggatg aaatttctag tttcgtcctt catcttagtt acgttttact tggataacta
     4441 taataataac gataaatagt tttctctcga cttctttaga gattgaataa accctccaat
     4501 actaaaattg aatgttgcaa gagcatgaat tgccaattgt attattagaa tttttagaaa
     4561 attgcacctt tagtttcttt agataaaatg atacaatgat tgttaaaatt gtgaaagggt
     4621 ggtcatttgt tagagaagtt tttggatatg tcgttagttc aacgtattgt tgttggtatt
     4681 ttagtcggaa tattgtttgg tatctttttc cccacgtgga catttatttc aatattgggg
     4741 acgttgtttg tcagttgctt gaaggcaatc gcacctattc tggttttttt cttgacgatg
     4801 gcgtcaattg ccaagcataa aattgggaat aagacattcg tgaagcctat tttgatctta
     4861 tatttagtgg gaacattgct ttctgcactt gtagcagtca ttgtaagttc aatttttact
     4921 gttcctatta ctttacaaga aacagtatct gaaaaagcac cgcaaagttt ggaaagtgtt
     4981 ttatctacta tgttgacgaa tgtcacacaa aatcctgttc aatctcttat tgatgcgaat
     5041 taccttggcg ttttattctg ggctgtattg ttgggactag cttttcgagc acgttcagaa
     5101 ccgacaaaag agttggtgga tcagatatct atagcgttgt cacaagtcgt acaagtgatt
     5161 attgcatttg ctccgatagg gattttagga ttggtttatc aatcaattgc tacgactgga
     5221 atcgctggat tggcagagta tctgcagctt ttacttgttt tgatcggtac gatgctgttt
     5281 gtcgcattag ttgtgtaccc attcctcacg tttttgttta ttaaagaaaa tccctatcca
     5341 ttgattttct tttgtttgaa agaaagcgca cttcccgctt tctttacccg aagttctgcc
     5401 gcaaatatcc cgataaacat gatgttggct gaaagattga aattgacaaa agagtcttat
     5461 tccatatcga tcccgttagg tgctacgatc aatatgggcg gtgccgcgat taccatttcc
     5521 ttgatgacgt tgactgctgt acattcttta gggattgaag caccatttgt tttgaaagtc
     5581 cttttatgta ttttgtctgc tttagctgct tgtggagctt caggtgttgc gggcggttca
     5641 ctgttattga ttccattagc atgtagtttg tttggtatct ctaatgatgt cgcgatgcaa
     5701 atcgttggta ttggatttat tatcggtgtt atccaggatt caatggaaac tgccttaaat
     5761 tcgtcaagtg atctgttgtt tactgcaata ggagagctag gcgcgcgtaa aagaagtggg
     5821 gaaaaaatca aattgaaaga ttgtttagat ggattaagtg aacgttgaac gttagatatt
     5881 tagtattttt aaaatgattc gagtttttaa tgagttatat agatgttcgt gtaaatagag
     5941 gtttctgtta aatcaagata aagtcgttta taataacaat taaaagcata ggtaattgtt
     6001 attggaggcg tatcatggat tttttacgta aatttggttt tttcttttta ttattaggcg
     6061 tgctgagtga ttttttgact ccgtacgtat taggattgta ttatcctgaa ttagatcaaa
     6121 tgaagagcgt gatcagtgta tttggagagg tttccagtcc tgtaagaaaa gcgtttctta
     6181 tttggtctgt ggtttcaggg atctttttca tcctgtcatt gcctgcattg tatgctgcat
     6241 ttgtaagtac atctcgtgtg ttagctaccc tagttacagc agccatcgcg ttctatggta
     6301 taggtgattg tatctttaca ggtttgttca gtatcaatac ccatgaatct tcgtggaatg
     6361 tctcgacatg ggtccataat atcggatcag gtttaggtta cagtggtttt ttattgtttc
     6421 cagttcttct tttcttgatc tatcgaaaaa atgggcaaat ggacgtaagc cacttatacc
     6481 ttggactgtc tgtgatcagt ttgctttttg cggcgattta tggactggca agaattcctt
     6541 ctataaattc ttggccatta ctgaataaaa taggtttttg ccaaagaatc agttttttct
     6601 ttaattatgt gcccattgcc tggttgagta tcttgcatct caaacagtaa aacttgagcc
     6661 gtcaaattga acagtcagcg gatacttcat ccggtggctg ttttttgttt ttctgaaata
     6721 tatatttcca cagttttctt gtatagcttc tgtcttagag aagcaaggag ctatattttg
     6781 tgctacaatc ttaaaaaggt agagagaaga ccaaatggaa gacagaggag ccactaaaat
     6841 atgagaaaca tcaagttgac aatcgaatat gacggaaaaa gatattcagg atggcaaaga
     6901 cttggagatg acgataaaac cattcaagga aaaatcgaaa aagtattgca tcagatgaca
     6961 aatgaagaga tagaaattat tggatcagga agaacagatg ctggcaccca tgcgcgagga
     7021 caagtagcaa attttaaaac aaacacagaa attggactgg cagaaatgat cgactttatg
     7081 aatcgttacc ttccgcggga tattgtcatc aaaagaatcg aagaaatgcc tgagcgcttc
     7141 cattcaagat ataatgcggt gggcaaaaaa tacagctatt atgtttggaa caacgtgatt
     7201 ccttcagcat ttgagcgaaa ccacagcttt tatttccctc aagagttgga tatggacaag
     7261 ctgaatgcag cttgcgaaaa attagtgggt acacatgatt tcattggttt ttctgcattg
     7321 aaaaaatcaa aaaaatccac agtgcgtacg attgatgaat tatctatcga acgaaaggga
     7381 gaaatgcttc attttacttt tgtcggagac ggattcttat ataaaatggt gcggattatc
     7441 atggggacat tactagaaat cggcacaggt gccatgccga tagaaacaat cgatgaaata
     7501 tttgaaagca aagtgcggca acatgcagga gaaaccgtcc ctgcacaagg tctgtttttg
     7561 gatgaagttt attatcgctc ataaaaaaga atcatcttta ctcgacttct gagaattgga
     7621 gaacatccct tttttcagag gtcgtttttt agttaaacca aaccatagag tatgcttttt
     7681 aagataaaga tcatcttata tcatctgaag ctgcttatct tctttgaata aatacgattc
     7741 aatcgattat ttcctcactt tatgataaat tacagctaaa gtcactagct tgtcttcttc
     7801 ggaaaggagg agaatctatg gatgaggaac aaatattcat tcaaacactt tataatctga
     7861 ttttgaatcc gaatacacga gattgggaac gaaaagtatt gattcagaca aagaatgaca
     7921 caagagaaaa tatatccgtt aaagaacagt tatcaaaact agaagtaaca ctacgtccat
     7981 tagctatccg aatgaatctg acgcctgatg taatggactt ttatttattg attacggaag
     8041 gatttgataa ggagcaaaaa tatgactttt ccaagcatgc catgcaagat gctgactatc
     8101 aagaacgtgc agtatttgca gggggatgtt tctggtgtat ggtcgagccg tttgaatcga
     8161 aaaaagggat cctatctgtt ttgtcaggat acacgggcgg acatgttgaa aaaccgaatt
     8221 atgatcaagt aagtggtggt tacacaggtc atgtggaagc tgtggagatc atttatgaca
     8281 caagagaaat aagttatagc gagttgctga caatctattg gcaaattact gatcccacag
     8341 atacatttgg acaatttcaa gatagaggga agcaatatcg tccagtgatt ttttatcaag
     8401 acgaacgaca aaaagaatta gcggaacaat caaaacagaa actagacagt tcaggaactt
     8461 tccaccaacc aatcgtcaca aaaatcgaac cagccggtac gttttggcca gcagaaaact
     8521 atcatcagca attttacaaa aaacaaccga aacgctataa aaaaatccag caagcacgta
     8581 atcaatttct gatctatcaa cgagtaaaaa acaaatggca aaaaaatata cgaaaaaatc
     8641 attttgatta actccttttc tttaaaatgt agtaaactaa aaatagaaaa caagaaagcg
     8701 ttttaaaaag atttgatgag gaggagaaaa attgtatatt gcagatagtg cacgttatga
     8761 aaaaatggaa tatcgtaaat ccggtcattc aggtttaaaa ttaccgattt tatctctcct
     8821 ctcggattat ggcaaaattt tggtgattat gatcctatcc ataatcaaag agagatttta
     8881 agaggcgcat tcgacatggg aattacccat ttcgaccttg cgaataatta tggaggccct
     8941 gctggagctg cagagaaaaa ctttgggcgt atttttagag aagattttca agcctaccgt
     9001 gatgaactga ttatttctag caaagcaggt tatcatatgt gggaagggcc atatggggaa
     9061 tggggctctc gtaaatccat catatcaagt tgtgaccaaa gtttccaaag aatggggcta
     9121 gattatgttg acatcttcta tcatcatcgc ccagaccctg atacaccttt ggaagaaaca
     9181 gcagaagcgt tgatgcaact tgtaagacaa ggaaaagcat tatatattgg tatatccaac
     9241 tataatggag aagatacaaa aaaatgactg aaattttaaa aagaaaggaa gcaccgttca
     9301 ttattcatca gatgcgctac aatatgtttt ctcgtgcact actagaagac gatctatctc
     9361 cagtattaga agaagaaggg ctaggggcaa tcacgttcag tccgcttgct caagggttgc
     9421 tgacaaatcg ttatctacat ggtattcctg aagattctag agcacatcgt aaggaaatac
     9481 cattcctttc agaagaacaa gttggatcaa ctctggaaaa aatcaaagca ctgcaaacaa
     9541 tcgccgtatc acgaggacaa tcattagcgc aaatagccct tgcatggaat ttacgacaaa
     9601 aatctgttac aagtgtactg atcggagcaa gtcggttaag tcagttacaa gaaagtgttc
     9661 gaatgatgga taatttagat ttctcaccag aagaagaatt gagaatcgac caaattctag
     9721 aatagaaacg atgtaatcaa actttttatc catatattaa aaaaaacaaa agatgacatc
     9781 aagaatcgaa tgaagatcgt ctgttattga acatcatatt ttttgtcgat caaaataaaa
     9841 atgatcgaag gctaggagtg aattttatcc tagtcttttt tatggtaaat atcttcttcg
     9901 tttgtatgtc tatgaacgga ttaatcgatt ttttatacaa aaaatcatca gatgattcaa
     9961 acgtgttata ataattacga aaaaatacat cagatgaaaa ggaggtatgt ggtttggaag
    10021 aaaataaaac cctagtagaa gtaactactg acaagatcct tagatattta aaggaaatgc
    10081 agttgcaagt aggagataaa ctgccgaatg aatatgaatt gtccgattat ttagaggtgg
    10141 ggcgaagcac ggtgcgagaa gctgtgcggg ctctagcttc caggaatatc cttgaagttc
    10201 ggcaaggagc ggggacattt gtcagtgata agcgagggat tgccaatgac ccactcggtt
    10261 tttctttgat tgaagatcag gatcggatga tacgagactt atttgaactg agatatttat
    10321 tggagcctaa gatggcaggt ttggcagcgg aatatgcaac gaagaatcaa atcgtccaaa
    10381 tgaaaaaatt aacgcaaaag attgaaactt cttttcgaga aggcacacaa gaacatgttc
    10441 aattagacat cgattttcat acgatgattg cagaagctag cggaaacata gccttgctgc
    10501 atattatccc aatcattaat gaatcgattg ctttgtttaa taaaaattat gatatccagc
    10561 atctgaaaat cgagacgatc gatattcata cagagatcgt tcaggccatc gaaaggcgga
    10621 acgtacttgc tgcgtttgac gcaatgactg tccatatggc aataaatcgg agagaattac
    10681 agaaaataat aggagaaaaa gaaactaatt gacagttcat ctgatgtatt ttatgataaa
    10741 tacatcagat gattttttgt tttatatttg gtaaaaaaga ggagtaaata gtttctgttc
    10801 accaaaatgt aagcgctgac taatagacaa aaagagatga cgtcaaggag aagaagcagg
    10861 aatgagggga tctggaatga gttttatgga taaattagcc aaagttttag ggaacgtggc
    10921 aacgagaatc aataatttaa ggtatatcat ggtgataaaa aacgcatttg ctgcattgat
    10981 tccggtaatt attacgggtg cttttggcac attgttttca gcaatggtct ttgactcgga
    11041 aaatggctta gcacagataa gttacttgcg ttttttagaa gaattaaagc ctatcgcatc
    11101 tgccatcagc tatgtcacac taagtttctt gaccatctat gctgtttttc taatagggat
    11161 cgaattagcc aagctgaata aagtggatgg tgtattccct ggtatcatcg cagtgatgtc
    11221 ttatttatct gttaatccaa ctgtttatga attagtgaat gaaaatcaga ctgttatagt
    11281 agaaaacgta ttggctaaac aatatacaga cacgaaagtg gtgttagtat caaatcttag
    11341 acagcttttc gtagagacaa gaataaatta aacttatcta cgagaggatg aatttttatg
    11401 acccgatatg aagaaaattt taaacaaatg attattgaac tgaatcaaac tggacgttct
    11461 gttcgagggt tagcgaaaga atatggctta tctgaagcaa cgatttacaa atggaagaat
    11521 ttatatttac ctgatcagtc cacaggactg actggaaaag aagtagctga actgagaaaa
    11581 gaaaatgctc gtttaaatga ggaacttgaa atcttaaaaa aagccgcagc catattctct
    11641 cggaaaacct aaattcgctt gttcaattta ttgaaaaatg gtgtaaggat tacactgttt
    11701 ctttgttgtg tcggttatta gaaatcccta gaagtgtcta ttatttttat aagaataaac
    11761 cgttgacagc tacagaaatc agaaataata agttgaaaaa gaagatttca acaatttttt
    11821 ttacgaataa acaacgctat ggtgccacaa agatccatca agttttatta aaagaaggga
    11881 tttcagtatc tcttaaacat gtccaaaagc taatgaaaca attaaattta aggtcgattg
    11941 tagttaaaaa atatagacct caaaggtcta ataaaccgat cattttaaaa gagaatctct
    12001 taaatcagga cttttctact gaaacgatct gtgagaaatg ggcagctgac attacgtaca
    12061 ttcctactaa gaaaaatggt tggtgttact tatcttcaat catggattta cacacgaaaa
    12121 aaattattag ttatacattt tcaaaacgaa tgactgtgga ttgtgtcatt caaacattga
    12181 ataaagcaaa aatacattat cacattccag aaggaatgat tctacacact gacttgggca
    12241 gccaatacac agcaagagaa gtggaacaat ggcttaaaac caacaaaata aggcattctt
    12301 atagtagaaa aggaacacct tatgataatg ctggaatcga atctttccat gcctcattga
    12361 aaaaagaaga agtctacacg actagctact cagattttga agaagcaaat cgagcgctat
    12421 ttagctacat tgaaggattt tataaccgaa atcgaatcca tagctcgatt cactatctaa
    12481 ccccacagga atttgaagaa ttagcaaaag aaaaaatggc ttaaccgtct ctactaaaat
    12541 gtgtccaaga tattgactca aatccattga tcgttacggt gatagggtaa aaaactggtt
    12601 tacattcaac gaaccaatcg ttccccaaac gcgttgctat ttggatgctg ttcgctggcc
    12661 gcatgaacaa aacacaaaaa agtggatgat gtggaattat cataaagttt tagcaacggc
    12721 taaagcagta gagatttacc gcagcggaaa ttatggtgga aggattggct gtattttaaa
    12781 tcctgaaatg gtctatgctc gatctaattc tccagaagac caaaaagctg cttatatgta
    12841 tgatttgttt tataatcgag tattttttga tccgatggta aaagggtact acacagaaga
    12901 gttcctggag ttgtgtcaaa aacaatcgat ttatttcgat cctgcaacag aggatttaga
    12961 aacgattcag catcatacag tagatttcct tggaatcaat caatattatc ctaaacgcgt
    13021 tcgcgctcct cgttatcaat ggaatgaaga gacacctttc catccagaac gatattatga
    13081 agtctttgat ttacctggga agaaaatgaa taattccagg ggatgggaaa tctatccgaa
    13141 aatcatgtat gatatagcca tgtatctaaa agaaaattat cataatatcc cttggctgat
    13201 aacagaaaac ggtatgggaa gagaacatga agaacggtat atggatgaga aaggaatcgt
    13261 gcaagatgat taccgtatcg aatttatcgg acagcatttg cgttatttga tcaaagcaac
    13321 agaagaaggc gcaaattgcg aaggctatat gttatgggca ttcactgact gtgtgtcacc
    13381 aatgaatgca tttaagaatc gatatggatt agttcgaatc gaattgaacg atgaacgaag
    13441 cagaacattg aaaaaatctg cttcgtggta tcagactttg attgataaga gagaattgga
    13501 tttgcctgac gaaatctata aataaataga agttttttgt agatgttcat tgtatttact
    13561 ggttatttga aataaaagag gttgtgaaaa agctgtcctg catcaagtaa taagaatagc
    13621 aaagtaaaaa caacatctta tgtttttaac ttggttggac ttattaccgc agatgcaagc
    13681 ttttgaatac cgtttattcg
//
LOCUS       DAZUMM010000070        13581 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000070 DAZUMM010000000
VERSION     DAZUMM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.072"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..250
                     /gene="yaaA"
                     /locus_tag="ACG8E6_002061"
     CDS             8..250
                     /gene="yaaA"
                     /locus_tag="ACG8E6_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFS5062455.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            237..1361
                     /gene="recF"
                     /locus_tag="ACG8E6_002062"
     CDS             237..1361
                     /gene="recF"
                     /locus_tag="ACG8E6_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFS5062456.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1358..3304
                     /gene="gyrB"
                     /locus_tag="ACG8E6_002063"
     CDS             1358..3304
                     /gene="gyrB"
                     /locus_tag="ACG8E6_002063"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFS5062457.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3324..5795
                     /gene="gyrA"
                     /locus_tag="ACG8E6_002064"
     CDS             3324..5795
                     /gene="gyrA"
                     /locus_tag="ACG8E6_002064"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFS5062458.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6031..6330
                     /gene="rpsF"
                     /locus_tag="ACG8E6_002065"
     CDS             6031..6330
                     /gene="rpsF"
                     /locus_tag="ACG8E6_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFS5062459.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6380..6892
                     /gene="ssb"
                     /locus_tag="ACG8E6_002066"
     CDS             6380..6892
                     /gene="ssb"
                     /locus_tag="ACG8E6_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS5062460.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6918..7154
                     /gene="rpsR"
                     /locus_tag="ACG8E6_002067"
     CDS             6918..7154
                     /gene="rpsR"
                     /locus_tag="ACG8E6_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFS5062461.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7297..9270
                     /locus_tag="ACG8E6_002068"
     CDS             7297..9270
                     /locus_tag="ACG8E6_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFS5062462.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9276..9728
                     /gene="rplI"
                     /locus_tag="ACG8E6_002069"
     CDS             9276..9728
                     /gene="rplI"
                     /locus_tag="ACG8E6_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFS5062463.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10002..11369
                     /gene="dnaB"
                     /locus_tag="ACG8E6_002070"
     CDS             10002..11369
                     /gene="dnaB"
                     /locus_tag="ACG8E6_002070"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS5062464.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12149..13441
                     /locus_tag="ACG8E6_002071"
     CDS             12149..13441
                     /locus_tag="ACG8E6_002071"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFS5062465.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4520 a         2465 c         2989 g         3607 t
ORIGIN      
        1 atgggcattg aaacaacaag tcattctaaa tacggaattc atgacgttgg gccaaatgtt
       61 gaaagaagtc acagttatcg gtagtggcgg acaagcaaaa tggtatttgg cagaaaacac
      121 tgtgttagtc gatggcgaac cagaaaatcg tcgtggacgc aaactttatc ccggcatgat
      181 ggttgaagtt ccagaagtag gaactttttt tatggtaaaa aaaggaaacg acgataatga
      241 ggctgaataa gctgtattta aaaaattatc gaaattacga agagctgaac atcgaatttt
      301 ccaagaacct cgtcatcttt ttaggagaaa atgcgcaagg aaaaacgaat cttttggaaa
      361 gtatctacgt attagctatg acgcgaagtc atcggacaag tagtgagcag gaattgattg
      421 gctggactga cgatcaggcg atgattcaag gagagatcac aaaaggttca tccactcttc
      481 cattagaaat cttattatct aaaaaaggcc gaaaaaccaa gatcaaccat atcgaacaga
      541 aaaagttaag tacttacgtg ggacaaatga atgtcatttt atttgcacca gaagatcttt
      601 ctttagtaaa aggcagcccg cagaccaggc ggaagttttt agatatggaa ttaggccaga
      661 tcaatccaat ctatctttat gatcttgtcc aataccaagc aattttgaaa caaagaaacc
      721 agtacttgaa gcaattgaat gaaaaaaaac aaactgattt gctttatttg gatattttgt
      781 ccgaacaact agcagcgttt ggcagcaaag tattgaaagc aagagcacag ttcgtcaagc
      841 gattggagtt ttgggctaat tctttgcatc agcaaatcac ccatcaaaaa gagcagctgg
      901 aaatcgagta tctcaccgca gtcgattctt tagaaacaca tacgcaagag caaatccaag
      961 aacaatttct cgcacttttg aaccaaaata aaaagaaaga cctttttcgg ggaacaacga
     1021 cagttggtcc tcatcgtgac gatctttcct tttttattaa tcagaaaaat gtgcagactt
     1081 atggatcgca aggacagcaa agaacaactg cactaagtgt caaattagca gaaatcgatt
     1141 tgattaaaga agagacaggg gaatatccaa tccttttgtt agatgatgtg atgagcgagc
     1201 tggatgataa tcgtcaactc catctgctag aaacaatcga aggaaaagtt caaacgttct
     1261 taacaacaac gacacttgac catgtaaaaa ataaaatgac tgttgaacct gagatttttt
     1321 atgttcaaca agggaaacta aaaggagaag aaactgaatg acagaagaaa gaagtttagt
     1381 agaacgcgct aaagagtatg atgccagtca gattcaagta cttgaaggac ttgaagccgt
     1441 tcgtaaacga cctggcatgt atatcggttc gactagttct gagggtctgc atcaccttgt
     1501 ttgggaaatt gtcgataatt cgatcgatga agtattagca ggttttgcaa cgaaaatcca
     1561 tgtgattatt gaaaaagaca acagtatcac cgttattgac gacggtcggg ggatacctgt
     1621 agatatccag gcaaaaactg gacgtcctgc agttgaaacg gtctttactg ttttacatgc
     1681 tggaggaaaa ttcggcggtg gtgggtacaa agtttctggt ggacttcacg gagtaggatc
     1741 ttctgttgtt aacgcgcttt ctacacagct tgatgtcaaa gtatacaaag aaggaaatgt
     1801 gtactatcaa gaatatcgtc gtggagctgt agtggacgac ttaaagatta tcgaacaaac
     1861 ggatcgccat ggaacaacgg ttcactttac gccagaccct gaaattttta cagagacaac
     1921 tgagtttgat ttttcaaaat tggctacccg gatccgtgaa cttgctttct tgaaccgcgg
     1981 gatgcgtata tcgattgaag acaaacgaga agaagaacca gtcatcaatg aataccatta
     2041 tgacggcggg attaaaagtt atgtcgaata cttgaatgca aacaaaaccg tgatttttcc
     2101 agaacctgta tatttggaag gtgaacagca agatatcgca gttgaagtct ctatgcaata
     2161 tacagacggc taccattcca atatcatgag ttttgccaac aatatccata cgtatgaagg
     2221 cggaacacat gaatcaggat tcaagactgc tttaactcgt gtgataaatg attatgcccg
     2281 aaaacaaaag ctgatgaaag aaaatgacga caacttgacc ggtgaagacg tgcgagaagg
     2341 attgacagct gttatttcta tcaagcatcc tgaccctcaa ttcgaaggac aaacaaaaac
     2401 aaaattgggc aattccgaag tacggacagt cactgatcgg ttatttagcg aacatttcat
     2461 gaaattcttg ctggaaaacc caagtgtggg ccgtcaaatt gttgaaaaag gattactagc
     2521 ttccagagct cgtctagcag cgaaacgtgc gcgtgaagta acgagacgta aaggtgcttt
     2581 agagattagt aatctgccag gaaaattagc ggactgctct agcaacgatc ctgaaaaatg
     2641 cgaattgttc atcgtcgaag gagattcggc cggcggatca gctaagcaag gtcgtaatcg
     2701 agagtttcaa gccatcttgc ctattcgtgg gaaaatcttg aatgttgaaa aagcaagcat
     2761 ggacaagatc ttggcaaatg aagagatccg gtctttattt actgctatgg gtacagggtt
     2821 tggagaagat tttgatgtat cgaaagctcg ctaccacaaa ttagttatca tgactgatgc
     2881 cgatgtcgac ggcgcgcata tccgtacatt gctgctgact cttttttatc gttacatgcg
     2941 tcctattgtt gaagctggat acgtctatat tgctcagcca cctttatacg gggtaaaaca
     3001 agggaagaat attacgtatg tacaaccagg aaaaaatgca gaagaagagt tgaaacaagt
     3061 cgttgcttca cttcctgcct caccaaaacc aagcgttcag cgttataaag gacttggaga
     3121 aatggatgat catcaactat gggaaacaac tatggaccca agcaatcgga tgatggcccg
     3181 cgtcagtgtg gacgatgcta ttgcagcaga tcagatattt gaaatgttga tgggggatcg
     3241 agtagaaccc cgtcgtgcat tcatcgagga aaatgcacac tatgtgaaga acttagatat
     3301 ttaatcatga aggaggaaaa agaatgagtg aagaaatcag agaaaacatc catgatgtca
     3361 atctaaccag cgaaatgaaa gactccttca tcgattatgc gatgagtgtt attgtcgcac
     3421 gtgcacttcc tgatgtcaga gatggtctga aacccgttca tcgccgtatt ttatacggca
     3481 tgaacgaatt aggtgtaaca ccggataaag cccataaaaa atctgccaga atcgtcggag
     3541 atgtcatggg taaataccat cctcatggag actatgcgat ctatgagtca atggtacgga
     3601 tggcacagcc attcagctac cgttatatgc tagtggatgg tcatggaaac tttggttctg
     3661 ttgacggtga cggtgccgcc gctatgcgtt ataccgaagc acggatgagc aaaatcgcta
     3721 cagaaatgct gcgtgatatc aataagaaca ctgttgattt ccaaagcaac tatgatgata
     3781 cagaaaaaga accagttgta ctacctgctc gcttcccgaa tcttttagtg aacggaacaa
     3841 cggggatcgc cgtaggtatg gccacgaata tcccacctca taacttgtct gaggtagtag
     3901 cggctatcga tttgctgatg gataatcctg atgtgacgac caatgaattg atggaagtat
     3961 tacctggacc agattttcca acaggtggtt tggttatggg aaaatcagga atccgaagag
     4021 cgtatgaaac aggtaagggt tctattaccg tacgtgcaaa agttgagatt actgagatgc
     4081 caaatggtaa agaacggatc atcgtgacag aattgcctta tatggtcaat aaagcaaaat
     4141 tgatcgaacg gatttctgaa ctgcacagag acaagcgtat cgaagggatc acagatttgc
     4201 gggacgaatc ttcccgtgaa ggtatgcgga tcgtcattga catccgtcgt gacgccagcg
     4261 catctgttat cttgaataac ttatataaat tgacatcctt gcaaaattca tttggattca
     4321 atatgttggc gatcgaaaaa ggtgtgccga aagttttgag cttgaaacaa attcttgaaa
     4381 actatatcga acatcagcgc gaagtcatca caagacggac agtctttgaa aaagaaaaag
     4441 cagaagcaag ggcacacatt ttagaaggtt tgcgtattgc tttggatcat atcgatgaaa
     4501 tcattgcgat tatccgcggg tctaaatcag atgacgaagc aaaagcgacg atgatcgaac
     4561 ggttcgattt atccgatcgc cagtctcaag ctattttaga tatgcgttta cgccgattga
     4621 ccggtttgga acgggaaaaa atcgaaaatg aataccaaga actcttaaaa ctgattgaag
     4681 atttaactga cattctggca agaccagaac gtgttacaga aatcatcaag aatgaattag
     4741 cagacttgaa tcaacgattt ggtgataaac gacgcacaga attactagtc ggtgaggtct
     4801 taagtctgga agatgaagat ttaatcgaag aagaagaaat cgttattaca ttgacaaaca
     4861 atggttacat caaacgtttg gcaaataatg aattcagagc tcaacgaagg ggtggacgtg
     4921 gtgtccaagg aatgggtatc cacaatgacg acttcgtcaa aaatctggtt tcttgttcta
     4981 cccatgatac attgttattc tttacgaata acggaaaagt gtatcgggca aaaggatacg
     5041 aaattccaga atacgggcgc acagccaaag gaataccaat tatcaattta ctaggtatcg
     5101 attcgagtga aagcatccag gcaatcattg cggtgacggg agaagcagaa gaaggccact
     5161 atctcttctt tacgacgcgt caaggtacag taaaacgtac ttctgtcact gcttttgcta
     5221 acatccgaag taatggtctg atcgcaattg gtttgaaaga agacgatgag ctcgtcaatg
     5281 tgctactgac agatggtcaa tctaatatca ttattggaac acataacggc tactctgtca
     5341 ctttcaaaga agaagcagtc cgcgatatgg gacgtacagc ttccggggtt agaggaatac
     5401 gcctgcgtga ggaagattat gtcgttgggg cagccttaat gaaggaagat caagaagtat
     5461 tagtgatcac agaaaaaggt tacggaaaac ggacaaaagt atcggaatat ccagtgaaag
     5521 gccgcggagg taaagggatc aaaacggcga acatcactga aaaaaatggt ccactagctg
     5581 gattgaccac tgtttctgga gatgaagaca tcatgctgat tacagataaa ggtgtcatta
     5641 tccgtttcaa cgtatcgact gtttctcaaa ctggacgttc aacattaggc gtacgtttga
     5701 tgaagatgga agctgatacg aaagttgtca cgatggcagc agtcgaacca gaagctgcag
     5761 aacaaacagt tgaagaacaa ggatcagaag aatagccatc aagactaatt gtgtaagcta
     5821 gcaagaatga ccgattaaaa aatctggtta ttcctgctgg cttttttgat tagttgaatt
     5881 tctcttaaaa tattcgaaac tgttcattct gtattgcttt tgaccaaaat agagtgtata
     5941 atcagagctt gtgagtagtc taattaatta gataactctc cttgctctta ggaacctaag
     6001 agccgaaagt ccacagggag gtgaatatca atggaaaaca cgaaatatga aatcatgtac
     6061 attattcgtc caaacattga tgaagaagca aaaactgctt taatcgaacg tttcgatgca
     6121 atcttgaaag ataacggagc agaagttatc gaatcaaaag attgggaaaa acgccgtttc
     6181 gcttatgaaa tgaacggtta ccgtgaaggt atctatcaca tcgttaaagt atcttctcca
     6241 tcatcagcaa atgctgtcaa tgaatttgac cgtcttgcta aaatcaacga cgatatcatt
     6301 cgtcacatga ttgttaaaga agaagcttaa ttgttgtttc atgtgaaaca atagaaaacg
     6361 atgagaggag atgactattt tgattaataa tgttgtatta gttggacgtc tgacgaaaga
     6421 tccagatttg cgttacacag caagtggtac ggcagtcgca acatttactt tggctgtgaa
     6481 tcgtaatttt acaaaccaaa gcggcaatcg cgaggctgat tttatcaact gtgtgatttg
     6541 gcgcaaatca gcagaaactt tagcaaacta tgctcgtaaa ggaacacttt taggtgttac
     6601 tggacgtatc caaactcgtt cttatgataa tcagcaagga caacgtgtat acgtgacaga
     6661 agtagtagct gacaacttcc aattgctgga aagtcgttct gcatctgaaa atcgccaaca
     6721 aagcggcgga ttccaaagtt ctggtcaatc agctggcgga tttggcggaa acaataactc
     6781 taatcaaaca tctcaatcat ccaacggtat gcctgatttc gaccgtgata cttctgaccc
     6841 attcggctca agttcaacga tcgacatttc agacgatgat ttaccattct aaacgaattt
     6901 gataggaggg aaatagaatg gcacaacaaa gaagaggcgg acgtaaacgt cgtaaagtag
     6961 actacattgc tgctaaccat attgaatata tcgattataa agatatcgaa ttactaaaaa
     7021 gatttatttc agaacgcggt aaaatcttgc cacgtcgtgt aactggtaca ggcgctaaaa
     7081 accaacgtaa aattactgtt gcaatcaaac gcgctcgtat catgggcttg ctacctttcg
     7141 ttagcgaaga ctaataaatc ctaggaattg aatgactgat ccttttgatc agctattcaa
     7201 ttcctttttt tctgtaaaaa taacctctat tcgtttagtc gctcgaatga cgaaagttat
     7261 ggtaaaatca aattagtcat atatgggagg aaaattatgg aaaataaaaa caatcattta
     7321 tccccgaaac ctctcttcct tatcctgttt atattaatcg aagtattggt agtcttaatt
     7381 ttaccatttc gttttgttag cgcttcggtc attctattga tgaatttatg gggtgttcgc
     7441 tattggttga aaatgaaacg ttggcttatt ttaaataaac gtgaaaaaat ccagcaggct
     7501 tcgactgtag ctgaaaccaa tctagcttat gtctcagaag tcatgcctgt aggagtgatt
     7561 gcttatcagc catcaagcca aaaaatcgaa tggctcaatc cattagcatt gaaaatcaaa
     7621 gatcaaagtg gattaacaga ggaagaattg ctagatactt tttttactgc aaaagaaaaa
     7681 aagaaaaacc aagtctatct agcagataaa acatttcagt ttgatatgga tacagtaaag
     7741 ggtgttgttt attttaatga tattacccaa aaccagttgt taaaactcca tcagacgaac
     7801 agtcagccgg tgattggtat tttatctatt gataactatg cagatgccat tgataaattg
     7861 gatgacaaag agatttctta tttgaactct ttcgtcacta cactagtttc tgactggatg
     7921 aatgaatacc atgtcttttt caaaagaatc aacgcagaac ggtttttctt cgtagcacag
     7981 atgatcgatc tgaaaaagat gatggataaa aaatttgatt tgctggatcg ctttcgagca
     8041 gaagctgaaa aacagagctc gcctatcacc cttagtatgg ggattgctta tggctcaaga
     8101 accctcgatg aaatcggtgg agaagctcag aataatttag atgttgcttt agtccgtgga
     8161 ggcgaccaag ttgtcgtgaa agaggtaaaa gaaggagcaa aacctgttta ttatgggggg
     8221 aaatctgcta agaatgtcaa acgcacgcgt gttcgttctc gagcgatgag taccgctttg
     8281 cgtcggattt ttgatgaaac aggcgatgtg tttatcatgg ggcacaagtt tccggatatg
     8341 gatgctttag gctctgcttt tggtgtcagt tgtctggcac gcttcaacca gaaaaaagct
     8401 tacatcgtta tcaacgagaa cgagatcatt ccagatgtag aacggtgtct tgaagaaatc
     8461 cataagcatc ctgaattaga aatgcaacta atctctccag aacgtgccat agagctgaaa
     8521 aaagaagagg acctcctcgt gatggtggat taccataaac cttctctatc gatctctcag
     8581 ggcctatatg agcaatttga taagattgtg atcattgatc accatcgtcg tggggaagag
     8641 tttccaagta aacctctgct gacttatatc gaatcttcag cttcttcagc ttcagagcta
     8701 gtttctgagc tgatccaata ccaaagcagc aaaacaaatc gcttagatcg ttttgaggcc
     8761 acattattgc ttgcagggat ggttgtagat acaaatagtt tcaatgtccg tacaactgca
     8821 cgaacttttg acgttgccag ctatttaagg tcttgtggag cagacgcatc cttagtacaa
     8881 tatttattga gctcggattt agcgacatac ttggagatca gcaatttgat tgctggaagc
     8941 gactatgtga cttctgatat tgttgtggcg gcagggacag acgaaaaaga gtataatagt
     9001 gttatagcag caaaaacagc agatacattg ctttcgatga tcaatatcaa tgcagctttc
     9061 gtcattacaa agcggacaga cggcttgatt gggatcagtg ccagaagtac cggcacgatc
     9121 aacgtccaaa tcatcatgga atccttaggt ggcggaggac acttcacaaa cgcagctact
     9181 caactggaaa atacgacagt tgaggaaaca aaacagaagc tatttgctgc aatcaaagaa
     9241 aatatgaatc aaatatatga taaggagtaa tccaaatgaa agtaattttt ttacaagatg
     9301 taaaaggaaa aggtaaaaaa ggggacatca aagaagttcc agcaggttat gcacaaaatt
     9361 atctaatcaa aaataacttg gcaaaagaag caacgaaaag cagtatcagt cagttagcag
     9421 gacaaaagaa agcacaagaa aaacacgaag ctgaaatctt ggaagaagct aaaaagttaa
     9481 aagaattctt ggaaaaagaa gatactgtag tcgaattgaa ggcgaaagca ggggaagaca
     9541 gccgtttgtt tggttcgatc ccttcaaaac aaattgctga agggttaaac aaacaataca
     9601 aaatcaaatt agataaacga aaaatcgaat tagaaaatcc gatccgttca ctaggctaca
     9661 caaacgtacc tgttaagctt catcatgagg tgacggctac gatcaaagtt catgtcgtac
     9721 aagaataaca aatcttgata gcgatgttca gctggtaagt ataaccagaa ggaaaaaaga
     9781 caaaccgaaa tttatctgag aaaacagctg cagcaatgaa agcaaaagct gcaaatcgat
     9841 ttcagattga tttcggtttc ttttttggga acagcaaaat agcataaaga aaacgagtat
     9901 aataaaagag aactattttt ttattacact ttcggtacga atgatgactt ttctctcatt
     9961 ttcttgtaaa atgaagcaat tgaagacaag gagcgatacg tatgaatgaa gtgtggcagg
    10021 atcgaatccc accgcaaaat attgaagctg aacaagcggt tttaggctct gtttttcttg
    10081 acgcagaaac gatcattgat gccatggagt acatagagcc aaaagacttt tatcgtcgtg
    10141 gccatcaaat tatttttgag acaatgatcg agttaaatga tcgtaatgaa gcgattgacg
    10201 tggttactgt caaagaccgg ctggaacaag ccaatttgtt agaagatgcc ggtggtttaa
    10261 gttatctctc agacttggca ttagcagttc cgacagcagc aaatatcgtc tactatgcga
    10321 aaatcgttga agaaaaatca ttgctgcgta cattgatcca gacagcaaca ggaatcgtta
    10381 cgaaaggatt cgagcaagga gaagaagttc aaacaatttt agatgatgcc gaacgcagta
    10441 tcttggaggt ttcggaaaaa agaaatcgaa gcggcttttt atctattgct gaagtactga
    10501 actccacgat tgaaaatatc gatcgactag ctcaaaacaa tgaagaaatc acaggtctac
    10561 caactggcta tcaaggtctg gataaaatga cagcaggttt ccagcccgaa gaactaatca
    10621 ttcttgctgc acgtccagcg gtaggtaaaa cagcgtttgc attgaatatt gctcaaaatg
    10681 taggaacaaa aacagatcgt tctgttgcga tatttagttt ggaaatgggg gcagaatctc
    10741 ttgtaaatcg gatgctttgt gcagaaggtt cgattgaggc cagccattta cgtacaggtc
    10801 agctttcgga agaagaatgg tcgaatctga tcgttgcgat ggggagtctt tctcgagcaa
    10861 atatttttat cgatgataca ccagggatca aaatctctga aatccgtgcg aaatgtcgga
    10921 aactagcgca agaaaaaggc aatctaggct tgatattgat cgattatttg cagttgattg
    10981 aaggtaacgg taaagagaac cgtcaacaag aagtatcaga tatctctcgc caactgaaaa
    11041 aactggcgaa agagctgaaa gtacctgtca ttgctctttc acagttatct cgtggagtag
    11101 aacagcgaca agacaaacga ccagtgctaa gtgatattcg tgaatccgga tcaattgaac
    11161 aagatgcgga catcgtagcc ttcttgtatc gagatgatta ttatgaaaga gaaggcggag
    11221 atgacgatga tggaaacgag ccaaaaaatg acaacgtcat cgaagtcatc atcgagaaga
    11281 accgaagcgg tgcacgagga acagttgaac tactgtttat caaagaatac aataagtttt
    11341 cgtcgctttc gccaagagaa gaattttaaa gaagaggttg tgagccttga gcaatcccga
    11401 gagctctcgt aagaagatgc aaggagtaac cgcaggaacg actagagtac ttaatttaaa
    11461 cagatctcca aagggattgt gacaaaagtc ttgtcacagt ccctttttcc gaataaacgg
    11521 tgttcaatca gctgaccctt ggtattagct cagcaaacgt caaaatatga ggaactattt
    11581 tgacgtttgt ttctctaata ctcaggtcaa aacgctgatc ttacgacctc ttttccttaa
    11641 acggtgttct cctgccaact cctcggtcac aagtcacaat atcatcaaac atgagaagca
    11701 gttttttgaa attgctcctt gtgactcaga gttaaacggc aggctcacaa cctctttctt
    11761 aaacggtgtt caaaagcttg catctgcggt aatacgccca atcaaacgaa aaatatgaga
    11821 agctattttt gtttggctgt tcttattact tgatgcagga cagctttttc acaacctctg
    11881 attcacggtg ttccaaaagt agcttcttcg aaaaataagt cgaaatttca caaaaatttg
    11941 agaagcaatt ttcgtacatt ccttcttatt tcttgaagct gaccacttct gttacaacct
    12001 cttttttaat aaccattcta aaaagataaa gttcgttttt taagcgtttt tataaaataa
    12061 aaataaaaat cattcgattt tatttgaaat atgtatctat tattgataca atggttcagg
    12121 attaaataat atagaacgag gtgttcacat gtcatctgta gtagtagtag gtacgcaatg
    12181 gggcgatgaa gggaaaggaa aaatcacaga ctttctcagt gaaaatgctg aagtgattgc
    12241 acggtaccaa ggtggcgaca atgcagggca cacaattaag tttaacggtg tgacttataa
    12301 actgcatttg attccttctg ggatttttta taaagacaag atcagtgtga tcggaaacgg
    12361 cgttgtagtc aatcctaaat cattggttaa agaattagct tatttacatg aaaaaggtgt
    12421 agaaacaagt aatttgcgta tttctgaccg cgcgcacgta atcttgcctt atcatatcaa
    12481 attggaccaa ttacaagaag atgctaaggg cgaaaataag attggtacaa cgattaaagg
    12541 aatcggacct gcctatatgg ataaagcagc tcgtgtaggt atccgtattg cagacttatt
    12601 agataaagaa atttttgaag aacgtcttcg tttgaatcta gatgacaaaa accgtcaatt
    12661 tgtaaaaatg tttgattcag aaccacttga ttttgacgat atttttgaag aatattatga
    12721 atacggacaa caaatcaaac aatacgtcac agatacttcc gttatcttga atgatgcttt
    12781 ggatgcaggc aaacgcgtgt tgtttgaagg cgcacaaggt gtaatgctgg atatcgacca
    12841 gggaacttat ccttttgtta cttcttctaa cccagtagcc ggtggggtaa caatcggaag
    12901 cggtgtaggg ccttcaaaaa tcaataaagt tgtcggcgtc tgcaaagcct atacgtcacg
    12961 tgtcggtgat ggaccatttc caactgaatt atttgatgaa acaggagaac aaatccgcga
    13021 agtaggacga gaatacggta cgacaactgg tcgtccgcgt cgtgtaggct ggtttgattc
    13081 tgtagttatg cgccattcta aacgagtttc aggtattacc aacctatctt taaactcgat
    13141 cgatgtatta agtggattgg aaacagtaaa aatttgtacg gcttatgaat tagatggcga
    13201 actaatctac cactatccag caagcttgaa agagttgaac cgctgcaaac caatctatga
    13261 agaattgcca ggctggtcag aagacattac aggatgcaaa acacttgcag aactgccaga
    13321 aaacgcacgt aactatgtac gtcgtatctc agagcttgtt ggtgtacgta tctcaacatt
    13381 ttctgtcgga cctgatagaa cacaaacaaa catcttagaa agcgtctggg cgcaaatcta
    13441 aggataaaaa cgacgactga atcaggacgt tcttaccaga gcgagtcgaa gtgtttatct
    13501 atacgataat aataacttga acactgttta ttcagaataa gggactgtga caaaaaccgt
    13561 gtcacagtcc ctttttccga a
//
LOCUS       DAZUMM010000071        13148 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000071 DAZUMM010000000
VERSION     DAZUMM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.073"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..151)
                     /locus_tag="ACG8E6_002072"
     tRNA            complement(79..151)
                     /locus_tag="ACG8E6_002072"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(116..118),aa:Lys,seq:ctt)
     gene            532..1101
                     /locus_tag="ACG8E6_002073"
     CDS             532..1101
                     /locus_tag="ACG8E6_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFS5062466.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1088..1948
                     /locus_tag="ACG8E6_002074"
     CDS             1088..1948
                     /locus_tag="ACG8E6_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5062467.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2138..2842
                     /gene="yycF"
                     /locus_tag="ACG8E6_002075"
     CDS             2138..2842
                     /gene="yycF"
                     /locus_tag="ACG8E6_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFS5062468.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2847..4682
                     /gene="walK"
                     /locus_tag="ACG8E6_002076"
     CDS             2847..4682
                     /gene="walK"
                     /locus_tag="ACG8E6_002076"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFS5062469.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4679..5995
                     /gene="yycH"
                     /locus_tag="ACG8E6_002077"
     CDS             4679..5995
                     /gene="yycH"
                     /locus_tag="ACG8E6_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFS5062470.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5996..6865
                     /locus_tag="ACG8E6_002078"
     CDS             5996..6865
                     /locus_tag="ACG8E6_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFS5062471.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6920..7729
                     /locus_tag="ACG8E6_002079"
     CDS             6920..7729
                     /locus_tag="ACG8E6_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5062472.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7832..8461)
                     /locus_tag="ACG8E6_002080"
     CDS             complement(7832..8461)
                     /locus_tag="ACG8E6_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5062473.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8631..9923
                     /locus_tag="ACG8E6_002081"
     CDS             8631..9923
                     /locus_tag="ACG8E6_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5062474.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9999..10661)
                     /locus_tag="ACG8E6_002082"
     CDS             complement(9999..10661)
                     /locus_tag="ACG8E6_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS5062475.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10886..11170
                     /gene="groES"
                     /locus_tag="ACG8E6_002083"
     CDS             10886..11170
                     /gene="groES"
                     /locus_tag="ACG8E6_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFS5062476.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11221..12846
                     /gene="groL"
                     /locus_tag="ACG8E6_002084"
     CDS             11221..12846
                     /gene="groL"
                     /locus_tag="ACG8E6_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFS5062477.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4371 a         2265 c         2833 g         3679 t
ORIGIN      
        1 aataaaaaaa gaacagcgta aatgttttcg aaatgctttc gcaccaacat ttacctgttc
       61 cgtgggtttg gcacccaatg ggccgtgagg ggctcgaacc cgcgacccgc tgattaagag
      121 tcagctgctc taccaactga gctaacggcc cgtacaaaaa gtacttaata atagtagcac
      181 taagaatttt aaaatgcaag actacattta aagaaaatca gaaaaatctt tacttcctta
      241 taggaaatca ttcatagatt attcacattt gctcgctatt atataagcat accaacaaac
      301 aaagttcttg caagagaaca aaccctaaca cttttttcat tttttactcc tttcccgccg
      361 tctccccggc gggtctcctg ttcttaacga taaaagacaa gaaggcttag ccttcttgtt
      421 ttttgttttc atggaatccc gctaatcaaa tcacctagtt aaagcaacta gattgaatgt
      481 ttatcataag tctgatatca tgtttatgaa atgaaaaaag gagcggttat gatggaaaaa
      541 atgcgtcagc aattaatgga gtgggggaag gaactagaaa cattccgtct gcctcactgg
      601 gaagaactcc ctgacttaga actttatatg gaccaagtca gaacgttagt tgatcgttat
      661 ctttcacctg tgatacaagg agaaaagcat ccattgctta cctcttctat ggtaaacaat
      721 tacgttaaac taggtttgat accagcacca gttaaaaaaa gatataacaa agaacacgta
      781 gcatttttac ttgcaatcac tacgttgaaa caagtattga ctattcctga aatcaaagaa
      841 gggatattgt tccaaggaaa aacagtagga attcgtgaag cctataatct attttgcgat
      901 gaacaagaag cagctgtgtg gatggtcagc caattagcac aaggtaaatc tcatccgcaa
      961 aaattcgacc aggcaacccc tgtagaatat attgctgtaa gagcggcaac tttatcgttt
     1021 gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa ccgaatattt aaaagaggag
     1081 aaaacaaatg aaaaacaata aaatcgcaat tttagtagat tcaggtacgg atgtgccaga
     1141 agaattgata aaaaaatacg agatgtatat gatcccattg aaaattattt ataaagatca
     1201 ggtatataca gataaaattg acattacctc tgaagagatc tatcaacgtt tgccagagga
     1261 aatccctggt acatctttgc cggatggtga gtcgatcaca gaaatctttg agcaaatcaa
     1321 aaaagacggt tatgaaaagg tacttgcggt aacaatttcc agtggattga gcgggacata
     1381 taatgtggtt cgtttaattg cacaacagca agaagaattg gaagcatatg tactagatac
     1441 gaaaaatatt ggtattggtg caggtttttc tgcgatccaa gcagcaaaat ggctggaaga
     1501 aggagtagaa tgggatcaac tgatcagtaa tctgaatgag ttagtcgaaa gaacaaaagt
     1561 tttctttaat gttgcgacat tggaatatct tcaaaaaggt ggacggatcg gtttagttgc
     1621 ttctattgta ggaaccgctt taaagcttaa cccaatcatc tcatgtaatg aagaagggat
     1681 ttattacaca gtaggaaaag ctaggggacg taaaaaaagt ttggataaaa cggttgctta
     1741 cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta gctgttgccc atggggatgc
     1801 gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga gagttcccac aagcaaacca
     1861 aatctacttt ggccagattt ctccggcatt agtagtccac actggtccgg gattattggg
     1921 tgtcggtgta caagttttag aaggataaat caaaaaaagg aaacactgag gtgaaaatca
     1981 gtgttttcct tttttattga agttagaaca aaaaataata aagtattttg cactttcatt
     2041 ttaattttct cttttgtttt ataggcaaag atgaaacaaa agcagaaata cggtataatg
     2101 agaaggtatg acaataatat ataagaggtg gctctttatg aaaaaagttt tagttgtcga
     2161 tgatgagaag ccgatttcgg atatcgtcaa attcaattta gccaaagaag gctatgatgt
     2221 ttatactgca tatgatgggg aagaagcctt ggaaaaagtt gctgaggtag aacctgattt
     2281 gattttactt gatttgatgc tacctaaaat ggacggctta gaagttgctc gtgaggtacg
     2341 caaaacttat gatatgccga tcattatggt aacagccaaa gattctgaaa ttgataaagt
     2401 cctaggctta gagttaggcg ccgatgatta cgtgaccaag ccattttcca accgagaatt
     2461 agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg gctgcgaagg aaccagaaga
     2521 ggcagcacct gcggaattga cgattggtga tttgacgatc catccagaag cttacatggt
     2581 gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa ttcgaactac tcttttactt
     2641 ggcgaagcac cttggacaag tgatgacaag agaacattta ttacaaacgg tttggggtta
     2701 tgattatttt ggagatgtac gaacagtgga tgtaactgta cgtcgtttgc gtgaaaagat
     2761 cgaagataat ccaagccatc caaattattt agtgacacgt cgtggtgtcg gttactattt
     2821 gcgtaaccca gaacaggagt aattggatga aaagaaaagt ccgctttttt cgatcagtca
     2881 attttaagat cgctattacc tttattctga ttttattgat ctcgatcgaa attattggtg
     2941 cctactttat acgtggttta gaacgttcca cgatcaatac atttattaaa gatatgaatc
     3001 aaacggtgga atcattagca acaacaatca gcccggaatt gaatcgaaaa gataatgcag
     3061 atgatgaaga ggtaaatgcg aatatcaaaa gatttattga aaacagtgcg actagcgata
     3121 tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg aggaacaacc gatgtcaatg
     3181 aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat caatgatttc acgactaaac
     3241 gttatgttgc tttagataac gacgataaac gggtgaatat caatgttcaa ccgatcctgt
     3301 ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa aagtaatatc gaacaaaaat
     3361 attcggaaat caataacaca gcagtcattt tctttactgc ctcactaatt gcggcattta
     3421 tttcaatgat cgtatctgtt ttagttgctc gttcgattac acagccgatc ggtgaaatga
     3481 gagaacaagc cattcgaatc gctagaggag actacagtcg gaaagtcaaa gtgcatggac
     3541 aagatgaact tggacagctg gctgatacct tcaatcagtt agctgaacgt attgaagaag
     3601 ctcaagatac aatggaagct gaacgcaatc gtttggatag cgtgctatcc catatgacag
     3661 acggcgtcat tgcgacagat cgcaggggta aagtcattac gatcaatgat atggctgttt
     3721 ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc gattttgaca cttttagata
     3781 tcgaagaaga atatacgttg cggaaattat tagaagatcc aaacgaaatg gtacttgacc
     3841 gttcaaccag tacattggaa agtgatcaga tgatcttgcg agtagacttt tctatgatcc
     3901 gtcgcgaatc aggttttatc agcggattag tagctgtctt gcatgatgtg acagaacaag
     3961 aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt ttcccatgaa cttcgtacac
     4021 cgttgacaag tatgcgtagt tatatcgaag cattgagcga aggtgcgtgg aaagacgagg
     4081 aagtagcacc aaattttttg aaggtaacgc tagatgaaac agaccgaatg attcgaatga
     4141 tcaatgacct cttaaatcta tctcgcatgg atacaggtaa tacccagctt caattagaat
     4201 atgtcaattt caacgagtta gtgaattttg ttcttgaccg gtttgatatg atggtgggaa
     4261 atcaggaaaa aaattataag atccgtcgtg aatttacgca aagagatctt tgggtcgaag
     4321 tagatacaga taaaatcatc caagtagtcg ataatatcat gaataacgcg atcaagtatt
     4381 caccagatgg gggaacgatt acttgtcgct tattagaaac gcataataac gtggttttaa
     4441 gtatcaccga tcaaggattg gggattccta aaaaagatct gaaccgagtg ttcgagcggt
     4501 tctatcgagt agataaagca cgtgcaagag cacaaggtgg gacaggactt ggattagcta
     4561 tttctagaga agtgattaag gcgcatcgag gcgctatttg ggcagagagc aaggaaggaa
     4621 aagggtcgac tttttatatc tcgctgccat atgaacctta tgaggaggaa tggtgggaat
     4681 gagaatctct gaaaaagtcg ttcgtatagg actcattttg atggttgcgt taagcatata
     4741 cttctcttat gcaatctggc tgagcccagc aggaaaaagt tcaatcaacg tagatgagac
     4801 aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact tccgaagtct ttcttccgct
     4861 tcatgttaca tggcaccatt cgggaatgat ccaagaaacc aacagtgaaa atctcgtgta
     4921 ccgattgcaa ttgatcatcg ataaagcaag atttggaaag ctttctgaag tagtttcagg
     4981 agacaaatca cagtttgaac aggtgaaaaa tctgaactct ggttttgagt tggcctataa
     5041 tgcgcctttt ttgctaagag aatacaagga agcatttgat ttagatttag atgtgtcgaa
     5101 cttggcaaaa gaaagcgaaa atatgtattt tactagaata caattcgatc aaggaaataa
     5161 aaaaatccga tttttgaatt attcgaacca tctcgtatat gaagcaaata tttcgttgaa
     5221 ttggacagat atcaataaag aattaacagc aaaagacaca aaatggacag aaatgacaac
     5281 agaaccatcg acgatcgaca cgcaatatgc tacgaaacat tcgatcaaac tgaaaaagta
     5341 tagttatatc ctgtcacagc aaccctatac actttttaga aatgcttttt tccagaaacc
     5401 agatagcgtg aaaaataatg atgaaagcaa tgaattgatt ttttacggtg ggaatgaaac
     5461 aatgtctatc cattcggaaa cgcaagtagt tgattttcgt ggagagctgc cagagaaaaa
     5521 agaaaatgct agtgtatttt ctgaaagttt cccctatatt agcaaattag gcagttcttt
     5581 agggaacctc agatattttg accgcagtga caatacgatc gattatcgga tattcgtgga
     5641 aggcttcccg gtttttggga cagacagcag aggacagatt ggtgtggaag ttggcggtga
     5701 aacgaatggt tctgtgcaag tgaatatcca aaccagtttg aatacgattc aagtgccgat
     5761 accttcagac gaagaagtag aacttgcaag tacgaatgaa atcatccagc agttaaaagc
     5821 taagggagca gatagcaaca agattggttc agtgatcgtt ggctatacat ggcaaaatgt
     5881 caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg tatgtgaaat ataaagataa
     5941 ctggtattca gctaacgacc ttctggaaaa attacctgga ttggaggcga attaattgga
     6001 tttcaaaaaa atcgaatgga tatttttcgt cgctttttta gggttgaatg tgtttttatt
     6061 cagcagttat catgacgcgg tataccaaga aaacaatgtc attcgctcta atcagactga
     6121 atcaatccaa caacgtctgg ctagcgacga tatcacttac acggggaaga tttcgtctga
     6181 gaacaagcaa ggctattatc ttagcgcgga gcagaccaat cttagcactt cattagcgaa
     6241 ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt gtgtttttag acggtgatgt
     6301 tttgacgaaa tctttatcca gttcagatgc tgaaaagaac gtgatcgatc cagataaaat
     6361 tagtagttcc ttggaagatt ttttatctaa caaagacaat attttattcg gtaatgatta
     6421 tgtgtacacg aaaaactttt ctcatttaga agaagaacca ctagaaatcc aagcaagcca
     6481 agagtatgaa ggcatcccat tcaatgatga tacagcaaaa atcgttcttt ctttagaaaa
     6541 aaacgataat gattatagca tcacgaaata catgcagacg catttatctg atatcgagca
     6601 gctgcgcgaa aagacggaac tgtatacgga agaggaagca atcaatacat tgtacatcaa
     6661 taacaaaata ccaagaggat caaaaatttt atggcgtcag ctagcttatt cctgtattct
     6721 gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat gtagcgatcg aaacaagcga
     6781 taaagtggtc caaattgaga gtgtcaacgc tttcagcaat acgatcgtga caaacaatac
     6841 tgtaccaaaa gtggaagatc aataaaaaag cgagtataat gaaaaaagtt tacaaagtaa
     6901 gaagggaagt aggagtcaaa tggctgaaga aaaaccagct tttaaaatca gtatcctagc
     6961 aagtggaagt tcgggtaatt cactttatat cgaaagtgaa aaaaaacggc ttttggtcga
     7021 tgcaggactt agcggtaaaa aaatcacttc cttgatggct caaattgatc ggacaccgga
     7081 ccaattagat ggaatacttg tgacgcatga acatcgagat catatacatg gcgtgggtgt
     7141 cttggctcgt aagtatcatt tggatgtttt tgccaatcag cagacttggg aagcgatgga
     7201 accactgatt ggaaatgtac cagtggaaca aaaacatcta tttgagatgg ggaaagtcag
     7261 aacattcggc gatatcgata ttgagagttt cggcgtctcc catgacgcag cggctccgca
     7321 attttatcgt ttccataaag aaaatcggtc attcgtcatg ttgacggaca ccggatattg
     7381 cagtgattat atgcgaggtg tcattgaaaa tgccgatggt tatttgatgg aaagtaatca
     7441 tgatcttgag atgttacgca tgggacctta tccgtggaat ttaaaacagc gtattttagg
     7501 agatagagga catctttcca acgaagacgg tgcacttgtc atgactgatg tgatcggtaa
     7561 tcggaccaaa cgaatctatc ttggccatct tagcaaggaa aacaatatga aagaacttgc
     7621 tcatttaaca atggaaaatg ttttgaaaga aaaagatttt ggcgttggac atgattttga
     7681 aatctatgat acagatccag atgaagcaac tgagctattt tcgatttaaa cagttcaatc
     7741 aataaaaata taaaaagcag aagataaaat caatcagggt tggttttatc ttctattttt
     7801 atttttttag catattaaaa aaataaccgt attatcgact gactacactg atgtgctgct
     7861 tttcatgttt ctcatccagt atctcatcta ctaatgcaat cgcgtaatct gccatgctga
     7921 tcgtactgtc gccagcagcg tttctttgta aataatcatc acttaacaaa taagtacctg
     7981 tacgtttgcc atgtggatca aataaagaag ctggactgat ataagtccaa tgtagatttt
     8041 tagattgttt caattttaac aatccttcgt acatattgaa agctgttgga tagaagggtg
     8101 catttttagg tacttggtca atcatacgag tcgtcttttc tgtatctaag aataacgaag
     8161 aggcaccccc aacgacaatt aagcgtgtat cggttccttc aagcatctca atcaaatggg
     8221 acaaagaagt ttgatgcatt tcttcttttc ctttgggagc gttgaaagca tcaacgacaa
     8281 catcaaaaga tgcaagatca gtagttgtca actcaaataa atccttttgt ataaacggaa
     8341 catctgatgg caatttttct ggatgacgca cgattgccgt cacgtctaaa tctctggaca
     8401 atgcttcgtc taaaataaat gaacctgcat ggccagtagc tccaatgagt gcaattttca
     8461 taaaaaacct ccttttttat tcctgtccat tgtaagcagt tgctgaatat tcgtcaaaga
     8521 atccgatttt tgaaaagctc agtgcgcatt cctttcatgg ttcgtgatca ttgggtatag
     8581 ttaaattaag aaaactgatt gataaaaata gaagttgaag gagataagaa atgaaagagt
     8641 ggttagatcg tgccttttta ccaatgataa catctgttgt gaatcttaga cccttaactg
     8701 ctttgcgtaa tggaatgact tacacgatcc caataattat tgttggctcg ctgtttttct
     8761 tatttgctca ttttcctgtg gattacatcg ctcagtggat agacaaaaca ggattttcta
     8821 tgtattttac acaagtatac caggctacat ttgatgtgat agccttttgg atcgtttttg
     8881 ccattgcata cgcttatgca aaagaagaag acatcgatcg cttaccagtc ggcataacag
     8941 ccttgtctgc ttatctactg cttattcagc cactaaatac tcagatagat actgtctgga
     9001 caggtgctga agggatacta ggagcagtta caactgggct catcatcgga tggggctatg
     9061 cacgtttagt aagacttgcg aaagggtgga agcaaagaga gaagatacct gaaaatgttt
     9121 ggcagtcttt tacttcattg attccaatca ttgttatagt cacttttacg cttatcggtt
     9181 cagcggtttt tcaacagttg ttcagaatga ctgttgttca aggagtatgg acatatgtcc
     9241 agcttcccat tcaatcgttc atagacacgt tgtttggtgt gcttttatta ggtttttttg
     9301 ttccgttttt atggttattt ggtcttcatg gatctgcgat ggtcaatggg ttggttagtc
     9361 ccgtcttgca agcgaattca ttagcgaatg cagaaatctt agcctcagga caagagttga
     9421 cggtggcaaa tggcggacat atcgttactc agcaatttct cgatcagttc atgacggtta
     9481 cgggtgctgg attgacgtta ggggctgtat tttttatgac ggtttttgcg aaatcgaaga
     9541 aatacagaga attaggaaat cttgctcttt taccagcctt tttcaacatc aatgaatcaa
     9601 ttattttttc cacaccgatc gtgatgaacc cgatgatggc tgttcctttt attttcgcac
     9661 cggtcgtttc aggattgatt acctattccg ctttatattt tggattcgtt cctttattta
     9721 cagcagtcca agtcccttgg acaacaccgc cgatcctctc gggtttctta gtaggcggag
     9781 tgcctgcagc gattttacaa ggtatcgtgt tgtcaattag ttttttcatt tatttcccat
     9841 ttttgaaaaa aatagattct gcaaactgca aaaaggaaag acagacaaaa aatgatggag
     9901 cagcagaaaa gatgaacgac taaaaaacaa aaatactcct gtcgctttag attgggaaaa
     9961 tcaccaatct aggatgacag gagtatttat agtcatgttt atactagccc tttttggaca
    10021 gctaattgaa caagaacaac taatgtgttc attcctacgt gtgtgatcat cgatgtccag
    10081 atccgaccag ttgctttgta ctctaggctg aagaagaaac ctaggaaaaa gtagattagc
    10141 agatgaccat cattatgagc aaacgcaaac agcaaagagc tgatcactgc tccgacccaa
    10201 aaatttgaat atcgactgat gatccccagt atactgcggc ggaatacgaa ctcttccatg
    10261 atgggtccag caacagttgt cgccaaaata aaagcgggtg cttccatgat catctggatg
    10321 atattttgtg tattctcgga aggggttgct ttgccaaaca atacagattc taacatgaca
    10381 gcgatgcttt gcagaatcaa tgcgacaaag attccaacca atccataaat aacaatcttt
    10441 ggagcagaag ctttttttac cgctgattcg atcgctaagg gttcacgttg cttaaaataa
    10501 aaaatagcta ggacaacggc gcctagtata tacatgactg cagttgctgt gatcaattca
    10561 gacgtagatt ttacaagtcc tatcgaagct aggatcaagg gtgagataaa aattagacca
    10621 tagcaaaaaa tgctgaggaa gctatatttt tttaaagaca tgtaattcct ccttttaagg
    10681 gataacaagc atgcaaggca agcttatatc gattgatttc aattatatca taatttcaat
    10741 aagcagctgt cagaataaaa tttcttttct ttgatttaaa agcttgcaaa aaagagggag
    10801 aatgagtatt attaaaaatg taggttagca ctcgaaggtt aagagtgcta acagattaaa
    10861 aaaagagtta tcggagggat ttatcgtgtt aaaaccatta ggtgatcgcg tcattatcga
    10921 agtcgcaaaa gaagaagaaa aaactgtcgg aggaatcgtt ttagcttctg ctgctaaaga
    10981 gaaaccccaa actgggacag ttgttgcagt cggtgaaggc cgtttgttag aaaacggtga
    11041 aaaagttcct gccgctgtaa aagcaggcga ccaagttatg ttcgaaaaat acgccggtac
    11101 agaagtaaaa tacgaaggaa aagaatatct gatcgtcgca ggaaaagata tcatggcgat
    11161 tgttgagtaa atttggaaga taaaaaaata atcaaaaata atcaatgagg tgaatcaatc
    11221 atggcaaaag aattgaaatt tgcagaagac gcacgcgcag ctatgttacg cggggtagat
    11281 aaattagcag atacagtgaa agtaacatta ggacctaaag gccgaaatgt cgtactagaa
    11341 aaatcttacg gctctccatt gatcacaaat gatggtgtga cgatcgcaaa agaaatcgaa
    11401 ttagaagatc attttgaaaa catgggtgca aaacttgtat cagaagtagc atcaaaaaca
    11461 aatgatattg caggagacgg tacaacgaca gcaactgttc tgacacaagc aatcgttcgc
    11521 gaaggtttaa aaaacgtaac tgcaggtgct aatcctctag gcattcgtcg cgggatcgaa
    11581 ttggcaacaa aagcagcagt cgaagaattg cataatatct caactgttgt tgattcaaaa
    11641 gaagccattg cacaagtagc agctgtttct tcgggttctg ataaagttgg acatttgatt
    11701 gctgatgcaa tggaaaaagt tggaaacgac ggtgtcatca caatcgaaga atcaaaaggg
    11761 atcgaaacag aactagatgt tgtagaaggt atgcagtttg atcgtggtta cttatcacaa
    11821 tatatggtaa cagataacga caaaatggaa gctgttctag aaaacccata tatcttgatc
    11881 acagacaaaa aaatctctaa catccaagat attctgccat tgttagaaca aatcttgcaa
    11941 caatcacgtc cattgttgat tattgctgac gatgtagatg gtgaagcatt gccaactctt
    12001 gtattgaaca aaatccgtgg aacattcaac gtagtagctg taaaagctcc tggtttcggc
    12061 gatcgtcgta aagcaatgct tgaagatatc gctattttga caggtggtac agtaattact
    12121 gacgatctag gtttagaatt gaaagatgta acaatcgaaa acttaggaaa tgcttctaaa
    12181 gttgttgtag ataaagacaa tacaacgatc gtagaaggtt ctggagaaaa agaagcaatc
    12241 gaagctcgtg tgcaattgat caaaaaccaa attgctgaaa caacttctga ttttgaccgc
    12301 gaaaaattac aagaacgctt agctaaatta gcaggtggtg tagctgttgt taaagttggt
    12361 gcagctactg aaacagaatt gaaagaatta aaattacgca ttgaagatgc attgaatgct
    12421 acacgtgccg cagtagaaga aggaatggtc tcaggtggtg gtacagctct agtgaacgtt
    12481 atcagcaaag tttctgctgt agaagcagaa ggagacgtgg caacaggtat caagatcgtt
    12541 gttcgtgctt tagaagaacc aattcgtcaa atcgctgaaa acgctggtta tgaaggatca
    12601 gtgattgttg ataaattgaa gaacgtggaa ctaggcactg gtttcaatgc tgctactgga
    12661 gaatgggtaa acatggttga agcaggtatc gtagacccaa ctaaagtaac acgttctgcc
    12721 ttgcaaaatg ccgcttccgt ttctgccttg ttattaacga cagaagcagt cgttgcagat
    12781 aaaccagaac cagcagcgcc agcagctcct gcaatggatc catcaatgat gggcggtatg
    12841 atgtaagaaa aagcaacttg cttatctaca actgatcatt agtgaaagac agataaaaag
    12901 aggctggaac agaagcgttt aactccaaga aataagaaga aattcacgga aattgctttt
    12961 caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc gctgtttata
    13021 cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt cccacgctct tttttgtgtg
    13081 taattataat gaaaatagtt atttttaata gttataatat tttatctatt aaaagtaaat
    13141 atccattc
//
LOCUS       DAZUMM010000072        13131 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000072 DAZUMM010000000
VERSION     DAZUMM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.074"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..2199)
                     /locus_tag="ACG8E6_002085"
     CDS             complement(79..2199)
                     /locus_tag="ACG8E6_002085"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5062478.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="ACG8E6_002086"
     CDS             2429..3607
                     /locus_tag="ACG8E6_002086"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFS5062479.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="ACG8E6_002087"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="ACG8E6_002087"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFS5062480.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="ACG8E6_002088"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="ACG8E6_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFS5062481.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="ACG8E6_002089"
     CDS             complement(4960..6213)
                     /locus_tag="ACG8E6_002089"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5062482.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="ACG8E6_002090"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="ACG8E6_002090"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFS5062483.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="ACG8E6_002091"
     CDS             7790..9136
                     /locus_tag="ACG8E6_002091"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFS5062484.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="ACG8E6_002092"
     CDS             complement(9444..9830)
                     /locus_tag="ACG8E6_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5062485.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="ACG8E6_002093"
     CDS             complement(10098..10247)
                     /locus_tag="ACG8E6_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062486.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10709..10921
                     /locus_tag="ACG8E6_002094"
     CDS             10709..10921
                     /locus_tag="ACG8E6_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062487.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="ACG8E6_002095"
     CDS             complement(11199..12998)
                     /locus_tag="ACG8E6_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFS5062488.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4305 a         2360 c         2586 g         3880 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 tttaacaggc tctataattt atgggactga tgaatcagaa ttgattcttc a
//
LOCUS       DAZUMM010000073        13076 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000073 DAZUMM010000000
VERSION     DAZUMM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.075"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      130..296
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            444..1001
                     /locus_tag="ACG8E6_002096"
                     /pseudo
     CDS             444..1001
                     /locus_tag="ACG8E6_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1059..3098
                     /locus_tag="ACG8E6_002097"
     CDS             1059..3098
                     /locus_tag="ACG8E6_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS5062489.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3207..4358)
                     /locus_tag="ACG8E6_002098"
     CDS             complement(3207..4358)
                     /locus_tag="ACG8E6_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS5062490.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4512..5042)
                     /locus_tag="ACG8E6_002099"
     CDS             complement(4512..5042)
                     /locus_tag="ACG8E6_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS5062491.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5141..5491)
                     /locus_tag="ACG8E6_002100"
     CDS             complement(5141..5491)
                     /locus_tag="ACG8E6_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFS5062492.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5648..7177)
                     /gene="ahpF"
                     /locus_tag="ACG8E6_002101"
     CDS             complement(5648..7177)
                     /gene="ahpF"
                     /locus_tag="ACG8E6_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFS5062493.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7187..7750)
                     /gene="ahpC"
                     /locus_tag="ACG8E6_002102"
     CDS             complement(7187..7750)
                     /gene="ahpC"
                     /locus_tag="ACG8E6_002102"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFS5062494.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7894..8556)
                     /locus_tag="ACG8E6_002103"
     CDS             complement(7894..8556)
                     /locus_tag="ACG8E6_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5062495.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8740..10386
                     /locus_tag="ACG8E6_002104"
     CDS             8740..10386
                     /locus_tag="ACG8E6_002104"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS5062496.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10377..11459
                     /gene="uxuA"
                     /locus_tag="ACG8E6_002105"
     CDS             10377..11459
                     /gene="uxuA"
                     /locus_tag="ACG8E6_002105"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS5062497.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11567..13076)
                     /locus_tag="ACG8E6_002106"
                     /pseudo
     CDS             complement(11567..13076)
                     /locus_tag="ACG8E6_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         4374 a         2390 c         2428 g         3884 t
ORIGIN      
        1 gattctttca acttatttgt tatttttttc taaaaaatat taaagattgt taaatttcac
       61 tatttcattt caaattccat tgcctaatca ttaggaaaag tctatcatga actcgtaaat
      121 agaatatagt aataaccttg ttaggtgagg ctcctataca aacataggct gttgcccaga
      181 aacgtcgaaa gtcgccaatg ggtaaaacag aaactgttgg attaaggctt tttccaatag
      241 ctaagaatgt tttctttacg ttgtatagtg ctaaaactcg gacgatgaga tgatgctagg
      301 tcagaattct tgttcgaaaa ggtttctgac cttttcttta tatttttgtg actgacttat
      361 ttgatgagac aaactcgctc ctcacctgac aagtggattg ctgtgtgagg aggagatgcg
      421 gtgaaaaaag atcgattggt ggaggtcgct cggattcact tctggttgca caatacaacc
      481 agaaaacaca acaagcaaaa ctgatctcta tcatgagaga ttcatatgtc gatattccag
      541 gttacggaat gaataagata aatgcagctt actcttatgg gggagttgat ttattaaatc
      601 aaacattaaa ggaaaatttc aaatttgaag ctccctatta tgcaagtatc acatttcaag
      661 attttatcga ttgcgttaat gaactgtttc ctggtggagt caagatcgat gcagaaaaat
      721 ctctagattt agatggcgta tatataaaca aaggagagca agtgatggat ggtaatactt
      781 tactccagta tgctcgattt cgggaagaca aagaaggaga cttcgggagg atcagaaggc
      841 agcagcaagt gatcaaagct atctctcagc agctgaaaga cgtaacttca atattcaagc
      901 tacccaaagc ggttgggaaa ttacttggta gtatccaaac gaaccttcca gaaagtgtgc
      961 ttctggattg tggtatggat ttcctcaaag acatatgtta gaataaacag gtataaatag
     1021 tgaaataaag gaatgacaag caagagaagg aggaaaaaat gaaaagaagt gacaagcacg
     1081 gcaaaaatcg aacaggcgct tatattgccg gcgcagtgat cttaatagca gctgcgggtg
     1141 gcggttattt ttactaccag cactatcaag aaacccaagc agtagaagct ggagaaaaga
     1201 cagttgagca atttgtccaa gctttgaaca agggagatta taacaaagct gcagaaatga
     1261 catcaaaaaa ggcggcaaat aaaagtgcat tatctgaaaa agagatctta gataaatacc
     1321 agaatatata cggtgctgct gatgtcaaag gacttcagat atcgaatcta aaagtagata
     1381 aaaaagacga ttctacttac agtttttcat ataaagcaaa gatgaatacc tcattaggtg
     1441 aattgaaaga tctttcttat aaaggaacat tagacagaaa tgatggtcaa accacgatca
     1501 actggcaacc caacttggtt tttccagaaa tggaaggaaa tgacaaagta agcctgacca
     1561 cacaagaagc agcaagaggg aatattatag atcgaaatgg tgaaccatta gcaacaacag
     1621 gcaaactaaa acaattagga gtcgttccaa gtaaacttgg agatggaggc gaaaaaacag
     1681 ccaatatcaa agcgattgct tcctccttcg acttaacaga agatgctatc aatcaggcga
     1741 tttcacaaag ctgggtacaa cccgattact ttgtcccatt gaaaatcatt gatggagcaa
     1801 cgccagaact tccagctgga gctaccattc aagaagtaga cggcagatat tatcctttgg
     1861 gtgaagcagc tgctcagctg attggttacg tgggagatat cacagcagaa gatattgata
     1921 agaatccaga attaagcagt aatggaaaaa tcggtcgctc aggtttggag atggcttttg
     1981 ataaggatct tcgtgggaca acaggtggga aattaagcat tacagatgca gacggtgtag
     2041 agaaaaaagt tctgatcgaa catgaagttc aaaatggcaa agatatcaaa ttgacaatcg
     2101 atgcgaaggc acaaaaaata gcttttgaca gtctaggagg aaaagctgca tcaactgttg
     2161 cgacaacgcc aaaaaccggt gatcttcttg cgcttgctag ctctccaagc tatgatccaa
     2221 acaaaatgac aaacgggatc tcacaagaag attacaaagc ttatgaagaa aatcctgaac
     2281 atccattcat cagccgattt gcgacaggtt atgctcctgg atctacgttt aaaatgatta
     2341 cagcagcaat cggtctcgac aacggcacta tcgatccaaa tgaagtgttg acgatcaacg
     2401 ggcttaaatg gcaaaaagac agttcttggg gatcttttaa aacaacgcgt gtcagtagtg
     2461 atgtatcaca agtagactta aaaactgctt tgatctattc cgataatata tatgcggcac
     2521 aagaaacgtt gaaaatgggt gagaaaaaat ttcgtacagg cttagataaa ttcatttttg
     2581 gtgaagacct tgatttgcca attagtatga atccagcaca aatttctaat gaagatagct
     2641 ttaactcaga tatcttgcta gctgatactg gatatggaca gggcgaactt ctaattactc
     2701 ctatccagca agcagcaatg tattctgttt ttaccaacaa tggcacactt gtctatccta
     2761 aattgattgc agataaagag acaaaagata agaagaatgt catcagcgaa acagcagtac
     2821 aaacgatcgt gccagatctg agagaagttg tgcaagatgt aaatggtaca gcacattctc
     2881 tttctgcttt agggattccg ttggcagcga aaactggtac agcggaaatc aaagaaaaac
     2941 aggatgtaaa ggggcaagag aacagtttct tgtttgcttt caatcttgat aaccaaggtt
     3001 atatgatggt cagcatgttg gaaaataaag aagatgatga ttcagcaacc aaacgagcat
     3061 cggaactatt gcaatacctc aaccaaaatt atcaataaaa aattgctttt taagtacagt
     3121 aaaaggactg tggcgttatc gtcacagtcc ttttttcaaa taaacggttt tcacgatgtc
     3181 tctttcacaa cttctttttg atttactcac acttcataaa ctttttttgt aaaatatttg
     3241 agaacaagtg gagaaataag tgtacttaat aaaatcacga taactaaagg tgaatagtac
     3301 tgtggttcga tcaattgact ttgctgtcca atttgtaaaa tgatcaaagc catttctcca
     3361 cgcgaaatca taccagcacc gaccatcaat gaactatgtt gagaaaaacc ggcaatctga
     3421 gcaccaaaat aacctccaag caatttggtc aaaatcgcta caagcgtcaa tatagaaata
     3481 aagagtagtt gttcactaaa gtcagaaaaa tcaacttcta atcctacact gacaaaaaat
     3541 actggtataa atactgcata tcctaaagcc tctacattgt ggtatacttc ttctttaacc
     3601 tttgtctgac tgacagctat cccagcaaaa aaagcaccaa tgacagagct aagcccaaca
     3661 agatccgcta cgtaagacat tcctaaacaa ataactagcg atacgatgat aatagaagaa
     3721 tttgcgtaca ctttttctgc aaatgaaagt aggaaaggcg caatccattt gactaataag
     3781 aaaataaaaa caaaataaat tagctgttca atcaaaacaa gcggtaaaga agcattgcta
     3841 gaagcttccc ccgttagaaa agataggctg aaacttagaa ctaaaacgac taaaatgtca
     3901 tctaccacag aagcacccaa aattgttgat ccttcttttg tattcactac gtttagttct
     3961 tttaatactt ctactgaaat actgacagaa gttgccgcaa gaataatccc aaagaaaatc
     4021 gcttcatttc ccgaaacttg gaatgctttg cctgtcaacc agccaaaaac aaccgggaag
     4081 atgatcccaa gcaaagcaac gaacatacca ggtcggaaat attttttcaa aagagataaa
     4141 tcactttcca gaccagctaa aaacattaat aagatcacac caatctccga aaagtcatgg
     4201 actagaatat caggtttgac ccaattcaat ccagcttggc ctagcaaaac accaacaagt
     4261 aattgtccaa tcactgctgg gatacctaat ctccgcgaga tatgtgagcc aatcgttgtt
     4321 gaaatcaata tcaggcataa aataccaata aattccattc tcatcattcc tttgtataaa
     4381 atatagcccg aacttagcat aacatacatt attgaaaaaa taggaaaata tactcacaaa
     4441 aaaaccaaca atccaaaaga tttttttgga ttgttggttt ttatacggtc tattttaata
     4501 actaaaaatg attaacgtat agtcattttt cttattctga tgaaattgat aatctcctgg
     4561 atgcccatga aaatcaaaat aaagccgaat atttggaata gaagcatcaa acttttgaat
     4621 ggattgaaca ataagacgaa tccgccaatc aacaacagaa tactactata aacactccaa
     4681 cttttagctt ttgtattcat gctgaagaac aactggaaca agctgttgat gataacagct
     4741 aatccaagaa gaatcggcag gatagaaacg attgcaggtg caaaagcata gacgatcaga
     4801 gctaagatca ggaaaaaaag accactgaac agaccgatac tccaagaacc tgttttcctt
     4861 ttaaaacggt aagcctcaaa aagattcaac aacccaagca aaacaagata ggcggtgatc
     4921 agataaccga taaaatgaaa aacagcagaa gggtttagca caatcgcaat cccagaaata
     4981 atataaacag cagctcttaa taaggcgtat ttttgaatat tttgtttaat cgtctcaaac
     5041 atataagaaa acctcctttg acttatttta acgtgaacta gtcattttac caaattaaat
     5101 gttacaaaat gagcgaagga gaagaatatc tctggaaaaa tcaacttctg aggatctttt
     5161 ccattttttt gccttttgct agctcatcga tcattttatc taaataacga atttctttca
     5221 tcaagttatc ctcgatgtct tctactcgta caccacaaac aacacctgta atcaaggaac
     5281 gtgaaggatt ggggacaggg gcttccgcaa aaaattgttc gaaagtcact tgtttttgta
     5341 gttgatattc tagttctgtt tgggaataac ccgtcagcca acaaatgatt tcatctactt
     5401 cctcttttat acgatctttt ctctccgctt ttgctacata cataggataa acgcttgcaa
     5461 aacttgtcgt aaaaatacga ttttgcgcca taatcagtca cctttctttt tttcaaatta
     5521 tttactactt atagtatata agaaaaagag aagaaaaagc gattattcaa aaaaacaaaa
     5581 aggattgtga caaaattctt gtcaccatcc ttttttccgc tctctgactt tttacaatct
     5641 tcatagctca agtgcgcatc agataatcga atgctcctaa agctgcagta gctcctgaac
     5701 ccattgaaac aatgatttgt ttgtatggac tatctgtaca atcaccagct gcgaaaacgc
     5761 cggggatatt cgttgcgcca tttttctcgg tcactatttc atttcttgga ttcatttcga
     5821 tcgttctatc tagccaatcc gtattaggga gtaagccgac tagaatgaat actcctgaaa
     5881 cagctagtgt atgcatctcc atcgtacggc gatcgatata ggagagagat tctacctgat
     5941 tcgtaccgct gatttctttc gtcgctgcat gtttgataac agtgacattc gcaagagaga
     6001 ctagtttatc ctgtaagatt tgatctgctt ttaattctgg taagaattct agaacatata
     6061 catgttttgc taacccagcc aaatcgattg ctgcttcaac accagaatta ccaccaccga
     6121 taacagcaat ctcttttcct ttaaataaag ggccgtcaca gtgtgggcaa taagcaattc
     6181 ctttattttt aaactctttt tcgcctggta cattgataga acgccagtgc gcacctgtcg
     6241 cgatgatggc ggtttttgct ttaagtgata taccgttagc taagccaata ttaataaaat
     6301 gttgctcttt atttaaggaa acggcctgtt gattcatcat aatgtcgata gggtaactac
     6361 gcacatgact ttctacttgc tgcatcaatt ttggaccttc tgtgtaagag gtaccaatca
     6421 tattttcaat ccctagtgtt tcaacgactt gtccgccgaa agaatcagcg acaagtcctg
     6481 tacggattcc tttacgtgct gcatagatag ctgcactact agctgctggt ccaccgccaa
     6541 tcactaatac atcataaggt tctttttcaa acaactcttc ttcaatcaaa gggccagtga
     6601 ttttttcaac aatttgttcg atcgtcatgc gtccgctact aaattcttct ccatttaaaa
     6661 acacagttgg cactgccatg atttttttat catcgatttc tttttggaac atcccacctt
     6721 caatcatcgt atgagtaata tttggattta atacagctaa tatattcaat gcctgtacaa
     6781 cgtcggggca attatgacaa gtcaagctaa cataactgtc aaaatgcaat gtttgtgtta
     6841 tttttttgat tttaagactc aaagattcac tgattttcgg tgcccgtcca ctaatttgta
     6901 acatagccag taaaaaagag gaaaattcat gaccgagggg gataccagca aaaacgattc
     6961 cactttcttt gccaactgga ttgatagaaa aactaggtgt gcgcatcgaa tctctttttt
     7021 cccaagagat tttaggagac atttctgcta tttccttcgt aaattcagct agtttttgac
     7081 tattttcact attatccaaa ctaacagaaa aaacaatagg agattcaatc agtgctaaat
     7141 attgttcgag attttgttta gtttctttgt ctaacatatc caccacctaa atctttccaa
     7201 caagatcaaa acttggtttc aatgtttcgc cactttcttt ccattttgcc ggacaaactt
     7261 cacctgggtg ggtacgtaca tattgagcag cgcgaatctt atcaatcagt gcactagcgt
     7321 cacgaccgat tccatctgca ttgatttcca tagcttgaac gattccgtct ggatcaataa
     7381 taaatgttcc tcgctgagct aatccttgct cttcatccaa aacatcaaaa atacgagaaa
     7441 tctgatgaga cggatcaccg atcatcacgt attcgatttt accgatagca tctgatgtat
     7501 catgccatgc tttgtgagta aagtgggtat cggtagagca agaaaagact tcaacaccta
     7561 aagatttcaa tgtactgtat tgttcttgta agtcttctaa ttcagtagga caaacaaaag
     7621 taaaatcagc tggatagaag caaagaatac tccagttccc cataagatct ttttcactga
     7681 cttctaaaaa ttctccttga tgataagcat gtgcattaaa ttcttcgatt tttttcccaa
     7741 tcaatgacat agtaaaacct ccaataataa ttatttattt ataattattg taatttagtt
     7801 ggagttgcaa agtcaatgga tattttgtta tttaataaag attactcaaa aacgaaggtt
     7861 aattataaaa atagaagagg tcgcaagaaa atgctactgc tggaaatact cgggataacg
     7921 ttgagtaaca gtttcctgtt ctttcagcat catgtgtaag tgcaatcccg tttgaaatcg
     7981 agcttcttct ggttttttat caactaaagc cttgagtaaa tcataatgtt gttccatgat
     8041 cgtttcccat ggaagcccct gaatggtcac tcgtaaccag cggaaacgtt ctaaatgggt
     8101 attatggtca tctaaccagt tccaaacttc ttttcttcct gcaatttcga aacaagcttc
     8161 atgaaataaa ttatctgcac ggaaaaaagc gcgttcgtca tttattttga ctgctttttc
     8221 ttgttcttct aaaatcgttt ctagcactat tcgtgtttgt gtgtccaatt tggaacaaca
     8281 ttcaatcatg atttgctctt ccaactgttc tcggacaaag cgagcatttt ctgcagaaac
     8341 aagattaatt ttggaaacat acgtaccact ttgaggtatc gtatacacga gttcttgctg
     8401 acgtagttga atcaatgctt cgcgaatggg agtccggccg atagcaagat gttcctccag
     8461 tgccttttca gatattttcc ttccaggttc taagtcagca tagataattt gtttacgaat
     8521 cgcttgataa gcttgtgatt gcaagctgct tttcatcttt attcctccaa aatcatttct
     8581 atgaacgtag tataacagaa gaggtctgaa aaacaatctg gtatgtcaca ttaactagta
     8641 aatttgtata aatagtaaaa ttttggttga catagcgatt tttattgcat aaaattcact
     8701 tgaatacaaa aactaatatg ttagtttagg aggggaaaaa tgattgattt aaacaactgg
     8761 aggaacttta tccctgaatt atctcaaaaa gaagtagaaa tacctcattt tgaccgtgca
     8821 tcattaagag aaaatggaca aaaaaatcct gtctggatcc atttcggtgg cggaaatctt
     8881 tatcgaggat ttcatgcaga aatcgctcaa cgtttagcgg atctaggcga attgtccact
     8941 ggtgtcattg tttgtgaaac attcgatgaa gaaataattg aaagggctta ccaagatttt
     9001 gatgatttga ttcttgaagt ggtcatgcca agcagtggag agttcgtcaa acgtatatta
     9061 gctgcgacag ctgacagtct gtattgtcat ccagattgtt ctgaacactt tgataagatg
     9121 attcgacact ttgaaaatcc tagtttacaa ttagttactt ttgctattac tgaaaaaggt
     9181 tatcgattga aagatacaaa taataaatat ataggaccga ttgcagtcga tataaaaaat
     9241 ggtccaaaga agccatcaca tacaatgagt attcttgctg tgctacttta taaacgtttt
     9301 ttatctggag cttggccgat tgcacttgtc tctacagata atttttcaca aaacggtagt
     9361 caattacgta cttctttgct aacgatcgca aaagaatggc aaaaggaagg ttatgtacaa
     9421 aatacattta tccaatactt atcgaatgaa gaaattgttg cgtttccatg gacgatgatt
     9481 gatcgtatca ccccgaatcc ttccttgtca gttagcaaaa tgttggcaaa agagggcttt
     9541 tcaaatatgt cgattttcca aaccgcaaaa ggaaataata ttgctccatt tgtcaataca
     9601 gaggacatcc attatttaat catggaagat cattttccca atggacgtcc gaaattcgaa
     9661 aaagctgggg tattcttgac tgatcgtgca acggtagata aagcagatac gatgaaggtg
     9721 acgacttgcc ttaatccttt gcatacgtct ttagctatta tgggttgttt actggggtat
     9781 aggagcatag ctgaggagat gaaagatcca gatttatcgc tgctagtttc tgaaatcggg
     9841 tacaatgaag gcctcccagt tgtggagtct ccaggtatca ttgatccaaa gatctttctg
     9901 gaagaagtaa ttactgtgcg cttttctaat ccaaatattc ctaatatgcc tcagcgaatc
     9961 gccacggata cctctcaaaa ggtggcgatt cgttatggtg agacgattaa aaaatatatg
    10021 gaaatagaag ttaaagaccc gcagacactt gtattcatac cattgactct cgctgcttgg
    10081 cttcgatacc ttattggcat tgatgatcaa ggaaaaccat tcatcccgag tccagaccca
    10141 ctattgacag accttcagca taagctttcc gatattcaac tgggtacgca agatacatca
    10201 catattcatg agaaaatggc tcctattttg tcaaataaag aaatttttgg tgtagatctt
    10261 taccaagcag agcttggcag aaaaatagaa gaaatgttca tcaagatggt agcaggacca
    10321 aatgccgtac gaaaaacatt acaggattac ttacaagaac atggaggaaa tttatcatgg
    10381 aaatgacttt tagatggtac ggatcacagg aagaaaagat taccttagat gaaatcagac
    10441 agattcctgc ggtcaaaggg attgtaggca cattgatgga tataccagta ggagaggtat
    10501 ggcctaggga gaggatcaaa gaactaaaag aagaaattga gtctcgtggg ttaacactaa
    10561 aagtaattga aagcgtcaat gtgcatgatg ccattaaaat tggattacct tcccgtgagc
    10621 agtatatcga aaactataag cagacaatcc gaaacttggc agaatatgga atagaagtca
    10681 tctgctataa ttttatgcct gtatttgact gggtgaaatc tgaccttgac taccgcttag
    10741 aagacggctc atccacgtta gcttttatat ctgatgatat tccagatgat ccaagagaaa
    10801 tcgtcgcgcg catcgaacat tccagcaatg aatttggatt gccgggatgg gaacctgagc
    10861 gcttagctcc tatcaaatcc ttgcttgaag cctatgcatc agtagacgaa gaaaaacttc
    10921 gagaaaattt tgcctatttt ctccaatcaa tcatcccaac atgtgaagag gtaggggtca
    10981 aaatggctgt acatccagat gatccgccat actcgatttt tggattagct cgaatcgtaa
    11041 agaatcgaga agatttagat tggttgtgtc gcatagtaga cagcccttat aacggaatca
    11101 ctctttgtac agggagtatt gcagaagatc cagaaaatga tgtatatggg attttagcag
    11161 aattttgcca aagagatcga atcccatttg cacatgttcg aaatattaaa tttttagcta
    11221 atcgagattt ctatgaggca ccacataaat ccgaatacgg ttcattagac atgtaccgca
    11281 tactaaaaac aatgtatgac aacggattta acggatacat ccgtcctgat catggaagaa
    11341 tgatctggaa tgagacagga agaccaggat acggcttata tgatcgagca ttaggtgctg
    11401 tttatctaaa tggattatgg gaagcaatcg aaaaaggaaa caaaagtaaa gataattagt
    11461 gaaaaaaact gtaattttat tatgtaaata agaaggatgt tcaaattctt gatacataaa
    11521 tccggaggtt gttaataagc attcggattt atgtatctta tttggtctag gaagtaactt
    11581 gattttcttt tttcgattga tccatgagtg cttcttcttt taattcaaag atatattctg
    11641 agatcctcat aatcactttt ttccagtctt ctggtgtgat gattcctttt aaaatataga
    11701 tttttttgat atgagaaggc aattgtttca aagggcagtc gctgataacg atgttgcagt
    11761 tctccataaa atcattttgt attttgatcg attcatttga aaaaatttgt tctatcttaa
    11821 cgatcagatc tttactgata taaaaatcac gcgtatagtt cacaccaatc acaattcgcg
    11881 gctgtttttt gtagcgatca tacaaatgta ctaggtcttc aaccatccat tgcacatttt
    11941 ccttttctaa aataactggt aacaagtgtt gattttgttc tctgaaatcc atataaaatt
    12001 gttcaatggc ggtttggact tctttcaata cgctatgttc ttgataaagc atgaaagtag
    12061 gaaggggcgt atttggttga ttgaagttaa gaaatccatt ataaagttta tttcgtgcga
    12121 atgccaatga gtaaagaatc ttttcttttt caggtataaa caaatcaaat acagtacaaa
    12181 gggactccac catttctctt gaataaagaa catgaggaac gtttgctttt tttaaaagtt
    12241 tgtattgctc caagcttgtt tcttccgatt cgattttcgt gatagaaagt cgagctaaaa
    12301 tattcactaa gatgcgttgt tcgcgacaaa ttggcagatt aggtcgcaaa atatctacag
    12361 agagatcaat aaaaaagtca atatattcat actctgtgaa ttctttttgt acgtctttca
    12421 cagaagttgc aggaaaattc tctttacata tttttaatag aaaatataat ttatctatca
    12481 taccgctatt tagtcggtca agtacttctt ttttaaaaaa agtattcatc ataaaagcca
    12541 ctacttgcgg atcttcatca taaaattctg tagcagtact tgaaaagatg ttccaataaa
    12601 tatctaatgc tgcatattga atttgtacct cttttccaga aaaagacttt ctgccattat
    12661 tagaaaagga gatagacaaa ccgtactatg ctaaaaatgc attaatcttt ttcattttcg
    12721 tatacaaata agaggcacta tagtttgttt gctgactgaa actgataatc gaatttgtct
    12781 gacagctgaa caaagaagtc aagatctgat aactgactga ttcgttgcag tatcttccaa
    12841 atagacgata atagcagtaa gccggatcaa ttccttcaaa attcaacttt aaaaaaggtt
    12901 tttgcttatc aatatgcagt tctgttagat tgagacgctc aaagtcttgt tctaattctt
    12961 tgatgtatag taaaaaattt gattcagaca tatggaaatg atctgctaaa tattgtgtag
    13021 taataaagaa acgatgttca gataggaaaa gaaaaatatc taattttgta ttgctt
//
LOCUS       DAZUMM010000074        12702 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000074 DAZUMM010000000
VERSION     DAZUMM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.076"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2155
                     /gene="acm"
                     /locus_tag="ACG8E6_002107"
     CDS             <1..2155
                     /gene="acm"
                     /locus_tag="ACG8E6_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFS5062498.1"
                     /translation="RNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVDPTNI
                     TDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTV
                     RKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTK
                     PASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPST
                     FEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVS
                     YKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIF
                     KYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEA
                     PNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFK
                     LFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVP
                     SGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEV
                     SEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPG
                     SHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKS
                     ILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2427..2912)
                     /locus_tag="ACG8E6_002108"
     CDS             complement(2427..2912)
                     /locus_tag="ACG8E6_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFS5062499.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3002..3347
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3392..4033
                     /locus_tag="ACG8E6_002109"
     CDS             3392..4033
                     /locus_tag="ACG8E6_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5062500.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4052..4687
                     /locus_tag="ACG8E6_002110"
     CDS             4052..4687
                     /locus_tag="ACG8E6_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5062501.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4705..5520
                     /locus_tag="ACG8E6_002111"
     CDS             4705..5520
                     /locus_tag="ACG8E6_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5062502.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5839..7275
                     /locus_tag="ACG8E6_002112"
     CDS             5839..7275
                     /locus_tag="ACG8E6_002112"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5062503.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7464..9911
                     /locus_tag="ACG8E6_002113"
     CDS             7464..9911
                     /locus_tag="ACG8E6_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS5062504.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10141..11208
                     /gene="rlmN"
                     /locus_tag="ACG8E6_002114"
     CDS             10141..11208
                     /gene="rlmN"
                     /locus_tag="ACG8E6_002114"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFS5062505.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11198..11404
                     /locus_tag="ACG8E6_002115"
     CDS             11198..11404
                     /locus_tag="ACG8E6_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062506.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11401..12504)
                     /locus_tag="ACG8E6_002116"
     CDS             complement(11401..12504)
                     /locus_tag="ACG8E6_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5062507.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         4337 a         2145 c         2703 g         3517 t
ORIGIN      
        1 tagaaatatt attttatcaa tgttatttat tattactaat ataacatcac tgattcctgt
       61 tcatgtctat gcggatgcag gcagagatat cagcagtaat gtcacttcgt tgaccgtgga
      121 cccaacgaat attacagatg gaggaaacat aaaagtaaaa ttctcgtttg acgaaaaaaa
      181 acaaaatatc caaccaggtg attatctttg gatcaattgg ccaagtgaag gaaatattcg
      241 cggtgaagga ttccaaaaag aaatcccatt aatgattgaa aataaaaatg ttggaacttt
      301 aacggtcaga aaagattctg cgcaggttgt gtttaatgaa aatattaaaa acctagactc
      361 agtagaaggt tgggggcaat ttgagataca ggccagaaac gtaaccgata ccggtgaaga
      421 aaatacggga ccattcaccg tgacgagcgg tgataaaaca gctactgtaa atgtgacaaa
      481 gccagcttct ggttcttctt ctagcgtttt ttattataaa acaggagaca tgctacctga
      541 agatactaag cacattcgat ggtttttgaa tattaacaat aacggcactt atgttgaaca
      601 accagttaaa atcagtgatg aaatccaaag tggacaaaga ttagatccaa gtacgtttga
      661 aattaatcaa atacacttag gtgaacagaa ggtttataga ggagaagagg gaatccaaca
      721 atttttacaa gatttcccca gcgctacatt taatttttca gtaactgata attacattga
      781 aataactata ccaaaaaact ttgtcaatct taggaaaata atggtgagtt ataagacgat
      841 catagaaaac ccagaacaaa taaattttga gaatcattcg gaagcttggt ttaaagaatt
      901 taataaacct gctgttgatg gagaaagttt caatcataca gttaaaaata tttcagcttc
      961 tggtggagtc aacggtacag ttagaggcga attgaagatt ttcaaatata tcaatgacac
     1021 agagattggt atcccaaatg tgactttcga attaagacga gcagatgaac agcctattca
     1081 aggacagtct agtatcctgt tgacttcgaa tgaacaggga gaagctagta tcaaaggcct
     1141 gcaagtagga gactatgttg taaaagaaaa agaagcaccc aattggattg attttgatcc
     1201 actaagttca aatgaactca aattctccat aaacgaaaat gatacagagg gagtcagttt
     1261 acctatttat aacaagaaaa aggttacgaa catcacagca actaaaattt ggaacggtgg
     1321 aaccactcca agaccgagta tttattttaa attattcaga gcaagtacaa acaattggga
     1381 accagttcct gatgcagaaa caaaacgatt agacaatgga ataacttcag tgacttggga
     1441 agatatccaa cagtatgatg atagtggaaa cgaatatacg tttaaagtgc aagaagtaga
     1501 tcaaaacgga aatgattatg tcccttcagg ttatcaaaaa attgaaaacg ggctagtcgt
     1561 tacaaatgag aataaagaag tcatttcagt ctcaggacaa aaaacatggg aagacaatga
     1621 gaaccaagac ggcaaacgac cgacgacgat cacagtaaat ctattagccg acggtcaacc
     1681 gatccaacac aaagaagtaa gtgaaaaaga tgattggcgc tatcgtttca caaatctgcc
     1741 aaaatacaga gacgggcaag aaatcatcta tactgtaacg gaagacaacg tgcccgaata
     1801 cagtacgacg attgacgggt ataacatcaa aaacagttat acaccggaaa aaacaaatat
     1861 atctatacca ggtagccata taacaccgtg gaaaaatttt ccagagaaga cagagagaga
     1921 gaaaattaga aatctattct ctcaaaaatt gcaaatattt gctcatacag gagaatccag
     1981 cgaacaaaga agcaattata ttagtaagag agctgtacct aaacagataa caaataaatc
     2041 aaaaagcatt cttcctaaaa caagtagtaa aaaatctagt tttgctgtaa tcattggttt
     2101 attgatagta actatctgtg ctgggatatt cttacaaaaa tataaaaaag tttagttaga
     2161 atataatatt ttttgttaaa tatgactgaa atcaaaagaa taaaattccg atttataatc
     2221 gaaaaaagaa gcatttttat atatgcttct tttttcgtag aatcaataaa tatggatttg
     2281 gctaactatg gaagaatact cgtattagtt ttcagcgttc atactagttg ttacattgtt
     2341 tgattggcca aaagaatttc tgatatattt tgtgctaaat aaagaaaaag acgcttgaat
     2401 tgggaaataa gaaaactact ctatcattac aaattttcat aaactggcag atgttctaag
     2461 taaacaagta tcttttcaaa gtcggaaaaa tccataatca acgtagtggt attgatattt
     2521 ggatgaaagc ctacggtatc ttgacgatca atagcagcat cgatcacgac ttggattttt
     2581 ttctctttgt cgtgcatcaa tgctaatgga gtcactgtac ctgctggaac gcctaggagg
     2641 tcaggaagct gttcttcaga aacaaaagat aagcgctttt cttttaattg ttcagctagt
     2701 tgttttaaat cggcgtgctt ttcatcaggc aagatgacaa gatagatttg ttttcctttt
     2761 cttgatttca ataatagatt ttttacttgc ggaccaggta gggaaaaagg gacatttttg
     2821 actgacgtaa tcgcaggatg atcgactttt tgaaaagaga tacccagttt ttttagtagt
     2881 tcataagctt cttgttcggt tgcacgactc atcgcgcatc accctccttt ttttattagt
     2941 gtatcataaa aaagagttga caaatgaaaa ataatcatgt aaattacacg taaagttaaa
     3001 acaaatgaca tcgaaaagaa gagtagccgt taggtgtttt tagagagtct ctggttggtg
     3061 gaaagaggca gcagttaacc gtgaaacaca tcttggagtc gattttctga agcgtatagt
     3121 aggaaaatcc ggggagcccg ttacagctgc ggtcgttgct agatgaccag ataaggctgg
     3181 cttttccgtg aggagtcggt aaaaaaaggt ggaaccacgt gattaacgtc ctttgatctt
     3241 cggatcaaag gactttttgt tttcatctga acggcaacga ctgtgtgagg ttttttctgc
     3301 aaggaaaaaa taaacagagg tggaaccacg tataatacgt cctcttggca tttgccaaga
     3361 ggattttttt attttaaagg aggaaaagaa gatggagtat ttgattgaag ttatgccgca
     3421 attattagta ggagcactca caacattgaa attattttta tttaccttga ttggctcatt
     3481 accattaggg attgttttag cattaggatt gtctttgcat tttaagccgc tacgctactt
     3541 gcttcaaaca tatgtatggg ttatgcgggg aacaccgctg ttgttgcaac tgatttttat
     3601 tttttatggg ttgccgacga ttgggatcgt ttttgatcgt ttcgaagcag cactcattgc
     3661 ctttatctta aactatgcag cttattttgc agagatcttt cgtggaggga tacaatcgat
     3721 tcctcaaggt caatatgaag cagcgcaagt tttgcaatta agtccctttc aaacggtgac
     3781 gaaaatcgtc atcccacaag tgacaaagat tgttttacct tctgtaggga atgaagttat
     3841 caacttggtc aaagattctt ccttgatcta tatattaggt ttaggtgacg taatgcgtgc
     3901 tgggaaaatc gcgatggaac gcgacgcgac tttactgcct ttgctaggcg tggcaatcat
     3961 ctatcttaca ttgactggta ttctaacaat cgtaatgaaa caagttgaag gaaaattaaa
     4021 ttattaccgc taactgatag gaggaagaaa tatgattgaa ttgaagaaag tgaacaaagc
     4081 atttggtgaa aaaaaaataa tcaaagattt gaatctaatc attccagatg ggaaaacatt
     4141 agctatcgta ggtccttccg gtggcggtaa aaccacgctt ttacgtatct tggctggctt
     4201 agaaagttct gacagcggag aattttattt agacggtgta ccgttcaacc cattcgagct
     4261 taaaatgaaa gaacaagttg taggagtcgt attccaagac ttccagctat ttccacatct
     4321 gaatattttt gaaaacatta ccttagcacc aaaaatggta ttgaaacaga caaaagaaga
     4381 atacaccaaa aaagcagaag aattagcggc ttcattaggg attagtgatt tattgaatat
     4441 ctatccttat cagctttctg gcggacaaaa acagcgggta gcgattgcca gagcattggc
     4501 tatgaatcca cgggtattgg cttatgatga accaacaagt gctttagatc ctgaattacg
     4561 ccagcaagtc gagcgtttga tcctatcatt gaaagaagat ggggtaaccc aaattattgt
     4621 cacccatgat ttagtatttg cagaaaatgt cggtgatcaa ttactaaaag tttccccaat
     4681 tcattaagaa aggagaagat agagatgaaa aaaaccagtt ttctattcgc tttgatcgca
     4741 ggtcttcttc tttttgcagg ctgttcgaac aaaaaaactt cagcagataa ttgggcgact
     4801 atcaacgaag agaaaaaaat cgttataggt ttagacgata catttgttcc tatgggattt
     4861 agagataaag acgggaagct cacaggattt gatattgatt tagccaaagc agtctttgaa
     4921 caatatggga ttacggtaga ctttcagccc attgactggt caatgaagga atttgaactg
     4981 aacaatggaa cgatcgattt gatatggaac ggctattcca aaacgccggc aagagaaaag
     5041 aaagtgcaat ttacaaaacc ttatatggaa aacgatcaag tgctgatcac acctaaagaa
     5101 tcaggaatca caaactttgg acaaatgaat aataagagat taggtgcgca aaatgggtca
     5161 tctggttatg atgtatttac gaaacaacca gaagtattaa aagagatagt caaaaaccaa
     5221 gatgctgttt tgtatgatag ttttaatgaa gcattgatcg atctgaaaag cagacgggtc
     5281 gatggtctgc tgatggataa agtatacgca gactattatt tgaaacaaag aaacgaacta
     5341 gaagacttca acataacaaa aggttcttat gagagtgagg attttgcagt tggtgcacgc
     5401 aagtcagata gtacattagt gaaaaaaatc aatgaagccc tagatcaact agagaaaaca
     5461 ggtgaattcc aaaaaatatc cgataaatgg tttggcgaag atgttacgcc gagaaattaa
     5521 caaatagggg actgtgacaa aagacgtgtc acagtccctt tttccgaata aacagtgttc
     5581 tcctgccaac tccttgtgac tcggagctga atggcaggct cacaacctct tggtaaacgg
     5641 tgttcaaaag ccagtttttc ggtattaagc caaatttatg caaaaatgta gaaagccatt
     5701 ttcacaaaat ttttcttact actcgaaact aagcgacttt ttcacaacct cctttacata
     5761 tttttatttt gttgtggaag gggtttcgtg ttattctaaa agaggaacga ttgaaggtta
     5821 taggaaagga agagatgaat ggaacacaaa gaacttacct catttccaaa agattttctg
     5881 tggggctctg cttctgcagc gtatcaagta gaaggagctt ggcaagaaga tgggaaagga
     5941 gaatcagtct gggatcgatt tgtacggata cctggaaaga catttaaagg aacgaacggt
     6001 gatttggcag tagaccacta tcatcgttat aaagaagata ttgctttaat gaaagaacaa
     6061 ggtctgaaag cttaccgctt ctctgtagct tggacacgga tttttcctaa tggacgagga
     6121 gaagtcaatc aagctgggct cgctttttat gagagattga tcgacgaatt gattgaaaac
     6181 gatattgaac ctatgttgac tttatatcat tgggatcttc cacaagcatt gcaagatgaa
     6241 tataatggtt gggaatcacg tcagatcatt gaagatttca ctcattatgc agaaacatta
     6301 tttgaggcat tccgcgggaa agtccattac tggattagtt tgaatgaaca aaatatcttc
     6361 acttcattag gttatttgtt agcagcacat ccgccaggtg tgacagaccc gaaacgaatg
     6421 tatgaagtta accatattgc taatttagca aatgcatctg tcatcaataa atttcacgag
     6481 ttagaaatgc ctggaaaaat cggaccaagc tttgcgtata cgccaaatta tccaattgac
     6541 agtaatcctg aaaatgttct ggctgctgaa aatgcagaag acctgatggc caattattgg
     6601 atggatgtct atatgtgggg aaaatatccg attgctgcca tgaggttctt agaagaaaaa
     6661 ggatgggcac caacgattga agctggcgat gcagaattgc tagaatctgc taaaccagat
     6721 tttcttggga tcaattacta tcaaacggcg acgaatgcat tcaatccatt aaacggagtt
     6781 ggtgctggaa aaatgaatac gacaggaaaa aaaggttcat cagaagaaac tggagttcct
     6841 ggaatgtata aaaaagttga gaatccattt gtcgaacgaa caaattggga ctgggagatt
     6901 gacccagaag gattaaggat cggactgcgt cgtatcacaa gccgttaccg catgccggta
     6961 ctgattacgg aaaatggctt aggagagtat gacaagttga cagaagataa acaaatccat
     7021 gacgactatc gaatcaatta cttacaaagc cacgtcaaag ccatcaaaga agcaatttcg
     7081 gatggagcag aagtcctagg ctattgcacc tggtcttata cagatttgct tagctggctg
     7141 aacggttatc aaaagcgata tggatttgta tatgttgatc aagacgaaac acaagaagga
     7201 tctttggaac gatataagaa agacagttat tactggtacc aaaaactgat tgaagaaaat
     7261 ggagagaacc tttagttcac atacttaaaa caagaaggta cggctggata tggccgtgcc
     7321 ttttcttgat aaaatcattt agaaattaag aaaaactgta aaataagctg aaaaatgaaa
     7381 gaaagcgtca gttctttcaa acaaaacttt agttccctta cttttttttg tataatgaaa
     7441 cttgatgaaa tgagggataa taaatggcac aaaagaaacg gcctactaaa aaaaggaaaa
     7501 agacgaaaaa aacgaaaaag caaaaacagc agcaggaaca aatgctgacg attggtgttg
     7561 ggtttgcctt gattttattt gctgtcttcg gctttttaaa attaggtttt ttaggaatat
     7621 tgattgcaaa cggctttcgg atcattgcag gaaatactta ccaaatcctc tgtcttttat
     7681 tagccgtatt aggattttgg attgtcatta agaatacaga gttttcaatt ggtaaaaacc
     7741 gacgctggtt tggcggcata ctcttttatt tcggtatctt attattgtta catgcccatt
     7801 tatttggcaa actccatact ggggaaccaa atatcatggg gacgacatgg gatttgttag
     7861 ccagtgatat aaaacaaagt caagtagaca ataacgttgg tggtgggatg attggagcaa
     7921 tcctttatca ttttacttac ttcttgattg cacagcctgg aagttattta gcagcagtgc
     7981 tattactggc aggaggagca ttcctgttca gtaatctgga aggctatcag ctgttaaatg
     8041 gtatccaatc aattggagaa cgagtacagg aattattgga aggtgatcct gccaagcaag
     8101 ctcgaaagca agcggcaaag gaagagagaa tgaaacagcg cgcagaagcg aaagaagcaa
     8161 ggcgcttagc tgcaaaagaa gctgcagaaa aagaagctgc agaatatgag aagaaaaaag
     8221 cggtgagcca gcaaagggaa aatactgctg atgaatggca ggaaccagaa aatacagagc
     8281 cggagcagct aagttttgtt ccaatcgaca gttttcaaga aaatatccat cctgcgaatc
     8341 tagagaaacc agtaccagat acaccaaaac agaccaatac agcagaaggt ttttcagatg
     8401 agttgccaga ggatgacggt acctcattag aatttgagat tgaagcagaa caggaaaatc
     8461 aagactatga attaccgaca gttgatttgc ttgattctat accaactgtg gatcaaagtg
     8521 atgagtataa aaaaattgaa aaaaatatcg gcgtattaga acaaactttc caaagtttcg
     8581 gtgtcgatgc caaggtcgtc aaagccagct taggaccatc cgtcacaaaa ttcgaagtcc
     8641 agccggctgt aggggtaaaa gtaagtaaaa ttgtgaactt aacagatgat atcgcgcttg
     8701 ctttagcggc gaaagatgtc cggatggaag caccgattcc aggtaaatca ctgattggta
     8761 ttgaagtacc aaatggaaag atcagtatgg tttcctttag agaaatcatc gaagcacaac
     8821 caaaccaccc agataaatta ctagaagtac ctttaggacg tgatgtttct ggacgggtac
     8881 aaacggctga tctttcgaag atgcctcact tactcgtggc tggttcgact ggtagtggga
     8941 agtcagtagc aattaatggg attatcacaa gtatattaat gcgtgctaag ccccatgaag
     9001 taaaactgat gatgatcgat ccgaaaatgg ttgaattgaa tatgtacaac ggcatacctc
     9061 atctgttgac gccggtcgtg acgaacccta gaaaagcagc gcaagcctta caaaaagtag
     9121 tacaagaaat ggaagaacga tatgaaaaat tcgcggcaac cggagtaaga aatatttctg
     9181 gttacaatga atttgttcaa cagaaaaact tggaaaacgg aacgaaacat ccaaccttac
     9241 cattcatcgt agtgatcgta gatgaattag ctgatttgat gatggtagcc agcaatgaag
     9301 ttgaagatgc gattattcgt ttagcgcaaa tggctcgggc agcggggatt catatgatcc
     9361 tagctactca acgtccaagt gtggatgtta tcacaggaat catcaaagca aatgtacctt
     9421 ccagaatggc atttgcggta tcaagcggta cagattctcg gacgatcatt gattctaatg
     9481 gggcagaaaa gctcttaggg cgaggagata tgctattctt gccaatgggt gagaataaac
     9541 cgatccgtgt gcaaggagca ttcatttctg accacgaagt agaacgggtc gttcaatttg
     9601 ttacagatca gcaagaagct cattatgaag agaaaatgat gccgacagat gaagttgaaa
     9661 cgacaggtgc tccagaacag ccacaagatg agctgtttga agaagcaaaa gcacttgtcg
     9721 ttgaaatgca aacggcaagt atttcccttc tccaaagaag atttcggatc ggttacaacc
     9781 gagcagcacg tttagtagat gaactggaag ctcatggagt tgttggcccg tcagaaggaa
     9841 gcaagcctcg gaaagtattc attgagcagg aagaaacatc agaaacgttg ccggacgata
     9901 atgacatgta aaacttgtgt gatgaagggt tgaaaaacga ataaccaata aaaagacaag
     9961 gcatgatgat accaaaagaa aaaatggttt ttttccatct tttctaatac ggtatgatgt
    10021 cttgtctttt tagtttttgt gagattaaag ttctattcga tttatttgtg aagtattgca
    10081 ttaaggaaat gaatcatgta tagtatgtag cgaaactttg agaaaaagag ggaaaatact
    10141 ttggaaaaac agtccattta tggcttgacg aatgaagaat taatcaattg gtttatagaa
    10201 aacggcgaga aaaaattccg agctgcccaa gtttgggaat ggttgtatca aaaacgtgta
    10261 agtaatttca cagaaatgac gaatctatcg aaacaattga tcgaaaaatt atcggagcat
    10321 tttatcatca atcctctaaa acagatggtc gtccaagaag cttcggatgg tacagtaaaa
    10381 tatttgtttg aactccctga taaaaacatg attgagactg tactgatgag acaagaatat
    10441 ggcttgtcag tttgtgtgac gacacaagta ggatgtaata tcggctgtac gttttgtgca
    10501 agcgggctgc tcaaaaagaa ccgtgatctg actgctggag aaattgttgc acagatcatg
    10561 atggtccaac attattttga tgagcgcaat cttggcgaac gcgtttcaca tgtcgttgta
    10621 atggggatcg gtgagccatt tgataattac gacaatgtaa tggacttttt gcatatcatc
    10681 aatgatgcca aaggattggc aattggtgct cggcacatta cagtttccac tagtggatta
    10741 gcacacaaaa tcaaggaatt cgctaataac ggcttgcaag tcaatctggc tatttccttg
    10801 cacgcgccga ataatgaggt gcgaacatcg atcatgcgga tcaaccgaag tttcccaatt
    10861 gagaaattga tggaagctgt ggatgagtat ttagagaaaa caaaccgtcg aatcacgttt
    10921 gaatatatca tgttgaatca agtaaatgat agaccagagc atgcgcaaca attagcagat
    10981 ctattaaaag ataagaaaaa attagcatat gtcaacttga ttccatataa tccagtcagt
    11041 gaacatgatc aatattctcg cagtccaaaa gctgatgtat tgagatttta tgatgtattg
    11101 aagaaaaacg gcgtaaactg tgtgatacgt aaagaacatg gtacagatat tgatgcagct
    11161 tgcggacaat tgcgaagcaa gcaaatgaaa aaaacagttg ccaaataatc gtatcctact
    11221 cgttttttca attaccataa aaggagcagt tttcttaatt aaggaactgt tcaacttaaa
    11281 taagagaaaa gattctatag cgaaagctat aaagcttttt ttgacaagaa aaaccctcat
    11341 caagcaaaga aatgtcctca atttgatgac agcctgcttg atgggggttt tagcaattat
    11401 ttaactgatt ttcttatatc cttgctggct ttcttgtgta gcatgataag tttctacgtc
    11461 aagaatggcc tgtttaaatt tttctcgggt gatttcataa ggcacatgtt taatatcatt
    11521 tgttgccatg gctttatcta agattgcttc taaatcggtc gtgacattga gattcatgtc
    11581 ggttaacgat actggaagtt ggttcttttt cataaacaca tagattttat caaacaaatc
    11641 gaactgctga tccatcatca ataaggtcag tacaccataa ctgacagatg ctccgtgaag
    11701 gtatccctcc attttttcta gaacagtcgt accgtaatag aatgagtgtg ccatagcaga
    11761 attatagtta ttgttaacta aaacagaaac caatccggta gtaacaatga tatccgaaac
    11821 gacttgttca attgcatatg agggacgatc gtcggcacta tctttcaatg cttgcgtacc
    11881 ataagtcaag agacgatcaa tgcacatttt tccgatttgg attcctaatc tatccgtgta
    11941 gcctagatca tcgccacgag cagaaaaagt agattcgact tctttggata aagcatcgcc
    12001 aatacctgcc cataagtatt tatcaggtgc ttcggcaata attgctgtat caatgaaggt
    12061 gtgtacaggt ggttttgaag gaaagaagag tccgtccatt acgccttctt cgttatagat
    12121 gacacatacc gcagtgacgg gcgaacagtt cgaaccaata gtgggaaagg tgaagactgg
    12181 tttagctagt ttttcaccaa tgacttttac agtatcgcaa gcacggccac caccgactgc
    12241 aaacaggacg tctgcttttt ggacactttc atgctggatc aactgttcaa tatttccata
    12301 agttgaatta cccccgtacc agagactggc gacaacctct atttcctgtg tcaaagctac
    12361 ttttattttg ttttctgcct ttgaaagtgc agtcttccct ccaatcagca ctacttttgt
    12421 cccatattct ttgacgattc catttgcttt ttcatacgca tctgttccaa tggtataatt
    12481 tggtaagaaa acactttgac tcatattcat ttccttcttt cttcgttgaa acgggttaaa
    12541 attttaaaga gtgatcgatc tgactgcggc tgtagatgga gtagaggtga acctaagaca
    12601 ggataagtga tgaataaggt ggcatgaaca ccttgaaatc atcaataagt atcctaaaaa
    12661 cgaacaagta caaaaacatt gaaaacgaca ataatgaatt tt
//
LOCUS       DAZUMM010000075        12596 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000075 DAZUMM010000000
VERSION     DAZUMM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.077"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            246..818
                     /locus_tag="ACG8E6_002117"
     CDS             246..818
                     /locus_tag="ACG8E6_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFS5062508.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            963..1667
                     /locus_tag="ACG8E6_002118"
     CDS             963..1667
                     /locus_tag="ACG8E6_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5062509.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            1895..2233
                     /locus_tag="ACG8E6_002119"
     CDS             1895..2233
                     /locus_tag="ACG8E6_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062510.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2218..3072
                     /gene="lgt"
                     /locus_tag="ACG8E6_002120"
     CDS             2218..3072
                     /gene="lgt"
                     /locus_tag="ACG8E6_002120"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS5062511.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3219..3452
                     /locus_tag="ACG8E6_002121"
     CDS             3219..3452
                     /locus_tag="ACG8E6_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFS5062512.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3650..5554
                     /locus_tag="ACG8E6_002122"
     CDS             3650..5554
                     /locus_tag="ACG8E6_002122"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5062513.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISVGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            5864..6226
                     /locus_tag="ACG8E6_002123"
     CDS             5864..6226
                     /locus_tag="ACG8E6_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFS5062514.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVFLKLSENLSMKS"
     gene            6243..6854
                     /locus_tag="ACG8E6_002124"
     CDS             6243..6854
                     /locus_tag="ACG8E6_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFS5062515.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(6956..7864)
                     /locus_tag="ACG8E6_002125"
     CDS             complement(6956..7864)
                     /locus_tag="ACG8E6_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5062516.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(7906..8220)
                     /locus_tag="ACG8E6_002126"
     CDS             complement(7906..8220)
                     /locus_tag="ACG8E6_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS5062517.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            8519..10450
                     /locus_tag="ACG8E6_002127"
     CDS             8519..10450
                     /locus_tag="ACG8E6_002127"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5062518.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            10743..11294
                     /locus_tag="ACG8E6_002128"
     CDS             10743..11294
                     /locus_tag="ACG8E6_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5062519.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            11607..12149
                     /locus_tag="ACG8E6_002129"
     CDS             11607..12149
                     /locus_tag="ACG8E6_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR4 domain-containing protein"
                     /protein_id="HFS5062520.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
BASE COUNT         4076 a         2106 c         2630 g         3784 t
ORIGIN      
        1 attaagtata aaaatgacat tttaggccct actttaatga aatttttgtt tggagggaac
       61 gacttcattc ctttgattat ttcagttgaa tttaatagag aagcctggcg tctattaaga
      121 aataattttc ttgatgagcg tcaatttttt taacggaaat ctatcctata ctaaattcag
      181 taaagaaaaa ggaggaatca aagaaagcta ttttaaacaa accaaaatta taggaggaat
      241 aaatcatgac tgaaaataaa gctactcaag aaagaacacc ccaagaaaga ttgcaagaag
      301 aacaagaaca caaggaacat gttcatcata cgaaaattaa tgcagcggcc attgcggatc
      361 atcttttagg caacatgcat acattacatg tgaaactgca ccaatatcac tggtatgtaa
      421 aaggagccaa tttctttacg ttgcatgaaa aatttgaaga gttgtataac gaaaatgaaa
      481 aatggttcga caaacttgcg gagcgtctaa ttacttctgg gcataaacct gcttcgacaa
      541 cggttgaatt tgaaaaatat tcgatgcttt cggaagatgc agttaataaa tacgctaaag
      601 cagaagacat ggttgaaaat attatcgagg acttcagaag cactcgtgac ctaactattc
      661 gcgcgattcg tttagctcaa gacgagggtg acgatgcttt agaagataca ttgattgctt
      721 ttaaaaatga cttagacaag aacatttgga tgctgcaagc gttcattggt aaagaagcat
      781 tagaagatga tgacgctctt gatgaggatg aagattaagg taaaagtaga agctgattta
      841 gggattggaa ttctttccag tcctttttcc attttcttga tgtgcatcaa tttttttgta
      901 gtaagagact tatataataa aagcagcgaa tataaacaaa ataaataaag ggggagaatg
      961 aattgactaa acatacccat catgaaattg gcgaccacgc agcctgtatc cgcttggttc
     1021 caatattcaa tcacttagag gatagcgcga tgaattacat tgctgaaaaa gcccacacga
     1081 aacagtatca aaaaggagag tttctgtttc gttcacaaga aaaagaggat actttatata
     1141 ttatcaatcg tgggaaaatc cgtatttatc gattggcaga ctctggcaaa gagcagctag
     1201 tgcggatctt gaaccctggt gatttcaccg gagaatggac cttgttcaac ccggatgcag
     1261 tacacgagga atatgccgaa gcaacccgag ataccgttgt ctgtatgatt cagcaaaaag
     1321 atgtccaaga ttttctggaa caatacccag ctatttcttt aaaactattg gcggaaatgt
     1381 ctaaacggtt agaaagttca gaacgtcaaa cgacacaagt agccgtggag agtgtcatta
     1441 cccgtttagt tttgtttttg gcagaaaatg tggaacctga aatgggcaac tctcccacca
     1501 tcaccctgcc gatggcaaaa aaggacattg cttcctattt aggaacgaca cctgagacca
     1561 tcagtcgcaa atttggagaa ttggaggacg aaggattgat tcaacagctg tctgggaaaa
     1621 ggatcaagat tcaggattta gatgatttat tgctttacag cgaataatcg gacacacttt
     1681 tttctacacg ttggtgttct gtcgctcttc attaacaacg ggacagaact cagtgctttt
     1741 gcttccatct gtccgttttt ggctttttta agacagaacg accttcattc attggaaaaa
     1801 taaaagcaca aacatgataa ccctcaagtt tttttgtttt atttttcttt aagatagggt
     1861 tgtcgaacaa aacgtaaaaa taagtagggt gaatgtgcat tgtttttggg tgatagttgc
     1921 catcacgttg tcagcagcgg gggcattctt tgtcgtccct ttcaccaaga acacgattgg
     1981 cggcgaagca aaaacgtcta acgttgttga ggcagatagg ttagctatgg aaaaagaaga
     2041 ggaagttgta aaaggagccg gcgtaatcgg acacggaaaa gtggcgtatt acgaagcgag
     2101 tgaccaggga gataaaagtg tattcactgt ttggtttcca tggagcttag ccggaatctt
     2161 gttcagcatc accatgacta gaatgacgcg aaaaattgaa gaaatggagc aaatagaatg
     2221 ggattcgtat taggcgtttt gaatccccat gtggtggaga ttggttctgt tgtgattcat
     2281 tggtatggag tcattattgc agcgggcata ttagcggctc tccagttgag caccaaggaa
     2341 gcaaagaaaa aaggactgga agaggatacc attatcgata tggccttatg ggcaattccg
     2401 attggactcc tcggcgctcg gctttattat gtgttatttg aacttggtta ttatctgcaa
     2461 aatccaggac aaatccttgc gatttggaat ggcggcattg cgatttatgg gggattgatt
     2521 gcaggagggg gtacgctata ttggtatgca aagaaaaaag gtacttcttt ggcactcgtt
     2581 cttgatattt tagcgcccaa tgtattgtta gcccagtcca ttggtcgttg gggcaatttt
     2641 atgaatcagg aagctcatgg tgggccggtt actcgtcaat tcttggaaaa tctttattta
     2701 cctgaattta tcattgaaca aatgaatatg aatggtacct actaccatcc gactttttta
     2761 tacgagtcat tgtggagttt gttgggcttt gtcctcatcg tcactatacg gaaccgaaaa
     2821 cagcttttgc gtcaaggaga agtcgctttg agttacgttt tgtggtactc agtgggtcgg
     2881 ttcttcattg aaggtatgcg aacggacagt ttatggattg gcgagtggat ccgcgtttcg
     2941 caagccttgt ctctggccct gtttattggc gcgattgtag tatggtttat acgcagacaa
     3001 gattatccac caatttctta ttattctgat ggcaataagg ggcagaaaaa gactattaag
     3061 atggtgaatt gagcaaaaag ataaaaagaa gtgcaaaact tgatatgcgt caattttcgt
     3121 ttgctaaaag tgagctatac tacagttgta aagaggaaaa ggcagtgttc attaatctgc
     3181 tgccgataaa ataaaaacac ataaaggaga taggaattat ggaaaaagct atattgcaat
     3241 tagaaacgtt gtcttgtcca agttgtatgc aaaaaattga aggtgccgtg aaatcagtta
     3301 acggtattga taaagacagc attaaagtat tattcaactc cagcaaagtc aaaaccaatt
     3361 ttgattcagc tgtcacttcg attgaagaga ttcaaaaagc tatcgaaaac gtaggctatc
     3421 cggttcttaa agcaaaagtc aaggccgttt aacagattaa atgaactagg caattctgga
     3481 agaaaagcta tttttccagg attgcttttt ttgttgcaat ccgattcgag gtgcttagaa
     3541 acgataaaag agaactttct aaacttgatg tcagtcaatt aattacttag gcaattatct
     3601 tatactgtgt ttatcaataa aagagaaatt aaatttggag ggaaaaagca tgcaacaata
     3661 tatattaagc aagaaaaatg ttatcaccgt catcagtgga ttgttaatcg tactcggttt
     3721 cttcagtcac tttgttttag aaaacgtagg actttcagag tggtctttga tcatcgcctc
     3781 tgtctttggg attacgccaa ttgccattca agcgtttcaa gcaatgaaag tcaaagtcat
     3841 cagtattgat gtcttagtca gtattgcagc gattggtgcg ctttttattc aaaattatga
     3901 agaatcggct attgtcacgt tcttattctt attcggacac tacttggaac aacgaacatt
     3961 gaaccaaacg cgatctgcta tcaaagaatt gactgaaatg gcacccgaaa gcgccttgaa
     4021 acaaatggat aatggcgaat ttgaagaagt agaagtggat gatatagatg aaggggatat
     4081 cttgctcgtt aaaacgggtg cgaaagttcc agtagatggc atagtcctta cgggtgaagg
     4141 gcatatcaat gaagctagca ttacaggtga agctattccg gtcaacaagc tagctgatgc
     4201 agaagttttt gcaggaacca ttttagaaaa cggaacgatt caaatcagag ctgaccgagt
     4261 tggtgaggac accacatttg gaaaaattat tgaactcgtg gaagaagcac aagattctaa
     4321 atccgaagcg gaacggttca ttgatcgctt ttctaaatac tacacaccag ctgtcttggt
     4381 tctggcaatt gttgtatggg cgttcagtca aaatattgag ttagcaatca ccatcttggt
     4441 tctaggttgc ccaggcgcat tagttatcgg agtgcctgtc tcaaacgttg ccggtattgg
     4501 gaatggtgct cgtaacggtg ttcttttaaa agggagtgaa gtcattcaag acttcagtaa
     4561 tgtggataca attgtatttg ataagacagg tactttaact gtcggaaacc caaccgttgc
     4621 agcgactgaa ctgtatgaaa aagatcctgc tgaaacgctt ggctatctgg ccagtgtgga
     4681 acgggaatca gatcatccat tagcaaaagc ggtcttaaat caaattggag aaacaaactt
     4741 ttatcctgtt gaaggaactg aagttgtgaa aggcggcgga atcgtttcaa gtgtagctgg
     4801 acatagagtg gctgttggga atgtggcctt gatggaaaaa gaaaatgtca ctctcagcaa
     4861 aaaagtgcaa aaagatgtca aacggtttga acaacaaggg aactctctcg ttttgacagc
     4921 ggtcgacggt gaattaaaag tactgatggg cattcgcgat caagtccgtc cgggtgtgaa
     4981 agctaatttg caggaattaa aagacttggg cgtgaaaaat ctagtcgttc tttcaggaga
     5041 taaccaagga accgttgatg tggtcgccgg cgaacttggt ttgaccgaag ctcacggcta
     5101 catgttgccg gaagacaaat cggcttatat cggaaaactt caacaacgtg gtcaaattgt
     5161 agcctttgtt ggagatggcg ttaacgatag tccgtcctta gctttagcag acattggaat
     5221 cgcaatggga agcggaacgg acgtagcgat tgaaacatcc gatgtcgttt taatgaactc
     5281 gaacttcagc aacttgcctc acgcattagg attagtaaaa gccaccgcaa ataatatgaa
     5341 acaaaatatc gtgatttcag ttggagtagt gttggtcttg ttgaccagcg tcttcttcag
     5401 cgaatggatg aatatgtcta tcggaatgtt ggttcatgaa ggtagtatct tggtggtaat
     5461 tttcaatggc atgagattaa tgaagtataa gttgaaaggt cgaaaagaac aaaaagaagt
     5521 atcagcacct gcagagaaag taaaaatatc ttaagaataa attagaagag gctgagacaa
     5581 tagtccatcc ttgaaatgac aaaagcggaa actcagattt ttaaatctgg gattttccgc
     5641 ttttttgagt tcttgaaaaa aaataagagt atagagtatg ttctttataa aattaatttt
     5701 cgtgtaaatg attaaaaaat ttaataataa aatttaaatt cctattaagt tgtaacacat
     5761 gaaattgctt ataatagtgt attgaaatta aggtagattg acaaacgagt gatcaaatag
     5821 atatactacc ttcaaacgac cgtttgaatg tgaggtgaga tgaatggaaa aagtttgcca
     5881 agtgactatc gtacataaag aaaaagtaaa tgtaataaaa aaaaaattgg atcaaaaaga
     5941 tttttcaaac ttacttgtgt tagggaaatg tttcagcgat tcttctagga ttaaaatttt
     6001 ctatgcttta gaaacctata aggaaatgtg tgtctgtgat ttagcagaaa tacttactgc
     6061 tagtgtcgct acaacgtcac accatttacg ttttttaaaa aaacatggaa tggcaaaatc
     6121 acgtcaagat ggaaaagtag tttattattc tttagcgaat gaagacatcc tttccgctat
     6181 tcgtgttttt ttaaagttat cagaaaatct atctatgaaa tcttagtttg gaggaagtta
     6241 ttttgttaca aagtattttt tcagctatcg ctgtttatat ttctaccagt attgattatt
     6301 tgtttatctt gctgattatc ttttctcaaa gccacacaaa aaaaggtatt cgtcacattt
     6361 tttggggtca atatcttgga acaggtattt tggtagccgt aagtttgttt gctgcttatg
     6421 tgctaaattt tattccgcaa gattggatca ttggtcttct cggtctaatt ccgattttct
     6481 taggaatcag agtcgctttt gttggcgaag aggaggaaga agaagaggaa gtcgttgaaa
     6541 aactcgaatc tagaggaact aatcgcttat tctggacagt tgccttaata acgattgctt
     6601 ctggaggcga taatttaggt atttatattc catactttac ctcgttagct ttttcagaaa
     6661 tcattattgc tttaatcgta ttcgctatct ctgttgcggt tctctgctat atcagttaca
     6721 aattagccaa gatttctttt gtttccgaaa cgttagaaaa atacgaacga atcattgtcc
     6781 ctataatgtt tattgcgtta ggaattttta ttatgataga gaacgggacg atacaaacgc
     6841 tactaggttt ttaaaaatca taatcacgaa agataaaaag agaggcagcg actaatcaaa
     6901 atcgctgcct tttttgcttt tcaaataatg aagtattttt tagcgtagaa ctatcttacg
     6961 tgttgtggga gtatcccttt tcaagctctt ctttgcttgt agttatttct attgtgatat
     7021 gattctctac gcttgatttg tgtgcgactt ctgcaatttc ttcttttatt tccctagcct
     7081 tgtctatgtc tgaagggtct ataagaaccg tgatgataag ggcgttttct tccccgtcca
     7141 gtgaccaaat gtgaaaatgt gagaggtctt ttacagcagg aacattttta atggagtccg
     7201 ccaagctctt tacgtttaca ttttcaggcg aaccattcaa taaaattttc attgtggtga
     7261 aaaattcagg aacggttttg tagaggatat aaagtgcaat caaaatcgat aagataggat
     7321 ccagacgata tgcctcggta aagttcatta gaatactcac aatcaagact ccaatccacc
     7381 ctaatacgtc ttccagcata tggaggttca acatgctctc gtttttggat gtgcctttgc
     7441 ttaaaatcca cgcagcatat ccattcaatg caatagctac gagagaaagc cagaacatgc
     7501 cactataatt tacgggttgt gggtcaatca aacgtggcac actacgatag atcataaaaa
     7561 acgagccact aattagcaca acgccagtta tcagcgcgcc cagtaaagaa aagcggttat
     7621 agccaaaagt gaaccgttca tcttgttttt tctctgaata accttgaaaa aaccaagcga
     7681 atccaattga aatagagtcc cctaaatcat gaactgcgtc ggacataatg gacacactat
     7741 tgaaaagaaa gccaaagaaa aattcgctta tagagaaaat aagatttatg aagaaagcaa
     7801 ttttaatatt ctttttcggt tgattctgat tcgagtgatt atggtcttta tctgattttg
     7861 acatttaaaa acctcctaag tttgtatgct acatactaat tggttttatt tttctaattc
     7921 attaacatga gttaagactt gttcgagaat actaaaaaca tgaaggtcat ctaaactgta
     7981 aagcatactt tttccttctc ttcttgattt cactaagcgt gcgtctttca acgtttttaa
     8041 ttgatgagaa atagcggact gttccatgtc cagagaaata gcgatatttc caacactaca
     8101 ctcttttttc tgtaaaagaa agagaaccga caatcgtgta ggatcactaa tcaccttaaa
     8161 tagtttgctt attgacagaa tagatccttt atcgattgga gaagttgttt ttatatccat
     8221 agtgttttta ttcctttcat atgaccttcc gttcatatct atattaccat atgagcgaaa
     8281 caatgctaat cttttgttta taaaagagaa gtggcgttct ctttatcttg acaaaatgta
     8341 tatagcgata taaaatgagt atacaaacat atgattaatt agtcatatgt aaggtggatt
     8401 cattacgatt tacttttgga ataaacgaaa ggagtcagaa atagcagaaa tttatgtgat
     8461 tcaagaagta aatgatggaa aacaaaggaa ttataaatta tatagggagg caatagaagt
     8521 ggaaactatc caagaacaac agtctcaaga aaagcataat cataatcaca accacgatca
     8581 cgaccatgat catgggaaaa tgccagtcgt tttgtacttt gttggtttag cattagcagt
     8641 gattgctcta tttttgaatg aagattatca gttggtacaa aatgttttgt tctcaatcgc
     8701 tacaatcagt gccgggtatc acgttattct tcttgaaggc cttggagaaa caattgagaa
     8761 ttcaaaagca caaaaaaagt tcatacctaa ttctcatatt ttaatgggat tagcggcaat
     8821 tggagcttct ttgatgggga atttttggga aggaacatta ttgattctta ttttttctgg
     8881 cgcacatttt ttggaagatt atgcagaagg aagaagcaaa cgagaaataa cgaaattact
     8941 cgaaatgaat ccaacgactg cccggttaat cctgcctgat ggtagtacaa aaaatgtgga
     9001 tgtcagtgaa ttaaaagtgg gagatcaact tcaagtgttg aatggcgatc aagtacctat
     9061 tgatggcgtc attttgtctg gatctacatc cattgatgag tcgtctatta ctggagaaag
     9121 tatcccgaaa gagaaatcca aaggagatgg cgtcttcggg agtacaatca atggaacagg
     9181 taccttcacg atgaaagtca ccaaagaaaa caaagataca gtattttcaa aaattttaca
     9241 attagtgaat cagaaccaag ataaccaaac aaaagctgcg agtattatcc aaaaatttga
     9301 accgaaatat gtcacagttg ttttaattgc cattcctcta ttcatgttat tggctccttt
     9361 tctacttgat tggacatggt cacaaagtgt ctatagagga ttggtccttt tagttgcagc
     9421 ttcaccgtgt gctttagcag cagctactgt ctcggcaacg ctatcgacga cttctaattt
     9481 agccaaaaaa ggagtccttt caaaaggtag ttcttacctg tctcaattag ccgatattaa
     9541 agcaattgca tttgataaga cgggcacctt gaccaaagga aaacctgaag taaccaatta
     9601 ttattttgct gattccgtga acgaagaaaa catgattgat atcgtagtag ctcttgaaaa
     9661 agaatccaat caccccttag cagatgctat tctaagaaag tttgagcaaa aaaacaaact
     9721 aactattgaa gtagaaaacc aaattggtaa aggattgacg ggagattaca aagggagaaa
     9781 ttatcgtata ggaaaaccga cttcgtttga tgatgtttca gatgaatata gtcgtttaaa
     9841 taatgaatgg gcgtcagaag gtaagacagt cgtatacgta gcagtagatg aagaggttat
     9901 aggccttatt gcccttatgg acgttccgag tgaatatgct aaagaaacca ttgaatattt
     9961 taggaaacaa ggtatacaca ccacattaat tactggtgac tcagaaatga cgggaaaagc
    10021 ggttgctaaa caattaggaa tagatgaagt gattgcgaat gtcatgccgg aagacaaatc
    10081 cagaattata gacgaacaaa aagaaaaata cggcgtgacc gccatggttg gagacggtgt
    10141 aaatgatgct cctgctcttg ttaatgcaga tgtgggaatc gctatgggag atgggactga
    10201 tgtggcagta gaggtatctg atttagtttt aatgcaaaac aatttatcta aattggtaca
    10261 atctcataat atttcttcga aaatgaaccg tgttatttgg cagaatatta ttttttcaat
    10321 ggccgttgtt gcttttttaa ttgtcgttag tttcttaggc ttaacggata ttgcaatcag
    10381 tgtcattatt catgaaggaa gtactttggt tgttattttg aatggacttc gattgctaaa
    10441 ggcagtctaa agaatgattg cacaacttca aattaatcga gtaatagaga taattaagga
    10501 ttacactaca ttttttatct ctattacttg tttatattta tctagatgtt caaagtcaaa
    10561 atgatatatc aaccatactc ttgtggcgcg ggttgaatat aagatacatg aaattggata
    10621 aaatcatgta tcaagaatcc tatccttata gggttctttt tttttactcc ccctaaacac
    10681 acgatttcgt gttgtaataa ctataatcat gtattcgaac agactaaaaa ggagggattg
    10741 tcatgggcta taatgttcag ccactacggt cgcaacagga gattaatgat tttttatttt
    10801 gcctgcgacg gaataaaaat gcggaacgtg acgttttctt gtttctaatt ggaattaaca
    10861 gcggtctgcg catgtccgac atcgttaaac tcaagaaaaa agacgtgatt acctcaaaaa
    10921 acccgcgcat cgtggagcaa aagacaggga aaacacgcat tttatactta agcagcttac
    10981 aggacttgat tcacggctat accgcaccct tagatccgga ggcttacttg tttcccagta
    11041 ctaagggcgg ccatttagaa gtcaatacgg tctaccagat gtttcaaaag gtcgcgaagc
    11101 tgttaggaag ggacgatatc ggcacccaca ccctacgaaa aacctttggt taccactatt
    11161 acaagaaaac caaagacgtg gccaccttaa tggaaatctt tggtcacagc agcgagaaga
    11221 ttaccaaacg ctacatcgga attaatgaag atgaaatcag tgaaacgtta ttaaattttc
    11281 ggttaggttt ttgattgtat tatataatag ataagcacag taagacggtt ataatcaccc
    11341 acagtattgg acaaatcaac ctttttttgg tatattatat atacaaatga gagcaggctc
    11401 atagtcaact tgatataaat ccggaacata agggtccggc gggtggcgag caccaaaact
    11461 aaactcgata caaatccgga acgtaagggt ccggcgggtg gcaagcacca aaactaaacc
    11521 tgtacaaaag gctgctcttt tgagcagctt tttttattgg gaaaaaggag cattcttcgc
    11581 acgcggcaaa aaaagaatac aaacgcatgt ctataaaaga gacaacccaa attaccagac
    11641 agttaaacgc gatttataag gctgctcatc tcttacaaga acattttgta gataaaaaag
    11701 tatcctttgt cggagaagtc tcaacggtag cgattatttt ttcgacaact aattttatgc
    11761 acctctgtgg cattgattat cgcagaggca cacacttgtt ttttcaagat gccttagaca
    11821 ggaaaataaa tttacaggat attcaaatca aaacggatgg gacgaccttt cagaaattac
    11881 aagtaatagg tagccttgat ttattattgg ggaagcatat ttctattgtt ggcagaggtg
    11941 tttactcttc tcttcgctat gatgcagcta ttcgaacaag gaaaaaaata ttggctctgt
    12001 ctttgaaaca aaacggatta atctatatcc ctatctcttt gttaaaccta tcatcaaagg
    12061 aaattggacc tggacaaaaa gttactggca tttttagcga ggatttaaca tccggggaac
    12121 ttaaaatgat tatggaagtc atcgattaat cattaatagt gatatgcgac gtcattttga
    12181 agtaattatt ctattttggg ttataaaata aagcatttta agacgatttc aaatcattca
    12241 tgtgggttat ttatcacgga tgagcttgaa atcgtctttt atacgtatca aattgaaatg
    12301 ttcggcatac aacctatcgt tgttactaca ttactaattg tcacttgtta aggaacttgg
    12361 atcctttcta aacattccct agagataaat tttactttat ccctccgtta atatccaatt
    12421 taagcacctt tccgattcag ggttaatttg catatcaagt gcagcttgtg ttctagtaag
    12481 attagccctt ttagtccggc tgagttgact tatggatata agatatggtc atagcaaata
    12541 gatactacgt atagtctgtt aatttagtgt cattaatctt aagttggata ggctac
//
LOCUS       DAZUMM010000076        12237 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000076 DAZUMM010000000
VERSION     DAZUMM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.078"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..556)
                     /locus_tag="ACG8E6_002130"
     CDS             complement(<1..556)
                     /locus_tag="ACG8E6_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFS5062521.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
     gene            complement(572..1051)
                     /locus_tag="ACG8E6_002131"
     CDS             complement(572..1051)
                     /locus_tag="ACG8E6_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFS5062522.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1057..2081)
                     /locus_tag="ACG8E6_002132"
                     /pseudo
     CDS             complement(1057..2081)
                     /locus_tag="ACG8E6_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2098..3204)
                     /locus_tag="ACG8E6_002133"
     CDS             complement(2098..3204)
                     /locus_tag="ACG8E6_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5062523.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="ACG8E6_002134"
                     /gene_synonym="adhC"
     CDS             complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="ACG8E6_002134"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFS5062524.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="ACG8E6_002135"
     CDS             complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="ACG8E6_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFS5062525.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="ACG8E6_002136"
     CDS             complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="ACG8E6_002136"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFS5062526.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8175..8873
                     /locus_tag="ACG8E6_002137"
     CDS             8175..8873
                     /locus_tag="ACG8E6_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS5062527.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9041..11680)
                     /locus_tag="ACG8E6_002138"
     CDS             complement(9041..11680)
                     /locus_tag="ACG8E6_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5062528.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
BASE COUNT         3418 a         2702 c         2193 g         3924 t
ORIGIN      
        1 aagcgcctac gagataacct cccataaaca aagcagagat cgaagcacca ccagcaacaa
       61 gtgaatacat gataaaagca gtacttggag gaatgaccat acctgtcgga gctgacgcga
      121 tattgaccgc tgtcgcaaac ttccggtcat atccttcttc cacttgctga gggatcaaca
      181 ctccaccaat cgcggtagaa gcggcaatgg ccgagctaga gatggaacca aataaagcat
      241 tccctaacac attcgtatga gccaaagccc ccggcacgcg cccgacgaac aacatcgcaa
      301 aatccactag tttcttagct atccctccat tattcatgat aatgcccgag agaacgaaaa
      361 acggaatagc gatcagcgaa aaactattga tacttcccac cattttttga gctgaactaa
      421 aagcagaaac atcaaacggt ataactaaca ataaagtcat catcgatgag aatgcaatac
      481 ttatagcaat cggcatccct aatacaagca acacaacaaa tacaaaaaat aacactaatc
      541 cggcttctat tgccatttta ttcctccatt tttatatttt ttcttgaatt gctggcttgt
      601 tgcttaatgt atcgattaga ttgatcaggc tatagataat gatgagtacc cccgatacag
      661 gtactgacag ataaacatat cccataggaa tacttaaaga aggcgagatc tgccccattg
      721 tcaatgtgac agcattccat ccgccataca ccatgatcat cgcagcaaat accagaaata
      781 aactttgaac aacaacttct aaaattttac tttttttgcc agttaatttt tcacttaaaa
      841 ccgttacggc taaacgtgct ttttgtccca caacatacgc ggaagacaac atggcaaacc
      901 agacaagacc gaatcgaaca agctcttccg tgaccgtatt tggatttcct aaaaccgttc
      961 tggaaaatac ttgccataaa acaacagcga ccatcccaac aagcaaaaca gctcccagga
     1021 tctctaatac cttgtttaaa atcattttca ttacagctac gctccttgct cttggatctt
     1081 ttgataaagg tctttgtagg taggatcgtt tttgaatata tcatgaagtg gggcaactgc
     1141 ctctcgaaac ggtgtattat ctacatcatt gaattggact ccatgttctt cttgtgctaa
     1201 agctttttct tcttcaattg cttttgcaaa agcttcaatt tcaaaaaccg ttgattcttt
     1261 cgctgcttct tcaatggctt tttcctgttc atcagttagc tgttctttga tggcatcatt
     1321 catgatgatg atatctggca cacgtgtatg ctcatcatag gaatagtatt tagctacccc
     1381 gccatgtcca gctgtataca agacaaattc attgttttct gatccgttga tcaaattgga
     1441 ttgaagagac gtgtacactt catcacttcc cataggtacg ggcgaaccgc ctaaaagctc
     1501 taccattttg atagcggttt cgctttgcat cacacggatc ttctttccct tcaaatcttc
     1561 cggcgtatgg atcggaccgt ctaccatata aaagctgcgc tgaccagaat cataataggt
     1621 caatccggta aatcctaaat ttttggtcga ttggtagatt ggctccatta tttcttcatt
     1681 atccatcacc ttaaagaaat gttcttcatc attgaataga taaggaacgg aaaaaatcga
     1741 ataatcttta gagaaacttt ccaacgctga ggcacttact tttgtgaaat ctactgctcc
     1801 tgactgtgtc aattcgatca gttcacgttc tccacccagt tgtccatctg ggaaatactg
     1861 gatctgtacg gaaccatctg ttttttcttt tactaaacgc ccaaattcat tcattgcatc
     1921 aattactggc tgttgcttgc ataagcaaag cgcaaggtaa ctatctcatt tacgtctgca
     1981 tctgcgacag ttgccacact agaattattt ccgcagccgc ttagacctag cagacctagg
     2041 ctaagcgaac ttgctgcaaa cagcagtgat gtttttttca tatgggtttc ctttctttta
     2101 acaataagag acgagcaatt ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat
     2161 tgctgtgatt ccgatattcc gataaaaatc taaactgtcc cccattcctg tcccgattga
     2221 aacgttttga acttctcctt tttcatcgat ttgttcaagc aatccttgga tagcacgata
     2281 agcgacttct ttatattctt gtggtaaata acgtttatgg accgctttca aaataccgta
     2341 agcaaatcct gcagttgcag aagattctac gtaagaagaa ggatcatcca acaacgtatg
     2401 ccataaaccg ctttcagact gataagtttt taatgcacgc acttgtgctt caagtgtttc
     2461 gatcaataat ttgcgcaatg catcttcttt cgataattct aagatttcaa tgatctctgg
     2521 aatagcaatc gtcaaccagc agtttccacg agcccaaaat gcttcggcat agttatgatt
     2581 tccttcaaac gtccagccgt gataccacaa tcctgttcgt ttatccatca agtatttgat
     2641 atggatcata aactggtaac gagcttcttc taaatattct gggcgattca acagtttccc
     2701 gatttttgcc aaaggaagca ctgtcatcat caatgtatcg tcccacaact gattcttatt
     2761 ttccggtcca tatgtggcat gctgtaaccc gtcttcattt gtccgaggca tgtcattcat
     2821 gacccattct gcccattgtt ctaaataagg caaatagcgt gaatctttcg tgtcttcata
     2881 caagtaagcc atagtcaata gcggtgccat tgtattgacg tttttgggcg gagcgccttc
     2941 ttccatacga tcctcaaacc attcattgac cacttgataa gcgcgaggat tttttgtcat
     3001 ctggtaattt ttataaatac catatagacc gacaccttgt ggccaattcc acactgtcca
     3061 gcttttgtca tctactttca gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt
     3121 gatcgttgtc aggttttcaa cgagcaggtc aattttttct ttcactaatt ctttaggtac
     3181 aaaaatctct tctttaataa tcatctttac gccacctttt taaacgcttt cattttatat
     3241 ccttaatata gcagtaatac cgtccactat aaatatcgct ttttgaacaa aaactgtcta
     3301 aaattgacct aaatgttaaa taacaaaagt aactttcagt tacttggaaa ttaaaaaaag
     3361 aacttggaga aacaacgttt tccaagttct ttttcatcta ttttcttatc ttttttctac
     3421 gctttctttt gcttatccgt tgtaagcatc aatgatgatc tgtttcaatt cagagatcaa
     3481 tggttctttc ggattagctg tcgtacattg gtcttcgtaa gctaattcag ccatacggtc
     3541 tacggttgta tctagtgttt cttgtgtgac tccttgagct tttagactca tgttgatgcc
     3601 gacagatttt cctaattcat aaacagccgt tgctaaagct tcgactaatt cctctgttgt
     3661 ttcgcctttc agacctaaga attttgcgat atctgcgtaa tctgtatctg cacggaaata
     3721 gtcatattta gggaacatcg catgtttttg tggatctttt gcattatagc ggataatatg
     3781 cggtaaaaga atcgcatttg tccgtccatg agggatcccg tattccccgc cgattttatg
     3841 tgcgactgag tgacaaatac ctaggaatgc gttagcaaac gccataccag ccattgtaga
     3901 agcgttgtgc attttttcac gtgattccat atcaggtgtt tttactgatt tttctaaata
     3961 gtcaaagact aacttgattg cttgtaggct caatccacgt gtataatctg aagccatgac
     4021 tgaaacatag gattcgattg catgtgttaa cacatccatc ccggtatctg ctgtgacaga
     4081 tgctggaact gacatgacga attgaggatc gacgattgcg acatctggcg tcaatgcata
     4141 atctgctaac ggatatttca catgtgtttc actgtcagtg atgaccgcaa atggcgttac
     4201 ttcagaacca gtccctgatg ttgtcgggat acatacgaat tgtgtttttt ctggtttgtc
     4261 gatcttatat gtccgtttac ggatatccaa gaatttttgt tttgcaccaa agaaagatgt
     4321 gtctgggtgt tcatagaaca tccacattcc cttagctgcg tccattgcag aaccgcctcc
     4381 aagagcgatt actgtatctg gcttgaagtc tgccatcagt ttcgttcctg cgtatacggt
     4441 atttgtcgat gggttcggtt caacatctga gaagacttcg atcttcacat catttttacg
     4501 tcgtgccaat acttcacgaa ctgtatcgca gtaaccgaat tggaccatac cagggtcaca
     4561 aacgatcatg acacgttcaa cgttttccat tttctctaag tacaatagag agttcttttc
     4621 aaagaagatt tttggcggta atttgaacca ttgcatgtta tttctccgtt tcgctacagt
     4681 tttgacattg attaagttta cagcagagac gttacgagaa acagagttct taccataaga
     4741 accgcatccc aaagtcaatg acggaatcat ttcgttatag atatcaccga tgccgccttc
     4801 tgcagatggt gtattcacta aaatacgaca agctttcatt cgaagaccga atttgacatg
     4861 caactcttcg ttttctgaat ggataactgc tgtatgtcct aatcctccaa gattcaacat
     4921 accttcacac aaatcaaacg catggtccgt gttgttagct ttgatcatgg caagaactgg
     4981 agaaagtttt tctcttgata gcggataatc tgggcctacg ccttccaatt cagcaatcag
     5041 cattttcgtt ccttttggta cactgattcc tgccaatttt gcgatttcca tcgcagaatg
     5101 tccaacgatt gatggattga ccgcatattt tccttcgttc atcactgcat cttctaattt
     5161 ttgcaattca tctggtttta cgatataaac ttggtgtgct tggaattctg cttttactgc
     5221 tgcatagatt tctttatcca cgatcacagc ttgttcagaa gcacaaatca ttccattatc
     5281 aaatgttttc gaaacgatca aatcgttcac tgcacgttta atttttgctg ttttttcaat
     5341 ataagctggt acgttacctg gacctacacc taaagctggt tttcctgtag agtaagcaga
     5401 cttgaccatt ccagctccac ctgtcgctaa gacgatcgcg atacctggat ggttcattag
     5461 catagaagtc gcttcgatcg aaggatgttc gatccattgg atacaatttt caggagctcc
     5521 ggcagcgata gcagcatcac gtaccactcg tgctgcttct gcagaagatt tttgggcact
     5581 tggatggaaa gcaaaaacga ttggatttct tgttttcaac gcgatcattg atttgaaaat
     5641 cgttgtggaa gtcggattcg ttgttggagt tactccgcaa accacaccga ctggttcagc
     5701 aatttcaatc aacccttttt ggacatcttc attgatcaca ccaactgttt tatcatgttt
     5761 gatattatgc caaatatatt cggacgcata catatttttg attgctttat cttcgtaaac
     5821 accgcggcct gtttcctcta cagccatctt cgctaatggc atatgttgat ccaaagctgc
     5881 cattgccatt tcatgcacga tatggtctac tttttcttga tcaaagcttt ccatttcttt
     5941 caatgctgca tttgcttttt ctgccaaatc atcaatcatt gcttgtacgt caattgtctt
     6001 cgttttttct ttttctatta ctttagccat cattatcctc ctaatgggtc atggttattt
     6061 gttaaataat tcacatgtat atcatacaat atttattttt ctatgtaaac acttttttgt
     6121 gatttttttc acagttattt ttaaatataa aattgcttta tttacaggtg tttgtataga
     6181 aaaatatata aagttattta ttattattta tatgacacac ccgattattc aatattgagc
     6241 aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta ggacatcttt
     6301 ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc tcttctgtct
     6361 cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt tcttctgttg
     6421 tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc gttttgattg
     6481 ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc cgtttgtcat
     6541 tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt
     6601 tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa cgtgacataa
     6661 aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt cccatttttt
     6721 tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct gataacacta
     6781 gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt tctttattga
     6841 acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt
     6901 gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa
     6961 tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac
     7021 atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt
     7081 tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca
     7141 agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac
     7201 cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata
     7261 cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga
     7321 aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt
     7381 ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg
     7441 cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg
     7501 gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt
     7561 tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt
     7621 cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa
     7681 tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac
     7741 cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca
     7801 actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg
     7861 tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt
     7921 tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa
     7981 aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg
     8041 cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa
     8101 agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa
     8161 aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa
     8221 aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta
     8281 ctattcttct tattatgatt cctattatct tgtttgccac caccatatat gatggatcag
     8341 cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta
     8401 tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt
     8461 tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt
     8521 tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg
     8581 gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta
     8641 tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg
     8701 caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat ctttcattta
     8761 tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat
     8821 acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag
     8881 tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa
     8941 acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt
     9001 atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt
     9061 ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc
     9121 ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg
     9181 gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt
     9241 tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca
     9301 gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt
     9361 ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga
     9421 tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt
     9481 catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag
     9541 caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg
     9601 gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc
     9661 ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag
     9721 cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca
     9781 catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat
     9841 cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga
     9901 tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat
     9961 ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat
    10021 tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca
    10081 tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg
    10141 gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag
    10201 aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc
    10261 gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg
    10321 gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa
    10381 ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt
    10441 cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg
    10501 ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata
    10561 gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta
    10621 cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg
    10681 ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga
    10741 tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa
    10801 cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa
    10861 ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga
    10921 tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag
    10981 tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga
    11041 cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt
    11101 cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt
    11161 ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg
    11221 cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact
    11281 caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca
    11341 aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca
    11401 caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg
    11461 tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc
    11521 gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct
    11581 tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta
    11641 ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc
    11701 ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa
    11761 agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg
    11821 ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag
    11881 aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga
    11941 catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag
    12001 tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt
    12061 gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta
    12121 acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt
    12181 ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg gcttttt
//
LOCUS       DAZUMM010000077        12086 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000077 DAZUMM010000000
VERSION     DAZUMM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.079"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..2080
                     /locus_tag="ACG8E6_002139"
     CDS             62..2080
                     /locus_tag="ACG8E6_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5062529.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    2150..2356
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2507..4921
                     /gene="leuS"
                     /locus_tag="ACG8E6_002140"
     CDS             2507..4921
                     /gene="leuS"
                     /locus_tag="ACG8E6_002140"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFS5062530.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5269..6822
                     /locus_tag="ACG8E6_002141"
     CDS             5269..6822
                     /locus_tag="ACG8E6_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFS5062531.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7203..8987)
                     /locus_tag="ACG8E6_002142"
     CDS             complement(7203..8987)
                     /locus_tag="ACG8E6_002142"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5062532.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(9002..10723)
                     /locus_tag="ACG8E6_002143"
     CDS             complement(9002..10723)
                     /locus_tag="ACG8E6_002143"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5062533.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10823..11584
                     /locus_tag="ACG8E6_002144"
     CDS             10823..11584
                     /locus_tag="ACG8E6_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5062534.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         3966 a         2179 c         2441 g         3500 t
ORIGIN      
        1 ataaatatta agagaagtta taattattaa taatagataa attgaagttg gaggatagaa
       61 gatggaaaac attgcaagaa aagaacggag acgtttagaa cagaccaaac gttttcgaaa
      121 agcaaaacga ggggcagcaa tcgtgggaac tgcgatggta ggctgttctg tcgctgcccc
      181 attagtacag ccggttcctg ttcaagcgga tgaaacacct gttcagttcg gcgcaagaat
      241 caatacagcg gcatttatca cagaaattgc tacttatgct caaccaatcg ctcaagccaa
      301 cgatctatat gcatcagtga tgatcgcaca agcagttgta gaaagcggtt ggggcagtag
      361 tgcgttatct caagcacctt attataatct ttttgggata aaaggcagct atcaaggaca
      421 aaccgtctac atggatacgc ttgaattttt aaataatcaa tgggtaaata aaaaggagcc
      481 tttccgacaa tatccttctt ttgctgaatc tttcaatgac aatgcctatg tacttagaaa
      541 tacttcattt ggcaacggtt attactatgc cggtacttgg aagagcaaca cgaaatccta
      601 tacagatgcg acagcttgtt tgacagggcg ttatgcgact gatcctggtt acgcaggcaa
      661 attaaataat atcatcgcaa cttacggatt gacccaatat gatacaccag ctagcggaaa
      721 cgcaggcggt ggagcgacta caggtggcaa taatactgga agtgcaggta gtactggcgg
      781 ttcaagcaat tctggtaata caaactcagg ttcagcaact tcgaccactt atactgtcca
      841 atctggtgat tctgtttggg gaatctcaaa taaattcggt atcacaatgg atcaattgat
      901 ccaatggaac aacatccaaa acaatttcat ttatccagga caaaaactga caatcaaagg
      961 tagccaggaa aatggctctt cgacgaataa ttccggtaac aataccaact cttcaggaaa
     1021 tgctggtaca tcaaacggag gacaaacaac tggtgcgaaa tacactgttc aatctggtga
     1081 ttctgtttgg aagatctcaa atgatcacgg aattacgatg gctcaattga ttgaatggaa
     1141 caatatcaag aacaatttcg tttatccagg acaacagcta atcgtatcaa atggcggttc
     1201 ttctaatgct ggagcaaata ctggctcaaa tactgggaat acaagtggct ctagttcgtc
     1261 cagctcaagt tcttctaata cagcttcagg aacgaaatat acagttaaag ccggagactc
     1321 tgtttggagc gtctcaaata aatacggaat tacgatggct cagctaatcc aatggaacaa
     1381 tatccaaaat aactttatct acccaggaca acaactgact gtttcaaaca gtggatcaac
     1441 aaacagcacg tcaggtaata caggttcgtc aaccaataca aataacagca gttcagttaa
     1501 tactggcagc agttcgtctc aaggcactta taccgtgaaa gcaggtgaat ctgtttggag
     1561 cgtggcaaat aaaaacggca tcacgatgga tcagttaatc gaatggaata atatcaaaaa
     1621 caacttcatt tatccaggac agcaattgat cgttaaaggt ggaacttcgg caaatacaaa
     1681 tacaggttca actacttctg cagcgaaacc aaatacaccg aatacgtctg ctacgacaag
     1741 tacatcatcc actggtaata cgatgtatac cgtgaaagcg ggcgagtcag tttggagcgt
     1801 agcgaacaaa catcatatca cgatggatca gttaatcgaa tggaataata tcaaaaataa
     1861 tttcatctat ccaggacagc aagtgattgt gaagaaaggt tcatctcaat caacaaacca
     1921 acaatcagtt actggttcga aaacctatac tgtgaaatct ggagaatctg tttggagtgt
     1981 tgctgacagt catggaatta caatggctca actaatcgaa tggaacaata ttaagaacaa
     2041 ctttatttat ccaggtcaaa cgttgattgt agctaaataa aatagctgtt cgaagaaata
     2101 acgattgaat tccacaaaaa tatttgttac tataaggcta cttaaatatg acaacacttg
     2161 aaaaggaagt agtaaaaaga ctgaagtttc agagagcgca tggctggtga gaatgtgcac
     2221 ggatgctttt gaactcgcct ttgagttgcc cttgctgaat acccgagtaa gcaagcggcg
     2281 gtgacggccg ttacaacgtt cgaggccttt tcactaaggt aaaattaggt ggtaccgcgt
     2341 agattacgtc ctataaaaag agcaatcttt ttataggatt tttttatttc ttttgtgaag
     2401 tttgagaaaa caaagcaaaa ggaaataaaa ataaacagat tctgccggtt ttttcgttat
     2461 atttataata gaaacaagtg ttttcaatag aacaggagga cagcaaatga gttacaatca
     2521 taaagaaatt gagaaaaaat ggcaaaaata ctgggcaaaa acgaatactt tcaacaccca
     2581 tgatgatcct gaaaagccaa aattttacgc actggatatg tttccctatc catccggtca
     2641 agggctacat gtagggcatc ctgaaggtta tacggcaacg gatatccttt cacgtgtgaa
     2701 aagaagtcaa ggatacaacg tgctgcatcc aatggggtgg gatgcattcg gtctgcctgc
     2761 tgaacaatat gcgctagata caggaaacga tccagctgag tttacccaaa aaaatatcga
     2821 aacattccga cgccaaatca attctttagg atttagttat gactggaatc gcgaagtgaa
     2881 tacaactgat ccggagtatt ataaatggac acaatggatc tttacgaagc tttatgaaaa
     2941 aggtctagct tatgaagcag aagtagcggt aaactgggta cctgaattag gaacagttat
     3001 ttcaaatgaa gaagtgatcg atgggaaaag tgaacgtggt ggctatgatg tagtcagaaa
     3061 accaatgcgc caatggatgc tgaaaatcac tgcttatgct gatcgcttat tggatgatct
     3121 agaattagtt gattggccag aaagcattaa agaaatgcaa agaaattgga tcggtcgttc
     3181 agtaggagca aatattgaat ttaaagtggc tggtacggat aaatcctata ccgtatttac
     3241 gacacgacca gatactctat tcggtgcaac ttactcggtt cttgcacctg aactagatct
     3301 tgttagagaa attacgaccc ctgaacaaaa agctgctgta gaagcttata tcgaagagac
     3361 agctaaaaaa tcagatctaa agagaacgga tcttgcaaaa gaaaaaacag gagtatttac
     3421 tggtgcttat gcaatcaatc cagttaatgg caaggaaatc ccaatctgga tcgccgatta
     3481 tgtattatct tcatacggca caggagccat catggctgtt ccagcacatg atgaacgtga
     3541 ctttgaattc gctcaaactt tcggactaga aattcttcca gttatcgaag gtggagatgt
     3601 tgaaaaagct gcttacacag aagatggtac gcacatcaac tctgaatttc tagacggcat
     3661 gaacaaacaa gaagcaatcg ataaaatgaa tgaatggcta gaagaaaatg gcgtagggaa
     3721 aaaagaagtc agctatcgct tacgtgactg gctattctcc cgtcaacgtt actggggtga
     3781 accaatccca atcatccatt gggaggacgg aacagtcaca gctgtcccag aagaagagct
     3841 accattacgg ttgcctaaga caaaaaacat caagccaagc ggtactggag aatctccatt
     3901 agcaaatatc gaggagtggg tcaatgtcgt tgatcctgta acaggtaaaa aaggccgtcg
     3961 cgaaacgaat acaatgccac aatgggcagg aagctcatgg tattacctgc gttatatcga
     4021 ccctcataac aaaaaagaac tagcagatta tgaaaagttg aagcgttggc taccagttga
     4081 catctacatt ggtggagcag aacatgcggt acttcattta ctatatgcac gattctggca
     4141 taaattctta tacgatatcg gtgttgttcc tacaaaagaa ccattccaaa aactatacaa
     4201 tcaaggaatg atcttaggcg aaaacaacga aaaaatgtca aaatctcgtg gtaatgtagt
     4261 caatcctgat gatgtggtcg aaacttacgg tgcagataca ctgcgcatgt acgaaatgtt
     4321 catggggcca ttagatgctt cgattgcttg gagcgaaaat ggcttagaag gaagccgtaa
     4381 attcttggat cgtgtatggc gtttgatcgt tgatgagaac aataaaatgc gtgaccgtat
     4441 cactacattg aatgatggca agcttgataa agtgtatcat caaacagtga aaaaagtgac
     4501 agaagactat gaaaatcttc atttcaatac agcaatctcc caattgatgg tctttatcaa
     4561 tgaagcctat aaagtggacg ctctgcctta tgaatatatc gaaggatttg ttcaactact
     4621 cgcaccgatt gctcctcaca tgggtgaaga actatgggca atccttggaa atgatggtgg
     4681 tatttcttac gcaccatggc caacatacga tgaagcagca cttgttgaag acgaagtaga
     4741 agttgttttc caagtaaacg gtaaagttcg tgcgaaaagc aatgttcccc gagacttagg
     4801 aaaagatgag ctagaaaaag cagcattagc caatgaaata gtgcaagaat atattgaagg
     4861 caaaacagtc agaaaagtta ttgcggtacc aaacaagtta gtcaatatcg ttgctaatta
     4921 attggctctt tgtcaataag gactgatgag ttgtgcaaaa tcaaatctgg gccagaatga
     4981 acttcattct gtctcagatt ttttgttatt tcaaattgat taattgattg atcaaaaagg
     5041 ttttgatttt catgttctca aacacgttca atgtcttttt cttctgaatg gtcttgtaag
     5101 agatctctgt aatcatcaaa tttttgtcta ttaatcttag tattttttga tgtataaact
     5161 acctgaaatt gagagtacat accaaattca taaatttttt attagtggga ctagtgattg
     5221 tatggatttt gacgcataat gaatgaagta tgagaaaagg agagacaaat ggatcaagaa
     5281 ctatttatta tcgaagtatt atcagctaaa caaaaaatta tcgagaaagc gaaaaaaaga
     5341 gcgaaaagaa taatgttcaa gcttttaagc ttagtatttt tctctggttt gctttttttc
     5401 ggagctgtat cgttcctata tcgccatatg agtgcagtag tccgttcagc tgaagggatg
     5461 caaaaattaa ataatttgta tcacttttcg tggggagttg gcggaataac aattgttgtg
     5521 cttattgttt ttcttctact tatttggctc cgttaccatc aattgtcgcg tcatttcgat
     5581 caatggctta gtcaaagagc acaacagttt ttacataata aaccaacttc tcaagatgat
     5641 gactatcttt attttgagaa gacttcgacg agtcaagctt ttcggataca aaaaagtgat
     5701 tgccagttat tgctgagatc acttgatgat ttacctatct acttaggcga aagcaaatca
     5761 aaattcggaa tgaacacatt ccatattttt ttactagaaa acgaacccag atgggaaaaa
     5821 agccagtctt cgcctggtgg atacctgaca actaagatga gtacattagt cactggtttt
     5881 ttcctcattc ttttagctgg aacaatctat tatgggagat acactcagcc aaaaatgtct
     5941 tctcatataa atgctgcttc tcacatgaca atgaactcaa ctaacgatac gcatatacaa
     6001 aagacaaatg aagggttgtt agctgatcct aagcaaaagg taaatttgac aacacaaaag
     6061 ataaatgatc tcaaacttga tacagagacg cactctctct atatgacaac agatagtggt
     6121 gctaattggg tatttgtgcc gattgattcg caatggttgc gttttggcga atacaccctg
     6181 acaacggggc tgattcctga aggttattgg atggacaaca catatgatat ttccgccgat
     6241 ttctcatggt ttatctattc ttctggggat gatgtacgtc tgctgacttc aagagataat
     6301 ggtaaaacat ggcaaacgtc tattatcacc caatcagttc agccgatacg ttatcgaaaa
     6361 gcggtatttt atggcaacga tggtctattt atctgtacat cagccagtgg gatgtctgca
     6421 gaaactttgt acatttacac tactatggat ggcggaatca cttggataaa aagtggaagt
     6481 acaatgatca atcagccagt acaaaatgtc agcttcgtta caacttcttt aggttttgtt
     6541 tcgacacgtg aaagtttgtt ttatacaaac aacggaggac gctcattcaa agaatcagtt
     6601 ctgtcgattc cggaaaacta tcagacaggg gggatagatc tttttcaatc accaaatgaa
     6661 gttactcaaa taagcgcgaa tacgttagag acaaaatttt atttgttgaa aaaaggacca
     6721 atcgatgcag ggaaaatgtt tgcctgcctc ttccgttcaa ctgataacgg ggaaacatgg
     6781 caattcgttc agcaactatc gcaagtagaa caaggagatt aaagaagaga gattgtgagc
     6841 ctgacgttca gcaccgagtc acaagaaaca aattcaaaaa acagcttctc atatttgttg
     6901 aatagtgtaa ctcgtgattc ggagttgaca agagcaacaa gaagcgattt tgaagtatag
     6961 cttctcgtat tttacaagat cgtgtcttat tgccgatagg ttgtctcttg aacaccgttt
     7021 attcgtaaaa agggattgtg acaagtcttt tgtcactact taccgaacga aactgaagga
     7081 gctatccaaa aagtatcatt agtattattc tttgtgtttc tagtggcacc actagtacat
     7141 ttaaaaaagc tatcgatcat ggactaatca tacatgatcg atagcttttt agataatgtt
     7201 tgttacgcta tctgaaattg actattataa agactggcat aaagcgttgg tgattggagt
     7261 agttcagaat gtgttccttt ttcgacgata tcgccattct tcattacaag gatcatgtct
     7321 gcattttcaa tcgttgacaa acggtgtgca atcacaaaac ttgtcctatc ttttgtcaca
     7381 gcttccattg cttgctgaat caatgcttct gttcgtgtat ctacgctaga agttgcttca
     7441 tctaaaataa cgacaggagg attcgctaga atgactcgag caattgtcaa taattgttgc
     7501 tgtccttgag ataacgtgcc gttttcgcta gaaatgatcg tatcatatcc gtctggcagt
     7561 gttcgaataa agtgatcaca ttgtgcgatt tcagccgctt cgataatttg ttctcttgtg
     7621 gcatccattt tcccgtaggc gatattatca gctaccgttc cttcaaacag ccaggtattt
     7681 tgtaaaacca tcccaaaaga acgacgtagt tctccacgtg aaagcttggt aatatcaaga
     7741 ccgtcgaatt cgatctttcc tttattcagt tcataaaaac gcatcagcag attgactaaa
     7801 gtcgtttttc ctgcaccagt tggaccaacg atcgctaccg tttgttttgg ctttactgaa
     7861 aaatcgactt gattcatcaa tattttatct gatgtgtaac caaactgtac atttttgaaa
     7921 gctatttttc ctttcggttg ttctaaaagt cttgcatctg gaaggtctgg aatttcatct
     7981 gtttcatcta atatctcaaa tatcctatcg attgctgcca ttgcggattg gatcgagttg
     8041 atcacataag aagcggtaga aattggttca gaaatctgat tgatgtactg aagataagct
     8101 tgtaagaagc cgatcgtcac accacctgat agaacaagca tagcaccgaa taccgcactt
     8161 atgatgaagg cgagttgatt aatgaaacga atggccggat agatagcaca gttcataaat
     8221 tgagcacgtt taaaagcgtt gtattgcttt ttatttactt cgtgtatagc agcttctgca
     8281 tgagcttgct gattgaatgt tttgaccact agattaccag ctaagatttc ttccattcga
     8341 ttatttaaaa cacccagttc atactgattc tgttcagaaa tctgtttgtt tttgttcgca
     8401 atcttagttg taacaattga actgccaacg atcagcaata agacgagcaa agtaagcttg
     8461 ctttcgatat aaaacagcat cagaccagca gcagctattg ttacgacaga agtaaaaaat
     8521 tgattgatac ctgtcaataa cacttgagaa agttgattga gaccggtagt tgtccgacta
     8581 atgatgtcac ctacttgatg attgtcaaaa aacgacatcg gtaatgtttt gaattttttt
     8641 gtgatttctt ttcgcaccct caatgtgatc ttttcgctta acgctgccat tgtccgttcc
     8701 tgaataaatg aagtgatgct gcttaagact gaacagccga tcagcaataa gacagggaac
     8761 aacaatgctt gtttgatatc actgaaagaa agctgtccaa tacctgtttt tcttatttct
     8821 gataagagat catcgatccc gattcccatg atcaagggca tggctacgac taataggttc
     8881 ccgaataaac tgcatagaaa caatccgtaa aaatatttat gttcaggttt gatcattttc
     8941 caaaaacgta tcaaagcacg cgtgttcaac gtttttttct tttccatcgt atcccttctt
     9001 tctatattcc ttgtgatttt gcaaactcct gataagtttg gcaattagac agtagttctt
     9061 catgagtacc ttgtccaacg atttttcctt catctaaaac aatgatgtta tctgcttgtt
     9121 ggattgtact gaggcgttga gcaacaatca cgagagtttt ctccgacatg tgtttatgaa
     9181 gggctgctct taatgctgca tctgttttat agtctaaagc ggagaaacta tcatcgaata
     9241 catagatctc tgctggtttg atcaacgctc gagcaataca tatccgctgt ttctggcccc
     9301 ctgaataatt agaaccgcct tgagcaacaa agctgtccaa tccgtcagga agggtgtcca
     9361 ctagttcttc taattgagcg atcttgatcg ctttagacaa ctcttctgtt gaggcatatt
     9421 catttcccat ctgcaagttg cttaaaattg tcccgctgaa taagaaagcc ttctgaggga
     9481 cataactgat gtgggagcgg atctgttctt gagtcatttg agtaagagga ataccaccaa
     9541 gggttacttt tcctatcgta gggtcactaa gtctcaagag aagtttcgcc aaactacttt
     9601 ttccagatcc ggtcccgcca acgatcgctg ttgtttttcc tttaggtatt gtgaaatcaa
     9661 tatcttctag aactggatca gagatttcag tatatttaaa tgtgacatgt tcaaaagcag
     9721 ctgcatttgt ctgatcaaag gttggttcag ctactgtttt gatcgtctct tcctcaattt
     9781 cgatctctgc ttgcagtatt tcttctatcc gtcgtaatga ggcaaacgat ttagggataa
     9841 tgaccaatac catagctgcg atcatcaagt aagcaagtgt cagcatagag tattcgacta
     9901 cagcagtcac gacaccgatt tgtaatgctc cttctgctac aagatttccg ccaaaccaca
     9961 aaatagaagc ataaacgatt cccatgagca aaaaagcaat tggagtgacc caagcaaata
    10021 ttcggttcac tttgatcatt tgattagcat agtcagcaaa gctcttattc gttcgctttt
    10081 cttcatatga ttgattatca aaggcacgga tcatattaat cccagtaaag aattctctca
    10141 atgtgatggt gatccgatcc atttttggct ggatactcaa agataaaggt gcccctcgtt
    10201 tcatcagtcg ataaacaatc catacataaa aaacaacaga gatcaatgta acccatgtta
    10261 gtaatagaga ataacgaaac atcatgagaa tggtgaaaac gcaaataacc ggtgctggta
    10321 agatcatttg gcaaaataaa acaatcattt gctggacatt atcgacatca ttcgtcattc
    10381 ttgtcaaaag agatccagaa ctaaaacgac tgatatcctt cattgacagc tgttggaatt
    10441 tacggaaata tttctcccgc gtttggaatc caaacctagc ggaagccaat gctgaaaagt
    10501 aactagaggc aactgcagcg attgttccta gaaacgctac aagcagcatt tcaactccaa
    10561 ttttttttat aactgaggct tcgccacttg ctatacctgt gtcgatcagc tttgctacta
    10621 attttggtac acctaatgta ccggcgattt ctaaacagat catcacaaag gtaaggagag
    10681 cgagtccttt gtttttctgt aagaatgttt ttaagataga catattattc ctcctttttt
    10741 cctgatactt ttcgattcta taccttcaag taacttgaag gtcaatagaa aaaagaataa
    10801 actgaaaaag aggtgagaaa aaatgaaaaa aatgtatacg attggccaag tatcggaact
    10861 atttgatttg ccaaaatcaa cgatccgcta ttgggatgaa caaggattaa tccgttcaag
    10921 tagacaagaa gagaatgatt atcgattgtt tgatatcgat gatattttta tgctttatga
    10981 tatcgttttt tatagaaaat tagatatacc gatcaaacag atgaagaatt tatatggaaa
    11041 aacattgacg gagttatatg agacacttga tgaaacagaa agaagaatcc ataaagaact
    11101 ggtggtgatg aaacaaaaac aaaaagagat aagggagcaa aaaaaacagt tgaaattgat
    11161 gatcgataca aacgaggaag aatttcctgt agaagagatc ccttttgact gtatgatctc
    11221 aacggagttt gaagatattg tggaaatcaa aaaatttcta cctaactatt caagctttgg
    11281 gatgatgagt atgccagctt ccagttcaca gacgatgtat ggatttttta tcgaccctag
    11341 tgaagtacat ttatttactc aagacgtgat ttgggagaaa aaagatacgg ctgtttatcg
    11401 tcgattctta ttgaaatccg agatgaacca tgcggaacgt aataatattt tagaaatcag
    11461 agaaagaatg tatgaaaaag gatggaaaac aggagaagtg atcggccagt atctattgac
    11521 gaatacggat gaaaacaata tcagaactga atattatcat gcatggatag aaatgaaaaa
    11581 ataggaaata tgtgtttaag ataactcgaa aatgatggtt gttttagtta taaagaaaaa
    11641 ttttctggtt cgattacggg tttaatgtgg gggattatta gtcgtggata gatagaattt
    11701 tagtttttta gaggaaatat gtctttaaaa atggaatggt tgcaattctt agcgatatat
    11761 acgatgtaat tgtcctctta aaaaagtatg gaaaaataat tgtattgggt gttatgtata
    11821 attcatggtg aactgagact aatgagaaga aaaattcata ttgttatgag tgagattgta
    11881 tcagatgaga atgataaatt aaataggtgc ataaaagcat tacacttaaa atacattgtt
    11941 gcttacatta ttacaatatg ttaataaggc aaaaataaat agatggattg aataaacata
    12001 ttttttaatc aaatagtccc tactgtgact ttcctcagct atcggtgata ctaccgatag
    12061 catttttttg cttataaaat aattat
//
LOCUS       DAZUMM010000078        11893 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000078 DAZUMM010000000
VERSION     DAZUMM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.080"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..461)
                     /locus_tag="ACG8E6_002145"
                     /pseudo
     CDS             complement(1..461)
                     /locus_tag="ACG8E6_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
     gene            complement(713..1072)
                     /gene="rplT"
                     /locus_tag="ACG8E6_002146"
     CDS             complement(713..1072)
                     /gene="rplT"
                     /locus_tag="ACG8E6_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFS5062535.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1125..1325)
                     /gene="rpmI"
                     /locus_tag="ACG8E6_002147"
     CDS             complement(1125..1325)
                     /gene="rpmI"
                     /locus_tag="ACG8E6_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFS5062536.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1397..1921)
                     /gene="infC"
                     /locus_tag="ACG8E6_002148"
     CDS             complement(1397..1921)
                     /gene="infC"
                     /locus_tag="ACG8E6_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFS5062537.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1965..2090)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2241..2873
                     /locus_tag="ACG8E6_002149"
     CDS             2241..2873
                     /locus_tag="ACG8E6_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS5062538.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2875..3780)
                     /locus_tag="ACG8E6_002150"
     CDS             complement(2875..3780)
                     /locus_tag="ACG8E6_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS5062539.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3865..4524)
                     /locus_tag="ACG8E6_002151"
     CDS             complement(3865..4524)
                     /locus_tag="ACG8E6_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS5062540.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            4840..5784
                     /gene="mvk"
                     /locus_tag="ACG8E6_002152"
     CDS             4840..5784
                     /gene="mvk"
                     /locus_tag="ACG8E6_002152"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFS5062541.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5777..6754
                     /gene="mvaD"
                     /locus_tag="ACG8E6_002153"
     CDS             5777..6754
                     /gene="mvaD"
                     /locus_tag="ACG8E6_002153"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFS5062542.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6767..7852
                     /locus_tag="ACG8E6_002154"
     CDS             6767..7852
                     /locus_tag="ACG8E6_002154"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFS5062543.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7849..8913
                     /gene="fni"
                     /locus_tag="ACG8E6_002155"
     CDS             7849..8913
                     /gene="fni"
                     /locus_tag="ACG8E6_002155"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFS5062544.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(9162..9965)
                     /locus_tag="ACG8E6_002156"
     CDS             complement(9162..9965)
                     /locus_tag="ACG8E6_002156"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFS5062545.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9985..10308)
                     /locus_tag="ACG8E6_002157"
                     /pseudo
     CDS             complement(9985..10308)
                     /locus_tag="ACG8E6_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
     gene            complement(10341..10478)
                     /locus_tag="ACG8E6_002158"
                     /pseudo
     CDS             complement(10341..10478)
                     /locus_tag="ACG8E6_002158"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            complement(10522..11861)
                     /locus_tag="ACG8E6_002159"
     CDS             complement(join(10522..11420,11420..11861))
                     /locus_tag="ACG8E6_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFS5062546.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT         3497 a         2464 c         2190 g         3742 t
ORIGIN      
        1 aaatacctaa tgacatcaag atcggcaaca caataactgc aatagaaatc actggtccga
       61 ttcctgtcat cgacatgaat ataacagcgg ttacaataca tagtaagctc atcgtgattc
      121 ttggcttgtc ccctccaagt tcaactacct ttcgaatcag ggtagctgcg attccgctat
      181 ctactagaac acgcccgaaa aaggcaccaa agaagacatt taccaagatc gatttggcgt
      241 actcagctgg ccccgtcgta tagatatgcg tcaacacatc aattactcct tgcccttcca
      301 tcgccgagtt tggtgaaaac gtgtttccga ctagcgctaa tcccatccat acagtagcca
      361 tgatgacaaa agccgaccat caagttgtag ccttttacac agtaccagac catgaaaaag
      421 aatgagataa ctaacaaaat accaataata atatcactca tgttattcct ccgtccattt
      481 tgtttttctg ataagcaaac taatatatca gtcaaactca ttctaactta tgtagttagc
      541 gctttcatta aaggatgacc ataaatgttc agttttcaat taaaaaacct taattatgtt
      601 attttagact tatacgttat tgtcctgttt catttgatgg aggattgtaa ataaaaaaag
      661 aggaaagaac ataacttttc gttatcttct ttcctcttta taattgttta gcttatttag
      721 ccaaagcttc ttttgcttgg tcagccaatg cagtaaatgc tgctgcatcg ttaacagcca
      781 agtcagctaa cattttacgg ttgatgtcga tttcagcaac tttcaaaccg tgcattaatt
      841 ttgagtagct caagccattc atacgagctg ctgcgttgat acgagcaatc cacaatttac
      901 ggaagtcgcg tttcttttga cgacgatcgc gatatgcgta gttgtatgaa ttcattactt
      961 gttcttttgc tgttttaaat aaagtgtgtt ttgaaccgta gtaacctttt gctaatttta
     1021 gcactctttt acgacgttta cgagttactg ttccaccttt aacacgtgcc atgtataatt
     1081 cctcctaata aaaataaatc ttcagattct gttatttgta cttcttattt catttttgct
     1141 agttgttggc gaatacgttt gaagtcgcca ctagaaacca tgcttgcttt acgcaattga
     1201 cggcgttgtt tttttgtttt gccgtggaaa cggtgacttg taaacgcgcg gaagcgtttc
     1261 aatccgcctt taccagtacg tttgaaacgt tttgctgatc cgcggtgtgt tttttgtttt
     1321 ggcatgacta tatttcctcc tcaaaatctt tcgttatccg actatcggct gtccaacagc
     1381 cagttagtca ttcaaattac ttactgtcgt ttttcggtgc cagcgtcaag aacatgctgc
     1441 gtccgtccat tttcgctttt tgttccactg ttgcaatatc cgcagtttct tcagctaagc
     1501 gattcaagac tttttgacca atttctttat gggtaatggc acggcctttg aaacggatag
     1561 aagctttcac tttatcgccc ttttccaaga acttacgtgc attacgaagt tttgtattga
     1621 agtcattcaa gtcgatcgta ggacttaagc gaacttcttt tacattgatc actttttgtt
     1681 ttttacgcgc ttcgcggtct ttcttttgtt gttcgaaacg gtatttaccg tagtccatga
     1741 ttcgcgcaac tggtggtttc gcaccagggg caactaacac aagatccaag tttgcttgtt
     1801 ctgcaatatt caatgcttct gcttttgtct taactcctaa ttgctcacca tctgaaccga
     1861 tcaatcgtaa ctctcgtgca cgaatgccgt cgttaaccat catatccttt gctatggtca
     1921 ttcacctcca aatattatga gagaaagaaa agcttaccga ataaacggca ataaaaaaac
     1981 gagttctctt acagaacccg cgcgtacttc acctaataga accagtgaaa actatctagc
     2041 ccagtgacat tatcaactaa ggcgagaagc gggtgcttct gcttttattt ctcaaccatt
     2101 atagtatagc aaaaactaat ctttttgtca atgtttttca tacagtttgt cttccttttc
     2161 tgaaaatgat tagattagca ggctttctag cagtgattcc cgcttctttg ctatactaaa
     2221 gaaaaacgag gtgaacccac atgaaaaaaa tcgaacctat tccaccttct cttcctcttc
     2281 ttacaaaggg agagttttat ttagaagatg aggagatctt ctctggtctt ctgatcaacg
     2341 gaggtaacca aagctatctc gatgccaaaa atatggtatt aagagagagt catatccaaa
     2401 aacttacgat gcaaaaaaca ggtttacgtc attttgaatg cagtaatgtc atttttgaaa
     2461 aatgtgattt ttccaatctg gagtggattg gtggcagttt ccaccaagtc gtgtttcatc
     2521 aatgtaaatt aacaggtacg aattttgccg aaagttatct tcgagactgt actttcactg
     2581 attgtattgc ttcgatggct tcctttagta gtacaaactt gaaagctgtc tccttcgatc
     2641 attgtcaatt agaagacagc gaattttatg aggtcacttg gaaaaacctt tttttgtctg
     2701 acaatcagtt aaccggttcg aactggtttc gaacttctct gaagggatta gactttacga
     2761 ctaatttctt cacaacaatc gctttatctc aagattcttt ggcgggacta atcgtcaatc
     2821 aagagcaagc acttgtgatc gcccaagccc ttggtttgca gatcaaagac tagatcaacg
     2881 aatccgccgc cacagtgcaa taagcatcca aatagataag accaaactga tgatcaaggc
     2941 gatttcccat ccgaaaacgg aatgggtaaa gggtaactgg agattcattc cgaagaatcc
     3001 tgtcacaata gttggaacgg tcaagatcag cgaccagacc gtcaaaaatt tcatcgtgtc
     3061 attcaagttg ttattcagca aattattata agtgccagag agctggtcta aaatctgcga
     3121 agctaagttc gtcatttcaa ctgcttgtcg tgcttcgatc agtgcatcgt ccaactcttc
     3181 tttttccttt tctgacaact tgcgataaat agccagtgcc ttgatttgtt ctaaaagtac
     3241 tacattttgt tttgttcctg taactaagta aaccaaaccg atctctaaat ctgaaagttg
     3301 cagcagattc ttgttcgttg ttttttctct taatttcaga ttaagccgct gacgttcact
     3361 attcacttcc tcgattaaag gaaaaaagga atcagaaatc aaaaacaatg tacgaaacaa
     3421 taaactgtat acactcatgt ctggcatccg ttccaaaagc gatcgaatta gtcgaatcac
     3481 gtactcggta ttcggcgtgg taaatgtaaa caaaccgtct tcttttaaaa taaaagcgat
     3541 aggactagtt tcataatgat tatcgacttt ttgctggtgc ggaacatttg atacaagcag
     3601 taacgcttca tcaaaagaat cgtactctac gcgcgcccgt tcatttttat ctagagaata
     3661 agtcagcatc tcgtcactaa tgccatattc ttcttttaag tttttaacag ctagcacatc
     3721 gttttgctgt acattgatcc aaaaattacg gtcttgatta aaatagtgtt tttcccgcat
     3781 actctatcct cctgacattt gtttccagta taaagaaaaa agtcgctcaa ataaaggttt
     3841 accctttatt tgagcaactg taaatcaaat ggatactttg gtttggcgaa tctctgctaa
     3901 tttttcttcg atcattttca agacttcttc ttttgcttga ggatcttcca caaaattata
     3961 gcggtctcca tcgatttgga tttttggact ttctttgtat tcttcatacc attgatcata
     4021 acgtctgttc aattctttgt aataatcgta tagacttggg tcaaaggaca attgttcata
     4081 cgaacgacca cgtttttcga tacgctctaa catcgtatca aaagatacac gaatatgaac
     4141 taataaatcc ggatgttttt tatgggcagc atagggaagt tcttccatca tgttttccag
     4201 cagttcatca tacacacaaa cttctgtttc tgttgctctt cctagatctg catttagatg
     4261 gaaaagcagt gaatcttcat aaatcgaacg gtccaataca ttattgtcat ctttcattgc
     4321 ttgtttgatg cttgcaaaac gtttattcaa aaaataaatt tgcagcaaaa aagcatactg
     4381 ttcagggttt gcataaaata agggcaagac ttcattatca tcaatcgatt cataaaatgc
     4441 ctgactatca aagtgttcag ccatcatttc tgttaaactt gattttccgg caccaattgt
     4501 acctgctaaa acgatcacgc tcatttacct ccagctcact tcatcactaa atattgtgtt
     4561 taactccaac cttttttaaa tataccccca atatctagta gtgtcaattg tattttttct
     4621 agtcaaagaa gaaaaaaagt caaaaaattc ttaagaaaaa agtgtttttt ctttagaaaa
     4681 cgtattctta gttttacttc tgagatggtt ttagtaaaat aaaggaggaa tgttttttga
     4741 gacccttagt ggacaaaaaa attcgtgaag cagcgcaact cgttttgggc gctttatttt
     4801 ttgcttaaaa atcactttta cgtgagaagg agttagatta tggcaaacta tggccaagga
     4861 gaatcaagcg gaaagatcat attgatgggc gagcacgcgg ttgtttatgg agaaccagcg
     4921 attgcctttc ctttctatgc aacaaaagtc accgcattcc ttgaagagct ggatgcaatg
     4981 gacgatcaac tggtttcttc ctactattca ggaaatttag ccgaagctcc tcatgcatta
     5041 aaaaatatca aaaaattatt cattcactta aaaaaacagc atgacatcca aaaaaacttg
     5101 caactgacca ttgaaagcac gattcctgct gaacgtggaa tgggatcaag cgctgcagtc
     5161 gccacagcag tcactcgtgc tttttatgat tacttagcat ttcctttgtc tcgtgaaata
     5221 ctattagaaa atgtccagct ttcggaaaaa atcgcccacg gtaatcctag tggaatcgat
     5281 gcagccgcta ctagcagctt gcagccgatt tattttacaa aagggcatcc ttttgactac
     5341 ttttctttga acatcgatgc ttttttgatt gtcgctgata caggaatcaa aggacaaaca
     5401 agagaagccg tcaaagatgt cgctcacctc tttgaaaccc agcctcatga aactggacaa
     5461 atgatccaaa aattaggata cttgacgaag caagcaaaac aagcaatcat tgagaattca
     5521 ccagaaacgt tggcacagac aatggatgaa tcacagtcac ttctggaaaa gctgacaatc
     5581 agcaatgatt ttcttaatct attgatccaa acagcaaaag ataccggggc attgggtgcc
     5641 aaattaactg gcggtggacg cggtggctgt atgattgcat tagcacaaac aaaaacaaaa
     5701 gcccaagaaa tcagtcaagc acttgaagat gcgggtgctg ctgaaacttg gatacaagga
     5761 ttaggagtac atacctatgt ttaaaggcaa agcacgcgca tataccaaca ttgctctaat
     5821 caaatattgg ggtaagaaaa atgaagaact catccttcca atgaacaaca gcctttcgtt
     5881 aacattggat gcattttata cagagacaga agtcatcttt tctgatagct atatggtaga
     5941 tgaattttat ctagatggca ccttacaaga cgaaaaagca acaaaaaaag tcagtcagtt
     6001 tcttgacctt tttcgtaaag aagccggcct ctcactaaaa gcttcagtaa tcagtcagaa
     6061 tttcgttcct accgcagctg gattagcctc ctctgccagc gggctagctg ctttagcagg
     6121 agcttgcaat actgctctta agcttggatt agacgatctc tctctttcaa gatttgctcg
     6181 acgcgggtct ggttcagctt gccgaagtat tttcggtggt ttcgttgaat gggaaaaagg
     6241 ccatgacgac ttaagttctt acgctaagcc agtcccttcc gattctttcg aagacgattt
     6301 agcaatggtt ttcgttttga tcaacgacca gaaaaaagaa gtgtccagca gaaatgggat
     6361 gcgtcgaaca gtcgaaacat ccagttttta tcaaggctgg ttagattccg ttgaagggga
     6421 tctatatcaa ttgaaacaag caatcaaaac aaaagatttc caacttctcg gagaaacgat
     6481 ggaaagaaac ggactaaaaa tgcatggaac gactttagct gcccagcctc cattcactta
     6541 ctggtctcct gacagcttaa aagcgatgga tgcggtgcgg caattgagaa aaaaaggcat
     6601 tccatgctac tttacgatgg atgctggccc taatgtcaaa gttttagtgg aaaacagcca
     6661 tttatctgag gtacaagaaa catttaccaa attatttagc aaagaacaag taattactgc
     6721 ccatgctggt ccaggaattg cgattattga ataaagggag atttttatga ttgaagtatc
     6781 tgcaccaggc aaactctata ttgccggaga atatgcagtt gttgaaacag gacatccagc
     6841 agttatcgct gcagtcgatc aattcgtaac agtcactgta gaatccgcac gaaaagtcgg
     6901 aagtatccaa tctgcacaat atagtgggat gcctgtacgt tggacaagac gcaacggaga
     6961 attagtttta gatattcggg aaaatccttt tcattatatc cttgctgcta ttcgtttgac
     7021 tgaaaagtat gcacaagaaa aaaacattct tttatccttt tatgatctga aagtaacaag
     7081 tgagttagac agttcaaatg gccggaaata tggattgggt tctagcggtg ccgtgaccgt
     7141 tgcaacggtc aaagccttga atgtttttta cgcgttgaat ttatctcaat tggagatttt
     7201 caagattgca gcactagcca atttagcagt tcaagataat ggttcctgcg gcgacatcgc
     7261 tgctagctgt tatggtggct ggatcgcttt ctcaaccttc gaccatcctt ggctccaaga
     7321 acaagaaact cagcattcta tcagtgagtt acttgccctg gattggccag gtctatccat
     7381 tgagccattg attgctcctg aagatttacg tttattgatt ggttggacgg gtagccctgc
     7441 ctctacttct gatttggtcg atcaagttca ccgttcgaga gaagacaaaa tggtggctta
     7501 tcagcttttc ttaaaaaaca gtacagaatg tgtcaacgaa atgatcaaag ggtttaaaga
     7561 aaataatgta acgttgatcc aacagatgat tcgaaaaaac cgacaattac tgcatgattt
     7621 atctgcaatc actggggtcg tcatcgaaac gcctgctttg aacaaattgt gtaatttagc
     7681 tgaacagtat gaaggagccg caaaatcttc tggtgcaggt ggtggcgatt gcggaatcgt
     7741 aattgttgac cagaaatctg gcatccttcc tttaatgagt gcatgggaaa aagcagaaat
     7801 cactccactg ccgttacatg tctatagcga tcaaagaaag gaaaaccgat gaatcgaaaa
     7861 gacgaacatg tttcactagc taaagccttt catgataaac aaaaaaacga atttgacttc
     7921 gtacggatca tccataaccc tttgccgcaa atagcagttt ctgatgtcga tctaagtaca
     7981 caggcagttg ggtttacact aagcagccca ttttatatca atgcgatgac tggcggaagc
     8041 gaaaaaacaa aaaaaatcaa ccaagatctt gcaatcgtcg caagagaagc agatttgatg
     8101 attgccactg gttcagtcag cgctgccttg aaagaccctt ctttagccga tacttatacg
     8161 attatgcgac aagaatatcc tcacgggaaa atcattgcca acatcggtgc agggacttct
     8221 gtcgaaagag cccaagaagc tatccgattg tttcacgcag atgccttaca gattcatttg
     8281 aatgcacctc aagagttggt tatgcctgag ggagaccgtg attttaccaa ttggaaagta
     8341 cttattcaag agactcaaac agccattgac gtccccctca tcgtaaaaga agtcggtttt
     8401 ggcatgacaa gagaaacgct caatgatcta gctgctttag gagttcatac agttgatatc
     8461 agcggtcgaa gcggaacaag cttcacccag atcgaaaacg cccgtcgctc caagcgggaa
     8521 ctgagttatt tagctgactg ggggcaatcg acagtctctt cattgcttga agcaaatgaa
     8581 gcagacactt ctatggaaat cctagcctcc ggaggtatcc gcaatgccta tgacatattt
     8641 aaagcccttt gtctcggcgc aaatgcagtg ggtacttcag gaaccgtact gacgcatctg
     8701 atgaatcacg gcgtagaaga aactattata ctgatgaagc aatggcaaga agagcttcgt
     8761 ctgctttata ctatggtcgg cgcaacgaat accgcggccc tacaccaaca atcactgatc
     8821 ttttccggac cagtgaagga ttggtgcgaa gcaagaggga tcgacttagt gaagtatgga
     8881 agacgcacag aaaagagcgt gggacaaaag taaagaacac ttttatccca cgctctaaac
     8941 acgtataaac ggcggaagca aaagcaactc cttcggaaat aagctgaaat tcacaaaaat
     9001 ttgaaaagca attttcgtga attccttctt atttcttgga gttaaacgct tctgtcccag
     9061 cctctttttt cgaataaatg atgtttaatc agctgtcctt cggtattcgt tcaagccgct
     9121 gatttcacaa cctctccact aaacggtgta ctaaagctct tttaaggttg ttttccgata
     9181 taggcaagta tgcctccgtc cacatacaag acatggccgt taacgaaatc agatgcgcta
     9241 gaagccaaga agacagatgg tccagccaaa tcgattggct cgccccagcg tgctgctggc
     9301 gtacgtccaa cgataaattg atcgaatgga tgacgttcac cgttttcttg tgtttcgcgt
     9361 aatggagcag tttgcggtgt agcaatataa cctggtccaa ttccattaca ttggatattg
     9421 tattgaccgt attcagaagc gatgtttttc gtcagcattt ttaatccgcc ttttgccgca
     9481 gcataagcac tgaccgtttc gcgtcctaat tcactcatca tggagcagat attgatgatt
     9541 ttaccgccgc ctttttcgat catatcccga ataactgctt ttgaaacgat gaatggggca
     9601 tttaaatcca cgtcgattac ttggcggaag tctgccgcat ccatttcgat catcggtgtc
     9661 cgtttgatga tccctgcatt gttgactaga atatcaactg aacctacatc ttctttgatc
     9721 tgttgaacca tcgcttgaac cgcttcttca tcggtcacat cgcagacata accttttgca
     9781 tcgatgcctg cttctttata atgcgctata ccttcatcaa ctgattcttg attcaagtta
     9841 ttaaatacga tttttgctcc tgctgtagct aaagagcgtg caatctcaaa tccaatacca
     9901 tagacagcac cggtgactaa tgctactttt ccatctaaac gaaacatatc catactaaat
     9961 tccatcattt tctctccctt atgcttattt taattgatcc atttgcacca tatccatgtc
    10021 tgtataggtg atattttctc cgcacattgc ccagatgaat gagtaattgc ttgtacctac
    10081 acctgaatgg atcgaccagc ttggcgaaat aatggcttgt tcattgtcta cgactaaatg
    10141 ttttgtttca tctggtttgc ccatcatatg gaacacacgt gtatcttcat tggcataatc
    10201 aaaatagaca taagcttcca tacgacgttc atgtgtatga caaggcatcg tattccaagc
    10261 acttcctggt tctaaaatcg tataacccat ttataaatga cagctatcgc tgaagcttct
    10321 actgctgtaa atatccccgt caagatacca ccgataatga tgacaatcaa tagaaggcta
    10381 ggaatcgcct cttttattat ttttccagca tgagccatct gtcctttttc gacaacaggg
    10441 tatttattct ttttagcaac gatgaatgct actagcatga ttcctagcgc ccacaaagtg
    10501 aactgaaccc caaaagttga actatttaat ggactgtttc cgatattcta ctggagatag
    10561 tccatttaac tttagtttta tccttttgtt attataccac ctaatatact catttaattt
    10621 actttgaaat atctcaattg atcggaattt ttctcgataa aaaaactctg atttaagcac
    10681 accaaaaaaa ttttctatta cagaattatc taagcaattt ccttttctag acatgctttg
    10741 aataatgtta ttctctttta attttttttg atattgtggc atctgatact gccatccttg
    10801 atctgaatgt agaatcagtg aaccctcagt tccctttttt ttaattgctt gttgaagcat
    10861 ttcttcaatc agtttatatg ttggactagt tgatatacta taactaatta tttctccgtt
    10921 aaataaatca agtataggag atagatagat ttttcttcct tttatcttga attcagtgac
    10981 gtctgtcacc cattttttat tgggtgtatc tactgaaaaa tttcgtttta acacattctt
    11041 ggcaattttt cctactgttc ctttataaga tttatatcgc tttattcgga tttgacaggt
    11101 aagtcccatt tgggacatta attttctaac tgttttatga ttgattgtat atcctttcat
    11161 ctttaacgct aaagtgactc tacgataacc ataagagttt cttgattctt ttacaatcgc
    11221 tgtaatttct tgctttatct tgctatattt atctggctta tctaattttt ttacccagta
    11281 ataataagtt gacttcgcta attgtgcaat tgaaagtaat agattcaatt taaattcttt
    11341 tttgagctga aggattgttt taaccttgat ttcttcttgc tcaaatcttg ttcttgaatc
    11401 aaggtttcta acttttttaa atatgcgttc tctgctctta aacgaataac ttcttcttca
    11461 agagactcat ctttaagttt tttaggaatg tttagcttgg aattcatact aatttttctg
    11521 cccctttttt ggctctcaag tgaagaagca ccgccttctt catattgctc tatccattta
    11581 cttagagttc tatttgaacc gatattaaat tttttagcag tttcttggat agaaagacca
    11641 tttgtttcca tatattctat aacatcaagt ttaaattttg tagtgtagct tttgccacct
    11701 ccaaccaagc cttcccaacc atgatagtta taaatcctta cccaatgtct aaccagtgta
    11761 cgatttattt gatatttatg tgcaagatat ttgtagccgc cttcgttatt taaatagtct
    11821 gaaactactt ttttcttaaa aacaaatgta tatttccgca aaaaaagcac cccttttaat
    11881 tagatttcta gtc
//
LOCUS       DAZUMM010000079        11850 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000079 DAZUMM010000000
VERSION     DAZUMM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.081"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..970
                     /gene="thiD"
                     /locus_tag="ACG8E6_002160"
     CDS             155..970
                     /gene="thiD"
                     /locus_tag="ACG8E6_002160"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFS5062547.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1299..2750)
                     /gene="cls"
                     /locus_tag="ACG8E6_002161"
     CDS             complement(1299..2750)
                     /gene="cls"
                     /locus_tag="ACG8E6_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS5062548.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3091..5757
                     /locus_tag="ACG8E6_002162"
     CDS             3091..5757
                     /locus_tag="ACG8E6_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5062549.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6121..6873
                     /locus_tag="ACG8E6_002163"
     CDS             6121..6873
                     /locus_tag="ACG8E6_002163"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5062550.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(6895..8049)
                     /locus_tag="ACG8E6_002164"
     CDS             complement(6895..8049)
                     /locus_tag="ACG8E6_002164"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFS5062551.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8234..10675
                     /locus_tag="ACG8E6_002165"
     CDS             8234..10675
                     /locus_tag="ACG8E6_002165"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFS5062552.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(11005..11709)
                     /locus_tag="ACG8E6_002166"
     CDS             complement(11005..11709)
                     /locus_tag="ACG8E6_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062553.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         3821 a         2105 c         2504 g         3420 t
ORIGIN      
        1 tattttctga tttattgcaa atgttttttc aaataaattt gattaccaag aaaaggtagt
       61 ggtatattta ctgagggata ctttgaaaaa catatgatag aaacagtaac agtattatga
      121 aaaatctttt tataaaaatg gaggaatcga tttgatggaa gatcagtatg tacaagcaat
      181 gacgatcgca ggatcagata gcgatggaag cgcagggatg caagcagata tgcatacatt
      241 ctttacaaga aatgtttacg gtgtttctgt catgaccgca tgtgttgcag gtaattctta
      301 cggaatcgga gcaagtgtga cattgccaac agattttatc gacaaagaat ttgaactgat
      361 tgcgaaggac tttcaaatac gtgcagctaa aacaggtatg ctagcagatt caaagttgat
      421 tgagacagtt gtaaaaaatt accaaaaata tgattttgga ccattagtcg tggatcccgt
      481 aatcgtaacg aaacatggaa atcttttact agaagaatct gctttgcaaa gcttgaaaga
      541 aaagcttgtc cctttggccg aagtactgac acctaatttt tatgaagcag aaaagctgac
      601 tggacggaca ttagcagctg aagaagattt tgtgcaagca gcaaaagaac tgcaagatat
      661 gggtgcaaaa aacgttatga tcaaaggaca tcatgctaaa aatgcgcagc aagaaacagt
      721 caatgactac gtattgttcg aatcaggaaa atcattttgg ttaaatgcac cattttatcc
      781 taccgaccgg atcaacggaa ctggcgattc gctatctgca tgtattgcag ctgagttagc
      841 gaaaggaaaa acagttgaac aagcgatccg attggcaaaa gactatgtaa accgtgcaat
      901 tcgtgaagaa ataaatgtag ggcacaaata tggccccatc aatcattggg cagctggtga
      961 attagagtaa aagaaagaag ttgtgaaaaa gttgccctgc atcaagtaat aagaacagcc
     1021 aagtaaaaac agcttcttat gtttttaact tggttggact tattaccgca gatgcaagct
     1081 tttgaacacc gtttaagaaa gaggtcgtga gcctgccgtc cagctccgag tcacaaggaa
     1141 caagttcaaa aaacagcttc tcatgtttgt tgaatattgt gacttgtgac cgaggagttg
     1201 gcaggagaac accgtttgtt cggaagaagg gactgtgaca agatttttgt cacagtccct
     1261 ttaatcttag tatatttttc tctacttgaa taaaaaaatt ataggattgg agaaaataat
     1321 cgagaaattt gttgcttgaa taatagccag ttagacattt ccttgactgt ttcagttgtc
     1381 atggatgaac aatgtgtctc atcttctttg aactgagtag caagttcttc taaaaactca
     1441 ggatcgtaaa tgacagcact tgtttcaaaa ttcaatcggt agctacgaat atcttgattt
     1501 gctgaaccta ccatgcagat ttcatcatcc attatcaatg tttttgcatg gaggaagccg
     1561 ttctcataga caaggatttg tacattttcc ttgatcagct gccgagcata atattgtgtt
     1621 gctcgataaa taaaaggatg atccggtttg ttgggaatca tgattttcac atctacacca
     1681 gaggcagttg cgatttttaa agcagcaatg acactttcat caggaacaag gtaaggtgtc
     1741 tgtatccaaa cacgtttttt agcagaagta atcaatttga taaatgaaag cttgatttgt
     1801 tccctttcgt tattcggtcc gcttgaaaca agctggatgg atgtatcagc aagcttagat
     1861 tcatctcgat ctgctttttt aaagaaataa ttcaaatgat agccgacctt tttttcttca
     1921 ggtaccgaga cgttccagtc tgttaagaag cgaagctgga gcaaagaaga agcggcaccg
     1981 aaaatccgta tatgcgtatc gcgccaatag ccgaattttt ttgttgtatt tacatattga
     2041 ttggcaatat tgaaaccacc ggtataacta atctttccat cgatcacaac gatttttcgg
     2101 tgatcatggt aattcaatcg gaaacgaagt aatgcccttt gtgaggtaac aaatgtatgg
     2161 acatgtccac catttttgat tagacgattg aaatcttttg cttttgtgcc atgagagcca
     2221 aatgcatcat ataatattcg aacttccacg ccttctgcgg ctttttcttc taaaacatgt
     2281 aagacttgct ggccgatatt atctgtcaca aatgcgtaat attcgatatg gatcgaatgt
     2341 ttggcttttt ttagatcttg aagcagtgaa tccaatttct cttgtccgtc tgtgagaaga
     2401 gtgacagaat tcattcttgt cagcggcata cgatttaatg aagagaagaa gtcaacaaat
     2461 tgctgtttgt ctttatcgcc catgtcagga tcgtaatgtt caatggtatc tcctctaaaa
     2521 gactgaaagt tttctaattc tttcaagtca ctttgtcgga gatagaattt tttcttatcc
     2581 gttaatccac gtccgaaaaa taaatataat acaaagccca ccccaggaag ggcaaacaat
     2641 actaagagcc atgcccaaat ggctgctaca tctcggggtt tgatcaaaat agccaccaaa
     2701 gcaataagtg catttaatag ataaagggcg gttataatac tagataccac aaaaatgttc
     2761 ctccttacat acaataacat tagtatagca tgatagaaga aagaagccta ccaaggtgaa
     2821 tatacttttc actcctttca tgaaaatgta ggtaaatcgg gctaaagtag ttgctttttg
     2881 taggttaata aggtaggata ttagtacagt tgaatatctc cttaggggag taaccggcaa
     2941 cgtttgttgc gataatatca acagtaagtt aaccaaatgg ttttctggta ttgtatgaaa
     3001 tggtgagacc taagacaaat gcgtatttgt cttaggtctc ttttttttca gacaggacta
     3061 gggccaagat caaaaaatgg gggatgaaat ttggaaaaca aggaacaagc acaacaagcg
     3121 gtctataaag aaacgatcga ctcgttgatg cagcaaacgc agagctcaac cgatggattg
     3181 agcaccacgc aagcgaagga aaaattagaa agagatggct tgaaccagct aaaagaaaag
     3241 ccgaagaagc caacttggaa attgttctta gaaactttca aagatgcaat ggttatcgta
     3301 ttgctgattg tcgcactgat tcagatggtc atgggtgact acatcgaatt cttagttatt
     3361 tttgcagtat tgatgttgaa ttctataatc agtgttgtcc aaacaaaaaa agcagaaggt
     3421 tctttagaag cgttgaaaaa cctttcagcg cccgatgctc gagtgattcg tgatggtaag
     3481 gaaaaaacga ttccggcaaa cgaacttgtg gtaggagata tcgtgttgtt agacgcaggc
     3541 gattatgtac ctgctgatgg acgtttacta gatgcgggat cattaaaagt agatgaaggt
     3601 atgctgacag gagagtcgac ttctgcagaa aaagaagtaa cggatatcac aagtacggta
     3661 ccaatcggag atcgtacaaa tatggtattc agtggaacga tcgtaactta cggacgcggt
     3721 aaattcttag tgacttcaac tgggaataat acggagatcg gacaagttgc tggccttttg
     3781 gaatcaacaa ctgagcaagt cactccgtta caacgaggtc tggataaatt cagtaaaaaa
     3841 ctaagtttgg ctattttggc attgtcttta ttgattttag gtatccaact gtttcgtatt
     3901 tatcttggag aaggtacagg agatatgact gcagatatcg tcagcgcagt gatgtttgcc
     3961 gtagccgtag ccgtagctgc tattccagaa gcattacagt ctatcgtaac aatcgtgtta
     4021 tctttgggaa cgaataaaat ggcaaaacgt catgccatta ttcggaagct tccagcagtt
     4081 gaaacacttg gttcgacaag tatcatctgt acagataaga ctggtacatt gacacaaaac
     4141 aaaatgacag ttgttgacta ctttttaggt aatggcgata ctggggattt cacaaatgac
     4201 ccttcaaaat ggacagctga cgaacgtcgc ttgatcgaaa tcagtgtttt agcgaatgat
     4261 gcatcgatca gtgaagacgg tactaaattg ggcgacccta cggaagtagc gtttatcgac
     4321 tttagtgaaa aattaaacca gccttatcag gaaatccgaa acaactaccc aagacaagca
     4381 gaacttcctt ttgactcaga tcgaaaatta atgtctactg gacacacaat tgataacaaa
     4441 aattacttgt ttgttaaagg cggaccagat atcgtcttcc aacgcagtaa aaaagtattg
     4501 ctaaatggtg aagtcgttcc gatgacggat gaattgatga aaaaattcca agagcaaaat
     4561 gaagcttttt caacacgcgc attacgtgta ctagcttttg cttaccgtcc acttgataat
     4621 tttgatttat catttgaaga tgaagacgat ttgatccttg ttggtttgct tgctatgatc
     4681 gatcctccac gtgctgaagt gactcaggcg gtaacagaag caagaagtgc aggaatcaaa
     4741 acaatcatga ttactggtga ccacaaaaca acagctgttg ctatcgcaaa acaaatcggg
     4801 atttttgaag aaggaaacct ggctttgacc gggacggaac tagatgaatt gacagaagat
     4861 caactaaacg aagttttaga aaaagtcact gtttatgcac gtgtttcacc tgagaataaa
     4921 attcgtatcg ttcgtgcttg gcaaaataaa ggtcatatct cagctatgac aggtgatggt
     4981 gtaaatgacg caccagcatt gaaacaagca gacatcggaa ttgctatggg aacgggtaca
     5041 gatgtggcaa aagatgcagc agcgatggta ttgacggatg ataactttgc ttctattgtc
     5101 agcgcagtag aagtaggacg taatgtgttt gataatatca agaaagctat ttcttatcta
     5161 tttgcaggaa atttaggtgc gatcattgcg atcatctttg ccttagttgc tgactggtcc
     5221 aatccattta cagcattaca actgttgttc atcaatttag tcaatgattc actgccagca
     5281 attgcattag gtacagaaaa agctgaacct gctacgatga accgcccgcc aagagatcca
     5341 aattcaggaa tctttacgga caaaacattg atttctgtaa cgtatcgtgg tgtgctgatt
     5401 gggattgctg ttatctttgc tcaatggctt ggcatgcagc attcacctga attcggtgta
     5461 gccatgtcat tttcaacatt gatttggtgt cggacattgc aaacattccc agcacgttca
     5521 aacacacaaa cagctatcca agcagggttc ttctctaaca gaagtgtctt actagctgta
     5581 gttgtatgta gcgcgcttta ctgcttaaca atgatcccag gactgaaaga agtcttctct
     5641 atcccatcag catttggatt gacagaagtc tttacatgta tgggaatcgc acttgtgaca
     5701 attgttttga tggaaatcac taaattgatc attgtacgag tacaaggaaa taaataaaac
     5761 gaatcaacga ggttgtgagc ctgccgttta attctgagtc acaaggtgca atttcaacaa
     5821 acagcttctc atgtttgttg aatattgtga cttgtgaccg aagagttggc aggagaacac
     5881 cgtttaagga agaagttgta aaatcagcat tttgagctaa tactgagggt catctgattg
     5941 gacactgttt attcggaaag agggactttg acacgacttt ggtcacagtc ccttttttta
     6001 tttatgatag acggaaagcc aatcatttta ttattcatca aaagagatag aatagataaa
     6061 agaggagaat gatttattcc gaaaagtgaa aacatataac aaaaaattag aggaaatcaa
     6121 atgaatgaaa aagaatggga tgaatttgct gaagaatatt atgaaatcca gcaagaatcg
     6181 caagtaacta ttgctgaaga tgtaaaaaat tatctgaaaa cggagaaagt cctgcctgct
     6241 gaaacagcag cagacgttgc tggaggatct ggcagatttc tttccttagc aaaagaaatt
     6301 aatcgctatg atttgatcga tttttcttca ggaatgctag aaaatgcaaa aaaagaagca
     6361 gaaaaaaatg agttgccaaa tgtttccttt atcaaacagt cgttagaaga atttatccaa
     6421 tcaaatgaga catatgaaat ggtttttaca gcagcaaatc cagcattggt acatcttatt
     6481 caattagaaa aattgatgaa aaagtcaagc aaatggtgcg tgataatcag agtagtggaa
     6541 tcaatcgatg atgtttttta tcctatcgaa aagcaactag gaatgataga agaagatcct
     6601 aatacatcgc cagttgtaat ggatcaattt gaaacatact taattgatga aggattcaaa
     6661 gtgaagaaaa aagattttac ttattttaca caggaatcaa ttactgaaga attgatgaag
     6721 ctatactatg acgcgtacgc aaaagatcct gttgtgacca ggtacattcg caaactattc
     6781 agtgaaaaac gagaaatcct tagtacgact aagctgacct atagactgct cctttggtcc
     6841 gcagataaaa aaagaacctt tcacgactcg tgaagggttc tttttatgat tttattatat
     6901 tttgtagtaa cgaatatcat tttcgatctt tgtaatcgaa aaaggtacat ctgaagtata
     6961 ttctgcacat tctccgtgtt caggaagcgt ctcactgaaa atcgtttcat attcgtcaac
     7021 agtgatccgt gttcgagaat ctagcatctc ttgatgagat tgtgcgaaga gatattcttg
     7081 atagttttct tccagataac ctgtaaaaaa ttcacttact gcaccagaac cgtagctgaa
     7141 tagaccgatg cgatctcctg gctgtagtga tttcgaattt tccagtagag aagttagccc
     7201 caggtataat gaaccagtgt aaagattacc gattcgtcgg ctgtaacgga tgctttcttc
     7261 atagcgagcc ataagacgtt cctgattttc ttcgtctgtt tggtctaata cgctttgcaa
     7321 tgcctttttt cccatcttag tatacggaat atggaaagca atcgcctgat aatcttcgag
     7381 tcctcgaccc gacaattctt tatgtcgatt ccaaactttt tggaatgatt cgatatacgt
     7441 agaattagat aaaggaccat caacaacagg aaattcgcta taatctggac gccagaaatc
     7501 atagatatct tctgtcagaa atacgctgtc gtcttcaatc gataaaatac gcgggttttg
     7561 agtgatcatc atcgcaacag caccgacacc ttgcgtcact tcaccaccgc ttgccaagcc
     7621 gtaacgagca atatcacttg ctatgactag tacttttcgt tctggatgat ttttgacata
     7681 ttctttcgcc atgtgcaaag ctgcggtgcc accgtagcaa gcctctttta cttcaaaaga
     7741 acgagcaaaa gattggattt tcagtaaatg gtggataatt accgcactcg cttttgaatg
     7801 atcaatcccg gattctgttc cgacgatgac catgtcgatt agctcacggt ctttttctgt
     7861 tacgattttg ctggcagcat ttgctcctag tgtaatgatg tcttcatttg cacgattgac
     7921 tgccatttgg tcttgaccaa tccctatatg gtattttgca ggatcatccc cacggctttc
     7981 tgccaactct gtcatatcta aatataaatt aggaataaaa aaggaaagac gatcaatccc
     8041 tattttcatt tcaacacctc tttacttatg ataaaaaact tactttacta aggtatcata
     8101 aaaagattaa aagaaatata aaaaagagaa tatttaagga ttgttaaaat ttaatcttag
     8161 aaaaaaataa ctggaacaag agctatttgt ttttacagca aagaatggta tatttttgta
     8221 ggaggtggct tacttgaaag aagtcgttat gatagatgct gcaagaacgc cgataggaaa
     8281 gtatcgcgga agtttaagtc catttactgc agttgaatta ggcacacttg tgactaaagg
     8341 attattagat aaaacaaaac tgaaaaaaga taaaatcgat caagtaatat ttggaaatgt
     8401 cctgcaagct ggtaatggtc aaaatgttgc cagacagatt gctttgaaca gcggtctccc
     8461 tgtagatgtc ccagcaatga cgatcaatga agtttgcgga tctggtatga aagcagtgat
     8521 cttagcacgc cagctgatcc aattaggtga agcagaactt gtgatagccg gcggaacaga
     8581 gagtatgtct caagcaccta tgctgaaaca gtatcagtca gaaacaaatg aatatggtga
     8641 accaatttcc agtatggtca acgacggatt gactgacgca ttttcaaatg cacatatggg
     8701 attaaccgca gagaatgttg caacacaatt ttctgtgagc agagaagagc aggatcgcta
     8761 tgccttgtcg tcccagttga aagcagcaca tgctgtcgaa gccggtgtat tttctgagga
     8821 gatcatccca gtcaagattt ctgatgaaga cgtgttatct gaggatgaag cagttcgtgg
     8881 aaatagtaca ttggaaaaac tgggcacgtt acgtacagta ttctcagaag aaggaactgt
     8941 aacagcagga aatgcttccc cgttgaatga cggtgcctct gtggtgatcc ttgcatccaa
     9001 agaatacgca gaaaataata atctgcctta tttagcaacc atcaaagaag tagcggaggt
     9061 cggtattgac ccaagtatca tgggaatcgc tccaatcaaa gccattcaaa aattgacaga
     9121 tagatcagga atgaatttat caacaatcga tctttttgag atcaatgaag catttgcagc
     9181 ttcttctatc gtagttagcc aagaactgca gttagatgaa gaaaaggtca atatatatgg
     9241 aggagcaatc gcattaggcc atccaattgg tgcaagcggt gcacgtatct tgactacatt
     9301 agcttacgga ttgcttcgtg aacagaagag atacggtatt gcttcattat gtattggtgg
     9361 aggtttaggc ttggcagtct tgttagaagc gaatatggag caaacacata aggatgtgca
     9421 aaaaaaaaag ttttatcagc ttacgccatc tgaacggcgc agtcaattaa tagaaaaaaa
     9481 tgtattgaca caagaaactg cgttgatttt tcaggaacaa acactttctg aagagctctc
     9541 cgatcacatg atcgaaaacc aagtgagtga agtggaaatc ccaatgggga tcgcgcagaa
     9601 ttttcaaatc aatggcaaga aaaaatggat tccgatggca acagaggaac catctgtcat
     9661 tgcagctgca agtaatggtg caaaaatctg tggaaatatc tgcgcagaaa cacctcagcg
     9721 gctgatgcga gggcaaatcg ttctatcagg aaaatcggag tatcaagcag tgatcaatgc
     9781 agttaatcat cgaaaagaag agctgattct ttgtgcaaat gaaagttatc cttcaatcgt
     9841 gaaacgcggg ggcggtgttc aagatatctc tactcgggaa ttcatgggga gtttccatgc
     9901 atacttatct attgattttt tagtggatgt aaaagatgca atgggcgcta acatgatcaa
     9961 ctcgattctt gagagtgtgg caaacaagtt acgggagtgg ttccctgaag aagagattct
    10021 attcagtatt ttaagtaatt ttgctacaga atcattggct tctgcctgtt gtgaaatccc
    10081 attcgaacgc ttagggagaa acaaagaaat cggtgaacaa attgccaaaa aaatccaaca
    10141 ggcaggggaa tatgcgaagc ttgaccctta ccgtgcagca acgcacaaca aagggatcat
    10201 gaatggtata gaagctgtag tggcagctac aggaaatgat acacgtgcgg ttagcgcttc
    10261 gatccacgcc tacgcggcaa gaaatggatt ataccaagga ttaacagact ggcagatcaa
    10321 aggggataaa ctagttggaa aactaacggt gcctttggct gttgccacag taggcggagc
    10381 ttccaatatc ctgccaaaag caaaagcatc tttagctatg ttagatattg attcagccaa
    10441 ggaactagca caagtaattg ctgcagtagg gttagctcaa aacctggctg ctttgcgtgc
    10501 attagttaca gaaggaatcc aaaaaggcca catgggactt caagctcgtt cattagcgat
    10561 ctcaattggg gctattggtg aggaaatcga gcaagttgcg aaaaaattac gtgaagcaga
    10621 aaagatgaac cagcagacag ctatacaaat attggaaaaa atccgggaaa aataaaaaag
    10681 caatatcttt agaagaataa agcaagattt acagttattt tatttactgc agatcttgct
    10741 tttttctcta tcagaaaata tgtttattat tagaattttt tatagttaag gttaggcatc
    10801 ttaagaaatc aggcaactcc aagatatctt caggtagaag agaagtcgcc taacattgta
    10861 ggatcatagg tgatttgttg gacaggtcaa gctgatattt gagcctgatg ttagaaggaa
    10921 tttcttttta cttgctgtag aaattattat atgactaaaa aggctgggat tttaaatccc
    10981 agccttttat gacaaataga atttttatcg atcagtaatc gctgtcgctg caaattgttc
    11041 gatcaaagga atcaaagtca atttgacttt gtatttccgt tcgagtgctt tttcgataac
    11101 gaactcattt tcagtagtca tagaaatagt tgctaattta cgaggttcat cagcattttt
    11161 ttcattaata actacttcat gttcattttg ttctgtgtaa aatttaggat actcagcaac
    11221 ggcttttgaa aaatcagcaa aacttaaagc ggcattcgta tcagttgagc gtgtattttc
    11281 agttgtgtcc gcttttggtt tttccgtttt ttcttctggc acactgactg tttcttctgt
    11341 aactggtgtc gcaacagctt gcgcagttgg ttcatcgttt ttcaatcgtt gatcattcca
    11401 gtaagacacg atttcaataa gaagattgtc acggaatcgt tcgaaaatcc cgttttctcc
    11461 tgaacgaatt ctctttaata cagtattgaa gttgtcatcg attggcacgt tgatccgtgt
    11521 atcttcattt gttttcccag ctaatccgat cgttaagatt tgacggtttt tgggatcaag
    11581 catgacttgt aacggcaaac cataacgtcg caagctctct tcttctagag aacggaataa
    11641 ttgtcctctt acaccatcca atgaaggacg gaattgattt aataattttt gtttctgttt
    11701 tttcttcata tgttctctcc ttcgctcata ccctttcgat tataagaaaa aaacagccaa
    11761 atgaaaagga gtaatgcaaa aaaacttgcg tatacgcaaa ttacaacaga aaaatttgcg
    11821 tattttcccg ttttttgaaa gaaaaataaa
//
LOCUS       DAZUMM010000080        11747 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000080 DAZUMM010000000
VERSION     DAZUMM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.082"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..337
                     /locus_tag="ACG8E6_002167"
     CDS             <1..337
                     /locus_tag="ACG8E6_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5062554.1"
                     /translation="GIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQ
                     YTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYS
                     SRKYINFDK"
     gene            complement(491..1021)
                     /locus_tag="ACG8E6_002168"
                     /pseudo
     CDS             complement(491..1021)
                     /locus_tag="ACG8E6_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            1093..2385
                     /locus_tag="ACG8E6_002169"
     CDS             1093..2385
                     /locus_tag="ACG8E6_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /protein_id="HFS5062555.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSECRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            complement(2597..3439)
                     /locus_tag="ACG8E6_002170"
     CDS             complement(2597..3439)
                     /locus_tag="ACG8E6_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5062556.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
     gene            complement(3476..4942)
                     /locus_tag="ACG8E6_002171"
     CDS             complement(3476..4942)
                     /locus_tag="ACG8E6_002171"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5062557.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            complement(4998..6842)
                     /locus_tag="ACG8E6_002172"
     CDS             complement(4998..6842)
                     /locus_tag="ACG8E6_002172"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5062558.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            7444..8514
                     /locus_tag="ACG8E6_002173"
     CDS             7444..8514
                     /locus_tag="ACG8E6_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5062559.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKNKLSKILSLAKAKTLARLLMFLLKSLVLHKYSQNILNYYFLEKSKKN
                     RIPNFHLHFQKDLHVDNQFLNQ"
     gene            complement(8480..8566)
                     /locus_tag="ACG8E6_002174"
                     /pseudo
     CDS             complement(8480..8566)
                     /locus_tag="ACG8E6_002174"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(8610..9071)
                     /locus_tag="ACG8E6_002175"
     CDS             complement(8610..9071)
                     /locus_tag="ACG8E6_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermophilic serine proteinase"
                     /protein_id="HFS5062560.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNR"
     gene            complement(9331..9879)
                     /locus_tag="ACG8E6_002176"
     CDS             complement(9331..9879)
                     /locus_tag="ACG8E6_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062561.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(9880..10737)
                     /locus_tag="ACG8E6_002177"
     CDS             complement(9880..10737)
                     /locus_tag="ACG8E6_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS5062562.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(11023..11310)
                     /locus_tag="ACG8E6_002178"
     CDS             complement(11023..11310)
                     /locus_tag="ACG8E6_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HFS5062563.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKTLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(11300..11629)
                     /locus_tag="ACG8E6_002179"
     CDS             complement(11300..11629)
                     /locus_tag="ACG8E6_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5062564.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT         3599 a         2355 c         1856 g         3937 t
ORIGIN      
        1 aggaattttt aagttcacag atattaactt agcgcgtgag gctaaaaatc gattgattca
       61 tgattatatc gatcaaccaa aatattcaaa agcttgcgca tcattggatg atggattcga
      121 agacgccttt caatataccg tacaaggaaa ttcccacaat cgactaaaga gtaccaatct
      181 aattgaacga ctgaatcaag aagtacgcag aagagaaaag attattcgca tcttccccaa
      241 tcaaacatca gccaatcgct taattggagc cgttcttatg gacctgcatg atgaatggat
      301 ttattcttca agaaaataca tcaattttga taagtagaaa tggtaaaaac attgtatagc
      361 attttacaca ggagtctgga cttgactaac atttccattt catttacctc tttttcgttt
      421 aattatacta aagttctatc caagcgacaa gctgtgtata ataaaaatat acccgtggtg
      481 ctagttaatt ttaataccct aaagaatatc tcaaagtccc ttcaaaatgt tgctgtgctc
      541 ggcttaaaag aatactgtac gataataata tgctttcaaa cttactttct agcccttgaa
      601 tagaacgtga tttgaaattt tgaaaaccta gtctttcgag agaagaaaaa acagtttcta
      661 ctgcttttct ttgtctagta agtaatcgat ggtctatctt ccctagttta tccatgtttt
      721 ttcgtgacgg aactaagact gtaatgccct taagggcaag cttttcatgg attttatggc
      781 tgacatagcc tttatctcca aggatatttg gaaggtttgc ttcttccgat aaagtctcta
      841 aaactttcac atcatgaaca ctaggatccg tgattgaata agtaactgga aagcctgttt
      901 tagtgacgat aatatgaatt ttaagaccat aaaaataagc gtttttcgtt gagttatagc
      961 ccctacagct ctatactttt tgatgttggt ggatgacacc agcatctttt ttattttcca
     1021 aaacatatag aaaagacacc ttgaaatcct ttagaattaa tttggcgaac caacccaaaa
     1081 ggaggaatca agatgtctta cactcacctt atcaaagaaa cattagatat tctagactta
     1141 tcagttactt ttaacgaaaa ttgtctcaca aaagaaaaat acaaagggca aatctgtcat
     1201 atttatcgtg gaaacttgat ttatacagcc caagaatgca tccactgtaa acaccagatt
     1261 gcttcagata ttgtgcgttg gggtacaaca accgtccggt tgttaatgaa cgatgtttct
     1321 gaatgccgta cctacctcga attgaaaaag caacgcttca aatgcaaagc ttgtcagcgg
     1381 acctttgtcg cagatacttc tgttgcgaaa aaacactgtt tcatcagtga gaaagtccgc
     1441 tggtctgtcg tgactcgtct gaaaaaaaat acctcaatga cagagatcgc cgctcagaaa
     1501 aatctatcgg tctcttctgt ttaccgtatt atgaagcgat tttatcgtcc gttgaatcct
     1561 tttcggacac cgttacctaa ggtactttgt tttgatgaat tcaaatcagt tcggggtgtt
     1621 tcaggtgcta tgagtttcat catgatggat ggacaaacac agcgcttgtt ggacattgtc
     1681 gagaaccgac aactgccttt tttaaaacgt tattttagtc atttttcacg agaaattcgt
     1741 gaagcggtcg aatggatcgt gattgatatg tacgctcctt atgtatcgct cgttaaaaag
     1801 ctgtttccaa aagcacagct gattattgac cggtttcaca tcgtccaaca tattggtcgt
     1861 acctttcgca atcatcgaat caaagaaacg aaccaactat taaaaagcaa agaacaaaaa
     1921 cactatcaat taggtaagca attaaaaaga tactggaaac ttcttcaaaa agatgaacgc
     1981 aagcttgatt atactcgacg cctttggcga cctggcttca aagctcattt aacagagacc
     2041 gatattgtgg atcgcttact gaaagggagc cctgctttgc gtgtaggcta tcagttatat
     2101 caagactttc tttacgctgt aaaggaacga gactacgtat cgtttgaaga actacttacc
     2161 aataatatca tgcttcccga aggctatcag acgaccttga gaacgttcca gaaatttctg
     2221 ccacaaatca aaaatgcttt acaacaaagt tattcaaatg gcccacttga atgtctgaac
     2281 aaccatatca aagtgttaaa acgaaatgca tatggctttc gtagctttta taatttcaaa
     2341 ttgcgcatca tgattcgtca tggcaacgca ttgattttca actaaaaaag atcaagagga
     2401 actgatccac ttgatctcca attattctaa gatttctctt taccaacatc atttgacaaa
     2461 gagccgagat tttttattat cttacgttaa tcctttttaa atgtctctta tagataacaa
     2521 acagctggaa atcaccttgt ttttactata atccatctct aaagatttta aaagcatcaa
     2581 tagtattact gagcgctcac ccattagcat gtttcatcaa ccgattgatg tggacaccta
     2641 ggtatacaac ttcatcgttc ggaattgttt tatgataaac tttttcaatg tagtctttga
     2701 tcttagctgc gctttccatg gcagacgggt ataagatcca catttgattg tagaattctt
     2761 cctcaccatc atccaacaaa gcatcagaat aatatctttc tacaaagaaa cgtacatgtg
     2821 taatgaagcg ggcataatgg atcgaattag tatcgatatc tttctgcaac gaataccgga
     2881 ccaaattgac gatcccgccg atcatttttg catacttgaa tccagcgccg ctatcaggat
     2941 cttccgattg tgcattgatc aaatggaaag caatgtttga ggcttcctct ttaggaagat
     3001 caattccata ttgctcgttc aaaagggtga gtgcgtactt cccaatttga aattctttgg
     3061 gatagtagat ttttatttcc cagtacaaac ggtttgcaat atttacccct ttttgtgcgc
     3121 gttcaactgc aaaatgcagg tggtctgtta aggttaaata gactccagga ttcagttttt
     3181 tatctaatcc tttttctgct tcaaaaacga tagtacgagt gatttcaaaa aattcaatag
     3241 gtatttcttc aactaactct aatagttgtg aagatttctt ttcttcaaaa ggcagaaata
     3301 ttttatctac tttatctcct gatatttcat ccccagcttt ctgtccaaag cctatccctt
     3361 tacctaatgc aatcatttct ttgccatttt cttcaacaag taccacactt gaattcaata
     3421 ctttttttat tgaaatcact cattatcccc ctatcgctgc ttaaaaagat cgagactact
     3481 cgaataagga tgcaccgttt gtttcgatca cttctttgta ccagttgaac gaatcctttt
     3541 tcaagcggtt atatgtccca tttccttcat cgtctgcatc cacataaata aatccatatc
     3601 gtttagacat ttgagaagtt cctgcactaa ctaaatcaat tggtccccag ctagtataag
     3661 ctatcaaatc tacaccttca tcgattgctt ctttcatttg ttggaagtgt gctttaaagt
     3721 agtcgatgcg gtacggatca tggattttac catctactac ctgatcgact gctcccatac
     3781 cattttccac aatcatcaac ggtacttggt aacgatcata aagttcgatc aacgaaattt
     3841 tcaaaccttt tggatcgatt tgccagcccc aatctgttga aggcaaatga ggattcttaa
     3901 cccctaaaac agtatttccc ggtgtgcgtt ctgcgtttgg atcagctgat tctgccatgg
     3961 acatgtaata gctgaaagaa acaaaatcta cggtatgttt ctttaaaatt tctcgatctt
     4021 catcttccat tttaatctga attccccgat tttccaaatc cttttcgatt agagcaggat
     4081 attctccctt aacttgaaca tctgcataaa aattattttc taagttcttt ttcaaggtca
     4141 gctcaacgtc ttctggtgcg caagttaatg gataagtcat caatttcgtt aacatacaac
     4201 caacttggct tcctggaatt ttttcatgag catcttttgt cactaacgca ctcgcaacaa
     4261 attgatgatg caatgcttga tagatatctt gagcttcttg accttctgcg cttttctctt
     4321 ggataattcc agctgttgta aatggatgac ggtggatgct gtctatctca ttaaatgtca
     4381 gccaatattt tacgtatttg ccgaagcgat caaaacaaac gttagcgaag cgaacaaaat
     4441 catcaattac tcgacgctcc acccagccat tatatttaac tgctaaagct aatggcattt
     4501 cataatgaga tagtgtcaca atcggctcaa ttcctaaacg ttgcatttct ttaaagacgt
     4561 tttcataaaa tgcaacccct ttttcattcg gttccagctc ttctcctgta gggaacaagc
     4621 gagtccaagc aatagagagg cgcaaggttt taaagcccat ttcggcaaat aaagctaaat
     4681 cttctttata gtgatgataa aaatcaacgc cccttctttt tgggtaccat ttatcagatt
     4741 tgtcttcact agcttctttt atagactgca gggtgacatg catatgagca gcaaaatctt
     4801 tgttgtctaa atttggttta taagtagcca tatccgctac tgaaagacct ttaccgtcta
     4861 cattccaagc accttctact tgatttgctg ctatcgcgcc gccccataaa aatccttttg
     4921 gaaaacttgt tgtgtttgtc attattctat tctcctctat tccttcattc aatatatttt
     4981 ttttcactat cttgtgttta agctatcgga ctgatagcta agagatcact atcattggtt
     5041 accaattttt ctaggttaaa tgaacggata gtgtaattat ccttattggt taccacacag
     5101 ataactgtgg ggtcgaatcc tgcttctttg attaaatcaa tatcaaatgc aatcagcttt
     5161 tgtcctttgg ctactttttc accagaacga acaaatgcct caaacccttt tccttctaac
     5221 tgaacagtat caataccaat atgaaaaaga agttctgcac cattagccgt ttttaaacca
     5281 atagcatgct tcgtttcaaa taccattgtg atttctccat cttcaggagc tactaatatc
     5341 ccttcttcag gaatgattgc tactccatca cctaccatac cactagaaaa aacagtgtct
     5401 tttacttctg atagtgagat tacttttcca ttcacgggac ttgaaagaac agcatctgaa
     5461 acaacggccg tttccatctt agtttcaact tcattgacaa cttctttatc tttataaaga
     5521 accaacgctg cagcaaaagc aaccacaaat gaaatccccg atactgcaaa tgcccaaacc
     5581 agattcattg ggtcagcttt gtcagcgaac atcgtgatac ttgcaatccc aggacccact
     5641 aaagcaaagg cttgaagtgc aacgattcct gcaaatgctc ccccgatcag gctagaaaac
     5701 ataacggcat ataaaacacg tttgtgcaga atcgttaccc cgtaaagagc tggttcagta
     5761 attccgaata atgcagatat acccgcagaa acagccgtag atttcaattt tttgtctttt
     5821 gttttgatac tgacagcaaa gcatgcgcca gcttctgaaa tattgtgtgc caatgaagcc
     5881 gggagataaa gaagttctgc acccaattca ctcatggatg aaacagcgta agggatcatt
     5941 gctttatgca tacccgttac aaccattaat ggcagtacag ctgctaagac tccggttgca
     6001 acccatccaa attgattgta caaagacaca atgatagaag agaaagctgt tcctacatta
     6061 taacctaatg gtcctagaag caacaaagca ataggaactg taatcaataa gctcatcata
     6121 ggtacaaaga aaatacgaat tggttttggt gaaacttttg taaaccattt ttcaagttga
     6181 gcataaaata aaacagttaa gatggctggg aatacttgag atgcatacgc tatattatcc
     6241 aatccaaaag ataagaatgt agcaccttcc gccaacatag ctgtcatttc aggaaggatc
     6301 aatgcaccga cagcagatac tgcaaccaat tgattgactt tcaatttatt agccgtagtc
     6361 attgccacca gaataggcaa gaaataaagc ggtgccccgc cgaccaaatt taaaatctga
     6421 taggtttgag attggtcact aatcagccct aagatagaag ctagtagcaa caaggacttc
     6481 aatactcctc cacctgcaat cgcaggaatt aaaggttgga aaatagaaat aataaaatct
     6541 aatagaactg aacctaattt ttgtttttgt tttggtgctt cttcagtatt tgaaacattg
     6601 tcacctaata acgctttcac ttcatcatac acttcaacaa catcgtttcc aatgattact
     6661 tggcattgca cattttggcg aacgcccata acgccatcca ctttttctaa ctggctcata
     6721 tttaccttgc tgttgtcttt taacgtaaaa cgtaaacgag ttgagcaatg ctccaaatgg
     6781 gatatgtttt cttccccacc gatgtactgc aatacttctt ctgctgtttg tttgtaattc
     6841 atggtgtttc cctcttttct tttttgagtg atgaactttt gagtcgaagt ggccatcctc
     6901 aacacaaaaa acaggcagaa actaaataaa ttgacaacaa ggcaaacgtg tattgaaacg
     6961 catacattgt tattcaactt atttaggtcc tgcctgcatt accagtcaca tgcctgttta
     7021 agttatattc aatttaacat attagaatgt aaacgtaaat aataaaatga ttttttaatc
     7081 attttttcat tttattcgtt caatatagtt ttcgtgttct aaaagttttg tcttttttct
     7141 aagaatatct tccaacatat ataactctta ttctcttcta tcttctagca gatgttctgt
     7201 ctttttaaga cctagtataa aaagaatggg ttcatttttt ctttcttgca ggatgaggtt
     7261 ttttatataa actttatttg atataaagaa aaggcattca taattgtgct atactactaa
     7321 aaaatgataa tacagtgaat tagattagcg ctttattaaa ttaagaggac agcatatcga
     7381 tattgcttcc ttttttttgc tatttattta agggatagaa gtttagaaag gactgtttat
     7441 aagatgaaaa atgatatagt gacttatgca aacacaaaat gtgaagaaat tggcatcagt
     7501 aatttagaag agtgcttttt tgacacaaat gacctgtcgc aatttttaag aacgggaaga
     7561 acaaatgcca gtaaacttgc caatgactta gttgcaaaga acgttctatt taaaatcaat
     7621 actcgtccag tgttatttgc catcaaagag cgctttgaag agcacttttt gattccggta
     7681 aaagatgtat acgaaagtat atctgaatta aaaacagact tggagcccaa ttctacagat
     7741 tccattctac aaaagatgat tgggtatgat atgagcttat ccgatgcggt ggaacaaatg
     7801 aaaactgctg tcttctatcc tggaagaggg cttcctatga tgatcactgg agatactgga
     7861 gttggaaaaa gtttttttgt tcatactgct tatcagttta tgaaaaaagt gaacgttatt
     7921 catgagaatg ctccttttaa agtacttaat tgtgcgcaat accataataa tcctgaatta
     7981 ttgtcgggga tactatttgg ctatgttaaa ggtgctttta ccggcgcaga tgaagatcga
     8041 aatggattgt tgcaagatgc agacggtgga gtactgtttt tagacgaagt acacagattg
     8101 cctcctgaag ggcaagagaa actatttgtt tatatggatg gcggaactta ttctagagtc
     8161 ggtgaaacta tccatcacgc ctccaatgta cgtttagttt ttgcgactac agaaggcaaa
     8221 gaggattttc tggaaacctt tatgcgaaga atccctatac atatccatat cccaaatatt
     8281 gaggaacgag gtattttaga aaaaaacaaa ttatcgaaaa tactctcttt agcgaaagca
     8341 aaaactttag caaggctatt gatgtttctc ctaaagtcac tagtgttgca taaatatagt
     8401 cagaatattc tgaattatta ttttcttgaa aaaagtaaaa aaaatcgtat cccaaacttt
     8461 catctacatt tccagaaaga tcttcatgtt gataatcaat tcctgaatca atgaccgcta
     8521 ccttcacatc cttagaaccc ttggtttctt cccaagcgga tggcatgcta atctctctta
     8581 aattagcttg tttactccaa ttaggatcgt tatcgatttc ctaaagaatc agttgtattt
     8641 ggctcctcaa catcattcaa cggctctagt tcaaccacat agtttggctc tgcataattg
     8701 accatatcag aagcattaaa tgttcttact gcctcttcca cactaatttc ttcgttcaat
     8761 ttcactaata atacttgatc tcgcttatta taatcgcttt catttgtcct aagagtcaaa
     8821 tctttttcca ccataacttt tacacccaat aaattttgcg gtttattttt ctctgttgaa
     8881 attctttgtg tagcctttaa actgacaatt agctcattgg gtacatactc tatttgagag
     8941 tcatctaatt ggctcataat cctttctttt tggttagtat ctgcagttac ttgtttagta
     9001 tgagccatga acccaaacat actcacccca acaaccaatc ctaaatatat tttcttttta
     9061 aactttttca tctacatctc ctcctaatag ttttatttta gagttagctc tattacttat
     9121 ttactaactc aacaacagat tatcatacac ttattaatga aatcacaata tctcattaaa
     9181 acacacccta aaatcataac cgttcctatg ctcatttttt ggatttttta atcacgtaac
     9241 cagaaaaata aatcccctct atttcacgca aatcaaagaa gaactaagta actaccgttt
     9301 aaaggtagtt acttagttct tctttgatta ttatctaact ttattattca ttccccaccc
     9361 tttgcgcagt gagtcttcgg tatcttgttt tatctgactg gtagttctta ttatttctcc
     9421 acgatcatct aattctggaa caattggata tttttctctg aacaagtcta gttctttcac
     9481 cacgtcatta aacaaatctt gtttatcttc ttgcgtaaga ttctgtttaa tatactcatc
     9541 taaagctttt ctaagcaatt catctcttgt catattagtc tcatactttt ctactaaaac
     9601 tctggaagtc gcatttaaca aagtcgacag ttttaaagga tgcttggtgg aataaacctt
     9661 atcaaacttt ctgattggtg cgccaacttt ttttgttctt cctctactct gtctttcaac
     9721 attggaggaa gtttttattt cttgaacttg gtgcacttct aattgcttat tttcagatgg
     9781 tgtaattgct tgctgctttt tctttgcttc tgcttctttt tttattttgg cgagtccttt
     9841 gttgttccca aaaatatcaa gtcctttgat gtcagccaat tactccacct ccgcatacca
     9901 attctcgtgt tcttccaatt ctttcaaaat actgataaat aattcgtggc ttttgcgatc
     9961 ccattgtttt aagagacctt tctttgtctt ctccatcgta atacctgatc cgtcgtatcg
    10021 ttttaagcgc gcatttttga gtacaacatt tttaatcaaa tgttccccat aaatctcttg
    10081 ggcttgttta tacatttcct gatctactgc gtcaccgctt tccaacatga aaggaataac
    10141 accagcaaca tataaatttg tatcaaattc tttcacaaaa aagtccatat actcaagata
    10201 ttgcatcgca tttctcatcg attttacttg ggtctgtaag acgacgattg cccaatccgc
    10261 aatgtacatc gcggatttac tatatacaga aatagaaggt gggacatcta aatagatgac
    10321 gtcaaactca tcaaataatg gttctagctt ttctttcata tatttgaatt gggataatga
    10381 atcattatat tggtctttga tccaatcatc atacatttca aaggacatgt ctgctggaag
    10441 tatatagagg ttattgatta ctttatagac agcctcttta ggtggaagat tgtttatcaa
    10501 cacgtcagtc acagtatttt cgtaaacaat cggttcacct tctccgcctc cttcttcgtt
    10561 agtgaagttc atatgcgtta agttcatcac atcagaagta tttccttgca tatccaagtc
    10621 aattatcaag gttctttttc cttcattaac tgctgattca aaccctaaca tttgcgcgca
    10681 cgtactttta cctacgccac ctttaaaatt accgattaca tattttttcg ctaccacgtt
    10741 cctacctctt ttcttttagc aatattcatt tgatactatt ataacatatt tatttttagt
    10801 tactaccaaa aaaaagatat atatgtacta ccttataatt aataagtaaa aggtagtaca
    10861 taaataagga aaagctgaaa tatattgatt tatagctttc ctcaaagtaa tgacgttatt
    10921 taatttatcg agaagcagag aatactcgcg actttagtca tgtagatgaa tcagatcttc
    10981 gataaaaaag aagccgtcct tcttaagaac gacaacaaat cactagatta attttttcaa
    11041 ctgttcataa aagttttcat gcgttccaca taacaatact ggaataacct tgttttctat
    11101 aatctcgtaa gctactctat atgttgtgcc cgcatatttg aacccactag accaaatacc
    11161 tgataaatcc ccagtttttt gttcaccctt atatggatca atcgcaattt catcataaat
    11221 taaattcaaa aatttttctt tcaatgtctt ctcttttagc ttttttaaat acttaatgac
    11281 atgttttgaa ggaacgattt cataagccaa tttctgttgc caactcctct ttagtcatta
    11341 ctttagaatt agtaagtgta tcttcagcaa tatctctaaa ggaagaatgc attttgtttt
    11401 ttctattctt aaattctaca agaatctcat ctttatcgta gccttctgca attatatcag
    11461 acaaaatatc ttcgctaaaa tcataaaatt ctttttgtgg ttcaacaaat tcataaagaa
    11521 taccattctc taccaacttt gcttcaactt cgactccatt aggaacatta aaatctttag
    11581 gtactgtcaa catgattgaa ttgccttgtt ttcttgtttt tgtaatcatt taaatccctc
    11641 atttctaaca gtatgtaatt acttagtaaa tacattatac cacgagataa tgtatttaca
    11701 agtaattact gtgactcgta atgcgtcctt gctgaataaa tttcaac
//
LOCUS       DAZUMM010000081        11249 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000081 DAZUMM010000000
VERSION     DAZUMM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.083"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACG8E6_002180"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACG8E6_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFS5062565.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACG8E6_002181"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACG8E6_002181"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFS5062566.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="ACG8E6_002182"
     CDS             complement(2169..2906)
                     /locus_tag="ACG8E6_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS5062567.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="ACG8E6_002183"
     CDS             complement(2997..3524)
                     /locus_tag="ACG8E6_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062568.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="ACG8E6_002184"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="ACG8E6_002184"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFS5062569.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="ACG8E6_002185"
     CDS             complement(4401..4886)
                     /locus_tag="ACG8E6_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFS5062570.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="ACG8E6_002186"
     CDS             complement(5039..5236)
                     /locus_tag="ACG8E6_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062571.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACG8E6_002187"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACG8E6_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFS5062572.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACG8E6_002188"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACG8E6_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFS5062573.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="ACG8E6_002189"
     CDS             9211..9423
                     /locus_tag="ACG8E6_002189"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFS5062574.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="ACG8E6_002190"
     CDS             9425..11104
                     /locus_tag="ACG8E6_002190"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS5062575.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3261 a         2429 c         1971 g         3588 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaa
//
LOCUS       DAZUMM010000082        11229 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000082 DAZUMM010000000
VERSION     DAZUMM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.084"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..304
                     /locus_tag="ACG8E6_002191"
     CDS             5..304
                     /locus_tag="ACG8E6_002191"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5062576.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            377..1738
                     /locus_tag="ACG8E6_002192"
     CDS             377..1738
                     /locus_tag="ACG8E6_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS5062577.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1760..2020
                     /locus_tag="ACG8E6_002193"
     CDS             1760..2020
                     /locus_tag="ACG8E6_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062578.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2034..2786
                     /locus_tag="ACG8E6_002194"
     CDS             2034..2786
                     /locus_tag="ACG8E6_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFS5062579.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2880..3485)
                     /locus_tag="ACG8E6_002195"
     CDS             complement(2880..3485)
                     /locus_tag="ACG8E6_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5062580.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3538..4512)
                     /locus_tag="ACG8E6_002196"
     CDS             complement(3538..4512)
                     /locus_tag="ACG8E6_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFS5062581.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4890..5609)
                     /locus_tag="ACG8E6_002197"
     CDS             complement(4890..5609)
                     /locus_tag="ACG8E6_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5062582.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5736..7007)
                     /gene="celB"
                     /locus_tag="ACG8E6_002198"
     CDS             complement(5736..7007)
                     /gene="celB"
                     /locus_tag="ACG8E6_002198"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS5062583.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7160..8557
                     /locus_tag="ACG8E6_002199"
     CDS             7160..8557
                     /locus_tag="ACG8E6_002199"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5062584.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8638..9078
                     /locus_tag="ACG8E6_002200"
     CDS             8638..9078
                     /locus_tag="ACG8E6_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5062585.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9226..9552
                     /locus_tag="ACG8E6_002201"
     CDS             9226..9552
                     /locus_tag="ACG8E6_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5062586.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9553..10302
                     /locus_tag="ACG8E6_002202"
     CDS             9553..10302
                     /locus_tag="ACG8E6_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFS5062587.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10382..10837)
                     /locus_tag="ACG8E6_002203"
     CDS             complement(10382..10837)
                     /locus_tag="ACG8E6_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5062588.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3561 a         1961 c         2302 g         3405 t
ORIGIN      
        1 aaaaatgaaa aatcggttaa ttgtcgcatg tggaagtgga gtagcaacga gtcagacaat
       61 cgctagtaag atccaaagta tgttagaaga tgacggcatc gcatttcctg tagaagcagt
      121 tgattacaaa tcgattcaaa atgagcttct aactgctgga atctatgtgt atgtcgcaca
      181 acctgatgag gaagtacttg aacaagcaaa agatttgggt attgaagtat ttccaggaat
      241 tccattttta actggaatgg gtgtagaacc tatttatgac agtatcaaag agttagtaca
      301 gtaaaggagg attgttggat aaaaaagttg gttcatagtt ttatttacta tatttagaaa
      361 gaaagaaggt ttatttatgg aagttttcca aaccattatc aattatgttt tgaacttagg
      421 atcagccatt tttgttcccc tgattatttt gatacttgga ttgttggcag ggatgaaatt
      481 aaaaaaagca ttcatgtctg ctttgacttt agggattgct tttagcggaa tgtctatggt
      541 cattggctat atgtctaacg cagtaagtcc agcatcagaa gcattggcta aaaatacggg
      601 aatcagtttg cctgcacttg atcttgggtg gacaggtgca gcaagcatca catggtcgtg
      661 gagttatgct tttatatttt ttgcagtaac gattggggtc aattttgtac tgttactttt
      721 taattggacg aagacgttaa atgttgatat gtggaatgtt tgggggaaag ctttaactgc
      781 ttatttagtt tattatgttt ccggtagttt ggctgcaggc tttcttactg caatggtcca
      841 agttatattg gaattaaagc ttggtgatat gtttcaaaaa catatacaag atctcacagg
      901 gattccatta gtaacagtga ctcattttat gacatctgcc gctgtcttgt tgctgccttt
      961 caatatgatc atggataaaa ttcctgcttt aaataaacga gcggatacga atgcattgaa
     1021 gaagaaaatt gggatcttct ctgaaaactc tgtaatggga ttcattattg gattggggct
     1081 tggctttgct gctgcgtatg gcgtttcagg ttctcttaat ctagcgatcc aaacagctac
     1141 agcattaagt ttgtttccta tgatctctaa aatgttcatg caatctttgt ctcctctagc
     1201 agacgctatg tcagaattta tgaaaaagcg gtttaaggat cgtgaagtat atatcggatt
     1261 ggactggccg atattagctg gtcgttcaga aatttgggtt actgctattt tacttgttcc
     1321 tgtgtttatt ggttatgcca ttattttgcc aggaaaccaa gtattgccat tagcagggat
     1381 tatcaactat tcgattgcgg tcggcggatt acttttaaca ggaggaaact tggctcgtat
     1441 gctgatcttg ggaatcatca ctatgcctat ttatttgtac ggggccactt accttgcgcc
     1501 aatactcagt gatttagcag aaaaaagcgg agcagtcgag ggaatcaaga aaggacagtt
     1561 gatcacatgg tcatccatag aagggccgga gttcagggtt ttatttgcgg aggcatttaa
     1621 taggaatatt ctcgctattg taggatgcgt gatttttcta ggactgtttg ttctacttta
     1681 taaatatatg ataaaagcga aagtaccgag tgaacgttat gctgaactac aaaaatagca
     1741 ttgaaatggt gggaaagtaa tgaaaagaga agagacagat aaaataaaat ggacagtcgc
     1801 tctttgtggt acacttctgc tatttctata tggactcttt actcagaata tcatcatcaa
     1861 tctattggtc atttttttcg cactagtgat ctataaatat ggaaatcacg ttttattcag
     1921 agagtacgat gaaaaaagaa aacggaaaat agaagaatca atgaaaataa aagaagctac
     1981 caaagaaata ttgagagaaa aaagttttat aaaaagataa tggaggggtc attatgtcag
     2041 atggacgtat gttatttgag agagaacgac aggacatggc acgtatcgta agagtgattt
     2101 tcgaaaggaa aaacacaaat gtcgcaggag ggaatttttc ctttaaaacc actgatgatc
     2161 aagggaaaga ctacatcatc atgacgccga ccatgatgtc agaagcctat ctaggagaga
     2221 tttctcctgc acaggttcta gtagtagaac caagaacgcg tagaatcgtt gacggagtcg
     2281 gtcatttaac tcgtgaaatc aatatgcatg aagcgatgta tgagactaat ccagctatca
     2341 aagctgtcct acatgctcat gcgcctaatg caatgttctg ggcgactact gggttgccta
     2401 tgcctaattt gacagaagcc acagctaaag taaaagagat tcctgtcatg gactttgagc
     2461 ctaattgctc tgaagaactt gctgaattga caagcgacta tgtaaaaaaa cagcatctcg
     2521 aattaccgca tatgttgctg ctcaactctc atggcgtatt gatcaacaca ggtggagaat
     2581 caggaatcga agctatccat aaagcactgc aaatccttga tacagttgaa tggaatgcag
     2641 aaattgccta caagcagacg atttttcaag cattagggat tactgacggt ttttattcaa
     2701 aaggtgagaa aatcggggga ttggaagagt tgaaacataa aacggctatt tataataaat
     2761 ctgttattgc agctggtgga gactaaccta tctgacacaa aaaactacga ctttcacaaa
     2821 atctgatcca gatcgatatc tgcttcaggt tgtattatgt aagtcgtagt tcttttcttt
     2881 tatttagtta agaggtggtc tgtccattcc ctcgcttgct tgatttcgtc catcgtcaac
     2941 tgatgacctg ttacagttag ctgaatagtc acatttgctt ggcgtgtttc gaattgatcg
     3001 gccaattctt taaaggaagc aggggaaacg attggatcgt tatcaccata agaaatccag
     3061 acggttttat cctctaatgt ggaacggctt tgatgaacac ctagagacat cggatggaaa
     3121 aacaaccctt tatttagctc ggtctcacgc tccaataata aatgagcagc gatgtttgcg
     3181 ccatttgaat agccgacaag cagccagtca cttaacggta tcagttcttt ttcgctgatt
     3241 tctttgattt tttcatggag tttatctgtt tctttttcca aactgtccag gtcgaattga
     3301 ttcaatccat tacgcttgaa aaaacgattc atcccatctt cttggaccgt gccacgaaaa
     3361 gaaagcaatg tactgtttgg tgcaagaaag tgagcgatat ccagtaaaga atgttcatcg
     3421 ccacctgtgc catgaagtaa gatcaaatgc ttgccacttg ttcctttttc aaaaatgtag
     3481 gtcattttgt tccctctttt ctgtttctat agatgcaaat gaagtagcat gaagagatta
     3541 ttcacgtttt ttatttgcat cacttgtatc gaaaggacgg acataagctt caatggcttc
     3601 acgacgttct tctaagaatg gaggaagaga tagtttttct cctgctgatt ctgctggttc
     3661 atcttcaagg aatccaggac catcagttgc taattcaaac agtacgtgat gagcggctag
     3721 gaagtactca gattggaaat agaaacgatc gacgaaacca gaatttggca attcaatctt
     3781 attgattact tgaatccagt attccaatgc ttctttgtct gctacacgca atgctaagtg
     3841 atggatattt ccaaatcctt cgattgcttt tggcaagtct gtccgatgtt caataatgat
     3901 gcttgcacca tttccccctt caccgatttc aaataagtag aaatctcctg aatggtctgt
     3961 ttctttgaat cctaagattt tttcaagaat caagcggata tgctctaatt gactgacacg
     4021 gacaaagaca ggtccaagtc ctgtgatcgc aaactctgtc ggtacattgc tgtttttcca
     4081 aggaatgcca gctgctacac cttgattgtt ttcgtcagag atcaattgat aatactgatt
     4141 atcgaaatct tcaaattcga tatatttttt gccgaaacgt tcattgattg ctgaatgagt
     4201 aacgtcatat ttttcaaaac gttccaacca aaagtctaat gctgcgtcac ttggcacacg
     4261 gaaagaagca cgagagatcg tatctgttcc tttttttcct tttggctgat ttggaaaatc
     4321 aaagaaagtc atatctgttc ctggcgcacc agtatcgtct gtaaagtaaa gatggtaagt
     4381 ttcgatgtca tcttggttaa ctgttttttt gattaagcgc attcctaaaa tatctgtaaa
     4441 aaagcggtag atttttgtag cgctagatgt cattgctgtg acgtggtgaa ttcctctgat
     4501 ttgtgtatcc atgataaatt tcctcctgtt aacttacaaa aagtaagtag ttgatttaga
     4561 catagtatag agggtgcatc tctattttgc aattaatttg tcttctaaag tatcaggaag
     4621 gaatcaagaa tggaaaagag gagggacgga tcaataaaat aaaaagactt acgactagct
     4681 gtatcgattt caaaaaaact agctttaaaa agctaacttt agaatcatta cgggtggtca
     4741 taagtctttt tatgctgagt tattttttca tttttgagct aatgcttagg atagaataga
     4801 atgttgattt cacgatctct tcttgttact tgaaactgct taccactgtc acgatctctt
     4861 ggtaaacggc tttttcacaa cctctttggt taagttgaac gcacatccag cacagaatag
     4921 ttccgaacat caaatcggtt tcggctggaa gaatattcaa tcggctgtcc atctttgaga
     4981 tagacgactt gctgaacttc cagtacagga tcgttttctg cacagttcaa atattgctga
     5041 tcatattcat cacttttatc tgcagaaata gcacgataag cccctgcaaa atggacattc
     5101 aattcttgat gaagatgatc atagatagag gctaacaaat gttctttttt taaacccggc
     5161 acaagatgga ctggcataaa ggtatgctct aaaacatatg gctcgccatc taagatcctt
     5221 aaccggtgaa tattgtagac aggttgctcg gcatcgatca tcaatcgttc ttggatatat
     5281 tgattaggga attcaacatc aaaatgaatg atttgactct tcaaagaacg atgtctgcgt
     5341 ttcatttgga gactcaaacc ttcatactcg ttagcgggcg atgtgtcttt ttgcataaat
     5401 ggatgattca agattttcgt tcctgatcct cgctgcgaat agacaagtcc ttccattgct
     5461 aagatattga tggctttttt gattgtcatc cggctcgcac caaattcttt taccaagtcg
     5521 acttgattag gcaaaaaact atcgagagga tactcttttg aacggatacg ttctcttaaa
     5581 atatttgcga tttcttcgta ttttggcacg ttattcacct cttctagagt ataacgaaag
     5641 attatgcttc cgacaactcg aagtagcatc tggaacattg tttattcgaa aaaagaggtc
     5701 atgacaagtt tttgtcacaa ccccttaatg atctattaag caatatctaa atctggttgt
     5761 ttatctagta ctttcaggaa tgggtaccag ataaaggcaa cgatgacaaa gtcgatgact
     5821 tgcagcatac caccaagcca tgaatttgtt gctaagacac cgcttgcaat gattggcaca
     5881 gtccatggaa cagagacgcc agttggtgca gggacaagtc cagcagccat taccaaataa
     5941 ttgaacgtcg ttacgattag aggtgccaca acccaaggaa taaggattgt tgcattcaat
     6001 acgatcggta gaccgaagat cgctggttca tttacattga aaataccagg tgctaaagct
     6061 aatcgtccaa catcacgata ctgtttcttt ttaagaacaa aggccatcaa taaaacgaca
     6121 gctaaagtca ttccagagcc accaatacca acagtgaagg tttccatgaa tggttttgtg
     6181 atgatgtgtg gtaattcttt tccatgttga taagcttcta agttatcaag catcagtgta
     6241 ttccaaatcg ggtccattac agagttgacg atgatttgtc cgtgaagtcc gaagaaccat
     6301 aagaattgga caaagaacaa agcaatcaat gttgcaggta aactacttcc caaaccagtt
     6361 aatggttttt ggatcacttc ataaacgacg tcatgtaagt tagcattgaa aataccagtc
     6421 atcacagcat tgattactaa gaaaatagtt agtgtcacta cagcagggat caaagcggca
     6481 aatgattttg ttactgcagg cggaactcca tcaggcattt tgatctgcca gccgcgtttc
     6541 gtgattctgc agtagatttc tccagccaag aaagcagcga tcattccaat gaacattcct
     6601 tttgcgccaa gacgatcaag tgaaagcaca ccagtcacat cttcaccgcc agcggttttc
     6661 attgcaaaag gcgtaagaat caagaagctg gcaaaagaca cggcaccacc gaaaatacct
     6721 tcaacgtcat aagattttga taaataatag ccgataccaa aagtaacaaa aaccgacatg
     6781 atgctcattg ttgcattttg cccattaccg aataaattcg ataaagttcc ctttgttgcg
     6841 tcactgaaaa aaggcaagtt attgataacg acaacgatcg aaccgaacat cgttaaaggg
     6901 aaggaaagca tgaaagcatc ccgcagcact gtaaggtaac gattttgccc taataaatta
     6961 gctaagggca tgattttctc agaaagcttg tccataaaac cgttcatttt tattcacctc
     7021 aaatttattt gcaatcgatt acatacaaag tataacgcca aaaaatttaa atgtctagtc
     7081 attttcataa aaagtattga tttatttttt agggttcgtt atgattacgg tatcaagaaa
     7141 aaagagagga agtaaaaaaa tgaaaaccta cacattccca gaaaattttt ggtggggttc
     7201 tgctgctagc ggaccgcaaa cagaaggaag agtaagtgat gatggtaaag gagaaaatat
     7261 ttgggatcat tggtatgaat tagagcctga aaaattcttc gatcaagtag gacctgaaaa
     7321 aacttcgcaa gtttacacaa aatacaaaga agatgttcag ttaatgaaag aaactggcca
     7381 taacacattt cgtacatcta tccaatggag tcatttgatt ccagaaggga caggtgaaat
     7441 caatcaaaaa gctgtttctt tttatcgaga ttattttact gaattgctgg ataatggtat
     7501 tgaacctttt gtgaaccttt accatttcga tatgccacta ccactgcaag aaaaaggcgg
     7561 atggttatca agagaaacgg tcgacgctta tgtttcttat gcagcagcgt gctttgaact
     7621 gtttggagat ctggtcaaaa aatggtttac tcataatgaa ccaatcgtac ctgtagaagg
     7681 aggctacttg tatcaattcc actatccaaa tcatatcaat atgaaagaag cggtccaagt
     7741 aggctatcat gagaatctag caagcgcaaa ggtaatcaaa gcctaccatg aaggcggaca
     7801 tgatggtgag atcggaatca tcttgaatct gacaccaagc tatccgagag atgaaacgaa
     7861 cccagaagat gtaaaagcgg cgaagatcgc tgacgcattc ttcaaccgat cattccttga
     7921 ccctgcagtg aaaggagagt ttccacaaga gttgatcgaa attgtcaaag aattggctat
     7981 aatgccagag atccatgaag gagacctaga actcatccgt gaaaacacga ttgacctgct
     8041 aggaatcaat tactatcagc ctcgtcgaat caaagcaaaa gaaactccaa tcaatcacga
     8101 acatagcccc atgccagatg attattttga taattacgac atgcccaata aaaaaatgaa
     8161 tccatatcgt ggatgggaaa tttacgaaaa aggaatctat gatattttaa cgaatacaag
     8221 agaaaactat ggtaatatca aatgctatat ttctgaaaat gggatggggg tcgaaggaga
     8281 agaacgattt gttaacagcg aaggaattat tgaagatgat taccggatcg agtttgttac
     8341 ggatcatttg aaatacgtcc atcaagcaat ccaagaagga accaattgtg tgggttacca
     8401 catgtggaca tgtatggaca actggtcttg gacaaatgct tataagaatc gctatggctt
     8461 tatcgctgtt gatttggcac aagacggtaa acggacgatc aaaaaatcag gtcgctggtt
     8521 taaaacggtc tctgaaaaaa acggatttga tgcctgatat tttttgataa aacgttcaca
     8581 aaatgtttgt tctaacactg taatcgtgct acactaacag tgaaggaggc gtttacaatg
     8641 gtagatactt atcgtaatat tttagtagca attgatggtt ctgaaaaggc agaaaaagca
     8701 tttcttgagg caatcacgat tgccaaacga aatcaggcaa cgttgcacat cctgtatgtc
     8761 aatgaagtaa caggcaatta ctttggtgat tttgcatttg ttacaacaag ccttcaggaa
     8821 gaattggatg aggttgcgga aaaccagatg aaagaacatc gaaacttggc aattgaaaaa
     8881 ggcctgacag atatcgagac atatgttctt tacggttatc caaagacact gattgctaat
     8941 ttccatgaat cgaaagaacc aatcgatctg attgtcatgg gggcgacagg tctgaatgcg
     9001 gtagaacgtg cactagtagg ttccactact tcctatgtag tcaaccacgc atcatgtaac
     9061 gtcatggttg taaaataaat aaattaaggg aacagaaagc aaaaaagcat aacgcttctt
     9121 ttgctttctg ttccttttat ttttttgtaa aaaattactt tacctgatga agtaatcatg
     9181 ctattctaat tacaacgaca gatggagtaa tatggaggaa agaaaatgat ttcaagtgat
     9241 ggaatacgcg gatacaatga tgcgattatt ttatcgatcc ttaacaaaaa cgattcttat
     9301 ggatatgaaa tttctaaaac gattcgtgag aggacaaatg attcctatgt gatcaaagag
     9361 acaacacttt actccgtttt tgcccgttta gaaaagaatc aactgattgt ttcgtatcct
     9421 ggagaagtga ctcatggaaa aaagcggaca tattataaaa tcactgaaat agggcgtaat
     9481 cttttagaag aaaaaaagca agagtggctg acgacgaaag acgtagtcga tcaatttttg
     9541 gagggaaatt aaatggatac aatccgtaat tatttagaca gtctttttat cggagtacct
     9601 caaagtaccg agatagataa gctgaaaact gatttgttgg caaatatgga agaccattat
     9661 catgaattga tgggagaagg aaaaaatgaa caggaggcca tcggtacagt aatttcgaca
     9721 tttggttcaa tcgatgaatt attagaagaa ctagatgtcg aaaaaaaaca tcaagctgac
     9781 gagacggaaa caaacacagc ttctatttat ttaagcgaag cagaaaacta ctggaaagaa
     9841 tacagagcgg caagtctcca agtggcatca ggtgttctat tcattagctt atcttttgca
     9901 agttttcttt tcttttgttc agcaggatat gtgttcatgg gtatttcttg tctgattttc
     9961 ggtatagcat tagctgtcgg tttttttatt gcttctggta tgaaaatcac acgtttaaat
    10021 cactttttgc accatcggaa aatacctgaa aaagtattag cagaagcaaa agaaaaagaa
    10081 gaagagtatc agcgaagttt tggcttctca ctgattgcag gaattggatt atgtattttt
    10141 tcacttttcc cacttttggc ttctttaatg tggtatatgg atggtagtat cggtgcctct
    10201 atattttttg ttacggtagg aacgggtgtc ttcttgatca tttatggcag tctagtccgt
    10261 cattcttatc gtcaattcac tcaaagcgct tattactggt gaatgtattc agatttgtaa
    10321 aaatattcta tttatccggc gtagaaaaaa agactgtgac acgcttttgt cacagtcttt
    10381 tttatatata ataatttaaa aattcatcat ctaaatccgt agaattttct agattgctta
    10441 cccatgtaaa atcgttatag acagatttca gttgacttct gcgccattgt acagggataa
    10501 aaagacgcat gctatcttca ttgaatccga ctaataattt ctttccgtca gtaatgatgg
    10561 gacgttgtaa caaagtaaaa tgcgagacga tcagatcgac aagctctgag gtcgtacatt
    10621 ggtcgatttg gtctttcaaa tttttataat caatcgagcg tttagctaat agatcgtcaa
    10681 atccgttttc tgttaatgac aataaataaa atatctcttt ccttgttaaa ggctgtttag
    10741 gtgtgaaaat tcgttctctg taaggcattt gatggtcttt cagccagttt gccatcagat
    10801 ggtaactatg tgtgcgagaa gaatagatcg taagcataaa aaaactcctt aaatagtcca
    10861 ttaatttgtt agatctgttg agataaataa taggtaagga gctgtgcata agcattgatc
    10921 aaaacaaccc aaacatcatt agaaataact ttctaaagtc ttaaacttgc gacgagaaac
    10981 aactagacta atagaaaaaa tgtcttagcc atttggcttc ctgtttgttt ttttgcttat
    11041 gctgcagctc ctcgggaaag ggttctaaca atttgaagaa ggttgatgat tgatctagtt
    11101 taaattgtgt gaaatttgaa atctggtgtt tttactagat gaatcaccta acaactatga
    11161 ttataactta tagaacttct gttttgcatt cggttttgtt atagttgtta taaagtttta
    11221 ttttattac
//
LOCUS       DAZUMM010000083        10456 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000083 DAZUMM010000000
VERSION     DAZUMM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.085"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..646
                     /locus_tag="ACG8E6_002204"
     CDS             23..646
                     /locus_tag="ACG8E6_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062589.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /gene="trpX"
                     /locus_tag="ACG8E6_002205"
     CDS             1096..2145
                     /gene="trpX"
                     /locus_tag="ACG8E6_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5062590.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="ACG8E6_002206"
     CDS             2142..3023
                     /locus_tag="ACG8E6_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5062591.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="ACG8E6_002207"
     CDS             3026..3796
                     /locus_tag="ACG8E6_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5062592.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="ACG8E6_002208"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="ACG8E6_002208"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFS5062593.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="ACG8E6_002209"
     CDS             5107..6270
                     /locus_tag="ACG8E6_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5062594.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="ACG8E6_002210"
     CDS             6387..6797
                     /locus_tag="ACG8E6_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5062595.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="ACG8E6_002211"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="ACG8E6_002211"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS5062596.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="ACG8E6_002212"
     CDS             9370..9654
                     /locus_tag="ACG8E6_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062597.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="ACG8E6_002213"
     CDS             9814..10050
                     /locus_tag="ACG8E6_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5062598.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="ACG8E6_002214"
     CDS             10047..10445
                     /locus_tag="ACG8E6_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS5062599.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3643 a         1760 c         2221 g         2832 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaa
//
LOCUS       DAZUMM010000084        10419 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000084 DAZUMM010000000
VERSION     DAZUMM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.086"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1133
                     /locus_tag="ACG8E6_002215"
     CDS             <1..1133
                     /locus_tag="ACG8E6_002215"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFS5062600.1"
                     /translation="SFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRINKVP
                     MPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPKQKS
                     IGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDGLDG
                     LAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLGDTG
                     ALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTADKK
                     HLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAILLFE
                     LIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            1126..1926
                     /locus_tag="ACG8E6_002216"
     CDS             1126..1926
                     /locus_tag="ACG8E6_002216"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5062601.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            1926..2753
                     /locus_tag="ACG8E6_002217"
     CDS             1926..2753
                     /locus_tag="ACG8E6_002217"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5062602.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2758..3465
                     /locus_tag="ACG8E6_002218"
     CDS             2758..3465
                     /locus_tag="ACG8E6_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5062603.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            3565..4431
                     /gene="rfbA"
                     /locus_tag="ACG8E6_002219"
     CDS             3565..4431
                     /gene="rfbA"
                     /locus_tag="ACG8E6_002219"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFS5062604.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4445..5017
                     /gene="rfbC"
                     /locus_tag="ACG8E6_002220"
     CDS             4445..5017
                     /gene="rfbC"
                     /locus_tag="ACG8E6_002220"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFS5062605.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            5039..6067
                     /gene="rfbB"
                     /locus_tag="ACG8E6_002221"
     CDS             5039..6067
                     /gene="rfbB"
                     /locus_tag="ACG8E6_002221"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFS5062606.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6165..7016
                     /gene="rfbD"
                     /locus_tag="ACG8E6_002222"
     CDS             6165..7016
                     /gene="rfbD"
                     /locus_tag="ACG8E6_002222"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFS5062607.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            7050..9083
                     /locus_tag="ACG8E6_002223"
     CDS             7050..9083
                     /locus_tag="ACG8E6_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062608.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9127..10407
                     /locus_tag="ACG8E6_002224"
     CDS             9127..10407
                     /locus_tag="ACG8E6_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFS5062609.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
BASE COUNT         3184 a         1748 c         2235 g         3252 t
ORIGIN      
        1 tttcttttag tatggttata agattttttc tgacaatttt attatcactg atattaacac
       61 ctgtattcaa aatcatttca gtacaaacag gtatggtcga caaaccaaat gaaagaagaa
      121 taaataaagt tccaatgcct tcagcgggcg gactgcctat ctttatttca tttgttatat
      181 cgactttatt cttattccgt aaaattatcc ctcaatcgta tattgtaccg atcttgattg
      241 cttctttagt aatcatagtc acaggagtgc tcgatgataa atatgagctg acacctaaac
      301 aaaaaagtat agggatcttg attgcttctc ttattattta ttttgtagca gatatacgta
      361 ttgattcctt cacgcttcct tttattggat atatccaact aggctggctt agttttcccg
      421 taacgatttt ttggatcttc ggtatcacga atgcggtcaa tctgattgac ggtttggatg
      481 ggctggcggc agggatttca ttgatcggtt taattacgat aggaatcatt ggatattttt
      541 tcctccatgc ttctacagtg tatattccag ttgttatttt ctgtcttgta gcaagtatta
      601 tcggtttttt tccttacaat ttttatccgg caaagattta tttaggcgat acaggagctc
      661 tattcctagg gtttatgatg gccgtccttt cactacaagg gctgaagaac gtgacttttg
      721 tttcatcgat ttctttgctg atcgtcatgg gagtgccggt aacagacacg ttttttgcta
      781 tcattaggag aaaagcaaac cgtgtttctt tttctacagc agataaaaaa catcttcatc
      841 accgactaat cagtttaggc tttactcata aaggtgctgt cctgacgatt tatgcgatgg
      901 ctttgatgtt ttctttcaca gctatggtca tgaattatac tggaagaatc ggaacgatca
      961 tattgattat tgccatgttg tttgctgcaa ttttattatt tgaattgatt ggtctgatca
     1021 atgagaaaca tcagtttatt ttaaaaacat tgcgattttt agggaataaa gagtaccgta
     1081 cgcaagtaaa acagaaatat ttgaagaaat ggaaccgtca tatagatgaa taacaaatta
     1141 aaccagccga tccttattag tattgtgacg gcaaacagtg ataaaatttt tcggacacta
     1201 gataatttta tttcaactgt aaaagatgaa acagcagtaa aaatccgaat ttttgacaac
     1261 aattcacttc ccgagtacaa aaaaagacta acagagtata ggaaatatcc ctacatcgat
     1321 atctattttc atgatgaaaa ttccggattt ggttatggac ataatcacaa tctgatagct
     1381 agcgattctc gctatggcat tatttgtaac ccagatattt tagtggatga agagacagtt
     1441 acagcattgg tcttgttatt gaaagaacat ccggaatgtg cgatgttagc acctaagatt
     1501 cttaatgaag atggaacaac tcagcacctc atccgtgagc agctggctgt ttttgattac
     1561 gttttgcgct ttgtgccttt caaatgggtc aaaaaaaagt ttgacaaacg actggctgtg
     1621 tttgaatgcc gaggattgcc tactgatgct aatagttatg tacggatgat ctcaggtagt
     1681 tttatggtag ctgatattga taaatttaaa gagataaagg ggtttgatga gcgtttcttc
     1741 atgtactttg aagacaacga cttgtgtttg agttttgaaa aagcaggtta caagttattg
     1801 tacacaccct tttatgcagt tatccatttt tacgggaagg gtgcgcatcg aaatgcaaaa
     1861 ctgtttcgcg tgtttttgac ttctatgatg aaatttttca ataagtgggg gtggcgtttt
     1921 ttctgatgaa accagaaata ttagtagtcg ttgtgcttta tcagcaacta ttcagtcaat
     1981 ctccagctta tggaccatta agcaaagcac ttagcgaaaa gcaaattcag cttattattt
     2041 acgataatag tcctcaaagg cagcaaaatg agctgtttga aaaagaaaat atcgtttatc
     2101 atcatgatcc gcaaaatcct ggtttagcgg tggcttataa ttttgcgttg tctcaagcag
     2161 atgaaggaac acgttgtctc gtcactctgg atcaggatac gaggctagtc gacgattatt
     2221 ttgaactatt gcgtaaagta acctttacag atgaatgtgt tgctgcagtt ccgatgattt
     2281 tttctgggag ccgacaagtt tctcccctat attcagatcg ttatatcaat cgtacagctc
     2341 aagcagtaga agttgaaggc ccaacagctg aacgaatcat ggcaatcaat tcaggtgttg
     2401 cctggtctgt caaatttcta aaaaagattg gcgggtttaa tcctgctttt tctttagatt
     2461 ttttggatca ttggctgttt tggaaagtgg atcagttgga aaaaactgtg gagatccttc
     2521 ctgtacgaat ggaacatgat ctttcggttt tagactatga aaaagtttca agtaatcgat
     2581 accgttctat tttacaagca gaaaatcgat tttatcatga atatgatcaa aggcatttat
     2641 ccagtcaccg acgacaatta ttgttaagaa cagctaaaca gttcttgacc gtcaaaaacc
     2701 ggcatatctg gcggatgact ttgcgctcat atgttaagaa ttggaaggtg tgaattcgtg
     2761 cgttcggttt gtatagctac ctttaatgga ggcagatatt taagagagca gttagatagt
     2821 attttatctc agctttcagc agaagatgaa gtagtcattt ctgatgatgg gtcagtagat
     2881 gatacatggg atatattaac tgaatacgca gaaaaagata agcgaatcca gcttttttcg
     2941 gggcctcgtc aaggattgat cgctaatttt ggttatgcga tccagcagac gaaaggagat
     3001 ctaatctttt tggcagacca agatgatgtt tggttggaga caaaagtgaa aaagatcact
     3061 gactattttg agcagaatcc ggatcagcta gtagtagttt ctgatttaat cattgtggac
     3121 agcgagctgc aagagatcca tgattcgtat ttttcgtttc gaaattccaa agaaggatgg
     3181 cttaataatg tgataagaag tcactatatt ggtgctggta tggctttccg gtcttcttta
     3241 aaacccgata ttttgccgat cccgtcagaa gtgccgatgc atgatatgtg gattggtttg
     3301 ctgggaggaa agaagactgg gttcctaaga gaaccgttaa ctctgtaccg aagacatgat
     3361 ttaaacgcga gtgagattga aacacattct aaattacggc aaaaaatttc ttggcgtgtt
     3421 catctgatta cggcgttgat caaacgaaaa tggtttaaac gttgataaaa gatgtttaaa
     3481 tccttaatat ttccatgaca aatataacaa ttgatgagat aataacaaag tgaaatttga
     3541 aatcaaaaag gagagcagat atccatgaag ggaatcattc ttgctggggg cagcggaaca
     3601 cgtctgtacc ctttaacaaa ggcaacatcg aaacaattga tgccgattta tgacaaacca
     3661 atgatttatt atccaatgtc tactctgatg ttggcgggaa tcaaagaaat tctgattatt
     3721 tcaacgccac aagatacacc acgtttcaag gaattatttg gaaacggaaa tgatttagga
     3781 ctgcagatcg aatatgcagt acaagaaagt ccagatggat tggcacaagc gtttatcatt
     3841 ggagaagaat ttattggaga tgatagtgtc tgcttggtat taggggacaa tatttactat
     3901 ggtggcggtc tgtctaaaat gttgcaacga gcagcttcaa aagatacagg cgcgacagta
     3961 tttggctatc atgtcaatga tccagaacgc tttggtgtag tagaatttga tgagaatatg
     4021 caagctttgt cgattgaaga aaagcctgct cagccaaaat caaattacgc tgtaactggc
     4081 ctttattttt atgacaatga ggtaatttct atcgctaaag gtatcaaacc ttcagaacgt
     4141 ggagaactag agattacgga tgtcaataaa gcatatttgg ataaaggaaa actatccgta
     4201 gaacttatgg gacgcggatt tgcttggctt gatacaggaa cacatgaatc attgcttgaa
     4261 gcatctacct ttatcgaaac aattgaaaaa cgtcagaact taaaagtggc ttgtttggaa
     4321 gaaattgctt accgtatggg ctacatcgat aaggaacaat tgctggccct tgcacagcca
     4381 ttgaaaaaaa atcaatacgg tcaatattta ttgaatttgg ccgctgaata aggagtcctt
     4441 acagatgaaa gtaacagata caaaattaac agatgtcaaa atcgtagaaa tggacgtctt
     4501 tggcgatcat cgcggtttct tcacagaaag ttattcaaaa gcaaaatttg cagaacatgg
     4561 attgaaatat gactttgttc aagacaacca ttcactatca gctgaagctg gtgtccttcg
     4621 ggggcttcat ttccaaaaag gagaagctgc acagacaaaa ttgattcggg tagtcactgg
     4681 agcagtttta gatgtagtag tggatatccg caaaggtagc ccgacttatg gtcaatggga
     4741 cggatttatc ttatctgaac ataatcatcg acagttgctt gtacctaaag gatatgccca
     4801 tggattcgtt actttgacac caaatgtgaa cttcctttat aagtgtgata attactataa
     4861 tgcagaagca gatgggggca tcgcatttga tgatccagat ttagcgattg attggccaat
     4921 tgatccatca agagcgatta tgtcggaaaa agatcaccgt catccgacat taaaagaatt
     4981 tgaagcagaa aatccatttg tttacggaga aatctaagaa ttaaggagaa attttcttat
     5041 gaaaaacatc attgttaccg gaggtgccgg attcatcggc tctaatttcg ttcattacgt
     5101 ggtaaacaat catccagaag tgcatgtcac tgttttggat aaactgactt atgcaggaaa
     5161 caaagagaac ttggctggac ttcctagcga tcgcgtagaa ttggtcgtag gagatgttgc
     5221 agatgcagaa ttagtggacc gtctagtaaa agaagcggac gcagttgttc attacgcggc
     5281 agaatcccat aacgataatt cattaaaaga tcctttccca tttgtacaaa cgaatatcat
     5341 tggtacgtat acacttttag aagcttgccg caagtatgat gtacgctatc atcacgtatc
     5401 tactgacgag gtttacggcg atcttccatt acgtgaagac ctgccaggac atggggaagg
     5461 cgaaggagaa aaattcactg cggaaacacc atataatcct tcaagtccat attcttctac
     5521 aaaagctggt tcagatctgt tagtaaaagc atgggtgcgt tcttttggct tacgtgcaac
     5581 catttcaaac tgttcaaaca actatggtcc ttatcagcat attgaaaaat ttattcctcg
     5641 ccaagtaacg aatatcttaa gcggtattcg tccaaaatta tatggcgaag gaaagaatgt
     5701 tcgtgactgg atccatacga atgatcattc ttcagcagtg tggctgattt tgacgaaagg
     5761 cagaattggc gaaacctatc tgatcggtgc agacggtgaa gaagataata agaccgtgat
     5821 ggaattgatc ttagaaatga tgggtcaacc aaaagatgcg tacgaccatg taaacgaccg
     5881 tgcaggacac gacttgcgtt atgcaatcga ttcgacaaaa ttacgggaag agttaggatg
     5941 gaagcctgag tttacgaact tccgtgatgg gttggctgac acgatcaaat ggtacgaaga
     6001 acatgaagac tggtggaaaa aagaaaaaga ggccgttgaa gcagcatatg ccaaaaatgg
     6061 acaataatac agagaaaatg tcactgtatt aatggaaaaa cacatgtgag gcaggcgctc
     6121 gcctgtctct tttttaaaat aaaaatcaag gagcggataa tcatatgttt ttattaactg
     6181 gaggaaatgg gcaactaggt acagaattgc gccatttatt agatgaaaaa aatctgcaat
     6241 atgtttcaac agatgcaaaa gacttggata tcacagatgc cgagaaaaca atggcttaca
     6301 tcacagaatt aaaacctgat gttattttcc attgtgcagc ttacacagca gtagataaag
     6361 cagaagaaga agcaaaagaa ttagatgaga agatcaatgt tgatggtaca agaaatgtag
     6421 cacttgcagc aaaagctgtg aatgctcaat tcgtctatat cagtacagac tatgttttcg
     6481 atggaaaaaa caaaaatgta gaatacgaag tagacgacca aacgaatcca ctaaatgaat
     6541 atggtcgtac gaaattatta ggcgaaaaag cagtagaaga gattttagaa gactattata
     6601 tcatacgaac ttcttgggtt ttcgggatct atggacacaa tttcgtcttc acgatgcaac
     6661 gcctagcgga aacccgcgac aaactgactg tagtaaatga ccagtttggg cgaccaactt
     6721 ggacacgtac tttggcagaa tttatggtat atgtcatcga agaaaaagca ccatttggta
     6781 tttatcattt gtcgaacgaa aatagctgtt cttggtatga attcgcaaaa gaaatattga
     6841 aagatacaga tgtagaagtt gcacctgtta cgtcagaaga atttccgcaa aaagcaacac
     6901 gtccgcagta ttcagtcatg agcctgaaaa agacagaaga gttaggattt gtcataccaa
     6961 cttggcaaga agcacttgcg caaatgctgg aagctgccaa aaaaatgaat aagtaatttc
     7021 cttttatcaa atagaatttg gagcatgata tggaaaaaca aacaagaagt gcacgacgaa
     7081 aagaaaagcc aaaagcgaac caggcagatt cgaatttggt gcttgacctg tttctaaaac
     7141 tccgctatct tattggcgtt ttagtcatcg tggtcatcgt tacttttaat ttaaacggaa
     7201 gttcacttgg tgtttgggat aaatatgtgt cgcaaagaga cgatgggaaa aaaactgatg
     7261 tgatttttgg acagaatcgg gaagtgcggt cagacgaatg gctggtgcag acaccttttt
     7321 acctctcaca agctgaagag gactttccgt tggtgaacga ggattattca ttaaacggac
     7381 aaaacatgat cattgcgtat aattctcctg caaaggatat tacggttatc ggaaaaccat
     7441 tcaactgggg attctttttc ctcggaagag atcgaggact ttccttttat tgggcgatga
     7501 aattagtcgt catggtcttg cttggattcg aagtactgat gatattaacg aagagaaaaa
     7561 aagctctatc gctaatcggt gcatttgcgc tgacattttc tccagcagtc cagtggtggt
     7621 tcatgcaaca tgtgggcgat ttgattttct ttactttggg actgatgact gctttttatc
     7681 attatttcta caatcatgat aaaaaatggc tacgaacgtt gatgatgctt ttggtggtta
     7741 ttttcggtat tgggtttatt ttagtgattt acccagccca tcaagtgatg ctggcgtact
     7801 tgctgatttt ttattttgtt ggcctgctta tttactatag caagaaagtt tcatgggatc
     7861 tgttggatgt agtgttgatt ggaggggcag ttttatttat tggcggagtc atggttcatt
     7921 tctggctaac gtcaaaagac gcattgttag cttctttgaa taccttatat ccaggaaaac
     7981 gggtaagtac tggaggaaag tggacgattg gcgaattttt ttctttcctt acaaattgga
     8041 aaattccttt tgaagatatc aactattcga ataactctga agtggccctg ttctatcact
     8101 tttttccaac tgtattccta gcaagtccat ttgtgttatt tggcaaaaaa gatagcgaac
     8161 aaaagttact gggacgtatt ttgatgattt tttgtctgtt ttctatcttt tggattaccg
     8221 ttgggttgcc aaaatggcta gcagaaatca ctctgctgtc ttatgttcct ccattaagag
     8281 ccatccttac ttttagtttt gcggcttgtt tattatcgat ctggtttatc gcttatattt
     8341 ggagagaaga tgttattcct atttggtata aattcatttt gttggcagta aatgcatggt
     8401 cttatttcta tgcgattacg cattcgaaaa tagataatta tttttcggat tttgaaacca
     8461 tcgcagttgc tgtgttggga acagtgattt tagcatttgc attatttggc tggagacgct
     8521 tgttttacct cgcttttgcc gccttagtta tcgtatctgg ctttttcgtc aatccagtcg
     8581 tagaaggtac aggagcgatt tttgaaaaaa cactggcaaa agaaatccaa aaaatcgatc
     8641 aacaagatcc tggtaaagtt tggttgtctg aagatgaatt atataacttc accccaacat
     8701 tgggagtgaa atccttcaat acagtacgct tttatccaga tatagctgca tggaaggaaa
     8761 tcgatccgaa agaagaatac gagaagtatt ataatcggta tgctcataca agagctttta
     8821 tcgctaagga agatacaaag ttcattttag atcgtccaga tatgttttcg gtcacgttga
     8881 attttgaaga cgcagaaaaa ttagggatcg actatgtgat cagtaaacga ccattaactg
     8941 attacaacca attatatgaa gcgcagttca cgcagctata tggaccggat aaagacggtt
     9001 atatgatttt taaagtagaa tatccggatt atccagatgt accacaaaca caaactcagg
     9061 aaaatcagct agtcgtacag taataggcta gctgagaata atcctgaaat attagtagaa
     9121 aggagagtgg agataattca agcaatcgta caaaaatttg tcaacaaaat gagtgtcggt
     9181 ttcttgcttt tgttgctgac gctagtcttt tttgtttgga cagcagggtt aggttttcca
     9241 gcaactttgt ttatatataa caatagttca ttacttatcg cttttagtgt ggctattttg
     9301 ctcattttaa atgtttataa agcaaaaggc gtcgattttc tttatctagg tgttgcgctt
     9361 gcccttttca ttttcttttc ttactttcgg gaattaagaa atgagtcttt atttggagat
     9421 ttactgtttc ctttgattat tttattttgt ttagtaatta aatggatcga gtggacaaaa
     9481 tgggatcgcg gtatttatct gattgtcttt acggtcttcc tcggaatcac gttatatcgt
     9541 gtatttacag agatccaagt accagaagga caaagcatct gggctccagg caattcgctt
     9601 tctgatattt ggatcaatac gaatacaatc ggaagttctt tgatgactct tgctttattg
     9661 atcagtggat ttgccagttc ttttgaacga tggtatgtac gaatcatcgg tgcagtagca
     9721 gttgttgcag cttttctcgg gatttgggtc tgccaatcta gaggtgcatt agtcgcgttg
     9781 attgtttttg cgatattgga tcttttacct aaatcgttga tgcgtgtcat tcgtgcgcca
     9841 tttattgctt acacggttac gattctttta gctttgccga tttcttatct ggcggctgtc
     9901 tctgaaaaag tcaatttgtt taccggtcga gaagatattt ggcataaatt ctatcagacg
     9961 ctaggagaaa aatctgaaca agttttacta ggaatgaaaa catttatctt ccaacgagga
    10021 aaccaatttt taggaaacca caatagttat aattctattt tgaatatcta tggattgata
    10081 ggatttggga ttgctacttt gctgcttatc ctttttattg gtcgcttaac gttgaaagca
    10141 gatttatcca acgggcagat gacttttatc tgggcctttt ttgcggtgat gatgcagtca
    10201 tttatggagg atacattgac ttcataccca tgggttccga ttatttatat cctgttggct
    10261 atggccgttc atcgatatga tgaaccagaa gttgcggagg aattctatgt tatggaagag
    10321 gcatatgacg atatgactta tgatgataca tcttatgagg aacaaccaga tagcgctcat
    10381 tcttccagga tccaacgcta tcattgatag gcacaaaat
//
LOCUS       DAZUMM010000085        10323 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000085 DAZUMM010000000
VERSION     DAZUMM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.087"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..321
                     /locus_tag="ACG8E6_002225"
     CDS             172..321
                     /locus_tag="ACG8E6_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFS5062610.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            332..1846
                     /gene="dltA"
                     /locus_tag="ACG8E6_002226"
     CDS             332..1846
                     /gene="dltA"
                     /locus_tag="ACG8E6_002226"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFS5062611.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1843..3048
                     /gene="dltB"
                     /locus_tag="ACG8E6_002227"
     CDS             1843..3048
                     /gene="dltB"
                     /locus_tag="ACG8E6_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFS5062612.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3093..3326
                     /gene="dltC"
                     /locus_tag="ACG8E6_002228"
     CDS             3093..3326
                     /gene="dltC"
                     /locus_tag="ACG8E6_002228"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFS5062613.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3329..4597
                     /gene="dltD"
                     /locus_tag="ACG8E6_002229"
     CDS             3329..4597
                     /gene="dltD"
                     /locus_tag="ACG8E6_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFS5062614.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4738..5820
                     /locus_tag="ACG8E6_002230"
     CDS             4738..5820
                     /locus_tag="ACG8E6_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFS5062615.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5820..6623
                     /locus_tag="ACG8E6_002231"
     CDS             5820..6623
                     /locus_tag="ACG8E6_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS5062616.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6693..7433
                     /locus_tag="ACG8E6_002232"
     CDS             6693..7433
                     /locus_tag="ACG8E6_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5062617.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7444..8448
                     /locus_tag="ACG8E6_002233"
     CDS             7444..8448
                     /locus_tag="ACG8E6_002233"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFS5062618.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8493..>10323)
                     /locus_tag="ACG8E6_002234"
     CDS             complement(8493..>10323)
                     /locus_tag="ACG8E6_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062619.1"
                     /translation="NIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSPADSNQ
                     MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNH
                     QWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPP
                     KVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSC
                     FALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIF
                     CTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVAC
                     TPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRR
                     QVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKI
                     LEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLL
                     IHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDD
                     QERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3321 a         1703 c         2087 g         3212 t
ORIGIN      
        1 tttaaaagta cgagaaatta taaagaaaaa agcagtgatt tgatgtgcta tttttataaa
       61 agctatgtga caaaactgtt agtaaaaaaa cggtttctgt aagctagaca catgtttctt
      121 tttgtcaaaa ttagttacac tttgtgtgag gttatatgag gagggaatcc aatgaacaaa
      181 tggcgttcta atccaaaatt gaattattgg ggaaaattca tcattcgaac aattttttat
      241 agtgcagtac tgctgttcct gatttatctg tatcactata aaaatattca aggaggaact
      301 tttatctata acgaatttta ggaggaagag tatggaaatc aaaacgatta ttgaagcaat
      361 cgatgattgg ggaataaatg aacctgatcg agttgcttat caggcggatg agacgcatac
      421 ttttggtgaa ttgaaacaag catcagacgc acttgcttat tatttagaaa aaaaggtaga
      481 aggcgatgga ccaattgtcg tttttggcaa tttggagttt gaaatgattg tctcatttct
      541 aggggttgtc aaatctggcc atgcctattt gccgattgaa gagcatacac ccaaggagcg
      601 tatcctatcg atctttcggg tggctaaacc atctatggtc atcagtatcg gggactggat
      661 agaagatatt ccttcggttc ctgtcataac aaaaaaagaa tttcaagaaa tcaagaagat
      721 accagtagga ttttatccca gaaatagcgt aaaaggtaca gaaaattttt atattatttt
      781 tacttctggt actactggag aaccaaaagg cgtacaaatc agtcatgata atctagtcag
      841 ctttgttcaa tggacgatgg aagactttgg gatcaagcct ggtatgcatt tcttagccca
      901 agcgccattt tcttttgatt tatcggtatt ttctatttat cctgcattag tttctggcgg
      961 aatgctgaag ccgttaaata aagcagttgt tcaagacttt cgtcaattat tcgcgacatt
     1021 gccagccttg aagttgaatg tgtgggtttc tactccatca tttatggata tttgcttgat
     1081 ggaacctaca ttcaatggag aaaatgtgcc ggaattggac atgtttttat tttgcggaga
     1141 agaattgacg aaaaaaactg cagaaagtct acttgatcga ttcccaaatg cacggatata
     1201 taatacttac ggtccaacag aagcaactgt agctatttca agtattcaga ttgaccagca
     1261 tgtactagat agttatgacc gactaccgat tggctacgta aaaaaagaca cgaaagtttc
     1321 tattgtccaa gaaggtaaac ctgtttcaaa aggagaaacg ggtgaaatca ttattgcagg
     1381 accaagcgta tcaaaaggtt acctgaacaa tcccgaaaag acagctgctg cgttcttttg
     1441 tcaaaatgac ttagcaagtt accatacagg ggatgcaggt agactagatg aatatgggct
     1501 tttattttat gaaggacgta tggatcagca ggtcaaatta catggatacc ggattgaact
     1561 cggggatatc gaacattatc tgctccaaga caaccgaatc aaacaagctg tcgtcgtacc
     1621 aaaataccaa ggaacaaaag tacagcagct ggttgctttt gtcgtattag aaaacaaaac
     1681 gaaacagccg gattttcagt tgactaaatc tatcaaagaa caattactag aagttgtaat
     1741 ggattatatg attcctcaga agttccatta tgtcgatctg ctgcctcaga cagttaatgg
     1801 aaaaatcgat cgaaaaaaat taacagctga ggtgaatcct gaatgatgtt acctcatatg
     1861 atcccttatg cagagccgtt ttattttttt cttttagcca ttgcgcttgt accgatcgtt
     1921 ctatctttat tgatatggga aaaaagatta ccagtttatc aatcagcagt gacacttttc
     1981 tttttatttg tcagctttgg aggtaattat tggcaacagg gagcggcgct gattgggtat
     2041 gttttgtggc agacgatact tgtatggggc tattttgttt atcggcaaag gaaaaatcag
     2101 acaggatggt tttttgccgc cgttatttta gcgataattc cactgttttt aacgaaagtg
     2161 actccttttt ttgaacatgg aaaagcatcg ttacttggtt ttttaggaat ctcttattta
     2221 acctttaaat ctgtacaaat gatcatggaa ataagagatg gcatgatcaa aacatataag
     2281 ccatttcgtt atatccagtt tctgctgttc tttccaacaa tctcttctgg accaatcgat
     2341 cgctatagga gatttgaaaa agatcagctc catccgcctg ttcgtgataa atatgttgat
     2401 cttttgggaa aaggcgtcca aaatattttt gtcggttttt tatataaatt cattatcggt
     2461 tattattttg gtcaagtact gcttcctgta gtgggacgca ttgccttaca gcatggtggt
     2521 atttcatggg cattagtagg ttatatgtac gtgtacagta tgtatttgtt ttttgatttt
     2581 gctggctata gcttatttgc agtcggtaca agctacatga tgggatatca gacaccaatc
     2641 aatttcaata aacctttttt atcatggaat atcaaagaat tctggaatcg ttggcatatg
     2701 acgctgtcct tttggttccg tgattatgta tatatgcgat tgatgttctt catgatgaag
     2761 aaaaaatttt ttaaaagcaa gatcgtaatg tcaaatatcg gttattttgc tttgtttctt
     2821 ctaatgggta tatggcatgg attgacgtgg tactacatcg tatatggact gtatcatgct
     2881 tgtctgatct gtctgacaga tgcatggatc cgatttaaga agaagcataa agagcagttg
     2941 ccttctaaca aatttacgca tggcttagcc gtatttttga catttcaagc agtatgtttt
     3001 agcttcctga ttttttcagg atttttagat aaactttttt ttagctaacg aatagtgaca
     3061 tattaattta tataacaaag gagaaaagga aaatggaaga acaaatttta tcaatcttag
     3121 aagaaataac aggaacagat gaagtaagaa aaaatcgtga tattgatcta tttgatgaag
     3181 ggttgcttga ttctttagca tctgttcaat tattagtcga gctagacgga gagctggata
     3241 tccaagtccc tgtatctgaa ttcgagcgag aggattggag cacaccgaat aaaatcattc
     3301 aacaagcaac tgcattgaag ggttgagtat gaaaaagaaa ctttttgcta ttttcggccc
     3361 ggttgtttta gctgttggct tactgtttgt gttttttacc tcctcctatc aagtagatat
     3421 ctctaatcca gaaacgatta gacgtgcctc ttcttccatg tcagtgaacg tgttgaaagg
     3481 agatgcgata aaaaattatg cattatctga aaaacaatat attccttttt ttggctcaag
     3541 tgaattaagc agaatcagtc catttcatcc gtctgttctg gcagaaaaat accagcgaaa
     3601 ctatcgtcca tttttgctgg gagcacccgg gacacaatcg ttaagccaat atatgatgat
     3661 gcgttctgca ggagatgcta tgaaaaacaa gaaagtcgtc tttattattt ctcctcagtg
     3721 gttcgttaaa aatggtgtaa aaactgatta ctttaatacg tattattctg aacttcaaac
     3781 atacgactgg ttgttttcaa tgaagaaagt aacgcctgct gatcgctatt tagctagaag
     3841 attattaact ttctcaaaag taaaagagaa tgatacgttg acggcgattc tgcaaacgat
     3901 caaaaaaggg aagctacctt tacctgaatc tttgaatcag ctgcgcagcc aatggaacat
     3961 gctgaagaga gaggatgaag tattcagcaa tattgggttg aaagatcgac agcaaaaaat
     4021 cgatcatgag tcaaaacgat taccaaaaca ataccaagaa acagaattat ccattttagc
     4081 aaatcaaatt ggagaacgag aaacgacgaa taatccattc gggttgaaaa acgattttta
     4141 tacgcatcgt atccgagccc acgagccaga gctcaaacag tcacaaaaaa attgggatta
     4201 tcgtttttcg ccagaatttt ctgattttca gctggtcttg gatcaattag caaaaaacca
     4261 taacgaagta ttgttcatca tcccaccggt taacgagaaa tggagcgatt ataccggact
     4321 atctcaagaa atgttacaag gatttgccaa aaagatcaaa tttcaattaa atagccaagg
     4381 attcaatcga atagctgatt ttgtcaatca agcgggtacc aactatttta tggaagacac
     4441 gatccatcta ggctggaaag gctggttggc tgcagatcaa cagatcagac cgtttttaga
     4501 agaaaatcat attactgcta gtaagtatca tcttgacgat gcttttttta gcaaatcttg
     4561 gcaacatcaa ataccagata aactacaatt aaaataaaga aaacaggcga gtgttgctcg
     4621 cttgttttct ttatttttga atattctgaa taaaagataa aaataccaag tttgcctgaa
     4681 caaaaatcat aagcaatggt ataatcacaa tatgaatata gaaatggggg aaaacatttg
     4741 gatacaaaag aagaacagct acttattgat ttaacttctg caaaaggagt acctggaaat
     4801 gaagaagagg taagagaagt attcagagaa tatgccaaac cattcgctga tgatattttt
     4861 tatgacggtt taggcagtgt gattgccaaa catggtgctg gcggaggccc caaggtcttt
     4921 atttctggac atatggacga agttggtttt atggtaacaa aaataaccga aaaaggattc
     4981 ttggaattcc aaactcttgg aggttggtgg gggcaagtca tgcttgctca gcaagttgaa
     5041 atcaaaacac gtgaagggaa aatcgtccac ggtgtgattg gttccaaacc accacatgtt
     5101 ttaacacctc aagtaagaaa taaaccttat gaaataaagg atatgttcat tgatattggc
     5161 gcatcaagcc aagaagaagc caaagagtgg ggcatacgtc ctggagatat ggtcactcca
     5221 tatattgaat acaaacgaat gaatggcagt aaatatttac tggcaaaagc atgggataat
     5281 cgtatcggga cagctgtttc tctgcgagta ttagagaatc tatctaaaga agcacaccca
     5341 aatgttttat tcgcaggaag cgatgtgcaa gaagaagtag gcttgcgagg tgctcgaaca
     5401 agtacacacc ttgtaaatcc cgatattgct tttgctttag atactggtac agcaggagac
     5461 actccaggaa tgacaccaaa agaagcagat tcgattttag gaaaaggccc acagattttg
     5521 atttttgatg catctatgat cccgcataaa aaattattga attttgttat tgatgtagca
     5581 gaggaaatgg atattccatt ccaatataca gtgatttctg gcggaggaac tgatgcagga
     5641 caaatgcatt tgacaagaga tggtgttccg tctgtagcca ttaccgttcc tgttcgttat
     5701 ctgcattccc atacatcagt gattcatgaa gatgattatt tgaatacagt gaaactagtc
     5761 acagaattag tgaaacggct tgatagtgaa acagttgaaa aaattcggag ttatgaataa
     5821 tgaataatag aatacctatt tctgaatatc ggaaaaaagc acgagtttct ttggaaaatc
     5881 aatggggaat caatacttgg cttgttttct tatctataat atttacgagc gtcatccaga
     5941 acttttttga cggtttcagt ccttttccgg aagaaagcat gcagcagcac ttattgagtt
     6001 ttctagtcga taatttactt ttattcgcat tcaactatgc attgttttac attgcgctag
     6061 tagttgtacg gggtggcaga ccaaaaagta accttttatt ttcagttttc caaagaaagt
     6121 attttggttc gatacttggc atcaatttga tcaattcagt catcaactgg ctactcaaca
     6181 gtttaatctt tctgcccatg tttttaatca gcggagtgag tacttatatg cagctgcttt
     6241 tcagttcaaa tgcggtacct acgggtgtca tgccagaaga ttttcttgat atcagctttg
     6301 ttttatcgat gatagggatg atttttgttt cagcactcat catgagtatt gttggcggtt
     6361 tattccaatt tgctgcatgg acaaagttcg actatcctga tttgtcggtt attcaatgtt
     6421 tgaaatatgg ctggttttta ttgaaagata gaattggtac gtatattttg ttgcagctgt
     6481 cttttatcgg ctggtatttg ctaggagcaa tcgctttgtt catcggtctt ttgtgggtca
     6541 ttgtttatgt gaatgtgtca gttgcagaat tttatgaaca agcacggatt gaaaaaggag
     6601 gtccagacgc ctattttact tagcatgtaa gcagtttatt catcaaaaaa ataacttttg
     6661 tggtaggata aatctttgag ggggaatgta cgatgaaatt aactgtttta ggatgcttag
     6721 gagcgtatcc ttataaaaat caagggacaa caggttattt gcttcaatct gacgggttca
     6781 atttgttgat cgatgcaggt agtgctacgc tgatcaaatt acaagaacac cttgatccac
     6841 ttgatctaga tgctgtgatc cttagccatt atcatcatga tcatattgct gaccttggtg
     6901 tattacagta ttactggcaa cttcatccta agagaaatcc ggagacattg ctttcaattt
     6961 atggacacac aaaagattcc attcactttg aagaattgac gttggaaaat gtctcaaaag
     7021 gacatcctta ttttgaagct gaagaattaa aaattggtcc ttttctgatt acttttatgg
     7081 aaacaatcca tccagtagta tgttacgcca tgcgtatcgt agaagaaaca acggggaaag
     7141 tattcgtatt tacaggtgat tcaggttatt tggaaagttt cgctgatttt gcgaaagatg
     7201 ctgatttgtt tctagcagat acctatcttt ttgaaggaaa tgaacatcat catgcccatt
     7261 tcacttcaaa agaagctgga gaacttgcga aatcagctaa cgtaaagaaa cttgtactta
     7321 cacacctacc tcagtttggt gatttagaac aattgaaaaa tgaagcgcaa aaggcagcag
     7381 gcaaagggat tgatatagaa cttgctgcag ttgacaaagt gtttaacatt taggaggaag
     7441 aacatgattt ttgtaccaaa tgaaaaccac gacccaagaa tcaatttagc aattgaacaa
     7501 tatttgctgc aagaaatgcc tttagatgaa cctattttgc tcttttatat caatgaacct
     7561 tcaatcatca tcggcagaaa ccaaaatacg cttgaagaaa tcaatcgaga ttatgtggaa
     7621 gaaaatggca ttcatgttgt ccgtcgctta agtggaggcg gagctgtcta ccatgatttt
     7681 ggaaacctga actttagttt tatcatgcct gatgatgggg attcattccg tgactttgcg
     7741 aaagtaacac agccaatcat ccaagcactt catgaattgg gtgttgctgg ggcggaattg
     7801 aaaggccgaa atgatctagt cattgatggc atgaaatttt ctggcaacgc gatgtatgct
     7861 accaacggtc ggatgtttgc tcatggaaca attatgttcg actctgatat caatgaagta
     7921 gtcaatgcat taaaagtaaa aaaagacaaa atcgaatcaa aaggaatcaa atcgatccgt
     7981 tctcgtgtaa cgaatatcaa accatttctt ccagaagaaa agcaaaaaat gacaacagaa
     8041 gaatttcgtc aagaaatctt gttgaaaatt ttcggcgtag agtcagtaga agaagtaaaa
     8101 acgtacgaat tgactgatga agattggaaa aaaatcaatc gaatctctga ggaatattat
     8161 cgaaattggg attggaatta cgggaagtct ccagatttca atttctctcg ccaaaaacgt
     8221 ttttctatcg gttcgattga agtccatttg aatgtctcag aaggaatgat caaagaagca
     8281 aaaatctttg gcgatttctt cggactagga gatatctctg acgtggaaaa aaaattggtt
     8341 ggacagaaat atgataaagc atctctatca aaaatagtca gccaaattga tgtgaagaaa
     8401 tattttggtg cgatagaacc agaagaattg cttaccttac tttattgaaa ataaaaaata
     8461 gcggaatcca catatatgga ttccgctatt ttttatacag gattcgggta atttcgagag
     8521 agttgaacag tgatccgatc aatgaaagta tctttttcct gcgttagtgt tcctgtgtaa
     8581 cgatccgcta cgtctttcat catcattaag tcaaaacgtt cctgatcatc taattcttct
     8641 gtgatgcctc gactaaatag gtttgtatcg taatgtgcac tatgttcgca acaaatgacg
     8701 aaatgttcct cttgttcgtg tatcgacaaa taaatttttc gatacagtgg attttcgatg
     8761 gcatgagttt ctctaaagga atgctctaaa gcgtggatca aaagttgagc cagatcgtca
     8821 tcgttgatag gaagttcttc gggaagatct gcttggaagg ttatctgaat attccgtttg
     8881 gcagctaact tctggtaacg cgctaagata agatttgtca cctggtggtt gcaaaaagaa
     8941 cacagcattt cgaaattttt tgcttcttct aatatctttt ccacatagtt tttagctttt
     9001 gtttggtccg tatgcattaa atcttgcaga ttctccatct cttggacaaa ttccaggcga
     9061 agtgtattta cttcttggat atgctgtcga agctgctcat aatgttgctc caacaaatca
     9121 tttttgattt ttaagaaatc tacttgtctc cgatctttgt cggtatttaa gatgtgatcg
     9181 attagattgt agctgataat aacgattaga atgataatat ataaaatcgt agaccaattg
     9241 atcgttgttt gatagatacc taatgccgtt tggatgtaaa tcaagcaatg gataatggct
     9301 accaagacga tccaaggtgt acaagctaca taaaaacggt tattcttata tgcttcgcca
     9361 atgcatgcaa gtgaagtgac taaagtgctt ataacactag caatattagc aatcggcatt
     9421 aattcaggaa gttctgcagg agtaaaagtt gcataaagca gaacgccgat aaaaatcaaa
     9481 agttggaacg tacaaaaaat tccgtaccat tttttatagt aaaacattcg gctccaatag
     9541 tagcagatga tgaaagctga cgttaagata ataaataaat gagataaaaa agaatgaacg
     9601 aatggttcaa aaagaatacc acttaaaata tcttctgata cagcttcaaa tcctaatgcc
     9661 aaagcaaagc aacttaagag aataagtgag tattcaagac gtttttgttt atacatcaag
     9721 tagatcgtat agaaaaaggt ccctatagcg ataaaaacag cgattatcat cgtcaatact
     9781 tgcggcactg attgtgaata aataaaacta atcaatggtc cagttgttcc tagataaact
     9841 ttcggtggca gtccagcata gttctgataa ggagatgaga ctgtaagggt caattttttt
     9901 ccgatataat ttgctggtaa atctatcaca gccaatgaga gtccgggatt gctttcttcc
     9961 tgataatctt tccgttggta taaaatcttt tcgcctagat atacagtaat ccattgatgg
    10021 tttcctttga ttagtaaatt tgcttcgtcc aacggttttt caagtgtcgt ttccataatg
    10081 aatgtttcgt ctttatccac ggtaggaatc gtacgagtgt acttgctgtc aaacttaaaa
    10141 tcaggatcat cttttgtata gaaaacccac gaatctttta tttctttcat ttgattgctg
    10201 tccgcaggag aaaaaataaa ataaaagaaa taagcaattc ctgttaaaaa taacaatgat
    10261 aatataatga ttatgctcca ctttatacgt aaataaaaag attctttttt attatatatg
    10321 tta
//
LOCUS       DAZUMM010000086         9947 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000086 DAZUMM010000000
VERSION     DAZUMM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.089"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(411..635)
                     /locus_tag="ACG8E6_002235"
     CDS             complement(411..635)
                     /locus_tag="ACG8E6_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062620.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            826..3603
                     /locus_tag="ACG8E6_002236"
     CDS             826..3603
                     /locus_tag="ACG8E6_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5062621.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3684..4103
                     /locus_tag="ACG8E6_002237"
     CDS             3684..4103
                     /locus_tag="ACG8E6_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5062622.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4115..4585
                     /locus_tag="ACG8E6_002238"
     CDS             4115..4585
                     /locus_tag="ACG8E6_002238"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5062623.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4602..5381
                     /locus_tag="ACG8E6_002239"
     CDS             4602..5381
                     /locus_tag="ACG8E6_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5062624.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5368..6189
                     /locus_tag="ACG8E6_002240"
     CDS             5368..6189
                     /locus_tag="ACG8E6_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5062625.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6206..7255
                     /locus_tag="ACG8E6_002241"
     CDS             6206..7255
                     /locus_tag="ACG8E6_002241"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5062626.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7268..8272
                     /locus_tag="ACG8E6_002242"
     CDS             7268..8272
                     /locus_tag="ACG8E6_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5062627.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8905..9924
                     /locus_tag="ACG8E6_002243"
     CDS             8905..9924
                     /locus_tag="ACG8E6_002243"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS5062628.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3341 a         1644 c         2081 g         2881 t
ORIGIN      
        1 aaagaaggag tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga
       61 ggaataaata atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa
      121 cgaagacaac ctcccgacat caagtcaaaa gctacttgag attttgatct gaaaataaaa
      181 aaagaaaaca aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa
      241 actaagagtg gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc
      301 tgaaagcatc ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat
      361 aaggatttgt aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta
      421 tggaaggatt tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct
      481 atatattgcg tatctacgcc aataaaacga gtttgttctg tcatagccat ataaagcaaa
      541 ggcaaaaata caactacagc gagataagat ccgatgattt ctcgaaaagt tttccaggaa
      601 gtatgtttcc tgtttttata tttatccgat ttcattgaat agtcatagcc tccttattct
      661 ttcatatgtt gaaattccag tataaaaact ggaaggaact gtacgttttt tagaataaaa
      721 attataaggg ataagtaggc tgctgtctat aataagatat atttttcaat tctttattaa
      781 agattttatt gacaaaatga atccgttttc tatgatggag gaggggtgaa taagttgttg
      841 aagaaggaaa ttttatgtta cttagagaac cagacagcct ttttcgattt agaacatatg
      901 aacgaggtct ttacagcaca aaggttagct gatcattttg gggtaaaaag aaacacagtc
      961 agtcattatt tgaaccagtt aaatgatgag caactactag tcaaaatcaa ttctcgacca
     1021 gtgatttatt tccacaaaga agcttttgaa aaacaatttt ttaagttacg cacgaatttt
     1081 tatttttcta tcgatgatct caaagaggaa cagccttttt tcgcgcaacc aaaagactta
     1141 ttctctttta tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca aatcaaaaca
     1201 gctctttact atccagatgg cggattacca cttttgatta caggcgaaag tggcacaggt
     1261 aaaagttatt tagttaattt ggtctatcag tattgtctgc ttcatgatct gctagaagat
     1321 tctgcaccat tcatcacttt taattgtgca caatatgcag ataatcctga acttttgact
     1381 agcaatttgt ttggacatgt taagggagcg tacactggtg cagaagaaaa taaaaaaggg
     1441 gcttttgaag cagcagatgg tgggatcttg tttttagacg aggtacatcg cttaacacca
     1501 gaaggtcagg aaaaactgtt cacctatctt gatcaaggtg tgatttatcg tatgggagaa
     1561 cctaatttgt cacgccggat caaaacacgt ctcttttttg ctacgactga agagattact
     1621 agtcatttct taacaacttt cattcgaaga atacctatcc gaatcgaact acctgcactt
     1681 aatcagcgta gtcgtaatga acggctagaa ctgatttact ctttttttct cgaagaacaa
     1741 agaaaaatgg ggagcccttt gtcagtcagt ggaccagctt tgtcactttt aacaagtcag
     1801 aaattatccg gaaacatagg agagctgaaa aatatcgtca aagtttcagc agcaaaagca
     1861 tatgctgaac agcgtgaaca gcctgaaatc catgtcacga tccatcattt gcaaaaagaa
     1921 ctacttgcgc agcctattac taaaaataca gcacagcaag gaatctatat tacgcaagac
     1981 cagaccttag agcaattaat ggagaagcaa cagcccgaac agcagcgaat cgttaaaagt
     2041 tttgagcaaa tcctaattga tttcaagaaa aataactgtc tgctatattc ttgcgaagga
     2101 aaattaaagc aagaagtgat tcaattattt gattttttgc tgtttgagac cagtagacaa
     2161 gaaaaacacg agctgctggt ttattggaca caatctatcc gagagacctt tcgacaaatg
     2221 gaaactgcct atcaaatcaa attcgatgga aacagtgtct atgccttgag ttactatctc
     2281 tatcagcgaa gcactatcaa atggttaccg gaagaacatg atattatcca actaatcaaa
     2341 gaattagaac aacaagtttc tcagtcttat ccatctagtt atcactatgt ccaacgaatt
     2401 ctggaattaa gtaagatcaa attagatatt gaagacacaa gtatggatcg gattttactg
     2461 acgatctatc taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa agcgatgatt
     2521 gctgctcacg gttatgcaac tgctagcagt atggcaaatg tagtaaatcg tttattacaa
     2581 gaggatctat ttgaatcatt tgatatgcca ctagatgtca caccacagca gattgcttca
     2641 gaaatccttg attattgcga aaacagcgat gtttcaaatg gattgatcat attagtagac
     2701 atgggatctc tgaaagaaat ccatcagttt tttaaaaaac agttatccgt accgcttttg
     2761 attttgaata atgtgacgac acctttagca atcacagtag gggaatgcct gcaaaaaaat
     2821 gtatcattag aagagatagc agaagagact gttcgacaga tccagccaga atggcgactg
     2881 ctctatccag aggaaaacaa acccaaagca ctcattacta cttgcttcac tggaatcggt
     2941 acggcggtcc acttaagtga actgctagaa aaaagcttac ctacggcttg tcagttgaaa
     3001 atcatcccat atgaatatca gcaattaaaa gataagaaaa acagtgaccc attattttcc
     3061 atatacgaag tcgtaggaat gattggcacc acagatcctg atatcaccaa tatcgactat
     3121 ctttcattag aagacctcat ctcgggggaa aagatgtctg tactagctga atggttggac
     3181 actactatga atgcttcaga gaaggaaata ttcaatcagc gcatcattcg taatttttca
     3241 ttggaaaaag tactggattc cgtgactatt ctagatacag agaaggtgat gggagaaatc
     3301 gatcatttta tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa tcccaaaaaa
     3361 ttagcgttgt atgtccatgt gagctgtctc attgaacgat tgatccgaca aatgccaatc
     3421 gaaacctatc aaggattaga gaaatggaag cagtgtcaaa aagaagggtt atctgctatt
     3481 aaatctgcgt ttagtgtcat tgaagagcat tatagtgtca tgatccccaa ttctgagatt
     3541 gcctatatct atgatatttt gagtgaagat acagagtttc tctcgataga agaagaattt
     3601 tgaacataag caagttgttg ccactaattt tggcatgcaa cttgcttttt tagtatgtgt
     3661 aagtgaaaag aggagatgaa aaagtggata gagaaattat cttagcttcc catggaaaat
     3721 ttgcttcagg gatattagat tcattagagc tgatctatgg gaaaaatcat tctattactg
     3781 tattcgattg ctatactgat gagaattttg atttagcgga aacagtaagg cagcttttta
     3841 tgaggtacaa agataaagaa atcattgttc tgacggatct ttttggcgga agtgtcaaca
     3901 acgaattctt acaatatatc aatcgggatc atgtctatct cgttgccgga ttgaatttgc
     3961 cactattgat tgagctagta tctcggttgg acgctgaagt agagacaact gttttgatcc
     4021 agcaggtgct aactgattca aaaaaaatga tccagttttg taacgagcgg ctcaatcaga
     4081 aaatagaaga agacgaattt taggaggaag aaaaatgatt gtattgacaa gagtcgacca
     4141 tcggttatta cacggacaag tagctttttc atggacacaa acaataggcg cagactgtat
     4201 tttgattgct aatgatgatg tacctacgaa tgagatacga aagacaacga tcaaattagc
     4261 caagccacaa ggagtcaaac tagtgatcaa atcaattgat gactcgattg ccgcgttgaa
     4321 aagcggggtg acagataaat ataagttatt catcgtagtg gaatcgatcg aggatgcata
     4381 taagctggca gaagcgtatc cagaaatcaa aaccatcaac ttaggaggga tcaaggcaaa
     4441 acctggtact cgcagcatcg gaaaagcggt aaatatttta ccagaagagg aggaacgctt
     4501 gaaacgatta attgacaaac aaatagaagt agaaatccgt caagtaccag cagataaaaa
     4561 agtggatgta agagaagttt tgtaagaaag gaagaacctc aatgattgta caagcagtct
     4621 tattaggaat tgttgcattt gtcgcacaat cagaatatgc actagggaca tcgttgcttt
     4681 ctcgtccgat tgtgacgggg ctgtttacag gaatcgtgtt aggtgatgta aaaactggga
     4741 tcattatggg cgcaacttta gagttagcat ttatcggatc gttctcagta ggtgcttcga
     4801 ttccacctga tgttgtgacc ggtgggattt tagggacggc ttttgctatt acagctggag
     4861 ccggtacaga aaccgcgctt ttactaggtt tgccgattgc tacgttgaca ttgatattga
     4921 aaaatattta cttagggctt ttgattccaa ttatgaacca taaagcagac aattatgcag
     4981 aagaaggaaa ttataaagga gtcgagcata tgcacttgct tgccggtatc ggtctttcct
     5041 tgatgcttgg tgttgtggta atggtttctt atatggtggg aagcaatacg atcggaaatc
     5101 tattaaatat gattcctgac ttcgttcaaa aggggttgtc agtagctaca gggttgattc
     5161 cagctcttgg ttttgcgatg cttgctcgtt tgctgatcaa caaacaagtt gcaccatatt
     5221 ttttcttagg ttttgctata gcttcttact tagagattcc tgtgaccgga gtagctattt
     5281 ttggtgcgat tttagcagtt gtggtagtca atattatgaa tgtccgtcaa ctaccacttc
     5341 aaacaactgc aggggaggta gaagaagatg aagaattctg agatcaaaaa cagcaaaata
     5401 acgaaaaaag agctaaatca cgtattttgg cgttcttttc aaatggagtt ttcttggaat
     5461 tatgaacgtc agatgaatat ggcttatgtt tatgcaatga ttccgatttt gaaaaagtta
     5521 tatcagtcga aagaagaaat ggcagcagct ttgaaacggc atctagagtt ttttaacacg
     5581 accccacata tcgttacact gattttagga atcacggctg caatggagga agaaaataag
     5641 gatgatcctg aatttgatgt gactgcgatc gatagtatca aaacatcttt gatgggacct
     5701 cttgcagggt taggagattc tttcttttgg ggaactttgc gattgattgc cacaggtgtg
     5761 ggtacctctt tagccatgca aggaaatatt ttaggcccta tcttgttcat tttgattttc
     5821 aatattccgc acattctgtt tcgctatcta gcaacaggtt ggggatatcg cttaggaacg
     5881 ggttttttga aaaagatcca ggcaaacggt atgatggaaa gtttgacatt aggcgcgtct
     5941 attattggac tgatggtcgt tggagcaatg actgccacga tgatcaccat caatattcct
     6001 ttaaaaatcg gttcaggaga aaatgctgtg actgttcaaa gtatttttga tgatatcgtt
     6061 ccaaatatct tatgtcttgg agcatttggc atagtgtttt atctattgaa gaaagaagtg
     6121 aaaccattaa cgatcttgat cggactagct ttctttggaa tttttggttc gtggataggg
     6181 attttttaaa aaaggagcag agaaaatgtt tacgatgcaa gattatatct atgaagaaaa
     6241 ggaagtacta agtacgatct tgaaaaagaa tgatttttcg acaagagaac atatgaaaaa
     6301 aacagtgaat ttactgatct tagcaacggg ttcatcttat aacgcttgtt tagcagcgaa
     6361 gccagcattg gaatcttatg gtgatcttac tgtcgatatt caagaacctt tttatttttt
     6421 caattatgga aaactttctc cttcaatcga tactgtgatt gctgtgtcac aaagcgggaa
     6481 aagtgcttct actgttgaag cagtaaaaat gatacaaaaa caagggttac ctgttgttgc
     6541 tatcacaaat gacgtgcaaa gtccacttgc tttagaagct gcacaaatta ttgatttagg
     6601 tgccggggta gaatcagtag gcttcgttac aaaaggctat tctgcgactg ttttacagtt
     6661 actactctta gggataggaa ttggaataag taaaaataaa atcagcaaaa aaatagaaca
     6721 agactacatg caacagttgc gcaaaataat caaccattta ccagcgatta ttcaaaaaac
     6781 ggaagaattt tttgatggat atcaaagttt atttcgttta gcacagcgtt tcgtcaccat
     6841 tggatatggt ccaaactggg gaacagctaa agaagcagag acgaaattga ctgaaacaat
     6901 ccgcgttccg tcacaaggat ttgagttaga agcttatatg cacggcccat acctggaggc
     6961 agatgcttcg catttgctat tctttattga aggggaaagt gtaaataagg aacgttcgca
     7021 aaagttgcaa agatatatga gtcggtacgt tggagagact ctcaccatta ccacaaaaaa
     7081 agcaagaaac gagaaaacac ttggattagc aatagaatgt gatgaatatc tgtctgtatt
     7141 agcgcttgtc gttccgtttc aactattcgc ttataaaacc gcagtagcta aaggtattga
     7201 cttgaacaaa aagatttttg aagactttga tacagtcttg aaaagtaaat tataagaggt
     7261 ggaaatcatg ctgaaattca atgaagaaga acaaatcaaa gcaataaaag gcgcactagc
     7321 tttacgtccg caagtagaag aaatcatcga taaactttat gcggaaaaat ttgacgctgt
     7381 ctattatcta ggaatcggtg gaacgtatgc ttcctcgatg caagctgtca cttatatgaa
     7441 tgggaaaagc aacttgcctg tctttgtaca gcatgctgca gaatattaca ctactggaaa
     7501 caaaagatta acaaaagatt ctatcgttat tttgtcctct gttaccggta caactcaaga
     7561 agtagtgaaa gcagtagaac aaatcaagaa agtaggagcg acactgattg gatttatcga
     7621 taaagcgaac agtaagttgt ctcagctatg cgattttgtg gtcacttatc cagctcctgg
     7681 aacagaacaa atcaaatttt ttatggcagc agatcgattg atgtatttga atggagaatt
     7741 cgaagcttat tcagaatact atgaacagct cgaacgttat ttaccaacag ggttagtgga
     7801 agcagagaaa aaagcagatg catttggctt atcatttgca gaaaaacatc gacgtgatag
     7861 tatgcattat tttattggag cagggaatca atggggagct gtttattcat atgccatgtg
     7921 ttactgggaa gaacaaagct ggcttccatc gaaatctatc catgctgccg agtttcttca
     7981 cgggacgtta gagatcgtag aagaaacaac cccagtcaca ttatttttag gcgaagatga
     8041 acagagagtg ttatctgaac gtgtggctaa acttttgcca aaaatttgca gcaattatac
     8101 attgatagac acaaaagact atccagtaga aggtatcagt gaaaaatatc gcggacgtgt
     8161 tttgtctttc ttattgatgc atgtagtgac acaacgcatc gatgctcatg tggagcagtt
     8221 gaactgccat ccattagaga tacgccgcta ctatcgtcag tttgattatt aggatagact
     8281 ggataaaaag aatataaaaa ataaagagag cagcgataac tgctcccttt atttttttaa
     8341 aatattggca tcaattacgt tataaaacac attttttgca ttatctacag ttcaacttat
     8401 aatctgttta atgggacatg tgggtgccaa ccattttttg ttatgcaata gtactattta
     8461 gttgttgcat ggatccgtag taaaagtcca agtacaacga gttgcgccag tttacatagc
     8521 gttttgtatc cagaatgttg actaaaacaa aagttcctat gtcaaccgtc aacagagaca
     8581 atctgtccgt tgcaggacct gacagattta gaaatgttca atctgtctct tatgtatttt
     8641 aaatcaatac tcactaggag aagtttccta acctcctaat agtaatgctg gtagtaacat
     8701 tgtcaaaact gcttcctctt aggaacaagc aaagcataac gagtgtaaaa ataaaatgta
     8761 gactattgta acatatcatt gattatttca agtaaaaacg ataaaagaag taaacaaaca
     8821 cttaaaaaca atggaaatta gtcccaactt taagaaatgt atactataat ggaaattatc
     8881 aattatttat ttggaggaat atccatgaaa caaaaaatcg cagttcttgg tcctggatct
     8941 tggggcactg ctttagcaca aacattggca gaaaacgggc atgatgtccg tatttgggga
     9001 aatgtacctg aacagattga tgaaatcaat aagtatcata caaatcagca ttttttacct
     9061 gatttgacga tcccagaatc gattatcgga tataaagaat tggcagaagc agtggataac
     9121 gcagatgcta ttttatttgt tgttccaaca aaagcgattc gttcagtcgc acaggaactt
     9181 attggtaaaa tgaatactaa gccaatcatt atacatgcaa gtaaaggact agaacaagat
     9241 acgcataaac gaatctcaga agttttggct gaagaaattc cagaagaaaa aagacaggca
     9301 attgttgtat tatctggtcc tagccatgct gaggaagtgg cagtacatga tatcacaaca
     9361 attacagctg caagtattga tcaaaaagca gcagcctatg tacaaaaact gttcatgaat
     9421 gattatttca ggatttatac aaataatgat gtgattggcg tagaaactgg tgcagcactg
     9481 aaaaatatca ttgcaattgg tgccggcgct atccatggat taggattcgg agatgatgca
     9541 aaagcagcaa tcatgactcg aggattggca gaaatcagcc gtcttggtgt cgcgatgggt
     9601 gccaatcctc tgacatttat cggtttgagt ggtgtggggg acttgatcgt tacttgtaca
     9661 agtgttcatt ctcgcaattg gcgtgccggt aacttgttag gacagggaca taagctggaa
     9721 gaagttctag aaaacatggg aatggtcgtt gagggtgtgg caacgacaaa agcagcagta
     9781 gagctagctc aacagctagg tgttgagatg ccaattactc aaacaatcta taatgttttg
     9841 tataatggag aagatatcaa aaaagcagca aaagaaatca tgttacgtga cggaaaaatg
     9901 gagaatgaat tttctcttcg ttaaatagat gatcaatagt taatcgt
//
LOCUS       DAZUMM010000087         9925 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000087 DAZUMM010000000
VERSION     DAZUMM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.090"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..96
                     /locus_tag="ACG8E6_002244"
     CDS             <1..96
                     /locus_tag="ACG8E6_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 32"
                     /protein_id="HFS5062629.1"
                     /translation="LVNIRGKQTMRTCYLMSWNASSRTAKYYYYI"
     gene            902..1075
                     /locus_tag="ACG8E6_002245"
     CDS             902..1075
                     /locus_tag="ACG8E6_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062630.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYN
                     SPNEELFVKQTDK"
     gene            1533..2633
                     /locus_tag="ACG8E6_002246"
     CDS             1533..2633
                     /locus_tag="ACG8E6_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFS5062631.1"
                     /translation="MIGIFSIGITNTYASENSNFNINSQPIEEQAINFAKTDFEHSIT
                     SILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNTIKYIYTVKKNTNG
                     EYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIKLLWKSPLSTEKNGVS
                     SFTEKNDLISVNIKNKIKNFSKKQNFPAIKRVKSVSSGDNFIMINWRIAETQTSFPWC
                     AAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIESKSLNQDQIIKYANYCGVYPK
                     KVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQGGYQTYYVWNPW
                     YNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            2606..3049
                     /locus_tag="ACG8E6_002247"
     CDS             2606..3049
                     /locus_tag="ACG8E6_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062632.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3351..3731
                     /locus_tag="ACG8E6_002248"
     CDS             3351..3731
                     /locus_tag="ACG8E6_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS5062633.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            3713..4627
                     /locus_tag="ACG8E6_002249"
     CDS             3713..4627
                     /locus_tag="ACG8E6_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS5062634.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            4587..4865
                     /locus_tag="ACG8E6_002250"
     CDS             4587..4865
                     /locus_tag="ACG8E6_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062635.1"
                     /translation="MKEEQTTNKNYRSKSLKDIENEIKKQRAFLVPYTQERIQTTIDL
                     QVKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
     gene            4867..5556
                     /locus_tag="ACG8E6_002251"
     CDS             4867..5556
                     /locus_tag="ACG8E6_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062636.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQVLAQKIEKFTA
                     CYEDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEQQKEYQEKYEQVKKDVQYVKDTKV
                     EEACSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(5676..5762)
                     /locus_tag="ACG8E6_002252"
     CDS             complement(5676..5762)
                     /locus_tag="ACG8E6_002252"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS5062637.1"
                     /translation="MFELFICPLLVGVCISVFSYWLNNRNKK"
     gene            6408..7340
                     /locus_tag="ACG8E6_002253"
     CDS             6408..7340
                     /locus_tag="ACG8E6_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5062638.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIH
                     GEVDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEAD
                     FKKQQQTEKEEAEKQSISNELKQDLLENLQNLFD"
     gene            7357..7827
                     /locus_tag="ACG8E6_002254"
     CDS             7357..7827
                     /locus_tag="ACG8E6_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS5062639.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTL
                     VVSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLD
                     QQQQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
     gene            7911..8363
                     /locus_tag="ACG8E6_002255"
     CDS             7911..8363
                     /locus_tag="ACG8E6_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5062640.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDT
                     ENLIQFAKLFDLSLDNILLGEEKIMVKNHTKTLILLFHFVIFLSLVFTLIQSGFFYFS
                     HSIATWLLIFFLSLLLIINTLIIIFNKHRNLFLHSIFPIVLLIFLYFI"
     gene            8490..9671
                     /locus_tag="ACG8E6_002256"
     CDS             8490..9671
                     /locus_tag="ACG8E6_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS5062641.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKI
                     TKELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVL
                     TGAKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIAT
                     LPIGILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISD
                     TGRDFLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNI
                     HVPVRESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVA
                     MIFFHVFENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQ
                     K"
     gene            9761..>9925
                     /locus_tag="ACG8E6_002257"
     CDS             9761..>9925
                     /locus_tag="ACG8E6_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 32"
                     /protein_id="HFS5062642.1"
                     /translation="MKKTKLLVASLCLFSSLLAFTPSVSFSQNGGVVEAAAQRGYIYK
                     KYPKGAKVPNK"
BASE COUNT         3683 a         1575 c         1635 g         3032 t
ORIGIN      
        1 ttagtaaata ttagaggtaa acaaactatg cgtacttgtt accttatgag ttggaatgct
       61 agtagtagaa cagccaaata ttattactac atttagtaaa aacaaaacat tctttctata
      121 caataaaata gcgtatagag aggatgtttt taatttgaaa aggagtggtt atctttatga
      181 aaaatagatc atttgctata ttagtattat ccgtaatttt attagatata ttgttaataa
      241 cattagcttt aaaaacttta aatttagtaa caagttttgt cattatacca gtaattatag
      301 gaatgtctat ttatttcatc aaaaaaaata aacaattttt ttgaatgaaa ttacgggcga
      361 aaataatcac tcaaaattat taataaaaac gaatcaacta ctttttctga agaaacatac
      421 aaccgtaaac aacaattgtt gtttacggtt gtaaattatc aaactatatg gtgacttgtt
      481 gcttaaaata ggttgaaatt tcgtttattt tttttgagaa attttaccca cgtactaccc
      541 atgtacctat ccacgtatta cccatgtacc cccccatgac aaatgggcgg aggggcttgc
      601 ggcgcttggt attaaaggca ttatccgcct cccaggtttc tgaccatttt ctctcataag
      661 tggggttttt accgcgttga gagaaaatgg tcagaaacct aaacgatcgg caaagaaaac
      721 ataccaagcg ccacaagcaa aaaaagttag agtaacttcc agaaaagaaa aatttttaga
      781 acaacttttg aaaatttgat tttgatttat atccgttcaa tttaaacatt tgataaactg
      841 aaggctagta tttaaaatgt gtatacaagt ggtagcgtta aaaaaagtgg aggtataacc
      901 tatgttaaaa aaaactgtgt tggtactggg ttctatttct ctaggaatat taggttttag
      961 ccccatgcta ttatccgtaa ttggaaaaaa caattccaac agagaactta aattaaattc
     1021 aaattattac aacagtccta acgaagaatt gtttgtaaaa caaacggata aataataaaa
     1081 aagactgctc tttcgagcag ccagttaaaa aataatgagt gtttattctt gaaaaagcac
     1141 gcgcttatga agcgctacca aaaatttacc atattttaca aaaaataaca agtcttaaaa
     1201 aaatgacaga taaaaatgca caaagaatta gatatagata accgcgaaaa atcaagcttt
     1261 agaaagttgc ttttagctag accaattaaa aatcacaaag tgagcgtctc tatccattgg
     1321 tagaggggct acttggcata ctgtacatta tgttaactaa cgctataaat aacgattatg
     1381 ttcccaaaac aatattttat gaactataac aaaaatttgt tatcgcatat tgtatatttt
     1441 aattataggt gtataccact taaatttaac acaaaggcgt gattattttg aaacccaaaa
     1501 agaaaattta tctaggtctc ctcttttcct tgatgattgg aatttttagt ataggaatta
     1561 caaataccta tgcttcagaa aattctaatt tcaatatcaa ctcccaacca attgaagaac
     1621 aagcaattaa ttttgcgaaa actgattttg aacactccat aacttcaatt cttttaatgg
     1681 aaaataacac cgaaactgat attaactata ctcttggttc gccatttaaa atgctcgatg
     1741 gagaaaatac ggcaaatagt tctattttct ttccagtatt ttcagacgct gataatacga
     1801 ttaaatatat ttataccgtc aaaaaaaata caaatggaga atattcagga agtatttcac
     1861 aatttttagc aaaagaactt caaaatctag tagatagtaa tataacagat acaaccttat
     1921 tttttcaaga taatagcatt tattattccg ataatggaca aattaaacta ctttggaaat
     1981 cacctttaag tacagaaaaa aatggtgttt cttcctttac agaaaaaaat gatcttatat
     2041 ccgtaaatat aaaaaataaa attaaaaatt tctctaaaaa acaaaatttt cctgcgatta
     2101 aacgtgtaaa aagtgtctcc tccggagata actttattat gattaattgg cgtatagcag
     2161 aaacccaaac aagttttccg tggtgtgctg cttacgtaga agctgctatc ttatgtaaca
     2221 aaacagatgt acatgctact tcagctagag ccattatgca ttatacctat cctaatttaa
     2281 acaacagtca aattgaatca aaaagtctta atcaagatca aataataaaa tatgcaaatt
     2341 actgtggtgt ttatcctaaa aaagtagcac gaatactaaa cctttcagag gtccaaacac
     2401 aactaagaaa aggaaatgcc atttatatgg gaactgctgg ttatggaaat tatagtaaca
     2461 gccgtcatgc acttgcatta tttgggtggg tcaatcaagg tggttatcaa acttactatg
     2521 tgtggaatcc ttggtataac tacccaactg taataagtgc aaatgcagga ccagttacaa
     2581 ttcctgtacc tggaggtggt tttacttggg acaataccat tacaaattgg taaataaacc
     2641 cattgcttta gttatgggtc tagtgttagt tcttttctta actagttgtt caaaaaaaca
     2701 agaaatacaa aagactgatc tgattccttt tctaggaact tggcaaacta aagatcattc
     2761 agaacgtcta gtaatcacta atttaaatac ttcaaaaaat atagaacaat ataccatctt
     2821 agtaaaagag aacaatcata aaattcaaat cattgataaa aatttaaata taaatgaaca
     2881 agttttcatt tacaaagcaa caaataattt actaacactg cgctctaata aaaaacaatt
     2941 agattacact tttagtttgg attctccaaa aaagctcgta tttattttga caacgaatga
     3001 tgggacagaa gggacagcta aaccaattga attttttaaa gctgattgat gaatagttgt
     3061 ttgatattac aaaacaataa tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg
     3121 gctagcattt gctagccttt tttgtattgt ggagcgtggg ttttgagggg gattcaaggg
     3181 ggagcttgcg cccctttgaa aagcggttat ttgcaccaaa tacgtaggta tttggctagc
     3241 aaatatagag cttgccaaac ccttttagtg agctttactc attgtacctt tttaggtaca
     3301 atggtattgc gaacgatacg tccgtaggac gtactgaaag ggtgggactt atgagcgaac
     3361 aaaatcaaaa tttggctagc gatcattcta aaaaatatac ctatcgatcc gagccaaaac
     3421 aaatcagttt tcgagtgagc gaatctgaat ttgcaaagct gaagcagtca gctgaagctt
     3481 tgcaaatgag tgtgccggct ttcgtaaaag ccaaggcaca aaacgcgcgt ctggtgacac
     3541 cgaaagtggc gccagacatt gcccaggcaa tcgcgcgcga tttagccaaa gctggaggga
     3601 atatcaatca aattgctaag tggtgtaata cacaccaaca cgacgttgct cctggagacg
     3661 cccaacgctt atccgaaaac ctaaaaatca tgcaaaagga gctacaaaag atatggcaac
     3721 agttaaagta agccgaacca cttcttgtag tcgtgcaatt aattacgctg aaccacgagc
     3781 aaccattaaa acaggaataa actgtgacgt cgattatgcc aaaagtgaaa tgaaacaaat
     3841 tcgcatgctg tatggaaaag acgaccgtgt acaggcacac ttattgattc aatcttttcg
     3901 accaggagaa attaccgctg aaaaagctaa tcaactaggg gcagagtatg caaaaaaaat
     3961 agcccccgat caccaaatag ctatttacac acatacagac aaagaccata ttcataatca
     4021 cattgtcatt aactccgtta atctggagac tggaaaaaaa tttcaagctc atggtcaagc
     4081 atttctaaac aaatgttatg acctaaatga cgagatttgt cttgcacata acttaccaat
     4141 cactgagcga ggaaagaaac cagaaaaacg aaccatgtct gaaataaaat taaaagaaaa
     4201 aaatgaacct gtttggaaag acgaaattcg gattgcgatc gatcaaacaa tgaaaaacaa
     4261 aaaaaccaga acctatgacc aattttgtaa ttccctcaaa acattgggga ttcattgctt
     4321 caatcgcggg aaaaatttca cttacgaatt gatccacaaa aagaaaaaag tacgatccaa
     4381 cagactagga aaggactacg aaaaggagac gattttacat gagctggaca gacgagaacg
     4441 aacctataac caacagcgaa ccaacgagct acgaagagct cgagctagca ttcgcgaaac
     4501 tgcagaagaa gtacgaagag aacaacgaac tcttacgcct gaacaaccgg aaattagacc
     4561 ggctcgaaga aaccatgaac aacacgttga aagaggaaca gaccaccaac aagaattatc
     4621 gctctaaatc gttaaaagac atagaaaacg aaataaagaa acaacgtgct tttctcgttc
     4681 cttatacgca agaaaggata caaactacga ttgatttaca agttaaaaaa ctccatcaag
     4741 agctattaga ccagcgaaat aaggaacaga aaaaggtgct tattcaacga tcattggatt
     4801 ttcttgcccc cttatgttgc ttagtcctga cactgttcgc attttggtta gtatggggag
     4861 gctaaaatgg cattttattt accctatcta ctcatttttg ttagtatttc aggaagtatt
     4921 tggctcatct ataaaatttt tcaaacaaga tattcattaa aaggctctaa aattcgattt
     4981 aagcgatttt tctttcttgg ctgtatattt tccttgataa tcgtttcaag cggcttattc
     5041 ggcgttttag agggaaataa gcgcgtttct agaagtattt tattaggaaa tgtcactcaa
     5101 aagtatgaat cagcgcgtga taaaaagaaa aaagagcaag tattagcaca aaaaatagaa
     5161 aaatttaccg cttgttatga ggacatgaat gatatttttg tgaagcaaga aaagcgtttg
     5221 acggataaaa atatggaaac actcactcga ctttatcgga atttgccaga agaacagcaa
     5281 aaagagtacc aagaaaaata tgaacaagta aaaaaagacg tgcaatatgt gaaagatacg
     5341 aaagtggaag aagcttgtag tgatttattt ggagatacaa ttcctttttc aactagcgaa
     5401 caagaacgaa aagaacgaca acaatcagtc acatatgaac gctatgaaat cttgcttcaa
     5461 caagcaatta acatacaaaa tctgactaaa aaagaaacag ctttaaatta tttaaaaagc
     5521 gtcaaggaat ggttagatca gcaacagcaa aattgaattt gattgaaatt catgttaaac
     5581 taaacatgca aatgttcaaa cattagatac acgaaagccc cagagaggtc ggattctctg
     5641 gggctttttt taggttgatg tcgcctttac tgattttact tcttgtttcg gttattcaac
     5701 caataactga atacagagat acaaactcct acaagaaggg ggcaaataaa cagttcaaac
     5761 atcagataca cctccttcca gtcgcaagac tgtcggatag gcgacaagtt attataccct
     5821 acaaaattaa atctgtgtgg caattttaag tatctcttat ttccgggttg ttagggcttg
     5881 ccctgaccgc ctgtagggcg ctttaaatgc cagataaagg tttttatact aagactgtct
     5941 taaaacggct tagacaagct cttagagcct caaccctcct ttttgaacaa tggttttagc
     6001 aaaggagctg gaggcgactg cgcaggtata atgtgagagc gtagcgatca cacctttttg
     6061 gcgaccgtag ggagcgaggt tttgaattag cagtttagaa aagttcgcga acctgtttaa
     6121 agaaaaaata attgtttttt taggtgggaa attttgtctc cctcttttat aatctttttt
     6181 taaacctctt tttaaacctc tttaccgtcg gctgtgtcct tactccccca agggatacaa
     6241 aattttatag gcatgaaatt atctgtttat aggcatgaaa ttatctgttt ataggcatga
     6301 aattatctgt ttataggcat gaaattatct gttttaggta ttgtttttag acataatatg
     6361 tcctaaaatg atgtcaataa aatcatttag actggagtaa caactaaatg gcaaatgaaa
     6421 tcgtaaagta tgaaccagaa ttaaatacca ttcctttaag aaaatttacc ccaatagaaa
     6481 tgaacttgtt tttttctatt gtctctagaa tgcgcgataa aggtaatcaa aaaatacgct
     6541 tttatttcag ccaacttaaa gatttaagtg cgtataagca gactgcagga gaagtcttta
     6601 taaaagattt ggcaagaaca tacgatcatt tgatggattt acgtttcgga agaaaaagca
     6661 atggtggatt aacaatagaa cgatttacaa tgtttactga ttttgaaatt catggagaag
     6721 tcgataaccc ttatgtagat ataaaagtcc acgaaaaagc tttgccttta cttaataatc
     6781 tagaaagctg ggtacgtttc tctttaacag aatttcgaga tttaaatagc agttatgcta
     6841 aaacaatgtt tagattgcta aaacaatacc gaactcaagg atgggctgaa ttttctaagg
     6901 aagatttttt agagctatta gatattccta aaagctatag acaaacaaat ataaatcagt
     6961 ttgtactcaa accaataaaa gaagaactaa ctccactatt taaaggatta actattcgta
     7021 aaaaatatgg taaaggtcga ggtaaacctg ttattggtta tcgttttact tggaaagctg
     7081 aaatcaacca tgctgacgat ttttcaaaag ggaagcaaga agatttacgt ataaaattat
     7141 ttaatattga acacaatgga gaattaacac aaaaagaaaa atggcgtgct aaagatcgaa
     7201 tactaaatct tccgttaggt actcatgaag ctgattttaa aaaacaacaa caaaccgaaa
     7261 aagaagaagc ggaaaaacaa tcaatctcaa atgagcttaa acaagattta ttagaaaact
     7321 tacaaaactt attcgattga agcataagga gtatttatga tagataaaac gatcaaagaa
     7381 ctcgcggaag aatttcatgt ttcaaaacaa gcaattagga aaaggctatc agctgatttt
     7441 agagaaaact atgtgcaaac tgttactagg aacggagtga aaaccttagt tgtcagctac
     7501 aaaggatatt tgcttttaaa acaacatttt ttaggtagta acaccactgg taaccaagta
     7561 gaaaccgttg ctagtaacga tgaaaactcg attattgttt ttttacatga acagctccaa
     7621 gaaaaagata ctcaaataaa acaaatgcaa aagttactcg accagcaaca acaactcacc
     7681 ttgcaggcaa atcaacagat cgaaaaactg caagaacagt tacaattagc ttatacggaa
     7741 gaacctgaag aagaggaatt aacttctgtc aataaagaaa acaacaatgc aataacaaca
     7801 gaaaaaaagt ggtggaaatt ttggtaacaa ttaccaactt tccataaaga agaaatcaat
     7861 ttctatagac tctatctttc taagaataaa aaatatgttt aggtgatcaa atgactataa
     7921 gtaaagtaat caaacaaaaa agaatagaaa aacaactaac tcaagaagat ttagctgaaa
     7981 tgcttttagt atctaaaaaa accatttcta attgggaaaa tggtcgaaca ataccagata
     8041 cagaaaattt aattcagttt gctaagttat ttgatttatc actagacaat attttgttag
     8101 gagaagaaaa aattatggta aaaaatcata caaaaacact aatactatta tttcattttg
     8161 taattttttt aagtttagtt ttcaccttaa ttcagtccgg attcttttat tttagccatt
     8221 caattgccac ttggttatta atattttttc taagtttact attaataatt aatactttaa
     8281 ttattatctt taataaacat agaaacttat ttcttcacag catatttcca attgttttat
     8341 taatttttct atactttata taaaaagata gaactattta gtaagataaa aaagtagcta
     8401 attgtaattt tcgtctaaaa ctcaaaacta gtaaaaataa ctttattgtg ttatttttat
     8461 ttataaatta tgaaaaagga cctttcgaaa tgaaaaataa tagaatctac aaaaatcagt
     8521 tgaattatgg gcttttattg ccagtttttc tgctatgtat cattggatta ctttctttat
     8581 atgttgcttt atatcacgac tcttacacta attcaaaaat cacaaaagaa ttaattaaac
     8641 aagtattgtg gtatagtatt gggttcactt cagcaataac tatttatttt tttaatccta
     8701 aaattttgtg gaaattaact cctatatttt acggattatg catcataatg ttattacttc
     8761 tcttgaaatt ctataatcca caattagcag tcttaactgg agcaaaaaat tggttttata
     8821 ttgctggttt ttcgtttcaa cctactgagt tagtcaaaat aggatacacc cttatgctag
     8881 ctaaaataac cgtcaattat cagcaaaaat ctattactag taatacgaaa gatgattgga
     8941 tttatttact taaaactttt attgctactc ttccaatagg aatattatta ttttttcaga
     9001 acgatctagg aacaatgtta gtcttcattg ttatttatat ttttattttg tttacgtcta
     9061 acataaatat aaaaataatt gccccaataa tcgccattgg tgttgtttta tccttaatat
     9121 ttttatattt ggttatatca gatacaggaa gagatttctt attaaaaata ggatttcacg
     9181 actatcagtt taaaagaata gactcatggt tgaatccatt tttagatcca aatggatcta
     9241 gctatcagtt agcaaatagt ttaattgcta ttggctctgg cggactttta ggtactggtt
     9301 ttaatgtatc aaacattcac gttcctgtta gagaatccga tatgatattt tctgtaattg
     9361 gagaaaattt tggctttata ggaagttgtc ttgtaatatt cctatacttt tatttaattt
     9421 atcaaatgta taaaacatgt tttaaaagca ataacttatt tttcacctat atcggtatag
     9481 gcattgttgc aatgattttt tttcatgttt ttgaaaatat aggtgcaagt atcggattac
     9541 tacctctaac tggtattcca ctcccctata ttagtcaagg tggttctgcc attcttagta
     9601 attttttggg gttgggattt atgttgtgtt gtagtagata tactaacgaa aaaaaaggaa
     9661 cacaaaaatg aattcaaaat aacactttat gaatagaatg tctatttcaa taaaaattat
     9721 gataccttaa agttaatcat taaaaaagga gtgtcataat ttgaaaaaaa caaaattatt
     9781 ggtagctagt ttgtgtttgt ttagcagttt attagctttc accccttctg tttcattttc
     9841 tcaaaatggt ggagtggttg aagctgctgc tcaaagagga tatatctaca aaaaatatcc
     9901 aaaaggtgct aaagtgccaa ataaa
//
LOCUS       DAZUMM010000088         9706 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000088 DAZUMM010000000
VERSION     DAZUMM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.091"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACG8E6_002258"
     CDS             168..362
                     /locus_tag="ACG8E6_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062643.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="ACG8E6_002259"
     CDS             631..930
                     /locus_tag="ACG8E6_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062644.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="ACG8E6_002260"
     CDS             complement(1027..1461)
                     /locus_tag="ACG8E6_002260"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5062645.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="ACG8E6_002261"
     CDS             complement(1693..2403)
                     /locus_tag="ACG8E6_002261"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS5062646.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="ACG8E6_002262"
     CDS             complement(2393..3253)
                     /locus_tag="ACG8E6_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFS5062647.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="ACG8E6_002263"
     CDS             complement(3258..3896)
                     /locus_tag="ACG8E6_002263"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFS5062648.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="ACG8E6_002264"
     CDS             complement(3911..4210)
                     /locus_tag="ACG8E6_002264"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5062649.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="ACG8E6_002265"
     CDS             complement(4253..5737)
                     /locus_tag="ACG8E6_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS5062650.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="ACG8E6_002266"
     CDS             complement(5757..6218)
                     /locus_tag="ACG8E6_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5062651.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8E6_002267"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8E6_002267"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFS5062652.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="ACG8E6_002268"
     CDS             7521..8291
                     /locus_tag="ACG8E6_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5062653.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="ACG8E6_002269"
     CDS             complement(8564..9391)
                     /locus_tag="ACG8E6_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5062654.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         3035 a         1968 c         1509 g         3194 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgcccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatacttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttat
//
LOCUS       DAZUMM010000089         9689 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000089 DAZUMM010000000
VERSION     DAZUMM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.092"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..1183
                     /locus_tag="ACG8E6_002270"
     CDS             185..1183
                     /locus_tag="ACG8E6_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5062655.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="ACG8E6_002271"
     CDS             1295..1804
                     /locus_tag="ACG8E6_002271"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFS5062656.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="ACG8E6_002272"
     CDS             complement(1834..2493)
                     /locus_tag="ACG8E6_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFS5062657.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="ACG8E6_002273"
     CDS             complement(2495..3259)
                     /locus_tag="ACG8E6_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFS5062658.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="ACG8E6_002274"
     CDS             complement(3267..3944)
                     /locus_tag="ACG8E6_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFS5062659.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="ACG8E6_002275"
     CDS             complement(3981..5366)
                     /locus_tag="ACG8E6_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFS5062660.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="ACG8E6_002276"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8E6_002276"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACG8E6_002277"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACG8E6_002277"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFS5062661.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="ACG8E6_002278"
     CDS             6305..7162
                     /locus_tag="ACG8E6_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5062662.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="ACG8E6_002279"
     CDS             complement(7243..7578)
                     /locus_tag="ACG8E6_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062663.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="ACG8E6_002280"
     CDS             complement(7645..9015)
                     /locus_tag="ACG8E6_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFS5062664.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="ACG8E6_002281"
     CDS             complement(9093..9629)
                     /locus_tag="ACG8E6_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5062665.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2846 a         1998 c         1619 g         3226 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag taaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaata
//
LOCUS       DAZUMM010000090         9306 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000090 DAZUMM010000000
VERSION     DAZUMM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.093"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..705)
                     /locus_tag="ACG8E6_002282"
     CDS             complement(37..705)
                     /locus_tag="ACG8E6_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5062666.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            839..1126
                     /locus_tag="ACG8E6_002283"
     CDS             839..1126
                     /locus_tag="ACG8E6_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062667.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1542..3080
                     /locus_tag="ACG8E6_002284"
     CDS             1542..3080
                     /locus_tag="ACG8E6_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFS5062668.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3077..4042
                     /locus_tag="ACG8E6_002285"
     CDS             3077..4042
                     /locus_tag="ACG8E6_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5062669.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4029..5351
                     /locus_tag="ACG8E6_002286"
     CDS             4029..5351
                     /locus_tag="ACG8E6_002286"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5062670.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5414..5518
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5592..6335
                     /locus_tag="ACG8E6_002287"
     CDS             5592..6335
                     /locus_tag="ACG8E6_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5062671.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAV
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6350..7342
                     /locus_tag="ACG8E6_002288"
     CDS             6350..7342
                     /locus_tag="ACG8E6_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5062672.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7354..8091
                     /locus_tag="ACG8E6_002289"
     CDS             7354..8091
                     /locus_tag="ACG8E6_002289"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5062673.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8081..8803
                     /locus_tag="ACG8E6_002290"
     CDS             8081..8803
                     /locus_tag="ACG8E6_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS5062674.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8887..>9306)
                     /locus_tag="ACG8E6_002291"
     CDS             complement(8887..>9306)
                     /locus_tag="ACG8E6_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS5062675.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3048 a         1603 c         1966 g         2689 t
ORIGIN      
        1 tttctgtaaa ttaaataaag tagtgaaaaa taagaatcag tgctctaact gttcaagagg
       61 atcaagtggt tcaaatgttt ctgtagtctg ttttgcttcc aattggggca aaacagatcc
      121 aatttccaga tacttcactt tattcaatgt ataagataga atagaacagc taagtaatgt
      181 atacagaaaa tgtgtgagat ccaaataaag caaggagaca actaaaatac caaaagtaat
      241 caagaaaaga cttttgccaa tcttaatatg aaaatatttt tcaatggctt gataaatgat
      301 atctgttccg cctgtgcttc cgccgaatcg aaagacgaga ccgcaaccaa tgccagaaag
      361 aatacctgtc atcaaactat tgatcagtag actatctaca gcaaaatgta tatgaaggct
      421 ctcccataaa tgtaaaaaga aaactagcgc aaatgttccg acgatcgtcc ggagaaaagc
      481 gtgttttcca aaaagaaaca atgagataag caataaaggt aaattaaaaa gcagtgaagt
      541 gacagaagga gcgaatccaa aaagattatg caaaagcaaa gcaataccag taaccccacc
      601 atctgccagc ttagatggaa tattgattcc tgtcacagct aatccgtaga ttcctgcgcc
      661 taaaatgatt ccgcacaatt ctgtaaataa atttaatttt ttcatttcta gcctcttttc
      721 ttttgaaaat tttcagcaac ttctttatta tactgactta tactcttact tcaagaggaa
      781 aatgaaaaaa aattattttt ggctgtttct ttttatttat caaataagga gtggaaaaat
      841 gcgagcagca gacttattga caattcttac aaaatcaccc aaaaaaacag agtatcaagg
      901 agtttttttg gctcaagaac aaaaactggt gcctgtgaca atggtccaga taaataaagc
      961 aaatcaattt atctttttat ttgaacacgg caaagcacca cttgctatga aagacatctt
     1021 tctttttttg atgaagaacc gtgagaaagc tgtttattat cagtcaaaag aagaactcat
     1081 tcctctttat ggagtgaaag agatagaaaa taaagtggtg atataagtaa aaataagaaa
     1141 acaagacgtt tatgatgtca aaacaaccat tttttacatt tttggcaaaa aagtgtcatg
     1201 atacagtata atattcgttg aacaggccaa taaaggtgtt ctcccccacg agatttctac
     1261 tttattggct gtttcttttt attaaaataa aagttgtgtg tgtaaaaaag atatagctga
     1321 ttccaagtga caatttcgta tagattttta tggtattcgt agtgaaaaaa cgagaaccaa
     1381 aaagttgatc cgactttgtt gcttggattc tttttttgtc agcatattta gaggtatttt
     1441 tttctttttt ttgttcgtgc tgtaaggtta aggcaagaca agttagttgg gaaaaaggaa
     1501 agaaatgttt atacttaaag aataacagaa aggaaggtcg catgaataat tttatacaaa
     1561 cattaagtga aagaaaaggc gagttgctgg aagctgcatt tcaacatctg tccatttctc
     1621 tcatttcttt gctgattgca gcactgattg ctattccttt agcgatttgg gcagccaatc
     1681 ataaaaaaat ggcagaagtc ttattgcaga tcacgggtgt gctccaaacg attccttcac
     1741 ttgccttatt aggtttactt atcccatttg tagggatcgg aactgttcca gcattgattg
     1801 cacttgttat ttacgctttg cttccgattt tccaaaacac gtatattggt ctagctgaaa
     1861 ttgacccgtc tattgaagaa gcagcggtag catttggtat gtccagaatg agacgcttgc
     1921 tgaaagtaga gcttcccatc gcattacctg tcattatctc aggtatccga actgccttag
     1981 ttttaatcat cggtacggct actttggcag cattgatcgg agcaggtgga ttagggacat
     2041 ttattttgtt aggtattgac cgaaatacac ctgttttgac attgattggt gcgatcagtt
     2101 ctgccttgct tgcaattata ttcagcggac tgattcgctg gttgcagcat aagaaacctc
     2161 gatattccat cattacttta gcagcagtgg tgtgtttatt tggcggcgtt tctttatatc
     2221 agtctgcctt gtttaaaaat gaaaccatca cgattgctgg aaaattagga gcggaacctg
     2281 aaatcctgat ccaaatgtat aaagaattga ttgaagacaa cacaaaaaca gaagttgaat
     2341 taaaaccgaa ttttggcaag acgagttttt tgttcaatgc actggatcaa ggtcaaatcg
     2401 atatctatcc tgagttcaca ggtactgtcc tagaaagctt ggtgaaggta cctgaagcaa
     2461 tgaaaagtgc tgatttgaca aaagaaaaaa catatgacga agcagaagaa ttattgaata
     2521 agcaattcga tatgacatta ttaaaaccaa tggcttacca aaatacgtat gccttggcag
     2581 tcaagagatc ttttgcgaac gaacatcaac ttaaaaagat ttccgatttg gcagctatct
     2641 ctgatcagat caaagcggga ttcacactgg aatttattga tcgttcagat ggatataaag
     2701 gaatccaaga agcatatggc ttgaattttt caagtgtaca aagcatggag ccaagtttgc
     2761 gttaccaagc aatcaataat ggagatgtca atattgtgga tgcctattcc acagatagcc
     2821 agctcaagga gtacgatcta gtgactctag aagatgatcg agggttattc ccagcttatc
     2881 aaggcgcacc gctgatgaaa aaagattttg ctaaagaaca tcctgaaatc gttaaagcac
     2941 tgaataaatt aagcggaaaa ataacagagg atcaaatgat tgatatgaat taccaagtca
     3001 atgtcgaaaa gaaaaagccg acagaagtag cacgaatctt cctgaaaaat gaaggattag
     3061 taaaggagga agatcagtga ctaaattaat cgagttcaaa catgtgcaaa aaaagtatga
     3121 tggaaagtat gtcatcgatg acctaaatct ggcaatcaaa aaaggagaaa tctttgtttt
     3181 ggttggtcct tccggtagtg gaaaaacaac tactttgaaa atgatcaatg gattatctaa
     3241 accatcagca ggagacatct acttcaaagg aaaatcgtta aatgaatata atctgcagaa
     3301 aatgcggtgg aatatgggat atgtgctgca gcaaatcgct ttgttcccga ccatgacagt
     3361 caagcaaaat attgaagtca tccctgaaat gttaggttgg gaaaagcaga aaagagcaga
     3421 ccgtgtagat gaacttcttc aaaaagttgg cttatctcct gatatttatc gagatcgtat
     3481 gcctagagag ttgtctggag gagaacagca acggatcggg atcatccgag ctattgctgc
     3541 aagcccagat gtgattttga tggacgagcc atttagcgca ttagatccga tttcaagaaa
     3601 ctcattacaa gaattggttt tgtccttaca cgaagaattg ggcacgacca tcgttttcgt
     3661 tacgcacaat atggaagaag caatcaagct aggtgatcgt attgcattta tgaaggatgg
     3721 agaaattatc caatgtgaca caccagagca gttattgatg aatcctaaaa acgactacgt
     3781 tcggcatttc tttgatgaac cgaaacagac gaaagagtgg cgagtagaag atttagtcgt
     3841 caatggatat ttcttaaatg aaataccaga aaatgctcgt caacaagttt gctttgatac
     3901 gcctatgaaa gaagtatatt cgcttctttc ggtgtatcca agcattgtga tagtagaaaa
     3961 gcaaagaagt attgggtcgc tgacaagtaa agaaatcttt gcttttttaa gtcgagagga
     4021 ggaaggaaat gaaaacatat gatgcaatca tcatcggttc aggtgtgagc ggcttaagtg
     4081 ctgcttatgg attgaaagaa gcaggtaaaa cagtactagt ggtcgaagaa gatctatggg
     4141 gcgggacttg tcctaaccgt ggatgtgatc ctaaaaaagt cttgttaagt gcagtagagg
     4201 cacgtaatcg ggtaaaacaa ttgagtggaa aaggttttaa cgagatccca actgccaact
     4261 gggaagaact tcaaaaattc aaacgaacat ttacggatcc agtcccagaa agccgaaaaa
     4321 aacaattagc agaagctgaa atcgatcatc tttcaggaac cgctcgtttt ttggatgatt
     4381 catctattga agtcaacgaa gaagtcttcc atgcggatta cttagttttg gcaaccgggc
     4441 aaagacctac gatcttacca gtggagggaa aggaatactt gaaaactagt gcagactttt
     4501 tatcattgcc tgtactgcct aaagaaatca tttttatcgg tggtggttat attgcttttg
     4561 agctagctac gattgccaat gctgcaggga gcaaagtaac cattgtccat cataatcaac
     4621 ggccattaaa agaatttgag gcgtctttgg tagaggaagc cgtccatcaa atggaagcat
     4681 ctggcattca gtttgctttt ggtgttgaaa cccaaaagat catttcagaa ggtactcgct
     4741 atcgtttagt cggaaaagag acggaattag tagcagatat gattttttgt gcgactggga
     4801 gacagccgaa tacagaatct ttagcacttg agcaagcgaa tatcgtcttt gataaacacg
     4861 gaattgcagt caatgattat ctgcaaacga gcaatccaaa aatttttgcg tgtggagata
     4921 ttgtttcaag gaaaacccca aaattgacac cagtcgccac ttttgaagga aattatgtag
     4981 ctaagcggat aacggatgca acaagcgaac ctatcaaata tccgattata cctacgatcg
     5041 tctacgccag tcctaaactt gctgaagtag gagttacaaa aagtcacgcc tcttcgtcag
     5101 atcaagtggt agagatggat cttaccagtt ggttcaccta tcatcgtgtc aatgaaccag
     5161 ttgctaaggc tgaattgacc tttgaccagc aaaattattt gattggtgct gcagtgatta
     5221 gcgagcaagc agacgaattg atcgatgatt tgacactcgt catcaatcaa aaattgacta
     5281 aaaaagagtt ggatagctat atcatggggt atccgacatt ggcaagtgat ctctcctatt
     5341 tattgaaata atcagcatga taactaaagc gcttgttgct ttttccttct tgtgctacac
     5401 tttaaaaaat gaatacctgt ttctggggat gtcgtagacg acatgagagt aagttgtgaa
     5461 cttagaccca ttgaacctgt cagttaatac tggtgaaggg agaaacatag cagacgcttg
     5521 aagcgggtta ctatgtcgtg cccatttcaa gcgttttttg ttattcatat tagaagaaag
     5581 gaggtaacaa aatgaagaaa aatctttatc cagcagtcag tatggcattc attttaatta
     5641 tttggcagat tgttgtcata gtttggaaaa tacctgcctt tattttaccc ggacctgctg
     5701 ctgtattttc tgcattagcg gtagatgcac aagtattact ttcccattct gtcacgacat
     5761 taaatgaagc agtgcttggt ttggtaatag ctagcgttct ttcttttatt actgccttgg
     5821 caatggatta ttggaagttt ttagaaatgt ctttttatcc gttactagtg atttcacaaa
     5881 tgttgccaat catggtgtta gggccattac ttaccctatg gtttggtttc ggtatgcttc
     5941 caaaagtgat ccttgttgtc ttgataagct attttccgat cgtagtttct ttttcggata
     6001 cattgaggaa agtatcaaaa gagcaaatca tctttttgaa aacgatgggg gcagatacag
     6061 ggaagattta tcgaatatat aaaataccag cgggtttatc cggcttcttt tccggtttaa
     6121 aagtggctgc tacttattgt gtcagcggag cagtcgttgg agaatggcta agcgctcaag
     6181 caggcttagg gtattacatg atacgcgtaa aaaacagcta ccagatcgat aaagtttttg
     6241 ctgcgatcat ttgtgtcatt ctattgagtt tattgctgaa tggcgcttgt tcattattga
     6301 aaaaagggta ttatcgtatg ctttatattt tttaagagaa ggggatcaaa tgaaaaaatt
     6361 atggctttta ttaccgctac tgttgctttt atccgcttgt ggcactgcaa aagaaacaag
     6421 tagcaaacaa gaaataaagg atttgaaaaa agtgacactt gttttggatt atgtaccgaa
     6481 tactaatcat actggcatct acctagcaaa agaaaaaggc tattataaag aggctggttt
     6541 aaatgtccaa atcatcgaac caggagataa ttcgacaagt attggtctag taggtgcgga
     6601 caaagcacaa tttggtgtaa gttatcaaga agatgtcact tatgctcacg cagatggaca
     6661 gaatatacca gtcaaagcaa ttgctacagt gatcaaacat aatacttctg gattcgccac
     6721 cctatcagac agtaacatcc attcaccaaa agattttgaa ggaaaaacat atgcaggttg
     6781 gcaaagtcca agtgaagaag ctgtcttaaa agctgtgatg gaaaaagacg gcggggactt
     6841 ttcaaaactg acaatggtag gaagcaatgg agaagggcca gaaagcttag gaaaatcctc
     6901 agatatccaa tggtactttg aaggttggga tatgatcaaa gcaaaagaag cgggtattga
     6961 actaaactat atcccattga aagaattaga tgaacggtta gattactata ctccggtaat
     7021 tattacgaat gatcaactga tcaaaagtga tccagaattg gtacagtctt ttatggatgc
     7081 cacgaaaaaa ggctatcagg aagctattaa ggatccaaat gacagtgcta aactgttgca
     7141 aaaatatgca aaagaaaatg atcggacgtt tttagaagaa tcacaagcat tcttgtcaaa
     7201 aaattatacg gatgatcctg agaattgggg attgatggaa gaaaaagttt ggcgcaatta
     7261 tacagctttt atgcaggaaa acggattgat caagcaagat gtaccttcgc aagaactatt
     7321 cactaatcag tttataaaat aggagtgata gatatgtcaa tccaaatcag ccacttgaat
     7381 gtagtcattg aaaacctgaa aatattagaa gacatccatt tcactatccc taaagatacc
     7441 tttacatcga ttgtcgcgcc aagcggagcc ggtaagtcaa cattgctgaa aactttaaca
     7501 ggagtccagc ctatgacttc tggaacgatc aaagtagacg atcaaaaagt aactggtctc
     7561 catacagcat ttagttatat gccgcaagaa gatatgttac ttccttggct gagcgtctat
     7621 caaaacgtta cgttatatca aaaaatcaat catctaacaa tcaatgaaga acaagtaagg
     7681 gattatctgg atatctttgg gttaaacggc tatgaacatt ttttgccgga acaattatct
     7741 ggcgggatga agcagcgaac ggccttgtta cgaacaatca tgaatccttc ttcttacttg
     7801 cttcttgatg aaccatttgg agcgttagat gctatgacca gagggcagat gcaagactgg
     7861 atgttgaagc tgcctaaaaa agcaaagcgg actactttac ttgtgacgca tgatatcgaa
     7921 gaagcaattt atttatcaga caggattctt gtgctttccg cccgtccggc acatgtgatt
     7981 gctgagattc aagtgcctga aaaacgacgg tcacgtgaat ggctactgca gcaatcagag
     8041 ttgaagcaaa cgatttatca gttattggca ggtgaattag atgttaagtg atgctcattg
     8101 tcatttagat gggagtcaga gtttagcact ccttcagcag gaacacacga ttttgacgat
     8161 catcaattgt gattcgccag aagaatggaa ggaaaacaga cagcttgcag caagtaagac
     8221 tcaagcctta agttacggta tccatccttg gaaagcagat agctatacgt ttgaacaagt
     8281 tgagcctttt ttgaaaaaag caaggatcat cggtgaaata gggttagata atatttggac
     8341 gaatgtaccg atgactacac aaaaaaaagt atttgagcga cagcttgctt ttgcagccat
     8401 aaatgagaaa cctgttgtac tgcacacaaa aggatgcgaa aaagagatct taaattatat
     8461 ccaagcttac cctaatcact atttgatcca ttggtattct tctttggact atcaacaaga
     8521 ctatattgat ttaggctgtt atttcagcat tggtcttgat ttgaaaaaga atccagcagt
     8581 ctggcaacta gctaaagcag tcccaatcga ccacttactt attgaaacgg atggactaca
     8641 tgcagcagag tgggctttag gggggatttt ttcagaaaaa gattatgtct cactgcttga
     8701 agaacagtta aagacaatcg ctgagatcaa agagctatca gttgaagaag ttaaaaaaaa
     8761 tacttatcaa aacttggaac gtttcattag attaggtaaa taaaaaagag atgtgacaca
     8821 actagtgtcg cacctctttt tggctttctt tgtataaaag taaactgttt gattaataaa
     8881 tgaatcttag taccaaccgt ttgcttccca gaatgctttc gccgcttccc aagaaccata
     8941 acgtgaagta acatattgtt ctgctactct ttcttggttt gcagcagagt agtcaccatt
     9001 taagtaagaa gaatctaatt ggtaacgtcc gatgtaacgt ccgttcgttg ctgtgtaaga
     9061 accgcttgat tctttttgtg cgatccattc ttttgcagag cttgtgcttg ctggagtagc
     9121 agcagtttct gttacagcag gagcaggttg tgtttcagca acaggcgctg cttgtttttc
     9181 cgtttgaacc gtttcttgta caacaggttg ttcagcttgg actggtgctt cttgaacggc
     9241 tgcttgaggt tcagcagctg cttgtgcaga gccttctgtt gggattgtta attgttgtcc
     9301 tgagta
//
LOCUS       DAZUMM010000091         9019 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000091 DAZUMM010000000
VERSION     DAZUMM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.094"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..974
                     /locus_tag="ACG8E6_002292"
     CDS             168..974
                     /locus_tag="ACG8E6_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5062676.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            986..2206
                     /locus_tag="ACG8E6_002293"
     CDS             986..2206
                     /locus_tag="ACG8E6_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5062677.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2196..3782
                     /locus_tag="ACG8E6_002294"
     CDS             2196..3782
                     /locus_tag="ACG8E6_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5062678.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3821..5959
                     /locus_tag="ACG8E6_002295"
     CDS             3821..5959
                     /locus_tag="ACG8E6_002295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5062679.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSVIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            6247..7638
                     /locus_tag="ACG8E6_002296"
     CDS             6247..7638
                     /locus_tag="ACG8E6_002296"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS5062680.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            7697..8506
                     /locus_tag="ACG8E6_002297"
     CDS             7697..8506
                     /locus_tag="ACG8E6_002297"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5062681.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            8478..>9019
                     /locus_tag="ACG8E6_002298"
     CDS             8478..>9019
                     /locus_tag="ACG8E6_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5062682.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFP"
BASE COUNT         3087 a         1393 c         1838 g         2701 t
ORIGIN      
        1 cttttttggt ttattgcaat tgtctagcat cttctgtaaa atagaaacgt tagagaaaat
       61 cttgtatcat agaaaattaa gaaagaaata agaacgcagc tcggtaccaa aaatatggta
      121 aaatggcact tgactgtaat tcactgaaat cgcttggagg aacacaaatg tttaaagatt
      181 tgtatttatt tatgaaagac atttatcaga atagaaaatt actgctacag ttttcgatca
      241 atgacttcaa atcaagatat gcaggatcgt ttttaggaat cttatgggcg tttatcaatc
      301 cgctatttac tgtattgatt tattggctgg tatttggtgt tggactgaaa gcacaaatga
      361 tcgatgcaaa atacccattt atcgtatatt tagttaccgg gatgattccc tggttcttct
      421 tctcagatgc atttgcatca actacggttg tttttcggga atatacttat ttagtaaaga
      481 aagtcgtatt caatattcgt atcttgccca cgacgaaaat cctatccaat ctgtacaccc
      541 atttattctt cattctgatt ggcatagctg tcgcgcttat aaatggtata tatccttcag
      601 caatgtcttt acagctgatt tattatttat tttgcatgtg tgctttcttg acaggattga
      661 cttggctgac ggcatcgatc caaccatttt tacctgatat catgcaatta attacgatcg
      721 tgctgcagtt gatcatgtgg acattaccga tattatggag ccctaatcag tttgattcag
      781 gtcttgtaag catattgaaa ttaaatccgc tgtactatgt tgttcaaggt tatcgtgagt
      841 catttttaag tgaagcttgg ttctgggagc atggaacata tactttgtat ttctgggttt
      901 tcacgttagt aatgttcgta atcggctctg cagtgttccg tagattgaaa ccacattttt
      961 cagatgtgct gtaaggagaa tagctatgac agaatatgca attagactaa aagatatcac
     1021 aaaatcttat aatatgtacg caaaaccttc ggatcgattt cgtgaagccc tcaatccttt
     1081 taagaaatcc tatcatgatg tattttatgc gttgaaaaac gtgaatgtag aaataaaaaa
     1141 aggtgagatg atcggtttcg tgggagaaaa cggatcggga aaatctacaa tgttgaaaat
     1201 cattacggga gtactgacac caacatccgg cacgatggaa atcgaaggaa aaatctcagc
     1261 tttgctagaa ctaggatcag gattcaatcc tgagtattct ggctatgaaa atatctattt
     1321 aaatggaatg gttctaggat tttcaagaga agaagtcgat gaaatggtcg atgatatcat
     1381 tgaatttgct gatattggcg accatattta tcaacctgta aaaacctatt caagtgggat
     1441 gttcgttcgt ctagcttttg ctgtagcgat caatgtgaat cctgatattt tgattgtgga
     1501 tgaagcattg gcagtaggag atcttgaatt ccaactgaaa tgtatggaaa aatttacaga
     1561 aattaagaac tcgggaaaaa cgattttatt cgtttctcat gatattagtt cgattcgccg
     1621 tttttgtgat cgttcctatt ggttgaaaaa tggagaagtt gtcgaatatg gtgatacgat
     1681 ggaagtaacg acgaattacg aaaacttttt gaagaaaaaa tcagttaaaa cagtagatcg
     1741 aaataaaaac caagtagaac gcttcgcagc tccagatatc gtagacgtga cgaaagccga
     1801 attgttggat aaaaataaaa atccagtgga tatgctggaa caaggagaaa aactatacgt
     1861 taaagtaacc tatgaagtaa aagacgatac aatcaaaaat ccagttttag gaattgctct
     1921 gcgtacagtc gacaattttt atgtgtgcgg actcaatact ctactagatg gtatcaagat
     1981 cccttggaaa aaaggtgaga atatctttta tctggagtat gaaaagttag gtttgttagc
     2041 tggtgaatat tattttgacg ttgcggtctt tgaagaaaat gcaacggttc ctttggtata
     2101 caagacaaaa tacatgaact tgtttgtcag cggttcttat atcggagaag gcatcgtggt
     2161 cttagaccat aaatgggaag aagggactca tagcaatgaa atatgatttt gaaatggacc
     2221 tagatgaaca aagcagtgtg ggaaaaattg ccgcacagat caaaccagga agtaaagttt
     2281 tagagtttgg tcccggaaac ggtcgattaa cgaaacattt gatcggagca aaacaatgtg
     2341 aagtgagcat tgttgagcta gataaggaat tattcgattt tgtcagcgaa tttgcccaag
     2401 atggatttta tggagatatc gaaagttttg aatgggcaaa ctattatgct ggacagacat
     2461 ttgattacgt cttgtttgcg gatgttttag aacatttagt agatcctgga aaaacattga
     2521 agaaagtcag agaatttttg aatgaagaag gagagatcct gattacattc cctaacttag
     2581 cacataattc tgtcatgatc gatttgttca ataatcaatt gccttgggct tcttatggtc
     2641 tcctagatga aacccataat tctttttata ctcatgatgg tttccaaaaa gtttttgaaa
     2701 aagccggatt attcattaat attgaagatt acttgtactt ggctgtagga gatacagaat
     2761 taaaatcaac ttatgaagag ttgccggaag ctgtacgcta tgatttcaaa atgcgaccat
     2821 ttggtgaagt ctaccaatac tttttctcat tgatgaaaca tccagtagta caaacttcca
     2881 ttgcacagcc acaaaactct aattatgtaa aagtactaga agtaacccag caaacgaaac
     2941 aagatgaaac aatccaacaa ataccattca ataatttcac aggggaaaat gagacactaa
     3001 gtttccccgt gtcagaagca acagaaagaa tggtgtttcg cttcgcacag caacctagct
     3061 ttatcgaatt cagtgcagaa gctgctggag aaaaactcat atttatcgat tcaaatgctg
     3121 tagtcaaaac agtaaatgat tgttatttgt ttgatgggaa agaaatacca gagttcgctc
     3181 taacggatat ttctgggaaa gaagtgacta tccattgtca ctatcgcttt atcggggaat
     3241 tgacaccaac aatgaaagaa ttgctggaaa caattcgtcc tatggctgaa gtcacaaagc
     3301 agctttctga gaaaaatgat gaattagaaa gagaaaatca gcatcttctt gaggaaaata
     3361 caagactaga ccatacgtta aaaacaacca caaatcgcta ttgcacattg ttggaaagtg
     3421 atgaatggac gatcaaacat agactaggac gacgtaagaa aaaaacatcg aaaaaaatac
     3481 aagaaaaaga gctttctata tgtattgatg agaaaatatg ggatgcagaa acaaaaatat
     3541 taaaaatcat cggatgggga atcgctaatt cggatcgcca gccattgtcc tataagttat
     3601 cagctgacca atcgccattt ttcgaagcaa ttcctgtttc acgagatgaa gtcaatcaag
     3661 cagaacagct cgcaaaagga acagaagcgg gatttgaatt acgaatcctt tgcgagcaag
     3721 aacgttcatt cttagtggaa gcagtagctc aaaatggtca atcatggatc attgaaatgt
     3781 aaataatgat taacttataa agataaggag taaacgaatc gtgcaaaaaa aagaaattgc
     3841 cgtatttatc gatcaaatca ctcgtgaacg ggccactggc gatttgctta ttgtgggctg
     3901 ggcaattgat gaagtgacca aagaaatccc tacgataaag gtagaaaaag aaaatgtgat
     3961 tgctgaagca acacatgtcg taagattgga tatcaatcat ttatataatc ttgatgttaa
     4021 gacccaaagc ggttttaaga tacgtttatc cgggaaaatg agaggaaaag cgatcttaga
     4081 ttttcaaacg gcaaaacatc aaaatggcat agctgtgaaa ctaaatggta aataccctta
     4141 tgatgacggc attgaatctt catgggaaag aaaaaaaaga ttactgaaaa aagggataaa
     4201 ttatgcaagg actcatggcg taaaaaaaac aatccgccgg gtcaaactgg aattgaaacc
     4261 aggatcgatc gactatgcag agtggatcag tcgtcacgag aaacctgatc tgaaagagca
     4321 aaggaaagaa gcacaagggt ttgcataccg tccattgatt tccatactta tgcctgtcta
     4381 taacgttgaa atcaaatggc tcgaaaaatg cattgattcg gtattggatc aaacgtatga
     4441 tcactgggaa ctatgtatca gtgatgatgc ttctactgat ccagctatcc gaaagtgtct
     4501 ggaatcctat caagcaaagg atgatagaat caaagttgtt ttccgacaag aaaatggaca
     4561 tatcagtctg gcaactaatt ctgcgttgga aatggcagaa ggagaattta tagcgctctt
     4621 agataatgat gatgaattac cgccatttgc attatatgaa gtggcaaaag tgctaaacat
     4681 acatccagaa ctggatttga tctacagtga cgaagataaa atcgatgcag atggcaatcg
     4741 ctttgatccg cacttcaaag cagactggtc tcccgataca ttgatgggaa ataattatat
     4801 ctctcactta ggcgtatatc gaacaagtat tgtgaaaaag cttggcggtt tccgtaaagg
     4861 ctacgaaggc tctcaagatt atgatttggt ccttcgtgtg acagaacaaa tccctgcaga
     4921 tcatatttac catattgacc gagtcttgta tcattggcgg acgatcccag gatcaaccgc
     4981 aagtaatggt gaagccaaaa gttatatcta tgattctgga gtgaaagcat tgacagatgc
     5041 cctgtctaga aggaatatca aaggaagcgt acgtcctggc agaatttctg gattttatga
     5101 gattgcttat gacgtcttgc aagaggattt ggtcagcgtg atcattccaa cgaagaatgg
     5161 ctatgaagat ctaaaaactt gtgtggattc ggttatcgaa aaaacgtctt atccaaatta
     5221 tgaaatcatt attgcagata atggaagtac agatcctaaa atgcaggaat tatttgcaga
     5281 atacaagcat cagctgaaag accgatttat cgttgaatta attgacattc cattcaatta
     5341 ttcacgtatc aacaatctgg cagcagaaaa agcaaacggc aaatactttt tattcttgaa
     5401 taacgataca gaagtgattg aacctgactg gatgacagcg atggtttctt atgcacagtt
     5461 tgatcgaatc ggctgtgtag gagcaaaact gttttatcca gatgatacaa cgcagcatgc
     5521 aggagtatta ttaggaatcg gtggtgttgc aggacatgca ttgaataact atgatcgaac
     5581 acattgtggt tatttcggcc gattggtcat tgacgtcaat tacttagcag tcactgcagc
     5641 ttgtatgatg gtcaaggcag ctgacttcaa tgctgtcaat ggatttgatg aaacattaga
     5701 agtagcattc aatgatgttg acttgtgttt gaaagtttat gaactgggtc gttacaatgt
     5761 ctatgcacat caagcagaat tgtatcattt cgaatctaaa tcaagaggtt atgaagatac
     5821 accagaaaaa caaagacgtt ttgccgggga aataaaaaaa atgcaagata agtggccttc
     5881 ttatattgct catgacccat tttataacga taatctaact aaagaaggaa tcggcgattt
     5941 ttcattgaga cctgattgat taaaagaact gtattttcat ttttattgat gaaaatacag
     6001 tttttttagt atatgaaaat tgtaacttgt gaccgaagag atgataggaa gaacacccat
     6061 tcagtaggaa gaatgtgata cgatttttgt ccaactctct gttaggcaga aatatacata
     6121 taaacataca atagtcacct tttctagttt cttttaattt attttacaat ttggttattc
     6181 actaggtttt taaactttgc tatggtaaga tgatagaggc attattttat ggttaggagt
     6241 gtatatatga ataaaaatgg ggagtggaat gcggcacgta ggatattcat tatcatcatg
     6301 gatgtacttg tattgaatgt gtcgatctat cttagctttt tgctgaagtt ccaaggagaa
     6361 gtcccagcaa gaaacttaga gacctttcag cattcagcag tttttatttc cattatcttt
     6421 attgcgttga atattttgtt aggagcttat gtattctata gccgagtgat cagtgatatc
     6481 gtcttcatta ctgttattgg acaggtgctg atagcattgg ggatcatggt agttactttt
     6541 gctggacgtt ggtttgcatt tccgcgtggc gtcatcgtgc tctcgtttat acttggcacg
     6601 atattactta gcgtttatcg gatagttgtt tataaactat acttaagagt cagtcgtgat
     6661 aagaaagtcc ttatcgtagg tttggagaaa gatgtcgcac cagctatcca aaactttcaa
     6721 tctaagaaaa acaacaaaca taaagttgaa gcagtagtga tcgacaagta ttacgaaaac
     6781 acaaaaaaat tgattgatga tatcgatatt gtctacttgg cttctaaaat cccagaagac
     6841 gaaaaaatca atatttatca attagtaact aaaaaagaga agaagctatt tttaaataca
     6901 acttttgaaa atttaacgat gatcaatccg aacatcatga atttcgaaga tgaaagtatc
     6961 attgaagctt ccggtttcaa aattcagccg gaatatgaat tattcaaacg tctttttgat
     7021 atcatcgttt cgcttatttt actgattctt gcttcacctt ttatgctaat cacagcgatt
     7081 ttggtaaaaa caacttcacc tggaccaatt atttataaac aagtacgtat tacgaaaaat
     7141 caaaaggaat tttcaatcta taaatttcgt tccatgagtg taacagctga agcaaaatca
     7201 ggaccggtct tggcaaagag caatgatgca cgtgtgacac cagtaggcaa atttatccgt
     7261 gccgtacgtt ttgacgaatt acctcaaatc tttaatgtat tgaaaggcga tatgtcgatc
     7321 gtcggaccac gtccagaaag accatttttc gttgatcagt tcaatgaaga aaatccttat
     7381 tattatttaa gacacaatgt acgtgcaggg attaccggat atgcacaagt atacggaaaa
     7441 tatgtatcag attataatag taaactacgt tttgatttgt tgtatatcaa aaagtattcc
     7501 ttactattag atttgaagat tttacttcaa acaatcaaaa tcttatttga taagatgtcc
     7561 tcacaaggac tagatgaaga tgaaaatatc cgttcgttag cagaaatcga cttttcacaa
     7621 gagaaaattt atcgctagat gggaaaaatt ttgagcaaag aagtaggaga gaacattgga
     7681 aaataaaaat ataagtatgc tgctttcttt atatgataaa gaaaaaccag agtatttaaa
     7741 ggaagcatta actagtattt ttgagcaaac agtaatgcca aaagaaattg tattagttta
     7801 tgacggccct ataaatgaaa aacttcaagt agttgtcact tatttccaac aaaaagatcc
     7861 tcacttgttt acagttgtaa aattagaaaa aaatcaagga ttggggattg ctttagcaat
     7921 tggattagaa catacgaaaa atcgcttagt agccagaatg gatacagatg atatcatgga
     7981 accccaacga ttagaaaaac aattagagat cttccaaaaa gatccagaaa tagcgattgt
     8041 tgggagtaat attgaggaat ttactggcac gcttagcaat gttatcggaa gaaggattgt
     8101 acctgaatat aatgatgaaa tccgtgtgtt ttcgaaaaga agaaatccct ttaatcatat
     8161 gacagtcatg tttgataaaa atgctatttt agaagtggga aactatcgcc cattaattgg
     8221 atttgaagat tattatttgt gggtaagact gttgaaggcg ggatataaag ggtataatat
     8281 ccaagagatt ctagtccatg ctagagcagg tgctgacatg tatgctagac gtggaggaag
     8341 taaatatctt ttaccaggaa ttaaaggccg atatcgcgta tggaaagatg gtctaggatc
     8401 actaaaagat tttctgatag ttatttgcgg acatcttgta attagtttat taccgaataa
     8461 aattagggga aaagtatatg aatcaaaact tagaaaaaca aactaaactt tctataatca
     8521 taccttttta caatgcaaaa aatactttaa aaagagctgt agacagcgtc ttaacgcaag
     8581 gttatcccaa tattgaaatc ttgttaattg atgatggatc tacagatggt agtagtgaca
     8641 ttggtgacga aattgctaat ctagagagta aagtagcatg ttatcacttg gaaaacgctg
     8701 gtccatcagt tgctaggaat tgtgggataa aagaagcaac tggaaatttg gtaggatttt
     8761 tagatagtga cgattatttt ttacctgatt gttttaataa tgttttgaaa gactaccata
     8821 ggaattaccc cgatatcatt gcttttggat taagtaaagg aaaaaatata gaaaatgcaa
     8881 atttatttgt tcccgaaaag aagattggtt gttcaagtga agaagcaatt gagcaaatgt
     8941 ttagaagtaa ggcagtggat ttttacgtat ggaataaatt ttttaaaagg gaattattca
     9001 atcatatagt ttttccaga
//
LOCUS       DAZUMM010000092         8984 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000092 DAZUMM010000000
VERSION     DAZUMM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.095"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..1154)
                     /locus_tag="ACG8E6_002299"
     CDS             complement(15..1154)
                     /locus_tag="ACG8E6_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFS5062683.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1553..2851)
                     /gene="eno"
                     /locus_tag="ACG8E6_002300"
     CDS             complement(1553..2851)
                     /gene="eno"
                     /locus_tag="ACG8E6_002300"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFS5062684.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2967..3722)
                     /gene="tpiA"
                     /locus_tag="ACG8E6_002301"
     CDS             complement(2967..3722)
                     /gene="tpiA"
                     /locus_tag="ACG8E6_002301"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFS5062685.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3826..5016)
                     /gene="pgk"
                     /locus_tag="ACG8E6_002302"
     CDS             complement(3826..5016)
                     /gene="pgk"
                     /locus_tag="ACG8E6_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFS5062686.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5126..6127)
                     /gene="gap"
                     /locus_tag="ACG8E6_002303"
     CDS             complement(5126..6127)
                     /gene="gap"
                     /locus_tag="ACG8E6_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS5062687.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6168..7259)
                     /locus_tag="ACG8E6_002304"
     CDS             complement(6168..7259)
                     /locus_tag="ACG8E6_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFS5062688.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7408..8742)
                     /gene="rpoN"
                     /locus_tag="ACG8E6_002305"
     CDS             complement(7408..8742)
                     /gene="rpoN"
                     /locus_tag="ACG8E6_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFS5062689.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8802..8873
                     /locus_tag="ACG8E6_002306"
     tRNA            8802..8873
                     /locus_tag="ACG8E6_002306"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8835..8837,aa:Arg,seq:ccg)
BASE COUNT         2330 a         1895 c         1659 g         3100 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgtccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tattttattt atacgagagg acttgcccat agattcctat
     1321 tctactgttc tatccttaaa agctcttata tgatgctagt tattgttagc tttccttttt
     1381 caaggatgat cttttcttgt accattcttt gtaaacatca tatacagaag ctagttgcaa
     1441 aaaaactctc tacctgctga tatttcagca agtagagagt tcgattactt aactaagtga
     1501 tcatactcga ctatctttca atagtcgatc atgtatcttc attagaaaag aattatttgt
     1561 tttttaagtt gtagaaagat ttcaaacctt tgtattctgc aacttcacca agttggtctt
     1621 cgatacgtag taattggttg tatttagcaa tacggtcagt acgtgataga gaaccagttt
     1681 tgatttggcc agcgtttgtt gcaacagcga tatcagagat tgttgaatct tctgtttcac
     1741 cagaacggtg agatacaact gctgtgtagc cagcttcttt agccatttcg atagcttcga
     1801 aagtttctgt taatgtaccg atttggttca ctttgataag gattgagtta gcaattcctt
     1861 tttcgatacc ttcagataat ttttgagtgt ttgttacgaa caagtcgtca ccaactaatt
     1921 gaactttgtc gcctaatacg tcagttaatt tcttgaatcc gtcccagtcg ttttcgtcta
     1981 atccatcttc gattgagatg attggatatt tagaaactaa ttcttcgtag aatttgatca
     2041 tttcgtcagt tgttttttcg ccttcgcctg agtcagctag tacgtaaaca cctttttctt
     2101 tgtcgtagaa ttctgaagaa gcagcatcca tagcaagaac aacgtcttta ccaggtacat
     2161 agccagcttt ttcgattgct tcgatgatta cttcaaaacc ttcttcgttt gaaccaaggt
     2221 ttggagcaaa tccaccttcg tcaccaacag aagttgctaa tccgcgagct ttcaagattg
     2281 aagctaatgc gtggaatact tcagcaccca tacgtagagc ttctttgaat gttggagcgc
     2341 caacaggcat gatcatgaat tcttggaagt cgattgagtt atcagcatgt gatccgccgt
     2401 tgatgatgtt catcattggt gttggcaata cttttgtgtt gaatccgcct aggtagtggt
     2461 ataaaggtac ttctaggtag tcagcagctg cacgtgcaac agcgatagaa acacctaaaa
     2521 tagcgttcgc acctaattta cctttgttag gagttccgtc taaagcgatc atagctttgt
     2581 cgatagccat ttggtcacga acatcgtagc caatgattgc ttcagcaatg atgttgttta
     2641 cgttgtcgac agctttagtt acacctttac cgccataacg agctttgtct ccgtcacgta
     2701 attcaactgc ttcgtattca ccagttgaag cacctgaagg aaccattccg cggccaaaag
     2761 ctccgctttc tgtgtatact tctacttcga ttgttgggtt accacgtgag tctaagatct
     2821 cgcgagcgta aacatcagta ataattgaca tgttttgtct ctcctttgag tttgtttttt
     2881 cctaagggat ttccctcata tacgatttta gttaattttg ctcattagtg caaacatttc
     2941 ttgcattttt cgcaaatttt caacaattat tttacagcgt ctaacaatga caagaatgaa
     3001 tctgcttgca agcttgcgcc accgactaaa gcaccgtcaa cattttcttt tgccatgtat
     3061 tcagcaatat tttctggttt tacagaaccg ccgtattgga tacgaactgc ttctgatact
     3121 tcttttccat aaagtttttc aactgtttga cgtacaactc cacaaatttc gtcagcgatt
     3181 tgtgcgtcag ctgatttacc agtaccgatt gcccagattg gttcataagc aataaccatg
     3241 tttgacactt gttcagctga aagatcagct aaaccagctg taatttgtcc ttcgatccat
     3301 tcagcagttt tgcctgcttc ataggtttct aaagattcgc cgcagcatag gattggtgtc
     3361 ataccatttg caaagatagc ttttgctttt ttattgatgt cttcgtctgt ttcatggaaa
     3421 tattcacgac gttctgaatg tccgatgatc acataatgaa cacctagatc agcaagagct
     3481 gctggtgaag tttcaccagt gaacgcacct gagttttccc agtagcagtt ttgtgctgag
     3541 atttttagtt ctgtaccttc agctgatgtc attaattctt gtaagaataa tgcaggagat
     3601 ccgatgactg aatcaacttt gtcatttgaa gggattttgt tttttactgc ttcagcaaat
     3661 tcttttgctt ctgatgcagt tttgttcatt ttccagttac ctgcgataat aggtttacgc
     3721 attgaggaac acactcctta atttattgaa aaatggctag ggagtcatga aaactccctt
     3781 caccttttga tcgattactc gtcagaattg agcaaacttg gtcgtttatt tatcgttgat
     3841 tgctgctagt cctggtaatt ctttaccttc aagcaattcc aagcttgcgc caccacctgt
     3901 tgagatgtgt gtgaatttgt cagcgaatcc taattgttct gctgctgcag ctgagtctcc
     3961 gccaccgatg attgttgttg cgccttctaa gttagcgatt gcttcacaaa caccgattgt
     4021 acctttagca aagttactca tttcaaatac acccataggt ccgttccaaa caactgtttt
     4081 tgcaccttgt aatgtatcag caaataattt tactgatgct gggccgatgt ctaaagccat
     4141 caaaccatca ggaatgtctc cttcgattac ttgtgtttca acatcatttg aaaattctgg
     4201 tgcacagaca ttgtcgattg gcaagacaag tttgtcgcct gctttttcga ttaattcttt
     4261 tgctagttct actttatcag cttctactag agagttaccg atcttgattc cttttgcttc
     4321 gtagaatgtg taagtcattc cgccaccgat taagatttta tctgctttag gaatcaagtt
     4381 ttcgattacg ccgattttat cagaaacttt tgcgccacca aggattgcaa tcattgggcg
     4441 ttttggttct tctactgctt caccgatgaa cttgatttct ttttccatca agaatccagc
     4501 aactgttggg ataccagttg aagcgatacc tacgttagaa gcatgtgcac ggtgagctgt
     4561 accgaaagca tcgttaacaa atacatcgcc taatgaagcc cagtatttac ctagttctgt
     4621 atcatttttg ctttcttttt taccgtcaat atcttcaaaa cgtgtatttt cgaatacgac
     4681 aacgtcgccg tcattcatgt tgcggattgc ttcttctaat tgttctccgc gagtttcagg
     4741 tacgaatgtt acttctttac ctaataattc gcctagacgt tttgcaactg gagctaaaga
     4801 ttttccttct ttatcttctt ctgtttttac acgtccaagg tgagagaaaa ggattgcttt
     4861 tccgccttgt tctagaacgt agttgattgt tggcaatgct gctttgatac gagtgtcgtc
     4921 agtgatcacg ccgtctttca aaggaacgtt gaagtcaaca cggacaagaa cttttttgtc
     4981 ttttaaatcg atatctttca ctgttttctt agccatttta tttcctcctg aataaactta
     5041 ttatcaaaaa aagcggaaga agcgcgattg cttccccgct tttaaatgta catgtctata
     5101 atttacactt gtacgtttgt ctatcttata agttagcgaa gtattctaaa gtacgtacta
     5161 attgtgcagt gtatgacatt tcgttgtcgt accaagcaac agttttaact aattgtttgt
     5221 cgccaactgt cattacttta gtttgtgtag catcaaataa tgaaccgtaa gtcataccaa
     5281 cgatatctga ggaaacgatt tcgtctgtgt tgtatccgta agattcgttt gaagcttctt
     5341 tcattgctgc attgatttcg tcaacagtta cttctttttc aagaactgtt actaattctg
     5401 ttaatgaacc tgttggtaca ggaacacgtt gagcagctcc gtctaattta ccgttcaatt
     5461 cgggaattac taaaccgata gctttagcag cacctgttga gttaggaacg atgtttgcag
     5521 cagcagcgcg tgcacggcgg aagtcacctt taggatgagg tccgtctaga gtcatttggt
     5581 cacctgtgta agcgtggata gttgtcatta atccttcaac aacaccaaat ttgtcgttca
     5641 atgttttagc cataggagct aaacagttag tagtacatga agcacctgag ataactgttt
     5701 ctttaccagt taatgtttcg tggtttgtgt tataaacgat tgttggaaca tcgtttccgc
     5761 caggagcaga gataacaacg cgtttagcac cagctgttaa atgtttttca gctgctgatt
     5821 tagaagtgaa gaaaccagta cattctagaa cgatatctac gcctagttcg ccccatggta
     5881 attcttctgg gttgcggtta gctaatactt taacttcttt tccgtttacg ttgaatgaac
     5941 cttcatgaac ttcaactgtt ccgttgaaac gtccttgagt tgtgtcgtat tttaacaagt
     6001 gtgccaacat tttagcatct gtcaagtcgt tgattgctac tacttcgatt ccatctacat
     6061 cttggatacg acggaatgct agacgtccaa tacgtccaaa accattaata cctactttaa
     6121 ctgtcattta agatttcctc cttatgaaaa tcaaaaaaat atttatttta aagggttacc
     6181 ccttttaaaa tctcatttgc agcagcttca tcagtgatga gccacgtttg ttttggtgca
     6241 tttttcatat aagctcgaat tgctttagct tttgattttc cgcccgcgat tgccatgatg
     6301 atcgggactt tttccaaatc ttgcaattct atgccgattc tagggacctt ataaacgact
     6361 tcaccttttt cattgaaaaa gtagccaaaa gattccgcaa cagcatttgc ttgtttcagc
     6421 atgactaatt ctttttcagt catcttacga cgtgctgcca tgtgtaatgc acgcccaata
     6481 ctatgaataa cacaattcgc ttcactaatc aagtttaaga cttcttggat agatggctca
     6541 ttgagtaaag aattgtaagt tgccagactt aattgttccg gaacataaag tgcgcggtgg
     6601 ttaccgtttg ctttagcagc cattcttgca ctgacagaat ttgcctgtac agttatcgct
     6661 tcaccaatcc cgcctcttgc agggacaaac aggttatgcc gtttcttatt ttccagatta
     6721 gacaattgtt cggctaccac agccatagta gttcctccca tcacagcgat gatattctcg
     6781 ccttctggaa gtagtcggtc taaggcttcg ctcagcgctt cgccgaatgc atcagccact
     6841 ttggtctgct cttcgctgtt tcctgaaaca acgatacagc gttggttacc aaaatattct
     6901 gccaactgtt gttcggtttg atgcgtacct aaaagctgat ccatgaagtt ttcaaggctt
     6961 tgataaactt cttcgccctt agttgtcagc gtcataccgc tttttgaggt gtcgatcaaa
     7021 ttcagatttt tcaaaaggtc tgtttctgtt cgtaagactc gttctgtgat ccccatactt
     7081 tccgataaac ttcgacgccc aatcggctgc atccaataga tatttcgtaa aattttatag
     7141 cgttcttgca acacttcgat aatatctggt gcaacagatt caatcatttt tatttcatcc
     7201 agcatagcga tctccttagt tggacacttt acgtccaatg tagacttaaa acgacccata
     7261 tacgataaaa aaagaaatta actattcgaa tttcacatca ataaagggaa atctcaacct
     7321 cacgactgta agtctaacag ttttgctctc gtttttcaac taaaaagact agaaaaagtg
     7381 aaatactttt tctagtcttt tgactgatta gttatcataa cgttttcttt ttgatgaact
     7441 cgggattcct aataaatcac gatattttgc tactgttcgt cttgagatcg ctatctcatc
     7501 ttttttcagc aattcaacca atttctggtc cgacaacggc ttgtttttgt cttcttgatc
     7561 gactaattcc tgcagctttt tcttcgctgt atctgctgaa aggtcttctg tctgttctgt
     7621 actattcgta ggaattcgtg tagtgaaaaa tttttttaat tcaaatacac cgaaatctgt
     7681 ttccaaatat ttcccgttga ctgcacgact gaccgtggat tcatgcacat ctatctcagt
     7741 tgcaacttcc tttaacgtaa gaggcttgat tggacgttcc ttgtcgataa agaaagcttt
     7801 ttgccgggaa acaatgactt gagcaacccg caaaattgta tccccacgtt ggaggatcgt
     7861 tttcttcaac cattcgaatt cctgtttccg ttcttttaaa taattttcag tttctttatc
     7921 tgcttgttgt gacatttgtt tgaagtaact ctcttgaaag cgtatatttg gctgattttt
     7981 tcggtttgag ataacttgta catttttatc gtcgtccact aatacccgga catcaggaat
     8041 aatgtacaat tcgctgctac ggtcgaaaat ccggccaggg cttggactca acgtttgtaa
     8101 atagtcgaat atctgctgaa cagcatgtag atcaacagaa aaacgttgtg cgatttcttt
     8161 ccattttcgc tccaccagtt cctcaaaaca ttcttccaac acgatataag ctaattccgg
     8221 cgcataatca tctcgttctg tttgaagcat gagacattct tgcaaactac gtgcacctac
     8281 tccagctggt tctaattgct gaatcagagt caacgcatcg agcatttgga taggcgtagc
     8341 tccaatctct tttccggctt cttctaaact aatcgtcaaa aatccgttca aatcgatgta
     8401 ttctacaaga taaagaacga ttttcctcaa aaaagtatct cggtaattca aatggacttg
     8461 ttcgatcaat gaatcaaata aagacccttt tgtatcagga atctgattta ggtaatttgt
     8521 ctcttctcct tcataagaag atgaagaagc ttttatgtaa tctggctgcc attccggctc
     8581 aacaacttcc accaatggat tttccattgc ttgattctca acgaatgcca acagctcctc
     8641 actgttatat tgtaaaactt ggatggactg ttgcaactgt tgcgtcatgg ctagtttctg
     8701 tgtctgctgc tgtttttgtg aaaaattttg ctcaaatttc atttttttgt ccaatcccct
     8761 tgtaattttt tatctgttta gttacaatta tcccagtgct tgcgtcctta gtgtagtgga
     8821 tatcacgtaa gattccggtt cttgagacgg gggttcgatt ccctcaggac gcgttctact
     8881 gctattttag cataatttac gaatagttgc ttctttattt ttaagtgaaa actgtccgcc
     8941 aaagagaatt ggtggacagt tttttgttgt atctgtatag atat
//
LOCUS       DAZUMM010000093         8967 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000093 DAZUMM010000000
VERSION     DAZUMM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.096"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..1187
                     /gene="gmd"
                     /locus_tag="ACG8E6_002307"
     CDS             141..1187
                     /gene="gmd"
                     /locus_tag="ACG8E6_002307"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFS5062690.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
     gene            1195..2280
                     /locus_tag="ACG8E6_002308"
     CDS             1195..2280
                     /locus_tag="ACG8E6_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5062691.1"
                     /translation="MNKHICFYLSSHGFGHIARNLPIIMATLCRCEAKITLVCGANHL
                     DFAKRNLEQELPKELIDRVRYRPINTDIGLINREGTLLVDTGKLTEICEYFLAKVPSL
                     AKTEAEWLKREAVSAVLCDMPIWAMDACDMAGVPFLYVGNFTWTELYREFLPEHIWKE
                     YAKRYGKIQHTMLYALNNEEMLEFLQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVF
                     VALGMSARFKQPIDVSHAPYDFITTLGVPLVGENVTVISNSTPNTQDYIAAADYVITK
                     AGWGTVAECILAQRPMALFARDTVLEDRTTICKLQKLGLAISVTPENLHRVERVIEDL
                     KQLDTSNFDTFYDAASEIADKLISLMQ"
     gene            2317..3261
                     /locus_tag="ACG8E6_002309"
     CDS             2317..3261
                     /locus_tag="ACG8E6_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HFS5062692.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWENGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            3329..4642
                     /locus_tag="ACG8E6_002310"
     CDS             3329..4642
                     /locus_tag="ACG8E6_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HFS5062693.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVICASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            4834..5628
                     /locus_tag="ACG8E6_002311"
     CDS             4834..5628
                     /locus_tag="ACG8E6_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HFS5062694.1"
                     /translation="MTLTEVLQEHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPT
                     DEYAIEKENGQLGKTEMWYVLDASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKK
                     NDVFFIEAGTIHAIGAGALIAEIQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVAN
                     LKGLSEPKQPLRVLKYRAGVAAELLCRCKHFEVSRMIVNTERRQTVHYRADSISFRVL
                     LCVNGCGTVRFDNESLDIYKGDCLFVPADSTEISIHGSIQFLDVRG"
     gene            5633..7285
                     /locus_tag="ACG8E6_002312"
     CDS             5633..7285
                     /locus_tag="ACG8E6_002312"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFS5062695.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            7309..8175
                     /locus_tag="ACG8E6_002313"
     CDS             7309..8175
                     /locus_tag="ACG8E6_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS5062696.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(8217..8408)
                     /locus_tag="ACG8E6_002314"
     CDS             complement(8217..8408)
                     /locus_tag="ACG8E6_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062697.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCEKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            8567..>8967
                     /locus_tag="ACG8E6_002315"
     CDS             8567..>8967
                     /locus_tag="ACG8E6_002315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062698.1"
                     /translation="MEEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSN
                     LWFRQEISIAEIEDAVLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGTDVWKN
                     ISYLKVYTATPPKKPTPPRFQSNGWSLFFSH"
BASE COUNT         2432 a         1869 c         2273 g         2393 t
ORIGIN      
        1 cacgaaaaac taatattgtg ctaatgcgtg cgtagattgt tctatgaaat atcgaaaatt
       61 atcgtgtatt cttgaaaagt attagaaatt acgagccatt tatatggcct tatcacttac
      121 ataggaaagg ataatgtatc atgaaaaaag cgctgattac tggtattact ggtcaggacg
      181 gttcctatct ggcagatttc ctgctagaga agggctacga agttcacggt atcactcgcc
      241 gtgcttctat ttccaatacc gctcgcattg accatttgat gggcaagatc acccttcatg
      301 acggtgacct gtccgactca tcttctctga ttcgtatcat cagcattgtt cagcctgatg
      361 aaatctataa cctcgctgca cagagccatg ttcaggtttc ttttgatgtt cctgagtact
      421 ccggcgatgt tgatgctttg ggcgttatgc gtattctgga agcttgccgt attctgggcc
      481 tgacgaagaa gactaagatt tatcaggcat ctacttctga gctgtatggc aaggttgaag
      541 aagttcctca gcgtgaaacc actccgtttc atccttacag cccttatgct gttgcaaagc
      601 agtatggctt ctggatcact aaggaatatc gtgaagctta tggcatgttc gctgtcaacg
      661 gtattctgtt caaccatgaa tccgaacgtc gcggtgagaa tttcgtcacc cgtaagatca
      721 ccctcgctgc cggccgcatt gcagaaggcc tgcaggacca tctggaactg ggcaacatgg
      781 actccctgcg tgactggggc tatgcaaagg actatgttga gtgcatgtgg atgattctcc
      841 agcatgagac tcccgaggac ttcgtgatcg ctaccggcga gcagcacact gttcgtgact
      901 tcaccgagaa ggcattcgca gcaaatggca tgaccattcg ttgggaaggc actggcattg
      961 atgaaaaggg ctacgatgtt gaaaccggca agatgctcgt ttgtgtaaat ccacagtggt
     1021 tccgtccgac tgacgttgac aacctgtggg gcgacccgac taaggcaaag accgttctcg
     1081 gttggaatcc gcagaagacc tcttacgagg aactggttaa catcatggca aagcatgacc
     1141 gtgaattggc aaagcgcgaa gctgcaatga agaacgcagt caagtaagct gacattgaat
     1201 aaacatatct gtttctatct ttccagtcac ggcttcggac atatcgcgcg gaatctgccc
     1261 attattatgg caacactctg ccgatgcgaa gcgaagatta cactggtttg cggcgcaaat
     1321 catctggatt ttgcaaagcg caatttggag caggaactgc ccaaagagct gatagaccgc
     1381 gtgcgctatc gtccaataaa cacagatatc ggtctgatta accgtgaggg caccctactg
     1441 gtggacacgg gtaagctgac cgagatctgt gaatattttt tggcaaaggt tccttctttg
     1501 gcaaagacag aagccgaatg gctcaagcga gaagctgtca gcgcagtgct ttgcgatatg
     1561 ccaatctggg caatggatgc ctgcgatatg gcaggagtac ctttcctgta tgtaggaaac
     1621 ttcacttgga cagagctgta tcgcgaattt ttacccgaac atatttggaa agaatatgcc
     1681 aagcgttacg gtaaaattca gcacaccatg ctttatgcgc taaataacga agaaatgttg
     1741 gaatttctcc aaaacagcga aaaaacaccg acttccgttg tggcgcgtcc gattcataac
     1801 gacgaagtgc aacgcattaa atcacagcat gaaaaaccgc ctgtatttgt cgcactcggt
     1861 atgtccgcgc ggttcaaaca gccgattgat gtcagtcacg cgccgtatga tttcattaca
     1921 acgctaggtg ttccgcttgt gggagaaaac gtaaccgtta tttctaacag cacccccaat
     1981 acacaggact atatagcggc ggcagattac gttattacaa aggctggatg gggaactgtt
     2041 gcggagtgta ttctcgcaca gcgtccgatg gcactgttcg cccgcgatac agttttggaa
     2101 gatagaacaa ctatttgtaa attgcaaaaa ctgggtctgg caatttccgt cacaccggaa
     2161 aacctccacc gcgtagagcg ggtgattgag gacctcaaac agctagacac ctcaaatttt
     2221 gatacatttt atgatgctgc gagcgaaatc gcagacaaat tgatttctct catgcaatga
     2281 gagatgctct aaatttagaa aggagcaagc attattatga tggaaaaaga cgcaaaaatc
     2341 tacgtagccg gtcatcgtgg catggctggt tccgcaatcg tgcgcgagct gaaccgtcag
     2401 gactacaaca atattatcac ccgcacccat aaggagcttg acctgtgccg tcaggacgct
     2461 gttgaagcat ttttcgcaca ggaaaagccg gattatgtat ttctcgctgc tgcaaaggtt
     2521 ggcggtatta tcgcaaacca gaacgctctg gcagacttca tgtatgaaaa catgattctg
     2581 gaaatgaacg tcatcaactc cgcatgggag aatggctgta agaagttaca gttcctcggt
     2641 tcttcctgta tctatccgcg tatggcaccg cagccgatgc cggaaagctg tctgctgaca
     2701 agtgagctgg aaaagaccaa cgaggcttat gcactggcaa agatttctgg cctgaagtac
     2761 tgtgagtttc taaacaagca gtacggtact gactatatct ctgttatgcc tactaacctg
     2821 tatggaccta acgacaacta tcatcctact cacagtcatg tgctccccgc tttgattcgt
     2881 cgcttccatg aggctaagga agcaggtctg cctactgtta cctgctgggg tgatggttct
     2941 cctctgcgcg aattcctata tgttgacgat ctggcaaacc tgtgtgtgtt cctgatgaat
     3001 aactatagcg gtgacgagac tgttaatgca ggtactggca aggaactgtc tatcaaggaa
     3061 ctgaccgaga tggtagcaaa ggttattggt tacgaaggcg agattctgtg ggataccagc
     3121 aagccgaacg gtacacctcg taagctgctg gatgtttcca aggcaaccaa gctgggatgg
     3181 acatataaga ctgagttgga agatggcatc cgtctgtcct acgaggactt cctgaataac
     3241 ccgatgcgtg ctgaacgcta atttaccgag gttgctgtga tttttgagga ttgcagcagc
     3301 ctctttctta taaaaaggag agatcattat gaatattgtt ttactgtctg gcggttctgg
     3361 taagcgcctt tggccgctgt ctaatgacat ccgttccaag cagttcatta agattttcaa
     3421 aactgaagat ggatatgaat ccatggttca gcgtgtctac cgccagatta agaaggtaga
     3481 taacgacgca actgttacga ttgcaacctc taagacacag gtttctgcta tccataacca
     3541 acttggtgag gatgtgggtg tatctgtaga gccttgtcgc cgcgatacct tcccggcaat
     3601 cgcattggct accgcttatc tggtagatgt gatgggcatt gatccagaag agtccgtagt
     3661 tgtttgcccg gttgaccctt acgtagaaga tgactatttt gaggccctga aatctctgtc
     3721 cgatcaggct gacaaaggcg aagctaatct ggtgctcatg ggtattgaac cgacttatcc
     3781 ttccgagaag tacggctaca tcattcctga gagcgcagag cagactgctt tcgtaaatac
     3841 cttcaaggaa aaaccttctg aggctgtggc aaaggaatat atcgcacagg gtgcactgtg
     3901 gaacggtggt gtgtttgtct acaagctgaa gtatgtcatg gagcgtgcac atcagctgat
     3961 tgactttgta gattatcagg atctgttcaa caaatacgat actctgacta agatttcctt
     4021 cgactatgcc gttgttgagc atgaggacaa gattcaggta cagcgttttg ctggtatgtg
     4081 gaaggatctg gggacttgga atactctgac cgaaggtatg gaggaatcca ttatcggtaa
     4141 aggtgttatg aatgagaatt gcgatggcgt acacatcctg aacgaactgg atgttcctgt
     4201 gttggctatg ggtctgcatg atgtggtcat ctgtgcatct cgggaaggta ttctggtttc
     4261 cgataaggaa cagtccagct atattaagcc tttcgttgac aagttcgagc agcagatcat
     4321 gttcgcagag aagtcttggg gtagttataa ggttatggat gtagaagcag agagcatgac
     4381 cattaaggtt actctgaatg ctagtcactc catgaattat cacagccaca agaaccgtga
     4441 cgaggtgtgg gtggttctgg ccggtactgg taagaccatc gtggatggat atgagcaggt
     4501 tgttaaggct ggcgatgtga tcaccatgag tgctggctgt cggcatacca ttatggcaga
     4561 tactgagctg aagctgattg aagtacagct tggcaaggat atcaatgtta aagataaaca
     4621 gaaatttgag ttagagtatt aaaaaacgga ggagggctcg ttatacagaa accgttttta
     4681 ttgaagcctt ccgcaaagga ttatctgtgg gggggaaccg cctgaatgat gaatttgcaa
     4741 aaggaattga gatgtctcct cttgcggaaa cttggaatgc tctacgcacc cggatggacc
     4801 gagctttgtt gtgtctggtc agagtatcag gggatgacct tgacggaggt gttgcaagaa
     4861 cacccagagt ttctgggaac gcatccgcag accaaaggag aattgccgat cctgatcaaa
     4921 ttcattgatg cgaaaaagga tttgtctgtt caggtacatc ccacagatga atatgcaatt
     4981 gaaaaagaaa acgggcagct tggaaagaca gagatgtggt atgtactgga tgccagctta
     5041 atctatgggc tgcagaaaat aacagaagaa gctgtgatcc gtgaagcgat tgctcaggga
     5101 actgttgagc gttatcttca gaaggttccg gtcaaaaaga atgatgtgtt ctttattgag
     5161 gcgggaacga ttcatgcgat tggtgcgggt gcactaattg cagaaattca ggaaaattcc
     5221 aatctgactt atcgtctcta cgattatgac cgtgttgaca aaaatggtaa aaagagagaa
     5281 cttcatgtag acaaagcgct ggaagtagcg aacttgaaag ggttgtcaga accgaaacag
     5341 ccgcttcgtg ttctaaaata tcgtgctggt gtagctgcgg aattgctttg cagatgcaag
     5401 catttcgagg tctctcgtat gattgtcaac actgagagaa gacagacggt acactatcgt
     5461 gcggattcaa tcagcttccg tgtgttgctg tgtgtcaatg gctgtggtac cgttcgattt
     5521 gataatgaat cgttggacat ctataagggg gactgccttt ttgtgccggc cgattctact
     5581 gaaatcagca ttcacgggtc gattcagttt ctggatgtga gaggataata atatgaatta
     5641 taaagtagaa tatgagcgtt ggctgaatca tgccaatgca gatattatag aagaactgcg
     5701 ttcctattct gagaaggaca ttgaggactc tttctataaa aatttgtctt tcggtactgg
     5761 tggtctgcgt ggtaccatcg gtgcaggcac taatcgcatg aacgtacaca ccgtaggtaa
     5821 ggcaagccaa ggtctggcag actatctgaa taagaactat gaagccgctt ccgtggtggt
     5881 tggctatgat tcccgtatca aatctgatgt gtttgcaaag actgccgcag acatctttgc
     5941 tgcaaatggt attcaggtgc acttgtggcc agaactgctt cctgttccta cagtatcttt
     6001 tgcgactcgt tatctgaagg cagatgccgg tgttatgatt acggcatctc ataacccttc
     6061 taaatataac ggctacaagg tatacggtgc agacggctgt cagattacta ccgaggctgc
     6121 tactgcaatt ctggcagaaa tcgaaaagct ggacatcttt gctgatgtaa agatggatgg
     6181 cgatgcttct aagattgcat ccattcagtc ggaagttctg actgctttca tcgaagaagt
     6241 gaaggggcag tccgtcctgt ttggcgaaga agtggacaag aatgttgcta tcgtgtattc
     6301 tcctctgaat ggtaccggtc tggtgcctgt tacccgcacc ctggcagaga tgggatacag
     6361 caatgtgacc gttgtggaag aacagcgtct gccggacggt aatttcccaa cttgtcctta
     6421 tccgaaccct gaaattaagg aagctatgtc tctgggtatg gagtatgcca agaagtacaa
     6481 tgcggatctg ctgttggcca ctgaccctga ctgcgaccgt gttggtatcg cagttaagag
     6541 cgcacatggt gactatgttc tgttgtctgg taatgagact ggtctgctac ttctggacta
     6601 catctgttct cagagaatta agcatggaaa aatgcctgct gaccctgtga tggtcaagac
     6661 catcgtcacc atggatttgg gtgaacgtat cgcatccaac tatggtgttc gtaccatcga
     6721 tgtgctgacc ggctttaagt ttatcggtga gcagattggt atgttggaaa aggctggtaa
     6781 ggttgacagc tatatcttcg gctttgagga atcttacggc tatctgtccg gtagctatgt
     6841 ccgtgacaag gacggtgtta acgctgctta tctaatctgt gaaatgttct cttactacaa
     6901 gactcagggt atcagcctgc tggataagct gaacgagatt tatacaaagt atggctattg
     6961 tttgaacacc ctgcattcct tcgagtttga cggtgcggca ggttttacca agatgcagga
     7021 aattatggtt gaattccaca agggtctcga tataattggt ggtaagaagg ttattgaaac
     7081 tttagactac agtaagggcc tgaatggtct gccgaagtct gatgttttga agtttatgct
     7141 ggaagataac tgctctgttg tggttcgtcc ttctggtact gagccgaagc tgaagaccta
     7201 tatcagcgtc agcgcagaga acaaggaagc cgccgaagct atggaagcta ttatggctgc
     7261 tgaactggcg gaaatgttcg agtaagaaaa gaaaggagaa atcttgtaat ggaggaactg
     7321 ttttctaacg ctttgaaatc ggtgaagtca aatcgtgtgc tgtacacggc atcgttcttt
     7381 gcaagatcct ctcttttgta tttgcaggaa attggacagc tgactgctct gaaggcacat
     7441 acatctaggc gtacaggatt gtcatcttac ctttgtttcc tggtagaagt tggttcaggc
     7501 acattggagt ataaggggca gaaatatacg cttcaagtag gagatatggt gtttattgac
     7561 tgtttgaagc cgtatgtcca tgagtcatca gaagatgatt tgtggacttt gcggtggtgc
     7621 cattttaatg gcattggtat gacggaagtc tacaaaaaat accttgaacg aggtggtcaa
     7681 ccggtcttta aaacatcaaa aacagataag tatgctgact tgctgaggag ccttcatatg
     7741 gtggcagagg gacaggagta tgtccgggat atgaaaatca atgttctact cagtgaactg
     7801 ctgatgcatt tgatggaaga ttcttggaat cctgacaaag tgccgaattt ctctacaaaa
     7861 aaaatggaat tgcagaacat aaaacaatat ctggatgacc attacacgga gaagattacg
     7921 ctggatcagt tgagtgcaga gttttttatc aataaacagt acttagctaa gatttttcgt
     7981 gaaacttacg gtacaactgt tgtcggttat ctgaatcaga aacgaatcac acaggccaag
     8041 cacttgctac gctttacaaa tctaagtatg gaagagattg caacaaaaat tggcataaca
     8101 gatgcaaact atttttccag aacttttttc aaggtggaag gtgttcgtcc cagcgaattc
     8161 cgcaagatgt ggtaataaaa agccggcggt gttgagccgc cggtcagtac ctgtcgttaa
     8221 cccgcattca aagctttaat ctcagctttg atttcagcac gaacacgata gtaatcttca
     8281 gtttcgattt cggtatccag ccgagttgct gtcttttcgc agtcaggaca acgctctggc
     8341 tgttcgtcgg cagggaagct gtagtggcag gaatcacagg tgtaaatgtg atcgtcacgt
     8401 ataatcatca cctaccaatc cagtgcttag atttttcatg caattactat tatatcgaat
     8461 tggtgattcc acaaaaatcc gcttcttgat aaatgtacga ttcagagtga tatatagtac
     8521 tgtactgcca aagggaatac cacaacggca gaaatggatg attcacatgg aagaaattat
     8581 gcagatgatt ttagggaacg caaaattcca gctcggacag gtggtttcga cccctgctgt
     8641 attggaaact ttcaaccccc tgcagaccgc aaacggcatt cccgaagcct tgagcaacct
     8701 ttggtttaga caggaaatta gtattgcgga gatcgaggac gcagttctgg atgccaagaa
     8761 cggcaaggag ctagcaaaca gcctcaaccg catcaagctt ttccgcaagt tcaccctcga
     8821 ccgagagctg gacggcatgg tcagactgaa gggcacggac gtctggaaga acatcagcta
     8881 cctcaaggtc tacaccgcca cgccgcctaa gaaaccgacc cccccaaggt tccagtcgaa
     8941 cggctggagc cttttcttct ctcataa
//
LOCUS       DAZUMM010000094         8964 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000094 DAZUMM010000000
VERSION     DAZUMM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.097"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..900
                     /gene="purR"
                     /locus_tag="ACG8E6_002316"
     CDS             82..900
                     /gene="purR"
                     /locus_tag="ACG8E6_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFS5062699.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1403..2776
                     /gene="glmU"
                     /locus_tag="ACG8E6_002317"
     CDS             1403..2776
                     /gene="glmU"
                     /locus_tag="ACG8E6_002317"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFS5062700.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2870..3841
                     /locus_tag="ACG8E6_002318"
     CDS             2870..3841
                     /locus_tag="ACG8E6_002318"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS5062701.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3847..3949
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4015..4584
                     /gene="thiT"
                     /locus_tag="ACG8E6_002319"
     CDS             4015..4584
                     /gene="thiT"
                     /locus_tag="ACG8E6_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFS5062702.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4665..5438)
                     /locus_tag="ACG8E6_002320"
     CDS             complement(4665..5438)
                     /locus_tag="ACG8E6_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062703.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5705..7405
                     /locus_tag="ACG8E6_002321"
     CDS             5705..7405
                     /locus_tag="ACG8E6_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFS5062704.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7375..7593
                     /locus_tag="ACG8E6_002322"
     CDS             7375..7593
                     /locus_tag="ACG8E6_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFS5062705.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7613..8503
                     /locus_tag="ACG8E6_002323"
     CDS             7613..8503
                     /locus_tag="ACG8E6_002323"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFS5062706.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8521..8805
                     /locus_tag="ACG8E6_002324"
     CDS             8521..8805
                     /locus_tag="ACG8E6_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062707.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         3024 a         1533 c         1887 g         2520 t
ORIGIN      
        1 tttttcataa aatgctcgaa tttttgaaga aaaacttata taatgtaaaa cgataaaatt
       61 tcttaaaagg agttggaata ggtgaaagta cgtcgcagtg aacgtctagt tgacatgaca
      121 cagtatttat tgaatcaccc gcatgaattg atttccttaa caagctttgc cgaacgttat
      181 gaatctgcta aatcttctat cagtgaagat ttgggaatca tcaagaaaac atttaaagaa
      241 cgagggtatg gaaccttgga aacaatcccg ggtgctgcag gtggtgtatt gttcatccct
      301 gaaatttcat atgaagaggc gaaaaaatat attgaatctc tagcagaacg tctatcagaa
      361 caagaccgtc tgcttccagg aggatatgtt tatctctctg atttgttagg agaacctgaa
      421 ctgctgcgtc aagttggaag aatcattgct tctaaatacc ttggtgaaaa aatcgatgct
      481 gtaatgactg tagccacaaa aggcgtacct attgcccaag ctgtgtccta ttatctgaat
      541 gcgccattcg ttattgtacg ccgggactca aaaataacag aaggatcaac agtaagtgtc
      601 aactatgtgt ctggatcttc tgaaagaatt gaaaagatgg aactgtctaa gagaagtttg
      661 aaacgtggtt cacgtgtcct tgtagtggat gatttcatga aaggcggcgg aacagtcaac
      721 ggaatgaaga gcttgattga agaattcgaa tcagaacttg ttggcatcac tgtatttgcc
      781 gaatcaaaat tcaacggtca tcgcgccatc gatgattata cgtcactgct ttatgtcaaa
      841 gatgtagata ctcaaacgaa aaatattacg gttgtgccag gaaattattt ttcagaataa
      901 gaggttgtga aaaagctgct tagtttcgag tagtaagaaa aattttgtga aaatggcttt
      961 ccacattttt tcataaattt ggcttaatac cgagaaactg gcttttgaac accgtttatc
     1021 tagaggttgt gaaatcagcg tcctgtcctg agtattagct caataaacgg aaaaattgct
     1081 cctcatattt tgccgtttat tgagctaata ccgaaggtca gctgattgaa aaacgtttaa
     1141 tgaagaggtt gtaagcctac cgtttaactc taagtcacaa gaaacgattt caaaaaacag
     1201 cttttcatgt tttatgagta gtgtgacttg tgaccgagga gttggcagga gagcaccggt
     1261 taccaaatag ataggcccgt tatggtctat ctattttttt atccagaaaa cgctgggata
     1321 aaagaaaatc acttgttttg ttgaaaaaat gggtatactg ggataaactg aaatcagcaa
     1381 actatattta ggagtgacgc agttggacgc acgttatgca attattttgg cggcagggaa
     1441 aggtacccgc atgaagtcaa aattatataa agttttacat cccgtttctg gtaaacctat
     1501 ggtagaacat atcattaatc gggtcagtga aacgaaacca gatgaagtga ttacgatcgt
     1561 tggacacgga gcagaacaag ttaaagcaca actaggagaa cgcagtaaat atgcgttaca
     1621 agcagaacaa ttgggaacag gacatgctgt tttgcaagct gcttctttct tggaaggaaa
     1681 aaaaggaaca acgttagtta tcagtggcga tacaccatta ttgactactg aaacgctgaa
     1741 caatcttttt gaatatcacc aaggaaaaaa tgcaagtgca acgatcttga cggcacaagc
     1801 agaaaaccca acaggatatg gacgaatcat ccgtgaccat attgggatcg tagaaaaaat
     1861 cgtggaacaa aaagatgcaa caccagaaga agctcttgta caagaaatca atacaggaac
     1921 ttactgtttt gacaacgaag ctttgttcga tgcgttaagc aaagttggaa caaacaatgc
     1981 tcaaggtgaa tactatctaa cagatatcat cgaaatctta aaagaagaag gccatacagt
     2041 tgctgcttat caaacggatg attttgaaga atcaatggga gtcaatgatc ggatcgcgtt
     2101 agctaaagca aatgagatca tgagaaagcg aattaaccag atgcatatgg tcaatggcgt
     2161 aagctttgtc gattcagcaa cgacatacat cgatgcaggc gtagaaatcg gtccagacac
     2221 attgatcgaa gcaggtgtac aaatccaagg aaatacagtc attggttctg attgtgtcat
     2281 cgggtcacat tcaaaaatcg tagacagtag gattgaggat cacgtcgtca ttgaaaactc
     2341 agtcattgaa agcagccatg tcaagaaaca tgcagatgtg ggaccttatg cacatttacg
     2401 accaaaagct gaaattggcg aaaacgtcca tatcggaaac tttgtggaag tgaaaaatgc
     2461 tcaaatcggc aaaggaacta aagtcggtca cttgacttat gttggtgatg ctacgctggg
     2521 agaagaaatc aatgtcggat gtggtgttgt cttcgtcaac tatgatggaa agaacaaaca
     2581 tcatacgact gtcggcgacc acagctttat tggttctagc acgaatatca tcggacctgt
     2641 cgaggtagct aaaaacagtt cgattgctgc cgggtcaaca attacagaca atattccaga
     2701 atatgcgctg gcaattgcga gagcaagaca agtcaataaa gaaggatatg caaaaaaact
     2761 cccttatcta aactaagact tttgcaaaat cacttgattt atttaaaaaa tatgactact
     2821 atttattagg tagaattcta gagttgtgaa aaagaaagtg gaggtcccca tgtcaaaaca
     2881 ttactttgac ccaagattaa aaatttttgc gttaaattcc aatcgcccat tagcagagaa
     2941 aattgcagca gcagtcggcg tcgagttagg aaaatcatct gttactcaat ttagtgatgg
     3001 agaaattcaa gtaaacattg aagaaagtat tcgtggatcg catgtctatg ttattcaatc
     3061 aacgagcagc ccagtcaatg ataacttgat ggagttgctg atcatgattg acgcactgaa
     3121 acgagcaagt gcaaaaacaa tcaacgtagt catgccttat tatggctatg cgcgtcaaga
     3181 ccgtaaagca cgtgcacggg aaccaatcac agcgaaatta gtagcaaaca tgattcaaaa
     3241 agctggtgca acaagaatgc tgactttgga ccttcatgca gttcaaatcc aaggtttctt
     3301 tgatattcca gtcgatcatt tgatgggtgc gccacttatt gccaattatt tcttagaaaa
     3361 aggaatcaaa ggcgatgatg ttgtcgttgt ttcaccagac catggtggtg tcacacgggc
     3421 acgtaaatta gcggaatttt tgaaatctcc gattgcgatc atagacaaac gccgtccaaa
     3481 agcaaatgtt gcagaagtaa tgaatatcat tggacaagtc aaaggcaaaa cttgtgtact
     3541 gatcgatgat atgatcgata cagcaggtac gatcacattg gctgccaatg ctttgaaaga
     3601 agctggtgca actagtgtgt atgcttcatg tacacatcca gtattatccg gccctgcttt
     3661 acaaagaata gaagattcag ctatcgagcg tctagttgtt acagactcga tctatcttcc
     3721 agaagatcgt aaaatcgaca aaatcgatga agtaagtgtt ggcggactaa tgggagatgc
     3781 aatcaaacgt atccatgaaa acaaaccagt tagtccattg tttgaaacaa aaaataaata
     3841 ataaataaac tcacaaaggg gagtccaatt gggctgagat tgaatctatt tctaaaccct
     3901 tcgtacctgt atcggttatg cgagcgtagg aattgtgaat aaacagctgt ttgactattt
     3961 tcgaaagctg cttcctcctt tgtgactttt ttattcaaag gaggaagttt ttttatggga
     4021 aaaaatagag tttgggtaga aggaacgatt gttgcagcgt tagcgatggt gttgtcgttg
     4081 attccgatcc agatcggcag cagtttttct atttcattag ggcagattcc attgactctt
     4141 tttgcgttaa gaagagggac aaaggcaggg atgctggcag gtttcatttg gggcatccta
     4201 cattttccgt tgggacaagt ttattattta agtgtcgttc aagtgttgat tgagtacccg
     4261 attgcctata cttttgcagg cgcagcaggg ctcatggctt caaatgtcca aaaaggctat
     4321 gtagacgata atacgaaaca aattagaaaa agcatcgtgt ttggtgcatt gattggcgca
     4381 ctttccagat atttttggca ttttattgca ggagtggttt tctggggagc gtatgcacta
     4441 tgggggatga atccttggct cttttctttc gtaatgaatg gtgccagcgg gttgagtaca
     4501 gctattgtaa cgatcatcgt tttattaggg atccaacaaa cgactccttc gatttttgtg
     4561 ccagataaac gacaggagct gtaaataaaa aagaagcaag aaacttgaga cagaatcatg
     4621 tcctagtttc ttgtttcctt tttatttttt gataagaaat agatctactt caatggcaga
     4681 taagaaagat cggcatcttc taccaattgc cagtgtcgat cggtgacaga gtagattaaa
     4741 gtagcgatga tttcctcttc tcgataccgg gtgcctttta atttccaaat ataatcttca
     4801 atcacataga ttgggatacg atatttacca tcgatcaaac caacttgatc aaaagaaaaa
     4861 atgatccctt cctgcatcaa attacgcatt tctttcaata attccacact gattttttca
     4921 acaggtgttt cttttttgaa aaaagatttt tttacattga tcttatctga caagatcgaa
     4981 cttaatttac tagcaaaaat cctataaaat tcatctaaat agcgataata aatccgattc
     5041 agctgatcat taagatcgaa gtatacaaaa ttattctgta gtttataaaa aaacggcgaa
     5101 tgaagatgtg ttttcatatg accaaaatat aataattctg aaatttctaa aggtgtcaat
     5161 tctttcagca tcgttaaatc agtaaagtcg atccacttca attcatcgaa tccacgcctg
     5221 ttacgagaag aggaaaagaa acgctgaata ttttctgtac cacggattac tttcagccct
     5281 gtatgggatt catattctcc agcagaagca gatggatcaa gcaagagcaa gttttgagga
     5341 aaatgcacga tactgtttgc aaagtcagta gcagtcagcc cttttgttaa aacagcatta
     5401 cttgtggtat caatatgtac ataaactgac tcagtcatca tgttcctcct cttatttcct
     5461 cttaatctct tttattttac aacatttgca ttaaaaaagc ttgtttcttt tattttaaaa
     5521 ttccattaca aaatcacgtt ttcattgaaa acactctcat tttcaattaa tttttgtgat
     5581 ttttgtattt ttttatttaa aggaggtttt atgaattgtt ttcttatgga atctacaaag
     5641 tcgaatacga gactcatgac ctttttatga cgtactggta tacttgaaga aaaaggagag
     5701 cgcgatggag ataaaaaaag tttacttttc acagaaaatg tcgctgtatt cgcctatcca
     5761 gcaagcaaaa caaccccttc cattttttca aagtcatcaa gaaagtagag acgctttttg
     5821 gcgttctgca aaaaagcaat gtgcttttaa agcagtatcg aaaaaatata ccgtctattt
     5881 tactattgct aaaagtagag gagatgttat ttttaataat ctgttgattg aaggtgaaac
     5941 ttttgaatgg aaaaaatttt ttcggtatat ggaagcttgc gctcgttttt ttattaaaga
     6001 aaactgttgt tttttatttc aatctcctct atcagaagaa tggttgcgta tttttcataa
     6061 aaacggctat caaggaacca cacaattaaa taaaaagctg gtctatcata cagcacttgt
     6121 tttaggtgga ggaggagcgc acggtgctta tcagattggt gtgtggcagg cgctgaagga
     6181 acatgatatc aactttgaga ttatcacagg tacctcggta ggtgcgttga acggcgcatt
     6241 gattttacaa ggagatatga aaaaagctgt gaatttatgg aaaaagctgt cgacaagaca
     6301 agtgcttgcg ttaccagaaa tggctgcagt tgaagacctt cgtgaacgct tctatcgaga
     6361 aagacgccaa atgacgaaaa cagcactaat tgaaggcggg gtttcttctg ctccattgga
     6421 aaagatggtc aaagacaatt tggatttgtc cttactcaaa cataatccca aaatacgttt
     6481 atatacggtg tctactaaac tgccagaact agcggaagta gtgactgata tccaacagac
     6541 aaaaacagaa gaaataccag attggatact cgcctctgct tcattttatc ccgctatggc
     6601 ttatcgaaag atcggaaaaa ataaatatgc agacggtggg tatcgcaaca aaatacctat
     6661 tgatattgcg attaacaaag gagcaacaga ggcatttgtc gtagatgtgc aagggccagg
     6721 cccagcgaaa aaaattcgta tgccagacat ttttatccat tggaaatgtc aaacattgtg
     6781 gacactaggc agctttctgc tttttgatag ccaaaggaac caattaaatt tacaattagg
     6841 ctatctggaa atgaaaaaac gattaggcta ttactatggg aactggtata catttcattc
     6901 tgttaaacct gcaagaacat actggcgagg atttttgtct tacttaacaa aagaaattca
     6961 gttagaatta tcttttttca aaagcataaa attttggcaa aaactgcgta atttatataa
     7021 gaacagggtc gtaccagaga catgcggatt ggcgatgcta gaacttttag caaaaaaaca
     7081 ttttttactg cccaatgaaa tctatcaagt agacatgatg attcaaatga tctgccaaca
     7141 agatataaaa agtatgccgg atgatttatt ggcacaaatt ggtcaattaa gtactgaaga
     7201 atggcgaagg tatcgaaaat accagcaaaa aatacaaaat gagcgcacga aaactgttta
     7261 ttttgattat ctgctacaag gaaaacgcaa ggatcgtttg cagcatgaat tgcttaagca
     7321 accagttgac acattactga tcctatattt atattatctc aaggaggaac aaccatggca
     7381 caagaatttt catatgaaat cgtagaagaa atcgctatat tgtcagaaaa caacaagggg
     7441 tggcgtaaag aactgaacct agtcagctgg aatggtcgcc cgccaaaatt cgatcttcga
     7501 gattgggcgc cagaccatga aaaaatgggg aaaggcctga cattgacgaa cgaagagttt
     7561 gaacaactgc aaaaagcaat agaaaatatg taatgaggaa ggaccctgtc gaatgaaaag
     7621 tatgactggt tttggcaaag ccatccgcga aacaaaagcg tatcagttag atattgaaat
     7681 aaaaagtgtc aaccagcgtt ttctagatgt tcaaatacga agtcctaaaa tcctgaactt
     7741 tgttgaaaac gattttcgtc agcttattaa acagcagtta agccgcggtc gaatcgaagt
     7801 atttgtgaat ctagcttata ttggcaaaaa cgaaaaacag atcgtcatca actgggaatt
     7861 gattgattca cttatcactc aattgacaga cggaattcaa caacgatacg gtagtcaaaa
     7921 tcaagtgtca attagtcgga tggcggaacg cttcgcgctg gacgaaaact tcgtaacggt
     7981 agaagaaaaa ccggaagaga gcatggaaga attggcacag ttagctctag atacattgaa
     8041 aagcgcactt gattctattg aggcgagtcg agaaaaagaa ggtcaagctt tagctttagt
     8101 gttgaaaaag aatactgaag aattcaaaga agtactaaaa aaactcgata gttttgtaga
     8161 aatctatgaa aaggaatttc aaataaggta tcaaaaaaag ctggaggaat ttcttggagc
     8221 gaaagtcgat gagcaacggt tgttgacgga gctagccatc ttgttggaac gcggagatat
     8281 tcacgaggaa ctggatcgtc tggcgatcca tgtacaaaaa ctagatgaac ttatcgaagc
     8341 acaatgtcct gtaggtcggg aattagattt cctgatccaa gaaatgaatc gggaaatcaa
     8401 cacgatcggt tccaaatcaa atgcaatcga aattaaaaat caagtcgtcc agtctaagac
     8461 gatcttagaa aaaatcagag aacaagtaca aaatattgaa tgacgaaaga ggtagagata
     8521 atgaaatatt atgacgtaac gttccacgaa ttaagtggaa aaactgtggt taaacgggat
     8581 atcccttctg aaaaaaacgg ctttgacgta tggaaagatg cctgtgcaga ctataatgag
     8641 aacgaactgt ttattttgat caacgacggt gcttacgtga cgatgaatcg gaaatttatt
     8701 gttcgaattg acacagaaga ggtagaagat ccgacagaaa aagcacgttc tcgaaaagat
     8761 gagatcatgg gtgtagtgaa tactttgtcc aatatgggct tttagcaagg atgctgaaca
     8821 aagaaataca ataaataatc agaattggat ttcttcgctt agcttctttg ctctaggaat
     8881 acatttaaag gttgtgacaa aattcgtgtc acaatctttt tttattttga ataagtgatg
     8941 ttcaaaaacc attttctcgg tatt
//
LOCUS       DAZUMM010000095         8681 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000095 DAZUMM010000000
VERSION     DAZUMM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.098"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..74
                     /locus_tag="ACG8E6_002325"
     tRNA            2..74
                     /locus_tag="ACG8E6_002325"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ACG8E6_002326"
     tRNA            78..150
                     /locus_tag="ACG8E6_002326"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ACG8E6_002327"
     tRNA            155..225
                     /locus_tag="ACG8E6_002327"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ACG8E6_002328"
     tRNA            238..311
                     /locus_tag="ACG8E6_002328"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ACG8E6_002329"
     tRNA            323..394
                     /locus_tag="ACG8E6_002329"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /gene="metK"
                     /locus_tag="ACG8E6_002330"
     CDS             660..1853
                     /gene="metK"
                     /locus_tag="ACG8E6_002330"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFS5062708.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ACG8E6_002331"
     CDS             1983..3455
                     /locus_tag="ACG8E6_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS5062709.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ACG8E6_002332"
     CDS             3538..4326
                     /locus_tag="ACG8E6_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5062710.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ACG8E6_002333"
     CDS             complement(4393..5061)
                     /locus_tag="ACG8E6_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFS5062711.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ACG8E6_002334"
     CDS             5176..6888
                     /locus_tag="ACG8E6_002334"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5062712.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="ACG8E6_002335"
     CDS             6891..8663
                     /locus_tag="ACG8E6_002335"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5062713.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2612 a         1484 c         1933 g         2652 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt a
//
LOCUS       DAZUMM010000096         8531 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000096 DAZUMM010000000
VERSION     DAZUMM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.099"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..913
                     /gene="rsmI"
                     /locus_tag="ACG8E6_002336"
     CDS             41..913
                     /gene="rsmI"
                     /locus_tag="ACG8E6_002336"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFS5062714.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            1022..2143
                     /gene="dinB"
                     /locus_tag="ACG8E6_002337"
     CDS             1022..2143
                     /gene="dinB"
                     /locus_tag="ACG8E6_002337"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFS5062715.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(2197..2799)
                     /gene="nrdG"
                     /locus_tag="ACG8E6_002338"
     CDS             complement(2197..2799)
                     /gene="nrdG"
                     /locus_tag="ACG8E6_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFS5062716.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(3114..5303)
                     /gene="nrdD"
                     /locus_tag="ACG8E6_002339"
     CDS             complement(3114..5303)
                     /gene="nrdD"
                     /locus_tag="ACG8E6_002339"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFS5062717.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIAEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            5629..6396
                     /locus_tag="ACG8E6_002340"
     CDS             5629..6396
                     /locus_tag="ACG8E6_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5062718.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            6386..8428
                     /locus_tag="ACG8E6_002341"
     CDS             6386..8428
                     /locus_tag="ACG8E6_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5062719.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         2632 a         1502 c         1703 g         2694 t
ORIGIN      
        1 aaatactgct ccagtctttt tattaaggag attgtgacag atgtataatc aaaaaagttt
       61 caagggaaat ccggtatatg gggatttata tcttgtaccg acgccaattg gaaatttaga
      121 agatatgagc tacagaagtg ttcgtatgct gcaagaatcc gatcttatcg caagtgagga
      181 tacgcgtaat acacaaaagt tattgaatca ttttgaaatc catacacccc aaaaaagttt
      241 acatgaacat aattataaag aacgtgtccc tcaattgatt gctttgttaa aagaaggaaa
      301 aacgattgct caagtcagtg acgcaggaat gccttccatc agcgatcctg gacatgagct
      361 agttttagcc tgcataaaag aagggatttc tgtgatctct atcccaggac ctacagctgg
      421 gatgacggct ttgattgctt cagggctagt gcctcaacca tttttatttt atggcttcct
      481 tccacgtaag aaaaaagaac agaaagaaac attgaaaaag ctaaaagaac agacagttac
      541 gctgattttt tatgagtcac cttatcggat cactgctaca ttgacgaatt tcttagaaat
      601 ttttggaaat cgccaagtcg ttttgtgtcg ggaattgaca aaggtccatg aagaatacct
      661 tagaggaagt acggaagagc tactcgctta tatcgaagaa catccagtaa agggtgaatg
      721 ttgtctgctt gtagaaggga atttgttcga aatgccagtg acggaagttg atgaagagtt
      781 gggaacgtta aaggaacaag tagaagcaaa gatcgcttca ggcgaaaaac caaatgctgc
      841 tatcaaagca gtagcgctga aaaatgggct gaaaaaacaa gaagtctacc gtcagtacca
      901 tgaattagac tgagatagta taagttgtct ataatgaaag actaatgagg aaatcagcta
      961 ctaacttttc aaatctcttg aacgcgaacg aatgttcttg tataatgaat aagaggtgag
     1021 gatgatggag ctgcaatttc ctttacataa tgatacatca agaaaaatca ttcatattga
     1081 tatggatgct tttttcgctt cggttgaaga acgagatcat cctgagtttc gcggacatcc
     1141 attagtaatt gccagacacc ccagtgacac tggtgggcgt ggagtagtga ctacagcaaa
     1201 ttatgaagca agaaaattcg gaattcattc agcaatgagt gcacaaaaag cgtatgagct
     1261 ttgtccgcaa gccatcttta agcctggcga ccatcataaa tacagcgata tttccaaaca
     1321 agtacgggaa gtattctatc gttatacaga tattgtggaa ccagttagta tcgatgaagc
     1381 gtatcttgat gtgaccgaaa ataaaatcaa ttgcaagtca gccatcaaaa tcgcccgaat
     1441 gatccaagca gatatttggg agagtgtcca gctaacatgt tctgcgggag taagttacaa
     1501 taagtttttg gccaaattag catcagacta tcaaaaacct agaggaatca cggtagttgc
     1561 ccctcaagac gctgtgccat ttttaaaagc tttgccaatt gaaaaatttc atggggtagg
     1621 gaagaaaact gtacctagaa tgcatgagct agggatttat acaggaaaag acctatatga
     1681 atgcacagaa atgatgttga ttcgaaattt tggaaaaatg ggctattctc tttatcgtaa
     1741 agtcagagga atccatgatt ctccagtaaa cgttacaaga gagcgaaaat ctgtgggaaa
     1801 agagcatact tatgggcagc cgttgcaaac ggaagaagca gtgctgactc agttgcgcca
     1861 actagctgaa aaagtagaag aagcactgag aagagtccaa aaacatggga aaacagtagt
     1921 tttgaaggtc aggtacacgg actattctac tgtgaccaaa agagtgacat tgccagaata
     1981 tatctataaa aaagaagcac tcttttatca agcaagcctt atatgggagg aaatcttggg
     2041 agtagaaaaa ggaatccgat tgttagggat tactttgacg aatctggacc cgatgactta
     2101 tgaaaacatc gtccttccat tatgggaaaa tcaagaaata taaaaacagc tttgagagtt
     2161 gaaaccggaa atccgttttc tctcccaaag ctgtttttat atcaagtctt gctttttgat
     2221 ttgttcatag gtttctgttg catccgtaca tttttcccaa atcacaactt gattggattc
     2281 taaagatttt tggacgtcaa tgatccgctg gttgctactt ccgcgaaatt gtagttttag
     2341 gtcacgtttg cttaattcaa agcgtccgtc aacaaggata tcgatttgcg aaaggagttc
     2401 taatttatct tctgaatctt gtagcaattc atcaaaggta tagcctgtcc atgaccagat
     2461 atcttttgtc gtaccgaact cttttcggat acgtcgtacg acttgcagac aaacatctgt
     2521 attcaaaaaa ggttcgccac caagtaaagt taatccttgc acgtaatcgt tgcgcaagtc
     2581 agcgatgatt tgttcttcta attcaggtgt gaatggtttg ccatatcgga aattttgtgc
     2641 agctttgttg aagcagcctt cgcaggcaaa taagcagcca ctaacgtaaa gactgttgcg
     2701 gacaccttca ccatcgacaa aattaaacgg tttgtagtca gcaatatagt tttggctgta
     2761 gtcttttgct aaccattctt ttggttttgg atttctcata aaaaaacttc tttcatttaa
     2821 ggttcaaaga tatagctatt gaagataaaa tctactcgac cacataaata agaacaaact
     2881 gacctaaatc cattgttcaa gaaagcttgt tcttattttg atagagggcg ttcaaagata
     2941 caaagccatc gaaagagggt ggtgaaaaaa gacttgtcat agcctcttat tctgaataaa
     3001 cggtgttcaa aagcttgcat ctgcagtaat aagcccaact aaacgaaaaa tatgaggaac
     3061 tatttttgtt tagctattct tattacttga tgcaggacag ctttttcaca acctcatttc
     3121 atgtgtttca cacgtgagga aatttcttta tggcggccat gtaccatcgg acgagcttgc
     3181 ggatttccta aatagccaca agtacgtttg actacatcac atgtttttgg atcatgattt
     3241 ccacattctg gacattcgaa ccctcgttct gtcggtttga aatcaccttc gaaaccacat
     3301 tgatagcaat gatcgattgg cgtattcgta ccaagatacc cgattttgtc atatgcaaaa
     3361 tcccatacag cttctaaagc tttcggattt tgttggagca tagggtattc acagtagtgg
     3421 atgaatccac ctgaacaata ttttggataa tccttctcaa attccaattt ttcaaatggc
     3481 gttgggtttt tacgcacatc atagtggaaa ctgtttgtat aatattcttt atctgtgata
     3541 ttttcgatga tgccgaattt ttctgtatcc aaacggcaga aacggtcagt tagactttca
     3601 cttggagttg aatagacact gaaatgataa ccatattcat ccccccatgc atctgcatta
     3661 gcttttaatt ctttgacgat atcaagtgtg aaagcttttg cttctgggtt tgtttcccat
     3721 tcgccaccga agaaagctga agcaacttca tacaatccaa tgtagcctaa cgatacagta
     3781 gctcgacggt ttttgaataa ttcattgaca gaatcggtac gtgaaagacg tttcccgaaa
     3841 gcaccataca tatataaaat tggcgcattt gctggaatgg cttctttaca acgttctaca
     3901 cgatagagaa gcgcatcttt catgatttct aagcggtcag acagtaactg ccagaattta
     3961 ttctgatctc cttttgattc gatcgcgatt cttggcaaat tcaatgtaac aacacctaaa
     4021 ttcattcgtc cgacatttac ttcttgacca ttttcgtctt tccacccttg caagaatgaa
     4081 cgacagccca taggtacttt gaaacttcca gttaattcaa cgattttatc gtaattcaga
     4141 atatcaggat acattctttt cgttgcacat tccaatgcta gttgtttgat gtcgtaatta
     4201 ggatcttcag ggtttaagtt cacacctttt ttcaaagtga agatcagttt agggaagata
     4261 gctgtccgct tttcactccc taatccattg attcgaatct gtaaaattgc tttttgaatt
     4321 tctctttcaa accagttttc tcctagtcca aatcctaatg aagtgaaagg agtttgtcca
     4381 tttgaagtaa ataaggtatt gatttcatat tccaaacttt gcatagcatc gaagatatct
     4441 tttttggttt ttgcttttgc atattctttt tggcgttctg gcgaatcgat ccattcttcc
     4501 gcgtctttta gatgtttttt atagttcaat tctgcaaaag gagcaagcaa ttcatctgtt
     4561 cggtcggcag aacagccacc atattgactt gacgctacat tggcaataat ctgtgagatt
     4621 tgtgctgtgg cggtctggat agattttggc gattcgactt cggcattgcc gattttgaac
     4681 ccattgttca gcatgccttt gaaatcaatc aagcagcaat tcgtcatcgg tgtataagga
     4741 tgataatcca aatcatggta atggatatcg cctttttgat gcgcatttgc tatatgtggt
     4801 ggcaacattt tcagtccgat tgattttcca acgatcccgg ctgtcagatc tcgctgtgta
     4861 ttgaaaacat tactgtcttt atttgcgttt tcgttcacga cagtttggtc cttgttgatt
     4921 agcttttcaa tcgaaaagtt gatatctgtt gctttactgc gagcaaaatc tcgtttggta
     4981 cgataattga tgtactcttc tgctaattca tattcgtttt tttctagtaa tatatgttcc
     5041 gcgatgtttt gaatctcata gattttgaca ttttcagcaa accggctttt gatttcttgg
     5101 acaaccaagt ctgcaatcat ttgaattttt tcataagtaa gtggtgataa tgagccgtga
     5161 attttctttt ctgctttgat cagtgcttca ttgatctttt gttcatcgaa atcaacatgg
     5221 cggccatcgc gtttgatgac tttcatctgg gatgtctctg gctgctgagt aacgacagtt
     5281 ttttcttcat aatttacttt cattatcttc aactcctgct aaattgttct tgtctatcat
     5341 aaaacaaaat cggggaagga gcaactaaat atcggctata tatagtattc aatgtgctgt
     5401 ataattggat ttctatacta tatgtagtgt atattttatt gtgaaaaaag gtgaaaactt
     5461 gtagctggtg gatttgtttt ttgcatattt aattttgctg aataaaataa ttgaataaaa
     5521 ttttttattc agtccaagta aaagtgacaa aaacataaga gcaaattatt cgtagatatg
     5581 ctactatgca aataaataga aatgaaaaat gaaagaggag gagaagaatt ggaaaagatg
     5641 atcgaagtga aacatctaag taaaagctac ggcagcaaac tttctcccgt caaagcctta
     5701 gataatatct cattcacagt agaagaaggc gagtttgttg ggattatggg cccaagtggt
     5761 gcaggaaaga caacgctgat gaatatcttg tccacgatca atttgcctac gatgggcagt
     5821 gtctcgatcc aaggaaaaga aatcacaaaa atgaaaaaca gtgagctcag tgactttcgt
     5881 cgaaaaaaac taggattcat ttttcaagag ttcaatttga tcgataccct aaatgcaaaa
     5941 gacaatatcc tattgcccct agcagttgaa cggatgacta aagaggaaat ggaaaagaga
     6001 gtaagacatg tagctcaatt gttgaatata gaagaactgc tcaaacggta tcccgatgaa
     6061 ctttctgtcg gacagagaca acgaattgca gctgctagag cacttgttgt tcagccacaa
     6121 gtgatttttg cagatgaacc aactggctct ttggactcga agtcggctac agaattgttg
     6181 aactacttga agaccatgaa tcaaaaagaa aaagcaacga tactgttagt cacccatgat
     6241 ccatatacag caagctactg cgatagaatt ttatttatta aagatggtgt gattttttct
     6301 gaagttgtca gaagagggac aagaagagag ttctttgaga aagtaataga catgcaggcg
     6361 acgattggcg gaggaggaaa aatgaatgct gtataaacta gccgttaaaa gtttttttcg
     6421 gcagtcacga ggctatcttg tttatttttt cagtttgact ttatccgtaa tgatctatta
     6481 ttccttcagc gcaatgacct acgatcaatc tttagtcaga agagcaagtc aagatacttc
     6541 gatagatggt ggattgagtt ttggtggatt tatcattact gttgttttgc tttttttcgt
     6601 attcagtgcc aaccgatttt tcttgaatag aaggcaaaag gaaataggca tctatcagct
     6661 ttttggtatg aataaactgc agatatcagg tatatatgtg ttagaaatca tgataattgg
     6721 attgttcgcc tgtatttctg ggatattact cggtattatt ttttccaaac tcttttcaat
     6781 gattttggtt cgaatgatgg atatggatct tactagtcca ttttttatct ccattccatc
     6841 agtagtagat actttatttg cttttttcat catcctttta gcagtatctg tatattcaat
     6901 atggaaaata tggcgctatc caatgattcg aagttttgga gaaaaagaac aagcagatag
     6961 ttcaacaatg cgcttcagga caaggcaccg attactcgca gtcatcgggt tactactttt
     7021 aacgagcgga tattttggag ctagtcattt tagggaaatc acttcatgga tgatcagtta
     7081 tcattatctt gatacattag ttttcttttt tcctttttta atttttttcg tttgtgtggt
     7141 cggtacttat ttgtttttct gttatacatt tcggttattt ttgatcatat tgagtaaatg
     7201 gaagatttat tactatggta tcaatttttt gataatgggt aacacgcaag ttcatttgct
     7261 caaaagttgg cgtacaaatt ctctgattac agtcattctc ggaatttctt taatgacaat
     7321 tggcggcacg gctagtatct ctactatttt atcctaccag gagaaagttt cctccccgat
     7381 ggactatcag tttgatgttg agacagagaa aaaaatcaag ccgattctag cagaggaagg
     7441 gcagaagata actaaagaaa tcaccttgca ttataaagtc attgggagta tctatagtca
     7501 aaatctcgaa gaacctatgt ttcaaactgc taatctgatc acggaaacac aatatcaagc
     7561 cttcagaaaa gttaatccag atttgccgaa catttctttg aaatcacctg gtcatactgt
     7621 tttgcttgat caaatgcaaa ctctttttcg gaatatttca ttatatggat ctagtattgg
     7681 actgccagat aatcaagaat tgaagattca acaaatgctt ccaagtgtat taggtgacga
     7741 aacaatggta tatctaagtc ctgtactgat tgtagatgat accgtttttg aaaaagcaaa
     7801 aggtgtggat tatcaagtag tcaatgtaga tgtatcaggt ggaaataatg aaaaaataga
     7861 tgaacgactg agcaatgacc tgtcgataaa gtggctcaat gctgtttatt attcttatga
     7921 tatcgtaaat ggaagtctac aaggagaaat tagcacaaag aaacttccgg atgaccaaat
     7981 gaaggaagca caatttagcc aagaaagctc tcgtttgaat ttctcttcca gctattcgaa
     8041 attgcgtgca gttaatcggc gaatcggtat caatattttt gtcacactat ttgtcgggat
     8101 gatcgtgatt attgctactg gtagtatttt gacagtccgg caattagcag aagcagagga
     8161 agaaaaagaa aattatcacc tattgactaa gctggggatt ccacaaagaa ggatcagacg
     8221 gatgatcttc gagcaaaacg cacttacctt ctttccaccc atgatattag ggatcactca
     8281 cgcagtattt gcaatcaatg tcttcactca gtatataaaa actgccgatt actggttggc
     8341 ttatcttgtg agtggcttat tgatccttat ctatcttttg ctttatgtca ttacgagtca
     8401 gttatattgt cggattatcg aggaatgata tacggaataa gacaagataa taaatagaac
     8461 taaattttga ataccattta tttggaataa gaggttgtga cacgattttt gtcacaacct
     8521 ctttattttt t
//
LOCUS       DAZUMM010000097         8405 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000097 DAZUMM010000000
VERSION     DAZUMM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.100"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(97..1065)
                     /locus_tag="ACG8E6_002342"
     CDS             complement(97..1065)
                     /locus_tag="ACG8E6_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFS5062720.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1297..2130
                     /locus_tag="ACG8E6_002343"
     CDS             1297..2130
                     /locus_tag="ACG8E6_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFS5062721.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2164..3003
                     /locus_tag="ACG8E6_002344"
     CDS             2164..3003
                     /locus_tag="ACG8E6_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5062722.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            3029..3589
                     /locus_tag="ACG8E6_002345"
     CDS             3029..3589
                     /locus_tag="ACG8E6_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5062723.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            3788..5509
                     /locus_tag="ACG8E6_002346"
     CDS             3788..5509
                     /locus_tag="ACG8E6_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFS5062724.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5674..6816
                     /locus_tag="ACG8E6_002347"
     CDS             5674..6816
                     /locus_tag="ACG8E6_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS5062725.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            6832..8043
                     /gene="thiI"
                     /locus_tag="ACG8E6_002348"
     CDS             6832..8043
                     /gene="thiI"
                     /locus_tag="ACG8E6_002348"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFS5062726.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         2881 a         1454 c         1743 g         2327 t
ORIGIN      
        1 tgtttctcaa atttttgtaa attttcgact tagttttcga agaagctatt tttgtcacag
       61 cctctctttt taagtttagc tattagataa tgatgtttag ttttggataa acgaaatcaa
      121 ttcttcaccc gacaaaccat agtttacagc aatcaatagt tttaaacgtg ctttttgact
      181 attaatactg ttacagaaaa taatccccat tttttctagt tcttttcccc cgccttcata
      241 atcataaaca ggctctgcga ttccattgaa acaacgagaa acaagtacta cagggatttt
      301 ttggacaaga agcttttcca aagcagctaa ggcttgtgga gggagattcc ccgcacctaa
      361 agcttcgatt accaaaccgt ctactttcgt atgggcgatg gcttccaata agtccccttg
      421 catcccagca tatgctttaa cgatcgggat agtcccatct acagcggaaa tgtccagtcg
      481 ctttgtttct aaaagttcct gcaagaataa aattcgattc ttagttacta agccgatggg
      541 acccaatgta ggtgttcgga aagtagcaac atttgtggta tgggtctttg tcacgtagcg
      601 tgcagaatgg atctcatcgt tcatgaccac caaaaccccc ttatctagcg cttcttcaca
      661 acttgcgaca cgaatcgcac tttcaaaatt ataaaggccg tcacttccaa gttcattact
      721 tgaacgcatt gccccagtca aaacaattgg taatttgttg ccaatcgttg tatcaagaaa
      781 aaaagcagtt tcttctaatg tatctgtacc gtgtgtgatc accactccgg atatacctga
      841 tgcataagcg gacaagatac gttcttttaa taccaacatg tgagaaggca tgatgtgtgg
      901 tgagggaaga ttgaagatat cttcaactaa taattctaca tgcttgggga ttgtgatttc
      961 tgcatctaat aatgggtttg ccacatcagg actaacaccg cccgtgagca tgttttcttt
     1021 catggcgatc gttccgccgg tgtgtagtac aagtattttt ttcattttac agacttcctt
     1081 acgaataatt ttgcatagcc gttaaatctt attgtatact atataaaaga gttttgctat
     1141 cagtgaagaa taacgaatag cctaacttat cataaaaaag ctgaccttat taaaatggaa
     1201 aagactcaac cttgacctcc taatacaatg gagtatctgg ttgagtttct tttacaagaa
     1261 agcagccaag gaaatcaatc acgaaggtgg ccggaaatga tcagaatcgg attaacatcg
     1321 tttaaagaac atgacaaatt aactggtaaa aagaataaca ctctttatga atatgcaggg
     1381 tttctaccct tagtggaatt agatactgcc tattatggaa ttccccgaaa gtcctcagtt
     1441 gagaattgga taagtgaagt acctgaatca tttcgctttg tagtcaaagt ctatcaagga
     1501 atcagcggtc aaggagactg gaagccttat tatcagaccg aagaagagat gattgcgcat
     1561 tttttagatg ttatgtcgcc aatggtagat gctggaaaga tgttttgctt tttagtccaa
     1621 ttcccctcat cattcaaatg tacgaaagaa aatgtccagt acttgcgcaa acttagaaag
     1681 tggtttgagg gttatcctgt ttctatagaa ttgcgggatt attcttggta cagcgatgaa
     1741 tttcttgaac gtacaagaaa ctttatgtct agagccgctt tttctttagc catcatcgat
     1801 gagccacagt tgctgaatac aacgattcct tttgatccat ttgtcaccaa tccgaacttt
     1861 gctctttttc gattccatgg taggaatgcg caaggatggc aagcaaatga taaagagtgg
     1921 cgtaaaaaaa gaacgttata tcgttattca gaaaaagaac tgcaagaact agcacatgaa
     1981 gtcaaagtag ttgattctaa gacaaaagag gtaggtgtga tttttaataa taattctggt
     2041 ggtgatgcgg ctgaaaatgc attagcactg caaaaaatat taaatatcaa gccagatgga
     2101 ctcaatccaa cccaaatgga tctgttttaa gggcttattt tattcaagaa aggggcgaag
     2161 tctatgatca aagctatttt ttttgatatt gacggaacat tggtcaccaa tcgttcaaaa
     2221 gcgttggaat caacaaaaca agcaattgaa tatgctcgga aaaacggcat tttatgcggt
     2281 gtagcaacgg ggcgcagccc agtaaagatc aaagaaatca ttgatgaatt ggaattagat
     2341 atgtatgtcg tgtataatgg tcaactggtt tttactgcag atcgcacgat tatcgaccac
     2401 ccttttgaac aaaaagtatt ggaacatatt gttgaatttg cagatgagaa ccatcgacaa
     2461 atcgtttttg gtgcgagaaa tcggttagat ggatcaacga cgatgctatt aggacaatcg
     2521 atttttatca agcgactagt cagcttttta ccgcgtaaat tcccggtacg tctgatgaaa
     2581 aaaatcctcc aggtttttag ccctcaccga caaaaggatc gttatgaaac gttagagata
     2641 ttaaaagaac caatttatca atgtatattg ctcagtccgg agtcagaaca gaaaaagtta
     2701 gaacatcgct ttcccgagtg tacgtttcag cgttcaaata cgtatactgt agatatcatc
     2761 ccaaaaggtg gttcaaaatt actaggcatc caagctttcg caaatgctgt cgaaattgaa
     2821 atggaagaaa ttatggcatt cggcgatcat tacaatgata tcgagatgct gaaaggtgta
     2881 ggaattggag tggccatggg gaatgctcag atcgaagtaa aacatgctgc agactttgtg
     2941 actcaatcaa atgaagaaga tgggatatac gaaggactga aacacttcgg tctgatttat
     3001 tagtcaatga aagaaggaaa aaatcagtat ggaagcagag acaccttttc aaaaagtaga
     3061 aacattccac aaaacttttg atccaagaag accagagata cccacggctt ttacagaaaa
     3121 tgaagctcat acacgtgctg cgttcaaaat agaagaactt gttgaatttg ttgcagctac
     3181 ttcacctaat aaagaacaac tgcatgaagc tgttatgctg ctgcacgaag cattagataa
     3241 agcagaagaa aaaatacttg gtaaaacaca gtggggaagt gatccgctgg ttgaacaagt
     3301 agatgcactg acagatatcc tttactttac ttatggttct ttttcattac taggaatagt
     3361 gcctgatcga atattcgaaa tcgtccatca ggcaaatatg gggaaattat ttccagatgg
     3421 aaaacctcat tatgaccctg taacaaataa agtgttgaaa cctgaaaact ggcagatgga
     3481 atatgctcca gaacctaaaa tcgcggcaga aatccaacgt cagattcaag aagcgattgc
     3541 taagaagaaa tccgctgata taggcatgtg cacaaatgaa aaagagtgat cagagtttca
     3601 ctatttttgt tatctttata cgatggaaaa acaaaagatt ttcgtttaag ttagtgaatg
     3661 catgaaaata gtatgctata atctagaata gatgtctttt gcacgagaga cgtattattt
     3721 ggatttatgc caagcagaac gccatctgtt gcataaaaga tgcatactga aaacggagga
     3781 accataaatg ggaaataatg ttatcatcgg aatcgtcatt gcaatcatta tagtcgccat
     3841 tgtcgtcttt ttggttagtt acttgatgaa gaaaaaaaac caagatcgat tggatgcctt
     3901 agaaaaacgc aaggaagaat tatttgactt gccagtgatt gaagaagtcg atgatgttaa
     3961 aaaaatgcat ctggttgggc aaagccaaaa tacctttcgt gaatggagtc aaaaatggaa
     4021 tgacatatct acttcctcgt tcgctgaact agaaagtcaa attttcgaag tggaaaacct
     4081 caatgaaagt tttcggttct taaaaggaaa aaaagcagtt gttgaagcag aagctacaat
     4141 ggaccaaatg gagaaacaag tgacggaaat ccgtgaaggt ctaaaggaat tgcgggaaac
     4201 ggaagaacgc aactcactag ctgttcagca ggcacttgac gtctatgaag aactgaaaga
     4261 aaaagtcaga gaagacgagc atgaatacgg accagctctt tttgaattgc aaaaacagat
     4321 caaagtgatc gaaaaagaat ttacgcagtt tgtgacttta aacacttctg gagaccctgt
     4381 tgaagcccgc gaggttttgg ataaagcaga aaaacatacg tatgaagtgg aagctctgat
     4441 gaaaaaaatt ccaccgctgt atgaggagct ccataccact ttccctgaac aacttgaaga
     4501 aatcagtgat acttacgaga aattgatgga agaacaatat gtattcccag aagaagatct
     4561 tgctgaagat atcgcaaaag taagtcgaag aattgaaaat tcattagcca atcttgaaaa
     4621 aacagaagtt gaaacggtcg aatttgaaaa ccgtgaaaca gctgatttga ttgattcgtt
     4681 atatgatatc ttagaacggg aaatggaagc tcagagatat gtaaaaacta accaatcaac
     4741 gattgcagag tatatcaaac atacaacaaa gaataaccgt caattattga ttgagctgga
     4801 tcacacatct caaagctata cattgaacca caatgaaatt ggccgtgtac gaggtttcca
     4861 aaccgaagtg gaagaaatgg aacgtcaaaa tgaacagatg attccacaga tccgtcaaca
     4921 cgaaattcct tattcagaag tgcgcacgtt ctataaaaca gtcttcaaag tcttagaaga
     4981 tatcgaaaag caacaagtag agatcgacga ctcattacat gaattgagaa aaggcgaaaa
     5041 agaagcgcaa gaaaagatcg atacattcga attcaaattg cgcagtttga aacgttttgt
     5101 agaaaaacaa cgtttgcctg gcttgccgaa tgattaccta gaattctttt ttgttgctac
     5161 tgaccgtatt gaagaactaa gtgttgtctt gaataaaatt cgagtaaaca tggaagaggt
     5221 caatcgttta gttgctctat gtgaagaaga tctagaatta ttagataaga agacacatga
     5281 tttagtagat gcagcagcat taacggagca aatgctccaa tatgcaaatc gttatcgtca
     5341 tacacacgaa gaagtgagag aagcaattga taaaagctac tatttattca ataaagaata
     5401 tcactatcaa gaagcattag acgaaatcgg tacagcatta gagcgagtag aacctggtgc
     5461 tttcaaacgc atcgaagatt tttattttaa tcatccagac ttagtataaa gcaaaaaagt
     5521 tagtaaacca gcaagagcaa gacggattat tgtcttgttc ttgtcttttt ttgtatattt
     5581 gaaaattgaa acaagtgaaa atttcacctg ctcttgaaag caccttggac aaccgttata
     5641 atagaaaaga atttcaaaaa agaggggttt cttatgatat attttgataa cagtgcaaca
     5701 accccaatct ttccgcaaag cttagatgca tatgtaaaaa ccagtcagcg gattattggg
     5761 aatccatcaa gcttgcatga tttaggcaat caagctaacc ggttgttgca gcaagcaaga
     5821 aagcagatcg ctgatttgat ccatgtagaa ccagaagaaa tctattttac ttcaggcgga
     5881 acagaaggag ataactgggt attaaaagga acgatgatcg aaaaaagaga atatgggaat
     5941 catatgatca tttcaggagt cgaacatccg gccgtcagcg agacagcaga acaattaaaa
     6001 gaattaggaa tcgaagtaag tatcgcccca gtggataaac gcggatttgt acgtgtcgac
     6061 gaattgaaag aattaataag aaaagagaca gtattagtct ctgttatggc tgtgaataat
     6121 gaagtaggat cgattcagcc catccaggaa atcagtgatc ttttggatga ttttcctaaa
     6181 attcatttcc atgtagatgc tgttcaggcg attggaaaag taccattctc gcaatggttg
     6241 acagaaagag ttgattttgc aacattttca gctcataaat tccatggccc acggggaact
     6301 ggtttcattt attggaaaaa gggtcgtcgt ttagcaccat tactgacagg tggagggcaa
     6361 gaaagaaatc agcgaagtgg gacagaaaat gttccaggta ttgtggctat ggttaaagct
     6421 ttacgtttat cattagagat aaaggaacaa aaacctgagc agacacaacg cttaaaagta
     6481 gctctaatca atgcgttgaa gacgtatgag aaagtgacga ttttttcaga aggaactgac
     6541 tatgcaccgc atattttatg ttttgctcta aaaggaattc gcggcgaagt tctcgtccat
     6601 gccttagaag aaaaacaaat atttgtttct actactagtg cctgctcctc acgtaaacat
     6661 atggcaagca gtacattgca cgcaatgcat gtaccaaatg atctggctac atctgctgtt
     6721 cgaatcagct tagatgaaaa caattcgctt gcagaagtcg agcaatttat gattattttc
     6781 aagcagcttt ataagaaatt ttctgcaatc aattaaaaga ggagtgttct tgtgaaatac
     6841 agtgaaatta tggtccgtta tggggaactt tcgacaaaag gaaagaatcg aaaaagcttt
     6901 atcatgcaac tagcacaaaa tgtcagacaa gctttgaaag actttccaga aatcaaaatc
     6961 catgctgatc gggaccggat gcatctttta ttgaatggcg cagacgggca gctagttatt
     7021 ccaaaactag caaaaatatt tgggatccaa aatttctctc caagtattcg tgtagaaaaa
     7081 gatgtcgaag ttttgaaaca agctgttcaa gacatcatga aagaaattta tactgggaaa
     7141 gaaacattta aaattatcgc taaaagaagc gatcaccaat ttgaattgac aagtaatgaa
     7201 ttgaatcaaa cattaggtaa tgctgttttt gatattttcc cgcatatcca agttcaaatg
     7261 cgtcaaccag atattccatt acgtgtagag attcggcgtg atggcgctta tctatcgtat
     7321 gaaacgatca agggagctgg aggattgccc gtaggaacaa gcggaaaagg aatgcttatg
     7381 ctttctgggg ggattgattc tcctgtagca gggtactttg ccatgaagcg gggagtggaa
     7441 attgaagctg ttcattttgc tagtccgccg tatacaagtg aacaagcact gcaaaaagca
     7501 aaagatctaa cagccaaact agcaccttat gtgggaacga tccattttat cgaagtgcca
     7561 tttaccgaga ttcaagaaga aatcaaaaag tcagtgccac aaggatattg gatgacaatt
     7621 actcgtcgaa tgatgttgcg tttgaccgat gaaattcgcc gtattcgtca tggtttagtc
     7681 attttgaatg gtgaatcatt aggtcaagtg gcttcacaaa ctttgcaaag tatggtggct
     7741 atcaatgaag taacgaatac gccaatcatt cggcctgtag cgacaatgga taaacttgaa
     7801 atcattgaag tagcagaaca gatcgataca tttaatcttt ctatccagcc ttttgaagat
     7861 tgctgtacga tttttgctcc tccacagcca aaaacacgcc ctcaactgga aaaggtccat
     7921 caatatgaag agcgtttagc aatagatgaa atgatccaac gagcactagc tggcttgaag
     7981 atggaagaga tacaagcaga atcgtctaat gaaaaaactg aagaattttc agaattgcta
     8041 taagaggtta tgaaaatagt gttttgacca gagtattaga gaaacaaaac gaaaaaatag
     8101 ctccatgtat tttaccgttt ttgaactaat gtcgaagatt agctgattga ataccattta
     8161 tcaagaagtt atggaaaagc cggttctttt ttactaactg aaaaataaaa ttttatttag
     8221 agatcaagct acagttgata aatgatataa aaaagagaat ttcccatgaa atagcgaggt
     8281 tgtgacagaa gtgggcagct tcaagaaata agaaggaatt tacgaaaatt gcttttcaaa
     8341 tttttgtgaa atttcggctt atttttcgaa gaagttactt ctggaacacc gtgtatcaga
     8401 ggttg
//
LOCUS       DAZUMM010000098         8370 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000098 DAZUMM010000000
VERSION     DAZUMM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.101"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..2199
                     /locus_tag="ACG8E6_002349"
     CDS             7..2199
                     /locus_tag="ACG8E6_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFS5062727.1"
                     /translation="MLNKTLQKLQKKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            2216..2692
                     /gene="ybeY"
                     /locus_tag="ACG8E6_002350"
     CDS             2216..2692
                     /gene="ybeY"
                     /locus_tag="ACG8E6_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFS5062728.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            2676..3071
                     /locus_tag="ACG8E6_002351"
     CDS             2676..3071
                     /locus_tag="ACG8E6_002351"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5062729.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFSVIVGLILFVPKIYHLLFN"
     gene            3086..3985
                     /gene="era"
                     /locus_tag="ACG8E6_002352"
     CDS             3086..3985
                     /gene="era"
                     /locus_tag="ACG8E6_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFS5062730.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            4128..4940
                     /gene="recO"
                     /locus_tag="ACG8E6_002353"
     CDS             4128..4940
                     /gene="recO"
                     /locus_tag="ACG8E6_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFS5062731.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTSEKDMSEKDLPKKEE"
     gene            5326..6243
                     /gene="glyQ"
                     /locus_tag="ACG8E6_002354"
     CDS             5326..6243
                     /gene="glyQ"
                     /locus_tag="ACG8E6_002354"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFS5062732.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            6245..8320
                     /gene="glyS"
                     /locus_tag="ACG8E6_002355"
     CDS             6245..8320
                     /gene="glyS"
                     /locus_tag="ACG8E6_002355"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFS5062733.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         2782 a         1537 c         1824 g         2227 t
ORIGIN      
        1 aagcacatgt tgaataaaac actgcagaaa ctacaaaaaa aactcggaaa ggcagcattg
       61 cctttccttc tgttcttgtt ttttatcatt gtctgcggcg ttacatttag tagtgtcaaa
      121 caaaaggcca atgatttcag agaaggccaa gtagcagaag agagtatccg tgcgaataaa
      181 acgattgaga acacggaaga aaccgaacaa agaagaaaac tggcagcaga agcagtgact
      241 ccagaatata cttatcaaaa agatctggca gatgaccaga acaatcggat caagcaatta
      301 ttcgaattga tcgataagac gaatgatacc atcaacaaat cttataatga caaggtagat
      361 aaggcgaagg aagatgaaaa tatcccgaaa cctactacag aagagcggat tgctacactg
      421 aagaaaaatt ttgaaaacgt gaatgcagaa aacgttgcgt tttaccaaaa actgccgaac
      481 aatttttatt cgacgatatt tgaaatgact gaaacggaaa ttcacacggt tagggatgaa
      541 agtttgaagc tcattgacga acagatgagt aaacaagttc gtgaaagcga attggaagct
      601 tttaaacaag aagcagaaga tcagattcaa tatttgaatg tcacaacaga gcagcaacaa
      661 atgattcgct acttagtgga tcaaggcatt gtagttaatg atgtattgaa tgagaaaaag
      721 acagaagaat taaaacaatc tgcacgagaa gctgtacaac cagtgatgat cttccaagga
      781 gaaattattg ttcgcgaagg aaatcaaatt gatgcttcag cgatgaaaaa attggaatta
      841 ctaggattga cgaatcaaac gacttctatt ttcccactca ttgccatggt tttagctgtt
      901 ttgcttcaaa tcggtgtact gatttataat tcacttcaat tccaaaatgc aggagaaaga
      961 acaaaatacg tacttttcta cgtgacggca atgtcagtaa gcgtcgtact aatgaaattt
     1021 ttccaactct tccaaacaga acaagctgct tatattccgc tgttttaccc cgcggctttt
     1081 gctccacttg ttttaagctt tttcttgaat cgaagagcag ggatcatggc tggtattttt
     1141 caagcagtat ccgcactttt cattttttat gattcgattg gcacgaactt tctgacagtt
     1201 attttgatgt cctatgtatt ttctggtctc atggcaacga tcgtcagacg caaacggatc
     1261 tcagaacaag ggttatccgc tgctatgtgg gtcattattt ttcctgtgat gatggatgtc
     1321 attttgatta tctaccaagg aatgagcttt ggggatgcaa cgacttggtt aacgctagta
     1381 tgcggattca caggtgcact gttctcattc ttgctgacga taggtctgca cccctatatt
     1441 gaattgatgg tcaatgatga tagtgtgatc gtattaaatg aactcagcaa tcctaatcat
     1501 cctttattga aacagttgct ggaagaagca ccaggcacgt atcaccatag catgatggta
     1561 gccaatctaa gtgctaatgc tgtagcagaa atcggtggac gttctttact aactcgagta
     1621 gcttgctatt atcatgatat cggcaagatc aaacacgcca gctttttcgt tgagaacttg
     1681 ccttcaggag cggaaaatcc gcacaatttc ttattgccag aagacagtaa acatattatc
     1741 tttggacatg tgacagatgg tgccaaaata ttggaagatt ataatatgcc gcaaatggtg
     1801 atcgatattt gcagacagca tcatggaacc acgctgatgc gttatttcta cgttaaggca
     1861 aaggaacgca acccggaagt cacggaagag caattccgtt atcctggacc aaaacctcaa
     1921 agccgtgaag caggaatcgt caatatagct gacagttgtg aagcggcagt acgagcaatg
     1981 gaccatccaa cgagcgaaaa aatcgaaagt tttgtccata atcttattga agagcgtatt
     2041 tccgatggac aattggacga cagtggattg acgttaaaag aaatccgtaa agtagaaaaa
     2101 tcattgatca gcggtttaag cagtaccttc cattctcgga tcaaatatcc taaaatgaaa
     2161 tcggaagttg agaagataaa agaagaacaa gaagaataga aagaagcgat gtataatgga
     2221 tattactttc atggatgaaa caaaccaagt ctcagaagag aaaatgaaag aaatcgacga
     2281 cttgctgcaa tttgcagcat catatttaga gttgccagaa gaaacagaaa tgtctgttac
     2341 tttcatggac aatgcagcga tccaagtaat caatcgtgat taccgtggaa aagatgcacc
     2401 gacagacgtc atcagttttg ccttggaaga agagggcgaa gacgaaatcc cagtgatttt
     2461 tgaagatgga gagaatccat tgcctcgtgt actgggagac atcatgatct ctactgaacg
     2521 tgctgcagag caagcagaag cttacggcca ttcgtatgat cgggaacttg gctttttagc
     2581 ggttcatgga ttcttgcata tcaacgggta tgaccatatg acaccagagg atgagaaaga
     2641 aatgtttggt ttacagaaag agatcttaga tgcctatgga cttaaaagat aaaaacgtag
     2701 agaaaaacaa acatttcctg atgtccgtgg aatttgccgt ccaaggagtc aaaaccgttt
     2761 ttgaggaaga acgcaacatg aggaaacatg ttgcgttcgg aatcctcgct ttgtttatgg
     2821 gcgtgatttt ccagcttgat cggatagaat ggttatggct gcttttagct gtttttctcg
     2881 tatggatagt agagatcatc aatactgttt ttgaaaatgt cgtggacatg tttacggatt
     2941 ttcattttca tccgattggg aaaaaaatca aagatatggc agctggagca gttttagtga
     3001 ccgcttgttt ttccgtcatc gttgggttga ttttatttgt ccctaaaata tatcatttgt
     3061 tgtttaatta aaaaggagaa cgtatatgcc agaacataaa tcagggtttg tcgcaatcgt
     3121 cggccgcccg aatgtaggaa aatccacatt attgaatcga atcgtgggtc aaaaaattgc
     3181 catcatgagt gataaggcgc agacaactag aaataaaatc caaggagtat atactacacc
     3241 agaagctcag ttgattttta ttgacacacc aggaatccat aaaccaaagc atcgtttagg
     3301 agattttatg gtagaagcag cttacagtgc gttgaaagaa gtggacgcgg tcttatttat
     3361 gatcagcgct gatcaaaaac gcggtcgggg agacgatttt attattgaac gtctaaaaaa
     3421 cgtgcattct cctgtttatc tgattatcaa taaaatcgat aaaatccatc cagacgagtt
     3481 gttgggaatc atcgaagatt attcaacgca gatgccgttt gcacaagtga ttccaatttc
     3541 tgctacagaa ggaaacaacg tcgaacgttt gatggatgta ttagtcagtg aaatgccaga
     3601 gggcccacaa tattttccag atgatcaagt gactgatcat cctgaatact tcatcgtttc
     3661 tgaattgatc cgtgagaaag tcttgttttt aacacgagac gaagtgcctc attctgtcgc
     3721 agtcgttgtt gattcgatga agcgaaacga gaatgataaa atccatatcc aggctacgat
     3781 tattgtcgaa agagatagcc aaaaagggat cattatcggc aaaggcggaa aaatgctgaa
     3841 gcaagtagga acgaaagcga gaaaagatat cgaaaatctt ttaggagaca aagtctttct
     3901 agaactttgg gtcaaagttc aaaaagactg gcgagacaaa aaagtctatc tgcaagactt
     3961 cggctaccga aaagacgaat attgatattc tgagcttctt gtaagttagg tatagaaata
     4021 aactggataa aaatccgcgt gttgagaaaa gctgcggaag atcgcaacat aaatgatctg
     4081 gatcggcaga atctgctttt ccagatttat tttttaggag ggagaatatg agccagcttg
     4141 cagaatcaaa aggcttgatt ttatctagcc gtaattacaa agaaaaagat aaattagtca
     4201 aaatctttac ggaatcttct ggcaaaatga tgttttatat caaagggatc catcggcaaa
     4261 atcagccctt agctccggct ttgctccctt ttactgaagc agtttatatc gggaattttc
     4321 gaagtgaagg tttgtcattt ttaagcagtg tcaaagatgt ccacgcgttt cgacacatcc
     4381 aagaagacat ctttttgagc ggctatggca catatttatt gaatctagta gatgcggcaa
     4441 tcgaagatca tcagtacgac ccgcatttgt tccagtttac gcaacaagca ctgcaacgga
     4501 tggaccaagg agatgatgca gaaatcatca cgaatatttt tgaagtccaa atactgcaac
     4561 gcttcggaat tgcaccaatc tggacacact gcgtcgtatg tggggaaaca aaaggaaaat
     4621 ttgactattc ttccaaatat ggtggtgtga tatgcgaaag acactggtcg atggatgatc
     4681 accgttatca tgcagatcct cgagctgttt atttcgttcg gatgttttca gctatttctt
     4741 atgataaaat cagtggaatc catctgaaag aagaaaccaa acaagcaatc cgtgaactca
     4801 tcgaccagct gtatgatgaa tatgtcggta tccatttgaa aagcaaaaag ttcattgatc
     4861 aaatgaagtc ttgggcacac gtattaaaga cgtcagagaa agacatgtct gaaaaagatc
     4921 tacctaaaaa agaagaatag taaatgaaac atacaacaaa aaatcaaaac catttgacaa
     4981 acacagggag ttccgtctat tattaaccgt agatcaaata gatcgttcac tgagaggatc
     5041 tattatagaa acaagaaaac tgcaaaatga atacgtacat tgaccaagat cagtaaaaaa
     5101 cacgtggttt agcgagttcg ggagagtggg agccggaccc aaacgatttt tgaagaggca
     5161 cttgcgagta cgataaatga agctgccgaa tctttattcg gaagtagggt ggaaccgcga
     5221 attattcgtc cctatgtctg taatcagaca tagggatttt ttgtcgtttt ctttcctgta
     5281 ttcttatttg acagcaacgc tttaaaattg aaaggggcaa aacagatgag taaaaaattg
     5341 accgtccaag atatgatttt aacattacaa aaattttggt catcaaacgg ctgcatgctg
     5401 atgcaagcgt atgacacaga aaaaggagca ggtacgatga gcccatatac ctttttacgg
     5461 gcaatcggac cagaaccatg gaatgctgct tacgtagagc catctcgccg accagctgat
     5521 ggacgttatg gcgaaaaccc aaaccgtttg tatcagcatc atcaattcca agtcgtgatg
     5581 aaaccttcac ctgagaatat ccaagagctt tatttagaaa gcttgcgcct tttaggaatc
     5641 gatccactag aacacgacat ccgttttgtt gaagacaact gggagaaccc atccatgggc
     5701 tgtgccggtg tgggttggga agtatggctt gacggaatgg aaatcacaca attcacgtac
     5761 ttccaacaag ttggtggact gccttgtaaa ccagtaactt ctgagatcac ttatggatta
     5821 gaacgtcttg cttcttatat ccaagaagtt gaaagtgtct atgatttaga atgggctgat
     5881 ggcgtgaaat atggcgagat cttcaaacaa ccagaatatg aacattctaa atattctttt
     5941 gaagtaagcg accaagactt gctactaagc aactttgatc gttttgaaaa agaagctaaa
     6001 cgctgtatcg atgaaaatct tgttcatccg gcgtatgatt atatcttgaa atgtagccat
     6061 acattcaact tattagacgc ccgcggtgca gtatccgtaa cagagcgtgc cggttatctt
     6121 tctcgcatcc gcaacatggc tcgtgctgta gccaaaatct ttgtggcaga acgcgaaaaa
     6181 ttaggattcc ctttgttgaa taaaaacaca gcaaatccag aaaacgtgaa ggaggctgaa
     6241 taagatggca aaagacttat tacttgaaat cgggctggaa gaaatgcctg ctcatgttgt
     6301 cacaccaagc atgaaacaat tagaagaaaa aacagcaaaa tttttagatg aaaaccaatt
     6361 gacttatgat acgatcgaaa cattttccac accgcgccgt ttagcaatca aagtaacaaa
     6421 cattccagaa cgacaagcgg acagtgaaga agaagtcaaa ggaccagcca aaaaaattgc
     6481 tttggacgca gatggaaact ggacgaaagc agcggaagga tttgtccgcg gacaaggtct
     6541 gacgacagac gctatcactt ttagagaatt aaaaggtgtg gaatatgtct atgtaacgaa
     6601 atacactcat ggtaaggaat ccaaagaagt attgacaggt ttagcagatg tcattaaaag
     6661 cctgactttc cctgtaacga tgcattgggc agaatacgat tttgaatata tccgccctat
     6721 tcactggatc gtggcattgt tagatgacga agtaatccca ttcgaaatct tggatgtcaa
     6781 aacaggcaga gcctctcgag gtcatcgttt cttaggcgaa gatgtgacat ttgcgcatgc
     6841 aaaagaatat gaagaaaaat tgaatgaaca attcgtcatt gcgtctccgc aaaaaagaca
     6901 agaaatgatc gttacacaag ccaatgaatt agccgaaaaa aatcattggg ttattgatct
     6961 agacgaagat ttactagaag aagtcaacaa tttagtcgaa tatccaactg tcttttctgg
     7021 cgacttcgaa gaaaaatatc tttctgttcc tgaagaagta ctagtcactt ctatgaaaga
     7081 acatcaacgt tatttcgagg tacggaatga tcaaggtatg ctgcttcctc attttatttc
     7141 tgctcgtaat ggtgatcggg taaaattgga aaatgtggtt cgaggcaatc aaaaagtatt
     7201 gacggctcgt ttagaagatg cggaattctt ttataatgaa gacaagaaag cttcaatcga
     7261 ccaatacgtt gaaaaactaa aaactgttac tttccatgaa aaaatcggta gtatctatga
     7321 gaaaatgcaa cgggtacaag tcattgcccg tatcattggc gaacaagtag gactatctga
     7381 ggaagaattg atggatctgc aacgagctgc tgaaatctat aaatttgatc tagtaaccaa
     7441 catggtaggg gaatttcctg aactgcaagg gatcatgggc gaaaaatatg ctctgctgaa
     7501 aggcgagaaa cctgctgtag cgacagcgat tcgtgagcat tacatgccta tatccagtga
     7561 aggagagcta cctcagtcta cagtaggttc tgttttagcg attgcagata aattagatag
     7621 catcttttct ttctttgctg ttgggatgat cccaagcggt tcaaacgatc cttatgcctt
     7681 acgtcgccaa gcatacggtg tcgttcggat catcgaatcg aaaaaatggg cctttccttt
     7741 gtctgttctg caagaaacga ttagcgatgt tatcagtaaa gatacggatc gtttcggcat
     7801 tggtttatca gaagggcaac aacaagtgat cgactttatc aaaggccgtc tgcgccaatt
     7861 attgacaacg aagaatattc gtcatgatgt catcgatgct gttttgaatg cggaacaaaa
     7921 agatctgaca aaagtatttg cagcagcaca attgttcaaa caacatcttg cagatgaaga
     7981 cttcaaacct tcaatggaag ctttgacacg tgtcgtgaac ttagcgaaaa aagcgcagct
     8041 tgaaaaacaa tcagaagtag atcctgagct gttcgaaaac gacgcagaga aagaattaca
     8101 caaagcagta gaagcaatga aagcaagttt tgctgaccaa acgatccacg aaaactacga
     8161 ccagcttgtc gctcttcgtc cactgatcga aaattatttt gatcaaacga tggtcatggc
     8221 tgatgaacca gcagtacgta agaaccgact aacacaactt agtcaaatag ctgccatggc
     8281 tttgtcatta gcaagcttgg atcaaatcat cacaaaatag tcttttgttt gaaaaacaga
     8341 gagcatgcac gaaacgtgct gtgtctctgt
//
LOCUS       DAZUMM010000099         8229 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000099 DAZUMM010000000
VERSION     DAZUMM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.102"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="ACG8E6_002356"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="ACG8E6_002356"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFS5062734.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="ACG8E6_002357"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="ACG8E6_002357"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFS5062735.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="ACG8E6_002358"
     CDS             2591..3376
                     /locus_tag="ACG8E6_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5062736.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="ACG8E6_002359"
     CDS             3389..4240
                     /locus_tag="ACG8E6_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS5062737.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="ACG8E6_002360"
     CDS             complement(4364..4816)
                     /locus_tag="ACG8E6_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5062738.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="ACG8E6_002361"
     CDS             complement(5281..5649)
                     /locus_tag="ACG8E6_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFS5062739.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="ACG8E6_002362"
     CDS             5799..6281
                     /locus_tag="ACG8E6_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFS5062740.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="ACG8E6_002363"
     CDS             6318..7328
                     /locus_tag="ACG8E6_002363"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFS5062741.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="ACG8E6_002364"
     CDS             complement(7443..8156)
                     /locus_tag="ACG8E6_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062742.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1338 c         1667 g         2440 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttcc atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtct
//
LOCUS       DAZUMM010000100         8152 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000100 DAZUMM010000000
VERSION     DAZUMM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.103"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            386..574
                     /locus_tag="ACG8E6_002365"
     CDS             386..574
                     /locus_tag="ACG8E6_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFS5062743.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            652..1665
                     /locus_tag="ACG8E6_002366"
     CDS             652..1665
                     /locus_tag="ACG8E6_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5062744.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1732..2766)
                     /locus_tag="ACG8E6_002367"
     CDS             complement(1732..2766)
                     /locus_tag="ACG8E6_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5062745.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2881..5106
                     /locus_tag="ACG8E6_002368"
     CDS             2881..5106
                     /locus_tag="ACG8E6_002368"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS5062746.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5124..7007
                     /locus_tag="ACG8E6_002369"
     CDS             5124..7007
                     /locus_tag="ACG8E6_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFS5062747.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6988..7962
                     /locus_tag="ACG8E6_002370"
     CDS             6988..7962
                     /locus_tag="ACG8E6_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFS5062748.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2644 a         1480 c         1712 g         2316 t
ORIGIN      
        1 tttagttagt ctacattatt ttggcgattt gccaataagg actgttgagt tgtgcaaaat
       61 caaatctggg tcagaatgaa cgtcattctg tctcagattt ttcgttattt caatttgatt
      121 aactgattga tcaaaaagat ttttattttc atattctcaa atgattcaac actaaatata
      181 catgtttgac gcggaaatta ttattcgatt ttggcggcat caggggggat gacgacgtag
      241 cagacccatt attttactca agaaaatgtc cgtgcctatt atttagaata cgatagtgaa
      301 cgtgctggga gttttgttct tttagagaaa gtctctgaag ataaattagt tgtcttagat
      361 tttgactgta acgaaaaatg gcgaaatgga agagcaccag caagtgattg accggatcta
      421 cgaagcgaca cgatatctgc ctttggatcg tttgtgctta agtcctcaat gtggatttgc
      481 ttcaacagaa gaagaaaatg ttttgtttga agaacaatgg aaaaaatttc ttttgtcaaa
      541 agcattgcag aagaagtttg gggaaaatca ttaaatcatc taggagagct tgataggaaa
      601 taacactatg gtataatcag aatgcttaat actaactact gagggagcct tatgaaaaag
      661 aaaattctta aatttatcgg atggttcttc ggaatcattc ttgggatcgc gtttgcaatc
      721 tttcttgcct tccagctttc ccccagacct ggagcattct tgatcaacca tatgttttca
      781 aatactgttc aaatcactga taaaaaaacg ttcgatcaag cagaaaaaaa cgtggcgaaa
      841 aaaacagatt tgacgtatga gtccaagtat aaagagaata cttatgacat ctactatcca
      901 aaaaacagca aggggcctgt tcctgttcta ttttgggtac atggtggagg attcgtcggc
      961 ggagataaat cgggtgtcaa agaatttgcg acaaaattag tttcagacgc caacatcgca
     1021 gtggttgcga tgaattatga attagcgcct gattcagaat accctaatca agttcttcaa
     1081 gtgaatgaac tgatcaaaga tttattagct gaagaaaaag acgacaaatt gctagacatg
     1141 gaacaattat tttttggtgg agatagtgca ggttcacaaa tcgcattgca atatgcagct
     1201 atacaaacga atgatgcgta tgctaaacaa atgaaaatca aacagctact tccaaaagat
     1261 agtctgaaag gaaccatttc ttattgcgga cctgttgatt tgaagctaac agcacagcag
     1321 cattcggatg atcgatttat gaagtttttc gtcaaaaccg ttgcgtggtc gttgattgga
     1381 acaaaagatt ggaaaacgag tgagcaattg aaagaagcta gtgtggctga ccatgtttct
     1441 aaggaatttc cgccaacgta tataactgac ggaaatgcat actcattcca ggagcaaggt
     1501 caagcattag aaaatcggtt gacagaatta aaggtacccg tgcaatcctt gttttataca
     1561 gatacaaaaa aagagattac ccatgaatac caatttgatt acacgctaaa agaatcgcaa
     1621 aattgttacc agcagacatt ggattttgtg aataagtata agtaagaggt tgtgaaaaag
     1681 ccgtttcgtt ttgagtatga agaaacgaag cggcttttca caacctctct ttcaatacct
     1741 ttttgttctt gggataaaac tttccctaac aatcaatcgt gcaggataag tcaaaatgat
     1801 tggttcgtcc cgtataccgt caattcgctc caatgccatt cgaacagctg cttgcccaat
     1861 tgatttcgca ttgatttgaa cactggtcaa gccaggggag aggtttgcga ttgtctcgat
     1921 attatcaaag cttgctacaa caatctcttc tccaatttgg atattttttt gtttcaatgc
     1981 ccgatagaca ccaattgcca ttggatcgct cccgacaaga atggcagtag gaagagggga
     2041 ttcattttct aatagattag tcgcaagtac ctcaccttgt ttttctgtcc agccacctag
     2101 atgataagac atatattttt ctaagtgatg atcgatcata aaatgctgat aggcacgcat
     2161 cctcttttca tttatagaaa tctgcttccc accgaattca tccatatcga ctttatatcc
     2221 cccgatatag gctattcttt tatgaccatt ttctaagaac aattccagta cggttttagt
     2281 catccgttca agatctgcat acaccatatc gacctcttgc tggatgtcag ggttatccac
     2341 gacgactatt tgcatgtttt gttttaacaa ggcggtaatc gattctttcg taacagtccc
     2401 aacaacgatc accgcaccta actgatctag atcttcccat tgtttaggat tatcagatag
     2461 attatagatt cgattcatcc ctaaatgcag acgtcgagct tcttcttcca tccctactcg
     2521 caaatgtttg taatagatat cctgttcttc gctcgctttg ccgatcgtag agatcacacc
     2581 aatcgaaggc attctctttt tgatatattt acgagtactg atgtcataat ccaacgcagc
     2641 agctgcctct actatccgtt gccgagtagt ctgagccaca tggatcgtct gatcgttatt
     2701 caacacacgg gaaactgtag caggagagac atttgccttt cgtgcgacat ctctaattcc
     2761 aaccattttt acacactcct caaaaaaata aaaaattttt attgacaaaa ttgtttagta
     2821 aacagtatac aggaattata aaaaaaagta aacggtttcc gttttgttgg gagtgaaatg
     2881 atggatatta cgcatagaaa aacagataaa aatttcatct tatttgatga aaagagccga
     2941 gtcttccatt tacgaaatac atttctttct tatctgataa agatagaaga aagcaatgta
     3001 ttggcgcata tttattttgg caaaccagtc aagcagtaca aagacaataa aaattatccc
     3061 cgaagagatc gtggattttc aggaaatgtt cccttaaatc ctgaccggtc tctgtcgaaa
     3121 gatacactac cgcaagaata ctcttctcat ggcagcatgg actttcgaac gcccgcatcg
     3181 atcattcagc gcaagaatgg atcagactta ctggatctga gatatgacag ccattatatt
     3241 acagatggga agccagagat cgaaggcttg cctcaaactt atgtgatgga caagtcagag
     3301 gcacaaacgt tggtgatttc acttaaggat agagaaacag cgatttattt cgatttgttc
     3361 tataccatat ttactgatag agcagtgatc acacgatctg taaagataag aaatgaaacc
     3421 ggagaaacaa tcaaactaga aaaagcggct agtttccagc tagactttgc ccatactagg
     3481 cgatttgatg aagtcattgc tttgccagga gcacatgtca atgaaagaca gatttcacgg
     3541 caatctgttt tatctggaac aaaagtattt gaaagccgtc gaggaacgag cagtcaccat
     3601 atgaataact ttattgcttt agttcatcat catacgacag aaaatactgg agaagcgatc
     3661 ggacttcaat ttgtttattc cggcaaccat tctttcgaac ttgaaaaaga tcagatcaat
     3721 caattgagag tcgtcggtgg gatcaacagc catcgttttt cgtgggaatt gaatgcaggc
     3781 cagtcttttc aaacacctga aatgatcctt tcttactcaa gtcagggatt gaacaaaatg
     3841 agtcagatcc accatgagtt gctacgtgaa agaatcgcaa gaggtcgtca tcaatttgct
     3901 gaacggccga ttctggtcaa taactgggaa gccacttact ttgacttcaa tagtgaaaaa
     3961 atcaaggcga tcattgatga agcaaaggag ctaggcatcg aaatgttcgt attagatgat
     4021 ggctggtttg gaaaaagaga tgcggacaat tcttcattag gagactggtt cgagtatgaa
     4081 ggcaagttga caaatggatt acgtgaaata gcagattatg ctcatagcaa aggattgaaa
     4141 tttggtttat ggtttgaacc tgaaatgatc tcggttgatt ctgaactgta tcgaacacat
     4201 cctgattttc ttatgcagga gccaggtaga atgccttctg cttccagatc acaacatgta
     4261 ctggatttta caaggttaga tgttcggcag acgattgaga aacaaatgag aaagatttta
     4321 gatacgattc ctcttgacta catcaaatgg gatatgaacc gttcgctttc agatgtctat
     4381 tcgattaccc ttgacccaca gagacaagga gaagtagcgc atcgttatat gctcgggctt
     4441 tatgaactac ttgaacattt gacaactgat tatccggaaa tcttatggga aggctgttct
     4501 ggtggaggtg gacggtttga tgccgggttt atttattata tgccgcagtc atggacaagt
     4561 gataacacgg atgcagtgga gcgtatgaaa atccaatatg gaacaagcct tgcttatccg
     4621 atttcgtcca tcacagctca tgtatcagct gtaccaaatc atcagaccga cagatctact
     4681 tcgttgaaaa caagaggaga aacagctatg agcgctgttt ttggttatga actggatttg
     4741 acaaagctgt ctcctgaaga aaaaaagcaa gtaaaagacc aaattatatc ctatcaaacg
     4801 attcgtccgg tgatccagta tggccattat tatcgtttgg cgagtccttt tgaagaaaat
     4861 attgccgcat ggatgtttgt gtctcctaaa caagatgaag ctattgtgtt tttagggagg
     4921 atactagctt cagctcaacc agcgttccat gaagtatatc tgatggggtt agatgatgag
     4981 gcactttatc aggaacagac ctcgaagcgg atattttcgg gggccgaatt gatgacagtt
     5041 ggactttact tccccgattt tcaaggtgat ttccaaacag aactgcttca tttcaaaaag
     5101 ttatgagaga gaaggaaaaa agtatgaaag caaatataaa atggttagat gatccacaag
     5161 tttttagagt gaaccaatca aatgcacata gcgatcatgt ttgttatgca acaagggaag
     5221 aagtagaaac cacatcctcc cttaaacagt ctttaaatgg atcatggcgt ttttgttatt
     5281 cgccaaatcc ttcggtacga ccaaaaacat tttataaaaa agattttgat gcctgttctt
     5341 ttgatgaaat cactgttcct agtcatattg aattagcaaa ttatgacaag atccattaca
     5401 tcaatacgat gtatccgtgg gagggacatg tatttcgccg tccccctttc acgacaagcc
     5461 ctgagaaaga ggcggaaggg atcttttcag ctgctaaaga caatccggtt ggttcgtatt
     5521 tgacgacttt tcgattagaa gaaggtctaa agggaaaaca aatctcgatt cattttgctg
     5581 gtgtagaaca agcaatgttt atttggttga atggtcattt tatcgggtat gccgaagatt
     5641 cgttcactcc gtctgaattt gatttaacac cttatttgga agaagaaaat attctagctg
     5701 tggaggttta caaaagaagt acagctgcat tcttggaaga tcaggacttt ttccgatttt
     5761 ttgggatctt cagagatgtc ttcttagttg ctcgaggaca aaaccattta gaagatcttt
     5821 ggatcaaacc tcgcttagat gacaaatgtc aaaatggcaa acttgaagta gatttgtctt
     5881 ttgagcaagt agccaaaaac agtcagctga atgtagcgat actagatcct gatgggaagg
     5941 aaatctatca acagcaatat ccgttaaaag agcaaatgac gattcgcatc gatggatttg
     6001 aaaatgtggc actgtggggg cattttcagc ccaatttata taagcttgaa ttggagacga
     6061 tagacgaaga aaaaagagta acggagtata ttgactattc cttcggtttt cgtaaaatcg
     6121 aaatcaaaga taaaatcatc tacttcaatg gaaaacggat gattatcaac ggggtaaatc
     6181 gacatgagtg gcatccgaaa aaaggacgcg ccatcactat agaagatatg gaaaaagatt
     6241 tggctatcat aaaaaatgca aagatcaacg gcgtacggac atctcattac cccaaccaga
     6301 tcacttggta ttatttatgc gaccaagcag gaatccatgt gatggcggaa acgaacctcg
     6361 aatcccatgg ctcatggcaa aaaatgggcg catttgagcc ttcttacaac gtaccaggtg
     6421 cagtacctca gtggcaggcg gctgttcttg atcgagcaaa aacgaatttc gaaacattaa
     6481 aaaaccatcc aagtattctt ttttggtctc ttggaaatga atcttatgcg gaagaaaaca
     6541 ttcgacaaat gaacgattac ttcaaaaaag tagaccccga tcgacttgtc cattatgaag
     6601 ggaattttcc aaatccagca ttcgaagatt cgatttcaga tgtaaaaagc tgcatgtatg
     6661 cgccaccaga agagatcatt gactaccttg aaaataaagc ggaaaaaccg ttcatcttat
     6721 gtgaatatat gcatgacatg gggaattcgt taggtggaat ggattcttac atgacgttgc
     6781 ttgatcgtta tgaaatgtac caaggaggct atatatggga ttttaaagat caggcgatcc
     6841 aagtgactga tccaatcaca ggcagagaag tgatgcgcta tggcggtgac tttgacgata
     6901 aaccaagtga ctatgaattc tcagggaatg ggatcgtttt tgcagatgga caagaaaaac
     6961 ccgccatgca ggaggtaaga tattattatg aaaaatacag taaataaaag tcatatggat
     7021 acggaaaaag ttgcaatcgt cttcggcgac tgtacattag gtgtcaaatc ggggaatacg
     7081 cattatattt tttcttatac aagaggcgga ctggaatcgc tcaataaaaa cggaaaagaa
     7141 tggctatacc gggaaacact tccagttttt tggcgagcaa cgaccgataa tgacagaggt
     7201 agcggatttg gatttaaatc cggtatgtgg ttaggagccg gattgtatcc aaaagtcaaa
     7261 gaaatagaag tactggtgga caataaaaag atcgatttgc cgattgctcc agtaaataat
     7321 caatattcaa gtaatgaata cgctgaaacg gttgaaatca tttttacact ggaaatcaac
     7381 acagtaccaa aaacccaagt gacaatttcc taccaaatcg ctggagaggg taaaatgctt
     7441 gtcaatatgc attattatgg acaaaaagga ttaccggaat tacccgcatt gggtttaagg
     7501 ttcattatgc caacgaaagc aaaatttttt gagtatgaag gcctatcagg ggaaacatat
     7561 ccggatcgga tggctggtgg tgtcgaaggt atttataaag tggcaggact ccccgtttcc
     7621 ccataccttg ttccccaaga atgcggcatg catatggaaa cgaaatgggt agaactaaca
     7681 agaaatcaaa cgttggataa tacggataaa gatcaagaag atttttcgtt gcgatttgaa
     7741 cagcaggata cgccatttgc tttttccgct ttaccattca aagcagaaga actggaacag
     7801 gcaacacata tagaagaact accgccagaa agacgaacgg ttcttacgat ttttgcaaaa
     7861 gtaagaggag tcgggggtat caatagctgg ggagcggatg ttgagtcacc ttaccgaatc
     7921 agcagcgaag aggaccatga aatcggcttt attattcagt agcaatcttt agattattat
     7981 ttgaaaaacg gagtttacaa gacaaagctg ctttattcta tatgtcatga acaagagagt
     8041 aaaaaagttc tcagatactt ttgttcatga ctattttctt caataaaacc aattgttatt
     8101 tttctgaaaa acaaagttaa ctatcagaaa attaaaaaaa gtttcataaa aa
//
LOCUS       DAZUMM010000101         8068 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000101 DAZUMM010000000
VERSION     DAZUMM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.104"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..884
                     /locus_tag="ACG8E6_002371"
     CDS             <1..884
                     /locus_tag="ACG8E6_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFS5062749.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            932..3061
                     /locus_tag="ACG8E6_002372"
     CDS             932..3061
                     /locus_tag="ACG8E6_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFS5062750.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3036..3725
                     /locus_tag="ACG8E6_002373"
     CDS             3036..3725
                     /locus_tag="ACG8E6_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062751.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4133..4819)
                     /locus_tag="ACG8E6_002374"
     CDS             complement(4133..4819)
                     /locus_tag="ACG8E6_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5062752.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4955..5149)
                     /locus_tag="ACG8E6_002375"
     CDS             complement(4955..5149)
                     /locus_tag="ACG8E6_002375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062753.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5199..5639)
                     /locus_tag="ACG8E6_002376"
     CDS             complement(5199..5639)
                     /locus_tag="ACG8E6_002376"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFS5062754.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5643..6488)
                     /locus_tag="ACG8E6_002377"
     CDS             complement(5643..6488)
                     /locus_tag="ACG8E6_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFS5062755.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6637..8055
                     /gene="pepV"
                     /locus_tag="ACG8E6_002378"
     CDS             6637..8055
                     /gene="pepV"
                     /locus_tag="ACG8E6_002378"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFS5062756.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2481 a         1515 c         1714 g         2358 t
ORIGIN      
        1 gtatttcagt accggttgca ctgttctcat ttatgggctc gattatggct ggaatctttg
       61 ctaatatcgt caatttcaaa caatatcaaa cgatttatgc tggtttcaat ccagcggctg
      121 aaagctatac ttataatgac tatttccaaa ttggtatgat tgggatgatt gtaagcttag
      181 ttgtggtact aacggtcgct aatatatcaa tgaacaaaaa gaaacgctat gcaatggcag
      241 caaacgtacc agctgaaggt ggcgacgcac caatgatctc atggttagcc gtattattac
      301 cagttttagg ggtcgttctt ttagatcttc aaatcatctt aggttttatt ctagcaggta
      361 tctgggcatt gctgtttact ggaaaattgc gcggaggtta taaagaaatc tgtcgtcagt
      421 ttgcgaaatt atttacagat ggcgcagtcg atgttgcacc aatggttggt ttcttgatga
      481 ccttagcgat gttcaataat tcagctgcgt atgcttctcc ttatttttca gctatttttg
      541 gagattggat accgcaatca ccattagtct tggcaatcgt atttgctata ttgacaccac
      601 taggattttt ccgcggtcca atgaatctag tcggttcagg ctcagctatt ttagctgtcg
      661 tgttagctgt taatccaacg atgtcgccag cctttttgtt cccgttattt gcaattacaa
      721 cgattgctcc tcagcattta gatattacac aatcttgggt tgcttgggga ttaggttata
      781 caaaagtaac ttctcgagaa tatatgaaga aatccatccc aactggttgg atcatcggtg
      841 cgatcttatg cctgatcacg ttcttattgt acgggaacgc ctagtaaaca aaacgtcgat
      901 aaaaacgatt agataaatgg aggaaaagaa aatgaaacgt tttgtgcgaa tgggaattga
      961 tgttggcggt acacatacga aagcagtcgc aatcgataat gcgacgcacg aaatcatcgg
     1021 aaaatcttca gtgaaaacaa cccacgatga tgtccgtgga gtagctgcag gtgtcgttca
     1081 gtcttttcaa aattgtttgc gggaaaataa tatcagtcca gaagatgttg tgtttgttgc
     1141 acattctacc acacaagcga ctaatgcact gattgaagga gacgtagcaa aagtcggcgt
     1201 aatcgggatg gcaaaaggtg gattagaagg ttttttagca aaacgacaaa ctagattgaa
     1261 cgatatcgat ttagggaata agaaaaaaat agagattgtc aatgcattct tacctgtcaa
     1321 aaacttaaat gtagaccgtg tttcagaaac gatttcttct ttagaaagag aaagagcgga
     1381 ggtcttggtt tcttctatgg catttggagt agataatggg gaaccagaac gagttgttta
     1441 tgaagcagca tctgtgaaag cgatccctac aacgatggct tctgacatca caaaattata
     1501 tggattgaca agaagaactc ggacagcagc aatcaacgca tctattttac caaaaatgct
     1561 agatacggca acctcgacag aagactccgt acgagaagca ggggtcaatg tttctttgat
     1621 gattatgcgc ggagacggcg gagtaatgga aatcaatgaa atgaaaaaac gacctgtttt
     1681 aaccatgctt tccggtcctg ctgcatcagt catgggctct ttgatgtact tgagagcgtc
     1741 taatggtgtt tattttgaag taggcggaac aacaaccaat atcggggtca ttaaaaatgg
     1801 acgtccagca atcgattatt caattgtagg tggccaccca acatatatct cttcattaga
     1861 tgttcgggtt ctaggtgtgg caggcggttc gatggtacgg gctaatcagt caggaatcat
     1921 cgatgtcggt ccacgttctg cacatattgc cggtctagat tatgcagtat ttacggagac
     1981 agaaaaaatc aaaggtccta aggttgaatt tttctctcct aaagaaggag accctgctga
     2041 ttacgtcaag gtagtgatgg aagacggaga agaagtgact attacaaata catgtgcagc
     2101 caatgttctt ggattagtgc aagaagaaca tttttcttat ggaaatgtcc catctgcacg
     2161 aaaagccata caggcattag cagactattg tcatacgacg gttgaagaca tcgcagagca
     2221 gatcatggaa aaatcctatg ctaagattga accggtgatt ttagaattgg cagacaagta
     2281 tcacttggag aaagatcaga tttctcttgt aggtgtaggg ggcggcgctg cctcattgat
     2341 tacttacttt agtaataaaa tgggtgtgaa atattcgatt cctgaaaacg ccgaagtgat
     2401 ttcttctatt ggtgtagcac ttgcaatggt ccgagatgtt gtagaacgta tcatcccttc
     2461 gccaagtaaa gaagatatac gatcgttgaa aaatgaagcg atgaataaag cgattgaatc
     2521 aggcgctaca ccagaatcga tcgaagtcca cgtagaaatc gatccacata cttcaaaagt
     2581 aacggctatc gctactggtt ctacggaagt gaaagcaacc gatctaacca aggagatcac
     2641 aacagaagaa gcactagagc ttgctgcaga ggatatgcga ttaaataaaa atgaagtttg
     2701 cttattggaa aatacacctt tcttttatgt atgcggcgaa cagaatcgtt caaaaaatgc
     2761 tggaagtctt cggatcatcg atcaaaaagg atttatcaag gtccaacgtg gacatgcttc
     2821 atgtatgaaa acaactgcag ccaattatat gacagctgtc gaacaacttt gggaagatat
     2881 ggcagtttat caaactgaat tgattgcccg accagaattt tatctctgct taggagcaag
     2941 agtctctgat tttacagcga cggatctgga acaactacag cttttgatgg atctggaagt
     3001 atctactatg gaaccggagg aagaagtgat cgtcgttgcc ggaaatatca aacaaacata
     3061 agttagaaga gttactaaac gaattacaac agatccccga agaaatttgg ggattctacc
     3121 agtttcggcg agatttattt tggaaaaaga ttcctttgtc caagcagaaa attcttatac
     3181 aacaatcaat cgattgtggt atcgaaacgg catgtagcat caaaaaaaag tacccttttg
     3241 ccgatgtagg agaaatctgt gaacaaatgg ctgttccaat cgtttcctgt gaatcagagc
     3301 aaataaacga acgaatcact tttgcaactt atgcagaaga tgaagggatc cgattgatga
     3361 cggagccgtt agaaaaatta aaatgttcag gactagcttc gatctctaaa gaaacagcgc
     3421 aagctttgat tattggacat gaactatttc accacatcga agcttctgtt aagggaattt
     3481 atacgcaaag cgaaaaaatt gtgctgtggc ggttgccttt ttatacacat caatcaaaca
     3541 ttcgtgcatt gagcgagata gcagcgatga gtttttcaaa agagatgaat caaagcgatt
     3601 tttcgcctta tgtattagag gcagtcttac tttggccata taatgaaatt cgtagccaag
     3661 ggatcttaga agaaataaaa gaaatagaaa aaagatgtac ggagtatgat ttagattaca
     3721 aataaagaga ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata aacggtgttc
     3781 tcctgtcaac tcctcggtca caagtcaaaa tattcaacaa acatgagaag ctgttttttg
     3841 aaattgttcc ttgtgactcg gagatgaacg gcaggctcac gacctcttgg aaaaaggtgt
     3901 tcaaaagctt gcatctgcgg taataagaac agccaagtta aaaacataag aagctatttt
     3961 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct caataaacgg
     4021 tgtgcaatca gctgaccctc ggtattagct caaaaaaaac ggcaaaatat gaggaactat
     4081 tttgccgttt atttctctaa tactcaggtc aaaacgctga ttccaaaacc tcttatttga
     4141 tgggtctgtc ctgaatatct tcaaaataat cggggtaatt atgaatcacg tattcttgct
     4201 cctcgataat catatgtaaa tgcatcgtgc aaagaaatcc gacttcatca aagttatgat
     4261 caatcattga ttgaagcatt gtctgatgtt catctaatac tcgaccccag tctaattctg
     4321 aaatcgtcaa acgtaaccat ctgaaacggt tcaagtgcgt actataactt tctagccaat
     4381 cccaaatttc tcgttttcca gcaatttcat aacaagttcg gtgaaaatca gtatccaaat
     4441 caaaaaaagc ttttttattt tgagcgatca ctgcctgatc ggtttgttct agtatttttt
     4501 ctagtttagc ttgatcttgc ttggtcattt tcgcactgca ttcttgcagg atcggttttt
     4561 ccaacttttc ccgcgtataa cgggctaatg aagcagatcg gatatcgatt tttgaaacat
     4621 aagtgccgga ctgtggaatg gtttttacaa gctcttgatt gcgcagttgg atcagtgctt
     4681 ctcgaatcgg tgtacgcccg atagctagtt gttcttctaa cactttatcg gagattttac
     4741 ttccgggtgc taactcggaa taaatgattt gtttacgtat agattcataa gcttgctgtt
     4801 gcaaattatt gataaccatt taaccttcct ttctatgcag atataatcta ttataacata
     4861 aatcgcttac aaatcgtgtg ggcataaaaa cacagtctgc aaaaaaagcc ctgataggca
     4921 tgcctattag agcctcgaaa agaaaaatat tgaatcaatc tggtcgtacc tcttggatcg
     4981 gtttgacacg accgccgatt cttttagcaa aagcttcgga ttcttttttg tctttgaaaa
     5041 tgagcagatt gtctaagttt ttttcccgag tgcgacagcc agttttatcg atgtaaccgt
     5101 gcattacttt aacgacatac atggggatca cctgcttttt tcttttcatt atacgaacta
     5161 ctcgacacaa tacaagaaaa atgcttagac ggtctttatt aagaatttac atcatccaaa
     5221 gaagcgtacg cttggaataa ctgttctaac tccggattga ctttgctcag cttcactaat
     5281 cgttgcgaat cagaaagtaa aaaacagtgt ttactactgc cagtagtttt taattttttg
     5341 tcttcagttc ccacaatttc gtagctaaaa tccaaccgtg ttcccgtata cttttggata
     5401 gttgggtgga tgctcacttt ttctccgtaa tggatcattt ctttatagct gcaatttact
     5461 tccagaacgg ggatgatgat cccagattct tcgatttttt tatagggaaa ccctagatgt
     5521 accaataagg ctacccgagc ctcttcaaac cagcggatat aattgctgtg atgaataatc
     5581 cccatttgat ctgtttcata ataaaaaggt tctcgcacat aacctgtaaa tttctccatg
     5641 actcatgcct cgttttccat tgatttttta caaaaataat tcatccactt cggaagtgct
     5701 tcgctgattt gtgtcggcaa aacaatatat tgttcttttg ccagctggtc tccaatgaaa
     5761 ctatgaagat aaacagctgc tgcaatcgtt tggtcatttt gaggaaactg ggctaaaaat
     5821 cctgtgatca tcccagctaa agtgtcaccc atgccgcctg ttgccatagc agcagaacct
     5881 aaaggattgg tgtagaagtg ctggtgacca taaatttttg tctcatgact tttcaaaacg
     5941 agcacagcac cgatttcctg ttgcttcttt ttgttttctt tatcccgttg ttcttgtatc
     6001 ggtagagaac tcaaccgttg ccactccatt tgatgaggtg taagtacagt ttgttcggga
     6061 tagggtagca aaagatgatt agcaacaagt aaacttaaag cagagccatc aatcaccagc
     6121 cattgttctt ttgattggtt ttctagtact tgtttgaaac gttccaaact cttagaagaa
     6181 gtacccaatc cgggaccaat caagagtaca tctgcttgct ttattacgga aagaaactgg
     6241 tcagtatccg tccaatctac aatcattgct tctggcagat gactgtgaag agcactccga
     6301 tttttttcat cagttactac agtcgtgaga cccgctccac tgtgtacggc agctaaagca
     6361 ctcataatga ttgctccgcc atattgttca ttgccgccaa tcaaaacgat acgtccaaat
     6421 gttcctttat gtgaaatctt tggacgaggt gtgatgactc ttttcactag ttgttcgttt
     6481 atcagcatcg aaaaacctcc ttttactcat tatagcgtat atttttttta gagaaaaaca
     6541 gataagaaaa ggaattgtca gattttttga actatttttt tctgtaattt gtggtatcat
     6601 gaacacgaag acttaaataa gtggaggatt aactttatga caattgattg gcaaaaagaa
     6661 gtagaagcac gtaaagaaga cttgcttgaa gacttgaaaa atcttttacg tgtcaacagc
     6721 gagcgcgatg attcaaaagt aacaccagat gcaccatttg gacctggacc tcgcgacgca
     6781 ttgaaacata tgttagctta tggcgaacgt gatggattta aagtaaaaaa tgtcgataac
     6841 tatgcagggc atattgatct tggcgaagga gacgaaacgc tagggatatt tggacacatg
     6901 gatgtcgttc ctgcaggaga tggctgggac acagatcctt atgaaccagt aatcaaggat
     6961 ggaaagatct ttgcacgcgg atcaagcgat gacaaaggtc caagtatggc agcttactat
     7021 gcgatgaaga tcattaaaga acttgacttg aaactatcaa aaaaagttcg cttcgtagtc
     7081 ggcagcgatg aagaaagcgg ctggggcgat atggcttatt atttcgaaca tgaagaagaa
     7141 ccagattttg gtttctcacc agatgctgaa ttcccaatca tcaatggtga aaaaggaaat
     7201 gtttctttag ctttacgctt caaaggcgac aatgcaggag attatgtctt gaaatctttt
     7261 gtttccggac ttcgcgaaaa tatggtgcca ggaacagcaa ctgcagcact tcaagtacca
     7321 agtgcagatg cagctatcgc gatggaagaa gcattttacc aattcatcga agcaaatcca
     7381 gtaagcggaa cgattgaagc ggacaatacg tatgtaaaaa tcgaattagt tggtaaaggc
     7441 gcacatggtg caagcccaca atctggtatc aatgcaggtt ctttcttggc attgttctta
     7501 gataactatg aattcctagg atctgctaaa caatttatcc atgtagcagc tgcttatgtc
     7561 catgaagatt tttatggaga aaaactagga gtcgcttatg aagatgaaaa aatgggcaaa
     7621 ttgacaatga atgctggact atttgctttt gaagaaaatg gtacagagga agcgaacttc
     7681 atcaatatga atttccgttt tccaaaaggt gtaacagtag acggattgca aagtgacatc
     7741 gaacaaacag tggggcaaga aggcgcaaca gttactcgag gtgctcgcgt aatggaacca
     7801 cattacgtac caatggatga tccattggtg gctacattat tgcaagtcta tgaagaccac
     7861 actggagaaa aaggctacga acaaatcatc ggtggcggaa cttacggacg tttgttgaaa
     7921 cgtggcgttg cttatggagc gatgttccca ggatatactg atacaatgca tcaagcaaac
     7981 gaatttatga gtcttgatga cttgttcaga gcaacagcaa tctatgcaga tgccatctat
     8041 cgtttggcga aataaaaata ctaaaaaa
//
LOCUS       DAZUMM010000102         8064 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000102 DAZUMM010000000
VERSION     DAZUMM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.105"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(604..1644)
                     /locus_tag="ACG8E6_002379"
     CDS             complement(604..1644)
                     /locus_tag="ACG8E6_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS5062757.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1993..2319)
                     /locus_tag="ACG8E6_002380"
     CDS             complement(1993..2319)
                     /locus_tag="ACG8E6_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062758.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2306..3094)
                     /locus_tag="ACG8E6_002381"
     CDS             complement(2306..3094)
                     /locus_tag="ACG8E6_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5062759.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3462..3752)
                     /locus_tag="ACG8E6_002382"
     CDS             complement(3462..3752)
                     /locus_tag="ACG8E6_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFS5062760.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3888..4067)
                     /locus_tag="ACG8E6_002383"
     CDS             complement(3888..4067)
                     /locus_tag="ACG8E6_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062761.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4064..4414)
                     /locus_tag="ACG8E6_002384"
     CDS             complement(4064..4414)
                     /locus_tag="ACG8E6_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062762.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4407..5732)
                     /locus_tag="ACG8E6_002385"
     CDS             complement(4407..5732)
                     /locus_tag="ACG8E6_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS5062763.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6182..6796
                     /locus_tag="ACG8E6_002386"
     CDS             6182..6796
                     /locus_tag="ACG8E6_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5062764.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6961..7785)
                     /locus_tag="ACG8E6_002387"
     CDS             complement(6961..7785)
                     /locus_tag="ACG8E6_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062765.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT         2490 a         1432 c         1166 g         2976 t
ORIGIN      
        1 gtaagggatt ttattcttta tttaaaactt tgcaacagaa ccgtccgttg cgggtaatac
       61 cgtaacactg aagggcacgc attaaggcgt catcttaatg ctttcctgta ggacactgac
      121 ggagattacc agtctcccga gccttacagg ggcctttctt ttttttgtat tttcaattat
      181 cacaatttat cgtccaaaag tgcagaacga aaatcaattc atcacaagca tatcagacaa
      241 ttaaatttaa aacaactaaa aatatacaaa aaagtgtaat tgaatattag cagcactgcc
      301 tgaaaagaag taatatcagg cttttcagct tggggaaata agtgaattac ctctcttcat
      361 gaattattat aactcaaaaa agcacatatg caaacactag ttccctattt ttttgaatga
      421 aaaataacta aaatatctaa cgattatgac tttaaaacct acgtttgtga ttgttttcat
      481 aaacttttaa tctaaaaact ccggaataat agctatttat ttgtaacatt tgtaataaaa
      541 ttaaaatcat aaaaaagttc taatttaaaa ttgatcattt ttatagtgat cgctttttat
      601 caactactct agccaatttt ccgtactaac tgttggtaat tcgttaaatg tttctctttg
      661 ttcgtttgca agctttgccg caatagcttt ttcgacaaac caatttttta cataaacgaa
      721 aattagattc tgcaaattgt ttactttttc agttttttgt ttttgataag ctttcagcaa
      781 agtcatatat aatcctttgt cagcatcaat tccataagat tccagttctt cataaacaac
      841 aactgtttgc tctaattctt cagctttttt ctttgcatta tgaatagttt ggacggtttt
      901 ttgagcgtct tcgtaagtac tagaaaatgt ttttatcaag tttaatacat tttctggtac
      961 aaatgtattt atactgtcct ctttcatgat gtcgacgaag ttatctagta aaacttgatt
     1021 ttgtaactga tcagtttcag tgtctattat gtgtctatta gtgtctatag aattagaata
     1081 gaatagattg tctgcgattt tcgcaattac ggttgagtcc ttgatatgac tggattttgc
     1141 cgtatttgcg attttcgcaa ttacggtggt atcattgatt tctcgcggtt ttgccgtatt
     1201 tgcgattttc gcaattacgg tggtatcagt attgtcttcc tcttccatga ttttaaagac
     1261 gtcttgattc gttacttctg gcttgcctaa gtacaataaa ttaggcatgg attcaatctt
     1321 accgtcacgt ttatttactc ttcctctctt ctgtttcaga agacctgctt tttctaattc
     1381 ctttttgatt ttagatactt taccttcgcg gcattgtaac aatttcatta attcttcaac
     1441 tgtgaatatg aaaaatatat tatcatcaga atctacccag ttatttttga ctgaataatc
     1501 aaatcgatct ttaagtagca tataagcaat ttttgcatca tttgatagat ccatgtattt
     1561 ttcgttagta aaaaatacct taggcatttg ataatatttt tcacgataaa tttcttttga
     1621 tttgtaataa ttaaaatcat tcatagccaa tcctctttct gtaggatagc gttttgttca
     1681 aaactatgtt acaatgaatt aaacaaatat atctatccac tatatatttt tctactgctg
     1741 ataagtcgcc aaactttgtt aaagcagtag tcttttttta tcttttttta gtttacctta
     1801 aaaaataaat taaagcaaac cgctctattt caaggtttaa gctcatagta actatatcac
     1861 gatattacaa taatattata tcacgatatc gtaatattac aatatgaaga taagcgactt
     1921 atcagctttg atactactga tttttataaa aaaaatatca cgatattatg atatcgtgat
     1981 attgaatgta aattaaattt tattttgttt ctttgctagt ttatatcctt caatcaagta
     2041 ttcttcaggt atggacttta atagcgcatc ttctataacg tctggtttac ttttgtctga
     2101 ttcagcaata gtcgtccaat atttgagtgc tgacattaga attggattta tacgaatgct
     2161 atctgctgtt cttacactac ttccttgatc ttttatgact ttacttatat ttttcttggt
     2221 taattcgatt tcttctactt cttttgattt aaaattcttt ttatttttat ttcctagtaa
     2281 tctacttact gcagcttgac tgcgattatc ttctgccatc aaaatcacca acttctttta
     2341 aatattcttc tgtaaatgct ttatagtcat cttcagctgt tgagggttta ttaaaattga
     2401 ttagatctga tccagcagct tgcgcttcgt caacggttat tgtcctacga atataagttt
     2461 tgaaaatttt tgcaccaatg actctagaaa tttcggttgc tgcagacgtc atttctttag
     2521 aaatgttata acgcttatta tacttagtaa atagaactcc tcgtacttca agcttgtcgt
     2581 tcgtatattc ttttaccgtt tcaatagttt cacttaactt tattagtcct tttaatgaga
     2641 aatacgcagc ttcaactgga atgataattt catcagaaac agtaaatgcg ttgactgtaa
     2701 gcattcctaa acttggtgga caatcaatta taatataatc atagttgtcc ttgattggtt
     2761 gaagtactct tttaagcctg tgttcacttc ctacttgagt aaattccctc tctgcagaag
     2821 aaagggctat gtcggctggc aaaacatcaa ttcctttata gtccagaata gcatctgtaa
     2881 tgtcagcctc cccttttaat acttcataaa ttgtaggttg gtttgttcca tctatatcga
     2941 aacctacatt atctgtagca tttccctgag gatcaatgtc cactaaaagt acatttttac
     3001 cgtaaacaaa tttcaaacat gctgctaaat taacgctagt cgtagtttta gaaactccac
     3061 ctttttgatt ggctactgac aatattcttg ccattaaaat atcccccatt catcttatat
     3121 tataatataa ttatatcata atattgtgat attgcaatat cgtatatgca ttatattgta
     3181 atattgtgtg attattttgt tttaaagata tatatcggtt cggatataaa gattattcat
     3241 atgttattcg aaattaaaat aatcactttt tattacttta aaatttttta ttaatgataa
     3301 gaaatatgtt ttgcgagcag caactagaat aatttaaaca ttactaaact gtctatattg
     3361 ttactaataa ttcccttctt atagtgcatt ttcttagaaa aaaacaatat tacgatatca
     3421 tgatattaag atatcgtaat attatacaat tgtgattgat attatatatg tttttttatt
     3481 tttttatcat gtttaatctt gaaggaatca tattcttttt ctaaagacag gagcttttca
     3541 taaacatcag acatgtttaa actagaggcc aggcttaaat cataaacgaa tgaagcaggt
     3601 aatgatttta ttctacgttg tcgggttatc cagcttgcaa ccgttccttg tgggtaggca
     3661 tataactcac aaaattgttc tacagtgatt cctaaattct tgataatgta aacattgatc
     3721 gggtgcgagt aaacaaatgt attgatagac atggtgcgta agcctacttt cttttattat
     3781 tgcgtttgca ataatagttt aacacacgca ttattctagt atctactcct atttgtaaaa
     3841 acaaaaaagg attcatagag ataactctat agaatccgta aaatacctta tatatataac
     3901 gggaagatat aaaacctaaa attatgataa cctgctgcag cttctacata gaaagagaag
     3961 cgctcaacac caacctcatt tatcaaacgt tggccataac cccaaatctc ttctgatagc
     4021 tgatcaaagg ttatatactt tagctcatat tgctggataa tgttcattct aatcgactcc
     4081 atttattatc ctggtaaaat tcgatatgac gtatttcgtc gtagtgaatt ttgtcttttc
     4141 caacgtagat ccccagctca tcactaccta agatcttgcc aatgatatct tctggatagt
     4201 atccttcagc gtttcgttct tctttttgaa tagatactga tctatttttt atgactgcag
     4261 cgttcaatac ttccgcaatc tcttcagtcg tcatttgttc ttttgctaaa ttttgactgg
     4321 ttcgttgttc attgtcggca tcaatttctt cagtatgatc agataaataa aaccctgccc
     4381 actttaaacg tccacgatca ttgtaatcaa tcatttttaa ttcccccatt tccgcccgca
     4441 tgaccaccaa caagtgtgct tctagtaatg cttctagcgc cttctaacat tgaagaagca
     4501 tgtacaatcg acgtaaaacc atatttttgc cgaatcttgt caataatttt atctaaattt
     4561 tcttcgtcaa tttgcttttg tggatcacta aataaatcca gctgaagcga atcggtgtac
     4621 acaagcttgc tataggttat tccaacatgc cttacttctt gtccttcata atacttatca
     4681 aacaagaaaa gacaataatt ctgcaattcc tttgtattat ctgtgatagg gatcttcatc
     4741 tggtgtgaaa accctggtct cgtttctaag atcgacgtgc caatgtgtaa atggacacaa
     4801 cctgttttac agttatgctg cctaatgcgt atcgctactt gttccgccat ttcttttacc
     4861 accagctcga tttcatttct tctagtataa tctcgtggta acacttgtga atttccatat
     4921 gatttttcct ttgtcggttc tggtgggatt gctatatctg ttcgatcgat cccattagca
     4981 tggaagtaca gctgtaagcc aatcacacct agtctattct taatcgtata aggattccag
     5041 ttagcaagat ctctaatgct cataatcccc atctgattga gtcttttttt cattcgagag
     5101 ccaattcccc aaaagtccgt catttcagga atattccata ctttttcagg aacgttttcg
     5161 tacgtccatt ccgcaacaaa tccttcatta tgctttgctt cgttgtctaa ggctagttta
     5221 gccaaaaggg gattatctcc gacacctact gtagcaatca atcctagctc ttctttaata
     5281 ttctcctgga tcatttgagc cagcttcttt ctccgttgac ttcgtgttcc ttcagtcgta
     5341 aaaagattca gtgatcgggt cacttttaag attgattcat cgatcgagta aatcagtaga
     5401 tcttcatcag ccacatatct tctgaaaata ttatttacct gcatatttcg cttgatatat
     5461 aggttcatac gtggtggaac aacgtgtagt gttttaggaa atggttgtgg taagtcacgt
     5521 ggtctactca catttgtaat accataccgc ttttttgctt caggagaaga agctaatatc
     5581 aatcctgaac cagtattatc agctcgactc ataacaacaa gttctgttgt taatggatct
     5641 aaatttcttt ctatacattc gacacttgcg taaaagcttt tcatatcgat taaaaagtaa
     5701 tcatttactg gctcttttga ataatccatc ataaataaca ctccattctt tcacattata
     5761 caaacatatg ttcgatattg taaagaagaa atagctaaat acataaaaaa agaagcatag
     5821 gtatgctatc accctgcttc ttcttttgat ttgtatgatc aatttattct atcactttac
     5881 taattaattt gaaaatctaa taaaaactct cactctaaaa gagtgagaga ctgcaaatta
     5941 gagtacggac ttttttggag ttccgttctt taatattaac aaatgaataa aatccttaca
     6001 acatttgaga ataagttatc agtagacact tacaaaaaat tctcatacta tttcctccat
     6061 cattagtttt tgtctatata aggtttaccc taaatagaca ctggtataac attgaaaaat
     6121 atggcttcct cgtgtatgat tagggtgtat tttaaatagt tgcagaaagt aggtaaaagt
     6181 tttgagagtt gcatacatac gtgtctcatc gatcgatcaa aatgaacaac gccagattga
     6241 agaaatgaaa aaattcggtg cagagagaat tttcatcgag aaacaatctg gcgcaacgat
     6301 tactcatcga cctgttttcc aagaagcatt agattacgtc cgagatcaag atatttttat
     6361 tgtggaagct attgatcgac ttggaagaaa ttatgacgag atcatcgctt cagtcaatta
     6421 cttgaagaag aaaaatgtga agctgatcat tactagttta ccgatcatgg ctgaagcaat
     6481 tggcaatcct ctcctggata aattcattaa ggatctgatc atccaaattt tggccatgat
     6541 cgctgaacaa gaacggactg aatcgaaacg tcgccaggca caaggaatta agatcgcaaa
     6601 agcaaacggc gtctacaaag ggcggcccaa attatacagt gcagacgcaa aagatcccca
     6661 acgtcgcctt gtttacaaaa gcatcgttga agatcttaaa aatggtgttg cgattgctaa
     6721 gattgctaaa gactacaatg ttacgagaca aaccgtgtat agaataaaaa aagacagcat
     6781 ggtcaatgac aaatagttga tcatgctgct tttattatct tctcatcttt aatttcctac
     6841 atataattac tatctataaa attaagtctt actcagaaca gataaagcat aaaattccaa
     6901 caaaccattt tttacttaac taaataaaga gtctgttaat ataatcttca cttatttacg
     6961 ttaatcccct actgtatttg ttggttcctt atctatattt tctacaaaat ccttattatc
     7021 ttgtgattta gcttcgtata attctttgta tgtcgacaca ttggttatac taattcctat
     7081 agctagttct tgagactcta cttttctctc tagctccttt gacaaagtga tttgagattt
     7141 atttggttcc gttggagtca attctataga ttcttcataa aaatctctat acataaagct
     7201 atcattaatt ttataaaaaa aattaaatgt ttttagcact gccatataac attcaaaaca
     7261 ttcttctata gttggtttac ttccttgagg agaatgaacc aatttattac gtttttctat
     7321 gaaattttta atttcctttc ttatttcttt cggaaactca atattaaagt accctttaaa
     7381 catgctccct aataatttgt ctacaggggg agattgtaaa ttctcaaata ttagagttaa
     7441 atcaggtttc tcttgttgat aaaattcctt tacccctatt tcacaggctg taattatcga
     7501 aataaaccta tctctagtat ttgatatatt tttacttctt ttaaggagta taagagaaaa
     7561 ggggatactt tcgtgtccat gcatgtaatt aactaattgt tgagcttcag aagttatatc
     7621 tctaattata tagttaacgg aattaggaag tgtctttaac tctaatgaaa tttctatcca
     7681 attactatct tctaatctgt atatgctcga agtataattt gattcgtcag caagcatcgc
     7741 tacatcccaa acactgatca taaaatcttt taaacgttca atcatttcag caatattgct
     7801 aaaatcagct aatttttctt tagaataaaa aaaaacattt atatcagaat caataaattc
     7861 tattttttta ttactatatc gtcatcatta tcttgaccat ttatgtttat tgttccctga
     7921 gtggggagta cttctttcaa attcaagtta aatttatatt ccattacttc tcctccaata
     7981 tataaaaatt acttaactgt atccaaattt aatatgtaaa taaatcatta atttttagct
     8041 aaattctatg agaagtttat tttt
//
LOCUS       DAZUMM010000103         7901 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000103 DAZUMM010000000
VERSION     DAZUMM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.106"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            709..1602
                     /locus_tag="ACG8E6_002388"
     CDS             709..1602
                     /locus_tag="ACG8E6_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5062766.1"
                     /translation="MGKISLGIDIGGTFIKYALIDKQHRIKEKWKVETVKYNTADEFY
                     DYICSNIRNVDEIDKIGVSAPGLIDEDSNVKSYAAPNVAIMYGTNINNEINKRTNKKV
                     SAINDAKAAGLCEFKIGNAKGYKSSAFLIIGTGTGGCICDENGVVYGKDSFAGEFHNM
                     PFVNAEIGGLDKMGDYASITGLVNLYNKKANREEQVQYGNEVCKKYLNGNESAVSSVD
                     EWIGNIVAQLIVITVFYNPEVICIGGGISEENWFINKVRETYKKTCREYLEADFITTE
                     INRCKHNNDANILGAVIKASI"
     gene            1676..3115
                     /locus_tag="ACG8E6_002389"
     CDS             1676..3115
                     /locus_tag="ACG8E6_002389"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5062767.1"
                     /translation="MIYEELDKFQDDFLWGSASAAYQVEGAWDKDGKGESVWDVYTRQ
                     PGTTFKNTNGNIAVDHYNRYKEDVALMAEMGLKAYRFSIAWTRIYPNGRNEVNEEGLK
                     FYENLIDELIANNIQPIITLYHWDLPQHLQDLYGGWESREIIHDFNEYCVTLFKRFGN
                     KVKHWVSLNEQNIFVTLGYVTAMHPPAVKDQKRMLEANHIANLANATVIESFKKYVPD
                     GMIGPSFAFGPVYPFSCDPKDVLSAENAEDLNCNWWLDVYCKGKYPVFAFKYYERLGI
                     APTIEDGDLELLERVKPDFIGINYYQTSTVAFNPLDGVGQSEGINNTGKKGTTKESGL
                     PGVYKNAKNAHLETTNWDWAIDPTGLRIGLRRLTSKYNLPILITENGLGEFDKLEEDD
                     TVNDAHRIDYLSAHIKACKEAITDGVDLLGYCTWSFTDLLSWLNGYQKRYGFVYVDRD
                     ENDQKDLRRIKKNSFYWYKNVIRTNGEEL"
     gene            3163..3465
                     /locus_tag="ACG8E6_002390"
     CDS             3163..3465
                     /locus_tag="ACG8E6_002390"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5062768.1"
                     /translation="MKKILLVCSAGMSTSLLVTKMRNSAKEKGIEVEIDALPVAEAST
                     AVDNVDIVLLGPQVRFQKGTVEKLVKGRIPVEVMDMRLYGMMNGKAILEDALQKIN"
     gene            3484..4848
                     /locus_tag="ACG8E6_002391"
     CDS             3484..4848
                     /locus_tag="ACG8E6_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5062769.1"
                     /translation="MSFMDKFQSAIERLLVPLSTKLNAQRHICAVRDAFILSFPLTMA
                     GSLMVLLNNVFLSADGFMFKILQFDKLFPGIVKFQAVFAPVVKGSADIFSILIVFLIA
                     RNLAKLMEGDDLLTGLTAISVYFIIYPDYFDNEGVNYLTNQYMGAQGLFVAILVGLLV
                     GELMSRLAKSDKLEIKMPEQVPPAVARSFKVLIPIIITTVFFSVLNYFVKMAAPGGLH
                     ELIYNIIQTPLTRMSQSLFSVIILALLSQLLWAMGIHGPNTIAAIRDTMFSEAGNANL
                     LHYAESGTTWGAPFPITYSGLATAFAEYGGSGATLGLIIAILIFSKNKESKSIGKLSF
                     VPGLFNINEMVIFGLPIILNPIYIIPFIIAPLVNIMIGYTAVMILKIMPPVTIGIPWT
                     TPGPLMPFLGTGGNIVALFVGFICLAVSVLIYSPFVIAANKAAIIEDEMENEIQSQEV
                     VESI"
     gene            4904..5218
                     /locus_tag="ACG8E6_002392"
     CDS             4904..5218
                     /locus_tag="ACG8E6_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5062770.1"
                     /translation="MEGIELTAFSIISNVGMAKSLAIEALRDARKGKYNEAEKKMAEA
                     SEYFVKGHHAHTSLIQKEAAGEKVELSLIIMHAEDQLMSTETTKLLIEEMIEMFKQLN
                     NK"
     gene            5552..6598
                     /locus_tag="ACG8E6_002393"
     CDS             5552..6598
                     /locus_tag="ACG8E6_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5062771.1"
                     /translation="MMNSTEIAKIAGVSRSTVSRVINNYSNVSEETREKVLRVIKEHE
                     YVPHASARMLAGSQNKVIGLFIIDLIHEEVGLKNRITKSPYYLEFTSSVIETASEMDY
                     LVLVHIIHSVAGYEKIKESFYNKTISGGIFIGQNDDDESIKTIIDRGYKVVLLDQSIR
                     PEDATYNQCMIVNADNYNGAYCATKYLIDKKHKQIAHITGGTVKFSSKDRIRGYKKAL
                     EDAGIEVNKNLIVNSEFVENAGYEAAKKLFNGNSEFSAIFASNDKIAFGAIKAIQERG
                     LKVPDDISVIGFDDIESSKYFNPPLTSMKMKLGEMADIATKSIVASIEGETKPSINVV
                     PVSLVERESCGILQ"
     gene            6957..7202
                     /locus_tag="ACG8E6_002394"
     CDS             6957..7202
                     /locus_tag="ACG8E6_002394"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062772.1"
                     /translation="MRASLLIQGQISYFFIGQGGLVRFSYIKFTKDANHAEKVKSLSS
                     NTFGWGIKLFLLKLFEQGIAKIEATFVEAQKNMGCFT"
     gene            7210..7707
                     /locus_tag="ACG8E6_002395"
     CDS             7210..7707
                     /locus_tag="ACG8E6_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062773.1"
                     /translation="MKQRIGNNLALIYSGALLIQDILGYPLRLEHIQTVLINSYEQLI
                     VDTSLGSEQTVFDRVKTLLVMNSHKFLTNSDNKHTPSTIWGKVSIKKNGDIKVNIFPI
                     PFEDLLRREFQVKDLTYLFRQLLDSGNMCAEKGRRTKRIHMNRQVLPTYEILLPSSLK
                     SYFRL"
     gene            7741..7901
                     /locus_tag="ACG8E6_002396"
                     /pseudo
     CDS             7741..7901
                     /locus_tag="ACG8E6_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312651.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         2756 a         1218 c         1643 g         2284 t
ORIGIN      
        1 gcaaccggag tttcttgatg aaaatatact tctatcatta caagctcgtt tggtgtaaga
       61 tgggtataag tcatttatgt tcactctcct tgtatgcttt agcgggtatt acgatttgag
      121 tgtaacataa atggcttttt tatttgtctc gcttaattat acaaacggcg tcattagaaa
      181 atgatggtaa attatttgat ggtaacggtg atgtaacaac ggtaatgaaa ttattgcttt
      241 aacagtttag cgtgataaaa atctatacct tcttaagttg atttattaac ttgagaaggt
      301 atttttttct gaaaaatgat aatgaaaatc aaaatgttca tttctatcaa ttttgaagtc
      361 aaaaaaataa agtacagccc agaaatttgc tctgtacttt aaaaaactag ttaaatgaag
      421 atgaaatcgc tcccttattt tttagtattg gtggaattaa aaggtacttt ttaaacaaaa
      481 actacacata aaaaataaaa aaaataaaaa aagtattgaa atgtaattgc tttcagtata
      541 tgctatatct ataaacgcgc gttcatggga tgttcatcgt ataaaaaaag gtggaaatga
      601 gcgtatcgtt tttcttttta acaaatcatg aacgcgcgtt tatgagtttg aatcaagctg
      661 caatataaga aggcaaactg caagaccttc attttaagga gaagtgttat ggggaaaata
      721 agtttaggaa tagacatagg agggacgttc attaagtatg cattaataga caagcaacat
      781 cgcatcaaag agaagtggaa agtcgaaacg gttaagtaca atacagccga cgaattttac
      841 gattatatat gttcaaatat aagaaatgta gatgaaattg acaaaatcgg tgtaagcgca
      901 cccggcctta ttgatgaaga ttcgaatgtt aaatcctatg ctgctcctaa cgtagctata
      961 atgtacggca caaatatcaa taatgagatc aacaaaagga caaataaaaa agtgtccgca
     1021 atcaatgatg caaaagcggc aggtttatgt gagtttaaaa ttggaaatgc aaagggatac
     1081 aaatcttcag cgtttttgat tataggtaca ggaacaggcg gatgcatatg cgacgaaaac
     1141 ggagttgtgt atggaaaaga ttcgtttgcg ggggaattcc ataatatgcc ttttgtaaac
     1201 gctgagatag gcggccttga taagatgggt gattatgctt cgataaccgg attggttaat
     1261 ttatacaata aaaaagctaa tcgggaggag caggttcagt atggaaatga ggtttgcaag
     1321 aagtacctaa acggaaatga atcagctgtg tcttcagtag atgaatggat aggaaacata
     1381 gtggctcaat taatagtaat caccgttttt tataatcctg aagtgatatg catcggtgga
     1441 ggaatatccg aagaaaactg gttcatcaat aaagtaagag aaacgtataa gaaaacctgt
     1501 agggagtatt tagaagcgga ttttattact accgaaatca atagatgcaa gcataataac
     1561 gatgccaaca tactgggtgc tgttataaaa gcttctatct aaaatacaca acagttttgt
     1621 ttcaagaaag aatgtttgaa aataaattta tattgaaaac gaaggagaag attcagtgat
     1681 atacgaagaa ttagacaaat tccaagatga tttcctatgg ggatcggcat ctgcagcgta
     1741 tcaggttgaa ggagcatggg acaaagatgg aaagggggaa agtgtatggg atgtgtacac
     1801 ccgccaaccg gggacaacat ttaagaatac aaacgggaat atcgcggtcg accattacaa
     1861 tagatacaaa gaagacgtag ctttaatggc tgaaatggga ttgaaagctt atagattctc
     1921 aattgcatgg acacgtattt atcccaatgg aagaaatgaa gtaaatgaag aaggcttaaa
     1981 attttatgaa aacttgatag atgaacttat cgcgaacaat atccaaccaa tcattacgtt
     2041 gtaccattgg gatttacctc aacatcttca agatttgtac ggcggatggg aatcaagaga
     2101 aatcattcat gattttaatg aatactgtgt aacgttattc aagagatttg ggaataaagt
     2161 taagcactgg gtatcattga atgaacaaaa tatatttgta acactaggtt acgtaaccgc
     2221 tatgcacccg ccagctgtta aagatcaaaa gagaatgctt gaggcgaatc atatagcgaa
     2281 cttagctaat gctaccgtaa ttgaatcctt caaaaaatat gttccagacg gtatgattgg
     2341 accaagcttt gcatttggac cagtttatcc attcagttgc gatccaaaag atgttttatc
     2401 cgcagaaaat gcagaggatt taaattgcaa ttggtggctc gatgtttatt gcaaaggtaa
     2461 atatccagtg ttcgctttta aatattacga aagacttggg atcgcaccca ctattgaaga
     2521 tggagatctt gaattgttgg aaagagtaaa accagatttt atcggaataa attattacca
     2581 aacaagcaca gttgcattca atccgcttga tggagtaggg caatcggaag gtatcaataa
     2641 cacaggtaaa aaaggcacca ctaaagaaag cggattaccc ggagtttata aaaatgcaaa
     2701 aaatgcacat ctggaaacaa caaattggga ctgggcaatt gatcctactg gtttaagaat
     2761 tggactaaga agattgacca gcaaatataa tcttccaata cttattacag aaaacgggct
     2821 tggagaattc gacaagcttg aagaagacga cactgtcaat gatgctcaca gaatcgatta
     2881 cttgagcgca catatcaagg cttgtaaaga agcaataaca gatggtgttg atcttctagg
     2941 ctactgcacg tggtcattta cagatttgtt aagctggttg aatggttatc aaaaacgcta
     3001 tggatttgtg tatgtggaca gagatgaaaa tgatcaaaaa gatttgagaa gaatcaaaaa
     3061 gaacagcttc tattggtaca aaaatgttat tcgcaccaat ggagaagagt tataagaatc
     3121 gttcacaatc aatgaatatg aaaaaacaag gagagataga ccatgaaaaa aattttacta
     3181 gtatgttcag caggaatgtc cacaagtttg cttgtgacca aaatgagaaa ctccgcaaag
     3241 gaaaaaggaa ttgaagtaga aatagatgct cttccagttg cagaggcaag tacggccgtt
     3301 gataatgtcg atatcgttct tttaggacca caagtgcgtt tccagaaagg gaccgttgaa
     3361 aaacttgtaa aaggcagaat tcccgtagaa gtgatggaca tgcggctgta tggaatgatg
     3421 aatggaaaag cgattttaga agatgcttta caaaaaataa actaggaaag gaggctaaaa
     3481 aaaatgagtt ttatggacaa atttcaaagt gctatagaaa gattgttagt gcctttatct
     3541 acaaagttga acgctcaaag acacatatgc gcagtaagag atgcttttat tttatcattc
     3601 ccattaacga tggcaggatc attgatggtg ttattgaata atgttttctt gtcagcagat
     3661 ggattcatgt ttaaaatctt gcaattcgat aaactttttc cgggtattgt taaattccaa
     3721 gcagtgttcg ctccagttgt taaaggttca gctgatattt tctcgattct gattgtgttt
     3781 ttgatagcca gaaaccttgc gaaactaatg gaaggcgatg atttgttgac aggtttgacc
     3841 gcaatctctg tttatttcat catctatcct gattatttcg ataatgaagg agtaaattat
     3901 ctaacaaatc aatatatggg cgcacaaggg ttgtttgtag caattttggt tgggctcttg
     3961 gtcggcgaac taatgtctag attagcaaaa tcagataagt tagaaattaa aatgccagaa
     4021 caagtacctc cagcagttgc aagatccttc aaagtgttga tcccgattat catcacgacg
     4081 gttttcttct ccgtcctcaa ctactttgta aaaatggctg caccgggcgg attacatgaa
     4141 ttaatttata acatcattca aacaccattg accagaatga gtcaaagttt attctcagtg
     4201 attattttag cattattatc tcaattgcta tgggctatgg gaatacacgg accaaatacg
     4261 attgcagcta ttcgagatac catgttctcg gaagccggaa acgcaaacct cttacattat
     4321 gctgaaagcg gtactacatg gggagcgcct tttccgataa cgtacagtgg tttagctact
     4381 gctttcgctg aatatggcgg ttcaggagcg acattgggtc taatcattgc tatattgatt
     4441 ttctcgaaaa ataaggaatc gaaaagtatt ggcaaacttt cttttgtacc cggtttgttc
     4501 aatataaacg aaatggttat ctttggttta ccgattattc tgaatccgat ttacattatt
     4561 ccatttatta ttgcgccact ggttaacatt atgattggtt ataccgcagt tatgattctt
     4621 aagattatgc cgccggtaac gattggtatt ccttggacaa caccaggacc attgatgcct
     4681 ttcttgggta caggcggaaa tatcgtcgca ctgtttgtcg gattcatctg tcttgcggtg
     4741 agtgtattga tttactcgcc gtttgtaatc gcagcgaata aagcagcgat tattgaagat
     4801 gaaatggaga atgaaataca gtcacaagaa gtagtagaat cgatttaaaa aagctgcagt
     4861 acgctgagaa caaaccgaaa gattcaagat aggagattta cacatggaag gaattgaatt
     4921 aactgctttt agtataatca gtaatgttgg aatggccaaa tcattggcta ttgaagcatt
     4981 aagagatgcc agaaaaggaa agtacaatga agcagaaaag aaaatggctg aagcatcgga
     5041 atattttgtg aaaggccatc atgcacatac ttctttgata caaaaagaag ctgctggcga
     5101 aaaagttgaa ctttcgttaa taatcatgca cgcagaagat caattgatgt cgacggagac
     5161 aactaaatta ttaatcgaag aaatgataga aatgttcaaa cagttaaaca ataaataagt
     5221 gaacatgaaa gagaaaatat tagacgaact agaattgctt gcttacttta caggcagaat
     5281 ctgatgccag cagcaaaaca tagagcaccc aggatgaaat ataatcctgg gtgccttatt
     5341 tttagaacaa aacaccgggg tggtagtttg ttctgcggtt gaaggcattt gctgatggtt
     5401 tctgcatgaa aggtattgcc aaaagtctcc ttttgctgga atcctgaagc aggcagagtt
     5461 tttcgaaaaa cagaaaaaga ttgaaaaaaa tggacaaata atatagtgtt gcagtatgaa
     5521 cacgagttta cgaacgaaag gagatgcctt tatgatgaat agtactgaaa ttgcaaaaat
     5581 tgccggagta tcacgcagta ctgtatccag agtgataaat aattattcaa atgtctcgga
     5641 agaaacaaga gaaaaggttt taagagtaat taaggaacat gagtatgtgc cacatgcttc
     5701 agcaaggatg cttgcaggaa gccaaaataa ggtgatcggc ttatttatta tagacctgat
     5761 acatgaagaa gttggactaa aaaatagaat taccaaaagt ccgtattatc tagaatttac
     5821 tagttcagtg atagaaacag caagtgagat ggattacctg gttcttgtcc atattataca
     5881 tagcgtcgca ggctatgaaa aaattaaaga gagtttttat aataagacaa tttctggtgg
     5941 gattttcata ggccagaatg acgacgatga atcgataaaa acaatcattg atagagggta
     6001 taaagttgtg ctgctcgatc aatcaatcag acctgaagat gccacctata accagtgcat
     6061 gatagtgaat gcggacaatt acaacggcgc ttattgtgcg acaaaatatc tcatagataa
     6121 aaaacataaa caaatcgcac atattacagg cggtacggtc aaattctctt ctaaagacag
     6181 gataagagga tacaaaaaag cattagaaga tgcagggata gaagtaaata agaacttgat
     6241 tgtaaatagc gaatttgtgg aaaatgctgg atatgaggct gcgaaaaaat tatttaatgg
     6301 aaatagtgag ttttcggcta tttttgccag taatgataag atagcatttg gagcaatcaa
     6361 agctattcaa gaaagaggtc tcaaagttcc agatgacatc tctgttatag gatttgatga
     6421 tattgaatcg tctaaatatt ttaatccgcc attgacttct atgaaaatga agttaggaga
     6481 aatggctgat atcgccacaa agtcgattgt tgcatcgatt gaaggtgaaa cgaagccatc
     6541 tataaatgta gtacctgtct cattagtgga aagggagagt tgtgggattt tgcagtagca
     6601 aaacttgctg aattagttgg agctgattat ttgattattt tgattgaggt agacaatgtc
     6661 tgtgtctatg tcgacttgaa aagtaaagta cagcgcccaa aaatgctctg tactttaaat
     6721 ctcagcaaaa acaggaatat ttcccaaaca tctccttctt tgagtaatta tacaaaaatt
     6781 atgcagactt tcttttttca tgataagata atttaaaaag tttttaaacg atttctcctc
     6841 ctctttttct tatatcgggg gagcatattt ttttgtagac taggggatta taagttcagc
     6901 catcaatgtc tagctcccaa gtcctgacct agtgaaaaaa agataaattt gccccattgc
     6961 gcgcttcgct gctcattcag ggtcaaattt cctatttttt cataggtcaa ggcggacttg
     7021 tccgcttttc ttatattaag tttacaaagg atgcgaatca cgcagagaaa gttaaaagtc
     7081 tttcttctaa tacttttggt tggggtataa agttatttct tttgaaactc tttgaacaag
     7141 ggattgcaaa aattgaagca acttttgtcg aagctcaaaa aaatatgggc tgttttacct
     7201 gaacattctt tgaagcaacg cattggtaat aatctagctt tgatttatag tggagctctc
     7261 ctaattcaag atatactggg ctatccttta cgattagagc atatacagac ggtgcttatt
     7321 aattcgtacg aacagttaat cgtagatact agtttaggaa gtgagcaaac ggtatttgac
     7381 cgagtgaaaa cattgttggt gatgaatagc cataaatttt taacaaacag tgacaacaaa
     7441 catacgccca gtacgatttg gggaaaagta tcaattaaaa aaaatgggga tattaaggta
     7501 aatatttttc ctatcccgtt tgaggacttg ttaaggcgag aatttcaagt aaaggatcta
     7561 acctatcttt ttagacaatt gcttgattcg ggtaatatgt gtgcagaaaa agggagaaga
     7621 acgaaacgta ttcacatgaa tcgtcaagtt cttccaacat acgaaattct attaccttct
     7681 tcactcaaat cgtattttcg attgtaattt agaataaacc aaagtatgaa agtgaggcac
     7741 catgttatta agcaactgaa agatattcct attccaatgc ccgctatttc agaacaacaa
     7801 aaaatcggca ctttcttcaa acaactcgac gacaccatta cccttcacca acgtaagtta
     7861 gatttactaa aagaaacgaa aaaaggcttc ttacaaaaga t
//
LOCUS       DAZUMM010000104         7593 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000104 DAZUMM010000000
VERSION     DAZUMM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.107"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(128..418)
                     /locus_tag="ACG8E6_002397"
     CDS             complement(128..418)
                     /locus_tag="ACG8E6_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062774.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(536..796)
                     /locus_tag="ACG8E6_002398"
     CDS             complement(536..796)
                     /locus_tag="ACG8E6_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062775.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(933..1346)
                     /locus_tag="ACG8E6_002399"
     CDS             complement(933..1346)
                     /locus_tag="ACG8E6_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFS5062776.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1802..2287)
                     /locus_tag="ACG8E6_002400"
     CDS             complement(1802..2287)
                     /locus_tag="ACG8E6_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062777.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2426..2641)
                     /locus_tag="ACG8E6_002401"
     CDS             complement(2426..2641)
                     /locus_tag="ACG8E6_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFS5062778.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2865..3665
                     /locus_tag="ACG8E6_002402"
     CDS             2865..3665
                     /locus_tag="ACG8E6_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5062779.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3684..5552
                     /locus_tag="ACG8E6_002403"
     CDS             3684..5552
                     /locus_tag="ACG8E6_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS5062780.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLKKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5545..6036
                     /locus_tag="ACG8E6_002404"
     CDS             5545..6036
                     /locus_tag="ACG8E6_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFS5062781.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6024..6578
                     /locus_tag="ACG8E6_002405"
     CDS             6024..6578
                     /locus_tag="ACG8E6_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFS5062782.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6596..7591
                     /locus_tag="ACG8E6_002406"
     CDS             6596..7591
                     /locus_tag="ACG8E6_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFS5062783.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQTACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2706 a         1212 c         1443 g         2232 t
ORIGIN      
        1 tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata cttcaaaaaa
       61 gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt taatacattt
      121 taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta agagagatca
      181 taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca tcccatattt
      241 cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg atccagcacc
      301 atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag ggaagtaaaa
      361 acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc tcccccacat
      421 tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt gtatgaataa
      481 cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg gaagtttatt
      541 tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat aagccgattt
      601 cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc ttatggataa
      661 aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg aataagtaaa
      721 agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt aagacaatca
      781 aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc gtaaaaacaa
      841 aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa atatataaaa
      901 atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt ttttgtttct
      961 tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt aaaggttcca
     1021 gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga gaccgaatgt
     1081 ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc atgattggcg
     1141 gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc acagccaggc
     1201 atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt tggataactt
     1261 gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc agggaacatg
     1321 tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta ttttttattt
     1381 atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg ttagctttgt
     1441 gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag ggatcattaa
     1501 aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc caaatataga
     1561 aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg accgaaaatg
     1621 aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat tcataagaga
     1681 aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg tataactttc
     1741 tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata atatcatcta
     1801 atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt gacactcgat
     1861 aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa gctgtgaaca
     1921 aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt tgtaattcgg
     1981 aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga agagaaggta
     2041 cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata cttgattggc
     2101 tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta gaagtacttt
     2161 tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca gaattctttg
     2221 ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg taaatccctt
     2281 tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac ttaaacaaag
     2341 aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc atgaagataa
     2401 taactttttt aataattata gctttctaat atctttcatc aacttttcta gtaatcattg
     2461 ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg atcttcgcaa
     2521 aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac aagcctacca
     2581 acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct aaagttttca
     2641 ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga aacattgctt
     2701 atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt tttgctcttc
     2761 cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt ttttagtgag
     2821 cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg gattggttgg
     2881 atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga agaagtatcg
     2941 tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact gaatgccgta
     3001 tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg gacatagaag
     3061 aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc aataatgcag
     3121 gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag tatgaattat
     3181 cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat ttgatgagcc
     3241 aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat ctttcttctg
     3301 aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa gcagcgatga
     3361 acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt gtggtaggtg
     3421 ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa gaagctcttt
     3481 cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga acaagtacga
     3541 tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc tattatcttt
     3601 cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt aaaacaagag
     3661 gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa ttattgaaat
     3721 tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc aaaaccagta
     3781 atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt attgcaaaaa
     3841 tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt tttgatgaga
     3901 tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa agaatcgctt
     3961 atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat ttgtctgaag
     4021 aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa gagatgatca
     4081 atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata ataggagatg
     4141 agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca gagacgaaaa
     4201 cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag acgaatgcta
     4261 gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat agaatctcta
     4321 gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa tatatccaag
     4381 aattcaatga actgatcaat cagttggaag aaatctatga actaactttg agccaatatg
     4441 aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg atgataagga
     4501 aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt cgacgattag
     4561 atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac ctctctttat
     4621 tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt gagatagaaa
     4681 aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta gaagctatct
     4741 tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg gagttagcat
     4801 ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat acgacaggaa
     4861 aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg gatatacaaa
     4921 taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat ttcgctgttt
     4981 ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat ttgactaatc
     5041 tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag cgattagccg
     5101 ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg tatcaagaat
     5161 atttaaaaaa attagtcgct cctttgatca gaggctatta tgtggatgaa gaatttgaaa
     5221 aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa tttgcttttc
     5281 ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta ataactgatc
     5341 aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata cctgaagaaa
     5401 tgacggctga agttgagaat gaattatttg agctatacga tatcctattt gaactcataa
     5461 aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt ttaaaactaa
     5521 tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt tttcgcagct
     5581 tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt caatcaaaca
     5641 tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg gaaaatccgt
     5701 tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga agtcagaaaa
     5761 atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc gggtgaagat
     5821 atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt gacacaagca
     5881 gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga aaaaagtgaa
     5941 caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa aagtacgatc
     6001 aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag ggatttatga
     6061 ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc gtaccagttg
     6121 tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa gaccgagtaa
     6181 cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg ttaccgttct
     6241 tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc cttccagaat
     6301 actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc aatggtgtat
     6361 tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa gggatcgaaa
     6421 ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga ctgctgatga
     6481 actttattgg tggttggata actgatttta ctactggatt tgtatgcaga cagcaaggaa
     6541 tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt tttaaatgta
     6601 tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga ttattaggcc
     6661 gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc caatgatcgt
     6721 agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa catcaatacc
     6781 tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat gtggaattta
     6841 tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg gcccattagc
     6901 acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa tacagccact
     6961 aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata aaaagtacaa
     7021 atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact ggatagcaaa
     7081 aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa gagaagcagt
     7141 agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt tgattggtgt
     7201 gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt tagcaggaaa
     7261 tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat ctcctttgct
     7321 tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag agattgggaa
     7381 aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata ctcaaactgc
     7441 atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga cagtagaagt
     7501 aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag ttatcattgc
     7561 ttgggtagca agttttggat tatatagctg agt
//
LOCUS       DAZUMM010000105         7310 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000105 DAZUMM010000000
VERSION     DAZUMM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.108"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            159..806
                     /locus_tag="ACG8E6_002407"
     CDS             159..806
                     /locus_tag="ACG8E6_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS5062784.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    935..1151
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1199..3844
                     /locus_tag="ACG8E6_002408"
     CDS             1199..3844
                     /locus_tag="ACG8E6_002408"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFS5062785.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4154..5467
                     /locus_tag="ACG8E6_002409"
     CDS             4154..5467
                     /locus_tag="ACG8E6_002409"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFS5062786.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5457..6110
                     /locus_tag="ACG8E6_002410"
     CDS             5457..6110
                     /locus_tag="ACG8E6_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5062787.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6165..6857
                     /gene="radC"
                     /locus_tag="ACG8E6_002411"
     CDS             6165..6857
                     /gene="radC"
                     /locus_tag="ACG8E6_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFS5062788.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            7007..7207
                     /locus_tag="ACG8E6_002412"
     CDS             7007..7207
                     /locus_tag="ACG8E6_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5062789.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2408 a         1241 c         1604 g         2057 t
ORIGIN      
        1 ctcatatttt acgtttggtt gagcttatta ccgcagatgc aagcttttga acaccgtttt
       61 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
      121 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      181 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      241 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      301 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      361 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      421 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      481 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      541 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      601 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      661 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      721 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      781 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      841 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      901 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      961 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
     1021 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1081 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1141 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1201 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1261 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1321 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1381 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1441 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1501 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1561 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1621 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1681 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1741 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1801 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1861 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1921 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1981 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     2041 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2101 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2161 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2221 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2281 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2341 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2401 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2461 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2521 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2581 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2641 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2701 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2761 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2821 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2881 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2941 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     3001 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3061 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3121 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3181 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3241 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3301 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3361 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3421 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3481 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3541 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3601 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3661 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3721 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3781 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3841 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3901 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3961 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     4021 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4081 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4141 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4201 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4261 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4321 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4381 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4441 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4501 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4561 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4621 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4681 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4741 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4801 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4861 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4921 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4981 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     5041 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5101 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5161 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5221 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5281 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5341 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5401 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5461 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5521 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5581 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5641 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5701 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5761 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5821 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5881 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5941 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     6001 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6061 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6121 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6181 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6241 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6301 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6361 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6421 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6481 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6541 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6601 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6661 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6721 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6781 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6841 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6901 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6961 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     7021 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7081 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7141 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7201 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7261 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt
//
LOCUS       DAZUMM010000106         7158 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000106 DAZUMM010000000
VERSION     DAZUMM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.109"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..1316
                     /locus_tag="ACG8E6_002413"
                     /pseudo
     CDS             24..1316
                     /locus_tag="ACG8E6_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1835..2569
                     /locus_tag="ACG8E6_002414"
     CDS             1835..2569
                     /locus_tag="ACG8E6_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5062790.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            2585..3754
                     /locus_tag="ACG8E6_002415"
     CDS             2585..3754
                     /locus_tag="ACG8E6_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5062791.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            3770..4765
                     /gene="lacD"
                     /locus_tag="ACG8E6_002416"
     CDS             3770..4765
                     /gene="lacD"
                     /locus_tag="ACG8E6_002416"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS5062792.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            4844..5770
                     /locus_tag="ACG8E6_002417"
     CDS             4844..5770
                     /locus_tag="ACG8E6_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFS5062793.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            5789..7039
                     /locus_tag="ACG8E6_002418"
     CDS             5789..7039
                     /locus_tag="ACG8E6_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5062794.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDANRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2348 a         1194 c         1470 g         2146 t
ORIGIN      
        1 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gccgaaaagt
       61 tagctgaatc cattctgaat gcctatcaac ctgaatctgt cgatgacatg caagatgctt
      121 tgaaagatgt gtttgggccc ctttttgaaa aaatgcttca aggagaattg aataatcatt
      181 taggttatga tgcccattct aaagagccta aggaacacga taaccgtcga aatggctatg
      241 gaactaaaac gcttaaaacc agttttggtg aagtagctat tgatgttcct agagaccggg
      301 aagcttcctt tgaaccagag ttaattccta agagaaaacg agatgtctcc gacatcgaag
      361 ggaaggttct ttccatgtat gcacgaggaa tgagtcaatg agatattgcc gcaaccgtcg
      421 aagctattta tggctttgat atttcccatg aaatgatttc agatatcact gacgctgtcc
      481 ttcctgaatt ggaagaatgg caagcccgcc ccctagcaaa gtgttatgcc tttctattcg
      541 ttgattgtat gtatgttact ttacgagaaa attatgaagc caaagaatat gctgtacaca
      601 ccattcttgg ctatgatctc aaaggaaata aggagatttt aggattatgg ttaaatcaaa
      661 cagaatctaa aaatcgctgg atgcaagtat ttgatgaact gaaagcacgt ggtgtcgaag
      721 atgtcttttt catttcgatg gacggtgttt ctggcctcga agaaggcgca aaagcgatct
      781 ttccatcggt aattgttcag cgctgtattg ttcatcttgt tcgaaatgca ttgcgctata
      841 ttccaagtaa ggactataag gaagtctgcc gagatatgaa aaagttctac ggtgcttctt
      901 ctctaaacgc tgcacatgct gcttttgaca gttttcaaaa tcggtggtct cattattctg
      961 gtgctgtaga tgtctggaaa cggaactttg cacatgttga acaactattt gattatggta
     1021 gtgccattcg aaaaattatg tacactacca atgcggttga aagtgtccac tccagcttca
     1081 gaaaagtcac aaaaaaagga gcgttctcta atgagaatgc actcctaaaa ctactttatt
     1141 tacgcacgaa agaattacac gcgaagtggt caggtggtcg aattcaaaac tgggctatgg
     1201 tgcttaatca gttaatgatc aacgagacct tctcctctag aatgaaaagt atgcgattta
     1261 ccttccgtaa caacggtagg tttattcaac tatgtgtccg atcaaaattt atctgatttc
     1321 aaaatagccg agagaattat ggtggttaaa aactcttaac tatttaactt ccacagttta
     1381 gttgacaaac ccacttaatt ctgataacga aaaacatatt cgaatgatta aacagaagta
     1441 aatacttatt taaataactt ttaattttca tgttcataaa tgatttgaaa ccaaagcaaa
     1501 tgcgttgcac ttttcctccg tcttccgaaa atcgttcaat tcactggtaa gtagtcattt
     1561 gatagtttga ggcaaattgt tgattgatga agggtaggag ctaaaaaaag ataccagttg
     1621 aagagagaaa aagaaaaaaa tagagggtgt tcttgaatga aaaaagttgg aaaattattt
     1681 ttcatttcct tttattccta ttaaaatcag tgatgtcaat catattaaca agactaaaaa
     1741 tgaaaataaa aaaaagattg ttcttagtat tgactcataa aaaataccac tactatactt
     1801 acattgtggt tatagccagt taggagagac atcgatgcaa aatacctttc aatcaaaatc
     1861 actttattat cagctggtag aaatattgaa acaacgaatc gaaaacagta tggtcgctca
     1921 tgacaagctg ccatcagaaa gagagttgac cagtttttat ggcgttagtc gcacgacggt
     1981 gcgactagca ttgcaagaac tggaaaaaat gggctacatt tatcgacagc atggaaaagg
     2041 aacctttgtt tcggaattaa ggacaaatgc gactgatcta gctggcgcat atagttttac
     2101 cgaacaaatg cgggcaatgg gcaaacatcc tgaaaccaag atattggcct tcaagaagat
     2161 tgaagcaact aaatatttag cgagtcatct gaatgttcaa ctaggtgagc ctttatttca
     2221 attagaccga ttgcgcttag ctgatgattt gccaatgatg atcgagaaat cctatttgcc
     2281 agtgaagcta tttatgtccc tttctcaaga aattctattt ctgaaacctt tatataattt
     2341 gttttcagag aatttcaatc aaatcgtacg gttggctgac gaggaatttt atgccagcat
     2401 tgtccagaaa gaagatagtg agttgctaaa tattccagaa ggatcaccag tgctccattt
     2461 agttcgctca acctataatc tcgagaacga agtgattgaa tttacattaa gtgtagcgcg
     2521 ggctgataaa tttcattata agatcaggca tctgcgttcc actaactgaa ttggaggaaa
     2581 gaaaatgttt atgttctcaa aagaagaact acgaaagcta ggagcagaaa atactacccg
     2641 agaaattcga caacaaccgg aactgtggca tgagacgttt gaccgttatc aagaaacagt
     2701 tgcagattta gatcgtttgt ttacggatgc gaaacaacat gctggagagc gaccaatccg
     2761 agtcattttt acaggggccg gaacctcgca atatgtggga gatacactaa ttccgtttct
     2821 tactagtcaa ggaaatacga accagtttat ttttcaaagt attggttcga ccgatatcgt
     2881 tgctacgcca aaagaatacc tgattcctga tgagacaacg atcttggtct catttgcccg
     2941 tagtggaaat agtccagaaa gtctagccgc agttaagatg gccaatcagc tggtcaacaa
     3001 gatctatcat atcgtgatca cctgtgcacc agacggatta ttggctacac aaatgcctac
     3061 aaaaaaagaa aatctagttt tactaatgcc aaacaaagcc aatgacgctg gttttgctat
     3121 gacaagcagt ttcagctgta tgacgctgac tgcgctactt atttttgatc aagcaagcaa
     3181 tcagcaaaaa gaaagatatg ttcatcaatt aagccgacta ggagaagaag tcatccaacg
     3241 agaatcagag atccaagggt atctggcatc agactttcat cgcatcattt acttaggttc
     3301 gggaagttta ggcggattga ctagagaagc gcaattaaaa gttctcgagt taactgctgg
     3361 aaaaatcgca accgtatttg atacatcttt aggattcaga catggtccca aatcttttgt
     3421 caatgagcaa acattggttt tcctgtttgt cagcaatgac tcatatacgc agaaatacga
     3481 tgtagacatg ctggaagaaa tcaaaaatga cggtattgcc caacgcgttg ttggaattgg
     3541 tcaaaagggt tcattgttta gtggtgattc ctttgaatta gttgcagatt cgttactgcc
     3601 ggatggctat cttgctttag cagatatcat ggtagcccaa acgatcgcat tactttgttc
     3661 ggtgaaggtt ggtaatacac cagatacacc atcgccaagt ggaacagtca atcgtgtggt
     3721 caaaggcgta acgatttatc catatgaaaa ataggaaaga gtgtgaagca tgttagaatg
     3781 gtcggaaaat aaaagacaag caatggaacg tctatcgacg ccagaaggca ttattaccgc
     3841 tttggcgatc gatcagcgag gcgcgctgaa gaaaatgatg tcggctttag ggatggaagc
     3901 gacaaaggaa aaaattgagc agtttaaaac actaatttcc caagaattga ctccttatgc
     3961 ctcagcaatt ttattggatc ctgaatacgg actgcctgct gcacagaagc gtgcagcagc
     4021 gagcggtctg ctgttggcat atgaaaaaac tggttatgat caaaccgtac ccggccgctt
     4081 gccggatctg ctgtcggact gttcagttct gcgattgaaa gagttaggtg cggatgcggt
     4141 gaaattttta ctgtattacg attatgaaga agcagaggaa atcaatcggc aaaagcaagt
     4201 ttttgttgaa cgaattggaa gcgaatgcat ggctgaagga ctgccatttt atttagagtt
     4261 attgtcatat gatgctaaaa atgtagatac gaaatcgctt tcctatgcca aagcaaaacc
     4321 gcgcaaagtg atcgaaatga tgaaagaatt ctcgaaaacc cagtataaag tagatgtctt
     4381 aaaggttgaa gtaccagtaa acatgtcatt tgttgaagga aatgcaacag gggagatcgc
     4441 ctatacacgt caagaagctg ccgcttattt taaagaacaa agcgatgcaa cagatttgcc
     4501 gtatatcttc ttaagtgctg gggtatcagc ggcattattc atggagacat tgcgttttgc
     4561 caaacatgct ggggcacgat tcaacggtgt actctgtggc agagcaactt ggaaagatgc
     4621 agtcgctcct tttgttgaaa aaggagaagc ggcaacttta gaatggctga cagaacaagg
     4681 gacacaaaac attcgtcaat tgaatgaagt gataagagaa actgccatcc catggtatga
     4741 gaaagtaagt gaaagcactt gttaatgttt ctaatatatt agtagtgata agagatatgt
     4801 gtgattataa tttatcatta ttgaaactgg gaggatattt tatatgaata aaagattgtt
     4861 agattattca gcttcagatt ttttgaaagc taagccgatg gaattaaagc aggcaatttt
     4921 ggcttctgaa ggtagaacaa ttatggcaga aaatgtgcca agtagctgtc cttacatggg
     4981 aaaatcagtg acaaatgcag aattggagag agctgctgga gctgatttaa ttttgtttaa
     5041 tgcactagat ctttttaatc ctcaaatttc aggtgtacct ttaactctaa aagagaatcc
     5101 aataatctgg attaaaaaag ctttaggaag agctattggt gtaaatttag aacctgtaga
     5161 caaagaagtt ttgatgagtg aaaatcgaac tgaaatttca agtggtagaa tcgtttctcc
     5221 tgagacttta aaaaaggcaa atgaattagg ctttaatttt atttgtttaa ctggaaatcc
     5281 aggaacagga gtatctaata gaactattga gaaagcaata acattatcca aatcaatttt
     5341 taatggatta attatcgctg gaaaaatgca tggtgctggt gttgatgaac caattatgaa
     5401 cttagatata gcaaaaagat ttattaaaac aggtgtagat attttattag ttcctgctcc
     5461 atacactgtt ccgtatttca tggaaagtga tttgcgagga atcgttgatt atataaaaga
     5521 ttttaataga aataaatcta ttgaggaaaa agttctagta ctaacggcta atggaacaag
     5581 ccaagactct tctgaccaag aaactattaa aaaaattgca ttagcatcaa aagcatgtgg
     5641 agctgatatt caacacatag gggattcttt aaatggtatt tgtcttccag aaaatatttt
     5701 tgctttgggt caagcaattc ggggatatcg tcatcaaatt attatgttag gaaaatctaa
     5761 ttatcgttaa ttaaggagga aatgaaagat gatcgacaaa gttcaaaaaa tagctgcaaa
     5821 aattagtaat caaagacatc tactagcttt aagagacagc atggctgcag ttatgccatt
     5881 aatcattatt ggttcctttt ttatgttatt agcaaatttc ccgatagaag gatttacaaa
     5941 atggttatct gaaattggag tacaatcctt tttgaacaaa gcatcagatt caacatttgg
     6001 tattattgga ttagctgtta cctactcttt gggacacaat ctagcaaaga cttatggtgt
     6061 agactcatta tcagcaggat tattatcttt gagtggatat gttttactta cacctttatt
     6121 ggatgcagat ggttctatag gatttccaac aaaatacttt ggtacctcgg gtctttttgt
     6181 tggtattatt gtagcacttg tatctacaga aattttccgg tggtttgtac ttaaaaatat
     6241 tacaattaaa atgccggcaa gcgttccacc aaatgtaagc cgagctttcg ttgctattat
     6301 tcctggcttt tttgtcgttc tactatggtt tattgttgtt gtaatttgtt ataaacttgg
     6361 aattgaaaat gttcatgctc ttattgcaga tacactcgca aaaccactat ctttactgac
     6421 gaaaacttta ccaggaatta tattggttat acttattcaa tgtttctttt ggatgtttgg
     6481 tattcatggt gcacaggtaa cggggccaat aattgaaccg ttattattgc aaaactctga
     6541 tgcaaataga attgcttatc aagctggaaa agaacttcct aacattataa cttatgaatt
     6601 cttatataac tttgtttttt ctggtggtgc aggttgttta tttgcacttg ccattttaac
     6661 atttttcttt tcaaagagtc aggaaaataa aactttaggt aagttatcaa tcgctccagt
     6721 aagttttcaa gtagcagagc cccttctatt tgggtttcca actatattaa atataaaaat
     6781 gttaattcca tttgtaactg caccagtagt aacaacatta attacttatt tttcaatgag
     6841 tatgggatta gttgcaaaac ctgtaggagc aacaatccca tggacaactc caccgataat
     6901 tgccggattt ttagcttctg gtggacgaat aagtggtgca gtgattcaag taataacaat
     6961 tgcaataaat gttttgatat actatccatt ctttaaatta gatgataatg ccaagctaaa
     7021 aagcgaaaaa aatgattaga actacctata tgtttctaac tgtagttaag tatgaaaaga
     7081 ctatttttaa atttagcatt tttgcgctat cggcgtagat gaagtatcac gataacgaaa
     7141 ttagtttctt tcatttag
//
LOCUS       DAZUMM010000107         6878 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000107 DAZUMM010000000
VERSION     DAZUMM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.110"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..582
                     /locus_tag="ACG8E6_002419"
     CDS             <1..582
                     /locus_tag="ACG8E6_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS5062795.1"
                     /translation="YGTNQYSVDNSKFELCDTSMKTRDKNKFADSSKNELELYKSYKD
                     SKDIKESHNDDLLESLSTKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYR
                     QFDNWYKKFKYAIRSAEKEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTD
                     HRIKQKDDYIFASLKNKALELIELQISEGIDKQ"
     gene            658..876
                     /locus_tag="ACG8E6_002420"
     CDS             658..876
                     /locus_tag="ACG8E6_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062796.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            863..3541
                     /locus_tag="ACG8E6_002421"
     CDS             863..3541
                     /locus_tag="ACG8E6_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFS5062797.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            3532..3729
                     /locus_tag="ACG8E6_002422"
     CDS             3532..3729
                     /locus_tag="ACG8E6_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062798.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            3733..4344
                     /locus_tag="ACG8E6_002423"
     CDS             3733..4344
                     /locus_tag="ACG8E6_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFS5062799.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4329..5720
                     /locus_tag="ACG8E6_002424"
     CDS             4329..5720
                     /locus_tag="ACG8E6_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS5062800.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            5936..6250
                     /locus_tag="ACG8E6_002425"
     CDS             5936..6250
                     /locus_tag="ACG8E6_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062801.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            6243..6737
                     /locus_tag="ACG8E6_002426"
     CDS             6243..6737
                     /locus_tag="ACG8E6_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062802.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
BASE COUNT         2644 a          941 c         1334 g         1959 t
ORIGIN      
        1 tatggaacaa atcaatattc tgttgataat tcaaaatttg aactatgcga cacatcaatg
       61 aaaacaagag acaaaaacaa atttgctgat agttcaaaaa atgaactaga attatataag
      121 tcttataaag atagtaaaga tattaaagaa tcacataatg atgatcttct tgaatcactt
      181 tcaactaaaa ataataaatt agatgaagaa ttgatcgaaa catttattga agaaaatctt
      241 cttaaacaaa tatggggaga aagtattgtg aattgcttaa aactggctag caatagtgat
      301 tatcgtcaat tcgacaactg gtacaaaaaa ttcaaatatg cgattagatc tgctgaaaaa
      361 gaacaaaaaa ttcaactgaa aattatctat gaaatttgta ataacaagta ttttgtagat
      421 catgttagag aacagctatc aatgacttta cgtgacttga tacgaagagc aaaaacagat
      481 catcgtatca aacaaaaaga cgattatatc tttgcttctc taaaaaataa agcgttagaa
      541 ctaattgaat tgcaaatcag cgaaggaata gataagcaat aagaatagaa ttgagtcaaa
      601 tgaaatggag gggtatctcc acttcatttg agaactttga atataggagg ggttattttg
      661 attaaaaatc gtttaataga tcaagtttgg tgtctagatc aaaatacaga tagtctcgaa
      721 ttaatcgaga ctatttgctt caacactata gttctcgatt taagagctga aaatgataat
      781 tgtgtaactc atgtcataat taatcaaaaa atggctcaga agctctctga gagcctcaga
      841 aaatgggcta agggaggtaa cgatgaaaat aattgaaaaa aagattgcca ataaaaaaat
      901 aaattggttg gttgttaatt ttcttgttct gatagctatg attattttta caagtaatat
      961 tgctcatgcg agtattttca attggtttga tgacacaaaa agtcaaacag agtttttgaa
     1021 taattctctt tatagacctt acctaaaaaa gtacaatgga gatttcctta gtcagttcgg
     1081 gatttggctc ggatgggctg tggtaaaagg catgtttact gtgacagatt cgattcaaaa
     1141 tatgattcca gatgtgctgg atttgttcaa tttcattgaa agtacagggc taaataatgt
     1201 ttatcaatca gccgtaaata caattgtcat tggattaatg atcctttcat tgatgtttgt
     1261 tggttacaaa atgattactg gaaaaggaac agtggattta aaatcggttg gaatgaacgt
     1321 agtgatgtct atagcattaa tacttttaat gccaaccatg atttctagtg gtattcaatt
     1381 tgctaaaaca ttttataagg actctactac gattacaaat tcagatgacg gagtcgcttg
     1441 gtcattgata aaacaaggag taacagattt agcatatatt aataaaacag atcagtactc
     1501 aagcattgat aaaacggagg atcgaaataa tttaacacga aaaaattttc aacagacgga
     1561 tctcacacaa gtgttaacgg ataaagtgat tgataaattg gaaaaagaaa atccaattgc
     1621 ggacaatctt cgttatgaat tagttgaaaa ttctaataat gaatttgttg ctacaaagtt
     1681 ttctgacaac ttcttatcaa cattttctga tagcttgaaa tcaggctatt accgatacca
     1741 agcaaatcta tgggggattt caattgggtt gacagcgtta gcaatcgcct atgtgttcag
     1801 cgcgtttgtg atcattacag caatactaga acttgcattt aaacgtgtgt taggtgttct
     1861 tgtttttgca acagatattg agacaggaca gcgttcaaaa gttgttctat cagatattct
     1921 acaatgttac ttaactgttg ggtttcaagg atttgggtta tcaatgtttg ctatgtttat
     1981 caattttctg aactcaggac aaggtatctc aacaaatatt tttataaaaa caattgctta
     2041 tatctgtgcc gtttttgttt taatcaaagg cagtggaaca gtcatgagat actttggtgt
     2101 tgatattgga ctcaaagaag gctatgggca acttgcttct gcttttggaa tgggcgcaat
     2161 gctgtttaga aaaggatcaa atggatttaa tcgtgctaaa ggaagcggag aaggagaaga
     2221 tcgaaaacca gaaaagaatt tcggcgaaac tctatcaaaa aaagctggca aaactggaag
     2281 agctttggga tatgcccatg aacgtggatt atccggttta gcttcagatg gagctacaat
     2341 ggctagtgaa agagcaacaa aaccatttaa gagtatgcgt gatatagcta atgataccaa
     2401 gaataagttt aaagaaggtt tagatgatgg tacagtgtcc gctattaata aaaatagcaa
     2461 acctatgtta gctaaaaata aagaagatga aactggaaaa tatgctgatt ccgtgccaac
     2521 acgtctttca gatagaaaag atggtgctaa agttgaaaat gccgatagaa ttatgtctag
     2581 ttctgaacgt atgcgtgaag caatgaaaaa taatgctgaa tcgaataata atcctgcaag
     2641 cgcaattcag caaaaagtcc aacaagatat tgaagaacgt aaaaatgcta tgcacggtca
     2701 agctaagtct gctgaagaat taattaatca gaagcgtcat gaagcgaaat acacgcctga
     2761 agcaatgaat cgcgaggaaa taatgttaag gaaacgtgta gaagggcgga caggcgctaa
     2821 tattgacgga aaagaagcgt tgaccaaaga aagaattcaa gaagctaaaa attcaaatac
     2881 aggattagaa aaattagtca aagaaaatcc acaaaattct acaagtagtc atggtggccg
     2941 atcagtagat gtccgagaaa atattcaagg ttcactgaat ggtcatgttg gacggaatgt
     3001 tgatgtaaga gagaatttac aaagttcttc aaatgctcaa ggtactaaga caattgatgt
     3061 tcgagaaaat ctgcaaagta gcagtacaag tcaaccaaaa acagtagatg ttcgagaaaa
     3121 tgtacaaaat tctacaagta gtcaagggtt taaatcagtg gatgtccgag agaatgtgca
     3181 aggtagcagt gcaagtcaac caaaaacagt agatgtccga gaaaatgtac aaagttctac
     3241 aagtagtcaa ggattcaaaa cagtagatgt tcgggagaac attcaaaaag atagtggaga
     3301 aatgaaagaa aaaacacaaa aaatcaatat cgttgaagac agaaagtcag cgaaaaaatt
     3361 tgattcaaat catgaaacag tgattattga tagtgaaatc cgtgaaaatg ataagggatc
     3421 aaaatcaaga cgaagattca cttatgaaga caatgagtta ttcagagata cacttaatga
     3481 tcctaatcca cttttcgata atttattaag aaataaaagg gggggattgt catgcaatta
     3541 attctaaaga ttagtacgca tgatttctta gaacttgacg aattatgtga atcagtaaag
     3601 aacgcaacaa atgaaaaaat cacggtcact aattatattg aactgatgat tcaagaagaa
     3661 ttaaagatga aaaatgaaat agagagcaat ttcaaatgtt gctctctaga aaaaaatgtg
     3721 aggggataat caatggatga taaacaaaaa atgtacgaaa aaaatcgcaa acatagtgag
     3781 gccttaaatt ttcgtgtcga taaaaagact tatcttgaac tgacattgat tgcaaaacaa
     3841 aggggaattt ctagagcaga cgtttgccgt gagcggttaa agggactcaa atatgaaccg
     3901 ccgttgttag ctgaagatga tagggtagtt ttaaccaaac agttaaatca aattggtaac
     3961 aatttgaatc aagcggtaaa ggcattaaat atcattgcta aatattttag agaagaatct
     4021 gaagataaaa aagaaaagag cctacatttt agttttgatg ctagccgtta caacattttt
     4081 aaagatgaac ttgcgaatag catagcaaaa aagttgatga ttagttatcc agatgacatt
     4141 tttttggagt ccaatcggag tgccccctct ttttttaaat tatcagaagt agaaaaagaa
     4201 tatttcgaat attggataga aaagcaaact agaccgggta cgaaagaaga gtattcccaa
     4261 aaagttgatc ggaatttaat tcactcaatt cagattattg caagtttaaa agatgaggtg
     4321 aaacaacttt gggagctact ataacatcga aaggacgcaa gaaaaattta tcctcgctat
     4381 tacaatatgc agctaatcct tcaaaaaaag aaaaatgtgt tgcagtctca ggaataaatt
     4441 gtagtgatga tagtgaggaa gcactgcaag aaatgaattt agtacgtacc aagatgaata
     4501 aatctaaaga tatagcacaa gcgtttcatg ttattcattc gtttgataag agtacttcta
     4561 aagagttaag tattgataaa atgcacgaaa tagctgtcga atttgcagag aaagcttttc
     4621 caaattcgca aatcattgtt gcaagtcaca atgataaaga tcattttcat agtcatcttg
     4681 tcattaataa tattaacatg gaaacatgta aaaggttgcg tattgaccct aaagatttag
     4741 aactaatgcg aaaaattaac gatcaagtcc tcaatgaaca tggtcttgaa tcagtgaata
     4801 atgagtacta tgaaaactta gaaaagagaa ccaatcttta ttcaaacgat ccttctaaga
     4861 tgaaatatgg aaaaattaat tatcaagagt ttgtgcaaaa tgcgatcgaa gcaacgttgg
     4921 aaaaatgcga agtgaattct tttgaagatt tttcggatca tttggcaaaa gaatataaca
     4981 ttgaagttta tcaattttca aaaacaaata acaaacttgg ctatgttctt tacaaacaag
     5041 gtgtaaattt ctatgagcgt agaggtgagt taattacttt aggaagagat aaacaatcta
     5101 gaaaagaaaa aagtcagtat gtcgaacgta cttttagtgc aagaaagtta gggaaaagat
     5161 attcatttga atctgtttct gaacaacttg aaaagaattt agaagaatca catataaaaa
     5221 atttttttaa tatggaaaga gagcaagtta tgtcgctaat tagtggagta aattacaaaa
     5281 ctggtgagtt aaatgtaccg tttgtggaat tagattccat cgcacaaaag caatttagcg
     5341 accgtgaagg cgatgaaaaa ttcattaaat atgatgactt cttgcgagag gtagacaagc
     5401 attttgtaaa tacaatgtct ttagaagatg atcaagaata cacgattaaa cttcatacgg
     5461 atgtgaatcc tcaaacttta cgtagtgcga aaagtaaaac aatttctatt aaaaagactg
     5521 atcagaaaga gttatttcta ccggcaaagg gttttgaaaa ttgttctcat acaccttctg
     5581 ctaaacagat tcactatcaa atcagtcgca taatgaatga cgatgtaaaa atgtatcgtg
     5641 ctaaaaagga ttatgaattc acacaacaaa tgattgaaaa tcagcaaaaa aggcaacaaa
     5701 attttgaaat ggaattctga atgggcttgt tagtggactt tccagttttt tgcctatgaa
     5761 gagttgcttg gaaagaagca gtttttagga catacgccag ctacccatct acttgccagt
     5821 caatccgccc cgctcacgct aggctacttt ctggcaagtc agatggcggg taaatctctg
     5881 agggagcaag ctccctacag actccctccc aaaaaaagaa aggtggaaga aaaaaatgaa
     5941 ctatccatat tttaaagtct cggcttcaga agaaactaaa gaaatattca acaattttta
     6001 caaccaaaac aaaggaattt ttggttcaaa ggcaaatatg tttcgtgtta tggtttcaaa
     6061 tttaccagtg ttagctagtc catcaaataa taaatttaat gatcctgaaa gcattaaatt
     6121 tgaacaaaaa atttcagaac tagaatcaat gataagtaat gaagtaattg aaaagctgga
     6181 tgacattgat caaaagttat cgtattctct aaaaaacaaa tacaaaacgg aggagaaaaa
     6241 agatgtttaa agtttacttc ccgtttcagt ggctcgttaa aggaattctt ttagtgctac
     6301 taggattgat tctttttgga agttatctaa caattaaagc cagacatttt ccagaagaaa
     6361 agccaataaa aattgtaaca aaagatttaa aaataggtga aacttatcag gcagatgggg
     6421 ttgaactaac atttaaaaat attgaagtga ccgaaaaaag aaatgaattt gcacaacatg
     6481 atcgtgtatt acaagtgacc tatacactta taaatgatac agaaaatccg atacgtccac
     6541 aacaatggtc gattgaaaat aaggatggtg aagaacttta tgagtatgat atgatattgg
     6601 ataatccaga gtatattgaa agtggcaaag aagaaacagt aaattactat tatgaattac
     6661 cgattgaaag gcaggactac caaatcgtag tacgtagaga gaatggttac atagttgggt
     6721 tgccatttac tttataagtt ttattttgtt agtagttttt aaggtaatat cagttttgtg
     6781 atttttgaaa atggaactgg gagaatggct gaggaaaata aagttgttcc gtttgaaaca
     6841 ttagtgataa ataaggaaga gtttgagcaa ctttttct
//
LOCUS       DAZUMM010000108         6671 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000108 DAZUMM010000000
VERSION     DAZUMM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.112"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..164
                     /locus_tag="ACG8E6_002427"
     tRNA            75..164
                     /locus_tag="ACG8E6_002427"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:109..111,aa:Ser,seq:gga)
     gene            179..250
                     /locus_tag="ACG8E6_002428"
     tRNA            179..250
                     /locus_tag="ACG8E6_002428"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:212..214,aa:Glu,seq:ttc)
     gene            266..338
                     /locus_tag="ACG8E6_002429"
     tRNA            266..338
                     /locus_tag="ACG8E6_002429"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:299..301,aa:Val,seq:tac)
     gene            348..420
                     /locus_tag="ACG8E6_002430"
     tRNA            348..420
                     /locus_tag="ACG8E6_002430"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:381..383,aa:Asp,seq:gtc)
     gene            456..531
                     /locus_tag="ACG8E6_002431"
     tRNA            456..531
                     /locus_tag="ACG8E6_002431"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:489..491,aa:Phe,seq:gaa)
     gene            533..613
                     /locus_tag="ACG8E6_002432"
     tRNA            533..613
                     /locus_tag="ACG8E6_002432"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:567..569,aa:Tyr,seq:gta)
     gene            620..693
                     /locus_tag="ACG8E6_002433"
     tRNA            620..693
                     /locus_tag="ACG8E6_002433"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Trp,seq:cca)
     gene            696..768
                     /locus_tag="ACG8E6_002434"
     tRNA            696..768
                     /locus_tag="ACG8E6_002434"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:729..731,aa:His,seq:gtg)
     gene            780..851
                     /locus_tag="ACG8E6_002435"
     tRNA            780..851
                     /locus_tag="ACG8E6_002435"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:812..814,aa:Gln,seq:ttg)
     gene            897..967
                     /locus_tag="ACG8E6_002436"
     tRNA            897..967
                     /locus_tag="ACG8E6_002436"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:929..931,aa:Cys,seq:gca)
     gene            988..1072
                     /locus_tag="ACG8E6_002437"
     tRNA            988..1072
                     /locus_tag="ACG8E6_002437"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1022..1024,aa:Leu,seq:caa)
     gene            1134..1206
                     /locus_tag="ACG8E6_002438"
     tRNA            1134..1206
                     /locus_tag="ACG8E6_002438"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1167..1169,aa:His,seq:gtg)
     gene            1216..1287
                     /locus_tag="ACG8E6_002439"
     tRNA            1216..1287
                     /locus_tag="ACG8E6_002439"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1248..1250,aa:Gln,seq:ttg)
     gene            1305..1389
                     /locus_tag="ACG8E6_002440"
     tRNA            1305..1389
                     /locus_tag="ACG8E6_002440"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1339..1341,aa:Leu,seq:caa)
     gene            1644..2519
                     /locus_tag="ACG8E6_002441"
     CDS             1644..2519
                     /locus_tag="ACG8E6_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5062803.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            2623..3387
                     /locus_tag="ACG8E6_002442"
     CDS             2623..3387
                     /locus_tag="ACG8E6_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFS5062804.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            3374..4816
                     /locus_tag="ACG8E6_002443"
     CDS             3374..4816
                     /locus_tag="ACG8E6_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5062805.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            4827..6158
                     /locus_tag="ACG8E6_002444"
     CDS             4827..6158
                     /locus_tag="ACG8E6_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFS5062806.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            6171..6629
                     /locus_tag="ACG8E6_002445"
     CDS             6171..6629
                     /locus_tag="ACG8E6_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS5062807.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT         2100 a         1091 c         1481 g         1999 t
ORIGIN      
        1 tcatgatgtc gtaggttcga gtcctactgc cggagttctc attttgagaa agagtattga
       61 gcttctttca ctctggagag ttgtccgaga ggccgaagga gcatgattgg aaatcatgta
      121 gatggtgcca aactgtctca agggttcgaa tcccttactc tccgtagcaa atttacctgg
      181 cccgttggtc aagcggttaa gacaccgccc tttcacggcg gtaacacggg ttcgaatccc
      241 gtacgggtca ttttaattga atgttggagg tttagctcag ctgggagagc atctgcctta
      301 caagcagagg gtcagcggtt cgatcccgtt aacctccata atgaactggt tccgtggtgt
      361 aggggttaac atgcctgcct gtcacgcagg agatcgcggg ttcgattccc gtcggaaccg
      421 tcgttcttgt tattcaatta ataattttaa aatatggcgc ggtagctcag ttggtagagc
      481 aacggattga agctccgtgt gtcggcagtt cgattctgtc ccgcgccacc atggaggagt
      541 agcgaagtgg ctaaacgcga cggactgtaa atccgttcct tagggttcag tggttcgaat
      601 ccactctcct ccattttata ggggcatagt ttaaaggtag aacagcggtc tccaaaaccg
      661 ttagtgtggg ttcaattcct gctgcccctg ccatggcgaa tgtggcgaag tggttaacgc
      721 accggattgt ggctccggca ttcgtgggtt cgattcccat cattcgccct ttttattctt
      781 ggggtatagc caagcggtaa ggcaagggac tttgactccc tcatgcgttg gttcgaatcc
      841 agctacccca gtttctataa tttattcttt attttataga ttgataatag tataatggcg
      901 gtatagccaa gtggtaaggc agaggtctgc aaaaccttca tcaccggttc aaatccggtt
      961 accgccttag tactaataaa tcaacttgcc ggcgtggcgg aattggcaga cgcgctggac
     1021 tcaaaatcca gtgcccgttg agggcgtgcc ggttcgaccc cggccgccgg tattgactaa
     1081 gatgtcttca tcttagtttt ttattgagaa attaaataac atcttttatg atggcgaatg
     1141 tggcgaagtg gttaacgcac cggattgtgg ctccggcatt cgtgggttcg attcccatca
     1201 ttcgcccttt attcttgggg tatagccaag cggtaaggca agggactttg actccctcat
     1261 gcgttggttc gaatccagct accccagtta ttatttttaa atttgccggc gtggcggaat
     1321 tggcagacgc gctggactca aaatccagtg tccgttgagg acgtgccggt tcgaccccgg
     1381 ccgccggtat aattatcaat atagcaactt tcttatagag agttgctata ttttttttgt
     1441 ttttatgtaa ttacctccaa atcaattcga tacaagtatt atcataaaac gagtaaaaaa
     1501 gatattgata acgcttttat tatgtgttaa actatttttg taaattatgt agggtgctac
     1561 tattcaatta ggcacgacct aagaaagggt tctttcttag gtcgtttttt gtctttaaaa
     1621 attgtttgga gacaaaaata tgtatgaagg agtctgcaag ttgttttgtt ttatcatata
     1681 gaggaggtga attaacggtg tatgtgatga aaaagctgaa tcataatgct gtattgatcg
     1741 atgacgatgg aatagaaaaa attgcgatgg ggaaagggat tggcttttct gctaaagtga
     1801 gtaagccatt tgatgttgag gatgctgaca aattatttgt attagattct aaagaaaaga
     1861 tcaaacgatt cagtgagata gtctcgcaga ttccaatcga atttatggat tttgcggaag
     1921 aagtgattga actgataaca gctaggatct cctctccatt agattcaaat atctatatta
     1981 cattgacaga tcatatttat tttgctattc aacgaaaaag agaagataga gatgtcacag
     2041 caattatgtt gccagaaatg agattccttt atccaaatga atatgatata gcagtagaag
     2101 tggtcgagcg aatcaatcat cgatatcaaa ccgagttagg gtcaaatgaa gtaggattca
     2161 tcactatgca tatcattaat gctgagctag gagagcctaa tagtttaaag agcttaaaaa
     2221 tagtagaaat gacgaccacg attttgtccc atctagaaaa atcctattca aacgtttatg
     2281 ataaagaatc actgacttat catcgcttaa tggttcatat caaattcctg gttaaacgct
     2341 tgatttatca ggaagaaacg ccagtagaaa atattggttt ttttactgaa acatttaaac
     2401 atagcaaagc gtattgtaca gcactaaaga ttaaagatat catcgaacag caatttcaac
     2461 ttgccattca agaaaatgaa atcatctatt tggcaattca tctagcaaga atctattaaa
     2521 ataagggcgt aactatctat aggcgcaacc aaatgacaca tagtaaatgg actatgatta
     2581 tttggttgcg cctattttag tagaaaaaag aaggagaatg aaatgggaaa agtgattttt
     2641 aattcggatg attttggcta tagccatggt gtgaattatg ggattatgga tgcttatcag
     2701 cgaggaattt tgacatcaac tacattgatg gcaaatatgc cgggtttcga gcatgctgtg
     2761 aaactaagaa aggaaatgcc taggctaggt gtaggagtcc atttaacatt gacgtgtggc
     2821 aaaccactcc tcaaaactgt cgatactctt atcgaaagcg gagagttcag atccttatct
     2881 tattatcagc atccatttaa cattgacaaa gatcaactgt atcaggaatg gaatgctcag
     2941 attcaaaaaa tttatcatgc ggggatcata ccaactcatt tagacagtca tcatcatact
     3001 catactttgg atgaaaatca ggaggtagtt gttacattag caaaaaaata tgatttgccg
     3061 gttcggagca actttgaacg aaaagatgaa gttcgtcatg tcgattattt tgagccttgt
     3121 ttcgatgtcg taggagaaac tgatgaaaaa aaacgacaaa tcaatacgag tgtggaagaa
     3181 tatttggcaa gtttactcat gatattgaga gaagataaaa cgatcgaagt catgtgtcac
     3241 gcagcgtatt tggatcagga attgttaaat ggttcaagtt ttgtgtatcc tcgtacccac
     3301 caagtcgaat ttttaattca ttcagcattt gctcaaaaag tgaaaaatga tcctttgatt
     3361 gagttagtaa catatgagga tatttaatag aggaggaata aaaatgaatg ttaaatcaaa
     3421 tgtgcagaag cttggaagag cgatgctact tccagttgca gcgatgccat tagcgggatt
     3481 gatcatgcgt ttatcagctg acgatatgct gaacatccca gtaattggtg cagctggaaa
     3541 tgccgttttt ggaaacttgg atatcttatt tgctatagga gttgcaatcg gttttgtcaa
     3601 aaataaggac aaaggaatac ctgcacttac tggtttttta gcgattgcta ccttgaaaaa
     3661 aggcttagaa attatggacc cagaagtgaa tatggggatt tttggtggaa ttatttccgg
     3721 tttagtagcg gcatggacgt ataatcgctt taaagaacaa aaattaccag tagttttttc
     3781 ttattttgcg ggcgaaaaat tcccattgac tatggtgatg attctacaaa caatcgcagc
     3841 tgtttttttg ggctttatct ggccgctcat ccaaattggg attgataatt ttgcaaaact
     3901 gctcgttgat atgggagcat taggtgtggg gatatttatg tttttgaatc gtttgctgat
     3961 cccctttgga ctgcaccatg tgttgaatac gtatatttat tacgatttag gaagctatac
     4021 atctcctagc aaggaagttt tcagaggaga gatgacacgc tttatcaacg gagatcctca
     4081 ggctggactg tttttatcag gtttttttgt agtaatgatg tttggtatac cagctatatg
     4141 tctagctatc tataaagcag cttataaaga aaataaagag atggtcaaag gaatcatggg
     4201 aagcggtgca ggaacttcat ttattgcaaa tatcactgag cctgttgaat ttagtttcat
     4261 gtttctttct cctatgttgt acgtgatcca tgcacttttt gccggtttag cagggattgt
     4321 ctgttactta ttcaatatac gaattggttt tacattcgga gcatgtattg ttgattatct
     4381 catcaatttt cgaatcgcta ccaatgctat tttgattctt ccaatcggaa ttttcttttt
     4441 tgccttgtat tacgttactt tttattattt gatcaataag agaaacattc agacattagg
     4501 acgtgaagct aaggcagagt ttggcaatga agtaacctta gaagaaacag agttagggtt
     4561 agcaagtaaa aattatgaat atatggcaac aaaaatgcta caagcgtttg gaggaaaagc
     4621 caatatcctg gatgtctata gttgtaatac acgcctacgg gtagaggtag tggatcccac
     4681 catggtcgag gaacagcgaa tcaagcaatt agggataagc gggatcatta aaccaactga
     4741 aaaaaattat caaattatca tcggcttaga agtgacttat gttatggcag aattcaataa
     4801 attattagaa gaatagaaag gaaaagatga tgatgaaaaa acagattatc actatagctg
     4861 gaggcgggag tacgtataca ccaggaatcg ttcaagccat tttgaacaat gaaaaacggt
     4921 tgccattatc tgaaatacgt ttatatgata ttgatgaaga aagaaatatg gatatgtact
     4981 tgattgttaa atttatgctg aaaagaaaag gtttttcaaa tattcgcatt cgtgcaacgg
     5041 atgatccaaa gcttgctttt acaggatgcg attttgtttt ctcacaaatt cgtgttggag
     5101 gattggagat gcgagaaaag gacgaaaaga ttccacttaa acatggatta gtaggacaag
     5161 aaacgtgtgg actcggagga tttgcttatg ggatgcgctc aatcaaaggt ttattggaaa
     5221 ttgtcgatca tatacaagac tatgccccca aagcatggat tttgaattat acaaatccag
     5281 agtcgatcgt ttctgaagct gttcgtcgta aatatccgaa tgtaaaaatg attaatgcct
     5341 gtgatatgac tatttcaatt gaagaaacga ttgcagtgaa ttacggttat gatcgtaaga
     5401 attggatcgt tacgtattat ggtttaaacc actttggctg gtatacatcg atttatgata
     5461 aagaattgca acgagaaatt atgccagaga ttattgaaaa actaataaca aaagaaatgc
     5521 aagtcgccga ttttaatata ggagataaaa cttggcaaaa aacctttcaa atgatgtcag
     5581 tcatcacgaa aaattttcca agtaatatcc cgaataatta cttagagtat tacttatatc
     5641 ctgatatggt cgttgagcat actgacaaag agtatacacg tgctaatatg gtcatggatg
     5701 gacgagagaa aaataccaaa gaaatggctg ataaaatacg ccgcggtatt gtggaagaag
     5761 tattgaactt taattttggg gagcatggac aatatatcgt cgatatagct atttccttgt
     5821 tgaatgatga tcgtcgtcgc tttatgctaa tcgttcctaa tcaaggggcg attccgaatc
     5881 tacggtcaga tgcagttgtt gaaataccgg catatgttgg tgcaactggt gtggagccaa
     5941 ttacactaag aaaaccaatc agtgattttc ataaaggatt gatggaggca caagttgcag
     6001 tagagaagtt actagtagat gcttactttg aaggttctta tcaaaaagct ttacaagcat
     6061 ttacattgaa tcaaaccgta ccaaatgcca gagtcgcaaa aaaaatacta gatgaaatga
     6121 tcaaagtaaa caaaaaatat tggccagcat taaagtagag gtgataaaaa gtgcatttct
     6181 ttaaaacaag aaacattcta aaagctgtag ctagtggtca aattattcca ttaacagcag
     6241 taaaagacga ggtcttttct tcaaaaatga taggagttgg atacgctata acgaatcatg
     6301 atggatatat ccatgcgcca gttaaaggaa taatcgataa tatatttcct acccgacatg
     6361 ccataacgat cgaaagtgaa tctggcagaa aaatactgat ccatatgggt ttagatacag
     6421 ttgaactcga aggaagtccg tttcagatac gggttgaaag taaccaggag gtaaaagaag
     6481 ggacactgtt agctattata gatttgaaga agttggagca agcacaaaaa gatacggtga
     6541 tcattgtagt ttctccaggc atttttgaag gaaatttatt aaaacaaaac caagaagtaa
     6601 agattaaaga tagttgtttt gaattttagt gtacgatgaa aaaagttaaa tagggtgata
     6661 tttatgggtg a
//
LOCUS       DAZUMM010000109         6623 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000109 DAZUMM010000000
VERSION     DAZUMM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.113"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACG8E6_002446"
     CDS             complement(105..629)
                     /locus_tag="ACG8E6_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFS5062808.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8E6_002447"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8E6_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFS5062809.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8E6_002448"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8E6_002448"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFS5062810.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACG8E6_002449"
     CDS             complement(2351..3031)
                     /locus_tag="ACG8E6_002449"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS5062811.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACG8E6_002450"
     CDS             3157..4026
                     /locus_tag="ACG8E6_002450"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5062812.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACG8E6_002451"
     CDS             4052..4567
                     /locus_tag="ACG8E6_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFS5062813.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACG8E6_002452"
     CDS             complement(4614..5177)
                     /locus_tag="ACG8E6_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5062814.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACG8E6_002453"
     CDS             complement(5232..5411)
                     /locus_tag="ACG8E6_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062815.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACG8E6_002454"
     CDS             complement(5488..5997)
                     /locus_tag="ACG8E6_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062816.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACG8E6_002455"
     CDS             complement(6093..6473)
                     /locus_tag="ACG8E6_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5062817.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
BASE COUNT         1861 a         1400 c         1172 g         2190 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcc
//
LOCUS       DAZUMM010000110         6531 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000110 DAZUMM010000000
VERSION     DAZUMM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.114"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..192)
                     /locus_tag="ACG8E6_002456"
     CDS             complement(<1..192)
                     /locus_tag="ACG8E6_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5062818.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDL"
     gene            315..917
                     /locus_tag="ACG8E6_002457"
     CDS             315..917
                     /locus_tag="ACG8E6_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5062819.1"
                     /translation="MEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRG
                     ERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIAS
                     LDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKA
                     LNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            972..2138
                     /locus_tag="ACG8E6_002458"
     CDS             972..2138
                     /locus_tag="ACG8E6_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5062820.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(2227..2619)
                     /gene="rpsI"
                     /locus_tag="ACG8E6_002459"
     CDS             complement(2227..2619)
                     /gene="rpsI"
                     /locus_tag="ACG8E6_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS5062821.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2635..3063)
                     /gene="rplM"
                     /locus_tag="ACG8E6_002460"
     CDS             complement(2635..3063)
                     /gene="rplM"
                     /locus_tag="ACG8E6_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFS5062822.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(3597..4064)
                     /locus_tag="ACG8E6_002461"
     CDS             complement(3597..4064)
                     /locus_tag="ACG8E6_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFS5062823.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(4184..5722)
                     /locus_tag="ACG8E6_002462"
     CDS             complement(4184..5722)
                     /locus_tag="ACG8E6_002462"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFS5062824.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5802..6488
                     /locus_tag="ACG8E6_002463"
     CDS             5802..6488
                     /locus_tag="ACG8E6_002463"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFS5062825.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
BASE COUNT         1933 a         1275 c         1198 g         2125 t
ORIGIN      
        1 caaatcatta agtaaccaat ttaccaactt ttgataagta gatgctctaa tactttcttt
       61 gctcccattt tcgattttga catatgtttt ccgatttata cctatcaatt ttgctgcatc
      121 gtttttagta agtataagat cggctctatg tcttcttaag cttctaatga attgatcatt
      181 aagagttatc attcagcgcc tcctttcaaa ttattcactt tgtggttttt atcttaataa
      241 gcacatttta aaccacaaaa cgagtaatat caattaaaat attactcaat ttgtggtttt
      301 catgctataa tagtttggaa tttactatag ggggcgttac tttgaataga ataaaatttt
      361 atattgattc cagtaaaaag accattaaac aaattagtac agaaactggt attaactata
      421 caactcttac gaactattac cgtggtgagc gaaatcctag aaatactgaa acatggaata
      481 tccttgctga ttatttccat gttcctgtaa gttatttgat gggattagac gaaacaaatt
      541 ccatagaaca acctaatctt gatagaataa aaactgctcg tttagaaaaa aaggacaaaa
      601 tagtcaagaa tattgccagt cttgactcag attattttac tgaattatct aatctatact
      661 tttccatgac ttctttactt gaagtcaaac ttcaagaaga tgactccgac tttctttttt
      721 taaaaagtat tctagaggac tcctttgaat acacgattct atttgccaaa caaaagaagt
      781 ttattaataa agctttaaac ttaaatatcg atgaaagaaa agagttagaa tctgagttat
      841 ttggcaactt aacccaagta caggttaaca atctaaaaag agagataaca gctcgattaa
      901 tatcaaaact tcaataaaac taattattca atcagccaac tcgccaagtt atcagattgg
      961 agagattagt tatgtcaata caaaagtaca caacaaaaag cggtgcacga tatagtgtcc
     1021 gcttgtatgt tcgtaaagat gaaaatggaa agcaagaata ctatgtaaaa aaagggttta
     1081 aaactgaaaa agaagccaag ttacacgaag caagaaaaaa ggtcgagatt gaagataccg
     1141 gctatcataa gccttctaat gacttgttta taacagtgta tgaagaatgg ttgccttctt
     1201 ataggaatcg tgtacaagaa acgacatatc aacgtacaaa agaccttttc cgcttgcata
     1261 tactaccaaa attcgggaat aagtccattt ctaagattac tcctgttcac tgtcagaatg
     1321 cagtcaatga atgggcttta aaattcaaga atatcaaaca gcttaagtct tacagctctc
     1381 aaatatttga gtatgccatt tttgcagagc tattacaccg aaatcctatg gcaaatacaa
     1441 atctaccaaa gcgggaaaag aaagaaaatg aaaattactt ttcattagaa gaactaaaag
     1501 ctttttggga tattctcaat aaagaagagc ctttaaaaca tattttaatc ttccagcttt
     1561 tgattactac tggtatacgt aaaggagagc tagctgctct tcattggtct gacatttatt
     1621 ttgaagaaca gctaatgtat gttagaaaga gctatgcaac tattcgaaat gaagagaaga
     1681 atgcaagaag aaaaactgtc aggattcaaa aaaatacaaa aaatatatct tccgaacgaa
     1741 tattaccaat tgatgcacaa actattgaaa tgttaaaaat ctggaaaaaa gaacaagctc
     1801 gagagctttt gcagtttggc ataaatacaa atacaaaaaa tcaacttatc tttacctatg
     1861 taaatgcaga cggtgacatt aatcaacctt tgcatgcgga ctattcaaat aatatattga
     1921 aacgtttaga gaagaaatat aaatttaagc acgttactat tcatggattc cggcacactc
     1981 acgctactct tctactagaa ggtggagcaa gtatcaaaga aacacaagat cgattgggtc
     2041 ataaaaatgc tgaaactacc cttaacactt attctcatgt tactgaaaaa gctcaacgaa
     2101 atgcgttaga aaaattcatt gcttacactg gtttataata ttgtcattac ctttctcatt
     2161 accagaacga aaaaaagaca ccttctcgca atgagaaagt gtctgaaatc gttggtataa
     2221 caacgattaa cgttttgaaa actgtgaagc tttacgtgct ttcttaagac ctggtttttt
     2281 acgttcgacc atacgtgcgt cacgagtaag taagccagca cgtttaagag ctaaacggaa
     2341 gtcaggatca acttctagca atgcacgagc gataccatga cggatagctc ctgattgtcc
     2401 agcgtatcct ccgccgttta cgttaacaaa tacgtcatat gcgccttttg tttcagtaac
     2461 agcgaaaggt tgattgatta cttcacgcaa gtcagcatgt ggaatgtatt cttcaacatc
     2521 ttttttattt acagtaattt tgccagttcc tggtactaga cgtacgcgag caactgcgtt
     2581 tttacgacgg cctgtgccga tatattgaac ttgtgccaat gaaattccct cctattagat
     2641 taagtttgtg atgtctaaaa cttctggttg ttgtgctgca tgtggatgtt ctgctccacc
     2701 gtatacgttc aattttgtaa attgtttgcg gcctaaagtg tttttaggaa gcataccttt
     2761 tacagaagtt tcgatcaaac gacgagaatt tttagcacgc aattcgccag cagtgattga
     2821 tttcaatcct cctgggtagt tgctgtgacg gtaataaatt ttgtcagtcg cttttttacc
     2881 tgttaatttc actttgtctg cattgatgac gatgacgaaa tcgccagtat ccaaatgagg
     2941 tgtgaaagtt ggtttatttt taccacgtag tacagatgca acaactgttg aaagacgtcc
     3001 caatggaaca tcagttgcgt ctactacata ccatttacgt tctacttcgc ctggtttggc
     3061 catatatgtt gtacgcacgt tattttcctc caatttaagt tctgatgttt gacatacact
     3121 tcgagtttcc ggggctcttt gtggggcaaa caataccatt taacatatta cccgctaatc
     3181 tagtcaatgt caacgttttt ctgcttgatt gaaaaattta tatgaaactt tacttctttt
     3241 ctttctttta tataaaaggg tcgtttcctg tcaaaatgaa aaacaaaaag aggctgtgac
     3301 aaaagtcttg tcacaacccc ttattttgaa taaacggtgt tctcctgcca actcctcggt
     3361 cacaagtcac aatatccata aaacataaga agctgttttt taaaattgtt tcttgtgact
     3421 cggagctgaa cggcaggctt acaacctctt ggtaaacggt gttcaatcag ctgaccctcg
     3481 gtattagctc aaaaaacggc aaaatatgag gaactatttt gacgtttgtt tctctaatac
     3541 tcaggtcaaa acgctgattt cacaacctct ttttcggtca agttcaacaa cagtttttac
     3601 gcgtcgatct ctggcgcttt gcccaaatga gcttggatca tccagatacg tttttctgtg
     3661 gctgttttga attcgataaa gatatcttgt gttggatcgt cgccttcttc tccagaaact
     3721 tcgattcctt tttggtaaag atctgctaga taacgatagc ctgcaacaag tgtttccaaa
     3781 cgttctggaa tagtacgatc ccagcggcca atttcatcag ggatttttgt gttatctgca
     3841 aattcacgca atgtagaata aggtgaaccg tctaatgtga tcaagcgttc ggatacttca
     3901 tctaactgag cgttcaaatc atccatgaac tcatccatca ttgggtgcaa tgtaaggaaa
     3961 cctggtcctc tcatgtacca atgtgtttgg tgaacaacaa cagacatttg gcttagatct
     4021 gcaactaatt gatttaatac ttctttggtt tgttggaatt tcatctttct tgcctcctaa
     4081 cattttctta cacttaaaat aatagtagac tttcaatgaa aagtacaaca aaatcattta
     4141 cctgagaacg attttcagtt cgctttttta tccgtaaagc caatcatttt tcagaatcag
     4201 ctaattcttc tcgcagttga gcaaattgct gataagtctg tgctaaatgc ttttgtgtcg
     4261 aaacaaataa tggatagatt ttctgatatt ttttatgatc agtttcttgt actccaactt
     4321 gctctttcaa ttctatcaat tcctgtgctt cactgatact ttggatcttt ccgatgcttc
     4381 tccagcctaa aatcacagct cctaatgcgg atgcttctgt gacagtaggg aaaacaagcg
     4441 ggtaacccag tacatcagcc atcatctgac gaaaaatcgg agattgagca aacccgcctg
     4501 tagcacggat ctctgtgagc tgattttcct ggcaaactcc ttttaaaatc cgataaaggt
     4561 tgagacagat cccttctaga atcgccttca tcatttcttt ttctgtatgt tgccgctgta
     4621 aaccgataaa actgccaaac gcatctgcgt cccaaagcgg tgctcgctca ccaagtaaat
     4681 agggatgaaa aatcagacca ttggcacccg ctggcacaaa agcgatagcc gaaagcagtg
     4741 catcatatgg attttctcct ttttctttag cagattgaac gacttcctgt aaaaatgtat
     4801 gccctgccca atcaaagatg ccagcaccat tgctagtcgc tcctcccact acccaatgtt
     4861 tctgatccag aacataacaa aacgtctcca tttctggatg gaactgaggc tgagtagtaa
     4921 taaaacgaag tgctccgctc gttcctacgg tcaaagcggc gatcgtatca ccaaaagcac
     4981 ctaaccctag attagaaagc ggcccgtctg cccctcccaa aacaaatgat atatcatttg
     5041 gcaaacctag ttctgctgct gtttttgatg gtagacctgt tacttgatag gtggaaggaa
     5101 agacttgcgg caattgctct attcgaatgt tcaagtagtc aagcacttct tctgcccatt
     5161 ggaattcatg gatatcaaaa tatccagttc ctgaagctgt tccgtagtcg ctgatcagtt
     5221 cgccaaacaa tctataaaag atatagtctt tgataccgac aaaataagca gtttttttga
     5281 ataattcagg ttggctgttt tgcagccact tgattttgtt caatggtgtc attggatgaa
     5341 tcggcgtccc agtctgccga taaaattctt tgccaagcac ggtatatttc agtttttctg
     5401 cttcgtccgc tgctcggtta tctgcccaag tgatcattcg agataacggt gttttctctt
     5461 catccaacaa tatcaggcca tgcatcgcag aggagaagga gacaaattga atgattgtgt
     5521 ctgaaggcaa ctgtttaatt acttctttta taacggtgca gacaccttga aagatcaatt
     5581 cctgatcttg ttctgccatc ccttgtgttt cttggatcaa agggtagcga aaataagcgg
     5641 atgctgcttc ttgggcttca tctgtgaaag caactgcttt ggtttgtgtg gttccgatat
     5701 ctaatccgat tgaaacgttc attttttcac ctcaaatttt atgtatgtac aaaaatttag
     5761 tttaagcttg cgtatcttgg ttttgaaaag ggggagatgg cgtgttactt tattttatca
     5821 ttggttgctg tatcggatca tttttatgtt taaccgccca gcggattccg ttaggtcact
     5881 ctattattta tcctagatct cattgtgtca aatgctgtcg ttcgctttct tggtatgaat
     5941 tgattccgat tctctcgatc atcgtccaac gatttcgctg ccgctattgc aggtgtcggt
     6001 tgcccttcta ctatttgttg gcagaagcct tatgtggtgg tctttttgcc tggttcttta
     6061 ctttttcttc cgcaagaaat ttttcgacgt tcatatggat gctttcggct ttgctatttt
     6121 cgttgatgga tttattttac ttcatggtcc attctcacac gctattctgt tcttgggcaa
     6181 ttttatggat tttttggttg caggccggcg tcttccaatg gcaaagtgtg ctgttaacgt
     6241 ttatcgtagg cggtgtgtgc cttcgctatg gtcaatccta tctcggagct ggcgatactc
     6301 tgttgatgct ttcttggagt ggtggcttgt ctctagaaga gttgcttcag atcttgtttc
     6361 ttgctagtct gtttggaata tccgtttttt ctgtttactt tatctgtcat aaaaaaaagt
     6421 tggaaaagct gcctttcatt ccttttttga gcagtgcgtt gctgattgtc cttcatctta
     6481 ggtattgaaa aaaggttgtg acaaacgact tgtcacaacc tctgatatat a
//
LOCUS       DAZUMM010000111         6170 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000111 DAZUMM010000000
VERSION     DAZUMM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.115"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACG8E6_002464"
     CDS             725..811
                     /locus_tag="ACG8E6_002464"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS5062826.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1618)
                     /locus_tag="ACG8E6_002465"
     CDS             complement(932..1618)
                     /locus_tag="ACG8E6_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062827.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTC
                     YEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTE
                     EACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSV
                     KEWLDQQQQN"
     gene            complement(1625..1903)
                     /locus_tag="ACG8E6_002466"
     CDS             complement(1625..1903)
                     /locus_tag="ACG8E6_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062828.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1863..2777)
                     /locus_tag="ACG8E6_002467"
     CDS             complement(1863..2777)
                     /locus_tag="ACG8E6_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS5062829.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQVFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2759..3139)
                     /locus_tag="ACG8E6_002468"
     CDS             complement(2759..3139)
                     /locus_tag="ACG8E6_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS5062830.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3589..3876)
                     /locus_tag="ACG8E6_002469"
     CDS             complement(3589..3876)
                     /locus_tag="ACG8E6_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS5062831.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3896..4120)
                     /locus_tag="ACG8E6_002470"
     CDS             complement(3896..4120)
                     /locus_tag="ACG8E6_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS5062832.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4575..4778)
                     /locus_tag="ACG8E6_002471"
     CDS             complement(4575..4778)
                     /locus_tag="ACG8E6_002471"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062833.1"
                     /translation="MAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKGNKKG
                     ELKLNPNYYNSQNEEVFVEKLDK"
     gene            complement(4760..5293)
                     /locus_tag="ACG8E6_002472"
     CDS             complement(4760..5293)
                     /locus_tag="ACG8E6_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS5062834.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5314..>6170)
                     /locus_tag="ACG8E6_002473"
     CDS             complement(5314..>6170)
                     /locus_tag="ACG8E6_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS5062835.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1632 a         1152 c         1041 g         2345 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tacccctggc aacaaaaacc
     1501 tcttaaatcg aattttagag ccttttaatg aatatcttgt ttgataagcc ttatagctaa
     1561 gccaaataat tcctaaaata ctggcaaaaa tgagtagata gggtaaatag aatgccattt
     1621 ctatttattc tccccatacc agccaaaagg ctaacagtgt catgattaag caacataagg
     1681 gggcaagaat atccattgat cgctgcataa ttactttttt ctgttcttta tttcgctggt
     1741 ttaatagcgc atggtggaat tttttgactt gtgaatcaat tgtagtttgt attctttctt
     1801 tggtataagg tgcaagaaaa gcacgttgtt tctttatttc attttctatg tcttgtaacg
     1861 ttttagaacg ataattcttg ttggtagtct gctcctcttt caacgtgttg gtcatggttt
     1921 cttcgagtcg gtctaatttc cggttgttca gtcgtaagag ttcgttgttc tcttcgtact
     1981 tcttttgcag tttcgcgaat gctagctcga gctcttcgta gctcgttggt tcgttgttga
     2041 ttgtaggctc gttctcgtct gtccagctca tgtaaaatcg tctccttttc gtaatccttt
     2101 cctagtttgt tggatcgtac ttttttcttt ttgtggatca attcgtaagt aaaatttttc
     2161 ccacgattca agcaatgaat gccgaatgtt ttaagagaat cacaaaattg gttatacgtt
     2221 cgagtttttt tgtttttcat tgtttgatca atcgcaaacc gaatttcgtc tttccaaaca
     2281 ggttcatttt tttcttttaa tttgatttca gacatggttc gtttttcggg tttcttccct
     2341 cgttcagtga ttgttaagtc atgttcaaga caaatctcgt catttaggtc ataacatttg
     2401 tttaaaaata cttgaccatg ggcttgaaat ttgttcccag tttccagatt gacggagtta
     2461 atgacaatgt ggttatgaat atggtctttg tctgtatgtg tgtaaatcgc gatttggtgg
     2521 tcaggggcta tttttttcgc atactctgcc cctagttgat tggctttttc agcggtaatt
     2581 tctcctggtc gaaaagactg gatcaataag tgtgcttgta cacgatcgtc ttttccatac
     2641 agcatgcgaa tttgtttcat ttcgcttttg gcataatcaa tgtcacagtt tatccctgtt
     2701 ttgacggttg ctcgtggttc ggcgtaattg atcgctcggc tacaagaagt ggttcggctt
     2761 actttaactg ttgccatatc ttttgtagct ccttttgcat gatttttagg ttttcggata
     2821 agcgttgggc gtcgccagga tcaacgtcgt gttggtgtgt gttgcaccac ttggcaattt
     2881 gattgatatt cccgccagct ttggctaaat cgcgcgcgat tgcctgagca atgtctggcg
     2941 ccactttcgg tgtcaccaga cgcgcgtttt gtgccttggc tttcacgaaa gccggcacac
     3001 tcatttgcaa agcttcagct gactgcttca gctttgcaaa ttcggattcg ctcactcgaa
     3061 aactgatttg ttttggctcg gatcgatagg tatatttttt agaatgattg ctagccaaat
     3121 attgattttg ttcgctcata agtcccaccc tttcagtacg tcctacggac gtatcgttcg
     3181 caataccatt gtacctaaaa aggtacaata agtaaagctc actaaagctc actaaagggc
     3241 ttggcaagct ctatatttgc tagccaaata cctacgtatt tggtgcaaat aaccgcttgt
     3301 caaaggggcg caagctcccc cttgaatccc cctccccccc actctccaca atacaaaaaa
     3361 gactagcaaa tgctagccac tttttgtaaa gtttcgcttg tttcaccttc ggtgaaattg
     3421 ttgcaaatca ataattaggt agagctacat ttttttcgtt gttcaaagat tcttatgcca
     3481 tttactatta ataaatctat aaatatgata atttttcggc tctataattt atgggactga
     3541 tgaatcagaa ttgattcttc agtcccattt tcattttatt ccaaagaatt aactccaaat
     3601 accaatagaa gcccatcctc tatatacttg agattcctta ctaacaattt cttgtaattc
     3661 tattaaagat ttaggtaatc caaatttatg ttttaaaata tatcttccaa gaaaactact
     3721 taatttcata ctaacttcac caacactttt tccctcactg atttctttag aataaagaaa
     3781 gagcttttct tttaagtcag gatagtcatc tatagttgct tcattgtata ctttactaat
     3841 tgcatttaaa agtttttctt ctttagtaaa atccattata ataacctccc taccactacc
     3901 ttctaggtgc ccatggacca tgattaaccc aaccattaac aataattttt ccaatttctc
     3961 ctttagcttg attccaatct acccaacatt tttctttgtt acaataaaga ccatttccat
     4021 agtaagtagc tgcatcaact tttattcctg taccgatgcc agctagacaa gttcctaata
     4081 ttcctagaat aacacaatgt tttaatactt tctttttcat gattgttttt ccttttcgtt
     4141 tttttaaaat tgcttgttat attaaataat cttagtaatt ttattatata acattttatt
     4201 atataacaat gattaacgtc ataaaaagta gttttgaatt cataatcgtt taaataaaat
     4261 tataagatca gttatttaaa cagctagtag acataatgtg tatactacca agtaacgcct
     4321 gtaccaaggg atccaggcgc tgattttgtg aatttttatt ggtctaggtt aaaagctctt
     4381 tttaaggctt ggttttctgc ggttgtttct ttctaattct ttgtgaatat tgatcggtca
     4441 ttttttaaat acttgttatt tttgtttgtg tttgttatgc tttttacata tctcatattc
     4501 atttattatt cttttctaag gttaataata ggataatctc tttcagaaag actgctcgta
     4561 cgagcagtct tttcttattt atcaagtttt tctacaaata cttcctcatt ttgactgttg
     4621 tagtaattag gattgagttt tagttcccct tttttatttc ctttgttggc cactgataat
     4681 aacattgggc taaagcttaa tattcctagt aggatagaac cgaatagcaa gtgttttttt
     4741 gtcataagta tttccttctt catttccata gctgccacca ttttttccaa ttctttgttt
     4801 gttcgtccat tgtttctgtt gcttcagaaa gagtcgttga actattctct gaagactctt
     4861 ccgtataagt caactgcagt tgctcctgaa gtttttcgat ttgttggttg gcttgcaggg
     4921 tgagttgttg ttgctggtcg agtagttttt gtatatgaga attattttgc tctttgaggg
     4981 caagttgttc tcgtaaaaat tcattttctt cacgcaactg atctagcaac ttgtttgcaa
     5041 ccacgttttt gtttggtata taagcatttt tatcgttgtt ttgttcatgt ttatgcaact
     5101 tatctagcaa cctttgattg tctaagttgc gagcaagttg caatactcca gaacgcaata
     5161 caaccttata accacgctca tttttagcta tcatggaaga atctaatttt tggatatgtt
     5221 tcgatattgc ttgcctactc atcttaaaaa gatctgacaa ttcctttatt gtaaaggttt
     5281 cttgtggttg catactggtt gcctcctagg ttgctactgg aataaattat ctaaatcaga
     5341 taataaactt tttttctctt tttccgaaag tgcggactct gtttgttttt caagagcttt
     5401 tttctctttt tctagttgtt tgatttgttg gaggagttca agttctcgtt tcttctgttt
     5461 gattttttcg ttaattgttg tagcgggttc ttcgtattct ttttgtttat cagctaaagc
     5521 caatgcttta ttttggtctt ctttggttgg tgcatgtttg ttaaagtcgt ctgctttttt
     5581 gatctcaggt ttgaacgtaa attgatagcc aataacaggt tttccccgtc cttttccgta
     5641 tttctttttg attgttagtc ctttgaagat tgcagttagt tcttgacgta taggttttat
     5701 aactcgttta tctatatcag gttgcttata acttttagga atatctaata attcaagaaa
     5761 gtcttctttg gagaaatatg cgaaaccttt ggttctgtat tgctttaaca gacgaaacat
     5821 agtctttgca tagccacttt gtagctcgtt gaattgttgt aaagaatagc gtacccactc
     5881 gtctaaatta tttaacaaag gcagtgcttt ttcgtaaatt cgtatatcta cgtaaggatt
     5941 ttctacgtcc ccgttgattt taaattctgt aaacattaca aacatatcgt aagaaagccc
     6001 agactcactt ctacgaccaa agcgtaaaga taagattttt tcataagttg cttttaaatc
     6061 ttctacaaat cgtacatttg cagttgcttt gtagttactt aaatctttta gttggtcaaa
     6121 cgtaaaacga actgtcttat cccctttttc acgcattcta gacacgatgg
//
LOCUS       DAZUMM010000112         5698 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000112 DAZUMM010000000
VERSION     DAZUMM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.116"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..1851)
                     /locus_tag="ACG8E6_002474"
     CDS             complement(76..1851)
                     /locus_tag="ACG8E6_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFS5062836.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPTVEIIIA
                     CLLAIYYRKYNSSKKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFDFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLKLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVIGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIAVTSMLVFSKLNLKKALSSK"
     gene            complement(2062..2964)
                     /locus_tag="ACG8E6_002475"
     CDS             complement(2062..2964)
                     /locus_tag="ACG8E6_002475"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFS5062837.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3243..5588
                     /locus_tag="ACG8E6_002476"
     CDS             3243..5588
                     /locus_tag="ACG8E6_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFS5062838.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKDEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
BASE COUNT         1807 a         1132 c         1090 g         1669 t
ORIGIN      
        1 ccgcagatgc aagcttttga gcaccgttta ttcggaaaaa ggaactgcga caagactttt
       61 gtcacagttc cctttttatt tggaagataa agcttttttc agattcaatt tactaaaaac
      121 tagcatgctt gttactgcga tgaattgcgt taatggcacg cctgcgccag tgtagacact
      181 taaaaaataa tccttttttt ctggcgcata agaacaaaac cacgagacac cgaaaagaaa
      241 aaccaattgt gtcagtatta atccgatgac tacaatcaaa gaggaactat aataggcacg
      301 atcagctttt gcaaatacgg ttcctagtat cataagtaat atccctgcta ttgaagcaat
      361 cagcacggga atggccagaa cttggtaaat ttttatcatg acattcgcga cttttgctgt
      421 ttttgtcaac tggaaatcag gtgttctcgg atcactgcta tcgatccaat tatccattag
      481 tggaagcttc aataattctt gtgcctgttc gacttgttct tgcgtcccaa aactataatt
      541 ccccccttga tggaaatctt tgtatcgcca tgcataagca tatcctgatt tcagaaaatc
      601 aaatacgatt ggcagatcat tcatcgtctt tccatcccct gatcccatca gatagaattc
      661 ttttttcttt ttgatagttc ccttttgata ggcttcttct aattcttgat tgactttttt
      721 ccaaaaatca ttggtttctt ttgcattgtt ttggtaataa ccagattcgc ttaccacatc
      781 tcgcaatgcc caaaaaatga tatctcctgg taattctttt acttttccgc cttgggtcca
      841 cgcatgagtc gtatacaatt cgtcaatacg agtagaaaca gtatgaaatg taggagatgc
      901 gtctaaagct ttatttaatg cagcatgcga gacccatatc tcactgtttt ctttatttaa
      961 gtttgtttcg tcatctattc ggatcagctg tttcatcaaa cgtccaaatt cgccactcga
     1021 acggtcattg atggctgcaa taccatatgt tgtctcgttt ttatattcga tccactgata
     1081 gacgatggag aaaccgacta gagggagtaa aaacaatgaa agtttcagcc attcttgttt
     1141 cgaaaggaga aatggtcgtt cttttaactt ctttgcttct ttgaaagaaa agtcttgtcc
     1201 tagcaagatc tgaacgaaac tgatgcctaa cgcgactaca gtaaaaggaa gaagccagat
     1261 ggaatcttcg cgaatgaacc aaaagaaagg aaaagaaaca gctagcccta aaaaccacgg
     1321 cagcaatttt ttgctggaat tatatttgcg gtaataaata gctagtaaac aagcgataat
     1381 gatctcaaca gtcgggatca cgagagcatt acgatatgtt cgagtggaat attcatgtgt
     1441 aaaactaatc ggagaaaaca aaaaataaaa atagatactt aaaagaagat acttgttctt
     1501 cacgattggt ttaaacgcta gtgcaaccaa tatactggca agaatactga acatgccgaa
     1561 taaaactgca taagaaagat gcagtttatg tgcaagtact aaaaacaagc tgtaggaaat
     1621 cccttttgaa agtgtcgtag tcgtgtactc ccctagccat ttaccatcca aaatattttg
     1681 agcatagtga atcagcagct gatcatcata ggtcgccgac atatctgttc catagattgc
     1741 tttactatat aaatacaacc gaataattgt aaataatacg gctatgccaa gaataaagta
     1801 tgtatgtttc ttttttagtt ggtcgatcaa ggggtttaaa atgttcagca tcttttcgtc
     1861 ctttcaatca cgttttgcta cttgtttgtg aaacttttcg ttttttactt ttctatcagt
     1921 ataccctgtt tgtctgaatt taaaaagagg gggcaaaagg agcatggcga aaacgaatgc
     1981 aaaaagcact cctttgtttc ttaaacaaaa aaggacaagg cagactgccc aatccttttt
     2041 tgttgctgtc aggtagaaaa cttaaagtgt gacttcttct gctaataact cagggaacac
     2101 ttcgggatcg aatgcttgcg ctttgaacat ggaaatttct tttccatatg gtgcgtaagc
     2161 tttcttttta tctttaccga catatggggt ttctaatatt ttcgaaatgt tgtcaaacat
     2221 tggatcatgg acgattttgt tcaatgtgtc aaatccaatt gttccaaaac cgatattggc
     2281 atgccgatct ttatgagaac cttgaggatt tttagaatca ttgatatgca gtactttaag
     2341 ccgatctaat ccgatgacct tatcaaactc ttccattaca cctgaaaaat cttcttttat
     2401 attatatccg gcatcattcg tatgacatgt atcaaaagta acggataatt tttcattcaa
     2461 ttttactcca tcgatgattt gcgctaattc ctcaaatgtt cgaccgattt ccgtaccttt
     2521 ccctgccatc gtctccaaag cgatctgtgc cgtctgttct tttgtcaaaa cttcattcaa
     2581 cccctcaata attctagcaa tcccagcttc tgcccctgct cctacatgag cgcctgggtg
     2641 aagggtgatt tgagtagcac ctaaagcttc cgcacggaca atctcttctc tcagaaattg
     2701 tacagcaaat ggaaacattt ctggtttgat cgtatttccc aaattaatga tataaggcgc
     2761 atgaacgacg atatttgtta gtccgttttc tgccataaat gcttttccgg catcaatatt
     2821 catctcttcg atagcttttc ttctagtatt ttgaggagcg cctgtataga tcataaaggt
     2881 cgaagatccg taactggctg cttcttctgc cgcgcctaac agcattttct ttccgctcat
     2941 gctgacgtgt gagcctaata acatggatgg attcctcctt gaaaggcaat tgtttcgaac
     3001 aattcctgta tattgataag atgatgtaca aagaaaatag tttgtttttc tttgccctgt
     3061 tctccatagt aactgaaact ttccctaaag aaaagcttaa gaactctttc ataaaaaaga
     3121 tcatttcctc acgttttatc tcattcgaaa aagaaattcc tgtcaaaaac agccttgtct
     3181 gtttttttgt cctattattt gctataatta gcgaaataaa accattagtt gaggtgaccc
     3241 ttttgccacg caaaaatact cgaaaaaaac agaatagaag aaaaaaagaa aaatggtttg
     3301 ttcctaagat tatttttaga gtgtttcaat cattgactgt ttttattact gttcttcttt
     3361 tgttgttcgc agctctaggt atcggaatag gtgctggcta ctttgcctat cttgtcgaag
     3421 acacacaatt gccgactaaa aaagaactgc agacagaact aggaaacatt acggaaacgt
     3481 caaagattgt ttatgcagat aatacagaga tatcaaaaat ccagacagat ctgatgcgga
     3541 cgactatttc atctgataag atttcgcctt tattgaaaac cgcgattatc agtacagaag
     3601 atgaatactt cgataaacac caaggttatg tccctaaagc tgttttacga gcactagttt
     3661 cagaagcgac tggcatcggc tcgtctggag gatccacttt gacacagcaa ttagtcaaac
     3721 aacagatcct aaccgatgaa acaacattca aacgtaaagc aaacgagatc gttctttctg
     3781 cacaagtaga aaaacacttt tcaaaagatg agatcattac tacgtatttg aatgtctcac
     3841 catttggtcg gaataataag gggcaaaata ttgctggagt ccaggaagct gctagaggaa
     3901 tctttggtgt agatgcaaag gatgtgactc tgccgcaagc tgcttatatc gctggtttgc
     3961 ctcaaagccc tatcacgtat agtccataca cgaatacagg cgcgctaaaa gaagatcttt
     4021 ctgccggtct tgccagaaaa gattttgttt tattcagtat gtaccgtgaa ggacaaatta
     4081 cgaaagaaca atacgaagaa gcgaaagcct atgatcttac aaaagacttt ctcccacaac
     4141 aaatcgcgga acagactgac agagaattct tgtattatac cgtgatgaac gaagcaacaa
     4201 ggattattgc acaacaattg gcagaaaaag ataatgctga tatgtcagat tcatcggtca
     4261 gtgaagctta ttatcaaaaa gcacagcaaa cgatccaaaa caaaggatat acgatccatt
     4321 cgacgattga taaagatatc tatgctgcat tgcaaaccgg cgtagaaaat tatggctaca
     4381 tgttagatga tggtgctggt agccaagtgg agactggaaa tgtattgatg gataacagaa
     4441 ccggacgtat ctatggcttc gtgggtggtc ggaattacag ccagaatcaa aataaccacg
     4501 cctttgatac agaacgtcaa gctggttcat ccatcaaacc ggttcttgtt tacggtcctg
     4561 caatcgatat gggacttgtc ggatcagaat cccgtgtatc agattatgca acgacttggc
     4621 aagaaggaaa aaatgctggt gagaaaatcg tcaatgcgac caatgaaggg tcgaacacct
     4681 tccaaaccgt ccgtgagtca ctggaatggt ccaacaatat cccagcttat cacttatatc
     4741 aagatgtgtt gaattccggt ggatcgaaac aatacgctta cgagcactac ttggcgaaga
     4801 tgaactaccc agcaaatgat aattggggcg ttgaatcggc tcctttaggg acagtagatg
     4861 taacgacgct tcaacagaca aatggattcc aggccttagc aaatggtgga gtctacgaag
     4921 aaggctatat cattgattcc atcacagata atgcaggtaa tgtgatttat aaacatgaaa
     4981 gtaattctgt ccgtatttat tccgaagcaa cggcatcgat catgaatgac atgatgcgta
     5041 gtgtgatcaa tgaaaaaatc actacaccat tcaaagatgt catctcaagc ttaaacggaa
     5101 atctaggaaa tgcagattgg gtcggaaaaa ctggttctac caatgaatac cgagattcat
     5161 ggctgatcgt ctcaactcca tctatcacta tcagtagttg ggcaggacac gatgacaata
     5221 ctggaatgga cagccaagca agaatacgtt cggctaatta tcttgccaat ctaatcaatc
     5281 aagcttacca agcaaaccct gagattttcg gcactgatga gaaatttgac ttatcatccg
     5341 atgtgatgaa gaaaaaagta tctgggttca caggacaaac agctggaaaa gttactgtca
     5401 ataaaaaatc tatccaaacg ccaggaaaaa cagtggaatc actttgggca aaaaatggtc
     5461 cgaagaaaag cgcctttaaa ttcggcgttg gcggtactga tgaaaactat acagattact
     5521 ggaacaccgc ttcagcttat gctagagcga atccagagac caaagaatca gacgataaag
     5581 atgattgatc taatagatta atcagaatta aagggagctg cgacaaaaat cttgtcacag
     5641 ctcccttttt ccgaataaat ggtgttcaaa agcttacatc tacgatatta agacaaat
//
LOCUS       DAZUMM010000113         5694 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000113 DAZUMM010000000
VERSION     DAZUMM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.117"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            249..674
                     /locus_tag="ACG8E6_002477"
     CDS             249..674
                     /locus_tag="ACG8E6_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5062839.1"
                     /translation="MHLHRISALAVQIGRYHHSETRAVMEGLEEAIQTTNDCPYRRTF
                     HSDQGWAYQMKAYRNKLKEYKIFQSLSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCF
                     EELKSAIDSYIYYYNNERIKQKLNCQSPVQFRKTTVTVV"
     gene            complement(979..1941)
                     /locus_tag="ACG8E6_002478"
     CDS             complement(979..1941)
                     /locus_tag="ACG8E6_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5062840.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGESI"
     gene            complement(1943..3382)
                     /locus_tag="ACG8E6_002479"
     CDS             complement(1943..3382)
                     /locus_tag="ACG8E6_002479"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFS5062841.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            3567..5513
                     /locus_tag="ACG8E6_002480"
     CDS             3567..5513
                     /locus_tag="ACG8E6_002480"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFS5062842.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFVFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVYSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1826 a          978 c          948 g         1942 t
ORIGIN      
        1 ataaataact cataaaaaat attttgactt tcaaaatcac ttttgctacc agaagttgag
       61 gcgaagtgtt ttaagcatag ttgtacccta gtttgaccgt taaaaataaa taacgatcgc
      121 tagagtgcaa ctatgtttgt ttgctctacc gtcagcgtcg gcttggcctc tcacatccat
      181 taccgtcggg atcatctccg ttacataccc ttacaaaata cgccctgtaa gcattgcatg
      241 aaaccaccgt gcatttgcac cgtatttccg ccctagccgt tcaaatcggt cgctaccacc
      301 attcagaaac acgtgcggta atggaaggtt tggaggaagc tatccagacc actaacgatt
      361 gtccctatcg tcgtacgttt cactcagatc aaggttgggc gtatcaaatg aaagcttata
      421 gaaacaaatt gaaggagtac aaaatctttc aaagcctgtc tcggaaaggg aactgcttag
      481 ataattcact tatggagaat ttttttggct tattaaaaca agagattttt cacgggaaag
      541 tttataactg tttcgaggaa ttaaaatcag ccatcgatag ctatatctat tactacaata
      601 atgaacggat aaaacagaaa ttaaactgtc aaagtcccgt ccaatttcgt aaaacaaccg
      661 ttacggttgt gtaaaaatat cattttagag catgaaaaaa ggagtggatt tctccactcc
      721 aaaaaagtct aacttttggg ggtcactaca tttttgatcc tttttatttg attaattata
      781 tccagctgga taataatttt gggggcaaca tttctatctt actattatct tcctaaagta
      841 gtagactttc ttattgggaa aaattacaac aaaaaggagt ggcttagttg acaactcctt
      901 tttgtttaca aatcaattgg atatctacaa attgtatttt ctaacggatt ttatagacat
      961 taaaaaatta atttgacgtt aaatactttc tccagaaagt attgagattg gtaaaatata
     1021 atccttattt gtcttttcgc ctttaatttt tttcagaaga atatcaacaa taagacttgc
     1081 aatttcatca attggttgtc ttattgttgt caattgtggg aaaaatttct caatgaaaga
     1141 ggtaccatcg tatccaataa tttttaaatc ttctgggata ttaagtttaa gttgatgagc
     1201 aattttcata gtgagaatgg ctgttaaatc atcagaaaca aaaataccat ctggtttgtg
     1261 atttataata gttgacttta tttccatttc acgacggata gtagacagat tattaggaat
     1321 tttgaaaatt tttccatttg gattttgaaa agaaaaacct agtgcacgta attctgtggg
     1381 agaatccgtg ttatcatttc ctgtaatcat aatggtattt tgacaaccat ttttttgtaa
     1441 agtcttagct gcaagttttc ccccttcaaa gttatctgaa gaaacaatgg aaacattagg
     1501 agctaaattt ctatcaaaag caacaatagg tgctctcaca cgctcataat cagcaattcc
     1561 aagattatgg cttgatgaga ttatgccatc aacttggttg gcttcaagca tttcaatgta
     1621 ttctctctct ttagcaggat cattttgact attgcaaata atcgttttat atccatgttt
     1681 aaataactcg atttcgagat actcaatgag ttcagagtaa aagatattac taatattagg
     1741 aaaaataaga ccaataagct gaggtgactt tccttgaagt ccacgtgcta aattatttgg
     1801 tttataacca agagttttca tagcttcatg aactttttgt tttgtttttt ctgataaata
     1861 tcccttatta ttaatgacgc gtgaaacggt tgttgggcta actccagcta attcagcgac
     1921 atcagttaat tttgctacca ttttaatatt ttaattcaaa gtattttcca gtaggttcgc
     1981 ctgatttgat ttgaatacca ttttggtgat tttcagggaa tacacgacta gtaaacactt
     2041 tttctccttt attaataaaa atctcaatga ttgagttatc tacaaaaatg ttagcagttg
     2101 tttctttagt atcaattgag cattcacgaa tcgtaccaaa gtcagtggca tattgaatgc
     2161 ctgctttgct gcgatctaaa ataatttttc cttctttggt atctacaatc aagctaagtc
     2221 catttccttt actatcagag aaaagaagta actccgattt ctgatttgga ggaaaagtta
     2281 gttcaagctc ataagtatta tttgtttctg cttttgctgt aaattgttca gaactagcac
     2341 gtaatgatgt aatagcttct actggatatt ggtaaagttt accatctttt attgaaagtt
     2401 cttttacaag actcattgct ccctgataat catacttatc agttggataa tcgatatcag
     2461 gtagtccaat ccaactgaca atcagagcac gaccatcagg agtattgaat ccttgagtcg
     2521 catacgcttc aaatccataa tcaagatttt gaatttctga agcgcccaca agttttactt
     2581 tctctatgtc aagcgattga cagatcttaa aagtattcgg atagatattc ccataattta
     2641 gctcagattt atccaaccct tgaggacaat aaatgagaac aggttttttg tctacaaata
     2701 ctagatttgg acactcaatc atgtattctg aaccagtgcc gccaaaatca aggttgccaa
     2761 cttcctccca tgtttccacg ttattgtcat ccgctttgta aagtttgata aagcctgatt
     2821 tttctaaatt ttgtgctcca acaatcgcat ataattgtcc ttcataatta aacatttgtg
     2881 gatcacgaaa atgatcagtt acatcagcag gtttatgaat aagaacattc tcacctttcg
     2941 taatgttgta gtctttatcc atccatgctc cgatttgaag tgtatcacgt tcccaatttt
     3001 catttcgaac gtttcctgta tagagtaaga atagattccc ctctatttca tatgcagaac
     3061 cggaataagc gccgtggcta tctcgttttg tgtctggaag aagttctgct cctgttacat
     3121 gaaaatgaac taaatcatct gattcattgt gtacccattc tttcaaccca tgagcagctc
     3181 caaatggcca attttgataa aaaagcgtat attttccgtt aaaaaaagaa aaaccattag
     3241 gatcgtttaa caatcctgtt ttaggttcga tatgatagct agcacgccaa ggtgatcgtt
     3301 ttacattatc cttaatttta tccatctcat ctttagtcca atcagaataa gcacgatatc
     3361 gtatttctcg aggtaagttc atattttact ccttcaattt atctttttac aactagtatg
     3421 gtaaacgttt tccgttgaaa aatcaatcat ttaatttaaa aaaataaaat atatgacaaa
     3481 cgtttgacat atttttaaga tatgctaaaa taaatttgca tttcagtgaa ccgttttcac
     3541 gtaaaaataa aaaaggagtg attcgaatga attacagtaa agtagcttcc gacgtaatac
     3601 aagcggtcgg aaaagataac cttattgcag ctgcacactg cgctacacgt cttcgtctcg
     3661 tactaaaaga tgacacgaaa gtgaatcaaa aagctttaga cgaaaatcca gatgtgaaag
     3721 gcacatttaa aattgatgga caatatcaag tgattatcgg tgcaggtgat gttaatttcg
     3781 tttatgacga acttatcaaa aaaactggac tttcggaaat ttcaacagat gatttaaaac
     3841 aaattgctag taaagataaa aaattcaacc ccattatggc attaattaaa ttattatcag
     3901 atatttttgt tccaattata cctgcccttg ttgcaggtgg tttattaatg gcattacaaa
     3961 actttttaac agctgctgga ctttttggac caaaatcaat tgaagaaatg tatcctgcga
     4021 ttgaaggtat ttcctcaatg atccaactga tgtccgctgc accattcatg ttcttaccaa
     4081 ttttagtagg tatttcagct gctaaacgtt ttggagcaaa ccaatttctt ggtgctgcaa
     4141 ttggtatgat catgacaaca ccagatcttg gtggctcaac tgaatattgg aatatttttg
     4201 gttatcatgt ttcacaaaca aactatgctt atcaagttat tccagtttta gcagctgttt
     4261 ggatattatc tatattagaa aaattctttc ataaaaaatt gccatcatct attgacttta
     4321 cattcacgcc attattatca gtaatgatta ctggattttt gactttcaca gttattggcc
     4381 ctgtaatgtt attgttgtct aatggaatta cagacattat tgtttggcta tataacacta
     4441 ctggatttat tggaatgggt atttttggag gaacatattc acttatagtc atgactggtt
     4501 tacatcaatc atttccagct attgaaactc aattactttc agcgtggaca aatggtatcg
     4561 gacatggaga ctttgtcttt gtagttgcat ctatggcaaa cgttgctcaa ggtgctgcaa
     4621 catttgccat ttggttctta actaaaaatt ctaagacaaa aagtcttgct tcctctgcgg
     4681 gtgtctcagc acttcttgga attacagagc ctgccttgtt cggagttaac ttaaaatacc
     4741 gctttccatt cttttgtgct ttaattggtt caggaattgc tacagcagta gcaggattac
     4801 ttaaagtagt tgctgtttca cttggttcag caggtttctt gggatttctt tcaattaatg
     4861 caacatctat cccattttac gtattgtgtg aattgattag ctttgtagtt gcttttgcaa
     4921 ttacttactt ctacggtaaa acaaaagctg ctggtatctt taaagcagaa gaaattgctg
     4981 aacaaacacc agttaattct tcaaaagtag caacaacaga tccaatatct actgatgttg
     5041 aatcaacaca aacaaaagtt tcatcagaaa caattgttag tcccatttct ggtaaagtga
     5101 ttagtctttc ttcagtaaat gatccagtct tttcatcaga agctatggga aaagggattg
     5161 ccattaaacc aaatggaaac acggtctatt ctccagtaga tggtactatt caactcacgt
     5221 ttgaaactgg acatgcttat ggtattaaat caaataatgg tgctgaagtt ctaattcata
     5281 ttggtattga tactgtatca atgggaggaa aaggatttac acaaaaagtt actactaatc
     5341 aaaaagttag aaaaggcgat attcttggag catttgaccg ttcaatgatt gagaaagaag
     5401 gtcttgatga tacaacaatg attattgtga ccaatacttc ggattattca gaagtaaata
     5461 tagtagctga aaatacagtc actgagggta ctgatttact tgaaatcaaa taaatgtatt
     5521 tccctgagta aagatggaat tttctttcca aaatcaaata atatagatta atcaaatatt
     5581 tgagaataac attttattta atcttttcat atatcaattg tatgaaaaat tatcagaatt
     5641 ttaaaaaatg ataaaaattc aaaaagaaaa aatagtagtt atttacttgt catg
//
LOCUS       DAZUMM010000114         5460 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000114 DAZUMM010000000
VERSION     DAZUMM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.119"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..654
                     /locus_tag="ACG8E6_002481"
     CDS             <1..654
                     /locus_tag="ACG8E6_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062843.1"
                     /translation="SSFSAFLLIACLTLYHSQSALANQVQDRLSFIGASASTELLPAL
                     ELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNKQELINWRLTGLSKTKLFLFIFWQL
                     FFPLFLCCILVLLWIVSFQSVYEALLQRINFLVLKTTELPDIRDILTSVSGLTIPMDQ
                     QTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLILSLFPFTFFIHHLQSRRPGRNET"
     gene            644..1153
                     /locus_tag="ACG8E6_002482"
     CDS             644..1153
                     /locus_tag="ACG8E6_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062844.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1223..2671)
                     /locus_tag="ACG8E6_002483"
     CDS             complement(1223..2671)
                     /locus_tag="ACG8E6_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5062845.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2671..3387)
                     /locus_tag="ACG8E6_002484"
     CDS             complement(2671..3387)
                     /locus_tag="ACG8E6_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFS5062846.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3368..3718)
                     /locus_tag="ACG8E6_002485"
     CDS             complement(3368..3718)
                     /locus_tag="ACG8E6_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFS5062847.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            3862..4635
                     /locus_tag="ACG8E6_002486"
     CDS             3862..4635
                     /locus_tag="ACG8E6_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5062848.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            4806..5172
                     /gene="ssrA"
                     /locus_tag="ACG8E6_002487"
     tmRNA           4806..5172
                     /gene="ssrA"
                     /locus_tag="ACG8E6_002487"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1579 a         1079 c          959 g         1843 t
ORIGIN      
        1 tcaagctttt ctgctttttt attgatagca tgcctgactc tttaccattc gcaatcggct
       61 ttagccaatc aagtccaaga tcggctttca tttattggcg cttctgcatc tacagagctt
      121 cttccggctt tagaattagc tagccgattt tatataaaag gcatattcag tttatttttt
      181 ctatttgcct tcctttttat ctttttcttt tactattcag tcaggcaaaa caagcaggaa
      241 ttgatcaatt ggcgcctgac tggtctttct aaaaccaaat tattcctttt tattttctgg
      301 cagctgttct ttcctctctt tctttgttgt atcttggttt tattgtggat cgtcagcttc
      361 cagtcagttt atgaagcgct tcttcaaagg atcaattttc ttgtcttaaa gactactgaa
      421 ctgccagata tccgtgatat cttgacttcg gtaagcggac ttacaattcc gatggatcaa
      481 cagacttttt ttaccatcga tttccaaaat gatctttttt tcacagatat acttaaggga
      541 tttgcccaaa ctcttattgg gctcagtgga tgctgtctca ttcttagtct attccctttc
      601 acttttttta tccatcattt gcaaagtagg aggcctggac gtaatgaaac ctgaatattt
      661 gattcatgct gaacacttga ccgttatttt gtgtcctctc gaaaaacagc ataaaatatt
      721 ggagcacttg aaaaaagagg ttcttgttgc aaaactggat aattttgcga tcatccaaaa
      781 aaattggccg atgtttcctt atctgaatct taaagaccat gtcctgttgg atgtaccgga
      841 aaaagaaatc aaacaagatc gtttggccta tcaagaaaaa cttgatatct ctccttcctt
      901 gcttaattgt gcggcagctg agttgacgtc ttttgaaaaa gtaaagctgc aattacttca
      961 tgccttgctt tccaagcgaa acaatattgt tattgaagat acatttgatg aacttagtgt
     1021 attggagatc caagagctac tccacttgct ttcttattta gctcatgaag aaaatcaagg
     1081 tatcttactc tttactcatg atgctactat tgctcaaagt ccttatattg atcgtttaga
     1141 gcccgcgggc taaatcaatc attttatagt aaaaacaagc ggtatagaag atcgattcat
     1201 gatcttttat accgcttgtt ttttactctt ttttgatttc tacgtcaggc aaacgaacag
     1261 cgaattttgt tccttttcct agttcgcttt ctacttctaa ttttccttta tgcaagcgta
     1321 caagttgctg cacgattggt aaacctaatc ctgattcacc atattttgta ttttttcttg
     1381 aaggatctac tttataataa cgatcccaga tacttttcat ttgttgttca gacattccaa
     1441 ttcccgtatc ttcaatcgtg atgatcgttt caagatagcc tttttctaaa gtaatcgtga
     1501 tttggccgtt ttcagtaaac tgtatcgcgt tttgaataat attgaccatg atttggacaa
     1561 atcggtcata atcagcgtaa acatcaatat catcaacggc tttcaatgtc aatgtatctc
     1621 ctgctgcttc tgctttagct tctaactgtg caacaatatt tttcaatgtt tctgttccat
     1681 tgaatttttt aatgaccata ctgatttgat tcgtacggat tttttcataa tccaagtttt
     1741 cattcaccaa acggatcaga cgctctgttt catttttcat caatttaatc gcgttttctc
     1801 gctgattttc agggattgca ttgtattcta acccttccag aagtccgttg attgtcgtaa
     1861 gaggtgttct catctcgtga gaagcatctg ccataaattg tcttcgacga ttttcttgtt
     1921 cttcaatttc tgcttgagat tctttcaatg aattcgtcat tttattgaaa tcatctgcta
     1981 actcatcaaa ttcatctttg tcatgtacgg ggagctgtac atcaaaatta ccattcgtca
     2041 cttctttcgt cgcattcctt aaccgattga tacgcttaac ttggaatgct gcaaaagcat
     2101 agctggcaat aactgcaacg atactggaaa aaataaatcc tttgaacagg ttcagtgtaa
     2161 ttggacgaac gctgttatcg atatttcgcg caggttgact aactacaagt gctccataga
     2221 attcatgatt caaattaaat ggcacaagtg cgtaagaggt cgcctgtgct tgtcctaaaa
     2281 tatttttatt cgatgtgaat ttttcttgac gtccattcct cagatttttc cattgatcag
     2341 ctgttaaggt aaaattcagt aaagctgtat tagtcggata aatgacacgt tcgtttttat
     2401 cgataaatat aaagttcaca ttttgttctg tcagtacttg ctctgtcagc agcaatgcat
     2461 tttgaaacgc ctgatcttga ctaaactgcg ggagcgtatc ggcataggtt tgcgttgtct
     2521 tttcaactga ttcagcatat ccaaacagtt gctggtaatt gttttcttca atcgtttgtt
     2581 ttgtcagttg tgtaaaagaa actccaacga tcaataagat caacgcgacc attccgcaaa
     2641 aagctaagag ttgctgatac aaataacgca ttaggcaaca ccagaatcat cgaatttgta
     2701 gccgactccc caaacagttt ggatgacttg tggtccgacc ttttcgattt tttgtcgtaa
     2761 tttcttgata tgggcgtcca ctgtccgttc atcaccaaag tactgataat cccagactaa
     2821 ttcaagcaac tgctcccgag agaatacttg acgtggtttt tttgctaaag taaacaatag
     2881 atcgaactct ttaggcgtca gtccttcaat tggccggttg tccagatacg cttctcgtgt
     2941 tttcgtattc atcttgaaat gactagtctc gatatcaaaa tgatcatctg aaactgctgc
     3001 ttcttctctt gcttctgcca actcactacg gcgatgcaaa gctttcattc gagcaataag
     3061 cgtcagagga ctgaaaggtt ttgtgacata atcatctgcc cccatctcta agccgatcac
     3121 ctgatcgctt tcagaatctc tagctgtcag catgataatc ggcacagtac ttgaaacttt
     3181 gcggatctct cttcctacta gcataccgtc cagtgtgggc aaattcaggt ctagtgtaat
     3241 catatcccaa tgttgtgggt tttctaaaaa ggcatcgagc ccttcttttc catcatactt
     3301 gaaagttgct tcccaacctt catttaagaa aaacatctgc atcatttcag aaacagattg
     3361 attatcttca atcattaaaa tattcattat cgttccctcc agtttccagg tcatatttct
     3421 gtaatttctt ttctgtcata ataaaaatag gcataattgg aaatactagc agaagcattt
     3481 caatcagaaa ataattcgta tgcccagatg tctttaaata atagccgatc aatgccatca
     3541 gtaagcccat aagaaaaaac cagtttccgc ttgtcttttg agccagccgc caatgagcgt
     3601 ctgtttttgc ggcaagaggc gaatgatacc cataaaaagg cagatttcct tttgagggca
     3661 gaatccgaaa aagactgcca attattaaga ataagatacc aatactgaaa aaaatcattc
     3721 tttccctcct aaattctctt cttatagtat actctaaatt gttgactgaa ccaatatgtt
     3781 caggaattct tcacgaaagg tttgtctttt gttcattctt tgttaagata aacttattcc
     3841 aataaaaaag gagttttatt tgtgtcaaat tatcgtgcaa tcactttctt tgatcttgat
     3901 ggaacattac tagatgcgaa atcaaaaatc acacccgaag tggcaaaagc tatgaacgca
     3961 ttaaaagaaa acaatgttct ccccgtcatc gctacaggac gtacagaagc agaaatccac
     4021 tctatcatgg aagatgcggg cattacttct gcggtcacca tgaatggttc tttcattcga
     4081 gtagacggcg aagaaatcta ttctcaaaca ttttcttcag aagaatgtca aaaaatgtat
     4141 gatcatgtac agttgaacgg tcatgaactt tcttactata acgatcagaa aatctggtgt
     4201 accggtcata gccaaacaat gatcaatgct tacgacttca ttcattcaga tgttccggaa
     4261 attgatcctt taggctttaa agaaaacaca gtaaatatgt tgctgatctt atctcaatca
     4321 ggtgacgaat actatcacga acattttcct gagctaactt tttatcggaa tggtccttac
     4381 tcgatcgata cagtgaagaa aggaatctca aaaggggcag gtgtcaaacg tctgcaaaaa
     4441 gaacttcagc tggaaaaagt tcctactttt ggatttggcg atggtccgaa tgattttgcc
     4501 ttgttggaag cttgcgacaa caaaatcgct atggacaacg cctatgacgg attaaaagaa
     4561 ctttctacct ttatcacaaa gaaaaataca gaaggcggaa ttgtccacgc attgaagtat
     4621 tttgatctga tttaagatct ttttttgaag ctacctcttt tcttaagaaa atcgcttatt
     4681 ttttcttaag aaaggggcag ttattatata aataatggga tatgattcgc ttttttattg
     4741 aatttatccc aatatatgct atactaattt tgtatgaaaa atacacgtaa cccttgttag
     4801 ctaacgggga cgttacggat tcgacaggca cagtcgagct tgaattgcgt ttcgtaggtt
     4861 acgtctacgt aaaaacgtta cagttaaata taactgctaa aaacgaaaac aactcttacg
     4921 ctttagctgc ctaaaaacag ttagcgtaga tcctctcggc atcgcccatg tgctcgagta
     4981 agggtcctaa ctttagtggg atacgtttca actttccgtc tgtaagttga aaaagagaac
     5041 atcagactag cgatacagaa tgcctgtcac tcggcaagct gtaaagtgaa tccttaaatg
     5101 agttgactat gaacgtagat ttttaagtgg cgatgtgttt ggacgcgggt tcgactcccg
     5161 ccgtctccat atatgtatgg ttgagacaga taaacatgta tgaaaaacgt tgttaaatca
     5221 acgttttttt attttggtca aggagagaaa agtagagaaa tacaaaattt tttgcattga
     5281 ttttgcattc aaaaaaacat tcaaacattt agaaatcccc aatacgtatt caggaaaatc
     5341 tattttaagg tttacaaaaa gaacttttgt tcgtacactc tttttgaggt gaacttttat
     5401 gctgatggaa ggaaaaacac aattatggtt taagcatgtt ccttcaaata agttttttta
//
LOCUS       DAZUMM010000115         5428 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000115 DAZUMM010000000
VERSION     DAZUMM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.120"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(361..474)
                     /locus_tag="ACG8E6_002488"
                     /pseudo
     CDS             complement(361..474)
                     /locus_tag="ACG8E6_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(857..2089)
                     /locus_tag="ACG8E6_002489"
     CDS             complement(857..2089)
                     /locus_tag="ACG8E6_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS5062849.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2344..2913
                     /locus_tag="ACG8E6_002490"
     CDS             2344..2913
                     /locus_tag="ACG8E6_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5062850.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3091..3531)
                     /locus_tag="ACG8E6_002491"
     CDS             complement(3091..3531)
                     /locus_tag="ACG8E6_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFS5062851.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3689..4453
                     /gene="map"
                     /locus_tag="ACG8E6_002492"
     CDS             3689..4453
                     /gene="map"
                     /locus_tag="ACG8E6_002492"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS5062852.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4485..5408
                     /locus_tag="ACG8E6_002493"
     CDS             4485..5408
                     /locus_tag="ACG8E6_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFS5062853.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
BASE COUNT         1690 a          986 c         1046 g         1706 t
ORIGIN      
        1 ctaaaaagaa aacttctgaa ttccatactt tataaaaatc tttgataaat ctattcgaag
       61 ggtcaaattt aaaccataat tgtgtttttc cttccatcag cataaaagtt cacctcaaaa
      121 agagtatacg aacgaatgtt ctttttgtga aggcggaatt taatatactg aataatagaa
      181 aacaagtact tgtgccaaat tatgtgccaa aaaaattcga attcaacaaa atgtaaacaa
      241 atagaaaacc ttctatatta agatttcctg caataacaaa cacaacagaa gacgtgtaat
      301 agttagtcag gaacgtacaa ataacccctg tatcctttgc ggtacagggg gttattttgt
      361 ataagtagac attgtagttt ctaaactgct atgtcctaaa cgtttcgata cgctaagtat
      421 atttactcct tgataaagta aaatagatgc atgcgtatgt cttagtccat caataaacag
      481 tcgaagagaa aatagggaaa aattgattac taaagaaaaa cttactaggt cgacggtgta
      541 gttgattttc atacttcaat agattcgaat taaacgattt cgattggctt ataccagtcc
      601 tatccaacga aagatatatc gttcttaact ctagcggtat aacttgaagc tttacgtaaa
      661 gccaatggga atgaactgga gatgactttt tattattttt agtaatagct gatgggttaa
      721 tacttacaac tttccttttc aaagtaccat ataaaaaaac ggtggggttg tttagcgaaa
      781 taaaggatct aagccaatag cttagatcct ttattctatg ctttatatta ttttttgcat
      841 gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa aatcggtact gtagagccat
      901 cttcacggat tccgtcgata tccatcttgt cagatcctac catgaaatct acgtgggttt
      961 gacttctatt caatccagct tctgctaatt cttcatcgga catttctgta ccgcctttca
     1021 cactgaaggc ataagcagaa cctagtgcta agtgattcga tgcgttttca tcaaataatg
     1081 tattgaaaaa gatgatgcca gattgagaaa taggtgaagg gtcaggtaca agtgcgactt
     1141 ctcctaaacg acgcgcacct tcatctgtat caagcagttt tgctaagaca tcttctcctt
     1201 gttcggcaga aaaatcaaca acttttccat ctttaaaagt aaatttcata cccgagatga
     1261 tggtgccagc ataactcagt ggctttgtac ttgaaatgta accatctact cgatgactgt
     1321 caggtgctgt gaacacttct tccgtcggca tatttgccat gaatttttct ccacgagcat
     1381 tatagcttcc agcgccttcc caaagatggt ttttaggcaa tccgataatg atatctgtac
     1441 cgggagcagt atagtgaagg gcagaaaatt gttctcgatt caattcttct gctttttttg
     1501 ctaatttttc atcatgcttc ttccatgcta agacagggtc ttcttcatag acacgagtcg
     1561 ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc ttctggcagt tctgggaaga
     1621 cttttgctgc ccattgttta ccagccgcag ccacgacagt ccaactgact ttatttgctt
     1681 gagttgcctt gcgtaaattc attagtgctt tgccattcgc tgattgataa gacgctactc
     1741 gatggctatc tacaccagca aatgcatcgg gatcggcaga aacaacgctg atcctgcttg
     1801 ctcctttttc aagccattca tccgcttgat cgattttaga ttgtggtaca ttctcaatac
     1861 gatccgttgc agcatgtaaa agaaattctc tttggatctg gtcgtcagtc cattgaacga
     1921 taacctcagc ggctcctagt ttataagctt cttgtgtgat caatcgagca aggggtgcct
     1981 gttccacgct gatctgtaaa acaactgtgt gtcctttttc tgtggctacg ccagtttctg
     2041 caattaagcg tgcatatttt tttagaagtt cattaaaatc agataacata ataaaaattc
     2101 ctcctcacca ttttttctgg taactattag catgatacca tttaattatg attcaaacaa
     2161 tgaggcttta aataaaaaaa ggaggttatt tttttaaggt taagtttcat gatatatgtt
     2221 actacttgct atttcagctt gtgaagaaaa tcaaaaacta attttgcata tcaaaaaaaa
     2281 catgttacaa tgaaattaat caatggtgga agcgttataa aaaaattaag gagattaaaa
     2341 aatatggcaa gaaaaaagac gattactaga gaacagattt tggcagctgc ttatgaagta
     2401 gctgccaccg atggatttac acgatttact gcgagaaaca tcgcgaacaa aatgaattgt
     2461 tccacgcaac cgatttatct tgagtttaaa aatatggatg acttgagaca agctcttttt
     2521 gagcagatct atgatcattt acaatatgaa gtgttcactg ttgagcatac agggaatacg
     2581 attatcgatc ttgcttttaa ttatatccat tttgcaagaa gagagaagaa gttgtatcgt
     2641 tcactgtact tagaagaata tggtggagga aaagagatgc agaatttctc ttatacttac
     2701 tttgtgaata cagtgaaacg cgatccagct tatgtgaatc tttcagaaga gcagatcgaa
     2761 tctttgtata atgggacatg gatcatagct actgggatcg cagctttgat gtcttcaagc
     2821 atcatccatc caactgatga acagattgaa aagatgatcc aagacagtat agatgctatc
     2881 ttgaaattca aagaaccaat cgaaatcgat tgaatatact caaaagttcc acaaatcagt
     2941 aggtaacaag agatgtgtca aaataaggaa aagttcgttt ttcttacagc gtatgcattt
     3001 ttaactcccg aagaactctt ccatgaatgc aatgaataaa agagccatac agattgaata
     3061 aatctggatg gctcttttat tgcaagtata ttaaattttt gcgactagtt ctttagcgaa
     3121 agcttctaaa ttttcgatat cttcttcttc agcattcaga tccactttaa cattttcggc
     3181 tccttttgat gcgcctgttt ttgcaaaagc agcatcgaaa tcatccaccg atttgcagaa
     3241 ttcatcgtaa aaagtatcac cagatccgca gacaccgtaa atttttccca atagatccaa
     3301 ttcttgcaag tcttcataaa agtctacgat ctcatcaggc agatcgccat catcgtatgt
     3361 ataagtagct actacgcaaa tatctgcctc ctcgaattca tctgcatcta cttgggtaca
     3421 ttcgttgatt tcgacttcga tatccagatt ttcaaatgct tctgcaacaa tatcggcgat
     3481 ttcttcagtg tttcctgtca tgctagcata tacgattttt gctaaagcca tgatgtgcct
     3541 cctcgtttat aattatttaa caattatgaa ttataccaaa acatcattga aaagaaaata
     3601 aaacctagag acacctgggg aatgtcattt ggctaaatgt atgccgctat gttaaaatga
     3661 gagggatttg taaaaaggag acgaaaatat gattacttta aaatcagcac gtgaaattga
     3721 aatgatggca gaatctggag ctttattagc agatgtacat aaaaatctgc gtgattttat
     3781 aaaaccagga atcacaagtt gggatattga ggtatttgtc agaaactata tcgagagtca
     3841 tggcggaatt gctgcgcaaa ttggctttga agggtatgaa tatgcgactt gtatcagtat
     3901 aaatgatgaa atttgtcatg gattccctcg taaaaagcct ttgaaaaacg gtgatttggt
     3961 aaaagtcgat atgtgtatcg atttgaaagg cggcatttct gattcttgtt gggcctatgt
     4021 agttggagaa tctacacctg agatcgaccg attgatggaa gtaacgaaga aagctttgta
     4081 catcggtatt gagcaagcac aagtagggaa tcggattggt gacatcggtc atgctatcca
     4141 aacttatgta gaaggcgaaa atctagcgat cgttcgtgat tttattggtc atggcgttgg
     4201 tcctacgatt catgaagaac cagcagttcc tcattacgga gaagctggaa aagggttgcg
     4261 gttgaaagaa ggcatggtta tcacgatcga acctatggtt aataccggaa cttggaaaat
     4321 gaaaatggat cctaacggct ggactgcgta tacaagagac ggcggacttt cttgccaata
     4381 tgaacataca ttggctatta ctaaagatgg tccaagaatt ttgacatcac aaggagaaga
     4441 aggcacttat tgatctatcc agtcaatagg tacgggagag gtacatgaaa gtttggaaca
     4501 aaataaaaca aaatgaaaat ctgatgcgct ttattgagac gactcaaaag cgcatcactg
     4561 attcagaaat gagtacgacc tctgtcgtcg ttgcttatta tttactcttg tctttatttc
     4621 cattgattat tgcttttggt aatattttac cgtttctcca catcgatcaa gagactgtac
     4681 tgacctattt acgagaagtg attcctgaga ccgtttacca gtttatcggt cctgcaatca
     4741 aagacctttt gacacaaagt tcaggaggct tgttatccgt ttctgcatta gcggctttat
     4801 ggtctgcaag tcaaagtatc aatgctttgc aaattgcgat gaataaagcg tatggcgtag
     4861 aaaatagaaa aaattttttg atcgtccgtt tcttctcact aattgtcatt gtcttgttca
     4921 tgattgctat tagtggggta actatcgttc ttgggttggg acagctgatt ttggatgcta
     4981 ttcagccaat cgtacaaata cccgtgaact ttatcgatca gttccaaact ttgaagtggc
     5041 caatcacatt ggtagcttta tttgtcatca tgttcttgat ttatttaatt gtccctaatg
     5101 ctcagttgaa gttgaagacg atcattccgg gcacgatctt cgctactgtc ggctggatgc
     5161 tgttatcaca agtgtttagt atctatgcta ggtattttgc aacgaggatc agcggttatc
     5221 agatcatagg aagttttatt gtactcatgc tttggcttaa ttttgctgct acgatcatca
     5281 ttttgggcgg aatcatcaat gcagttgttc aagaatacgt gacgggtcat caaatcaaaa
     5341 aacgtcgaga tccaagccga aaatggttca ataaattgaa aactaaattt tctcataaaa
     5401 aaaagtagaa acaagagttc ttattgta
//
LOCUS       DAZUMM010000116         5344 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000116 DAZUMM010000000
VERSION     DAZUMM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.122"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            487..1086
                     /locus_tag="ACG8E6_002494"
     CDS             487..1086
                     /locus_tag="ACG8E6_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5062854.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1135..1851
                     /locus_tag="ACG8E6_002495"
     CDS             1135..1851
                     /locus_tag="ACG8E6_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFS5062855.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            1865..2644
                     /locus_tag="ACG8E6_002496"
     CDS             1865..2644
                     /locus_tag="ACG8E6_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFS5062856.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
     gene            2727..3083
                     /locus_tag="ACG8E6_002497"
     CDS             2727..3083
                     /locus_tag="ACG8E6_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5062857.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            3073..3339
                     /locus_tag="ACG8E6_002498"
     CDS             3073..3339
                     /locus_tag="ACG8E6_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5062858.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            3498..3776
                     /locus_tag="ACG8E6_002499"
     CDS             3498..3776
                     /locus_tag="ACG8E6_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062859.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(3773..4454)
                     /locus_tag="ACG8E6_002500"
     CDS             complement(join(3773..4420,4422..4454))
                     /locus_tag="ACG8E6_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS5062860.1"
                     /translation="MNHFKGKQFKKDVIIVAVSYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLDYLWKKKNRQSFYSWKMDETYIKIKGHWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQDHRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKEGTLFGFSVCTEI
                     RILLGIPA"
     gene            complement(4486..4647)
                     /locus_tag="ACG8E6_002501"
     CDS             complement(4486..4647)
                     /locus_tag="ACG8E6_002501"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFS5062861.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            complement(4971..5303)
                     /locus_tag="ACG8E6_002502"
     CDS             complement(4971..5303)
                     /locus_tag="ACG8E6_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062862.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
BASE COUNT         1863 a          858 c          954 g         1669 t
ORIGIN      
        1 tataaggtta attatgtatt atcacaaaat gattgttgtc gaataactaa aagtgtcaaa
       61 acaatgatga aatcatttag ttcttataaa ttagacaaga gaaaataaat attccatatc
      121 tactttattg gatccataac atgactaaat tctgaatact ttttaaagct tatcatttgg
      181 taaaactgaa tataagttaa ttatatagac tgaaaatttt gaattttcag aatagaataa
      241 cttaaaagta ctgacaaatt ttttaaaggg gcaatattaa tgttctgaga taatcaaatc
      301 ttaccgaaaa tatcaaaagt ccaatgtgac acaattggat tttgtcacat tcgttaaaat
      361 cgctccatcc ttagaggctc taataggaaa tcacttttct tttagcgcct tttttctata
      421 gcttgtttaa atgtgactta ttatctgtta taatcgtatt aagtaacatt tgagaggtgt
      481 ctaaaaatga aaagcgtgga accgattcgc gacaaaaaaa agatcgatgc catgaaggcc
      541 attttggctt ccggaaaata tgggcaacga aatttggtgc ttttttcgat cgggatcaat
      601 acagcctatc gaatctccga tttaagacag ttaaaattaa gggatgtgct agaaatttcc
      661 cgtgggcggg tgatcgtcaa agaacgtctg gccatgaagg aacaaaaaac ggccaaacac
      721 aattcggtct tcatttcgaa caaattgcga aaagtgattc ttgattatgt tcaatccgaa
      781 tttcctgagc agttgcaagc acaagacttc agcaagtatt tgtttccaag tcgaaaagga
      841 gcagatacgc ctttgacccg acaaagcctt tggcggatca ttcatgaagc aggaactgca
      901 gttgggttaa aagaaattgg tccccattcg atgcgtaaga cctttggcta ttttttatac
      961 aagcaaggaa ccaaaacaga gatcattcag tcgctgctaa accattcttc acaacgggag
     1021 accttacgct atattggaat cacccaggaa gacaaagata ctgcagtaaa gagtttggat
     1081 ttatgacaga ataatgccct atataataga aggaaaaatt caagaggtga gaaaatgaac
     1141 gaagaaaagc agcctaactt tcctgataaa tatcacttat ctcgcaaaga gtctgtgtat
     1201 ttattgaaga aaaacattgt agaactggtg tataatgcgg ggaaatttga agggttaaat
     1261 acaacgttgt tacaaacaga agggattatt aagtataatc gggccaacaa cgtggctgta
     1321 gatgatgtcc tgaccgtcgt taacttaaaa agaggctttg aaatgctttt aaatgacgta
     1381 caggagcccc tgttagagac aagtaagcgg attaatcgga tcgttgcagc agaggatgcg
     1441 ctttttcccg gtgaaatacg aaccggtggg gtagaattat cgacgattca aggaagatat
     1501 gtgccaccaa tgttaactga agacgaggta aacaatcaat atggtgagat aatgaatcaa
     1561 gagatatcag atacggaaaa agccctgcga ctgtttctat tcatttcgaa aaatcaaata
     1621 ttctggaatg gtaatgtggg tacagccttg ttgattgcga ataagattat gtttagcaac
     1681 ggtttaggac tattatcaat acccgaaaat gtatttatga aatttaacga acttttatca
     1741 aagtattgca acagtaatct attagtcaat gagtcaaata ttatatcatt tttatatgaa
     1801 cagtgtatct ttggtattaa ttatttgaaa ataatccaac atacaattta agaggtataa
     1861 gtaaatgatg actatagacg aagcaaaaca attagcaact tatttttcag ttggttcagc
     1921 gggatatatt ttagacttta ctaatgtaac gtggaacata tttacaaaga aaatcgtagg
     1981 gattgaaata caaaatcatt atgggggctc aaagggagta tcctttcaaa agtttctgct
     2041 tgacgatcaa tttgatgatt cgttaaagaa tcggttaatc caatctttaa aaggtattat
     2101 ggatcgagat aaaaaatatg aatctactga aatgaatccc atagttcagt tgctttttga
     2161 ggaagtattt aaagaagata cttcaactgc attaagaaaa atgaaatttt caagcgagat
     2221 tgatttactt tatattcggc agctacccat taggattcaa acagatatta aaaacaagga
     2281 ttttgactct gttttgacaa aagcaaatac tttaatagat gaagttctaa aatttattat
     2341 tgaaaatacc gaaggaccga gtgttaatat taatttattt aagtcaaaag aattaagaaa
     2401 agaagtattt ggccgcctca atattaaaac tgaaaaagat atggataata gaatcaaagc
     2461 ccttgttgga gcactcaata cattatcaga taaaatactc gaaatgagaa atagtcaagg
     2521 agatgctcat gcacatggct caaatagaat agtaattaag gaagaagaag caattcttgt
     2581 tgcaaattct tctatggttt tgtgcgagta tgtgtttact aagtataaag gattaaatag
     2641 ataataaaat gcatatttta tagtatacaa ttcgaatttt tatgttatat actataatat
     2701 aaaatcaata tatgatataa ttgggtatgg aaaaaatatc ttttgaaaca tttaaaagac
     2761 ccaatggtca tgatgaattt ttagaatggt tggaaacatt accaaaaaaa gatagtgcga
     2821 agcttttacg tacaatagaa gaaacagaaa agaatgggat gctagtagct caacggttga
     2881 aatgggtgaa aaaattagac actaatttgt acgaattaag aagtaaggta ggatcaaaca
     2941 tccaaagagc catctatttc catgtggaca atggaaggta tgtcattact cacggattta
     3001 ctaaaaaaac ggagaaaaca ccaataactg aactcaagca tgcaaaagaa cttagagagg
     3061 agtggttcac aaatgatgat taaaaaattg attgaagaaa agaaaaaaga taaagatttt
     3121 gcagaagcat atgaccgtga aggaagaaaa ctcgaaactg cggttgctct ttatcatgct
     3181 cgggaggaag cagggctaac tcaagcggag cttgcacaaa aagcacatac aactcaatct
     3241 actattgctc gaattgagag aggggataac gtatctttcg ataaacttga tgatatcgcc
     3301 agggcattag gtaaaaagct ggtagtagaa tttgcttaaa agcgagttga aaatatatct
     3361 ataataggct cctaactaat caattagttt agtgggcctt tttttactag atagataaag
     3421 gttgtactat agtgtaaaag acaataaatt ttatactaaa attactttaa agagaactag
     3481 agagaaaggt acaataaatg aaaaccataa atcttataaa agtatttata gcatcaccag
     3541 gtgatatagc agcaaaaagg aatgaagtag aagaaaccat atatagatgg aatatagaaa
     3601 atacagataa tacaaaagtg attttgatgc ctatacgatg ggaaactaat gcgattccta
     3661 cttatgtaaa taatagctct gggcaaagtg ttattaataa acaaattgta gaaaataggg
     3721 ttctgctgca aagttttaaa aataaaatca gtttttgtat tatttcttca ttttaagctg
     3781 gaattcccaa taatatcctg atttcagtgc agactgaaaa gccgaagaga gtgccttctt
     3841 ttcggctttt cttgtagatt cctcgaatgg tttccatccc tttaattgtg gtggaagctg
     3901 tacgtagact ttgatacaat ttatgtcgac gttttatcga tcgatgatct tgttctatta
     3961 aattgttaag atacttcaca gttcggtgct ctgtcttagt atataaaccc acactctgta
     4021 actttctaaa ggcggagcca agagaaggtg ctttatcggt cacaattgct ttcggctcac
     4081 caaactgttt atggagtcgt tttaagaaag cataggctgc ttgcgtatcc cgtttctttc
     4141 gtaaccagat atctaaggtt aagccgtccg catcaattgc acgataaaga taatgccaat
     4201 gtcccttaat tttgatatag gtttcgtcca ttttccatga atagaaggat tgtctatttt
     4261 tcttcttcca aagataatcg aggactttgc tgtactcttg cacccaacga taaatcgtag
     4321 tatgacaaac atttattcca cgatcatata acaattcctg aacttcacga tagcttagat
     4381 tgtaacgcag gtagtaacta acagcgacaa taatgacgtc attttttgaa ttgtttgcct
     4441 ttaaaatgat tcattactct atcctctctg tcttttttct caattttaca ctaaaataga
     4501 ttttttggaa aactttgcaa cagaaccact tttcgtaata tcaatgtctt tattcgtatg
     4561 atttttcgtt ttacgatcta catgattttg aagtccattt aacgacccaa ctttatgctt
     4621 ttgtgttcgt gccaccatat aggccataac tgtgtacttc ctttcgttaa aattattgcg
     4681 agaatcacaa gctaaattcg gaaaaacctc catgtaaggt ataacttact agacttatac
     4741 tgcgtaaagt ctgtaagctc tgccgagggc tatatcggct taacagccga tgagaaactc
     4801 cgtttctctc aaacgaaaaa gcgaacgaaa aacgactgcc ttcgcattcc ttttttacgt
     4861 ccttttttcg ttcaaaacca ttggcttttt gcgtcattgt tttcacaatt tttattccgc
     4921 aaaaaaccaa aagtgaaaag tattgctttt cacttcaacc cctcctcaat ttagttcaca
     4981 ttttctgaac gaaattgtgc cataaattga gtcaaaccct taacatcatt cgtacgccaa
     5041 tatgcaccat tttcttgcca aactaatcca tctgcaaatt ttcttagcga tactaaaacc
     5101 tcatcattta ctgaatgatt tcttttattt gattccaaaa aatctaatgc ttcagcaggt
     5161 gtcttaaagc cttccttttt acaatataat ttcgctaaag aaatcatttc tttctgtcta
     5221 atctgttttc gtttcgctct aatcttcgct tcttttgctt tcaaatccgc catttctttt
     5281 gccaatattt ctctattgtt cattttgatt cctcctaaaa ataaggcata tttctagttt
     5341 aaaa
//
LOCUS       DAZUMM010000117         5276 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000117 DAZUMM010000000
VERSION     DAZUMM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.123"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     misc_binding    complement(32..259)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(621..1823)
                     /locus_tag="ACG8E6_002503"
     CDS             complement(621..1823)
                     /locus_tag="ACG8E6_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5062863.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(1883..2086)
                     /locus_tag="ACG8E6_002504"
     CDS             complement(1883..2086)
                     /locus_tag="ACG8E6_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS5062864.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(2228..3292)
                     /locus_tag="ACG8E6_002505"
     CDS             complement(2228..3292)
                     /locus_tag="ACG8E6_002505"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5062865.1"
                     /translation="MDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFE
                     ELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQF
                     FRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNE
                     SNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKI
                     ISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSF
                     EKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQ
                     EEVEYYERKLQSISNEKTSY"
     gene            complement(3353..3460)
                     /locus_tag="ACG8E6_002506"
     CDS             complement(3353..3460)
                     /locus_tag="ACG8E6_002506"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5062866.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            3623..4435
                     /locus_tag="ACG8E6_002507"
     CDS             3623..4435
                     /locus_tag="ACG8E6_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5062867.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(4809..5000)
                     /locus_tag="ACG8E6_002508"
     CDS             complement(4809..5000)
                     /locus_tag="ACG8E6_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062868.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1630 a         1079 c          812 g         1755 t
ORIGIN      
        1 aaaaaaaaat ccttttacta gcaaatacta gtaaaaggac gattatactc gtggtaccac
       61 cttttttcag aaaaaacatt ctttctctca gcctgacaat aaaatcagtg gtcttggtaa
      121 cgaaagactg ttcgtttttg cctacctttc gacaaaaaat cctctaaggc cattttcgac
      181 agtcaacttc tagcttttta caccaaccaa agcctctctt gaaccatttt ctgtctactt
      241 tccttctcat cggatactat tagaataaaa ttagaatagc atgtttgaaa gaaaagtcaa
      301 tagggaataa gaacaaacaa aaactaattt gttattctta gtaatttgtt tgtggaatac
      361 aataaagctt ttctaatatg ataattgttc agtcgtcctt tcatcttaga tcacttcctt
      421 aaaatgatca tacccgtttc aaccacataa aaaaggactt aattttgaaa aatttcccat
      481 caaaactaag tcctttttat tatagttatg caaatttgct tattttctcg ctatttccga
      541 tacatcttga acaggtgcaa ttcgactgaa aacactggtg caaaagcatt tatttgtctt
      601 atggtagaac cgtggaaaac tcagactggt aataaattgt cattttttga aagaatagcg
      661 ccctcttgaa cattatcaat cgcacttacc acttgttgat ctgactttgc atataagtga
      721 ccgtaagttc ctaaagtgat ttgaatattc gcatggccca atcgttcttg aatagatagc
      781 ggttgaatat tctgctcaat tagaaatgca acatgagaat gtcgtagcgc gtgaagctta
      841 atcggtttga cacccgcttt tttagcagct tcttttaacc agttcgcaaa agaataaggt
      901 ggcgtaaatg ttccgtccaa ctgagcaaca tattcaaaat ttccaatacg tttttgcaag
      961 tcataccact gtctgagttt taaggaaagc ttcttcccca acccaatcgt tcgataagca
     1021 catttagttt tgggagtcga aaagtaccaa tcatcttttg tattataaat caatgtcttc
     1081 tcaatcgtta tcgtgttttt ctcaaaatct acattcgacc aagacaaagc ttcaagttct
     1141 ccaatccgaa tgccagtata aaagagcatt tcatacacaa tcttccgatg gtgggtttga
     1201 atcttatcat ccttcaattc attcatcact ttttgaaact cactgtgttc ccaaaactca
     1261 actttcttat tctcagaagg aagcgaagaa acatcttcaa cgggattttt agcaatcacc
     1321 ccctctttaa cagcccgatc caatacaatt tttaggtgcc ctcgaatccg attgatataa
     1381 gattttgata acgtttcgcc attaactttt ttccgcgaat tacgtgactc tgtctggctt
     1441 agttcaaata accaatcctc aatatcgtga cttgtaatat ctcgaacacg aaaatgatag
     1501 aaaaactgca tacgcttttc gaaaatgaat tgcgcattct catatgtttg acttttgacc
     1561 tgtctctcgt accattttac aaacctatcc ttgtaaaact cttcgaaagt catttgattg
     1621 gtagaacaca gttgattagg aattgtcttg ttaaaatcat gtgtttccat gtacttgtca
     1681 taagcaattt tggcatcttt ttgtgaagca taacctcgtt tagtcttctg aacctttctt
     1741 ccattttcat ctatacctag atacgctcgg aagaaccatt tttttgtctt tttatcttgg
     1801 taaatatttt taatttttgg catcgttcca ttcctccctg tacttgagaa aagctacttt
     1861 aatagtaccg cgcttgtact gtttattccg caacttgaat gtgaaaaaga tgttcaatta
     1921 ctcgggatgg caccacacca acttggcgat tattataaaa atcaatacct tcaatccttg
     1981 caagatatgc cttgctctct ttaatcattc gtgcagcttg actaggttta aagcccatct
     2041 ctatcaaaat tgcacgattt accatgaaat cacattttgt ttccattctc tcacctcctg
     2101 attctttact ccttttaaaa taatcctctt tataatctat tgtttctcat tcgatggttg
     2161 tgttatactc gtcttgtcga ggcggagtac gttgcacaac ttgttgtgta agcagtactt
     2221 tgctttttta gtagcttgtt ttctcatttg atatactttg taattttcgt tcataatact
     2281 caacctcctc ctgataatac tccgggtctg ccttcatatt ttggagaagt ttgatctgtc
     2341 ttggattcat ttctccttgt tcaataatca aaggcaacaa ggaagaatga aacgtttgat
     2401 cgacctgtgc aataaaaact aatgatggtg aaacaccatg aattaaccaa ctcatagttt
     2461 tctcaaaact ttgaggaatt gtatctaaag aaatggtgat tgcgccataa ttgctaataa
     2521 aggctgccca tttggcatca acttttgcac ctagatcatt tggatcacgg tcataaaaac
     2581 aaattgctcc attaagtaac tcaaagaaca gtttggttaa atcatgggtt tttgaaataa
     2641 tctttgccaa gctttgcgct ttctcttttc gataccgtag ttcaaagcga ttctttactt
     2701 ttgcctcttc taaaggaatt cctcgtcgct tccgttgttc atggtccttc tgataaaagc
     2761 aaaaacgaca ctgactcttc tttgagccta aatacaaggt ctcaccattt gattcattgt
     2821 ttatgatatc cgtaccgcca tgagattcac tcacttgaaa agtggtccac acatggccta
     2881 gtattacctt atgcttaagt tctggaatgc tgagactccc cacaaaatca tctaaagtaa
     2941 aatcaatccg tgtaaagttc ccctgataat ctaatacctt acgaaaaaac tgttgccaat
     3001 ttgtttttcg tgtttttagc catcttgcta agtgtttaca gccttgacct gaaaactcca
     3061 tcacagttcc tttaaccgca tcgtcaatgg aataccgaat cgtgattact tgattccaca
     3121 cgtattggcc aatatagccc tttggcgcat aatccagttc ctcaaaacgt tcaatgggca
     3181 tttcaataat ctcttcaatt agctttaaat gttgattcga gaaaaagtga atggttagat
     3241 aatcaatcaa aacacggtta actggaacga cctcttcttg caaactatcc aacacaagat
     3301 tcaatttttc ttcacttaat tgtctcgttc ctttttctac tttcgataat aactatctcg
     3361 aaatccccac tttttctgct aaatatcgtt gtgtccaccc cctttcttct cgagaaactc
     3421 gaatggcttc accgagatca tgttgattaa cttggttcat agacaaacct ccttctgcta
     3481 tttacttgtc aaagaaccta ttgaacttat tttaagtaca tttttattgt acgcctgtat
     3541 ttattttaag tcaatatgtt ttgacttttt ttaagtacac acgtatacta aatttaagaa
     3601 cttatcctga ggaggagttt tcatggagat agacaagcaa gcagtcggca atcgaattcg
     3661 gcagattcgc caagaattaa agttgagtat ggagaaattt ggcaaactaa ttggtgattt
     3721 accgcgaagt acagtcaata actgggaacg tgggatcaac cttccaaaaa ccgaaaccct
     3781 tcaccaaatc gcagagatag gtcatgtcac caatgaatat cttttgtatg gtgatcaaga
     3841 gaatcaatac attttagaaa tgctccaaaa aaaggcaggt aagcttgatc ctaagattga
     3901 aggtctcata gtagacgaaa tcaaacacgc tgaccttgtt agtgaaaaag aaatggatcg
     3961 aatgattgaa ttttttatta caaatctcat tccaccaact gaacaagacc agtttacttt
     4021 ccaatgtatc gatgaaaaaa agcagcttta tcttggttca actaattttg gtaaggaagc
     4081 ccagctctat ttgcaacatg ataatcaaaa tcatattctg cacatgatgc cctttacctt
     4141 ctccaacttt agtgtggatc gcatactcgt ctatttagca aatcaaaaat cctattccta
     4201 ttttgggaaa catttaccca aggaactagg tgagaaagcc atccttctat actcaattaa
     4261 tcaattgacc agcgatgtgc gaattgcccc gttggtctat tcagaagaaa ccgcttctta
     4321 tcagtacaca gaggacaatc aatatctact agaacagcaa tatctttatc taccattcgt
     4381 gatggaagtc gagaaagaac gcctgttaaa tgcagcgtat ccatcatcaa aatgaggtga
     4441 caaaattgat aagcacgtga caaagaaaat actgctagga cactccccaa tgggtagttg
     4501 tcacagtttc ctgttctttg ttagccccct gttagaaccg ggggctttat tcctgcagtc
     4561 cggctacgcc ttcctgcaat agacaaaact ccagatcggg taattcgctt gtggcgaaac
     4621 ccgacctgga gtttgtctct gtattttgaa ccctaaaaat taatcaaaca gactgatatt
     4681 ctcataatca gcgcgaaata agccattaag tacagttttc ctacctattt ttgataaccc
     4741 gctggttagt taatattcct tttccgagag agataaatga ttggttaaat ctatatacat
     4801 ctcaaccttt aaaaatgttt tcgaaatact tcatcagtac atgtcataca aattaccagt
     4861 gcattctcaa attctagttc tttaattgat ttcagttgag gagcagttac ttttggcgga
     4921 aatttccatt tttgtccaaa cctcttcccc cgatgaaatt tctttgttgc aattattgca
     4981 atgaaaaatt tttgtttcaa aaatatacat aattacttcc tccaataaaa actatctacc
     5041 tctcaaatta tttttctcta ataagaaaaa cagaaaaggt aataagatac aacagtgatg
     5101 attcctaata aagaaaagac tacaataata tttttctaac ttatcgcctt caaagaaata
     5161 gtttcttatt tttcactaaa ataaaaaatt tattgcaaaa aaaatagaag tgacaaaatt
     5221 atcaatcatt tcaaggtaat ataactacct gagtttatta tcgattaaaa tgctaa
//
LOCUS       DAZUMM010000118         5272 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000118 DAZUMM010000000
VERSION     DAZUMM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.124"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..1278
                     /locus_tag="ACG8E6_002509"
     CDS             43..1278
                     /locus_tag="ACG8E6_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFS5062869.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKPEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFAVLKRKINS
                     YQNPESHKTKLYCGAESFY"
     gene            1251..1331
                     /locus_tag="ACG8E6_002510"
     CDS             1251..1331
                     /locus_tag="ACG8E6_002510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062870.1"
                     /translation="MLWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(1457..2536)
                     /locus_tag="ACG8E6_002511"
     CDS             complement(1457..2536)
                     /locus_tag="ACG8E6_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5062871.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2639..3079)
                     /locus_tag="ACG8E6_002512"
     CDS             complement(2639..3079)
                     /locus_tag="ACG8E6_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5062872.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3136..3546)
                     /locus_tag="ACG8E6_002513"
     CDS             complement(3136..3546)
                     /locus_tag="ACG8E6_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS5062873.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3726..4160)
                     /locus_tag="ACG8E6_002514"
     CDS             complement(3726..4160)
                     /locus_tag="ACG8E6_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5062874.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4260..5108)
                     /locus_tag="ACG8E6_002515"
     CDS             complement(4260..5108)
                     /locus_tag="ACG8E6_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5062875.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1933 a         1015 c          878 g         1446 t
ORIGIN      
        1 tcttatttct tgcatacaaa aaaatcaagg agtgagagag ttatgagata ttcaaccatc
       61 ttaatatcaa catttgcagc cttaagctta tcgcttacta taaatcagac agtccacaca
      121 gaggagatag aaacagccaa atggacagaa accaagtggg ccaacgtcaa tcgaattacc
      181 tttcaggatg aattttcatc cttcaattca ggagtcgcaa aattaagaaa aggacaaaca
      241 gcgaccatca aagcaaaaat tgactattca ggagacttag cacctatact acaatcagct
      301 gttacatttg agaatgttga tcccgctttg tcgattggct atgacgagaa cactttgact
      361 atccaacaca agcaagcgat atttaccttc accgttacat taaatcagga cttgttaaaa
      421 ccagcgcttt ttactgtcaa ggtatcagat ggactaccga acgatcatca tcatttaacg
      481 ccgtatagtc agcggcagac aattgagcaa gattcagcta tcgactcagg aggagatctt
      541 gttgaagaac caacagataa accagaaaat gaaaataagc cagaagtacc gcctacagaa
      601 aatccagacg gagaacagaa accggaaata gaacctggag aagaacccga tacagaaact
      661 caaccagagc cagacaatga atcaaaacct gaaataaccc ctggagaaaa accagatgta
      721 gacccagaag aaaaaccaga tgtgactcca gaaccagata cggattctgg aaaccaaaca
      781 gtaccggaaa caaatccaga cacagataat gaaactgaaa atccagaaaa accagaggta
      841 gccccagaag aaaaaccaga tgtaactcca gaaccagata cggattctgg aaaccaaaca
      901 gtaccggaaa caaatccaga cacagataat gaaactgaaa atccagaaaa accagaggta
      961 gatccagaag aaaaaccaga tgtgactcca gaaccagata cggacgcaag agaccaagga
     1021 ataccagaaa aaataaacaa aaaaacaatt caagaggatg gaaaaaaaga gagtaaaaaa
     1081 tcaaatctag caattctaaa aataaatgaa gagcagctga ataaaaaatc acgaatattt
     1141 gatagcgccc aatcagctga aaccttaaaa agtagcaagg atacaacctt tgctgtcctg
     1201 aaacgaaaaa taaacagcta ccaaaatccg gagagtcaca aaacaaagtt atactgtgga
     1261 gcggaatcat tctattaagt atagcaacaa tgctttctgc aaaaagattt aaacaaaaca
     1321 gatctctcta acatttgaat aatgtctgtg aacgcatttt tcaaataaaa agaaatatcc
     1381 agaaaatgac cccaaaatac cagattgaaa tctgatattt ggggtcgttc atactttatc
     1441 tttcttcata tttattttaa gggatatctg tttcttttgg aagatggttc gggatctttt
     1501 tcactttcaa tacatctaaa aagaaggtaa agatcggaat gccgacaatc aagccccaaa
     1561 cgccaaagaa ccgttcgcta acaagtaaga ccacaaatgt ataaaaaatc ggcagttctg
     1621 ttttgctgga catcaacttc ggattcagca cataagactc aattagatgg acaatcaaaa
     1681 tcgtaatcag gatataaaca acatctcgga tgcctccttg ggaataagct atgaaagata
     1741 gaggaataca agaaatgata acacctgcta ctgggatcag acttaaaatg aaaatcatga
     1801 tcgctaaact cagcagctga tgaaagccaa atacagccaa ggcgattgta gtgagcaacg
     1861 tatttaaaag agcgacaaca aattgagctt ctaataccaa accaaatgta ttgacaaact
     1921 tatcggcaaa atagtaaata tcttgaaaga accaagaaaa ttcacttttt aaaaacaatc
     1981 ttgagaatac aattgttttt ttcttttcaa tcataaaaaa gaaacttaag atgaatgaca
     2041 tagcaaacgc cactgccatt tttcctatat cctgtaaata gccaagcgca atcgaagcgc
     2101 cattttgaag ctgttcaaaa aaatcgtttc gctcaatata agtatggata tattgtaaaa
     2161 caggatctgc atcttttggc tgatgctcgt aaaaattaat aacggactgg atcatgtcaa
     2221 atgtttgatg aataagcatc gggatatact ttgtgaatgc cagatagata aggtaaacga
     2281 tcagtgtata aatcatgata gctaaaacac ctgtagggat tcggacaaat cgctgaatga
     2341 tttgaacaag atgaatcatc aaataagtaa agataaatgt gagcaaaatg gttgtaatca
     2401 tacttctaac taagaaaaga acaaaaacga caaataataa aacagtgaga cggcgtattt
     2461 tttcattcgc caaaaaacgc tgataaacgg tcaaatcgac aacctccaaa taacaaaaaa
     2521 taaacacgtt attcattata tgatggaata tgaaaataag aaagagcaaa aagaaaaacc
     2581 gctaaatgac gttatcttat gagttggttt tcctcataaa atcgttcatc tagcagtttc
     2641 agcccttttt ctggtcttga tgaaaattat ctagcatttt tcgggtcaga tgttgcagtg
     2701 tatccaattc ttcctgttca agtggaagat aagacttgat cttttgtgga ataccttctg
     2761 cttgtttttg caacgcagca ccttgagcag tcagcgtaac atttacgatt ctttcatcac
     2821 ccgtttcacg ctgacgatta atataatttt tttctgctaa cttttttagt aagggagtca
     2881 gcgtaccaga atccagacac aaatcatttc ctagctcttt cactgaagtc tttttctttt
     2941 cccataaaac taacatcaca agatactggg tataggtaag attcagcggc ttgaggaggg
     3001 gagtgtagtg tctaatgatt tctttagagg cggcatataa aggaaaacat acttgattaa
     3061 ctaaatatag atcttccatt tttccacctc cgctgtttta ctatagtgta tcatagcgga
     3121 tttacacaaa aaagtctatt cactaactgc tttcagagta acttctatat ttccagctgt
     3181 agcttttgaa tacggacaca ctttatgcgc tgcctctaat aattcttgcg tttcttccag
     3241 agaaagacct tcgatagatc cttcaatctc tacacctaag acaaagctcg tttcactttc
     3301 tgaataaagg gaaacttgtg cactgacagt agaagcccca gagatatttt tttgctgttt
     3361 gatcaattct aatgcactgt taaaacatgc actgaaacca gctgcaaaca attgttcagg
     3421 gttggttgca ttttcgacgc gtttaccagg agctgcaata ttcaattcga atgtacgatc
     3481 gggattatgc acttctccag agcgtccgcc tgtgttgatc attttcgtag aatacatttt
     3541 tttcataaaa ttaccacctt taattatatt ttatacaatt gaattttaca caatataatt
     3601 gtggaaagca aagaaatgga ttggcataag ataaataaga gaaagcacat gaaacagacg
     3661 cgctttctcg aatagcaaac atatacaaaa aatatcagag acaaaaaata atctcatcat
     3721 aaacattaca tatatttctc aaaaagttgt tttctttctt caaaaattac ttcacgatgg
     3781 acctgatgaa gcatccataa ataaccaaaa ggatctctga acattgagtt agtcacacca
     3841 aagtccatca tttctgtcat ttcttgaatc tctacacagc catttcgtaa tgctttatta
     3901 tgtgtgtcct cgatatcaga gacgatgata ttgaaccaga tcgtatttgg atgttcagca
     3961 gtaggagcag tcaaaccaag agacgtgttt tcatccagca tatggaatct cgtaccatac
     4021 aaggtaaaaa tgacttcatt attgcctcta gggtaatctg ttgcctcaat cagttgtatt
     4081 tcatcaaaga ttgtttgata cagttctagt gctttcaaac tatcatttaa tacaaaatcg
     4141 atttccgttc ctttatgcat aaagatctcc tctccttttc agtcgaacag ttactagatt
     4201 cttttattat accagctaaa gaaaacgcat tcaaaaaaat agcggattta cttaccaact
     4261 cattcgattg gatgataagt aaatccgcgt cccattactt ctgctacgct gctgacaatg
     4321 acaaaggcac ttggatcgat ttcttctatc aatggtttca ttcgggaaaa ttcatgatca
     4381 gtcacaacga tcaataaaac ctcttttgac tttcgattat acccgccttt tgcctctagc
     4441 agggtaaggc cacgtccaat ctcagcactt aagcgaaggc ggatgtcttc caaataatgt
     4501 tcactcatga ccataacaga tttctttcga ttgatacctg tttcgatata agtcatgaca
     4561 attgaagtga ttgcaataga taaaatagca taaagaaact cttctattcc aaaaataaac
     4621 agattaagag aaacaacgaa tgcgtctgta aaaaataagc caatcgaagt attcaagtgg
     4681 aaatattttt taaaaatgag gggagggatc gtcgtaccgc cgctagaaga gttgttttta
     4741 tataaaatcg caactcctaa agcaaaaatc gcacttccaa aaatgacaga caacaaccga
     4801 tctgaagtaa tcattgtttc tggaactatt gcaatgatta aaggaaaaac caagcttccg
     4861 aaaagtactt taaaaaaggg ttttttacct aaaaatagaa gagctagtac caacataacg
     4921 atattcaaca ctaaaataac tatagctcgg tcaaagccta aagcagcttc cagtaatatt
     4981 cctagaccgc tgacacctcc agcagcaata tgatgaggcc ctaaaaacat attgatagcg
     5041 actcctaata tgaagagggc aaaaataatc ttaatgtatg tccatataaa ttgtctcgtt
     5101 tctttcattt attcacttcc ttaattcatt aataatactc ttatcttatt ataacttact
     5161 tttcacctta agttaacttt aattaataaa aaaattaggc tagtctaaat tattatgttt
     5221 cattcttcta gaatatactt ctacgccaaa aactgttaaa aaacttttac tt
//
LOCUS       DAZUMM010000119         5141 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000119 DAZUMM010000000
VERSION     DAZUMM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.125"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..331
                     /locus_tag="ACG8E6_002516"
     CDS             <1..331
                     /locus_tag="ACG8E6_002516"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062876.1"
                     /translation="LENGLVFEDQYLQIKNSENIEACIEISNSQESNIFVSPENGPVK
                     PNFNYLTYDRFSQNTVFDGYKLEQSSPAIHSGKKVIDKNGYNLGTDFFGIKLDGILDI
                     GAVKSSK"
     gene            353..1165
                     /locus_tag="ACG8E6_002517"
     CDS             353..1165
                     /locus_tag="ACG8E6_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5062877.1"
                     /translation="MNDISFFQIFSLICLAILGGIDPYLSGLQLSKPAISGFLAGIIM
                     GDINTGLLVGATLQLMVLGVGTFGGSSIPDFGTGAIIGTALGVVSGKGIEFAIGISVP
                     VGLLLVQLDILARFCGVYFLHRVDKHIEEENFHKISLEAWLSLVPIALSRAIPVGLCL
                     IFGNEVVNTVLNYAPDWLMGGLKLAGAILPVVGIAILLHYLPIRNFFGFLIIGYILAA
                     YLNIPMMGISFAGLAAAIIHYKKLQDKRLLESKLDTINHIEVVNGEVDDDEI"
     gene            1152..1967
                     /locus_tag="ACG8E6_002518"
     CDS             1152..1967
                     /locus_tag="ACG8E6_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5062878.1"
                     /translation="MMKSNVSKLSSKDLRKVSLRYMFGAQLGWNYERMMNVAYVRAVY
                     PALKKLYKDEKELKSVLQSEMQFYNTSPFLSAFIIGIDLALQVEDGSKSKDAVAAIKT
                     SMMGPFAAVGDSLFGAVIPTIFGSLAAYMGLEGNPLGVLLWLLVSLIILFLRYFELPI
                     AFREGKKLVSSVGGLLKDLTESATLLGVVVIGGLVPSVVKVGVPFKLVIGDKKLSFQT
                     EMLDPIMPAIVPVVLVLLAYWLLSKKSLNSTKVIWIFLVLSILMYSLNILAVL"
     gene            2286..3743
                     /locus_tag="ACG8E6_002519"
     CDS             2286..3743
                     /locus_tag="ACG8E6_002519"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HFS5062879.1"
                     /translation="MKKNQPNVVLIVVDQMRADALSLNSQDKIISTPTLDMMASQGYN
                     FENCYSPVPSCVPARAALLTGLDQETSGRVGYEDEVPWNFKNTLPEVFKEQGYQTECI
                     GKMHVYPSRKRLGFDHVLLHDGYLHVDRKYDKSYGEQFEYSSDYLMFLKESLGSDADL
                     IDDGLNCNSWEARPWMYPEKFHPTNWVVSEGINFLRRKDPTVPFFLKLSFEKPHAPLN
                     PPKYYFDMYMDRLPDTLDLHIGNWEKLEHVVPDVCALRGKLKEDDQRRMLAGYYGLIS
                     HIDHQINRFLMALKEFRHDKDTIIWFISDHGDQLGEHYLFRKGYPYQGSIRIPSFIYD
                     PGDLISAKKHGIKELVKIQDIFPSLVDLVLGQYVNTDGKSVKQLLFGNCEGWRREIHG
                     EHSLGLDSSQYILTEKWKFIWFPVKNTYQLFDMINDPNEMKNLYYDKKYESIIYEMKH
                     KLVGYLKGREEGFVKNGQLIQIGISNIVSTLKSKK"
     gene            3934..4100
                     /locus_tag="ACG8E6_002520"
                     /pseudo
     CDS             3934..4100
                     /locus_tag="ACG8E6_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(4902..>5141)
                     /locus_tag="ACG8E6_002521"
     CDS             complement(4902..>5141)
                     /locus_tag="ACG8E6_002521"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062880.1"
                     /translation="IKKKFKQQFKKILTAEIDVAISQSSTKYDPAFQKLIDKCLFIIK
                     IKEKFMSAFFIKVVMIIRLLIVFFRKYGIIVLTKR"
BASE COUNT         1756 a          631 c          965 g         1789 t
ORIGIN      
        1 attagaaaat ggattggttt ttgaagatca gtatttacaa ataaaaaatt cagaaaatat
       61 agaggcgtgt attgaaatca gtaatagcca agagtcgaat atatttgttt ctcctgaaaa
      121 tggtcccgta aaacctaatt ttaactattt gacatatgat cgattcagtc agaatacagt
      181 gtttgatgga tataaattgg aacaatcatc tcctgctatt catagtggga agaaggttat
      241 cgataaaaat ggttataatt taggaactga tttttttggt attaagttag atggcatact
      301 agatattggg gcagtgaaaa gttcaaaata atataaagga gttagtttga tcatgaatga
      361 tatttctttt tttcagatat tttctttaat atgtttagct atactaggag gtatagatcc
      421 ttatctgtca ggattacaat taagtaaacc ggcaatttct ggttttctag caggaattat
      481 aatgggagat attaatacag gcttattagt aggagcaaca ttacaactga tggtcttagg
      541 tgtaggaaca tttggtggct catcaattcc tgattttggc acaggggcaa tcattggtac
      601 agcattaggt gttgttagtg gaaaaggaat tgaatttgca ataggtattt ctgttcctgt
      661 aggcttatta ttagttcaat tagatatttt agctcgtttt tgtggggttt attttttaca
      721 tcgtgtggat aaacatatag aagaagaaaa ttttcataag attagtttag aagcatggtt
      781 aagtttagtt cctattgcat tatctcgtgc aataccagta ggtttatgtt taatttttgg
      841 taatgaagta gtaaatacag tattaaatta tgcaccggat tggttaatgg gaggattaaa
      901 attggcagga gctatattac cagttgttgg gattgccatt ttactacatt atttgccaat
      961 taggaatttt ttcggttttt taattattgg gtatatttta gccgcttatt taaatattcc
     1021 aatgatgggt atttcttttg caggattggc agctgctatt attcactata aaaaattaca
     1081 agataaacgt ttattagaat caaaactaga tactatcaat catatagaag tagttaacgg
     1141 agaggttgat gatgatgaaa tctaatgtta gcaaattaag tagcaaagat ctgagaaaag
     1201 ttagtttaag atatatgttt ggagctcaac taggttggaa ctacgaacga atgatgaatg
     1261 tggcatatgt tcgagctgta tatccagctt tgaaaaagtt atacaaagat gaaaaagaat
     1321 taaaaagtgt gttgcaatca gaaatgcaat tctataatac cagtcctttt ttaagtgcat
     1381 ttattattgg aattgattta gcattgcaag tagaagatgg aagcaaatcg aaagatgctg
     1441 ttgcagcaat taaaacgagt atgatgggac cttttgcagc tgtaggcgat tctttatttg
     1501 gggcagtgat tccaacaatt tttggatctc tagcagctta tatgggatta gaaggaaatc
     1561 cattaggtgt actactttgg ttattagtat ctctcattat actatttttg agatattttg
     1621 agttaccaat tgctttccga gaaggtaaaa aactggtatc cagtgtaggt ggattgttga
     1681 aggatttaac tgaatcagca actttacttg gcgtagtagt aataggaggc ttggttccta
     1741 gtgttgtaaa ggttggtgtt ccattcaagt tagttattgg tgataagaaa ctatctttcc
     1801 aaacagaaat gttagaccct attatgccag ctatagtacc tgttgtttta gttttattgg
     1861 catattggtt attaagcaaa aaatctctca attcaaccaa agttatttgg atatttttag
     1921 ttttgtcaat actaatgtat tcattaaata ttttagcagt tttgtaatag atgtgggggc
     1981 gtgatcaact aatttatggc atgtcccctg tttaattttc taaatattat atttttaaat
     2041 gagtggaaag aagatattag agatttaatg aataataagt tatatagata aaaaattaaa
     2101 gatattttga tatcaaggtt tattttagta taaagtattc gtgactaatc aatcatacgc
     2161 tataaagttt ggtaatccat ttatatttct tgttatcaca aaaaatatat tctttcaaaa
     2221 ggaaatatca atttcttaaa tcaagagaga ttatttggat attttgaatg attggagttt
     2281 ttaatatgaa aaagaatcaa cccaatgttg ttttaattgt agtagatcag atgagagcag
     2341 atgcgttatc tttgaatagc caagataaaa tcattagtac gcctacatta gatatgatgg
     2401 ctagtcaagg atataatttt gagaattgtt attcgcctgt tccaagttgt gttccagcaa
     2461 gagcagcgtt attaacagga ttagatcaag aaacttcggg aagagtgggt tatgaagatg
     2521 aggttccatg gaattttaaa aatactcttc cagaagtttt taaagaacag ggatatcaaa
     2581 ctgagtgtat tgggaaaatg catgtgtatc cttcaagaaa acggttggga tttgatcatg
     2641 tactattgca tgacgggtat cttcatgttg atcgaaaata tgacaaatct tatggagagc
     2701 agtttgaata ttctagtgat tacttaatgt ttctaaaaga atcattgggc tcagatgcag
     2761 atttgattga tgatgggtta aattgtaatt catgggaagc acgaccatgg atgtatcctg
     2821 aaaaatttca tccaacaaat tgggtggttt cagaaggaat aaacttttta aggcggaaag
     2881 atcctacagt accatttttc ttaaaattat catttgagaa acctcatgct cctttgaatc
     2941 caccaaaata ttattttgat atgtatatgg atagattacc agacactcta gatttgcata
     3001 ttggtaattg ggagaaatta gaacatgttg ttccagatgt ttgtgcctta agaggaaaat
     3061 taaaagagga tgatcaaaga agaatgcttg ctggatacta tggccttatt tctcatatag
     3121 atcatcagat caatcgcttt ttaatggctt taaaagaatt tagacatgat aaagatacaa
     3181 tcatttggtt catttcagat catggagatc aattaggaga acattactta ttcagaaaag
     3241 ggtatcctta ccaagggagt attcgtatcc cgtcgtttat ttacgatcct ggtgatctga
     3301 tctcagcaaa aaaacatgga attaaagagc ttgtaaaaat acaggatata tttccttcat
     3361 tagttgattt agtattagga caatatgtaa atacagatgg aaaaagtgtt aaacagttgc
     3421 tctttggaaa ctgtgaagga tggcggcgag aaattcatgg agaacatagt ttagggctgg
     3481 attctagtca atatatatta acggagaaat ggaaatttat atggtttccc gtaaaaaata
     3541 cgtatcaatt atttgatatg ataaatgatc caaatgagat gaaaaaccta tattatgata
     3601 aaaaatacga atctattatt tatgaaatga aacataaatt agtaggatat ttaaaaggta
     3661 gagaagaggg gtttgtcaaa aacggacagt taattcaaat aggaatttca aatatcgttt
     3721 caactttaaa aagtaaaaaa taaactaaaa gtgaagatgc aataaaaaat caacataggg
     3781 tatactgagt caaaatatac tcagatacag gcaaaacttt gcgtctttat ttatttcaaa
     3841 aaaatcaaat attcggatgg taacaaacat actactttgt ttgggataaa atcagatctg
     3901 agtcagagta agttcattct gattcagttt tttatgtcta acgacgataa aagtttagca
     3961 catacaagat ggaattgtaa gtatcccata gtctttaccc cggagtatcg atgaaaagtg
     4021 atttatggaa aattaagagt aagtaactgt tggtgatagt atcaccccaa attagtattt
     4081 ccagttttat gagctattta ttaaataaat tctatacggg atttgaagat cctgaaggtg
     4141 aaatcgtcat ttatcagaat aaataaatca aatagtaaaa gttcaaagaa tgaggggaac
     4201 attagttttt attgcttagt ttaatcccag taacgatttc tgcaagatgt aaaaggtctt
     4261 tcatttcata agttttcaga agcaagttca tacgcactaa ctcactttcg attgagtagc
     4321 aatcgtcaac aaactttact atcagctgtt atttttattc tttcgcgtta tctaaatacc
     4381 atcaatttat aagttgtagg tgtatttaat aaaagaacaa ataactttag tatttttatt
     4441 aatctaactt acaaactagt agtatagggt atagtaaaaa tatgataaaa taaatagtcg
     4501 ttatcttaat ccaactatat agaaaattta cgttttctat atagttggat gtttacaagt
     4561 tatatctggt ttaataagtt tttttctgtt agtcatggca tgttttattg tgctggttca
     4621 tcaatatatt gaaatgtact aaaatgaaat tttcagttat ggtataatat ttatgctgtc
     4681 tgctaccccg gcctattatg gcaaggggag gaggttgtat tatgaattac cttttcgata
     4741 tgttttttgc aatcttagca gaagtatccg cgcattacat ctgtaaatgg cttgattgca
     4801 aaattattgc caaaaaagac agtaagccta cccgttaatc tgaatagaac gtaaaagaaa
     4861 accactagcc cgccagctag tggtttttgg tgtatcatga attacctttt cgttaaaaca
     4921 attataccat atttcctaaa aaatacaatt aatagacgaa ttatcattac cactttgatg
     4981 aaaaaagctg acataaattt ttcttttatt tttataatga ataaacattt atcaataagc
     5041 ttttgaaagg caggatcgta ctttgttgat gattgagata ttgctacatc aatctcagca
     5101 gttaagattt ttttgaattg ttgcttgaat tttttcttta t
//
LOCUS       DAZUMM010000120         4948 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000120 DAZUMM010000000
VERSION     DAZUMM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.126"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            53..739
                     /locus_tag="ACG8E6_002522"
     CDS             53..739
                     /locus_tag="ACG8E6_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFS5062881.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            741..1685
                     /locus_tag="ACG8E6_002523"
     CDS             741..1685
                     /locus_tag="ACG8E6_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFS5062882.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1884..2528
                     /gene="pcp"
                     /locus_tag="ACG8E6_002524"
     CDS             1884..2528
                     /gene="pcp"
                     /locus_tag="ACG8E6_002524"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFS5062883.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2607..3884
                     /locus_tag="ACG8E6_002525"
     CDS             2607..3884
                     /locus_tag="ACG8E6_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062884.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(4068..4817)
                     /gene="pnuC"
                     /locus_tag="ACG8E6_002526"
     CDS             complement(4068..4817)
                     /gene="pnuC"
                     /locus_tag="ACG8E6_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFS5062885.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         1592 a          868 c         1060 g         1428 t
ORIGIN      
        1 tgtgttttgc tttataatta aaaaaatatt gtcattttat ggaggaaaat atatggaaaa
       61 ttatcttagc cttttaggcg tcttgattgt tattctcggg tttgcattta aattagactc
      121 gatattgatc gtaatggttg ctttagttgt tacagctttg actggcggtc ttggcattca
      181 aggaatgtta gaacttttag gtacaagttt tgtaaacaat cgcgggatgg cgatttttat
      241 catcattatg ttggcaacag gaacgcttga aagaaatggt ctgaaagaat cagcggcttc
      301 cttgatcaag cgattcaaga aagtatctgc tggtatgatt gtagatattt atgggatctt
      361 tcgaatgatc tttgcggctt ttaacgtgag ttttggtgga gttgctggat ttgttcgtcc
      421 aatcatttta ccgatggcat tggggaccat cgaatcaaaa gggttagaag tccatcctaa
      481 gcatgaagaa gaactaaaag gtatggcttc agctatggaa aatgtctgct ggttctttgg
      541 ccaagtacta ttcatcggag gagcaggggc gttgctagtc caatcaacac tgaaagatct
      601 aggatatgac gtaacattgg gacagcttgc tttagtggaa gttccagtgg ctatcacggc
      661 tttagttgtg gcaagtgtct acttctatct taaagaaaag aaattagcga agaaatatta
      721 tccaggaaag gggcaataag atggactttt ttacaaatcc tgatatttta ttaggcgata
      781 aattactgga gatcctttat atcgtgatgg gactcgtatt gatttacact ggagtaagaa
      841 atttaacaga taaaaccaat cctcatcgtt atgggtcagc ctttttctgg acggtcttag
      901 gggtagttat tgcaggagga agatggcttc cggcaatcgt gaacggtggc ttgatctttg
      961 cgatgaccat accagcaatt gctaagaaag tcagcaaagg agaaagccgg cttccatcaa
     1021 aagaatatat ggaaaaaatg tcagacaaac taggaatgaa aatttttatt ccagctttga
     1081 gtatcggtgt ctttgcgatc ttgtttgctc tatttaccaa tttaggtgct ttagtcggcg
     1141 tgggcgtcgg tgtttttgca gccatgctaa tcttgatgtt cttttctcga gataatcgac
     1201 caagtacatt tttagatgat gcagcagata tgctgggcac agtagggccg ttaagcatgt
     1261 tgccaatgct tttagcttct ctaggtgcgg tatttacaag cgctggagtt gggacagtga
     1321 tttccaatgc agtaggtacg atcgttccag aaggaaatgt gaacatcggc atcatcattt
     1381 acgctcttgg catggtgata tttacggtaa tcatggggaa tgcatttgcg gccatcaccg
     1441 tgatgacggt tgggatcggt gcaccattcg tatttagtca tggagcgaat ccagcgttaa
     1501 taggaatggt tgcattgact tgcggttttt gtgggaccct gctgacaccg atggctgcta
     1561 acttcaatat cgtgcctgta gcgatgctgg agatgaaaga caaatacgga gtgatcaaaa
     1621 accagttgtt tattgcgtta tttatgctag ttttccaaat tgcttatatg attctattta
     1681 aataatagaa gagaggggtt gtgacagaat aattgtcgca acctcttcta acattgtagt
     1741 ggaacactat ttttttataa gaatatatta agtttgatct gttatgttat ttaaactatt
     1801 gtttccttta tttacgctgt tcaaggaaaa tggtatgata aaagataacc atatgttaaa
     1861 tcaatcggac ggaggaacaa cagatgaaag tactagtaac aggttttgat ccatttggtg
     1921 gggacaaagt gaatccagcc tatgaagcag taaaaaaaat gccagatgaa atcagcggtg
     1981 ctgaaatcat caaagtggaa gtaccgaccg tattcgaaaa aagcagtcaa gtagtcaaag
     2041 aagccatcca acagcaccaa ccagatgtcg tgatctgtgt cggacaagca ggtgggcggt
     2101 cagcagtctc gttcgaacgt gtcgcaatca atctagcaga agcaagaatc cctgataatg
     2161 aaggaaatca gccattcgat acggctttag aagaaaacgg tccagccgct tactttacct
     2221 cattgccaat caaagcaatg acaaaaaatg tacaagatca tggtctacca gcgtacatct
     2281 cttatacagc aggaacattt gtctgcaatg atatcatgta tcgtttgctt catttgatcg
     2341 aaacacaatt cccggcaatt cgaggtgggt tcatccacgt gccattctca cccgatcaag
     2401 tgatcgatcg tccagtgggt acgccttcta tgtctttaga agacattgct tcttctttga
     2461 cttatgcagt tgaagcagca gtagaaaata aagaagatat ttccggaaat gccggaacaa
     2521 ctcactaatt tagcaagaaa cactggctgg ttgctaacgt tatgttagta gccagctttt
     2581 tgtaattaga aaggaaagaa acagccgtgg aagaatggca atccgtcttt gaagaatggt
     2641 ttccaaaaga aatcagcaaa agctatccca tcaaaatttc taaacaatac acaagcagtc
     2701 aacgttggga gatctatgca aaattaacga aaaaacagcg ggaactagtc gataagcacc
     2761 gccgatatct aatcagctct cgttttatgg aagaacatta tttagctgca acagattggg
     2821 tgttttctga cttcaagatc aatccgtttt ttcgaacaaa acgaagccag caaaaattgt
     2881 attgtgagtg tggtagagaa ttaaaagtgc aatacatcgt taaatcgcca aaaaccggaa
     2941 agatcctaaa actaggcatc aaccactttg ccgatcacct gcatgtctct ccgacagtgg
     3001 cagcttcgat ccatcaggga atgacaaaag ttgatctggc cttagacgaa ttattatggc
     3061 ttaagcagaa gaatatcgat tttcctgaag gactatggca aaaatactgt tttgtcttgt
     3121 accaaaatcg tcggatgaaa cagccatatt taccggatat aaaattagct cagcggctcg
     3181 cagaatttcg ccaagtagaa atgccgattt acatagcgga ttatcaagca ttggaaaatg
     3241 aaatcaagaa aataagtgaa cacatcaatg ggcagtcaaa aaagcgacag ataaaaaaag
     3301 aactatttga cgactttgca gaagaattag taaaggatgt agaagaattc ttgatcaact
     3361 atcgcgcatt tttacgtaaa gattggcaat ccatcgtata tgaagaagtg ccagtacatc
     3421 ctaatgctta ttttgaaaca tttatttccg tgttgagaaa gacaaaacgt caacgaacac
     3481 cagaagtcac tgctcagatg gaatattttg cgaaaaacca acgattcatc caaccaaaaa
     3541 tctatctatt tatttggaag caatattgtc gctatggttt tacagaaggg ttcttcgaca
     3601 gcattcctcg gatagtcaga aatggttttt taaaagtatt gcgtaaagaa cgagaagcta
     3661 tacaatctgc tgataagaaa gaccgtacag tctcaaaaga aaaatggcaa ctagtagtga
     3721 aagatatcca atctggaaat gtgcaagaga caatcgacaa atggaaagga aaacattatc
     3781 gttttactga agctcagaaa caagcgttgg aatattatca gaagttagaa gaatcactgc
     3841 gtttcaatga tgaggcaaga aagtatctga aggaattact ataaaaaata gaggctggga
     3901 cagaagcgtt taactccaag aaataagaag aaattcacga aaattgcttt tcaaattttt
     3961 gtgaatttca gcttatttcc gaaggagttg cttctgattc cgccgtttat acgtgtttag
     4021 agcgtgagac aaaagtgttc tttacttttg tcccacgctc agttttttta tgcgtgttgt
     4081 tgtgcgttgt agtattcttc ttccattttt tgcatacgtt cacgttcttt ttggttatac
     4141 caaggtgaaa cggtaaatgc aaggacatca ttcagcaagt aaacagagct gttcacgaac
     4201 atcgccaaac ttgcagatcc atgcgtaaat gaaatatacc aaaggaccat ttgagcaagc
     4261 cctgaagcta accaccagta atattggttg ttgtaacgca agaaactgat gataccagca
     4321 gtcaagctaa tagaaaaagc aatggcatct acccacggcc gaggatcatc tgtataacga
     4381 ccaatcagga atcctgaaac aagatagaca aggaaagtac tgatgattgc gataaaccag
     4441 ctttttccag tgaattttcg gatttttgaa gccatattat gattccattc tttacttaac
     4501 aatacgggga tatccaatgt gatcatataa gcaatctgtt caccgatact taaatagttc
     4561 tttgctgaaa acccaacgat aataaaacaa atcgcagaca aaatacctaa aataccgttg
     4621 actgattttg ccgcattgat ggataaaata cacaatacac caagcgtggt accgataaat
     4681 gtaataacgg acagccaagt gatgggacta ttgacaagag tcatgacctg gcagccaaga
     4741 ctgaaaaaga aaagataata attttgttgc ggccagcctt ttagctgatg agctaaaaac
     4801 tgaaaatatg acttcatgta tgaggactcc ttaagacact aaatgtagtg tgttctgtat
     4861 ttactaacac aatctattgt atgcttttct tgaaaaaaaa ctagtctttc aattataaat
     4921 aatttgtgtt atgataattt tctgtttg
//
LOCUS       DAZUMM010000121         4931 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000121 DAZUMM010000000
VERSION     DAZUMM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.127"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACG8E6_002527"
     CDS             85..972
                     /locus_tag="ACG8E6_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5062886.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8E6_002528"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8E6_002528"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFS5062887.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACG8E6_002529"
     CDS             2274..2720
                     /locus_tag="ACG8E6_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS5062888.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACG8E6_002530"
     CDS             2993..4345
                     /locus_tag="ACG8E6_002530"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFS5062889.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACG8E6_002531"
     CDS             4516..4803
                     /locus_tag="ACG8E6_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS5062890.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1393 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt t
//
LOCUS       DAZUMM010000122         4893 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000122 DAZUMM010000000
VERSION     DAZUMM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.128"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(494..1246)
                     /locus_tag="ACG8E6_002532"
     CDS             complement(494..1246)
                     /locus_tag="ACG8E6_002532"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFS5062891.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1359..2303
                     /locus_tag="ACG8E6_002533"
     CDS             1359..2303
                     /locus_tag="ACG8E6_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5062892.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQLCTRLARLY"
     gene            2352..3287
                     /gene="murB"
                     /locus_tag="ACG8E6_002534"
     CDS             2352..3287
                     /gene="murB"
                     /locus_tag="ACG8E6_002534"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFS5062893.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAAFAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3585..>4893
                     /locus_tag="ACG8E6_002535"
     CDS             3585..>4893
                     /locus_tag="ACG8E6_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5062894.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVI"
BASE COUNT         1699 a          800 c          946 g         1448 t
ORIGIN      
        1 tctcaataaa cggtgttcaa aagcttgcat ctgcggtaat aagtccaacc aaacgaaaaa
       61 tatgaggaac tatttttgtt tggctgttct tattacttga tgcagtgcag ctttttcaca
      121 acctcttaat aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa
      181 aaatatgagg aactattttt gtttggctgt tcttattact tgatgcagtg cagctttttc
      241 acaacctctt aataaacggt gttcaaaagc ttgtatctgc ggtaataagc ccaaccaaac
      301 gaaaaatatg aggaactatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
      361 ttcacaatct ctcgataaac agtgttcaaa agccagtttc tcggtattat gccaaattta
      421 tgcaaaatgt ggaaagccat tttcacaaaa tttttcttaa tactcgaaac taaacgactt
      481 tttcacaacc tctttatttc aaatctaatt ctactggaca atgatcactg cccataatgt
      541 ccgtatggat ctttgcatca gctagtcctg gctctaaatc tttgctcgtc aaaaagtaat
      601 cgatccgcca accagcattg tttttccttg aattgaatcg gtagttccac caagagtaga
      661 caccttcctg ggttggataa aaataacgga atgtatcgat aaacccgtta tccaacagtc
      721 gagaaagagc cgctctttct tctggagtga atccagcatt tttttgattc gttttccagt
      781 ttttcaaatc aatcttttga tgagcgacat ttagatcccc acacacgatg actggttttt
      841 gtttttttag attttccaaa taattataaa aggcttcttc ccattctaat cggtaatcta
      901 aacgttttaa ttcgctttga gaatttggcg tgtaacaggt tacaagaaag aaatcggaat
      961 attccaaagt aatcagacgg ccttctgtat catggatatc gatacccatt ccgtaagtgg
     1021 cattcaatgc tggtttttta gcaaaaatcg ctgtgccgga atagcctttt cgttccgcgt
     1081 aattccaata ttgataataa cctggaagat ctaaatcaat ttgtcctgct tggagtttcg
     1141 tttcctgcag acagaaaaaa tcggcgtcta actcatgaaa aacttctaaa aaatttttat
     1201 ttacgatggc acgaagcccg tttacgttcc aagagataaa tttcaataaa agcacactcc
     1261 ttcgaattaa tttccttacc tttgcgatac tattgtacct cattttagta aaaaatggtg
     1321 taagatgaaa gtaactaaat gatccaggag gaaagtagat gaagattggt gtaatcggtg
     1381 tagggaatat cgcagaaaaa gcatatttac caacttatgc gaaaaaacaa ggaacacttg
     1441 atttttattt tgcaacaaga aataaagaaa caaaagaaag aattcaaaaa gagtacgggt
     1501 tccaacatct atatgaaacg atcgatgagc ttttagaaga aaaaatcgaa gcatgtatga
     1561 tccacgcagc aacaaaagta cattatgaat tagccaaaaa atgtctagaa aatggtatcc
     1621 atgtttatat tgacaaacca ctgagtgtcc atctaaatga agttcgtgaa ttgcaagaat
     1681 tagctgataa gaaccaaata attttgatga tcggtttcaa ccggcgcttt gctcctatgg
     1741 tagaagaatt gaaacgtatc ccagataagc ggttgatcca cctgcaaaaa aatcgaatag
     1801 cggcaaaaga aacgacagag ttcgtccttt atgatttgtt tttacatctt attgatacag
     1861 ctgtgtattt attagatgaa cccattttga gaacaaaata ccagataaga gaaacaaagg
     1921 aatttttgga atatgccatt ttacaattag aaacacaaaa tcaaacggct attttaacga
     1981 tggatttagc aagtggcgct aatatagaaa aatatcaagt aacaagtaaa aatggtactt
     2041 atgaagtaaa cgatctgacc cagcttgtca tccaagatgc tgatggaaaa aaagtgaaag
     2101 aatttggcga ctggacaaac acgctagtca aacgcgggtt tgagccaatg gtagaggcct
     2161 tcttttctca gatccgttta ggtaaatcgg aaacagaatt gcttaaacaa aaagaagttg
     2221 taagaagtca tgaattatgt gaggaagtgc ttaaagatca ctaccgtcat caactttgta
     2281 caagactggc aagattgtat taagcgtgat agaataatga ggacgtttga gaaaagaaag
     2341 gaaaatcctc agtgaataaa gaagaaatag ttgaaagctt agccacgatt actatattgt
     2401 ttgatgaacc attgatgaac tatacgttta cgaaaacagg tggaccagct gatgtccttg
     2461 cattcccgaa aaaacaagaa gaagtaaagc aaatcatcga ttattgccga atccatgata
     2521 taccttggat ggtattagga aatgcaagca atctgatcgt tcaagatggc gggattcgcg
     2581 gtgtagtcat catgctgact gaaatgaaac agattcatgt caaaggaacg atggtcattg
     2641 cggaagcagg tgcctcactg attgatacga cttatgcagc ttttgcagaa tcattgactg
     2701 gatttgaatt tgcttgcggc atcccgggaa gtgtcggtgg agcggtttat atgaatgcag
     2761 gtgcgtatgg cggagagatc aaagatgttt ttgctgaagt ggatcttttg cttgaagatg
     2821 gaacattgaa aactttgaca aaagacgaaa tggtattttc ttatcgtcac agtaaagtcc
     2881 aagaattacg tgctatcgta ttggaagctc gattcagttt gcaaacaggg gattatgaag
     2941 caatcaaagc aagaatggat gagttgaccg aattgcgtca atcaaaacag ccattagaat
     3001 acccttcatg cggaagtgtc tttaagcgcc cagtaggaca ctatacaggt cagctgatcc
     3061 aacaagcagg attgcaaggc ctgaaatggg gcggtgcaca agtctctgaa aaacatgctg
     3121 gattcatcgt caatatcgat catgcgacag cgacagatta tattgaattg attgcgcaca
     3181 tccaagaagt aatcaaagaa aaatttgatg tgtctttaga aacagaagtg cggatcattg
     3241 gagaaaaaca agcaatatca gaaacaagca gaagtttagc caattaataa gtaatggaac
     3301 ttaaaataag taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc
     3361 aaatgacgca aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc
     3421 ataaattttt ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga
     3481 tgaaaaaaca agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct
     3541 gcatatcaag taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc
     3601 ttatagaaga ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa
     3661 agaattatgt agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa
     3721 gaaattatat catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc
     3781 aaatttcaga agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt
     3841 taagaagaat ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt
     3901 caaaagtaac gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg
     3961 taaaaagaag agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca
     4021 tattagttgg ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct
     4081 ttaatggttt ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc
     4141 aatattttga atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt
     4201 tgctaaccac tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct
     4261 tttcagatga atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata
     4321 tttcttttct tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt
     4381 ttttgatcac ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt
     4441 ttcatgatat ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat
     4501 tttcgattga aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta
     4561 tgatgcatgt caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta
     4621 aaaagcagct acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg
     4681 tcatacaaga gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat
     4741 tttatgatta tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga
     4801 ttcatgtctg tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag
     4861 tgaaaggatt taaaaattac aatattgtga ttg
//
LOCUS       DAZUMM010000123         4888 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000123 DAZUMM010000000
VERSION     DAZUMM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.129"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(145..459)
                     /locus_tag="ACG8E6_002536"
     CDS             complement(145..459)
                     /locus_tag="ACG8E6_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062895.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            745..984
                     /locus_tag="ACG8E6_002537"
     CDS             745..984
                     /locus_tag="ACG8E6_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062896.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1346..1606)
                     /locus_tag="ACG8E6_002538"
     CDS             complement(1346..1606)
                     /locus_tag="ACG8E6_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062897.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1791..2288)
                     /locus_tag="ACG8E6_002539"
     CDS             complement(1791..2288)
                     /locus_tag="ACG8E6_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFS5062898.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(2418..3128)
                     /gene="budA"
                     /locus_tag="ACG8E6_002540"
     CDS             complement(2418..3128)
                     /gene="budA"
                     /locus_tag="ACG8E6_002540"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFS5062899.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(3141..4805)
                     /gene="alsS"
                     /locus_tag="ACG8E6_002541"
     CDS             complement(3141..4805)
                     /gene="alsS"
                     /locus_tag="ACG8E6_002541"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFS5062900.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
BASE COUNT         1475 a         1001 c          749 g         1663 t
ORIGIN      
        1 tataaactct agagaaatta attcatccta acataaaata attgttcact caaaagtttt
       61 tctagaacaa ttaaaacagc aggcttcaca ttcgctatac atttgtaaga agtaataatt
      121 cctgtactaa aaggcataat tgctttaatt attttttgac ttctcttcaa acacaagaag
      181 tagttgtgta ataattgcag aaataaggat tccaataata gatataaaat ttgacatgga
      241 aacgtctata acattaaaag aataaagtgt tgaagaaagt aaactgattg aaataacccc
      301 tccgtaaaaa atagtctgtg aatataccca ctttttttca gaagacgtac ttcgtttggt
      361 tctaaaacct atccctgcat ttctactctt atcaaaaaac caaatattta gaagtaataa
      421 tattgctatt attaagataa aattactgta aaaatccaaa agctatcctc ctagcaacta
      481 tttaaatacc caaaaaaatt acctctagat aatatcatac ctacgttttc acttataaat
      541 tttttcataa aaaaagcgtt tagaggcata atcgttaata cagatggtaa ccaatgtatc
      601 tggtttgaac tgggatatca ccctctccat atatgtatta ctaagactaa tagatactat
      661 aaaagacttg atagttttgc attgattttg cctaatactg agcataggtg tatgctaaac
      721 gatactataa ggagtgtgta cataatgaaa agaattctgt ttatcagttt atcaaattta
      781 ttgttactta ctgcttgtgg gacttctgca tcaaagaaca taccttcaga tacgcaaatt
      841 tcgaatagta tcgattcaac aatttctaca aatgagaaaa attctccggt agaaacttcc
      901 gatataaaag tttctacctc tgcaaaaaac agggtaaaga aactgtcagc acgtctcctt
      961 tatttcaaac aaactctgaa ttagcttctt catcttcttc agcaaataat gatgatagtt
     1021 tcgcgtttac tcctaactaa atgcaacctt tacaaatgct ttacgataac aagcctgagc
     1081 taaatgatcc tgatatcagt tttgcatttt ttgaaatgat tggatcggat gatcttttta
     1141 aaactactag tttgtctatc cgaaaatagg acagttctgg cagtataggt ttttatcgag
     1201 tttctccacc aggcctcgtt acagagacag attctctagg cgaacctttt gaataaatca
     1261 aaaatcatga acaatcactg ccaactaaat aaagtgcctt caaaagcttg gtcttctcgc
     1321 ttttgaaggc actttattta aatctttatg aaactaaagt atgttcatat ttgttgctat
     1381 agttatatag atattctgtt gtttccaact cataaacgtg agtatatttt gccactactt
     1441 ttccgcattt ttctaatatt tcattgcgat cgacgtcatg atattcgtct acgcagatca
     1501 caacacaatt ttctaattta ctgattactt ctccaatcac tttttgtttc aagccaatag
     1561 gttggcaaat atattttttt cctaaaatga tagtttcgtt attcatattt catccatcct
     1621 taatgttttt tcattattta aaatgataat aacattaaaa tatgattatg aaaatcaata
     1681 acacttgtga ttttataaaa aaataaaaaa accgaaaaac agaaatgccc actgtctttc
     1741 cggctctttt tattcttgat ttctcttctt ttttgtttca atagacgatt ttagttatta
     1801 ataaatgaaa aagcatttat attaaaattt gctaataaaa gatctacata tttttctaga
     1861 aatgcttgat cgatttcatc ataccctttc gttactccgc aatcgatatc taaaacaccg
     1921 attaaattac cttcttttac cattggcact acgatttctg attgtgcggc tgaatcgcag
     1981 gaaatgtaat ttgcatgggt tttgacatca tcaacgatca atgtttcttg tttttcagct
     2041 gattctccac aaactccttt tcccatcttg atacgtgtgc aagaaactcg cccttggaac
     2101 ggtcctaaaa tcaattcatt accatcgaat aaataatatc ctgcaaagac agtctcaggc
     2161 aaagtctcat tcaacaatgc agatgaattt gctaaatttg ctaaccagtt atcttctgct
     2221 tctaataatc cttgttgctg taataataaa atttcgtatg catctttttt ctgtttttct
     2281 gtccacatga taaaaaccgc tcctttttga tctatggaga agattatagc tcagtttttt
     2341 tgtaaacgct aggttttctc ctcaaaaaaa cgagatacgt caggttaatc accttgaacg
     2401 catctcgctg tctttttcta ttctgcttgt tggatttctg ctgctaaatc agcataatca
     2461 atcgtttcgt gaacaaaagt cggatcatca actggaaaat gctgtacaag ggacgagatg
     2521 cggctaatct caacttttcc ttcagccact tcaaaatcca ttacatgtcc gccaaatgta
     2581 tgtgtatcat cgataaaatg caggtgaaat cccgctgcag caactccttg gaataattca
     2641 ggcgtaaaga acccgactaa tgttccttgg acattgcttt gttggaattc tggttgagac
     2701 tctgaaattt ttgctagtct tctgtaaggt ttttcttgct tcggcatgat acggatgtgc
     2761 atatttttga ataatccagt tatcttcact gccaaaaata ggttttcccc ggcttctctt
     2821 actaatatgt cttttttcaa ttgttctcca tctgcagttt ttgagacaga aaaactagta
     2881 tctggcgtaa aatctgtaat tgctgcatag ggggtaagtt cacttccatc taattgggca
     2941 actgagccat ctgatcttcc ttgataagct acgccatcta aaaagatgac ttcaccatca
     3001 agcccatgca atgttcccaa tcccagagtt cctttttcta atatatgagc aatcgtttct
     3061 gttccatcca tcaagccggc catcaaagct cctaatgtcc cgtgctggta taagattttt
     3121 tcagtcatgt cgacacttcc ttaatagaat tgatctggta agattgtttc tcccaatttc
     3181 tcattatcac ggtaatcaat cggaatatca ataattacgg gtccttctgt cgcaaatcct
     3241 tcttctaaag ctttttctaa ttcctctaca cttgtagctc ggattccttt agcgccaaaa
     3301 gcttctgcat atttcacaaa atcaactgga ccaaagtcta cacctgacgc ccgctgatat
     3361 ttcatttttt cttggaactc taccatattg tagtgtccat cattccaaat caaatgaacg
     3421 atattcaatt ttttacgtac cgccgtttct aaatcctggg cagagaataa aaatccccca
     3481 tcgccggaaa ctgatacgat ctgtgtattt ggacgcacca atgcagcaga aatcgcccat
     3541 ggcaatgcaa caccaagcgt ttgcatacca ttactgaata acaaatgacg aggttcataa
     3601 cttctaaagt ggcgtgccat ccaaatataa tgactaccta catctacggt aacagtcata
     3661 tcatctgtta cttttgactg aagcgtgtta ataacttcta aaggatgcaa gatacctgct
     3721 tctccttttg attgaacaat gtcccgctcc gttaattttg cttgaagaga tgctaaatat
     3781 tcttttgcat cttctgacac ttgttgaggc tctaatgaac ctgtcaataa atcaagagtg
     3841 gctgagatat caccgatcaa ttctctttca ggctgcataa aaggatcgat ttcagctggc
     3901 gcttcatcga tcacaatgat ccgcgcatct ttttccgcat tccagtttct tgcttcatat
     3961 tcgattggat catagccaat tgcaataact agatcacttc gtttcaaaag catatctcct
     4021 ggttggtttc taaataaacc cacacgacca aagaaatgcg cttctaattc acgtgagatc
     4081 actccagctg cttggaatgt ctctacaaca ggcaatgctg tcttttcaac taattgcctg
     4141 atggctaacg tttctttttc acttgatcct cgcataccta ctaatagtac cggtagtttt
     4201 gcttctctaa tgcgttcagc taggtaacgg atatcatctg cagaagcaga acctaattta
     4261 ggatcgctca atggcttgat gacattacct tggacaggtg catccactac atcttgcggg
     4321 atactgataa agctcgcgcc tttttttgaa ctcttagcca tacgataagc attggcaatc
     4381 acttctgaaa ttgtttctgg atcttggatt tctgcactgt attttgtgat tggctggaac
     4441 agtgctgcat tatccatgct ttgatgggtc aacttcaata gatcgcttcg ttttacttgt
     4501 ccggcaattg ctaaaactgg gtcaccttct gctgtcgctg ttaccaaacc tgtagctaag
     4561 ttactagcac caggaccact tgttgcgata actactcctg gctctcctgt aatacgtccg
     4621 attgcttgtg ccataaatgc agcattttgt tcgtgacgag tcacgattaa ttcaggacct
     4681 tgatcctcta attcattaaa tacaccatcg atttttgcac ctggtatacc gaatacataa
     4741 tcaacatgat gattaatcaa actttctact acagatttcg aaccttgttt cattttttca
     4801 gccattgaac atcgtccttt ttatgcttta tttttaatta ttagttagaa ataatcacaa
     4861 ataagtgata cagtttctaa acctgttt
//
LOCUS       DAZUMM010000124         4829 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000124 DAZUMM010000000
VERSION     DAZUMM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.130"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..709
                     /locus_tag="ACG8E6_002542"
     CDS             116..709
                     /locus_tag="ACG8E6_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFS5062901.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKEAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDEYAIQPHYTNKENFDLGITETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            725..1624
                     /locus_tag="ACG8E6_002543"
     CDS             725..1624
                     /locus_tag="ACG8E6_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFS5062902.1"
                     /translation="MTPTQFKAKTRNFATEKHLNPQIVQRYYMMEKLLERIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTADPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERINEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKVERKQNFKRSQQMNQEMER"
     gene            1627..2112
                     /gene="ssb"
                     /locus_tag="ACG8E6_002544"
     CDS             1627..2112
                     /gene="ssb"
                     /locus_tag="ACG8E6_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS5062903.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTATFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            2157..2390
                     /locus_tag="ACG8E6_002545"
     CDS             2157..2390
                     /locus_tag="ACG8E6_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062904.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGRDNSFWRLTKVTIAATAVILTAILPLYM"
     gene            complement(2549..2671)
                     /locus_tag="ACG8E6_002546"
     CDS             complement(2549..2671)
                     /locus_tag="ACG8E6_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFS5062905.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            2826..3086
                     /locus_tag="ACG8E6_002547"
     CDS             2826..3086
                     /locus_tag="ACG8E6_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062906.1"
                     /translation="MDKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            3529..3735
                     /locus_tag="ACG8E6_002548"
     CDS             3529..3735
                     /locus_tag="ACG8E6_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFS5062907.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     QTCGKKYTLSYGDYVLIDEKGKKH"
     gene            3735..3986
                     /locus_tag="ACG8E6_002549"
     CDS             3735..3986
                     /locus_tag="ACG8E6_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062908.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            4002..4439
                     /locus_tag="ACG8E6_002550"
     CDS             4002..4439
                     /locus_tag="ACG8E6_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062909.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
BASE COUNT         1733 a          810 c          956 g         1330 t
ORIGIN      
        1 aaccagcagc ggattcaaaa agaaattgaa ttagatcgat aaatttcttt cttgttaata
       61 taaaactaac atgttatagt tatgttagtt ttatattaac tttaggggaa aagcaatgaa
      121 agacaaaatc cagtgtattt ttgataaaca taatgggaat ctatccttga aggatgctag
      181 aagtgaagga ttatcgccta tgacgttaga tcgattagcc gaaaaggaca aaatagaaaa
      241 ggtagcacca ggtttctata ccttaaaaga agcgttcgtg gatgaattgt atctcgctca
      301 aagtatctat tccagaggga ttttttctca tgagactgcg ctcgatcttt ttcaagtagg
      361 aacgtatatc cctaaaaaga tcaatatgat gtttcctaaa ggatacaatg tttcaaagga
      421 gaaattagac gagtatgcta ttcaaccgca ttacacgaat aaagaaaatt ttgacctggg
      481 aatcacagaa acagaaagct tttacggcaa taaaatttta gtctatgatt tagagcgaac
      541 gttatgtgac atgtggaatc caagatacaa agcaagcgta gaggtaaaac aagaagcatt
      601 aaaagaatat atgacaagta ccagtagaaa tacaaataag ctaagaagat acatggatat
      661 tttaaaatca ccaaaagaaa tgactttgta tatgttgcca ctttattgag aggagtaaag
      721 gtgaatgaca ccgacgcaat ttaaagcaaa aacgagaaat tttgcaacag agaaacattt
      781 aaacccacaa attgtccaaa gatattatat gatggaaaaa ttattagaac gtattgttga
      841 atcgccttat catgatgatt ttgtattaaa agggggattt ctgatcggat caaaatacgg
      901 gttagaaaac cgttcaactg ttgatattga tacgacttat cgaaattcga aactaacaaa
      961 agaaaagtta gaaactgttt tttctgaatt aacagctgat cccacaaaag atggaattcg
     1021 atttgaatta aagggattaa cagagacgag ggaagctgat tattacccag gttttcaagc
     1081 aaagtttatt gcttatcttg agaatgcacg tgtaccattt aaattagata ttacttcagg
     1141 tgattctatt attccagcgg cattgatgta tgaacacaaa ttaatgtttg aagataaaac
     1201 gattcatatt ccagcttatc ctacggaaca aattctagct gaaaaattac atgccacgtt
     1261 tagttttgga aaagataatt cgcgcatgaa agatttttat gacctgtata tgattccgaa
     1321 attagaacgt atcaatgaga aaaatttgta taaaagtgtt caaggaacat tcaaggaaag
     1381 aaataatcaa tcggtgttta agcatttgta tgaccaggaa atgcctcaat tggtgaagga
     1441 tgaggggttg agggaaaagt ggaaaagata ccaacaagaa aactattttg ccaaagacat
     1501 ttctttcgaa tctacactaa cttccgttga ttccctgatg caatcaattg tagagcaaga
     1561 gaaggtagaa cgaaaacaaa attttaagag atcacaacaa atgaaccaag aaatggagag
     1621 ataaagatga ttaataatgt aactttagta ggacggttaa ccaaagatcc agatttacgt
     1681 tataccgcaa gtggcacagc agtagcgact tttaccctag ccgtcaaccg aaatttcgcg
     1741 aatcaaaacg gcaatcgaga agccgatttt attaactgcg tgatttggcg caaaccagca
     1801 gaaacaatgg cgacattagc taaaaaaggc ggcttgatcg gtgttgtcgg acggattcaa
     1861 acacgaactt atgacaacca gcaaggacaa cgtgtgtatg tgactgaagt tgtagcagat
     1921 aactttcaac tattagaaag taaagcagcg acagagagcc gtgcgcacgc tgatcaatcg
     1981 tccaccagtc caagcaccgc cacctttgaa caacgggata cagcgacgcc taacaacaat
     2041 ggattgaacg cttcacagaa tccatttggt ggacaatcaa tcgatattag cgatgatgat
     2101 ctaccgtttt agttcgagaa agaaggagta agcatggcaa aaaaaatagt ctgacgatgg
     2161 atctattgat acccgtaatt gcatttatag acgggatttc tttgggcgtc actgaaaatc
     2221 ctttggtaaa tagaattgcg aaaggatcac tatttcttgt attcgtgtat cttgtatgtt
     2281 cttatctttt aaaaagagca ggaagggata attccttttg gcgattgact aaagtcacaa
     2341 tagcagcgac agctgttata cttacggcta ttctaccgct ttatatgtag ggttaacggg
     2401 aaatcgttga acttcttgcg aaatattgct ataatgaaca tgaaaaagag gagttgcgct
     2461 aacaacccct cattaacagc accgattaaa gaacggtggc agaatattag ttatttggaa
     2521 ataaccgctg acctgctaaa gtggacggtt atttcttttt gtcattttta agcatattta
     2581 caatcaaact aatcaaagca atggtaaaca taccaaaacc aagaatggtt tgaattgtct
     2641 catatgcgga caaaaggctt actcctttca tagattttag cacttacatt cataagcacc
     2701 accacctttc cagaagtcgc caccgtcttc aaactttact gtcttgacta ttatagcata
     2761 tctaaaatcg aataaaaatt ctcaattttg gttgtttttg ctataatttt agcgaggtga
     2821 ttgtgatgga caaatatgtt ggtttgcttg ataaaatccg agtaataaaa acacaaccat
     2881 tattggttcg ctttacttta caaacaattc atgaatcgat taattgtgta gttgcagata
     2941 ttgaaattat cgataaactg ctaattatgg atgatggaaa atacaatatt gccgtcacag
     3001 gtcacttcaa taaacgaaat cagctagtta ttgctagcat gtatgttaga aatcccgatc
     3061 attttacgcg atcgatgggc atataaaaga agagttgtag tcctaaatga ggattgcagc
     3121 tctttttgtt ttacatagaa acgggaacag gaaaaggcct gtttaggaag gcacaagcgc
     3181 cttctcaatt gaaaaacctt tctctcactc caaccgccac cgccgttcga gaagaaagga
     3241 agtgaacgtt gcacgttcac taaaataagg ggtcttgcaa agcaagacaa aataggaaaa
     3301 acttcctctc accaaaaggt tcgttcctgt tcgctatctc tttattgatt tgccagaccg
     3361 gcaacaagtt gccttttatc accaaaaagc gacaaggaca agggtaaatg gcacgctgcg
     3421 cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc aacaaatcaa taaagaaaga
     3481 actaacaaaa ggaagtaaac cctttcctaa gaggaggcag aatcaatcat gcgaaagaaa
     3541 tttgattatt ggggtgttcc cttcagcgaa ttattcccaa actatcatgc accacatact
     3601 gtcgaatgcg attgtggaga aagagcgaaa tgtatcaaaa gctatcgttt gtatcaatgt
     3661 caaacatgcg ggaaaaaata taccttgtct tatggcgatt atgttttgat tgatgagaaa
     3721 ggaaagaaac actgatgaaa acacaacaca aattattgac tgtaaaagaa gtcgttcaaa
     3781 ttttggtgga caaagaagtc atttctgaaa aagtagcaga agctttactt attccgcggt
     3841 actggacgat agaaacccct ttagtagagg atgcaccgac tatgctattt gaagacctag
     3901 tgaagattgc aggatatgaa ctgcattatc aagaaaacat ggaaacttat gaaaacgaat
     3961 attggttaac caaattagag aactaaaaag gagaaaaaaa tatggctcaa aattactatg
     4021 acgaatttgt caaattacct ttggataaaa tggcacaaaa aatggaggat atgacgttcc
     4081 tatacaacga aacacgtgtt ccaaagaaac attataaaga aaaattgtcg gtagctgtgg
     4141 aagaaatgat tgaatcaggt gtcgaaatga atctcattgc gacgtactat cgaacactgg
     4201 aagaactgaa aaagcaaaat gcaaaatggt ttttccaagc cttactatgc ttggaagtag
     4261 gcgtcaaacc aagcacaatc aaaccttctg aataccaagc tttagaacta acgtatgcga
     4321 aatttatcga aacgaaaaaa gccaagactg tttcaagtga atggttagat tattttgaaa
     4381 atatcaataa gtatggcgca tattacacca tgaaaaagga agacaacgaa aatgaataag
     4441 gtgatttatc aactaacaac ggattccata tcaacgattt gaaagaaaaa aaggtcaata
     4501 atcatttata ccgaaaagtg agaatgtgtg aaataaggac aaaaaaagcc tagtaactaa
     4561 gggaatggtt cttatttcgc accaatcgct ccaaccgccc ccgccgctta ggtgatgcga
     4621 aataaacggg caaagcccga taactcaaga gaaagaaggt gatccatttg aacaagatta
     4681 ttcccttatt gttagtacta tttgttggcg aaataattat gcgacatagt gcagctgtgc
     4741 atttaaactt cgtagtattt ttggtaatac tggtgttttg tttgatcttt tggcgctttg
     4801 tgttagccct tgtgagtcta atattcaag
//
LOCUS       DAZUMM010000125         4804 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000125 DAZUMM010000000
VERSION     DAZUMM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.131"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..411
                     /locus_tag="ACG8E6_002551"
     CDS             <1..411
                     /locus_tag="ACG8E6_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062910.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     ELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            509..1882
                     /gene="tet(L)"
                     /locus_tag="ACG8E6_002552"
     CDS             509..1882
                     /gene="tet(L)"
                     /locus_tag="ACG8E6_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HFS5062911.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            2446..3708
                     /gene="mobV"
                     /locus_tag="ACG8E6_002553"
     CDS             2446..3708
                     /gene="mobV"
                     /locus_tag="ACG8E6_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HFS5062912.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            3953..>4804
                     /locus_tag="ACG8E6_002554"
     CDS             3953..>4804
                     /locus_tag="ACG8E6_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFS5062913.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1657 a          665 c          981 g         1501 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
      361 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
      421 tgttgttata taaatatggt ttcttgttaa ataagatgaa atatttttta ataaagattt
      481 gaattaaagt gtaaaggagg aattaattat gaagtgtaat gaatgtaaca gggttaaata
      541 caaccaaatt ttaatttggc tttgcgtttt atcttttttt agcgtattaa atgaaatggt
      601 tttgaacgtc tcattacctg atattgcaaa tgattttaat aaaccacctg cgagtacaaa
      661 ctgggtgaac acagccttta tgttaacctt ttccattgga acagctgtat atggaaagct
      721 atctgatcaa ttaggcatca aaaggttact cctatttgga attataataa attgtttcgg
      781 gtcggtaatt gggtttgttg gccattcttt cttttcctta cttattatgg ctcgttttat
      841 tcaaggggct ggtgcagctg catttccagc actcgtaatg gttgtagttg cgcgctatat
      901 tccaaaggaa aataggggta aagcatttgg tcttattgga tcgatagtag ccatgggaga
      961 aggagtcggt ccagcgattg gtggaatgat agcccattat attcattggt cctatcttct
     1021 actcattcct atgataacaa ttatcactgt tccgtttctt atgaaattat taaagaaaga
     1081 agtaaggata aaaggtcatt ttgatatcaa aggaattata ctaatgtctg taggcattgt
     1141 attttttatg ttgtttacaa catcatatag catttctttt cttatcgtta gcgtgctgtc
     1201 attcctgata tttgtaaaac atatcaggaa agtaacagat ccttttgttg atcccggatt
     1261 agggaaaaat atacctttta tgattggagt tctttgtggg ggaattatat ttggaacagt
     1321 agcagggttt gtctctatgg ttccttatat gatgaaagat gttcaccagc taagtactgc
     1381 cgaaatcgga agtgtaatta ttttccctgg aacaatgagt gtcattattt tcggctacat
     1441 tggtgggata cttgttgata gaagaggtcc tttatacgtg ttaaacatcg gagttacatt
     1501 tctttctgtt agctttttaa ctgcttcctt tcttttagaa acaacatcat ggttcatgac
     1561 aattataatc gtatttgttt taggtgggct ttcgttcacc aaaacagtta tatcaacaat
     1621 tgtttcaagt agcttgaaac agcaggaagc tggtgctgga atgagtttgc ttaactttac
     1681 cagcttttta tcagagggaa caggtattgc aattgtaggt ggtttattat ccataccctt
     1741 acttgatcaa aggttgttac ctatggaagt tgatcagtca acttatctgt atagtaattt
     1801 gttattactt ttttcaggaa tcattgtcat tagttggctg gttaccttga atgtatataa
     1861 acattctcaa agggatttct aaatcgttaa gggatcaact ttgggagaga gttcaaaatt
     1921 gatccttttt ttataacagg aattcaaatc tttttgttcc attaaagggc gcgattgctg
     1981 aataaaagat acgagagacc tctcttgtat cttttttatt ttgagtggtt ttgtccgtta
     2041 cactagaaaa ccgaaagaca ataaaaattt tattcttgct gagtctggct ttcggtaagc
     2101 tagacaaaac ggacaaaata aaaattggca agggtttaaa ggtggagatt ttttgagtga
     2161 tcttctcaaa aaatactacc tgtcccttgc tgatttttaa acgagcacga gagcaaaacc
     2221 cccctttgct gaggtggcag agggcaggtt tttttgtttc ttttttctcg taaaaaaaag
     2281 aaaggtctta aaggttttat ggttttggtc ggcactgccg acagcctcgc agagcacaca
     2341 ctttatgaat ataaagtata gtgtgttata ctttacttgg aagtggttgc cggaaagagc
     2401 gaaaatgcct cacatttgtg ccacctaaaa aggagcgatt tacatatgag ttatgcagtt
     2461 tgtagaatgc aaaaagtgaa atcagctgga ctaaaaggca tgcaatttca taatcaaaga
     2521 gagcgaaaaa gtagaacgaa tgatgatatt gaccatgagc gaacacgtga aaattatgat
     2581 ttgaaaaatg ataaaaatat tgattacaac gaacgtgtca aagaaattat tgaatcacaa
     2641 aaaacaggta caagaaaaac gaggaaagat gctgttcttg taaatgagtt gctagtaaca
     2701 tctgaccgag atttttttga gcaactggat ccaggagaac aaaaacgatt ttttgaggaa
     2761 agttataaat tattttccga acgatatggc aagcaaaata ttgcttatgc aacagttcat
     2821 aatgatgagc aaacccctca catgcattta ggtgttgtgc ctatgcgtga tggaaaactg
     2881 caaggaaaaa atgtgtttaa tcgtcaagaa ctgttatggc tacaagataa attccccgag
     2941 catatgaaaa aacagggttt tgagttgaag cgtggtgaac gtggctctga ccgtaaacat
     3001 attgagacag ctaaatttaa aaaacaaact ttggaaaaag agattgattt tctagaaaaa
     3061 aatttagcag ttaaaaaaga tgaatggact gcttatagcg ataaagttaa atcagattta
     3121 gaagtaccag cgaaacgaca catgaaaagt gttgaagtgc caacgggtga aaagtccatg
     3181 tttggtttgg gaaaagaaat aatgaaaaca gaaaagaaac caaccaaaaa tgttgttata
     3241 tcggagcgtg attataaaaa cttagtgact gctgcgagag ataacgatag gttaaaacag
     3301 catgttagaa atctcatgag tactgatatg gcgagagaat ataaaaaatt aagtaaagaa
     3361 catgggcaag ttaaagaaaa atatagtggt cttgtagagc gatttaatga aaatgtaaat
     3421 gattataatg agttgcttga agaaaacaag tctttaaagt ctaaaataag cgatttaaag
     3481 cgtgatgtga gtttaatcta tgaaagcact aaggaattcc ttaaggaacg tacagacggc
     3541 ttaaaagcct ttaaaaacgt ttttaagggg tttgtagaca aggtaaagga taaaacagca
     3601 caattccaag aaaaacacga tttagaacct aaaaagaacg aatttgaact aactcataac
     3661 cgagaggtaa aaaaagaacg aagtcgagat cagggaatga gtttataaaa taaaaaaagc
     3721 acctgaaaag gtgtcttttt ttgatggttt tgaacttgtt ctttcttatc ttgatatata
     3781 tagaaataac gtcattttta ttttagttgc tgaaaggtgc gttgaagtgt tggtatgtat
     3841 gtgttttaaa gtattgaaaa cccttaaaat tggttgcaca gaaaaacccc atctgttaaa
     3901 gttataagtg accaaacaaa taactaaata gatgggggtt tcttttaata ttatgtgtcc
     3961 taatagtagc atttattcag atgaaaaatc aagggtttta gtggacaaga caaaaagtgg
     4021 aaaagtgaga ccatggagag aaaagaaaat cgctaatgtt gattactttg aacttctgca
     4081 tattcttgaa tttaaaaagg ctgaaagagt aaaagattgt gctgaaatat tagagtataa
     4141 acaaaatcgt gaaacaggcg aaagaaagtt gtatcgagtg tggttttgta aatccaggct
     4201 ttgtccaatg tgcaactgga ggagagcaat gaaacatggc attcagtcac aaaaggttgt
     4261 tgctgaagtt attaaacaaa agccaacagt tcgttggttg tttctcacat taacagttaa
     4321 aaatgtttat gatggcgaag aattaaataa gagtttgtca gatatggctc aaagatttcg
     4381 ccgaatgatg caatataaaa aaattaataa aaatcttgtt ggttttatgc gtgcaacgga
     4441 agtgacaata aataataaag ataattctta taatcagcac atgcatgtat tggtatgtgt
     4501 ggaaccaact tattttaaga atacagaaaa ctacgtgaat caaaaacaat ggattcaatt
     4561 ttggaaaaag gcaatgaaat tagactatga tccaaatgta aaagttcaaa tgattcgacc
     4621 gaaaaataaa tataaatcgg atatacaatc ggcaattgac gaaactgcaa aatatcctgt
     4681 aaaggatacg gattttatga ccgatgatga agaaaagaat ttgaaacgtt tgtctgattt
     4741 ggaggaaggt ttacaccgta aaaggttaat ctcctatggt ggtttgttaa aagaaataca
     4801 taaa
//
LOCUS       DAZUMM010000126         4757 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000126 DAZUMM010000000
VERSION     DAZUMM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.132"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACG8E6_002555"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACG8E6_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFS5062914.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="ACG8E6_002556"
     CDS             complement(2064..3062)
                     /locus_tag="ACG8E6_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFS5062915.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="ACG8E6_002557"
     CDS             complement(3288..3941)
                     /locus_tag="ACG8E6_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFS5062916.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACG8E6_002558"
     CDS             complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACG8E6_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFS5062917.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1395 a         1001 c          743 g         1618 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcta attgtcgtac
     4321 atcgcgcggt aggaaccgac gaatttcatc ttcattaaaa ccaacttgca gacgtttatc
     4381 atcaatcatg attggacggc gaagcaatcc aggattgttt tgcacaagtt ccaataattc
     4441 ttgtaacggc agatcatcta aatccatgtt cagtttttgg aatactttgg aacgtgtcga
     4501 gatgatttct tctgttccgt cttctgtcat ttgtaaaatc gcttttaatt cagaactgtt
     4561 caatggttct gaaaaaatat tacgttcaac aaatggaatc tgatgttctt gcaaccacgc
     4621 acgagccttt ctgcaggaag tacaacttgg ggaagtatat aatgttaaca acgtgcacca
     4681 ctccttttcg tgataagaat cacttttttg taacagaaga tcataataat ctcccatctg
     4741 tttctattat acataat
//
LOCUS       DAZUMM010000127         4675 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000127 DAZUMM010000000
VERSION     DAZUMM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.133"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..2376
                     /locus_tag="ACG8E6_002559"
     CDS             4..2376
                     /locus_tag="ACG8E6_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFS5062918.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIREEIERFEKGESSFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGEFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQSELQAGIQAVANNIGIQIRDITESEIYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKLSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVQQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="ACG8E6_002560"
     CDS             2437..3897
                     /locus_tag="ACG8E6_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFS5062919.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEAKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLITELKPKHERQNF
                     LVRDNRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVHCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFDQQLSVNDSIEAINLKD
                     ICREVAKELKGCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAILFQQGRQQERE"
     gene            3908..>4675
                     /locus_tag="ACG8E6_002561"
     CDS             3908..>4675
                     /locus_tag="ACG8E6_002561"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFS5062920.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKTTRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYRYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTYKRLWINSLEKEAIYQGFQQLRDAGETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGISGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKAQVTAQNGTFDAKLDPNETQVTQEAFEA"
BASE COUNT         1785 a          777 c         1010 g         1103 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaatgaa caaatcgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gttataacaa gtacggcgga
      301 ctagatatga cgatcgaaaa tattccaaga atcagagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgt ctttttcagc cgccacgatc cgaaaatatc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt aacgcaatgg aaaaaaaatc cgaccattta ttttctgaaa
      541 ggctatcgaa aagtcgcttt agaactgaat gaaaacgggg aatttgagga atctaaaaaa
      601 taccaagcca catcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgcg
      661 ccaactgatt ctctgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggagaga tcgtaacatt agaattgatc
      781 gatcaactca aaacgttgga caaccaatac gctgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaaacggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggatatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatcac tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaactcag ttaaaacctg aagactatcc gaaaatcttc
     1501 ccgaaccgtg tctttgattt tcaattggat caaacagggc agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tatcgggatt caaattcgag atattacgga gagtgaaatc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaactt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 cagcaggagc ataaaaatac tgtagaaacg gcagaacaaa agaatattgt gcctgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcca aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgc gtataaaaca agtaagaaag actatctgaa
     2401 aaatggatag tcttttttct tttgggaggt ttgaaaatga cagagaaaaa aatgagtctc
     2461 attgatcgat gcaagcaaat ggatattgtg gattttgcac gaaacaatgg aatggctgta
     2521 gtcaataaag gacgtgacta tcgcttagaa gaccatgatt cgtttgtttt cgatcgaaga
     2581 aaacaacgtt tttattggaa tagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttta tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaagc caaaacagaa cgagtagaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccacttacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaattgcc agagtggctg attgattatg cagaaaaaga aggattgatt
     2881 actgaactaa agccaaaaca tgaacggcaa aattttttag ttcgagacaa tcgtttagat
     2941 catgcagttg cctttttatg gaaagatcca cagacaaaag agactgtggg ggcgagttat
     3001 caaggaacgt ttattgatta tgaacgtttt ggtgagagag gaacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagttaaaa
     3121 ttttttgaga gtagtattga tttacttagc tacgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatcgattga gatttgtgtg
     3301 gataatgatc gtgcaggaca tattttctac gaaaaggaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acattgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtca atgcgtcttt tgaccagcag ctttctgtta acgattcaat agaagctatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aaggttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc tcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtga aaaaggattg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat attgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaaaccgtca caagcgaagg cttatgccga
     3961 tagcttttct aaaaccaccc gaaaagacgg gtattttgag atccaagatc gattgttttc
     4021 gggagaaact gtgatcacgt atggttttgg tcatttagtt gagttagatt cgccagatat
     4081 gtacgatgaa aattggaaac aatggtcatt agagcatttg cctattttcc ctacacatta
     4141 tcgctatcat gttcctaaag ataagaaaaa acaatttaac gtagtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatagcttg
     4261 gacgatcatt caacaagcag gtgcagacca gggaaaaacc tataaaagac tttggatcaa
     4321 ctccttagaa aaagaagcga tttatcaggg attccagcag ttacgagacg ctggagagac
     4381 ctatccaaaa tttgaggaag ctcaagcacg tcagatcgct gattggttga tcggcatgaa
     4441 cggtagtcca ctatatagtt tactcttgca gcaaaaaggc atatcaggaa gcttctcctt
     4501 aggcagagtt cagactccaa cgctttatat gatttatcag ttgcaagaaa agattaggaa
     4561 cttcaagaaa gaaccctact ttgaaggaaa agcccaagta accgctcaaa atggcacatt
     4621 tgatgccaag cttgatccca atgaaaccca agtgacgcaa gaagcatttg aagcg
//
LOCUS       DAZUMM010000128         4574 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000128 DAZUMM010000000
VERSION     DAZUMM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.134"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..310
                     /locus_tag="ACG8E6_002562"
                     /pseudo
     CDS             212..310
                     /locus_tag="ACG8E6_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(533..763)
                     /locus_tag="ACG8E6_002563"
     CDS             complement(533..763)
                     /locus_tag="ACG8E6_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFS5062921.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(993..1811)
                     /locus_tag="ACG8E6_002564"
     CDS             complement(993..1811)
                     /locus_tag="ACG8E6_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5062922.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1972..2661
                     /locus_tag="ACG8E6_002565"
     CDS             1972..2661
                     /locus_tag="ACG8E6_002565"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS5062923.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2675..4177
                     /locus_tag="ACG8E6_002566"
     CDS             2675..4177
                     /locus_tag="ACG8E6_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5062924.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            4190..>4574
                     /locus_tag="ACG8E6_002567"
     CDS             4190..>4574
                     /locus_tag="ACG8E6_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS5062925.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVI"
BASE COUNT         1523 a          674 c          820 g         1557 t
ORIGIN      
        1 ttaaggggca gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg
       61 gtgtatgggt tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg
      121 gtgatgaaat tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca
      181 aaacacaaat tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact
      241 ggattatcga acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa
      301 cttaatttga caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca
      361 ataggcagat gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat
      421 caaaaggatc ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca
      481 gtttgtctca taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt
      541 caacgttgtt cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta
      601 gaacaccctt tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc
      661 gtttagattc agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct
      721 taataaattt atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga
      781 taatgagcta cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt
      841 agataaaatc aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat
      901 tagtaaaaaa taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta
      961 aaaaccgtta ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag
     1021 cgcaaaactt tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga
     1081 actaatagat cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga
     1141 aattcttcaa tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata
     1201 tagttagtta tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa
     1261 gtatctttcg ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa
     1321 gaagcgactt gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga
     1381 agaaacatat tttctaaatc aagactagta tttccactag atcccactcc aaatatatat
     1441 atgttttttg attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc
     1501 ctaatagtgg aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca
     1561 ctagtcggat gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca
     1621 gaaaaaccag aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt
     1681 gtgtttttct taatatcact catcgtacta taaataatag ttttcccaga atttaaaata
     1741 aaatcagcga cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag
     1801 atataactca tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat
     1861 aaaaaaaact cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt
     1921 gtttttggag ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa
     1981 aagaggtttt tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt
     2041 gaaccatttt acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg
     2101 gctggagcag ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt
     2161 gtatcattac caatgattgg aattattaaa agagattatc caccagaatc tccattcatt
     2221 actgccacta tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt
     2281 gattgcactc ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata
     2341 aaagaaaaat atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa
     2401 aatgcatata aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa
     2461 agtaaaaaac aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt
     2521 tgtgtaattg ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata
     2581 ggagttgcag gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga
     2641 tttgttaaag cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg
     2701 acgcgctttc atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg
     2761 aggagcgtta acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt
     2821 gcatacaatt ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact
     2881 aatttttgca attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt
     2941 agctggtgga gtagcttatt tagtatatac tgcaactatt agtgggtttc ttgaactttt
     3001 ttcagcaaaa gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac
     3061 tgttgcattt ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt
     3121 tggaggatca agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt
     3181 cttttattta atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc
     3241 ccaaatgggt agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt
     3301 tggactacac cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac
     3361 tgtagctgga tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc
     3421 aaaccacact ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat
     3481 gatgtttggg ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg
     3541 taaaaagaat ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac
     3601 tgaaccagtt gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt
     3661 cttagatgga ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt
     3721 ttcaggtggt ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa
     3781 ttggttaaaa ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac
     3841 attctttatc aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca
     3901 ttctgaaaaa gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga
     3961 agctcttggt ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt
     4021 tgctgttacg aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc
     4081 tgtcttcgaa gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag
     4141 tcaagaaatc aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat
     4201 tttcaaaaag aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat
     4261 cacagaagta aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat
     4321 acctgatggt tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac
     4381 taaacatgct attaatataa aaagcaactc aggcattgat tatttgattc atattggaat
     4441 tgatacagtt gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt
     4501 tactcctgaa acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga
     4561 tacttctgtt atag
//
LOCUS       DAZUMM010000129         4469 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000129 DAZUMM010000000
VERSION     DAZUMM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.135"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..482)
                     /locus_tag="ACG8E6_002568"
     CDS             complement(12..482)
                     /locus_tag="ACG8E6_002568"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFS5062926.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="ACG8E6_002569"
     CDS             complement(641..1099)
                     /locus_tag="ACG8E6_002569"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFS5062927.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="ACG8E6_002570"
     CDS             complement(1270..2415)
                     /locus_tag="ACG8E6_002570"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFS5062928.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="ACG8E6_002571"
     CDS             complement(2428..3345)
                     /locus_tag="ACG8E6_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFS5062929.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="ACG8E6_002572"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="ACG8E6_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFS5062930.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1378 a          828 c          850 g         1413 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttat
//
LOCUS       DAZUMM010000130         4455 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000130 DAZUMM010000000
VERSION     DAZUMM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.136"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..411
                     /gene="bacA"
                     /locus_tag="ACG8E6_002573"
     CDS             <1..411
                     /gene="bacA"
                     /locus_tag="ACG8E6_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFS5062931.1"
                     /translation="ITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRYNHNHRGFT
                     CYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVDAMNVFV
                     RAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            1701..2624
                     /locus_tag="ACG8E6_002574"
     CDS             1701..2624
                     /locus_tag="ACG8E6_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5062932.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            2640..3179
                     /locus_tag="ACG8E6_002575"
     CDS             2640..3179
                     /locus_tag="ACG8E6_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS5062933.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            3864..4037
                     /locus_tag="ACG8E6_002576"
     CDS             3864..4037
                     /locus_tag="ACG8E6_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062934.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNGELKLNSNYYN
                     SPNEELFVKETDK"
BASE COUNT         1661 a          673 c          758 g         1363 t
ORIGIN      
        1 attacttgtc tatttttgtt cggtatgatt gttggagcaa gtactccgtt aaatctgttt
       61 ccttttagtg ctagaggtat aaaagtggaa gctgcaagtt cacgatataa tcataatcat
      121 cgtggattta cttgctataa aatgaactat tatgttacta ataaaatgtg tagagatttg
      181 aaaaaaaatt ataataagct aaagactcct gctcaaattg catcttttat tcctattggt
      241 ggagttggaa catgggttat tacaaatgct tttagtagtg ctgttgatgc aatgaatgtc
      301 tttgtaagag ctgcaaatca aggaaaaggt gtacaattaa catataatgc tcactttagc
      361 aatacaacat catatcaata caatgattac gctcgttatg taataaaata atcagaaagt
      421 gaaactatct catgaataaa aagattttag ctgtaattat ttcaataata gttttaatca
      481 ctatgttctt catatttagg ctaattttca atataagctt acaaaaaagt attctttatc
      541 taattcctat agcatttatc ttagcaattt ttagaagtat ttacggaaat aaaaactcta
      601 aatagctaaa aataaatcat actaaatttt acaaaaagtg gctagcattt tctagccact
      661 ttttgtattg tggagcgtgg gttttgaggg ggattcaagg gggagcttgc gcccctttga
      721 caagcggtta tttgcaccaa atacggaggt atttggctag caaatataga gcttgccaaa
      781 cccttttagt gagttttgta gaaagtacct ttaaaggtac tttcacatta cgaacgatac
      841 gtccgtagga cgtactgaaa gggtgggact tatgagcaaa ttaatgcaaa atttggctag
      901 caaaaaagct aaaaaatata tgctaataat cgagcgaaat aaacactttt tcaagtgagc
      961 gaatccgcca tgacaaagct gaagcagttt atttcagctt tgtcatggcg tgtgccgact
     1021 tttttaaaag tctaggcaca cgagcaaata tctttatcaa gctctaattt gcgttttaag
     1081 acgtttgaaa cgcctaagta tataaatata taagaatagg tttaagagtg cttaaatcga
     1141 attttagagg gcatttagac aattttaggg ttgttagggc ttgccctgac cgcctgtaag
     1201 gcgttttaag aacaagataa gggatttata ctaggactgt cttaaaatgt cttaaacgaa
     1261 cgctcagagc ttctcaaagg gttccttatg aacggctttt agcaaaggag ccggaggcga
     1321 ctgcgcagta taatgtgaga gcagagcgat cacacctttt tagcgaaccg tagggagcaa
     1381 ggtttggaat tggcagtttt gataaattcg cgaacctgtt tagagaaaaa aggagtgatt
     1441 ttttaggcgg gaaaattagc ctcccctctt tttatatctc tttttatatc tctttttata
     1501 tctcttttat gtcggctgtg tccttactct cccaagcaat tcagagtttt ttcaagtagg
     1561 ttttctacgc ttttcaagta ggttttctac gcttttcaag taggttttct acgcttttca
     1621 agtaggtttt ccgacattat tgagtatttt tataaagtat gttactttta tttcatactt
     1681 tataaaagta ggagaagctt atggcaaatg aaattgtaaa atatgaaaat agattaaatc
     1741 atattcctct gagaaaattt aatagtcgtg aaatgaactt atttttttca attgcttcac
     1801 gtgtacgtga aaaaggtgta gatgagatta ttttttcatt tcacgatttg aaaaaactaa
     1861 gcggttatac agaacgtggg gaacaattta taaaagatct tagtagcacg tatgacaaat
     1921 tattaagtat taatgcttgg aatgatgatg gaaatacttt aactaaattt gttgctttca
     1981 caaaatatag cattatacga gataaacaac ttgttataat ttcagttaat ccagaattta
     2041 aaggactttt taatcaatta aagaactgga caagatttaa tctagagcaa tttactaatt
     2101 tggatagcac atattcaaaa actatgtttc gattattaaa acaatatcga actcaaggct
     2161 ggactgaatt ttccaaagaa aaattttttg atttactaga tattcctaaa agttattgga
     2221 atagtccgtc aaatattgac aaatttatct taaggccaat taaagaagaa ctcactccct
     2281 tatttaaagg attaactatt cgtaaaaaat atggtaaagg tcgaggtaaa cctgttatcg
     2341 gttatcgttt tacttggaaa gctgaaatta accatgctga cgatttttcc aaagggaaac
     2401 aagaagattt acgtataaaa ttatttaata ttgatcacaa tggagaatta acacgagaag
     2461 aaaaatggcg cgctaaagat cgaatactaa atcttccctt aggcactcat gaagctgatt
     2521 ttaaacaaca acaacaaact gaagaaaaac aggcagaaaa acgagaactt tcaaacaaac
     2581 tcaaacaaga tttactagga aatttacaaa acttattcga ttaagcttaa ggagtattta
     2641 tggcagataa aacaatcaaa gaacttgcgg aagaattagg agttagtaaa acagcaatta
     2701 acaaaaaagt ttctgacaaa aatcgaaaac tttggtttgc aaaaaacgga aacaagtttg
     2761 tgattaacga aaccggtcaa aaagctataa aatcaatgtt tttagtcgat aacgcaaact
     2821 taaatcgaaa actagttggc gaaaaatcaa aaaccaaaaa ccataaaaac accaaccaag
     2881 tttccgaatt aaaactcctt tctttcctag aaaaacagtt aaatcgaaaa gatatacagc
     2941 tacaagaaaa agataatcaa ataaaacaaa tgcaaaaatt actcgaccag caacaacaac
     3001 tcactctaca agcgaaccaa caaattgaga acctacaaga acagctccat ttaacttata
     3061 cagaagaaac tccaacaacc gaatcaactg ccttttctga aaaaaaagca gacaatacac
     3121 aaccacttat tgaaaaaaag tggtggcaaa tttggaaatc taatagaacc aatctctaaa
     3181 attttctaga agttagttcg gatagtagag gaaaaagttg tactacctga tggggagatc
     3241 actcaaattt ttatgagaaa gaaaaaatag tttacaactg taaacaatca atgtttatag
     3301 ttgtaaacta tcaaactata tggtgactta ttgcttaaaa taggttgaaa tctcactcaa
     3361 tttttggtgg aaaaacaaaa taaattctaa tcgattttgt tgaaaaagtc ataaaaaaac
     3421 aaatgtgatt ttaacaaaat ttcaaaaaaa tcgtttgaaa tttcgtttat tttttttgag
     3481 aattttgatc atcgtagggt cattgtagaa tcattatgag atcatcgtgg gatcatcgaa
     3541 taaagtatgg gaaaggcttg cggtgcttgg tattaagggc attctccgcc tcccaggttc
     3601 ttgaccattt tctctcaacg tggtaaaaat cccacttata agagaaaatg gtcaagaacc
     3661 taaacggtcg gcgaagaaaa cataccaagc accacaagca aaaaaagtta gagtaacttc
     3721 caaaaaagaa aaatttttta gaacaacttt tgaaaattta attttgattt atatccgttc
     3781 aatttaaaca tttgataaac tgaaggctag tatttaaaat gtgtatacaa gtggtagcgt
     3841 taaaaaaagt ggaggtataa cctatgttaa aaaaaactgt gttggtactg ggttctattt
     3901 ctctaggaat attaggtttt agccccatgc tattatccgt aattggaaaa aacaattcca
     3961 acggagaact taaattaaat tcaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     4021 aagagacgga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     4081 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     4141 atgaaaaata acaccccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     4201 gcaactgcag aaaatcaagc cttaaaaaga gcttttgact tagaccaatc gaaaatcaca
     4261 aagtgagccc ctgtatccct tggtagagag gctacttggc atagtgtaca ttatgtctac
     4321 taacctttaa aataacacgt tttataaaat aaaccctctt gacaatattt aattttctga
     4381 ttattctaaa tatatacaga aagcgctttc ctaggatttt atatctaagg aggaagtaac
     4441 aatgaaaaat aaaat
//
LOCUS       DAZUMM010000131         4210 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000131 DAZUMM010000000
VERSION     DAZUMM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.137"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            189..1490
                     /locus_tag="ACG8E6_002577"
     CDS             189..1490
                     /locus_tag="ACG8E6_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5062935.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1517..1777
                     /locus_tag="ACG8E6_002578"
     CDS             1517..1777
                     /locus_tag="ACG8E6_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS5062936.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2776..4011)
                     /locus_tag="ACG8E6_002579"
     CDS             complement(2776..4011)
                     /locus_tag="ACG8E6_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5062937.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1208 a          671 c          867 g         1464 t
ORIGIN      
        1 gttaattatc gaattttttt gttgctccta tgatttagaa aacttatttt caggattctt
       61 aaaaataaat ttgattattt aagagaatat attgaaataa aatttcttaa ttatataatt
      121 agttatgaac attctttgcg ttgtcatgca atgaatggtt catattttat tgaaaaggag
      181 tggttttagt ggaaaagctg atgaaatggg tcgaagagaa acttgtccca ccaatggcaa
      241 aaattggtac acagcgtcat ttattagcaa ttcgtaatgg ggttgtttca acattgtcat
      301 tgattttgat tgggacattt tttatggtct ttatcaattt accgtttcct ggatggaatg
      361 aatttattgc cccttattcg gcaacgattg ttttaccctt taggatgacg atgggattga
      421 tggctattta tgcaactttt gttatgggtt cggatctagc taaatcatat ggattagata
      481 gtgtgacagg tggtatatta tctttaggaa cgttctttat gttacaagtt cctgtaaacg
      541 ttttaactcc tgaagaggca cctcttggct gggtcttacc tatgagttct ttgggagcat
      601 ctgggatgtt tgcggggatc ttatctatga tttttgcagt ggaagtatat cgtttcttta
      661 aaaatcgaaa tattactatc aaaatgccag aacaagtgcc acctgctgtt gcccgtagtt
      721 ttgaggcatt gattccaggt gcagtcattt taacaattac ttggctgatt cgaagcgtcg
      781 cgggctttga tgtaaacgct gccttattat cgttattcga gcctttaacc aatattttag
      841 ggaataattt gcttggtgtt ttactaccaa tgtttttgat ccatttgttg tggtcatttg
      901 gtatccatgg catgagtatt gtaggggctg ttgttcgtcc aatgtggctg atcatgttgg
      961 atgagaatgc aaaggcattg gcagatggta cagctgccac ccaattaccc tatatcgctc
     1021 ctgaacagtt ttatcaatgg acggtaacaa ttggtggtgc aggagcaacg atcgtggtat
     1081 ctgttttgtt tatattcttc tgtaaatcaa aattcttgaa agaggttggt cgtttttcta
     1141 ttattcctgg tatcttcaat atcaatgagc ccataatttt tggagctcct atgatgttga
     1201 atccttacat gttcatcccg ttcaacttag ttcctttagt tttgactatt atcagttatt
     1261 ttgcagtcaa attagaaatg gtcaatagat ttactgtttt gccaacatgg acattaccag
     1321 ctccaatcgg cggttatctt tcagcaggaa atgactggcg tgttgttatc ttgatcatga
     1381 tcaacacatt gatagcattt attatctatt atccatttgt aaaagcttat gataaaaaga
     1441 tgctgtcaga tgaacaaagt cgtggagaaa acgaagtcca gactgtctaa gtttggtttt
     1501 tagaaggtgg gaaacaatgt attggctgat tttatttttt gtttttatct ttttattaac
     1561 ggctagtcac cttatattga atatgctagc tacatatcat attcaaatca atcgatggat
     1621 ttgggcactg gcatcatttt tgatcgtgat tcttccaaag atcattgtac cgcatatgaa
     1681 tgttttgttt tcatggggga catatgtact ttgtggaatt tttgctatca attttatgat
     1741 cgagcagcat agatggtttg tcacatcgaa gctttaaatg ttctaaaagt ggttgtaaca
     1801 gaagtggtta gcttcaagaa ataagaagaa atttacgaaa attgcttctt aaatttttgt
     1861 gaaatgttgg cttatttttc gtttggttga acttattacc gcagatgcaa gcttttgacc
     1921 accgtttatt cggaatatgg gactgtgaca aggcttttgt cacagccctt ttttacataa
     1981 agattattat tgttttaact gttttggcag aaagaaggag gaaaaaatgg aaattttatc
     2041 agcaatggat ttagaattag tagatctact accaaagtga tcaagtcaaa tatagatttg
     2101 ggagcttaca accatatagt aggggctgca gtatggtgta tgaatggtaa tatgtatgat
     2161 gtgagtgttt attcaaatca tgaaacgtgt atagaaataa ttgtgctggg aatagcaatc
     2221 agtcatggtg aacgtgactt ttagtgtatc gttgctgacg acatagaaca ttctgacatg
     2281 atctactcaa tatgtgggga ttgctggcag tttatatagg agtatgcatc ggattgcgag
     2341 atcattgtct caacagaaga gggaggttga ttatcaaaga taaagtggta aatcctacat
     2401 ttgatttagt aattgttgag gggactagcg gcacaaatct ctacaaagcg atgcttctag
     2461 atctatatga cacattttaa gaaaatgtat ttgcctaatt attatggtct ttcttttgaa
     2521 gaaacgttga gatgatccaa ccagagaaag gtgtcaaaga atttgactaa gaataaatgg
     2581 cagacgctac ctatgctcat ttggatagcg tcagatcttt tattttaagg atgattttgt
     2641 gttttttaga caccgtaatt taagtaactc taacttttca atccactatt tacttttttt
     2701 gtattttcac tgtctgggtc tcgaatagcg aaattactga tgagtctcta ttaaaaaagc
     2761 tttttctact tgaaactata ttgggtggtt gctcaggaag gtttcgattt cttgaattgc
     2821 ttgttgtgga ccatttattg gaaaaacggc ttgcagcgtg cgtgcctgct gacgaatctt
     2881 gggtgaagaa acttcgcgca gagcaatctg cagttttctt tcagtcactg gttttgtgag
     2941 ggcgattgcg tttccgtatt ttacacaata gtcaatatta cagctttgct caaattgcat
     3001 ccccacaccg ataaacggtt tgccggattc acaggcagtt tggacggtcc cttccccgcc
     3061 gtggataaaa gaaaaatcaa tgaattcatg caaaagatga ctagacagat attctgttat
     3121 taaaagatct tctgagtcag attgaatgtc atcaagcaga tagtcaatag gtgcaataac
     3181 cgctagatcc ggttgttttt gtaagaagaa caaaattttg cgtaacatac gcggattgcc
     3241 tgaactaccc attgcgaaat agataatacg ttttctttct tgttgctttc gctgtataaa
     3301 agttagcact tcttcaggca aggaagtact gagcttagca aaaattggac caacgattcg
     3361 ataattttcc ggcagctgtt cttcttccaa aaatagttta ggcgttgtga tgagatttag
     3421 atcagcatcc atcatttgta aacttgtttt tggtaaagga agcatttctt tttgtgcagc
     3481 gtgtttgaag gcattgggga tccatcgggt atttactata cgcttttttg tccagtgcca
     3541 aaggggattg cttaaagggc cgaagaaacg taactcgtgg ggcatcagcg catgctctga
     3601 ttgaaaaaaa ggttcactca aagcataggg tttaacatat actaaaggaa tctttttatt
     3661 gcgggccgat aaaaaaattg tcacattcga acctgtcaca atcagtcgag gctcaaactt
     3721 agcaatcaga ctctgttcac tagtgatacg agaggcaacc atttctttgg taaaagggtt
     3781 tttaagtgta cgcaactggt caaattttat gatttgttct tcttgttttt tcgtaaggat
     3841 cggcgctagt aaatgatact caaagccact ttctgtgatc aaaggagcaa aagttttaga
     3901 aaagccaaag aatacacagt aataacggtc tttcatagct ttggcaacct caatcattcg
     3961 tgttgtctca gctaaattga atactgcagg agcaaaaaga attgtcttca tgaaatcctc
     4021 cttattggaa tgagtctatc ccttcgtttc ttactagtat actaaaaaac acagcgttcg
     4081 agtagcctat gtatattttg aatattaaac tgtctctcgc tgttttcaca acttctaaat
     4141 ttaaattttg taatcaagtt gaaactccaa ttttcaaaaa aacacgcaac aattttttga
     4201 tcgatttata
//
LOCUS       DAZUMM010000132         3935 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000132 DAZUMM010000000
VERSION     DAZUMM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.138"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..1499
                     /locus_tag="ACG8E6_002580"
     CDS             54..1499
                     /locus_tag="ACG8E6_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HFS5062938.1"
                     /translation="MLGVILMMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFD
                     FIYPIVESTYSTHGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYS
                     FEDPIPHFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANA
                     NKHKFTKEMTYGQAKAYQDELEDEINKERIVVGKRPFTWDTESELSLQKISHSDPESG
                     YYVKGEREKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRV
                     VADAGYKTPKFVHFLSHLKLYPILPYTVPHGVKGMFRKKDFVYDAYFDCYLCPNNQPL
                     LFSTITRDGYRTYKSNPKFCRSCPLLKNCTLSTKHQKIVTRHVWADLMDELEHQRHTA
                     LNREIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLW
                     KAKKPMAFYLKFLQRLTKRSWQLSHSFLSTV"
     gene            complement(1543..1788)
                     /locus_tag="ACG8E6_002581"
     CDS             complement(1543..1788)
                     /locus_tag="ACG8E6_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062939.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1805..2656)
                     /locus_tag="ACG8E6_002582"
     CDS             complement(1805..2656)
                     /locus_tag="ACG8E6_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS5062940.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2822..3826
                     /locus_tag="ACG8E6_002583"
     CDS             2822..3826
                     /locus_tag="ACG8E6_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFS5062941.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1287 a          666 c          759 g         1223 t
ORIGIN      
        1 taaattggga gtgttgtttg cagttttttt gttttatact cattgtataa actattctgg
       61 gagtgattct aatgatgaga aaacaatcga ttgaaggtag aaatcaattt gctatgttaa
      121 caattgatga tcttgtacct aaagatcact tagttcgaaa gattgatgct gcgattcagt
      181 ttgatttcat ttatcccatt gtagaatcta cgtattcgac ccatggtcgt cccagtattg
      241 atccagtggt cttaattaaa ttagtattta tccaatattt atttggtatc cgttcgatga
      301 gacagactat taaagacgtt gaaacaaatg ttgcttatcg ttggttttta ggttattcat
      361 tcgaggatcc tatcccgcat ttctctactt ttggtaaaaa ctatgttcga agattcagag
      421 aaacaacttt atttgaagac attttctctc atattctaga acaagctgta aaagctggct
      481 tcgttacgga agacaacctt tatattgatt ctactcatat taaggcaaat gccaataaac
      541 ataagttcac aaaagaaatg acctacggtc aagctaaagc ttatcaagat gaattagaag
      601 atgaaataaa taaagaacgt attgttgtag gaaaacgtcc ttttacatgg gacacagaaa
      661 gtgagttgag tcttcaaaaa ataagtcatt cagatccaga aagtggttac tatgttaaag
      721 gtgaacgaga aaaacaattt gcttattccg cgcatacttc ctgtgaagat aatggtttta
      781 tcttatctac attaatcaca ccaggaaatg tacatgacag ccaagtcgca atcgatctgg
      841 tcaaacagtc gaaaagagct tttccaaata taaagcgtgt tgtcgctgat gcaggatata
      901 agacgcctaa atttgttcat tttctgtcac acttgaaact ctacccgatt cttccatata
      961 ctgtacctca tggagtaaaa ggaatgtttc gtaaaaaaga ctttgtttat gatgcctatt
     1021 ttgactgcta tttatgtccc aataatcaac cgttgttatt ttcaactatc actcgtgatg
     1081 gttatcgaac ctataaatca aatccaaaat tctgtaggag ttgtccatta cttaaaaact
     1141 gtactttgag tacaaaacat caaaaaatag taactcgaca tgtttgggcg gatcttatgg
     1201 atgaacttga acaccaacgc catacggcgt tgaatagaga aatttataaa aagagaaaac
     1261 aaaccattga acgaatattt gcagatgcaa aagaaaagca tggtatgcgt tggacgaaat
     1321 atcgaggtct cgaaaaagtc gccacgcaca cgatgcttac gtttgctgcc atgaatttaa
     1381 aaaaactagc cacatggcta tggaaagcaa aaaagcctat ggctttttat ttaaaatttc
     1441 tacaaagact aacaaaaagg agttggcaat taagccactc ctttttgtct acagtctgaa
     1501 tggaacggat tatgtatata tacataatcc gttccattct ttctattttt tcggtatcaa
     1561 ggcaaaaaca ggtagttcag gtgaataaat gacttcatgt tcgtgccagg gtttgaccat
     1621 tagcgaatga tcgaaccgtt ttttcagttc ttcgatgatt tgatcatgtg tccagtttct
     1681 tgcaggaaaa tgttggattt tctctgcatc gatcaaaaag acaaaatcgg aatgaaaagt
     1741 ctgggtaact tcttccgaga gagaattcaa gcgttcgatt ggatccataa aatcaccttt
     1801 cgacttaaaa ttgggtagta tctgggtctt ttgctttgcc gactacacca tctaactgat
     1861 cgatttgttt catttcttct tctgttaatt caaaatcaaa taattcagta ttttctttga
     1921 ttctgcttgg tgtcactgac ttaggaagag gaaggaattc gtgttgcagc gaccaacgca
     1981 aagcaacttg tgcaatcgtc ttgtcatgtg cttcagcgat ttgtttcatt tcaggcacat
     2041 cgaagatttt accagtgcct aatggactat atgcttcgag gatcatatca tgtttttttg
     2101 catactcaac tacagttgtc tgtaattcgc ctggtgcaag gaaaacttga ttgaccatcg
     2161 gcataatttt tgctgttttt gctagtgtat ctagatggtg gggtaagaaa ttgctcacac
     2221 cgattgcttt gattttacct gcttcataca gttcttcaaa tgctcgccat gtatctgcat
     2281 ttgcttgttc ccaattatca cgaaaagcaa cagggtttgg ccagtggatc aaaaacagat
     2341 ctaaataatc tgtttggagc ttcttcaatg attcttcaaa agaagacatc acaagttcat
     2401 agctgtggtt tgcattccat aattttgttg tcaagaatat ctcttctcta ggaattccac
     2461 tttccttaat cgcttggccg acgctttctt cattcttgta tccttgggca gtatcgatat
     2521 ggcgataacc tgcagccagc gcttctttaa cagaggaaac agcgacatcc ccatcaggtg
     2581 tttgccatgt gccgaatcct actaccggga tttcgtaacc atttgataga cgataagtgc
     2641 ttgttaatga attcatactt tcagctcctt ttttattcgt tcaactataa gtgtagcgca
     2701 tttttcgaaa aaaagaatcc attttgtttc tactcttgaa atttttgttt aaaagaaaat
     2761 gattttgatt ctggtatttt tgattttttt tccgtaaact aagacagaag ggaatgagaa
     2821 aatggatatc tatttaaaaa aagcaattct gcatattatt gacagagaaa ctggttctcc
     2881 agtcttttca caaaaagagc tggatctgac aaaagaatat attcgtgatt ttcttcaaaa
     2941 gaagatccaa aaaatctctt ctgctcaaac aaaaactgga caactagcag aagaaagcat
     3001 gatcgttgaa tcacttaaaa tcgctcaaga aaattttgtg gaagcaagtg aagcaatcgt
     3061 tcagcggtgg ttcgatatct accaagaaag tgaagaagca cctagtgcag atgtatttgt
     3121 tgtcttgtat gaattagata cagtgatgta cttatcttta ttgaaagtca attatcgtga
     3181 ggcttacact cactttgttg aagcagacga agcagggatc gacaaccgct tgattttgaa
     3241 tcgagcaatt ctaggggcga aaacacaaaa agcagatgaa gcattcgctg tgaatttgac
     3301 tgattggacc tacgaattaa ttgaaaaaaa atatgagttt tctggcaaaa aagagcttta
     3361 tttttctgga agagtgatcg aaagtgttcc tgcaccttct ttagaagaaa atgtaaaagt
     3421 gatccaaaaa gtagccaagc atcttggaaa aaaatttgag acagaagaat ttgatatcat
     3481 ggctgatttg aaagaagctg tctatgatac gatagaagaa aaaggtcggt tggatcatga
     3541 gatgattgcg gaaaaagtct tcaaggaaaa catcacggca aaaatggctt ttaaagaaga
     3601 agtacaagag caaggatttg tcccagaagc acctcctgta aaagaagtac aagaaatttc
     3661 tgagaaaaaa tttggaaaac aaaaattcaa gctatcaaac gggatcgaac tgatcgtacc
     3721 agtagatgta tatcgcaacc cagatttgat tgaatttgtg aataatccgg atggtacgat
     3781 atccgtgatg atcaaaaatg tggatgaagt attgaatcgt ttataaaaat gactagaata
     3841 ttaggtgttc aagtaccagt gatcagttaa atgaattgtg tcaagagaag gatcggaaaa
     3901 gacgaatata acaaaaaaag agcgtgagac aaaag
//
LOCUS       DAZUMM010000133         3907 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000133 DAZUMM010000000
VERSION     DAZUMM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.139"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..606)
                     /locus_tag="ACG8E6_002584"
     CDS             complement(64..606)
                     /locus_tag="ACG8E6_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFS5062942.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            741..1130
                     /locus_tag="ACG8E6_002585"
     CDS             741..1130
                     /locus_tag="ACG8E6_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062943.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1134..1898
                     /locus_tag="ACG8E6_002586"
     CDS             1134..1898
                     /locus_tag="ACG8E6_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5062944.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2027..2974
                     /locus_tag="ACG8E6_002587"
     CDS             2027..2974
                     /locus_tag="ACG8E6_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5062945.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3104..3844
                     /locus_tag="ACG8E6_002588"
     CDS             3104..3844
                     /locus_tag="ACG8E6_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062946.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1294 a          667 c          799 g         1147 t
ORIGIN      
        1 aaataaaaac tgtctaacat ttaaaattta atttagatat tagacagttt ttaagtttgt
       61 gagttatttt ccttgtcgtt ttctcacttt tttagtccag aaaccacctg aaatatattt
      121 tggctcataa gaaatgataa aagcacgctc ttcgatttct ttgatcaggc gatacagttc
      181 acgttcgctt tttctaggag aaagtatttc gagaactagc cgctcacctt ccagccccat
      241 tgcagaagtc tgtgtcacac cataaccgtg ttctctcagt atacccggca aacttttatc
      301 ttctgcgcta tttgctggca aaatcgttgt gaccatgatg tatcctaatg ccaatttgtc
      361 ttcaatcaaa attccgacac tgatacctac tgcgtaacct aacgcataga caaataaatt
      421 cagtggatta tctaaacgat ctaaaacaag ggataagccg acgatataaa tcgtgatctc
      481 gaccatactg acaagtggag caatcagtcg atagcctttc attgtcaaca tgaaacgtaa
      541 cgtgttcaga gtgatataag caaaattaat gaggaagatc atccccaaca tttttggatc
      601 aatcatttgt aaaactcctt tcatttatat aacaacaata tcaaaaaaaa tcgtaagtga
      661 agaaaaattg aaactttata catacaaaaa aatgataaaa tgattataat agaattataa
      721 agataggtta ggtgattaaa atgaaaacgg tcaatcgtgt gttttgtgcc agccacgtgc
      781 taagtctcct tctcagtata tacttaggtg tctattcgat tcaacagcca attgaggtag
      841 agaacattgt ttttcagatg tcgctgacag atggtttaat tttaagcgtt tttttctttt
      901 tgatactgtt tattggtaat attttcggta gcgttgccag ttttttgaat ttttctattt
      961 atccactcct ttcgctagca ttaggagtgg tgggactttg ttctctgtgc ttatttcccg
     1021 agccattcag tcctgcattt atattatttg gtacactgaa cctgttccag acaactgttg
     1081 gagcatggtt gctatggcga tcaggaaatt taatgaaaat aggtgagtaa caattgaatc
     1141 caattatatt gaaacgttct gtccatcata ttccagtgtt agaagttgtt ctgaaagaat
     1201 taagagagga acgtattccc gttgtcatct attatcacgg atggcagaca agtaaggagt
     1261 tagttttgac gcaaggaaga aaaattgcgc agcagggcat tcgtgtgatc ctgccagatg
     1321 cgatgaatca tggggagcgc aaacagccgg tttcttctat tccatcctat acattttggg
     1381 atagtatcta cggaaatctg tttgaatttg acacacttat caagcattta gaaaaaagag
     1441 gtcttctaca tgatcagctt gcagttggcg gggtttcgat ggggggaatg acaacatgcg
     1501 ccttgttggc taaacatcct catatagtag cgggcgtgtg tttgatgggt tcccctgcac
     1561 ctttggaata tggaaaagaa atcgtaaggc gcgcatcgga atataattat gtcgtaccaa
     1621 aagactattt tgatttgatt tcatgggtta agagctatga tttgtcattg cagccggaaa
     1681 agttagctgg acgtcctctg ttcttttggc atggacaagc tgacgaaaaa atcccttata
     1741 tacaaacgga acgatttgta gaaaaaaatc ggcataagtc ttacggaaaa gaaattgttt
     1801 ttcgttcctc aaaaacacag cgacatatgg tacaaatacc actaatggaa gaagcttcca
     1861 cttttttagc aaaaaatttg ataaaaaagt ataaataatt gaaacggaaa atcactcatg
     1921 acgatagagg attttccgtt ttttattttg ggctataata aactctgtta ggaaaatttg
     1981 gtataattga tagtaagaat gacaacatgt tagaaaggaa gagagcatgc ccactttagc
     2041 agatgttgca aaacgtgcca atgtttcaaa aatgacggtc tctcgagtca tcaatcatcc
     2101 ggaacaggtg actgacgaat tgaaagagtt agttttctca gcaatggctg aactggatta
     2161 ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg tcgcagatca tcaaattatt
     2221 tatactggaa gaaatcgaca caactgagcc ctattacatg aatttgctga tgggaatcgc
     2281 taaacggatc ggtaaagcgc attattctct ccaattagtt acaaatgatg ccttcgacgt
     2341 cggttcttgt gatggataca tcatcacagg tgtcagaaaa aaagacttcg attggatcga
     2401 ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct gaaggatatg attatgtaga
     2461 cagcgataat gaatatggta cagcaaaagc aacgcaatat gccttagaaa gagattatca
     2521 aacgattatt tatatcggta tcgaagtgga cgagcctttt gaaatttcaa gggaaactgg
     2581 ttatttatct gtgatgaagg aagttgatct tcctagtaaa atcttgcggt ttccaaatca
     2641 ttcgacagaa gtagaaagat atatgacaga aaactgggca gattttacag ataaaacttg
     2701 ttttgtatgt agttcagatc ggctagcact aggagcagta agagggatta taaatgctgg
     2761 aggacacttg cccgaagaat ttggtgtgat tggatttgat ggggtatttt tagaccaaat
     2821 cagttcacct aaattgacaa cgatcaagca agatattata cgtatgggtg aagtatgtgg
     2881 agaaatgttg ttaaaaaaaa tagaagaaaa aggggcgagt caaggttatc gtcgtttttt
     2941 tcctgaacta gttatcagag agacaacgaa ttaagtctta ttactatata tatatttatg
     3001 aaattcatcg ctattttctt aaaataatca gcgcattgct actgaaaaca atgaatctgt
     3061 gctattattg agaaaagttc catttttgag aaggatggtt tgaatgaaag aaatcagacg
     3121 aaaacatttc cgttttcctg cgtacgatga tgagttaggc gtaaaattaa ccaacgacaa
     3181 gaagcgagta ttatttgacg gacaagaaga tcttttgact gaacagaatc agtccccgtt
     3241 tgaacagatg aaagaatttc atttcacgaa cacgaacacc gagaaaatcc gtcgcaagcg
     3301 tcgtgcaaca aatggattga agtcagctcc gcgtaaggaa aatttggaaa agcataaagg
     3361 aaaattaccg gattataccg gccattacca atcagaaatc acatcgacag gacgaaaaaa
     3421 actttttggt gattcctctc gttctgtcag acaaagtgaa acaaggacag agtccaagca
     3481 tactacacga aacgcatctg ttgacacgaa ggtgcgggag cgttcttatt ttgttcctaa
     3541 atatgtaccc gcatcgatta tcccagaccc tagagaaagc aaaatttctg aggatcattt
     3601 aatcgaatcg atgaaaaaaa cggatgactc ttatctgttg ttcgatacag aaccgacgcc
     3661 gtatcaagaa aaaagaaatg gtgagccgtc tgtacgaaca ttcaatcgaa caaaaacgtt
     3721 agaggaaaca aagcagtcag aagaaaaaaa atcgaaaaaa agccatggta tcttagaacg
     3781 ctcattacaa ggattgatcg atgatcaagg aaactctttg ggagaaaata gctattttaa
     3841 ataagtgata tacaaattta tattcggcta cagttggaaa ggaaacgctg cggcgtttcc
     3901 ttatttt
//
LOCUS       DAZUMM010000134         3785 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000134 DAZUMM010000000
VERSION     DAZUMM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.142"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..168
                     /locus_tag="ACG8E6_002589"
                     /pseudo
     CDS             1..168
                     /locus_tag="ACG8E6_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            406..642
                     /locus_tag="ACG8E6_002590"
     CDS             406..642
                     /locus_tag="ACG8E6_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062947.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="ACG8E6_002591"
     CDS             649..2601
                     /locus_tag="ACG8E6_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS5062948.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="ACG8E6_002592"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="ACG8E6_002592"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS5062949.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3445..3785
                     /locus_tag="ACG8E6_002593"
                     /pseudo
     CDS             3445..3785
                     /locus_tag="ACG8E6_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1344 a          614 c          655 g         1172 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tatta
//
LOCUS       DAZUMM010000135         3715 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000135 DAZUMM010000000
VERSION     DAZUMM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.144"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(154..327)
                     /locus_tag="ACG8E6_002594"
     CDS             complement(154..327)
                     /locus_tag="ACG8E6_002594"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062950.1"
                     /translation="MNKMKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGM
                     LIYFVFSDDGKYG"
     gene            613..1941
                     /locus_tag="ACG8E6_002595"
     CDS             613..1941
                     /locus_tag="ACG8E6_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFS5062951.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2143..2976
                     /locus_tag="ACG8E6_002596"
     CDS             2143..2976
                     /locus_tag="ACG8E6_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFS5062952.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2992..>3715
                     /locus_tag="ACG8E6_002597"
     CDS             2992..>3715
                     /locus_tag="ACG8E6_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFS5062953.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANII"
BASE COUNT         1235 a          587 c          772 g         1121 t
ORIGIN      
        1 agtcctactg ccggagtaaa taaagcttat gcaaagaaaa agaaatccaa tcacttgggt
       61 ttctttttct tttattatta tgatgaatca agctttctgt ttttgtcacg tatgttgtaa
      121 tctttacgaa agagtagagt tggctactca taatcaacca tacttcccat catcgctaaa
      181 tacgaaataa atcagcattc ctacaccaat caaagcacaa gctgcaccaa gaaaagggat
      241 gacattccca aagttatctg tattcataag tatgccacca cccacaaaca aaaagccgcc
      301 agcaatcagg tttttcattt tattcatctc ctttcttacg ttatagtatt tccttctttc
      361 aatgagtatt ttgaaagcta tcttattgta tgatgttctt ctaggcctct tctttattat
      421 acaggccatt ttcgttatct catagaaata ttccttttta tctgaaaact attgtatacc
      481 gtatgacatt tttcttcatg aagttgtaag tatattctca ttcaaatgaa aatttcatca
      541 tagaagagat tgtatttcta aattcttaag agtaaaatac gaagtataac ttggatttgg
      601 aaggatgatt ggatgaaagc tattacgcct gatatgacaa caaaatttaa caaacagttt
      661 caagcaaata tcaaacagca ggcattgcag cgggcagttg tgaagaacgg aattactgct
      721 tctgctgaaa atgtttcagc acatgtggag aatacaccgg tattttctat agatttggct
      781 acggggaaag tggctaatca aatgcaatcg gggcgttgct ggatgtttgc tgcactgaac
      841 acattccgtc ataagatgct gaatcaattc catttaaaag agtttgaatt atcacaaaat
      901 tacacttttt tctgggataa atatgaaaag gcaaattatt tctatgaaaa catcattgca
      961 acaggaaaag aggcaatcga tagccggaaa gtagctttct tactcgctac tcctcaacaa
     1021 gatggcggcc aatgggatat gatcgtttca ttattccaaa aatatggcgt tgtgcctaaa
     1081 tcggtgatgc cggaaagtgc gaatagttca aattctcgag atttgaataa ctacttgaac
     1141 aaattactgc gtaaagatgc agtagtgtta cggaaaatgg ttgctcaagg agaaacatta
     1201 gacgctattg aagaaaagaa agaagaaatg ctagaaagca tttataactt cttagctatc
     1261 tcattaggaa cacctccaaa agaattcgat tttgagtacc gcgatgaaga aaagaactat
     1321 catcttgacc gaggactgac accacaaatt ttttatgata aatacatcgg tgtaaaatta
     1381 gatgattatg ttagcgtgat caatgcacca acaaaagaca aaccattcaa tcgttcttat
     1441 acagtagaga tgctaggaaa tgtcgttggc gggaaagaag tcaaatatct gaatgttgat
     1501 atggaaactt tcaaaaaatt agcgattgct cagcttgaag aaggagaatc tgtttggttt
     1561 ggttgtgatg taggtcaatc ttctactaga actagtggca tcatggcctt agatgcttat
     1621 tcaatggatg atctatttga tacggatttt acaatgacta aagctgaacg tttagatttt
     1681 ggagaaagtc tgatgaccca tgcgatggta ttgaccggtg ttgacttagt agacgggcaa
     1741 tcaacaaaat ggaaagtcga aaatagctgg ggcgaaaaag ttggaaagaa tggcttcttt
     1801 gttatgagtg atgcctggat ggacgaatat acgtaccaaa tcgttgtaag aaaagattta
     1861 ttgacgaaag agcaattaaa tgcgttcaat gaagaaccaa ttgttctatc tccgtgggat
     1921 ccaatgggtg ctttagcata gacgagtatt tcaatgaaga gtatgtagta aagataaaaa
     1981 gagctggctt aagctagctc tttttatctt taaagtccat tccctgtttt ttatggtccc
     2041 tgtgctataa ttctaaataa aggataagga ttggaaataa tccttttatc attaaaaata
     2101 tgatgacgaa gtagaataaa attgggagta ggggggaaag tcatggatat ccaaactgtt
     2161 ttatctgatc tgaaattaaa tggtaaagtg attccagttg ttggtggcga tgttaatcag
     2221 acctatcgta taaaaaccga acatcgtgct tattttttaa aaatccatcc gaatgtaaaa
     2281 aaaggatttt ttgaagcaga agtagacgga ttaaaagaac tgtcagcatt cgtacgtgta
     2341 ccggatacgt atatgctagg tgaaacaagt gaaggcgctt atttgttaat ggaatggatc
     2401 gagccgggaa aaggggatca acgagatctt gctgcagctt tagctaatct ccatcagcaa
     2461 acagcgccac aatttggttt tcgtaaagat aactatttgg gtacacttgt tcagaaaaat
     2521 tcttttgaag aagattggtg gacttttttc tttaaagatc ggcttgaatc ccagatttct
     2581 ttggcagaag aaacgaatcg atggaatgta cagcgtcaag aaaagtatct gcgatttaaa
     2641 gaacgtgtat tgaaatcggt tgaaccaaaa aagatcactc cacgcctctt gcacggagac
     2701 ctgtggagtg gtaatgtgtt ctttgaccag caagggcatc cagtttttgt cgacccagct
     2761 gtttcttatg ggaatcggga acaagatatt gcaatgagcc agttattcgg tggtttccga
     2821 ccggaatttt tagatgctta tcaaactata tttccattag aaaagggatg gaaagaccga
     2881 ctgccgattt accagttata ttatcttttg gcacatttga atatgtttgg agaaagttac
     2941 ggttcccaag tagatcaatt actagaaaat ttttaagagg agggcgtgtt tttggcaaaa
     3001 aaatatacca aaaagcatga agtttcatat tatgagtgtg acatcaacca aacgatgaca
     3061 tttccttcga tgttaggagt agttatcaaa acgtctgaag atcaaagcga ccagttggga
     3121 agaggcagtg aattcgtcaa tagctttgga cttacttggg tcatcacgaa ttattccatg
     3181 gagattaatc gtctccctag agtcggtgaa gtaatctcag taacgactca agccatggag
     3241 tacaataagt ttttctgtta tcggaacttc tggatccatg atgaagatgg gaaagaatta
     3301 gtcaaaatag aatctgtttt cgtattgatg gattttgtga atcgaaaaat gagcagtgtt
     3361 aatgaagaga tcattgctcc ttttgagagt gagaagatca agaaaatcaa gcgtcaggaa
     3421 aaaattgaaa agattgattc gggcttcatg ttgccatatc gtgttcgttt ttatgatatc
     3481 gatagtaatc agcatgttaa taatgcgatg tattttaatt ggatcattga cgtattagga
     3541 tatgactttt tgactacgca tgttccagaa aaagtaatta ttcgatttga taaagaagta
     3601 gagtacggga atgagatcga aagccatttt gaacaaatcc ctatcgaaaa tggtgtcaaa
     3661 acaaaacatg aaatccgaat gcaagaacag ctgtattgtg aagcaaatat tatat
//
LOCUS       DAZUMM010000136         3395 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000136 DAZUMM010000000
VERSION     DAZUMM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.146"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..456)
                     /locus_tag="ACG8E6_002598"
     CDS             complement(<1..456)
                     /locus_tag="ACG8E6_002598"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5062954.1"
                     /translation="MKSQQFFFKYVNIDASNLKGELIIKPKYLEIKQNLKQQIVTGQF
                     HNGDRFYSESEIIKKYNVSSITAIRALKELVAEGYIVRYQGIGTFVSRARKEKIVKFS
                     DIEIFSFDNDKTHVLSIKRENKKQILDTLKLSTDSYYYQIERIREANEIP"
     gene            510..2288
                     /locus_tag="ACG8E6_002599"
     CDS             510..2288
                     /locus_tag="ACG8E6_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFS5062955.1"
                     /translation="MTTFEIKEEFLLKGKTFKILSGAIHYFRIPPCDWEHSLYNLKAL
                     GFNTVETYVPWNLHEPQKGEFHFEGILDLERFLTIAQDLGLYAIVRPSPYICAEWEFG
                     GFPSWLLREPIHIRRNEIAYLEHVADYYDVLMKRIVPHQLNNGGNILMIQIENEYGSF
                     GEEKEYLRAIRDLMIKRGVTVPFFTSDGPWRATLRAGSMIEDDILVTGNFGSKAKDNF
                     NSMKQFFKEYDKNWPLMCMEFWDGWFNRWKEPIIQRDPQELAEAVKEVLEQGSINLYM
                     FHGGTNFGFMNGCSARGVIDLPQITSYDYGAPLDEQGNPTEKYYALRKMIHDNYPEIK
                     QLDPVIKPTIEKKKISLTNKVSLFATLDTISQPVQSKYPKTMEELGQNTGYLLYRTMI
                     ERDAEEERLRVIDGRDRSQLFLNQELQATQYQTEIGEDIQVSMTEEINQVDILIENMG
                     RVNYGHKLFAETQKKGIRTGVMADLHFITNWQQYSLPLENCKTVDYSKGWQPQHPSFY
                     RYEFLLEDIGDCFIDLRKFGKGIVFVNQTNIGRFWEVGPTLSLYISSGFLKVGKNEIV
                     IFETEGTYEPEINLLKAPLYKEMKEG"
     gene            2292..2771
                     /locus_tag="ACG8E6_002600"
     CDS             2292..2771
                     /locus_tag="ACG8E6_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS5062956.1"
                     /translation="MSIIGVRIDGRLIHGQVANLWTTKLNISRIMVVDDEVAQNDIEK
                     SGLKLATPAGVKLSILPIEKAAQNILAGKYDSQRLLIIARKPDRLLKLVELGVPIKEI
                     NVGNMSQTSETRSITKSINVVDQDVETFNQLNEKGVHLIAQMVPSDKADDFMSLLNK"
     gene            2794..3201
                     /locus_tag="ACG8E6_002601"
     CDS             2794..3201
                     /locus_tag="ACG8E6_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5062957.1"
                     /translation="MAGHLVLISHGRFCEELKRSVDMIMGVQKDIYTVGLLPEESAKD
                     FRVNFEKTINGLKKVTVLADLMGGTPCNVVSQLILEGKEIELYAGMNMPMVIGFVNNQ
                     FLGEDSSLVNKGRENIIHVNEYLEVNPIDLEDE"
     gene            3194..>3395
                     /locus_tag="ACG8E6_002602"
     CDS             3194..>3395
                     /locus_tag="ACG8E6_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062958.1"
                     /translation="MNECGGIYEYKKIQIYFIATCCLISLSMLTKVAHAESNPIGNCY
                     YVSSQRGSDTNSGLSISESFKSL"
BASE COUNT         1179 a          437 c          692 g         1087 t
ORIGIN      
        1 aggtatttca ttagcttctc taattcgttc gatttgataa taatatgaat cagttgataa
       61 ttttaatgta tctaatattt gttttttatt ttcgcgttta atcgaaagaa cgtgtgtttt
      121 atcattgtca aatgaaaata tttctatatc agaaaatttt actatttttt cttttcttgc
      181 acgtgaaacg aaagttccta ttccttgata acgtacgata tacccctcgg caactaattc
      241 ttttaacgca cgaattgctg ttatagaact aacattatat tttttgataa tttctgattc
      301 tgaatagaat cggtcgccat tatgaaattg accggttaca atttgttgtt ttagattctg
      361 tttgatttct agatatttcg gttttattat aagctccccc tttaaattac tcgcatctat
      421 attaacatac ttaaaaaaaa attgttgact tttaatacaa aagcattaat atactaagta
      481 tattaacaca ttgaggtgaa aagtaacaga tgactacatt tgaaataaag gaagaatttt
      541 tattaaaggg aaaaacattt aaaatactat ctggtgcaat tcattatttt cggattcccc
      601 catgtgattg ggaacattct ttgtacaatt taaaagcact aggatttaat acagtagaga
      661 catatgttcc ttggaattta catgagcctc aaaaaggaga gtttcatttt gaagggatat
      721 tggatttaga gagatttttg acaatcgctc aagacttagg attatatgct attgtgcggc
      781 catcgcctta tatttgtgct gaatgggaat ttggagggtt tccgtcatgg ctactccgtg
      841 aaccgattca tatccgaagg aatgaaatcg cttatttgga gcatgtagcg gattattatg
      901 atgttttgat gaaaaggatt gttccccatc aacttaataa tgggggaaat atattgatga
      961 ttcaaattga aaatgagtat ggatcttttg gggaagaaaa ggaatatttg cgtgcgataa
     1021 gagatttgat gattaagcga ggagtgacag ttcctttttt cacttcagat ggaccgtgga
     1081 gggctacttt acgagcggga agcatgattg aagatgatat tttggttact ggtaattttg
     1141 gttctaaagc aaaagacaac ttcaactcta tgaagcagtt ttttaaagaa tatgataaaa
     1201 attggccact gatgtgtatg gaattttggg atggttggtt taatcgttgg aaagaaccaa
     1261 ttattcaacg agatccacag gaactcgctg aagcggtaaa agaggtatta gaacaaggaa
     1321 gcattaattt atatatgttt catggaggaa cgaattttgg gtttatgaat ggttgttcag
     1381 caagaggagt gattgattta ccgcaaatta cttcctatga ttatggtgca ccattagatg
     1441 agcaggggaa tccaacagaa aaatattatg cattgcggaa aatgattcat gataattatc
     1501 cagagattaa gcagctagat cctgtcatta aacccacgat tgaaaagaaa aagatttctt
     1561 tgacgaataa ggtaagtctt ttcgcgactt tagataccat tagtcaaccg gttcaatcaa
     1621 agtatccaaa gacaatggaa gagttgggac aaaatacagg ttatctactt taccgaacaa
     1681 tgatagagag ggatgcagag gaagaacgat tacgggtaat tgatggcaga gatcgcagcc
     1741 aattattttt gaatcaagaa ttacaagcta cccaatatca aacagaaata ggtgaagata
     1801 tacaagttag tatgacagaa gaaatcaatc aagtggatat tttgattgaa aatatgggac
     1861 gtgtcaatta tggacataaa ctctttgcgg aaacgcaaaa aaaaggaatt agaacgggag
     1921 taatggcaga cttacatttt attacaaatt ggcaacagta ttcattgcct ttggaaaatt
     1981 gtaaaactgt ggattattca aaaggatggc agccccaaca cccaagtttt tatcgatatg
     2041 agtttctttt agaggatatc ggtgattgtt ttattgattt aaggaaattt ggtaaaggaa
     2101 ttgtctttgt taatcagacc aatataggtc gtttttggga agtaggtcca acgctttcac
     2161 tgtacatctc tagcggcttt ctaaaagttg gtaagaatga aattgttatt tttgagacag
     2221 aaggtaccta tgaaccagaa atcaatttat taaaagcacc attatataaa gaaatgaaag
     2281 agggatgaac aatgagtatt attggagtaa gaattgatgg acgtttaatt cacggacagg
     2341 tagctaattt atggacaacc aaattgaata tttctcgaat tatggttgtg gatgatgaag
     2401 tggctcaaaa tgatattgaa aaaagcggat tgaaattggc taccccagca ggggtgaaac
     2461 taagtatttt gccgattgaa aaagcggcgc aaaacatttt agcaggaaaa tatgattcac
     2521 aacgtttact gattattgca cgaaaacctg atcgattatt aaaattggta gaattgggcg
     2581 ttccaataaa agagattaat gttggaaata tgtcacaaac ttctgagact cgatcaatta
     2641 cgaaatcaat taatgttgtc gatcaagatg tagaaacgtt caatcaatta aatgaaaaag
     2701 gtgtccattt aattgcgcaa atggttccaa gtgacaaagc agatgacttt atgtctctgt
     2761 taaataaata ataatatacg aggaggtgaa aagatggcgg ggcatttagt tttaattagt
     2821 catggacgat tttgtgaaga gttaaagaga agtgttgata tgatcatggg agtacaaaaa
     2881 gatatttata ctgtagggtt attacctgaa gagtcagcaa aagattttcg agtaaatttc
     2941 gaaaaaacaa ttaatggttt gaaaaaagtt acagttctgg ctgatctaat gggaggcact
     3001 ccttgcaatg ttgtttctca attaatttta gaaggaaaag aaatagaact atatgcagga
     3061 atgaatatgc caatggttat aggctttgtt aataatcaat ttttaggaga agatagttct
     3121 ttggtaaata aaggaagaga aaatattata catgtaaatg aatatttgga agtaaatcca
     3181 atagatttag aagatgaatg aatgtggagg aatttatgaa tataaaaaaa tccaaatata
     3241 ttttattgca acttgctgtt tgatcagttt atctatgtta acaaaagtcg ctcatgctga
     3301 aagtaacccc attggtaatt gttactatgt tagttcgcaa agagggagtg acactaattc
     3361 aggattatct atatctgagt cttttaaaag tttaa
//
LOCUS       DAZUMM010000137         3328 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000137 DAZUMM010000000
VERSION     DAZUMM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.147"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACG8E6_002603"
     CDS             complement(<1..2232)
                     /locus_tag="ACG8E6_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5062959.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACG8E6_002604"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACG8E6_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          913 a          745 c          537 g         1133 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgca
//
LOCUS       DAZUMM010000138         3295 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000138 DAZUMM010000000
VERSION     DAZUMM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.148"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(436..798)
                     /locus_tag="ACG8E6_002605"
     CDS             complement(436..798)
                     /locus_tag="ACG8E6_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS5062960.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            1021..1773
                     /locus_tag="ACG8E6_002606"
     CDS             1021..1773
                     /locus_tag="ACG8E6_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5062961.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(1913..2836)
                     /locus_tag="ACG8E6_002607"
     CDS             complement(1913..2836)
                     /locus_tag="ACG8E6_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5062962.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(2851..3123)
                     /locus_tag="ACG8E6_002608"
     CDS             complement(2851..3123)
                     /locus_tag="ACG8E6_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062963.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         1080 a          620 c          536 g         1059 t
ORIGIN      
        1 tatccgagac acgaagaaat tagcgttttc tatttttctt acttgataaa ataagtagat
       61 tttcctacta caatcgtagc cgttaagata ccgatgattg aacaaatata accaaatgtc
      121 tctacaacat aatcaatggc aaaagttttt gaagcaataa tacgtctttc cttttttgct
      181 tcttatcccc aaagaaacgg atataactta ttatgtattg aatttttttg gaaaggcgaa
      241 ttatgacaag atataccttg cgttgaaaaa actcgcggaa aaaatatcac acgtttttaa
      301 aaagatacaa agtaagaaag cagctgtcaa tagaatggaa acaaaaatgg cgagacatca
      361 atcagtctcg ccttgttttt tattgtatac attagatatg gagcaggtta ccataaagat
      421 gattattagt cgttattaaa tagaatcctt cgtatttttt tgttctatca ctacaatatc
      481 tttatttgga cgtttgaatt gtattccggc acgttctagc tcctgtttca ctcctacatg
      541 gactttgaca tcttctcgtg tcatttttaa aacgtgatga atcgcatagt cgatttcttg
      601 ccttgtgatt tttttcttcg tattgatcat cacgacttgc tttttatcat atgtgtctac
      661 caatagcagt tgtgttcgat ctaacgaaaa taatttgtaa taattaatag ccgtattccc
      721 aaaaaagaat tccactactt taggataaaa tgtcttaata tttaaattac tattcacagg
      781 tgtttcgatt agcttcatct cattcctctt ttctatatag tcgatttaga tccaatctta
      841 agttatgtta ccatctttcc tagcattaag gaatcggtta caattgaacc tttaaagaca
      901 aaaggattta ccttttatca cttaatagta taacataatt tcgtgagaaa aaaatacaat
      961 tttttcttga ccatctcgtt acgtaaagga ttatgcttac atttgtcagg aggaaaaata
     1021 atggagtaca caataaagaa aatgtcagag atatcaggag tcagtccgag aaccttgcgc
     1081 ttttatgatg agattggact gctaaaacca gctaggatca attcttcagg ttatcggatt
     1141 tatggcaaaa aagaagtcga tcgtctgcaa cacatcttat tttatcgaac aatggcattc
     1201 aagttggatg atatccaaga ggtcttagac aatccatctt ttgaccacca gaaagcttta
     1261 atcaagcatc agcaaatgtt gctggaaaaa cgggcacaaa tcgataccct cttgacaact
     1321 gtccaacaga cacttgatat gtacgaagga ggaagaaaaa tgagtgacaa agaaaaattt
     1381 gaagggttta aacaacaaaa gttgaaagaa aatgaagaat cctatggaca agagattcga
     1441 caaaaatacg gaaaaaaaaa ggttacagca tccaatgaaa aatggatgaa tatggataaa
     1501 gaaacgtatg aagaaatgca aactgttgaa aagcagatgt tatttgatct cactctttac
     1561 ttaaaagaac cagaagatga aagcttagca gaccgtatct tccaagctca taagaagtgg
     1621 ttctcttaca gctggcctga atatcagcca aaagcgcata aaggactagg aatgatgtac
     1681 gaagccgatg aacggttcac tgcatattat gacgagcgaa gtggtcatgg tgcagcaaaa
     1741 gctttaaatg aaattatcca aaaaaaggcg taatctttta catgatccaa caaaaaagta
     1801 cacgtaaaat gacctaataa caaaacagca gacacccaag ttagattttt atgctaactt
     1861 ttggtgtctg ctccattctt tcacaagttt tttcatccta ctttaaatat attcatcgtt
     1921 gaataaacgt ataaggtatt ttcactttat cctttttttc tgtaagaaac aattgaaatg
     1981 ccatttctcc gcaagcttcc agatggtggt cgatggttgg gaaaccaaga agctcgctga
     2041 tcagcaaatt atcgcgtcca atcaccactg gtgtcttatc ttgcagatag ctttgcaaga
     2101 tgcctgctgc aaccatatcc gaatttgtaa agacaccatc aactccaagc tctttaaaaa
     2161 agaagcctgc ttgttttcct ttctctgcat ctatacaatc ccaaaaaaca agtgattcat
     2221 caaaagatgg aaaaagttga tgacatagct gcaaagtgat ttttgagtta taacttagcc
     2281 tgccacttcg ccccaacgtg atgcccaatt gctttatacc ttgctgcttc aaataagtaa
     2341 gaccttctgt aattgattgt tctcgatcaa taaagacaca gcttgcctgt tttcccttga
     2401 tttcttcaca aaaaactaga ggtccatatt tttgatatgc caataaatca tctagtgtat
     2461 tggcacgaga cgtaatgatc aatccatcga aagctttagc tgcaaattct tcaagatacc
     2521 ttcgctcttt ttctttatga tagcccgtcg gtaaaagtgt cactttgtaa tcataatcaa
     2581 acgctgcttc aatgatccca ctgatcaact gatcaaaata cggatgattg gtataaggca
     2641 gaatcactcc aatgcttttc gtttttccat aacttaactg tctggcattt gcattaggga
     2701 tataatcgac ttctttcatg accgcaagta tttctttcct tttttcctca tctacatatg
     2761 gatgtccatt gattacacgt gaaatagtag aaactgaata acctgtgagc tttgcgatat
     2821 ctcgaatgtt tgccactgct gcttcgccac ctatacaaaa agattttctt gtaaatagtc
     2881 taccacagct ggaccgcatt caaaaaactg atacattttt ccttcatgcg aaaaactgat
     2941 ccgctgcccg ccccaatata tatcttttgt caaattttcg atttcattca ttggtatttc
     3001 ttttactata ttcctattca acccttgaaa actgtacaac gttaatacgg atcccttgaa
     3061 ctcgacaaac gcataaaaat aattgttcgt tgtgccatct gttatctcta cataagtatt
     3121 cattgcaagc cctccttttg atactttgag tatagtgtat gaaacgcgtc tcatagcaag
     3181 gagtttgttt tgccaaagaa aagttttatt cctctttcat tattaccaaa gtctcttatt
     3241 ttaaattgaa tacattataa tgatactgta aatatatctt tattattagt gaatg
//
LOCUS       DAZUMM010000139         3282 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000139 DAZUMM010000000
VERSION     DAZUMM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.149"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(499..1113)
                     /locus_tag="ACG8E6_002609"
     CDS             complement(499..1113)
                     /locus_tag="ACG8E6_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5062964.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1719..2096)
                     /locus_tag="ACG8E6_002610"
     CDS             complement(1719..2096)
                     /locus_tag="ACG8E6_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062965.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2396..3277)
                     /gene="galU"
                     /locus_tag="ACG8E6_002611"
     CDS             complement(2396..3277)
                     /gene="galU"
                     /locus_tag="ACG8E6_002611"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS5062966.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         1005 a          670 c          501 g         1106 t
ORIGIN      
        1 gaatttcatt gcaaaaaaaa taataaacac ttataaatat ccattatcta tccttcaatt
       61 tttgtgtatt ttatttctat tagaaaaagg tatcctgttg gacaattttg ttatatcagg
      121 taaacttcat tgcaataatg ataacaaatg tactataatt actttgtaaa gagttttggg
      181 gaagactctt tcatcaacgt gtattaagag acaagaaaga atccatatgg attctttctt
      241 gtctcttttt ccactacttt ttatttttct gacactacat attcttataa ctcatttctc
      301 ataactgtga aacaacttat aaaaataaca aactttaaac aaaacaagct taaaatcaaa
      361 gatgatccat cttaaaataa ctaaaacaaa aaaagtagtc gttgttaatc ctcattaata
      421 atcgttttct ttttttcaaa cgctccattt ttatgtggaa cattattttt ttaaggaact
      481 ttgtcaacat gtggataact attttccact tgtgtcctgg actagctgtt ttgctgtaaa
      541 atacaaaact tgctgcagca aaaagatcgt tgcaagagag gctgttccgc agatcgctgg
      601 atctagattt ggcaaagtca agactgcatc atgtaaattg gcgtggttgt ccaaaaccaa
      661 atcagcaaat tcaaataatc gcaaaccact gctatgttgc gatttgatac ttcgtgacaa
      721 atcaaatcca gtaacaatga ttaatggatg accattttct ttgatccact gagctagttc
      781 gatgattgcc gggtttcttc cttcattcga aagaagaaga aaaatgtctt ctgatttgac
      841 agaaagctga tcaataattg cctgtcctgc accttctagc gtttcataga cacctccggc
      901 tggattttca atcaatatac ttggtacaag atccggggtt tgatcccaaa attcaaatgc
      961 tacaccactt agtgtgcggc tagcatatat ctgcaatcta ccaccttgat taatttgttt
     1021 cgcgatccaa gtgccagctt gaagcatcag cggctcttct atccttaatt tctcttccaa
     1081 ttggtgataa tagtcaaaat aacataaact catttatctc tcccgcctag ctatacttga
     1141 catgaataga aacagctgcc taatctaagg aagtacataa aaaaacagta gttagacagt
     1201 aacacatgtg gtctttgctg tttcaaacac aaaaaaggca ttgaacgaat acgcccaata
     1261 ccttgacata gcaaataaaa caacgtgcag accatggaaa atatctacat aggggtagaa
     1321 ttttcaccct agattaggta caataaatta ggtcgatgca ctgcattgac tatggcaaaa
     1381 tgatcggtca gggtgtatgt gtgtgactgg tcattccctg attcttagcg ccaactaagg
     1441 gtcagggagc ctttttttat tcagctgttt ctatctgaat ttattgtaag taatctctta
     1501 tccaagtgca agcaagaaaa tacaaaaaaa cattggtatg atttatatat atataaataa
     1561 cataaaaaaa ggaatggctt atcccgatac aacaagcatt cctttaaaaa agattctttt
     1621 atataagtga aaaaagcttt tttccaccaa ttaaataaat ctctcatctc aatagtttat
     1681 ccattatttt atcttttttt gcgtatacct actaaatatt accgtcttct tctcgttctt
     1741 ctactttgtt cgagagaatc tgtactagaa aaagtagttg tatgtctacc ttttctgcct
     1801 atccgagact ttcttttcat atatgggata taaatgatta gatacagtac agcaaaaaat
     1861 aaagcaaata cgcaagcttc ccaactacct aacgccaatt caaccatcaa ggtagcaacc
     1921 ccaacggatg ctgcatacca gataaaaatc agaaacgttt ctttttttct gaccatgcga
     1981 aacatgataa atagactaat aagcaatagt ggcaacacaa atatccaatt gcttccttcc
     2041 atcagccact tgagtatatc aagcagcgac tgtgcaacag cgactaattt tcccatagtg
     2101 aacacacacc tttttttata attaaaaaat tcgccttttt attctactat gaatcttctg
     2161 gcttggcttt catttctctt atgtaatcat tttcttttat acaacttaaa aaaaaattaa
     2221 accattagca taatattttc tgaatctatt cacgaaagtc tttttctatc tttcgttttc
     2281 cacgactcaa ctgatctaga gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa
     2341 gctgccgctt taataacaaa tagcctttca ttctcagcag gccacttatt tccagttaac
     2401 tttctgttct tagcttttta ctcaactgga taatatatgc acttaatgaa gtcccaattt
     2461 ccgaatgttg aagtccatat tcaatatttg tcttcatata tcctaactta tcgcctacgt
     2521 cgtatctttt tcctttaaac tctctcgcat agaccgcctc tgtctggtta agagtctgaa
     2581 gtgcatccgt tagctgtatc tcgtttcctg ctcctggttc ttgagtttct aatatctcaa
     2641 atattttagg agtcagtaga taacgtccga taatggctaa attacttggt gcttttccca
     2701 tttcaggttt ttctacaaac ccttttactc tgcaacgacc cttttcgcta tctgttttag
     2761 gatcaataat tccgtattta gatgtttcat tttctggtac acgcatgacg gcaacagcgg
     2821 aagcttgtgt ttcttcataa tccatcatca attgttgagt caatgggata tcatctttca
     2881 tcaaatcatc ccccagcatg acgacaaacg gttcatttcc tataaatgct tttgcttgaa
     2941 gcacggcatc ccccagtcct cttggatgag attgacggat gaagtgaaga ttgacttgag
     3001 tcgtttcttc aactaaagcg agtaattctg ttttttcctt ttcccgcaaa ttgttttcca
     3061 attcgatatt tgcatcaaag tgatcttcaa tgggtctttt ttcttttccc gttacgatca
     3121 agatgtcttc gattcccgaa tccaatgctt cttccacaat aaactggatc gttggtttat
     3181 ccacgattgg aagcatttct tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta
     3241 gcccagcggc aggaatgact gcttttttga ccttcacttg ct
//
LOCUS       DAZUMM010000140         3257 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000140 DAZUMM010000000
VERSION     DAZUMM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.150"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            209..445
                     /locus_tag="ACG8E6_002612"
     CDS             209..445
                     /locus_tag="ACG8E6_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFS5062967.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMMD
                     AIVKEESNLDLEIDMYVNQALSEYDSAFKELVDK"
     gene            442..855
                     /locus_tag="ACG8E6_002613"
     CDS             442..855
                     /locus_tag="ACG8E6_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS5062968.1"
                     /translation="MIYLNEKDIVKLNVIQIKKYSPQETIGVIDKNALSMAVNQPKQH
                     IFGESLYPDEFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMENQEVV
                     EFVVRIATYQGEFDDLKEATTDILKNNSTKFDTKK"
     gene            1398..2324
                     /locus_tag="ACG8E6_002614"
     CDS             1398..2324
                     /locus_tag="ACG8E6_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFS5062969.1"
                     /translation="MTGRNPKVFISYSWENKENKEWARELADKLLENGIDAYIDQYDL
                     SLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAELLSGKNERK
                     FIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKPALGDIPRH
                     IKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRVPSQLWKEL
                     FVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIEKSNTQEKK
                     ILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(2321..2917)
                     /locus_tag="ACG8E6_002615"
     CDS             complement(2321..2917)
                     /locus_tag="ACG8E6_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5062970.1"
                     /translation="MQQNPSIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQE
                     VLDFVRDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLD
                     KFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLV
                     YRSIVQDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
     gene            2978..>3257
                     /locus_tag="ACG8E6_002616"
     CDS             2978..>3257
                     /locus_tag="ACG8E6_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFS5062971.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAI"
BASE COUNT         1174 a          450 c          573 g         1060 t
ORIGIN      
        1 gaacttttct taaaaatttg aactttctta atttaaaaag attaaaatat ttaatagact
       61 tatatattaa taatctcaat gatagttgat aaattttaga tagttcgtcc tatatttgta
      121 aaagatatac ttaagcattg caatataaat attaagatat tgattaataa agatatacgg
      181 tatatacttg ttttagggag ttgattatat gaatattaaa aaaaggaaaa ttagagaggt
      241 aggcaactcg atagttgtca ctttgtcaaa agaaagttta ttacaaaaag gattgaaacc
      301 cggagataca atttttattg atgaagacaa aatgatggat gctattgtga aagaagaaag
      361 caatttagat cttgaaattg atatgtatgt gaatcaagct ttatccgaat atgactcagc
      421 atttaaggaa ctggttgata aatgatctat ttaaatgaaa aagatatagt aaaattaaat
      481 gttattcaaa taaagaagta ttcacctcag gaaacaattg gtgttattga taaaaatgct
      541 ttgagtatgg ccgtaaacca acctaagcag catatttttg gagaatccct atatcctgat
      601 gagtttagca aagcagcagt tctattaata aatctgatca aaaaacatcc atttcataat
      661 gcaaacaaac gtactgcatt tctagctgca tatgtttttc tcaagctaaa tggttactca
      721 ctaaaaatgg aaaatcagga agtcgtggaa tttgtagtta ggattgcaac gtatcagggt
      781 gagtttgatg acttaaaaga ggcaacaact gatatactaa aaaataattc aacaaagttt
      841 gatacaaaaa aatagaagca aactaggagt tcagtaaatt ctgaaatgtt agtttgcttt
      901 ctattttagt ttacataaga tacattatgc gaagttcatt attttaaaag cttaaataat
      961 aaactttttt ataataccaa tttatttatg taaaccaatg accttaatcg cttaagcaag
     1021 catgttatgt aatactctac tctctccaaa actaatgact atctattttc aaagaagcta
     1081 gaatgattat ctgatcaaat gataatttat atttaattcc tggtctactg ctcttctttt
     1141 aatcaattaa tgtctaacgt actgtacacg ataaagtagg aagacttatt ttcaacttcg
     1201 gattcatttt tttaattcgg tgaaaatcac taaattaaga gtttatccaa gttcatttta
     1261 gctgagaata cgcttgaaaa taattttgat gaattttcct gaattaaaat ttattaactt
     1321 gttgagtgat ataattgtaa tgtattacca aatagttagc acttagatta gtgatggaaa
     1381 ggaatagtga gaacaagatg actggtagaa atcccaaagt atttatatca tattcatggg
     1441 agaataagga aaataaagaa tgggctagag aactagcaga caaattactt gaaaacggta
     1501 ttgatgcata tatagatcag tatgatttaa gtttaggtga tagattacct cagtttatgg
     1561 agcagcaaat atccgattca gattatgttc taattatatg tacaccagag tataaaaaaa
     1621 aggcaaataa tcgtacaagt ggtgttgggt atgaaggtca tattatctca gcagaattgc
     1681 tttctggaaa aaatgaacgt aaatttatcc caataattaa agaaggacgt gcaagcgaag
     1741 tacttcctac ttttcttgct ggaaagcttg gaattgattt atcagatagc cagcattatg
     1801 atgaaaactt agatgattta attacaacta tatttggtgt taagagaaaa ccagcattag
     1861 gtgatatacc taggcatatt aaaggcagtc ctttactacc tatagaacat aatgaaaata
     1921 aagatattga aatcttagga attatcactg ataaagtaac tgtacctaaa atggatggct
     1981 caaggggttc tgcattatat aaaattccat ttagattaaa tagagtacca agtcagcttt
     2041 ggaaagagtt gtttgttaga aattggaatt caccaccacg atatacaaca atgcatagac
     2101 caggaattgc ttctgttgta gacgatgaaa ttttacttaa tggaacaact attgaggaag
     2161 tccgggaata tcatcgggaa actttaaaac tttgcattga aaaaagtaat actcaagaaa
     2221 aaaaaatatt gctagaggag gaacaaaaaa aagagagaga gaatgagcga gtcagatcac
     2281 ataaagaaag tgttcagaaa ttagcagatg aattagagtt ctagttgata gtcagttctt
     2341 tcttaattcg atacacagtt tgtctagtta cattatagtc tgttgctatt tttgaaatag
     2401 caacaccatt ctcctggtcc tgaacgatgc ttctataaac aaggcgacgt tgaggatctt
     2461 ttgtgttcgc actgtataac ttaggacgtc ctttatagac gccgtttgct ttagcaatct
     2521 ttatcccttg tgcctggcgt cgttttgatt cagtacgctc ttgttcagca atcatggcca
     2581 agatttgaat gattaggtct ttgataaatt tatccaggag aggatttccg attgcttcag
     2641 ccatgatcgg taaattagtg ataataagtc gcacattttt cttctttaga taatttactg
     2701 aatcgataat ctcgtcataa ttacgtccaa gtcgatcgat agcctctaca ataaaaatat
     2761 cttggtctct aacaaaatct agaacttcct gaaaaatcgg tcgatgagta atagtcgctc
     2821 cagattgttt ctcgatgaaa attttttcag caccaaattt tctcatttcc tcgatttgac
     2881 gttgttcatt ttgatcgatc gatgggttct gttgcaaagt tttccaaaaa atctatttta
     2941 gtgtaaaatt gagaaaaaag acagagagga cagagtaatg aatcatttta aaggcaaaca
     3001 attcaaaaaa gacgtcatta ttgtcgctgt tggttactac ctgcgttaca atctaagcta
     3061 tcgtgaagtt caggaattgt tatatgatcg tggaataaat gtttgtcata ctacgattta
     3121 tcgttgggta caagagtaca gcaaagtcct ctattatctt tggaagaaga aaaatagaca
     3181 atccttctat tcatggaaaa tggacgaaac ctatatcaaa attaagggac gttggcatta
     3241 tctttatcgt gcaattg
//
LOCUS       DAZUMM010000141         3137 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000141 DAZUMM010000000
VERSION     DAZUMM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.151"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..251)
                     /locus_tag="ACG8E6_002617"
     CDS             complement(21..251)
                     /locus_tag="ACG8E6_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062972.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="ACG8E6_002618"
     CDS             complement(687..1253)
                     /locus_tag="ACG8E6_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5062973.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="ACG8E6_002619"
     CDS             complement(1253..2209)
                     /locus_tag="ACG8E6_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFS5062974.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="ACG8E6_002620"
     CDS             complement(2209..2865)
                     /locus_tag="ACG8E6_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS5062975.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          980 a          608 c          555 g          994 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatg
//
LOCUS       DAZUMM010000142         3096 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000142 DAZUMM010000000
VERSION     DAZUMM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.152"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(23..1555)
                     /locus_tag="ACG8E6_002621"
     CDS             complement(23..1555)
                     /locus_tag="ACG8E6_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFS5062976.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(1664..2695)
                     /locus_tag="ACG8E6_002622"
     CDS             complement(1664..2695)
                     /locus_tag="ACG8E6_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5062977.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          940 a          560 c          549 g         1047 t
ORIGIN      
        1 ggatctttct ttccttaaat agttaggact tttgtgattt taattctttc tcgatttgaa
       61 gccgttgcac tttcattgta gctgttcgcg ggattgcatc aaaatcacgg atgataggga
      121 cgttcaagcg aggaagttca tggacttgct cccaccatcg attccaatcc atttctttat
      181 caggagctaa agcaataatc ggttgtggag aatgattttt atcccttaca atcactactt
      241 ctgccaaaaa atctaacgaa tctaataaaa agtcttcaat agctaatgta ctattgatcg
      301 tttcgatcaa atccacttgc cgatctttca aaaataggtg gccatgctta tccataaaac
      361 cgtaatctcc gctatcccac caatcgccat aaacattttc ctggaaccgt gcatcttcct
      421 tgtaataagt caaagcccgt cctttagaca gtaattgtat atgaccatct gtgttttcag
      481 gtaggacatt cccaactgaa tctgtaatcc gagcgcttgt catgtcttcc agtccaacgc
      541 ccatatctct tgcatcactt gttttcaaac tctctagtgt atgagcctta agaatcattg
      601 gtccgcattc actttggcca tatacttgaa gaaataccgc atcctgtgcc atcgacgttc
      661 gcaaaaatgt cttcattgtc tggttgttta tagcatcaaa agtggagtga tagtatcgga
      721 tacccgaaaa agcttctgga tgctccttag cagtaaaacg ccactgaaca aagttattcg
      781 gatgggtctc tagtgcaatt ggccggtgag cttccaacat atgaacaact tttgaacttt
      841 gtgcgtttgc taaaggcatc ataggaaatc ccatggacat caaagaagag ataccaatat
      901 tgaatctgga atgcactgga gagatgtgaa aagcgattaa tttcttttcc gaaatttttg
      961 tgaaaactgt tttttgccat ttcgtccgcc atcccataga atgagcagaa tgacaaataa
     1021 gtttagggat accagttgta ccagaagtat gggtcatata agaaatttca tttttgtcta
     1081 aaaatgattg agaaacagac aaagcaggtt gttccattaa ataaagaata ggaatctgtt
     1141 ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca tcaaataaaa
     1201 tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt agatgataag
     1261 aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg tatgtgtcaa
     1321 aagcggaact cttataaata ataactttgt ctccggattt cactccaaga tgagccaatt
     1381 gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca agtcctaatt
     1441 ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag ttagctgcag
     1501 cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc atcattcaca
     1561 ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat aatcctttat
     1621 ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg gaagaaaccc
     1681 gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag atatcttgaa
     1741 aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca gcatcatcta
     1801 aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat acgtttttat
     1861 atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc tgatcatgtg
     1921 aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta aaatagttct
     1981 ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt tcttttctat
     2041 tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct actacttcat
     2101 ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc ggtctattct
     2161 tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata aacaaatgct
     2221 caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt gtttccggta
     2281 aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac aaaaatcgta
     2341 aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt tcaaacaatt
     2401 gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag attttagcta
     2461 agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc acttcttcag
     2521 aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat tcatagcgac
     2581 tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg gaagggagca
     2641 agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg tccattacaa
     2701 attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat gaagatagac
     2761 agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa gatatgaagt
     2821 aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct tttaattgat
     2881 ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt ggttcgttct
     2941 aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt tcagaatcga
     3001 ttggctctcc atttattcaa taatggtttg tctaacgata ttgttgggat aattcttaaa
     3061 gaagtattag aaatagttgg tacttcatta ctttac
//
LOCUS       DAZUMM010000143         3088 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000143 DAZUMM010000000
VERSION     DAZUMM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.153"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(294..500)
                     /locus_tag="ACG8E6_002623"
     CDS             complement(294..500)
                     /locus_tag="ACG8E6_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062978.1"
                     /translation="MVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQ
                     ISSNLEDIKKAFKIKTIGTAPNFK"
     gene            638..1597
                     /locus_tag="ACG8E6_002624"
     CDS             638..1597
                     /locus_tag="ACG8E6_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5062979.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYCF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(1584..2189)
                     /locus_tag="ACG8E6_002625"
     CDS             complement(1584..2189)
                     /locus_tag="ACG8E6_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062980.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
     gene            complement(2429..>3088)
                     /locus_tag="ACG8E6_002626"
     CDS             complement(2429..>3088)
                     /locus_tag="ACG8E6_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS5062981.1"
                     /translation="YISLLCKEEVRELPKSNSAVGIDLGIIDFAILSTGQKFDNNKFT
                     SKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEKVMNQRTDFLNK
                     LSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYKADWYGRKIIKV
                     DKWFPSSQICSNCGHKDGKKSLEIRKWTCPICHTHHDRDINASINILTEGLRLHSMGL
                     A"
BASE COUNT         1007 a          513 c          478 g         1090 t
ORIGIN      
        1 taactaatta ttttgcagga ggacaattta tttacacaaa attattgacg ctcccttaac
       61 aataaatgaa gactttttta ttgttaattg tttatttttc tattattaga tatgaaaatc
      121 gcatttagat aaaatagaaa aatatgaata tattttaaat attaagatga gagtcattaa
      181 cttatgatag tagtcaattt gcttttcact aattacaaaa tatgtgatat aggaagttta
      241 gttattgttc tcataatgcc atcctgtttt tagattatct agtaattttt ttgttattta
      301 aaattaggag cagtgccaat tgttttaatt ttaaaggctt tttttatatc ttctaaatta
      361 gaagatattt gtatatcaat atggaatata ttaccttttt tgtatttttt tatgttatct
      421 ttagctaaaa catcaaatac atgtccttga aattgtatcg aggcagctgg atttagttta
      481 aattgaggaa actttaccac agctgattca aaacacttgt cacatttttt tattttttta
      541 ccttcgccga ttgtaaaatt aagctagaca actaaaaaag tcatttgtgc tacactcaaa
      601 atagttccgt cgaaagaatt ataaggagtg agtacaaatg acctataccc atcttacacc
      661 aactgaactc gtaatgatag aagcttattt caatcaatca cactctgttt caaaagtagc
      721 ccatctggta caacgttctc gacaaaccat ttataatgtc tattgttttc ttaaatctgg
      781 tggaagcgca ataagctact atgaacaata taagaaaaac aaacgtaact gcggaagacg
      841 tccgattgtg ttaccagatg ggcaacaaga atacattcaa aaaaaggtgg ctcaagggtg
      901 gactccagat gtccttattg gtcgagcaga gtttcctatt gcttgttcgg tacgtacact
      961 atatcggatg tttaaagata aaacctttga ccctcacgat ttaccaatga aagggaaaag
     1021 aaaaccaaac ggacataaag aaaaaagagg aaaacaagct tttcgccgtt cgattcagga
     1081 tagaacaact gattatatac agttcaatca tgagtttggt cacctagaag gtgacaccat
     1141 tgtcggtgga aaacataaaa gtgcggtgat cacgttagtt gagcgattat ctaaagtgat
     1201 catcaccttg aaacccgctg gtaggcaagc tatagatatt gagaatagac tgaatcaatg
     1261 gtttcaatct gtaccgaaac acttgtttaa atccatcaca tttgattgtg gaaaagagtt
     1321 ctcgaattgg aaaaagatta gtaataccaa tgatatttcg atttatttcg ctgatccagg
     1381 aacaccttct caacgaggac taaatgaaca ttcgaatggc ttattacgta aagatggctt
     1441 atataaagct atggatttta ataaggtatc cgaaaccttt atccaatcgg tggcatcaaa
     1501 acgaaatcat attcctagaa aatcattaca ctatcgcaca ccattggaag tatttttgag
     1561 ttacgtaggt aaagatgatt tgtctagctt aatttgacaa acgaaacttt gtaaaaatat
     1621 tcgattgaat attctatttg gcacgactca cttggcagta tatgtatgtc aatatttttc
     1681 atctgtttca taggtatttc aaaatctaaa ctattaaaaa catataattc cctgtaatta
     1741 tttacatcta actctactaa atttatcttt ggaacaatag atcctccacc cattccacca
     1801 taataaaaaa aatcttgttc tctatcaaaa tagcacatat taagctcagt ttgtttatca
     1861 tcctcaataa ttttataaat caaacctata taatcatgaa tgctggcaaa aatttttttt
     1921 aagtcaagtt ctaaatcttc ttcgaaaaca catttaattt ctcctactga taattgatac
     1981 tctatattac atgacttaat tagtttttta gatcccccct ccagataggc atatgtttca
     2041 ataattatgt tctttttatt gcttggcaaa tttccgttat ttattgcata aattaaaaat
     2101 gactgtctat ttagcccata atctaataca acttcttcat actcatattt ctttaaatat
     2161 ttaatatgaa aatatgctga attaatcaaa actcgagtgt tactgttgtt atttattgtt
     2221 gttctattgt gtataaaatt gtataggttg tcatattttc ttggtttagt gaactactca
     2281 ccacttagct aaacctaacg gtttcttacg tttgaagtgg gagcttcttg ggaagagagc
     2341 atacttgata gcgtattact ttactaacag aggtgcctct tatttaccaa gctatccccg
     2401 tagttcctac ggttcttgtt tatttgatct aagccaatcc catagagtgt agccttagac
     2461 cttcggtcag gatattgata ctagcgttga tatctcggtc atgatgagta tgacaaatag
     2521 ggcaagtcca ttttcgaatt tcgagagatt tcttgccgtc tttatgtcca caattcgaac
     2581 agatttggct agatggaaac cacttatcta ctttgatgat tttccgaccg taccagtcag
     2641 ccttgtattg taatttagac acaaaactag accaagaaac atccgaaata ctttttgcta
     2701 gtttatgatt acgcaacatg ccttttgtgt ttaagtcttc aatacagatg atatcgtgat
     2761 ttttgataat ttctgtactc aacttattca gaaaatcagt acgttgattc attacttttt
     2821 catgcaatcg tgctacttta cgtttttgtg tttgataatt tctagcttca aacagattga
     2881 tacctttttg tttagctaat aaggcacgtc ttgacaactt acgttgttca cgtttgagtt
     2941 tcttttccat tttggacgta aatttattat tatcaaattt ttgaccggta gaaagaattg
     3001 caaagtctat aatacctagg tcaataccaa cagcagaatt agatttaggt agttcgcgaa
     3061 cttcttcttt gcataacaaa gaaatata
//
LOCUS       DAZUMM010000144         3044 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000144 DAZUMM010000000
VERSION     DAZUMM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.154"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(104..1051)
                     /locus_tag="ACG8E6_002627"
     CDS             complement(104..1051)
                     /locus_tag="ACG8E6_002627"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5062982.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDVDSVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1296..1760
                     /locus_tag="ACG8E6_002628"
     CDS             1296..1760
                     /locus_tag="ACG8E6_002628"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFS5062983.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQQILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1822..2865)
                     /locus_tag="ACG8E6_002629"
     CDS             complement(1822..2865)
                     /locus_tag="ACG8E6_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFS5062984.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     RATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGIKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         1004 a          603 c          583 g          854 t
ORIGIN      
        1 aagctacttc tggaacacca tttattcgga aaaaaggact gtgacaagac ttttgtcaca
       61 gtcctttttc atttaaaaaa atgtttactt catttgacgt atattattgg tgaggcttca
      121 attcatttgg cttcactttt tctgtttctc ttatgatata aacaggacgc tgtttcgttt
      181 ctaagaagat tttcccgata tatttcccga tgattccaag acataacaat tggattccac
      241 caatgaacag gaagatcgag accatggaag gccatcctga tgtaggatca ccaaacaaca
      301 atgcccgcaa aataatgaac aaaattgcaa taccagaacc gacacaagac aaagcgccga
      361 taaatgaagc aatcgttaac ggtgcatccg agaagttcac gataccatca atcgagtagt
      421 tgaataattt ccagaaggac caagatgttt cgccagccac acgttcgcgg ttttcataag
      481 aaatatattc tgttttaaat ccgacccagc taaataatcc ttttgaaaat cggttgtatt
      541 ctgttaattc taggacggca tccaccattt gacgagtcat caagcggaaa tcacgagcgc
      601 catcgaccat ttctgtatcg ctgattttat tgatcaactg gtagaatttt ttcgcaaaga
      661 aactgcgtat cgcaggctcg ccttcacgag tcgaacgccg ggtgccgaca ctatcgacat
      721 cagaagtagt gatccggttg atcatctcag gaagcagttc gggcggatct tgcagatcaa
      781 catccatcac ggtcactaaa tctcctgtag aagcttgcaa tccagcgtac agagccgctt
      841 ctttcccaaa atttctggaa aatgagacaa agcggacata aggataagct gaggctagct
      901 tcttgattgc tgctaacgta ccgtcttttg atccgtcgtc aataaacaaa tattcgaact
      961 catgggcacc tttgaatttt tcaacctcat caaaaaaaag aggaacagtt ttttcttcat
     1021 taaaacatgg gacaacaatt gatactttca tcgtttctat cctttcaatc gtatgtttat
     1081 atatggttta cattcaaaat ttaataaacc ttaaataaca ccttttaaat ttgattttat
     1141 ggaaaagaac agctattctt tggtaaacta acgtatttag tttaccatgt ttcctcggtt
     1201 tatcaaactt ttaagaacaa tttttattag ttggcaagta gttttggata gagaaaaagg
     1261 aggaagttcc agtctactga ccaggagcaa acaaaatgaa aatacacaca cctatcggct
     1321 ttttatggtt agatgcagat gaatccggat tgactgctgt atcttttcag ccaatttcag
     1381 aaagcgaatc agcgaatcag caaattttaa cgcgcacaca agaacagttt gaagaatatt
     1441 ttgcgggaaa aagaaaagaa tttgatgttc ctttatctat aaagagaggt acgccttttc
     1501 agcaaaatgt ctggaaagct ttgtcgacca ttccttatgg agaaatccga acgtataaag
     1561 aagtagctgt tgctgttgat agtccgaaag ctgtgcgagc gattgggcag gcaaatcgaa
     1621 ccaatccatt gccaatcgtt attccttgtc atcgagtgat cgggcaaaat ggaaaactga
     1681 caggatatat gggaaacgca atagatgggg ttgaaatcaa acgccaattg ttgttgttag
     1741 aaggatatct gcaaaattaa aaacagttgt gattcggaca ttgttccatg cgagaatcac
     1801 aactgttttt tcatgttcat tctaaacttt gatcagccaa ataaatagta aagccatcac
     1861 tgtttggaga agcccgacag ctaatccata agataagaag cgtgtacctt ctttcatgaa
     1921 ttttgcaaag tctaaacgca gaccaattgc cgctaaagca gtgatctcaa accagctgct
     1981 gataaaatgt gccgattggt caataactac aggtaaagaa aacaagctgt tgaccaaaca
     2041 aaagatcaca aaccctaata cataccaagg aagtggtaat tttcgttttt tctgcgtatc
     2101 agttggcgtt tcaaacagag ctggttgttt cttgtgcatg aatttttcaa acagatagac
     2161 aacaacaacg agcatcataa tacgtagaat cttaaatagc atggcgaatt cgacaacaga
     2221 attgtcaagt aagcttgccc cagcgactac ttgaccaaca gattggagtg tgccacccaa
     2281 aagtgcactt ttttcaatga gatttccacc aaacaactgt aatccaagga aagggagagt
     2341 caacatcatc acagtaccca gtagattgac tagtgtgatg atctgtcctt tttcttcctc
     2401 tttcgcatgg atggaagggg cgatcgaagc aatagcagaa gaaccacaaa cagcgtttcc
     2461 tccagccatc atcaaagaag aagtatcgct gaattgcagt tttttaccaa tcaagtaagc
     2521 accgatgata gtgactgaca tttgcagtaa cacaaaaaca attcctttta taccaatttg
     2581 agcaatcgtt tgagctgtga cagttgcacc tagtaaaaaa acagagaatt ctaatagttt
     2641 gctttcagca aattttgtgc cgattgctag aacaggctgt ttaaaaaaag tatttcctaa
     2701 taaaataccc aataaaatag caatagtcgc tctacctaac tgtggcagat aaatagcaag
     2761 aagcctgctg aaggcagcga tggcaaaaga aagcagtaaa ccgggtaaga tttcgcggac
     2821 cgctgaatcc tttacagatt ttattggatg tgacaatgat ttcatttgat cggttccttt
     2881 cgtcaataaa tactaaaagc atactcgaac atagtagcac taaaccagta ttttgacaaa
     2941 agaacaagag gtcgtgacaa aagtcttgtc acgacctctt gtttcgaata aacggtgttc
     3001 aaaagccagt ttctcggtat taagccaaat ttatgcaaaa atgt
//
LOCUS       DAZUMM010000145         3007 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000145 DAZUMM010000000
VERSION     DAZUMM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.155"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            124..1137
                     /locus_tag="ACG8E6_002630"
     CDS             124..1137
                     /locus_tag="ACG8E6_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5062985.1"
                     /translation="MTKKNRVTIRDVAQAAGVSISTVSKALNSLDGVSPETRVEIQNI
                     ASRLNYVPNLMGKQLKSGKTKMIGVYTNRISGPYFNSLIDEIAFESKLHGYGLNVIVS
                     DERDVIMSSLLGNFVDGAIVVEDVLNQKDIETINSNGVATVFINRIVADKKVGSVAFD
                     SFEKGYLAGNYLLNLGHKKIGYVNGVDDIYDNDQRFQGFKAALSKVGLTYNEDFTIAG
                     NFVEEDAYANTLAFIDNQPAELPSAFLAANDMSAVGLIKALTEKNYSVPEDFSVVGFD
                     DVEVLKYFKPSLTTIHNSTKEQGKKAIEHLLEMMGGKSVGRVIEVKGTLMIRETAIEY
                     TGE"
     gene            1144..1494
                     /locus_tag="ACG8E6_002631"
     CDS             1144..1494
                     /locus_tag="ACG8E6_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5062986.1"
                     /translation="MYVGIDVGGTSIKYGLVDSKGNIVAKDTLVASFEKEELLQNLAD
                     IVEKYVSSSPETIEGVGISMPGIVQEDGFLLTAGAIRSCYGINLKEEMEKRTGILTKE
                     VIDSNGANLDWENK"
     gene            1597..2817
                     /locus_tag="ACG8E6_002632"
     CDS             1597..2817
                     /locus_tag="ACG8E6_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003030771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HFS5062987.1"
                     /translation="MNKKSAILLLSCGVFLSLATERSFATEENKTNHVSAETALVATS
                     KTTVSKDNPTVFDFPFNGEDNRMHRADGWSNGNMFNTTWRASQVNFEDGKMQLSINKD
                     EENTKIPYISGEYRSNQNFHYGLYEVNMKPIKNDGVVSSFFTYTGPSENNPWDEIDIE
                     FLGKDTTGVQFNYFTNGLGNKEYYHNLGFDASEAFHTYAFEWLPDSITWFVDGEKIYT
                     ATENIPQTPGKIMMNVWPGTGVDSWLNPFDGTVPLKAEYDWIKFTQYKIVEEDVTPKP
                     EEPTTEPTTEPTTEPTTEPTTEPTTEPTTEPTESSTTGSSTTESSTTNSSGNTTPPAT
                     TETTNTTSSSTSQSSKAAGKTTTPKNNSTTSKKDLPATGENTHASYWTAFGGMLILAG
                     LGGALFFKNKNRKA"
BASE COUNT         1046 a          496 c          678 g          787 t
ORIGIN      
        1 acaaaggcgt taaattagct tttatttttt ataaaaaaag cttgtatcca aaaaaataca
       61 gtgttatact tcaatcgaag taaagaaaaa cgttttcttt aaaaagtaca tgaagtgaat
      121 aaaatgacaa aaaaaaatcg ggtaacgatt agggatgtgg cacaagcagc tggcgtttca
      181 atctcgacgg tttccaaagc gttaaacagt ttggatggcg tttcacctga aacccgggtc
      241 gaaattcaaa atattgccag ccgtttaaac tatgtgccca acttaatggg gaaacagtta
      301 aagtctggta aaacgaagat gatcggggtt tatacgaatc ggatcagcgg tccttatttt
      361 aactcactaa ttgatgagat tgcttttgag tctaagttac atggatatgg tttaaatgta
      421 attgtctctg atgagcggga tgtaattatg agtagtctgt taggtaactt tgttgatggg
      481 gcgattgtgg tggaggatgt cttaaatcag aaagacattg aaaccattaa ttccaacggc
      541 gtggcgacgg tttttattaa ccgcatcgta gcggataaaa aagtcggcag tgtggccttt
      601 gattcgtttg aaaaaggtta tctggcgggg aattatttat taaacttagg tcacaaaaaa
      661 attggttatg tcaacggggt cgatgatatt tacgataacg accaacgttt tcaaggattt
      721 aaagcagcct tatcaaaagt cggtttgact tataatgagg actttacgat tgccggtaac
      781 tttgtagaag aagatgccta tgccaatacg ttagccttta ttgacaatca accagcagag
      841 ttaccgtcgg cctttttagc ggcgaatgat atgagtgcgg tggggttgat taaagcctta
      901 actgaaaaaa attattctgt accggaagat tttagtgttg ttggttttga tgatgtggaa
      961 gttttgaaat atttcaaacc aagcttaaca acgattcata attcaacgaa ggaacaaggt
     1021 aaaaaagcga ttgaacattt attagagatg atgggtggga aatcagtcgg tcgtgtgatt
     1081 gaagttaagg gcacgctgat gattcgagaa acagcaatag aatacacagg agagtgattg
     1141 agtatgtacg ttgggattga cgttgggggc acaagtatta aatacggttt agttgattca
     1201 aaaggaaaca tcgtggccaa agatacgtta gtcgcgagtt tcgaaaaaga ggagctctta
     1261 caaaacttag cggacatcgt ggagaaatat gtaagcagtt cgcctgaaac aatcgaaggt
     1321 gtgggaatca gcatgccagg gattgtccaa gaagatgggt ttttattaac cgctggggcg
     1381 attcgttctt gttacggtat caatctaaaa gaagagatgg aaaaacgtac ggggatttta
     1441 acaaaagaag tgattgattc aaacggggct aacttagact gggaaaataa ataagtcaca
     1501 aagaaacgtc aaacattaac agaaaaataa atgtttggca tttcctttac gaaaaaatga
     1561 aaacgattgc ttaaaaacaa taactggagg gaatttatga ataaaaagag tgcaatttta
     1621 ctattaagct gtggggtatt tttaagtttg gctactgagc ggagcttcgc aacggaggag
     1681 aacaaaacaa atcatgtgag tgctgaaacg gcactagtcg ccaccagtaa aacgacggtt
     1741 tctaaagaca atccaacggt ctttgacttt cctttcaatg gagaagataa ccggatgcat
     1801 cgggccgatg gttggagcaa tgggaatatg tttaatacaa cgtggcgggc ctctcaagta
     1861 aacttcgaag atggtaaaat gcaactttcg attaataaag acgaggaaaa tacaaaaatt
     1921 ccgtatatta gtggggaata ccgttccaat caaaacttcc attacggttt atacgaagtt
     1981 aatatgaaac caattaaaaa tgatggggta gtgtcatctt tctttacgta taccggccca
     2041 agtgaaaata atccctggga tgaaattgat attgagtttt taggtaaaga tacgactggc
     2101 gtacaattta attattttac gaatggcttg ggcaataaag aatactatca taatttaggt
     2161 ttcgacgcct cagaagcatt ccatacgtat gcctttgaat ggttaccaga ttcgatcaca
     2221 tggtttgtcg atggtgaaaa aatctatacc gcgactgaaa atattccgca aacaccaggc
     2281 aaaattatga tgaatgtttg gccgggtact ggagttgact catggttaaa tccctttgat
     2341 ggaacggtac cattaaaagc cgaatacgac tggattaaat ttacgcaata taaaatcgtt
     2401 gaggaagatg tcacgcctaa accagaagaa ccaacgacag aaccaacgac agaaccaaca
     2461 acagaaccaa cgacagaacc aacaacagaa ccaacaacag aaccaacaac agaaccaacg
     2521 gaatcttcaa caacgggaag ttcgacaacg gaatcttcaa caactaacag tagcggaaat
     2581 acgacaccac cagcaacaac tgaaacaact aacacaacct caagctcaac tagtcagtcg
     2641 tccaaagccg caggcaaaac aacgacgcca aaaaataata gtacaactag taaaaaagat
     2701 ttacctgcta caggagagaa tacacatgct agctattgga ctgcttttgg cggaatgctt
     2761 attttagctg gtctaggcgg cgccttattc tttaaaaata aaaaccgtaa agcgtaatcg
     2821 gaggggattt atgaaagcga aaggtgttat gctagtatta ggctgtagct tattgcttgg
     2881 tttagtaatt aatcgcagtt tggcccatga gaaaaaaagt aaaaatagtg gcgaggagac
     2941 ggagagtacg atggtagaaa ataaaaataa cgctaaagct cagacaactg tctttgactt
     3001 tccacta
//
LOCUS       DAZUMM010000146         2954 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000146 DAZUMM010000000
VERSION     DAZUMM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.156"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..1251)
                     /locus_tag="ACG8E6_002633"
     CDS             complement(61..1251)
                     /locus_tag="ACG8E6_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5062988.1"
                     /translation="MNYFTTSLMKNLFSNDNNLPAFEQAVTELFRSELEKSLNEILAY
                     ELTDFLDYEPYARTNNENSRNGSYQRKFDTKYGTITLNIPRDRMGEFFTSLLPKYRRR
                     DLFTETTILDLFEEGMSHSEICSMIQKLCGARYSKQTISNITDKALECVDSFKNRILN
                     KEYAVVYLDGTSMPLRRDTVSREMVHIALGITVEGTKEILGYMIAPNESAEIWEELLK
                     DLKSRGVERISLFCTDGLSGMENVIQDIFPGSKIQRCLVHIQRNICAKVRVSDRKEVA
                     NEFKEVYRSKSKEEALTSLKKFIDRWKHKYPSMVKSLSNNDYLFTFYDYPESVRHTIY
                     STNLIEGNNKQLKRSFKKKEQFPTEKSEEKYLVIQFNRYNEKNMNHVHRGFGQTTRED
                     WFKS"
     gene            complement(1650..2951)
                     /locus_tag="ACG8E6_002634"
     CDS             complement(1650..2951)
                     /locus_tag="ACG8E6_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5062989.1"
                     /translation="MENSAFIMKLNDILSPIAAKIGNQRHLKAISSGMMFGLPFIVIG
                     SFFLIFANPPINIDKYNPDTANIFMKFMASWKDFAVANYDLITAPYNLTMGIIGFISV
                     FGIAFSLANEYKINPSMNGMVASVIYLMVCTPVEEGAINLNFLGTNGLFVAIIIGLLV
                     VEINRLFEVKNIKLKLPDTVPPMVATFINMLVPLLTNIFIFYGINLIFLAATNKIFPE
                     AVMEVLTPATNIAGSLGGFLLIVTLGNILWLFGINGSSIIFPIVFTLGMAQTGLNAEQ
                     LANGETMTHLMNLQMFRISVLGGAGGTLGLVLLMMRSKVPEYRTIGKLSLVPGICSIN
                     EPVIFGLPIVFNPILAIPFLITPIISLLLTYFAQQLGWIGIGFIVDPSFTPFFAQAYL
                     SSMDWRNILFCLLLIVISIFIYYPFFKVMESNKTKLVDEKL"
BASE COUNT          910 a          524 c          535 g          985 t
ORIGIN      
        1 atcgtttaaa tttgggagcg tcaataattt tgtgtaaaag tatatccttg gtaaagcaga
       61 ttagctcttg aaccaatctt ctctggttgt ttgtccaaaa cctctatgta cgtgattcat
      121 gtttttctcg ttgtaacgat tgaactgaat cacaaggtat ttctcttcag atttttccgt
      181 tggaaattgt tctttctttt tgaaacttcg ttttaattgt ttattgtttc cttcaatcag
      241 atttgttgaa taaatcgtat gcctaacact ttctggataa tcatagaatg taaacagata
      301 gtcattgttg ctgagacttt tcaccattga tggatatttg tgtttccacc tatcgatgaa
      361 tttctttaga gaagttaaag cttcttcttt tgatttggac cgatatacct ctttaaattc
      421 attggccact tcttttctgt cactgactct tacttttgca catatatttc tttgaatatg
      481 gaccaggcat ctttgaattt tagagcccgg gaaaatgtcc tgtattacat tttccatccc
      541 agacaatccg tctgtacaaa ataaagaaat acgttcaacc cctcttgatt taaggtcttt
      601 cagaagttct tcccatatct cagcggactc gtttggtgca atcatatagc caagaatttc
      661 ttttgtacct tctactgtga ttcctagtgc tatatggacc atttccctgg atacagtatc
      721 acggcgtaaa ggcatggatg ttccatcaag atatacaacg gcatactctt tgttcaatat
      781 tctgtttttg aaggaatcta cacattccag agctttgtcc gtaatattag atatggtctg
      841 tttagaatag cgagctccac ataatttttg gatcatggaa caaatttctg aatggctcat
      901 tccttcttca aataaatcca aaatcgttgt ttcggtaaat agatctcgtc ttctgtattt
      961 aggcagaaga gaagtgaaaa attctcccat tctatctctt ggaatattga gtgtaatagt
     1021 tccatatttt gtatcaaact ttctttgata ggaaccattt ctagaatttt cgttattggt
     1081 tctagcgtat ggttcataat caagaaaatc cgtcaactca tatgcgagta tttcattgag
     1141 agatttctcc aactctgaac gaaacagttc ggttacagcc tgttcaaaag ctggaagatt
     1201 gttgtcatta gaaaataggt ttttcattaa agatgtagta aaatagttca tgggtatatt
     1261 cctttcgtaa attgtttttg gtcgaactaa ttttactaag gatatacctt tttttctaca
     1321 tttctagaaa aacgaattta cacaagatat tttatgctat ccatttattt ttgaaactcc
     1381 atttgtttta ctacgtgtat tacctttatt tttctttcta tttttaggtg tctattatct
     1441 cttacgttcg cttataaatg atgactcaaa atttaaagca aaataaaaat aaaggaagta
     1501 gccttttgaa caagggcgct tcctttattt gttgtgggac accagagtat acttttttat
     1561 aaaaaatagg ttgatgggaa accctaagat ttcccatcaa cactaaatag tatagaacca
     1621 tattttcgtt acaaggttaa gaatgacgac tatagttttt catctactaa ttttgtttta
     1681 ttgctctcca tgactttaaa aaatggatag tagatgaaga tgctgatgac gatcaacagt
     1741 aggcaaaaga gaatattgcg ccagtccata cttgaaagat aagcttgtgc aaaaaaaggt
     1801 gtgaaggaag gatcgacgat aaatccaatc ccaatccatc ctagttgctg agcaaagtag
     1861 gtcagcaata atgaaatgat gggcgtaatc aaaaatggaa ttgccaaaat agggttaaaa
     1921 acgataggta atccgaaaat cactggttcg ttgatggaac agattccagg aaccaatgac
     1981 aattttccaa ttgtgcgata ttcgggtact ttgcttcgca tcatcaataa aaccaaaccc
     2041 aaagtaccac cggctccacc aagtacagaa atacggaaca tttgtaagtt cattaagtgt
     2101 gtcatggttt caccatttgc cagttgttca gcattcaaac cagtttgagc cattcccaat
     2161 gtaaagacga tagggaaaat aattgacgag ccattgatac caaatagcca taggatgtta
     2221 cccaaagtca cgatcaataa gaaaccacct agactgccag cgatgttcgt tgctggagtc
     2281 aggacttcca tcaccgcttc agggaagatt ttgtttgttg ctgccaagaa aatcagattg
     2341 atgccataaa agatgaatat atttgtcagc agtggaacga gcatattgat aaaggttgct
     2401 accatgggtg gaactgtatc tgggagtttc aacttaatat tttttacttc aaatagtcgg
     2461 ttgatttcca ctactagcaa gccgatgata attgcgacga acaatccgtt tgtcccaagg
     2521 aaattaagat tgatggcacc ttcttccact ggtgtacaaa ccattagata aatgacagat
     2581 gcaaccatgc cattcatgga aggattaatt ttgtattcat tggcaaggga aaatgcaatg
     2641 ccaaaaacac taatgaatcc gatgattccc attgtcaaat tataaggggc tgtgatgaga
     2701 tcataatttg ctacggcaaa atctttccag ctggccataa atttcatgaa aatattagct
     2761 gtatctggat tgtatttatc aatattgatg ggtggatttg caaaaatcag aaaaaaagaa
     2821 ccgataacaa taaagggtag gccaaacatc ataccgctgg atatagcttt taggtggcgc
     2881 tgattaccta tcttggctgc gatggggcta agtatgtcat tcagtttcat gataaacgca
     2941 gaattttcca tata
//
LOCUS       DAZUMM010000147         2947 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000147 DAZUMM010000000
VERSION     DAZUMM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.157"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(449..865)
                     /locus_tag="ACG8E6_002635"
     CDS             complement(449..865)
                     /locus_tag="ACG8E6_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062990.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKI
                     EKQLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
     gene            complement(867..1148)
                     /locus_tag="ACG8E6_002636"
                     /pseudo
     CDS             complement(867..1148)
                     /locus_tag="ACG8E6_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1948..2922
                     /locus_tag="ACG8E6_002637"
     CDS             1948..2922
                     /locus_tag="ACG8E6_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5062991.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     QEGKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQ
                     LRRGKATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYK
                     YRELLKQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
BASE COUNT          914 a          437 c          603 g          993 t
ORIGIN      
        1 aaaaaaactt gctgatgaat tgggtgtatc gaaaaatacg gtgcgcaatc aactggtcaa
       61 ttcgggattt gaccttgctc agtataaaga aaaaaatgca gtggtcaatt gaccgaaatt
      121 tatgtctttg ggttttgggg ttttaaaacg aaaaaaaggg ggcgattttc gccccctttg
      181 ctctatgtga aaaatttgtt atacttattt tatccttaaa acataccaat ctttttctta
      241 tttagctgct cgtacgagca gcttttttta cgattatgaa ttcaaatcat gtatttataa
      301 tttttttgtt tttatctttt tttctgttat ttcttctgaa attcgttttt ttcattcaat
      361 tggttactat cttattgtaa atgttgctat taagatggtg atcctaccca aaaaataaac
      421 caggaattta aatctcctgg tttatttttt atctttttag aaaatttgtt gggtcgaaat
      481 ttttagaagt aaatgtttga ttggtttctt tttgacgatt ttttggttga tttcgtttgg
      541 agggattaag ttgtttttca attttttctt tcgtttcttg atacacttta gaatttgtgc
      601 caataagaat acctgtatct tttagatata aatcagcaaa ttctgcaatg atttcggaca
      661 tgatggaaat ttcttttccc atagcattta gtttggcttg gttgctggta gtcgtagtat
      721 tggtatcagg ttcataactc atacataaaa aacgaatggc ttccgagtaa ttgttgatat
      781 ctggatttct ttcgataatg gaatctaaaa cgtccacgtg actgggataa agggtaacgt
      841 gtttttgaac agaagttttt ctcatgttat cttcctccga taagtgtttc aatgagttgg
      901 ttgtttgtac gaacatattc attcagcgtt tcagtatttt tttcttgaat agcgataaat
      961 ttttcgagta ataaatcgaa ttgatctttc ttgtaatgca taagtgcgtg gtcaaaatcg
     1021 ttttcgatta ggacttttaa gtagcgattt cgactccatt ttttgccagt tttatttgtg
     1081 agttattggc aacgtttgtc tatttttgca ataacctttg aatcgatctt ccgaactaaa
     1141 atgtccatag aaaaactcct ttcgcatttg tgtataattt atgtatatca tacgaatata
     1201 gtatttgtat agctgttttt tgctaaaata ctatacataa gctagccacg atggcgaaaa
     1261 agtgatatca aatttttttc tgagaaacgt gataggcaca gcctatctac ccactttttt
     1321 tagaaagtgg gtacacgttt ccttatgctc tttataaaat ggtgatgtgc gacttttttc
     1381 tgttcgtaaa aataaaaaaa gtttacgaaa atgaaactaa actgaacgat tatgacgggc
     1441 atgtaatcaa gatgaaatga ttttataagc gcgtattttg cgttctaaga cgttttagcg
     1501 ttcatttcgt ttagttatcg gcacaatcgt taaaacacgt aagagaagcg agaatggagc
     1561 ctgtaaggcg actggtagcg aaacggcgtg ttagattgtg caagccgaga actgaatgaa
     1621 atgaagagcg gaagcgtgaa ataatttcgg caggaggagg ctcaaggggt tcggggaatg
     1681 aaattccctg aggtcttttt atttttttaa gaggagtgct ataatcaaag aaaacgagaa
     1741 aaaagagcaa aataaaagcc gaacggtgtt ggaagcaccc ttcggctaat tacggaaact
     1801 actcacctag ttccgtaact caaattcatt atacgaatat ttttaggttt cttcaagcta
     1861 gagatttttg gatatggatt tttagagtta cgtgaactct ggaaattctt tccaaaaatc
     1921 tcttttttct ttgaggagga acaaaatttg agtaaacgtg gcacgatcat aaatgagtgg
     1981 gaaaaactta accgcatatc taacaggcgg ttaaatgcac aaactatgtg caccccctta
     2041 aaaatgcaat ggtcaattga ccgaattacc attgttggta cattaaggga aaatatttat
     2101 tatcacactc cccatgatgt gttgatttta gattttgaac agcttatgcg tctaaacgaa
     2161 ggtaacggat atttaaaagc ggttggaaat aatggctggc aattactaga ccaacatgag
     2221 gaaaatatag cttatattga gattcttaag tttcaagagg gaaaagggcg gattgacttt
     2281 aaccccaata aaattagcca atttctggcg ggctcgatga agaactttat ccatgattta
     2341 tttttagaac cacatttctc acgtgcagat attgcttgtg acattattga cgtgccagat
     2401 gatttcatta cccaatatcg ggtggttgat cctgtttctt tcaaaccaat ttacggtcgg
     2461 tctgggaaac tggaaacagc atattgggga agtcgtgcta gtgaacgaca gattcggatg
     2521 tataacaaaa aattggaaca agaaagaaaa cggaaagttg ttccgaaaga aatcgatact
     2581 tggtggcgac tagaattgca attgcgaagg gggaaagcaa ctgattggca tgcaatggtt
     2641 catgaaagtt tagatagctt tgcaagtcct cactatttgc cagaagatgt gaaagtaact
     2701 gacagaatga tgattactgc tcttataacg gaacatagtt actgggggca aattcataga
     2761 aattcaaaat ataaatatcg tgaattgtta aagcaagaaa gtcaaaatga tgagttgacg
     2821 aatcatttgc gagaaacatt ttcggagtcg gcagatgatt tgaaacgaga attagataca
     2881 tggcttcaag gacttgatgt gacggaagta ggtgctgaat gagtgattcg atagaaaaaa
     2941 tgaccct
//
LOCUS       DAZUMM010000148         2891 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000148 DAZUMM010000000
VERSION     DAZUMM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.158"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..227
                     /locus_tag="ACG8E6_002638"
     CDS             <1..227
                     /locus_tag="ACG8E6_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5062992.1"
                     /translation="KTTEFDILHALMSTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHI
                     KNLRHKIENDAKSPMYILTVHGTGYRFGGH"
     gene            227..1620
                     /locus_tag="ACG8E6_002639"
                     /pseudo
     CDS             227..1620
                     /locus_tag="ACG8E6_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294791.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1635..1841
                     /locus_tag="ACG8E6_002640"
     CDS             1635..1841
                     /locus_tag="ACG8E6_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062993.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            1862..2458
                     /locus_tag="ACG8E6_002641"
     CDS             1862..2458
                     /locus_tag="ACG8E6_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HFS5062994.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
BASE COUNT         1049 a          396 c          572 g          874 t
ORIGIN      
        1 ttaaaacaac tgaatttgat attctacacg ctctaatgtc cacacctaat aaagtctttt
       61 ctcgtagtga tttgatagag aaagttaagg gaatagaatt tgatggacta gataggagca
      121 tcgattctca tataaaaaac ttaagacata aaattgagaa tgatgctaaa tcacctatgt
      181 atattttgac agtccatggt acaggataca gatttggtgg ccactaatga gaaaaagaac
      241 gataaaaact caattagctg tttccttttt agcaatcgca acattaataa taggatcgat
      301 tagtttagtc gcattaagtt tgatgaacaa tcattttagt aaatacgtgg aagagcggca
      361 agaagatttg ttgaatcagt atgtatatac aattgattta ttgtggttaa atagtgggga
      421 aacatggaat agtgaagaac ttgcagcact atctgagaaa gtattagaga ataatattta
      481 tttttctata gaagacgaac aaggcaacat ggtttgggaa cttactggaa aagatttgaa
      541 gtcagcacag gagaaactga aaaaaatgca ttgaaagttt ctgagaaaaa cagtgtgaaa
      601 ctagacgaaa caatagaagt gaaaaagaag ttaattaatg atggaaacga gtttggaaaa
      661 gtaacgtttt attatttcgg accttttgct tatacagagc acgatgcctt gtttatctcc
      721 agtatgaaac aaagtttaat gtatgttgca attgcagctc ttcttgtatc attcatactt
      781 gcttcgtgga tctctgctag attaggattg ccactaaagc atgtaagcga ttttacacat
      841 aaattaactc gtggggaata tgctgataaa atcccacaag aaacttcaat tattgaaata
      901 aattccttaa ttgattccct aaatgattta agtaatcagt tggagaaaca gcatgggctt
      961 cgaaaaagat taactaccga tatttcacat gaattaagaa ctccattagc tacgcttaaa
     1021 ggcaatgtgg aaggaatgat tgacggagtc tggaaaataa ctcccgagcg cctacaatct
     1081 tgttatgacg aaatagatcg tctaactcgt ttaattggaa atatagaaat aatcaacaaa
     1141 attgaagcaa aatatgatca tttaaataaa actgagttta acatatataa gttgattgaa
     1201 tccgttattg aaaatttcgc cagcaaaatt gagtcaaaaa accttcatgt tgaaatccaa
     1261 ggggataaca tcaatatatc tgcagataaa gataaaatga accaagttgt tatcaatctt
     1321 ttgaataatg ctattaaatt tacgcaaaag gagggaacca ttaagttttc tataagtaaa
     1381 aataaagacc atgttctttt aattgttgaa gacaacggca tcggtataga aaaagatcag
     1441 caacttcata tttttgatag attttatatg gctgacccct ccagaagtag agctttaggg
     1501 gggcaaggta tcggattagc tattgtaaag agcgttattg aagcgcataa aggtagtata
     1561 actgtaaaaa gtaaattagg tctaggaact aaatttgtta taaagttacc atttcaataa
     1621 aggaggagag ttatttgttt caaaaacaga taaaaattgc taaactagta tgtgaatgtt
     1681 gggcaaaaaa aatatttttt caactgattt cgatagatag tgtcgaaaaa gttcatttag
     1741 atatcttatc aaattctgta gtaatctttt ctaagaagga cttatcaata gctacaatta
     1801 aaaaagaagt ttttggatta caaatttatg agatgaccta aatgtgatta attaaaggag
     1861 gatgaagaca gtgaaaaaga tgacaaaatt agtatttgca agtcttggaa ttattgtatt
     1921 tttaggagcg tgtagcaatc aaagtgaatc gaataattct aatagcacaa acgaggaatc
     1981 gacttctaca gcgtctagtg aaatgaatag tatggaggga atgaatcacg aaggtatggt
     2041 tccttcttca atgaaagatg ctgcgaaccc taaattcccg gtgggaagta atgttatact
     2101 gctaggcgat catatggaag ggatgaaagg tgcaaatgct caagttgtag gggcctttga
     2161 tactacaatg tatgaagtaa gctatgagcc aaaaactggg ggaccaatgg ttaacaatca
     2221 tcgatgggta gtacaagaag agttaaaaga tactgagact gtagcaaatg agggcgatac
     2281 tgttatcttg aatgccgatc acatggatgg gatgatggga gctgaagcaa aagtagataa
     2341 atctattaca ggaactgttt acgttgttaa ttacactcca accgatggtc aagaagaagt
     2401 taagaatcat atgtgggtaa ctgaagatga gatggaatat gacgaaaata atgagtaatt
     2461 ttatatacta aaccaacaag ctggtacatg gtcttcgtat tctcaacatg aggaatatga
     2521 gctctttgtt aataaggact gatgagttgt gcaaaatcaa atctgggtca gaacgaacct
     2581 cattctggct cagatttttg gctcttcgac atttagtgct ggtagagaga caactgaaaa
     2641 tttagtcata accagttagg cagagtgctt aactggtttt tgttcgcatt tattattctt
     2701 ttgatattgg ctgcttgccc ttcaatttta tttggtttct taatcaactc ttcatataac
     2761 cccaaatcat atattaatga ggtgcctcct attcctataa atctatctct tctttaatag
     2821 tatgagaaaa gtctactctt gtagatttaa attgtcaaca aaaaatttac agatgtaaat
     2881 gataagttga a
//
LOCUS       DAZUMM010000149         2844 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000149 DAZUMM010000000
VERSION     DAZUMM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.159"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(106..468)
                     /locus_tag="ACG8E6_002642"
     CDS             complement(106..468)
                     /locus_tag="ACG8E6_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5062995.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(542..922)
                     /locus_tag="ACG8E6_002643"
     CDS             complement(542..922)
                     /locus_tag="ACG8E6_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFS5062996.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1004..2179
                     /locus_tag="ACG8E6_002644"
     CDS             1004..2179
                     /locus_tag="ACG8E6_002644"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFS5062997.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2256..2840)
                     /locus_tag="ACG8E6_002645"
     CDS             complement(2256..2840)
                     /locus_tag="ACG8E6_002645"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5062998.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          881 a          552 c          481 g          930 t
ORIGIN      
        1 taaaaaacta tctttagcag aacatttcat tgtatttttt ggaccaaaca ataaaaaaag
       61 aagtttgaaa aaaattcaaa cttctttgtt cgcttaatct gtattttaga agaaaggcac
      121 tccctctact tcgtaagttc caagaacaga gcgcatttga cctggttctt cccaaatata
      181 tccttcgcct aattcttctc tttgattctt ttgttcagtt tcaaagacaa acaacggaac
      241 attttcctct ttaatatgtg tatttgtcaa ttccatcaaa ctaatcttgc taacatcgat
      301 atgaactatt tcttttaaga aattaagtat attcgctaaa ccagtctttc cttcagaaag
      361 ttccgctaac gcaaattcca acttgtcatc aattgaatgg accaaaaatt ttttagtacc
      421 atcctccaaa tgcaacataa ttgtaccagc aactcttttg cttgccaaaa tttccacctc
      481 gatcaacttg ttctatggct tacataaaaa ttctagcaca ttttcagaaa aaatacactt
      541 tttatttctt attttctttc cgttctagtt cagataatgc ttcttctatc caaataggta
      601 actgctccgc aatagtggta aatccgatct tacgattttt attcgtgaaa tacctttttc
      661 tgcgatatgg aacagctgga tttgtttttt ctaacgttct taaagataaa ctgatttttt
      721 tggaatattc atcaatgtcg atgatctgaa ctgttactgg ctgtccgact tttaacaatg
      781 aatggatatt tttcgtataa ccggattgta cttctgaaac atgaataagt ccttgcgtct
      841 cgttatcaag agaaacgaat gcgccataag gctgtatccc tgtcactttt ccttcaatta
      901 tttgtccaat tttataagtc atctacttcc ctctcttcgc atttatcatt atactgtaaa
      961 tagctcaaaa aaagaagcga cgaaaatgac tggaggtctt aagatgatcg aatttgataa
     1021 acagatttca agaaaagata cagaaagtgt aaaatgggac gcaattgctg agacatatca
     1081 aatggatgac ttacttcctt tgtgggtagc agatatggac ttcttgtctc ccggtggggt
     1141 agctaaagcg tttagtgaat acatcaaaca tggaatcttt ggttatgcca ctctgtctga
     1201 taaactatat caggctatca ttcattggga acgccaacac catgctgtgg agttgacgaa
     1261 agaaaatatc gtttttacaa gtggtgtgtt aaccagttta gctgcagcag tacaaacttt
     1321 tacaaaacca ggcgattcta ttttgattca tgacccagtt tatcctccat tttcctctat
     1381 tatagaaaca aatcaacgtc acatcgttcg aagctctttg ctagaaaaaa ataatcattt
     1441 catcatggac ttgtctgaca tggagaaaaa aatcgaagaa aacaatgtaa aagcaatgat
     1501 tttatgcaat cctcacaatc ctggagggcg tgtttggagc aaagaagaat tgaaacagtt
     1561 aagcgaattg tgtttaaaac atcaagtgat tctgtttagt gacgagatcc atcaagattt
     1621 aactttattc gatcatacat ttacttccat gctaacgatt gatcctaatt tagatgatct
     1681 actagttgct tttacttccg caaccaagac tttcaatcta gctgcaatca aaaattcaat
     1741 ggtatttatc aaaaatccag agatgaaagc tgattttgaa aaacatttag tgatgaacca
     1801 acagcacgaa atcaacactt ttggactgat tggaacccaa gcggcttatg agacgggtgg
     1861 agaatggtta agccaactga ttccttatct agaaaaaaat gttcaaacag ttaatcagtt
     1921 ttttgaacag tatcttccga aagtgagaat aatgaaccct gaaggaactt acttaatgtg
     1981 gttagatttt accgcctata tttcagacga ccaagaattg gagaacactc ttgttcggaa
     2041 aggaaaagtc gtattaaatc ctggcatcac gtttggtcct tccggtcatg ggcatatgcg
     2101 cttgaatatt gcttgtccag aagaaacttt actggaagga cttaatcgaa tcaagtatgc
     2161 tttagagaca atcgattaaa tcatttattc aaaataaaag gacgagacct tactaggttt
     2221 cgtccttttt cataattact ccgtaccata ccaaactatt ctgaaatttc aattgtttca
     2281 atcactacat catgtacagg tcggtcttgt ggaccacgtt gtacatttgc gatttcatct
     2341 actgtgtcca tcccttctag tacatgtcca aagactgtat gtcggaaatc tagccaagga
     2401 gttccgccac ctttatacgc ttcaatgatt tctgatggga atccagcacc ttctagttgt
     2461 ccaagcatat tagctggcac atttgtgtta tttacaataa agaattggct tccgtttgtg
     2521 tttggacctg aatttgccat agataaagct ccgcgaaggt tgaacagttc acgactgaat
     2581 tcatcttcaa aactttcgcc ataaatactt tcgccaccca ttcctgttcc agttggatcg
     2641 ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa tcacgccatc ataataacct
     2701 tttttggaaa gttcaatgaa attttgaacc gtcttcggtg ctagttcagg gaataattga
     2761 acagtaatgt caccacggtt tgttttgata acagcttttg gtcctttttc actttttaaa
     2821 tctagttgtg ggaatgccat attg
//
LOCUS       DAZUMM010000150         2772 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000150 DAZUMM010000000
VERSION     DAZUMM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.160"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            213..1100
                     /locus_tag="ACG8E6_002646"
     CDS             213..1100
                     /locus_tag="ACG8E6_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS5062999.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(1694..2389)
                     /locus_tag="ACG8E6_002647"
     CDS             complement(1694..2389)
                     /locus_tag="ACG8E6_002647"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFS5063000.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(2373..2771)
                     /locus_tag="ACG8E6_002648"
     CDS             complement(2373..2771)
                     /locus_tag="ACG8E6_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS5063001.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT          931 a          503 c          414 g          924 t
ORIGIN      
        1 atccaatcaa ccgaataatc ctgatcatct ttcatccctc ctttatatta agtttaacaa
       61 cccttttctt tcttgtctcg tgaaaactat gtaaatgttc tccatcatag agagaataga
      121 tttttacgaa ctaagaatat ctacagcaac cttacgaaaa tgaaagactg tctccctaat
      181 tcttctgtta gactgttttt aataaggaaa ggatgggaaa ttttttgaaa aaacaactac
      241 taattatgtg cctttcagca tgtgcaattt tactattctc aggttgtagc aagtccaatg
      301 aaacgattgc aacgtttaaa ggtggaacac ttacagttca ggatttttat gataacccaa
      361 aagttaaatc aaataatcaa acactattaa aaaaaatgat tgtcttcaaa gcatttgatg
      421 atgtatacgg aaaagagatt tcttcaaagg aagtaacaaa agaatataat gaacaaatca
      481 aaaaactcgg tccaaactac aaggaacaat taaaagcagt tggccaaaca gaagagacat
      541 ataagaaact ctttaaacag atgctggctt tccaatacgg tttgaaagcc aatgtaaaat
      601 taacagataa agatctagac actgcttgga aagaattcta tccagaagtt agtacacaga
      661 tcattctatt ttcgactaaa gaagaagcag ataaagcaaa aaaagaagca aatgaaggag
      721 aaaatttctc caagcttgtt caagcttatg gaaaaaataa aacattaaag gaaactgatg
      781 gaaaaatgaa ttttgattca accaatccag agattcctac tgaagtaaaa aaagcagcat
      841 ttaaactaaa aaacggggaa gtttctgata tcattcctgt aacagatcca acgacttatc
      901 agcaaagcta ttatctagta aaaatggtaa aaaaacagga taaaggttct aataaagaca
      961 aatacaaatc tgagctagaa aaaattgcta ccgaagcaaa aactcttgat acggaattca
     1021 tggataaaac aatccgtaaa gtgatgaaaa aagacaatgt aactattaaa gatccttatg
     1081 taaaaaatat attcaaataa acaaatttga cccaattggg gatgtggcaa aatttgagaa
     1141 gtgatttttg gaaattcctt cttatttttg aggcaaacca cttttgccac aacttcatcg
     1201 gggccttttt ttatttgtac caaaacaaat cactggattt gttacaatca tgaggatata
     1261 atttaaaaaa gttttttatt tctatcacat gaatcaatgc gccattaagt agattctttc
     1321 atgcttccct attttttcac ttgccgtatc gcttcaaaaa caactatcat aaactcaaac
     1381 tcgactgaaa agttatttta aaaaagacta aaaatatccc ttctggactt gttcttctta
     1441 ttctgttata ctagagaaac acgaaagata tttttatgcg acacttttca tttgttttta
     1501 tgaaatttat tcaaaactgg cgtaacttca gagttacaag gatgtattag aggttaggga
     1561 atacatcgtt taaatgagaa ataagtaata gttagtaata gtcacccaaa ctttaaatta
     1621 atgaatattg aatcgtcaga tagcctcctc gagttttcga gaaggctatc gtttatttta
     1681 tggagaaatt gattcagaag taatcagcga ccttttttag ttcaccaatc gtttctgctt
     1741 ggaaagtttg cacccaatct tcttggaagc ccctcactgc tttatcaatc gagggcatct
     1801 ctaatacttg atctagtagc tctttaccta acgttatcgc atgagctcca gatcgaatgg
     1861 ctgtagtgac ttgttcacaa tttttgaaac tagctgctaa tatttttgtt ggtctttgcg
     1921 tacgttcgat ctcagtccgc aacgaatcaa tcactttcat agcatcaatg ccttgatttt
     1981 gcatcctgtt ataataaggt gcaatataat ctgctccatt attaatagct aaataccctt
     2041 gtaattctgt gtaaatagct gtaccggtaa tcaaataacc ttcttttttc aactttttga
     2101 tcgcttgaag gcctgcttga ttcaccggca ccttgataaa cgtatctttt cccagttctt
     2161 ctatgataac tcgagcatcc tctatcattt cttcttccgt tcttcctatt gtctgaatat
     2221 ggataggaat ctgtccgatt atttttttga tttctttcat atgcgagaag aagtttgacg
     2281 ctccttcttt tttcagaatt gtaggatttg ttgttacgcc tgttaaattc aaaaactgtt
     2341 gacaagaata aatacttttt gtatttgctg tatcaagcat gatttccata tagctttccc
     2401 ccattctgat cctcttgatc gattttaatg attgtcccct ttcttaaatg cggcatttcc
     2461 ttatcttcta catacattgt tcctggaagt tccggctcat gagatccatc aaattttatc
     2521 gtgatatgtc ctaattcatt caaattagtt tggacaacat ctcctactgc tgtgattaca
     2581 tacatttgtt tatcaaaaca gagcttcata tcacaagtta tttcttttct tacctgattc
     2641 aattcaatct tataacaata atctgttaat gtttccggtg cttcatctcc aaataaaatc
     2701 attatttttt ctttcctgaa tacttctgct tctataccaa catctgttac aaccgtttca
     2761 aagatcgtca tc
//
LOCUS       DAZUMM010000151         2639 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000151 DAZUMM010000000
VERSION     DAZUMM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.161"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1816
                     /locus_tag="ACG8E6_002649"
     CDS             <1..1816
                     /locus_tag="ACG8E6_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063002.1"
                     /translation="DKFYTIFTEEYSQNKLELINNKLTSTDKSVYDLYCYPLNEFVQK
                     QPISNSILLQYHKLQKEDFLDSVGVFYTDLQANEIREIESQLAVTISVFENEAIPYSM
                     ILELYLLNFVILFVVLQIIYCIYTSDSLKKKGIKKSMGFSAMHILKEQINNIVKYFIV
                     ICSVLFILLNLYYALTNRFNISYLTISIFFFAIILVINILCVLITSFLIKFVSLEAMI
                     KNKTLNSGTNLIVQTIKIVFSVIITITTISLLNQGNEFKQSQQDILDYKYLDGYFTAN
                     GFNSLEYEYALENPEQFEEYSNSMLELYNNHHALLCDYAGSSEAQGEFSKSRPYYIQQ
                     TIIANRNYISEFSNIQVNGKSLNEDIYDTPTILVSRKYKSDETFIKEHITQEYFRLMN
                     YNQFYGIPEKERHINEFNIVYVDDNSTIKVNTENGFSDMSEPIIIIDTGNFAGLYYLD
                     SLNTRNLFFQMDSREEFSSLLAEYGLKQLVTAGTLLTPYLMQLENVTFVLKTLTMFAI
                     VFIISLLFILYISNYVDIFVNRKKYATKEIMGFSHFKTLKNRYIFWGIELIASCILTA
                     INYNFACLFAIILIDYIFCELLYRIYILNALYEIEKGA"
     gene            1819..2469
                     /locus_tag="ACG8E6_002650"
     CDS             1819..2469
                     /locus_tag="ACG8E6_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5063003.1"
                     /translation="MSLITLKNIKKVYGKNQILFENLSLEIEKGEFVGLVGKSGAGKT
                     TLLNIIGLITDISEGIITIDGQQKLSVNSRNAMLLRRYTIGYLFQNYGLIEDESVLWN
                     LKLALEYKKMKKEDKLKVINEYLKQFNLSDMLNKKIYQLSGGEQQRIAIIKLILQGSQ
                     IILADEPTSGLDKENEQVVMKLLKELNEKGITIIMVTHNMDLRNYFSRVINVETFR"
BASE COUNT          968 a          312 c          421 g          938 t
ORIGIN      
        1 tgataaattt tatacgatat ttacagaaga atattcccaa aataaattag agcttatcaa
       61 taataaactt acttctacgg ataaatctgt ctatgattta tattgttatc cgctaaatga
      121 atttgtgcaa aaacagccta tatcaaattc tattctgttg cagtatcata aattgcaaaa
      181 ggaagatttt ttggatagtg tcggggtatt ctatacggat ttgcaagcca atgaaatacg
      241 tgaaatagaa tctcaactgg ctgtaacaat tagtgttttt gaaaatgaag ctatcccgta
      301 tagtatgata ttagagttgt atcttcttaa ttttgtaatt ttgtttgttg tacttcaaat
      361 aatatattgt atttatacat ctgatagttt gaaaaaaaaa ggtattaaaa aaagtatggg
      421 cttttctgct atgcatattt taaaggagca aatcaacaat atagtaaaat attttattgt
      481 gatatgttcg gtgttattta tcctgctgaa tttgtattac gcattaacaa atcgttttaa
      541 tatttcttat ttaacaataa gtatattttt ctttgcgatt attttggtta taaacatact
      601 ttgcgtttta attaccagct ttttaattaa gtttgtttct ttggaggcaa tgattaaaaa
      661 taagacttta aatagcggta caaatcttat agttcagact ataaaaatag ttttttcagt
      721 aataatcaca attaccacta tttcattatt gaatcaagga aatgaattta aacaaagcca
      781 acaggatatt ttagattata aatatctgga cggatatttt acggctaatg gttttaattc
      841 tttagaatat gaatatgcat tagaaaatcc agaacaattt gaagaatatt ctaattcgat
      901 gttagaactg tataataatc atcatgcctt gttatgtgat tatgctggta gcagtgaagc
      961 acaaggggaa tttagtaaat ctcgtccata ttatatacag caaacaataa ttgcaaatag
     1021 aaactatatt agtgagtttt caaatatcca agttaatgga aaatcattaa atgaggatat
     1081 atatgatacg ccaacaatat tagtttcccg taaatataaa agtgatgaaa cttttattaa
     1141 agagcatata actcaagagt attttcgact gatgaactac aatcaatttt atgggatacc
     1201 tgagaaggaa aggcatatca acgaatttaa tattgtgtat gtcgatgata attctactat
     1261 aaaagttaat acagaaaatg gcttttcaga tatgtcagaa cctattatca ttattgatac
     1321 aggcaatttt gcaggtcttt actatttgga ttctttaaat acaagaaatt tatttttcca
     1381 aatggattca agagaagaat tttcttcttt attagcagag tatggtttaa aacaattagt
     1441 tacggcagga acattactta caccatactt aatgcaactg gaaaatgtga cctttgtcct
     1501 taaaacactc acaatgtttg cgattgtctt tataatatca ttactgttta tcttgtatat
     1561 ttcaaattat gtagatattt ttgtgaatcg aaaaaaatat gcaactaaag agattatggg
     1621 attctctcat tttaaaacat taaaaaatcg ttatatattt tggggaatag agttgattgc
     1681 atcatgtata ttgactgcta taaattacaa ttttgcttgt ctttttgcaa ttatacttat
     1741 tgactatata ttctgtgaat tactttatag aatttatatc ttaaacgctt tgtatgaaat
     1801 agaaaagggg gcataagtat gtcattgatt acattaaaga acataaaaaa agtatatggt
     1861 aaaaatcaaa ttttattcga gaatttgtcg ttagaaatag aaaagggtga atttgttggt
     1921 cttgtaggta aaagtggtgc aggtaagaca actcttttaa atataattgg attgattaca
     1981 gacatatcag agggaataat tactattgac ggtcaacaaa aattatcagt aaacagtaga
     2041 aatgcaatgc ttttaagacg atatacgata ggctatctat ttcaaaacta tggacttata
     2101 gaagatgaaa gcgtattatg gaatttaaaa ttagctcttg aatataaaaa aatgaaaaag
     2161 gaagataaac tgaaagtaat caacgaatat ttaaaacaat ttaatttgag cgatatgctc
     2221 aataaaaaaa tatatcagtt aagcggtgga gagcaacagc ggattgcaat tatcaaatta
     2281 attttacaag gaagtcaaat tatactagcc gatgaaccaa cttctggact tgataaagaa
     2341 aatgaacagg tagtgatgaa acttctgaaa gaattgaatg aaaagggcat tactataatt
     2401 atggtaactc ataacatgga tttgcgtaac tatttttcca gagttataaa tgtagaaact
     2461 tttagatagt ttattaaagg gagatatact tttttgtgca atctcccttg ttctttttct
     2521 gtcatatcaa gactcgttca ataatggtaa attcgtggta aaatagctac ttttttagtt
     2581 gatgaatagc ccttaaatac tgtatttatg cggataatcg gattttttat accaagttt
//
LOCUS       DAZUMM010000152         2607 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000152 DAZUMM010000000
VERSION     DAZUMM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.162"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG8E6_002651"
     CDS             complement(<1..182)
                     /locus_tag="ACG8E6_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5063004.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG8E6_002652"
     CDS             517..951
                     /locus_tag="ACG8E6_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5063005.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG8E6_002653"
     CDS             974..1294
                     /locus_tag="ACG8E6_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063006.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG8E6_002654"
     CDS             1435..1626
                     /locus_tag="ACG8E6_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063007.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG8E6_002655"
     CDS             complement(1697..1954)
                     /locus_tag="ACG8E6_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFS5063008.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACG8E6_002656"
     CDS             2112..2375
                     /locus_tag="ACG8E6_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063009.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG8E6_002657"
     CDS             2399..2587
                     /locus_tag="ACG8E6_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063010.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
BASE COUNT          999 a          371 c          536 g          701 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaaga
//
LOCUS       DAZUMM010000153         2408 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000153 DAZUMM010000000
VERSION     DAZUMM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.163"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..2272
                     /locus_tag="ACG8E6_002658"
     CDS             119..2272
                     /locus_tag="ACG8E6_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFS5063011.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFAPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKALELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYTPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDITTVNSNIVAYGPTNNPEI
                     AISVVLPNLLDEQDHMNLTIAKEIMDAYYDMFMAK"
BASE COUNT          821 a          424 c          531 g          632 t
ORIGIN      
        1 aagttgattt tatcttgaaa ttaggtaaag aatttcttgc tccgcccatt ttttcttttt
       61 tattaagaag tttttgggta caatgaagca tgaatgtaaa taggcggaag gataggaaat
      121 gatgaaagat tttatgaaaa aaatattaaa tacttcttgg ttagaaaaaa taagaaaagg
      181 tgcacaagaa cctaagaaca aaacgtttcg ccggtcccat gttccttttc gcttaaattt
      241 tctttttttc attatattta cgttatttgt tgcgttgatt gctagattgg gttatttgca
      301 gatcgtgaac ggcgaagaga tggaggcgcg agtgaagtct acttctacga ttacggtcca
      361 agaaagtact cctcgcggaa tgatctacga ctccacagga aaagctttag ttacgaataa
      421 agcgaatcag gccatcacat atacgagagg aacacaagtt acggctaaag atttgcttga
      481 aatcgcaacg aagctgaact cgctgatcga tgttccggta gatgaaaatc tgacagatag
      541 agataaaaaa gatttttggc tggctgatcc caaacatttg aaagaagcca caaaaagact
      601 ttcggcaaaa gaaaagcaat tagggaacag cgaacaatat agtgcgatgg tcaataaagt
      661 cacagctgat gagattaatt taaacgagga gcaattaaaa attgctacca tctttaaacg
      721 gatgaatagt gcatatgaat taaacactgt ctatatcaaa aactctggtg ttactgatca
      781 agagttagct gttgtggcag aacgcgcttc tgaacttcca ggagtttcga ctggcacaga
      841 ctggacacga gaatataatg cagcaagttc actgaaaagt attcttggca gtgtgacgac
      901 tgaaaagcaa ggacttccag cagatgaagc agagaaatac ctagcaaaag gttattctcg
      961 gaatgaccgt gtaggacaaa gttatttgga aaaacaatat gaagatgtcc tgcaaggaac
     1021 caaaacccaa tatgaagttt cccttgataa tgaaggaaat gtttcgaatc aaaaagaaat
     1081 cttctctggg gaaaaaggtt ctaatttaat gttatctatg aatgcagaat tccaatcaaa
     1141 agtagaagaa attttgaaaa ggaattatca aacattgatc aacaacggca aagcacagta
     1201 ttcgcctggt gcttatgcag tggctatgaa tcctcagaca ggagaagtgc tagcaatgac
     1261 tggtttcagt catgaacaag gttccaaaga aatcaccgag aatgctttag gcacgattac
     1321 tagcgccttt gctccagggt ctgtagtcaa ggctggtact ttgacagcag gctgggcaag
     1381 taacgcgatc agcggaaatc aagttttgat tgatgagccg attcgtctac aaggtgcttc
     1441 agaaaaaagc tctgtcttta atcgaagcgg acaagttgct cttgatgcag tcaaagcatt
     1501 ggaattgtct tctaatacgt atatgatcaa agtggcgtta aagatgttag gactagacta
     1561 tacacctgga atgggactac ctagtttaga tgaagaagct aaagcttatc agcaattgag
     1621 agactctttc aaagagtttg gtcttggtac aacgactggg atcgatttgc cgaatgaatc
     1681 tcctgggatc tcacgttctg ttgattatat gaagaagttc aatgcggata atggcaaaga
     1741 atggtatact ccaggtaact ttaccgattt ggcatttgga cagtttgaca catatacacc
     1801 aatccaatta gctcaatatg cttcaactgt cgctaatggc gggaaacgag tacagccgca
     1861 cctagtcaaa gcgatctatg gcaatgacga aaatggaaac ttgggtgaag tgaagaaaga
     1921 aattggcaca acagtagaaa atacggtcaa tatttctgca gaaaatatgg cgatcctccg
     1981 tgaaggattc caccaagttg tccatggtac cgatccttac acaacagcca aaccattagc
     2041 cagtgcgaaa atggaccttt ccgcaaaaac agggactgca gaaacggttg cggaaggtca
     2101 tccagatatt acgacagtca atagtaatat cgtggcatac ggaccaacaa acaatccgga
     2161 aattgccatc agtgttgtct tgccgaactt attagacgaa caagaccaca tgaacttgac
     2221 gattgctaaa gaaataatgg acgcgtatta tgatatgttc atggcaaaat aaaaaagagg
     2281 tcgtgaaaaa gccgcttagt ttcgagtatg aagaaaaatt ttgtgaaaat agcttctcac
     2341 atttttgctt aaatttgact tcataccgag aaactggctt ttgaacaccg tttactcggc
     2401 aaaaaagg
//
LOCUS       DAZUMM010000154         2372 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000154 DAZUMM010000000
VERSION     DAZUMM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.164"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..249)
                     /locus_tag="ACG8E6_002659"
     CDS             complement(64..249)
                     /locus_tag="ACG8E6_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063012.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            464..835
                     /locus_tag="ACG8E6_002660"
     CDS             464..835
                     /locus_tag="ACG8E6_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5063013.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(985..1057)
                     /locus_tag="ACG8E6_002661"
     tRNA            complement(985..1057)
                     /locus_tag="ACG8E6_002661"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1022..1024),aa:Gln,seq:ctg)
     gene            complement(1103..1531)
                     /locus_tag="ACG8E6_002662"
     CDS             complement(1103..1531)
                     /locus_tag="ACG8E6_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063014.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(1591..2349)
                     /locus_tag="ACG8E6_002663"
     CDS             complement(1591..2349)
                     /locus_tag="ACG8E6_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS5063015.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT          738 a          410 c          441 g          783 t
ORIGIN      
        1 aaccaaagga ggacgatgca gcagcattgt cctcctttga ttttacaaaa gtgaaagttt
       61 tacttatttg aatagatttt taaatttgtt tttgacactg tcgatattta catcttttaa
      121 aagattttgg gctttctctt tataaggtcc taattgttct ttatgttctt cgataaatcg
      181 ttttgcgcca tcgaaattgc cgttcttaat catttccttg actttttgaa ataattcttc
      241 ttgattcatt ttgaacacat cctttgctat caacatgatt ttttacatgg acaaaacgag
      301 aaaaactgat ttatccacac gtcaataata tcaaataacg atcattttta ctaaaaaaac
      361 actcgattct cgcttctaaa gtaggagaaa aatctccaac ttgggacgga tggaatttct
      421 ttaactgacc catatactga atatataaca aggaggtaga aagatgaaag tgattaaata
      481 tttgattgga ctggcggcga ttagtggtat tttgctattt ggactgaaat attatactga
      541 aggaagttat ggagaaatcc ctggtatact tgatcagcta aatccattag ttgaaaaagg
      601 agaggtctat gtaaagacac aaaaaccaga ggaagtaaat gaatatggta cagcaaggta
      661 tgtgcagaaa gctgctgatg ccaatgggaa agaacgaacg gttgaattta atggtctttc
      721 tgtgttaaaa gaaaatcatt atttgaaaat tagcaataaa ggagcacaca tagaaacgta
      781 tgaggaagtt cctagagaaa aagttccgga gaaagcatta gcaattattg ggtaaatcag
      841 tttttgtatt aagaatacgc ccgattttat tattcataat ttaattatgt aaactaaata
      901 caagtagtaa aaataataca ataatccccc tgcaaaaaga ttgttctttt acagggggtt
      961 cattttttca gcaagcacat caaattggga tggcagggtt cgaacctaca attgcttgat
     1021 tcagagtcaa gtgccttacc agtttggcta catcccattt attcaacaaa ataatcatag
     1081 cactttccta aaaaaacgca agttattttt cttgatcgga tagttgttgc tttactcgta
     1141 aaaggcggcg tactagtttc ttttctaatc cagcaggaag ggcaatcagt tctgctactg
     1201 acaagacggt gtccaataac tgatattctt tgacaggttc attcataata tgatagatgt
     1261 ttgctaaacg ttcaatatat tcaaagcgag tatactctgt tcgagcaatt agaaatttca
     1321 ataaatcggt ggctttctcg tactcttgat ctgcgtaata aacaagagat tgacgataca
     1381 atttgatttc cagttcattg agggtagcag gtctgcgacg ctcgttccac tcattaatag
     1441 agactttacc ttgaacattt cccttgcagt cacggacaaa aataatcgga atttcatttg
     1501 gatccatttt tttatgtgtg aatttttcca ttatctacgc tcccttcaaa ggtttggtat
     1561 cttgagtata agggattttt gtttgaatta ctagcttttc tccaaagtta gcatctgtcc
     1621 acccattgca gtgaaaatcg ccgaaaaatc gttgatcggt actgctcgat tttttacacc
     1681 atagggatcg ttgacataaa tattttcttt gtcatatcca gtgactacac aagcatgaac
     1741 aagaggagtg actcttactt ctccattatt tgtggtccaa gtcataaaat cagtttcatc
     1801 tgggacttca aaattgatag tcacagaagt ccagacagga tttcctttag atagtgcttt
     1861 tttgatatca tcgagttgag catcggtttt tggaaaagtg tgataatccg tttggacgat
     1921 ttgctgagca agggaggcaa ctgggggaac atagacgacc atagcccagt ctccgccact
     1981 gatatcacca acaaatcctt cattaggatc gccatgagtc ccatctgcat tgaaaacagg
     2041 gacgtaatcc aattgctcag ctaactggtt tttagttgtt tcataaccat aaaaagataa
     2101 aagcatagaa agcgaagtga cttcacagcc attttctaaa gaaggagaat caaactggct
     2161 ctctaatgga acatctagta aaacttctgc tttctctttt gtctttttcg ttttttgtgt
     2221 tgaacttttt tgtgcagtgt tcattgtatg ttctggagaa tggatatgta ggtaataact
     2281 tgtaccgctt aataaaagag cagctgtgcc ccctagaaac aatagttttt tgaacgtttt
     2341 tttcttcatt tgaaatctcc cttttcagct gt
//
LOCUS       DAZUMM010000155         2368 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000155 DAZUMM010000000
VERSION     DAZUMM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.165"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            202..1815
                     /locus_tag="ACG8E6_002664"
     CDS             202..1815
                     /locus_tag="ACG8E6_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5063016.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          747 a          419 c          453 g          749 t
ORIGIN      
        1 ttcagctttt aaaaaatata cagggttatc cccttcaaag tattatctat ccttaaaata
       61 gaaaaaaatt tttaggtttt atccacaaat tatttctcta tattttctgt ctattcaaaa
      121 taaacaataa ttttccggca caattatttg ctgtttttta ccataacata gtacgatcaa
      181 cgtagaaaag gagatgtttt tatgaaagtt gttgtcattg gcggtgtagc tggcggtcct
      241 tcatttgcca ctcgtttccg tcgattgaat gaagcgcacg aaatcattat ctatgaacgc
      301 ggagaaaata tttcttatgc aagttgtgct ttgccttatt atttaggagg tgtgatcact
      361 gaccgtgact cgctgatcga acgtacacca gaaatattga aaacaaaaaa caacatcgat
      421 gtctttacta aacacgaagt aacagcaatc gatccttcta ctaagcgatt aacagttaaa
      481 gacctatcca caaatgaaga aataaaaaca gattacgata agttgatcat ctcttctggt
      541 gctagaccag attatccgga tattcccgga gtttttgaag cagaaaacgg ctttgtcctc
      601 cgtagtgtga cggatgcgga tcgaatcaaa tcgttccttg aagaaaaaaa tccacaacat
      661 gtcgtcattc ttggtgcagg tgttatgggt ctggaattag ctgagaattt caagcatcgc
      721 ggcttagacg tgactttaat cgatcaattg ccacaagtcg ctttccctta tgatccagaa
      781 attgctaatt tagtttatga caaattgctg aaagaaggat tagccgttca tttagaaaca
      841 agagttactg agatccgtga taaaggtcga gaaatagtat tatcagatgg ctccgtcctt
      901 tctgctgata tgctaatttt tgctgttggt gtttctccga agaatgaagt ggtgaaagca
      961 accggcatac aattatcaga tacaggtcag atcattgttg atgaccagtt acaaaccaat
     1021 ctgccggaca tctatgcgat tggcgatatt atcgaaacga ctagtgtagt gactggtcag
     1081 ccgatccaaa gtatgctttc cagtgcggcc aatcgtcaag gacatatgct ggcagatatt
     1141 ttaaatggta cgcctatgcg ctatcgcgga tatattggtg caggtgtcgc aaaaatcttt
     1201 gatcatacag caagttatgc gggaatgaca gaacatgaat taaaagcatc aggcataaca
     1261 aattataaaa ctgtttttat cactcccttt gaccatgcct atttctatcc agaagctaca
     1321 agattaaatc taaagctgat ttttgatgca gatagcggtc gtattttagg tggacaagca
     1381 tttggagaaa aaggtgtcga taaacggatg ggagaacttt ctgtagcaat caccggaaac
     1441 ttgacagtct ttgatttgcc tgatttggag ttgccttact ctccaccgta ttctactacc
     1501 cgtgacccgt tgaatatagc tggttatgtc gcaatcaatc aaatgacgaa tatcgtagaa
     1561 acgatcaaag caagtgatat acccgaaaac gatttgaaag aagcgttctt tttagatata
     1621 cgtgaaccta ataaagcacc ttctggcagt atctcggcaa caaaaaatat tccaatgaat
     1681 gagttacgag atcgaatcaa tgaaatccca aaagataaaa aagtttacat tactttcaga
     1741 agaggattga atacttatac ttctgcccga atcttggcag gtttgggtat caaagcgatt
     1801 ttgattgaag aataaaaaaa cgaccgcggt gggtagccgc ggtctaggag ttatttttac
     1861 ttgaggagta aaaatgaaaa agtatatagg gttgttggtt ggtatgtata tagaatagaa
     1921 gataaatgtg aagaaattgt gcattatttc ttcacattta ttaggttata gtttaagaaa
     1981 gtttaaacat tgcgcaattt aattatttta gctgtttctt ttcataaaaa aacatgttgt
     2041 gactctgaga caaatcatag tccatagtga tgtctctaga gtaactacgc ctaatattac
     2101 gaggcatcat attaatctat cagcactagt acctaaacta gcatcccttt cgaatatttt
     2161 ttcttatata ataaagtttg ctttcactcc tctaatctca ccaaatcgac tggcttgcgt
     2221 tgtgtgatac taggaactga ttggtttaga acgacttttc tattgtaatc atatttttcg
     2281 ccatcctcgt tgtcttctaa tccttcaatg aatgatccct aatgtgtgta ttaaataatt
     2341 taattttttt gttcctattt atttaaca
//
LOCUS       DAZUMM010000156         2305 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000156 DAZUMM010000000
VERSION     DAZUMM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.166"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..1972
                     /locus_tag="ACG8E6_002665"
     CDS             110..1972
                     /locus_tag="ACG8E6_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5063017.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            1972..2304
                     /locus_tag="ACG8E6_002666"
     CDS             1972..2304
                     /locus_tag="ACG8E6_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5063018.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          828 a          342 c          453 g          682 t
ORIGIN      
        1 tctgtaatat tagttaaaaa gctataagtt gcacttagtt aagtgcaaac atttcatatt
       61 gtcgttagtt tagaaaacgc ttatattagc aatgtaagga gagatgataa tgcagcagct
      121 aacaaaaaga gaaaggcagc ttttgattta cttggtggaa atgtctgaac cggtaacaat
      181 caaaaagttg gctgaaaaag aaaatgttag tgttcggacg ataaaatacg atctagatga
      241 tataagagag tggctgaatg agcggaagca agtcctatat agcaaacgta gtcagggaat
      301 ttggatgaat attagtagct ctgatcgaat caaattaaaa agcgaattga tggatgttaa
      361 tcgttttgaa atatttgcag atcaaagtct ccgaatcaaa cgtttactaa ttatgctttt
      421 agtaacaaaa gaagctatta ctggaatgaa aatggccaac gcattagatg tcagcaaaga
      481 tacgatcatg aatgatttag atgtgctgga aaataatttt cttaaggaag gattgacctt
      541 gaatcgtcag gcgcgaaagg gtttttggat cataggagaa gaacgtctag ttcgattaac
      601 gattgaagag attcttcaaa aagagttcac ggattatgac atttataaat taatgtcatt
      661 gttattgaat ggcggtgaga ctgaatactt tgaaatgtat tctgctactg ctacacctat
      721 ccaagaggta tttaatcaag taatcattcg aatgcgacac ttactggaat ttgaaaatct
      781 agagaaactg aattacgcgg aattactaaa tattctgatt cgagtgacaa ttgctaccgt
      841 tcgcttacgc aaagaggcta cgattggtcg ttatcaattg gttgctgaac aagaattaga
      901 caaaaacgat ctttcttatc gcgtcatgca gcaggtcttt gattattatc agttgccact
      961 gttcgaggat gaatatcatt atatctatag tgatacattc gacacgattc accagcaaga
     1021 cgtcatgaaa ctagttgaga atgtgattta taaagtaagt aaagcaatca accattcttt
     1081 ttatcaagat ccacaattgt tgacgaatct gtatgctcat ttatccatgc gattatcgag
     1141 aaagaaaaaa tttgtcaatg aatataatcc ttttaaagaa gatatcaagc agaaataccc
     1201 ggaattattt gatgcgatca agcaagcagt tcgacaagaa ataagtgaac aaaatattct
     1261 gatcaatgat tctttcgtgg catatattgc cttacacttt ttggtttcct acgaaagaga
     1321 agaagatcta cgaagcgtta gagccgtcta catttgttca acagggttgg gtgtgactag
     1381 tctgatcaag caaaagatca atgaagaatt aaataatatc actatcgtgg catttgcttc
     1441 agtattgaat gcgcaagaaa tgattgctac caaagatcct gatttggtga ttagtatttt
     1501 tccaattaat ggcatcgatc gtcctttcat caaagtccat ccattaccca ctgaacagga
     1561 cttgaaaaaa attcaacaaa taataaaaaa aatacttgct tcttccaatc agcaggaaag
     1621 gcataacttg aatcttagag aaacgtccat caataaagat actttagaag actttagcaa
     1681 ggaattgatt ttgaaagctt atacgattta tgaacgttta cttgtcattt tggaagcaga
     1741 attgatccaa gaatatcgag aagctttttt gcttcatgta ttcttaatgg ttcatcgtat
     1801 cacatttgat caacaatata tggcagaagg tcgtcaagta gaccagatta ttttagacaa
     1861 aggaacactg gtaggaaaaa tcgaagcagt ttttgcagaa aataatttag gaatcaatca
     1921 gtcagaaatc agtgcattat tctcatatat aaaagaagga gcgtcaagtt agtgaaaatg
     1981 tcagacgagg ctttaaaaat tatcgaggaa agtcaaaatc gggaaaagtt aaaacaagtg
     2041 attgatctaa ttgaagagcg attagtagaa catgctattg taccaaccga gttgcagtgg
     2101 accattctaa ttaatcactt gaatgagatg ctgaaacgtt ctcaaagaaa ggaaacgatt
     2161 cccgtagtcg atcgggattt gttcaaagaa gtatccaaag aagccataac tatttctgat
     2221 gaaatagtta gaagtatcgg taatttaacc gatgatgaaa tatatgtttt gtcgattcat
     2281 tttgaaacag caaaaaataa ttaat
//
LOCUS       DAZUMM010000157         2297 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000157 DAZUMM010000000
VERSION     DAZUMM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.167"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACG8E6_002667"
     CDS             complement(<1..300)
                     /locus_tag="ACG8E6_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFS5063019.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACG8E6_002668"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACG8E6_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACG8E6_002669"
     CDS             complement(1014..1304)
                     /locus_tag="ACG8E6_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063020.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2204)
                     /locus_tag="ACG8E6_002670"
     CDS             complement(1512..2204)
                     /locus_tag="ACG8E6_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS5063021.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          657 a          433 c          335 g          872 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgagtttc tacatttgtt
     1681 ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct
     1741 gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt
     1801 tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt
     1861 agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga
     1921 tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa
     1981 tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct
     2041 ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc
     2101 tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat
     2161 agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa
     2221 tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa
     2281 ttacatatgt aatttta
//
LOCUS       DAZUMM010000158         2274 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000158 DAZUMM010000000
VERSION     DAZUMM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.168"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            16..672
                     /locus_tag="ACG8E6_002671"
     CDS             16..672
                     /locus_tag="ACG8E6_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5063022.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            672..2048
                     /locus_tag="ACG8E6_002672"
     CDS             672..2048
                     /locus_tag="ACG8E6_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5063023.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
BASE COUNT          912 a          258 c          402 g          702 t
ORIGIN      
        1 agttaggaga aaataatgga tatattagaa ataaagaatg tagcatatag ttatgcaaat
       61 tctaaagaaa aagttttgtc aggagtaaat caaaaatttg aacttgggaa gttttatgcg
      121 atagtaggaa agtcaggaac aggaaaatct acacttcttt ccttacttgc tggacttgat
      181 aaacctcaaa caggaaaaat attgtttaag aatgaagata tacaaaataa aggatatagt
      241 aatcatagaa aaaataatat atctttagta tttcaaaact ataatttaat agattattta
      301 tcaccaattg aaaatattag attagtaaat aaatcagcag atgaaagtat cttgtttgaa
      361 ctaggtttag ataaaaaaca aataaaaaga aatgttatga aattatctgg tggacagcaa
      421 caaagagtag ctattgctag agcattggta tcagatgctc caataatact agctgatgag
      481 cctactggta atctagacag tgttactgct ggagaaataa ttaatatatt aaagacatta
      541 gctaaagata gaaataaatg tgtaatagtt gtaacacata gtaaggaagt agcagattct
      601 gcggatatca ttttagaact aagtggtaaa aagttgaaaa aagtaaataa aatgaatttg
      661 gaggttgaat aatgataaaa aatgcatttg cttatgtaac tagaaaaagt ttaaaatcat
      721 taattatcat attagttatt ttatctatgt caactttaag tctcattagt ttatctatta
      781 aagatgctac ggacagagct tcaaaagaaa catttgctaa tataacaaat agtttttcta
      841 tggagataaa tagacaagta aatccaggaa cacctagagg tgggggaaat gtaaaaggtg
      901 aagatattaa aaagatatct caaacagata gtatagactc ctatgtaaaa agaataaaca
      961 gtgttgcaga tttagttgat tatgatatta ttgaaactca agagactctt gcgaatcaat
     1021 cacctgaaag agcaaagaat tttaaaagaa cagttatgtt aactggggtt aacgactcaa
     1081 caaaagaaac aaaatttgta tcagaagcat ataaattagt agaaggaaag catttagaaa
     1141 atgaagataa aaataaaatc ttaatgcata aagatttagc tgaaaaaaat aaccttaaag
     1201 ttggagataa gataaaaata aaatctaatt tatttgatgc tgataatgag aaaggtgcag
     1261 atgaaacagt agaagtagaa attaaaggtc tattcgatgg gcataatagc ggtggagtaa
     1321 gtgcagcaca agaactatac gaaaatacat taatcactga tattcataca gctgctaaag
     1381 tttatggtaa taccgaagat acagctgtat accaagatgc aacattcttt gtaaaaggtg
     1441 ataagaatct tgatagtgta ataaaagatc ttgggaaatt agatataaat tggagagaat
     1501 ataatttgat taaaagttca tcaaattacc ctgcattaca acaatctata tcaggtattt
     1561 actcaatttc aaataaatta ttttttggct cattaatatt tgcaggagtt gtagtttcat
     1621 tattattatt cttatggatg aatgctagaa agaaagaaat agcagtatta ctatcattag
     1681 gtatatcaaa attagaaatt tttggtcaat tcctaattga gatggtattt atatcaatcc
     1741 ccgcattcgt tggttcctat ttcttagctc aatatacagc tgacaagctt gggaataata
     1801 tattgaataa ggttactggt gatatagcta aacaaatagc taaacaatct gcatctagcc
     1861 aattaggtgg tggggcagaa gctgaaggat ttaacaagac attatcaagt ttagatatca
     1921 atgtattacc taaattcatg atttatgtag ttatatttat gagtttagta cttcttgtat
     1981 cattgattat ttcttcattc aatatattaa gaaggaatcc aaaagaatta ttaattgaca
     2041 ataattaaaa ttttcggtgc tttttagcac caaaaatgag agtgtaatat aaaccgtgta
     2101 agtaaccatc aatcctaggc gggtggttgc ttcacggttt ttatttttct aaaataagat
     2161 caagaggtga ttacacgatg gctagaaaga aaagaaatcc tgatgctgag aagttagctg
     2221 aatccatttt gaatacttat caacccgaat ctgtagagga tatgcaagat gttt
//
LOCUS       DAZUMM010000159         2273 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000159 DAZUMM010000000
VERSION     DAZUMM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.169"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..240)
                     /locus_tag="ACG8E6_002673"
     CDS             complement(<1..240)
                     /locus_tag="ACG8E6_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5063024.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRT"
     gene            851..1075
                     /locus_tag="ACG8E6_002674"
                     /pseudo
     CDS             851..1075
                     /locus_tag="ACG8E6_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1335..1589
                     /locus_tag="ACG8E6_002675"
     CDS             1335..1589
                     /locus_tag="ACG8E6_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5063025.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            1590..1907
                     /locus_tag="ACG8E6_002676"
     CDS             1590..1907
                     /locus_tag="ACG8E6_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5063026.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          777 a          365 c          421 g          710 t
ORIGIN      
        1 agtgcgatac tcagacacat cattcagtaa caaccgaacg gttgtcgtcc cccaacgaat
       61 gatcctagag gggacgacac aaagacaatg ggaacactct tctggcttgt acagaagagt
      121 tcctttgtaa atcaagcaaa tttgcccttt gtatttttct ttagttaaac aattttcctc
      181 gaaatagata tttaggtcta gaatatttag ggtatctttg ataagctgag tatgagacat
      241 cttgaatcct cctatgaatt ggtagccaaa ttaattctaa aggatttcag ggtgtctttt
      301 ctatattttt ttatacaaaa aagatgttgg tgaagttcac caacatcaaa aagtatagag
      361 cctttcatta tatcaccaaa ccccatcagc gtgcaggctg atggggtttt tttacgtttc
      421 tatattatag aattaacggg taggcttact gtcttttttg gcaattttgc aatcaagcca
      481 tttgcagagg taactgcaca ctacatctgc aaatggcttg attgcaaaaa caaaatcaaa
      541 aaggtatttc attatatctc ctcctcccct tgacagatta ggccggggta gcagacagca
      601 aaactattat aacataactg aaatacctaa atgacatata cagatatgat aatttatgta
      661 ttgatttttc taacaaaagc acatatttta atatatacta cctattttag gtagtatata
      721 tttttagata caatatcgat cagttttgag gaaatgaaat tctagatatt ttttgctaaa
      781 tagagttact ttcaatattt tttaacgtaa ttaggcagaa gtcactcatc ctctataggt
      841 ttgtgatgaa attgccattg atccatttaa gtaagagcaa aaaactggag gtttatcagg
      901 taattggtct aatggattta agtatgcggt caccgtgtat agagttgctt atgagattca
      961 agaaaataaa gttattccag tattgctata tggaacacat aacaattttt atgaattagc
     1021 cgagaacact cgcaacttta ttcgtgtaga tgaatcggct tcggttgagt tataacttta
     1081 gctatatcaa tcaaccgacc gacatatgtt aaatctatat ataataaaaa tacctactta
     1141 aatggatgtt aacggaagtg tatggtgagt agttcactcg tattttgata tcgtttgtat
     1201 atgttttcag cgttctaagt ggcttatttt gtccaataat aatatggagt ggaatccctt
     1261 aagaaacacc gttggcaatt gttgttataa catgttataa tttattcatc aaaaactaaa
     1321 aggagtgtga taacatggga aaagaactaa aggttagaaa gatcggcaat agcgtgggag
     1381 tgatccttcc aagctctctt ggattaaaaa gcggagacac tatccaagcc aaacaagaag
     1441 gcaacttatt catattagat actactcaaa tagcaaaaga acacgatcgc aaattgattg
     1501 aagaaagttt ccaagacttt gagaaaggtt taacagtttc agaaattgaa atggtaaaag
     1561 ctttcggaaa atacggctgg agtgaataga tgaaagagta cacgctagtt tattctaaaa
     1621 gtttccagac cagccttgaa acgataatag aagaatggga aaatgagctt gctctgtctc
     1681 cagccagcat aaaaagattt gtaggtgcga tccagaaggc tttagaacta accaaaaatt
     1741 ttccaactat gcatgaggaa gtttccgaaa tctatggaat ggatatccca acatatcgga
     1801 tcgtgatcgg caaaagctat gccttatttt atcggatcga ccatgaaaaa aaagagatac
     1861 ttgtaggacg tattttccaa tctaaacaaa tgaaactagg cttttaacaa taattaggca
     1921 aaagtcgctc atcctctaca ggtgggtgat taatgccgct cgatatggtt gttaaccgtc
     1981 ggttactcga agattgatat ataagactta gtaccttttt attttgctcc atgttatggc
     2041 cgaaggtcta aagatacaat cattagtcta gagaaaaatt aagaaccgta ggaactacgg
     2101 ggatcgcttg gtgaataaga gacactgcta ttagtgaaaa aataagatat caagtacgct
     2161 ctattcccaa aaatttcgtg actttactca tgagaggttc aagtagtata tttttaatca
     2221 aaattgacgt aacttttaat aaatactacc taaataaggt agtatttatt ttt
//
LOCUS       DAZUMM010000160         2221 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000160 DAZUMM010000000
VERSION     DAZUMM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.170"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..1164
                     /locus_tag="ACG8E6_002677"
     CDS             52..1164
                     /locus_tag="ACG8E6_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HFS5063027.1"
                     /translation="MKHISVLIKPASSLCNLRCSYCFYANVSSLREVRSFGKMKEEVT
                     EKMIENIYADLEDGDQLTLAFQGGEPTMAGLNYFRTVIRLVAQQQKQVQVHYAIQTNG
                     TMINEKWCAFLKEHDFLVGLSIDGHPLYHDLHRVDPKGRGTFQRVLQTKQLFDYYEID
                     YNVLCVLTNPLAKEAKKVFRFLKEQQIDFVQFIPCLDDLDAKKRNSYALTPKRFAGFY
                     HQLLQLWLQELAEGHYISIKLFDDLLNLLIKRQVTACGILGNCQVQYVIEADGSVYPC
                     DFYVLDEYRMGYIQEQTLRQLFEQDIAKTFLCGRPEMPKKCLSCPFQKMCRGGCKRMK
                     DAIYVDNTGYCGYQQLLQEFVPQINRILGLLQGVSV"
     gene            1161..1937
                     /locus_tag="ACG8E6_002678"
     CDS             1161..1937
                     /locus_tag="ACG8E6_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFS5063028.1"
                     /translation="MIGFIYFIVIVLANTVGAVSGMGGGVLIKPIFDFIGAHSVAAIS
                     FYSTVAVFTMSLVSTARQLASGRKIHWQIVLWVSGGAVLGGILGNVAFDHLLLLFQNE
                     DEVQMIQIFLTVVTLLFAFFYTKYDWPGFQLKNNFWYCFCGLVLGFLASLLGIGGGPI
                     NVSLLMLMFALPIKEATVYSICTIFFSQLAKIVTIAGTTGFGLYDLKILWFVIPAAIV
                     GGLLGAKVSTVLSPQKVTLVFQTVIFLVLIINLYNGYQLL"
     gene            complement(2002..2132)
                     /locus_tag="ACG8E6_002679"
                     /pseudo
     CDS             complement(2002..2132)
                     /locus_tag="ACG8E6_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031600262.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          603 a          422 c          494 g          702 t
ORIGIN      
        1 gttatagcaa tttttcataa ttaaactatg ttaaaaaatg aggttcttac tatgaaacat
       61 atttcagtat tgatcaaacc cgcatcttcc ctctgcaatc ttcgctgcag ctactgcttt
      121 tatgccaatg tcagttcctt gcgagaagtc cgctcgtttg gcaaaatgaa agaagaagtg
      181 acagagaaaa tgatcgaaaa tatttatgca gacttagaag acggcgatca attaacatta
      241 gcgtttcaag gtggagagcc aacgatggcg ggcctcaatt attttcgtac agtgatccgg
      301 ttagtagcgc aacaacaaaa gcaggtacag gtccattatg cgattcaaac gaatgggacc
      361 atgatcaatg aaaaatggtg tgcttttttg aaagaacatg attttttggt cggtctctcg
      421 atcgatggcc atccgctcta ccatgatctg catcgggtcg atccgaaagg aagaggaact
      481 tttcagcgag tgctgcaaac aaaacagctg tttgactact atgagatcga ctacaatgta
      541 ctttgtgtct tgaccaatcc gttggcaaaa gaggctaaga aagtctttcg ctttctaaaa
      601 gagcaacaaa ttgattttgt ccagtttatt ccttgtttag atgatctaga tgcaaaaaaa
      661 agaaatagtt atgctctcac accaaaacgt tttgcaggat tttaccacca gcttttgcag
      721 ttatggctgc aagaattagc ggaggggcat tatataagta tcaagctttt tgatgatctt
      781 ttgaatctat tgatcaagcg acaagtcact gcctgcggga ttttaggaaa ctgtcaggta
      841 caatatgtca ttgaagcaga tggcagtgtg tatccttgtg atttttatgt gctggatgag
      901 tatcgcatgg ggtatattca agaacagact ttacgtcagt tatttgaaca ggacattgca
      961 aaaacgtttt tgtgtggacg ccccgaaatg ccaaagaaat gtctgagttg cccgtttcaa
     1021 aaaatgtgtc gtggcggctg caaacggatg aaagatgcca tctatgtaga taacacaggc
     1081 tattgtggtt atcagcaact tttgcaggaa tttgtaccgc agattaatcg gattttaggt
     1141 ttgttacagg gggtaagtgt gtgattgggt tcatttattt tatagtgatc gtgttggcga
     1201 atacggttgg tgccgtttca ggcatggggg gcggtgttct gatcaaaccc atttttgatt
     1261 ttatcggtgc ccattctgtt gcagccatct cgttttattc taccgtagcc gtgtttacca
     1321 tgtcgctggt ttcgaccgca agacagttgg ctagtggacg taaaatccat tggcaaatcg
     1381 tcctttgggt ttcaggcggc gccgtcttag gaggcatctt gggcaatgtc gcttttgatc
     1441 acctgctgct tttatttcag aatgaagatg aggtacaaat gattcagatc tttttgaccg
     1501 ttgtgacact gctgtttgct ttcttttata ccaaatacga ctggcccgga ttccaactga
     1561 aaaataactt ctggtattgc ttctgtggcc tagtccttgg ttttctagcg agtctgctgg
     1621 gcattggcgg tggaccgatc aatgtgtcct tgctgatgct gatgtttgct ttacccatca
     1681 aagaagccac ggtctattcg atttgtacga tttttttctc tcaattggcg aaaatcgtga
     1741 cgattgctgg gaccactgga tttggcctct atgacttaaa gattttgtgg tttgtgattc
     1801 cagccgcgat tgttggcggt ttgttagggg caaaagtcag cactgtcctc tcaccacaaa
     1861 aagtgacgct ggtcttccag acagtcatct tcttggtgct aatcatcaat ctttataacg
     1921 gctatcaatt actataaaaa acgagtatta tcaaccactt atagaaaacg attaattagt
     1981 aactttagaa gtactttctc attaagaagc gctctttttt gtgacttttc tgaaactaga
     2041 ataaatactc tcaattgcat tagtaatatg catgattttt ctaatagcac tgcctgttca
     2101 ccatgtgcaa agtaccgttt ccagacatct actaacaaaa taggctcttt gtcaataagg
     2161 actgatgagt tgtgcaaaat caaatctggg tcagaatgaa ccacattctg gctcagattt
     2221 t
//
LOCUS       DAZUMM010000161         2140 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000161 DAZUMM010000000
VERSION     DAZUMM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.171"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..567
                     /locus_tag="ACG8E6_002680"
     CDS             <1..567
                     /locus_tag="ACG8E6_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063029.1"
                     /translation="EEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETE
                     KYIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATID
                     DYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTY
                     TYDDTEEIQNELDRKRMPYQRFERKKRQ"
     gene            complement(614..850)
                     /locus_tag="ACG8E6_002681"
     CDS             complement(614..850)
                     /locus_tag="ACG8E6_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063030.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
     gene            948..1127
                     /locus_tag="ACG8E6_002682"
     CDS             948..1127
                     /locus_tag="ACG8E6_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063031.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            1258..1527
                     /locus_tag="ACG8E6_002683"
     CDS             1258..1527
                     /locus_tag="ACG8E6_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5063032.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            1520..1777
                     /locus_tag="ACG8E6_002684"
     CDS             1520..1777
                     /locus_tag="ACG8E6_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS5063033.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          787 a          299 c          429 g          625 t
ORIGIN      
        1 gaagaacaat tgtatttgga aagtgaaacg agtcaaacga aggtcacagt cagccataat
       61 cgtttagacg tgtataaacg tcgtcctgaa acggtaaaaa aaggaacgga gcttctacga
      121 ctagaaacag aaaaatacat catgaatgtt tacctagaaa ggagtgagct ggtcgaaaac
      181 tttgtcagct acacgatttg caggaaacac tattactcag aatcagaata ttattcaatt
      241 tttgagagca tggaattaat ggaagaagaa atggaagagc taaacagcaa agatgcgacg
      301 atagacgatt attaccttgt atttactaga tttttgacgt gtggaacgga atctatcgtg
      361 ttaatgggaa tggattatga actcgctgta cccgaagcat ttttgaggga atatgtagaa
      421 gaagtagata acccaggcga cgactggtac acgactttaa ctgatttttt agacacctat
      481 acttacgacg atacagaaga aatccaaaat gaacttgacc gtaaacgaat gccatatcaa
      541 cgatttgaaa ggaaaaaaag gcaataatca tttataccga aaagtgagaa tgtgtgaaat
      601 aaggacaaaa aacctagtaa ctaagggaat ggttcttatt tcgcaccaat cgctccaacc
      661 gcccccgccg cttaggtggt gcgaaataag cgggcaacac ccgataacct aagagaaaga
      721 aggagatcca tatgaataaa atcattcctc tattgttagt cctatttgtt ggcgaaatac
      781 ttatgcgcca tgctgcagct gtgcatttaa acttcgtagt atttttggca atactggtat
      841 tttgtttgat cttttggcgc tttgtattag cccttgtgag tctactattc aagatgattg
      901 cagccatatt tttcataggg ttatttcgat gaaaggagag agtactaatg ccaattgcaa
      961 taggaaataa acggttaccc gttacgttgg atgaaaagag acaaaaagaa ttgcagcaac
     1021 taaagcagaa gtacggcaaa agtgaatcca ggattatgtg tattgcgtta gatttattga
     1081 ttgcccaaga aaaagcagga tttgaggtac cagcactcaa aaagtgacgt cactttttat
     1141 cctaaaaact aaaagtgata gcacttttaa ttataagaag ttagaatatt aatcatttgc
     1201 ttaattgtac aatataatgt acaattgttt tatagaaata aataaggggt gaaaggaatg
     1261 gaagcagtag cttattcaaa tttccgccaa aatttacgta gttatatgaa acaagttaat
     1321 gaggatgctg aaacacttat tgtaacaagt aaagatgtag aagatacagt tgttgtatta
     1381 tcaaaaagag attatgattc tatgcaagaa acgttgagaa cactttctaa taattacgtc
     1441 atggaaaaaa ttcgtcgagg agatgaacaa ttctccaaag gtgcatttaa aacacatgac
     1501 ttaatcgagg ttgaatctga tgattaaggc ttggtctgat gatgcttggg atgattatct
     1561 ttattggcat gagcaaggaa acaaaagcaa tataaaaaag attaacaagt taataaaaga
     1621 tatcgatcgt tccccctttg ctggattagg aaaacctgag ccattaaagc atgatttatc
     1681 tggaaaatgg tccagaagaa ttacagatga acatagactg atatatagag ttgaaaatga
     1741 aacgatattt atttattctg caaaagatca ctattaacca atcggaagta aggaaagggt
     1801 cagaaactta aaagtttttg atccttattt tatttaccct agtcatttaa aaagctaata
     1861 tagcttagtg ttgattgtta ttaatgaatg tgtttgttac gcgtattacg gatataaggt
     1921 tagtaaaatc atttctaaag ttgaggaaaa gtaaatataa atggcttaaa tttcaacaat
     1981 ttgaagttga atagatatgt tataatacta ttgtagtgtg ggatgttagt tactaaagga
     2041 tgacgcttat atatatgact gaatagaata agcaataggt ttaataatct attttaaatt
     2101 ttttgtacta gttttagtta attagcaaaa acaacaaaaa
//
LOCUS       DAZUMM010000162         1913 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000162 DAZUMM010000000
VERSION     DAZUMM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.175"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..271)
                     /locus_tag="ACG8E6_002685"
     CDS             complement(<1..271)
                     /locus_tag="ACG8E6_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFS5063034.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCG"
     gene            complement(494..1525)
                     /locus_tag="ACG8E6_002686"
     CDS             complement(494..1525)
                     /locus_tag="ACG8E6_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5063035.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(1532..>1913)
                     /locus_tag="ACG8E6_002687"
     CDS             complement(1532..>1913)
                     /locus_tag="ACG8E6_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5063036.1"
                     /translation="LINLKELVDAAETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSS
                     VFGEYMLTNLLIGGRFQTVRIYMLRRMNENGHLASAVMVLYLLFMVGIALTTFMLTYR
                     QKQQLSLKQTREEKQSKKKPLKEL"
BASE COUNT          569 a          462 c          336 g          546 t
ORIGIN      
        1 ttccacaatc atggcagaga aaacgggtac gttttaattc taaaatagtt aacttttctt
       61 tgactttcaa catctgagtg cgtgtcgtat aggaaccgtt gcggataata cgaaccgatc
      121 gacagtgttc acaataggaa ggagagtcat ccaattttcc tttaataaac tgcgcttgaa
      181 cgcctttata ttctttttca atcagccatt cttcatcaaa aaataaatgt ttatctgtta
      241 atccgagtaa ttttctagta tgattatcca ttgagagact cctttggtag gatgacgttt
      301 tggtcgacta aattttacca aaagtgagtc tctttttgag ttgaaacgaa gaaaaagtgt
      361 tagtagaaaa tcgccttcga ttttccacca acacaaaata ttatagaacc aaaatcaatt
      421 ctaccttaga tgttcttata tcactctatc agtcctattt gacaaagagc cagaaatgtt
      481 tgccgactat acactagctt tgttgtgcaa aatgctgagt atcaatttcc acagggattg
      541 ctagaatatc tttcagctga atgtataagg catcgctaat tttctcttcc cgatcgttca
      601 accgatccac taaaatttct ttgccctcca gcgtttgaaa aacataccgt tgaatgttgc
      661 ctaaaataaa tgattccttc catagaaccg gaatcttaac cccctgagga accggttcaa
      721 attgaatcag ctccggtctt aaataataaa tgcagcgatt gcccttagcg gaaaactcgt
      781 gggtatactg attcagctca taccattcga aacgattata gttgccgata aattccgcaa
      841 caaatcggct ctgaggattc tgatacaatt ccttcgaggt ggaaacctga gccacccgcc
      901 cagcttccat cacaaaaatc cgatcactga ttcgcatcgc ctcttcttgg tcatgagtca
      961 caaaaatcat cgtcatccct agcgattgct ggatagcgcg caaatcccgc tgcagctgtt
     1021 ttctaattcg ggcatccaaa gcacttaatg gctcatccag cagcaggact ttcggttcag
     1081 tgaccaatga gcgagccagt gccacccgtt gtcgttgacc gccagaaaga aactggacat
     1141 ttttatctgc ctgctccgtc atatccacca atgatagcat ctcaaaaacc ttttcctgaa
     1201 tcacctcagg actcagcttc tttaccttca aaccaaaggc tacattttca aaaacagtca
     1261 tagttggaaa caatgcatac tgttgaaaaa caaagccgat attgcgatcc ttcgtaggct
     1321 tatcggcaat attctcattg tccagcacaa tctcaccgct ctttggttgt tccaatcccg
     1381 tgatacagcg cagcaacgta gactttccgc acccgctggg acctagtaaa gtaaccagct
     1441 cacccttttc aatctgaatt tcatcaatag ctaaaatctg tttttcatca aaaaacagat
     1501 tgagatcatg tatttctaaa aacattttcc gtcacaactc ctttaagggc ttctttttcg
     1561 actgcttctc ttccctcgtc tgcttaaggg acagctgttg cttttgtcga taagtcagca
     1621 taaaggtagt taaagcaatt cccaccataa acagcaaata caaaaccatc acagcgctag
     1681 ccaaatggcc gttttcgttc atccgccgca gcatatagat gcgtaccgtc tgaaatcgac
     1741 cgccaatcaa caggttcgtc agcatgtact ccccaaaaac cgaagaaaaa atcatcagag
     1801 aaactagggt cacaccgata cggatgtttg gaaacagcac ctggataata atcgtagaca
     1861 tcggggcgcc caaggtttct gctgcatcca ctaattcctt cagattaatt aag
//
LOCUS       DAZUMM010000163         1885 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000163 DAZUMM010000000
VERSION     DAZUMM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.176"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..1097)
                     /gene="queA"
                     /locus_tag="ACG8E6_002688"
     CDS             complement(66..1097)
                     /gene="queA"
                     /locus_tag="ACG8E6_002688"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFS5063037.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1290..>1885
                     /locus_tag="ACG8E6_002689"
     CDS             1290..>1885
                     /locus_tag="ACG8E6_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5063038.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT          541 a          394 c          350 g          600 t
ORIGIN      
        1 ttttctttgt cataaaatct ttgtaaagct tttttgttat tcaaaactag cggcttttca
       61 taaccttatt tcacgaacat cgcatctcca aaactaaaga aacgatagcg ctcttcaatc
      121 gcatgctggt aagcagataa agtcaattct cttcctgcaa aggcactaac taacatgact
      181 aaagtcgatt ttggcaaatg gaaattagtt gagaaagctt ctaccacttt gaattcatag
      241 ccaggcgtaa tgaaaatatc agtccatccg ctgtctgctt tgatttctcc attaaatttc
      301 gttccaatcg tttctaatgt tcgaattgaa gttgttccga cagccacgat tttcccgcca
      361 tttttacgta cttggttcaa tcgctcagcc gcttcttccg tcaaacggta gaattcacta
      421 tgcatatggt gttcttcaat tttatctacg ctgacaggtc ggaatgtacc taaccctaca
      481 tgtagtgtca gatagactaa ttcaacaccc tttgcttcga tctcatcaag taattctttc
      541 gtaaaatgca atccagctgt tggcgctgca gcagaaccat tttctttcgc atagacagtt
      601 tgatagcgat ctgggtcttc cagacgttct ttgatatatg gaggcaatgg catttctccc
      661 aatgattcaa gaatctctag aaaaattcct tcatactcaa agtcaatgat ccgtccgccg
      721 tgttccaatt cttctttcac tactgcttgc aatctgccat caccaaaaga gattcttgtc
      781 ccaactttgg cacgtttcgc aggtttgatc aatgtctccc acgtatctcc ttgtgtattc
      841 gttagaagaa ggacttctaa atgcgcacca gtatctggtt tttctccata taaacgagca
      901 ggcagaactc gtgtattatt cataactagc gcatcaccag gctgtaactc ttcaatgatt
      961 tcatggaaat gtttatcttc catctctcca gttttccgat ccaaaacaag taaacgagaa
     1021 tgatcgcggt cttttaatgg tgtttgtgcg attaattctt ccggcaagtc aaaatcaaaa
     1081 tcttctgttg ttaacatatc gtcactcact ttctatcatg tgcttcatca ttctatcatg
     1141 tttttcgttc tgttggcaaa atcagcactt tttcacttct attttttgtt ttcctaaata
     1201 aaaaaactac cctatttgta ttttttcatt gttttaggca tactagtaat agatagttct
     1261 tcattgaaaa tagaaaaaga ggtggcacca tgattgaatt tcaaaatgtg tcaaaaattt
     1321 ataaaggtgg aaaagtcgct gtagaggacg tcaatctttc atttgaaaag ggtgagttca
     1381 tttgttttat cggtacgagc ggaagcggaa aaacgacgac gatgcggatg atcaaccgaa
     1441 tgactgatcc aacacaaggg aaaattttga ttgatggaaa atccatccaa gaaattgatc
     1501 cagtggaatt gcgacgtcag atcggctatg ttatccaaag tattggattg atgcctcaca
     1561 tgacgatccg tgaaaatatc actcttgtcc aaaaattgtt gaaagtcagt caagaagaac
     1621 ggaataaaac agctgaaaaa atgattgatc ttgtggaatt gccccgtgag atgttagatc
     1681 gctacccaca tgaattatct ggtggtcagc agcagcgtat tggagtcgtt cgtgcccttg
     1741 cagcgaatca agatatcatc ttgatggacg agccattcgg tgcgcttgat ccaatcactc
     1801 gtgattcctt gcaagacctt gtcaaagatt tacaagagcg tttgggaaaa acgatcgtat
     1861 ttgtaaccca tgacatggat gaagc
//
LOCUS       DAZUMM010000164         1863 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000164 DAZUMM010000000
VERSION     DAZUMM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.178"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1863)
                     /locus_tag="ACG8E6_002690"
     CDS             complement(<1..>1863)
                     /locus_tag="ACG8E6_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFS5063039.1"
                     /translation="IPPAIFFYYSILFDSRFKTLQQNRNSNQALIFALQAELGYSPSE
                     ATGSYGNGTTSKTYPVSEGNSGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAF
                     RKFMVLPEYTEIADVVVIKGLLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVG
                     RYLTGTVGIGKDERNKYLTTEELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKK
                     AIDAAKNLNIPLTTVIYFAVDLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRN
                     VCSQIINNGYASFAFVSDMSTGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIA
                     TSGRDSGFNELSNDTNNDNYIAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYV
                     NYSGLSWDIISSPVTEHDRKIYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQ
                     SQMFLADTINDSVSDFAGWLGDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDL
                     YQDVDAVNVYTLAKRNSDSTALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNS
                     LLENDETVDILGVGIISFFIDKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQF"
BASE COUNT          636 a          374 c          244 g          609 t
ORIGIN      
        1 aaattgaata attttttcag aaaatccatt tgcaactttc ccctcatctc caataaaatc
       61 aagataagta aaatctgtcc caagtgaatt attaactatt ttatcaatga aaaatgaaat
      121 tatccctaca cctaagatat caactgtttc gtcattttct aataaactat ttgttactga
      181 agcgatttta tctgaagaac caaatctgtt cttaacaaat tgattaaatc tatcaatagc
      241 acccttatca caatagtacg tttttaaagc cgataaagct gttgaatcag aatttctttt
      301 tgctaaggta taaacattta ccgcgtcaac atcttggtat aaatcttcta gactaaatgt
      361 tccacctgca gccgtaccta ctaatgcaaa tacaccttgc tccatttgta ttcctaattt
      421 cttaacttca ccccagcaag tcattaaatc tcctaaccaa cctgcaaagt cagaaacact
      481 atcattgatt gtgtcggcta gaaacatctg tgattgtaac gtcacaatta agtgaggcaa
      541 acctattatt gaaccagtat taggatctat aaaataatta tacaggccct cgctattagt
      601 taatttattc ctatattcat cataaatttt tctatcatgc tctgttacag gagaagaaat
      661 gatgtcccat gacaaaccag aataatttac atatctataa aatgtcaaat gagggttcca
      721 aggattatca agaccagctt gtgatagata ttgatacgca cgtaccatct gatttataac
      781 cttctgattg tatttggcaa tgtaattatc attattagta tcatttgata actcattaaa
      841 tccgctatct ctacccgatg tagcgattaa atcgatacca acagctccgt tgccacttcc
      901 tatggtaaat tcaacaaatt gatcaaaaga ccattgtcta ggcataggaa aacctaaatt
      961 cccactataa ccagtcgaca tatctgaaac aaatgcaaac gaagcatacc cgttattgat
     1021 tatttgagaa caaacatttc ttgttccgta aacacccgtt tgatacccgc tatatgacat
     1081 tacagcagat acccccttaa aatattctcc agcatatgca agaatttctt cccctaacat
     1141 gtctagatca acagcaaaat aaataactgt tgttaatggt atatttagat tttttgctgc
     1201 atctatagcc ttcttaccat ctgataagcc acgatcataa gtaaaataag ctaatgctgc
     1261 acctccatct tgatagatag gaaacacaga taaattttta gaaaaaatat tattcaattc
     1321 ctctgtagtt agatacttat ttcgttcatc ttttccaatt ccaacagtac cagtaagata
     1381 tctaccaact atttttatat cattatcaac caatgtttgg atttgagatc tagttaattg
     1441 cgtagaagta tctgcgccat ctgcagatct tctaatatct ccagcgctag acaatagtcc
     1501 ttttattaca acaacatctg ctatttcagt atattcaggc aataccataa attttcggaa
     1561 tgccagtact tctaaaccca tatatttagt gaaaatacca tcgaatgtac cgtcttcaaa
     1621 ataaccatta acatataatc catattgaat tattcttaca tagtttcccg agttaccttc
     1681 agacacagga taagttttac tagttgtccc attaccataa gaccctgttg cttcactggg
     1741 agaatatcct agttcagctt gtaatgcaaa aattaatgct tgatttgaat ttcggttctg
     1801 ttgcaaagtt ttaaatctac tatcaaataa gatagaataa tagaaaaaga tagcaggagg
     1861 aat
//
LOCUS       DAZUMM010000165         1831 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000165 DAZUMM010000000
VERSION     DAZUMM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.179"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..614)
                     /locus_tag="ACG8E6_002691"
     CDS             complement(<1..614)
                     /locus_tag="ACG8E6_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063040.1"
                     /translation="MTETKFIQDPWSKMTTRNGLRGDEAISALQKSIRRGKEKEACEF
                     AYEMYITSPQFEEKLWRRLLAISVEDVGMGNPMAAIMINNLNAMRKEFQYNESDRAMY
                     FFHAIRYLCQSEKDRSSDLLKNIVIKSFAMGYIPEIPDIALDKHTVRGQAMGRGSEHF
                     LHEASKVIPQAKVDNDYKEQYEKIIETYDPDHVVPSTFSFNPWQV"
     gene            655..1347
                     /locus_tag="ACG8E6_002692"
     CDS             655..1347
                     /locus_tag="ACG8E6_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5063041.1"
                     /translation="MDVEMLIDKYQLNDSEAEVLRFMKKNRQSLKNIGIRDVAKASYV
                     STATIINMAKKIGYSGYSELVFALGNSQSDITMPSTFSNEEKEAFIHLMSAYRDKRIM
                     ILGSGFSQNLANYFSESLNLYGFRATANSHLEFLRQQVQQDILLFIISNSGDTVRLAE
                     LAKIANDHFIDVIAFVGQKNSKIGTLATLTISTETYIPQKISDYQPNLFFGTTLNQFE
                     LLLSAALKAIYK"
BASE COUNT          559 a          345 c          328 g          599 t
ORIGIN      
        1 acttgccaag gattaaatga aaacgtactt ggcacgacgt gatctggatc ataggtctcg
       61 atgatttttt cgtattgttc cttgtagtca ttatcaactt ttgcttgggg gatcacttta
      121 cttgcttcat gcaaaaagtg ttctgaacca cgccccattg cctgtccccg aacagtatgt
      181 ttatccaacg caatatcagg aatttctgga atatatccca tagcgaaact cttgataaca
      241 atgttcttaa gtaaatccga tgaacgatct ttttcagatt ggcataggta acgaatcgcg
      301 tggaaaaaat acatcgcacg atcggattca ttgtattgga attctttgcg catggcgttt
      361 aagttattga tcatgatggc ggccatagga ttgcccatgc ccacatcctc aacactgatc
      421 gccaatagcc ttctccagag tttctcttca aattgtggtg aggtgatata catttcataa
      481 gcaaattcac atgcttcttt ttcttttcct cgacgaatcg atttctgtaa tgctgagata
      541 gcttcatctc cacgtaagcc gttacgggta gtcatttttg accacggatc ttgaataaat
      601 ttcgtttctg tcataatata atactcctat tagataaaat ttgaggtgaa tcttatggat
      661 gttgaaatgc ttatagataa ataccaactg aatgactcag aagctgaagt cttacgattt
      721 atgaaaaaaa atcgtcagtc tttaaaaaat attgggattc gtgacgttgc caaagcatcg
      781 tatgtttcta ctgcaacgat tatcaatatg gcgaagaaaa ttggatattc cggttatagt
      841 gagttggtat ttgctctagg aaacagccaa tctgacatta cgatgccaag cacattctcc
      901 aatgaggaga aggaagcatt catacattta atgtctgcgt atcgcgataa acggattatg
      961 attcttggct caggtttttc gcaaaatcta gccaattatt tttcggaatc tcttaatcta
     1021 tatggatttc gtgcgacggc caatagtcat ttagaatttc ttagacagca agtgcaacaa
     1081 gatattctgc tttttattat ttccaattct ggtgatacag ttcgattagc cgaattagct
     1141 aaaattgcga acgatcactt tatcgatgtg atcgcatttg ttgggcaaaa aaattcaaaa
     1201 atcggtacgc tggcaacatt aaccattagc acggaaacct atattccaca aaaaatatct
     1261 gattaccaac caaatctttt ctttggcacg acactgaatc aatttgagct tttgttgagc
     1321 gcggcgctga aggctattta taaatagaag aacgctgtgg ccactgtttc ttcaaaatct
     1381 agtataaaga atcttcttta gaatgtaatt gtttcctgtg tgttctgaac atgtttagga
     1441 atttccaaac ttgtttagaa aactttctca ttccctccgc tgtagaattg aactcacata
     1501 aaaaacatct aaaccaaaat atctacaagc ttggaaagga taataggttg ttattaaaaa
     1561 taatttttat tcctgaacct gaataattca tagctttaat tctttggcac attttattga
     1621 aatatgtatc actaatatcc ctatcaactg attttgacta aaaagttact tcaaacattc
     1681 actgttcaaa aatattgagg aataaatttt gggtgtaagg cattttgagc atacttctcc
     1741 ctggataact tcccagtaaa aaaatcgtta gattgttgga ttagatccat tgactgcctc
     1801 gcctcaggcg agcaaagacc ctgtagaatt c
//
LOCUS       DAZUMM010000166         1783 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000166 DAZUMM010000000
VERSION     DAZUMM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.180"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..241)
                     /locus_tag="ACG8E6_002693"
                     /pseudo
     CDS             complement(1..241)
                     /locus_tag="ACG8E6_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(248..1711)
                     /locus_tag="ACG8E6_002694"
     CDS             complement(248..1711)
                     /locus_tag="ACG8E6_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HFS5063042.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQFAYSAHTSCDDNGFILSTIITPGNIHDSQVAFQLVKQSKRLFPEIN
                     CVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQ
                     MLAFSTVTREGYREYKSNPKECVNCPLLNQCTKSKNHQRVITRHVWGDLMDEVEHLRL
                     TDLNKSIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATW
                     LWKGKEPLFFCSKIRNEVDKKLFQARVTSLEQLLSTV"
BASE COUNT          519 a          362 c          320 g          582 t
ORIGIN      
        1 ggttccacca attgacatag tcaaataact ccaaatccaa ttgttgtaag gtttcaaatg
       61 tgtattgata gacaaattct actttcaacg acttataagt tgattcagct acggcattat
      121 caaaaggaca gcctttatga ctcaatgatc gattgatgtc aaaagttgtt gataattcat
      181 caatagcttg gttatcaaac tcttttccac gatcagtatg aaaaatctca acctctgtca
      241 gaggttgtca gactgtagac aaaagctgtt ccaaactcgt aactcgagct tggaacagct
      301 ttttatctac ttcgtttcga atttttgaac aaaaaaataa aggctccttg cctttccata
      361 gccaggtggc taatttcttt aaattcattg cagcaaacac tagcatcgta tgcgtcgcga
      421 ctttttccaa gcctcgatac ttggtccaac gcatcccatg cttttctttt gcatccgcaa
      481 atattcgttc aatcgtttgt ttccgttttt tgtaaattga tttattcaga tctgttaggc
      541 gaagatgttc cacttcatcc atcagatctc cccaaacatg tctcgtgatg actcgttgat
      601 gatttttact tttggtacat tggtttagca gaggacagtt cacacattct tttggattgg
      661 atttgtattc tcggtaccct tctcgagtaa ccgttgaaaa agcgagcatt tgatcttgcg
      721 gacaaatata acaatcaaaa tactcgtcat agagaaactc attttttgat aatagcccct
      781 ttttcccttt gggccttgaa taaggcaaac aaggtcgaag gttcaaatga gtcaaaaagt
      841 gaacaaactt aggtgtctta tacccagcat cagcgacaac acaattgatt tcaggaaaaa
      901 gtctcttcga ttgtttgacg agctgaaagg cgacttgact gtcatgaata tttccaggag
      961 tgatgattgt gctaagaata aatccattgt cgtcgcatga agtatgggca gaataagcaa
     1021 attgtttttc tcgttcgcct ttgacgtagt agccgctttc cggatcagct ttacttattt
     1081 ttctttcttt cagcttcact tccttttcta agtccaaagt aaaagggcgt tttccagcct
     1141 ctattcgttg acgattgatc tcatcttcta attcatcctg ataggctttc gcttcatcat
     1201 gggtcatttc tttcgtaaac ttatgtttat tggcgttagc tttgatgtgg gtagaatcca
     1261 gatacaaatt atcctctgtt acaaaacctg ctttaactgc ttgttctaaa atataggcaa
     1321 aaatatcctc aaacaccgtc gtttctctaa atcggcgaac ataattcttt ccaaaagttg
     1381 agaaatgggg gattttctcc tcgaaggagt agcctaaaaa ccagcggtaa gcgacattgg
     1441 tatcgacttc cttaatggtt tgtcgcatgg aacggatacc gaataaatac tgaataaaca
     1501 ccaatttgat cagaatcact ggatcgatac ttgggcgacc taaatcggag taagtggctt
     1561 cgacgattgg ataaataaat tcaaaatcaa gcgcggcatc aatctttcga acgagatgat
     1621 cttgaggaac tagatcatca atcgtaagca tcgcaaattg atttctacca ttcgtattgt
     1681 ttttagacat catagctatc actccttgca ttcgttttca taaatctatt ataaaagaaa
     1741 agactgtaga catcatccat aaaatggact ttgtctacag tct
//
LOCUS       DAZUMM010000167         1752 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000167 DAZUMM010000000
VERSION     DAZUMM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.181"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..687
                     /locus_tag="ACG8E6_002695"
     CDS             85..687
                     /locus_tag="ACG8E6_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017865320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5063043.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQNKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRQRCGISVADRPLD"
     gene            760..1149
                     /locus_tag="ACG8E6_002696"
     CDS             760..1149
                     /locus_tag="ACG8E6_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063044.1"
                     /translation="MKKLFANEKIDLLYSIILVLIWIIFLLISKLLHFHSEWVISFIG
                     VFVMGCINLPTILRRKKQYKKIDELRKVLNLSIKEVREIADIGRYDLSDWNWDKAHIS
                     PKKLYLLEDTLEKMYVKQFGKEFEMRK"
     gene            1322..1648
                     /locus_tag="ACG8E6_002697"
     CDS             1322..1648
                     /locus_tag="ACG8E6_002697"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063045.1"
                     /translation="MALSQVCMVSFLQRFLGSTYRNYFIVVFWLLLSLIAFIVFVFNF
                     ESLWQDIAYWIVPSKDLYINKPEIIRDLIKVVPMIILGIVVFVIGIRQGLKNYYGDYS
                     GDTKQQ"
BASE COUNT          617 a          223 c          364 g          548 t
ORIGIN      
        1 tttctgataa gcaatttgaa attgaaaaat tagatatatc tgattggtta ttgcaaccga
       61 gagaatttta gaggttggtt gaaaatggcg aaaattggtt atgcacgtgt cagtagcaaa
      121 gaacagaact tagatcggca attgcaagcg ttacagggcg tttctaaggt cttttcagac
      181 aaattaagcg gtcaatcggt cgaacgtcca caattacaag ctatgcttaa ctatattcgt
      241 gaaggggata ttgttgttgt tactgaatta gaccgcttag gacgaaacaa caaagaatta
      301 acagaactga tgaatcaaat tcaaaacaaa ggcgcaaccc tggaggttct aaatttaccg
      361 tcaatgaatg ggattgaaga tgaaaattta agacggctga ttaataattt agtgattgaa
      421 ttgtataagt accaagcgga atctgaacgc aaacgaatta aagaacgcca agcccaagga
      481 attgaaattg ctaagaaaaa aggaaaattc aaagggcgac aacctaaatt caaagaaaat
      541 gatccacgtc tacaacacgc ttttgatttg tttttgaacg gtttatctga taaagaagtt
      601 gaagaacaaa ctggaatcaa tcggcgaacg tttagaagat accgacagcg gtgtggcatt
      661 tcagtagctg atagaccttt agattaatat gaacgtggtg aagataacat aaaagagaaa
      721 acaagaaagt aatagtaata gaaatgaggg gtagacatta tgaagaaatt gtttgcaaat
      781 gagaagatag accttttata ttcaattatt ttagttttaa tttggattat tttcctactt
      841 atcagtaagt tgcttcattt tcactctgaa tgggttattt cttttatcgg agtgtttgtt
      901 atgggatgca tcaacttacc aacgatttta cgaaggaaaa aacagtataa aaaaatagat
      961 gaactaagaa aagtgttaaa cctatccatt aaagaagtaa gggaaattgc cgacattggt
     1021 agatatgatt taagtgattg gaactgggat aaagcgcata tttctccaaa aaaattatac
     1081 ttactggagg acaccctaga aaaaatgtat gttaaacagt ttggaaaaga atttgaaatg
     1141 agaaagtagt tttttaagga aatattccaa gtggaggtat tcaaagctat tcaagatcga
     1201 aagattcttt tggttatatt tttgttggtt ctgattgtca gtgtggtgtt aatcattggt
     1261 agttttaaaa taaggaaaca aagtagggga acggctttat ttttgctttt attgggaata
     1321 gttggcatta tcgcaagtct gtatggtttc ttttttacaa cgttttttgg gatcaactta
     1381 taggaactat tttatcgttg tgttttggtt attgctgtct ttaattgcgt ttattgtttt
     1441 tgtatttaat tttgagagtt tatggcaaga tattgcttac tggattgttc caagcaaaga
     1501 cctttatata aacaaacctg aaatcataag agatttaatc aaggttgttc caatgattat
     1561 tttaggaatc gtagtttttg tcattggtat tagacaagga ctgaaaaatt actatggcga
     1621 ttattcaggt gacacgaagc aacaataact aaagctatct aaaattagga aaaaaggaga
     1681 gaatgaatga gtacagttat tttagctgaa aaaccaagcc aggcgttagc ctatgcccag
     1741 gcatttaatc ag
//
LOCUS       DAZUMM010000168         1742 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000168 DAZUMM010000000
VERSION     DAZUMM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.182"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACG8E6_002698"
     CDS             <1..1412
                     /locus_tag="ACG8E6_002698"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HFS5063046.1"
                     /translation="IFTPKRVVKKMVDKLEEENPGIFSDRTKRFADLYAKSGLYITEI
                     VKKLNEGLKGQIPNQQERIKWILENQVYACAPSNIICNIVKNFVYSHTINVSTKNLIE
                     VDTMKLASEEKLSTEIYELFGDDRLKFDVIIGNPPYQDETIGENKTYAPPIYHKFLEE
                     SYKLADKVEMIHPARFLFDAGSTPKAWNRKMLADENLKVIDYVDDSSKVFPNTDIKGG
                     IAITYRDAQKRIGPIGTFTKYPELNTILRKVKMDNFNSIDKIIYSADSYKFTQALHDD
                     YPDAEARLSKGHAFDVRSNVFDSIGHIFKNEKPSDNSEYIRIIGRQNHKRIEKWVKRD
                     YIGEHPNLDKYSVLLPGSNGNGDFGETFASPIIKEAAVAHTQTFISIGHFDNIIEAQN
                     LLKYVKTKFARAMLGILKATQANSRPKWKWVPMQNFTNESDIDWSESISEIDKQLYKK
                     YNLNQNEIQFIEDKVKPME"
BASE COUNT          651 a          235 c          316 g          540 t
ORIGIN      
        1 agatttttac gcccaaaaga gtagtaaaaa aaatggtcga taaattagaa gaagaaaatc
       61 ctgggatttt ttctgatagg acgaaaaggt tcgccgactt gtatgcgaaa agtggtttat
      121 atattacgga gatagtaaag aaattaaatg aaggtttgaa aggacaaatt ccaaatcaac
      181 aggaacgtat taaatggata cttgaaaatc aagtatatgc gtgtgcacct agtaatatta
      241 tttgtaacat tgttaaaaac tttgtctata gtcatacgat aaatgtgtca accaaaaatc
      301 ttatagaagt tgatacaatg aagttagcaa gtgaagagaa gttaagtact gaaatctatg
      361 agttatttgg agatgataga ttgaaatttg atgtaattat tggtaatcct ccttaccaag
      421 atgaaacgat aggagaaaat aaaacgtatg ctccaccaat ttaccacaag tttttggagg
      481 agtcttataa actagctgat aaagttgaaa tgattcatcc tgctcgattt ttattcgatg
      541 ctggtagtac accaaaagca tggaatagaa aaatgttagc cgatgaaaat ttgaaggtta
      601 ttgactatgt ggatgatagc tcaaaagtat ttcctaacac cgatattaaa gggggtatag
      661 cgataaccta ccgtgatgct caaaagagaa taggtccgat cgggaccttt acaaagtatc
      721 cagaattaaa tactatcctt cgtaaagtga agatggataa ttttaattca attgataaaa
      781 ttatttattc agcagatagc tataagttta cacaagcatt acacgacgat tatcctgatg
      841 cagaggcacg attaagcaaa ggtcatgcat ttgatgttcg ctctaatgta tttgatagca
      901 ttggacatat atttaaaaat gaaaagccct cagataatag tgaatatatt aggattatag
      961 gtagacaaaa tcataaaaga atagaaaaat gggtgaaacg tgattatatt ggtgaacacc
     1021 caaatttaga taaatatagt gttcttctac caggatcaaa tggaaatggt gactttggag
     1081 aaacatttgc ttctcctata ataaaagaag ctgctgttgc acacacacaa acgtttatta
     1141 gtattggtca ttttgacaat ataatagaag ctcaaaacct gttaaaatat gtaaaaacaa
     1201 agtttgcgcg agcaatgtta ggaatattaa aagcaactca agctaatagt cgaccaaaat
     1261 ggaagtgggt tccaatgcaa aactttacta atgaatcaga tattgattgg tcggaatcaa
     1321 tatctgaaat tgataaacaa ctatataaaa aatataattt gaaccagaat gaaattcagt
     1381 ttattgaaga taaagtaaaa ccaatggaat aaaatataag caagacattg ttgtattcaa
     1441 ccagcatcta tggttcaaat tggtgcaagt aatgtcaatt tcaatccttt ttcatacatt
     1501 ttcatccata taataattcc caaaacggtg atataaatgg attgcgatat attcaataga
     1561 cttagaatta atctgtatat ttctcaaaac gttaatcgtt gttatatcaa ggatttcacg
     1621 gcatcatccg tttggatggt gtcttttttg ggttgaattc cttgaaaatt gggtctattt
     1681 tacagaccct tttctatata aactgttttc gatacttgga aaaatctgtt gtttttaaat
     1741 tt
//
LOCUS       DAZUMM010000169         1712 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000169 DAZUMM010000000
VERSION     DAZUMM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.183"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..631
                     /locus_tag="ACG8E6_002699"
     CDS             17..631
                     /locus_tag="ACG8E6_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5063047.1"
                     /translation="MRVAYIRVSSIDQNEQRQVEEMKKFGAERIFIEKQSGATITHRP
                     VFQEVLDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSTVNHE"
     gene            complement(655..1005)
                     /locus_tag="ACG8E6_002700"
     CDS             complement(655..1005)
                     /locus_tag="ACG8E6_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5063048.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(999..1262)
                     /locus_tag="ACG8E6_002701"
     CDS             complement(999..1262)
                     /locus_tag="ACG8E6_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFS5063049.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            1416..1565
                     /locus_tag="ACG8E6_002702"
     CDS             1416..1565
                     /locus_tag="ACG8E6_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063050.1"
                     /translation="MFKKTKSLESGKKKIDLKIISSGYFKDFIVIDTKWNKKDIENIL
                     NSRNN"
BASE COUNT          574 a          306 c          261 g          571 t
ORIGIN      
        1 gaaagtaggt aaaaatttga gagttgcata catacgtgtc tcatcgatcg atcaaaatga
       61 acaacgccaa gttgaagaaa tgaaaaaatt cggtgcagag agaattttca tcgagaaaca
      121 atctggcgct acaattactc atcgacctgt tttccaagaa gtattggatt acgtccgaga
      181 tcaagatatt tttattgtgg aagctattga tcgacttgga agaaattacg acgagatcat
      241 cgcttcagtc aattacttga agaagaaaaa tgtgcagctg atcattacaa gtctaccgat
      301 catggccgaa gcaattggca atcctctcct ggataaattc attaaagatt tgatcattca
      361 aattttggcc atgatcgctg aacaagaacg taccgaatct aaacgacgtc aggcacaagg
      421 cattaagatt gcgaaagcga acggtgttta taaaggacgt cctaagctat acagtgccaa
      481 tgcaaaagat ccccaacgtc gtcttgttta taaaaacatc gttgaggatc taaaaaaagg
      541 tgttgcgatc gctaagattg caaaagacta caatgttacg agacaaaccg tatatagaat
      601 aaaaaaagac agcacggtca atcacgaata gttgatcatg ctgctgtttc tattttattt
      661 gcttactttt tgaaaaactg ttctaacttt catcaataac tcctcaacta aactatcatg
      721 cgctttttcc aaaaaaatac actctctggc ttcataatca attgtcgtta gttgatctaa
      781 taatactttt cctgttgtct tcatttcata ttctgagttt atatttacat atagcggata
      841 gtttctagtt gtactagaaa ttggagcaac tgtaacaacg ttactataat ccgcaataat
      901 atcatgactt aaaacaatat aaggcctttt acctgtctgt tcgtgtccag atcgaggtgc
      961 tgtattcaat aatagaatat cgccttgtct aggttttctc accatttttc attacctact
     1021 gcttcaccta aattttgaat cgtcacatga tttctttcat cagtgtaatt ttcaaataac
     1081 tgttcaattg ttaattcatt ttctatatta gccgcttcga taattagccg cttctgaccg
     1141 tttacttctt cttgtattat cttcactttg tcttctgtat gaattccaag atattccata
     1201 atatactttg gcaatctaag gccattacta tttccccatt tttttatagg taacatttcc
     1261 atagtagaca cctctttctg tatatacata gtataacata cataagaaaa aacgtatata
     1321 cttaattatc cttttctatt aaattagatt aacattccat gaatccataa actatgttaa
     1381 catttatatg aaaatagtcg aaaggaaatc ataatatgtt taagaaaaca aaatctttag
     1441 aatcaggtaa gaaaaaaatt gatctaaaaa taatctcttc tggatacttt aaagatttca
     1501 tagttattga tacaaaatgg aataaaaagg atatagaaaa tattctaaat agtaggaaca
     1561 attaagtgct atcacttttg tttcttcaaa tgaaaagtga cgtcactttt tgagtgctgg
     1621 tacatcaaat cctgcttttt cttgcgcgat caatagatct aacgcaatac acataatctt
     1681 agattcactt tttccgtact tctgctttag tt
//
LOCUS       DAZUMM010000170         1668 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000170 DAZUMM010000000
VERSION     DAZUMM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.184"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            53..1321
                     /locus_tag="ACG8E6_002703"
     CDS             53..1321
                     /locus_tag="ACG8E6_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS5063051.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
BASE COUNT          553 a          318 c          320 g          477 t
ORIGIN      
        1 tttagttaca ctgttattta ggaaaagttt cataattcaa aggagaaaaa agatgaaagc
       61 aaagaattta ttatttagcg ttattctttt aggaaatcta gcagttgcca acacaacagt
      121 gctagctgaa gaatcgacag aaactgtcgc aactcagaca tcgagttctt cagaaacagg
      181 aattgaaaac gatacagaga gtactttgca gtcagctact gtctcagaaa atacaatttc
      241 aaccgaagaa ccaacaactg aaacaacaga acaaacacaa accgaagaca gtacatcagc
      301 agaagctcag aacaatccag agaatgcaga agaaatcacg aaccaattga cagatgggca
      361 aacattgaca catacccatc aagcagattt gtctgaattc gaaaactata ctggctatgc
      421 tttttatgct cgctcatctg ctaattctca acaagcgttc attgatagta ttgcctcaac
      481 tgcacaaagt ttggcaagtg ccaatgactt atatgcatca gtcatgattg ctcaagccat
      541 cgttgaaagt ggctggggga acagtacttt ggcttctgcc cctaattaca atctatttgg
      601 gattaaagga agctataatg gccaatctgt tacgatgcct acttcggaat atgtgaatgg
      661 gcaatggatt acggtgaatg ccgcttttag aaaatatcct tcgtacaaag aatcattgca
      721 agataacgta acggtattga agacgacttc ttttcagcca ggcgtttact actattccgg
      781 tgcatggaaa agcaatacga actcttacaa agatgctacg gcttggctaa caggtcgtta
      841 tgccactgcg cctaattatg gttctacatt gaacaatgta atcgaaacct ataatctgac
      901 acagtatgat acagcaccaa cttcaacttc tccgatgtat cgtctgtata accgtcatac
      961 aggagaacat ttatatacat tgaatgccgg agaaaaaaat tatctgccaa ctgtaggctg
     1021 ggaatatgaa gggattgctt ggcaagcacc aaacagcgga caacctgtct accgtttata
     1081 caatccttac agtggagacc atcattacac aatggcacaa tctgagatca actctttaac
     1141 aaaaatcggt tggcgttatg aaggtctaag tttttattct ggtggttcca agccaattta
     1201 tcgactattt aatccaaatg aaaaaactgg tacccatcac tataccttga gtgctaagga
     1261 aagagattgg ctaacaccga tgggctggag atatgaagga ataggatttt atggttatta
     1321 aaaagaggta gtgaaaaagc tgccctgcat caagtagtaa gaacagccaa acaaaaacag
     1381 attttcatgt tttttgtttg gttgaactta ttaccgcaga tgcaagtttt tgaacaccgt
     1441 ttactcaaaa aaggttgtga caacatcttg tcataacttt tttattactg tttaagcata
     1501 ctgatccaat ttctaatatt cgacgggatt cttgcaaaag gcgatttcgg aaatttttgg
     1561 cggtgtaaaa ttttctactg cagaagagct atcttctata gtttgataag aatcaataat
     1621 cttggtttta gcgttgtgct ttctttatca ttttttccgt tcattcta
//
LOCUS       DAZUMM010000171         1665 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000171 DAZUMM010000000
VERSION     DAZUMM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.185"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..506
                     /locus_tag="ACG8E6_002704"
     CDS             132..506
                     /locus_tag="ACG8E6_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS5063052.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(620..1249)
                     /locus_tag="ACG8E6_002705"
     CDS             complement(620..1249)
                     /locus_tag="ACG8E6_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5063053.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1383..1455)
                     /locus_tag="ACG8E6_002706"
     tRNA            complement(1383..1455)
                     /locus_tag="ACG8E6_002706"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          558 t
ORIGIN      
        1 tctacactct gagatgtaag aaatatcata agatatttct tacatctttt tgttttgagc
       61 aggactttag ataattttat taatatatgc tagcatgctt ttgtaagaaa aagaaagagg
      121 gcgtattagt tatgtatctt aaaattacgg aacaagcaca agaaagatta caaaaatata
      181 ttgatgaagg cgcaacagtt atattggatt tagatgatgg cgttggggaa tattctaaaa
      241 tgggtgtttg ctctttagat actagttttc gtttacttct tcttgataaa gaacagagaa
      301 aaaatgatta taaactccaa ttagatagtg atattgggga tgtttatata aaagattatt
      361 caaaaatgta catggatgaa catatgaccc tttctttaga tccacgtcta ggtgtattta
      421 ctcttgaaag cccaagtggt tctttagaca gtcatgttca attagtagat ctacgaccag
      481 aaaaattctc tggaataaaa caataacttt ctctctattg tccataaaac tagtctcaat
      541 caagctgtat tgaaaaaaca agacaacatt cttaccatgg taaataaaaa gacgacacat
      601 tttgtcgtct ttttttaaat taactatttt cgataaaaga tcgcatagct tgtgccatta
      661 tttctggctt ttctctattc aattcatgtt ttccgttttt aataaagagt agccggccat
      721 tcgttgaaag tttatataaa cgttttgctg ctggtttatt cgcatgatct ttggtaccgc
      781 ataaaatcaa tttaggaagt cgtgatgtac gaagctgttt tttgcagtct aatgtttcaa
      841 gtgaacgaag tagggtaagg gtttgttttt tagatagacc taatttttca aatatcttag
      901 aaggtacgca gtaaaaataa atattctgga aagtaagcaa taagcgatta ggagactcaa
      961 attgtggagc gactaaaaca acctttttta aatatggaga aggagttgca agatagttta
     1021 aagcaataac agcgcctaaa gataatccac ataaaataaa aggttccttt atctgattca
     1081 attgtacttc gatgtatttg gttaagtcgt ctaaagtagc agtgtctgtg ctgtttaaat
     1141 gattgaacaa tgaaagactt ttgcaagaat aactattggg tagataagag attacatctt
     1201 tccatgattg ttcgtcttgg cctagaccat gcaaaaaaac aattttcata ttgctctcct
     1261 ttttatagtt atcatataga ctttctttgt actattaaaa tataaacatt catttcttac
     1321 gtcataaaat aaaaaaacag cgcagacaca ttgttaaacc aacatgttgc actgtttaag
     1381 gaagccactt gccgggcttg aaccggcgac ctcttcctta ccatggaagc gctctaccag
     1441 ctgagctaaa gcggcattat cattaataca aataatagga taacgaatat taatgtattt
     1501 gtcaacagaa ttttttcgaa aaaatgtgtt tctctaatag gaaaacagat tttagaaaaa
     1561 ataaaaataa caaaaaatgc gaacggtttc tttgataata taataaaaaa attcaaaaaa
     1621 agtattgcgt tatttagaag aaattgatat tatattcatg tactt
//
LOCUS       DAZUMM010000172         1647 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000172 DAZUMM010000000
VERSION     DAZUMM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.186"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACG8E6_002707"
     tRNA            complement(20..91)
                     /locus_tag="ACG8E6_002707"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACG8E6_002708"
     tRNA            complement(113..200)
                     /locus_tag="ACG8E6_002708"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACG8E6_002709"
     tRNA            complement(225..298)
                     /locus_tag="ACG8E6_002709"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACG8E6_002710"
     tRNA            complement(312..382)
                     /locus_tag="ACG8E6_002710"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACG8E6_002711"
     tRNA            complement(384..459)
                     /locus_tag="ACG8E6_002711"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="ACG8E6_002712"
     tRNA            complement(478..551)
                     /locus_tag="ACG8E6_002712"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
     gene            complement(564..653)
                     /locus_tag="ACG8E6_002713"
     tRNA            complement(564..653)
                     /locus_tag="ACG8E6_002713"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ser,seq:tga)
     gene            complement(677..750)
                     /locus_tag="ACG8E6_002714"
     tRNA            complement(677..750)
                     /locus_tag="ACG8E6_002714"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ile,seq:cat)
     gene            complement(764..837)
                     /locus_tag="ACG8E6_002715"
     tRNA            complement(764..837)
                     /locus_tag="ACG8E6_002715"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Met,seq:cat)
     gene            complement(865..937)
                     /locus_tag="ACG8E6_002716"
     tRNA            complement(865..937)
                     /locus_tag="ACG8E6_002716"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ala,seq:tgc)
     gene            complement(957..1030)
                     /locus_tag="ACG8E6_002717"
     tRNA            complement(957..1030)
                     /locus_tag="ACG8E6_002717"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Pro,seq:tgg)
     gene            complement(1040..1113)
                     /locus_tag="ACG8E6_002718"
     tRNA            complement(1040..1113)
                     /locus_tag="ACG8E6_002718"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1077..1079),aa:Arg,seq:acg)
     gene            complement(1124..1209)
                     /locus_tag="ACG8E6_002719"
     tRNA            complement(1124..1209)
                     /locus_tag="ACG8E6_002719"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1173..1175),aa:Leu,seq:taa)
     gene            complement(1226..1297)
                     /locus_tag="ACG8E6_002720"
     tRNA            complement(1226..1297)
                     /locus_tag="ACG8E6_002720"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1263..1265),aa:Gly,seq:gcc)
     gene            complement(1309..1381)
                     /locus_tag="ACG8E6_002721"
     tRNA            complement(1309..1381)
                     /locus_tag="ACG8E6_002721"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1346..1348),aa:Thr,seq:tgt)
     gene            complement(1399..1480)
                     /locus_tag="ACG8E6_002722"
     tRNA            complement(1399..1480)
                     /locus_tag="ACG8E6_002722"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1444..1446),aa:Leu,seq:tag)
     gene            complement(1488..1560)
                     /locus_tag="ACG8E6_002723"
     tRNA            complement(1488..1560)
                     /locus_tag="ACG8E6_002723"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1525..1527),aa:Lys,seq:ttt)
     gene            complement(1564..1636)
                     /locus_tag="ACG8E6_002724"
     tRNA            complement(1564..1636)
                     /locus_tag="ACG8E6_002724"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1601..1603),aa:Val,seq:tac)
BASE COUNT          438 a          460 c          391 g          358 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      661 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      721 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      781 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      841 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      901 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      961 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     1021 ctacttcccg tttaattaac gcgcccaaga ggagtcgaac ccctaacctt ttgatccgta
     1081 gtcaaacact ctatccagtt gagctatggg cgcatatttg aaatgccgag gaccggaatc
     1141 gaaccggtac ggtgatcact caccgcagga ttttaagtcc tgtgcgtctg ccagttccgc
     1201 caccccggcg cgattgcttt ggcaaagcgg aaaacggggt tcgaacccgc gacccccacc
     1261 ttggcaaggt ggtgctctac cactgagcta tttccgcgtg tttaaagatg ccggctaaag
     1321 gacttgaacc ctcgaccctc tgattacaaa tcagatgctc taccaactga gctaagccgg
     1381 catgtaaaac ttaaattatg cgggtgaagg gacttgaacc cccacgccgt aaggcgctag
     1441 atcctaaatc tagtgcgtct gccaattccg ccacacccgc aaaaatatga gccgtacagg
     1501 gctcgaacct gtgaccctct gattaaaagt cagatgctct accaactgag ctaacggctc
     1561 atatggaggt taacgggatc gaaccgctga ccctctgctt gtaaggcaga tgctctccca
     1621 gctgagctaa acctccatgg ataactt
//
LOCUS       DAZUMM010000173         1642 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000173 DAZUMM010000000
VERSION     DAZUMM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.187"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..706
                     /locus_tag="ACG8E6_002725"
     CDS             <1..706
                     /locus_tag="ACG8E6_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5063054.1"
                     /translation="ELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFS
                     FEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKK
                     FVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIE
                     EFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSS
                     LTSDLPKISLRNWLEE"
     gene            complement(843..1259)
                     /locus_tag="ACG8E6_002726"
     CDS             complement(843..1259)
                     /locus_tag="ACG8E6_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063055.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1262..1546)
                     /locus_tag="ACG8E6_002727"
     CDS             complement(1262..1546)
                     /locus_tag="ACG8E6_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063056.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
BASE COUNT          518 a          246 c          283 g          595 t
ORIGIN      
        1 ggaattaaca gtcaaatatc aaaatgaatt gaatttagta ccgctgaaaa atttcaatgc
       61 taagcagatg gatttatttt ttgcattatg cgctcgtatg aaagatcgtg gtttgaaaaa
      121 agttagattt tctttcgagg aattaaaaga gttaagtgat tataagatga ctgctacaaa
      181 agcctttgtc ggtgatttag aaaagctcta taaagatatg cttaatctta cttaccgtac
      241 tgaagatgag gattctattc gctactttgt tttgtttact ggttttgaaa tcaataaaac
      301 aaaaaagttt gttgaagtca gtgttaatcc ggacctagaa tatattatta atggtttaac
      361 agctgaattt agtagatttg aattatctgc tttcacgtcg ttgcgttcta cgtatgcaaa
      421 aactctattc cgactgctta tgcagtaccg ttctactggt tattatgtag ttcgtattga
      481 agagtttagg agcttaatgg atatcccaga atactatcaa atgggtaaca ttgatcaaaa
      541 agtattaaat cctgcattac gagaactaaa aaaatacttc aatgatctta aagtcacaaa
      601 aataaaagca aaaaagggca ataaaatcgc caaattagaa tttacttttt caagcctaac
      661 gtcagatttg ccaaagattt ctctgcgtaa ctggttagaa gaataaataa aaaagaactc
      721 gcccccctta cattaagcaa gtagacaagt cccttacaac cgactaagat tgctagtaca
      781 ttctagcata ttgtatgggg cttctaaata aaaaacagat aggaataact cctatctgtt
      841 ttttatcgtt ttagaaaatt tgttgggtcg aaatttctag aagtgactgt ttgactggtt
      901 tctttttgat gattctttgg tcgatttcga tttgatggat ttagtttagc ttcgattttt
      961 tctttcactt cttgatatac tttagaattt gtgccaataa gaacgcctgt atcttttaga
     1021 tataaatcag caaattctgc gatgatttca gtcatgatgg aaatttcttt tcccatagca
     1081 tttagttttg cctgattgct ggtagtggtc gtgttggtat cgggttcata actcatacat
     1141 aaaaagcgaa tggcttccga gtagttattg atatctggat ttttttcgat gatggaatct
     1201 aaaacgtcaa catgactagg gtacaaagta acatgttttt ggacagaagt ttttctcatg
     1261 gttatatcat tcctccgata agcatttcaa tgatttgatt atttgtgtag acatactcgc
     1321 ttaatatttg agtattttct gtttggatag caataaattt atcgagcaat aaatcgaatt
     1381 ggtctttttt gtaagtcgta agttgacggt caaaatcact ttcgattaaa acttttagat
     1441 atttatttcg actccatttt tctcctgttt tattcgtgag ttcttggcaa cgtttgtcta
     1501 ttgttgcaac aaactttgga tcaatattcc gaactaaaat gtccataaaa aatctccttt
     1561 tgcaattatg tataatttat gtataccata caaatactat atttgtatag ctgttttttg
     1621 ccaaaatgct atacataagc ta
//
LOCUS       DAZUMM010000174         1604 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000174 DAZUMM010000000
VERSION     DAZUMM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.188"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..582
                     /locus_tag="ACG8E6_002728"
     CDS             <1..582
                     /locus_tag="ACG8E6_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS5063057.1"
                     /translation="LDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYD
                     LAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIENANLHVAGWHI
                     ANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSFNMRNFPNKKV
                     YVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            771..844
                     /locus_tag="ACG8E6_002729"
     tRNA            771..844
                     /locus_tag="ACG8E6_002729"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:805..807,aa:Ile,seq:cat)
BASE COUNT          535 a          288 c          293 g          488 t
ORIGIN      
        1 ttggacactc cttataacca accgggaatc aaatcgcatt tatgggtaac acaaaacatc
       61 tggggcgatc atacagatcc ttacggttat ctttctgaaa tgggcgtaag taaagaaaaa
      121 ttagcatatg atttagctca tggatttacc gatgaaaatc caacaacttc tgaaaacaag
      181 cctgtcattg atccaacacg agctggtgca gctaatccta cactgacaga tggaacgaat
      241 tacgcccaca ttgatcagtt tggagaaatc gaaaatgcaa atttgcatgt agctggatgg
      301 cacattgcta actataaata cgagtatatc ttcattatgg attacaatac tgggaaagaa
      361 ttagctcgag taagagctga tggaatttat agatcagatg taaatcaagc ttataatact
      421 tctggaaatg ttggctatca tgtatctttt aacatgcgta acttccctaa taagaaagtc
      481 tatgtcatga tgcgggcaac gaatgatcca gagggaaaca ctaaaggcgg tgcgcaagat
      541 ttccatgaca aacgttggta tttaaatatt cctaaacgat aaaaatagct cctcgttgag
      601 gagcattaca taactatatt gacaactata aaaatcattc gataaaatag tgatgttatc
      661 gcatatcttc actatcaccc ataatagtca cactccaagc tatgcgataa caggtttgtt
      721 gccacacatt ctactggttg attgtttatg gctttatgtg gcaacaacca gtacccttag
      781 ctcagttggt cagagcagac ggctcataac cgtccggtcg taggttcgag tcctacaggg
      841 tacattaacg tagccatttg aatcgttctg tgttagaatt ttttgaagag tattatacaa
      901 gctaaagctt ttcttcattg ccactcaaat gagtggcttt tttatgtatc cttttatgga
      961 ttaatgaaag gatgtttcac atagttatat ttctgtatat ttgaaaagtt ttactttgat
     1021 ttttaaatag aaagacattt gggttaaatt gtgagatgat aataaagaag agtttaaagc
     1081 gcaccccaag ccacttcccc ataagtgtgt tacgctttaa actcttttat atttgaagct
     1141 attaaaaggc ataccatatt tttgaaaaaa agtgagaaaa aaggcttaca attggagtgg
     1201 tagttaatta gtgacttatt tttgatttta tagcactgat actataaaat atagatatca
     1261 tcatattaca caatcttaat actaacttaa aaaatatctc ctttcacaag tatggtgata
     1321 aaattcgttc cgggctacct ttttaggtag cctactttaa tctttatacc tttctggatc
     1381 aacgaaagta tactttatat agtcataacg ccgatgatcg ctacgtgcgt ccggcacgtc
     1441 agtcatgaat cggctacact agactagaca gaaaaaataa ggtgtgtaga ataataaaaa
     1501 acacactgga ggatttttca tgtcaagacg tcaacgaaga acctattcaa aagaattcaa
     1561 acaacaaatc gtcgatctct atctcgctgg taagcctcgc gcag
//
LOCUS       DAZUMM010000175         1603 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000175 DAZUMM010000000
VERSION     DAZUMM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.189"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..667
                     /locus_tag="ACG8E6_002730"
     CDS             71..667
                     /locus_tag="ACG8E6_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS5063058.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(725..1207)
                     /locus_tag="ACG8E6_002731"
     CDS             complement(725..1207)
                     /locus_tag="ACG8E6_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063059.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1313..1510)
                     /locus_tag="ACG8E6_002732"
     CDS             complement(1313..1510)
                     /locus_tag="ACG8E6_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063060.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          542 a          331 c          251 g          479 t
ORIGIN      
        1 taaggtgcta tacttcattt cgtaatcaat taaaaaaatg caaaacgcaa atcaaaggag
       61 aaattttatt atgacaactt ttactgacac tttgaaaaac cgccgttcaa tctatcatct
      121 aggacgtaat gtttcacttt ctaatgaaga attaacaact ttgatcaaag aagcaattaa
      181 agaaagccca actgctttca atgctcaatc aacacgcgca gttatccttt tcggagatgc
      241 acatgaaaaa ctttgggaaa tcactgaaga agcacttcgc ccattgactc cagcagaagc
      301 tttcccaaat acacaaaaca aattagctgg tttcaaaaat ggttatggta cagtattatt
      361 cttcaaagat actgacgtgg taaaaggtct acaagaacaa tttgaattat atgcagacaa
      421 cttcccagat tggtcagaac aatcaaacgg aatcgcaaca gctaatactt gggtagcatt
      481 agttgataaa ggattgggcg caaacttgca acattacaac ccagttattg acgaagcagt
      541 tgcaaaagaa tggaatatcc cttcaaactg gaaactacgt tcgcaattag tattcggttc
      601 accagaaaca ccggctgggg aaaaagaata catgaacgat gccgatcgct tccgcgtatt
      661 tggataattt tatttgaaat aaaaaacaga ggttgtgaca aacatcttgt cgcaacctct
      721 gtttttattc tggatcaatc ataatatcaa tctctgcctc atcgtttaag cgttgggaca
      781 aaaatggaac taattcttct aatgatgcag aaaatttcag catcggtcct cgccatccgt
      841 ttcggaaaaa attcattttt acgattttag aaccttttgg ctgatataat acaattttat
      901 tgatctcaga aaaaggaaca ccttttttgt caaagctatt caaaacaagt ccttcttctg
      961 tgattcctct actgtccaac aaaaaactaa taaaaatcat tgctgacaag atcccttttg
     1021 ccaattcatc tgctgtagga tgactgatga gtgtatacca aatgactagt gacactaaga
     1081 agggtatcac caagtagacc catcgatttc gcacagtccg ataaataatt tcttttcgaa
     1141 ccagccaaag aaataaaaac atcacaacaa cgaataaaaa caatagaacc gtaaagaatg
     1201 ttaccatgac aactcgcctc gctttttctt ctccagaaaa taggaaaaga atactgattt
     1261 accttctcaa agaaattgga cttgcagcac gccgcacgtc caattgtttt ctttattcaa
     1321 atgtatcata ttttgcaaca accatgcttg ctttttcatc taataattct tggtcctctg
     1381 cagcacattg ggcaactttg accacggcgc agtttgtcat ttttgataca acttcaccaa
     1441 tgaccgtttt tgtaaaacca attggtttgc atgcatattg ttctcctaaa ttaatcattt
     1501 cttccgtcat caaaatcctc ctaaactgct ggaacttttc tccacattac cactgctatt
     1561 tctttggaga tttaactcat tgatttttat ttacagttaa aaa
//
LOCUS       DAZUMM010000176         1562 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000176 DAZUMM010000000
VERSION     DAZUMM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.190"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..105
                     /gene="ermCL"
                     /locus_tag="ACG8E6_002733"
     CDS             46..105
                     /gene="ermCL"
                     /locus_tag="ACG8E6_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HFS5063061.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            153..887
                     /locus_tag="ACG8E6_002734"
     CDS             153..887
                     /locus_tag="ACG8E6_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HFS5063062.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          584 a          247 c          211 g          520 t
ORIGIN      
        1 cttgtgctat aattattcta atttcataaa aaggagaatt aatatatggg catttttagt
       61 atttttgtaa tcaacacagt tcattatcaa ccaaacaaaa aataagtggt tataatggat
      121 tgttaatata aacctcatag gagtgggtta taatgaacaa aaaaaatata aaagatagtc
      181 aaaactttat tacttcaaag catcatataa atgaaatttt gagaaatgta catttaaata
      241 caaatgataa tattattgag attggttcag ggaaaggtca tttcacgttt gaattagcta
      301 aaaggtgtaa ttatgtaacc gccattgaaa tagatcctaa attatgtagg ataactaaaa
      361 acaaacttat tgaatatgag aacttccagg ttatcaataa agatatttta caatttaagt
      421 ttcctaaaaa taagtcatat aagatatatg gaaatatacc ctacaatata agtacagata
      481 taattcgaaa aattgttttt gaaagcacag ctacagaaag ttatttaata gtggaatatg
      541 gatttgctaa aaggttgcta aatacaaatc gttcactagc actattttta atgacagaag
      601 ttgatatatc catattaagt aaaatcccta gagaatactt tcatccaaaa cctagagtta
      661 atagctcgtt aattgtatta aaaagacacc cttcaaaaat atcactcaaa gatagaaaac
      721 aatatgaaaa ttttgttatg aaatgggtta acaaagaata cataaaacta ttttccaaaa
      781 accaatttta tcaagcctta aaatatgcaa gaattgacga tttaaacaat attagctttg
      841 aacaattctt gtctcttttc aatagctata aattatttaa tagataagtt aaggggtgca
      901 taaactgcat cccttaactt atcttatttt gcttaaacaa tcttggcccg taaactgcat
      961 aactcatatg taaatcgctc ctttttaggt ggcacaaatg tgaggcattt tcactatttc
     1021 cggcaattac ttccatgtaa agtataaccc actatacttt gcaagcaaag tgtgggctcg
     1081 ccgaggggtc ttgcagacgg ctctttcggc tcttttgagc cgagtatcag cgcaccctta
     1141 tggtgcaatt agctgatacc tgtttgacga tttgctgccc taccttttcg gtaggtatgc
     1201 aaacctcaaa ccaaaataaa aacccccttc gacttcgtca gggggttttt atttatataa
     1261 atctccgtaa ataatcgcga tttaataaca tcttcaattt tatttttttg cgtattttag
     1321 aaatgttttc actaaaaaat atattataca aaaaatacag taatcgatta taattctttg
     1381 ccagttaaat atcatatcgt tattaaaata tcggaaaaaa atatcaacaa caataaatgc
     1441 aaaagaagac cataaaaatt gtttattact catacatact ctccatttct atcattttta
     1501 aatttaccag tcatctctca aaaatctcct agctattttt cttttttaac cacataccgt
     1561 ca
//
LOCUS       DAZUMM010000177         1539 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000177 DAZUMM010000000
VERSION     DAZUMM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.191"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1518)
                     /locus_tag="ACG8E6_002735"
     CDS             complement(52..1518)
                     /locus_tag="ACG8E6_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFS5063063.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          502 a          326 c          289 g          422 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aattcttta
//
LOCUS       DAZUMM010000178         1490 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000178 DAZUMM010000000
VERSION     DAZUMM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.192"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..905
                     /gene="scrK"
                     /locus_tag="ACG8E6_002736"
     CDS             33..905
                     /gene="scrK"
                     /locus_tag="ACG8E6_002736"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS5063064.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(1139..1490)
                     /locus_tag="ACG8E6_002737"
                     /pseudo
     CDS             complement(1139..1490)
                     /locus_tag="ACG8E6_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          441 a          282 c          330 g          437 t
ORIGIN      
        1 gatttcattc taaaataaag ggagaaatgt ctatgctata cggtgcaatt gaagcaggcg
       61 gcacaaaatt tgtgtgtgcc attggcgatg aagaaatgac gatcaaggaa agagtgagct
      121 ttccgacaac gacacctgag gaaacaatgc ctttagtcat tgacttcttc aaacaatacc
      181 aagcagattt agcaggaatt gggattggct catttgggcc tatcgatatc catcgcgatt
      241 ccgccactta tgggtacatt acctcgaccc cgaaattggc atggcaaaat tttgatttca
      301 tcggtaccat gaaaaaagaa tttccgatcc cgatttcttg gacaacagat gtcaatgcgg
      361 ctgcctatgg tgagtatgtc ttcggcagtg gcaaaggatt gagcagtgtc gtttactata
      421 cgatcggtac gggtgttggc ggcggtgcct tacaagaagg gcgctttatc gaagggttta
      481 gtcatccaga aatggggcat atgctcgtcg tccctcatcc aaaagatgac tttgctggca
      541 gctgtccatt tcatggcaac tgtttagaag ggatggccgc tggacccgcc atcgaaaaac
      601 gcttagggaa aaaaggacaa gaggttgcag aagatgatcc ttattgggaa atcgaagcct
      661 cctatattgc tcaatgtgcc tacaatacaa ccttgatgtt ttctccagat gtgatcattt
      721 ttggcggtgg tgtgatgaaa caacgccata tgctgcaaaa cgtccacgac gcatttgcaa
      781 aattggtcaa tgggtatgtc gaaacacctg agttaagcaa atatatcgtg accccagcat
      841 tagaagacaa cgctggaacg ctcggttgtt tggcattggc tcgagaagcg gtcttacatt
      901 cataaacgaa aaaaggctct tcgacattta gtgttggtag agaaagatac aactgaaaat
      961 ttagtcataa ccagttaggc agagtgctta actggttttt gttttacttg gaagatcaag
     1021 ccttgaatga gataaattct tgctcgaaag ttatggaaat ttcgatagcc gtaggctact
     1081 tgtattgtgc aatagttttt tacaaaaaaa aggggtgcgg atattttttt ggaccttttt
     1141 aacaaatata tctttgcttc ttccgatatt gaagagggct tagccctcct aaagataact
     1201 taattcgttg attattatac caatgaatat aattatccaa tttgtttata aactgattga
     1261 ttgttgtatt cttccagtca cgttgataaa acatctcgtt ttttaatcgt ccaaagaaac
     1321 cttcgcacgc tgaattatct ggtgaataac cctttttaga catagagtga agcaagttgt
     1381 ctttgttcca atagtccaac taacaatagc accatcgtaa caatcgacaa gtggtgataa
     1441 gtagattttc cctgcgggaa ttctaaattc agtaatatct gttaaccatt
//
LOCUS       DAZUMM010000179         1449 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000179 DAZUMM010000000
VERSION     DAZUMM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.193"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            679..765
                     /locus_tag="ACG8E6_002738"
     CDS             679..765
                     /locus_tag="ACG8E6_002738"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS5063065.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1067..1291)
                     /locus_tag="ACG8E6_002739"
     CDS             complement(1067..1291)
                     /locus_tag="ACG8E6_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063066.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT          434 a          296 c          230 g          489 t
ORIGIN      
        1 tccgtttttt catccattcc tttcttcccc ccttacaaat aaataataac ctttaacaac
       61 caactcgtaa ataggttggt tgttattata caaaaggata ttagtactca gataaaaagt
      121 tgttagtact cagataaaaa gttgttagta ctcagataaa aagttgttag tactcagata
      181 aaaagttgtt agtacccttc tgtatccgtt gggagagtaa ggctcaactg ccccctaaaa
      241 agggttctaa aaaatcttta aaaaataaat aaaataaacg cgcgcgcgcg agcaaaattt
      301 cccgccaaaa aatcacccac tttttaaaat aaacaggttc gcgcatgata ttaaactgcc
      361 aatctagctc cctatggtcg ctgtaagaag gagtgatcgc tacgctctca cattttactt
      421 gcagcagtcg cctttaggct ccttcgccaa aaataattca ttagggactc ctttgtggcg
      481 tcctgagagc tcgtctaagc cgttctaaga caagattcga taaaaactat gtccttcgtt
      541 ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac cccaaggcaa taaaaaactt
      601 aggcttattt ttgctttttt attgtataat tgattgtcgc ctatccgaca gtcttgcgac
      661 tggaaggagg tgtgtttcgt gttcactaac tttatttgct cccttcttgt tgggctttta
      721 agcgctttgt tcagttactg gttgaataac cgaaacaaga agtaaaactt aattaaggcg
      781 actcagccca caaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtgtttcg
      841 tgttcactta tttgcaatca aattataaca gaaataaata tttatttcaa aaatatccta
      901 caaaagtcta aaatccgtaa ttatttctta tatgcaacat ttacaaataa agtcgctctg
      961 agagctcgta tttttcgatt taagacgttt tatttagttt ttggtgtatt tcattggtcc
     1021 aagctaaaaa actcttagaa gcgattttag agccatttaa acccacttaa ttatattctc
     1081 catgaaattc gctccatgcg tcaaaagctg cgtctgaaat tccgccgtct tcgttcacta
     1141 gttgcattaa tttttccaac gatcgttttc tttcgggtgt catattttta tattccaaca
     1201 atttttcgtc tgttaaatcg aacttgattg cttgaattgc ttgctctaat ttttctaagt
     1261 tttgaaaata ttcgtaagga aatttttcca ttttttcttt ctccagtctc acaatttttc
     1321 tagaaataaa atcacttctt tgccgtctat actctcaaaa aaaatgtcgt ttgaaaaact
     1381 aaataaattt ttgatcgtgc atttttaaat ttggttatcg attacacgtg cagcactccc
     1441 ccccgaatt
//
LOCUS       DAZUMM010000180         1424 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000180 DAZUMM010000000
VERSION     DAZUMM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.194"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8E6_002740"
     CDS             complement(150..1337)
                     /locus_tag="ACG8E6_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFS5063067.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DAZUMM010000181         1420 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000181 DAZUMM010000000
VERSION     DAZUMM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.195"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACG8E6_002741"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACG8E6_002741"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFS5063068.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          496 a          184 c          236 g          504 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctattactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctggactaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta acttattgct tttgcaaaat ttatttacct gttttctgga tcaacgatag
     1141 tgtatattat gctatccttg tttggatttt aggacgttta aagaggcact tttttacata
     1201 acttaatcca tagacctttt aagcatgctg tgagataata ataaagaaga gtttaaagcg
     1261 caccccaaac cacttcccca taagtgtatt acgctttaaa ctcttttata tttgaattca
     1321 ctaagaagca taccatattt ttgaaaaaaa gtgagaaaaa aggcttataa ttaggattta
     1381 gagtaattaa ttagatgttt attttatagg actgatacta
//
LOCUS       DAZUMM010000182         1409 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000182 DAZUMM010000000
VERSION     DAZUMM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.196"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1359
                     /locus_tag="ACG8E6_002742"
     CDS             <1..1359
                     /locus_tag="ACG8E6_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFS5063069.1"
                     /translation="LFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKE
                     HLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSS
                     FKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNW
                     HIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPI
                     VYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPK
                     GKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDP
                     VAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEG
                     ARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFS
                     RN"
BASE COUNT          466 a          255 c          299 g          389 t
ORIGIN      
        1 ctttttagtg atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca
       61 gtaaaaggac atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata
      121 acagagaaag aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag
      181 atgaaagata tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg
      241 gattgtctca cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt
      301 tcttctttta aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat
      361 agtttagtgg cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa
      421 gagaactatt ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg
      481 catattaaag cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt
      541 aatcaagaag agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa
      601 acagatacct attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat
      661 tatcaccacg acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca
      721 gggtatttac actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc
      781 tattgtttcg cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac
      841 acggatattc ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag
      901 aaatggaaag acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca
      961 ttatttattg ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg
     1021 gatgcagccg ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat
     1081 ggtcgtttag aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga
     1141 cggaaaaatt ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg
     1201 agtgtatata aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta
     1261 tttgataaga taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct
     1321 tggaacaagg atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt
     1381 ctattctata ggtcgggtta tttgacgta
//
LOCUS       DAZUMM010000183         1387 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000183 DAZUMM010000000
VERSION     DAZUMM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.197"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            170..970
                     /locus_tag="ACG8E6_002743"
     CDS             170..970
                     /locus_tag="ACG8E6_002743"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5063070.1"
                     /translation="MRLRKQHYHYQSCGKYFTAHTYLVVPSCFISKQVHYTILEELTE
                     RQALKTLTKRCDLSVTTFQRTLSALSAMLRQMLDYLPHCLLFDEFRSLSISHGKFSFS
                     CMNRETGKLFDILSSRRKKDLIAYFMRFTRKAHMGVRYIVTNMNAPYFSLVKESFPNA
                     QIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKDTSQAKYYRQLKPLYKLLLKSDDELDY
                     TTFKKWQNFQWRYLTESEVVDRLPSISYELKIAKQFIKSCLAIITRNS"
     gene            1079..1225
                     /locus_tag="ACG8E6_002744"
     CDS             1079..1225
                     /locus_tag="ACG8E6_002744"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5063071.1"
                     /translation="MTKLENLRTHIKTLKRFAYGFRSFTNMRLWIFLINRLISYALKA
                     SNSY"
BASE COUNT          469 a          256 c          220 g          442 t
ORIGIN      
        1 ttttcaaaaa actttttata aggacaagaa agccatcgta atcgaaggta cattagttcg
       61 cacttttaga cattgtctat tttgtaaaga aactgcgaag cagctcgtga aaaacggcaa
      121 gaagatttca atgattatct taaatcgcag tgggaataaa taaacctatt tgcgtcttag
      181 aaaacaacat taccactacc aatcatgcgg aaaatatttt actgctcata cgtatcttgt
      241 cgttccttca tgttttattt cgaaacaagt tcattacacg atattagaag agttgaccga
      301 gcgtcaagcg ttaaagacac ttacgaaacg ttgtgatctt tcagtaacga catttcaacg
      361 gacattatct gctctatcgg caatgctgcg acagatgcta gactatttac ctcattgttt
      421 actattcgac gagtttcgct ctctttcaat ttcccatgga aagttctcct tttcttgtat
      481 gaatagagag acagggaagc tctttgacat tttgtccagc cggagaaaaa aagatttgat
      541 tgcttatttc atgcgtttta caagaaaagc tcatatgggg gttcgataca ttgtcacaaa
      601 tatgaatgcc ccttattttt cattagtcaa agagtccttt ccaaacgccc aaatcatcat
      661 tgattgcttt cacattggac aacacttgaa caagtttttc gatcgtgttc gaaaacgtgt
      721 gatgaagcag ttgaatcaaa aagacacttc acaagctaag tactatcgac aattgaaacc
      781 tttgtataaa ttacttctaa aatcagatga cgagttggat tacacaacat tcaagaaatg
      841 gcagaatttt caatggcgct atttaacaga aagtgaagta gttgatcgtc taccctcaat
      901 ctcttatgaa cttaaaattg ctaagcagtt tatcaaaagc tgcttagcaa ttatcacaag
      961 aaacagctag ctacttcgag aaacgaccaa agaagttccc tcgaaacttc actatctcaa
     1021 aaagcctttt tcatatctga aaaaggcttt taactagcat tttcgaaaaa gtattctaat
     1081 aacaaaatta gagaatcttc gcacacatat caaaacattg aaacgatttg cttatgggtt
     1141 tcgcagtttt accaatatgc gcttgtggat ttttctaatc aacaggttaa tcagctatgc
     1201 attaaaagct agtaactctt actaactttg ttgttcataa atgaccttat cagcccgatt
     1261 tgacaaagaa ctactttttt acaacattat tttattttcg agtaatcttt cttatttcat
     1321 ttcgtatatc tggaaaaaga aaataaacaa acggaagata aataatgaaa atcattattg
     1381 atttaat
//
LOCUS       DAZUMM010000184         1383 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000184 DAZUMM010000000
VERSION     DAZUMM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.198"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..684)
                     /locus_tag="ACG8E6_002745"
     CDS             complement(52..684)
                     /locus_tag="ACG8E6_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS5063072.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1035..1334
                     /locus_tag="ACG8E6_002746"
     CDS             1035..1334
                     /locus_tag="ACG8E6_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS5063073.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          500 a          194 c          218 g          471 t
ORIGIN      
        1 tttttatgga tatttgtatt gctaaaaatt tatcttatat attcattttg attatactac
       61 ttttatttgt aaaaattttc tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt
      121 ttttagatgt tccgagcgta cttcaatcat ttcaaaggag ttgctactta aatcgatatt
      181 ttttaaatca gtatcaaaaa agctagcatt ttctaaatca ttagtagaaa acattatttt
      241 agtgagggaa cagtctacaa aatctgcgct tgataagaca gagtgctcaa aggtactttc
      301 ttttatgttt gaaccagata aatttacata gtccatttta caattctcga ataacgtgtt
      361 cccaatataa ctttccatca atttagtacc tgttaattta caattgacaa atttcacgcg
      421 atgcaacatg ctttttgtca atatacagcc tgaaaaatca cagttttcga atatacagtc
      481 ggtaatctcc attctttgaa aatcattgga agcaaaaaat gcatacttga aaatagattt
      541 gtaaataatt ggatggcaga ttatttggtt ttcgatgacc tctttttgtg aaaggatatt
      601 atacagttca aaatcttcgt tttcatagat atgaaaaaag gatcctttat tcaagcttgc
      661 ttcattaact ttaggaaaaa tcatatttaa ttacctccat tttcataaag tcatattaac
      721 aaaagctaaa ccaacttaca ttagcagaaa atgagatagg ttttcgcctt tttcgataac
      781 gatagtgtcg ctatctcttg aaacgttctt agaattgccc tgagagacac aaattaggcg
      841 taattctaat aaaaatctta gaataaaaat ttttactgat ttaatttaga atgtttttaa
      901 aataaaaatc gttgccataa cagtgtattg aaaggtttta acaatccagt aatgatttaa
      961 aaaaatctag atgtagcgta tactttgttt atagaatgta gtgggaagaa atatattttt
     1021 gggaggaaat cgtgatgtct aaaagtgaag aaatagaaaa catggtacta aaattatatg
     1081 atctcgtttt aaatccagag atcaaagaaa aggaacgtat acttttgata gacgcaaaga
     1141 caggcttaga aaaaggtcag tattatccta aagtcataaa taatttggaa cgtagtttac
     1201 gtccgttagc aatcagggga gaattgtcta aaccagtatc accgttttat atggagatat
     1261 caacaatagg aaaatttgaa aaagaattag gtcgaggaat ggcttcggca ccgattactt
     1321 ttgggcattt ataaaaacga gttattcatc tcaatgaatt aaaaagtata gtcaatctca
     1381 taa
//
LOCUS       DAZUMM010000185         1368 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000185 DAZUMM010000000
VERSION     DAZUMM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.199"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..398
                     /locus_tag="ACG8E6_002747"
     CDS             6..398
                     /locus_tag="ACG8E6_002747"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5063074.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            399..>1368
                     /gene="aac(6')-Ie"
                     /locus_tag="ACG8E6_002748"
     CDS             399..>1368
                     /gene="aac(6')-Ie"
                     /locus_tag="ACG8E6_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HFS5063075.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTID"
BASE COUNT          562 a          110 c          230 g          466 t
ORIGIN      
        1 agtttatgga agaagtttta attgatgata atatggtttt tgatattgat aatttaaaag
       61 gatttcttaa tgataccagt tcatttgggt ttatagctaa agaaaataat aaaattatag
      121 gatttgcata ttgctataca cttttaagac ctgatggaaa aacaatgttt tatttacact
      181 caataggaat gttacctaac tatcaagaca aaggttatgg ttcaaaatta ttatctttta
      241 ttaaggaata ttctaaagag attggttgtt ctgaaatgtt tttaataact gataaaggta
      301 atcctagagc ttgccatgta tatgaaaaat taggtggtaa aaatgattat aaagatgaaa
      361 tagtatatgt atatgattat gaaaaaggtg ataaataaat gaatatagtt gaaaatgaaa
      421 tatgtataag aactttaata gatgatgatt ttcctttgat gttaaaatgg ttaactgatg
      481 aaagagtatt agaattttat gatggtagag ataaaaaata tacattagaa tcattaaaaa
      541 aacattatac agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg
      601 ttcctattgg atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt
      661 atccaaaaac tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt
      721 ggagtaaagg aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa
      781 gaaatgctaa tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat
      841 accaaaaatc tggttttaga attattgaag atttgccaga acatgaatta cacgagggca
      901 aaaaagaaga ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg
      961 caatgaaata tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa
     1021 tcggtagtgg ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa
     1081 aatttagtac taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa
     1141 atacaaattt agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg
     1201 aattatctat actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt
     1261 ctactatgtc agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac
     1321 aaatgcacgg tttagattat acagatatta gtgaatgtac tattgata
//
LOCUS       DAZUMM010000186         1337 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000186 DAZUMM010000000
VERSION     DAZUMM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.201"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1337
                     /locus_tag="ACG8E6_002749"
     rRNA            <1..1337
                     /locus_tag="ACG8E6_002749"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          358 a          293 c          406 g          280 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggactta
//
LOCUS       DAZUMM010000187         1309 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000187 DAZUMM010000000
VERSION     DAZUMM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.202"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..347)
                     /locus_tag="ACG8E6_002750"
     CDS             complement(<1..347)
                     /locus_tag="ACG8E6_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFS5063076.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAIL"
     gene            635..1288
                     /locus_tag="ACG8E6_002751"
     CDS             635..1288
                     /locus_tag="ACG8E6_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5063077.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          490 a          165 c          237 g          417 t
ORIGIN      
        1 atgagaatcg cctgcttcag ttcctttgtt agttgatgat gttcatcaac taagtccgtt
       61 ttcgcagaaa aggtctgccc acaatcatag catagaaaac gggtacgttt taattctaaa
      121 atagtcaact tttctttgac tttcaatatc tgagtacgtg tcgtatagga accgttgcgg
      181 ataatacgaa tcgatccaca gtgttcacaa tgggaaggtg agtcatccaa tctcccttta
      241 ataaactgcg cttgaacgcc tttatattct ttttcaatca gccattcttc atcaaaaaat
      301 aaatgtttat ctgttaatcc gagtaatttt ctagtatgat tatccataga gcgactcctt
      361 tggtagggtg atgttttggt cgactaaatt ttaccaaaag tgagtctctt tttgagttga
      421 aacgaagaaa aagtgttagt agaaaatcgc cttcgatttt ccaacaacac aaaatattat
      481 agaacccttt attctaaagg atttcaaggt gtctgtttgc atttattaaa aaaaagaaaa
      541 agtattggca acagaaggcg tcaccaacac tagatattat agagccataa tatgctgtat
      601 aatatctagt gtataggctt aagtggaggt atatatgaaa atattaactg ttgaagatga
      661 taatatgata agagagggaa taagtgaata tctttcagaa tttggatata ctgttattca
      721 agctaaagat ggaagagaag ccttgtcaaa atttaatagc gatataaatt tggttatctt
      781 agacattcag atacctttta taaatggttt agaagtgttg aaagaaatta gaaagaaaag
      841 caatttacca attctaatct tgactgcatt tagtgatgaa gaatataaaa ttgatgcatt
      901 tactaattta gtagacggat atgtagaaaa gccattttca ttgccggtct taaaagctag
      961 aatagattct ttaattaaga agaattatgg acatttagag aagtttgaat ataaggatct
     1021 atcggttaat tttaatagct atacagctaa aataaatggt gaagaaatag atgtaaatgc
     1081 aaaagaacta gaagtattaa aatgtttatt ggataataat ggacaagtgt taacaagaat
     1141 gcaaattatt gattatgtat ggaaagatag cgaagaaact ccttatgatc gagtaataga
     1201 tgtatatata aaagaactta gaaaaaaatt acaattagac tgtataacta ctataagaaa
     1261 tgtaggatat aaattggaga gaaaatgaaa aaattaaaga tatttccaa
//
LOCUS       DAZUMM010000188         1307 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000188 DAZUMM010000000
VERSION     DAZUMM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.203"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..1216
                     /gene="xerS"
                     /locus_tag="ACG8E6_002752"
     CDS             131..1216
                     /gene="xerS"
                     /locus_tag="ACG8E6_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFS5063078.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          522 a          218 c          194 g          373 t
ORIGIN      
        1 acctatttat ctgctttttt gaaataaatt aataaatttc aaagtatacc aattgacaaa
       61 acactctata attggtatag tccaattata gagatactcc tctcccctac ttatcgaaag
      121 gatgaataac atgaaaaata cagaaatata tcaaaaaata gatacaatca tacttcatat
      181 gcccgattat ataaaagatt ttgttcaaga tcgtgaggat aaagatcaat cacctcgaac
      241 tactttagaa tatctaaaaa attataagct cttttatgaa tggcttctga gtgagtctat
      301 cgttcccgat acttcaatca cctcgataag acagttaact gcctatgatc taaacttgta
      361 caaatctcat ttgaaacgtc gcacaaagga aaatgtaaaa aaagagacaa caaaaactaa
      421 gctcaaagat aataataact taggtctatc tacttctact atcaatagaa atatcacagc
      481 attaaaggtt ctatttaaat atctttcgaa aagttccaat aatcctttag gaaaaccata
      541 cttagaaaat aatcctatgg atcaagtagc aactatcaca gataaaacta cgttagctgc
      601 ccgtgcaaac gcaatcgaga aaaaattatt tctagacgaa gatacacaaa attatctaga
      661 ttatattgcc aatgaatata aaaaaacttt gagcaaacga gctttgatct attatcaacg
      721 agatgtagaa agagacctgg cgatcaatgc attgatttta ggatctggat taagactctc
      781 cgaggttgta aatattaatt tagaggacct atctttagaa aagaataatg tggtagtaac
      841 tagaaaagga aataagagag acgcagtaaa tatagcagca tttgcaatgg aatatttagc
      901 taattatcta gctattcgga aagaacgata caaagtaaca gaaaacgaaa aagctttatt
      961 tctagcaatt tatcaaggag aagccaagcg tatatccggt attgcaatcg aacggatggt
     1021 agcaaaatat tcaaaaggat tcagagtcca agtcagtcca cataaattac gtcatacttt
     1081 ggctactaga ctctatcaac agacaaattc attggtatta actgcccaac aattgggaca
     1141 tacaagtaca aatacaacaa ctttatatac tcatatagac aatgcagcta caattgatgc
     1201 tttgaacgct ttgtaaatgt agtaaaaaat agtataataa tgaagcctgt ctctaaaatt
     1261 agataaaagc ccaactttaa aggcaagctt cattatcatt tttatat
//
LOCUS       DAZUMM010000189         1256 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000189 DAZUMM010000000
VERSION     DAZUMM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.204"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..1158
                     /locus_tag="ACG8E6_002753"
     CDS             28..1158
                     /locus_tag="ACG8E6_002753"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFS5063079.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKE
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
BASE COUNT          457 a          207 c          245 g          347 t
ORIGIN      
        1 caaaaaacaa agaaaagagt gagtgaaatg agcaaaaata gaccatttac agtagatcat
       61 gtcggaagtt tcttacgccc gcaatcgatc aaagaagcaa gaaaaaaagt tcaagaaggc
      121 ttactttcaa aagaagaact tcgtgcaatc gaagaccagg aaataaaaaa attagtggaa
      181 gaccaaaaag cagtcggcat caaaggaatt acagatggtg aatttcggcg tggattttgg
      241 catcttgatt ttttagaaga actaaatggt atcgaaggct atgttccaga aactggatat
      301 aatcaaacat tccacggaaa agcagcacca gcttataata ttcgggtagt agataaatta
      361 tctttcaatc caaatcaccc atttttaaaa gattttgctt ttctgaaaga agtagtggaa
      421 acagatgata cttttgttgc aaaagcaacg atccctagtc ccacgatgat tttacgacaa
      481 gagatcctat ctaatgatgg ttcttcaaaa atcaaggaga tttatcctga tctatctgat
      541 ttttatgagg acttgtcctc tacttacaaa gcagcgattc atgcttttta tgaaaaaggt
      601 tgccgttacc tgcaattcga tgatacaaat tgggcgtttc tagctgatca aactaaaaga
      661 gaagcacttc ttcaaaaaga aattgatccg aaagaaatgg cccatatttg tacagagatt
      721 atcaataacg cactggaaga caaaccagaa gatttgacga tcacaacaca tatctgccgc
      781 gggaatcacg cttcttcttg gctgttttcc ggtggatatg aacccattgc agaagagttg
      841 ttttcaacaa attatgatgg tttctttttg gaatacgatt ctgaccgagc aggagatttt
      901 actcctttaa gacattggag taacaaagaa agtaaagttg ttttaggatt agttacttct
      961 aagtttccag atttggaaga tacggagaaa ataaaagaac gaataaaaga agcacaacaa
     1021 tttgtaccat tggaaaatct aagtatcagt cctcaatgtg ggtttgcttc aacagaagaa
     1081 ggaaaccatt taacagaaga agaacaatgg aagaaaatcc aattattgca gcaagtcgca
     1141 aaagaagttt ggctataaaa atgtgaggct tggcaaaaaa aagcgtccca aaaagtcggc
     1201 ttttcatgga gtggagaaat ccactctttt ttatttacct tttatattat ttattt
//
LOCUS       DAZUMM010000190         1249 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000190 DAZUMM010000000
VERSION     DAZUMM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.205"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..>1249
                     /locus_tag="ACG8E6_002754"
     CDS             13..>1249
                     /locus_tag="ACG8E6_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFS5063080.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFK"
BASE COUNT          417 a          247 c          242 g          343 t
ORIGIN      
        1 acgatacgct atatgaatga ttctatcaaa aaaatgctga gaataacaga gaaagatttg
       61 atgattacag aggtctctta cgagaccctt cagaagaaaa agacgttggt cgtcgatgct
      121 gttctctcgc ctactcctcg tgcttgtaga agttgtggtt ctactgtggt agatggaaac
      181 gggaaagcaa ttatagtgaa aaatggaaaa aaggaaacga ttgtccgttt tgaacaatac
      241 aatcatatgc ctttggttat gcgcctaaaa aagcaacgct atacctgtaa aaactgtcgc
      301 acccattgga cggctcaaag ttattttgtc caacccagac attcaatcgc aaatcatgtt
      361 agatataaaa ttgcttcttt actgactgaa aaagtatctt tatcttttat tgcgaaaagc
      421 tgtcaggtat ctttgaccac cgttattcgt acattgaaag agtttaaaag ctatttacca
      481 aagcaatcta agaagatcct ccccagagta ttgatggttg acgaatttcg ttcgcatgct
      541 tccatagaag ataagatgag ctttatttgc gcggatggcg aaacaggaaa attaatagat
      601 gttttgccta cgcgtaaatt acctcgatta acaagctatt tcttaggttg taccaatcca
      661 gaagaagtag aattcttggt gacagacatg aacgccgcct acttccagct caccaaacgt
      721 gttctgccaa atgcgaaagt cgtgattgat cggtttcata ttgtcaaaca catgaatcaa
      781 gcgttcaatg agttgcgtat ccgtgaaatg aatgaacttc gtaaagccgg acagaaaagc
      841 caggcagaaa aactgaaaaa gaactggcgc tttttgctaa aaaatcgtgc aaacatcaac
      901 cattatgaat acaaaacatg gaaaagtttc cgagcaccaa aatacccatt tcttactgaa
      961 gcaatgatga ttgatcgatt gcttgagttt tctccgcccc taaaggaggc gtatcccttt
     1021 tttcatgaat tagttgaagc ctttcgagac aaagaccctg acttattttt ctccttattg
     1081 gcagaacttc ccgaaacgtt agatgacagc tttcgggaaa agcttcaaaa ccttctgacc
     1141 tatgaagaag gcatcaccaa cgcaatgatc tatccttatt ccaatggaaa aatagaagcg
     1201 aagaataccc acataaagac aatgaaacga gtatcctacg gatttaaat
//
LOCUS       DAZUMM010000191         1248 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000191 DAZUMM010000000
VERSION     DAZUMM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.206"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACG8E6_002755"
     CDS             200..742
                     /locus_tag="ACG8E6_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063081.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFKKNTSEEKNLKL"
     gene            898..>1248
                     /locus_tag="ACG8E6_002756"
     CDS             898..>1248
                     /locus_tag="ACG8E6_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFS5063082.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPD"
BASE COUNT          515 a          141 c          237 g          355 t
ORIGIN      
        1 attgatgaag cagttcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttaaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagat
//
LOCUS       DAZUMM010000192         1208 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000192 DAZUMM010000000
VERSION     DAZUMM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.207"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(325..681)
                     /locus_tag="ACG8E6_002757"
     CDS             complement(325..681)
                     /locus_tag="ACG8E6_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5063083.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(695..1105)
                     /locus_tag="ACG8E6_002758"
     CDS             complement(695..1105)
                     /locus_tag="ACG8E6_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5063084.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          387 a          225 c          169 g          427 t
ORIGIN      
        1 caagtggttc ttttttaaca actcagttat cttatcatgt catttctaac atgtcaacaa
       61 ctattttata tgttttttaa aatactattt tattgctttg ctacttaaaa caacagaaat
      121 gaatatatca tgtaaattta cacatgtcaa tatattttta actctatatc cccacctaga
      181 tttgaatttt gaccacataa attgagcatg tctctatagt agaaaaataa agtcaaaaaa
      241 agaacacata acacgcttga tgctaagtgt tcgactgtta caactgtatg aaatgctcag
      301 tgacttgatt aaaacagtta aagtctattt atttaagata agctgttcca tcaactgctc
      361 attgtatcca ataaatagaa cttcatcgat aataatcaaa ggacgtctga ggagattggt
      421 atgttgtgtg cataaaaaaa tcagttgttt ttttcctaca caggaaaggt caatcgcaat
      481 gttgtgaaaa gcctcagacc aagtcgcaaa tagctgttct gcttgcactt cttcaaaact
      541 gcataattct tctgcttctt ctgtacttag atcgcgttct tctaagtcaa caaattgata
      601 aggcaattga tggtgttcta accattgttt cgccttcaga cttgttacac agtgcgctga
      661 tccatagact ttcacttcca cgaaggctcc ctccctatga atttaagatg ttattgtttt
      721 gtaaatagat cagatttctg gcaacacatc gaatttcacg agggacaaat gaccggatct
      781 catcttcatt aaatccgatt agtattcttt tttcatcaat caaaataggt cgtctcaata
      841 atgaaggata ttctttgaat aagacgatca tttgctttaa agttaattcg ttaaaatcta
      901 tgcccagctt ttcgtaaatt ttcgaccgag tggaaataat atcttgaact cctccttcag
      961 tcgctgccaa gatctttttg atctcacttt ctgttaacgg ttcggcaaaa atattcttct
     1021 cttcaaattc aagttgattc gcttgcaacc atgctcttgc ttttctacag gaagtacagc
     1081 taggcgaagt atatactttt atcattttct cactcctttt taattataat ccaattctac
     1141 agtaatttct tttaaaatac caattattag ctaataaaat ataaaaaaaa tcaaaataac
     1201 tgattatt
//
LOCUS       DAZUMM010000193         1202 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000193 DAZUMM010000000
VERSION     DAZUMM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.208"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..1061)
                     /locus_tag="ACG8E6_002759"
     CDS             complement(21..1061)
                     /locus_tag="ACG8E6_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS5063085.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          346 a          200 c          173 g          483 t
ORIGIN      
        1 atcactttgt cagtaaagtt tcactctaac cagtctttcg taatagtgct tggggagctt
       61 tcataattca aacgtttttc acttgatact ttagcagcta ttggtttttc gataaaccaa
      121 ttttttacat agacaaaaat caggttctgg atattttcga ctttttcggt ttttaatttt
      181 tgataggctt ttaacagtgt cgtgtataat tcttgttctg cattgacata ataactatcg
      241 agttcttcaa aaactatcga aataccactt tctatttcag cttttttctt tgcattatga
      301 atagtctgga cggttttttg agcttcactg taacttgaag aaaatgtttt tatcaaattt
      361 aatactcgtt cagggacaaa cgtagcaatg ctgtcgtttg tcataattgt ctcaaaattg
      421 tctaacaaca attgatcttg taggcggtct ttttcagtgt ctatattgtg tctattagtg
      481 tctaatatat tattattaag ttctggtatg attttcatac cctctgattt atcaacgttt
      541 tggcacggta tgattttcat accctcttga gccccaaggg attcagcgtc tgatttttcg
      601 tcgagggtgg gtatgatttt cataccctct gatttatcaa cgttttccct attctctcta
      661 tcaaattttt cgagtaagta gatatcattt tctgagactt ttaattctgc tagatatagt
      721 ctattaggat tatttttccc taactgtttg ttaaatccca tctgcttttg taataataaa
      781 ccaaaggatt ccaactcttt tttgattgct atcgcttttt gtttttggca gtttaatacg
      841 ctacatagtt catttgtcgt ataagtaaaa taaacattac cttcttcatc gatttgccgt
      901 ttgctgattg cgatctctaa tcttgctttt aaaagcatat aggcaatctt agttgaatct
      961 ttcatttttt tgtattcgtc agaaacaata aagacttttg gaaattgata aaataattcg
     1021 ttataaactc tatccgcctt aaaaaaatta aaatcagtca ttgtagatcc tctactttca
     1081 cgttgtttta tttatgtaaa attacctact cttttcgaaa atagcaaaga taaataaaaa
     1141 atgcaaacgc attaaaactg ttttttaatt aaatactacc taaaatcaga aataaaatga
     1201 at
//
LOCUS       DAZUMM010000194         1193 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000194 DAZUMM010000000
VERSION     DAZUMM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.209"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACG8E6_002760"
     CDS             <1..1076
                     /locus_tag="ACG8E6_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS5063086.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAZUMM010000195         1180 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000195 DAZUMM010000000
VERSION     DAZUMM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.210"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..948
                     /locus_tag="ACG8E6_002761"
     CDS             <1..948
                     /locus_tag="ACG8E6_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HFS5063087.1"
                     /translation="IITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTIKYAKRKK
                     QLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKKYVSAFNI
                     VPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQLEWLGVDN
                     LREFFKKQQQELNFMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLEMLFEEID
                     VLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDSYGLVDVF
                     KNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDIK"
     gene            945..>1180
                     /locus_tag="ACG8E6_002762"
     CDS             945..>1180
                     /locus_tag="ACG8E6_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5063088.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALS"
BASE COUNT          440 a          151 c          193 g          396 t
ORIGIN      
        1 attataactg atttaccaga ttatattaaa agaattgact ccgattcttt tttagctgcc
       61 ttttatttat atacagctaa gtattggata gacgatagtc ggaaaacgat taagtatgca
      121 aaaaggaaaa aacaacttta ttttcaaaca tggcacggaa cgcctttaaa aaaaattgaa
      181 tttgatgcaa aagataagtt acctaaacgt tacctaagct atgcgaaaaa agatagtgag
      241 tcaattacat atttattatc tggtaatcgc tatagttcta agaagtatgt ttcagcattc
      301 aatatcgttc cttctaaaat attagaagta ggaactccta gaaacgatga attagcctct
      361 agtaaagagc aagtgtttga cttgaaaaag aaacaaatca atgtcttatt tgctccaact
      421 tttcgaaata atattgagga caacggaatt acgcaactag aatggttagg tgtggataac
      481 ttacgtgaat tttttaaaaa gcaacaacaa gaattaaatt ttatgactaa atttcatcct
      541 aatgttcata gtaaattagc aactgattct aaaagtatag aatatatgaa aaaacatcaa
      601 attactttaa ttaatgatgg gattcctctt gaaatgcttt ttgaagagat agatgtttta
      661 atcacagatt attcatctat ttttttcgat tttgcgctaa caaaaaaacc aattattctt
      721 tttaactatg atgaaaaaga atatagctta gaaagaggtt tttatataca gttaagtgag
      781 ttaccaatta taagcgtaca agatagttat ggccttgttg atgtttttaa aaaccaacaa
      841 gaaaagcttt tgaaaagttc tcaagagtta cttgattata ttggaaatta tgaaacagga
      901 gcaagtacag aaaaggttct tgagttgata gagagggata taaagtgaaa aaaacagtaa
      961 aagatatatt ttataatgcg atatatcaag tctttttgat tgttttaccc ttacttacta
     1021 tacctatact tagtcgtaga attggttcaa ctggtttagg gatttatggg tatgttttct
     1081 caatatcaca gtttttaatg actgtcattg ctgtgggaat gaatcctttt cgtattcgaa
     1141 atatcgctaa atcaaggaaa gataaaaaag cactatcttt
//
LOCUS       DAZUMM010000196         1150 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000196 DAZUMM010000000
VERSION     DAZUMM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.211"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            454..>1150
                     /locus_tag="ACG8E6_002763"
     CDS             454..>1150
                     /locus_tag="ACG8E6_002763"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5063089.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKT"
BASE COUNT          416 a          188 c          190 g          356 t
ORIGIN      
        1 tttatttctt taatagtaat gttttctgtc attttaaata atactgttct ttattataac
       61 gcttattttc tctgttatat aagcgtagta ttttaaagca atgattacta aataaaattt
      121 actataaaca aataaatcaa ataacgttat tttatgtttg aatagttata ttgagactgt
      181 attaaaaaaa taaatctttc gaaaattcca cagagattgc attaaaataa atcaatcgac
      241 ttctaccata atgattaatt tgttcatcaa acttattgac gtgtttcacg atttttagtg
      301 aaaaaaactg aaatataaaa accttattct gtcaaggaaa tagaaatata gtaagaaaaa
      361 cctttattta tcagcttttt tagacaattt tttctgttac aatagtcaag gatttgtgaa
      421 aaaatcaatg aaaaccgaaa ggcgtgtaaa attttgatta aatcagttgt gtttgatgtt
      481 gatgatacga tgtatgatca gcagcagcca tttcgaaacg cggtcaaaag agtcgttccg
      541 cttgtttcag atgcagatat gcatccacta tatatccggt tccgccatca tagtgatgaa
      601 aacttcccaa aagtaatggc aggagattgg acactagaat atatgcgagc acatcggatc
      661 agccaatcac taaaagattt agactaccct catattacgg aagaagatgg cctcttattt
      721 caaaaaattt acgaggaaga acttgataat atctgcttgc atgaggaggt caaaaaaacc
      781 ctcgatttct tgaaggaaaa gaatgtccca ttaggaatta tcacaaatgg gccgactgac
      841 catcagacaa aaaaactgaa gcaacttcaa ttgaacaatt ggattccttc ccgcaatatg
      901 ctcatttctc aagcaacagg tttccaaaaa ccagaaaaag agatttttca gctagctgag
      961 aaagaatttc atatgcttcc agaagagacc ttatacgttg gcgataatta cgacaatgat
     1021 gttctaggtg caaaaagtgc taactggcaa gcgctttggt tcaaccatcg agaacgcaaa
     1081 attgaacaga ctccaatttg cgatatcgag atttcttctt ttgatcagct gttagagaca
     1141 atgaagacaa
//
LOCUS       DAZUMM010000197         1139 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000197 DAZUMM010000000
VERSION     DAZUMM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.212"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(295..368)
                     /locus_tag="ACG8E6_002764"
     tRNA            complement(295..368)
                     /locus_tag="ACG8E6_002764"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(332..334),aa:Ile,seq:cat)
     gene            complement(558..>1139)
                     /locus_tag="ACG8E6_002765"
     CDS             complement(558..>1139)
                     /locus_tag="ACG8E6_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS5063090.1"
                     /translation="LDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYD
                     LAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIENANLHVAGWHI
                     ANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKV
                     YVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          348 a          212 c          201 g          378 t
ORIGIN      
        1 ttcaaatata aaagagttta aagcgtaaca cacttatggg gaagtggttt ggggtgcgct
       61 ttaaactctt ctttattatt atctcacaat ttaacccaaa tgtctttcta tttaaaaatc
      121 aaagtaaaac ttttcaaata tacagaagta taactatgtg aaacatcctt tcattaatcc
      181 ataaaaggat acataaaaaa gccactcatt tgagtggcaa tgaagaaaag ctttagcttg
      241 tataatactc ttcaaaaaaa ttctaacaca gaacgattca aatggctacg ttaatgtacc
      301 ctgtaggact cgaacctacg accggacggt tatgagccgt ctgctctgac caactgagct
      361 aagggtactg gttgttgcca cataaagcca taaacaatca accagtagaa tgtgtggcaa
      421 caaacctgtt atcgcatagc ttggagtgtg actatttatg ggtgatagtg aagatatgcg
      481 ataacatcac tattttatcg aataattttt atagttgtca atatagttat gtaatgctcc
      541 tcaacgagga gctattttta tcgtttagga atatttaaat accaacgttt gtcatggaaa
      601 tcttgcgcac cgcctttagt gtttccctct ggatcattcg ttgcccgcat catgacgtat
      661 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      721 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      781 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      841 catccagcta catgcaaatt tgcattttcg atttctccaa actgatcaat gtgggcgtaa
      901 ttcgttccat ctgtcagtgt aggattagct gcaccagctc gtgttggatc aatgacaggc
      961 ttgttttcag aagttgttgg attttcatcg gtaaatccat gagctaaatc atatgctaat
     1021 ttttctttac ttacgcccat ttcagaaaga taaccgtatg gatcagtatg atctccccag
     1081 atattttgtg ttacccataa atgtgatttg attcctggtt ggttataagg cgtgtctaa
//
LOCUS       DAZUMM010000198         1102 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000198 DAZUMM010000000
VERSION     DAZUMM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.213"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..834
                     /locus_tag="ACG8E6_002766"
     CDS             301..834
                     /locus_tag="ACG8E6_002766"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS5063091.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          355 a          205 c          202 g          340 t
ORIGIN      
        1 attttttgag ctaataccga gggtcagctg attgaacacc gtttagtgga gaagttgtga
       61 aaaagtcgcc tactttcgag tcgtaagaaa tttttttgaa aatggttcct cacaactttg
      121 cataaatttg acttaatacc gagaagtcat gatccccatc taactccaaa tcacaaggaa
      181 tgagttcaaa aactagcttt tctcaacaaa tattacataa aaaattctat gttttttgtt
      241 aaatttattt gcttttttca aaagtatgtt acagtaattt cccaaggagg ttttacagaa
      301 atgaattttg ttgccatgga ttttgaaact gccaatcatc agccttatag tgcttgttct
      361 cttgcgctag tcatggtaaa aaatagccag atcgttgatg aattctatac attgatacag
      421 cctgagactc cttttttctg gcgaaatgtc caaattcacg gaatccatca agaagacgta
      481 cgcaatgcgc cgaaattccc agaagtctgg caaactatcc aaccatattt tcaaaaaaat
      541 cgtttagtcg ttgcacataa tgctgctttt gatacaaagg tcttagcggg atgcttagat
      601 tattatcatc ttgaacagcc taattatctt tctttatgta cagtgaaaag tagccgtcgc
      661 ctgtttcctg aatttccaaa tcatcgattg aacactgtgt gcgaaaattt gaacatccaa
      721 ttagatcatc accatgatgc attggaagat agtcgtgcat gtgcggaaat cctgctttat
      781 caagaaaaaa aattcgggac agatccattg aaaaaacttg tgactataaa gtaaaagaat
      841 acttatgaaa cacacaaaga ggtcgtgagc ctaccgtttc actccgagtc acaaggagca
      901 atttcaaaaa atagcttctc atgtttatta aatattgtga ctggtgaccg aggagctggc
      961 aggagaacac cgtttattgg agaggttgtg aaaaagctgt tctgcatcaa gtaataagaa
     1021 cagccaaaca aaaatagctt ctcatatttt tcatttggtt ggacttatta ccgcagatgc
     1081 aagcttttgg acaccgttta cc
//
LOCUS       DAZUMM010000199         1081 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000199 DAZUMM010000000
VERSION     DAZUMM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.215"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..860)
                     /locus_tag="ACG8E6_002767"
     CDS             complement(51..860)
                     /locus_tag="ACG8E6_002767"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5063092.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLNNQEDGLAVYLEKLLKL"
BASE COUNT          330 a          203 c          190 g          358 t
ORIGIN      
        1 gttgtgacaa aaattgtcac aacctctttt ttgtggaatc taattctcta ttatagttta
       61 agtagttttt ccagataaac agctaaaccg tcttcctgat tgttcaaagg agtgacatca
      121 tttgcaatgc ctttgagttg atcagtaccg ttagccatag ctactcccca acctgcataa
      181 tccaacaatt ccaaatcatt atgctcgtca ccaaaggcaa tgacgtcttt ttgatcgata
      241 gacaggtagt tagcaatctc ttgcacgcct tttgcttttt ggattccttt tgaaacaatc
      301 tctaagatgg cagtaggtcc accccatgtc cgaacatcga cataactgcc aaattgacgt
      361 gttaattcag cagatacagc tcctgcaaaa cgtttgtctg ttcggacaag caaagatgta
      421 ggatttgtcg tcaaattttt aggagaaagc aaattcttct ctcctggacg agaagaagca
      481 aaaatttttt catcaaagaa atcaaaactg tcaataaaaa aagtatcacg attttcagca
      541 gcgataaaat ctaaatttag ctgctttttt tgcgataaga tttcaaaagc gatttcacga
      601 ttgatcaacg tttctctttc accatcccag tgctgattag ggatgtgtac taatgcgccg
      661 ttaaaattaa ccatcggtgt tgttaattct aattgttggt aaaactgatg gctcattcgg
      721 aatgggcgtc ctgtagcaat actgacatga tgaccggcat tgattgcttt tttcagagtc
      781 tcttttgttt tagatgtaat catcgaatct tgattgaggg tagttccgtc cagatcgatg
      841 gcaattaatt ttttattcaa taagggtcct tccttcctca actagcattt gttttagtct
      901 agcatatttt aggtaattaa caagagcaat cagctattcc taacaaaaac tacagttatg
      961 gctatataaa tagctaaaca taagctaaac acgaacgtta agtattattt tcgctttttt
     1021 tgttaaaaaa acgttgaaaa gcgaatgaaa cgtctatata atttgaaatt gttcgatgga
     1081 t
//
LOCUS       DAZUMM010000200         1061 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000200 DAZUMM010000000
VERSION     DAZUMM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.216"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..995)
                     /locus_tag="ACG8E6_002768"
     CDS             complement(39..995)
                     /locus_tag="ACG8E6_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063093.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          381 t
ORIGIN      
        1 ttttagaaaa agcagtcgga ctttctaaaa gaagttcact attcgatcct gtcgatgatt
       61 tcttcaggaa aatcgtctat ttgcttgtca attttgctgt ctttgtattt ctcttcttgg
      121 atagtcaaca aaatctttgt aagattcccc cagtctactg ctcttggcgg atcgagccaa
      181 accactactt gagatgcact ttcttcatat agattattcc tattcgtgat tataacatcc
      241 gggaactcag gctttcctgc ctctatcact tccgtgttca gattaatgta caagcttaaa
      301 tttttcttga taaattgatt ataggcagca ccataagaaa aatcgatgat gatctttact
      361 ggttcagaaa tcagttgaat gggtatgtga ttgatcaata ataataaata actgaaaaaa
      421 agaaaaaatt tatcttgaag caactctttg cgttttgacg ttagatcatt tatatactct
      481 atcaaatagc aataaaatgc tggatagttt tctctaaaaa aagaaacatc aagtggaaat
      541 tcatcagctt tattaatagt gtaatgcaaa agtttaaaat gtattttggt taactctctt
      601 cggatcaatt ggctcgtctc ttctcgcaac agattaaact gattttcaaa actttcaatg
      661 aatcgttcat tcaaccattc gattgtttca tcttcatttt ttctcaagta tttttgatcc
      721 aatttatcat gaagataaag ccaagagagt gtcttattga taaaaataac ggatttttct
      781 cctaatactc cttttaagtg gtaaaatcct atatccgaaa aaaatagcga ctgattgcta
      841 gcaatatcta aagaatcagc ttgttcagcc aacaggttga ttgctaaaaa gtggaataat
      901 ttcttttttt cataagcaga cagaggataa tactgtttta aatcttttaa cattttttct
      961 gtttttataa ttataggtag cgtatacaat tccacaaaac ttatctctgt tgagttaaaa
     1021 agattatata aaaaaagccg aatgttttta ggattccctg t
//
LOCUS       DAZUMM010000201         1041 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000201 DAZUMM010000000
VERSION     DAZUMM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.217"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            287..694
                     /locus_tag="ACG8E6_002769"
     CDS             287..694
                     /locus_tag="ACG8E6_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063094.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..>1041
                     /locus_tag="ACG8E6_002770"
     CDS             708..>1041
                     /locus_tag="ACG8E6_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063095.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLD"
BASE COUNT          390 a          130 c          179 g          342 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat a
//
LOCUS       DAZUMM010000202         1002 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000202 DAZUMM010000000
VERSION     DAZUMM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.219"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..434)
                     /locus_tag="ACG8E6_002771"
     CDS             complement(<1..434)
                     /locus_tag="ACG8E6_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5063096.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..855)
                     /gene="tnpA"
                     /locus_tag="ACG8E6_002772"
     CDS             complement(451..855)
                     /gene="tnpA"
                     /locus_tag="ACG8E6_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFS5063097.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          304 a          173 c          163 g          362 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga
      541 ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt ttatcaatct
      601 tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag gttgagcttt
      661 gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa tgtgatacga
      721 ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat caatcacttg
      781 tctacgatat ttcgttacta aaacaaggtg gtaataaaga agaaatactg aatgattatt
      841 tgtatctaat tccatgttat atcaactcat ttcttgtata gactgattgt aacatggaac
      901 aaaataaaaa ggcaatacgc cttagttgtc ggtcttcgag ttaccaacgg taaccctcat
      961 accgaacggc attcatcacc cacctataga ggatgggcga ct
//
LOCUS       DAZUMM010000203         1001 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000203 DAZUMM010000000
VERSION     DAZUMM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.220"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..635)
                     /locus_tag="ACG8E6_002773"
     CDS             complement(81..635)
                     /locus_tag="ACG8E6_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5063098.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          324 a          185 c          107 g          385 t
ORIGIN      
        1 cttccgaaaa ttcagttatc ttcattaatc tcaccttcta agttttcatt atacacaaaa
       61 aatatatcct aatatctcaa ttagcctccc aatttaaaac cacgtaacga tgcccctatt
      121 tcatcttcag ttataccaat gtaccttttt gtaattgctt gcgagctatg attaaaaata
      181 aacattaagg tagcaatgtc tctagttttc ttgtagtagt gatacccaaa agtttttctt
      241 aatgtatgtg ttcctatatc atctcttcct aatttttctg ctatatctgc atatatctga
      301 tagacacgat cccttgttat atgtcgacct tgttgccgac ttggaaataa ccaatcattt
      361 tcttctttac cttcagtata taataaaatc tcttctctca aattatctat aaaaatcggt
      421 tttcgcttac ctgttttatg ttcaacaata ataggcttac ttttacctcg tatgtctcca
      481 acttttaatc ctataatatc tgacatacgt aacccattat tgattccaaa caaaaataaa
      541 aaactatctc tttctggatt acttgttctt cttagccaaa actgaaaatc ttgtatctct
      601 tcatttgtcc ttaacggttg tacattatag ctcactattg tttcccttct taaaaatatt
      661 ctgagttcat gattatatat atattttacc ataatcatga acttatatat atactctgtt
      721 aaaagtgaag aaaatcaagg ggtttagttt atgattttat atcatttcag taactcagaa
      781 tattccgact tttttgtaca aaaaaattaa ccttacttat atgaagattc taagctctta
      841 tttaaatttt agacgggcag ttatccgtag aagttcttag aatagctaat ttacataatt
      901 aaattatatg aaatttaagt ttgaacaata tactaagaac tccccttact ataaagtgaa
      961 ataaattagc atatgaataa ccgtgctacc aaacttttca t
//
LOCUS       DAZUMM010000204          943 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000204 DAZUMM010000000
VERSION     DAZUMM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.221"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACG8E6_002774"
     rRNA            <1..>943
                     /locus_tag="ACG8E6_002774"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAZUMM010000205          937 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000205 DAZUMM010000000
VERSION     DAZUMM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.222"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>937)
                     /locus_tag="ACG8E6_002775"
     rRNA            complement(<1..>937)
                     /locus_tag="ACG8E6_002775"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          198 a          291 c          210 g          238 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctc
//
LOCUS       DAZUMM010000206          926 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000206 DAZUMM010000000
VERSION     DAZUMM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.223"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACG8E6_002776"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACG8E6_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS5063099.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          269 a          192 c          139 g          326 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa ctctgtttgt tcatcatgat atagattatc
      901 ttttgcttta aaaaaaacca attatg
//
LOCUS       DAZUMM010000207          911 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000207 DAZUMM010000000
VERSION     DAZUMM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.224"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..>911
                     /locus_tag="ACG8E6_002777"
     CDS             28..>911
                     /locus_tag="ACG8E6_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5063100.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPR"
BASE COUNT          317 a          157 c          170 g          267 t
ORIGIN      
        1 ccaaagaatt ataaggagtg agtacaaatg acctataccc atcttacaac ggatgaactt
       61 gtaataatag agtcttattt caaaatgaat caatctgttg ctaaaactgc ccattgcttg
      121 aatcgttcaa gacaaacgat ccataaagta tatctgttct tcaagcaagg aaaatcagcc
      181 ttagaatatt atcaacagta taagaaaaac aaatcaaact gtggtagacg tccgcttgtt
      241 ttacccgagg aacaatcaga atatattcaa agaaaggttg ttcaaggatg gacacccgat
      301 gtgattgttg gtcgtgcagc gtttcctatt agttgttctg ctcgtaccat ttatcgtatg
      361 ttcaaaaagg ggctatttga ttcttctgac ttaccgatga aaggcaagcg taaaccgaat
      421 ggacatcaag aaagacgtgg aaaacaaact ttccgccgct ctattcatga acgtgaaaag
      481 gattatagcc aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtctg
      541 aaacataaaa gtgctgtaat taccttagtt gaacgattat caaaagttat catcacattg
      601 aaaccgtgtg gtagacaagc gattgatatt gaaaaaaaat taaatcaatg gtttgaatct
      661 gtaccgaaaa acctattcaa atccatcact tttgattgtg gaaaggaatt ttcaaattgg
      721 aaacagatca gtaatgtcaa tgatattgcc atttatttcg ctgatccagg aacgccgtct
      781 caaagaggcc taaacgagaa ttctaacgga ttgttacgta gagatggttt attgaaatct
      841 atggatttca attcagtaga tgaatttttt attcaatctg tcgcatctaa acgaaataat
      901 attcctagaa a
//
LOCUS       DAZUMM010000208          890 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000208 DAZUMM010000000
VERSION     DAZUMM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.225"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..159
                     /locus_tag="ACG8E6_002778"
     CDS             <1..159
                     /locus_tag="ACG8E6_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS5063101.1"
                     /translation="RRNKFYRSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
     gene            242..859
                     /locus_tag="ACG8E6_002779"
     CDS             242..859
                     /locus_tag="ACG8E6_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063102.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
BASE COUNT          313 a          111 c          172 g          294 t
ORIGIN      
        1 cgacggaata aattttatcg aagtctccgg actgcctcat ccacgattaa ggggatggag
       61 accattcgag gaatatataa aaagaaccga agaaatggaa cgctcttcgg cttttcggtg
      121 tctactgaga tcaaggtatt aatgggaata ccagcctaag atatttggag ttcagagagg
      181 gcgcgtttga ttttcaaact tcgcaacaga accattattt taaagtggga ggaaaattgt
      241 tttgtggata tttaaacaag aaatctttgc taaattattt agtttagatg gattaggttg
      301 gggaatctta ttttttattt ttttattttt aggatctatg tggatctggt ttgttgataa
      361 aaaaaattct tatgagaaag taatagtgaa aaaaatgaat cgaaatgaat tcgatgattt
      421 ttcaaaggaa catagagttg aattgtacga tagtcatgaa attaacagta cttctccatc
      481 ttattattgg gtatttggtt gcgaagaaaa accattggag attaaattgt tgaaaatgct
      541 tgaaaatcct ccagtcattg aagatttttc tgcaaaaaac gttgaaaaac agatgtttga
      601 acaggtacaa ataagaggtc aaaacaaaat ttcattagaa ccagatgaat atgcagcctt
      661 ttttttagaa acaaattcag gtacttcaat gcctgttttc aaagttaaat taaaaatgaa
      721 tggtcgttta tgcacttatg atcttgttgc aaatatgcgc tatggttcta ctggtgatag
      781 acgtactatt ttaaaatttg agcaaacctt tagaagtttt ttgaaaaata gagtatccca
      841 aatttttaat ttggattgaa aaaattaaaa atttggccaa aaatactaca
//
LOCUS       DAZUMM010000209          887 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000209 DAZUMM010000000
VERSION     DAZUMM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.226"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>887
                     /locus_tag="ACG8E6_002780"
     CDS             <1..>887
                     /locus_tag="ACG8E6_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5063103.1"
                     /translation="WFFQGIEEFSKVVIRNTVIKLSSVLLIIIFVSTKDDLWIYVFIT
                     SITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLFMQVYTTLDKTIVG
                     YFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKSENILVYTKKSFDY
                     TIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVSPIGGVFANQFAIA
                     IEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLVFFFRVFFVRKEIK
                     ISYFFGKNVILFFIVNLTLT"
BASE COUNT          273 a          115 c          156 g          343 t
ORIGIN      
        1 tggttttttc aaggaattga agaattttcg aaagtggtta ttagaaatac agttatcaaa
       61 ttatcatcgg tattgttaat aatcattttt gtttcaacta aagatgattt gtggatttac
      121 gtgttcatta cttcaattac taacttttta ggaagtttag ttttctggtt aagtatccac
      181 ggaaaagtag gtaaaccaat ctttaactct aatgttttta aacagttgtg gaaaccagct
      241 tttattattt tgactcctca gttgtttatg caagtgtaca caactttaga caaaacgata
      301 gttgggtatt ttgtggatcc aacggaatta tcttattatg atcagtctca aaaaattgct
      361 cgtattatat tggcagtctt aacatcaatt actattgtta tgttgcctaa aatatctaaa
      421 gcacaggcag aggggaaatc agaaaatatt ttagtttaca caaaaaaatc ttttgattac
      481 acgattatca ttgcattaat tctttttagt gtggtgttta tcaatacaaa agaatttgtt
      541 ccatggtttt ttggggagaa tttcaaaccg atgacaataa acatgcttat ttcttgtcta
      601 attatagttg ttagtccaat tggtggagtt tttgctaacc aatttgcaat tgctatagaa
      661 aaagataagg agtatgctta tccaatgatc attggtgcga ttgtaagtat tattggaaat
      721 gtagcttttg tgcctactta tggcgctgta gcggctacga tcgtactcgt ggtagtagaa
      781 ttattagtct ttttctttag agtatttttt gtacgaaaag aaataaaaat atcctatttc
      841 tttgggaaga acgttatctt attttttata gtcaatctta ctttgac
//
LOCUS       DAZUMM010000210          873 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000210 DAZUMM010000000
VERSION     DAZUMM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.227"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..159
                     /locus_tag="ACG8E6_002781"
                     /pseudo
     CDS             1..159
                     /locus_tag="ACG8E6_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          324 a          124 c          130 g          295 t
ORIGIN      
        1 cgacgaaata aattttatca aagtctccgt acagcctctt ccacgattaa gggcatggag
       61 accattcgag gaatatataa aaagaactga agaaatggaa cgctcttcgg cttttcggtg
      121 tctactgaaa tcaaggtatt aatgggaata acagcctaag atatttggag ttcagagagg
      181 gcgcgtttga ttttcaaact tcgcaacaga acccattagc aatatataaa agaaggtatc
      241 aagagtcaca gtgaatttat tccgaaaagt gaaaagagca aatataaaat ttaataaaaa
      301 taagaactaa ataggaaaac gtagacgtaa ttttctacgt tttccttttc atatatatta
      361 aatattacgg aaaattatat aaactaattt agtgttttgt ttgtaattcc taattttgct
      421 ccacttttag ataattttta ggtctattta actcattaga cattctatgc tcatttttct
      481 ccaaatttgt ttcaactctt tcgtacttaa ttttgcgtat ttgaaaattc ttcttttaga
      541 gtacttttat tacgatacta gtatgatata tatctacagc aaacagttaa aatcttccta
      601 aaataagcga atagcattcc taaaacctat aaaattttta gatgaagcga caagtggaca
      661 attaataaac tacttgaaca tgaaattatg gatcaccttt caaatttaga aaatggaatt
      721 cttatactta ttgcgcaaca tttattgatt gcaaaattat gtgacaaaaa gaactagtag
      781 aagaaggaat aatggcttga taataaagta ctacctatta aaggtagtac tttatttaat
      841 ttagtataaa ttaacatact actatatttt agg
//
LOCUS       DAZUMM010000211          826 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000211 DAZUMM010000000
VERSION     DAZUMM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.228"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>826
                     /locus_tag="ACG8E6_002782"
     CDS             <1..>826
                     /locus_tag="ACG8E6_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HFS5063104.1"
                     /translation="IENINISIRIENFYTLIFDNGKNLDEVYECIYKVVQFFYVLFGS
                     ELPVKFIEFEYGSINIGNKVIGKKYRMYVRQNAKVQSRKLRPYELFPYATIADNLSKY
                     INNWFAFYADLETIIQTYVGDLQLTSFLETQFLNACRNVEIFHRIYLEKEKIEGPEIV
                     FMRKKLMEIVTGAEEPVRKYFSERINYTGEETLSKRIKESLKVVPNPILKETILYRSK
                     SLSDSKTRFVRACVNTRNFLTHGSQDKSKYQPIFEKENLYMATKILNTIIRYFVLE"
BASE COUNT          327 a          102 c          136 g          261 t
ORIGIN      
        1 caattgaaaa tatcaatatt agtattcgta ttgaaaattt ttatacatta atttttgata
       61 atggaaagaa tctagatgaa gtatatgaat gtatatataa agtagttcaa tttttttatg
      121 tgttatttgg ttctgaactc cctgtcaaat ttatagagtt tgaatatgga tcgataaata
      181 taggtaacaa agttattgga aaaaaatata ggatgtatgt caggcagaat gctaaagttc
      241 aatcaagaaa gcttagacct tatgagctat ttccttatgc cacaatagcc gataatttat
      301 caaaatatat taataattgg ttcgcatttt atgcagattt agagactatt atacagacat
      361 atgtaggaga cttacaactt acatcctttt tagaaacaca gtttctcaat gcttgtagaa
      421 atgtagaaat ttttcatcga atatatttgg agaaagaaaa gatagaaggc cctgaaattg
      481 tgtttatgag aaaaaaactc atggaaattg taacaggggc agaagagccc gtcagaaaat
      541 acttttctga aagaataaat tatacgggag aagaaacact ctcgaaaaga ataaaagaaa
      601 gcttaaaagt tgttccgaac cctattctaa aggaaactat cttatacaga tctaagtcat
      661 tatccgattc taaaactaga tttgtgagag cgtgcgtgaa tacaagaaat ttcttaacac
      721 atggcagtca ggataaaagc aaatatcaac ccatttttga aaaagaaaat ctatatatgg
      781 caacaaaaat tttaaatact attataagat attttgtact tgaaaa
//
LOCUS       DAZUMM010000212          811 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000212 DAZUMM010000000
VERSION     DAZUMM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.230"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACG8E6_002783"
     CDS             <1..672
                     /locus_tag="ACG8E6_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS5063105.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAZUMM010000213          779 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000213 DAZUMM010000000
VERSION     DAZUMM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.232"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(190..779)
                     /locus_tag="ACG8E6_002784"
                     /pseudo
     CDS             complement(190..779)
                     /locus_tag="ACG8E6_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          250 a          150 c          147 g          232 t
ORIGIN      
        1 tcaaaaaagc gtactgtgat acattctaat gacgagagcg tctttaccgg aaaaatcaat
       61 cttttagatt caaaaaaact agtactttct ataatagatt acgaaagcat cacaaagacg
      121 aacaaaaaaa acaataaata tagatgcaga ctgtagacat catccataaa atggactttg
      181 tctacagtct gaaacgagat ctttcttctt acgtgaagga agaatatcca gtaatttccc
      241 agtatcgcca tccatacaag aaaaagaata gggaccgact tgatttttca atgaacgaaa
      301 ttcatccacc aatgaacagc gaggcaacca attccttttc acgtttgtca tggggagcag
      361 agaagcgagt gttcgcgaaa cggttgacca agaaacgccg caatgtttag aaatcgtcgt
      421 cattgcttgt ttttcagtca attcctccaa tattttataa tgtacttgtt tggcgataaa
      481 acagtttttt tcaacaatgt aggtattcgc cgtaaaatag gtgtgacagc cacgacagag
      541 gtaccgttgc tttgccaaag caagataagt ccgttgattc gcacacttat tgagaaggac
      601 cagcgatttt ttctttccgc tcttaacgat cagcccatgt tgcttcacct gtttacactg
      661 aggacagcgc tgaatcgttt taatgagcgt ggcagaaaca acgatactgt tgattgaatg
      721 tatttttttc ttctcgacat gagtgatttg aatgttttta tctttgattc ctagtaata
//
LOCUS       DAZUMM010000214          740 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000214 DAZUMM010000000
VERSION     DAZUMM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.234"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..731
                     /locus_tag="ACG8E6_002785"
     CDS             51..731
                     /locus_tag="ACG8E6_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFS5063106.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIPA"
BASE COUNT          265 a          124 c          148 g          203 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc taccacgatt aaaggcatgg aagccattcg aggattatat aagaaaaccc
      661 gaaaagaagg cactctcttc gggttttcgg tctgtactga aatcaaggta ttattgggaa
      721 tcccagctta aatcatagat
//
LOCUS       DAZUMM010000215          702 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000215 DAZUMM010000000
VERSION     DAZUMM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.235"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..699
                     /locus_tag="ACG8E6_002786"
     CDS             <1..699
                     /locus_tag="ACG8E6_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5063107.1"
                     /translation="RLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQL
                     IIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYE
                     KRVWRASFKDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDI
                     SGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK
                     LRIMIRHGKTFLTK"
BASE COUNT          227 a          143 c          130 g          202 t
ORIGIN      
        1 cgcttgcctt atttagaacg ctatttttct cggttttcat tagcgactcg tgaagccgtt
       61 caattgattg ttattgatat gtatgcgcct tatgtttcgt tggtgaaaaa gcttttcccg
      121 aatgctcagt tgatcatcga tcgtttccac atcgtccaac atattggcag aactttcttg
      181 aatcatcgag tgaaagaaac cacggtacga cttaaaggaa attcccactc tcaacgaggg
      241 ttagggaaaa aattgaagcg ctattggaaa ctcctacaaa aagaagaagc aaagttaaac
      301 tatgaaaaac gggtttggcg tgccagtttt aaagattatt tgactgaaag tgagattgcc
      361 gatcgactgt tagctgcttg tcccaactta cgccaaggct atcagttata ccaagacatt
      421 ctttatgcgg taaagaaaag agatcagtca ctatttgagg attgtttaac tagagacatt
      481 agcggtctgc cagaaaccta tacaaccact ttacgaacgt ttaaaaagtt tctgccacag
      541 atccaaaatg cgcttcacta cagctattca aacggaccat tggaatgctt aaacaaccac
      601 atcaaagtat taaaacgcaa tgcgtacggc tttcgcaact tttacaattt caaactacga
      661 attatgattc gtcatggcaa aacattttta accaaataaa ca
//
LOCUS       DAZUMM010000216          694 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000216 DAZUMM010000000
VERSION     DAZUMM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.236"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..627)
                     /locus_tag="ACG8E6_002787"
     CDS             complement(52..627)
                     /locus_tag="ACG8E6_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFS5063108.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          228 a          151 c          124 g          191 t
ORIGIN      
        1 tgaaaaaaca gagccagatt agcattattt tgctaatcca gctctgttta ttcagcctat
       61 ttttcttttt gaatatatca agcgttgggg caacatagtg atgactaatg aaacaacgac
      121 gatagacaac aagcgatcaa ggtagtctgt acccgcctgt accaagatca cacttgttgt
      181 ctgacttaaa cctaacccat gtaatagctg aacgatcata ctggatcctg aggaggtgat
      241 cccgccaaat aactgaacgg taatgatcga agaaacgatc gttccaggta ccgacaaaat
      301 caaagcgagt aataacagtt tcaagctgtt tttatctttg ccgagatatc ttggcaaaat
      361 caatccagca agcaagcctg tgagaatttg gattggcgaa taatacagag aaaaaatatc
      421 tgtagtcact ccacttaaaa taccgctgga caatcctgtc agcattccag gtacaggccc
      481 aagcaatgca ccagcaaaaa tcgttccgat agaatcgaga tagattggca aacgaagcca
      541 taaagcaatc gttcctccaa tataattgat cccaatacac aaagccatca ctgtaagtac
      601 gcgagttgcc attttgtttt tattcatttt gttagcccat ctttaacctt tccaaatctt
      661 tcataatcgt ttcttcttgt gcatttaaaa gaac
//
LOCUS       DAZUMM010000217          677 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000217 DAZUMM010000000
VERSION     DAZUMM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.237"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>677)
                     /locus_tag="ACG8E6_002788"
     CDS             complement(<1..>677)
                     /locus_tag="ACG8E6_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS5063109.1"
                     /translation="LLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANLYA
                     EFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFSID
                     QEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCLQK
                     IQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLLEQ
                     LAQIFFI"
BASE COUNT          239 a           70 c          130 g          238 t
ORIGIN      
        1 tgataaaaaa aatttgggct aattgttcaa gcaaaaaatt ttctgaaaga tgttcttcta
       61 aatatcgtat gattggatca actaattcat ctgaataaag aatatgcttt tttttaggaa
      121 tgactttctc atttgaaggt attgtcaata aagcaagttc ctgttgataa tgccttggta
      181 aggaagacaa ttgaattttt tgcagacaat aagtaactaa taaggaaagc aaatgaatgc
      241 tcatctgttg gcatagtatg aaaaaagaat tgatttcctg atcataggaa atagctggct
      301 tactatgttt tagataatca attttgaact gttcattttt cagaaaagta atgatagatg
      361 ttatttcttg atctatagaa aaagattgtc ccgtaatatg tgctaataat tgctcatcaa
      421 aataaaactg gataatgata aaagtttgtg tttccttttg agtagaaaag atttggactt
      481 cttgctgttt atcaatgaat aaaagggaag gattatctag ttctatagat ttatgtgcaa
      541 attcagcata taaattagcg ctttcattta ttagtagtcg aaaataggga gcagcagata
      601 aatgaagatg ggtatctatg gaataggtta gtgttttttt gacaagattg gaggattggt
      661 tgtttgttaa caaaaga
//
LOCUS       DAZUMM010000218          611 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000218 DAZUMM010000000
VERSION     DAZUMM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.239"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..503
                     /locus_tag="ACG8E6_002789"
     CDS             <1..503
                     /locus_tag="ACG8E6_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5063110.1"
                     /translation="RNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQS
                     PKSILRGSFNPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNY
                     TNKEDYLRSINILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLI
                     KIESVR"
BASE COUNT          237 a           76 c           90 g          208 t
ORIGIN      
        1 atagaaattt atttgataat ataagattcc cactgggtaa attaatggaa gataaggcta
       61 ctacttattt gctgatcgat cattccgaaa aagttgctgt caattcatct gctaaatatc
      121 actattacca aagccctaaa agtatactga gaggaagctt caatcctaga atttttgatt
      181 cttttgaagt ccatgaagat atactaatat tcatagataa gttttatccg gaattatcaa
      241 ttaatgttag aactcgctat gtctatgaag caataaggat gatgatgaca atgattaaaa
      301 ataactatac caataaagaa gattatttac gttctataaa tattttacaa cgctttgaaa
      361 aagaagtgaa aattgatccc gacttttcac caaaattaaa aaaattagta aagtttttat
      421 taataaataa aaacttgcct tactatatgt caagtagtaa gatagcaagt aaattcttga
      481 ttaagataga atctgtgagg taagagatga agaatataaa aaaatttttt tcaaggaata
      541 ttttcccggt ttttattaaa atattaccta ttcaaaaaaa tttaattgta ttttcaagtt
      601 ttggcgggcg t
//
LOCUS       DAZUMM010000219          563 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000219 DAZUMM010000000
VERSION     DAZUMM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.241"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(115..537)
                     /locus_tag="ACG8E6_002790"
     CDS             complement(115..537)
                     /locus_tag="ACG8E6_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063111.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
BASE COUNT          179 a           98 c           83 g          203 t
ORIGIN      
        1 taaagttcgc tgaacttgta aagctgattc ttctttcggt aaattaattt tacttagaat
       61 atcgctatct tgataaaaat ttggtgtgtt cacaaattct ggcataaaaa atccctactt
      121 tctagttaag gttcctgaat aagtcgcaat ccaattgttt ccagctgatt tataactttg
      181 taaatacaat tttccatagt accaataacc atttcttttt tctttccaat tccgtgattg
      241 aggaggtcta gaaggccaac caactttatc aaaaatcatc gttcctgtca ctcgtatcgt
      301 tttagaagct gctcgagcaa gtaatggaga aactattaaa taattttcag taacagttaa
      361 gtcgttagtt gggttaccaa tggctattcc gttcctatct aaaggagtta attcttggtt
      421 attatctact acagtagttg gttcatttgt actatcagca aatgtaggaa ttgtagttcc
      481 agttacacta attccaatag ttaaaatacc taaagataaa ataaacttat ttttcattgt
      541 ctatcacctc gaaatcaaaa ata
//
LOCUS       DAZUMM010000220          548 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000220 DAZUMM010000000
VERSION     DAZUMM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.242"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>548)
                     /locus_tag="ACG8E6_002791"
     CDS             complement(<1..>548)
                     /locus_tag="ACG8E6_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS5063112.1"
                     /translation="SLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW
                     ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPT
                     IFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG
                     NDDLLEKYMSGKSLEALELEQE"
BASE COUNT          147 a          119 c           84 g          198 t
ORIGIN      
        1 tcctcttgtt cgagttccaa tgcttctaat gatttaccgg acatatattt ctctaaaagg
       61 tcatcgtttc cctctattac cgtatcccat tgttcagatt cggtaaagtt cgtcacacac
      121 atattaggat acagttctac cttctgtttg attacaattt cggcagaaag tttctcttta
      181 atatcctgat aaaccgttga taaatcaatt ccattttggt caatcttatt gataaaaaag
      241 attgtgggaa tccccatttt cctaagtgca tgaaataata tacgagtttg tgcttgtacg
      301 ccatcttttg cagaaatcag tagaattgcc ccatctaaaa ctgataatga acgatatact
      361 tctgctaaga aatccatatg tcctggcgtg tctatgatgt tcaccttcgt attttcccac
      421 tgaaaagagg ttattcctgt ctgaattgta attcctctct gacgttctaa aagcgtatta
      481 tccgtcctcg ttgtaccttt gtccacgctt cctaattctg taatcgctcc actgttatat
      541 aataagct
//
LOCUS       DAZUMM010000221          547 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000221 DAZUMM010000000
VERSION     DAZUMM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.243"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(148..519)
                     /locus_tag="ACG8E6_002792"
     CDS             complement(148..519)
                     /locus_tag="ACG8E6_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063113.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          169 a           97 c           74 g          207 t
ORIGIN      
        1 tgtatacttt ttgtttgata tcgtgactga cgtgccggac gcacgtagcg atcatcggcg
       61 ttatgactat ataaagtata ctttcgttga tccagaaaga tacaaagatt aaataaaaaa
      121 ataacaaaca aactcgcttg aaattcatta ggtgagtttg tttattattt tttctgcttc
      181 tcgattcatc agcgccatat ctgttttcgt aatggtttca ttaggaatat agtcagcaca
      241 ttttcttttc tctaacattc tatacccact attatgtatt cctaaggtat tcaattcctt
      301 aactgttgaa aacaaattcc aaacggtttg gtggcttcct tttccgctac ttccattata
      361 aataatatca taattttctt tcaaatactc atcagatttt ttaaaagcac tgtagtatgc
      421 tcttgaaaca gcagttcttc tataagattc tttagaatta tatttatcat ctgattcaat
      481 ttgtttggaa atagataaat attcattaaa gtcaaacatt ttattccccc aaagttgcta
      541 ctacaga
//
LOCUS       DAZUMM010000222          532 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000222 DAZUMM010000000
VERSION     DAZUMM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.244"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>532
                     /locus_tag="ACG8E6_002793"
     CDS             <1..>532
                     /locus_tag="ACG8E6_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5063114.1"
                     /translation="KQNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYT
                     GLSSDFSENMVFVDYSTSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDE
                     SKYFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGI
                     FLSIGTSKIQIIMQFIF"
BASE COUNT          213 a           47 c           93 g          179 t
ORIGIN      
        1 aaacaaaaca atctaaaatt aggtgatgaa gttgatcttg aattactaga tattgaaaaa
       61 agtggaaaaa taaaaagtca taaatttaaa attataggga tcttttctgg taaaaaacag
      121 gaaacatata caggattatc ttctgatttt agtgaaaata tggtgtttgt agattattca
      181 acaagccaag agatattaaa caattcagaa aataatgaaa ttgcaaataa aattttaatg
      241 tattctggta gtgcagaatc tacagactta gccttaaaca aattgaaaga gcttaaaatt
      301 gatgagtcaa agtattttgt tgaaaaagat tctaatgcgt ttgaagagtc tttagagtca
      361 gtgagtggaa taaaatatat aataaaagta atgacttatt ctattatgtt gggtgggatg
      421 gttgttcttt cattaatctt gattctatgg ttaagggaaa gaatttatga aataggtata
      481 ttcttatcta ttggaacatc taagatacaa attataatgc aatttatatt cg
//
LOCUS       DAZUMM010000223          524 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000223 DAZUMM010000000
VERSION     DAZUMM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.245"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          233 a           73 c           85 g          133 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactctc gatgaagtag acct
//
LOCUS       DAZUMM010000224          522 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000224 DAZUMM010000000
VERSION     DAZUMM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.246"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..412
                     /locus_tag="ACG8E6_002794"
     CDS             152..412
                     /locus_tag="ACG8E6_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063115.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          179 a           61 c           71 g          211 t
ORIGIN      
        1 aagtcctctt ttctgtgaat tggttgtcgt taactttatt ctacagaagc gacttccttt
       61 tttgtatgga ttttttcatt tacacaaaat attttacact ctcaattatt ttaatataag
      121 tacttcagat ttaataaagg agaaatatct aatgaaaaaa attgatttaa taaatatgat
      181 aggtatgtta atagggatat tagtaaatat agtaattttc acagattggc tcggggtgtt
      241 attttctaat cttattccca tacttattat aggcatttgt ggaattatac tttcaatatt
      301 agaacttttt gaaagcagaa atacaatgaa tagaatattt gcttgtatta tattaattgt
      361 taatctattg cctatggtct attttacttt tttatatttt gcactgggat gaaataaatt
      421 aaccaaaagt ttctttttct tataatgtgt atcaatatta aaactgaaaa agatatggat
      481 aataggatca aagccctcat tggaacactc aatatattat ca
//
LOCUS       DAZUMM010000225          514 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000225 DAZUMM010000000
VERSION     DAZUMM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.247"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG8E6_002795"
     CDS             complement(<1..132)
                     /locus_tag="ACG8E6_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS5063116.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAZUMM010000226          500 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000226 DAZUMM010000000
VERSION     DAZUMM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.248"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>500
                     /locus_tag="ACG8E6_002796"
     CDS             <1..>500
                     /locus_tag="ACG8E6_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
                     /protein_id="HFS5063117.1"
                     /translation="SLYPVYHGSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFK
                     IEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEI
                     VILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDS
                     DPLLQY"
BASE COUNT          179 a           82 c          100 g          139 t
ORIGIN      
        1 tccttgtacc ctgtttatca tggaagcgca aaaagcaaca tagggattga gcagcttata
       61 gaagtgataa cgaataaatt ttattcatca acatacagaa agaagtctga actttgcgga
      121 aatgtcttca aaattgaata ttcggaagaa agacaacgtc ttgcatatgt acgcctttat
      181 ggcggaatcc tgcatttgcg ggattcggtt agaatatcgg aaaaggaaaa aataaaaatt
      241 acagaaatgt atacttcaat aaatggtgaa ttatgtaaaa ttgataaggc ttattccggg
      301 gaaattgtta ttttgcaaaa tgagtttttg aagctaaata gtgttcttgg agatacaaag
      361 ctattgccac agagagagag aattgaaaat ccgctccctc tgctgcaaac aactgttgaa
      421 ccgagcaaac ctcaacaaag ggaaatgtta cttgatgcac ttttagaaat ctccgacagt
      481 gacccgcttc tacaatatta
//
LOCUS       DAZUMM010000227          494 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000227 DAZUMM010000000
VERSION     DAZUMM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.249"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..160)
                     /locus_tag="ACG8E6_002797"
     CDS             complement(<1..160)
                     /locus_tag="ACG8E6_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5063118.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNS"
     gene            complement(179..340)
                     /locus_tag="ACG8E6_002798"
     CDS             complement(179..340)
                     /locus_tag="ACG8E6_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFS5063119.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT          169 a           96 c           58 g          171 t
ORIGIN      
        1 aagaattaga actttcatat aaagcctttt ctatttcatt gcttgatttc attattgtta
       61 aacaagaata caagcaagaa agaactatag ttaatatgat aaaaattatt aaagttctat
      121 ttttctttct tgtaatatat gctatagcat tttttatcac tttcctaact actcctttct
      181 attttcttac tccataacta attgcatcca ctggacaagc atttttacat cgtccacatc
      241 tgatacactc cagatgattg caattctcaa ctgaatcttt attcatttgg caaacttttt
      301 tacattttcc acaattaata cacttatcct tatcctgaat cggctacact agactagaca
      361 gaaaaaataa ggtgtgtaga ataataaaaa acacactgga ggatttttca tgtcaagacg
      421 tcaacgaaga acctattcaa aagaattcaa acaacaaatc gtcgatctct atctcgctgg
      481 taagccttgc gcag
//
LOCUS       DAZUMM010000228          492 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000228 DAZUMM010000000
VERSION     DAZUMM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.250"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>492
                     /locus_tag="ACG8E6_002799"
     CDS             <1..>492
                     /locus_tag="ACG8E6_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFS5063120.1"
                     /translation="DFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDYEK
                     YDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTI
                     IHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFM
                     DAT"
BASE COUNT          178 a           89 c           81 g          144 t
ORIGIN      
        1 tgattttact acagaaattg tgcaaactct agtcactaaa ggcgatttaa atgaattatt
       61 ccgttcgcac ttagaaaaag cgataaacac actcctacgg actgaattaa cggctttttt
      121 agattacgaa aaatatgatc gcactggttt taattcaggt aattcgagaa acggttctta
      181 ctttcgatca atcaaaaccg aatatggtga attaacattg gaaataccta gagatcgtaa
      241 tggtgagttt aaacaacaaa ctttaccagc ctacaaaaga acaaacgata cattggaaac
      301 cactattatc catttattcg aaaaaggtgt tacgatgtct gaaattgctg atttgatcga
      361 aaaaatgtac ggtcatcact atactccaca aaccatgtcc aacatgacta aagttctgac
      421 tgaagaagta aatgccttta aatccagagc cttaaatgat aagtatgtcg ctatttttat
      481 ggacgctact ta
//
LOCUS       DAZUMM010000229          482 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000229 DAZUMM010000000
VERSION     DAZUMM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.251"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..143)
                     /locus_tag="ACG8E6_002800"
     CDS             complement(<1..143)
                     /locus_tag="ACG8E6_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS5063121.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQ"
     gene            complement(154..360)
                     /locus_tag="ACG8E6_002801"
     CDS             complement(154..360)
                     /locus_tag="ACG8E6_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFS5063122.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          137 a          107 c           66 g          172 t
ORIGIN      
        1 tactgattat ttgctacttg tgagctacct tcatttgcgc ccaaattaaa ttcattgttg
       61 atagtatagg caaatccgtt aattggcaat aaaaaaagag ccgttaacag gctcatcgca
      121 gtaatagtaa ttttcttttt catatctatc ctcctattta tctgatgaaa ttttcttata
      181 cgttcgattt gatacaccta aaatactccc tagaaatgct ccaaaacctg taataatcat
      241 caccgttata tctgtgtacc gccagttaac accttttccg acaagaccga caaaagtcgc
      301 taaagcgggg ataactacta tagcgatcca tttcaaaata tcaaaggttc tattgttcat
      361 actatccacc tcccttcatt ggttgggcta ctttcttttt caatcgcttg ttttcttcta
      421 tcaatatttc tctttctgaa agcaaggcat caatttgccc gtccagttgt tcaattttat
      481 ct
//
LOCUS       DAZUMM010000230          475 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000230 DAZUMM010000000
VERSION     DAZUMM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.252"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..210)
                     /locus_tag="ACG8E6_002802"
     CDS             complement(<1..210)
                     /locus_tag="ACG8E6_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5063123.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
BASE COUNT          146 a           87 c           57 g          185 t
ORIGIN      
        1 taaatcagat acataagccc atgtacccgt tcgttgtaaa taaagttctt cttgtaacaa
       61 ctcccatttt ctcttagaaa tcataatgac gccatcgttc aatccatttt tagaatccct
      121 agtaatttct atttcagaat ttgaatgaac tacttcttgc aacactttac ctatattttc
      181 tttgaagttt gttggagtaa tagtatccaa aatgaatacc tctcttctct atacctctat
      241 tatgaagtgc ccataaaata aagcaagcgt tgaggaataa ttctatcaac acttgcttta
      301 ttaagctttc attttgtagt ttgactaaaa ccaataatct tatttctaaa ctctcgaaaa
      361 acttcttgtg gttgataata gtctggatct atttcaaatg tttgtgagaa taattcattg
      421 aatttttcta ctgaaataat gattttacca ttcttctctc gactgttttc tatta
//
LOCUS       DAZUMM010000231          444 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000231 DAZUMM010000000
VERSION     DAZUMM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.254"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..379
                     /locus_tag="ACG8E6_002803"
     CDS             <1..379
                     /locus_tag="ACG8E6_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5063124.1"
                     /translation="HRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTE
                     GFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIA
                     FGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT          141 a           93 c           80 g          130 t
ORIGIN      
        1 tcatcgactg tttaaacgac caattagtca aacagaaatc gttgatgagc tgttaagcta
       61 caacgaagaa ttgcgaactg cgtatcattt ttgtcaatta ttacgctatt atttcgttaa
      121 acgaggtacg gaaggctttc aagaaaccct taaaacaatt tcatctacgt tacctcagtc
      181 gtttaaaaag aaattcacga tcttccatcg ctaccaatcg ggtatttcaa atgcctttaa
      241 ggtgtcccac tcaaacggcg tcactgaggg gctgaataac aagatcaagc tgattaaacg
      301 aattgcgttt ggttatcgca acttttataa ctttcgggca cggatctatt tacaacaagg
      361 tctgatcttt gaaaactaaa aaaatatgcg gcgcacagaa cgcgccgcat atcggactaa
      421 gttttattca aatttgtctt cacc
//
LOCUS       DAZUMM010000232          440 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000232 DAZUMM010000000
VERSION     DAZUMM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.255"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            79..>440
                     /locus_tag="ACG8E6_002804"
     CDS             79..>440
                     /locus_tag="ACG8E6_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5063125.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKK"
BASE COUNT          163 a           55 c           95 g          127 t
ORIGIN      
        1 acttcggaag actaaaattg ttactggtaa tgatacattt gtaaaaataa aaaagatgat
       61 ttttaggaag tgaagagagt gcataaaatt agtgttatag tacccgcata taacgtagaa
      121 gactatttag aaaactgctt agaatctatt tccaaccaga cacattcaaa tattgaaata
      181 atagtagtca atgatggttc cccggatcga agtggggaaa ttgcagaaga atataaaaag
      241 aaagatatgc gattaaaagt gatacatcaa gtgaatggtg gtttaagtgc ggctaggaat
      301 tctggtcttg attctgcaac aggagatttt gtgtgcttta ttgacagcga tgattggata
      361 gaaccagact atcttgagtt actatatttt ggcacgcaaa cttatggagc agatatctcg
      421 gtgctaaaaa taaaaaagat
//
LOCUS       DAZUMM010000233          440 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000233 DAZUMM010000000
VERSION     DAZUMM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.256"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            240..440
                     /locus_tag="ACG8E6_002805"
                     /pseudo
     CDS             240..440
                     /locus_tag="ACG8E6_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          152 a           74 c           66 g          148 t
ORIGIN      
        1 tcttttcata aatattgtaa atagaagtat taaaaatata tacaaaaagt attatttcga
       61 gaaagataaa tattttcaaa aaccttttca taatcctcac cctttcttac actgataaat
      121 catgcgtcta atattcaaat tatactcgat atgctttagc taatcagcca actctgtttg
      181 tctactatca ttatatcaat agacaatatc tgctcccgtc tttatgatcg tgccaaagca
      241 atcaaaagaa aattgcgaat cacttagatc gaaagtttac tcaaacaaaa gcattgtcag
      301 cggttgtcac ggatttaact tatgttcggg tcgggacaaa ttgggcttat atttgtttga
      361 tgattgattt attcaatcgt gaaattattg gccattcttg cggaaataaa aaagatgcgc
      421 agcttgtaaa aagggccatt
//
LOCUS       DAZUMM010000234          408 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000234 DAZUMM010000000
VERSION     DAZUMM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.258"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>408)
                     /locus_tag="ACG8E6_002806"
     CDS             complement(<1..>408)
                     /locus_tag="ACG8E6_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFS5063126.1"
                     /translation="GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAP
                     QEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLA
                     ELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
BASE COUNT          130 a           77 c           70 g          131 t
ORIGIN      
        1 agttatttta ttgaacatat atcttacttt atctatccga ctatttagac gacgggtctg
       61 gcaaacaggt tcgccagtgg taacctgata tccttttagc tctgctaaac aaacactaag
      121 cccatttgta aaaaaagtta aatcattgcg ataatcttga atacatcgag caggaatttc
      181 tccaataata atgacctcat tatttttcag ttgagtattt acgatatttg cacaatattt
      241 gggagcatcg ttatatgccc gtgaaagata ttcctgtggt gcataaactt taaaactaag
      301 atatggctct aacaattctg ttccagcttt tctaaaggct tgctccagta caataggagt
      361 aagcatccga aaatctgctg gagtactaac agggctatag tataaacc
//
LOCUS       DAZUMM010000235          396 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000235 DAZUMM010000000
VERSION     DAZUMM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.260"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..202
                     /gene="tnpB"
                     /locus_tag="ACG8E6_002807"
     CDS             <1..202
                     /gene="tnpB"
                     /locus_tag="ACG8E6_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS5063127.1"
                     /translation="QDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVR
                     WLLEGLSIDQPRAILPGKKGVF"
BASE COUNT          130 a           73 c           83 g          110 t
ORIGIN      
        1 tcaagaccgt ttcaaacttc tctattggga cggtgatggt tttcttctct gttataagcg
       61 gatagaaaac ggcaagttaa aatggcctcg gacgaaagac gaagttcgaa ccttaacgaa
      121 ccaacaggtt cgatggttgt tagaaggatt aagtatcgat caacctcgag ctattttgcc
      181 aggaaaaaaa ggagtttttt aagctggggg gttaacacaa aaaatgtgtt gaacccctat
      241 ttcttgtgcg tttcttgtgg tattctgtaa caaaatcagt tcgattcaag gtggtgaaaa
      301 attttggaaa ctacagatac gttgcttcag ttgcttcaag aagcccataa aaccaatcaa
      361 gcacaacaac aaaccattca gaatcttaca actgaa
//
LOCUS       DAZUMM010000236          370 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000236 DAZUMM010000000
VERSION     DAZUMM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.262"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..251
                     /locus_tag="ACG8E6_002808"
     CDS             57..251
                     /locus_tag="ACG8E6_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063128.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            241..>370
                     /gene="tnpB"
                     /locus_tag="ACG8E6_002809"
     CDS             241..>370
                     /gene="tnpB"
                     /locus_tag="ACG8E6_002809"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS5063129.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDA"
BASE COUNT          128 a           62 c           73 g          107 t
ORIGIN      
        1 actaacaaat gcacctcaag atatacttga gctatcaaga agtctggagg ttttctatgt
       61 ataaattaaa tgagattgtt cctgtccaat taggaaacga aaagagtgca ccagaaaaag
      121 gtaaacccgc ggctgccaag ttagcgatgc gcatcaaaag agagaatggt tctgaagttt
      181 tgatctataa cggtattaac aattatattc ttcaagctat tttgaaagaa ctgttgtcca
      241 atgctttatg atttacttca aaccaaacat atttatattg tgtgcggaaa aacagatttg
      301 cgaaaaggaa ttgatggtct tgccagtctt attcaacaag agtatcaact cgagctttac
      361 gaagatgccg
//
LOCUS       DAZUMM010000237          368 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000237 DAZUMM010000000
VERSION     DAZUMM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.263"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>368
                     /locus_tag="ACG8E6_002810"
     CDS             82..>368
                     /locus_tag="ACG8E6_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFS5063130.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCR"
BASE COUNT          130 a           59 c           76 g          103 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcg
//
LOCUS       DAZUMM010000238          359 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000238 DAZUMM010000000
VERSION     DAZUMM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.264"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..272
                     /locus_tag="ACG8E6_002811"
     CDS             48..272
                     /locus_tag="ACG8E6_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFS5063131.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT          181 a           39 c           73 g           66 t
ORIGIN      
        1 aaaaattatt ttgcgtacat atgattaaag aaaggagagt gtgacacatg gaaacagaat
       61 ggcaaagacg tgaaagaaaa aaacagaaaa aagaagagat gttacgatta tgtcgattat
      121 tagatgtaaa agactgggac aaaaacgagc aaattgcaca aaaagtacga tcaatcatcc
      181 ataaagagga aaaagttaaa aataaagaca cgcgtagaac tgaaaatgaa aagagaaaag
      241 tacaaaagaa aagagaaaaa tcaaaaagct agaaagaaaa agaggctgag acagaagcgt
      301 ttaactccaa gaaataagct ggaattcaca aaaatttgaa aagcaatttt cgtgaattt
//
LOCUS       DAZUMM010000239          332 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000239 DAZUMM010000000
VERSION     DAZUMM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.265"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>332
                     /locus_tag="ACG8E6_002812"
     CDS             <1..>332
                     /locus_tag="ACG8E6_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063132.1"
                     /translation="IILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAE
                     YTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQ
                     GLYGWNVT"
BASE COUNT          103 a           53 c           78 g           98 t
ORIGIN      
        1 attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa
       61 aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta
      121 aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt
      181 ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct
      241 cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc
      301 gaacaaggat tatatggttg gaatgtgacg ga
//
LOCUS       DAZUMM010000240          321 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000240 DAZUMM010000000
VERSION     DAZUMM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.266"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>321
                     /locus_tag="ACG8E6_002813"
     CDS             <1..>321
                     /locus_tag="ACG8E6_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5063133.1"
                     /translation="KNYPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFM
                     IQSGESEETWTTFFEYLKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLR
                     NIFT"
BASE COUNT          117 a           62 c           60 g           82 t
ORIGIN      
        1 aaaaaaatta tccttactta atgaccgatg tactctatat aaaagtacga gaagaaaatc
       61 gagtactctc aaaaagctgt catatagcga ttggaataac caaagatggc gaccgtgaaa
      121 ttatcggctt catgattcaa agtggcgaaa gcgaagagac ctggacaaca ttttttgaat
      181 acctaaaaga acgcggttta caaggtacgg aactcgttat ttctgatgcg cacaaaggat
      241 tagtctctgc cattagaaaa tccttcacca acgtaagttg gcaaagatgc caagttcact
      301 tcctaagaaa tatctttacc a
//
LOCUS       DAZUMM010000241          292 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000241 DAZUMM010000000
VERSION     DAZUMM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.268"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>292
                     /locus_tag="ACG8E6_002814"
     CDS             <1..>292
                     /locus_tag="ACG8E6_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5063134.1"
                     /translation="RTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNIS
                     TSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHA"
BASE COUNT           84 a           61 c           58 g           89 t
ORIGIN      
        1 aaagaacatt cgtagcggat acctctgttg ctgaaaaaca ctgtttcatt agtcaaaaag
       61 ttcgttggtc tgtcattgct cgcttaaaag agaatacttc catgacggaa atcgctcgtc
      121 agaaaaatat ttccacttct tcggtctatc gtgtcatgaa gcgattctac cgcccattga
      181 atcctttcaa acagactttg ccaaaggtct tatgctttga tgaattcaaa tcggttcgaa
      241 gtgttgccag tgcaatgagt tttatcatga tggacggaca aagccatgca tt
//
LOCUS       DAZUMM010000242          286 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000242 DAZUMM010000000
VERSION     DAZUMM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.270"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>286
                     /locus_tag="ACG8E6_002815"
     CDS             <1..>286
                     /locus_tag="ACG8E6_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFS5063135.1"
                     /translation="YFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIVTDLTYVRVGKKW
                     NYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFHTDRGK"
BASE COUNT           99 a           48 c           55 g           84 t
ORIGIN      
        1 tattttaaag tacatcattc tacttgcaat gaagccaaaa cgacaaacgt attgaatcgt
       61 aaattcttaa gagacaaccc attagaagcg atcgtaacag acttgactta tgtacgagtc
      121 gggaaaaaat ggaattatgt ctgtttcatt ttggatctgt tcaatcgaga aattctcggc
      181 tattcttgtg gagaacataa agatgccgtt ctagtaaaaa aagcatttag ccgtatcaaa
      241 caacctctga cagaggttga gatttttcat actgatcgtg gaaaag
//
LOCUS       DAZUMM010000243          254 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000243 DAZUMM010000000
VERSION     DAZUMM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.271"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..81)
                     /locus_tag="ACG8E6_002816"
                     /pseudo
     CDS             complement(1..81)
                     /locus_tag="ACG8E6_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
BASE COUNT           75 a           50 c           23 g          106 t
ORIGIN      
        1 taaaaaaatt gtatctccag gatgcaaccc attttcttta agaaattcct tttaaattgt
       61 taatactact gagtgcccca cttctctgaa ttttctttaa cttctccctc ccctttttta
      121 tttgtatata cattcatata cgcttgttaa tatcaaatca accgtagtct ctcttatgtc
      181 taattatata gaaagattgt tctttctata taatttagac aatttaaaaa attaagattg
      241 cctccttctg tttc
//
LOCUS       DAZUMM010000244          241 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000244 DAZUMM010000000
VERSION     DAZUMM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.denovo.272"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..220)
                     /locus_tag="ACG8E6_002817"
     CDS             complement(<1..220)
                     /locus_tag="ACG8E6_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5063136.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFP"
BASE COUNT           63 a           45 c           46 g           87 t
ORIGIN      
        1 caggaaaaat gatttcggtt ctcccacaac gtcgcttatt ttctttgtat tgttcaaagt
       61 attcaagtgc tgttccacca catttgagaa agttatagac attgtaaatt gtttggcgtc
      121 cacgtttaag ctgcttcgca acgatagcaa ccggagtttc ttgatgaaaa tatgcttcta
      181 tcattacaag ctcgtttggt gtaagatggg tataagtcat ttatgttcac tctccttgta
      241 t
//
LOCUS       DAZUMM010000245        13952 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.guided.066, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000245 DAZUMM010000000
VERSION     DAZUMM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.guided.066"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            113..1009
                     /locus_tag="ACG8E6_002818"
     CDS             113..1009
                     /locus_tag="ACG8E6_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS5063137.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFNMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLAYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPIPSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1159..2100)
                     /locus_tag="ACG8E6_002819"
     CDS             complement(1159..2100)
                     /locus_tag="ACG8E6_002819"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS5063138.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFRSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKTAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2225..3346
                     /locus_tag="ACG8E6_002820"
     CDS             2225..3346
                     /locus_tag="ACG8E6_002820"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS5063139.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPHGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3604..5106
                     /gene="eat(A)"
                     /locus_tag="ACG8E6_002821"
     CDS             3604..5106
                     /gene="eat(A)"
                     /locus_tag="ACG8E6_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS5063140.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKSQPKHLSISYVRQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5173..5700
                     /locus_tag="ACG8E6_002822"
     CDS             5173..5700
                     /locus_tag="ACG8E6_002822"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5063141.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5911..6972
                     /locus_tag="ACG8E6_002823"
     CDS             5911..6972
                     /locus_tag="ACG8E6_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS5063142.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6975..7871
                     /gene="murQ"
                     /locus_tag="ACG8E6_002824"
     CDS             6975..7871
                     /gene="murQ"
                     /locus_tag="ACG8E6_002824"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS5063143.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7885..9867
                     /locus_tag="ACG8E6_002825"
     CDS             7885..9867
                     /locus_tag="ACG8E6_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS5063144.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSAATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9932..10378)
                     /gene="nrdI"
                     /locus_tag="ACG8E6_002826"
     CDS             complement(9932..10378)
                     /gene="nrdI"
                     /locus_tag="ACG8E6_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS5063145.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10589..12013
                     /locus_tag="ACG8E6_002827"
     CDS             10589..12013
                     /locus_tag="ACG8E6_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFS5063146.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12194..12967)
                     /locus_tag="ACG8E6_002828"
     CDS             complement(12194..12967)
                     /locus_tag="ACG8E6_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5063147.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12964..13869)
                     /locus_tag="ACG8E6_002829"
     CDS             complement(12964..13869)
                     /locus_tag="ACG8E6_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5063148.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
BASE COUNT         4421 a         2478 c         3000 g         4053 t
ORIGIN      
        1 ttttcataca aatgttaaat tttcatacaa atataggaag cgttttactg gctttttact
       61 atttaaacag gctatcatag gaaaaagtga cagagaaagt gaggcggaaa agatgaacaa
      121 aaatgacgta ctgctagttg caaaaaataa atcactttat tttttgcctt ccatccgctc
      181 tttagatgtt tggtccggaa aagatttact acagtcggaa ttcattaaat cagctagaca
      241 acagttggca aattatcagc tgatcgtttt tttagattat ggatttaata tggagatggc
      301 tcgacgcgtg cgcccattta ccaatgcaaa aattgtgtta tttttttgga atcacttcaa
      361 agcagaacac gaggcgatgc tgaagcaggc acaagatgaa ccggcaatcg atgagattta
      421 tcattttgat tttttggaag caaaagaact tgggctgaaa cataatagtt cattttattc
      481 gaaacatatg gatttttctt ctagcgaacc aaagacggat ctattttttg gtgcaacgaa
      541 taatgggaga aaagaacgag cagaaagcta taagcaggaa tttataaaaa gagatgttac
      601 gaccaactat ttcattcttc cacatcgagg gaatgaacaa cctggatatc ttgcttatga
      661 agagtacctg aaaaaaacag gggaatcgcg cgggattctg gaactgttgc gggagggtca
      721 gcaaggagtg actttgcgga catttgaatc tatgtatttt caaaagaaac tagtgacgga
      781 taatcaagcg attgctcatt atcattttta tcgtcctgaa aatatttttt tgcttcgaga
      841 acgtgattta gatgaattac cggaatttct gcatacacct tttaagccaa tcccatcaga
      901 gctattggaa ttttatgatg cagaaaattg ggcaaaacga tttatcacta acgattccac
      961 tatttatgag caatacgaat atgacctctc ctctgttgaa aataaataaa aaacctacat
     1021 tttgaagaga ttatgtaccg aaacaaacaa gcacaattct tatggcatca tgagagttgt
     1081 gttttctttt tgatctttca ttgggcttaa aaaaagagaa ccagctgctg attcagctga
     1141 ttctcatcag agagcctttt attctgcgcc aaggattcct tcttttgcat ggactaattg
     1201 ggtcaaaaag gcacaatatg gtgtagcaat atcatatttt tgtccttttc gccaaacggc
     1261 accattgatg tagtcgattt cagtcaaacg atggtttttg atcaggtctt ggtacataga
     1321 tgggtaatgc aacccgatgt tgtcaggatc ataagtagaa gcaatatgtt ggtacacttc
     1381 ttcttgatct aatatgattc cttcttttgc tgcgatagaa gcaaattcag atacgattgt
     1441 tcgaaccatg ctctctgccg ttttttgcgc gcctagttca gcaatattac aatccaaaat
     1501 cgtgcataaa ccattcaatg ttccatttac acaagctttt cgccaaatgg aataacgaac
     1561 attatggcta tagatcggat ttaattttgc ttcttgaaag acttccacaa ctttttttgt
     1621 aaatgcttcg ccttctggtt ccaagttctc tagttcgatt tctccatcac ctaacaatgt
     1681 gacttttccg ggaccagcta gaccagctgt ccacatcgtg atcccaaata agatattttt
     1741 ctttggcaca tatttttcca gtacatcttc atgacctaat ccatttagta aacacaaaac
     1801 ataagtattg tcagtaataa tggattggat cgaacgaaac atatcatcta attgattcgc
     1861 ttttgttaat gctacgatca agtccacgtg ttcgttgctt tcgatgattt cttctggtga
     1921 atagattggc agttttgcga ccacttcttt accgttaaag tcagcaatca acccatttgt
     1981 acgaatggct tcaatgtgtg caggccaacg atcgatcaag aggacctcat ttccgctttg
     2041 atgtagcatc aatccgattc ggctgcccat tgctccggct ccagctattg cgattttcat
     2101 gttttttctc tcctttatca atgagttccc ttttagtata tagaaaaatg gaagaaaata
     2161 gcattgtttt gtttgaaaaa aatgaaaaaa agtgtcttac ttaagaagaa agtgagggat
     2221 acagatggat attttggcgg caattgattc atttaaagga agtgcttctt catttgaact
     2281 aaatcaggca gttctaaatg gctgggaaga tcgtaaagga agaaaaatca atgtgccgat
     2341 tgcagatggc ggagaaaata ctgcagaagc aatctattac gcgctcgggg gaacatggcg
     2401 caccattgat gggatggatc ttttattccg tcagcgaaaa atcagctatt tattgacaac
     2461 ttggcaagga aaaaataccg cagtcatcga atctgcacaa gcattggggt tagatctact
     2521 tgataaacca acggatcaaa ctatccgact tgcttccagt tatgccttag gagaacttat
     2581 caaagatgca ctgcttcaag aagtagaaca aattattgtc actttaggag gaagcgggtg
     2641 tagcgatggt ggtctaggat tactccaatc cttgggtgct tctttagaag ggtacacaga
     2701 aggaaatcct ttattatcta ctaaaaaact tcattttgat cgaagaaacg ttcttgtttc
     2761 taaagtaaaa ttgattatcg ctgcagatgt taccaatcca tatacaggtc aacaaggagc
     2821 tgcttggcaa tttggaagac aaaaaggagg aatagatacg acgctagctt tcttagataa
     2881 acaagcagga aagattgctc agcaaataaa agatttctgg gaggtggatt tagatgatat
     2941 tccaggaagt ggagcagcag gcggtattgg tggagccctt tttttgttgg gagcgaagat
     3001 ggtttctggt ttcgatttag tcagccaaat cactggattg gaaaaaaaga tcgaacaagc
     3061 agatgtcatc attacaggag aagggcggat cgatcagcaa acgatccacg gaaaagttcc
     3121 acacggtgta gcagtgcttg caaaaaaata tcgaaaacca ataatcgcta tttgcggaag
     3181 tagagaccaa gagctagaag gactctctga ttttctccca attgtcttaa gtattcattt
     3241 gggtccaata ggtttagaag aagcaatgga acacaggcaa acattagaaa aaacaaaaat
     3301 cttaggacag acacttagca gatttttcac catcctcccg aagtagttga caaaatcatt
     3361 ttctttatgc tatcgtaatt tcaaatatag gacaaagtga gggatccgtg cattgaaaaa
     3421 ttaagtcgat tgatgaaagc tgcattcaga ctgtaccttg tggagacaag gaagcagtgt
     3481 gcagattttc gggattggct tggttttttc gatgtatggg tctttttgtg tgtatccaac
     3541 ccttgcatcc tctttagcca gttctgaaaa taggactggt ctttttttac gaggtgatgg
     3601 aaaatgtcta aaatcgaaat aaaaaatctg acattcggct acgacagcca aggcacatta
     3661 ttatttgaac aagcaaatct aaattttgac acacaatgga aactaggact tatcggacga
     3721 aacggtcgag gaaagacaac tttactgaat attctacaaa acaaactacc ttatcaaggg
     3781 caagtaatcc atcagcaaga atttgcctat ttcccgcaac agacaaaaga taaagaacgt
     3841 ttaacctatt acgtgttaaa tgatattacg gattttgaga tatgggaaat cgaaagagag
     3901 ctccaattga tgcaaacaga tcctgaaatc ttatggagag aattcagcac actatcgggg
     3961 ggagagaaga caaaagtcct actggcactt ttatttgtgg atgacactca tttcccgtta
     4021 atcgatgaac caacgaatca tttggatatc tctggtagaa aacaagtagc ggcttatttg
     4081 aaaaagaaaa aacaaggctt catcgtggtc agccatgacc ggggatttat cgatgaagta
     4141 gtggaccatg ttttagcaat cgaaaaaagt caactggaac tttatcaagg gaatttctct
     4201 atctatgaag aacagaaaaa acttcgtgat gaatttgaaa tggctcaaaa tgaaaaattg
     4261 aaaaaagaag tcagtaggct aaagaaaaca gcagctgaaa aagccgaatg gtctcgttcc
     4321 cgagaaggag ataaaacaaa gaaacaagtc ggattcatcg atactgaatc tagacgagtg
     4381 aataaaggag cagtgggtgc tgatgctgca cggacgatga aacgatccaa agcaatcgtg
     4441 aatcggatgg agacccagat cagcgagaaa gaaaaactat taaaagatat cgaatatatc
     4501 gattctttga cgatgaatag ccaagcgtct caccataagc gacttttaag cgtagaagat
     4561 cttcaattag ggtatgaaaa tctgttattc gagccaattc attttacaat cgagcctcat
     4621 cagcgggtgg cgatttcagg tcctaacggt gcaggaaagt catccattat ccattatctt
     4681 ctgggggcat tcaacggcaa ggttatagga gaaaaatccc agccaaaaca tctgagcatt
     4741 agttatgtac gccaaaatta tgaagacaat cgaggaacgt tggcggaatt tgcagagaaa
     4801 aaccaagtag actaccaagc atttttgaac aacctccgaa agcttgggat ggaaagagat
     4861 gtttttcata acaagatcga gcagatgagt atgggccaac ggaaaaaagt ggaattggct
     4921 aaatctttat cacagccagc tgaactatat acatgggatg aaccattgaa ttatttggat
     4981 gtcttcaatc aagaacaatt agaacaactg atcttgaacg tgaaacctgc catgttacta
     5041 gtggaacatg atcaaacctt cctggataaa gtatctactg agattatttc tcttgagaga
     5101 atctaaccct tgtaatctca agaacaacaa ggtatggtta aggggaactt tgccaacgaa
     5161 ggagagaatc acatgcttta tgtaacgaga cacggagaaa caacttggaa cgcacaagga
     5221 ttagtttgcg gacgggcaga tgtgccgctg actgaaaaag gacagatgca ggcacagaaa
     5281 ctagcagaaa aggtagtaga tttgcctgtt ccaatcacga aaatcatcca ttcccctctt
     5341 caacgagcaa gagatacagc acaagcagtt gctgatcgtc tcagcttgcc gctgaccgtt
     5401 gatgaacgat tggtagaaat ggattttggg gattatgatg gactgccaag caaagatgag
     5461 aactttcaaa aagcacgttt agcgttcgct gtacgattcc caaatgggga gtcagtctta
     5521 gatgtgtatg cacggatcgt tcctttattg aaagaatgca tcgaagacga agaaaatgtc
     5581 tatctgttag tttgccataa tgccttgata agagtgatca atgcttattt tcatccgatg
     5641 cctaatgagg ggttctttac attcatggta gataatacag aattggtttc ttatgagtaa
     5701 ttatgttttt gtgttattta aataaaatat ttcaaggcac tgcttttgct tagcagtgcc
     5761 ttttttaggt tatgacaatt cttttgataa tataaactca acctttagaa atgaaattta
     5821 attattttaa taaaagtatt gaaattttgt ttctttagat atatactcta gtcatagaaa
     5881 tgaagacgga tacagaaaaa gagggataat atgtttggct tttcaatatt tatgaacgat
     5941 tctttgtcag aagagaagaa acagtatatc cgccaaatgg catccaatgg ctttgtcggt
     6001 attttcactt ccatgcatat accagaagat gatgtttcgg catatcaaaa gcgattgatc
     6061 gaactaggaa gcgaagcgaa aaagaataaa ctagaattga tggtcgatat ttcaggagag
     6121 gcattggaac gagcgggttt ttcattagaa gatctaacgc ctttgaaagc aattggtgta
     6181 acaggtttaa gaatggatta tcatatttcc aatcagcaga ttgccgactg gtcacatcaa
     6241 ttgaaaatca gtttgaatgc tagcacgatc acgcctaaag atatcgatga attgaaagaa
     6301 gcagaagctg atttctcaca gattgaagct tggcataatt attatccacg tcccgaaaca
     6361 gggctggaca aagaatggta tcagaaaaaa aatcaatggc tcaaaattca aggtctcctt
     6421 gtccaaggtt ttgtaccagg agatacagag ttgagaggcc cactatatca aggattgcca
     6481 actttggaag aacatcgagg gatccatcct cttgcaggcg ctctagatct attagcaagt
     6541 aacacggata ttgtttacat cggagatgca ggtctgtcag aaaacgttca ggagcaattt
     6601 gcttctttcc aaaaagaaca gacagtcttg ctccatacgg aacctgtaga tgaagaattt
     6661 tacgaatata tattagggaa gcatacgaat cggcaagacg atgcaagaga tgtcatacga
     6721 agcgcagatg cacgttttcg agagattccg cctatcccag caagaaatac agctacgcgt
     6781 atgaaagggt ctattacttt agataacgaa aagtatttgc gctatatggg tgagatccaa
     6841 ttaaccaaat atgatttgcc agcggatgaa aaagtgaatg tagtagctaa agttatcaaa
     6901 gaagagctac cattgatcaa ccaaatcaaa gctggtatga actatcaatt tataagaaaa
     6961 gaaggaagat aaaattgagc aaaatcaatt tagagaacct aacaacggaa cgcagaaacc
     7021 aagaaacttt tggattagat gagatgagta tttctcaagc cgttcaacta atgaacaaag
     7081 aagatcagaa agtggccgct gcggttgaaa aagaactgac aagtatcgaa ccagtgattg
     7141 ctaacacgat tgagtccttt aaaaaaggtg gacgtctgat ttatatggga gccggaacaa
     7201 gtggaagact aggtgtgtta gatgctgctg aatgcgtacc gacattcggt gtagaaccgg
     7261 aaatggtcat aggtctaatt gctggtggag aagaagcaat gactgtagct gtcgaagggg
     7321 ccgaagattc ttatgagcta gggcaagaag atctaatcaa gctgaatttg acaaaaaatg
     7381 atatagttat tggtatcgca gctagcggtc gtacaccata tgtgatcggt ggattagatt
     7441 acgcaaaatc gatcggtgct gtcacaggaa cgatttcttg caataaacaa gccgaaatca
     7501 gtaaacacgc ggatttccca atcgaagtcg atgcaggtcc agaatttttg acaggctcta
     7561 ctcgattgaa gtcaggaaca gcacaaaaac ttatcttgaa tatgatttct actatcagta
     7621 tgattggtat aggtaaagta ttcaataact tgatggtcga tgtcaaacca acaaatgaaa
     7681 aactaatcga acgctcaaaa cgaatcatca tgcaagcgac agaagctgat tacgaaacag
     7741 ctgaaaagta ctttgaagaa gcagatcaaa atgttaagct ggctatcgtc atgattttga
     7801 caaatagtga taaagaggaa gctcaagcaa aattagctga agcaaacgga tttgtaaaag
     7861 ggaccttatg agggggaaaa gaccatggca aaaaacgaac tgactttgga agaactggca
     7921 aaacaaattt ataaaggggc aggcggcatg ggcaatgtag aaagcattgt ccactgcatg
     7981 actcgtgtgc gtatgaagat catcaatgat gatcttgtaa atgtctctga attaaaagcg
     8041 attccaggag tgcttggtgt agtagaagat gaacagcttc aagtcatcat cggacctggg
     8101 aaagtgaaca aagtagcaca gcaaatggtt gatttggcag gcgtgaaatt aggagaaaaa
     8161 ctttcttctg taagtgcagc tacagcaaat tcatcagcat ctggaaaaga ccgtgtaaac
     8221 gaacgggcac aagaaatgaa agcggcacaa aaatcgaaac aaaaaccatc taaaatcaaa
     8281 tcggtattaa aagacatttc taatatcttt gtgccaatga ttcctgcatt tgtcggtact
     8341 gggatcgtgg cagggattgc cgcagtttta gcaaacttag tcacagccgg aacacttgat
     8401 gcagcaacat ggcaacaata tatcgatgtg atgaacatct tgaaaaacgg gatgtttagc
     8461 tacctagtta tctatacagg tatcaatgct gcacaagttt ttggtgcaac accaactctt
     8521 ggtggagtta tcggggccgt tgtgatgctg acagggatga atccggaagc accactgaaa
     8581 aacttattta ctggtgaagc tcttgcagct ggacaaggtg ggatcctagg tgtcatcttc
     8641 gctgtttggc tattgtcaat cgttgagaaa aaactgcata aagtagtacc tgatgcggta
     8701 gatatcatcg ttacaccaac tctttcattg attgccatcg gtctgattga aattttcttc
     8761 atcatgcctt tagcaggttt catttctgat ggcatggtcg gtgggatcaa ctgggtactt
     8821 ggcgttggtg gagcatttgc cggcttcgtt ctaggcacat tattcttgcc gatggttatg
     8881 ttcggactgc atcaaatttt gactccaatc catgtgcaaa tgattgatga aactggtcgt
     8941 acgctattgc ttccaattct tgcgatggct ggtgcaggac aagtgggtgc agcattggca
     9001 ttatggattc gctgcaaaaa agataaagaa ttaacagaaa tgatcaaagg agctttacca
     9061 gtcgggatcc taggaatcgg cgaaccattg atttacggag taacattacc tctaggacgc
     9121 ccatttatta ctgcttgtat cggtggaggt atcggcgggg cagtgatcgg aatgatcgga
     9181 aatgtcgggg ctatcgctat tggaccttca ggagccgctt tgatcccatt gatctctgac
     9241 ggtaaatggt taggctacat ccttggatta ttggcagctt atgctggtgg cttcgttgct
     9301 acattcttct tcggtattcc taaagaccgt ttagaaaaag aagaattagc gactgaaaca
     9361 gccacagtaa atgaagtgcc tgaaaaagta gtacctcaaa cttcagctga aagtgtgact
     9421 atcctctcat cagttgctga cggaacagtt gtttctttag ataaagcaag tgatccagtt
     9481 tttgcacaaa agatgatggg tgaaggctac tttgtagaac cagaaaacgg acatatctat
     9541 tctccagtta ctggggtagt cagctctgtc ttcccaacga aacacgctat tgggatcaca
     9601 acgccaaatg gtttagaaat cttgcttcat atgggaatca acacagttga cttaggcggt
     9661 acgccatttg atgtgaaagt aacagaaggg cagcaagtga cacctgatac gcttgtagca
     9721 gaagctgact tagaagcgat caaagcagca ggcaaagaaa catccatgat cgtcttggta
     9781 accaatatgg atcgtgtaaa aaactttgtc ttggaaaaaa caggtaaagc aaaagcaaaa
     9841 gcacctgtga tggatgtcaa agcttaatat gatagaaaca atagaaacga aagaagaaac
     9901 tgaaatctat ttttcagctt cttctttttt attatttatt gttttctgct aaaatctgat
     9961 aaattcgtgt cacatcagat ggtgttcccc gtaattcata atctgccaag aaaggaaagc
    10021 caaatgtttg tgcatattgt ttggctgtta gacaatactg attattgaag tttttatttc
    10081 cgctaccgac tacaccaaga caaagtttat gattgtcttc atgttccaga tattcacgca
    10141 tcgtttcggt caagatttct gtatcaccgt tatctaaacc atttccgccg tctaaataag
    10201 taggaacgaa agtaaaaaat ggttctgttt ctttcgtaaa atcactattt tcatggattt
    10261 cttttaaagt aacttcagga ttgacaggat cttctgcatg cattttttca gcatattctg
    10321 ctagatgttt ggcaaaggca cgcgtatttc ctgaaatcga tatatataaa atgttcatgg
    10381 atcatcctcc aattctttcc ttttagtata gcatcttttt taagtttttt ggtggtgtaa
    10441 tttggaaagg gactaggcgc tttgcttgta taatgagaaa aaagccggta gaattatatt
    10501 tctattaaag tttttattaa ttaagtaaaa ataaaaaagg tttgttatac tttttgataa
    10561 aagcggtttt attttggagg atgcttacat ggatacagag caacagaaaa aaggaattgg
    10621 tttaattcca cttgctgcct tagtaattgg ttcagcgatt ggaggtggcg tatttggtat
    10681 tatgacggac atctccggat cggctggcgg cccagctatt ttcagttggg tactcgtagg
    10741 aacagcaatg ttgatgctct ccttgtcgat caataatatc aataaaaaac gtccagattt
    10801 aaaaggcggg atctttagtt acgcagaaga aggttttggc cgtttcgctg gttttatcag
    10861 cggttggggt tactggctca catgctggtt aggcaacgta gcttttgcaa cattgttgat
    10921 gagcgcatta ggatatttct tcccagtatt tgaaggtggg caaaatctgg catcgatcgt
    10981 gatgagtaca gtctttttat ggggatatgc atggctagtg aatcggggag tcgaaaatgc
    11041 tagtttcgtc aatgctatcg tcacattctg taaattagtg ccattatttt tatttattgt
    11101 agtagctatc agcacgtttt cgatccaaca attcattgat aacttttcta gcgacattat
    11161 attgaatgcg aatggacagc cgatcccgtt ttccagtcaa gtattgagtt gcatcatggt
    11221 gatgctttgg gtatttgttg gtattgaagg agcctctgtc gtaggttcac gagcaaaaaa
    11281 acaatcagat gttgggaaag caacgattct agggatattc ggcttgatta ttatttacgt
    11341 cttggtttcc atgcttccat atgggacaat gacgatgaat cagctgcaaa cgatcaaggc
    11401 gcagcctgct atgggctatg tgtttgaaat gatggttggt aaatggggag cagtcgtgat
    11461 caacggcggt ttgatcattt ctttagttgg tgtttggctc tcatggacga tcttaccgat
    11521 tgaaacaatg ggaaacatgg ctgaagacgg acttttgcct aaagcatggg gaaaactcaa
    11581 caaaaatggg gcaccaactt atgctatcgt attgacgacg ttatgtacga atgtcttttt
    11641 actttcatta ttattcactg acagtgctta taactttgct tacacacttg gtacagcagc
    11701 tattttcttc acgtggcttt tcttaggatt gtaccaaatg aaactttctt atcagcggaa
    11761 agaatgggtc caatttttag tagggctttt agcaagtgcc tttcaagtat gggctatgct
    11821 agtcgtagcc tttaatgaag taatgcttgt tcttgttctt ttcttgccag gcatttattt
    11881 ttacctcaaa gcacgtaagg agcaaaacga gcctgtacaa catatccact tagataagat
    11941 tttacttata ggtgtgacga tggtcggagt gattgcaatg gttttatttg ccactgggat
    12001 gcttcacgta tagaagtata aaagaagtat aaagagaggt tgtgagcctg tcgtttattc
    12061 aaaaacatac taggaaagga gctgtgacac aaatgttgtc acagctcctt tttcagaata
    12121 aacggtgttt aagagacagc caatcggcaa taaggctgaa cgtctctttc acagcctctt
    12181 ttttgatgag tcatcataca ttttttcgct taaatacgag atatcctaat gctaagaaca
    12241 aaacgatgta agctaaatta ccgaatagca tttgattagt agaaagatga gtcaattgct
    12301 tcatgatctc ttcgtttcca atttggtttt gcagattcaa catactgatc ggattccatt
    12361 tgatccactc ccatttttgg atgagtgcaa taaggatacc agatatgatt gacgaagcaa
    12421 aataaaagac gattcctgct gagatggatg caccggaact attgataaag caagcaatca
    12481 tcaacactaa acttaaaatc agccataaac cgatataact acccaaagta taagtgaaca
    12541 agctttggat cagtgtttga ccagtagaaa ccttctccgt gaaggaaatc gaagggaaaa
    12601 gaatcagttt catcaacaca gtgacaataa tagctaaaag gtataaataa acagaataaa
    12661 taactaatgt gatccatttg cttactaata tttctcctcg agaatacttt cgataaagga
    12721 gatttttcaa tgtaccatat tgtgcttcca tcgaaatgat cgtgcttgca gctgcaatca
    12781 tgataaagac aatccaagat gtggcactaa aaagctgagc agtcatttca actggatcaa
    12841 tgatcgtcgt gtattttttg gctagaagtg ctgttcctat gagcaaaaca actaatagaa
    12901 cagataaaac agcagttgac tttttcttga tcaatttaaa taattcttgt tttacaagca
    12961 atgtcatttt tcttcttccc cctttactga atccagaatt tccaatagag aagattccaa
    13021 atcattcgtc agatgctgga cgtctgtcac atggatattt tgtgcagaca atagctggat
    13081 cacggttcct aataattctt cgttttcttt taaaagcttg attttatcag gatcttccaa
    13141 agtatatcca gcttctgtca agatcgttcg agcagctaga tcatttgttg tagagataac
    13201 gatgaaatct tgcgctgttt ggttcaattc agccattgta cattgtttga tgatctttcc
    13261 ttgattgatc aggatcagct catcgaccat tttttcaagc tcgctgagta agtgactgga
    13321 gatcaagaaa gaggtgccct gagtagccaa gtcggtaatg atttttcgca catctcttgt
    13381 tgcttgtgga tccaaaccgt tcatcggctc atctaaaata acgatagacg gatggttcag
    13441 taaggcaaga gcaattccca atttttgttt cattcccaac gagtaggatt tcgctttttt
    13501 gtgaatgtac ttttccatct tgagctgatt cacgatagtg tcgatagcag atacatcgtt
    13561 ggtttcagag aataacttca gatgatcata gcctgttaag aatggataga tacccgggta
    13621 ttcgatcaat gctcctactt gttccaaccc tttatgacta gagggagaaa tctcattttc
    13681 accaatacag attgttccat ctgagtaatg gatcagtcct aaaatagctt tcatgatcgt
    13741 tgtttttcca gcgccattag gacctactaa tccaacgatt ttgccagggg ataagctaaa
    13801 actgacatcg ctgagggctt gtttgtgacc aaagttcttg gataattctt ggaccgaaag
    13861 acttttcaaa aatattcact ccttttctat agatagtaca agcatacacc tgatgagtgg
    13921 agaagtcgct aggtcattgg acttatttct ta
//
LOCUS       DAZUMM010000246        13951 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.guided.067, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000246 DAZUMM010000000
VERSION     DAZUMM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.guided.067"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            113..1009
                     /locus_tag="ACG8E6_002830"
     CDS             113..1009
                     /locus_tag="ACG8E6_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS5063149.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFNMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLAYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPIPSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1159..2100)
                     /locus_tag="ACG8E6_002831"
     CDS             complement(1159..2100)
                     /locus_tag="ACG8E6_002831"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS5063150.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFRSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKTAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2225..3346
                     /locus_tag="ACG8E6_002832"
     CDS             2225..3346
                     /locus_tag="ACG8E6_002832"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS5063151.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPHGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3604..5105
                     /gene="eat(A)"
                     /locus_tag="ACG8E6_002833"
                     /pseudo
     CDS             3604..5105
                     /gene="eat(A)"
                     /locus_tag="ACG8E6_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
     gene            5172..5699
                     /locus_tag="ACG8E6_002834"
     CDS             5172..5699
                     /locus_tag="ACG8E6_002834"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5063152.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5910..6971
                     /locus_tag="ACG8E6_002835"
     CDS             5910..6971
                     /locus_tag="ACG8E6_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS5063153.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6974..7870
                     /gene="murQ"
                     /locus_tag="ACG8E6_002836"
     CDS             6974..7870
                     /gene="murQ"
                     /locus_tag="ACG8E6_002836"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS5063154.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7884..9866
                     /locus_tag="ACG8E6_002837"
     CDS             7884..9866
                     /locus_tag="ACG8E6_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS5063155.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSAATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9931..10377)
                     /gene="nrdI"
                     /locus_tag="ACG8E6_002838"
     CDS             complement(9931..10377)
                     /gene="nrdI"
                     /locus_tag="ACG8E6_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS5063156.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10588..12012
                     /locus_tag="ACG8E6_002839"
     CDS             10588..12012
                     /locus_tag="ACG8E6_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFS5063157.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12193..12966)
                     /locus_tag="ACG8E6_002840"
     CDS             complement(12193..12966)
                     /locus_tag="ACG8E6_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5063158.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12963..13868)
                     /locus_tag="ACG8E6_002841"
     CDS             complement(12963..13868)
                     /locus_tag="ACG8E6_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5063159.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
BASE COUNT         4420 a         2478 c         3000 g         4053 t
ORIGIN      
        1 ttttcataca aatgttaaat tttcatacaa atataggaag cgttttactg gctttttact
       61 atttaaacag gctatcatag gaaaaagtga cagagaaagt gaggcggaaa agatgaacaa
      121 aaatgacgta ctgctagttg caaaaaataa atcactttat tttttgcctt ccatccgctc
      181 tttagatgtt tggtccggaa aagatttact acagtcggaa ttcattaaat cagctagaca
      241 acagttggca aattatcagc tgatcgtttt tttagattat ggatttaata tggagatggc
      301 tcgacgcgtg cgcccattta ccaatgcaaa aattgtgtta tttttttgga atcacttcaa
      361 agcagaacac gaggcgatgc tgaagcaggc acaagatgaa ccggcaatcg atgagattta
      421 tcattttgat tttttggaag caaaagaact tgggctgaaa cataatagtt cattttattc
      481 gaaacatatg gatttttctt ctagcgaacc aaagacggat ctattttttg gtgcaacgaa
      541 taatgggaga aaagaacgag cagaaagcta taagcaggaa tttataaaaa gagatgttac
      601 gaccaactat ttcattcttc cacatcgagg gaatgaacaa cctggatatc ttgcttatga
      661 agagtacctg aaaaaaacag gggaatcgcg cgggattctg gaactgttgc gggagggtca
      721 gcaaggagtg actttgcgga catttgaatc tatgtatttt caaaagaaac tagtgacgga
      781 taatcaagcg attgctcatt atcattttta tcgtcctgaa aatatttttt tgcttcgaga
      841 acgtgattta gatgaattac cggaatttct gcatacacct tttaagccaa tcccatcaga
      901 gctattggaa ttttatgatg cagaaaattg ggcaaaacga tttatcacta acgattccac
      961 tatttatgag caatacgaat atgacctctc ctctgttgaa aataaataaa aaacctacat
     1021 tttgaagaga ttatgtaccg aaacaaacaa gcacaattct tatggcatca tgagagttgt
     1081 gttttctttt tgatctttca ttgggcttaa aaaaagagaa ccagctgctg attcagctga
     1141 ttctcatcag agagcctttt attctgcgcc aaggattcct tcttttgcat ggactaattg
     1201 ggtcaaaaag gcacaatatg gtgtagcaat atcatatttt tgtccttttc gccaaacggc
     1261 accattgatg tagtcgattt cagtcaaacg atggtttttg atcaggtctt ggtacataga
     1321 tgggtaatgc aacccgatgt tgtcaggatc ataagtagaa gcaatatgtt ggtacacttc
     1381 ttcttgatct aatatgattc cttcttttgc tgcgatagaa gcaaattcag atacgattgt
     1441 tcgaaccatg ctctctgccg ttttttgcgc gcctagttca gcaatattac aatccaaaat
     1501 cgtgcataaa ccattcaatg ttccatttac acaagctttt cgccaaatgg aataacgaac
     1561 attatggcta tagatcggat ttaattttgc ttcttgaaag acttccacaa ctttttttgt
     1621 aaatgcttcg ccttctggtt ccaagttctc tagttcgatt tctccatcac ctaacaatgt
     1681 gacttttccg ggaccagcta gaccagctgt ccacatcgtg atcccaaata agatattttt
     1741 ctttggcaca tatttttcca gtacatcttc atgacctaat ccatttagta aacacaaaac
     1801 ataagtattg tcagtaataa tggattggat cgaacgaaac atatcatcta attgattcgc
     1861 ttttgttaat gctacgatca agtccacgtg ttcgttgctt tcgatgattt cttctggtga
     1921 atagattggc agttttgcga ccacttcttt accgttaaag tcagcaatca acccatttgt
     1981 acgaatggct tcaatgtgtg caggccaacg atcgatcaag aggacctcat ttccgctttg
     2041 atgtagcatc aatccgattc ggctgcccat tgctccggct ccagctattg cgattttcat
     2101 gttttttctc tcctttatca atgagttccc ttttagtata tagaaaaatg gaagaaaata
     2161 gcattgtttt gtttgaaaaa aatgaaaaaa agtgtcttac ttaagaagaa agtgagggat
     2221 acagatggat attttggcgg caattgattc atttaaagga agtgcttctt catttgaact
     2281 aaatcaggca gttctaaatg gctgggaaga tcgtaaagga agaaaaatca atgtgccgat
     2341 tgcagatggc ggagaaaata ctgcagaagc aatctattac gcgctcgggg gaacatggcg
     2401 caccattgat gggatggatc ttttattccg tcagcgaaaa atcagctatt tattgacaac
     2461 ttggcaagga aaaaataccg cagtcatcga atctgcacaa gcattggggt tagatctact
     2521 tgataaacca acggatcaaa ctatccgact tgcttccagt tatgccttag gagaacttat
     2581 caaagatgca ctgcttcaag aagtagaaca aattattgtc actttaggag gaagcgggtg
     2641 tagcgatggt ggtctaggat tactccaatc cttgggtgct tctttagaag ggtacacaga
     2701 aggaaatcct ttattatcta ctaaaaaact tcattttgat cgaagaaacg ttcttgtttc
     2761 taaagtaaaa ttgattatcg ctgcagatgt taccaatcca tatacaggtc aacaaggagc
     2821 tgcttggcaa tttggaagac aaaaaggagg aatagatacg acgctagctt tcttagataa
     2881 acaagcagga aagattgctc agcaaataaa agatttctgg gaggtggatt tagatgatat
     2941 tccaggaagt ggagcagcag gcggtattgg tggagccctt tttttgttgg gagcgaagat
     3001 ggtttctggt ttcgatttag tcagccaaat cactggattg gaaaaaaaga tcgaacaagc
     3061 agatgtcatc attacaggag aagggcggat cgatcagcaa acgatccacg gaaaagttcc
     3121 acacggtgta gcagtgcttg caaaaaaata tcgaaaacca ataatcgcta tttgcggaag
     3181 tagagaccaa gagctagaag gactctctga ttttctccca attgtcttaa gtattcattt
     3241 gggtccaata ggtttagaag aagcaatgga acacaggcaa acattagaaa aaacaaaaat
     3301 cttaggacag acacttagca gatttttcac catcctcccg aagtagttga caaaatcatt
     3361 ttctttatgc tatcgtaatt tcaaatatag gacaaagtga gggatccgtg cattgaaaaa
     3421 ttaagtcgat tgatgaaagc tgcattcaga ctgtaccttg tggagacaag gaagcagtgt
     3481 gcagattttc gggattggct tggttttttc gatgtatggg tctttttgtg tgtatccaac
     3541 ccttgcatcc tctttagcca gttctgaaaa taggactggt ctttttttac gaggtgatgg
     3601 aaaatgtcta aaatcgaaat aaaaaatctg acattcggct acgacagcca aggcacatta
     3661 ttatttgaac aagcaaatct aaattttgac acacaatgga aactaggact tatcggacga
     3721 aacggtcgag gaaagacaac tttactgaat attctacaaa acaaactacc ttatcaaggg
     3781 caagtaatcc atcagcaaga atttgcctat ttcccgcaac agacaaaaga taaagaacgt
     3841 ttaacctatt acgtgttaaa tgatattacg gattttgaga tatgggaaat cgaaagagag
     3901 ctccaattga tgcaaacaga tcctgaaatc ttatggagag aattcagcac actatcgggg
     3961 ggagagaaga caaaagtcct actggcactt ttatttgtgg atgacactca tttcccgtta
     4021 atcgatgaac caacgaatca tttggatatc tctggtagaa aacaagtagc ggcttatttg
     4081 aaaaagaaaa aacaaggctt catcgtggtc agccatgacc ggggatttat cgatgaagta
     4141 gtggaccatg ttttagcaat cgaaaaagtc aactggaact ttatcaaggg aatttctcta
     4201 tctatgaaga acagaaaaaa cttcgtgatg aatttgaaat ggctcaaaat gaaaaattga
     4261 aaaaagaagt cagtaggcta aagaaaacag cagctgaaaa agccgaatgg tctcgttccc
     4321 gagaaggaga taaaacaaag aaacaagtcg gattcatcga tactgaatct agacgagtga
     4381 ataaaggagc agtgggtgct gatgctgcac ggacgatgaa acgatccaaa gcaatcgtga
     4441 atcggatgga gacccagatc agcgagaaag aaaaactatt aaaagatatc gaatatatcg
     4501 attctttgac gatgaatagc caagcgtctc accataagcg acttttaagc gtagaagatc
     4561 ttcaattagg gtatgaaaat ctgttattcg agccaattca ttttacaatc gagcctcatc
     4621 agcgggtggc gatttcaggt cctaacggtg caggaaagtc atccattatc cattatcttc
     4681 tgggggcatt caacggcaag gttataggag aaaaatccca gccaaaacat ctgagcatta
     4741 gttatgtacg ccaaaattat gaagacaatc gaggaacgtt ggcggaattt gcagagaaaa
     4801 accaagtaga ctaccaagca tttttgaaca acctccgaaa gcttgggatg gaaagagatg
     4861 tttttcataa caagatcgag cagatgagta tgggccaacg gaaaaaagtg gaattggcta
     4921 aatctttatc acagccagct gaactatata catgggatga accattgaat tatttggatg
     4981 tcttcaatca agaacaatta gaacaactga tcttgaacgt gaaacctgcc atgttactag
     5041 tggaacatga tcaaaccttc ctggataaag tatctactga gattatttct cttgagagaa
     5101 tctaaccctt gtaatctcaa gaacaacaag gtatggttaa ggggaacttt gccaacgaag
     5161 gagagaatca catgctttat gtaacgagac acggagaaac aacttggaac gcacaaggat
     5221 tagtttgcgg acgggcagat gtgccgctga ctgaaaaagg acagatgcag gcacagaaac
     5281 tagcagaaaa ggtagtagat ttgcctgttc caatcacgaa aatcatccat tcccctcttc
     5341 aacgagcaag agatacagca caagcagttg ctgatcgtct cagcttgccg ctgaccgttg
     5401 atgaacgatt ggtagaaatg gattttgggg attatgatgg actgccaagc aaagatgaga
     5461 actttcaaaa agcacgttta gcgttcgctg tacgattccc aaatggggag tcagtcttag
     5521 atgtgtatgc acggatcgtt cctttattga aagaatgcat cgaagacgaa gaaaatgtct
     5581 atctgttagt ttgccataat gccttgataa gagtgatcaa tgcttatttt catccgatgc
     5641 ctaatgaggg gttctttaca ttcatggtag ataatacaga attggtttct tatgagtaat
     5701 tatgtttttg tgttatttaa ataaaatatt tcaaggcact gcttttgctt agcagtgcct
     5761 tttttaggtt atgacaattc ttttgataat ataaactcaa cctttagaaa tgaaatttaa
     5821 ttattttaat aaaagtattg aaattttgtt tctttagata tatactctag tcatagaaat
     5881 gaagacggat acagaaaaag agggataata tgtttggctt ttcaatattt atgaacgatt
     5941 ctttgtcaga agagaagaaa cagtatatcc gccaaatggc atccaatggc tttgtcggta
     6001 ttttcacttc catgcatata ccagaagatg atgtttcggc atatcaaaag cgattgatcg
     6061 aactaggaag cgaagcgaaa aagaataaac tagaattgat ggtcgatatt tcaggagagg
     6121 cattggaacg agcgggtttt tcattagaag atctaacgcc tttgaaagca attggtgtaa
     6181 caggtttaag aatggattat catatttcca atcagcagat tgccgactgg tcacatcaat
     6241 tgaaaatcag tttgaatgct agcacgatca cgcctaaaga tatcgatgaa ttgaaagaag
     6301 cagaagctga tttctcacag attgaagctt ggcataatta ttatccacgt cccgaaacag
     6361 ggctggacaa agaatggtat cagaaaaaaa atcaatggct caaaattcaa ggtctccttg
     6421 tccaaggttt tgtaccagga gatacagagt tgagaggccc actatatcaa ggattgccaa
     6481 ctttggaaga acatcgaggg atccatcctc ttgcaggcgc tctagatcta ttagcaagta
     6541 acacggatat tgtttacatc ggagatgcag gtctgtcaga aaacgttcag gagcaatttg
     6601 cttctttcca aaaagaacag acagtcttgc tccatacgga acctgtagat gaagaatttt
     6661 acgaatatat attagggaag catacgaatc ggcaagacga tgcaagagat gtcatacgaa
     6721 gcgcagatgc acgttttcga gagattccgc ctatcccagc aagaaataca gctacgcgta
     6781 tgaaagggtc tattacttta gataacgaaa agtatttgcg ctatatgggt gagatccaat
     6841 taaccaaata tgatttgcca gcggatgaaa aagtgaatgt agtagctaaa gttatcaaag
     6901 aagagctacc attgatcaac caaatcaaag ctggtatgaa ctatcaattt ataagaaaag
     6961 aaggaagata aaattgagca aaatcaattt agagaaccta acaacggaac gcagaaacca
     7021 agaaactttt ggattagatg agatgagtat ttctcaagcc gttcaactaa tgaacaaaga
     7081 agatcagaaa gtggccgctg cggttgaaaa agaactgaca agtatcgaac cagtgattgc
     7141 taacacgatt gagtccttta aaaaaggtgg acgtctgatt tatatgggag ccggaacaag
     7201 tggaagacta ggtgtgttag atgctgctga atgcgtaccg acattcggtg tagaaccgga
     7261 aatggtcata ggtctaattg ctggtggaga agaagcaatg actgtagctg tcgaaggggc
     7321 cgaagattct tatgagctag ggcaagaaga tctaatcaag ctgaatttga caaaaaatga
     7381 tatagttatt ggtatcgcag ctagcggtcg tacaccatat gtgatcggtg gattagatta
     7441 cgcaaaatcg atcggtgctg tcacaggaac gatttcttgc aataaacaag ccgaaatcag
     7501 taaacacgcg gatttcccaa tcgaagtcga tgcaggtcca gaatttttga caggctctac
     7561 tcgattgaag tcaggaacag cacaaaaact tatcttgaat atgatttcta ctatcagtat
     7621 gattggtata ggtaaagtat tcaataactt gatggtcgat gtcaaaccaa caaatgaaaa
     7681 actaatcgaa cgctcaaaac gaatcatcat gcaagcgaca gaagctgatt acgaaacagc
     7741 tgaaaagtac tttgaagaag cagatcaaaa tgttaagctg gctatcgtca tgattttgac
     7801 aaatagtgat aaagaggaag ctcaagcaaa attagctgaa gcaaacggat ttgtaaaagg
     7861 gaccttatga gggggaaaag accatggcaa aaaacgaact gactttggaa gaactggcaa
     7921 aacaaattta taaaggggca ggcggcatgg gcaatgtaga aagcattgtc cactgcatga
     7981 ctcgtgtgcg tatgaagatc atcaatgatg atcttgtaaa tgtctctgaa ttaaaagcga
     8041 ttccaggagt gcttggtgta gtagaagatg aacagcttca agtcatcatc ggacctggga
     8101 aagtgaacaa agtagcacag caaatggttg atttggcagg cgtgaaatta ggagaaaaac
     8161 tttcttctgt aagtgcagct acagcaaatt catcagcatc tggaaaagac cgtgtaaacg
     8221 aacgggcaca agaaatgaaa gcggcacaaa aatcgaaaca aaaaccatct aaaatcaaat
     8281 cggtattaaa agacatttct aatatctttg tgccaatgat tcctgcattt gtcggtactg
     8341 ggatcgtggc agggattgcc gcagttttag caaacttagt cacagccgga acacttgatg
     8401 cagcaacatg gcaacaatat atcgatgtga tgaacatctt gaaaaacggg atgtttagct
     8461 acctagttat ctatacaggt atcaatgctg cacaagtttt tggtgcaaca ccaactcttg
     8521 gtggagttat cggggccgtt gtgatgctga cagggatgaa tccggaagca ccactgaaaa
     8581 acttatttac tggtgaagct cttgcagctg gacaaggtgg gatcctaggt gtcatcttcg
     8641 ctgtttggct attgtcaatc gttgagaaaa aactgcataa agtagtacct gatgcggtag
     8701 atatcatcgt tacaccaact ctttcattga ttgccatcgg tctgattgaa attttcttca
     8761 tcatgccttt agcaggtttc atttctgatg gcatggtcgg tgggatcaac tgggtacttg
     8821 gcgttggtgg agcatttgcc ggcttcgttc taggcacatt attcttgccg atggttatgt
     8881 tcggactgca tcaaattttg actccaatcc atgtgcaaat gattgatgaa actggtcgta
     8941 cgctattgct tccaattctt gcgatggctg gtgcaggaca agtgggtgca gcattggcat
     9001 tatggattcg ctgcaaaaaa gataaagaat taacagaaat gatcaaagga gctttaccag
     9061 tcgggatcct aggaatcggc gaaccattga tttacggagt aacattacct ctaggacgcc
     9121 catttattac tgcttgtatc ggtggaggta tcggcggggc agtgatcgga atgatcggaa
     9181 atgtcggggc tatcgctatt ggaccttcag gagccgcttt gatcccattg atctctgacg
     9241 gtaaatggtt aggctacatc cttggattat tggcagctta tgctggtggc ttcgttgcta
     9301 cattcttctt cggtattcct aaagaccgtt tagaaaaaga agaattagcg actgaaacag
     9361 ccacagtaaa tgaagtgcct gaaaaagtag tacctcaaac ttcagctgaa agtgtgacta
     9421 tcctctcatc agttgctgac ggaacagttg tttctttaga taaagcaagt gatccagttt
     9481 ttgcacaaaa gatgatgggt gaaggctact ttgtagaacc agaaaacgga catatctatt
     9541 ctccagttac tggggtagtc agctctgtct tcccaacgaa acacgctatt gggatcacaa
     9601 cgccaaatgg tttagaaatc ttgcttcata tgggaatcaa cacagttgac ttaggcggta
     9661 cgccatttga tgtgaaagta acagaagggc agcaagtgac acctgatacg cttgtagcag
     9721 aagctgactt agaagcgatc aaagcagcag gcaaagaaac atccatgatc gtcttggtaa
     9781 ccaatatgga tcgtgtaaaa aactttgtct tggaaaaaac aggtaaagca aaagcaaaag
     9841 cacctgtgat ggatgtcaaa gcttaatatg atagaaacaa tagaaacgaa agaagaaact
     9901 gaaatctatt tttcagcttc ttctttttta ttatttattg ttttctgcta aaatctgata
     9961 aattcgtgtc acatcagatg gtgttccccg taattcataa tctgccaaga aaggaaagcc
    10021 aaatgtttgt gcatattgtt tggctgttag acaatactga ttattgaagt ttttatttcc
    10081 gctaccgact acaccaagac aaagtttatg attgtcttca tgttccagat attcacgcat
    10141 cgtttcggtc aagatttctg tatcaccgtt atctaaacca tttccgccgt ctaaataagt
    10201 aggaacgaaa gtaaaaaatg gttctgtttc tttcgtaaaa tcactatttt catggatttc
    10261 ttttaaagta acttcaggat tgacaggatc ttctgcatgc attttttcag catattctgc
    10321 tagatgtttg gcaaaggcac gcgtatttcc tgaaatcgat atatataaaa tgttcatgga
    10381 tcatcctcca attctttcct tttagtatag catctttttt aagttttttg gtggtgtaat
    10441 ttggaaaggg actaggcgct ttgcttgtat aatgagaaaa aagccggtag aattatattt
    10501 ctattaaagt ttttattaat taagtaaaaa taaaaaaggt ttgttatact ttttgataaa
    10561 agcggtttta ttttggagga tgcttacatg gatacagagc aacagaaaaa aggaattggt
    10621 ttaattccac ttgctgcctt agtaattggt tcagcgattg gaggtggcgt atttggtatt
    10681 atgacggaca tctccggatc ggctggcggc ccagctattt tcagttgggt actcgtagga
    10741 acagcaatgt tgatgctctc cttgtcgatc aataatatca ataaaaaacg tccagattta
    10801 aaaggcggga tctttagtta cgcagaagaa ggttttggcc gtttcgctgg ttttatcagc
    10861 ggttggggtt actggctcac atgctggtta ggcaacgtag cttttgcaac attgttgatg
    10921 agcgcattag gatatttctt cccagtattt gaaggtgggc aaaatctggc atcgatcgtg
    10981 atgagtacag tctttttatg gggatatgca tggctagtga atcggggagt cgaaaatgct
    11041 agtttcgtca atgctatcgt cacattctgt aaattagtgc cattattttt atttattgta
    11101 gtagctatca gcacgttttc gatccaacaa ttcattgata acttttctag cgacattata
    11161 ttgaatgcga atggacagcc gatcccgttt tccagtcaag tattgagttg catcatggtg
    11221 atgctttggg tatttgttgg tattgaagga gcctctgtcg taggttcacg agcaaaaaaa
    11281 caatcagatg ttgggaaagc aacgattcta gggatattcg gcttgattat tatttacgtc
    11341 ttggtttcca tgcttccata tgggacaatg acgatgaatc agctgcaaac gatcaaggcg
    11401 cagcctgcta tgggctatgt gtttgaaatg atggttggta aatggggagc agtcgtgatc
    11461 aacggcggtt tgatcatttc tttagttggt gtttggctct catggacgat cttaccgatt
    11521 gaaacaatgg gaaacatggc tgaagacgga cttttgccta aagcatgggg aaaactcaac
    11581 aaaaatgggg caccaactta tgctatcgta ttgacgacgt tatgtacgaa tgtcttttta
    11641 ctttcattat tattcactga cagtgcttat aactttgctt acacacttgg tacagcagct
    11701 attttcttca cgtggctttt cttaggattg taccaaatga aactttctta tcagcggaaa
    11761 gaatgggtcc aatttttagt agggctttta gcaagtgcct ttcaagtatg ggctatgcta
    11821 gtcgtagcct ttaatgaagt aatgcttgtt cttgttcttt tcttgccagg catttatttt
    11881 tacctcaaag cacgtaagga gcaaaacgag cctgtacaac atatccactt agataagatt
    11941 ttacttatag gtgtgacgat ggtcggagtg attgcaatgg ttttatttgc cactgggatg
    12001 cttcacgtat agaagtataa aagaagtata aagagaggtt gtgagcctgt cgtttattca
    12061 aaaacatact aggaaaggag ctgtgacaca aatgttgtca cagctccttt ttcagaataa
    12121 acggtgttta agagacagcc aatcggcaat aaggctgaac gtctctttca cagcctcttt
    12181 tttgatgagt catcatacat tttttcgctt aaatacgaga tatcctaatg ctaagaacaa
    12241 aacgatgtaa gctaaattac cgaatagcat ttgattagta gaaagatgag tcaattgctt
    12301 catgatctct tcgtttccaa tttggttttg cagattcaac atactgatcg gattccattt
    12361 gatccactcc catttttgga tgagtgcaat aaggatacca gatatgattg acgaagcaaa
    12421 ataaaagacg attcctgctg agatggatgc accggaacta ttgataaagc aagcaatcat
    12481 caacactaaa cttaaaatca gccataaacc gatataacta cccaaagtat aagtgaacaa
    12541 gctttggatc agtgtttgac cagtagaaac cttctccgtg aaggaaatcg aagggaaaag
    12601 aatcagtttc atcaacacag tgacaataat agctaaaagg tataaataaa cagaataaat
    12661 aactaatgtg atccatttgc ttactaatat ttctcctcga gaatactttc gataaaggag
    12721 atttttcaat gtaccatatt gtgcttccat cgaaatgatc gtgcttgcag ctgcaatcat
    12781 gataaagaca atccaagatg tggcactaaa aagctgagca gtcatttcaa ctggatcaat
    12841 gatcgtcgtg tattttttgg ctagaagtgc tgttcctatg agcaaaacaa ctaatagaac
    12901 agataaaaca gcagttgact ttttcttgat caatttaaat aattcttgtt ttacaagcaa
    12961 tgtcattttt cttcttcccc ctttactgaa tccagaattt ccaatagaga agattccaaa
    13021 tcattcgtca gatgctggac gtctgtcaca tggatatttt gtgcagacaa tagctggatc
    13081 acggttccta ataattcttc gttttctttt aaaagcttga ttttatcagg atcttccaaa
    13141 gtatatccag cttctgtcaa gatcgttcga gcagctagat catttgttgt agagataacg
    13201 atgaaatctt gcgctgtttg gttcaattca gccattgtac attgtttgat gatctttcct
    13261 tgattgatca ggatcagctc atcgaccatt ttttcaagct cgctgagtaa gtgactggag
    13321 atcaagaaag aggtgccctg agtagccaag tcggtaatga tttttcgcac atctcttgtt
    13381 gcttgtggat ccaaaccgtt catcggctca tctaaaataa cgatagacgg atggttcagt
    13441 aaggcaagag caattcccaa tttttgtttc attcccaacg agtaggattt cgcttttttg
    13501 tgaatgtact tttccatctt gagctgattc acgatagtgt cgatagcaga tacatcgttg
    13561 gtttcagaga ataacttcag atgatcatag cctgttaaga atggatagat acccgggtat
    13621 tcgatcaatg ctcctacttg ttccaaccct ttatgactag agggagaaat ctcattttca
    13681 ccaatacaga ttgttccatc tgagtaatgg atcagtccta aaatagcttt catgatcgtt
    13741 gtttttccag cgccattagg acctactaat ccaacgattt tgccagggga taagctaaaa
    13801 ctgacatcgc tgagggcttg tttgtgacca aagttcttgg ataattcttg gaccgaaaga
    13861 cttttcaaaa atattcactc cttttctata gatagtacaa gcatacacct gatgagtgga
    13921 gaagtcgcta ggtcattgga cttatttctt a
//
LOCUS       DAZUMM010000247        10166 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.guided.088, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000247 DAZUMM010000000
VERSION     DAZUMM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.guided.088"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2413
                     /locus_tag="ACG8E6_002842"
     CDS             <1..2413
                     /locus_tag="ACG8E6_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFS5063160.1"
                     /translation="LNHLELIGTPVYGAIIKMDILRTPLADDSHKEIARGFDRLKQFQ
                     AFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKKLALLVAFIFIYKRKAMDE
                     QILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSDIVDLVMSSHSDTNELSLR
                     DRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYRKFRKFIPDILTTLTFDSN
                     DYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYYIHEYPEQTNKCVLIAAVE
                     LLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLLEQLDLPSSANEMSLWLKN
                     DLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKEDKSFRRRIQQLLPKIDLS
                     DLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSESCNIGFSPVSKEGFDSLK
                     YDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDGHMASADGIRYITPQRSLY
                     SRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSLYLLEGFLNQPSELTPNQI
                     MTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDANAHYGELDTLSQNSINMEL
                     IYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGKAITEYGKVYKTKHQLRYI
                     SDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYDGMEEQLNALGLVTNAIIY
                     WNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVGKYTFRYEAELSNGKLRPL
                     NVEEF"
     gene            2650..2874
                     /locus_tag="ACG8E6_002843"
     CDS             2650..2874
                     /locus_tag="ACG8E6_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5063161.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            2889..3758
                     /locus_tag="ACG8E6_002844"
     CDS             2889..3758
                     /locus_tag="ACG8E6_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS5063162.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3739..4473
                     /locus_tag="ACG8E6_002845"
     CDS             3739..4473
                     /locus_tag="ACG8E6_002845"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5063163.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4506..5369
                     /locus_tag="ACG8E6_002846"
     CDS             4506..5369
                     /locus_tag="ACG8E6_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFS5063164.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHLNQILRFELLRMMSWKVGIKTEFSLSVGKNYKYINKYID
                     EDLWNRLLSTYRMDSYENIWKSLFICHQLFREVSKEVAELLGFDYPEYGKNITRYTED
                     MYKKYVENDYF"
     gene            5413..5940
                     /locus_tag="ACG8E6_002847"
     CDS             5413..5940
                     /locus_tag="ACG8E6_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HFS5063165.1"
                     /translation="MESTDVINEIRGILPRNSIGKYDLLTIFTHKTVFDKIVKVLSLD
                     FQNKVDYVAAPEAIGWILGTAIAKELGVGFIGVRKGDKLPYAKEEIISTHFTDYSGQD
                     KSFEISKSSIVRNKRVLIVDDWIETGSQMKALIALLEKLDCSIIGLATIGIDINEVTQ
                     KWIDSSFVAYIGSNI"
     gene            6168..7487
                     /locus_tag="ACG8E6_002848"
     CDS             6168..7487
                     /locus_tag="ACG8E6_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSsu5 family transposase"
                     /protein_id="HFS5063166.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGRLSSDSGLVLVKELMNTFGFSH
                     LAKQWLHITDHRAYFTHDNLAILEQLIMQLIAGYSADSSANLLRRDPVFQAVLGKKQL
                     ASQSSISRFLDRFTEENIDQLQALNQSLIDKARLIRNDTELIIDVDSTHSDTFGRQEQ
                     TDYNAHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKAFINPLLHHYKSTLPH
                     TEILVRGDSGFATPEVYESCEATESQYVIRLKSNRRLSQLAEHSVLYGDNQEWEEREV
                     QYFSLPYQAQSWSKPRRVCIRSVREGGELLFHHAFIVTNLSDNVSPEVIFSLYGKRGT
                     MENFIKEAKGGFYFDKTDSPRFLENHVRMMISVLAYNLVNFLKSIGFDKVNQGMTIHS
                     IRLILLKVAGKLVQTGRQVYLKLSSYHVYQTEFYKVFERLRRSKQWI"
     gene            7639..9165
                     /locus_tag="ACG8E6_002849"
     CDS             7639..9165
                     /locus_tag="ACG8E6_002849"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFS5063167.1"
                     /translation="MAICSKKQVKFRAMNYSGFFEVEASIKVNQGSFKGVLSPTQRFK
                     SQEELLSFVSSKQAKIGNQEGIIADVQTKEKKTNSPSLFSLSSLQSKVNQLYKATASQ
                     TLKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMVQTEPRKRY
                     VDGSKVQEHHAIIPTKQVPTESALAKMDDLQRKIYALVVKTTVAMFLPDYLYEETKIQ
                     TKVADLLFQSIGKTPKQEGWKILFKQQTKEEKEDVQTLPLVIIGEHAEVDVKSAEKET
                     QPPKAFTEGTLLTAMKTANKTVDDEEAIKILQEVEGIGTEATRASIIEALKQKEYIQV
                     IKNKLVVTEKGKLLCQAVESQHLLTSAEMTAKWETYLKKIGKREGNQENFINNIKKFI
                     VHLLEAVPNDIEKLNFSDYQEQKEKEAEKSIVGKCPKCGNNIVLKKSFYGCSNYPECK
                     FTLAEHFRKKKLTKTNVKELLEGKETLVKGIKNKEKKPYNAVVKIGEKGYIDFISFSK
                     "
     gene            9285..9524
                     /locus_tag="ACG8E6_002850"
     CDS             9285..9524
                     /locus_tag="ACG8E6_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFS5063168.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDMMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            9573..9656
                     /locus_tag="ACG8E6_002851"
     CDS             9573..9656
                     /locus_tag="ACG8E6_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFS5063169.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            9781..10125
                     /locus_tag="ACG8E6_002852"
                     /pseudo
     CDS             9781..10125
                     /locus_tag="ACG8E6_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
BASE COUNT         3601 a         1611 c         1980 g         2974 t
ORIGIN      
        1 tctcaatcat ttagaattaa ttggaactcc agtttatggg gcaataatca aaatggacat
       61 tttgagaacc ccgttagctg acgatagtca taaagaaata gccagaggtt ttgaccgact
      121 aaagcaattt caagctttct ctactgaaaa atggaagatg gaatctattc ctgaagggaa
      181 aataaaagtt cttgcaaatt atgcatttaa agctaaagct caattaattc aacgaatgag
      241 tgaacagaaa aaactggcgt tgttagtggc atttattttc atatacaaac gtaaggctat
      301 ggatgaacaa attttagcac tatctaattt ttttgaaact gtttttagac gcgccaaaaa
      361 taaggaatct aaagaacgtc ttcgtaccat aaaagatcta gatcgagccg catcgacact
      421 ttcagatatt gtagatttag taatgtccag tcattctgat actaatgaac tatcccttcg
      481 cgatcgtatc ttagaaaaat attctgagga aaatgttacc gaagctgtat cgcaagttcg
      541 gctattggtt aaaaatgatc gtgaaccaat agccatttct gaacttctga acacttatcg
      601 aaagttcaga aaatttattc cggatatttt aactacacta acatttgaca gcaatgacta
      661 cggtaacaac tgtaaggaac tctggacctt cattaataat agatttccta agccgattac
      721 ttacaaaatg tttggagcta ttaaacatac tcttccaaag aaatggattt attacattca
      781 tgaataccct gaacaaacta ataaatgtgt attgattgca gctgtcgaat tgttaattca
      841 atctttaaaa cgccatgata tttatattcc tcagagtgtc ttatataaca atcctatgga
      901 ttgcttgatt gatgagaaaa cttgggttca acaaaaatac gttttattag aacaactaga
      961 tcttccttct tcagcaaatg aaatgtcttt atggttaaag aacgatttgg aactgtctta
     1021 taaagaagcg ctaaatcgtt ggcctacttc taaaatggcc agaatagaaa atgcagatcg
     1081 cctggttgtc tctagactta aaaagacata tgaaaaaaag gaagataagt cttttagacg
     1141 tcgcattcag cagttacttc ctaaaattga tttgtcagat ttgttattgg aagtaaacca
     1201 acagttaaat cttactcaat catttaacca cttaagtgac aaggacacca aaatgaaaga
     1261 cttggatatt agtttattag cagttctttt atcagagtcc tgcaatattg gtttttcccc
     1321 ggtttcaaaa gaaggatttg attctttaaa gtatgatcgt cttgtctatg tcaatcatca
     1381 ttatattcga gtggatacac tttcaggagc taatcaaaaa ataatacatg cacacaggaa
     1441 acttgccacc tctaagatat ggggtgatgg gcatatggct tcagctgacg gtattcgtta
     1501 tatcacacca caacgttccc tatattctag aagtaatcca aaatattttg gccgtggtcg
     1561 tgggatcaca ttctacaatt ttgtctctga tcaatatata gggttccacg gaatggtcgt
     1621 ttcagggaca ctgcgagatt cgctttacct tctagaagga ttcctcaatc aacccagcga
     1681 attaactcct aatcagatca tgactgatac agctggttat agcgatatga tttttggtct
     1741 ctttggctta ttagggtttc aatttagtcc aagaattgcc aatgataaag gaaccaaact
     1801 ttggcgaatc gacgctaacg ctcactacgg ggaacttgat acgttgtctc aaaattcaat
     1861 taatatggaa ctgatttata cccattggga ggacattctt agggttgcag gttcgttgaa
     1921 atctggaaaa gtgaatgcaa cagaattaac gaaatctctt caacggaatg ggcaacccac
     1981 gcctttagga aaagcaataa ctgagtatgg aaaagtttat aagacgaaac atcagcttcg
     2041 ttatatttct gacgaaatat atgcacggca aattttagaa caattgaata agggggaagc
     2101 tcggcatgcc ttatgccgta atatttttta tggacgaaaa ggaaaactct accagactta
     2161 ttatgatggg atggaagagc agctcaatgc attaggatta gttactaatg ccatcatata
     2221 ttggaactca ctgtatttag aaaaagtatt tgaacaaatg aaagctgaag gattcgactg
     2281 ttccgaagaa atgatacgca ggcaatctcc actaattagt gatcacatca attttgttgg
     2341 taagtatacg tttagatacg aagctgaatt atccaacggt aagttacgtc cattgaatgt
     2401 agaagaattc tagcaatgtt cgggttttgc atgctaccgc actatttcgt atgactgttc
     2461 cagcgaatca cttcgaatag aaaaagagaa aaacacgaga acagcgttaa atcaacgttt
     2521 cttgtgtttt tacttttgtg ctaccgcact atttcgtttc tatgttccat gtaccccata
     2581 ttttaataac tattgtgttt atcaaccaaa tggtttataa tattcttagc gtaaacttgg
     2641 aggtgctgta tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt
     2701 gtttccgaac ttgctagacg tattggccag actccacaga atttcaataa aaaattacaa
     2761 cgagaaacag taaccttgga tgagttgaag gccattgctg atgtgctagg tgtcaagttt
     2821 gtgcaggcat ttattttacc agatggagaa gaaataaaaa tatctaacga ataaaggagg
     2881 atgtttatat ggtaggtaat gttattcaaa tagtaacaga gaaattatca tctttgcctt
     2941 ttatagaagg catcgtatta gggggctcac gtgcaagagg cacccataca gaaaattctg
     3001 atatagatat cggaatctat tacaattcag actcatttga cctgacagcg attaatcaaa
     3061 ttgctacaga attggatgat aaaaatagaa acaaccttgt tgtgcctccc ggagcgtggg
     3121 gtgattggat taatggcggc ggatggttat ttataaatgg gtatcatgtt gacttgattt
     3181 tacgtgatat aaaacgggta gaacaaataa tcaaagatac ggagcaagga attgttactg
     3241 ccaattatca gaccgggcat ccccatggtt atataagtgc aatgtatcgt ggagaattag
     3301 cgattagtaa aatactatat gctaagaacg aaagcttatg cgaattaaaa aagcaggcag
     3361 aaatttaccc tactgctcta aagaaaagct tgatgaactt ttttatattt gaagctgaat
     3421 tctctttaat gtttgtaaaa gcaaatgcgg gggtagagga taaatattat attgcaggcc
     3481 atgtttttcg tattatttca tgcttaaatc aagtactatt tgcatgtaat aatgcttatt
     3541 gcattaacga aaagaaagct ataaaactgc ttgaaacttt tgaatataaa cctgaaaaat
     3601 atgccgaaag agttaatcat atttttgaag tactcggtct ttctcttttt gaatgctacg
     3661 atatgaccga gaaactttat aaagaagtga atgaaattgt atcggagata aataactttt
     3721 taaacgagga gagttcagat gaaagaaaac aaatatgatg ataatatatt ttttcaaaaa
     3781 tacagtcaaa tgagtcgctc gcagaaagga ctggctggtg cgggagaatg ggagactttg
     3841 aaaaagatgc tacctgattt taagggtaag cgtgtgcttg atttaggatg cggctatgga
     3901 tggcactgta tatatgcgat ggaaaacggt gcttcctctg tagtaggtgt tgatatttct
     3961 cataaaatgc tcgaagtagc aaaaggaaaa acccattttc cacagattga atatgaatgc
     4021 tgtgccatag aagatgtgga tttcccagag gagagctttg atgtaatact aagttcgctt
     4081 gcgtttcatt atgtagcaga ctatgagaat ttaataaaaa agatatatag gatgctgaag
     4141 gctggtggca atttagtttt tacagttgaa catcctgttt ttactgctca tggaacacaa
     4201 gactggtatt ataacgaaaa aggagaaata ctgcatttcc cggtggacaa ttattattat
     4261 gagggcaaac ggacagctat gtttttggaa gaaaaggtta caaaatatca tagaacactg
     4321 accacatatc taaatacact gctttcaaat agttttataa taaatcagat tgtggagcca
     4381 cagccgccag agaacatgat ggatattccg gggatggcgg atgaaatgcg acgcccaatg
     4441 atgctgattg tatcggcaaa aaagaagatg taataatata gaaaaaataa acgaggagta
     4501 tgtaaatgag atcagaaaaa gaaatgatgg atttagtact ttctttagca gaacaggatg
     4561 aacgtattcg aattgtgacc cttgaggggt cacgcgcaaa tattaatata cctaaagatg
     4621 aatttcagga ttatgatatt acatattttg taagtgatat agaaccgttt atatctaatg
     4681 atgactggct taatcaattt gggaatataa taatgatgca aaagccggag gatatggaat
     4741 tattcccacc tgaagaaaag ggattttcct atcttatgct atttgatgat tacaataaaa
     4801 ttgatcttac cttattgccc ttggaagagt tagataatta cctaaagggc gataaattaa
     4861 taaaggttct aattgataaa gattgtagaa ttaaaaggga catagttccg actgatatag
     4921 attatcatgt aagaaagcca agcgcaaggg agtatgatga ttgctgcaat gaattttgga
     4981 atgtaacacc ttatgttatt aaaggattgt gccgcaaaga gatactgttt gcaatcgatc
     5041 atctgaacca gattctacgg tttgaactac ttaggatgat gtcgtggaag gttgggataa
     5101 agacagaatt ttcattaagt gttgggaaaa attataagta tattaacaaa tacattgatg
     5161 aagatctatg gaatagatta ttatctacat atcgcatgga ttcctatgaa aatatttgga
     5221 agtcattatt tatatgccac caattgttca gggaagtgtc caaagaggta gcagaactac
     5281 tggggtttga ttatccagag tatggtaaga acataacaag atataccgag gacatgtata
     5341 aaaaatatgt tgaaaatgac tatttttaaa gattagcaaa gattattaac cataagcaag
     5401 gggtgtaaat gtatggaaag tactgatgtt ataaatgaaa ttagaggtat attacctcgt
     5461 aactcaattg gaaaatatga tttactaact atttttacac ataaaaccgt ttttgataaa
     5521 attgtaaaag tactatcatt ggattttcaa aataaagtag attatgtggc tgccccagaa
     5581 gcaattggat ggatactagg cactgccata gccaaagaac ttggagtggg gtttattgga
     5641 gtaagaaaag gtgataagct gccatacgca aaagaagaga ttatttcgac acattttacc
     5701 gactattcag gtcaagataa aagctttgaa atatctaaga gttctattgt gaggaacaaa
     5761 agagttttga ttgtagatga ttggatagag acaggttctc aaatgaaggc tttaatagct
     5821 ttgttagaga aattggattg cagcattatt gggttggcta ctattgggat tgatataaat
     5881 gaggttactc aaaaatggat agacagcagt tttgtagctt atattggctc aaacatataa
     5941 taaaccgaaa gaaaatcttg aatcaaaatt ttggatggcg ttaatttaca atacaacgaa
     6001 agatgtttac cctgaataat tcatactttt taattgaaac cgtctgaaac tgcctaacgg
     6061 cagtctgata attacttttt tagtttcacc ccttaagtga tgaaaaaaga accccattct
     6121 gctatggtaa aggtgacgaa accaaccata agaaaggagt tcttctcatg actagcttac
     6181 acaaaaatca agttaaattc aattcaaata tcactatttc acataccggt ggtcgtctat
     6241 caagtgattc ggggttagtc ctggttaaag aattaatgaa taccttcggc ttttcgcact
     6301 tagctaaaca atggctccat attacagatc atcgggctta ttttacccat gataacttag
     6361 cgatattgga acagcttatt atgcaattaa ttgctggtta ctcggcagac tcatcagcta
     6421 atctattaag acgggatcct gtctttcaag ctgtactcgg taaaaaacag ttggcatcac
     6481 aatcctcaat ctcacggttt ctggatcgtt tcaccgagga gaacatcgat caactccaag
     6541 cattaaatca gtcattgatt gataaagcac gtttaattcg caatgacacc gagttaatca
     6601 ttgatgtcga ttccacacat tcggatacct ttggacgcca agaacaaaca gattataatg
     6661 cccattatca aacctacggc tatcacccat tagttgcctt tgatggactg accggagatt
     6721 tcttaaaagc tgaactgcgt tcaggcaatc agtacacatc taaaggggta aaagccttta
     6781 tcaacccgct tttacaccac tacaagagca cgttacctca taccgagata ttggttcggg
     6841 gtgacagcgg cttcgccaca ccagaggtgt atgagtcttg tgaagcaact gaaagccagt
     6901 acgttatccg attaaagagc aatcggaggt taagtcagtt agctgaacat tctgttcttt
     6961 acggggacaa tcaagaatgg gaagagcgag aagtgcagta tttttcacta ccttatcaag
     7021 cacaatcctg gtcgaaacct cgtcgtgtct gtatacgctc cgtccgtgaa ggaggagaat
     7081 tgctatttca tcatgcgttt attgtgacta atctatccga caatgtctcg cctgaagtca
     7141 tattttctct ttacggcaag cgaggaacaa tggaaaattt catcaaagaa gcgaaaggtg
     7201 gcttttactt cgataaaaca gatagcccgc gtttcttaga aaatcatgtc agaatgatga
     7261 tcagtgtcct tgcatacaac ctcgttaatt ttttaaaaag cattggtttt gataaagtga
     7321 accagggcat gacgattcat tccatccggc tgatattgct taaagtagcc ggtaaacttg
     7381 ttcagactgg gagacaggtc tatctcaaac tgtcaagtta tcatgtgtat caaactgaat
     7441 tttataaggt ttttgaacgc ctgcggcgat ccaaacaatg gatttagact agttatcaca
     7501 caaatttttt aaccgattct ttttcaaggg attcgtctgc ccttaaatag acaaataatt
     7561 tttattatga tcttcttccg taggcatcct gttcgaaaac acattatttt ggaagaaagt
     7621 atctgcctaa tttaaaaatt ggcaatttgt tcaaaaaaac aggttaaatt tagagctatg
     7681 aattattcag gttttttcga ggtggaagct agtataaaag taaaccaagg gtcgtttaag
     7741 ggcgttctaa gccccacaca gcgttttaag agccaagagg aacttttatc ctttgtttct
     7801 tctaaacaag ctaaaatagg caatcaagag gggataattg ctgatgttca aaccaaagag
     7861 aagaaaacga atagtccgag tttgttttct ttaagtagtt tacaatcaaa agtcaatcag
     7921 ctttataaag cgacagcgag ccaaacttta aaagctatgc aaggactgta tgaagcaaaa
     7981 ttattgagtt atccaagaac agatacacca tttattacag agaacgaatt tgcttattta
     8041 aaagcaaatt ttggcaaata tagcggtttt ttaggacttg atctcgaaat ggttcaaaca
     8101 gagcctagaa agcgttatgt ggacggtagt aaggtacagg aacaccacgc cattatccca
     8161 acaaaacaag tacctaccga atctgcatta gcaaaaatgg acgatttaca acgaaaaata
     8221 tatgctttag tcgttaaaac gaccgttgcc atgtttttac ctgattactt gtatgaagaa
     8281 accaagatac aaacgaaagt agctgatttg ctgtttcaat cgatcggaaa gacaccaaaa
     8341 caagaaggtt ggaaaattct tttcaaacaa caaaccaaag aagaaaaaga ggacgttcaa
     8401 acgttaccct tggttatcat tggcgaacat gccgaggttg acgttaagag tgccgaaaaa
     8461 gaaacacaac caccgaaagc ttttacagag ggtacattat taactgctat gaaaaccgct
     8521 aataaaacgg ttgatgatga agaagcaatc aagattttac aagaagtcga ggggatcgga
     8581 acagaagcga caagagcaag tattattgaa gcgttaaaac aaaaagaata tatccaagtg
     8641 attaagaata agcttgttgt aactgaaaaa ggaaaattat tgtgccaggc agttgaaagt
     8701 cagcaccttt taacgagtgc tgaaatgacg gctaaatggg aaacgtattt aaaaaaaatc
     8761 ggtaaaagag aaggcaatca agaaaacttt attaataata tcaagaagtt tatcgttcat
     8821 ttactggaag ctgtacctaa cgatatagaa aaactaaatt tttctgatta ccaggaacag
     8881 aaagaaaaag aagctgaaaa aagtattgta ggaaaatgtc ctaagtgtgg caacaatatt
     8941 gtattaaaaa aatcgtttta tggttgttca aattatcctg aatgtaagtt tactttagct
     9001 gaacatttta gaaagaaaaa actaaccaaa acgaatgtaa aagaattact ggagggaaaa
     9061 gaaaccctgg taaaaggaat caaaaacaaa gagaaaaagc cctacaatgc cgttgtaaaa
     9121 atcggagaaa agggatatat tgattttatc tctttctcaa aataaacata aaagcccttt
     9181 aaacagggct tttatatatt aatcacaaat cacctatcac aaatcacaaa tcacttgcga
     9241 tttgtgatga taaaataaga gtaagaagaa atagaaagaa gtgagtgatt gtgggaaatt
     9301 taggcgcaca aaaagcaaaa cgaaatgata caccaatcag tgcaaaaaaa gatatgatgg
     9361 gagataagac ggttcgtgtt cgtgctgact tgcaccatat cataaaaatc gaaacagcaa
     9421 agaatggcgg aaacgtaaaa gaagttatgg aaataagact tagaagcaaa cttaagagtg
     9481 tgttgatagt gcattatctt aaaattttgt ataataggaa ttgaagttaa attagatgct
     9541 aaaaatttgt aattaagaag gagggattcg tcatgttggt attccaaatg cgtaatgtag
     9601 ataaaacatc tactgttttg aaacagacta aaaacagtga ttacgcagat aaataaatac
     9661 gttagattaa ttcctaccag tgactaatct tatgactttt taaacagata actaaaatta
     9721 caaacaaatc gtttaacttc tgtatttatt tatagatgta atcacttcag gagtgattac
     9781 atgaacaaaa atataaaata ttctcaaaac tttttaacga gtgaaaaagt actcaaccaa
     9841 ataataaaac aattgaattt aaaagaaacc gataccgttt acgaaattgg aacaggtaaa
     9901 gggcatttaa cgacgaaact ggctaaaata agtaaacagg taacgtctat tgaattagac
     9961 agtcatctat tcaacttatc gtcagaaaaa ttaaaactga acattcgtgt cactttaatt
    10021 caccaagata ttctacagtt tcaattccct aacaaacaga ggtataaaat tgttgggaat
    10081 attccttacc atttaagcac acaaattatt aaaaaagtgg tttttggata aagtccgtat
    10141 aattgtgtaa aagtaaaaag gccata
//
LOCUS       DAZUMM010000248         6853 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.guided.111, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000248 DAZUMM010000000
VERSION     DAZUMM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.guided.111"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1168
                     /locus_tag="ACG8E6_002853"
     CDS             14..1168
                     /locus_tag="ACG8E6_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFS5063170.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            1383..2351
                     /locus_tag="ACG8E6_002854"
     CDS             1383..2351
                     /locus_tag="ACG8E6_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFS5063171.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            2344..3375
                     /gene="vanA"
                     /locus_tag="ACG8E6_002855"
     CDS             2344..3375
                     /gene="vanA"
                     /locus_tag="ACG8E6_002855"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFS5063172.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            3381..3989
                     /locus_tag="ACG8E6_002856"
     CDS             3381..3989
                     /locus_tag="ACG8E6_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFS5063173.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            4417..5328
                     /gene="vanY"
                     /locus_tag="ACG8E6_002857"
     CDS             4417..5328
                     /gene="vanY"
                     /locus_tag="ACG8E6_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFS5063174.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYETWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            5481..5966
                     /gene="vanZ-A"
                     /locus_tag="ACG8E6_002858"
     CDS             5481..5966
                     /gene="vanZ-A"
                     /locus_tag="ACG8E6_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFS5063175.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            6280..>6853
                     /locus_tag="ACG8E6_002859"
     CDS             6280..>6853
                     /locus_tag="ACG8E6_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HFS5063176.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKR"
BASE COUNT         2208 a         1183 c         1504 g         1958 t
ORIGIN      
        1 acggtatggg gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga
       61 acgaaaactt tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta
      121 tattcgttca atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa
      181 atatgactta aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat
      241 agatatcttt atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct
      301 ttcaaaattc gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa
      361 cgaagataaa caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac
      421 attaaaacgg actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa
      481 tgacgttgtt atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta
      541 tttgagcctg cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca
      601 tatcacgttg gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac
      661 acggtataac ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct
      721 ggtgcagatg accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat
      781 tcacgccccc gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa
      841 caacattttg aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc
      901 gggcctctcc ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga
      961 taagctagct gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac
     1021 gggtggcgcg ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca
     1081 gatttacgcg gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc
     1141 agacttggtt gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa
     1201 ggttatcttt actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt
     1261 acacggtaga cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac
     1321 aagattacca ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa
     1381 ttatgaataa catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc
     1441 atgctctttc gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat
     1501 ccaacgccaa atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt
     1561 ccgcctctat tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca
     1621 tcggctgcaa tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg
     1681 tggcgtactc gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac
     1741 gcaacgtaaa atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc
     1801 gtggcaaggt actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag
     1861 cggttattga gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa
     1921 gtatagaggt aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc
     1981 ttcatgtgcc gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa
     2041 tgaagcaagg agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt
     2101 tggttaaagc attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag
     2161 aggaagagtt tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta
     2221 aacttcaaag aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag
     2281 cgttgcgtga taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg
     2341 agcatgaata gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta
     2401 tcggtaaaat ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta
     2461 tacattggaa ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg
     2521 gaaaacgaca attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt
     2581 gttaaaaaga accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat
     2641 ggcaagtcag gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt
     2701 gtaggctgcg atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt
     2761 gcgaaaaatg ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg
     2821 gtggcagcta cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc
     2881 ggtgtgaaaa aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa
     2941 tatgacagca aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta
     3001 ttgggaaaca gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga
     3061 atctttcgta ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc
     3121 gttcccgcag acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata
     3181 tataaagcgc tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc
     3241 cgcattgtac tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc
     3301 cgtatgatgg ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta
     3361 gcgttaaagg ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg
     3421 gtgttcgttg ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg
     3481 gttatgaagt aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa
     3541 aagaactggc tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc
     3601 gtgctgtaaa ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa
     3661 gttattatcc caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat
     3721 caagccatag ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc
     3781 ttgtaccaat ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg
     3841 gaatatcatg caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg
     3901 ggtttgaagc atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca
     3961 atagctattt tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaactatata
     4021 agctaactct ttcggcagga aacccgacgt atgtaactgg ttcttaggga atttatatat
     4081 agtagatagt attgaagatg taaggcagag cgatattgcg gtcattatct gcgtgcgctg
     4141 cggcaagata gcctgataat aagactgatc gcatagaggg gtggtatttc acaccgccca
     4201 ttgtcaacag gcagttcagc ctcgttaaat tcagcatggg tatcacttat gaaaattcat
     4261 ctacattggt gataatagta aatccagtag ggcgaaataa ttgactgtaa tttacggggc
     4321 aaaacggcac aatctcaaac gagattgtgc cgtttaaggg gaagattcta gaaatatttc
     4381 atacttccaa ctatatagtt aaggaggaga ctgaaaatga agaagttgtt ttttttattg
     4441 ttattgttat tcttaatata cttaggttat gactacgtta atgaagcact gttttctcag
     4501 gaaaaagtcg aatttcaaaa ttatgatcaa aatcccaaag aacatttaga aaatagtggg
     4561 acttctgaaa atacccaaga gaaaacaatt acagaagaac aggtttatca aggaaatctg
     4621 ctattaatca atagtaaata tcctgttcgc caagaaagtg tgaagtcaga tatcgtgaat
     4681 ttatctaaac atgacgaatt aataaatgga tacgggttgc ttgatagtaa tatttatatg
     4741 tcaaaagaaa tagcacaaaa attttcagag atggtcaatg atgctgtaaa gggtggcgtt
     4801 agtcatttta ttattaatag tggctatcga gactttgatg agcaaagtgt gctttaccaa
     4861 gaaatggggg ctgagtatgc cttaccagca ggttatagtg agcataattc aggtttatca
     4921 ctagatgtag gatcaagctt gacgaaaatg gaacgagccc ctgaaggaaa gtggatagaa
     4981 gaaaatgctt ggaaatacgg gttcatttta cgttatccag aggacaaaac agagttaaca
     5041 ggaattcaat atgaaacatg gcatattcgc tatgttggtt taccacatag tgcgattatg
     5101 aaagaaaaga atttcgttct cgaggaatat atggattacc taaaagaaga aaaaaccatt
     5161 tctgttagtg taaatgggga aaaatatgag atcttttatt atcctgttac taaaaatacc
     5221 accattcatg tgccgactaa tcttcgttat gagatatcag gaaacaatat agacggtgta
     5281 attgtgacag tgtttcccgg atcaacacat actaattcaa ggaggtaagg atggcggaat
     5341 gaaaccaacg aaattaatga acagcattat tgtactagca cttttggggt aacgttagct
     5401 ttttaattta aaacccacgt taactaggac attgctatac taatgataca acttaaacaa
     5461 aagaattaga ggaaattata ttgggaaaaa tattatctag aggattgcta gctttatatt
     5521 tagtgacact aatctggtta gtgttattca aattacaata caatatttta tcagtattta
     5581 attatcatca aagaagtctt aacttgactc catttactgc tactgggaat ttcagagaga
     5641 tgatagataa tgttataatc tttattccat ttggcttgct tttgaatgtc aattttaaag
     5701 aaatcggatt tttacctaag tttgcttttg tactggtttt aagtcttact tttgaaataa
     5761 ttcaatttat cttcgctatt ggagcgacag acataacaga tgtaattaca aatactgttg
     5821 gaggctttct tggactgaaa ttatatggtt taagcaataa gcatatgaat caaaaaaaat
     5881 tagacagagt tattattttt gtaggtatac ttttgctcgt attattgctc gtttaccgta
     5941 cccatttaag aataaattac gtgtaagatg tctaaatcaa gcaatctgat ctttcataca
     6001 cataaagata ttgaatgaat tggattagat ggaaaacggg atgtggggaa actcgcccgt
     6061 aggtgtgaag tgaggggaaa accggtgata aagtaaaaag cttacctaac actatagtaa
     6121 caaagaaagc ccaattatca attttagtgc tgaggaattg gtctctttaa taaatttcct
     6181 taacgttgta aatccgcatt ttcctgacgg tacccctatc atagcatttt taaaaataaa
     6241 ttttatgtag acttaaaatt tacttcatgg agggtttaaa tggatgaaat caaaagtaac
     6301 aaaaaagcat ttggcattta tttaggagaa cctagtacag aacaattaaa ccaatttttt
     6361 tatttgcatg aaaaagattt agaattgatg gagactatac gacgaccagc tactagatta
     6421 ggttttgcac tacagttagt ctcactacgt tttttaggaa cttttatatt agatcttacc
     6481 aatattcccc aaaatgtatt gagttatgta gcaactcaac taaatattga tcctggagaa
     6541 attatctact atagcaggag acagaccagg tatcagcaca caaaattgat aaaatcacat
     6601 tatggatatc aagatttcaa ccaaccggaa atagaaacat acctagctaa ctggctttta
     6661 aatagagcgc tgtatacaac agaatcttca gatatgttgt ttgatatgct tctaaaaaaa
     6721 tgtctggatg aaaagattgt tttacctgga attactactt tttctcgttt tgttgctagt
     6781 gcaattgaaa aagctgaaga gcatttatac cgacaattgt ctttggttcc aactgaagat
     6841 gagagaaagc ggc
//
LOCUS       DAZUMM010000249         5596 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.guided.118, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000249 DAZUMM010000000
VERSION     DAZUMM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.guided.118"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..580
                     /locus_tag="ACG8E6_002860"
     CDS             116..580
                     /locus_tag="ACG8E6_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5063177.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            564..1193
                     /locus_tag="ACG8E6_002861"
     CDS             564..1193
                     /locus_tag="ACG8E6_002861"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS5063178.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            1547..3025
                     /gene="msr(C)"
                     /locus_tag="ACG8E6_002862"
     CDS             1547..3025
                     /gene="msr(C)"
                     /locus_tag="ACG8E6_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS5063179.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3072..3518
                     /locus_tag="ACG8E6_002863"
     CDS             3072..3518
                     /locus_tag="ACG8E6_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS5063180.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3717..4232)
                     /locus_tag="ACG8E6_002864"
     CDS             complement(3717..4232)
                     /locus_tag="ACG8E6_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5063181.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4349..5550
                     /locus_tag="ACG8E6_002865"
                     /pseudo
     CDS             4349..5550
                     /locus_tag="ACG8E6_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         1738 a          935 c         1198 g         1725 t
ORIGIN      
        1 tttctattga gagcgttttt tttattacac agctgattct ttgacttttt tcttttttta
       61 ttctattatt gacaagtcaa tcgttgacga gtcaattatt agaaaggagg aaattatgtc
      121 tttagaagag attagtcacc ttttatatca aatcaaatta gccgatcaaa aaatcgtcca
      181 gatctttgaa gaaaatatcg gtataagtct tactagatac gagatcatga tgagactgaa
      241 agaagaaaat ccattgctac aaagcagttt gcaagaaaag ttgcagattg atcaaggagc
      301 agttactcgt catctgaagg tattggaagc aaatggctat gtttcgcgtc agagaaatcc
      361 tcagaacaac cgggaagtgt atgtagcgct aacagaaaaa gcccagaaga aaatcgagca
      421 ttgtgaaatg gaccataaag atacaggttc cgtgctatcc ccggctttca gcgatgtaga
      481 aatgaaacaa tttctaacac ttttagataa attgaatacc caactttgca atataacaac
      541 agaaacgaaa gaggttaata ataatggaac aaaaaaatga tttttcaaca attatgactg
      601 gtagaagatc cgttcgcgta tatgatgaaa catacaaaat cccacacgaa gaaatgctgg
      661 aaatgatcaa agaagcgacg actgcacctt cttctgtcaa tatgcaacca tggcgctttg
      721 tggtcgctga aagcgaggaa gcaaaagaaa tcttgcgtcc tttgatccgt ttcaatacga
      781 gacaaaatga tacttcttct gcaatggtca tgatttttgg cgatatgcaa tgttacgaat
      841 atggcgaaga aatctataac caagcttacg aatcagggaa gatgccaaaa gaagtcagag
      901 atcaacaatt agctgcgatc attccttatt acaagagttt ttcaagagaa gaaatgaacg
      961 atgtagtcaa agtcgattct agcttagcgg ctatgcaatt catgttagtt gcgcgtgatc
     1021 atggctatga aacaaatcca attggtggat tcgaagcaga tcagttggca gaggcatttg
     1081 gtttggataa agaccgttat gttccagtta ttattttatc aataggaaaa gcggtagaag
     1141 aaggttacga gtctgttcgt ttagcaccag aaaagatcac tacgttcgaa taaaaggttt
     1201 tcagttacta ttcagtagct attgcttttt ttgaatcgtg tgtggtaacc taataaaaaa
     1261 ggaggtcgga aaaatgactg catcgatgaa attacgtttc gaacttttga ataacaacta
     1321 aataaattca aaagacatgt ataaacaaca atttcatgga tggagtatgt ccattttttc
     1381 gaacgacagt agaataggtg aaagctatga cagatttggt gatttagtca tagttatttc
     1441 gttcagagta ggaaaagatg tgatgcagtt tttttgcaaa cagatttttc tgctcttttt
     1501 tagtatcgat aattgttgtt ttacatgaaa taggagtgta aaacatatgg aaaatttagc
     1561 agtaaatata acaaatctac aagtcagctt tggcaaccag ctagaactat ctattgattc
     1621 tcttcgtgtc tatcagcaag atcgtatagg gatcatagga gaaaatggtg tcggtaaatc
     1681 gactttgctc aaactaatag ccggtgaact ttttcccgat catgggaaaa tccaaacaga
     1741 gatcaccttc aactacctgc ctcaattaac ctatctcgct gaagcaaagg acctaaattt
     1801 ggaattagcc agtcatttcc agttaagact ggaagaaact tcagagcgga aatggagcgg
     1861 aggggaagaa cgaaagatcg agttgatacg tcttctttct tcttatgaac aagggatgct
     1921 tctagacgag ccaacaaccc atctagatag aaaaagtatt gatcgactga ttgaagagct
     1981 tcgttattat tatggtacgc tggtttttgt tagtcatgat cgctattttc tagatgagct
     2041 agcatcgaaa atctgggaag taaaagacgg agaaatccga gagttttcgg ggaattatag
     2101 tgcctatctc actcaaaagg aattggagaa aaagactcag ctacgagaag cagagtcgat
     2161 catgaaagag aaaaaacgat tggaaaaatc gatccaagaa aagaaaaaac aagcggaaaa
     2221 gttggaaaaa gtgtccagta aaaagaaaaa gcaacaaatc agaccggatc ggttgtcttc
     2281 ctctaaacaa aaagacagtg tacaaaaagc catccaaaag aatgcgaaaa cattagagag
     2341 aagactccaa aaaataggag aaacaaccaa gccgcagcag atgaaacaaa tccgttttcc
     2401 agtaccaaaa tctcttgaac tccacagccg ctatcccatc atgggacaaa atgtccaatt
     2461 ggaaagaagt gggagaacat tactggtaaa tggtgatttt cagttttctt taggtaaaaa
     2521 aatcgcgatt gtcggtgaaa atggttcagg taagacaacc ttattagaac atatccgcaa
     2581 acaaggagaa ggaatccttc tctctccgaa agtaagcttt caagtatatc agcaaaaggg
     2641 ttatcaaatg acatctgaag aatccatcat tcgttttgtc atgagacaaa cagagttttc
     2701 agaatcgctt gtccgtagtt tgctgaatca cttagggttt gctcaggaaa ctttgacgaa
     2761 accgttatgt acattaagtg ggggagaagc gacccgtctg acgattgctt tgctttttac
     2821 taagccaagt aatgtgttgc tgttagatga accgactaat tttattgata tggcaacgat
     2881 cgaagcttta gagaagctaa tgcaaatata tccgggaacg attttgttta cttctcatga
     2941 ttcttacttt gtcgagcgta cggctgatga agtttatgaa ataaaagggc agaaaataaa
     3001 aaaagtactt acgagaaatt tttaaatgtg ctacgatgat tttcgtgaca aatttaaagg
     3061 agtggtacag attgcctaca aataaaaaag aaggaataat ttttacaaca atgatgtgtt
     3121 ttttgatggt cttggggatg agcgcgtata atttatggct gcatcaagat cttcatttat
     3181 caagtttgtt gattggtcta gtgcctggat tcgtggtggc tttcattttg gatgtcttca
     3241 tcatcggtgt gctggcgaaa aaaattgcct ttaaactacc agtaaatcca gaaagcaagg
     3301 tccaaatgat cttaactatt tcatgcttga tggttttagg aatggtgaca tgtatgtctt
     3361 tattcggtgt gatcatggaa caaggaatac ctgatcaatt attctctgct tatatgaatg
     3421 cttggaaaat gaatgtgatt gccgctttac cgttgcagtt gatcatcgtt ggcccgcttt
     3481 caagaagtgt gctaaaagca attcaaagta atgaatgatg gaacactgtt atttgaaaaa
     3541 aggccttggc atgctttttg tcatagtctc ttgaatgcag ttctagaaaa atagtgatcc
     3601 gaacgatagg ttttggcctg tcgttcggat cgctttttct tttccaactg tattgctctt
     3661 ttattgaaga taatttgctc ttcaatagat atttctagaa aggttctgtt tatcccttac
     3721 tcaaaaaact gctgcatttg ttctcttact tgctgacacc attggagttc ctgctgacaa
     3781 agcataattc tgcgctggat cacaacataa cgtccaaatt cttgtttctt gtctagcgag
     3841 aggtcattca attttttttg atttttttgc atgattttgt tcagctgttg ttctctagtg
     3901 aatagcagtc cagtagctgt tgttttatcg agcgtcgaaa aagcatagat ttttgctaga
     3961 aattcatctt tttgcgtagg agcgggagtt tcctctttga tccattcctc tacaagagca
     4021 cgtccttcat cagtcaagtg gtagatcttt ttttgagagt gctcgtcttt gtccaaaacg
     4081 gtcacataac cctgctggtc taacttggca agcgttgtat agatctgact gtgatgggct
     4141 tcccaaaaaa ttgtgatcag ctgcttcaat tcatacccag aacgtggttt ggataaaagc
     4201 acgcataata atatataggc taaagtattc ataaaaatcc tacctctcgt cttattttct
     4261 ttattatatc acagtaaagt ttccacgttt tacatgtgca tatttacatg taaaataaat
     4321 catgtatgat gctaatagag agaaagggat ggaaatattg gagaatcaaa tagatataca
     4381 agaacaaaca aatgaagaaa caaaggggaa aagttcagcg ctagttgtag cagctacctg
     4441 tatgatcgct tttatgggag tgggtctagt agacccaatc ttaaaaacga ttgctgcaca
     4501 gttaaacgct acgccagctg aaacgaccct gttatttact agttacatgt tagtcactgg
     4561 tgtggtgatg ctatttacag gtttcttatc tagtcggatc ggtttgaaaa aacattgatg
     4621 acaggattgt ttatcatcgt tgtattcgct ggtcttggcg gattatctgg gaatatcgga
     4681 atgctgattg ggttacgtgc cggctggggt gtcgggaatg ctttatttgt ctctactgct
     4741 ttagcaacga tcgtcagtat tttagttggt aacacagaaa aagcaatcat gatgtatgaa
     4801 gcagcacttg gctgcggaat ggctgttggc cctctagtcg gtggggtctt gggcagtggt
     4861 tcatggcgtt atccattttt tggggtagca tttttgatgt tttgcggttt tatagcccta
     4921 gcggtacttt tacctgaaac tgagaaacca aagagaaaag taggcctttg ggaaggtgtc
     4981 aaagcgttaa gcaatcataa acttcgttca gttggactaa tcgcattgtt atataatttc
     5041 ggattcttta ctttacttgc gtatgcgcca tttttattga ttggctattc tgaattagaa
     5101 gtcggttttg tattttttgg ctggggtgtg cttctggcta ttgcttcgat tttcgttgca
     5161 ccggctcttg agcggaagtt tacgacttat cggacaattt tagcagcatt tgtcttattc
     5221 attatttgtt tgctgttatt aggagctgga acttctatgc cagtccttgt accgatcagc
     5281 attgtgttag caggattttt ccaaggaatt atcaatacac tgttgacgac gattgctatg
     5341 gaaataccag gtctgcaacg aaacgttgct tcgtccagct atagttttgt ccgctttttc
     5401 ggtggagctt tagcaccatt tattgcaggt gtgattggtg aacatattgg tgaaagctat
     5461 gcgttttatt ttgcaggtgc cattgttctt atcagtgtat gttgtatcat ttatcaccgt
     5521 aattattttg ctacaaatga agaagaataa gaaatcagag gttgtgaaaa agctgtcctg
     5581 catcaagtca taagaa
//
LOCUS       DAZUMM010000250         5405 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.guided.121, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000250 DAZUMM010000000
VERSION     DAZUMM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.guided.121"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..664)
                     /locus_tag="ACG8E6_002866"
                     /pseudo
     CDS             complement(59..664)
                     /locus_tag="ACG8E6_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(805..3771)
                     /locus_tag="ACG8E6_002867"
     CDS             complement(805..3771)
                     /locus_tag="ACG8E6_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFS5063182.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLQADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SEKVVSLNSLRPLKLS"
     gene            3917..4492
                     /locus_tag="ACG8E6_002868"
     CDS             3917..4492
                     /locus_tag="ACG8E6_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5063183.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            4706..5401
                     /gene="vanR-A"
                     /locus_tag="ACG8E6_002869"
     CDS             4706..5401
                     /gene="vanR-A"
                     /locus_tag="ACG8E6_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFS5063184.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
BASE COUNT         1650 a         1012 c          963 g         1780 t
ORIGIN      
        1 tacatggaac atagaaacga aatagtgcgg tagaaaagcg aatgaatgta gtatatttct
       61 acaaatcatt cgctttcttt attttataaa tagttggtcg agagatcccg ttttcttttg
      121 caatctctgc aactggaatg ccttgcttta acatgtccac cacggtttta aaaactaagc
      181 gtttttgcgg atctgttgag ttaatagaat actcgattgg tcgtccctta tatcggccgg
      241 ctttttgagc caataatact ccttaccgct gacgttcacg aattttctca cgctcgtttt
      301 gtgcaatata acttagcagt gataaaaaca tatcgaacat agcggtatcc aggagttcat
      361 ttccagtatt aaaatctaaa aactgtgcat ctaatatttt taacgctact tttttctgtt
      421 tcataaaagc aactgtattt tttatatctt cataatctct tcctaaacga tctagtgatg
      481 taacgatgac gcaatcacct tcacggatat aatttagtaa tttttgaaac tcaggacgct
      541 cagtatcttt accactggct ttttcttgga ataatttatc aatgttaagt ccttccatca
      601 ttgccaattg tcgatccaaa ttttgatctt gtgaagatac tcgaatatag ccaatttttc
      661 ccataaaaaa ctccttttta aaagtgatat aatttacatg taaaatttac ttgtagtttt
      721 actgctatca ggggtagcgt caggaaaatg cggatttaca acgctaagcc tattttcctg
      781 acgaatccct cgtttttaac aacgttaaga aagttttagt ggtcttaaag aatttaatga
      841 gactactttc tctgagttaa aatggtattc tcctagtaaa ttaatatgtt cccaacctaa
      901 gggcgacata tggtgtaaca aatcttcatt aaagctacct gtccgttttt tatattcaac
      961 tgctgttgtt aggtggagag tattccaaat acttatagca ttgataatta tgtttaaagc
     1021 actggctctt tgcaattgat gctgtatggt gcgttctcta agctcacctt gttttccgaa
     1081 gaaaatagct cttgccaatc cattcatggc ttctccttta ttcaatcctc tttgtatttt
     1141 tcttcttaat gattcatccg atatataatt caaaataaag atcgtttttt ctattcggcc
     1201 catctcacgt aaggctgtag ctaagctgtt ttgtcttgaa taggaaccta gcttccccat
     1261 aataagggat gctgaaactg ttccctccct tatagaatga gctaatcgca aaacatcctc
     1321 ataattttct ttaatgacct ttgtatttat ttgtccacgt aaaatggctt ctagttttgg
     1381 atactcactt gctttatcta tcgtaaataa ttttgagtcc gataaatccc ttattcttgg
     1441 ggcaaattta aatcctaata aatgagtcag tccgaatatt tggtcagtgt aaccggcagt
     1501 gtctgtataa tgttcctcta tgtttagatc cgtctcatga tgtaacaaac catccaaaac
     1561 atgaatcgca tctcttgaat tagtatgaat aatctttgtg tagtaagaag agaattgatc
     1621 acttgtaaat cggtagatgg tggctccttt tccagttcca taatgtggat ttgcatctgc
     1681 ttgtagtgat gaaacaccta gctgcattct cataccatct gacgaagatg ttgtaccgtc
     1741 gccccaatag aaaggcaatt gtaatttatg atgaaagttt actaatatgg cttgggcttt
     1801 attcatggca tcttcataca tgcgccattg agatacattg gctagttgct tatatgtaag
     1861 tccgggtgtg gcttcggcca tcttgctcaa gccaatattc attcccattc ctaaaagggc
     1921 agccatgata atgattgttt cttccttatc tggttttcga ttattggaag catgagtgaa
     1981 ttgctcatga aatcctgtta tatgggccac atccatgagt aaatcagtta attttattct
     2041 tggtagcatc tgataaaggc ttgcactaaa tttttttgct tcttctggaa catctttttc
     2101 taagcgtgca agtgatagct ttcctttttc aagagaaacc ccatctaact tattggaatt
     2161 ggcagctaac cactttaacc tttcattaaa gctgctggtt ctctccgtta tataatcttc
     2221 gaatgataaa ctaactgata atctcgtatt ccccttcgat tgattccatg tatcttccga
     2281 aaacaaatat tcctcaaaat ccctatattg tctgctgcca acaatggaaa catctcctgc
     2341 ccgaacatgc tcccgaagtt ctgttaaaac agccatttca tagtaatgac gattaattgt
     2401 tgtaccatca tcctcgtata aatgtctttt ccatcgtttt gaaataaaat ccacaggtga
     2461 gtcatcaggc acttttcgct ttccagattc gttcattcct cggataatct caacagcttg
     2521 taaaagtggc tcatttgcct ttgtagaatg aaattccaat actcttaata gcgttggcgt
     2581 atattttctt agtgaataaa accgtttttg cagtaagtct aaataatcat agtcggcagg
     2641 acgtgcaagt tcctgagcct cttctactga agagacaaag gtattccatt caataaccga
     2701 ttctaaaacc ttaaaaacgt ctaatttttc ctctcttgct ttaattaatg cttgtccgat
     2761 gttcgtaaag tgtataactt tctcatttag ctttttaccg ttttgtttct ggatttcctc
     2821 ttgagcctta cgaccttttg ataacaaact aagtatttgc ctatcatgaa tttcaaacgc
     2881 tttatccgtt agctcctgag taagttgtaa taaatagatg gttaatatcg aataacgttt
     2941 attttcttga aagtcacgga atgcatacgg ctcgtatctt gagcctaagc gagacagctg
     3001 caacaggcgg ttacggtgca aatgactaat ttgcactgtt tctaaatcca ttcctcgtat
     3061 gtattcgagt cgttctatta tttttagaaa agtttcgggt gaaggatgac ccggtggctc
     3121 ttttaaccaa cccaatatcg ttttattgga ttcggatgga tgctgcgagg taataatccc
     3181 ttcaagcttt tctttttgct catttgttag agatttacta accgtattaa atagcttctt
     3241 ttcagccatt gcccttgctt cccacaccat tctttcaagt gtagtgatag caggcagtat
     3301 aattttgttt tttcttagaa aatctatgca ttcatgcagt agatgaatgg catcaccatt
     3361 ttccaaagct aattgatgaa ggtacttaaa tgtcattcga tattcactca gggtaaaagt
     3421 tacaaagtcg tattcacttc gaatttcttt caaatgatcc caaagtgtat tttccctttg
     3481 aggataatga tcaagcgagg atggactaac accaatctgt ttcgatatat attgtatgac
     3541 cgaatctggg atgcttttga tatgagtgta tggccaaccg ggataccgaa gaacagctaa
     3601 ttgaacagca aatcctaaac ggttttcttc cctccttcgc ttattaacta tttctaaatc
     3661 ccgtttggaa aaagtgaagt aggtccccag tatccattca tcttcaggga tttgcataaa
     3721 agcctgtctc tgttccggtg taagcaattc tctacctctc gcaattttca ttcagtatca
     3781 ttccatttct gtattttcaa tttattagtt caattatata tcaatagagt gtactctatt
     3841 gatacaaatg tagtagactg ataaaatcat agttaagagc gtctcataag acttgtctca
     3901 aaaatgaggt gatattttgc ggaaaatcgg ttatattcgt gtcagttcga ctaaccagaa
     3961 tccttcaaga caatttcagc agttgaacga gatcggaatg gatattatat atgaagagaa
     4021 agtttcagga gcaacaaagg atcgcgagca acttcaaaaa gtgttagacg atttacagga
     4081 agatgacatc atttatgtta cagacttaac tcgaatcact cgtagtacac aagatctatt
     4141 tgaattaatc gataacatac gagataaaaa ggcaagttta aaatcactaa aagatacatg
     4201 gcttgattta tcagaagata atccatacag ccaattctta attactgtaa tggctggtgt
     4261 taaccaatta gagcgagatc ttattcggat gagacaacgt gaagggattg aattggctaa
     4321 gaaagaagga aagtttaaag gtcgattaaa gaagtatcat aaaaatcacg caggaatgaa
     4381 ttatgcggta aagctatata aagaaggaaa tatgactgta aatcaaattt gtgaaattac
     4441 taatgtatct agggcttcat tatacaggaa attatcagaa gtgaataatt agccattctg
     4501 tattccgcta atgggcaata tttttaaaga agaaaaggaa actataaaat attaacagcc
     4561 tcctagcgat gccgaaaagc cctttgataa aaaaagaatc atcatcttaa gaaattctta
     4621 gtcatttatt atgtaaatgc ttataaattc ggccctataa tctgataaat tattaagggc
     4681 aaacttatgt gaaagggtga taactatgag cgataaaata cttattgtgg atgatgaaca
     4741 tgaaattgcc gatttggttg aattatactt aaaaaacgag aattatacgg ttttcaaata
     4801 ctataccgcc aaagaagcat tggaatgtat agacaagtct gagattgacc ttgccatatt
     4861 ggacatcatg cttcccggca caagcggcct tactatctgt caaaaaataa gggacaagca
     4921 cacctatccg attatcatgc tgaccgggaa agatacagag gtagataaaa ttacagggtt
     4981 aacaatcggc gcggatgatt atataacgaa gccctttcgc ccactggagt taattgctcg
     5041 ggtaaaggcc cagttgcgcc gatacaaaaa attcagtgga gtaaaggagc agaacgaaaa
     5101 tgttatcgtc cactccggcc ttgtcattaa tgttaacacc catgagtgtt atctgaacga
     5161 gaagcagtta tcccttactc ccaccgagtt ttcaatactg cgaatcctct gtgaaaacaa
     5221 ggggaatgtg gttagctccg agctgctatt tcatgagata tggggcgacg aatatttcag
     5281 caagagcaac aacaccatca ccgtgcatat ccggcatttg cgcgaaaaaa tgaacgacac
     5341 cattgataat ccgaaatata taaaaacggt atggggggtt ggttataaaa ttgaaaaata
     5401 aaaaa
//
LOCUS       DAZUMM010000251         3864 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.guided.140, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000251 DAZUMM010000000
VERSION     DAZUMM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.guided.140"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8E6_002870"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8E6_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFS5063185.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="ACG8E6_002871"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="ACG8E6_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HFS5063186.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         1219 a          595 c          795 g         1255 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agag
//
LOCUS       DAZUMM010000252         3864 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.guided.141, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000252 DAZUMM010000000
VERSION     DAZUMM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.guided.141"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8E6_002872"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8E6_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFS5063187.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="ACG8E6_002873"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="ACG8E6_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HFS5063188.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         1218 a          595 c          796 g         1255 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agag
//
LOCUS       DAZUMM010000253         3756 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.guided.143, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000253 DAZUMM010000000
VERSION     DAZUMM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.guided.143"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..431)
                     /gene="dfr"
                     /locus_tag="ACG8E6_002874"
     CDS             complement(<1..431)
                     /gene="dfr"
                     /locus_tag="ACG8E6_002874"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DfrD/DfrG/DfrK family trimethoprim-resistant
                     dihydrofolate reductase"
                     /protein_id="HFS5063189.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKG"
     gene            706..1833
                     /locus_tag="ACG8E6_002875"
                     /pseudo
     CDS             706..1833
                     /locus_tag="ACG8E6_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
     gene            1931..3304
                     /gene="tet(L)"
                     /locus_tag="ACG8E6_002876"
     CDS             1931..3304
                     /gene="tet(L)"
                     /locus_tag="ACG8E6_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HFS5063190.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         1150 a          608 c          691 g         1307 t
ORIGIN      
        1 atcccttttt gggcaaatac ctcattccat tcctcataat tcacttctgg aaaaaaagta
       61 tctccttcga attcatgatg tatttttgtg atgtacattt tctcaacata agggaaaaac
      121 aaattataaa tctgttctcc tccgaaaata aaaatttctt cttcgttttt acataactca
      181 aaaacatctt ctattgaatg aacaatttca caaccattaa aggtaaaccc cttatctctc
      241 gtcagaataa tatttcttct gtcaggtaag gctcttccga ttgattcaag gttcttccta
      301 cctaatatta tcggatgtcc ctttgtagta tttttaacat attcccagtc cttgggaatc
      361 ctccaaggaa tgtcattctc tttgccaatc actctattct tatccatcgc agcaatcaaa
      421 gaaactttca tttataacac agccttttta actaatttac catctaattt cttatttcta
      481 tcaaaaaacc ttttctcatt atcctgcccg ttagttgaat aagcaatttt ttctataagt
      541 tatattatca taataccaca aaatcacaaa ttttcattca attatcattt tcaattgcat
      601 tttataaatc attttgtatg taatttggta tgtaagttaa aatgaaaaaa gtagtccatt
      661 ttgaagtaca ttttgtcatt ttgtactcca aaactgaact acttacttgc atatatacgc
      721 ctttatagtg gagtactaca tttacgagat tcggttagag tatcagaaaa agaaaaaata
      781 aaagttacag aaatgtatac ttcaataaat ggtgaattat gtaagattga tagagcttat
      841 tctggagaaa ttgttatttt gcaaaatgag tttttgaagt taaatagtgt tcttggagat
      901 acaaaactat tgccacagag aaaaaagatt gaaaatccgc accctctact acaaacaact
      961 gttgaaccga gtaaacctga acagagagaa atgttgcttg atgccctttt ggaaatctca
     1021 gatagtgatc cgcttctacg atattacgtg gattctacga cacatgaaat tatactttct
     1081 ttcttaggga aagtacaaat ggaagtgatt agtgcactgt tgcaagaaaa gtatcatgtg
     1141 gagatagaac taaaagagcc tacagtcatt tatatggaga gaccgttaaa aaatgcagaa
     1201 tataccattc acatcgaagt gccgccaaat cctttctggg cttccattgg tttatctgta
     1261 tcaccgcttc cgttgggaag tggaatgcag tatgagagct cggtttctct tggatactta
     1321 aatcaatcat ttcaaaatgc agttatggaa gggatacgct atggttgcga acaaggatta
     1381 tatggttgga atgtgacgga ttgtaaaatc tgttttaagt atggcttata ctatagccct
     1441 gttagtaccc cagcagattt tcggatgctt gctcctattg tattggaaca agtcttaaaa
     1501 aaagctggaa cagaattgtt agagccatat cttagtttta aaatttatgc gccacaggaa
     1561 tatctttcac gagcatacaa cgatgctcct aaatattgtg cgaacatcgt agacactcaa
     1621 ttgaaaaata atgaggtcat tcttagtgga gaaatccctg ctcggtgtat tcaagaatat
     1681 cgtagtgatt taactttctt tacaaatgga cgtagtgttt gtttaacaga gttaaaaggg
     1741 taccatgtta ctaccggtga acctgtttgc cagccccgtc gtccaaatag tcggatagat
     1801 aaagtacgat atatgttcaa taaaataact tagtgtattt tatgttgtta tataaatatg
     1861 gtttcttgtt aaataagatg aaatattttt taataaagat ttgaattaaa gtgtaaagga
     1921 ggaattaatt atgaagtgta atgaatgtaa cagggttaaa tacaaccaaa ttttaatttg
     1981 gctttgcgtt ttatcttttt ttagcgtatt aaatgaaatg gttttgaacg tctcattacc
     2041 tgatattgca aatgatttta ataaaccacc tgcgagtaca aactgggtga acacagcctt
     2101 tatgttaacc ttttccattg gaacagctgt atatggaaag ctatctgatc aattaggcat
     2161 caaaaggtta ctcctatttg gaattataat aaattgtttc gggtcggtaa ttgggtttgt
     2221 tggccattct ttcttttcct tacttattat ggctcgtttt attcaagggg ctggtgcagc
     2281 tgcatttcca gcactcgtaa tggttgtagt tgcgcgctat attccaaagg aaaatagggg
     2341 taaagcattt ggtcttattg gatcgatagt agccatggga gaaggagtcg gtccagcgat
     2401 tggtggaatg atagcccatt atattcattg gtcctatctt ctactcattc ctatgataac
     2461 aattatcact gttccgtttc ttatgaaatt attaaagaaa gaagtaagga taaaaggtca
     2521 ttttgatatc aaaggaatta tactaatgtc tgtaggcatt gtatttttta tgttgtttac
     2581 aacatcatat agcatttctt ttcttatcgt tagcgtgctg tcattcctga tatttgtaaa
     2641 acatatcagg aaagtaacag atccttttgt tgatcccgga ttagggaaaa atataccttt
     2701 tatgattgga gttctttgtg ggggaattat atttggaaca gtagcagggt ttgtctctat
     2761 ggttccttat atgatgaaag atgttcacca gctaagtact gccgaaatcg gaagtgtaat
     2821 tattttccct ggaacaatga gtgtcattat tttcggctac attggtggga tacttgttga
     2881 tagaagaggt cctttatacg tgttaaacat cggagttaca tttctttctg ttagcttttt
     2941 aactgcttcc tttcttttag aaacaacatc atggttcatg acaattataa tcgtatttgt
     3001 tttaggtggg ctttcgttca ccaaaacagt tatatcaaca attgtttcaa gtagcttgaa
     3061 acagcaggaa gctggtgctg gaatgagttt gcttaacttt accagctttt tatcagaggg
     3121 aacaggtatt gcaattgtag gtggtttatt atccataccc ttacttgatc aaaggttgtt
     3181 acctatggaa gttgatcagt caacttatct gtatagtaat ttgttattac ttttttcagg
     3241 aatcattgtc attagttggc tggttacctt gaatgtatat aaacattctc aaagggattt
     3301 ctaaatcgtt aagggatcaa ctttgggaga gagttcaaaa ttgatccttt ttttataaca
     3361 ggaattcaaa tctttttgtt ccattaaagg gcgcgattgc tgaataaaag atacgagaga
     3421 cctctcttgt atctttttta ttttgagtgg ttttgtccgt tacactagaa aaccgaaaga
     3481 caataaaaat tttattcttg ctgagtctgg ctttcggtaa gctagacaaa acggacaaaa
     3541 taaaaattgg caagggttta aaggtggaga ttttttgagt gatcttctca aaaaatacta
     3601 cctgtccctt gctgattttt aaacgagcac gagagcaaaa cccccctttg ctgaggtggc
     3661 agagggcagg tttttttgtt tcttttttct cgtaaaaaaa agaaaggtct taaaggtttt
     3721 atggttttgg tcggcactgc cgacagcctc gcagag
//
LOCUS       DAZUMM010000254         3604 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.guided.145, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000254 DAZUMM010000000
VERSION     DAZUMM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.guided.145"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..119)
                     /locus_tag="ACG8E6_002877"
                     /pseudo
     CDS             complement(1..119)
                     /locus_tag="ACG8E6_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
     gene            394..891
                     /gene="dfrG"
                     /locus_tag="ACG8E6_002878"
     CDS             394..891
                     /gene="dfrG"
                     /locus_tag="ACG8E6_002878"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS5063191.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(963..2915)
                     /locus_tag="ACG8E6_002879"
     CDS             complement(963..2915)
                     /locus_tag="ACG8E6_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS5063192.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2922..3158)
                     /locus_tag="ACG8E6_002880"
     CDS             complement(2922..3158)
                     /locus_tag="ACG8E6_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063193.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3396..3604)
                     /locus_tag="ACG8E6_002881"
                     /pseudo
     CDS             complement(3396..3604)
                     /locus_tag="ACG8E6_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
BASE COUNT         1115 a          622 c          583 g         1284 t
ORIGIN      
        1 ttacataatt caccatttat tgaagtatac atttctgtaa cttttatttt ttctttttct
       61 gatactctaa ccgaatctcg taaatgtagt actccactat aaaggcgtat atatgcaagt
      121 aagtagttca gttttggagt acaaaatgac aaaatgtact tcaaaatgga ctactttttt
      181 cattttaact tacataccaa attacataca aaatgattta taaaatgcaa ttgaaaatga
      241 taattgaatg aaaatttgtg attttgtggt attatgataa tataacttat agaaaaaatt
      301 gcttattcaa ctaacgggca ggataatgag aaaaggtttt ttgatagaaa taagaaatta
      361 gatggtaaat tagttaaaaa ggctgtgtta taaatgaaag tttctttgat tgctgcgatg
      421 gataagaata gagtgattgg caaagagaat gacattcctt ggaggattcc caaggactgg
      481 gaatatgtta aaaatactac aaagggacat ccgataatat taggtaggaa gaaccttgaa
      541 tcaatcggaa gagccttacc tgacagaaga aatattattc tgacgagaga taaggggttt
      601 acctttaatg gttgtgaaat tgttcattca atagaagatg tttttgagtt atgtaaaaac
      661 gaagaagaaa tttttatttt cggaggagaa cagatttata atttgttttt cccttatgtt
      721 gagaaaatgt acatcacaaa aatacatcat gaattcgaag gagatacttt ttttccagaa
      781 gtgaattatg aggaatggaa tgaggtattt gcccaaaaag ggataaagaa tgataaaaat
      841 ccgtataact actattttca tgtatatgaa agaaaaaact tattgagtta acgggtgcgt
      901 taattgaaga agaacagtaa agataaagca caagagcttg ggattactcc aagctctttt
      961 tgtcatttga aataaattat atctttccaa tcaaattgtt tatctgtaat ccctaatctt
     1021 tgagcaggtg tcaaaacctt tttatccgct gatttaaaag ggcgacaaaa gttgtagtaa
     1081 gttcttagaa ttgtaatcgc aaattgagca tacttcggat tgaagttagc gtaaatatag
     1141 ctcttcttat caccacgagc tgttgttaat ggtctttcaa gagatgatat ataacgtctt
     1201 atctgttgaa taaagctatt ggtcgaatgg tcattaacat ttaatagcat ctttgcgatt
     1261 tcgtttggtt ccagagcaga taaatccgtt ctacaatcaa ctgaataaaa tcctttatcc
     1321 ttagtggcta atggatgttt gatagggtta tcggcatatt tgcgatatct ttctccatct
     1381 ttactaatag cctcttcgtg gaattgatga cttgttagta attcagttag ataatgagat
     1441 gccaaagtcc gtagaaactt tgttttgaat cctctgatat ctccccaatc gagcagttct
     1501 gcttttgctt gtccgaattc ttttaagcat tgttttctgc ttttcgtctt gttaacttgg
     1561 caaataaaat gatgtgcatc gctcaacctt aattctttcg tgaatactct ataaaaggcg
     1621 tttctaatag agaagtcatc atcactaatc attcgccatt cgtcggcatt aaccatttgt
     1681 ttaattaacc aatagtgagc agttgtagta taaggagcat caacatttaa accatcgaca
     1741 aatctactcc tattagtaat tttccctaat tcgtgaagat aagcattcct attttcgcta
     1801 tcgttagcgg atggttcttg tggataataa gaccaatcta acctatcatt tttacgacta
     1861 aaagtattga gatggtcttc tttgaatttt cttgtatctt catttaattc atccattgaa
     1921 atattccaat cataggcaac atcagaacgg aacacatatc ttgataagac ctcagcagat
     1981 acaactatat acgtttgcat attaaggtct tcaaaccccg tatatttctt tgaaccttgt
     2041 cctttcttac ggacgttatt taggtagtag tgcatcttgt ccgtattaag ccacatctcg
     2101 ttaaatttct ttgtttgaag gggttgtgtc tcatatcgtt ctaaaaactc caaacaacgc
     2161 ctatacagcc actccagctt atgatagtag gttccaactc caattcctaa aatatcacaa
     2221 gtacgactga ctgaaaccct tccaacaacc atcttagcga acattggaag aatggtgttc
     2281 ttttgttggt gataagtagt ggtttcttct ctttttggta gaacattagt gaacttctta
     2341 cacgccttac attgatagcg ttgagatttg cccctacttt tccccctttt gtaaaattgc
     2401 ttcggctcat tgaatggggt tgattcttct tccgagcagc cttctttatg aaattgataa
     2461 tctggctcaa catcttggat gctattgatt tctataaggc gtttaatttc ttcaacaaca
     2521 gaccaattag ataaaggggt tactgtacag ttttgcgata cacctctgcc aataggattg
     2581 tcattgcagt acataccttt atgaccttta tctttggaac taccagtcat tttatatcga
     2641 cttggtttcc cttttacatc tttgaatttg tgctgttctt tcccaaaatt acaacaaaac
     2701 ggattgatgc agtaattata ttgaatggga tattcagaac cgttccattt aaaagacgtt
     2761 ggcaagaaca acattttgtt ataccttgtt gcaaatcgtt ttggagttaa taaatcatat
     2821 tgtttagccc tatcttttaa ttcttcgttt gaaatcggaa ttttaacatc tacaatttca
     2881 tcttccttcg tggctaatct tttaagcttg gtcattgtct atcaacccac tctatctttg
     2941 atacccattt tttcaacaat ccgcttatta cttcgcttgt tggcaatcca ttcaataaac
     3001 ttcttatcgt ctaaaatgtt caataagaat tcatccactg cttcgctttc agtacgctct
     3061 gcatattcag cgtattgctt aactatcacc cttacttgct ctgatatttc ccaatccaca
     3121 gacttagcgt tcttattttt tggctcaaga aattgcatag gaaaatgcac ccctttttgc
     3181 ttagtaaatt gtaaccattt taacacacca atttgcattc ctacaataat tatttcacgt
     3241 tcgcattttt atatacattt taacttacat tttgtggtgt aaattaacaa caaacgttga
     3301 tttaacaata aaaaacgagt atcaaattca gttgcaattt gatactcatt taggtttaca
     3361 ttttcacttt ttgtactcca aaactgaact acttaatatg caagacgttg tctttttttt
     3421 gtatattcaa ttttgaaaac atttccgcaa agttcagacg gacctcgatg tgttgatgaa
     3481 taaaatttat tagtaataac ttctataagg ttatcaatcc ctatattact ttttgcactt
     3541 ccatgataaa gagggaacag agaacaattc tgaaatctta tgctttcctc ttgttcgagt
     3601 tcca
//
LOCUS       DAZUMM010000255         1987 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.guided.172, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000255 DAZUMM010000000
VERSION     DAZUMM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.guided.172"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8E6_002882"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8E6_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFS5063194.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
BASE COUNT          682 a          309 c          410 g          586 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagat
//
LOCUS       DAZUMM010000256         1987 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.guided.173, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000256 DAZUMM010000000
VERSION     DAZUMM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.guided.173"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8E6_002883"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8E6_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFS5063195.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
BASE COUNT          681 a          309 c          411 g          586 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagat
//
LOCUS       DAZUMM010000257         1964 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.guided.174, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000257 DAZUMM010000000
VERSION     DAZUMM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.guided.174"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..378
                     /locus_tag="ACG8E6_002884"
                     /pseudo
     CDS             29..378
                     /locus_tag="ACG8E6_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(650..940)
                     /locus_tag="ACG8E6_002885"
     CDS             complement(650..940)
                     /locus_tag="ACG8E6_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063196.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1148..1840)
                     /locus_tag="ACG8E6_002886"
     CDS             complement(1148..1840)
                     /locus_tag="ACG8E6_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS5063197.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          542 a          374 c          296 g          752 t
ORIGIN      
        1 acttaaaagt tatcttttaa atacttaatt gttaaatacc ttatttgatg caaaagaaat
       61 ctacgatagt caacaaaaaa attttcatta tatccagaaa aatttttctt agttaaggac
      121 ctatggattg cagtaatgca gaattcgtcg aataaactgc caaagacata caatcatatt
      181 gcttttaaaa ttgacgaaca agatatagat tcgtttgtct caaaaatcca aatgttaggt
      241 ttaactgtcg aaccaggacg ttctcgtgtg aaaggcgagg cttcttcaat ttacttttac
      301 gactatgata atcacctctt cgagttacat accggtactc tccaggaacg tttaaaatcc
      361 tacaattcca ctacctgaga ctagtaatga ctagtctctt tttatggcct ctgatccccc
      421 cattgagcga atactaatag ggcttcgaat tgatctgttc aatctttaag caatatccct
      481 caaatgagtt tggaaagttt agtgattcat cacaaatact taaatacatg tagttatata
      541 tagattgagg ttcttgtgag cgtatgctcg tgtgggttca aatcccatcg tctgcatcca
      601 tctttccaaa acaagcggct aggacatttt tgtctcagcc gcttgttttc tagtttattc
      661 tttcatcttg tcggaccttt tttcttgggt atagactttt ctcatattct accacaattt
      721 tgggtttccc taacacatga taggatctca caacaaattg ctttctatta ttataataac
      781 ctgtaacgac taacttcatc ccttcgtcta cttcagctag gaagttcaaa ctatgggaag
      841 caatcaaaca attgacccca tttaaactga aacggatcaa cggtctggca ctaacttgca
      901 aagtacggat cttatctact gttccatatt ttttctccat aaccttcacc tcaataccat
      961 tatacgaaca tacattccct ttgtaaagaa gaaactctgt tttatctttt tgactttttg
     1021 tctattacct ttcttaaaat catattggca cttcctgcga agatgaagga catgtactaa
     1081 aaaaaagagg cagcagtata gattactgtt gcttctttac taatttaggt ttacttgtta
     1141 agaaagatta tttacgcaaa tgttcttcaa caatttgctg agatttatca gctgtatctt
     1201 ccacttcatc ttttgctttt tctaactttt gaatatattg tttcaagctt gagttttcag
     1261 cagttgcttc gtctaatttc ttttgtgtat ctgttaactg agtttctaca tttgtttttt
     1321 cttgtgtcag tgttgcgatt tgttgattca aaccatttac agtattttgt aattctgaaa
     1381 gttttgcttg tgcttcttgt agtcttgctg tccaatctgc attcagatga ttgatttcat
     1441 catttttttg gttgatctca tctagtttga cattgatttc attttgtttg ctttgtagtt
     1501 gtgcatcctt gctgactaat tgactttcta agttttgttt ttctgctgcc gattgatttt
     1561 tttggttttc taattcattt acttttgttt ggtattcttg gacatctgcc tttgaatctt
     1621 gcaatgtctg ttctttttgt gctagtcgag cggtaagatc tgtaatcgct tgctctttat
     1681 ctgtcaatgt atgatcaagc tgtgtcagat tctcattgat tgtatcaata tctgtctctc
     1741 caccccacaa aataatttca tttgcgtacc gcgtgcctgt gtagccaatt agacatagaa
     1801 atgcaacgac aatcccacca gtaataaatt tctttttcat ttgcatagct ccttaatcca
     1861 atacttttac atgctaaaag taagtgatct caaacacaat gtcaagcgtt tttaaattac
     1921 atatgtaatt ttaatataaa tactatcaaa tactcattta atcc
//
LOCUS       DAZUMM010000258         1879 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.guided.177, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000258 DAZUMM010000000
VERSION     DAZUMM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.guided.177"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..431)
                     /gene="dfr"
                     /locus_tag="ACG8E6_002887"
     CDS             complement(<1..431)
                     /gene="dfr"
                     /locus_tag="ACG8E6_002887"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DfrD/DfrG/DfrK family trimethoprim-resistant
                     dihydrofolate reductase"
                     /protein_id="HFS5063198.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKG"
     gene            706..1833
                     /locus_tag="ACG8E6_002888"
                     /pseudo
     CDS             706..1833
                     /locus_tag="ACG8E6_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
BASE COUNT          613 a          322 c          306 g          638 t
ORIGIN      
        1 atcccttttt gggcaaatac ctcattccat tcctcataat tcacttctgg aaaaaaagta
       61 tctccttcga attcatgatg tatttttgtg atgtacattt tctcaacata agggaaaaac
      121 aaattataaa tctgttctcc tccgaaaata aaaatttctt cttcgttttt acataactca
      181 aaaacatctt ctattgaatg aacaatttca caaccattaa aggtaaaccc cttatctctc
      241 gtcagaataa tatttcttct gtcaggtaag gctcttccga ttgattcaag gttcttccta
      301 cctaatatta tcggatgtcc ctttgtagta tttttaacat attcccagtc cttgggaatc
      361 ctccaaggaa tgtcattctc tttgccaatc actctattct tatccatcgc agcaatcaaa
      421 gaaactttca tttataacac agccttttta actaatttac catctaattt cttatttcta
      481 tcaaaaaacc ttttctcatt atcctgcccg ttagttgaat aagcaatttt ttctataagt
      541 tatattatca taataccaca aaatcacaaa ttttcattca attatcattt tcaattgcat
      601 tttataaatc attttgtatg taatttggta tgtaagttaa aatgaaaaaa gtagtccatt
      661 ttgaagtaca ttttgtcatt ttgtactcca aaactgaact acttacttgc atatatacgc
      721 ctttatagtg gagtactaca tttacgagat tcggttagag tatcagaaaa agaaaaaata
      781 aaagttacag aaatgtatac ttcaataaat ggtgaattat gtaagattga tagagcttat
      841 tctggagaaa ttgttatttt gcaaaatgag tttttgaagt taaatagtgt tcttggagat
      901 acaaaactat tgccacagag aaaaaagatt gaaaatccgc accctctact acaaacaact
      961 gttgaaccga gtaaacctga acagagagaa atgttgcttg atgccctttt ggaaatctca
     1021 gatagtgatc cgcttctacg atattacgtg gattctacga cacatgaaat tatactttct
     1081 ttcttaggga aagtacaaat ggaagtgatt agtgcactgt tgcaagaaaa gtatcatgtg
     1141 gagatagaac taaaagagcc tacagtcatt tatatggaga gaccgttaaa aaatgcagaa
     1201 tataccattc acatcgaagt gccgccaaat cctttctggg cttccattgg tttatctgta
     1261 tcaccgcttc cgttgggaag tggaatgcag tatgagagct cggtttctct tggatactta
     1321 aatcaatcat ttcaaaatgc agttatggaa gggatacgct atggttgcga acaaggatta
     1381 tatggttgga atgtgacgga ttgtaaaatc tgttttaagt acggtttata ctatagccct
     1441 gttagtactc cagcagattt tcggatgctt actcctattg tactggagca agcctttaga
     1501 aaagctggaa cagaattgtt agagccatat cttagtttta aagtttatgc accacaggaa
     1561 tatctttcac gggcatataa cgatgctccc aaatattgtg caaatatcgt aaatactcaa
     1621 ctgaaaaata atgaggtcat tattattgga gaaattcctg ctcgatgtat tcaagattat
     1681 cgcaatgatt taactttttt tacaaatggg cttagtgttt gtttagcaga gctaaaagga
     1741 tatcaggtta ccactggcga acctgtttgc cagacccgtc gtctaaatag tcggatagat
     1801 aaagtaagat atatgttcaa taaaataact tagtgcgttt tatgttgtta tataaatatg
     1861 gtttcttatt aaataagat
//
LOCUS       DAZUMM010000259         1086 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.guided.214, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000259 DAZUMM010000000
VERSION     DAZUMM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.guided.214"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..433
                     /locus_tag="ACG8E6_002889"
     CDS             41..433
                     /locus_tag="ACG8E6_002889"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5063199.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            434..>1086
                     /gene="aac(6')-Ie"
                     /locus_tag="ACG8E6_002890"
     CDS             434..>1086
                     /gene="aac(6')-Ie"
                     /locus_tag="ACG8E6_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HFS5063200.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAY"
BASE COUNT          430 a           88 c          198 g          370 t
ORIGIN      
        1 gtatagcatt ttacacagga gtctggactt gactgagttt atggaagaag ttttaattga
       61 tgataatatg gtttttgata ttgataattt aaaaggattt cttaatgata ccagttcatt
      121 tgggtttata gctaaagaaa ataataaaat tataggattt gcatattgct atacactttt
      181 aagacctgat ggaaaaacaa tgttttattt acactcaata ggaatgttac ctaactatca
      241 agacaaaggt tatggttcaa aattattatc ttttattaag gaatattcta aagagattgg
      301 ttgttctgaa atgtttttaa taactgataa aggtaatcct agagcttgcc atgtatatga
      361 aaaattaggt ggtaaaaatg attataaaga tgaaatagta tatgtatatg attatgaaaa
      421 aggtgataaa taaatgaata tagttgaaaa tgaaatatgt ataagaactt taatagatga
      481 tgattttcct ttgatgttaa aatggttaac tgatgaaaga gtattagaat tttatgatgg
      541 tagagataaa aaatatacat tagaatcatt aaaaaaacat tatacagagc cttgggaaga
      601 tgaagttttt agagtaatta ttgaatataa caatgttcct attggatatg gacaaatata
      661 taaaatgtat gatgagttat atactgatta tcattatcca aaaactgatg agatagtcta
      721 tggtatggat caatttatag gagagccaaa ttattggagt aaaggaattg gtacaagata
      781 tattaaattg atttttgaat ttttgaaaaa agaaagaaat gctaatgcag ttattttaga
      841 ccctcataaa aataatccaa gagcaataag ggcataccaa aaatctggtt ttagaattat
      901 tgaagatttg ccagaacatg aattacacga gggcaaaaaa gaagattgtt atttaatgga
      961 atatagatat gatgataatg ccacaaatgt taaggcaatg aaatatttaa ttgagcatta
     1021 ctttgataat ttcaaagtag atagtattga aataatcggt agtggttatg atagtgtggc
     1081 atattt
//
LOCUS       DAZUMM010000260          787 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5063
            SAMEA115707470-rid22576543.guided.231, whole genome shotgun
            sequence.
ACCESSION   DAZUMM010000260 DAZUMM010000000
VERSION     DAZUMM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707470
            Sequence Read Archive: ERR13197199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288033.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288033.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:08:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,891
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,740
            CDSs (with protein)               :: 2,740
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 42 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 28 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707470-rid22576543.guided.231"
                     /isolate="VRE5063"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            174..722
                     /gene="aac(6')"
                     /locus_tag="ACG8E6_002891"
     CDS             174..722
                     /gene="aac(6')"
                     /locus_tag="ACG8E6_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS5063201.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          282 a          116 c          171 g          218 t
ORIGIN      
        1 ttcctgatct ttcctatttt gaataaagtg tttctgtata atttttttat tttataagta
       61 agataaataa tgaaagaata gagatactga ttggtagcta aagatttttg agcgagaaca
      121 tttcgttttt tcaaaaggac tgttagaata agcaagaaca agggggaaga cgtatgataa
      181 tcagtgaatt tgaccgtaat aatccagtat tgaaagatca gctttctgat ttactgagac
      241 tgacttggcc ggaagaatat ggagacagct cggcagaaga agtagaagaa atgatgaatc
      301 cagaacgaat cgcggtagca gcggtagacc aagatgagtt agtaggattt attggtgcaa
      361 tccctcaata cggtatcaca ggttgggaat tgcatccatt agttgtagaa agctcccgac
      421 gaaagaacca aataggtact cgattagtca attacttaga aaaagaagta gcttccagag
      481 gaggaatcac gatttattta ggtacggatg atttagacca tggaacaacg ttaagtcaaa
      541 cggacctgta tgagcataca tttgataaag tggcttccat ccagaacctt cgtgaacatc
      601 cgtatgaatt ctatgaaaaa ttaggttata aaatcgtagg tgtcttacca aatgcaaatg
      661 gctgggacaa accggatatt tggatggcaa aaacgattat tcctcgacca aattctcaat
      721 aataaaaaga ggttgtgaca gaagtaatca gcttcaagaa ataagaagga atttacgaaa
      781 attactt
//
